LOCUS       DAOSGN010000001        14691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_3362_length_14691_cov_3.517764, whole genome shotgun sequence.
ACCESSION   DAOSGN010000001 DAOSGN010000000
VERSION     DAOSGN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 14691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14691
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3362_length_14691_cov_3.517764"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1523..1735
                     /gene="yaaA"
                     /locus_tag="PK087_00005"
     CDS             1523..1735
                     /gene="yaaA"
                     /locus_tag="PK087_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HOH17680.1"
                     /translation="MKIVRIDTEFITLGQFLKFTGLIDSGAMAKVFLQDHFVLINGEK
                     EQRRGKKLYPGTQLSINNEVFRVESQ"
     gene            1732..2823
                     /gene="recF"
                     /locus_tag="PK087_00010"
     CDS             1732..2823
                     /gene="recF"
                     /locus_tag="PK087_00010"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOH17681.1"
                     /translation="MMFHLKRLNIQNFRCYAQFNEQFSPTINILVGNNAVGKTSLVEA
                     VYCAGLGKSYKALNDQELIKNNENYAFVKAEFTEEGKNDEIVFSVVNGKKKIQQNGQI
                     IDRLSDYFGYINVVLFSADDLNLIKGTPAHRREFLDSSISQNNKPYLKALLNYRKYLK
                     QRNEFLKQTDQSTNYDALLLETITEKLIKEAKTIIFERQEFVKALNEKLKTINYEISD
                     EKDCGAIVYVPNRTIDELEKSFKERIQVDLALKTTTIGPHRDDFIPLINQKHAYSFAS
                     SGQQRTLALALKLSLAALCQSQNSRLIIVLDDVFSELDANRQNKIMKYLDKGQQIIIT
                     TTSIEALSPAVIKKSKIMEIIKEERDEQR"
     gene            2810..4726
                     /gene="gyrB"
                     /locus_tag="PK087_00015"
     CDS             2810..4726
                     /gene="gyrB"
                     /locus_tag="PK087_00015"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOH17682.1"
                     /translation="MNNDNKTYGSSNIQILEGLEAVRKRPGMYIGSTGPRGLHHLVWE
                     IVDNSIDEALAGYCTHIEVEIMPNNYLRVTDNGRGIPVGVISKTGESAVEAVFTILHA
                     GGKFDGSSYKVSGGLHGVGASVVNALSENLEVIIKRDGHIFRQEYERGQRKHKIEMIG
                     DSEETGTQVTFKPDIEIFKEGIVFDFETLRFHLQQCAFLNKGLKISLTDSRNPEQPRR
                     VQYCYEGGIKEYVSFLNKSKSPIHSTIIYVFGKDENIEVEIALQYTEDYNSNIYSFIN
                     NIYTPEGGTHEEGFRTSLTRVLNSYGKASNLFRKDESFSGEDVREGLTAIISCRHPNP
                     QFEGQTKTKLGNTEVRRITSQILLDGMDIFLKENPNVAKAIIEKAVVASRARIAAKKA
                     REATRRKSPLETLGFAAKLADCRSKDPALSELYIVEGDSAGGSARQGRNSEFQAILPL
                     RGKVLNVEKARLDKVLGNKEIISMIQALGTGIGDDFSIEQARYHKVIIMTDADVDGAH
                     IRTLLLTFFYRFMKPLVEKGYIYIAQPPLYKITFNKRVEYAYSDGELQKILETFPKSA
                     KPTLQRYKGLGEMNPEQLWETTMDPKARTLLQVNLDDAIEADTVFSMLMGDDVEPRRE
                     FIEENAVYVENLDI"
     gene            4736..7324
                     /gene="gyrA"
                     /locus_tag="PK087_00020"
     CDS             4736..7324
                     /gene="gyrA"
                     /locus_tag="PK087_00020"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOH17683.1"
                     /translation="MENNPKDQNLNHHEEEIDNLDENGHHNGGIKEVNLAREMKTSFL
                     SYAMSVIVSRALPDARDGMKPVHRRILFAMNELGMYADKAYKKSARIVGEVMGKYHPH
                     GDSAIYEAMVRMAQDFSYRYPLVDGHGNFGSVDGDGAAAMRYTEARMSKMAMEVLRDL
                     NKDTVNFIDNYDGSEREPEVLPCRFPNLLVNGAMGIAVGMATNIPPHNLTEVIDGILA
                     LIKNPDISIEGLMTYIKGPDFPTGGCIMGVSQVRKAYMTGNGAITVRAKTETVDLGNG
                     RSAIVVTEIPYQVNKTRLIERIAETAKDKIIEGITGLQDESNREGMRIVIELRRDANP
                     NVILNNLYKHTQLQSTFGINIIALVKGQPKILNLKEVLEVYLNHQIEVLTRRTTFDLE
                     KAEARAHILEGLLIALGNIDLVIQLIKKSKTTEEATNSLMNVFKLTEIQAKAILDMKL
                     QRLTGLEVDKLKTELMETRKFIEECKEILKSHDKKLQIIVNELTEIRDRFGDDRKSEI
                     TLSDDLVIEDEDLIPVEDVIITVTNRGYVKRMTVDTYRAQNRGGKGITGTKMTEEDFV
                     ERLLFTSSHDTLLFFSNFGKVYRLRAFQVPYSSRNAKGLPIVNLLNFEENERLAAVVN
                     INKDNDKEGYLIFATRAGIVKKTEIAAFNNIRTNGIRAILLNEGDELFEVNVTDGEKD
                     IILGASNGKAIRFSESDVRPMGRISAGVRGIKVEKNERVVGMAIVNTDGDEIVIVTEK
                     GYGKRTNVDAFRVQVRGGKGVKALNITEKNGNMVSLSTVRGDEDLMVITDKGMIIRTH
                     LDQILTVGRDTQGVCIIKLNEGHSASTIAIVPRSEEETLVDDEYKEDYELDLDYEFLD
                     EDQDKK"
     misc_binding    7384..7573
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7604..8881
                     /gene="serS"
                     /locus_tag="PK087_00025"
     CDS             7604..8881
                     /gene="serS"
                     /locus_tag="PK087_00025"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOH17684.1"
                     /translation="MIDIKLLRNDLEGTIAKLKQRGADFNYLREVIALDNERRAIIAQ
                     VETLKKERNDLSKQIGLKKRQGEDSTDLLKQVGSIGDKIQADDQKLVQIEKQIEAILL
                     ITPNLLHESVPFGRDEKDNVEIKKYLEPTKFAFTPKAHYELGEDLDILDFGRAAKIAG
                     SRFVVYKGLGARLERALIAFMMDLHSANGYQEIIPPYIANRESMTATGQLPKFEEDAF
                     RLVNGNGDWFLNPTAEVPTINMYRDEVIDIKDLPLNFVSYTMAFRSEAGSAGRDTRGL
                     IRTHQFNKVELIKLTAPEDSYQEHEAMLQESEKVLQTLAIPYRVITLCSGDMGFAMAK
                     TYDIEVWLPSQNTYREIGSISNAEDYQARRGNIRMRRSKDAKLEFVHTLNGSGLAVGR
                     TWVALVENYQQADGSIKIPKALIPYMGCEYIGK"
     gene            8891..10333
                     /locus_tag="PK087_00030"
     CDS             8891..10333
                     /locus_tag="PK087_00030"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOH17685.1"
                     /translation="MLEIINNTKRTTTNKPLVYGAFEKTDTLAIDAKLDDELKELIKK
                     EQISFKLGKINKIFTFGKISHDVLYLIGLGKKEEYSYEQLEESLREVNYKLGDELHFD
                     LASFSLGLNDEEVAKRIVQTIGIYNYHYDECLSEKTTNQLKLKFISEHNLKSALEEAL
                     WLVTAIDNTRDLVNKPYNYLSASDLAEYALHLCASLEDDKVTARIYNKQEIADLGMGA
                     FLGVNKGSTAEPKLIHLVYQNGSQKPIALVGKGLMYDTNGYSLKQSMNNMKDDMAGAA
                     TVLGVFELVVKKQMPINLQLIICATDNRINGEALLPDDVLTAMNKKTIEIVSTDAEGR
                     LTLADAVCFAQKQGCGEVIDIATLTGAVVVALGEYTTGLFGNDQKAIKQMLEAGKKAN
                     EEIWELPINDHIRKQVRSSKVADLTNSTGRTMGASSGAAFIEAFIDKPTKWMHLDIAG
                     TSFHTSPARKEPYGATGVMVKTLYQYLLNQ"
     gene            10427..11821
                     /locus_tag="PK087_00035"
     CDS             10427..11821
                     /locus_tag="PK087_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH17686.1"
                     /translation="MFITIIKWLEISATIVLLLLLFIFVVCLLRAAFIRNKEVQLIQL
                     NNADHNLSKQYLNKLKRLVDIQTSSFDDPEAFHRFREQLKVDYPHIHANFMKEKIDKN
                     AVFTSKVKMEGAPNVLFAAHVDYSSKFQEVKIKNGELYGNGTFDSKSLFFSMFEAVET
                     ILSEQLPLNVNLTIVMTTDDETTKDGINDIVDQFLKKGKFFDLVIEEGSGIVDPSHYG
                     LRSHYALIGVGVTGEAKVKFSTTTANKGERRLNEFLDELPYYRQFKAKIDPKATLPLK
                     KLAKDMPFFERFFFNNLKLFRNYAKRIIDEEYLSLAKLLKTQIIYQDTICTEENCESR
                     IRFEFSTSDTVADVLYGLSKPLRKYGIDYQIQKNIESSRITSTETEGYKQVKQAIKNV
                     FQSLYTAPVILTDISDRRNFDKVSDCVIRFSPLYYPKEALFDGKYGDEHVSGSALYYA
                     VNFFKEILTTYTRR"
     gene            11823..12173
                     /locus_tag="PK087_00040"
     CDS             11823..12173
                     /locus_tag="PK087_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH17687.1"
                     /translation="MYCQHCGNEVKDGDRFCQHCGRPLLGQSQAPEKPKITINKTDKP
                     LVYLTVFALLLTLFPFHNLIVIIGTLVGMGGFLAAALKRNHHNRSLVLALMTLSLTAI
                     IIQLAWFVFINIIL"
     gene            complement(12195..12596)
                     /locus_tag="PK087_00045"
     CDS             complement(12195..12596)
                     /locus_tag="PK087_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOH17688.1"
                     /translation="MCVFCKIVNGEIPAYKVYEDEDFLAFLDIAQASVGHTLVVPKKH
                     FSNFLEADEETISKLFVLAAKLAKQITQNLEVAGFNILNNNGTVAGQSVNHLHVHILP
                     RYEHDSITIKFAQNHLSESEMQALIARISHK"
     gene            12695..13225
                     /locus_tag="PK087_00050"
     CDS             12695..13225
                     /locus_tag="PK087_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOH17689.1"
                     /translation="MGSSRNQQITKRLALLALLMALGVILSLFDKFISSLAFPFLPTA
                     KIGLANIVILLSIYRFSFKESLLLTVMKSALVGLILGSPMSFIISLVASLLSFFGMFA
                     AFKLLSKWASMVSISVIGGFLHIMGQLLTVSLIYRLGNVVFAYGALLVFVSLITSILI
                     GFIALRVIVVFDEKNS"
     gene            13321..14265
                     /locus_tag="PK087_00055"
     CDS             13321..14265
                     /locus_tag="PK087_00055"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOH17690.1"
                     /translation="MYQQIFQKIKEFDTIIIHRHQRPDGDALGSQIGFKEALKTSFPQ
                     KQVFAVGDTNERLAFVGKMDEIADEVYERALVFVLDTAEEFLISDYRYVKGQFLIKMD
                     HHIPRGEFGNLVLVDTNFESCAGLISDFLFQTGMKLNTVAAQALFTGIVTDSGRFRYD
                     SVTAKTLATAAKLLEYDFSLNEVYSNLYIEELKIVRLRAYFTLNFQLTPLGVAYMKNT
                     AADIKNHDVDLFTISRGMVNTMAGIKGIDIWANFTEDESTNTVICELRSNQYNINAVA
                     AKYGGGGHQFASGATLKSFAEADLMLQDLDNLIKENSK"
     gene            14262..>14691
                     /locus_tag="PK087_00060"
     CDS             14262..>14691
                     /locus_tag="PK087_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH17691.1"
                     /translation="MKEKQQIFDLIKQYDDIIIVRHIRPDGDCIGSTIGLRDILRASF
                     PHKQIYSVGNDDSQYLAFLGAEDVVDLTNVIAQALVIVVDTATSDRIDYAFWKEGQYT
                     IKIDHHLRVEDYADLNYVREDLPATAAIITDFWFSFKEELL"
BASE COUNT         4777 a         2663 c         3089 g         4162 t
ORIGIN      
        1 ttctcgtttt ttgaacggca ttgatttaga attcatttga tatcctcctt tttaaaaaaa
       61 tcttctcttt atacatatgt atgtaaaaaa ataaaaccac aaatattatt atacacaaat
      121 aaataaaaat gtaaatacaa aaatacaaac aaacataaaa gaataaataa atatatacat
      181 aaaaccttat taacataaat acaagtttat gtttttatgt atttttgctt gtcttttttt
      241 ataaaactta ttcttggact tgtctttttt ggggatgcca acacaaacaa aacacagatg
      301 gcaattcaaa aaatgcaccg gttttttaaa cgccttttcc accccttaaa aacggcttaa
      361 atttttcgtt ataatcgatt ttgcccacaa actggtaatt ttattagccc ccgggggtaa
      421 aaaacaaacc aagcgaaaaa aattaaggcc caaaaaaggc gttttttggg gctttttttg
      481 gttttttggg agttttaagg caaaaagttg aaaaaaaaag aagatttttg ttttaaaaaa
      541 caacataaat acatataaac ataaaacaat aaatacaact aaacacaaaa acataaaaca
      601 ataaatacaa ctatacataa atacttacaa aaacgtgtct aaaagttttt aaaaattatg
      661 attgtcttga aaatgatcaa aagttgtatt tcaaaatcga gaatattata cattcttaat
      721 cgttctaaat caagccatat gtatgtaaat aagtaagtat ttatttgtgg tttataaaaa
      781 ggcaacggag aaagcaaaaa gatggagtta agaccaatta aatgaataaa attctctaaa
      841 tagctaaata taaccataaa tacataaaaa taaagcaaat taattattaa aaacctaaga
      901 atattatatt taaatgtaag ttaataatat gtaaatacat aaataaataa gcaaatcctc
      961 actaaacgcc accataaagc cataaaacca taaataagca aggaatttaa gaagcaaaaa
     1021 ggcttgatga cttaccacaa acactaaaac acctccataa ataagcaaat cctcactaaa
     1081 cacccccata aaaccataaa accataaata agcaggattt taagaaccaa aaacacctta
     1141 atctatgatt aaaggaacaa aaaagttatt aaacccgcct ataaagccat aaaaccataa
     1201 aaaaagataa gtttacgaaa gaaaatggct gattaaatac tccaaaagac ataaaacaaa
     1261 acctagacta accgtaaata tagcaaacaa acaggaaagc aaaccctaaa aggcccttta
     1321 ttcactcata aaaccatatt tatggcgaaa ataagttatc agtaaccaaa aaaagcccga
     1381 aaaagagtcc atttcttaaa atctccatta aaaaaagaaa gaaggcaaca aaagaaaaat
     1441 aaaccggaaa ataaagcaaa aaagactgga aaaaacaccc catttatgtt ataataagta
     1501 taatttattg aggtaaataa cgatgaaaat agtgagaatt gacactgaat ttattacgct
     1561 cggtcaattt cttaaattta ccggattaat tgattccgga gccatggcaa aagtcttctt
     1621 acaagaccat tttgtcctta taaacggtga aaaagaacaa agacgaggga aaaagcttta
     1681 tccgggaacg caattaagca tcaataatga agtttttaga gtagagagtc aatgatgttt
     1741 catttaaaac gcctaaacat tcaaaatttc cgctgttatg cgcagtttaa cgaacaattc
     1801 tccccaacta tcaatatttt agtagggaat aatgcggttg gcaagaccag tttggttgaa
     1861 gctgtctatt gtgcggggtt aggaaaatcc tataaagccc taaatgatca agaattaatt
     1921 aaaaacaacg aaaactatgc ttttgtgaaa gcggagttta cggaagaggg caaaaacgat
     1981 gaaattgtct tttccgttgt gaacggaaag aagaaaatcc aacaaaacgg gcaaataatc
     2041 gatcgtttaa gtgactattt cggttatatt aatgttgttt tattttcggc cgatgattta
     2101 aacctcatta aagggactcc ggcccaccgc cgtgaatttt tagacagcag tatcagccaa
     2161 aacaacaaac cttatttaaa agcattgtta aattatcgta aatatttaaa acaacgaaat
     2221 gaatttctta aacaaacgga tcaatcgact aattatgatg ctcttttatt agaaacaatt
     2281 acggaaaaac tgattaaaga agcgaaaacg atcatttttg aacggcagga gtttgttaaa
     2341 gcgctaaatg agaagttaaa aaccatcaat tatgaaatca gtgacgaaaa agactgcgga
     2401 gctatcgttt atgttcccaa ccgaacgatt gatgaattag aaaaaagctt taaagaacgc
     2461 attcaagttg atttagcctt aaaaacaacg acgattgggc cgcatcgtga tgattttatt
     2521 cctttgatta atcaaaaaca cgcttattct ttcgcttctt ccgggcaaca acgaaccttg
     2581 gctctagctt taaaattaag tcttgcggcc ctttgccaaa gccaaaattc acgcttaatc
     2641 attgttcttg acgatgtctt tagtgaattg gatgccaacc ggcaaaacaa aatcatgaaa
     2701 tatctcgata aagggcaaca aataatcatt acgacgactt ccattgaggc cttatcgcct
     2761 gcagtaatca aaaaaagtaa aatcatggaa atcatcaagg aggaacgaga tgaacaacga
     2821 taataaaact tacggaagtt ccaatattca aatactggag ggattggaag cggttagaaa
     2881 acggccgggg atgtatatcg gttcgaccgg accgcgcgga ctccaccatt tagtttggga
     2941 aattgttgat aactcgattg atgaagcttt ggctggttat tgtacccata ttgaagttga
     3001 aattatgccg aacaattatc ttcgtgtcac cgataatggg cggggaatcc ctgttggtgt
     3061 catttccaaa accggagaat cagccgttga agctgttttt acaatccttc atgccggagg
     3121 aaagtttgac ggctcatcct ataaagtttc cggtggctta catggtgttg gcgcctcggt
     3181 tgttaatgcc ctaagtgaga atttggaagt aataattaaa cgggacggtc acattttccg
     3241 ccaggaatat gagcgtggac agcgaaaaca taagattgaa atgattggtg actcagaaga
     3301 aacaggaacc caggttactt ttaagccgga cattgaaatt tttaaagaag gcattgtttt
     3361 tgacttcgaa accttacgtt ttcacttaca acaatgtgct tttctaaaca aaggattaaa
     3421 aatttcctta acggattcac gaaatcctga acaaccgcgt cgcgttcaat attgttatga
     3481 gggcggtatt aaagaatatg tcagttttct caacaaatcc aaaagcccga ttcacagtac
     3541 catcatttat gtgttcggga aagatgaaaa cattgaagtg gaaatcgcgc ttcagtatac
     3601 tgaggattat aacagcaata tttattcctt tattaacaat atttataccc ctgaaggggg
     3661 aacacacgaa gaaggtttcc gtaccagttt aacaagagtg ttgaatagtt atggaaaagc
     3721 gagcaattta tttcgaaaag atgaatcttt cagtggtgag gatgtccgcg aaggcttaac
     3781 cgccatcatt agttgtcgac atcccaatcc gcagtttgaa ggacaaacca aaacgaaatt
     3841 aggaaatacg gaagttcgtc ggataacctc gcaaattttg cttgacggaa tggatatctt
     3901 cttaaaagaa aatccgaatg tggcaaaagc gattattgaa aaagcggttg ttgcctcccg
     3961 agcaaggatt gccgccaaga aagcccgtga agcaacacgc cgtaaaagtc cccttgaaac
     4021 attgggtttt gcggccaaat tggctgattg tcgcagcaaa gatcccgctt taagcgagct
     4081 ttacattgtg gaaggtgatt cggccggtgg aagtgctaga caaggacgta acagcgaatt
     4141 tcaggccatt ttgccgcttc gtggcaaggt tttaaatgtg gaaaaagccc gtttggataa
     4201 agtcctcggc aataaagaaa tcatttccat gattcaagcc cttgggaccg gaattggtga
     4261 tgacttcagc atcgaacaag cccgttatca taaagtgatt atcatgaccg atgccgatgt
     4321 tgacggagcc catattcgta ccttgttatt aacctttttc taccggttta tgaagccgct
     4381 tgtggaaaaa ggctatattt atattgccca acccccactt tacaaaatta ccttcaacaa
     4441 gcgggtggaa tatgcctata gtgatggcga attacaaaaa atcttagaaa cttttccgaa
     4501 atcagcgaaa ccaacattac agcgttataa aggtttaggg gaaatgaatc ctgaacaatt
     4561 atgggaaaca acgatggacc ctaaagcccg cactctatta caagtcaatc ttgacgatgc
     4621 gattgaagcg gacaccgttt tttcaatgtt aatgggcgat gatgttgaac cgcggcgtga
     4681 atttatcgaa gaaaatgccg tttatgttga gaatctggac atttaaggag taatcatgga
     4741 aaataatcct aaggatcaaa acctaaatca tcacgaagaa gaaattgaca atttagatga
     4801 aaacggtcac cacaacggtg gaattaaaga agtcaattta gctcgggaaa tgaaaacctc
     4861 gttcttaagt tacgctatga gtgtcattgt ttcccgtgcc cttcctgatg ctagggacgg
     4921 catgaaaccg gttcaccgcc gcattttgtt tgcgatgaat gaattaggta tgtatgccga
     4981 taaagcttat aaaaaatcgg ctcgaattgt cggggaggtt atggggaaat atcaccctca
     5041 tggcgactcg gcgatttatg aagcgatggt gcggatggcc caggatttca gttatcgcta
     5101 tcccttagtg gacggtcacg gtaattttgg ctccgttgat ggtgacggtg ctgcggccat
     5161 gcgttatacc gaagcgcgaa tgagcaaaat ggcgatggag gttttacggg atttaaataa
     5221 agataccgtt aatttcatcg acaattatga cggatcggaa cgcgaacccg aggtattacc
     5281 atgtcgtttc ccgaatttat tggttaatgg ggcgatgggc attgccgtcg gaatggccac
     5341 caacatcccg ccccacaact taacggaagt tattgacggc attctagcct taattaaaaa
     5401 ccccgatatt tccattgaag gactgatgac ttatattaaa ggtccggatt ttccgaccgg
     5461 gggctgtatt atgggcgttt cccaagttcg gaaagcttat atgaccggaa acggcgccat
     5521 taccgtgcgt gctaaaacgg aaaccgtcga tttaggcaat gggcggtctg ccattgttgt
     5581 gacggaaatc ccttaccagg ttaacaaaac gaggttaatt gagcggattg cggaaacagc
     5641 taaagataaa attattgaag gtattacagg cttacaggat gaatccaacc gtgaaggcat
     5701 gcggattgtg attgaattaa gacgcgatgc caacccgaat gtcattttaa ataacttata
     5761 caaacacacg caattacaat ccactttcgg catcaatatt attgctttag ttaagggtca
     5821 gcccaagata ttgaacctaa aagaagtttt ggaggtttat ttaaatcacc aaattgaggt
     5881 attaacacgg cgaacgacct ttgatttaga gaaagcggaa gcgcgagccc atattttaga
     5941 aggtttatta attgcgcttg gtaatattga tctcgttatt cagttaatta aaaagtccaa
     6001 aacgacagaa gaagcaacca acagtttaat gaatgtcttt aaactaacgg aaattcaagc
     6061 gaaagcgatt ttggatatga aattacagcg tttaaccggt ttggaggttg acaaactgaa
     6121 gaccgaatta atggaaacgc gcaaatttat tgaagagtgt aaggaaatct taaagagtca
     6181 cgataagaaa ttacagatta tcgttaatga attaaccgaa atccgcgatc gttttggtga
     6241 tgaccgcaag agcgaaatta ccttaagcga tgatttagtt attgaggatg aggatttaat
     6301 tccggttgaa gatgttatta ttacggtcac caatcgtggt tatgtaaaaa gaatgaccgt
     6361 tgatacttat cgtgctcaaa accggggtgg caaaggcatt accggaacga aaatgacgga
     6421 agaagatttt gtggaaagat tgttgtttac ttccagtcac gataccttgc tgtttttcag
     6481 caacttcggg aaggtttatc gtttgcgggc cttccaagtt ccttattcgt cccgcaatgc
     6541 taaaggactg ccgattgtca atctcttgaa ctttgaagaa aacgaacgct tagcggccgt
     6601 tgtcaacatc aataaagaca atgacaaaga aggttatctc attttcgcaa cccgggccgg
     6661 gattgttaag aaaacggaaa tcgccgcctt caacaacatc agaactaacg gcattcgtgc
     6721 gattttatta aatgaaggcg atgaattgtt tgaagttaat gtgaccgatg gggaaaaaga
     6781 tatcatctta ggtgcttcca acgggaaagc gatccgcttc agtgaaagcg atgttcgtcc
     6841 gatggggcgg atttccgccg gcgttcgggg tattaaagtg gaaaagaatg aacgcgttgt
     6901 cgggatggcg attgtcaaca cggacggtga tgaaattgtc attgtaacgg aaaaaggtta
     6961 tgggaaacgc actaatgttg atgctttccg cgttcaagtg cgtggtggta aaggcgttaa
     7021 agccttaaac attactgaaa agaacgggaa tatggtgtcc ctgtccactg ttcgcggtga
     7081 tgaagattta atggttatta ccgacaaagg gatgattatc cgcacgcatt tggatcaaat
     7141 tttaacagta ggtcgcgata ctcaaggtgt ttgcattatt aaactgaatg aaggccacag
     7201 tgcttccacc attgccatcg ttccaaggag tgaagaagaa acccttgtcg atgatgaata
     7261 caaagaagat tatgaactgg atttggatta tgagtttttg gatgaggatc aggacaaaaa
     7321 ataagcacta tcaacatatt taaattgaca agtttcccca tcccaagtat aatagactta
     7381 atttaatatt cgcagaaaag aaatagtaaa aaatgttcat tgttcagaga gccgctgggt
     7441 ggtgggaagc ggtcaatgat gtttttgaat ccatcttgga gataagcaac agcttcgggt
     7501 agaaccgtta tctttattga gtggagattt ttaatctaac tagggtggca ccgcggatct
     7561 tttcgtccct tattgataag ggactttttt attaggagga attatgattg acattaaact
     7621 attaagaaat gatttggaag ggacgattgc gaaactgaag caaagaggag ccgattttaa
     7681 ttatttgcgg gaagttatcg ctttagacaa tgaaagacga gcgattattg cgcaagtcga
     7741 aactttaaaa aaggagcgga atgatttaag taagcaaatc gggctaaaaa aacgtcaagg
     7801 cgaagacagc acggatttgt taaaacaagt cggaagcatt ggcgacaaaa tccaagccga
     7861 tgaccaaaaa ttagttcaaa tcgaaaaaca aattgaggcg atattattaa ttacgcccaa
     7921 tttattgcat gaaagtgttc cgttcggccg cgatgaaaaa gataatgtag aaatcaagaa
     7981 atatttggag ccgacaaaat ttgcttttac gcctaaagct cattatgaac taggcgaaga
     8041 cttggacatt ttggattttg gtcgggctgc gaaaattgcc ggcagtcgct ttgtggtcta
     8101 taaaggcttg ggtgctcgcc ttgaacgggc cttaatcgcc tttatgatgg atttacatag
     8161 tgctaatggc taccaagaaa tcatcccccc ctatattgct aaccgcgaaa gtatgacggc
     8221 taccgggcaa cttccgaagt ttgaagaaga tgctttccgt ttggttaatg ggaacgggga
     8281 ttggttttta aatccgacgg cggaagttcc caccatcaat atgtatcgcg atgaagtcat
     8341 cgatatcaag gatctaccgc ttaactttgt tagttataca atggcttttc gttcggaagc
     8401 cggcagtgcc ggtcgggaca cgcgcggatt aatccgcacc caccaattta ataaagtgga
     8461 attgattaag ttaacggcac cggaggattc ttatcaagaa catgaagcga tgttgcaaga
     8521 atcggaaaag gttttacaaa ctttagcgat accctaccgg gtcatcactt tatgcagtgg
     8581 cgatatgggt tttgcaatgg ccaaaaccta tgatattgaa gtctggcttc ccagccaaaa
     8641 cacttatcgg gaaatcggca gtatcagtaa tgcggaagac taccaggcac ggcgcgggaa
     8701 tatccgcatg cgccgcagta aagacgctaa attggaattt gtgcacacct tgaacggctc
     8761 cggcttggcc gttggacgaa catgggttgc gctggtggaa aactaccaac aagccgatgg
     8821 cagtattaaa atccctaagg ctctcatccc ctatatgggc tgtgaatata ttggaaaata
     8881 aggaggaata atgttagaaa ttattaacaa cacaaaaaga acgacaacta ataaaccgct
     8941 ggtttatggt gcttttgaaa agaccgatac tttggcgatt gatgcgaagc ttgatgacga
     9001 acttaaggaa ttaattaaaa aagagcaaat cagttttaaa ctgggcaaaa ttaacaaaat
     9061 ttttactttc gggaaaatat ctcatgatgt gctttattta atcggtttag ggaaaaaaga
     9121 agaatacagt tatgaacaac tggaagaaag tttacgggaa gtaaattaca aactcggtga
     9181 tgaactgcac tttgatttag cttccttttc gcttggttta aatgacgaag aagttgcgaa
     9241 acggattgtg caaaccatcg ggatttataa ttatcattat gatgaatgtt tgtcggaaaa
     9301 aacaaccaac caattgaaat tgaagtttat ttcggaacat aacttaaagt cggcgcttga
     9361 agaagccctt tggcttgtta cggctattga taatactcgt gatttagtca ataaaccgta
     9421 caattattta tcagctagcg atttggcgga atatgccctt catctttgtg ctagtttaga
     9481 ggatgacaaa gtaacagcac gcatttataa caaacaagaa attgccgatt taggcatggg
     9541 cgctttttta ggagtcaata aaggctcaac ggcggaaccg aaactcattc atttggttta
     9601 ccaaaacggt agccaaaaac caatcgcttt ggttgggaaa ggcttaatgt atgatacgaa
     9661 cggctacagc cttaaacaat cgatgaacaa tatgaaggac gatatggccg gggcggcgac
     9721 cgttttaggt gtttttgaat tggttgttaa aaagcaaatg ccgattaatt tacagcttat
     9781 tatttgtgcg acggataacc ggattaacgg cgaagcccta ttacctgatg atgtcttaac
     9841 ggcgatgaac aaaaaaacca ttgaaattgt ttccacggac gcggaaggac gcttgacttt
     9901 agccgatgcc gtttgttttg ctcaaaaaca aggttgtggt gaagtgattg atattgcgac
     9961 tttaacagga gcggtcgttg ttgctttagg tgaatacacc accggcttat tcggcaatga
    10021 ccaaaaagcg attaagcaaa tgttggaagc cggaaaaaaa gccaacgaag agatttggga
    10081 actgccgatt aatgatcata ttcgtaaaca ggttcgctcc agtaaagttg ctgatttaac
    10141 caattccacg ggccggacca tgggcgccag ttccggggcg gcatttattg aagcctttat
    10201 tgacaaaccg acgaaatgga tgcatttgga tattgcggga acctctttcc ataccagccc
    10261 ggcgcggaaa gaaccttatg gagcgacggg cgtgatggtt aaaacgcttt atcaatactt
    10321 attaaatcaa taaaaaaagg gccgcgaggc ctttttgttt agtcgttgac atttaagggt
    10381 aatctttgta taattaacat acaaaaatat ggagatgagg attgttatgt tcattacaat
    10441 cataaaatgg ctagaaatca gcgccaccat tgttttgtta ttgttgcttt tcatctttgt
    10501 tgtttgtttg ctgcgggccg cctttattcg caacaaagaa gtccagctta ttcaacttaa
    10561 taatgctgat cataacttaa gcaaacaata cttaaacaaa cttaagcggt tggtggatat
    10621 tcaaacttcg agctttgatg atccggaagc ttttcatcgc tttcgtgaac aattgaaggt
    10681 cgactatccc catatccatg ccaactttat gaaagaaaaa attgataaga atgccgtgtt
    10741 tacttcaaaa gtcaaaatgg aaggagcacc gaatgtttta tttgcggctc atgtcgatta
    10801 tagttcgaag tttcaagaag ttaaaatcaa aaacggcgaa ctttatggca acggcacttt
    10861 tgattccaaa agtttattct tttcgatgtt tgaagcggta gaaaccatct tatccgaaca
    10921 attacctttg aatgtcaatc taacgattgt tatgacgacg gatgatgaaa caacgaaaga
    10981 cggcattaac gatatcgttg atcagttttt aaaaaaaggg aaattttttg atttagttat
    11041 tgaagaaggt tccggaatcg ttgatccctc gcattatggc ttaaggagtc attatgcttt
    11101 aattggggtt ggtgtcaccg gggaagccaa agtcaagttt agcacgacga cggccaacaa
    11161 aggggaaagg cgcctgaatg aatttttaga cgaattacct tattatcggc aattcaaagc
    11221 taaaattgac cccaaagcca ccctcccttt aaaaaagctg gccaaggaca tgcccttttt
    11281 tgaacgcttc ttcttcaata atcttaaact gtttcggaat tatgccaaac gcatcatcga
    11341 tgaagaatat ttatcgcttg caaaactttt gaaaacccaa atcatttatc aagatactat
    11401 ttgtacggaa gaaaactgcg agagccgtat tcgctttgag ttttcaacga gtgatacggt
    11461 ggctgatgtt ttatacggtt tatccaaacc gcttcgtaaa tatggaattg attaccaaat
    11521 ccaaaaaaac attgaatcat cgcggattac cagtacggaa acagaaggtt ataaacaagt
    11581 caaacaagct attaagaatg tcttccaatc cctttatacg gctcccgtta ttttaactga
    11641 tatttccgac cgccgcaatt ttgataaagt cagcgattgc gttattcggt tttcgccgct
    11701 ttattatcct aaggaagctt tatttgacgg aaaatatggg gatgaacatg tttccgggtc
    11761 cgctttatat tatgcagtca acttttttaa agaaatatta acaacttata caaggagata
    11821 gtatgtattg ccaacattgt ggaaacgaag tcaaagacgg tgaccgcttc tgtcaacatt
    11881 gcggtcgacc tttactagga caaagccaag caccagaaaa accgaaaata acgataaata
    11941 agaccgataa accactggtt tatttgacgg tttttgcgct tttgttaacg ctcttcccct
    12001 ttcacaacct cattgtgatt attggaacat tagtcggtat gggaggattt ttagctgcgg
    12061 ctctcaaacg caaccaccac aatcgctcct tggtgttggc gcttatgaca ttgtcattaa
    12121 cggccattat cattcagctt gcctggtttg tgttcatcaa catcatatta taaaaaaagg
    12181 cgcaagggcc ttttttattt atgacttatt cgagcgatga gggcttgcat ttcggattcc
    12241 gacagatgat tttgtgcaaa cttaatggta attgaatcat gttcatatcg tgggagaatg
    12301 tgaacatgga gatggttaac cgattgtccg gcgacggtcc cgttgttgtt gagaatgttg
    12361 aaaccggcaa cttcaagatt ttgggttatt tgtttagcca gcttagccgc taaaacaaac
    12421 aatttgctga tggtttcttc atcggcttcc aaaaaattag aaaaatgttt ttttgggact
    12481 actaaagtat ggccgacgga agcttgagca atatctaaaa aagctaagaa atcctcgtct
    12541 tcatacactt tgtatgcggg aatttccccg ttcacgattt tacaaaacac acacataaaa
    12601 aatgccccct gtgccttatt ataataaata atggttggaa aagcaaccgg aatatgttag
    12661 aatgtaagaa attaacggaa gaaagaaagc gattatgggt agttcacgca accaacaaat
    12721 aacaaaacgc ctggctctat tagctctttt aatggctttg ggagtcattt tgtctttgtt
    12781 tgataaattt atttcctcgc tggctttccc ctttttgccg actgccaaaa taggcttggc
    12841 gaatattgtc attcttttga gcatctatcg cttttccttt aaagagtcct tattgctgac
    12901 ggtgatgaaa agcgccttgg tcggcttgat tttaggaagt cccatgagct ttatcatcag
    12961 tttggtggca tctttactca gttttttcgg catgtttgca gcttttaaac ttttatctaa
    13021 atgggcatcg atggtttcca taagcgtcat cggcggtttt ttgcacatta tggggcaatt
    13081 gctgacggtt tctttaattt accgcttagg gaatgtcgtt ttcgcttatg gcgccctctt
    13141 agtttttgtc tctttaataa cgagcatcct cattggtttc atcgctttac gggtgatagt
    13201 cgtttttgat gaaaaaaact cttaacttgc ttaagaccct tagcttgctt aagggttttt
    13261 attcattttc actgcgctta ccaaaaaata tgttataata tcacgaaacg gaggacggat
    13321 atgtatcaac aaatcttcca aaaaattaaa gaatttgata ccattatcat tcatcgtcat
    13381 caacggcccg acggcgatgc tttaggctcg caaatcggtt ttaaagaagc ccttaaaaca
    13441 agctttccgc agaaacaagt attcgcggtg ggtgatacca atgagcgctt ggcttttgtc
    13501 ggtaagatgg atgagattgc ggatgaagtt tatgagcgtg ccttagtctt tgttttggat
    13561 acggccgaag aatttttaat cagtgactat cgctatgtaa agggccaatt tttgattaaa
    13621 atggaccacc atattcctag aggggaattt ggaaacctcg ttttagttga taccaatttt
    13681 gaaagttgtg ccggactcat cagtgatttt ttgtttcaaa caggaatgaa gcttaatacg
    13741 gtggcggcgc aagccctctt tacgggaatt gtgaccgaca gcggtcgctt tcgttatgat
    13801 tccgttacgg ccaaaacctt ggcgacggcg gctaaacttt tggaatatga tttttcctta
    13861 aatgaagttt acagcaattt atacattgaa gaattaaaga ttgtccggtt gcgggcttat
    13921 ttcaccctaa actttcaatt aacgccgctg ggggttgctt atatgaaaaa cacggccgcc
    13981 gacattaaaa atcacgatgt cgatttgttt accatttccc gcgggatggt caataccatg
    14041 gccggcatta aagggattga tatctgggct aatttcaccg aggatgagag cactaacaca
    14101 gttatttgtg aactgcgttc caaccaatac aacattaatg ccgttgctgc caaatatggt
    14161 gggggtggcc atcaatttgc cagcggggcc acgttaaaat cgtttgcgga ggcggattta
    14221 atgttacaag atcttgataa cttaataaag gaaaacagca aatgaaggaa aaacaacaaa
    14281 ttttcgattt aatcaaacaa tatgatgata ttattattgt tcgtcacatt cgtcccgatg
    14341 gcgactgtat aggaagcaca atcggactgc gggatatttt aagagcgtct tttccccata
    14401 aacaaattta tagtgttggc aacgacgaca gccaatactt ggcttttctt ggtgctgaag
    14461 atgttgtcga tttaacgaat gtgattgcgc aggctttagt gattgttgtt gataccgcca
    14521 ccagtgaccg cattgattat gcgttttgga aagaaggtca atataccatt aaaattgatc
    14581 atcatcttcg ggtcgaagac tacgccgatt tgaattatgt gcgcgaggat ttacctgcca
    14641 cggcggcgat tattaccgat ttttggttta gttttaaaga ggagctcctt a
//
LOCUS       DAOSGN010000002        11809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_4580_length_11809_cov_3.867875, whole genome shotgun sequence.
ACCESSION   DAOSGN010000002 DAOSGN010000000
VERSION     DAOSGN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4580_length_11809_cov_3.867875"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PK087_00065"
     CDS             <1..794
                     /locus_tag="PK087_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH17692.1"
                     /translation="RAGIKDQNRPIGSFLFLGPTGVGKTEVARSLADALFDDEHHIVR
                     IDMSEYMEKFAVSRLVGAPPGYVGYEEGGQLTEKVRRNPYSIVLFDEIEKAHADVFNI
                     LLQILDEGRLTDSQGRTVDFKNTIIIMTSNLGSGLLLNNENMEEIQKLLRITFRPEFL
                     NRIDEVVIFKPLSQATQMKIVEKMLNELANRLKQDRLNIVFTDRAKQFVIDSSFSHEY
                     GARPIKRFIQKEIETMLAKEIIKGALVPERPYIVDVVNEQFVIKS"
     gene            889..1734
                     /gene="folD"
                     /locus_tag="PK087_00070"
     CDS             889..1734
                     /gene="folD"
                     /locus_tag="PK087_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOH17693.1"
                     /translation="MILDGKKLAETITKNIADEVSQIKEKITLALILVGKNPASQVYV
                     HNKQKACAEVGINFEAYFLDENIPQKEVADIIEKCNANPDVHGILVQMPLPSHIDADA
                     IINLIDPIKDVDGLTTINQGKLFTNQNPIVPATPKGVMTLLQRYFIDIAGKNAVVVGR
                     SKLVGKPLAMLLLDKNATVTMAHSKTSNLKEVTKRADLLFVAVGSPKFITADMVKKDA
                     VVVDVGINKVEGKLIGDVDFEGVKDFVSYITPVPKGVGPMTIASLLENIVDCYHRQKA
                     IKDAN"
     gene            1788..2345
                     /gene="pth"
                     /locus_tag="PK087_00075"
     CDS             1788..2345
                     /gene="pth"
                     /locus_tag="PK087_00075"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOH17694.1"
                     /translation="MKLIVGLGNPGLRYKKTRHNVGFMFVDSFLKTVKQKTTKNKNLK
                     SEIALFNYQNDKLIIIKPQTFMNLSGEAVFLTMKYYQLRLDDVLVIYDDMDLPEGKLR
                     IRPSGSAGGHKGMANIMEFLKTDQIKRLRLGIGKDENIEAVDYVLGVFSAQGRSLIKA
                     VTDRSADIITDYLNLPFVDFMSRYN"
     gene            2354..5836
                     /gene="mfd"
                     /locus_tag="PK087_00080"
     CDS             2354..5836
                     /gene="mfd"
                     /locus_tag="PK087_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOH17695.1"
                     /translation="MNNVITLFSRNQRVKAVGEQLIHPHYRYLVNELSPHHGYLLTYF
                     AFLQSSQFVIYVASNLFQANRAYEKFCTLAGMENVNLYLVDEIVSAELVAVSGDFKFE
                     RLSTIKSALDKQPKIIVTHTQAALRPLLEKSRLEKSVKTFRCGQEINLEKLAKELISL
                     GYQKTPTTTAVGEFSVRGEVVDIFPPFYDSPVRIGLFDVEIEQIKTFDVESQRSIKTI
                     DEVVIFPMQELIYDEKQKAAFFHHLHKDLSQISSWPRFLENDVMDLENYHNIDRLNKY
                     IRYFTNSPLTILDYFDEKIVFYEEYSRLQDSYQQIVLDLETYLNHLEKPEPLDLFFFF
                     DFYNLFHRAPRQIFLSEFKKSLNDVAIDCVFPLKGYSVIDYQNDLRNLVADVQANKSK
                     TFVFACSQAERIGLLKEIFKEAEIKFTEPTSFLEIKPEQVHIMLIENALSFGFFEDHL
                     EVLTEAEIFKTLKKSKARFRSMMQNTQVISSRDDLQSGDYVVHYDYGIGKYLGIKTIE
                     LKEIRNDYIALAFANMEIYIPVEKINLLEKYQGSEGTIPKLTNIGKGEWEKKKRKIQE
                     KLETMAGDLISLQAEREQNPGFKYPPDSDLQSLFEEDFVYEETPDQLKAIAQVKADME
                     KGLVIDRLICGDVGYGKTEIAMRIAFKTVFSHKQVACLAPTTILTRQHFHSFKERFDK
                     YGIRVELLNRLVSIDKQKQILADMKKGLVDIVIGTHRLLSEDIVFNDLGLLIIDEEQR
                     FGVIHKEKIKQMKVNVNVLTLTATPIPRTLQMSIMGLRQLSLIETPPKDRYPVQTYVV
                     EENEMIVKEAIYRELAREGQVFYLHNRVVDLDRLYRKLRRLVPEARIGVVHGQMSKMA
                     MENAIQAFIDKEFNVLLCTTIIETGIDIPNTNTLIISRADYLGLAQMYQIRGRVGRTD
                     RVAYAYLMYDEDKVLTADSTKRLSAIKEFTNLGSGYKIAVRDLAIRGAGDILGKEQSG
                     FIDAVGFDMYMKMLNDAMNKVSGHEAPKEETPIPIAVSKHVDPAYVSDDDIKIYIHKE
                     IAQVKTKTDKEQLISVLTDRFGRLNEEILTYIEERYLQTVLKALAINGVLETENTVVV
                     AIPRRMSALVNAEVLFMRASALSRDIHFEYKNQQIIVKMNKKPQTKQWIFWLTALLEG
                     EIWKKKAVEELSDS"
     gene            5873..6733
                     /locus_tag="PK087_00085"
     CDS             5873..6733
                     /locus_tag="PK087_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH17696.1"
                     /translation="MQKIAVISDVNAGLDYLGYDPDIPVLRSIINFGEEHFIDGIDIK
                     ADAFYKKLQTSPIIPSTSAPTVGEAMTLLDKLISEGYTDVIMISISYQLSSIGQMVET
                     LIDEYANKIKIHVIDSKSATYMQGYIAVEAKKMAVAGKPLEEILDYCHYLVENKHAYF
                     VVDDLSYLVKNGRLSGAAGFIGSLLKVKPVLELTKEGKIVSKEKIRTHSKAVERALEL
                     FKNEIKDAKKVKIFVFHTVREQDAKILVNRLQKECKNAEEIELHMVTPAVGAHIGCGI
                     IGMGYFILEK"
     gene            6869..7411
                     /locus_tag="PK087_00090"
     CDS             6869..7411
                     /locus_tag="PK087_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17697.1"
                     /translation="MKKIQCLNCLKVSVFNDVCPQCESNNIIEIHKKGIVYQKRFFTH
                     KEWLARLSTASQWAIMPINRSYLKQIIQTNLRRSRRERFLFPFVVLFGSGIIGLIASL
                     MVKNNQTLIDGNAFNVMVAGLAFSSLLLVFSGVFLYWLITNQALLMLWKKHKIRFVRF
                     TKKEMKTLLKEINDEKTLSQ"
     gene            7389..7940
                     /locus_tag="PK087_00095"
     CDS             7389..7940
                     /locus_tag="PK087_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH17698.1"
                     /translation="MKKRSVNNLKITNNHLLIRPFQRRDHDDLWQLFSNERVVRFLGV
                     KQFHDKIAVERYLNECEQEYERQEIYRLGIELLAEQKIIGFIGVSRYDLTKTSAQVVY
                     GLAEAYWHRGLMVEALQLFVDYLTKELHKEIIIGTHIDENQASGKVLLKAGFKRDVDY
                     DQMMLIKGVNQHLTGYSLKTKGE"
     gene            7942..8460
                     /locus_tag="PK087_00100"
     CDS             7942..8460
                     /locus_tag="PK087_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17699.1"
                     /translation="MKKIILILIMMMLGLLIACGKQPGELRNVSNIATSEIFNQKETK
                     YYVYFHRLDCPDCDESAPYVINYSQIIKEYEGCRNKRKIYAVLLFTASEKPGRSTYIY
                     REYTGEGGQGTNGKYFVDGVRAWEDLYIATTSSLISISENTDGVKVAKYVAQGAEAVT
                     RALNEQLGTCYQ"
     gene            8460..9206
                     /locus_tag="PK087_00105"
     CDS             8460..9206
                     /locus_tag="PK087_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17700.1"
                     /translation="MKDTYITKSERTFQNLNYSLYSDGTSHPLLFFFHGFTSNRHLGI
                     MGRGEVLARLGFTVVAFDAPYHGDRDNGTFSQLSVSARQKELMEIVIQNAHEAKWLYE
                     KHLQAEQLISKAPVFAFGVSMGAQVCFYLATIMPKLQTIVTLVGSPSFCDFYRWKQQQ
                     YQWPDDLEFKIKLEKYQSLDPLIHSERLQNTNIFMGVGTQDDVVPKHYAKQLAEKLKT
                     SVYQEYETAHFSTPKMLDDAYCFLKKTLTF"
     gene            complement(9218..9871)
                     /locus_tag="PK087_00110"
     CDS             complement(9218..9871)
                     /locus_tag="PK087_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17701.1"
                     /translation="MKKILLLLILTILAGCQDDPLYQYDKQNPHQLNIALCFTPEEVV
                     LLDENDVYTLDWTYEDAQLIIRSETLNCFIAGEYRINITDSKNTLSVKLEISGFNQNI
                     PSLLKEELFEKPTSFLVFFSKANCYGCELIMADIYRYHDAQTARNHEPLLPIWQIKYE
                     DNHNASLFGPVESVLGVSKLSDLQIPSVPTLLLIKNHIVVAYYHGASEVLNYLNTLK"
     gene            9955..10404
                     /locus_tag="PK087_00115"
     CDS             9955..10404
                     /locus_tag="PK087_00115"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOH17702.1"
                     /translation="MKKHLWTILLIIWISFIFLNSFQTGAQSSQASGIFTNFFTNLFL
                     KIGWSVNQETLSMLIRKGAHMFEFFVLALLFCLQYFRKHPLQKTLEKALISSIVVAVV
                     DELIQLSVPGRVGTIIDVAIDNVGIVFALVLFWLIVRHKQKKSANSS"
     gene            10465..11343
                     /locus_tag="PK087_00120"
     CDS             10465..11343
                     /locus_tag="PK087_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH17703.1"
                     /translation="MKKLNIFLITILGFLLVGCKATDIDVFRFVNHEITIQVGDDAVL
                     NLIYGEYDADDEVTYTLSKEGIIVLFRNTATGIAPGEVTVTATIDGIKKTNIIVTVIN
                     EPINSMSISSAAFVTVGTPIQLAVSVLPSHLSNEVTWSIEDSVQSDVEAATVSPSGLL
                     SGVIGATTKAELTAGGKQIVVVATSKVDPLMSVKKTILVKYQPTTKVTLSTAGGKTTM
                     GVTDTLQLVATILPAHACPVLTYTSSNTSVVTVSNTGLVSVPENSTNYKTTTITATSL
                     DNVVGTIDITVLDPNA"
     gene            11358..11633
                     /locus_tag="PK087_00125"
     CDS             11358..11633
                     /locus_tag="PK087_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOH17704.1"
                     /translation="MKYCANCGNQLEDEAAFCPQCGTRQDGKFAAKTANNDAPSVGYA
                     ILSFLIPLVGLILFLNWKNNFPRRAKSCGIAALIGFIINFVVFFYLP"
BASE COUNT         3680 a         1997 c         2493 g         3639 t
ORIGIN      
        1 aacgcgccgg cattaaagat caaaatcgac cgattggctc cttcctcttt ttgggaccta
       61 ccggtgttgg taaaaccgaa gtggctcgca gtttagcgga tgctttattt gatgatgaac
      121 accatattgt gcggattgac atgagtgaat atatggagaa gtttgcggtc agccgtttgg
      181 ttggagcgcc accgggttat gtcggttatg aagaaggggg ccaattaacg gagaaagtca
      241 gaagaaatcc ttattcgatt gttctgtttg atgaaattga aaaagcgcat gccgatgtct
      301 ttaatattct actgcagatt cttgatgaag gccgtttaac cgattcccaa ggtcgaacag
      361 tggattttaa aaatactatc atcatcatga cttccaattt aggaagtggg cttttgctta
      421 acaatgaaaa tatggaggaa attcaaaagt tgttgcgaat aacctttcgg ccggaatttt
      481 tgaatcggat tgatgaagtt gttatcttta aacctttgtc tcaggcaaca caaatgaaaa
      541 ttgtggaaaa gatgcttaat gaattggcga accgcttaaa gcaggaccgt ctgaatattg
      601 tttttacgga ccgtgctaaa caatttgtca ttgattcgag cttttcccat gaatatggtg
      661 ctcggccgat taaacgattt atccaaaaag aaattgaaac catgttagct aaagaaatta
      721 ttaaaggtgc tttggttccg gaacgcccat atatcgttga tgttgttaat gaacagtttg
      781 tgattaagtc ttaagttttt tcttaagact tttttaaaac catagaaata ttattttgat
      841 tggtgtataa tgataattga ctgaaaaaaa aagaaatgga gcgtatttat gattttagat
      901 ggtaaaaaac ttgcggaaac aatcaccaaa aacattgctg atgaagtctc acaaattaaa
      961 gaaaaaatta ctctcgcctt aattcttgtt ggtaaaaatc cggccagcca agtttatgtt
     1021 cataacaaac aaaaggcctg tgcggaagtt ggtattaatt ttgaagctta ttttttggat
     1081 gaaaacattc cccaaaaaga agttgccgac attattgaaa aatgcaatgc caaccctgat
     1141 gttcatggca ttttagtgca aatgccctta ccgtcccata ttgatgccga tgcaattatc
     1201 aatctcattg atcccattaa agatgttgac ggcttaacca cgattaatca aggaaaactc
     1261 tttaccaatc aaaatccgat tgttccggcg accccgaaag gtgttatgac cttactacag
     1321 cgttatttca ttgatattgc cggaaaaaat gcggttgtgg ttgggcgcag taaattagtc
     1381 ggtaaacccc ttgccatgct cctcctcgat aaaaacgcca cggtgacaat ggcacactcc
     1441 aaaacaagta atttaaaaga agtgacgaag cgtgccgatt tattatttgt ggcggtgggc
     1501 agccctaaat ttatcacggc ggatatggtt aaaaaagatg ccgttgttgt cgatgtaggc
     1561 ataaacaaag tggaaggaaa actcattggc gatgttgatt ttgaaggtgt taaagacttt
     1621 gtttcctata tcacccctgt tccgaaaggc gtgggtccga tgacgattgc ttcccttttg
     1681 gaaaacattg tcgattgtta ccatcgccaa aaagctatta aagacgctaa ttaaaacatt
     1741 aaagtccata actgcttatg gacttatctt ttcataagga gaaaaagatg aaattaattg
     1801 ttggtttggg gaatcccggc ttgcgttaca aaaaaacacg ccataacgtt ggatttatgt
     1861 ttgtggacag ttttttaaaa accgttaaac aaaaaacaac gaaaaacaaa aacttaaaat
     1921 ccgagatagc tctttttaat tatcagaatg acaaattaat tattattaaa ccccaaacct
     1981 ttatgaattt gtccggggaa gccgtctttc tgaccatgaa atattatcaa ctgaggctgg
     2041 atgatgtgtt ggttatttat gatgatatgg atttgcccga aggaaaacta agaattcgtc
     2101 ctagcggctc agcaggtggt cacaaaggga tggcgaacat tatggagttt ttgaaaaccg
     2161 accaaattaa acgtttgcgt ttaggcatcg gtaaagatga aaacatcgaa gcggtcgatt
     2221 atgttttggg tgtttttagt gctcaaggaa gaagccttat taaagccgtt actgaccgca
     2281 gtgccgatat tattaccgac tacttaaact tgccgtttgt tgattttatg agccgctaca
     2341 attagaggtc aagatgaata atgttattac attatttagt cgtaaccaac gcgttaaagc
     2401 cgttggcgaa caactaatcc atcctcatta tcgttatctg gttaacgagt taagccccca
     2461 ccatggttac ttgttaacct attttgcgtt tcttcagtcg tcacaattcg tgatttatgt
     2521 tgccagtaat ttatttcaag cgaaccgcgc ttatgaaaag ttttgcacat tggcgggcat
     2581 ggaaaatgtc aatttatatt tagtggatga aattgtgtcg gcggaactgg ttgccgtcag
     2641 cggtgacttt aaatttgagc gcctcagcac gattaaaagt gctctggata agcagcctaa
     2701 aattattgtt acccatacgc aagcagcctt aagaccgctt ttggaaaaaa gcaggttgga
     2761 aaaaagtgta aaaacttttc gctgtggtca agaaataaac ttagagaagt tggcgaaaga
     2821 attgataagt ttaggttatc aaaaaacacc gacaacgacg gctgttggtg agttcagcgt
     2881 tcggggggaa gtggtggata tctttccgcc cttctatgat tctcctgttc gcatcggtct
     2941 ttttgatgtc gagattgaac aaataaaaac ttttgatgtg gaaagtcaaa gaagtattaa
     3001 gaccatcgat gaagtcgtca tttttccgat gcaggaatta atctatgacg aaaaacaaaa
     3061 agcagccttt tttcatcacc ttcataaaga cctaagtcaa atttcttcct ggcctcgttt
     3121 tttggaaaac gatgtaatgg atttagaaaa ctatcacaac attgaccgtc ttaataaata
     3181 tattcgttac ttcaccaact ctccgcttac aatccttgat tacttcgacg aaaaaatcgt
     3241 cttttatgaa gaatattccc gtcttcaaga cagctatcaa cagattgttt tggatttgga
     3301 gacttatttg aatcatttgg aaaaaccgga acccctggat ttatttttct tttttgattt
     3361 ttataatttg tttcatcggg cgccgcgcca aattttttta agtgaattta aaaagagttt
     3421 aaacgatgtt gccatcgatt gtgtttttcc tttaaaagga tatagcgtta ttgattatca
     3481 aaatgatctt cgtaatttgg tggccgatgt tcaagctaac aagagcaaaa ccttcgtttt
     3541 tgcttgttcc caagcggaac gaatagggct tttaaaggag atttttaaag aagcggaaat
     3601 taaatttacc gaaccaacta gttttttaga aataaaaccg gaacaagttc atatcatgtt
     3661 aattgagaac gcccttagtt ttggtttttt tgaagatcat ttggaagttt taacagaagc
     3721 agagattttt aaaacactga aaaaaagcaa agcgcgtttt cggagcatga tgcaaaacac
     3781 ccaagttatt tcttcacggg atgatttaca aagcggcgat tatgttgtcc attatgatta
     3841 tggaatcggg aagtatttag gaattaaaac cattgaacta aaagagattc gtaatgatta
     3901 tattgccttg gcttttgcta atatggaaat ctatattccg gtggaaaaaa tcaatttact
     3961 ggaaaaatac caagggagcg aaggaacgat tccgaaatta acgaatatcg ggaaaggtga
     4021 atgggaaaag aagaaacgca agattcagga aaaactggaa accatggccg gcgatttaat
     4081 ttcattacaa gccgagcggg aacaaaatcc cggcttcaaa tatccgccgg atagtgattt
     4141 gcaaagcttg tttgaagagg attttgtgta tgaagaaacg ccagatcaac tgaaagccat
     4201 cgcccaagtt aaagccgata tggaaaaagg tctggtgatt gatcgcctta tttgtggtga
     4261 tgtcggttat gggaaaacgg aaatcgccat gcggattgct tttaaaacgg ttttttccca
     4321 taaacaagtc gcttgtttgg cgccgacaac gattttaacg cggcaacatt ttcatagttt
     4381 taaagagcga tttgataagt acggtattcg cgtggagctt ttaaatcgct tagtcagtat
     4441 tgataaacaa aaacaaattc ttgcggatat gaaaaagggc ttggttgata ttgtcatcgg
     4501 cacccatcgt ttattaagcg aagatatcgt ttttaatgat ttaggcttgt taattattga
     4561 tgaagaacaa cgttttggtg ttatccataa agaaaagatc aaacaaatga aagtgaatgt
     4621 taatgtgtta actttaacgg ctacaccgat tccccgcacc ttgcaaatgt cgattatggg
     4681 gcttaggcag ttaagtttaa ttgaaactcc gcccaaggat cgctatcccg ttcagactta
     4741 tgttgtggaa gaaaatgaga tgattgtgaa agaagcgatt taccgtgaac tggcccggga
     4801 gggtcaagtg ttttatctac acaaccgggt tgttgattta gaccgtttat accggaaatt
     4861 aagaagactg gttccggaag ctagaattgg tgttgtccat ggacaaatga gtaaaatggc
     4921 gatggaaaat gccatccaag ctttcattga taaagaattc aatgttttat tgtgtacaac
     4981 gattattgaa acgggcattg atattcccaa cacgaacacc ctaatcatca gtcgagctga
     5041 ttatttgggc ttagctcaaa tgtatcaaat cagagggcgc gttggtcgga ccgaccgcgt
     5101 tgcctatgcc tatttgatgt atgatgagga taaagtgtta acggccgact cgacaaaacg
     5161 cttatcggcc attaaggaat ttaccaattt aggaagtggc tataaaattg cggttcggga
     5221 cttagccatc cgcggggccg gtgatatttt aggcaaggaa caatcgggtt tcattgatgc
     5281 agtcggattt gatatgtata tgaagatgtt gaacgatgcg atgaataaag tatccgggca
     5341 tgaagcaccg aaagaagaaa cgccgattcc gattgccgtt tccaaacatg ttgatccggc
     5401 ttatgtcagt gatgatgaca ttaaaatcta tattcataaa gagattgccc aagtgaaaac
     5461 gaagactgac aaagaacaac tcattagtgt tttaactgac cgttttggaa gactaaacga
     5521 agaaatctta acttatattg aggaacgcta tttacagact gtcttaaaag cattggcgat
     5581 taatggggtg ttggaaaccg aaaacacggt cgttgtggcc attcccagac gaatgtcggc
     5641 tttagttaat gcggaggttt tgttcatgcg ggcgagtgct ttaagtcgag acattcattt
     5701 tgaatataaa aaccagcaaa taattgtgaa aatgaacaaa aaaccccaaa caaagcagtg
     5761 gattttttgg ttaacagcct tacttgaagg agaaatttgg aaaaagaaag cagttgaaga
     5821 attaagcgat tcatgatata atcacaactg gaataatgaa aaggagataa atatgcaaaa
     5881 aatagctgtc atttccgatg ttaatgccgg tttagattat ttaggatatg atcccgacat
     5941 tccagtcttg cgttcgatta ttaactttgg tgaagaacac tttattgatg gtattgatat
     6001 taaagcggat gctttttata aaaaacttca aactagtcca attattccgt caacatccgc
     6061 gccaacggtt ggtgaggcta tgactttatt agacaaacta atcagtgaag gctataccga
     6121 tgtcatcatg atttccattt cttatcagtt aagctcaatt ggccaaatgg tggaaacgct
     6181 tattgatgag tatgcgaata aaattaaaat tcatgtgatt gattcgaaaa gtgcaactta
     6241 tatgcaaggc tatatcgctg ttgaagcaaa gaaaatggcg gtcgccggca aaccgcttga
     6301 ggaaatttta gattattgcc attacttagt ggaaaacaaa catgcttatt tcgtagttga
     6361 tgatttatcc tatttggtta aaaacggccg tttaagcggt gccgccggtt ttatcggcag
     6421 tttgttgaaa gttaaaccgg ttttggaact cactaaagag ggaaaaattg ttagcaagga
     6481 aaaaatccgc actcattcca aagcggttga acgcgcttta gaattattta agaatgagat
     6541 taaggatgcg aaaaaagtaa agatttttgt ttttcatacc gttcgtgaac aagatgcgaa
     6601 aatcttggtt aaccgtttgc agaaagaatg taagaacgcc gaggaaatcg aattacatat
     6661 ggtgacaccg gcggttggag ctcatatcgg ttgtggaatt attggaatgg gatattttat
     6721 tctcgaaaaa taaagacaac ttttaccatt cgcaatatta tttaggcctt aattgtaaaa
     6781 caccggtttt taaaactggt gttttttaaa atcgtaaaca agatttttta ttgaattttt
     6841 tctttgttta gcgtataatt tatcaagtat gaagaaaatc caatgcttaa attgtttaaa
     6901 ggttagtgtt tttaatgatg tttgtccaca atgcgaaagc aataacatca tcgaaattca
     6961 taaaaaaggc atagtttacc aaaagcggtt ttttactcac aaagaatggt tagcacgctt
     7021 aagcacggct agccaatggg ctataatgcc tattaatcgc tcctatctta aacaaattat
     7081 ccaaactaac ttaaggcgca gtcgtcggga aagattcttg tttccttttg ttgtgctttt
     7141 tggaagtgga atcatcggtt taattgcttc cttgatggtt aaaaacaatc aaacgctgat
     7201 tgacggtaat gcttttaatg ttatggtggc ggggctagct tttagtagtt tgttgctggt
     7261 tttttccggt gtttttcttt attggttaat caccaaccaa gcacttttaa tgctttggaa
     7321 aaaacacaaa atccgctttg ttcgcttcac aaaaaaagaa atgaaaacat tattgaagga
     7381 aataaatgat gaaaaaacgc tcagtcaata acttgaagat aacgaacaat catttattga
     7441 ttcgcccttt tcaaaggcgg gatcatgatg acttatggca actgtttagt aatgagcgtg
     7501 ttgttcgctt tttaggtgtt aagcaatttc atgataaaat tgctgtagaa cgctatttaa
     7561 atgaatgtga acaggaatac gaacgccaag aaatttatcg cttaggaatc gagttgttgg
     7621 ccgaacaaaa aatcatcggt tttattgggg tctcgcgtta tgatttaacg aaaacaagcg
     7681 cccaagtcgt ctatggttta gcggaagcct attggcatcg gggcttaatg gttgaagctc
     7741 tccaattgtt tgtggactat ttaactaaag aacttcataa agaaattatc atcggcactc
     7801 atattgatga aaatcaggct tcaggaaagg tactcctaaa agccggtttc aaacgagatg
     7861 tcgattatga tcaaatgatg cttatcaaag gcgttaatca acatttaacc ggatattctt
     7921 tgaaaactaa aggagaatga tatgaaaaaa ataatcttaa tattaattat gatgatgctt
     7981 ggattattga ttgcttgtgg caaacaaccc ggtgaactaa ggaatgttag taatattgcg
     8041 acttcggaaa tctttaacca aaaagaaaca aaatattatg tttattttca tcgtcttgat
     8101 tgtccggatt gtgatgaatc cgccccttat gtcattaatt attcacaaat tattaaagaa
     8161 tatgaaggct gtcgcaacaa acgcaaaatt tatgcggttt tattgtttac agcttccgaa
     8221 aaaccagggc gttcaaccta tatttatcgc gaatataccg gtgaaggcgg tcagggaacg
     8281 aacgggaaat attttgttga cggcgtaagg gcttgggaag atttgtatat tgcgaccact
     8341 tcctccttaa tatcgatttc cgaaaacacc gatggtgtta aagtagctaa atatgttgca
     8401 caaggggcag aggccgtcac cagagccctt aacgaacaac tgggaacttg ttatcaataa
     8461 tgaaagatac ttatataaca aaaagtgaac gaacttttca aaatttaaat tattcacttt
     8521 attcggatgg tacttcgcat cctttgctgt ttttctttca cggttttacc agcaatcgtc
     8581 atttgggaat catggggcgg ggcgaagtgt tggcccgctt aggctttacc gtggtggcat
     8641 ttgatgctcc ttatcatgga gaccgcgata atgggacatt tagtcaatta tctgttagtg
     8701 ccaggcaaaa agaattaatg gaaattgtga ttcaaaatgc acatgaagct aagtggctgt
     8761 atgaaaagca tttacaagcg gaacaactaa ttagcaaagc tcccgttttt gcttttggtg
     8821 tgtccatggg ggcgcaagtt tgcttttatc ttgccacgat tatgcctaaa ctgcaaacaa
     8881 ttgttacgct ggtgggctca ccgtcatttt gtgattttta tcgttggaaa caacaacaat
     8941 accaatggcc cgatgacctt gaatttaaaa ttaagctgga aaaataccaa agtttggatc
     9001 ccttaattca tagcgaacga ttgcaaaaca ccaatatttt tatgggggtt ggcacccaag
     9061 atgatgttgt ccctaaacat tatgctaagc aactggctga gaagctgaag acaagtgttt
     9121 accaagagta tgaaacagcg catttttcaa cacccaagat gcttgatgat gcttattgtt
     9181 tccttaaaaa aacgctcact ttttaaaaga gcgtttttta ttttagtgta tttaaataat
     9241 taagaacttc gcttgctcca tggtaataag caacgacaat gtggttttta attaataata
     9301 gggtgggaac ggacgggatt tgtaaatcac ttaacttgga aacacccaag accgattcaa
     9361 cagggccaaa caaactggca ttgtgattat cttcatattt aatttgccag attggtaaaa
     9421 gcggttcatg attgcgggcg gtttgtgcat catgataacg atagatatca gccatgatta
     9481 attcacagcc gtaacaattg gctttggaaa agaaaactaa aaaacttgtt ggtttttcaa
     9541 acaattcttc ttttaagagc gaaggaatgt tttggttaaa gccgcttatt tctagtttta
     9601 cactgagagt gtttttcgaa tcagtgatgt ttattcggta ttcgccagca ataaaacaat
     9661 taagggtttc acttcgaata attaattgtg cgtcttcgta tgtccaatca agtgtataaa
     9721 catcgttttc gtcaagtaaa acaacttcct caggagtaaa gcaaagcgcg atatttaatt
     9781 gatgggggtt ttgtttatca tattgataaa gaggatcatc ttggcatcct gccaagatgg
     9841 ttaatattaa caagaggagg atttttttca ttttatcacc gctattatta tatcaaaaca
     9901 aagaacaatt gaccattgtt tcataaatat gttaaaatac ggcaaaggtg agttatgaaa
     9961 aaacatttat ggactatttt actaatcatt tggataagtt ttattttcct taattccttc
    10021 caaacaggcg cacagtcttc ccaagccagc ggcattttta ccaacttttt cacaaacctg
    10081 tttttaaaaa tcggttggtc cgttaatcag gaaacgctgt cgatgttaat ccgtaaaggt
    10141 gctcatatgt ttgagttttt tgtgttagca cttttatttt gtttgcaata ttttcggaaa
    10201 catccgcttc aaaaaacctt ggaaaaagcc ttgattagtt caatagtcgt tgctgtcgtt
    10261 gatgaattga ttcaactgag cgttcccgga cgcgttggca ccataatcga tgtggcaatt
    10321 gataatgttg gcatcgtgtt tgctttagtt ttgttttggc tcattgttcg tcataaacaa
    10381 aaaaaatccg ccaactcctc ttaacaacta aatccgtttg tgatataatg acaatagttt
    10441 tataaataaa ggagattttt actattgaaa aagcttaaca tttttctgat aacaatctta
    10501 ggatttttgc ttgttggttg caaagccacc gatattgatg ttttccgctt cgtcaatcat
    10561 gagattacaa ttcaagttgg cgacgatgcc gtgttaaatt tgatttatgg cgaatatgat
    10621 gccgatgatg aagttactta tactctttca aaagaaggaa ttattgtttt gttcagaaac
    10681 accgccaccg ggatagctcc cggagaagtc acggtaacag cgacgattga tggaattaag
    10741 aagacaaaca tcattgtgac ggttatcaac gaacccatca actccatgag tataagttcg
    10801 gcggcgtttg tgactgttgg cacccccatt caattagcgg tttcggtttt accaagtcat
    10861 ctgtctaatg aagtgacatg gtcaatcgag gatagtgtgc aaagcgatgt cgaggcggca
    10921 actgtttcac ccagcggttt attgtcgggt gtgattggtg ctaccacgaa agcggaactg
    10981 acggcgggag gaaaacagat tgttgttgtg gcgacatcga aagttgatcc cttgatgagt
    11041 gtgaaaaaga ccattcttgt taagtatcaa ccaacaacca aagtcacttt aagtaccgcc
    11101 ggaggtaaaa cgacgatggg agttacggat actttacagt tagttgccac cattttaccg
    11161 gcccatgctt gtccggtttt aacttacact tcaagtaata catccgttgt tacggttagt
    11221 aatacgggtt tagttagtgt tcctgaaaac agtaccaatt acaaaacgac aacgattacg
    11281 gccaccagtc ttgacaatgt ggtgggaacc attgatatta ccgttttaga ccctaatgct
    11341 taaggaggag gaagaaaatg aaatattgtg ctaattgtgg caaccaacta gaggatgaag
    11401 cggcgttctg tccgcagtgt ggaactaggc aggatggaaa gtttgcggcg aaaacggcaa
    11461 acaatgatgc gcccagcgtt ggttatgcta ttttaagttt tttgataccg cttgttggct
    11521 taatcctttt tttaaattgg aaaaataatt ttcctaggcg tgcaaaatct tgtgggatag
    11581 ccgctttaat cggtttcatt attaattttg ttgtcttttt ttatttacca taatgtcttt
    11641 ttgattgatg ttatttggtg atttcttttt taataggcat aaaaaaaccg gatggctccg
    11701 gttttttgtt agcgatattt tcttcggaag atgaaaacca aaccgataat tgaaatggtc
    11761 caaaagaggc tggttgcttg gaagcaacct tttttctttg gttcgggtt
//
LOCUS       DAOSGN010000003         9578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_6073_length_9578_cov_3.553292, whole genome shotgun sequence.
ACCESSION   DAOSGN010000003 DAOSGN010000000
VERSION     DAOSGN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6073_length_9578_cov_3.553292"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PK087_00130"
     CDS             complement(<1..595)
                     /locus_tag="PK087_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17705.1"
                     /translation="MNKSKVILTIFIIIISFFLISCDEVTSPFAVISIEKVGQNLEGY
                     IYEIKFVDNNSYQFIVPFGTDGENGLTPRIGTNGNWWIGEEDTGIFAQGPQGEQGNSG
                     VNGENGLTPRIGANGNWWIGEEDTGISAQGPQGEQGNSGVNGENGLTPRIGANGNWWI
                     GTVDTGVKAQGSSGTDGKDGITPHIGENGHWWIGDTDT"
     gene            complement(588..1427)
                     /locus_tag="PK087_00135"
     CDS             complement(588..1427)
                     /locus_tag="PK087_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17706.1"
                     /translation="MDNKYREILNELYEIYEKINAYQEEALKLQNQIGERTNSISEQL
                     DLQTTNLSEFKEKILGYINEFSNKLNDVSNKISTDFQNTTQKRIDTIKLELSSEVGLL
                     LKSISKTFAEVYGNLNKDRIELINKLNENLELNKNEGNVVKDITTQLEEQNKYLAIIT
                     KESKNNISKHLDETKYIKEELELLKQNFELKYVYNKKRIIFTSIFSLIMIIMISSAIF
                     ASIYMIAYYLKLIFFPNRIYTLVIGILLLVLSIGLIAILALVIFNPPTFLKKNKQRKG
                     KNE"
     gene            complement(1420..1788)
                     /locus_tag="PK087_00140"
     CDS             complement(1420..1788)
                     /locus_tag="PK087_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17707.1"
                     /translation="MLNDCIRIRNICEKRREFLENRTKWDDDYKIMLHEDFILLDSYD
                     MTMTVLNDLVNDNKIDYINVARYIFGFTSSYKKIKQSLAKVDYYNGKVFIKVVNKELD
                     NVLGQKTKNHINKLKEIFGG"
     gene            complement(1857..4241)
                     /locus_tag="PK087_00145"
     CDS             complement(1857..4241)
                     /locus_tag="PK087_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH17708.1"
                     /translation="MALVYVTEQFIKAFNELDPEDKLQINSIVNDISVRNSREVPEYL
                     ARLYLGSGKAKWAGINKVLYHLYPQGRDSQHRLFYCFGGDLQPSTNKVDSDDVIFIAY
                     VTRHDDERKAAKRYNKSTITLFYFFDYSFEKENDVVSTPVFKIKLTNLQNEILLLTKP
                     PASIKGSAGTGKTLISYELFKNWIRQDNDSKFLYLTYTSKLINKAKDTLKFDGLDINK
                     DNTHMFQFKDLFIQLEIDRQIKTDQIIDELNAREKIDDIINKFRNFNPSFKDDIIFSN
                     YFVYTYIRGLMKGRVSEPKKIFNLNIKGLEMFLKDLNLEEEGFKIKKFLNDHLQKQLL
                     EEKDLTDFLDRIVSSKKKKNIDSILSKFDKKTIDKINSFRLINKEFNFIEDSNVEKEL
                     KKDGISDKKILDLIKLKQEYNKILKTYNLYDDNDCAKAILNYEIENSQKYDGIVIDEV
                     QDLTEIQIMAIAKLLKKGSNNIYFFGDPNQTINPTIYHFGRFNSYLYEDEEQNKRNIN
                     QIILKKTHRCGPNLLDYINHLVKLRKDFKLTTNTEDLIKEESERIGEFDGHFACLIEK
                     KELIDFVLITLKDATDYYLIVDNEKSKEKVIEGIKALEGDSYDNNIENQIITVQNSKG
                     LESESVVIFNLISDNIDIFNNLINETNNKVSSMTFNKFYVSVTRAKNSIIICEEGLGD
                     HEQVKQILFYSGEKKIPEDIVESDIEEYLRTTNEPGKFFDQAIQLIEEKEYEKASRKN
                     DTALRHLLNQFDTNDNLDKLKTEFKNSNLDEIQKLTSSISKENLEKYQHFFPTL"
     gene            complement(4590..5810)
                     /locus_tag="PK087_00150"
     CDS             complement(4590..5810)
                     /locus_tag="PK087_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH17709.1"
                     /translation="MLIRKQYLEKIRRFYESNLIKVLTGVRRCGKSVLLNQIKDELIN
                     ENGIEENHIISINFEDVQFAHISTFNQLNDFILTQIKDEGKYYIFLDEIQYVKEFEKT
                     LASLKATKNVSLFVTGSNSKLLSGRLASLLVGRCKEFKIMPFTYSELMEYYQEKGLEM
                     PNKPLQDFIKFGGMPQRLDYSLEEDIKEYLKALYYGIIDKDICNDKSKIDKETFITIS
                     KYIISNTSKEFSANRIVDYYNKNNSSKIYRENVYRYLEKLEQACLISRVKRYDIAAKR
                     TLKSIEKQFVVDNGFLLACSDSNQIFVSHALENIIHNELLFRGYDIRIGKTYKGEIDF
                     VAIKDGKKCFIQVAYLLSSDDVIEREFGAFASVRDSSPKYVLSLDDYDMSREGITHLN
                     IEDWLLNKVDITLS"
     gene            complement(6045..7766)
                     /locus_tag="PK087_00155"
     CDS             complement(6045..7766)
                     /locus_tag="PK087_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH17710.1"
                     /translation="MIRRFIKYYRPHWRLLVLDLFCALAVAGIDLLFPVATNRILREL
                     IPNSQIRTMLLVGFFLLVLYGIRFLFSYIIGYYGHVMGIRIETDMRSDLFRKLQVLDY
                     QFFDNKKTGELMTNLTSHLHDVSEMSHHAPEDLFISSLMLMGSFTILMFIEPLLTLIV
                     FVFLLMLVTYSLTRRRKMLNSFRVSRTVQGELNAEIESSISGIRLTKAYNNESYEQTK
                     FEKVNSQYRKARSNIFREIGLYGSGNDFFANLANLAVLIFGGYFVYKNRIDYIDLTTY
                     FLYVNFLIRPIQRLTNSMEQLQQGFSGIEKFYQIMDVEPKIVSKPKALKKSDFIGDIQ
                     FKNVEFSYSQDEHEFVLKNFSYEIPAGRKVAIVGETGVGKTTISKLLPRFYDVLKGEI
                     KIDGINVKDYDLYDLRSAIGIIQQDVFIFWGTIKDNILYGRPDATFEEVVEAAKKAQI
                     HDFIMSLEQGYDSLTGERGVKLSGGQQQRISIARLFLKNPKILILDEATSSLDNITEN
                     LIQQSFEELAKDKTTIMIAHRLSTIQNADEIIVLGKNGIMERGTHEELVQSGGYYQAL
                     YNAVVTI"
     gene            complement(7769..>9578)
                     /locus_tag="PK087_00160"
     CDS             complement(7769..>9578)
                     /locus_tag="PK087_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH17711.1"
                     /translation="DSQTALAIMEILKSISQKRLVILVTHNISLAQNYATRIIELLDG
                     KIQKDSHPVKERINNEKRLKKTPKLGFFDTLRLSIKNMLRTKTRTLLSMIAGSIGIVG
                     IGLVLSISKGVNSYIEEVQKSALGNYPITIMSQAKKKDEEGLEVVERESFPDGEFVSI
                     REGENRYDYYNVMDEDFLAYMETLPPQLYTVIDYNTSIAMNILSQTATGYRKVSTSQL
                     SEMSKDQNFVLAQYDVLKGSIPNAADEVAIIIDKYNCLDALTLYYLGIDYEGMESLSF
                     DQLLSKQFKLLFNNDLYVKQGDVFKTKGLSQYESLYNNSQFSIKVTAIMREKKNANTV
                     LYRSGLLYTPELTQVVYEDARQSNIYTEQLNYGLTKNVFTGQAYEDEVSFSSTSSKEY
                     QLKQTYLNIGAYVNTNRIYVYTPTFADRLAISNFVSLYDDENGQVKITYYDYMNNVTT
                     EFASLVKVFSTVLIIFSSVSLIVSAIMIGIITYVSIFERIRDIGILRSIGARKWDIAT
                     LFNFETLSIGFFSGLLGVLGVLVLVEPVNDFVKKMVIEYTVSFSGISQVIVAQFESRY
                     IFLMVFGSMLVSFMAGLIPAVIASGKNPIEALKTER"
BASE COUNT         3097 a         1741 c         1272 g         3468 t
ORIGIN      
        1 ctgtgtcagt atctcctatc caccagtggc cattttctcc aatgtgagga gttataccat
       61 ctttgccatc agttccagaa gacccttgag cttttacacc agtatcaact gtccctatcc
      121 accaattacc gtttgcacca attcggggag ttagtccgtt ttctccattc actcctgaat
      181 tgccctgttc tccttgtgga ccttgtgcgg aaatgcccgt atcttcttcc cctatccacc
      241 aattaccgtt tgcaccaatt cggggagtta gtccgttttc tccattcact cctgaattgc
      301 cctgttctcc ttgtggacct tgtgcaaaaa tacccgtgtc ttcttcccct atccaccaat
      361 taccgtttgt gccaattcgg ggcgttaatc cgttttctcc atcagtccca aaaggaacaa
      421 taaactgata tgagttatta tcaacaaatt taatttcata aatatagcct tcaaggtttt
      481 gaccaacttt ttcaatggaa atcacagcaa aaggagatgt gacttcatca catgatataa
      541 gaaaaaaaga aattattatt ataaatatag ttaagattac ttttgattta ttcatttttg
      601 cccttccttt gtttgttttt tttcaaaaat gttggtggat taaagattac taaagctaat
      661 atagcgatta aaccaatact caaaaccaat aataatattc caataactaa tgtgtatatt
      721 ctgtttggaa agaaaattaa ctttaaataa taagctatca tatagataga agcaaaaata
      781 gctgatgata tcattattat cattattaaa gaaaaaatag aagtaaatat aatccttttt
      841 ttgttataga catattttag ttcaaaattt tgtttcaata attctaattc ttctttgatg
      901 tattttgttt catccaaatg ttttgatata ttgtttttgg attctttggt aataatggct
      961 aaatatttat tttgctcttc tagttgtgta gtaatatctt taactacatt cccctcgttt
     1021 ttatttaatt caagattttc atttaattta tttattaatt caattctatc tttgtttaaa
     1081 tttccataaa cttcagcaaa ggttttacta atcgatttta acaacaaacc tacctcgcta
     1141 gatagctcta atttaatagt atcaatcctt ttttgagtag tgttttgaaa atcagttgag
     1201 attttgttgg aaacatcatt tagtttattg ctaaactcat taatgtatcc taatatcttt
     1261 tctttaaatt ctgaaagatt cgtagtttga agatccaatt gttcactaat tgagttggtt
     1321 ctttctccaa tttgattttg aagttttaga gcttcttctt ggtaagcatt gattttttcg
     1381 taaatctcat ataattcatt taatatttct cgatacttat tatccaccaa aaatctcctt
     1441 taatttgttt atatggtttt ttgttttttg cccaagcaca ttatctaatt ccttattaac
     1501 aacttttata aatacttttc cattataata atcgactttg gctagtgatt gtttgatttt
     1561 tttataagaa gaggtaaaac caaatatata tcttgctaca tttatataat caattttatt
     1621 atcattaact aaatcgttta aaaccgtcat cgtcatatca tagctgtcca ataaaataaa
     1681 atcttcgtgt aacattattt tgtaatcatc atcccacttt gttctattct ccaaaaattc
     1741 ccttcttttt tcacaaatat ttcgaattct aatacaatca tttaacatat ctaatctttc
     1801 gattagcagt tcttttgttt cttcatcaat ttcccaaaaa tctttttcaa catcaactac
     1861 aaagttggaa aaaaatgttg atatttttct aaattttcct ttgatatact tgaagttaat
     1921 ttttgtattt catctaagtt tgaattttta aattccgttt tcaatttgtc aagattatca
     1981 tttgtatcaa attgattcaa caaatgtctt aatgcggtat cattttttct acttgctttc
     2041 tcatattctt tttcttcaat taattggatt gcttgatcaa aaaactttcc cggttcattt
     2101 gttgttctta aatattcttc aatatcgctt tcaacaatgt cttctggaat tttcttctca
     2161 ccagaataaa ataaaatttg ttttacttgt tcgtgatctc ctaagccctc ttcacaaata
     2221 attatgctgt tttttgctct tgtaacagaa acataaaatt tgttaaaggt catagaagac
     2281 actttgttat tagtttcgtt tattaaatta ttaaatatat caatattatc agaaattaaa
     2341 ttaaatatta caacgctttc actttccaaa ccttttgaat tttgaacagt aataatttgg
     2401 ttttctatat tgttatcata agaatcacct tctaaagctt ttattccttc tattactttt
     2461 tctttagatt tttcattatc aacgattaaa taataatctg ttgcatcttt taaagtaatc
     2521 aaaacaaaat caattaattc ctttttttct attaaacatg caaaatgtcc atcaaattct
     2581 cctattcttt ccgattcttc ttttataaga tcttctgtat tagtagttaa tttaaaatcc
     2641 tttcttaatt taaccaaatg atttatgtaa tctaacaaat ttggaccaca cctgtgtgtc
     2701 ttttttaaaa ttatctgatt gatatttcgc ttgttttgtt cttcatcttc ataaagataa
     2761 gaattaaatc gaccaaaatg ataaattgtt ggattaatag tttgattggg atctccaaaa
     2821 aaataaatgt tgttgcttcc tttttttaac aacttcgcta tggccattat ttggatttct
     2881 gtcaaatctt gaacttcgtc aatgacaatt ccatcatact tttgggaatt ttctatttca
     2941 taatttaaaa tggcttttgc acaatcgtta tcatcatata aattataagt ttttaggatt
     3001 ttattatatt cttgttttag cttaataaga tccaagattt ttttatcgct aattccatct
     3061 ttttttaatt ctttttcaac attgctgtct tcaataaaat tgaactcttt attaattaat
     3121 ctaaaggaat ttattttatc aattgttttt ttatcaaatt tagacagtat ggaatcgatg
     3181 ttttttttct ttttacttga aactatccta tctaaaaaat cggttaaatc tttttcctca
     3241 agtaattgtt tttgtaaatg atcatttaaa aattttttta ttttaaaccc ttcttcttcc
     3301 aaatttaagt cttttaaaaa catttcaagt ccttttatat ttaaattgaa aattttttta
     3361 ggctcggaaa ctcttccttt cattagtcct ctaatataag tgtaaacaaa ataatttgaa
     3421 aaaataatat cgtctttaaa agagggatta aaattacgaa atttattaat aatatcatct
     3481 atcttttctc ttgcatttaa ttcatcaatt atttggtcag tttttatttg tctatcgatt
     3541 tccaactgaa taaataaatc tttaaattga aacatatgag tgttgtcttt attaatatct
     3601 aatccatcaa attttaatgt gtcttttgct ttattaatta atttggatgt ataagtcaaa
     3661 tacaaaaact tactgtcgtt atcttgtctt atccagtttt taaataattc ataactaatt
     3721 aatgtttttc ctgtcccagc actacctttg atagaagctg gtggtttagt taacaataaa
     3781 atttcatttt gcaagtttgt aagttttatt ttaaaaacag gtgtagatac aacatcattt
     3841 tctttttcaa atgaataatc aaaaaaataa aataaagtaa ttgttgattt attatatctt
     3901 tttgctgctt ttctttcatc atcgtgtctt gtaacataag caataaaaat tacatcatcc
     3961 gaatcaactt tatttgttga aggttgaaga tcccccccaa aacaataaaa cagtctatgt
     4021 tggctgtctc ttccttgagg atataagtga tacaagactt tattaattcc cgcccacttt
     4081 gctttcccac tacctaaata taagcgtgct agatattctg gcacctctcg ggaattacga
     4141 acacttatat cattcactat agagttaatt tgtaacttgt cctcaggatc tagttcatta
     4201 aatgccttta taaattgttc ggtaacatat actagtgcca ttagtcaatc tccaataata
     4261 attaatttct tattagtatt gtaacaatta ttaaaaacaa tgtaaataat ttataccttt
     4321 tccatttaat tatttaaatt aaaaacacct taattcttaa aaccaaggtg ttctattttt
     4381 tttatttggt atttcgaaga gaattcaagt ctttaataaa tgacttagaa gaacattcgc
     4441 tgtcaataaa aagttcttat aataaaacaa aagcaattac ttcagcaatc caattgttta
     4501 atctaagtaa catagttaaa aggctgataa tctcaatatt ttataatcta agttattaca
     4561 aatcaaaaat aaatacgttt ttgtaacact tatgataagg taatatcaac cttgtttaac
     4621 aaccaatctt caatgtttaa atgcgtaatt ccctccctag acatatcata atcatctaaa
     4681 cttaatacat attttggcga agaatcacga actgaagcaa atgcgccaaa ctctctttca
     4741 ataacatcat cactagataa tagatatgca acttgaataa aacatttttt cccgtccttt
     4801 atcgctacaa aatcaatttc acctttatat gtttttccaa tcctaatgtc ataacctcta
     4861 aataacaatt cgttatgaat gatgttttcc agcgcatgag aaacaaagat ttgattggaa
     4921 tcactacaag caagcaaaaa gccattgtca acaacaaact gtttttcaat tgattttaat
     4981 gttcttttgg cagcaatatc ataccgtttc actctcgata tcaaacaagc ttgttcaagt
     5041 ttctctaaat aacgataaac attttcgcga tatattttac tggagttgtt tttattataa
     5101 taatcaacaa tcctgttggc agaaaattcc ttagaagtat tactaatgat atattttgat
     5161 atggtaataa aagtttcttt atcaatttta gatttgtcat tacaaatatc tttatcgatt
     5221 attccataat aaagcgcctt cagatattct ttaatatctt cttctaatga ataatccaaa
     5281 cgctgaggca taccaccaaa tttaataaag tcctgtaagg gcttattggg catttctaag
     5341 cctttttctt gatagtattc catcagttca ctgtatgtaa agggcattat tttaaattct
     5401 ttacatcttc caaccaataa actagcaagt cgtccggata aaagttttga attgcttcct
     5461 gtcacaaaaa gagaaacatt ctttgttgct ttaagagacg ctaaggtttt ttcaaattct
     5521 ttcacatatt gaatttcatc caagaagata taatatttac cctcatcttt aatttgtgtc
     5581 aaaataaaat cattaagttg attgaatgtg ctgatatgtg caaattggac atcttcaaaa
     5641 ttaattgaaa taatgtgatt ttcctcaata ccattttcat taattaattc atctttgatt
     5701 tgattaagta acacactttt tccacatctt ctaacgcctg ttaatacttt aattaaattg
     5761 gattcataaa atcttcttat tttttctaaa tattgttttc taataagcat ctatactcac
     5821 cccaaagata ttatatcata gaataaaaca atgtgttaca aatttaaaat aattttaagt
     5881 ttgtaacaca tattgatata ttaatctttg gaatctttaa tcaaaagatt atcctaataa
     5941 attcccatat ctaccataaa taaaaaaaca ccctaattta taaaactaag gtgtttttag
     6001 ctaagttagg tttttgttaa taactaacaa aatgcttaac tcgtttaaat cgtcacaact
     6061 gcattataaa gagcctgata ataaccaccg ctttgtacaa gctcctcatg tgtccctcgt
     6121 tccataatcc cgtttttccc taggacgatg atttcatccg cattttggat tgtggaaaga
     6181 cggtgggcaa tcataatcgt cgttttgtct ttcgccaatt cctcaaacga ttgttggatg
     6241 aggttttcag taatgttatc cagtgaagaa gtcgcttcat ccaaaatgag aatcttcggg
     6301 tttttcaaaa acaaccgggc aatcgaaatc cgttgttgct gacccccgga taacttcact
     6361 ccccgttcac cggttaagct gtcataccct tgttcaagcg acataataaa gtcatggatt
     6421 tgggcttttt tagccgcctc gacaacttcc tcaaaagtcg catccggacg accatataag
     6481 atattatctt taatcgttcc ccagaaaata aagacatctt gttggatgat gccgatggca
     6541 ctacgcaaat catacaaatc ataatctttc acattaatgc cgtcaatctt aatttcccct
     6601 tttaaaacat cataaaagcg gggaagcagt ttggaaatcg ttgtcttccc gactccggtt
     6661 tccccaacaa tggcaacctt ccttccggcc ggaatctcat aactgaaatt ctttaaaaca
     6721 aattcatgtt catcctgact atagctgaat tccacatttt taaattgaat atcgccgatg
     6781 aagtcgcttt ttttaagggc cttaggttta gaaacgattt tcggttccac atccataatt
     6841 tggtaaaact tttcaattcc ggaaaaaccc tgttgcagtt gttccatgga attagtcaag
     6901 cgttgaatcg ggcgaattaa aaagttaaca tacagaaaat aagttgttag atcgatataa
     6961 tcaatccggt ttttataaac aaagtagcca ccgaaaatta aaacggctaa attggctagg
     7021 tttgcgaaaa aatcattccc ggaaccgtat aaaccaattt cccgaaaaat attggagcgg
     7081 gctttccgat actgcgaatt gactttttca aactttgttt gttcataact ttcattattg
     7141 taagctttcg tcagccgaat tccgctaata ctgctttcaa tttccgcatt caactcccct
     7201 tgaacggtcc gagaaacacg aaaagaatta agcattttgc gtcttcgggt aagcgaataa
     7261 gtaactaaca tcaataaaaa gacaaatacg atgagcgtta ataagggttc aataaacatg
     7321 agaatggtga aactgcccat taacataaga ctcgaaataa acaaatcttc cggagcatga
     7381 tgactcattt ccgaaacatc gtgaagatgt gaggtgaggt tggtcattaa ttcaccggtt
     7441 tttttattat caaaaaactg ataatccaaa acttgcaatt ttcgaaacaa atcactgcgc
     7501 atatcggttt caattctaat ccccatcaca tgaccgtagt aaccgatgat atacgaaaac
     7561 aaaaacctga tgccatacaa aaccaacaag aaaaagccga ccaagagcat agtcctaatt
     7621 tgggaattag ggattaattc tcttaaaatg cggttggtgg ccacggggaa aagtaaatca
     7681 attcccgcca cggctaacgc acaaaataaa tccaaaacta aaagccgcca atggggccga
     7741 taatatttaa taaatcttct aatcatttct acctttccgt ctttaaggcc tcaatcggat
     7801 ttttaccgga ggcaatcact gccggaatta aaccggccat aaaagaaact aacatactgc
     7861 caaaaaccat taaaaagata taacgcgact caaactgcgc cacaatcacc tgcgaaatac
     7921 cgctaaaaga aaccgtatat tcaataacca ttttctttac aaaatcatta accggttcaa
     7981 ctaaaaccaa gacccctaaa acaccaagga gcccggaaaa gaaaccgatg cttaaagttt
     8041 caaagttaaa caaagtagca atatcccatt tacgagcgcc aatgcttcgc aaaataccaa
     8101 tgtccctaat tctttcaaaa atggaaacat aagtaataat cccaatcata atagccgaaa
     8161 caattaagga caccgatgaa aaaataatta aaaccgtact gaacaccttc acaagggaag
     8221 caaattccgt cgtcacattg ttcatataat catagtaagt gattttaacc tgcccatttt
     8281 catcatcata gaggctaaca aaattactga tggctaaacg atccgcaaag gtcggtgtat
     8341 aaacataaat cctattggtg ttaacataag cgccaatgtt aagataagtt tgcttcagtt
     8401 gatattcttt ggagcttgtg gaagaaaagg aaacttcatc ttcataagct tgacccgtaa
     8461 aaacattttt tgtaaggcca tagttaagtt gttcggtgta aatatttgat tgtctggcat
     8521 cctcataaac cacttgcgtt aactcaggcg tgtacaaaag accgctacga tataaaacgg
     8581 tattggcgtt tttcttttcc cgcataatgg cggtgacttt aattgaaaat tgtgaattgt
     8641 tatataagga ttcatattga cttaaaccct tagttttaaa gacatcccct tgtttaacat
     8701 ataaatcgtt attaaaaagc aatttaaatt gtttacttag caattgatca aaacttagac
     8761 tttccatccc ttcataatca attcctaaat aataaagtgt taaagcatcc aaacaattat
     8821 atttatcaat gataatggcg acttcatcgg ccgcattagg gatgcttcct tttaaaacat
     8881 cgtactgcgc cagcacaaag ttttggtcct tgctcatttc acttagttgg ctggtgctta
     8941 ctttgcgata acccgtggct gtttgtgata aaatgttcat cgcaatgctg gtgttataat
     9001 caatcaccgt ataaagttgg gggggaagag tttccatata ggccaagaaa tcttcatcca
     9061 tcacattata ataatcatag cgattttcgc cttccctaat cgaaacaaat tcaccgtccg
     9121 ggaagctttc ccgctccaca acttcaaggc cttcttcatc tttcttcttg gcttgactca
     9181 tgatggtaat cggataattg cctaaagcag atttctgcac ttcttcaata taagaattga
     9241 cccctttgga aatcgatagc accaatccaa tcccgacaat cccgatacta cccgcaatca
     9301 tggaaagcaa ggtgcgggtt ttcgtgcgca acatgttttt aatgcttaag cgtaaagtat
     9361 caaaaaaacc taacttgggt gtttttttaa gccttttttc gttattaatt ctttccttaa
     9421 ccggatggct gtccttttgg attttgccgt ccagcagttc gataatcctc gtggcgtagt
     9481 tttgagctaa actaatattg tgagtaacta aaataactaa gcgtttttgg gaaatgcttt
     9541 ttaaaatttc cataatggcc agggctgttt gcgaatcc
//
LOCUS       DAOSGN010000004         8529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_7102_length_8529_cov_3.469554, whole genome shotgun sequence.
ACCESSION   DAOSGN010000004 DAOSGN010000000
VERSION     DAOSGN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7102_length_8529_cov_3.469554"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PK087_00165"
     CDS             complement(<1..541)
                     /locus_tag="PK087_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOH17712.1"
                     /translation="MNKQLLSRWLLLVMLLLLGLTAVGCQKDNDDNDDDEIITVDSLT
                     TEYTDKLKLTASYKNKDFLADGIGEVTLLRVVDGDTAHFLNKKGDTIKIRFLNINTPE
                     STGVIAAWGKKASSYTKIKLQAATEIVLESEVLDQAAQLDSTGGRYLGYVWYRLGAND
                     DLRLLNLEIIEQCFSYFTGD"
     gene            complement(560..952)
                     /locus_tag="PK087_00170"
     CDS             complement(560..952)
                     /locus_tag="PK087_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH17713.1"
                     /translation="MLNQNSDEEKLAAWLFIKFLMEPEKSAQFAIATGGYFPVRKSAY
                     ETAKYTEYLTNPTDDKVHYSKAANVVLNDYIEQEYIYFVDDAFVGSAAIRNEAGAIFN
                     DIMVNNKVIEARINEAYKTLKPYVEAGK"
     gene            complement(945..2045)
                     /locus_tag="PK087_00175"
     CDS             complement(945..2045)
                     /locus_tag="PK087_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH17714.1"
                     /translation="MKKSLILLVLLFSVFMFVGCKQNVRVTDNFKVLSVEEIANKDIT
                     IKFVVRSGVISTALVDLVTEFKERYPKITVDVEALSGSYDLIRSTTMIDINSKQAPDL
                     VIGYPDHFAEYYSAGALVNLTRFIEDKKVGFTEEEMSDFLETYLKENRGFNDEKPEYI
                     YGLPFNKSTEVLVYNKTFFEKLYGETWQIKIPKTWQELETVGADIVTRVRNKEADNIF
                     VQSIDPKTQVKTYLKVSDYLATGKFIPLGYDSADNAFITLTRQFGAEYTKRIDVEHGY
                     VRFNNQQSKDAMAYFKGLKDNGIFGTSALYGLDYMSDAFKLIQSVMSVGSSAGVGYNA
                     SAGFAYELGVAPIPYYSAEKICHPTRNEHCDA"
     gene            complement(2094..3083)
                     /locus_tag="PK087_00180"
     CDS             complement(2094..3083)
                     /locus_tag="PK087_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH17715.1"
                     /translation="MERELYLDKEALRTFKIKRAIGNIFQYGFLILMALFIVIPFYWM
                     LISSVKETWEIISPELTLLPHNIETRKIAWPWDWAWENYAKLIVGIPNTSGVGGEWTF
                     RPMPFGWMFLNTIVVGLFTTIGMVFTVVLAAFAFSRLNFKGRDVLFTIMLATMMIPGE
                     VFVITNYMTISQWGWVRTDNHFHNYLALILPFITSVFYTFFLRQTFKQIPNELYLAAK
                     VDGTTDFKYLLRVMIPMAKATLTTIIILSMIGTWNAYIWPSFVTNAANRHHLLVSNGL
                     IVGLAGAVESTGPNSILNLQMAASTLVTVPLLIVFFVFKKHIMRGVSRSGIKG"
     gene            complement(3094..4029)
                     /locus_tag="PK087_00185"
     CDS             complement(3094..4029)
                     /locus_tag="PK087_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH17716.1"
                     /translation="MLEYKKDGWKATLALAPMLLLMSVFTFWPIINSLIMAFLEDYSF
                     GGMASHAYARVIGGGTYNGIGFANFVQVFNEKDFWVALKNTSVMVFISVPLTVIIGLA
                     IAVGLNATKKLQGFFQTVFFLPYVTNTIALGLVFGSVFHIEYGLINKVFGTVGLSWIN
                     HGATWWRAMLVLMVYTVWNGLAFKIIVFLSGMQSIDKQYYQAAQIDSTPRWRVFSKIT
                     VPLLSPMILYITITSFIGAFKAYSSVIAIFGTGDYGPVGNEKMMITLVGYIYDKMWES
                     GTPFGVGSAGSLILFAIILLITFVQFRVSKKRVHY"
     gene            complement(4029..5147)
                     /locus_tag="PK087_00190"
     CDS             complement(4029..5147)
                     /locus_tag="PK087_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH17717.1"
                     /translation="MEKVSTNVTDVGDKIIHQSVDVRLQNLTKVFFEKSGKKTVAVDD
                     FSILIPAGKLIGLLGPSGCGKSTTLYMIAGLHKPTAGSIFFGDDDVTNMPPEKRGIGL
                     VFQNYALYPHFTVRQNIMFPLENLKVPKEEALKLAQQMAELVHIGDLMDRKPSQLSGG
                     QQQRVAIARALVKRPRVLLLDEPLSNLDARLRLQTREEIKRIQRETGITTIFVTHDQE
                     EAMSISDEIVVMNYGIKQQMNHPQAVYDEPVNMFVAKFLGNPPINIIPAKIVGGQLTL
                     LDNTVLEKGFEIADQEVMIGLRPEYFKIVSTGKITAQIEMIEHIGRDTMIICQLENAE
                     NTIRVIVPSAKNLTVNQKIKLNATKFFIFNKETGERYK"
     gene            complement(5353..5604)
                     /locus_tag="PK087_00195"
     CDS             complement(5353..5604)
                     /locus_tag="PK087_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17718.1"
                     /translation="MDFVKELFEKGDAKVGPILDPIVTKITEFLSNTGDTALALILLF
                     TGILILVGLFAWLRRTPRFFLFVLILFGIVVGAWFLFGK"
     gene            complement(5640..6236)
                     /gene="recR"
                     /locus_tag="PK087_00200"
     CDS             complement(5640..6236)
                     /gene="recR"
                     /locus_tag="PK087_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOH17719.1"
                     /translation="MKYPQALQKLIESFQLYPGIGPKTAERLALFTFMRLKEDDVIKF
                     SQHLLQVKKQIRYCENCGSLTEQAWCDICLDKNRLNQILVVETSKEVLSFEKTKQHRG
                     KYHVLNGLISPLKGIAPENINVSNLLKRIEEEKIDEVIIATNANIEGEMTALYLKKLL
                     APSNVLVSRIGYGLPAGGDVEYADEITLIKALEGRKEM"
     gene            complement(6238..6552)
                     /locus_tag="PK087_00205"
     CDS             complement(6238..6552)
                     /locus_tag="PK087_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOH17720.1"
                     /translation="MNQQALLKKVKKLQDEMMTTQKQIEQTSFYASAGGVVNVEVKGT
                     KELVKVSIEEDFEVEAPEDLEMLSDMVVAACNQAYKEIEKVTKEKMGVYNEVLGGFGG
                     FF"
     gene            complement(6562..>8529)
                     /locus_tag="PK087_00210"
     CDS             complement(6562..>8529)
                     /locus_tag="PK087_00210"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17721.1"
                     /translation="LDQAASFADDEITIDDVNSVTGNLNYDKTIELAEYLEAGNINQA
                     LATINDLLAAGKEISKIVSGILQFYRDLLLFKNVDTSTSSKYIYEKPAFRALAEKINE
                     AKIFYYVDTISDVQAKIKTSITPHIYLEVAIIKMINVGEQDLDFMKRFKVLEEEISSL
                     KQATPTNQDEAMVSNDKVNDIEYRLNRVVQELSKLELHKLSDRLGNLEKASLPSDSSQ
                     GMEEINRQLNQIQEQIRALNEVTTKQQAAMNQSLATSPAKNVDQDELREILQEELQGI
                     KQKITKLESINHNLPPNLPPSSLTEEWQTKINVLENKVYKLMSNVLAPVPKKTKKKTN
                     EGQIVLFGDEVTTLEALEKPTKIQIDFAELQKEEVKAPATTEAEEKETTEKQVEEAIL
                     ETVETEKEPLTKTAENLVLGELKVADFQEAKPKTVQTTILENQEIIDRPHSQLVRKIN
                     PEKPELEHMSVKDLEDNVKLTHMEKPEPITKPVVQEEQKPRLTIAKMMEKILHETRSE
                     AARHDKPRIMKLWDSLDKTTNADYRLIAELLQEGSVAAVGDKALVIVYPNAMLCNQVM
                     KPKFKKEALRMLYNYLGDAYDYLAIPDYLWKEKRSEYVNQYNIGTTYPVLSEIDDPSL
                     IFEDEDNEVRAIDKQIDQVKSLFGDDFVKVE"
BASE COUNT         2483 a         1825 c         1528 g         2693 t
ORIGIN      
        1 gatcgccggt aaaataagaa aaacattgtt caatgatttc taaattcaat aagcgtaaat
       61 catcattggc acctaagcgg taccacacat agcctaaata acgtccgccg gtggaatcaa
      121 gctgtgctgc ttgatccaag acttccgatt ctaaaacaat ctccgtggct gcttgtagtt
      181 ttattttggt atatgaactg gcttttttcc cccaagcggc aataacgccg gttgattccg
      241 gggtgttgat attcaaaaag cggattttaa tggtgtcacc cttcttattc aaaaagtgag
      301 cggtatcgcc gtcaacaacc cgaaggagcg tcacttcacc aatgccatca gcaagaaagt
      361 ctttgttttt ataactggct gttaatttca gtttatcggt atattcggtg gtcaggctat
      421 caaccgtaat gatttcatca tcatcattgt cgtcattatc cttttgacaa cccaccgccg
      481 tcaaaccaag caacaacaac ataacaagca acagccatct tgacaataat tgtttgttca
      541 ttgttcacct gtgaaggttt tatttgccgg cttcaacata tggttttaat gttttataag
      601 cttcattaat tcgtgcttca atgactttat tattaaccat aatatcgtta aaaatggctc
      661 cggcttcatt tctaatggct gctgagccga cgaaagcatc gtcaacgaaa taaatatatt
      721 cctgttcaat ataatcattc aaaaccacat tggcagcttt agaataatga accttgtcat
      781 cggttggatt cgttaaatat tcagtatatt tagctgtttc ataagccgat ttgcggaccg
      841 ggaagtagcc accggtagca atcgcaaatt gcgcactctt ttccggttcc atcaagaatt
      901 taatgaacaa ccaggccgct aatttttctt catcactgtt ttggttaagc atcgcaatgt
      961 tcgttccttg ttggatgaca tattttttca gcggaataat aaggaatcgg agcaacacca
     1021 agttcatagg caaatccggc actcgcatta tagccaaccc cggcacttga accaacagac
     1081 atgacgcttt gaatcagttt aaaggcatcg gacatataat ccaaaccata tagtgcggaa
     1141 gttccaaaaa tgccgttatc ctttaagcct ttgaaataag ccatggcatc tttcgattgt
     1201 tggttgttga aacgaacata gccatgttcc acatcaattc ttttggtata ttcggcccca
     1261 aattgacgcg ttaaagtaat gaaggcattg tcggctgagt catatcctag cggaatgaac
     1321 ttccctgttg ccaaataatc cgatactttc aaataagttt taacttgcgt tttgggatca
     1381 attgattgta caaaaatatt atcagcttct ttgtttctga cacgagtgac gatatcagca
     1441 ccaaccgttt ccaattcctg ccatgttttc ggaatcttga tttgccatgt ctcaccatat
     1501 aatttttcaa agaaagtctt attataaact aatacttccg ttgatttgtt gaagggtaaa
     1561 ccataaatat attccggttt ttcatcatta aaaccacggt tttcttttaa gtaggtttcc
     1621 aaaaaatcgc tcatttcttc ttcggtaaag cccactttct tatcttcaat aaagcgcgtc
     1681 aagttgacca aggcaccggc ggaataatac tctgcaaaat ggtcgggata accaatcact
     1741 aaatccggcg cttgtttgga gttaatatca atcatggttg tggaacgaat taaatcataa
     1801 ctaccggata aagcttccac atcgacagtg attttgggat aacgctcttt aaattcagtt
     1861 accaaatcaa ctagggcggt ggaaataacc ccggaacgaa caacgaattt aatggtgatg
     1921 tctttgttgg caatttcttc aactgacaaa actttgaaat tatcggtcac tcttacgttc
     1981 tgtttacagc caacgaacat aaaaacacta aagagaagca ccaataaaat taaacttttt
     2041 ttcatttttt gtttcctttc ttttttatat aactaagcct aagcttgggt tatctaacct
     2101 ttaataccgc tacgggaaac cccgcgcata atgtgttttt taaatacaaa gaagacaatg
     2161 agcaaaggaa ccgtaaccag tgttgaggcg gccatctgca aattgagaat cgagttgggg
     2221 ccggttgatt caaccgctcc cgccaagccg acaattaagc cgttactaac caataaatga
     2281 tgacgattgg ccgcattcgt aacaaacgaa ggccaaatat aagcgttcca agtccctatc
     2341 atacttaaga taataatcgt tgttaatgtc gctttcgcca tcggaatcat cacgcgtaat
     2401 aaatatttaa aatccgtcgt tccatcaact ttggctgcca aatataattc attagggatt
     2461 tgtttgaagg tttgccttaa gaagaaggta tagaagacac tggtaataaa gggcaaaata
     2521 agcgctaagt aattatgaaa atgattatcc gtgcgcaccc agccccattg ggaaatggtc
     2581 atatagttgg taatcacaaa gacttccccc ggaatcatca tcgtggctaa catgattgtg
     2641 aaaagcacat cacgcccttt aaagtttaac ctagaaaaag caaaggccgc taaaacgacg
     2701 gtgaaaacca tgccaatggt ggtaaaaaga cctaccacaa tggtgtttaa aaacatccag
     2761 ccaaagggca tcggtcggaa tgtccattct ccgcccaccc cgctggtatt aggaatcccg
     2821 acaatcaatt tcgcataatt ctcccaagcc caatcccaag gccaagcaat cttccgcgtt
     2881 tctatattgt ggggtaaaag ggttaattcc ggcgaaataa tttcccatgt ttctttaacc
     2941 gaactaatga gcatccagta aaaaggaata acgatgaata aggccattaa aattaagaat
     3001 ccgtactgga agatattgcc aatggcccgc tttattttaa aggtccgcaa ggcttcttta
     3061 tcaagatata attctctttc cattgcctac ctcctaataa tgaacccgtt ttttgctcac
     3121 gcggaactga acaaaggtga tgagtaaaat gatggcaaat aaaataagtg aaccggctga
     3181 gccaacaccg aagggcgttc cgctttccca catcttatcg taaatataac cgactaaggt
     3241 aatcatcatt ttttcattgc cgaccggacc atagtccccg gttccgaaaa tagcaataac
     3301 ggatgaataa gctttaaaag cgccaataaa tgaagtaatc gtaatatata aaatcatggg
     3361 tgataataag ggaacggtga ttttggaaaa gacgcgccac cgcggagttg agtcaatttg
     3421 cgccgcctga taatattgtt tgtcgatgct ttgcatgccc gacaaaaaga caatgatttt
     3481 aaaggctaga ccgttccaaa cagtataaac cattaacacc agcatcgctc gccaccaagt
     3541 ggcaccatga ttaatccaag aaagaccgac tgtcccaaac actttattaa tcaagccgta
     3601 ttcaatatgg aaaacagagc cgaaaactaa accaagggca atcgtgtttg taacataggg
     3661 aaggaagaaa acggtttgga aaaatccttg taatttcttg gttgcgttta aaccaaccgc
     3721 aatcgctaaa ccgataatga cggttagagg cacggaaatg aacaccatca cactggtatt
     3781 cttaagggct acccaaaaat ctttttcatt aaacacttgc acaaagttgg caaaaccgat
     3841 gccattatat gtgcccccac caattactcg agcataggca tggctagcca ttccgccgaa
     3901 tgaataatct tccaagaagg ccataatcaa ggaattaata atcggccaga aagtaaacac
     3961 tgacataaga agaagcattg gcgccaatgc cagagttgct ttccaaccat ctttcttata
     4021 ttccagcatt atttatacct ctctcctgtt tctttattga agataaagaa tttcgtcgca
     4081 ttcaatttaa tcttttgatt gacagttaga tttttagcac taggaacaat cacccgaatg
     4141 gtgttttccg cattctccaa ttggcaaata atcatcgtat cccggccaat atgttcaatc
     4201 atctcaattt gagcggtgat tttgccggtg gaaacaattt tgaaatattc cggacgcaaa
     4261 ccaatcatga cttcttggtc agctatctca aaaccttttt ccaaaacagt gttgtcaagt
     4321 aaggttaatt gaccaccaac aattttagcc ggaataatgt taatgggagg atttcctaaa
     4381 aacttagcaa caaacatatt caccggttca tcataaacgg cttgcggatg attcatctgc
     4441 tgtttaatac catagttcat caccactatt tcatccgaaa tgctcatcgc ttcttcttgg
     4501 tcatgggtaa caaagatggt tgtgattccc gtttctcgtt gaattcgttt aatttcttca
     4561 cgcgtttgta agcgaaggcg ggcatcaagg ttggagagcg gttcatcaag taacagcact
     4621 cgcggtcttt ttacaagcgc acgggcaatc gccactcgct gttgttgacc accgcttaat
     4681 tggctgggtt tacgatccat taaatctcca atatgaacca gttccgccat ttgttgagcc
     4741 agctttaagg cttcttcttt aggaactttt aaattttcca acggaaacat aatgttttgg
     4801 cgaaccgtaa aatgaggata gagggcatag ttttgaaaaa ccaagccaat gcctcttttt
     4861 tccggcggca tgttggtaac atcatcatcc ccaaagaaaa tggatcccgc tgtcggttta
     4921 tgcagtccgg caatcatata aagcgtcgtt gacttaccac aacccgaagg tcctaataag
     4981 ccaattaatt ttccggccgg aataaggatt gaaaagtcat caaccgcaac ggttttttta
     5041 ccgctttttt caaaaaatac ttttgtaaga ttctgtaagc gtacatctac actttgatgg
     5101 atgattttat cgcctacatc cgtaacattc gtgcttactt tttccatttc caactccttt
     5161 aaatatctat ttacttgtct attttatcat gaaggccaaa taaagtaaaa ggttatttga
     5221 aaaaacgccc ttgttgggtg ttttttgtgc ttttttattg tctctttttc gaaatcaaat
     5281 ttttgattta tcaaatattt cctttaaaaa caaaaagaac cgccaagcag ttttctgcca
     5341 agcagttctc cattattttc caaataaaaa ccaagcgcca acaacgattc caaataaaat
     5401 aagaacaaat aagaaaaatc tcggcgttct cctaagccat gcaaacaatc caacgaggat
     5461 gaggatcccc gtaaataata atatcaaagc aagggcggta tcaccggtat ttgataagaa
     5521 ttcagtaatt ttcgtaacaa tcggatctaa aatcgggcca actttggcat cacctttttc
     5581 aaataattcc tttacaaagt ccattttttt ctcctcttaa gtagatttag ttaattatac
     5641 tacatttctt tccgaccttc aagtgctttt attaaagtaa tttcatcagc atattcaaca
     5701 tcaccaccgg caggaaggcc atagccgatg cgcgaaacca aaacattgct tggtgctaac
     5761 agttttttta agtataaagc cgtcatttcg ccttcaatat tggcattggt agcaataata
     5821 acttcgtcaa ttttttcttc ttcaatccgc tttaacaagt tcgatacatt aatattctcg
     5881 ggcgcaatcc cttttaaagg ggaaataagc ccattaagga catggtactt cccgcggtgt
     5941 tgcttcgttt tttcaaacga caatacttct ttactagttt caacaactaa aatttgattt
     6001 agacgatttt tatctaaaca aatatcacac caggcttgtt cggttaagga tccacaattc
     6061 tcacaataac gaatttgttt ttttacttgt aataggtgtt gggaaaactt gataacatcg
     6121 tcttccttta atctcataaa agtaaacaag gctaagcgtt ccgcggtttt gggtccaatt
     6181 ccgggataca attgaaagct ttcaattaat ttttgtaaag cttggggata tttcatgtta
     6241 gaaaaacccg ccaaagccac caagcacttc gttatataca cccatttttt ctttcgttac
     6301 tttttcgatt tctttatagg cttgattaca agcggcgaca accatatcgc ttaacatttc
     6361 taaatcttcc ggggcttcaa cttcaaagtc ttcctcaatg ctcactttca caagttcttt
     6421 cgtccctttg acttctacat taacaacacc gcccgccgaa gcatagaacg atgtttgttc
     6481 aatttgtttt tgcgtggtca tcatttcgtc ttgtaacttt ttgacttttt ttaataacgc
     6541 ttgttgattc ataatatctc cttattctac ttttacaaaa tcatcgccaa acagcgattt
     6601 tacttggtca atttgtttgt cgattgctcg aacttcattg tcttcgtctt caaatattaa
     6661 actaggatca tcaatctcgg ataaaacggg ataagtcgtg ccgatattat attgattgac
     6721 atattcactg cgtttttctt tccaaagata atcgggaatc gctaagtaat cataggcatc
     6781 acccaagtaa ttatagagca tccgtaaggc ttctttttta aatttcggtt tcatcacctg
     6841 attgcaaagc atggcattcg gatacacaat gacgagggct ttatcgccga cagcagccac
     6901 tgagccttct tgtaataact cggcaattaa acggtaatcg gcattggtgg ttttatccag
     6961 tgaatcccat aatttcatga tgcgcggttt gtcatggcga gctgcttcac tacgagtttc
     7021 atgtaaaatc ttctccatca ttttcgcaat cgtcaagcgc ggtttttgtt cttcctgaac
     7081 caccggttta gtaatcggtt ccggtttttc catatgggta agtttgacat tatcctctaa
     7141 atccttgaca ctcatatgct caagttccgg tttctcggga ttgattttcc gaaccaattg
     7201 cgaatgaggt cgatcaatga tttcttgatt ttcaagaatt gttgtttgca ccgtctttgg
     7261 tttagcttct tggaaatccg caacttttag ttcacccaac accaaattct cagctgtttt
     7321 cgttaatggc tctttttcag tttcaacggt ttctaaaatt gcttcttcca cttgtttttc
     7381 tgtcgtttct ttttcttccg cttccgttgt agccggcgcc ttcacttctt ccttttggag
     7441 ctccgcaaaa tcaatttgga ttttggtcgg tttttccaaa gcctcaagcg tcgttacttc
     7501 atcaccaaac aaaacaattt gcccttcatt ggtcttcttt ttggtttttt tcggcaccgg
     7561 agccagcaca ttgctcatca gtttatagac tttattttcc aaaacattaa tttttgtttg
     7621 ccattcttcc gtcagtgagg atggtggtaa gtttgggggc aggttgtggt taatgctttc
     7681 caacttggtg attttttgtt tgatgccttg caattcctct tgaagaatct ctcgtagttc
     7741 atcttgatca acgttcttcg ctgggctggt ggctaaagat tgattcatgg cagcttgttg
     7801 cttcgttgtt acttcgttta aagcgcggat ttgctcttga atttgattta attgacgatt
     7861 aatttcctcc attccttggg aagaatcact cggtaaagac gctttttcta aatttcctaa
     7921 gcggtcactt agtttatgga gttctaattt gctgagttcc tgaacaaccc gattaaggcg
     7981 atattcgata tcgttgactt tgtcattact aaccatggct tcgtcttggt tggtgggagt
     8041 cgcttgcttt aatgaactaa tctcctcttc caaaacttta aaacgtttca tgaaatctaa
     8101 atcctgctcc ccaacattaa tcattttaat gatggcaact tcaagataaa tatggggggt
     8161 gatgctcgtt ttaattttag cctgaacatc actgatggta tcgacataat aaaagatttt
     8221 ggcttcgtta atcttttccg ctaatgctct aaaagccggt ttttcataaa tgtatttgga
     8281 gcttgtggaa gtatcgacat ttttaaataa taataaatca cgataaaact gtaaaatccc
     8341 gctcacaatc ttggaaattt ccttccctgc cgctaacaaa tcgttaatgg tcgctagggc
     8401 ttggttaatg ttgccggctt ccaaatattc cgctaattca atggttttat cataattcaa
     8461 attccccgta acggaattga catcatcaat ggtaatttca tcgtccgcaa acgatgccgc
     8521 ttgatcaag
//
LOCUS       DAOSGN010000005         6429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_10563_length_6429_cov_3.006119, whole genome shotgun sequence.
ACCESSION   DAOSGN010000005 DAOSGN010000000
VERSION     DAOSGN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10563_length_6429_cov_3.006119"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..2191)
                     /locus_tag="PK087_00215"
     CDS             complement(98..2191)
                     /locus_tag="PK087_00215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOH17722.1"
                     /translation="MQKNNLSKRFKTIYVALFIFLNTLNCYLLTTNFVFRDLSPYPRN
                     IFMVTNSFFGDFGLMVVFLSLALILNKNDYNRMRCLMIISLGLSFLFFAFSIYFGYYG
                     MMFSFYNLAAFSSAGGGDAVGFLFASLLTLLTYSKPFFWLSSLILVLMFCIGVRKHRK
                     NPEIRQYSFLKKTDRFSIGLSLLVVGILIMSSSLSAYQMEIDDTWYEDNNTPLYGTQT
                     IGFLNYYLYDAYSYLFTDKEGYSEEKKTEVLEQINAFREVEQISPIDGELVGNSPYEG
                     VFAGKNLLLIQMESMNNFVIGLKVEIDGQWVEVTPNLNKMLASSVYFNNYYTTVGIGN
                     TSDSDFTNMTGLYPVGPIYTVYEYANVLYPTLPRMFKAEGYRAISSHANTGIFYERST
                     LFPELFGFDKHYAEEDFTITEDQLIHTWLNDADFLKNTIDIMASDAVNQAVFAYALTI
                     SSHIPYRQPEESKARDSWFVGKPNLFPSNFHLIANETLNAQFIGYLEHCSYADYAIGQ
                     AMAYLHEKGLADSTIVALYGDHGCGFNIYEMFYENGALFQNEINEIITDSDLPTQILL
                     ERRMLSNIPFFIYNPNYEAESALLPAQTINLVRGTNSTVRTLASLFGLSTEYYFGVDA
                     LSSQKTFTYNPRNHDIFVDGMTISGTSQEYIINDDAYQDFYTEQKRQEILRVFQSHKD
                     LNDKILKYEIFPPRE"
     gene            complement(2194..2778)
                     /locus_tag="PK087_00220"
     CDS             complement(2194..2778)
                     /locus_tag="PK087_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17723.1"
                     /translation="MNKIKTFFDRYVVILVLYFLLALVLILFSVLFLDDIKAPSNMLI
                     YNIFGTLALLGYWQSYQLTSKKLRWSLAIGKTRRQIYNRYLKRLALSLGLSSLLVVYY
                     IFVFKWYVNRSFDYSNIIFLPIVTLFLSLLGFLEGLLRLNSKVALLVLILLFGGVIAI
                     VLLLPIPYYGNLVVLAGAGLLVFVNRFVMLRIKV"
     gene            complement(2783..3580)
                     /locus_tag="PK087_00225"
     CDS             complement(2783..3580)
                     /locus_tag="PK087_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17724.1"
                     /translation="MDTVAINNLIVGKSAISAVFNEPLVVGVYARNYRLVDEFLDALA
                     GLNPTQGKITYNNEDVFDNANYFQKRLLFNFAKPCVSTLRTNYLKDQIATNWELTFDE
                     NKFKKLVKELSIRSEVSIDYHYCFTKAGNTLTNYATVKALDKSLLLIKNPTVHLNSFE
                     QIDPVVSGLTDKNASSFVFLGMENLKNFAGKLDYVILIGNEQTVIVNPKLEKMMAVSG
                     EENLQEVVFRGEVNITRDVYDKAVTRLWDKQKRSYQRISVFDIEVFR"
     gene            complement(3711..5111)
                     /gene="sufB"
                     /locus_tag="PK087_00230"
     CDS             complement(3711..5111)
                     /gene="sufB"
                     /locus_tag="PK087_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOH17725.1"
                     /translation="MNDDVNKIIGDYKYGFTTDNPEVFNSGKGLNEDVIRAISQYKNE
                     PQWMLEQRLKAYRHFMKMPLPSFGPDLSFLDFQDFTYYIKASEEVKTTWEEVPEEIKI
                     TFEKLGIPEAERKYLAGVSTQFESEVVYHNTLDELKQKGVLFCDTDTALKLYPDLFKQ
                     YFGKLVPYTDNKFAALNTAVWSGGSFIYVPKGVVLDKPLQSYFRINSAQMGQFERTLI
                     IVDEGAKLNYVEGCTAPIYSKESLHAAVVEIYVKDSGYCRYSTIQNWSTNIVNLVTKR
                     ALCERQAHMDWIDGNIGSMLNMKYPACILKGEGAKGTTISIGFAGENQIQDAGAKMIH
                     LAPNTTSTIVSKSICRGGGKVNYRGTVRCGEQAIGARSHVECDTIILDNLSTSDTIPL
                     NIVQNQKARIEHEATVSKVSEEQLYYLMSRGLSESQALEMIVMGFIEPFAKELPMEYA
                     VELNQLIKLEMKGSIG"
     gene            complement(5104..5532)
                     /locus_tag="PK087_00235"
     CDS             complement(5104..5532)
                     /locus_tag="PK087_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOH17726.1"
                     /translation="MNDLENLYRQVIMDHYKNPRNKGLKGFPHVHIKNPSCGDDITVE
                     AEIINKQVKQVRHQGSGCSICCSSASVLSELMLNKSVDEARALVSKYYAMIKGEALDH
                     PEALEEAIVYQGVAKFPARIKCATIAWKAFEQILNGETDE"
     gene            complement(5525..>6429)
                     /locus_tag="PK087_00240"
     CDS             complement(5525..>6429)
                     /locus_tag="PK087_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOH17727.1"
                     /translation="DKVIVSELEHHSSLLPWMKLCERKQAQLVYVPLNNDGQITVENF
                     LSVFDNQVKVVALTYVSNVMGYITPLPEIIRFAHAKQAVVVVDAAQAVPHLAVDVKAL
                     DADFLAFSAHKMLGPTGIGVLYGKAKLLKKLAPLAYGGDMNESVSHHMVEVKPIPYRF
                     EAGTPAIAEALGLAKACEVITDIAYDRIREHDRALHAYALSQLNKISGLTVYNPKADI
                     GIITFNINGVHPHDAATFFDQKQIALRAGHHCAQLVSKWLHCEGTLRASFYLYNTKKD
                     VDLFVETVKQTVAFFENILGKQHE"
BASE COUNT         2103 a         1337 c         1163 g         1826 t
ORIGIN      
        1 aagccatctt cattcctcgt ttttactatt ataacaaact atcccccaat agacaacaaa
       61 aaccaaagac attgaggctt tggtttaagg gagctttcta ttcccgaggc ggaaaaattt
      121 catattttag gattttatcg tttaagtcct tatggctttg aaaaaccctg agaatctcct
      181 gccgtttttg ctcggtataa aagtcttggt aagcatcatc attgataatg tattcctgcg
      241 aagtcccgga gatggtcatt ccgtccacaa aaatatcatg gttgcgggga ttataagtaa
      301 aggtcttttg cgaagacaag gcatcaactc cgaagtaata ctccgtcgat aagccaaaaa
      361 gactggctaa ggtccgaacc gttgaattag ttccccgaac caaattaatc gtttgtgccg
      421 gcaacaaagc actctccgct tcataatttg gattataaat gaaaaaggga atattggata
      481 acatccgtcg ttccaagaga atctgcgttg gtaaatcgga atcggtaatt atttcattaa
      541 tctcattttg gaaaagagct ccgttctcat aaaacatttc ataaatatta aagccacagc
      601 catgatcgcc ataaagcgca acaatggtgc tgtcggctaa acctttttca tgtaaatatg
      661 ccatcgcttg cccaatggca taatcggcat aactgcaatg ttccagataa ccgataaact
      721 gggcattgag ggtttcattc gctataaggt ggaaattact aggaaacaag ttgggtttcc
      781 caacaaacca actatccctt gctttagatt cttccggctg acgataagga atgtgggaag
      841 aaatggttaa agcataagca aaaaccgctt ggtttacagc atcactggcc attatatcaa
      901 tggtgttttt taaaaaatca gcatcgttta accatgtatg aatcaattga tcttccgtaa
      961 tcgtaaagtc ttcttccgca taatgcttat caaaaccgaa caattccgga aacagcgttg
     1021 agcgttcata gaatattccg gtgttggcat gggaagaaat agcacggtaa ccttccgctt
     1081 taaacatgcg agggagggtc ggatataaaa cattggcata ttcataaacg gtataaatag
     1141 gtcccactgg ataaagtccc gtcatatttg taaaatccga atccgaggtg ttaccaatgc
     1201 ccactgtcgt atagtaatta ttgaaataaa ccgacgaagc taacatttta tttaaattgg
     1261 gggtgacttc gacccattga ccgtcaatct ccacctttaa accgataaca aaattattca
     1321 tcgattccat ttgaattaac aataagtttt tgccggcaaa aacaccttca taggggctat
     1381 taccgaccaa ctctccgtca atcgggctta tttgttcgac ttcccggaaa gcattaattt
     1441 gttcgagcac ttccgttttc ttttcctcac tataaccttc tttgtcggta aacaaatagg
     1501 aataggcatc atataaataa taatttaaaa acccgatggt ttgggtgcca tataaggggg
     1561 tgttgttatc ttcataccaa gtatcgtcaa tttccatttg gtaagcactt aaactggaag
     1621 acataatcaa aatcccaaca accaacagtg ataaaccgat ggaaaaacgg tccgtctttt
     1681 ttagaaaaga atattgacga atttcggggt ttttacgatg tttgcgaaca ccgatgcaaa
     1741 acattaaaac gaggattaac gatgacagcc aaaagaaggg tttggaataa gtcaaaaggg
     1801 ttaaaagtga agcaaataga aaaccgaccg catcgccgcc accggcggaa gaaaaagcgg
     1861 ctaaattgta aaaagaaaac atcattccgt aataaccaaa gtagatggaa aaagcaaaaa
     1921 aaagaaaact aaggcccagc gaaataatca ttaaacagcg catgcgatta taatcatttt
     1981 tattaagaat gagggctaaa ctgagaaaaa caaccattaa accaaaatca ccaaaaaaac
     2041 tattagttac cataaagata ttacgcggat aggggcttaa atcacggaat acaaaattgg
     2101 tggttaacaa atagcaattg agggtgttta aaaaaataaa caaggccaca tagatggttt
     2161 taaaacgttt ggaaagattg tttttctgca taattacacc ttaattcgaa gcattacaaa
     2221 acggttaaca aaaactaaca aaccggctcc cgccagcaca actaaattcc cgtaataagg
     2281 aatcggtaat aacaacacga tggcaataac tcctccaaaa agaaggatga gaaccaaaag
     2341 cgccactttc gagtttaagc gaagcaagcc ttccaagaaa cctaataagg ataaaaaaag
     2401 cgtcacaatt ggcaaaaaaa tgatgttgga ataatcaaaa gagcgattga cataccattt
     2461 gaaaacaaaa atataataaa caaccaacaa acttgataag cccaaggaca gggctaagcg
     2521 tttaagatag cgattataaa tttggcggcg ggttttgccg atggctaagg accatcgtaa
     2581 tttcttgctc gtcaactgat aactctgcca atagcccaaa agggctagtg ttccaaaaat
     2641 gttataaatc aacatattgg acggagcttt aatatcatct aaaaaaagaa cggaaaataa
     2701 aatcaaaact aaagctaaca aaaagtaaag gacaagtata acgacataac gatcaaaaaa
     2761 agtttttatt ttattcatat atttacctaa acacttcaat atcaaagacg gaaatccgtt
     2821 gatatgaacg cttctgttta tcccaaagtc tcgttacggc tttatcgtaa acatccctgg
     2881 taatatttac ttccccccga aaaacgactt cctgtaaatt ttcttcgccg cttaccgcca
     2941 tcattttctc cagtttcggg ttgacaataa cggtttgttc attcccaatt aatatcacat
     3001 aatcgagttt accagcaaaa ttttttaaat tttccattcc taaaaacacg aacgaagagg
     3061 cgtttttatc cgttaaacca ctaacaaccg ggtcgatttg ttcgaaacta tttagatgaa
     3121 cagtggggtt tttaatgagg agaagcgatt tatctaaagc tttgaccgtc gcatagttag
     3181 tcagcgtgtt tccggctttg gtaaagcaat aatgataatc aatgctgact tccgaacgaa
     3241 tgcttaattc tttcacaagc ttcttaaact tgttttcatc aaaagtaagt tcccaattgg
     3301 tggcaatttg gtcttttaaa taattcgttc ttaaagtgga aacacagggt tttgcgaaat
     3361 taaaaaggag gcgtttttgg aaatagttgg cgttatcaaa aacatcttcg ttgttgtaag
     3421 taattttccc ttgggtcggg tttaagccgg ccaaagcatc caaaaactca tcaactaaac
     3481 gataatttct ggcataaaca ccaacaacaa gcggttcatt aaaaacggcg ctgatggcgg
     3541 acttaccaac tattaaattg ttaattgcca ctgtatccat atttccacct tgacttatta
     3601 tactaaaaat caacaaagaa gaaaagatta aatcacaaaa aagaccttat tcctcgtctt
     3661 taataaaaaa gccccttaac taataatcct tgagctaaga ggttttaatg ttaaccaatg
     3721 gaacctttca tttctaattt aatcaattgg tttaattcaa cagcatattc catgggcaat
     3781 tccttcgcaa aaggctcaat aaagcccatc acaatcattt ctaaagcttg gctttcgctt
     3841 aaaccgcgac tcatcaaata atataattgt tcttcactga ctttggaaac cgttgcttcg
     3901 tgttcaatcc gcgctttttg gttttgaaca atatttaaag ggatggtatc gcttgtggaa
     3961 agattatcta aaatgatggt gtcgcattcc acatgacttc ttgctcctat ggcttgttca
     4021 ccgcaacgga ccgtcccccg ataattgact tttcctccac cccgacaaat cgatttggaa
     4081 acaatcgtgc tggtggtgtt gggtgctaaa tgtatcattt tggcaccggc atcttgaatt
     4141 tggttttccc cggcaaaacc aatcgaaatc gttgttccct tggcaccctc accttttaaa
     4201 atgcaagccg gatatttcat attaagcatc gaaccgatat tgccatcaat ccaatccata
     4261 tgagcttggc gttcacacaa agctcttttg gtaactaaat tgacaatatt ggttgaccag
     4321 ttttgaatcg ttgaataccg gcaatagcca ctgtctttta catatatctc cacaaccgcc
     4381 gcatgcaatg attctttgga ataaatcggc gccgtgcagc cttccacata atttagtttc
     4441 gctccttcat caacaattat caaagtgcgt tcaaactgcc ccatttgggc ggaattaatg
     4501 cgaaaataag attgcaaagg tttatccaaa accacccctt tgggaacata aataaatgag
     4561 cccccgctcc aaacagccgt gtttaaagcg gcaaatttgt tgtccgtgta gggaaccagt
     4621 tttccaaaat attgtttgaa taaatcggga tacagtttta aagccgtatc ggtatcacag
     4681 aacaaaaccc ctttttgttt gagttcatcg agggtgttat gataaacaac ttccgattca
     4741 aactgcgtcg aaaccccggc taagtacttt ctttcggctt cgggaatacc cagtttctca
     4801 aaagtgattt tgatttcttc cggaacttct tcccaagtgg ttttaacttc ctcgctcgcc
     4861 ttaatgtaat aagtaaaatc ctgaaaatct aaaaacgata aatcgggtcc aaacgacggt
     4921 aaaggcatct tcataaaatg gcgataagct tttaaacgtt gctccaacat ccactgaggt
     4981 tcgtttttat attgtgaaat agcccgaata acatcttcgt ttaaaccttt tccagaatta
     5041 aagacctccg gattgtcggt agtaaagcca tatttataat caccaattat tttattaaca
     5101 tcatcattca tcagtctcac cattcaatat ttgttcaaaa gctttccagg caatagttgc
     5161 acatttgatg cgagccggaa acttggccac accttggtaa acaatcgctt cttcaagggc
     5221 ttctggatga tcaagagctt cacctttaat catcgcataa tatttagaaa ctaaagctct
     5281 agcttcatca accgatttat taagcatcag ttcggataaa acactggcgc tcgaacaaca
     5341 aatcgagcat cctgagcctt gatgacggac ttgtttaact tgcttattta taatctccgc
     5401 ttcgacagta atatcatctc cacaagaagg atttttgata tgcacatggg gaaagccttt
     5461 taagcccttg ttgcgtggat ttttatagtg atccataatc acttgtcgat ataaattctc
     5521 taaatcattc atgttgttta cctaaaatat tttcaaaaaa agcgaccgtt tgcttgacag
     5581 tctccacaaa caaatcaaca tcttttttgg tattgtataa ataaaaggaa gctcgaagcg
     5641 tcccttcaca atgtagccac ttggaaacta actgggcaca gtgatggccg gcccgtaaag
     5701 caatctgttt ttgatcaaaa aaggttgccg catcatgggg atgaacgcca ttaatattaa
     5761 aagtgattat cccaatatca gccttgggat tataaacggt aaggccggaa atcttgttta
     5821 attggcttaa ggcgtaggca tgtaaagccc tgtcatgttc acgaatcctg tcataagcga
     5881 tatcagtaat tacttcacaa gctttcgcca agccaagcgc ctccgcaatg gccggtgttc
     5941 cggcttcaaa gcgataagga atcggcttaa cttccaccat gtggtgggaa acagattcgt
     6001 tcatatctcc gccataggct aagggcgcca atttctttaa caacttagct ttaccatata
     6061 aaaccccgat gccggttggt cctaacattt tatgagcgga aaaggctaaa aaatccgcat
     6121 ccaacgcttt aacatcaacc gccaaatgcg gaacggcttg agccgcatca acgaccacga
     6181 ccgcttgttt ggcatgggcg aaacgaatga tttcgggaag aggcgtaata taacccatga
     6241 cattggaaac ataggtaagg gccacgactt tcacttgatt gtcaaaaacc gacaaaaagt
     6301 tttccacagt aatttgtcca tcattattta acggaacata caccaattgg gcctgcttgc
     6361 gttcacataa tttcatccac ggtagtaatg aactatgatg ttccaattcg gaaacaatta
     6421 ctttatcgc
//
LOCUS       DAOSGN010000006         6417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_10593_length_6417_cov_3.439642, whole genome shotgun sequence.
ACCESSION   DAOSGN010000006 DAOSGN010000000
VERSION     DAOSGN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10593_length_6417_cov_3.439642"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK087_00245"
     CDS             <1..370
                     /locus_tag="PK087_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536044.1"
                     /note="Required for the synthesis of the thiazole moiety;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOH17728.1"
                     /translation="IINGESVGQVASQTLENMVVVNEVTNIPILRPLVTYDKEEIITL
                     AKKIATFEISIRPYEDCCTVFIPKHPVIRPKIDAVKEEEARCHLDQLVNDAVEQTTII
                     ELSTFKKYSVFQSHSPKFEI"
     gene            381..956
                     /locus_tag="PK087_00250"
     CDS             381..956
                     /locus_tag="PK087_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17729.1"
                     /translation="MNEENIQPTPEEQLMDEHAQTKEKIFAIEVKMQELDAIIERFED
                     EFYRKGENYEPSEEETNKVKALIEEYRDLREQKKQLQKTIKTSIWDHFPLWMGIYALF
                     QIVFSFYLIMTQISMYFAQWFLKVVNGSTDFVFYVALFMIPFLNLVLPLLIFLLLKNK
                     VHKRMFLYIYLIHGIETLIAVGMLLYVVLKR"
     gene            953..1684
                     /locus_tag="PK087_00255"
     CDS             953..1684
                     /locus_tag="PK087_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH17730.1"
                     /translation="MKRIDSLNNPFVLKLAKLHEKKYRDQERQFLIEGEHLIKEAHQA
                     RCLQAVLITDEQMAMAGIDNYLVNPLIINKLSQTKSPTNIIGVCAFFPQAKLKGTRFL
                     LLDNVQDPGNAGTLVRSALGFVVDLVIFNSQSVDFYNDKFIRATQGALFQVPLVRMDL
                     EQAIGELKKAGTRIIGTKMQGGSNLYDFSCPKKWALLLGSEGQGIAESLWPLVDETVF
                     VPIHPRLESLNVAVAGSIILSYFNQ"
     gene            1753..1911
                     /locus_tag="PK087_00260"
     CDS             1753..1911
                     /locus_tag="PK087_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOH17731.1"
                     /translation="MDRYVCDVCGYVYDPAVGDETQGIAPGTAFEDLPDDWLCPDCGV
                     GKENFSQE"
     gene            1914..3095
                     /locus_tag="PK087_00265"
     CDS             1914..3095
                     /locus_tag="PK087_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOH17732.1"
                     /translation="MKAYQIKPQVYWVGAIDWSLRTFHGYVTDRGSSYNAYLIIDEKI
                     TLIDNVKSYLVEEMMARIASVIDPSQIEIIISNHGEPDHSGSIKELIKLAPKAKIYAQ
                     APNGVKILQATYGDLPIVPVKNGEQLKIGKRTLSFYHAPMVHWPDNMVVYDQEDKILF
                     SNDIFGQHMASSERFDGDLPLEMILFEAKKYYANIVLPYTKQAARIFAAAKELDIKMI
                     ATSHGIIWRDHLPEIFQLYEYLTQSQKQTKAVVVYDTMWQSTEKIAVEIAETFSELAI
                     PVRLYNINDTDASNIMTEIIDAKYLAVGSSTINNNMLPTIAGFLCYLKGLAPVGLSSF
                     AFGSYGWGGQSIAQIEEQLDTLGFKRLMPGIRINYVPKPEDLKQIHDDIAQALHQAKT
                     V"
     gene            3221..4477
                     /locus_tag="PK087_00270"
     CDS             3221..4477
                     /locus_tag="PK087_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage repair protein"
                     /protein_id="HOH17733.1"
                     /translation="MGMRHILCIDLKSFYASVECSLRGLDPFAVNLVVADKERGGGSI
                     VLAVSPHLKKYGVNSRCRIYELPTNVEIIFAKPRMQKYIDFSGMIYDIYLQYVSAEDI
                     HVYSIDEAFLDLSSYLKYYQQPIEEIAKRIMEDIFQKTKITAACGIGTNMFLAKVALD
                     CLAKQNPNRLAYLDEQIFMDQIGDLRPLSKIWGIGLRTARHLERMGIYCLRDLANYSV
                     EKLQKTFGLVGLELYNHAHGIDKTTVQEARNYLPAHFSFGHGQALYEDYTKDEAKIVL
                     LEMVDELVTELIMRKQTCQTISLGVGYSQTVGGGFNRQLSFDKHTNSRKTIWEAFIGL
                     YEKYTKNVPIRRLNLRLGKLANEVYLQADIFDGMDKTQKEHDLYQALGEIKKRFGKNA
                     VFLALSKTPKGTQIKRNQLIGGHNAE"
     gene            4467..4781
                     /locus_tag="PK087_00275"
     CDS             4467..4781
                     /locus_tag="PK087_00275"
                     /inference="COORDINATES: protein motif:HMM:NF020438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HOH17734.1"
                     /translation="MPNDERQGKWQAFDGLDGFRKRIKKIEQRRVSSSKPVLMADELA
                     LLDEKLKKAIDSQNEVEIVFFQKGSFWRLRGVVSKVDFVFKEIIVANKKISVSTITQI
                     ND"
     gene            4846..5448
                     /locus_tag="PK087_00280"
     CDS             4846..5448
                     /locus_tag="PK087_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH17735.1"
                     /translation="MKNQFINILKMRRSHYALDKKTTIADGEIVKLVEEAVYLTPSAF
                     NSQSARAVVLFHKDHDRLWDIVTEELRKIVPEDRFAATDVKMQAFKKAYGTVLFFEDQ
                     SVVQNLQERFPLYRDVFPLWSNQSAGMLNLVVWTALAENGLGASLQHYNPIIDAEVTK
                     AFNLPSNWKLIAQMPFGVPNGPLNEKTNIPIAERVKVFGK"
     gene            5589..6086
                     /gene="msrA"
                     /locus_tag="PK087_00285"
     CDS             5589..6086
                     /gene="msrA"
                     /locus_tag="PK087_00285"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOH17736.1"
                     /translation="MKRIVLAGGCFWGVEAYYKQIKGVVDTNVGYTNGNKAKPSYEDL
                     KNHLATHAEAVEIYYDEKILSLEQILDHLFRIIDPTSLNRQGGDFGIQYRTGVYYQSE
                     EDKHRIEQFFHHQNQQLQGKLAVELAKEKGFYLAEDYHQDYLDHNPNGYCHVDFSLIH
                     EHEKK"
     gene            6107..>6417
                     /locus_tag="PK087_00290"
     CDS             6107..>6417
                     /locus_tag="PK087_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17737.1"
                     /translation="MFKKTKLLALFTSLVILALLLFVGCQEPKEEIKDDPDPVIVDPT
                     LSFEQTAFELEIGEEKTIIPVIGQTTAALTVEYEVAKPAVCEVNNHVIKALAAGETTI
                     TA"
BASE COUNT         1997 a         1116 c         1378 g         1926 t
ORIGIN      
        1 tattattaat ggcgaaagtg ttggtcaagt ggcttcccaa acccttgaaa atatggtggt
       61 ggttaatgaa gtcacgaaca ttccgattct aagaccgctt gtgacttatg ataaggaaga
      121 aatcattact ttagcgaaaa aaattgcgac cttcgaaatt tccattcgcc cctatgaaga
      181 ttgctgcacc gtttttattc ccaaacatcc ggttatccgc ccgaaaatag acgctgtaaa
      241 agaagaagaa gctcgctgtc atttagatca attagttaat gatgctgttg agcaaaccac
      301 gattattgaa ctaagcactt ttaaaaaata ttctgttttc caaagtcatt cccccaagtt
      361 tgagatttaa ggagtttcat atgaacgaag aaaatattca accaacacct gaagaacaat
      421 taatggatga gcatgcccaa accaaagaaa aaatctttgc gatcgaagtt aagatgcaag
      481 aacttgatgc catcattgaa cgctttgaag atgaattcta ccgcaaaggc gaaaactatg
      541 aaccaagcga agaagaaaca aacaaagtaa aagcccttat agaagaatat cgcgacttgc
      601 gtgagcaaaa aaaacaactt caaaaaacaa taaaaacatc tatttgggat cattttcctt
      661 tgtggatggg aatttatgct cttttccaaa ttgttttttc gttttattta attatgacgc
      721 aaatcagcat gtattttgcg cagtggtttt taaaggttgt taatggcagt accgattttg
      781 tcttttatgt agccttgttt atgattccct ttttaaattt agttttgcct ttgcttattt
      841 tcttactatt aaagaacaaa gtgcataaga gaatgttttt atatatttat ttaatccatg
      901 gtatagaaac cttgattgct gtggggatgc tgctttatgt ggtattaaaa agatgaaaag
      961 gattgattcc cttaataatc ccttcgtttt aaaactggcc aaattgcatg aaaaaaagta
     1021 ccgcgaccaa gaacgacaat ttttgattga aggagaacac ttaattaaag aagcccatca
     1081 agcccgttgt ttacaagctg tcttaattac ggatgaacaa atggcgatgg cgggcattga
     1141 caattattta gtcaatccct taattattaa caaattgtcg caaactaaat caccaaccaa
     1201 tatcattggt gtttgtgctt tttttccgca agcaaaactc aaaggaacaa ggtttttgct
     1261 attggacaat gtacaagatc ccggtaatgc cggaacattg gttcggagtg ccctcgggtt
     1321 tgtggttgat ttggtgattt ttaattcgca gtccgttgat ttctataatg ataaatttat
     1381 tcgggctact caaggagcgc ttttccaagt gccgcttgtg agaatggatt tggaacaggc
     1441 cattggggaa ttaaaaaaag cgggaacaag gattattggc actaaaatgc aaggaggaag
     1501 taatttgtat gactttagct gtcctaaaaa gtgggcttta cttttaggaa gtgaaggcca
     1561 aggcattgcc gagtctttgt ggccgctggt ggacgaaact gtttttgtgc cgattcatcc
     1621 gcgtttagaa tcattaaatg tggccgtagc cggcagtatt attttgtcct atttcaatca
     1681 ataatcaatt ttgttgtggt aataaaaact atcgtgtata attaaagaag ttttaaatga
     1741 aggaggaaaa ttatggatag atatgtttgt gatgtctgcg gttatgttta tgatcccgcc
     1801 gttggtgatg aaacccaagg aatcgccccc ggcaccgctt ttgaagattt accggatgac
     1861 tggctatgcc cggattgcgg tgttggcaaa gaaaactttt cgcaagagta aaaatgaaag
     1921 cttatcaaat taaaccacaa gtttattggg tcggagcgat tgattggtcg ttgcgaactt
     1981 ttcatggtta tgttacggat cgcggttcaa gctataatgc ttatttaatt attgatgaga
     2041 aaatcacttt aattgacaat gtaaagtcct atttagtcga ggaaatgatg gcccggattg
     2101 cttccgtaat tgatccctca caaattgaga ttattatctc taatcatggg gaaccggacc
     2161 acagcggcag tattaaagag cttattaagc tggctcctaa agccaaaata tatgctcaag
     2221 cccccaatgg tgttaaaatc ttacaagcta cttatggtga tttacccatt gttcccgtta
     2281 aaaacgggga acaattaaag attgggaaac gcactttaag cttttaccat gctcctatgg
     2341 tgcactggcc ggacaatatg gttgtttatg accaagagga taaaattctt ttcagtaacg
     2401 atatttttgg gcagcatatg gcgtcttcgg agcgctttga tggcgattta ccgctggaaa
     2461 tgattctttt tgaagccaaa aaatattatg ccaatattgt cttgccatat acaaaacaag
     2521 cggcccggat tttcgcagcg gctaaggaac ttgatataaa gatgattgcg acaagccatg
     2581 ggattatttg gcgagaccat cttccggaaa tctttcaatt atatgaatat ttgacgcaat
     2641 cgcaaaaaca aactaaagcc gttgttgttt atgacacgat gtggcaaagt accgaaaaaa
     2701 tagccgtgga gattgcggaa acattttccg aattagctat tcccgttcgg ctttataata
     2761 ttaatgatac cgatgcttct aatattatga ccgaaattat cgatgcgaaa tatttggcag
     2821 ttggttcttc aaccatcaac aataatatgt taccgacgat agccggattc ttgtgttatc
     2881 taaaaggttt agcgccggtt ggtttgtcta gtttcgcttt tggtagttac ggttggggtg
     2941 gccaaagcat tgcgcaaatt gaagaacaat tagatacttt aggttttaag cgcttaatgc
     3001 ccggaattcg cattaactat gttcccaagc cggaagattt aaaacaaatt catgatgata
     3061 ttgcgcaagc gcttcatcaa gcaaagacag tttaaaaagc tgtctttttt cattaggaaa
     3121 ttaccccttg ttatcatttt ttcatgaaaa actttcagta aaaacgccta gactatcaaa
     3181 aaaagccatg ttacaatacg cttgaaggta ggaagatctt atgggaatga ggcatatcct
     3241 ttgtattgat ttgaaaagtt tttatgcatc ggtggaatgt tcactgcgag ggcttgatcc
     3301 ctttgccgtt aatttggtgg tcgccgataa agagcgcggt ggcggctcga ttgtcttggc
     3361 cgtttcgccg catttaaaaa agtatggtgt caattcccgt tgtcggattt atgaattgcc
     3421 gacgaatgtg gaaatcattt ttgcaaaacc gcgcatgcaa aaatacatcg atttttccgg
     3481 aatgatttat gatatttatt tacaatatgt ttcagcggaa gacattcatg tttatagcat
     3541 cgatgaggca tttttagatt tatcatccta tttgaaatat tatcaacagc cgattgaaga
     3601 gattgcgaaa aggattatgg aagacatttt ccaaaaaacc aaaattaccg ctgcttgcgg
     3661 gatagggacc aatatgtttc ttgcgaaagt tgcccttgat tgtttagcca aacaaaaccc
     3721 gaaccgtctt gcttatttag atgaacaaat atttatggac caaatagggg atttacgacc
     3781 gcttagtaag atttggggga ttggtttacg cacagcgagg catttagaac ggatggggat
     3841 ttattgttta cgggacctag ccaattattc agtggaaaag ctccaaaaaa catttggcct
     3901 tgtcggtttg gaattatata atcatgctca tgggattgat aaaaccaccg ttcaggaagc
     3961 tcgcaactat cttccggcgc atttttcttt cggccatggc caagctttgt acgaagatta
     4021 tacgaaggat gaggcgaaaa tcgtcttatt ggaaatggtt gatgaattgg taacggaatt
     4081 aattatgcgt aagcaaacct gtcaaactat tagtttgggg gttggatatt cgcaaacggt
     4141 cgggggtgga tttaaccgtc aactttcctt tgataagcat accaacagcc gcaaaaccat
     4201 ttgggaggct tttattggtt tatatgaaaa atatacaaag aatgtcccca ttcggcggct
     4261 caatcttcgt ttaggaaaac ttgccaatga ggtctatctt caagctgata tctttgacgg
     4321 tatggataaa acacaaaaag aacatgattt gtatcaagct cttggggaaa taaaaaagcg
     4381 ctttggtaaa aacgcggtct ttttagcgct tagtaaaaca ccgaagggca cccaaattaa
     4441 gcgtaatcag ctaataggag gtcacaatgc cgaatgatga acgacaaggt aaatggcaag
     4501 cctttgatgg tttggacggt tttcggaaaa ggattaaaaa gatcgaacaa aggcgggttt
     4561 cttcaagcaa accggttttg atggcggatg aattagcttt gttggatgag aaactaaaga
     4621 aagccatcga tagtcaaaat gaagtggaaa ttgttttttt tcaaaaagga agtttttgga
     4681 ggcttcgcgg tgttgttagc aaagttgatt ttgtttttaa agaaataatt gtagcgaaca
     4741 aaaaaatcag cgtttccaca atcacgcaaa ttaacgatta agcaaattaa tagaaaacaa
     4801 tcattattct tgttataata aacttgctat gtaaaggagt ttattatgaa aaatcagttt
     4861 attaatatct taaagatgcg tcgtagtcac tacgctttag ataaaaaaac cacaattgcc
     4921 gatggggaaa ttgtgaaact tgtggaagag gcggtttatt tgacaccgtc cgcttttaat
     4981 tcacaatctg cacgtgcggt ggtgcttttc cataaagacc acgatcgctt atgggatatc
     5041 gttaccgagg aattgcgtaa aattgttccg gaggaccgct ttgctgccac cgatgtgaaa
     5101 atgcaggctt ttaaaaaagc ttatggaacg gtgctttttt ttgaagatca aagtgttgtt
     5161 caaaacttgc aagaacgctt tccgctgtat cgtgatgtgt ttccgctttg gtccaaccaa
     5221 agtgcaggca tgctgaattt agtggtttgg acggctttgg cagaaaatgg cttaggggct
     5281 tcgctccaac actataatcc tattattgat gcggaagtta ctaaggcatt taaccttcca
     5341 agcaactgga agttgattgc acaaatgccc tttggtgttc ccaacggtcc attgaatgaa
     5401 aaaaccaata taccgattgc tgaacgcgtc aaagtgtttg ggaaataaaa cggtgagcca
     5461 cgaatgatgg tggctctttt tcctttcttt ttggggtatt tcttgttata atatggtcaa
     5521 gaaaaaacgg tctttgcact gcttttttgc tttacttaaa gtaagctaaa taaaccggga
     5581 gaaacaagat gaaaagaatt gtgcttgcgg gtggatgctt ttggggtgtt gaagcctatt
     5641 ataaacagat aaaaggagtt gtagacacca atgtcggtta taccaacggc aacaaagcta
     5701 agcccagcta tgaggattta aaaaaccatt tggcgaccca tgcggaagcg gtggaaatat
     5761 attatgatga aaaaatcctt tctttagaac aaattttaga ccacttattt cgtattattg
     5821 accccaccag ccttaatcga caaggcggtg attttggtat tcaatatcga accggagttt
     5881 attatcaaag cgaagaagat aaacatcgaa ttgaacagtt tttccatcac caaaaccagc
     5941 aattacaagg gaaattggca gtcgaattag ccaaggaaaa gggtttttat ttagcggaag
     6001 attatcatca agattattta gaccataatc ccaacggcta ctgccatgtg gattttagtt
     6061 tgattcatga gcatgaaaaa aagtaagcaa attaaggagc aacgatatgt ttaaaaaaac
     6121 gaagctatta gccctcttca ctagcctggt tatcttggcc ttgttgctgt ttgtaggctg
     6181 ccaagaacca aaggaagaaa ttaaggatga ccccgatccc gtcatcgttg atcccacttt
     6241 gagttttgaa caaaccgctt ttgaactgga aattggtgaa gaaaaaacga ttatccctgt
     6301 gattggccaa acaacggcgg ctttaacagt cgaatatgaa gtagctaagc ccgctgtttg
     6361 tgaagttaac aatcatgtca ttaaagccct cgcagccggg gaaacgacga ttacggc
//
LOCUS       DAOSGN010000007         5904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_11890_length_5904_cov_3.767995, whole genome shotgun sequence.
ACCESSION   DAOSGN010000007 DAOSGN010000000
VERSION     DAOSGN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11890_length_5904_cov_3.767995"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PK087_00295"
     CDS             <1..638
                     /locus_tag="PK087_00295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOH17738.1"
                     /translation="TINKLFKAGANVIVNSPLTDTHTSGHASETELKIMLSLTKPKHF
                     MPIHGEYAMLKRHAQLAIATGVDPQNIHILDNGEVLTFAPNKVFTHYAVKAGAIYVDS
                     NNEDVDNSIIRERKLMSDDGMLGIVFSTLNYKLMRLPNVVSRGFIYVRDSEALVTDIE
                     NKAKELYENYYSETKKFNPNQFYNLLLPTLADFIYEKTERKPMIVPIIMNV"
     gene            complement(719..1579)
                     /locus_tag="PK087_00300"
     CDS             complement(719..1579)
                     /locus_tag="PK087_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17739.1"
                     /translation="MEIYGKVIATNPKDRIIKLQCPKRICFFYMTRRQFKDYGTYFYQ
                     HPYVFIDVEKEKKRYGTQLCYEVNYFRKIVKGTRTKKTTYYNIDNIRKDLKKLINNTK
                     MKLFLDLEFSLPSYYQTMVHIPEILQYGMIVEDENKQVVFQEGSLVKPLRRFAINNRT
                     LKFLSKTNEQFEDAKSYVEFYQLLEQCLETYNPKVIAWGKNDILTMEQSFRLNHLKPL
                     AIRSRYINLMQVIKNYYNTNTDLGLFSTYQDMSGIVVEQQQHDAMEDAIMMRKIYHLF
                     TKIVEKEVKN"
     gene            complement(1591..3330)
                     /gene="uvrC"
                     /locus_tag="PK087_00305"
     CDS             complement(1591..3330)
                     /gene="uvrC"
                     /locus_tag="PK087_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006838249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOH17740.1"
                     /translation="MDLKDKLKQLPKKPGCYQMFNQDREIIYVGKAKNLFNRVRSYFV
                     GSHDAKTTRLIANIADFEYIVTSTETEAFILEINLIKKHKPKYNISLTDDRQYPYILI
                     TDEKDPKITYTREMHRRGKFYGPYPNATAAKEVVDLLNRIYPLRKCSQLPKKECLYYH
                     LGQCLAPCIKEVKPEQYQDIISKIQSLLKGNVKEQQKQLEKQMNEASENLDFEKAIEY
                     RQLIADFNTITEKQKMELDVADTDVFAYVVRDSYLSIQVFHIRGHKTIARNGFLFEVM
                     GNPEEMFVDFIAQFYLERNFPLPKEVLIPSVDYSSLAELIKNRIVVPKKGQKRQYVML
                     VKENAMERLDVLIAQEQKQYEKTLGAVVELGKLLAIPLPKRIEAFDNSHNQGMQSVSA
                     MVVYEDGLPKKQQYRKYKIKTVVGANDYQSMYEVISRRYRPREGSTVFPNLIIVDGGK
                     PQVSSALKALADLKLSIPVLGLGKDDKHRTAYLFFNNQEIYLDKKSPLFFFLENLQDE
                     VHRYAITFHHLLASKTTLASQLDDIKGIGKVRKRQIIDVIKRANQDSIREELQQLKLS
                     EEQMEAVIAILNR"
     gene            complement(3353..3691)
                     /gene="rbfA"
                     /locus_tag="PK087_00310"
     CDS             complement(3353..3691)
                     /gene="rbfA"
                     /locus_tag="PK087_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOH17741.1"
                     /translation="MAYKLERLEKNIERELGMLFLADVKNELLKQVTITKVSLNNDLS
                     IATVYYSVYGNDEQKQAITGALTEAKGYLRSMLSKVLAVRKTPDLRFKFDESWEYGNK
                     IDNILKDLKL"
     gene            complement(3695..5899)
                     /gene="infB"
                     /locus_tag="PK087_00315"
     CDS             complement(3695..5899)
                     /gene="infB"
                     /locus_tag="PK087_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOH17742.1"
                     /translation="MVIKKLSKKYGVDIKPIRVKKEAPKPAPPKPEPLVETPQAQPVK
                     PAPKPEAKPVSKPAPKPEVKVKPEDKPKPLKPEDKPKPLKPEDKPKPLKPEPASVEVE
                     ELELSRVYDDKYNDYETEAKVYTRIKNVKKGRSRSRGNRQTNITGNDKAKANVLVFTP
                     GMTVTQLADGLNVPMNEIVRKLVMLGYMMSATAVVEKEVVEIIAEEYSFTVKDKVLED
                     ITKFEEIVIEDEEKLLKPRPPIVTIMGHVDHGKTTLLDTIRHSKVASGEAGGITQHIG
                     AYQVKKSGKLITFIDTPGHAAFTEMRARGANVTDIVVLVVAADDGVMPQTKEAVEHAQ
                     AAKVPIIVAINKMDKPSANPDRIKQELSNYDLVPEEWGGKTIYVPLSALTGKGVEDLL
                     EMILLTAELENYRANPNRLGVGLVVEAKLDRGRGPVATLLVKNGTIKIGDPIVVGNTF
                     GKIRAMQDETKTQLKQAGPSKPVEITGLSEVPLAGDHFMVFDDEKVARLVSEERTIRT
                     FNQEMGVGRPVSLANLFGDVNNPTKDLNIILKADVQGSVEALKSSLEKLNVEGINIKV
                     IRGSVGAVTETDVSLAIASSSIIIAFNVRPSSQIMEYAKEKNIEIRLYNIIYKVLEDI
                     EAAMKGMLEPVFAEKTLGQAEVRSIFKASKIGTIAGCYVTNGLILRSADVRLIRDAIV
                     IYESKISSLKRFKDDVKEVKAGYECGITIENFNDIKEGDIIEAYTMEEVKED"
BASE COUNT         1661 a         1259 c         1047 g         1937 t
ORIGIN      
        1 gtacgattaa taaacttttt aaagccggag ctaatgtcat cgtcaacagc cccttaaccg
       61 acacccatac ttccggacac gccagcgaaa cagaactaaa aatcatgttg tcgctaacca
      121 aacccaagca ttttatgcct attcatgggg aatacgccat gttgaaacgc catgcgcaat
      181 tggcgattgc caccggggtt gatcctcaaa acatccatat tctggacaac ggtgaagtat
      241 taaccttcgc tcccaacaaa gtctttacac attatgccgt taaagccggg gctatttatg
      301 ttgactccaa caacgaagat gttgacaaca gcatcattcg ggaacgcaaa ctcatgtccg
      361 atgacgggat gctcgggatt gttttttcga ctctcaatta taaattaatg cgcttaccaa
      421 atgttgttag ccgaggtttt atttatgtaa gagactcgga agcactagtg accgatattg
      481 aaaacaaggc taaagaactg tatgagaatt attacagtga aactaaaaaa ttcaacccta
      541 atcagtttta taacttatta ttaccaactt tagccgattt tatttatgag aaaaccgaac
      601 gtaaaccgat gattgttccg attattatga atgtttaaac caaaaagacg ataacaaact
      661 taaaaatgtt accgtctttt tatttgcttt attattaaat taaggagctc atttctcctt
      721 aattcttgac ttctttttca actattttcg taaacaaatg ataaatcttt cgcatcatta
      781 tggcatcttc catcgcatcg tgctgttgtt gttctacaac aatcccgctc atgtcttggt
      841 aagttgaaaa cagtcctaag tccgtattcg tgttgtaata atttttaata acttgcatta
      901 aattaatata acgagaacga atcgctaagg gttttagatg attgagccga aacgattgct
      961 ccattgttaa gatatcattt ttaccccaag caatcacttt aggattgtaa gtttccaaac
     1021 attgttctaa cagttgatag aattcaacat aagatttagc atcttcaaat tgttcatttg
     1081 tttttgataa aaactttaaa gtgcggttgt taatggcaaa cctccgtaag ggtttaacta
     1141 atgacccttc ctgaaaaaca acttgcttgt tttcgtcctc cacaatcata ccatactgta
     1201 agatttccgg aatatgcacc atcgtttgat aataagaggg tagggaaaat tccaagtcta
     1261 aaaacaactt cattttcgtg ttgttgatta atttctttaa atctttccga atattatcaa
     1321 tattgtaata agtcgttttt ttagttcgcg ttcctttaac aatttttcga aagtaattta
     1381 cttcataaca aagttgcgtt ccgtaacgtt tcttttcttt ctcaacatca ataaagacat
     1441 agggatgttg ataaaaataa gtcccataat ctttaaattg gcgccttgtc atgtaaaaaa
     1501 aacaaattcg cttcggacat tgtagtttaa taattcggtc tttaggattt gtcgcaatca
     1561 cttttccata tatttccatc ttgttcttcc ttatctattc aatattgcaa taacggcttc
     1621 catttgttct tcgcttaatt taagttgttg tagttcttca cgaatgctgt cttgattagc
     1681 acgcttaatg acatctatga tttgtctttt acgtacttta ccgattccct tgatatcatc
     1741 taattgggaa gctaaagtag ttttgctggc taataaatga tggaaagtaa tcgcataacg
     1801 atgaacttca tcctgcaggt tttcaagaaa gaaaaacagc ggactttttt tatcaagata
     1861 aatttcttga ttgttaaaga ataaataagc ggtgcgatgc ttatcatcct tccccagacc
     1921 taaaaccgga attgacagtt ttaaatccgc aagggctttt aaagcactac taacctgggg
     1981 tttgccacca tcaacaatta ttaaattcgg gaaaaccgtt gacccttccc ttgggcgata
     2041 gcgccgagaa ataacttcat acatgctttg ataatcattg gccccaacaa cggttttaat
     2101 tttatatttg cggtactgtt gctttttagg aagcccgtct tcataaacaa ccattgcact
     2161 aacgctttgc atcccttgat tgtgagaatt atcaaaagct tcaatccgct tgggaagggg
     2221 aatagctaaa agcttcccca gttccacaac cgctcccaag gttttctcat attgcttttg
     2281 ttcttgtgca attaagacat cgagtctttc catagcattt tctttaacca acatcacata
     2341 ttgccttttt tgaccctttt tggggacaac aatccgattt ttgataagtt cggctaggga
     2401 agaataatca acactaggaa ttaagacttc tttaggaagg gggaaattgc gttctaaata
     2461 aaactgagcg ataaagtcta caaacatttc ttcgggattc cccataactt caaacaaaaa
     2521 accgttgcgg gcaattgttt tatgaccacg aatatgaaag acctgaatac ttaaatagct
     2581 atcacgcact acataagcaa aaacatcggt atcagcaaca tcgagctcca ttttttgttt
     2641 ttccgtaatg gtattgaaat cggcaattaa ttgccgatac tcaatcgctt tttcaaaatc
     2701 aagattttcg gaagcttcat tcatttgttt ttccaattgc ttttgttgtt ctttcacatt
     2761 accttttaaa agggattgga ttttactaat aatgtcttga tattgttccg gtttaacttc
     2821 cttgatgcag ggagctaaac attgcccaag atgatagtaa aggcattctt ttttcggaag
     2881 ttgcgaacat ttgcgtaaag gataaatccg attcaacaaa tcaacaactt ctttggcggc
     2941 agtggcatta gggtagggac cataaaactt gccacggcga tgcatttctc gggtataggt
     3001 tattttggga tctttttcat ccgtaattaa gatatacgga tattgacggt catccgttaa
     3061 agatatattg tatttgggtt tgtgtttttt aatcaaattg atttctaaga tgaaggcttc
     3121 ggtttccgtc gaagtcacaa tatactcaaa atcagcaatg ttggcaatca atcttgtggt
     3181 tttcgcatca tggctgccaa caaaatagga cctaacacga ttaaacaaat tcttcgcttt
     3241 tcctacataa ataatttctc ggtcctgatt aaacatctga taacaaccgg gttttttagg
     3301 aagctgcttt agtttatctt ttaggtccat ttttcacctc actaaaaagg ctttataatt
     3361 ttaaatcttt taaaatatta tcaatcttgt ttccgtactc ccaagactca tcaaatttaa
     3421 aacgcaaatc cggggttttg cgaacggcca acactttgct taacatggag cgcaaatatc
     3481 ctttggcttc cgtaagcgca ccagtgattg cttgtttttg ttcatcatta ccataaacgg
     3541 aataataaac cgtcgctatc gataaatcat tatttaacga aaccttggta atcgtgactt
     3601 gctttagcag ctcgtttttc acatcggcca aaaaaagcat tcctagttcg cgctcaatgt
     3661 ttttttctaa acgttctaat ttataagcca tgccttagtc ttccttaact tcttccatgg
     3721 tataagcttc aataatgtcg ccttctttaa tgtcattgaa attctcaatg gtaatcccgc
     3781 attcataacc ggctttaact tctttcacat catctttaaa gcgttttaag gacgaaatct
     3841 tggattcata aataacaatg gcatcccgga ttaaacgaac atcagccgaa cgaaggatta
     3901 aaccattcgt aacataacaa cccgcaatcg tcccgatttt ggatgcttta aaaatcgacc
     3961 taacttccgc ttggccaagc gttttttctg caaacaccgg ttcaagcatg cccttcatcg
     4021 ccgcttcaat atcttccaag actttataaa tgatattgta taagcgaatt tcaatattct
     4081 tttccttcgc atattccatt atttgtgaac ttggacgaac attaaaagca ataataatcg
     4141 aggaagaggc gattgctaaa ctgacatcag tttcggtaac ggccccgaca ctgccacgga
     4201 tgactttaat gttaatgcct tcaacattca gtttttccaa tgagcttttt aaagcttcca
     4261 cggagccttg aacatcggct ttcaaaataa tatttaaatc tttggtcgga ttgttgacat
     4321 cgccaaagag gttcgctaag gataccggtc gtccaacccc catttcttgg ttgaaagtgc
     4381 ggatggtgcg ttcttccgaa actaatcgtg cgactttttc atcatcaaaa accataaagt
     4441 gatcaccggc taaagggact tcggataaac cggtaatttc caccggtttc gatggtcccg
     4501 cttgttttaa ctgggttttg gtttcatctt gcatagcccg gattttacca aaggtattac
     4561 cgacaacaat cggatcacca attttaatcg tcccgttttt cacaagcaaa gtggcaaccg
     4621 gaccacgacc gcggtcaagt ttggcttcca caaccaaacc gacgcctaag cggttcgggt
     4681 tagcacgata attttccaat tccgccgtca aaagaatcat ttccaataaa tcttcaacgc
     4741 cttttccggt tagtgcggaa agcggaacat aaatggtttt gcctccccat tcttccggaa
     4801 caaggtcata attgcttaat tcttgtttaa tccggtcagg attagccgac ggcttatcca
     4861 ttttattaat agccacaata attggtactt tggccgcttg ggcatgttca accgcttctt
     4921 ttgtttgtgg cataacaccg tcatcggcgg cgaccaccaa cacgacaata tcggtaacat
     4981 tggcgccacg agcccgcatt tcggtaaagg cggcatgacc cggcgtatca atgaaagtaa
     5041 tcaatttgcc gcttttttta acttggtaag cgccgatgtg ttgcgtaatt cctccggctt
     5101 cgcccgaggc tacttttgaa tggcgaatgg tatccaaaag agttgttttc ccgtgatcaa
     5161 catgacccat aatggtcaca atcggaggac gtggttttaa tagtttttct tcatcttcaa
     5221 tgacaatctc ttcaaattta gtgatatctt ctaacacttt gtctttgacg gtaaaagaat
     5281 attcctccgc aataatctca accacttctt tttcaaccac cgccgttgca ctcatcatat
     5341 aaccaagcat aaccagtttg cgaacaattt cgttcatggg aacatttaaa ccatccgcta
     5401 attgggtaac cgtcatgccg ggcgtaaaaa caagcacatt ggctttggct ttatcattgc
     5461 cggtgatatt ggtttgccga tttccccgac tccttgaacg accctttttt acattcttaa
     5521 tccgcgtata aactttagct tccgtttcgt agtcattata tttatcatca taaacgcggc
     5581 tcaattccaa ttcttccacc tcaacactcg ctggttccgg tttaagcggt ttcggtttgt
     5641 cttccggttt aagcggtttc ggtttatctt ccggtttaag cggtttcggt ttatcttccg
     5701 gtttcacctt cacttccggt tttggcgctg gtttcgaaac cggtttggct tccggtttcg
     5761 gggccggctt aacgggctgg gcttgaggcg tctccacaag cggttccggt ttcggtggtg
     5821 ccggtttggg tgcttccttc ttgaccctaa tgggcttgat gtccacacca tattttttgc
     5881 ttagtttttt aataactatc ggtt
//
LOCUS       DAOSGN010000008         5472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_13198_length_5472_cov_3.272660, whole genome shotgun sequence.
ACCESSION   DAOSGN010000008 DAOSGN010000000
VERSION     DAOSGN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13198_length_5472_cov_3.272660"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PK087_00320"
     CDS             complement(<1..563)
                     /locus_tag="PK087_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17743.1"
                     /translation="MKKIIINLSITIALVGLMVAALFTLPVHPVIKVNFLATDYLNKT
                     FAYQISTLVLSFVIILIMGLLTRFQSVKLLALNKIDGEINPEPWIGFLKKEKKENWKV
                     NGFIITIIITLVTAIVIYFQIYKKGTIQPFTIGNFLIVVLLALINSFVEEITFRHTFA
                     SIVEYHNLNRYISQALSALIFGIGHYFG"
     gene            complement(560..1078)
                     /locus_tag="PK087_00325"
     CDS             complement(560..1078)
                     /locus_tag="PK087_00325"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOH17744.1"
                     /translation="MSTKHALLGLLNIKKMSGYDLAKFAKESIGYFWNESYSNVHRTL
                     NLLAQDKLIKKIPEPGARNKIIFEITAEGKQELSKWLSNHQYTIIYRDELLLKLFVSN
                     EQDYPALLVDLKTLLQELENQQEIYLVMQKSIPNIKKKLSFNLVLDYGIIQNEITIKW
                     LKKTIKKIEENL"
     gene            1256..1570
                     /locus_tag="PK087_00330"
     CDS             1256..1570
                     /locus_tag="PK087_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOH17745.1"
                     /translation="MDVQLKRGLLEVGVLAVIRNEPSYGYRIIKDMEPYVSLSESTLY
                     TILKRLEDTKMLRVKTVEHNSRLRKYYHITKEGMDKIEEFKEYWADIKALAKLIIGEE
                     AI"
     gene            1567..2160
                     /locus_tag="PK087_00335"
     CDS             1567..2160
                     /locus_tag="PK087_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HOH17746.1"
                     /translation="MTKDKFLEELRLKLKGLPKDDLEDRIAFYSEMIDDRMEEGLSEG
                     EALKEIGTSDEVAAKVIEKTPLTKIIKEKVKPKRALKALEIILLVLGFPLWFPVLIVF
                     LVFILVCMISLWSVVITLWAVEGGLIVGAFNGVISALGLLFEGEYLNALAHLGLGALS
                     AGLAIFLFFGCWAANKYTFKLTQKITFKIKKLIVGKE"
     gene            2166..3137
                     /locus_tag="PK087_00340"
     CDS             2166..3137
                     /locus_tag="PK087_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOH17747.1"
                     /translation="MKKTTKILIIVATVLVVVGLSIFLIVLAQNDFDITKLDDHRTIV
                     REEILTEDFNNIAIDVEIDDIIFLKTEEASAKVICKESEKLKFQINVVNDTLEIKAED
                     NNKWYRRFTFGFRFKTEQISIYLPKNNYHVLTVNNDVGNLKVPAGFTFTKIDVEVSTG
                     NIEILSKVNNLNIETSTGDVKLSNLSADNINVETSTGKHTLENINVTNQIKLDTSTGT
                     VTLKSVNLATLRVAISTGRVNLTDVVATNSFNINSSTGRVSLEKCDAAEIYIKTSTGS
                     VVGTLLTSKIFHTKSDTGDINVPKTITGGICDIETGTGDIDITILNP"
     gene            complement(3617..4306)
                     /locus_tag="PK087_00345"
     CDS             complement(3617..4306)
                     /locus_tag="PK087_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOH17748.1"
                     /translation="MSSICGQQKLYASDGKKYNSDVINEDGIKLLITIIPSKYRKEMQ
                     NWIKAMLDPIDEQSKKKAYDFFKTNLIKKEEIGKTISLQKIHAYLFEGLYPFAGQIRT
                     KTISKDGFVFAVGDFLSSILKDIDRMPDTTFEEIVDKYLEMNIAHPFMEGNGRSTRIW
                     LDLLLKERISKCIDWSKIEKKEYLSAMKESPYNPSSICELLKKALTEDVNNREMFLKR
                     IDYSYYYEEDE"
     gene            complement(4736..5317)
                     /locus_tag="PK087_00350"
     CDS             complement(4736..5317)
                     /locus_tag="PK087_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOH17749.1"
                     /translation="MSQISITSNLEYIGQNVFCNSKITEFILPSTVKTIESGAFMSCT
                     SLTSISLPQNLEYIGSFAFTSSGLKEISIPANVTTANGVFSGCQSLTKAVFQSGFKKI
                     EASTFAGCSNLTSLTIPDTVTTIGNAAFAYCPALMQIFIPNSVTTIGSTIFNGCQTTL
                     QIRCAAASKPSGWNSQWNYNDGKAMPVVWGATN"
BASE COUNT         1822 a          881 c          900 g         1869 t
ORIGIN      
        1 ccaaagtagt gaccaatccc aaagattaaa gctgatagtg cttgtgagat ataacggttc
       61 aaattatggt attcgacgat acttgcaaag gtatggcgaa aagttatctc ttcaacaaaa
      121 ctatttatta aagcgagtaa gacaactatt aaaaagttgc caattgtaaa aggttgaata
      181 gtaccttttt tataaatttg gaagtagatg actatcgcag taaccaaggt aattataatt
      241 gttattataa atccatttac tttccaattt tctttctttt ctttttttaa gaatccaatc
      301 catggttctg gattgatttc tccatcaatt ttatttaagg ctaataactt aactgattga
      361 aacctagtta gaagtcccat aatcaagatg atgacaaaac ttaaaactag tgtcgaaatt
      421 tggtaagcaa aggttttgtt taaataatct gttgctagaa agttaacttt aattactgga
      481 tgaaccggta aagtaaagag tgccgcaacc attaatccga cgagggcaat ggtaattgag
      541 agattgataa taattttttt cacaagtttt cctctatttt tttaattgtc ttttttaacc
      601 atttaatggt gatttcattt tggatgattc cataatctaa aacgagatta aatgaaagct
      661 tctttttaat attggggatt gatttctgca taacaagata tatttcttgt tggttttcca
      721 gttcttgtaa aagagttttt aaatccacta ataaggcggg gtaatcttgt tcattactaa
      781 caaataactt taacaacaac tcatcacggt atataatcgt atattggtga ttcgataacc
      841 attttgataa ttcttgtttt ccttccgcag taatttcaaa aatgatttta tttcttgctc
      901 ctggttcagg tatcttttta attaacttat cttgagccaa taaattaagt gtgcgatgaa
      961 cattgctata tgattcgttc caaaaatagc cgattgattc tttagcaaac ttagcaagat
     1021 cataacccga catcttttta atgtttaata atcctaataa agcatgttta gttgacattt
     1081 aattccctct ttctttccca tcattaattc tttcacctca ttatacatat atctattaga
     1141 taggtcaaat aatacgctct ctaaattttt aaaaaaaatt taaattattt tcaaaatact
     1201 tgttgacaaa aatcatactt cgtgatacga tgtattatgt aaaaggaggt atgccatgga
     1261 tgttcagtta aagcgggggc tcttggaagt aggcgtacta gcggtcattc gaaatgagcc
     1321 ctcatatgga taccgtatta ttaaagatat ggagccctat gttagtcttt ccgaatcaac
     1381 tttgtatacc attttaaaga ggttggaaga taccaagatg ttaagggtga aaaccgttga
     1441 acataattca agactgcgga agtattatca tattactaaa gaaggaatgg ataaaattga
     1501 agaatttaaa gagtattggg ctgatatcaa agccttggct aagttaatta ttggggagga
     1561 agccatatga caaaagacaa atttttggaa gaacttaggt taaaactaaa aggtttacct
     1621 aaagacgatt tagaggaccg catcgctttt tatagcgaaa tgattgacga ccgaatggaa
     1681 gaaggattaa gtgaaggcga agcccttaag gaaattggca cttccgatga agtagccgca
     1741 aaagtaattg aaaaaactcc cctcactaaa atcatcaagg aaaaagtgaa accaaaaaga
     1801 gccctaaaag ctttagaaat tattttacta gtattaggat ttcccctatg gtttccggta
     1861 ttaattgtct ttttagtatt cattttagtt tgtatgattt ctttatggtc cgttgtcatt
     1921 actttatggg ccgttgaagg cggattgatt gtcggggcgt tcaatggtgt cattagcgcc
     1981 ttggggctac tttttgaagg tgagtattta aatgccttag cacatttggg tctaggagca
     2041 ttatcagccg gtcttgccat ttttctcttt tttggctgtt gggccgctaa taaatatact
     2101 ttcaagttaa ctcaaaaaat aacttttaaa attaaaaaat taattgttgg taaggaataa
     2161 gaacaatgaa aaaaacaact aaaattttaa taatcgttgc taccgtctta gtggtagttg
     2221 gactatcaat ctttttaata gtcttagccc aaaatgactt tgatatcacc aaattggatg
     2281 atcatcgcac tattgtacga gaagaaattt taaccgaaga ttttaataat atcgctattg
     2341 atgtggaaat tgatgatatt atcttcctta aaaccgaaga agccagcgct aaagtaattt
     2401 gcaaggaatc ggaaaaactt aaattccaaa ttaacgtcgt aaatgacact ttagaaatca
     2461 aagcggaaga taataataaa tggtatcgtc gtttcacttt tggatttaga tttaaaaccg
     2521 aacaaataag catctactta ccaaaaaata actatcatgt attgacagtg aacaatgatg
     2581 tcgggaatct caaggtgcca gccggtttta cctttaccaa aattgatgtg gaagtatcaa
     2641 ccggcaatat tgaaatttta tctaaggtca ataatcttaa tattgaaact tccaccggag
     2701 atgtcaagtt atcaaatcta agtgctgaca acattaatgt tgaaacatca accggaaagc
     2761 atactttgga aaatattaat gttactaatc aaatcaagtt agataccagt accggaacgg
     2821 taactttaaa aagtgttaac ttagctactt taagagttgc gatttcaacc gggagagtaa
     2881 atttaaccga tgtcgttgct accaacagtt ttaacattaa ttccagcacg ggaagagtca
     2941 gcttagaaaa atgtgatgcc gccgaaattt atatcaaaac ttcaaccgga agtgtggttg
     3001 ggacactgct taccagtaaa atattccaca ctaaatccga taccggcgat atcaatgtcc
     3061 ctaaaacaat tactggcggg atttgtgata ttgaaacagg aaccggagat attgatatca
     3121 cgattcttaa tccttaaata gtaataagtt taaattaaag cgtaataaaa aggtccaaag
     3181 ttttgggcct tttgttagtt ggtttttacg tttgatatca ttaaatggta atacggagga
     3241 aaattaatcc tttttttgca acttaaaatt ttattttgat ttccttgaat attaattctc
     3301 ttatttcatg ccatgataaa gacggatttt cttcataata tccatattta taagtgaaaa
     3361 tttcgttatg aaaactctta ttatgttgag taattgaagc agcccgattg tgggcattta
     3421 aaacatcaat gatggtgaat gttttaaaaa tactttttaa gttgtcttct ctttttgcaa
     3481 atgtcaactt aaaacagttt tatatattca agacaataaa ataataaact tgtattagtt
     3541 tgatactatg ctaccatctt ttttaaaaag atattggttt ataataggta aatattttat
     3601 cctaatttat tcgcctttac tcgtcctcct cataatagta tgaatagtct atcctcttta
     3661 ggaacatttc gcgattatta acatcttcag tcaaagcttt ctttaataat tcgcaaatag
     3721 atgaaggatt ataaggactt tctttcatgg cagacaaata ttctttcttt tcaatctttg
     3781 accaatcaat acatttacta attctttctt taagaagcaa atctaaccaa attctcgttg
     3841 atctcccatt tccttccata aagggatggg cgatattcat ttcaagatat ttatccacta
     3901 tttcctcaaa agtagtatca ggcatcctat caatatcttt taaaattgac gacaaaaaat
     3961 caccaacagc aaaaacaaat ccatcttttg aaatcgtctt agttcttatt tgaccggcaa
     4021 aagggtacaa accttcaaat aaataggcat gaatcttttg taaagagatt gtttttccaa
     4081 tttcttcttt ttttattaaa ttcgttttaa agaagtcata agctttcttt ttgctttgtt
     4141 catcaatcgg atcaagcata gctttaatcc aattttgcat ctcttttcta tacttggaag
     4201 gaattattgt gataagtagc tttattccat cttcattaat aacatctgaa ttatattttt
     4261 taccatcgct agcatataat ttctgttgtc cacaaattga tgacaactta aagcattaag
     4321 aacaattgaa tttttatttt tttaaaatcc aacttgttga aagtgattct ttcaagcttt
     4381 tcaataaacg aagaaataat ttgtaaatgc tcttcctgga aactgtaaac aacgataaca
     4441 tggtctaatt tatacaccta ggtgtataat ctaggtgtat aattctacaa ctaaaaaata
     4501 aacaaaaact gcggaaaact ccggttacaa caaatgtctc cccaaataat cttccttttt
     4561 atacatttaa aataaatgtt ttaataatct tggcaacaaa tttaagtttt tttactcata
     4621 ataacaccga catttatatt tttaggaaca atgtccttat gaggctttat ccaacaaaaa
     4681 agggccatgt ttctctctta agcgagattg aacatagacc ctctttacaa actttttaat
     4741 ttgttgctcc ccaaacgacc ggcattgctt ttccgtcatt gtaattccac tgactgttcc
     4801 aaccacttgg cttagatgcg gctgcgcatc tgatttgcaa tgttgtttga caaccgttaa
     4861 agatggtcga cccgattgtt gttacactgt tgggaataaa gatttgcata agggcagggc
     4921 agtacgcaaa cgcagcattt ccaatcgttg ttaccgtatc gggaatagta agcgatgtca
     4981 gattagaaca accggcaaat gttgatgctt caattttctt aaaacctgat tggaacaccg
     5041 ctttagttaa tgattgacaa ccactgaaaa caccattagc ggtagtaaca ttagctggaa
     5101 tgcttatttc tttaaggcca ctactcgtaa aggcgaatga tccaatgtac tctaagtttt
     5161 gcggtaatga aatacttgta agtgaagtac aactcataaa ggcaccggat tcaattgttt
     5221 tgactgtgct tggaagaata aattccgtta ttttggagtt acaaaaaaca ttttgaccaa
     5281 tatattcaag gttactggta atgcttattt gggaaatatt atttgaataa tatgggtgag
     5341 aactaccatc ggtgccaaat gctacatcag gaagccttgt cgcatccgta ataataactt
     5401 ccgttaaact aatcggaata taatactttt tattattgtc actatttggg tcggtgcata
     5461 tttaggtact ga
//
LOCUS       DAOSGN010000009         5340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_13657_length_5340_cov_2.610218, whole genome shotgun sequence.
ACCESSION   DAOSGN010000009 DAOSGN010000000
VERSION     DAOSGN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13657_length_5340_cov_2.610218"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK087_00355"
     CDS             <1..498
                     /locus_tag="PK087_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOH17750.1"
                     /translation="PIVGLMNIGTEPGKGRELERETFDILQKDENITFAGNIEGKEIF
                     STSCDILITDGFTGNMIMKTSEGVAKAVGVFLKQEIKKNLRSKLGYLFLKPVFKNFKK
                     ILSPDEVGGAHLFGVDGVVVKAHGSSDAYAFSNAIKQARNAVVGKVIEKMKKIIGEQN
                     EVISE"
     gene            491..1183
                     /gene="rnc"
                     /locus_tag="PK087_00360"
     CDS             491..1183
                     /gene="rnc"
                     /locus_tag="PK087_00360"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018883538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOH17751.1"
                     /translation="MNNVPEKVKELAHHLHLPLQRWDLYQLAFTHQSYANEHHEASNE
                     RLEYLGDAILDFLVAEFLYHRYPDLPEGQLTKIRAKYVCANANSKYAQALKLDRYLLL
                     GKGELEQGGKSKPSVLADLFEAFLGALYLDLGIEVVRSVLEKYVFNQIETMDTGFFVD
                     YKSRLQEFVQAESREGVRYVLQEASGLAHDKTFRFAVFHENLRLGVGVGKSKKEAQQN
                     AAKDALEKLALN"
     gene            1194..1814
                     /locus_tag="PK087_00365"
     CDS             1194..1814
                     /locus_tag="PK087_00365"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOH17752.1"
                     /translation="MYTLLKNNIIDFDRLLLEKYRALKLDEANTIILIKLNNLLKQGK
                     RFLVVEELVETMALSKEEVAKRIVQLVNDGFVALELSEIDAKEQFNLDETYKRLSYLL
                     EGVKQANQENHVNDKIKALVKVLEKEFKKILSPIELEMVSKWHIEYQYTDAAIEEAII
                     KTLRYKNRSINYVDRLLRLNHQEVKKEPSTTENIQELFNKVYVHSK"
     gene            1798..2421
                     /gene="nth"
                     /locus_tag="PK087_00370"
     CDS             1798..2421
                     /gene="nth"
                     /locus_tag="PK087_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003136515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOH17753.1"
                     /translation="MFTANEILEQLVKLYPVAHSDLHFQNHYQLTIAVVLSAQTTDVL
                     VNRATKTLFLAYPDFASLSKAKIPDVLAHIRFLGLANTKAKNIILLSQKVMAEKQGVL
                     PGDWDYLLSLPGIGRKSANVILSEGFGIPRIAVDTHVLRVSNRLGLVQTNNPLVVEET
                     LMKLYDPSVWHSLHLRFVFFGRYLCKAKKPSCALCPFHSICTYEKKS"
     gene            complement(2428..4020)
                     /locus_tag="PK087_00375"
     CDS             complement(2428..4020)
                     /locus_tag="PK087_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOH17754.1"
                     /translation="MSFDGVFIHNLLQEITPQLIKQRINKVNSLDQSNFILSLSNQKH
                     LLIIMQADSPHLRMAEGDFIFDQKTSPLLNMLKKHLEGAIIKKLVQKDNDRLVMMDSE
                     NENHLGAKTTTHLIMEFFGRNANLILVDAQMKVIECAKKIFTLGEHEKRIMLPKLAYT
                     FPQDNKLNPFLEKELASQNLYSGVSALLYQELIKQNSFSLIHQTALPTMIKTATKNYF
                     YCFDLPSIVGERTYFPNLSALLEHFYVHLKAQNTLNVEQKLVENFLKKEITKLTNKLE
                     KQKQEQQQAETNLIYEQYGNLLQANLYRIQKGDSTITVANFYNHDIPIEIPLDPLLEP
                     TKNLHQMFAKYKKAKRTLIHLQEQIELTKNDLQYFQCLENQARQAKKSDLTEIMQELG
                     LGKSKTSPKKKTKPLLLTYEDHYGHSIIVGKNNVQNNYLTHSLAKKHDYFFHVKDAPG
                     SHTILQAENPSFETLNLAAEIAAFHSPQKHSGQVAVDYTLVKNVKKVPKMKGSFVTYT
                     KQKTLFVTPDWEHIKTKIIKKS"
     gene            4131..4751
                     /gene="gmk"
                     /locus_tag="PK087_00380"
     CDS             4131..4751
                     /gene="gmk"
                     /locus_tag="PK087_00380"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOH17755.1"
                     /translation="MKLDLKGLLIVISGPSGVGKGTVRSALFNMSGHDLVYSISMTTR
                     KPREGEVNGREYYFVSKEEFQKYIQEDKMLEYAEFVNNYYGTPRDKVMELLEQGKEVV
                     LEIEVQGAEIVREKMPEAIFIFIAPPSMQDLYKRLLSRGTEPQEVIAERVQKAQREIK
                     LAYLYDYIVVNDTVENAADKIMAIIRAEHAKVERTIHQYKEMLEVK"
     gene            4752..4964
                     /gene="rpoZ"
                     /locus_tag="PK087_00385"
     CDS             4752..4964
                     /gene="rpoZ"
                     /locus_tag="PK087_00385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOH17756.1"
                     /translation="MNNTHDGMRYPSIDQLVSITKSKYKLVVGTAKRARKIEKGGNIL
                     ITNPRNKKSIGIALEEILQGKVIITD"
     gene            4998..>5340
                     /locus_tag="PK087_00390"
     CDS             4998..>5340
                     /locus_tag="PK087_00390"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17757.1"
                     /translation="MKKKDSSLFIVLVMVKSIKGGVKKMFAKVVVEVPSENTDELYTY
                     KIPDEFIDNLYVGSRVFVEFGFQKIMGYVLEIIDEIDINMDVKDILDVIDFEQGLTLE
                     QIDLAKYLKEET"
BASE COUNT         1659 a          841 c         1117 g         1723 t
ORIGIN      
        1 ccgattgttg gtttaatgaa cattggaacg gaaccgggaa aaggtcgtga attggaaaga
       61 gaaacctttg acatcttgca aaaagatgaa aatattactt ttgccggcaa tattgaaggc
      121 aaagaaattt tttcgacctc atgcgatatt ttaattaccg atggttttac gggtaatatg
      181 attatgaaaa caagtgaagg ggttgctaaa gcggttggtg ttttcctaaa acaggaaatt
      241 aagaagaatt tacgctccaa attaggatat ttatttttaa aaccagtttt taagaatttt
      301 aaaaaaatcc tcagtcccga tgaagttggt ggtgctcatt tattcggtgt tgatggcgtg
      361 gtggtaaaag cccatggctc cagcgatgcc tatgcctttt ccaatgctat taaacaagcc
      421 cgcaatgctg ttgttgggaa ggtcattgaa aaaatgaaaa aaatcattgg tgagcaaaat
      481 gaggtcatca gtgaataatg tccctgaaaa agtcaaagaa ttagcccatc atcttcattt
      541 gccacttcag cgttgggatt tataccaatt ggccttcacc catcaatcat atgccaatga
      601 acatcatgaa gcaagcaatg agcgtttaga atatctaggt gatgctattt tggatttttt
      661 ggtggctgag tttttatatc atcgttatcc ggatttaccg gaaggccaat taaccaaaat
      721 tcgcgccaaa tatgtttgtg cgaatgccaa cagtaaatat gcccaagctt taaaactcga
      781 tcgttattta ttactgggta agggcgagtt agaacaaggc gggaaatcca aaccctctgt
      841 tttagcggat ttatttgaag cgtttttagg ggctttgtat cttgatttag gtattgaagt
      901 tgttcgcagt gttttagaaa aatatgtttt taaccaaatt gaaactatgg ataccggttt
      961 ctttgttgac tataagagcc gtttacaaga atttgtgcag gcagaaagcc gggaaggtgt
     1021 tcgctatgtc ttgcaagaag ccagcggttt agctcatgat aaaactttcc ggtttgcggt
     1081 tttccatgaa aaccttcgtt taggtgttgg ggttggtaaa agcaaaaaag aagcccaaca
     1141 aaatgccgcc aaggatgctt tggagaaatt agcgcttaat taaggaggtc aaaatgtata
     1201 cattattaaa aaacaacatt attgattttg atcgcttgtt attggaaaaa tatcgagcat
     1261 taaaacttga tgaagccaat acgattattt taattaagct taataattta ttaaagcaag
     1321 gtaaacgctt cttggttgta gaagagcttg tggaaacgat ggccctttcc aaagaagaag
     1381 tggcgaaacg gattgttcag ttagtcaatg atggctttgt agccttggag ttgtcggaaa
     1441 tcgatgccaa agaacaattc aatttagatg agacttataa gcgtttgtct tatttattag
     1501 aaggagtcaa acaagccaat caggaaaacc atgttaacga taaaattaaa gcgcttgtga
     1561 aggttttgga aaaggagttt aagaaaatct taagtccgat tgaactagag atggtttcca
     1621 aatggcatat tgaatatcaa tataccgatg ctgccattga agaagcaatt attaaaacat
     1681 tgagatataa aaatcgcagt atcaattatg ttgatcgcct cttaaggctt aatcatcagg
     1741 aagtaaaaaa agaaccttca acaacggaga atattcaaga attgtttaat aaagtttatg
     1801 ttcacagcaa atgagatttt agaacaatta gtcaaacttt accctgttgc ccatagtgat
     1861 cttcattttc aaaatcatta ccaattaaca attgcggtgg ttttgtccgc gcaaacaacg
     1921 gatgttttag ttaatcgggc cactaaaact ttgtttttgg cttatccgga ttttgccagt
     1981 ttaagtaaag ctaaaatccc cgatgtttta gcgcatattc gctttttagg tttagccaac
     2041 accaaggcca aaaatattat cttgctttcc caaaaagtga tggcagaaaa gcaaggcgtt
     2101 ttacccggtg attgggatta tttattaagt ttgccgggaa tcggccgaaa aagtgccaat
     2161 gttattttaa gtgaaggttt tggcattccg cggattgctg ttgatactca tgttttaagg
     2221 gtttccaatc gcttgggttt agtccaaaca aacaatccct tggtggtcga agaaaccttg
     2281 atgaaacttt atgatccgtc cgtttggcat tcccttcatt tacgcttcgt cttttttggg
     2341 cgctatcttt gtaaagctaa aaagcctagt tgtgctcttt gtccatttca ttcaatttgt
     2401 acttatgaaa agaagtccta acaccagcta agacttctta attattttag ttttaatatg
     2461 ctcccaatca ggagtgacaa ataaggtttt ttgtttagtg taagtgacaa atgagccctt
     2521 cattttcgga acttttttaa cattcttcac aagtgtataa tcaacagcga cttgaccgga
     2581 atgtttttgc ggggaatgga aggcagcaat ttccgctgcc aagttgagcg tctcgaaaga
     2641 aggattttcc gcctgtaaaa ttgtgtgact tcccggtgca tctttcacat ggaagaaata
     2701 atcatgtttt ttagccaagg aatgggttaa ataattgttt tgcacattgt ttttgccaac
     2761 gataatgctg tgaccgtaat ggtcttcata agttaataaa agcggtttgg tctttttctt
     2821 aggactagtt ttacttttac cgaggcctaa ttcctgcatg atttctgtta agtcgctttt
     2881 cttggcctgg cgagcttgat tttccaaaca ttggaaatat tgtaaatcat tcttagttag
     2941 ttcaatttgc tcttgtaaat ggataagcgt gcgcttggcc ttcttgtatt tcgcaaacat
     3001 ttgatgaagg tttttagttg gttctaaaag tggatcaagc ggaatttcga tagggatgtc
     3061 atgattgtaa aaattggcga cggttatggt ggaatcgcct ttttggatac gatataaatt
     3121 ggcttgcagg agattaccgt attgttcata aatgagatta gtttctgctt gttgttgctc
     3181 ctgtttttgt ttttccagtt tattagtcaa tttggtgatt tcttttttta agaagttttc
     3241 aacaagtttt tgttcaacat tgagggtgtt ttgggctttc agatggacat aaaaatgctc
     3301 taaaagggcc gataaattgg gaaaataagt tctttcaccg acaatcgaag gtaaatcaaa
     3361 acagtaaaaa taatttttgg tagcagtttt aatcatcgtc ggtaaagcag tttgatgaat
     3421 gagggaaaaa gagttttgct tgattagttc ttgataaagg agagcggaaa ccccggaata
     3481 aagattttgg ctggccaatt ctttttcaag aaaggggttt aatttgttat cttgaggaaa
     3541 ggtataagct agttttggga gcataatccg cttttcatgt tcaccaagcg taaaaatctt
     3601 tttagcacac tcgattactt tcatttgggc atcaaccaag atgaggttgg cattcctgcc
     3661 aaaaaattcc ataattaaat gggtagttgt ttttgctccc aaatgattct cgttttcgga
     3721 atccatcatc actaagcggt cattatcttt ttgtacaagt ttttttatga tagccccttc
     3781 caaatgtttt ttaagcatat tcaaaagggg agaagttttt tggtcaaaaa taaaatcccc
     3841 ttccgccatt cgcaaatgag gagaatcggc ttgcatgata atgagcaaat gtttttgatt
     3901 gctaaggctt aggataaaat tggactgatc aaggctgttg accttgttaa tcctttgctt
     3961 aattaactgg ggggtaattt cttgcagcaa attgtgaata aaaacaccat caaaactcat
     4021 ctttaggtcc tctgagttat tttatcatat cattttgctt aattcaaaaa tttctttctt
     4081 tttttacttt ttagtagtat aataagagca aataattttt cgagggccaa atgaaattag
     4141 atctcaaagg tttattaatt gttatttccg gaccttccgg agttggtaaa gggactgttc
     4201 gcagtgcttt atttaatatg agcgggcatg atttagttta ttccatctcg atgacaacta
     4261 gaaaaccgcg tgaaggcgaa gtcaacggtc gggaatatta ttttgtttcc aaagaagaat
     4321 tccaaaaata tattcaagag gacaaaatgc tcgagtatgc ggagtttgtt aataattact
     4381 atggtacacc ccgagataaa gttatggaac tattggaaca aggcaaagaa gtcgtcttag
     4441 aaattgaagt tcaaggggcg gaaattgttc gggaaaaaat gccggaagcc attttcatct
     4501 ttattgcacc gccatcaatg caagatttat ataagcgctt gctttcccga ggaacggaac
     4561 ctcaggaagt gattgcggaa cgcgtccaaa aagcccaaag agaaatcaaa ttagcttatt
     4621 tatatgatta tattgttgtc aatgataccg ttgaaaatgc ggcggacaaa attatggcca
     4681 tcattcgggc tgaacatgcg aaagttgaaa gaacaattca tcaatacaaa gaaatgttgg
     4741 aggttaaata aatgaataat actcatgatg ggatgcgtta tccatcgatt gatcaattag
     4801 tgtccattac taaatccaaa tacaaattgg ttgtggggac ggctaaacgc gctcgcaaga
     4861 ttgaaaaagg cggaaatatt ttaatcacta atccgcgcaa caaaaaaagc atcggcattg
     4921 ccttagaaga aattcttcaa gggaaagtta ttattacgga ttgataaata gaaaacttga
     4981 cggcggtcaa gttttttttg aagaaaaagg atagttcttt atttatcgtt ttagttatgg
     5041 taaaatcaat taaaggtggg gtgaaaaaga tgtttgcgaa agtcgttgtc gaagttcctt
     5101 cggaaaacac cgatgaatta tatacataca aaatacccga tgaattcatc gataatcttt
     5161 atgtggggtc gcgcgttttt gtcgagtttg gttttcagaa aatcatgggt tatgttttgg
     5221 aaatcattga tgaaatcgat attaacatgg atgtaaaaga catccttgat gtgattgatt
     5281 ttgaacaagg cttaacatta gaacaaattg acctagcgaa ataccttaaa gaggaaacaa
//
LOCUS       DAOSGN010000010         5193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_14203_length_5193_cov_3.081938, whole genome shotgun sequence.
ACCESSION   DAOSGN010000010 DAOSGN010000000
VERSION     DAOSGN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14203_length_5193_cov_3.081938"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PK087_00395"
     CDS             <1..310
                     /locus_tag="PK087_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOH17758.1"
                     /translation="VVILKDMTTRVEGRDLLLVEDIIDTGLTLKEVTNLLKERGAASI
                     EICTLLDKPEGRTVNGVNPKYIGKSIPNKFVVGFGLDYNEIYRNLDYVGVLKESIYKH
                     "
     gene            321..2306
                     /gene="ftsH"
                     /locus_tag="PK087_00400"
     CDS             321..2306
                     /gene="ftsH"
                     /locus_tag="PK087_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOH17759.1"
                     /translation="MAQGPQLKPKKIKRADVGVIAFIILLVIGIFFFMRNATGKPDEL
                     LYNDFFTYLTNDRIETMRITPLGGENPGLYSVSGKYKTETNADGLYVIVLSEDDIEIK
                     VKPFIALNNIEMEYVGLNPADWWSIIAIVLPILLFAGLIIFFVRNSGSSNNKAFEFGK
                     TRARLSRHSKVTFADVAGVDEEKQELEEIIDFLKFPKKYFEIGARVPKGILLVGPPGT
                     GKTLLAKAVAGEAKVPFYSISGSDFVEMFVGVGASRVRDMFRVAKQTAPCIVFIDEID
                     AVGRQRGAGLGGGHDEREQTLNQLLVEMDGFSENSGVIIMAATNRADILDPALLRPGR
                     FDRQIHISNPDVRGRVDILKVHARNKKISPKINFEDLAKRTPGFSGADLENLLNEAAL
                     LAARENRKEVKIYDIDEAIDRVLMGPAKRSRKYSEREKRIVSFHEAGHAVIGVKLDNA
                     EVVQKVTIVPRGEAGGYTLMTPEEEQFLQTKNGLLDTIVGLLAGRVSEEMMTNEITTG
                     AHNDFQRATAIARAMVTEYVMSKLGPIQYERTSREVFLGRDYMKDKNFSDQVAMEIDQ
                     AVREIIDTCYQRARQVLTDNQDLLVLLADHLMEIETLTREDIYELANTGQLQWWEKKK
                     AKAEAERLAKEKEEELQEIYRKQLEAMEEAKKSINKE"
     gene            2309..2563
                     /locus_tag="PK087_00405"
     CDS             2309..2563
                     /locus_tag="PK087_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOH17760.1"
                     /translation="MRLDKYLKVARIIKRRTVAKEVGDADRITVNDRIAKPSTVLKVG
                     DIVSLRYLHKHLVFRVVSLQATVKKEDAKMMYEIIEIHDE"
     gene            2811..4043
                     /locus_tag="PK087_00410"
                     /pseudo
     CDS             2811..4043
                     /locus_tag="PK087_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193125.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4108..4806
                     /locus_tag="PK087_00415"
                     /pseudo
     CDS             4108..4806
                     /locus_tag="PK087_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            4928..>5193
                     /locus_tag="PK087_00420"
     CDS             4928..>5193
                     /locus_tag="PK087_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH17761.1"
                     /translation="MIILGQISFYLIAALSIVLGLRICVSDYDNGHIKNLMQAKLKRS
                     EITIGKAIFFVIVTVILVLTITVIALLRSKNGGSTNVLVISHGKA"
BASE COUNT         1739 a          841 c         1066 g         1547 t
ORIGIN      
        1 cgtcgtgatt ttaaaagata tgacaactcg ggtggaaggt cgggatttat tgttggtgga
       61 agacatcatt gataccggct taaccttaaa agaagtcacg aatttattaa aggaacgcgg
      121 ggccgcaagc attgaaattt gcacgctctt agataaaccg gaagggcgaa cagttaatgg
      181 cgttaatccc aaatatatag gtaaatccat ccctaataaa tttgtggttg gctttggctt
      241 ggattataat gaaatttacc gaaaccttga ttatgttggg gttttaaaag aatcgatata
      301 taaacactag gaggatttag atggctcaag gtccacaatt aaaacctaaa aaaataaaac
      361 gcgctgatgt tggggtcatt gccttcatca ttttgctggt tatcgggatt ttctttttta
      421 tgcggaatgc aacgggaaaa ccggatgaat tactatacaa tgactttttc acatatctca
      481 ctaatgaccg gattgaaacg atgcggatta ccccgcttgg tggtgaaaac cccggccttt
      541 attccgtgtc cggaaaatat aaaacagaaa cgaatgctga tggtttatac gttattgttt
      601 taagcgaaga cgacattgaa ataaaggtca aaccttttat tgcccttaat aatattgaaa
      661 tggaatatgt cggtttaaac ccggccgatt ggtggagcat tattgcaatt gttttaccga
      721 ttctcttgtt tgcgggttta attatcttct ttgtccgtaa ttcgggcagc agcaacaata
      781 aagcttttga atttggaaag actagggcta gattaagccg tcattcgaaa gtcacctttg
      841 ctgatgttgc cggtgttgat gaagaaaaac aggaattgga agaaatcatc gatttcttaa
      901 agttcccgaa aaaatatttt gaaattgggg ctcgcgttcc gaaaggcatc ctattagttg
      961 ggccgccggg aacgggaaaa actttacttg cgaaagccgt tgccggggaa gctaaggtcc
     1021 cgttttattc catctccggt agtgattttg tagagatgtt tgttggtgtc ggcgccagcc
     1081 gtgttcgcga tatgttcaga gttgcgaaac aaacagcgcc ctgcattgtt tttattgatg
     1141 aaatcgatgc tgtcggccgg caaagagggg ccggtttagg tggcggtcac gatgaacgtg
     1201 aacaaacctt aaaccaattg ttggtggaaa tggacggttt ctcggaaaac tccggtgtca
     1261 ttattatggc tgctaccaac cgcgctgaca ttcttgatcc cgctttactg cgtccgggcc
     1321 gttttgaccg ccagattcac atttccaacc ccgatgttcg ggggcgggtg gatatcttaa
     1381 aagttcatgc ccgcaacaaa aaaatctcgc cgaaaatcaa tttcgaagat ttagccaaaa
     1441 gaacgccggg tttttccggt gccgatttgg agaatttgtt gaatgaagcg gcgcttttag
     1501 ctgccagaga aaatcgtaaa gaagtcaaaa tctatgatat cgatgaagcg attgatcgcg
     1561 tcttaatggg accagctaaa cgcagtcgca aatatagcga aagagaaaaa cggattgttt
     1621 ccttccatga agccggtcat gctgttatcg gtgtgaaatt ggataatgcg gaggtcgttc
     1681 aaaaagtgac aattgttccg cggggggaag cgggcggata tacactgatg actcccgaag
     1741 aagaacaatt cttgcaaacc aaaaacggac tattagatac gattgtcgga ttattggccg
     1801 gtcgtgtttc cgaagaaatg atgactaatg aaattaccac aggtgctcat aatgatttcc
     1861 aacgggcaac cgccattgcc agagcgatgg ttaccgaata tgtcatgagt aaattaggtc
     1921 ctatccaata tgaacgcact tcacgggaag tctttttagg ccgtgattat atgaaggata
     1981 aaaatttctc cgatcaagtg gcgatggaaa ttgatcaagc ggtgcgggaa attattgata
     2041 cctgctatca acgagcccgc caggtgttaa ctgataatca agacttgtta gttttacttg
     2101 ccgaccactt aatggaaatt gaaaccttaa cacgggaaga tatttatgaa cttgctaaca
     2161 ccggccaatt acaatggtgg gaaaagaaga aagccaaagc tgaagcggaa cggcttgcaa
     2221 aggaaaagga agaagaactg caagaaattt atcgcaaaca gctagaagcg atggaagaag
     2281 cgaaaaaatc catcaacaaa gaataattat gcgccttgac aaatatttaa aagtcgcccg
     2341 gattattaaa cgacgcactg ttgcgaagga agtcggtgat gcggaccgca ttacggtcaa
     2401 tgatagaatc gctaaaccaa gcactgtttt aaaagttggc gacattgttt ctttacgtta
     2461 tcttcacaaa cacttggttt ttcgggttgt ttccttacaa gcaacggtta aaaaggaaga
     2521 tgcgaaaatg atgtatgaaa tcatcgaaat tcatgatgaa taatggaacc tggttaaggg
     2581 tcataaataa aagcattttg ttttaaaaac gaaatgcttt tttaataatt aataaacaat
     2641 ttattttcgg agaattaagg gaaatcacca agtattaaaa ataagtgaaa atgtaaaaac
     2701 cgaaaaaata aaataacttt cttgacatgc ttgattgtag tgggaaagcg tgatataatc
     2761 attttataaa agcaacgaaa tccgaaaaaa aataaaatgg aggtcttact gtggaaatta
     2821 aaagaaatat atatttgcaa aaacttattg ttagaaaaca caacggaatg attaaagtaa
     2881 tcacgggcat tcgtcgatgc ggtaaatcat atttattgtt taaacttttc aaacaatatt
     2941 tattagaaca aaatgtttgt gaagaccaca ttatagaaat taattacgat gccattgaaa
     3001 atgagtattt acaagatgct ttaaaaacct atgcttatat caaagagaaa attaaagatc
     3061 agcaaatgta ttatattttg ttagatgaaa ttcaattgct taacaaattc gaatccgtgt
     3121 taaacagttt tttaaaactt tccaatgttg atatctatgt gacaggcagt aacgcgaagt
     3181 ttttgtccag cgatgtcatt acagaatttc gtggacgtgg cgaccaagtg catgtgtatc
     3241 cattatcatt taaggaattt atgtcggtat attccggtaa tgaagatgaa ggcttgcgtg
     3301 attatatgca ctatggagga ctaccactaa ttaccaaaat gcttcaacac gaacaaaaag
     3361 cgcggtattt aaataatctt ttcgatgaaa catatttagt agatatcatt aatcgccatc
     3421 agataatgaa aacaacggaa ttggaagaat tattaaatat tctttcttct aatatcggct
     3481 ccttcacaaa tccaagaaaa ttatctaatg cctttaaaag tatcaagggt gtatccatta
     3541 ctgaaccaac gataaaaaca tttttagaat atttagaaga ttcatttttg attgaaaaag
     3601 ccattcgata cgatattaaa ggaaaaaaat atcttaatac gcctgccaag tattatttta
     3661 ccgatgtcgg gctaagaaat gctagattaa attttaggta attagaagat agccatttgc
     3721 ttgaaaatat catatataat gaattgaaaa tcagagctta cagtgttgat gtcggcgttg
     3781 tagaaatcaa taacaaagac aaaacaaaaa gctatttaga agttgatttt gtatgtaata
     3841 aagtggatca aagaatttat cttcaaacaa cattgtcaat tcctgatcaa gaaaaattgc
     3901 atcaagaaca acgcccctta gtcaatgtga atgattcttt taaaaaaatc attatcacaa
     3961 gagacaagat gaaaagccgt tatatggata acgggatatt aattatgaat gtgtttgatt
     4021 tcttattgga tgagaaagtt tagtttgcga ggaaacacgg cattatcaca atcataaaca
     4081 cgattttatt taggaaggat aaattaaatg ttagaaataa ttaaaatcaa taaaacattt
     4141 ggtaataaac aagtgttaaa taatgtttcc tttaatattc ccctgggctc aatttgtggt
     4201 ttgttaggtt taaatggggc cgggaaatcg acattaatga aaattatcac atcattggtg
     4261 ttcaaggatt ccggagaaat taaatatcac ggggaaaagt atgaaaagga taccagtttt
     4321 cttacgattg gatatatgat tgaatcccct tccttttata atgatttatc aggctaagat
     4381 aatcttttgc ttcttgccac gttgtataat aatatcggta aaaagcaaat cgaagaagtt
     4441 ttaaataaag tcggcttaca agattcaaaa gatattcccg ttaagaaata ttctcacggg
     4501 atgaaacaaa ggctttattt tgcctatgcc attttagatg atcctcagat attagtatta
     4561 gatgaaccct ttaacggcat cgatccggtt accagtaaat tatttaaaga cttaattaag
     4621 gaacgagcaa gtaaggcttg taccgttctt gtctcaagtc atgtcattag tgatgtgaaa
     4681 caaatatgtg atcaagttgt cattcttgat cgtggagtgg ttatttatga taacagcgtt
     4741 tcaaaagatg acgacttaga acaattgttt atttccaaat tatcaaacag cggtcaggca
     4801 caatgaaaac ttttattttt gaatgccgta aattccatag caataaaaag ctttctttat
     4861 tgttgccaat ctatgcggtt tattcagtat tgatgcggaa ttcttaattt atgaaaacgg
     4921 cgtttttatg atcattcttg gtcagattag tttttactta atcgctgctc tcagcattgt
     4981 tttaggactc aggatttgtg tttccgatta tgataatggt catattaaga acctgatgca
     5041 agccaaacta aaaagaagtg aaatcacgat tggaaaagcc atcttttttg taattgttac
     5101 tgttattctt gttcttacaa tcaccgtaat cgctttattg agatcaaaga acggtggatc
     5161 cacaaatgtt ttagtcatta gccacgggaa agc
//
LOCUS       DAOSGN010000011         5176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_14267_length_5176_cov_3.573130, whole genome shotgun sequence.
ACCESSION   DAOSGN010000011 DAOSGN010000000
VERSION     DAOSGN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14267_length_5176_cov_3.573130"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PK087_00425"
     CDS             complement(<1..502)
                     /locus_tag="PK087_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HOH17762.1"
                     /translation="MTKEQLLKIRKKASEKLLMSKLPHAYRVAIGMATCGVTAGAQPV
                     YDEFVAEVKKRHLNDVFVASVGCVGECALEPLVEVTDNQGVVTTYCKVKPEDVEKIIE
                     NHLVNGRIVEDLLPSKFKKGGSSDANARQGRIALRNCGIINPEDLEEYIARDGYLALA
                     KALTEMT"
     gene            complement(512..1003)
                     /locus_tag="PK087_00430"
     CDS             complement(512..1003)
                     /locus_tag="PK087_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOH17763.1"
                     /translation="MAIKFEMSPCQIDELKQRIAKHLQKPGPLMPTLHDAQQIFGCIP
                     LEVQKIIAEELNEPIAKINGVVSFYSHFTVEPKGKHIINVCLGTACYVRGSQDIIDKL
                     SELLKIKPGETTADGKFTLAANRCFGACGLAPVFTVDGKVYGTANTKDAQSAVESLLK
                     DLE"
     gene            complement(1116..3083)
                     /locus_tag="PK087_00435"
     CDS             complement(1116..3083)
                     /locus_tag="PK087_00435"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH17764.1"
                     /translation="MKYLQTIIGLLFVFLLGSLLMGCQEDVSQESTKIKDLESWVLTL
                     YQDKIIDEDQSFPKTAEGLGGVITWESSQADLLSSNGFYQAPKEDTIINLICTISIDG
                     QTKTLTIPVTVKGKDEALEPLPLLVQMENWVLALYQDKVIDQNQNFPKTAEGIGGTIK
                     WQTFDPDLLTAQGVYTAPVVDTNIELVVTIKIDGEQKILFIPVTIKGYGTPMDAISLY
                     VEKIVKQDVVNNVFLPLTHPDYDCAITWQSSRPDLLDNKGNFTKPNEDIPFELSYTIL
                     YEGESVTKILVMRAKGLSDFQKAVAVLEQLDSEYQKINNVNGDLDLMQTVDLYGAIIE
                     WESSNPSVISTTGKYQAPLYDQNVRLTLTVRVQDSHVSSTYQWTVKGGVALHKWDQIE
                     QFLKAIAKPQINTIKQFYLFGYEVGYERVPSQNQGYLPFYDEKPMTIIQEIVPMTNMN
                     IRPGRNRTATKYIVIHNTGMAAPTATAKQLSKSIQNSTREASWHFSIDDKETYQQLGI
                     NEVGWHAGEANGNNYGIGIESCVYQGVDFNQVLRRLAKLTAQLLIDFNLGFSDIKQHY
                     DFSGKNCPQVIREAHRWDEFIDLVQIEYFAMTNLSDVSFVWKSLTPTILDDEGKVIHH
                     PGRVVQVSYQVSVTYHNETRVFTFESTLNNL"
     gene            complement(3094..>5176)
                     /locus_tag="PK087_00440"
     CDS             complement(3094..>5176)
                     /locus_tag="PK087_00440"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH17765.1"
                     /translation="AIKKDLLSKVEPSARANITLPSTDEIYGATIAWKSSNEQVITND
                     GVVIQKEYDSQVQLSLTINYEGKVLSHSLPFTVIGYAYEIVADAFLEQFSPNITHSYD
                     NIKTTNAAYPNAVITWSSSNELVFTNQGVYKNPKADTPFDIEVQVGFSNQPDVYTFTK
                     QVIAKSVPPLTAIKNELLSKFETNVHMNIALPSTDEVYGSTITWESSDEQVITNDGVV
                     FQKEYNSQVQLSFTIAYEGETLTHTIPLTVVGYAYEIVADDFLKQFNKLITRNYDNIK
                     TVNYNYPNAVITWSSSNELVFTNLGVYKKPKENTPFEIEVQVGFTDQQAVHTFTKQVV
                     AQGITIYEKTAIIEEKILNSLNLGKMITESLELPIFDEEFQADLTWVSNKAAIITSTG
                     AVVTPIANQMVTLRCEVSIGNDYDSFSMEVEVAGKQYNDKWAAVEDFLSLIFHDRIKT
                     QRYTMFGATSYTAYNYGYLPFYSEAKSVILDGMVPFADRPGIVKTETRWVVIHDTANT
                     RVGADAKMHTAYIRTNPGVSWHYSVDDRETYQHIPDEEVAYHAGAREGNYYGIGIETC
                     LNQGIDYNVVMRRTAKLTAELLMEFNLSIYNVRQHNYYSGKDCPHVMRSANRWDEFLM
                     LVAIEYFALKNLSDVSFVWESLSPDILDNTGKIISQAGIETTVTYRVLVTYKNETREF
                     TKTSTLEAKSW"
BASE COUNT         1514 a         1102 c          949 g         1611 t
ORIGIN      
        1 gggtcatttc cgttaaagcc ttcgctaagg ccaagtaacc atcacgagca atatattctt
       61 ccaaatcctc cggattaata ataccgcaat ttcttaaagc aatgcgccct tgacgagcat
      121 tggcatcgct gctgccgcct tttttgaatt tgctcggtaa caaatcctca acaatccgcc
      181 cattaacaag atggttttca ataatctttt ccacatcttc cggtttaact ttacaataag
      241 ttgtaaccac cccttgatta tcggtaactt ctaccaaggg ttctaaagca cattccccta
      301 cgcagccaac ggaagctaca aaaacatcgt ttagatgacg tttcttgact tccgcaacaa
      361 attcatcata aacaggttgt gctccggccg tcactccaca agttgccatc ccgatagcaa
      421 ctcgataagc atgcggaagc ttggacatta ataatttttc cgaagctttt tttctaattt
      481 taagtaactg ttctttcgtc atggctgctc cttactccaa atccttcaaa aggctttcca
      541 cagcgctttg cgcatctttg gtgttagccg ttccgtaaac tttcccgtca acagtaaaaa
      601 ccggggctag accgcaagcc ccaaaacaac gattagcagc taaagtaaat ttcccgtctg
      661 cggttgtttc tccgggctta atcttcaata actcagataa tttgtcaata atgtcttgcg
      721 agcctcgtac ataacatgct gtccctaaac aaacattaat gatgtgtttc ccctttggtt
      781 caacggtaaa atgggagtaa aagctcacca ctccgtttat tttcgcaatc ggttcgttca
      841 attcctcagc gataattttt tgaacctcca gcgggataca cccaaaaatc tgttgggcat
      901 catgaagagt tggcattagt ggtccgggtt tttgtagatg cttcgcaatt cgttgcttca
      961 gttcatcaat ttggcacgga ctcatttcaa atttgattgc cattggtacc acctcatttc
     1021 aaaagtattt tatcactaat cacctaaaaa agatataatt ataaaacgct tacattctta
     1081 agggtaaata aaaaagaaaa cccgccggct ttcttttata aattgtttaa agttgattca
     1141 aaagtaaaaa cgcgtgtttc attgtgataa gtaacgctaa cttggtagga aacttgaaca
     1201 acacgccccg gatgatgaat tactttcccc tcatcatcga gaatcgtcgg ggttaaagat
     1261 ttccaaacaa acgaaacatc acttaaatta gtcattgcga aatactcaat ttgcactaaa
     1321 tcgataaact catcccaacg atgcgcttca cgaataactt gcgggcagtt cttaccgctg
     1381 aaatcataat gttgtttaat atcggaaaaa cctaaattaa aatcaattaa cagttgagcc
     1441 gttaatttcg ctaatcttcg taaaacttgg ttaaaatcaa cgccttgata aacacaggat
     1501 tcaatgccaa tcccgtagtt attgccgttg gcttcgccgg catgccaacc aacttcgtta
     1561 atacccagtt gttggtaggt ttccttatca tcaatggaga aatgccatga ggcctcacgc
     1621 gttgaatttt ggatggattt acttaattgt ttagctgtgg cggtcggtgc ggccatgccc
     1681 gtgttatgaa tgacgatata tttcgtcgcc gtgcggtttc gtcccgggcg aatattcata
     1741 ttcgtcatcg gcacaatttc ttgaataatc gtcatcggtt tttcatcata aaacggtaaa
     1801 taaccttggt tttgtgatgg cacccgttca taaccaactt catagccgaa caaataaaac
     1861 tgtttaatcg tattaatctg cggtttcgca atcgccttta aaaattgttc aatttggtcc
     1921 catttatgca aagcaacacc gcccttaaca gtccattgat aagttgatga aacatgactg
     1981 tcctgaacac gaaccgttaa agtcaaacga acattttggt cataaagcgg agcttggtat
     2041 ttcccggttg tggaaataac actcggattg gaagattccc attcaataat ggcgccatat
     2101 aaatcgacag tttgcatcaa atccaaatca ccattaacat tattaatttt ctgatattcg
     2161 gaatctagtt gttctaaaac cgccactgcc ttttgaaaat cacttaagcc tttagccctc
     2221 ataacaagaa ttttagttac gctttcgccc tcatacaaaa tggtataact taattcaaag
     2281 gggatatctt catttggttt tgtgaaatta cccttgttgt ccaaaagatc gggacgggat
     2341 gattgccaag tgatggcaca atcataatcc ggatgggtta agggaagaaa aacattgttc
     2401 acaacatcct gcttaactat tttttccaca tacagactaa tggcgtccat tggcgttccg
     2461 taacctttaa tggtgacggg aataaacaat attttttgtt ctccatcgat tttaatcgtc
     2521 actaccagtt caatgttcgt gtccacaacg ggggccgtat aaactccttg ggccgttaaa
     2581 aggtccggat caaaagtttg ccatttaatt gtcccgccaa tgccttcggc agtcttggga
     2641 aaattttgat tttggtcaat gaccttgtct tgatataaag ctaaaaccca attctccatt
     2701 tgtacaagca aaggtaatgg ttcaagcgct tcatccttcc ctttaacagt aaccggaatc
     2761 gttagggttt ttgtttggcc gtcaatactg attgtacaga ttaaattgat aatggtatct
     2821 tctttagggg cttgataaaa accattagaa ctaagtaaat ccgcttgact tgactcccaa
     2881 gtaatcactc cgccaaggcc ttccgcggtc ttcgggaaac tttggtcttc atcaattatt
     2941 ttatcttgat ataaagttaa tacccaactt tccaagtctt tgatttttgt tgattcttgc
     3001 gaaacatctt cttggcagcc cattaataag cttcctaaaa ggaaaacaaa caatagtccg
     3061 atgatggttt gtaaatattt cattgcctac ctcttaccat gattttgctt ccaatgtcga
     3121 agtcttcgta aattcccggg tttcgttttt ataagtaacc aaaacacggt aagtgacagt
     3181 ggtttcaatc cctgcttgtg agattatttt tccggtattg tctaaaatat ccgggcttaa
     3241 cgattcccat acaaaactaa catcgcttaa atttttaaga gcaaaatatt caattgctac
     3301 caacattaaa aattcatccc agcggtttgc tgagcgcatc acatgcggac aatctttccc
     3361 ggaataataa ttatgttggc gaacattata aatacttaag ttaaattcca tcagcagttc
     3421 cgctgttaat ttagctgtcc ttcgcatcac cacattataa tcaattcctt gattcaaaca
     3481 agtttcaatc ccgatgccgt aataattacc ttccctggct ccggcatgat aagcaacttc
     3541 ttcatcagga atatgttgat aagtttcgcg gtcatcaact gaataatgcc aagaaacacc
     3601 cggattagtc cgaatgtagg cggtgtgcat tttcgcatcc gcaccaactc ttgtgttggc
     3661 ggtatcatgg ataaccaccc agcgagtttc tgttttaacg atgcccggac ggtccgcaaa
     3721 cggaaccata ccgtctaaaa tcactgattt ggcttctgag taaaacggta aataaccata
     3781 attataggct gtataggaag ttgccccaaa catggtatag cgttgcgtct taatccgatc
     3841 atgaaaaatc aaacttaaaa aatcttcaac cgctgcccat ttgtcattgt attgtttccc
     3901 cgcaacttca acttccatag aaaaagaatc atagtcgttc ccaatggaaa cttcacaacg
     3961 caaagtgacc atttggttgg caatcggggt aaccactgcc ccggtagaag taatgatggc
     4021 ggctttattg gaaacccaag ttaaatcagc ttggaattct tcatcaaaaa tcggtagttc
     4081 taaagattcc gtaatcattt tccctaaatt caaggaattc aagatttttt cttcaatgat
     4141 ggccgtcttt tcatagatgg taataccttg agcaacgact tgttttgtga atgtatgaac
     4201 agcttgttga tccgtaaacc ctacttgaac ttcaatttca aaaggggtgt tttctttcgg
     4261 ttttttgtaa acaccgaggt ttgtgaaaac aagctcattg gaggaactcc aagtaataac
     4321 ggcattagga taattataat taacggtttt gatattatca taattgcgag taatcagttt
     4381 gttaaattgc tttaaaaaat catcggcaac gatttcataa gcataaccaa caacggtaag
     4441 gggaatggtg tgagtcaatg tttctccttc ataggcaatc gtaaaactta attgcacttg
     4501 cgaattatac tctttttgaa aaacaacacc gtcgttagta atcacttgtt catcggatga
     4561 ttcccaagta atggtggatc cgtaaacctc atcggtgctt ggcaaagcaa tgttcatgtg
     4621 gacattggtt tcaaatttcg ataaaagctc gtttttaatg gccgttaaag ggggaacact
     4681 cttcgcaatg acttgttttg tgaaagtata aacatcgggt tgattactaa accctacttg
     4741 aacttcaatg tcaaaagggg tgtctgcttt cgggttttta taaacacctt ggtttgtaaa
     4801 cacaagttca ttggaggagc tccaagtaat gacagcattg ggataagcgg cgttagtcgt
     4861 ttttatattg tcatagctat gagtaatatt aggactgaat tgttccaaaa aagcgtcggc
     4921 aacgatttca taagcatagc caatgacggt aaaaggaagg gagtgactca atacttttcc
     4981 ttcataatta atggtcaagc ttaattgcac ttgtgaatcg tattcttttt gaataacaac
     5041 gccgtcgtta gtaatcactt gttcattgga tgatttccaa gcaatagtcg ctccataaat
     5101 ctcatccgtg cttggtaaag taatattcgc tcgggcgctt ggttccacct tggataaaag
     5161 gtctttttta atggcg
//
LOCUS       DAOSGN010000012         5061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_14721_length_5061_cov_3.064123, whole genome shotgun sequence.
ACCESSION   DAOSGN010000012 DAOSGN010000000
VERSION     DAOSGN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14721_length_5061_cov_3.064123"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PK087_00445"
     CDS             complement(<1..1109)
                     /locus_tag="PK087_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17766.1"
                     /translation="MKGLFKFLFGLFIFLIITVVVPIGILYYQVSDSRDLAPVELYAD
                     DITVQSQISKMMARALESEEDEIYLAFSEEELNLLLFAVIRESVNPDYYKTTSEADTV
                     IQSMDIPTEVPLVGGKKAVLKHAYAKINGNEVSVYMTARALGIKTSLNFGLSITESEG
                     VFKLTITRLGVGKFNLLGTLGKMILKPIMKSMQITDKINEEINYNKLPIVFDESDFSF
                     SLSKADLGDLLVNLTAGDEETPNELLGQLLGVLTGTDNDLFGMGVFDADEPVFGVRLN
                     LTELKYNAVLDGAPVYRLDYQDYKTKMNLLLAANPTEHFAAMNQFLINGFDKLSPADQ
                     MTVSSLDFSSVGIAANTISSFKGLVNVPAVDLEAA"
     gene            complement(1163..1708)
                     /locus_tag="PK087_00450"
     CDS             complement(1163..1708)
                     /locus_tag="PK087_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOH17767.1"
                     /translation="MLVLIGPSASGKTELAKILINRYQMKKLVTCTTRLIRPHEIDGI
                     DYYFLTVQEFLKRQEQGEFVETTFYNNHFYGTLKKEISDEKVVVLDPSGWQALLAKMP
                     DKIISVFLKTPKEVRKQRMIMRQDAMDDIERRLANDDIVFDESKLGKVDFIINSYDIS
                     LEDLATQIYQLYHSSCQERRR"
     gene            1843..3147
                     /gene="purB"
                     /locus_tag="PK087_00455"
     CDS             1843..3147
                     /gene="purB"
                     /locus_tag="PK087_00455"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010024449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOH17768.1"
                     /translation="MIERYQTEAMKQIWTDSHKYATWWQVELAVLAAYVQKGLIPQSD
                     YEQIKLHARINEQRIKELEAILHHDVIAFTRQISETLGEEKKWVHYGLTSTDVVDTAN
                     SLLIKQANDLIEQSLLKMLNLLQKLALKYQWTPCIGRTHGMHAEITSFGLKWALYFDE
                     TKRNLNRFRQVRQEIEVGKLSGAVGNFANFDPEIERLACEQLKLTPAAIATQVLSRDR
                     HAHYLMVLALIASGIEKIALEVRHLSRTEIGEVQEFFSQTQKGSSAMPHKKNPIASEN
                     ICGLARMMRSYLQVALENNALWHERDISHSSTERLMLPDATGLLFYMLERYRPTLEKL
                     QINPQRMMENIHLTKGLIFSGQVVSLLIEKGLSREQSYDFVQQASQLALTTKKALMAV
                     LKEQGINKWLTPAELQACFTIEPYLKNTDYIYQQLHLGDSYE"
     gene            3140..4420
                     /locus_tag="PK087_00460"
     CDS             3140..4420
                     /locus_tag="PK087_00460"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOH17769.1"
                     /translation="MNKTLVVVGSQWGDEGKGKITNYLSEKADFVVRYQGGDNAGHTI
                     KFDGTTYKLHLIPSGVFNPQVTNILGNGMVVNVRNFLTEIARLQDQGFKCKNVFISDR
                     AHVLFDYHVLLDGIKEESLGANLVGTTKKGIGPAYTDKVARDGIRMIDFISDDFFTIF
                     KEKIQNKNKELIALKKEPLPFLPLYQEYQKIAEKIAPFVIDTISLLNQAYSQGKKILF
                     EGAQGALLDVDFGSYPFVTSSNASACGVCSGSGLGPTKIDAVIGVVKAYSTRVGSGYF
                     PTEFEDETAQYIRETGNEYGTTTRRPRRIGWFDGVVVQYSKMINGLTGISLMLLDVLT
                     GLETIKVCSAYKIRGKTIHTIPASIRELEKCVPIYEELPGWKEDITQVKSFDELPENA
                     KNYIRRIEQITGLDVQIFSVGPDKTQTIIRKELF"
     gene            4423..4845
                     /locus_tag="PK087_00465"
     CDS             4423..4845
                     /locus_tag="PK087_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOH17770.1"
                     /translation="MQGYNVIMVYSPDLKHLLMCKRTNEPYQGLYNLVGGKIEAHESS
                     EEAAYRELFEETGITKQDILLHHFFDFTYYYQNCYVEVHVGKLNHPVQLVEEKNHLFW
                     SDLKQNFFGPQYAGEGNIGHMVEQVRLYENELFNKKEA"
     gene            complement(4872..>5061)
                     /locus_tag="PK087_00470"
     CDS             complement(4872..>5061)
                     /locus_tag="PK087_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOH17771.1"
                     /translation="IFINGKLVYPLPDIEEIRAYCQAELETLWDEVKRFENPHNYYVD
                     LSQKLWDIKNTMIEQAKR"
BASE COUNT         1581 a         1039 c          946 g         1495 t
ORIGIN      
        1 atggccgctt ctaagtccac agccggaaca ttaaccaaac ctttaaaact actaatggta
       61 ttggcggcaa tcccaacact ggaaaaatcc aaactggaaa ccgtcatttg gtcggccgga
      121 cttaacttat caaaaccatt gattaagaat tgattcatgg ccgcaaaatg ttctgtggga
      181 ttggcagcca acaaaagatt cattttggtt ttatagtctt gataatctaa gcgataaacg
      241 ggtgccccat ccagcacagc attatatttc aattccgtta aatttaaacg aaccccgaag
      301 accggctcgt cggcatcaaa aacccccatt ccaaataaat cattgtccgt accggttaaa
      361 acaccaagca attggccaag cagttcgttg ggtgtttctt catcaccggc cgttaaatta
      421 actaataaat cgcctaaatc ggcttttgat aaggaaaagg agaaatcact ttcatcaaag
      481 acaatcggta atttgttata gttaatttct tcattaatct tgtcggtgat ttgcatactt
      541 ttcataatcg gttttaaaat cattttccct aaagttccta acaaattgaa cttaccgaca
      601 cctaaacggg taattgttaa tttaaacacg ccttcgcttt cagttatgct taagccaaag
      661 ttaaggctcg ttttaattcc caaagcccta gcggtcatat aaacggaaac ttcattgcca
      721 ttgattttcg cataagcatg ttttaaaact gctttttttc ctcccacaag gggaacttcc
      781 gtaggaatat ccatgctttg aatcacagta tccgcttccg aagtcgtttt ataataatcc
      841 ggattgacac tttcgcgaat gacggcaaac aacaacaaat tgagttcttc ttccgaaaaa
      901 gctaaataga tttcgtcttc ttcggactcc aaggcacgag ccatcatttt tgagatttgc
      961 gattgcaccg taatatcatc agcatataat tcaaccggag ctaaatcgcg ggaatcggaa
     1021 acttgataat aaagaatccc aataggaaca accacggtaa ttattaaaaa aataaataat
     1081 ccaaaaagaa atttgaaaag acctttcatg ttttttgtcc tccacaatta ttatatcata
     1141 tctattaaaa aataggtcaa aattatcttc tccgctcttg acaactactg tgatagagtt
     1201 gatagatttg ggttgctaaa tcttccaagc taatatcata actattgata ataaagtcga
     1261 cttttcccag tttgctttca tcaaaaacaa tatcatcatt agctaagcgg cgctcaatat
     1321 cgtccatcgc atcttggcgc ataatcatcc gttgttttct aacttccttg ggggttttta
     1381 aaaaaacgga gatgattttg tcgggcatct tggctaacaa ggcttgccag ccgctaggat
     1441 ctaagacaac tactttttca tcactgattt ctttttttaa agtaccataa aaatgattat
     1501 tataaaaggt tgtttctaca aactcacctt gttcttggcg ctttaaaaac tcttgaaccg
     1561 ttaaaaaata atagtcaatc ccatctattt cgtgaggacg aatcaagcga gtcgtacaag
     1621 tcaccagttt tttcatttga tagcgattaa tcaagatttt cgcaagctcg gttttaccgc
     1681 tggctgaggg gccaattaaa actaacatcg taatcctttc tctaaacaaa acttttgttt
     1741 ctcttttttg ctctcttaat tttactcgtt atcaagccaa taatcaatat ttttctttat
     1801 ttatggggtg gggtttggta taatgagttt tgggtgatga tgatgattga acgctatcaa
     1861 acagaagcaa tgaaacaaat ttggactgat tctcataaat acgccacttg gtggcaagtc
     1921 gaactggccg ttttagcggc ttatgtgcaa aaaggtctca ttccccaatc tgattacgag
     1981 caaattaagc ttcatgctcg catcaatgaa caaaggatta aggaactgga agcgatttta
     2041 catcatgatg tcattgcctt tacaaggcaa attagtgaaa cattgggtga agaaaagaaa
     2101 tgggttcatt atggactgac ttccaccgat gtagttgaca ccgcgaactc cctcttaatt
     2161 aaacaagcca atgatttaat tgaacagtcc cttttaaaaa tgcttaattt attacaaaaa
     2221 ttagccctga aatatcaatg gacgccatgc atcgggcgaa ctcacggcat gcatgcggaa
     2281 ataacttcct ttggtctaaa atgggctttg tattttgatg aaaccaagcg taatttaaac
     2341 cgttttcgtc aagtccggca agaaattgaa gtcggtaaat taagtggtgc tgtcggcaat
     2401 tttgccaatt tcgatcccga aatagaaaga ttagcctgcg aacaactgaa attaacaccc
     2461 gcagcgattg ccacgcaagt tttatcaagg gatcgacatg ctcattattt gatggttttg
     2521 gctctcattg cttccggaat cgaaaaaatc gccctggaag ttcggcactt aagcaggacc
     2581 gaaatcggtg aagtccaaga attttttagt caaacccaaa aaggctccag tgccatgccc
     2641 cataaaaaga atccgattgc cagcgaaaac atctgtggct tagcacggat gatgcgcagt
     2701 tatctccaag tggctttaga gaacaatgcc ttatggcacg aacgagatat ttctcattcc
     2761 tcaacggaac gattgatgct tcccgatgcc accggtttac tgttttacat gttagagcgt
     2821 taccgcccca ctttagaaaa actacaaatc aatccccagc gcatgatgga aaatatccat
     2881 ttaaccaagg gccttatttt ctcgggtcaa gtcgtttccc tgctgattga aaaaggcttg
     2941 agccgtgaac aaagttatga ctttgtgcaa caagcttccc aattagccct aacgactaaa
     3001 aaggccttaa tggcggtttt aaaagaacaa ggtattaaca aatggcttac gccagccgaa
     3061 ttacaagctt gctttacgat tgaaccatat ttaaaaaata ccgattatat ttatcaacaa
     3121 ttacatttag gagattctta tgaataaaac tcttgtcgtc gttggctccc aatggggcga
     3181 cgaaggaaaa ggcaaaatca ccaattattt aagcgaaaaa gccgattttg ttgttcgtta
     3241 ccaaggtggc gacaatgccg gccacaccat caaatttgac ggaacaactt acaaactcca
     3301 tttaattcct tccggtgtct ttaatcccca agtaactaat attttaggaa acggcatggt
     3361 ggttaatgtc cgcaactttt taacggaaat cgcccgtttg caagaccagg gctttaagtg
     3421 taaaaatgtg tttattagcg accgtgccca tgtcctcttt gattatcatg ttttgcttga
     3481 cggcattaaa gaagaaagcc ttggggctaa tctcgtcggc accactaaaa aaggcattgg
     3541 accggcctat actgataaag tcgctcggga cggcattcgc atgattgatt ttatttcgga
     3601 tgattttttc accatcttta aagaaaaaat ccaaaataaa aacaaagaat taatcgccct
     3661 caaaaaagaa ccccttcctt ttttaccact atatcaagaa tatcaaaaga ttgcggaaaa
     3721 aatcgcgcct tttgtgattg atacaatcag tttactaaac caagcttatt ctcaaggtaa
     3781 aaaaatcctc tttgaaggag cccaaggggc cttattggat gttgattttg gttcttatcc
     3841 ctttgtcact tcttccaatg cctccgcatg tggagtgtgc agtggttcag gcctcggtcc
     3901 aactaaaatt gatgccgtta tcggggtagt gaaagcctat tccacaagag tcggcagtgg
     3961 ttacttcccc accgaatttg aggacgaaac ggcccaatat atccgggaaa ccggcaatga
     4021 atatggtaca accactagac gaccgcgacg gattggctgg tttgatggcg tagtcgtcca
     4081 atatagcaaa atgattaacg gcctaacggg aatctcgctc atgcttcttg atgttttaac
     4141 cggccttgaa accatcaaag tttgttcagc atataaaatt agaggtaaaa caattcatac
     4201 catccccgct tccattcgcg aattggaaaa atgtgtgccc atttatgaag aattgccggg
     4261 atggaaggaa gacattactc aagtcaagag ttttgatgaa ttaccggaga atgcgaaaaa
     4321 ctatattcgc cggattgaac aaattaccgg cttagatgtc caaatttttt ccgtcggtcc
     4381 ggacaaaacg caaaccatca taaggaaaga attgttttaa taatgcaagg ttataatgtc
     4441 atcatggttt attctccgga cttaaaacat cttttgatgt gtaagcgcac aaatgagccc
     4501 tatcaaggtt tgtataacct tgtgggcgga aaaattgaag cccatgaaag tagtgaagaa
     4561 gcggcttacc gcgaattatt tgaagaaacc ggcattacca agcaggatat tttactgcat
     4621 catttctttg attttactta ctattatcaa aattgctatg tggaagtcca tgtgggcaaa
     4681 cttaatcatc ccgttcaatt agtcgaagaa aaaaatcact tgttttggag tgatttaaag
     4741 caaaacttct ttggtccgca atatgcgggt gaaggcaata ttgggcatat ggtcgagcaa
     4801 gtccgcttat acgaaaatga actttttaat aaaaaagagg cctaacatca aaaaaagatg
     4861 ctaggctttt tttatcgttt tgcttgttca atcatggtgt ttttgatatc ccataatttt
     4921 tgcgataaat ccacataata attatgcggg ttttcaaaac gcttaacttc atcccataaa
     4981 gtttccagtt ccgcttggca ataagctcta atttcttcaa tatccggaag tggataaacc
     5041 aacttgccat tgataaaaat c
//
LOCUS       DAOSGN010000013         4824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_15704_length_4824_cov_4.616062, whole genome shotgun sequence.
ACCESSION   DAOSGN010000013 DAOSGN010000000
VERSION     DAOSGN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15704_length_4824_cov_4.616062"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK087_00475"
     CDS             <1..188
                     /locus_tag="PK087_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17772.1"
                     /translation="TATPTTAPTVSHSPIPKRDKYTATMDRDLRKRLKIASVEKSIMV
                     SEFIEQAVREKLEREGF"
     gene            189..971
                     /locus_tag="PK087_00480"
     CDS             189..971
                     /locus_tag="PK087_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOH17773.1"
                     /translation="MAVISTFCKKGGVGKTTFIGYLGHYHAVHGKKVLIISVDDQNSI
                     FKIFGEEDKIFESNDNYLEFLIAGHAELGDVLIEVRENLYLIKTLNTDKLSMKLTLER
                     NQEKVIKKIVQEYDNYFDYIFIDFPPSSSRLTEVLLDISSTILIVVGLDSLGLGGFFN
                     TIQYFIDNDIDIDGIKYIVPNGYSKNRRAPKISLEKLIEQGREFTPGAIVLPALQDKS
                     IIKNLQAEGVSPFDTVKLERYDQNAKTILEKELTSLFQKIEM"
     gene            complement(1055..2179)
                     /gene="dnaN"
                     /locus_tag="PK087_00485"
     CDS             complement(1055..2179)
                     /gene="dnaN"
                     /locus_tag="PK087_00485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOH17774.1"
                     /translation="MKFVINRDLLLLNLTYVSRALSTKTPMPVLTGIKIEAYENSVFL
                     TASNNEISIQTIIKDKRQVRIEENGIVVVPGKYFIEIIRKIEAREVDFSTFEENIVKI
                     LADRSNFTLNALEKDNYPLISFADSETVVHMDVGNLKSIIRKTTFATSLSETRVILTG
                     VSFVADDRKLEVIATDSFRLAKKYLINDVEFPKINAVIPSKSLDELNRIIDENETPIE
                     IHFSPSKALFKYKNILFQTRLIDGSFPNTSNLIPVDFLTSIKFNKNEIIAAIERASLF
                     TSLDSFCIIKFTLNSDKTIEIASTTNEIGAVKEEVIPLACSNVLHFQIAFSSKYILDA
                     IKSMDSTEITIHFTGEIKPFIITGDYDVNLTQLILPVRVS"
     gene            complement(2248..3606)
                     /gene="dnaA"
                     /locus_tag="PK087_00490"
     CDS             complement(2248..3606)
                     /gene="dnaA"
                     /locus_tag="PK087_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOH17775.1"
                     /translation="MEDSLKLWQQTLLTLKNELDETDFLELFADVNEVFKTENNLIFI
                     IVPNALVKFRIEKFYLEKLNRICASITKTPCRFKFILKEDAVEQEKVSITTKDTINKR
                     PLKAEYTFDNFETGESNRFAFLSAMKVAETHDKIYNPLYIFGDVGLGKTHLMMAIGHY
                     ILDNDPNTNVVYTTAQEFTEDYFIHTSTKKGKENIEAFYQKYRRADVLLVDDIQFLEG
                     KQATQEEFFKVFETLHGLNKQIVITSDRSTTELKNMMVRLKSRFNWGLSVDIKVPDKP
                     LRMNILKRKLSFLIENPSDVPTNVLDIIADYFTNNIRDLEGALRRYITYCVSLNIPFT
                     VENVYVALERFLPERDADKSTNNGYFDLVKKEVAAYYQVQISDLESATRKQHITYARQ
                     MAIYLIKNIYNPSLKSIGERFGNRDHATVSHSVDKIEALIKSNNLVKCDFDILFKKIK
                     NN"
     gene            3892..4812
                     /locus_tag="PK087_00495"
     CDS             3892..4812
                     /locus_tag="PK087_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17776.1"
                     /translation="MNKEKNSIIYRIFNFPNKINVITMFVVVVVYGLVLLLLAKVITP
                     EADYVVELNYPPATYNSEINPYIFVRGTAINASEDENKIDLRMQYRIIAYINKMNTPY
                     PTNITYAYSAMDKGGMMNYFLEAKRSSLNVYHQVVASAEYPKGDWYEQYFVKVRYDIN
                     DGNETVHKTLSLSEKALNLKKSELNDRKFQTSPKLPGILEVKFIFTDDGATIPQYDTN
                     IQITFDEKTPYHLNFQSWIVTEGKEIFPFIGVYNYCTPEPLKPAYETSVFKFVKPKTI
                     YMKLDYTDENNVLTPFYYKVDVATLLNSGN"
BASE COUNT         1604 a          871 c          869 g         1480 t
ORIGIN      
        1 ggaccgcaac gccaacaaca gcgcccacag tttcccattc acctattccc aaacgcgaca
       61 aatacaccgc cacgatggac cgggatttac ggaaacgtct aaaaatcgct tcagttgaaa
      121 aatcaatcat ggtttccgag tttattgaac aagctgtcag ggaaaaattg gaaagagagg
      181 ggttctaaat ggccgttatt tcaacttttt gcaaaaaagg cggggttgga aaaacaactt
      241 tcatcggtta tttaggccat taccatgccg ttcatggcaa aaaagtatta attattagtg
      301 tcgatgatca aaacagcatc tttaaaatct ttggtgaaga agataaaatc ttcgaaagca
      361 atgataatta cctggaattc ttaattgccg ggcatgccga actgggcgat gtgctcattg
      421 aagtccgcga aaacctctat ttaatcaaaa cattaaacac cgataagctt tccatgaagc
      481 tcacgcttga aagaaaccaa gaaaaggtca taaaaaagat tgttcaggag tacgacaatt
      541 actttgatta catttttatt gattttcctc cctccagtag ccgtttaacg gaggttttac
      601 tggacataag ttcaaccatt ttaattgttg tgggtttgga tagtttggga ttaggtggtt
      661 ttttcaatac cattcaatat tttattgaca atgatatcga tattgacggg ataaaataca
      721 ttgttcccaa cggttacagc aaaaaccgcc gcgcccctaa aatttcttta gaaaaattaa
      781 tcgaacaagg gcgtgaattc acccccggcg ccattgtttt accggcttta caggataaat
      841 ccattattaa gaacctccaa gcggaaggtg tcagcccttt tgatacggta aaactagagc
      901 gatacgatca aaatgcgaaa actattttag aaaaagaact aactagcttg tttcagaaaa
      961 ttgaaatgta aaaaggcact atcaaaagtg ccttttttcg ttgtttataa ttatgtatgt
     1021 atgtaattac ataaatatgt tggttaaatc tagtttaaga aacacgaacc ggaagaatta
     1081 attgcgttaa attaacatca taatcaccgg ttataataaa cggtttgatt tcgccggtaa
     1141 aatggatggt gatttcggtc gaatccatgg atttaatggc atccaaaatg tattttgagc
     1201 taaaagcaat ttggaaatgc aaaacattag aacaagcaag aggaataact tcttccttta
     1261 ccgctccgat ttcattggtc gttgaggcaa tttcaatcgt tttatcagag tttaaggtaa
     1321 atttaataat acagaaggaa tctaaggaag taaacaaaga cgctcgttca attgcagcaa
     1381 taatttcgtt tttgttgaac ttaatgctgg ttaaaaagtc aaccggaatt aaattgctgg
     1441 tgttcgggaa tgatccgtcg attaagcgcg tttggaagag gatgttttta tatttaaata
     1501 aagctttgga aggcgaaaaa tgaatttcaa tcggggtttc gttctcatca atgattcgat
     1561 ttaactcatc taagctctta ctagggataa cagcattgat tttcgggaat tcaacatcgt
     1621 taattaagta ttttttggct aaacggaagc tgtctgtcgc aattacttcc agttttcgat
     1681 catcagccac gaaagaaact ccggttagga taactctggt ttccgaaagc gaggtcgcaa
     1741 aggttgtttt tcgaataatg gattttaaat taccgacatc catgtgaaca acggtttcag
     1801 aatctgcaaa agaaataagc ggatagttgt ctttttctaa agcattaagg gtaaaattgc
     1861 tacggtctgc cagtatcttt acaatgtttt cttcaaaagt cgaaaaatcg acttctctgg
     1921 cttcaatttt acgaataatt tcaataaaat acttcccggg aactacaact atgccgtttt
     1981 cttcaatccg aacttggcgt ttatctttaa taatcgtttg aatggatatt tcattattgg
     2041 atgcggttag aaaaacgctg ttttcataag cttcaatctt aattcccgtt aaaacaggca
     2101 tcggggtttt tgttgaaaga gcacggctca cataagttaa gttaagcaat aataaatcac
     2161 gattaattac aaatttcatt cttcaatcct cttttatctt tatttttagt tatttttatt
     2221 attaggggct gtggataagt ggaaaactta gttatttttt atttttttaa acaaaatatc
     2281 gaaatcacac ttaactaaat tgttggattt aatcagggct tcaatcttat caacggaatg
     2341 ggagactgtt gcatgatcac gattaccaaa tcgctcacca atgcttttca aagacgggtt
     2401 atagatgttt tttattaagt atatcgccat ttgtcgggca taagttatat gctgtttgcg
     2461 ggttgccgat tctaaatcac taatttgaac ctgataataa gcagcaactt ctttctttac
     2521 aagatcaaaa tagccgttgt tggtgctttt gtccgcatca cgctccggta aaaagcgttc
     2581 taaagcgaca taaacatttt ctacagtaaa gggaatgttt aaagacacac aataggtaat
     2641 ataacggcgc aatgctcctt ctaaatcccg aatattgtta gtaaagtaat cagcaatgat
     2701 atcaaggaca tttgttggga catcacttgg attttcaatt aaaaacgaca gtttccgttt
     2761 taaaatattc atccgtaagg gcttatccgg caccttaatg tccactgaca aaccccaatt
     2821 aaagcgggat ttcaaacgca ccatcatatt tttaagttcg gttgttgagc gatccgaagt
     2881 aatgacaatt tgtttgttta agccgtggag agtttcaaaa actttgaaaa attcttcttg
     2941 tgtggcttgt ttaccctcta aaaactgaat atcgtcgact aacaaaacat cagcacggcg
     3001 atatttttgg taaaaagctt caatgttttc cttacctttt ttagtagaag tgtggatgaa
     3061 ataatcttcg gtaaattctt gagccgttgt gtaaaccaca ttggtattag ggtcgttatc
     3121 aaggatataa tgaccgatgg ccatcattaa atgagttttc cctaaaccga catcaccaaa
     3181 aatatacagg ggattataaa ttttgtcgtg tgtttccgca actttcatcg ctgataagaa
     3241 agcaaagcga ttactttcgc cggtttcgaa attatcaaaa gtatattcgg ctttcaaggg
     3301 gcgtttatta atggtatctt ttgtggtaat tgatactttt tcctgttcaa ccgcatcttc
     3361 cttcaagata aatttaaaac ggcatggggt tttggtaatg cttgcgcaaa tcctatttag
     3421 tttttctaaa tagaactttt caatgcgaaa cttaaccaaa gcattgggca caataataaa
     3481 tattaaattg ttttcggttt tgaaaacttc gttaacatcc gcaaaaagtt ctaaaaaatc
     3541 ggtttcatca agctcatttt tcaatgttaa caaagtttgt tgccaaagct tcaaactgtc
     3601 ttccataact gctccctaaa cttatttacc attaaataat aacacatatc aagcaaataa
     3661 taaagcgaaa aataaaaaaa catcaaaaaa gaaacttatc cacttttcca ctgtttataa
     3721 gttgtggata acttatccac aaatggttaa tacatatcca aatagggaaa agtggaaaga
     3781 aaaacgaagt gggaacggta aaaaaacatt gcattaaaat gcgtttatga tacaatagaa
     3841 acaatgaaaa tatcaaagta gaggagaata accgctcggt tagtcaaaag catgaacaaa
     3901 gaaaaaaaca gtatcattta tcgaattttt aattttccta ataaaatcaa tgtgatcacc
     3961 atgtttgttg tcgtggtcgt ttatggcttg gtgcttttgt tgctggcgaa agtcatcact
     4021 ccggaagcgg attatgtggt agaactcaat tatccgcccg caacctacaa cagcgaaatt
     4081 aatccctaca tttttgtgcg tggcaccgcc atcaatgcaa gtgaagatga aaacaaaatc
     4141 gatttacgaa tgcaatatcg cattattgct tacattaata agatgaacac cccctaccca
     4201 actaatatta cttatgctta cagcgcgatg gataagggcg ggatgatgaa ctacttccta
     4261 gaagctaaaa gatcaagcct aaatgtctac caccaagtag tcgccagtgc cgaataccca
     4321 aaaggcgatt ggtatgagca atattttgta aaggtgcgct acgacattaa cgatggcaac
     4381 gaaaccgttc ataaaaccct aagcttatcg gaaaaagcct taaatttaaa aaagtcggaa
     4441 ttaaatgacc gtaaatttca aacttcacct aaattgccgg gcattttaga ggtaaaattc
     4501 atctttacgg atgacggcgc aaccattcca caatatgaca ccaatattca aattaccttt
     4561 gatgaaaaaa ctccctacca tttaaacttc cagtcctgga ttgtgaccga agggaaagag
     4621 attttccctt ttatcggtgt ttacaactat tgtacgccgg aaccccttaa accggcctac
     4681 gaaacatccg tttttaaatt tgtaaagcca aaaacgattt atatgaaatt agattacacg
     4741 gacgaaaaca atgtcctaac ccctttctat tataaagtcg atgtggcgac attattaaat
     4801 tccggaaatt aaaaccaacc aaaa
//
LOCUS       DAOSGN010000014         4772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_15962_length_4772_cov_2.781005, whole genome shotgun sequence.
ACCESSION   DAOSGN010000014 DAOSGN010000000
VERSION     DAOSGN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15962_length_4772_cov_2.781005"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PK087_00500"
     CDS             complement(<1..482)
                     /locus_tag="PK087_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HOH17777.1"
                     /translation="MDISLFLAFLRQHQLPFFPSFMYVVVKALNTIPEFRLRIQNGQV
                     IEYDIIHPAFTVMTDTGIYDNCDVEWQAFKEYLIAVKAKMAATKKGDISSFHDLMDQR
                     MDQFFISSIPWINFTSAIHPMAGNDFDSVPRILWGKHYEENGKTLIALQIQAHHALID
                     G"
     gene            complement(583..1671)
                     /locus_tag="PK087_00505"
     CDS             complement(583..1671)
                     /locus_tag="PK087_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOH17778.1"
                     /translation="MEAKVFFTSMRIRNRESLLDRLENLVKTAGLEQIDFQNKFVAIK
                     VHFGEPGNLAYLRPNYARRMVSVIKSLGGNPFLSDCNTLYVGRRKNALEHLSVAMENG
                     YNYFQTDCHVIIGDGLRGTDEALIDINGEFVKQAKVGRAFADSDIIISMNHVKGHGLA
                     GFGGALKNLGMGCASRAGKHEMHSSGKPFINSQKCRSCLSCLKNCAVKAISLRDGKAW
                     IDHNLCVGCGRCIGVCNFDAVQVDWNESSVKMNQKIAEYALAVVKGKPHFHINFVIDV
                     SPDCDCVASNNVPIVNNIGFLASFDPVALDQAASDLINQAPMINDYPSNHQDHFQSLN
                     PYTSWAAGLSHGEKIGLGSRTYQLIEVK"
     gene            1786..3282
                     /locus_tag="PK087_00510"
     CDS             1786..3282
                     /locus_tag="PK087_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOH17779.1"
                     /translation="MFPKPRYQKKNPGWFLLSPKQSINYAQVFSEEIKPLVLFWEQKW
                     QTPLLFSSFSTFNLLLDFHLQDEQYHLSITSEQITISAKDKQGMAYGIATFLALIKEE
                     NKQVFVEAQEIDDFPAFSYRGLMLDVARHFFPITTIKKVIDVMAQLKLNVFHWHFSDD
                     QGWRIESKVFPKLHENQSNFPASTKGYYSQEEIIDLVAYAAQKNITVVPEIDLPGHAG
                     AILAAYPHLSHHEQLAIRTTYGIAKEIMCLGNEQLLSFLQTLIEEMISLFQPRYFHLG
                     SDEVKLGECKKCKHCQQALKKHQLPHFLSLQTHLIKQLSQFLKSKKIIPLVWNDIAGE
                     VLDASLIIQHWKPFTWKRTALAQSRGHQIILSPFFNYYLDYPYSMTPLKKVYSYNPLL
                     NKQIRSHAILGLEACLWTEWIATEDKLFFHMFPRLAALADSAWRGFDQKDYREFCDSL
                     AFFEQWLERQQIPYAKGHYQQANIFVRILRTIKWLKNKQIEYEKQQED"
     gene            complement(3300..4034)
                     /locus_tag="PK087_00515"
     CDS             complement(3300..4034)
                     /locus_tag="PK087_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH17780.1"
                     /translation="MGRAHEVRAASMTKTAAAKSKLNAKFSMLIYRAAKTGIPDPEVN
                     QNLKKEIEKAKKAQIPADLIKRTIEKAKGGADDAYTEIRYEGFGPGNSQIIVECLTDN
                     VNRTYTAVRTILSKTGCKMGVPGSVTHMFSNQAIFSFEGFTDEETLDLLVAADCDVTD
                     IQIDEGMVSVFAPSTEYSKIKAALSEANPEIEFLEDKLAWIPMSYITLSEEDVPSMQR
                     VLTLLNEEDDVQEVFHNVEGLPALEE"
     gene            complement(4095..>4772)
                     /locus_tag="PK087_00520"
     CDS             complement(4095..>4772)
                     /locus_tag="PK087_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17781.1"
                     /translation="SSLIGTLGEKTIHRLVKTYLEPDGQFHEIKIGSYYADIYRDGHI
                     TEIQTGNFNKLRAKLAAFLPAYIVEVVYPSYARKWLRWQDESDGSLSKPRLSPSRGTY
                     YRVFSELYKIKAFLVHPHFRLRIILLDWEEHRYLNGWSLDRKKGSLRKECLPLAIVSE
                     VAIPSPADYIKLVPVALKSPFTTRDFAKAGSLSLKQAQKAVLVLAFLNIIQLMGKQGR
                     FHLYEIN"
BASE COUNT         1463 a         1052 c          870 g         1387 t
ORIGIN      
        1 ccatcgatta aagcatggtg agcttgaatt tgtaaagcaa tcagggtttt cccgttttct
       61 tcatagtgtt tcccccataa aatccgggga acggaatcga aatcattccc ggccatcgga
      121 tggatggcgg aagtgaaatt aatccacgga atgctactga taaagaattg atccatcctt
      181 tggtccatta aatcatgaaa agaagaaata tctccctttt tggtcgccgc catttttgct
      241 ttaacggcaa ttaaatattc cttaaacgct tgccactcca catcgcagtt atcataaatg
      301 cccgtatccg tcattaccgt aaaggcggga tggatgatat catattcaat aacttggccg
      361 ttttggatgc gtaagcgaaa ttcgggaatc gtatttaagg ctttcaccac tacatacatg
      421 aaactgggaa aaaacggcaa ttggtgttga cgaagaaaag ccaagaaaag acttatgtcc
      481 atcgtcgttg tcgccccata ataaggagct tggtattgag aaaaccatta aaaatgcttt
      541 tttcccgccc atgtttctaa atcaataatc cgttttccca ttttatttga cctcaatcaa
      601 ttgataagtg cgagaaccta aaccgatttt ttcaccgtgg cttaaaccgg ccgcccaaga
      661 agtataggga ttaagacttt gaaagtgatc ctgatgatta cttggatagt cattaatcat
      721 cggggcttga ttgatgagat cggaggcggc ttggtcaaga gccaccgggt caaaagaagc
      781 taaaaaacca atgttgttaa caatgggaac attgttgctg gctacacaat cacaatccgg
      841 agaaacatcg atgacgaagt tgatatgaaa atggggtttt cctttaacga ccgctagggc
      901 atactcggca atcttttgat tcattttgac actactttcg ttccaatcaa cttgaaccgc
      961 atcaaaatta caaacaccaa tgcagcgccc acaaccaaca cagagattgt gatcaatcca
     1021 cgctttgcca tcacgcaagg aaatggcttt aacggcacaa ttcttaagac aggataaaca
     1081 agagcggcat ttttgactat ttataaaggg ttttcccgac gaatgcattt catgctttcc
     1141 ggctcgcgaa gcacagccca ttcccaagtt tttgagggcg cccccaaaac cggccaaacc
     1201 atgacccttg acatgattca tcgaaataat gatatcactg tctgcaaagg cccgtcctac
     1261 tttagcttgc ttcacaaatt cgccattaat gtcaattaac gcttcatcgg tgccgcgcaa
     1321 accatcacca atgatgacat ggcaatcggt ttgaaaataa ttataaccgt tttccatcgc
     1381 gacgctcaaa tgttccaaag catttttccg gcgaccaaca tataaagtat tacaatcgct
     1441 taaaaaaggg ttcccaccca agcttttaat aacgctaacc atccggcggg cataattagg
     1501 tcgtaaatat gctaagtttc ccggctcgcc aaaatgaact ttgatggcca caaatttatt
     1561 ttgaaaatcg atttgttcca agccggccgt tttaactaag ttttccaagc gatccaataa
     1621 actttcacgg tttctaatgc gcattgatgt gaaaaaaact tttgcttcca taaaacacct
     1681 ctttttatat tataaacgaa agcaaataat atctcaagaa aaaattgacc acagctagcc
     1741 tttacatgtt gattgatttt cgctataata aaagcgaggt gccttatgtt tcctaaaccc
     1801 cgttaccaaa aaaagaaccc cggatggttt ttgttaagcc ccaaacaaag cattaattat
     1861 gctcaagtgt tttccgaaga aataaaaccc ctcgtcctgt tttgggagca aaagtggcaa
     1921 acgccactcc ttttttcatc gtttagcact tttaatctcc tcttagattt tcacttgcag
     1981 gatgaacaat accatctttc tatcaccagt gagcaaataa cgattagtgc caaagacaaa
     2041 caaggcatgg cctatggcat cgccaccttt ttagctttaa ttaaagaaga aaataagcaa
     2101 gtttttgttg aggcccaaga aattgatgac ttccccgcct tttcctaccg cggtttaatg
     2161 ttagatgtgg cccgccattt tttccccatc actaccatca aaaaagtcat cgatgtgatg
     2221 gcccaactaa aactaaatgt ctttcattgg catttctccg atgaccaagg ctggcggatt
     2281 gaaagcaagg ttttccccaa acttcatgaa aaccaaagca acttcccggc aagcaccaag
     2341 ggctattata gccaagaaga aattattgac cttgtggcct atgcggcgca gaaaaacatc
     2401 accgttgtcc cggaaattga tcttcccgga catgcggggg cgattttagc ggcttacccc
     2461 catttatcac accatgaaca attagccatc agaaccactt atgggattgc gaaagaaata
     2521 atgtgcttag gaaatgaaca actattaagc tttcttcaaa ctttaattga agaaatgatt
     2581 agcctctttc aaccccgtta ttttcattta ggaagtgatg aagttaaact aggagaatgt
     2641 aaaaaatgta aacactgcca acaagcgcta aaaaaacacc agcttccgca ttttttaagt
     2701 ttacaaaccc atttaattaa gcaactaagt caatttttaa aaagcaaaaa aattatcccc
     2761 cttgtttgga acgatattgc gggtgaagtg cttgatgcta gtctcattat ccaacattgg
     2821 aaacctttta cttggaaaag gacggcttta gctcaaagtc ggggccatca aatcatccta
     2881 tctccgttct ttaattatta tctagattat ccctatagca tgaccccgtt aaaaaaagtt
     2941 tattcttata atcccctatt aaacaaacaa ataagaagcc atgccatcct cggactggaa
     3001 gcttgtttat ggacggaatg gattgctacg gaagataaat tattctttca catgttcccc
     3061 cgcttagcgg ctttggcgga ttccgcatgg cgcggctttg accaaaaaga ttatcgtgaa
     3121 ttttgtgatt ccctagcttt cttcgaacaa tggcttgagc gtcaacaaat tccttatgcg
     3181 aaaggccatt atcaacaagc aaatatattt gttagaatct taagaacgat aaagtggctt
     3241 aaaaacaaac aaattgaata tgaaaagcaa caagaggact aattttagtc ctcttgctgt
     3301 tattcttcca aagccggtaa tccttccaca ttatgaaaga cttcctgaac atcatcttct
     3361 tcattaagta aagtcaaaac tctttgcatt gacgggacat cttcttccga taaagtaata
     3421 tatgacatcg gaatccaagc caatttatcc tctaaaaatt caatttccgg attggcttca
     3481 ctaagagccg ctttgatttt cgaatattcc gtcgagggtg caaaaacact aaccatcccc
     3541 tcatcaattt gaatgtcggt gacatcacaa tcggcagcaa ctaacaaatc caatgtttct
     3601 tcgtccgtaa aaccttcaaa gctaaaaatg gcttgattgc taaacatgtg agtgacactg
     3661 ccgggaacac ccattttgca ccccgttttc gatagaatag ttcgaacggc ggtataggtt
     3721 cggttaacat tatccgttaa gcattcgaca ataatttgtg aattaccggg accaaagcct
     3781 tcatatctga tttctgtata agcgtcatcc gccccacctt tggctttttc aattgttctt
     3841 ttaattaaat cagccggaat ttgtgctttt tttgcttttt caatttcttt ttttaagttt
     3901 tgattgactt caggatcagg gatccccgtt ttggcagctc gataaatgag catgctaaat
     3961 tttgcattca atttactttt ggcggccgct gtttttgtca tggaagcggc ccgaacttca
     4021 tgtgctcttc ccatattact atctcctttt ccttatctat tatacttctt tatcatccat
     4081 ttttccacaa attcttagtt gatttcataa agatggaaac gcccttgttt tcccatcaac
     4141 tgaatgatgt ttaaaaaagc caaaactaaa accgcttttt gggcttgctt taaacttaaa
     4201 gagccggctt tcgcaaaatc acgggttgta aagggggact tcaaagccac aggaactaat
     4261 ttaatgtagt cagcaggcga aggaattgcc acttccgaaa caatcgctaa gggtaagcat
     4321 tctttacgaa gcgagccttt tttacggtca agactccaac cattaagata acggtgttct
     4381 tcccaatcaa gtaaaataat ccgtaagcga aaatggggat ggactaaaaa agctttgatt
     4441 ttgtagagtt ccgaaaaaac gcgataataa gtcccccggc ttggtgacaa gcgtggtttg
     4501 gataaagagc catcactttc atcctgccag cgtaaccatt tcctagcata actgggataa
     4561 acgacttcca caatataagc cggtaaaaag gctgccagtt tagcgcggag cttattaaaa
     4621 ttcccggttt gaatttcggt aatgtgacca tcgcgataaa tatccgcata gtaagaaccg
     4681 attttaattt catgaaattg gccatcaggt tctaaataag tcttcacaag tcgatggatg
     4741 gttttttccc ccaaagtccc aattaaggaa ct
//
LOCUS       DAOSGN010000015         4760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_16019_length_4760_cov_4.394261, whole genome shotgun sequence.
ACCESSION   DAOSGN010000015 DAOSGN010000000
VERSION     DAOSGN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16019_length_4760_cov_4.394261"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK087_00525"
     CDS             <1..205
                     /locus_tag="PK087_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17782.1"
                     /translation="DIKKVEEGQINITTFDNIRKVTVDRAYTLKKHRPLYVGLKNIFL
                     LLSFGGSLTVVGYALYKIVVSLF"
     gene            215..4504
                     /locus_tag="PK087_00530"
     CDS             215..4504
                     /locus_tag="PK087_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17783.1"
                     /translation="METNPKKTPVSSPKKRSVKSGETKKTVVPVEEKPVDVVERLKNV
                     LAEVQENKEKPVPKKAAPVKKKEPAKKKTTKASSKTTAKKPASAKKKSEAKKRPTASL
                     EELNVKSKKPLPISKKSEQTPSVTSDVERLKGVISAGKRLQQLMEIKELKTESKERVL
                     KEKISLAQGLEEDLDEVTFDELEQEQSKVQELNEKIALLQAEKEKLDAAISKVMAAKV
                     SGIPIIDEESLDLSPSFPSGVPANQQEETSPTSKKISEIEEVLISLEKQHAENQIKLM
                     AAEQANKGLMDQYGEQAVVVNDLEKEVVALRGEVLNEQATNQQIKDQLEIELQAKEQY
                     QKEAEELKKQLDLSKAKFPTLKAALIEEMDDKIAIVEQRFLNVKKEGTRLKRELQSLT
                     KSVEKAQAKEELAKNKMLEEKKEKQTLAKEVASLKSNIAKMEASAETQMQKQQAVLQE
                     AKETKQAQTALKREITRLEKAVETQKTKEQQTRALLQDEKKANRRLQKDLDNLQIAFE
                     KEKEKAIPTEKIVAIDQAFQDEKKENARLQKSLNSLTDSFEKLKAQKGTKKAELDAVK
                     KEAARLNKDNEALKATFDDLKNKSLENKKLYDAEKKLTAKLEKRLENLTKSKKELQTT
                     IRYFKKQEQAQLEKDAQKDQLIEAKKAEKEQVEKQLEQLRQEVDGMMANLQTLHQTNE
                     TLKSRDYEENSLFEEENQQLRSDKQKIAEENNRLYDVIEDLRKTNDQLKNHNLELTKR
                     LETELGSLNQSLNSQAQENTALLAQLNTLENEKAQLEAELKEQMLRISETDEQDLPET
                     KQLKDANQQLIAANNDLNLALELLKTKYADLEKVLEKERLEKSQMLPSKDDSSKVAGL
                     INELNRTQDLVKAKDLEVEILKSALFREQNRVVSPEPYPFYNYDHQRYQQNILYQQPV
                     EPKIAPGLVEQQEKQSLVEEIKALKAEIDKLKQTPSVDSQALLNEFREELLRNREELS
                     RMNVQKEQEIQAIAENYETRLQSTEQEKVELKEQNNQRLQQISDLQTQVDDKEYTLQR
                     LKTTVKQFTEEDIFDPEFKRRIRLIRDMQKEIKNRLDEEAINYNSSMASLELKISQRQ
                     EDIDKLNSRINQLTATFNANRDFTASTKEAFEKAKAKALLELQLQEERLGGLEEDQAH
                     LKTKYQNFVSAKEQELTNLKIKEGQIIDYYLRKIRQDYALTDDYKEIKQVESERNQLL
                     TQIQELKASQEQTTETIVKQSEEISKVVAANQKLSAQLQDERKVAIDKQAGLLEAELR
                     EQGRELGEITRKMSDLKTELDKRLEYEKHLRINEEKVSDFFNNKILLEQCLTDFNARA
                     SQMTEVTARIESLGTEPANRTELLKAKAELTDLEVHRDDLRSKIDFCRKNLKDLETNA
                     TVATYMKLINQIDQIRNAQKELREKAELLKDSIQVKNKELDALMKEKAEIV"
     gene            4501..>4760
                     /locus_tag="PK087_00535"
     CDS             4501..>4760
                     /locus_tag="PK087_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17784.1"
                     /translation="MIITVDKSILRIIPCFYVIALTMDVTVRDSEGMQDIISQYEQKI
                     AEEYSLEEVLNIPLIKEARDGYKKFGKDSSRYRLAVESLYRR"
BASE COUNT         1882 a          830 c          967 g         1081 t
ORIGIN      
        1 ggatattaaa aaagtggaag agggtcaaat caatatcacg accttcgata atatccgcaa
       61 ggtgacggtt gaccgtgcct atacattaaa aaaacaccgg cccctttatg tcggtctcaa
      121 aaatatcttc ttattattaa gcttcggcgg ttcattaacc gtcgtcggtt atgccttata
      181 caaaattgtt gttagtcttt tttaggagga aaatatggaa actaatccca aaaaaacccc
      241 agtgagtagc cccaaaaaac gctctgtgaa aagtggggaa actaaaaaaa ccgttgttcc
      301 cgttgaggaa aaacctgtgg atgttgtgga acgtttaaaa aatgttttgg ccgaagtgca
      361 ggaaaataag gaaaaacccg ttcctaaaaa agccgctccg gttaaaaaga aggaaccggc
      421 gaagaaaaaa accaccaaag ccagttccaa aacaacagcc aaaaaaccag ccagtgctaa
      481 aaagaagagt gaagctaaaa aaaggcccac tgcttccttg gaggaattaa atgttaagag
      541 caaaaagcct ttgccgattt cgaaaaaaag tgaacaaacg ccgtccgtaa cgagcgatgt
      601 ggaacgctta aaaggcgtta tttcggctgg caagcgtctc caacaactaa tggaaattaa
      661 agaacttaaa accgaaagca aagaacgggt tttaaaagaa aaaataagtt tagcacaagg
      721 tttagaggaa gatctggatg aagtcacttt cgacgaactt gaacaagaac aaagcaaagt
      781 tcaagagctg aacgaaaaaa ttgcccttct ccaagctgag aaagaaaaac ttgatgccgc
      841 catttctaaa gtgatggctg ctaaagtaag cggcattccg attattgacg aagaaagcct
      901 tgacttgtct cccagttttc cttcaggcgt tccagctaat caacaagaag aaacttcgcc
      961 aacttctaaa aaaatatctg agatcgagga agtattaatc tcgctcgaaa aacagcatgc
     1021 ggagaatcaa attaagttaa tggctgccga acaagccaat aaagggttaa tggaccaata
     1081 tggtgagcaa gcggttgttg tcaatgattt ggaaaaggaa gtggttgctc tacgaggaga
     1141 ggtgctcaac gagcaagcca caaaccaaca gattaaagac caactggaaa tagaattaca
     1201 agctaaagaa caatatcaaa aagaggctga ggaattaaag aaacaactag atttatcaaa
     1261 agctaaattt ccgaccttga aagctgcttt aattgaagaa atggacgata aaatagcaat
     1321 agtcgaacaa cgctttctaa atgtgaaaaa agaaggaacg cgcttaaaaa gggaattaca
     1381 aagcctaacg aaaagtgttg aaaaagcgca agcgaaagag gaattggcta aaaacaaaat
     1441 gcttgaagaa aagaaagaaa aacaaaccct agctaaagaa gttgcaagct taaaaagcaa
     1501 tattgcaaaa atggaagcga gtgcagaaac acaaatgcaa aaacaacaag ccgtcctcca
     1561 agaggccaaa gaaactaagc aagcccaaac cgctttaaaa cgggaaatca cccgtttgga
     1621 aaaagcggtg gaaacgcaaa aaaccaaaga acaacaaacg cgggcattat tacaagatga
     1681 gaagaaggct aaccggcgct tacaaaagga tcttgataat ttacaaatcg cttttgaaaa
     1741 agaaaaggag aaagccatcc cgacggaaaa aatcgttgcc atcgatcaag ccttccaaga
     1801 cgagaagaag gaaaacgctc gcttacaaaa gtctttaaat tcgctgacgg actcttttga
     1861 gaaattaaaa gctcaaaaag gcaccaaaaa agccgaacta gatgcagtaa aaaaagaagc
     1921 ggcgcgctta aataaggata atgaagcttt gaaagccact tttgatgatt tgaaaaacaa
     1981 gagtttggaa aacaaaaaat tatatgatgc cgaaaagaag ttgacagcga aactcgaaaa
     2041 acgtttggaa aacttgacca agagtaaaaa agaattacaa accaccatcc gttatttcaa
     2101 gaagcaagaa caagctcaat tggaaaaaga tgcccaaaag gatcaattaa ttgaagccaa
     2161 aaaagcggaa aaagaacaag ttgaaaaaca acttgaacaa ttacgtcaag aagttgacgg
     2221 catgatggcc aatttgcaaa ctcttcacca aactaatgaa actttgaaat cgcgtgatta
     2281 tgaggaaaat tccttgtttg aagaagaaaa tcaacaattg cggagcgata agcaaaaaat
     2341 agcggaagaa aacaatcgtt tgtatgatgt gattgaggat ttaagaaaaa ccaatgacca
     2401 attgaaaaat cacaatttag aattaactaa acgcttggaa acagaacttg gcagtttaaa
     2461 tcaaagttta aacagtcaag cccaagaaaa taccgcttta ttggcgcaat taaacacctt
     2521 ggaaaatgag aaagcacaat tggaagctga acttaaagaa caaatgcttc gcattagtga
     2581 aactgacgaa caggatttgc cagaaaccaa gcaattaaaa gatgccaacc aacaattaat
     2641 agccgctaat aatgatctta atttagcact ggaactttta aaaacgaaat atgccgattt
     2701 agagaaagta ttagaaaaag aacgcttaga aaagagtcaa atgcttccaa gtaaggatga
     2761 ttcgtctaaa gttgccggct taatcaatga gttaaataga acgcaagatt tagtgaaagc
     2821 gaaagacctt gaagtagaaa ttttgaaatc cgctttattc cgtgaacaaa atcgggtggt
     2881 gtcgcctgaa ccttatccgt tctacaatta tgatcatcaa cgctatcaac aaaacattct
     2941 ttatcagcaa ccggtagagc ctaaaattgc gcctggcctt gtcgaacaac aagaaaagca
     3001 atctttagtt gaagaaatta aagctttgaa agcggaaatt gacaaattga aacaaacacc
     3061 gtctgttgat tcccaagccc ttttgaacga attccgcgaa gaactgcttc gtaatcgtga
     3121 agaattaagt cgcatgaatg ttcaaaaaga acaagagatt caagcgattg cggaaaatta
     3181 cgaaacccgc ttacaaagca cggaacagga aaaagttgaa ttaaaagaac aaaataatca
     3241 acgtttgcaa caaatcagtg atttgcaaac gcaagttgat gataaagaat atactttaca
     3301 aagattgaag actaccgtta aacagtttac tgaagaagat atctttgatc cggaatttaa
     3361 acgtcgcatt cgtttgattc gtgatatgca aaaagagatt aaaaatcgtt tggatgaaga
     3421 agccattaat tataattcaa gcatggcgtc cttagaactg aagatttccc aacggcaaga
     3481 agacattgat aaacttaata gccgcattaa tcaattaacg gcgaccttta atgccaaccg
     3541 tgattttacc gcctctacaa aagaagcgtt tgaaaaagcc aaagcgaaag ccttattgga
     3601 actgcaatta caagaagaac gtttgggtgg tttggaggaa gatcaagcac atttaaaaac
     3661 gaaatatcaa aattttgtta gtgcgaagga acaagaattg accaatttga aaatcaaaga
     3721 aggacagatt attgattatt atctccgaaa gattcgccaa gattatgctt taaccgatga
     3781 ttacaaagaa atcaaacaag tggaaagcga acgcaatcag ttattaacgc aaattcaaga
     3841 attaaaggcc tcccaagaac aaactacgga aacgattgtc aagcaaagcg aagaaatttc
     3901 caaagtggtg gctgctaatc aaaaactaag tgcccaatta caagatgaac gtaaagtcgc
     3961 gattgacaaa caagcgggcc ttttggaagc ggaattacgc gaacaaggtc gtgaattagg
     4021 cgaaattacc cgcaaaatga gcgatttaaa aactgaatta gacaagcgtt tagaatatga
     4081 aaagcatttg cgtattaatg aagaaaaagt gtcggatttc tttaataaca aaatcttatt
     4141 agagcagtgt ttgacagatt ttaatgctcg ggccagccaa atgactgaag ttacagcccg
     4201 gattgagagt ttgggtacgg aaccggccaa ccgcacggag cttttaaaag cgaaagcgga
     4261 attaacggat ttagaagtcc atcgtgatga tttgcgttcg aaaattgatt tctgtcgcaa
     4321 aaacttaaaa gatttagaaa ctaatgcgac cgttgccact tatatgaaac tgattaatca
     4381 aattgaccaa attcgtaatg cgcaaaaaga attacgggaa aaggccgaat tactcaaaga
     4441 cagtattcaa gttaagaata aagaactcga tgctttaatg aaggaaaagg ccgaaatcgt
     4501 atgattatta cggtcgacaa atcaattttg cgaattattc cttgttttta tgtcatcgct
     4561 ctaacaatgg atgttactgt tcgtgatagc gaaggcatgc aagatatcat ttcgcaatat
     4621 gaacaaaaaa tagctgagga atattcatta gaggaagtct tgaatattcc tcttattaaa
     4681 gaagccagag atggctataa aaaatttggc aaagattcga gtcgatatcg tttggcggtc
     4741 gaatctttgt atcgccggat
//
LOCUS       DAOSGN010000016         4758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_16030_length_4758_cov_3.460344, whole genome shotgun sequence.
ACCESSION   DAOSGN010000016 DAOSGN010000000
VERSION     DAOSGN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16030_length_4758_cov_3.460344"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PK087_00540"
     CDS             complement(<1..698)
                     /locus_tag="PK087_00540"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HOH17785.1"
                     /translation="MNNLNLEIFEQLVNLHAVAGHEKSVRQFMKQELSKYSDEIIQDK
                     LGSIFGVKKGLDKPIIMIAGHMDEVGAMVTAITDRGFIKMIPIGGLNPEVMLSQNVLV
                     NVGKERFVPGVVGAVPPHITKGEEHKSLSFDDLLLDVGADSKDHAQELGLEIGQQITF
                     MNQYYFSADGKKIVSKAWDNRFGCGMALEVLRELQTMDHPNTVIAGGTVQEEVGLRGA
                     TTASQMVEPDLFIA"
     gene            complement(695..1330)
                     /gene="trmB"
                     /locus_tag="PK087_00545"
     CDS             complement(695..1330)
                     /gene="trmB"
                     /locus_tag="PK087_00545"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOH17786.1"
                     /translation="MRLRKIKQADVKLTAFFDIVILGGEQNRGHWQDVFANNHPIYLE
                     IGMGKGQFISEHARRHPDINYIGLEKYDSIVLKAAQRLEKEFLPNLRLLRADAKNLEL
                     IFQPGEITTIFLNFSDPWPKERHAKRRLTFDNFLRTYEQILSRPGIIELKTDNRHLFE
                     YSLLKFNEHGYRLLAINLHLHEQANPDVITTEYEDKFINENKIIYYMKVEL"
     gene            complement(1331..2665)
                     /gene="pepV"
                     /locus_tag="PK087_00550"
     CDS             complement(1331..2665)
                     /gene="pepV"
                     /locus_tag="PK087_00550"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HOH17787.1"
                     /translation="MNLVEIAKRYEQDFIKDTQGLLQIDSTLIEQPKNSKHPFGEGIY
                     RSLEYMLKLGASFGFKTKEVDHIAGHIEFGEGEEIIGVLVHVDVVPAVGDWLYPPFSG
                     TVADGKIYARGALDDKGPAVCALYAFKLLKDLGFKPNKRLRLIIGTDEETSWRGIKRY
                     FEKEEMPLFGFSPDANFPLIYGEKGIMSLDLLGAGDPKLFFKAGVRYNVVPDYAEAKI
                     DQDVSEAFHAFLLEHHLKGQAKEILSLYGKSAHAMEPDAGINAAIKLSQFLQKYSSNQ
                     ALNFIAGQLKDSRFKTMGLDFQDPEMKDLTVNVAIVEINKEETKIGLNLRYPINWHKE
                     AFLQIFSQKAAAFGLKVLVKQDQIPHYVDKNDPLIKTLYQAYVRNTGDAKTPMMTIGG
                     GTYARALKKGVAFGPEFPHRENVVHQPNEYLIIEDAIKAMAIYADALRELSK"
     gene            complement(2740..4722)
                     /gene="tkt"
                     /locus_tag="PK087_00555"
     CDS             complement(2740..4722)
                     /gene="tkt"
                     /locus_tag="PK087_00555"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013488988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOH17788.1"
                     /translation="MNKDKLAIEALRILSVEQITRANSGHPGIALGAAPILHTLYSKH
                     LKVSPKHHQWINRDRFVMAAGHGSALLYALLYLADFGLDLDDLMQFRQFGSKTPGHPE
                     VHITPGVDASSGPLGQGIAMATGMAIGEEYLRARFNRPELPIIDHYTFVLCGDGDLQE
                     GVTQEAMSLAGHLGLSKLIVLFDSNDIQLDGKVANTNTEKASDKYPAMGWDYLFVSNG
                     EDLDELSEALGIAKKNSKPTIIEIKTIIGFNSSVANTAKAHGSPLSQEEVQKMRAEFG
                     GEAFSVPTEVWGYYQKNIVDSGKAQFEKWQQLWYRYQAEYPHEAQQLQNLDHWEIDEN
                     WDRLFNFDEQYKKATRATSGEILNHLQTLHPGLIGGSADLSSSTKVKGIDGDFSKTKR
                     LGRNLNFGVREHAMGAIANGLALHGGIRPFVGGFFVFSDYLKPAMRLSSLMDLPVIYI
                     FTHDSIAVGEDGPTHQPIEQLSQLRSTPHLNVIRPADANETLVAWDVIMKTQNNPVAL
                     ILTRQDVPNITNKKLANHLRQGAYVISPERKKLDGVFLASGSEVKLALDAQTELYKDG
                     LDVRVVSMPSFYLFEKAEQAYQEQVIPKDVKTKVAIEMSDAVHYYRYLGDNGLLINIE
                     TFGQSAPAGKLLSHYGFTVDNVVRLFKECWQKNHSK"
BASE COUNT         1393 a         1069 c          876 g         1420 t
ORIGIN      
        1 atggcaatga ataaatccgg ttccaccatt tggctggcgg tggtcgcccc ccttaaaccg
       61 acttcttctt ggacggtgcc cccggctata acggtattcg ggtgatccat cgtttgtaat
      121 tctcgtaaaa cttccaaggc catcccgcaa ccaaaacgat tatcccaagc tttggagacg
      181 atttttttac cgtcagccga aaaatagtac tgattcataa aagtaatttg ttggccgatt
      241 tcaagaccta attcttgggc atgatccttg gaatccgccc ccacatccaa taacaaatca
      301 tcaaaggata gggatttatg ttcttctcct ttagtgatat ggggcggaac agcacccacc
      361 accccgggaa caaagcgctc ttttcccaca ttgactaaga cattttgtga caacataacc
      421 tcgggattga ggcccccaat gggaatcatt ttgataaaac cgcggtcggt aatggcggta
      481 accatggctc cgacttcatc catatggccg gcaatcatga taattggttt atcgaggcct
      541 ttcttcaccc caaagatact tcccagttta tcttgaatga tttcatcgct gtatttgctt
      601 aattcttgtt tcataaattg gcgcacggat ttttcatgac cggcaacagc atgtaaattg
      661 actaattgtt caaaaatttc taaattgaga ttgttcataa ttccaccttc atataatata
      721 taattttatt ctcattaata aatttgtctt catattccgt tgtaatcaca tccggattag
      781 cttgttcatg taaatgaaga ttaatagcta gtaaacgata tccgtgctca ttaaacttca
      841 ataaactata ttcaaataaa tgacggttat cggttttcaa ctcaatgatg cccggacgac
      901 ttaaaatttg ttcgtaagtt cttaaaaaat tatcaaaagt taagcgtcgc ttcgcatggc
      961 gttctttcgg ccaaggatca ctgaaattaa gaaagatggt agtaatctca ccaggctgaa
     1021 aaattagttc caaatttttc gcatctgccc gcagcaagcg taaattaggt aaaaactctt
     1081 tctctaaacg ttgagcggct tttaaaacaa tgctgtcata tttttctaaa ccgatgtagt
     1141 taatgtcggg atgacgacgg gcatgttcac taatgaattg tccttttccc atgccaattt
     1201 ctaaataaat gggatggttg ttggcaaaaa catcctgcca atgtccccgg ttttgctcac
     1261 cacctaaaat gacgatatca aaaaaggcag ttaatttcac atcagcttgt ttaatctttc
     1321 ggaggcgcat ttatttgctt aattccctta aggcatccgc ataaatggcc atcgccttaa
     1381 tggcatcttc aattattaaa tattcattcg gttgatggac aacattttcc cgatgaggaa
     1441 attccggccc aaaagccacc cccttcttta aagcccgggc ataggttcct ccaccaatgg
     1501 tcatcatcgg ggttttggca tcgccagtat tacgaacata ggcttgatag agggttttaa
     1561 ttaggggatc atttttatca acataatggg ggatttgatc ttgtttcaca agcactttca
     1621 aaccgaaagc cgccgctttt tgactaaaaa tttgtaaaaa cgcttctttg tgccaattaa
     1681 tcggatagcg taaattcaac ccgattttcg tttcctcttt attaatttct acaatcgcca
     1741 cattgacggt taagtccttc atttccggat cttggaaatc caagcccatg gttttaaagc
     1801 gggaatcttt tagttgaccg gcaataaaat ttaaagcctg attacttgaa tacttttgga
     1861 gaaactgact taatttgatg gcagcattaa ttccggcatc gggttccatc gcatgggcac
     1921 ttttcccata aagcgataat atttctttag cttgaccttt taaatgatgt tctaaaagaa
     1981 aagcgtgaaa ggcctcggaa acatcttggt caattttagc ttcagcataa tcaggaacaa
     2041 cattatagcg aactcctgct ttaaaaaaca acttggggtc accagcaccc agtaaatcaa
     2101 gcgacataat tcctttttca ccataaatca aggggaaatt ggcatccgga gaaaaaccaa
     2161 acaggggcat ttcttctttt tcaaaataac gcttaatccc ccgccaagat gtttcttcat
     2221 cggtaccgat gattaaacgc aggcgtttgt tgggcttaaa gccgagatct ttcaataatt
     2281 taaaagcata gagcgcacag accgccggac ctttgtcatc aagcgcacca cgggcataaa
     2341 ttttcccatc agccaccgtt cctgaaaagg gcggatacaa ccaatcgccc accgccggga
     2401 ccacatcgac atgaactaaa accccaataa tttcttcacc ttcaccaaac tcaatatgac
     2461 cggcaatatg atcaacttct ttcgttttaa agccaaaact tgcgcctagc tttaacatgt
     2521 attccaaact tcgataaatt ccttccccaa aagggtgttt gctatttttt ggttgttcaa
     2581 ttaatgtgct gtcaatttgt aataatcctt gtgtgtcttt aataaagtct tgttcgtatc
     2641 ttttcgcaat ctccactaaa ttcattttct caccttttca ctgctactta attataccat
     2701 agctaaactc ttttccacta caaaaaaagc aagtgaattt cacttgctgt gatttttttg
     2761 ccagcattct ttaaaaaggc gcaccacatt atcaacggta aaaccataat gggaaagtaa
     2821 cttccccgcc ggcgcacttt ggccaaaagt ttcaatatta attaggagcc cattatcgcc
     2881 taaatagcgg taataatgga cagcatcact catttcaatg gcaactttgg ttttaacatc
     2941 tttagggata acttgttctt ggtaggcttg ttccgctttt tcaaataaat aaaacgacgg
     3001 catgctgacc acccgcacat ccaaaccatc tttatataat tcggtttgcg catctaaagc
     3061 gagtttaact tcggagccac tcgctaagaa aacaccatcg agtttttttc gttccggaga
     3121 aataacataa gctccttggc gaagatggtt agctaatttt ttgttcgtaa tattcggaac
     3181 atcctgtctc gttaaaatta atgccaccgg attattttgc gttttcataa taacatccca
     3241 agccactaag gtttcattgg catcggccgg acgaatgaca ttaagatgag gcgtacttcg
     3301 tagttggctc agttgctcaa tcggttgatg agttgggcca tcttcaccga ccgcaatcga
     3361 atcatgggta aagatataaa taaccggtaa atccatcaat gaagaaagtc gcatagccgg
     3421 ttttaaataa tcggaaaaca caaaaaaacc accgacaaag ggcctaattc caccgtgcaa
     3481 agctaaaccg ttagcaatgg cacccatggc gtgttcacgg accccaaaat taagattgcg
     3541 ccccaaacgc ttggttttgg aaaaatcacc gtcaatgcct ttaacttttg ttgatgatga
     3601 aagatcggcc gaaccaccga ttaagcccgg gtgtagtgtt tgtaagtgat taagaatctc
     3661 gccactggtg gcccgggtag cttttttata ttgttcatca aaattaaata agcgatccca
     3721 attttcatca atctcccaat ggtctagatt ttgtaattgt tgggcttcgt gtggatattc
     3781 ggcttgatag cgataccata attgttgcca tttttcaaat tgggccttac ctgaatcaac
     3841 gatattcttt tgataatatc cccaaacttc ggtaggaaca ctaaacgctt caccaccaaa
     3901 ttcagcccgc attttttgca cttcctcttg acttagagga ctaccatggg ctttggcagt
     3961 atttgctacc gacgagttaa aaccgataat ggtctttatt tcaatgatgg tcggtttgct
     4021 gttctttttc gcaattccca aggcctctga aagctcatca aggtcttcac cgttactaac
     4081 aaataaataa tcccatccca tagcgggata tttatcggaa gctttttccg tattggtgtt
     4141 agcaactttc ccatcaagtt gaatatcatt ggaatcaaaa agaacgatta atttacttaa
     4201 tcctaaatgc cccgccaaac tcatcgcttc ttgggtgaca ccttcttgca aatcaccatc
     4261 accgcaaaga acaaaggtat agtgatcgat aatcggtaat tccgggcgat taaaacgggc
     4321 ccttaaatat tcttcaccaa tggccatacc ggtagccatg gcaatccctt gccctaaagg
     4381 accggacgaa gcatcaacac ccggagtaat atgaacttcc ggatggccgg gagtcttact
     4441 gccaaactgg cgaaattgca ttaaatcatc aaggtctaag ccaaaatcag ctaaatacaa
     4501 aagcgcatat aacaaagcag agccgtggcc ggcggccatc acaaagcggt cacgattaat
     4561 ccattgatga tgtttcggtg aaaccttcag atgtttgcta taaagcgtat gcagaattgg
     4621 ggctgctcca agagcaatgc cgggatgtcc ggaattggca cgcgtaattt gttcaacgga
     4681 taatattctt aaagcttcaa tggccaattt atctttattc atcgattttc gaaaaatcct
     4741 ttaaaatatt atagatgt
//
LOCUS       DAOSGN010000017         4535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_17146_length_4535_cov_3.334821, whole genome shotgun sequence.
ACCESSION   DAOSGN010000017 DAOSGN010000000
VERSION     DAOSGN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17146_length_4535_cov_3.334821"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..196
                     /gene="rpmH"
                     /locus_tag="PK087_00560"
     CDS             62..196
                     /gene="rpmH"
                     /locus_tag="PK087_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003148884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOH17789.1"
                     /translation="MKRTYQPNKRKHIKTHGFRARMATVGGRAILSRRRLKGRYRLTA
                     "
     gene            272..622
                     /gene="rnpA"
                     /locus_tag="PK087_00565"
     CDS             272..622
                     /gene="rnpA"
                     /locus_tag="PK087_00565"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOH17790.1"
                     /translation="MNQQHRLKKNHDIEKLIKLRQSVGNKYYAIFFAASKNPLAQVAI
                     SPSRKFKTAVAKNYEKRVVREIIRPLLSELSFLKLLIVVKQGAGELSFAQKQEQIIYL
                     LNKIKEQIINKEKK"
     gene            619..1527
                     /locus_tag="PK087_00570"
     CDS             619..1527
                     /locus_tag="PK087_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOH17791.1"
                     /translation="MKTNYKKYLFVALVIIGLFILSSCGGKPTEYKPIDGVKGIGDIL
                     VWPMAALMWLIGKSVAFGYYGIVILVATIIVRTLAWPIYAKTNDMTLKMQLMAPEQAK
                     IEQKYAGKTDPESNQRKQMELMQLYKKYGVGIGGCLIQFIQLPIFISFYQTLQRVPKT
                     VGAEYTFDFSFLNTNFFGINLFATRSESTYQMWGIIILAILVGGTQIVSQIIATRRQK
                     KAKAESQSNIPAYRQPAQTDIQKQTETTMKIMMYGMTAMMMVFVYNSTAALGFYWLVG
                     NLYSTLQGYFGHKSSQKRMARLKAKM"
     gene            1538..2206
                     /gene="jag"
                     /locus_tag="PK087_00575"
     CDS             1538..2206
                     /gene="jag"
                     /locus_tag="PK087_00575"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOH17792.1"
                     /translation="MNRKTYEVKTVEEAKQLAVADFGVKESELTFTIINEKKGFLGIG
                     SKIEVEAVLDADGITRGKEYIHMILSNNNIQGFIEKRVRGDNVEFNIDAGDFNGYLIG
                     KNSRNLIALQILVSAVVNKYYSEGEAKTVMVDVGGYRKRREKNLENMAVDFGKQVSRS
                     KQKIQLDYLNAYERKIIHNKLSSWKDVRTYSVGEEPNRFLIIEPRNTPKEAKPKTSET
                     KNQE"
     gene            complement(2452..3309)
                     /locus_tag="PK087_00580"
     CDS             complement(2452..3309)
                     /locus_tag="PK087_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOH17793.1"
                     /translation="MFGKRSDGVRLKGLDPIFKIIPHIMKARHDSQNSYLYPVLCDPL
                     DDFIAREKQNGVYYNYMHIMIAAVVRLIALRPQLNRFVMNGRHYKRKAIYVSFAVKKS
                     LKDEGQETTIKLVFSGLETLHEIKERIETAILENTNINTKNNTDKLAKLFTSLPNCLI
                     KLAVGFVKWLDKHGMLPKKVIEASPFHTSFFITNLKSIKIDFIYHHLYDFGTTGIFVS
                     MGKEKMEAVVQGDNEIVAKKVMNVGVVTDERFCDGLYYANSLRLLKRILENPSILAAG
                     LDKINQDIN"
     gene            complement(3384..4067)
                     /locus_tag="PK087_00585"
     CDS             complement(3384..4067)
                     /locus_tag="PK087_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOH17794.1"
                     /translation="MNDWPLVKMQELASLKFNSQQLEPGFKEQFIFNSLAYENDWVTI
                     DHVRDVLADQKTSLDAKKVFVINNFAKALTFTMNLFNLGQKLDENKLKDIHEILMDNL
                     GVGGLYRNVDISVQGSNHTPPSHVKVYDRMKKYFDALNNDHGDIFEKIAFSHLQLVKI
                     HPFLDGNGRCARLVLAFELLKHGLAPVIIPFSDRDKYFHMLEEFKVNKNILPFVNYLK
                     HLEQTYLAS"
     gene            4193..>4535
                     /locus_tag="PK087_00590"
     CDS             4193..>4535
                     /locus_tag="PK087_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17795.1"
                     /translation="MILFLKLVKRIMVVILLILIVFMINGLTERVFANQKLKAFKERG
                     ELIVTNDPNPRNHYFLVAPINDYEDISRPVFNMDLRLIGAKADVIVTNRNPMRHKPAI
                     GWATGLLSRSLF"
BASE COUNT         1520 a          802 c          886 g         1327 t
ORIGIN      
        1 tgacttaagg gataaaatat aatataataa ccaagcatgg tttaatatag gaggtgtccg
       61 tatgaaaaga acataccaac cgaataaacg caaacatatc aagacccatg ggtttcgtgc
      121 ccgaatggcg actgtcggtg gcagagcaat cttatcccgt agacgtctta agggtcgtta
      181 ccgacttacc gcttaaacaa aaaaactagg accactcgat cgtcacaatc catcagagag
      241 gtcttttttt tgcttaagga agaggcatta tatgaatcaa caacaccgtt taaagaaaaa
      301 ccatgacatc gaaaaactga ttaagttgcg acaaagcgtc ggcaacaaat attatgccat
      361 tttttttgct gctagcaaaa atcctttagc gcaagtagcg atttcaccat caagaaaatt
      421 taaaacggct gttgcgaaaa actatgaaaa acgagttgta cgcgaaatca ttcgcccgct
      481 tctttcggaa ctttcgtttt taaaattatt aattgtggta aaacaaggcg ccggggaatt
      541 aagttttgct cagaaacaag agcaaataat ctatttactc aacaagatca aagaacaaat
      601 tattaataag gagaagaaat gaaaacaaac tacaaaaaat atttgtttgt ggctctagtc
      661 atcatcggct tatttatctt aagcagttgt ggtgggaaac ccaccgaata caaaccgatt
      721 gacggcgtta agggcatcgg cgatattctt gtctggccga tggccgcatt aatgtggttg
      781 attggaaaaa gcgttgcttt tggttattat gggattgtta ttttagtggc aaccattatt
      841 gtgagaaccc tagcttggcc aatttatgcg aaaacgaatg atatgacttt gaaaatgcaa
      901 ttgatggctc cggaacaagc caaaatcgaa caaaaatatg cgggtaaaac cgacccggaa
      961 agcaatcaac gaaaacaaat ggagttaatg cagctgtata agaaatatgg tgtcggtatt
     1021 ggcggctgtt taatccaatt tattcaatta ccaatcttta tttccttcta tcaaacactg
     1081 cagcgcgttc cgaaaacggt tggggcggaa tatacatttg atttttcgtt tttaaacacc
     1141 aatttttttg gaatcaactt atttgctacc cgcagtgaaa gtacttatca aatgtggggt
     1201 attatcatct tagccatttt ggttggggga acacaaattg tcagtcaaat aatcgccacc
     1261 cgccggcaga aaaaagccaa agcggaaagt cagtccaaca ttccggctta tcgtcaaccc
     1321 gcccaaaccg atattcaaaa acaaactgaa accacaatga aaatcatgat gtacgggatg
     1381 actgccatga tgatggtctt tgtttacaac agtacggccg ccctcggatt ttattggttg
     1441 gttggtaacc tttattccac attacaaggg tattttggcc acaaaagcag ccaaaaacgg
     1501 atggcccgtt taaaagcaaa aatgtaagag gtgtattatg aatagaaaaa catatgaagt
     1561 aaaaactgtt gaagaagcta agcaattagc ggttgccgat tttggtgtta aggagtccga
     1621 attgaccttt accattatta atgagaaaaa aggcttttta ggaatcggta gcaaaattga
     1681 agttgaagcc gttttggatg cggacggcat tacgcgagga aaagaatata ttcacatgat
     1741 tttaagcaac aacaacattc aaggctttat tgagaaaaga gtgcgtggcg ataatgtgga
     1801 attcaatatt gatgccggcg atttcaacgg ctatttaatt ggaaagaatt ctcgcaacct
     1861 cattgctttg caaatacttg tttcggcagt ggttaataag tattatagtg aaggcgaagc
     1921 taaaacagtg atggttgatg tcggcggata ccgcaaacgt cgcgaaaaaa acctagaaaa
     1981 tatggctgtt gattttggaa aacaagtttc ccgctctaaa caaaaaatcc aattggatta
     2041 tttaaacgct tatgaaagaa aaattatcca taataaacta tcatcttgga aggatgttcg
     2101 cacttacagt gttggagaag aaccgaatcg ttttttgatt attgaaccac ggaatacccc
     2161 aaaagaagca aaaccaaaga caagtgaaac gaaaaaccaa gaataagata ctgatatcta
     2221 tcaagaatta ttctaataaa agaggtctat gtttagtcgc acttatgtga aaaacataga
     2281 ccttttttgt attaagaaaa ttattaatgt tttttagttg tgtttaatat tagaattaat
     2341 aaagattaaa ccaattcttg gttaaaccta gccatttaca caacaaagaa agccattaaa
     2401 aaaactgtca agaattctgt tgattatcaa caaaagattt gatttgcttt tttagttaat
     2461 atcttggttg attttatcca gtccggcagc aaggatagaa gggttttcta agattctttt
     2521 gagcaatcgt aacgagttgg cgtagtataa accatcacaa aaacgttcat cggtaacaac
     2581 accgacattc atgacttttt tcgcaacaat ctcgttgtct ccttgaacaa cagcctccat
     2641 tttttctttc cccatactga caaaaatacc ggttgtacca aagtcatata aatgatggta
     2701 aatgaaatca attttaatgg attttagatt ggtgataaaa aagcttgtat gaaagggtga
     2761 ggcttcaatt accttcttcg ggagcatgcc atgcttatca agccacttca caaagcccac
     2821 ggccaactta attaaacaat taggaagcga agtaaagagt ttagcgagtt tatcagtatt
     2881 gttcttggtg ttgatattag tgttttctaa aatcgctgtt tcaatccgct ctttgatttc
     2941 atgaagagtt tctaagccgg aaaaaactaa cttaatagtc gtttcttggc cttcatcttt
     3001 taaactcttt ttcacggcaa aggaaacata aatggctttt cgtttataat ggcgaccatt
     3061 cataacaaag cgatttaact ggggacgtaa agcaattaac cttacaacag ccgcaatcat
     3121 gatgtgcata taattgtaat aaactccgtt ttgtttttcg cgagcaataa aatcatcaag
     3181 aggatcgcaa agaaccggat ataaataaga attttgtgaa tcatggcgcg ctttcatgat
     3241 atggggaatg attttaaaaa tcggatcaag ccctttaaga cgaacgccat cgctacgttt
     3301 accaaacata ctaaaacctc cgtgttcccc attttaacac aagttaaaac gaaagtaaaa
     3361 taataaaaaa agccctgagg gctttaagag gctaaatatg tttgttccaa atgtttaaga
     3421 taattaacaa aaggcaggat gtttttgtta actttaaact cttccaacat atggaaatat
     3481 ttgtcacgat cagaaaaagg aatgatgacg ggtgccaatc cgtgttttaa aagctcaaaa
     3541 gccaaaacca agcgggcaca acggccgttc ccatccagga agggatggat tttcacaagc
     3601 tgtaaatggc taaaagcaat tttttcaaaa atatcgccat ggtcgttgtt taaagcatca
     3661 aagtattttt tcatacgatc atagactttc acatggctgg ggggagtgtg gttggagcct
     3721 tgaacggaaa tgtcgacatt gcggtataag cctcccacac cgagattatc cattaagatt
     3781 tcatggatat ctttcagttt gttttcatct aacttttgtc ctaagttaaa caggttcatg
     3841 gtaaaggtca aagccttagc aaagttattg atgacgaaaa cttttttcgc atcaagcgat
     3901 gttttttggt cagcgagaac atcgcggaca tgatcaatcg ttacccaatc attttcataa
     3961 gctaaagagt tgaaaatgaa ttgttcttta aagccaggtt caagttgttg ggaattaaat
     4021 tttaacgatg ctaattcctg catcttaact agtggccagt cattcatctt tatacctcca
     4081 gataataatt taccctatta taccatagga ggggtcaatt aacaacaaca ataatattga
     4141 aataaaccgt gattttaagt ataatgaatg aaagaatgaa aagaggacga agatgatttt
     4201 gtttttaaaa ttggtcaaac gcatcatggt cgttatttta ttaatcttga ttgtttttat
     4261 gattaatggt ctaacggaga gagtgtttgc caatcaaaaa ctgaaggctt ttaaagagcg
     4321 cggcgaatta atcgtgacca atgatcccaa cccccgcaat cattactttc ttgtggcccc
     4381 tattaatgat tatgaagata tcagtcgccc tgtttttaat atggatttac gacttattgg
     4441 ggccaaagcc gatgtcattg ttacgaatcg caaccctatg cgtcataaac cggcgattgg
     4501 ctgggctacg ggcttgttgt cgcgaagttt atttt
//
LOCUS       DAOSGN010000018         4471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_17550_length_4471_cov_3.748641, whole genome shotgun sequence.
ACCESSION   DAOSGN010000018 DAOSGN010000000
VERSION     DAOSGN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17550_length_4471_cov_3.748641"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PK087_00595"
     CDS             <1..492
                     /locus_tag="PK087_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH17796.1"
                     /translation="YRRSVRSFTDQVIEPEKIEQLLRAGMQAPSARNQQPWHFLVVQN
                     REQLLKIYETSPYARMFTEATAAIIVLMDKTNLKNELMAPQDLSAATLNILLRATELG
                     IGSCWCGVFPNSERMASLKVLIKAPDQYEVFSLIALGYPQDADAFRFVDRFDPTKISY
                     EEF"
     gene            495..1325
                     /gene="mutM"
                     /locus_tag="PK087_00600"
     CDS             495..1325
                     /gene="mutM"
                     /locus_tag="PK087_00600"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017418340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOH17797.1"
                     /translation="MPELPEVETVRRTLATLVVGKTIADVSAPYPKIIKNLSFSHFRG
                     TLINETITDIKRKGKFLIVVFTKCVLVSHLRMEGKYFYKTTPAIEKHEHVIFWFTDGS
                     SLHYHDTRKFGTMYLFPTRDMTLITQLEPLSQVGLEPMDANMNVAYLKARFQKSTKAI
                     KTTLLDQKIMAGLGNIYVDEVLFLSKIHPETPTKTLSDEQIALIITNAQTVLTKAIAL
                     GGTTIRSFTSAHDVSGRFQNELLVHTKTECPVCQAKIKKIKVGGRGTYVCENCQKPLT
                     "
     gene            1370..1948
                     /gene="coaE"
                     /locus_tag="PK087_00605"
     CDS             1370..1948
                     /gene="coaE"
                     /locus_tag="PK087_00605"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOH17798.1"
                     /translation="MIVGITGSIASGKSLVTSYLAEKGYQIIDSDVIARAQLQKPEVI
                     TKLRAAFGKEIIQDEQINRRVLGKIIFQDEQKRQILNQILHPLIIQEIKHLIKDAKEL
                     VFVDVPLLYEAKMEALFDKIIVVWINRDQQLKRLMKRDRISEAYALLKINSQDDLESK
                     KARADFVIDNNGRPTHTQFQVEQILRRLKNEN"
     gene            1938..2309
                     /locus_tag="PK087_00610"
     CDS             1938..2309
                     /locus_tag="PK087_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17799.1"
                     /translation="MKIRDYFSNDFETGEDHYLPSLKTRYYRCRIEDVKAAILRFVEE
                     EKGVVKAIMDEHHEIFFVTPNYSSTITYINTRIGEAAIDIKITTYRLIPWGAGKKTIE
                     RMYKYFDAHLPFKGVSLYRGS"
     gene            2306..3583
                     /locus_tag="PK087_00615"
     CDS             2306..3583
                     /locus_tag="PK087_00615"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOH17800.1"
                     /translation="MMAISISDNFKITQKHALSGEDYFVLSQMYLPIIGIDSFSLYHL
                     LFTLNESDTYSIKKLVDFLSCSSPSFLEQALHKLEGVSLIDTYYHEHKGYLFNLKTPL
                     SRTSFLENSLLASFLSMQIGEVEMKKMQEQDKINVRQYKEISKAFDEVYDVSTDNVSN
                     LFKRLFKVKKNKGIKVINPDFDYIVFKMSFDTDFFDQKLLDDEDFKQHILSISYNYKL
                     NEEEMKEVIEKTISADRDLKYTDISKHAKAMFQLKHKQQRPRILTKTPDAFMQSQMDD
                     DYYRVFEVLENKTPSDILAELSGIQPSVAEVEMFQQLIDNTKFPISVINLMILYVNTL
                     KKGELPNYNYFEKLANVWARMGIKNVKDALDYLNQPKPTKEVKKGMYAKKEKPLPDWY
                     DQYTTQLSKKKENETLSEEEVNKILSEAEKKYN"
     gene            3592..4458
                     /locus_tag="PK087_00620"
     CDS             3592..4458
                     /locus_tag="PK087_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH17801.1"
                     /translation="MSNEFKKITKAIFEDKLLKEFVEKNKLSEEDILNNLSRFYIQKE
                     NNAICLPCDGSNCQMDPKAMQTRLVMEGNLDLVYFPCPKYEQAISDNLEMLFFSQPAL
                     FENQKLFINNERVFALKHMDMFLKKYQKGVFTKGLYLHGAFGTGKTFILWQLAKQLTK
                     KGVKVMMVYYPDLVRTIKSYIATSEFEPLINRLKYIDVLILDDVGAENNTAFIRDEVL
                     GPILQFRRDALLPVCMSSNYNFTLLRDHFSETKDEINHIKSGRILERIQSLMDSIELV
                     DKNYRAVDSNSK"
BASE COUNT         1462 a          790 c          882 g         1337 t
ORIGIN      
        1 tatcgacgta gtgtccgatc ttttaccgat caagttattg aaccggaaaa aatcgaacaa
       61 ttattaagag cggggatgca ggctcccagc gctcgtaacc aacagccttg gcatttttta
      121 gttgtccaaa accgcgagca attattgaaa atttatgaaa catcacccta tgcaagaatg
      181 ttcaccgaag ccactgccgc cattattgtt ttaatggata aaacgaattt gaaaaatgag
      241 cttatggccc cgcaagattt atcggcggct actttaaata ttttattaag agcgaccgaa
      301 ctgggaattg gcagttgctg gtgcggtgtt ttccctaatt cggaacggat ggccagtcta
      361 aaggtcctca ttaaggctcc ggaccaatat gaagtctttt cgttaatcgc tctcggttat
      421 ccgcaagatg ccgatgcttt tcgcttcgtt gatcgcttcg atcccacaaa gatttcctat
      481 gaagagttct aaagatgccg gaattaccgg aagttgagac ggtccgtcgg acccttgcga
      541 ccttagttgt cggcaaaacg attgccgatg tttcggctcc ttacccgaaa atcatcaaga
      601 atctatcttt ttcgcatttt cgggggacgc ttattaatga aaccattacc gacattaagc
      661 ggaaagggaa gtttttaatt gtggtgttta ccaaatgtgt cttggtttcg catttacgga
      721 tggaaggaaa atatttttat aaaacgactc ctgcgattga aaaacatgaa catgtcattt
      781 tttggtttac ggacggctcc agtttgcatt atcatgacac ccgcaaattt gggacgatgt
      841 atttgtttcc aacaagggat atgaccttaa ttactcagct tgaacccctt tctcaagttg
      901 gcttagagcc catggatgcg aacatgaatg tcgcttactt aaaagctcgt tttcaaaagt
      961 ctacgaaagc cattaaaaca acacttttgg accaaaagat aatggcggga ctggggaata
     1021 tttatgttga tgaagttttg tttttatcca aaattcatcc cgaaacgccg acaaaaactc
     1081 ttagtgatga gcaaatcgcc ttaatcatta ccaatgcgca aaccgttcta acaaaagcga
     1141 ttgctcttgg ggggacgact atccgcagtt ttacttcggc tcatgatgtt tctggtcgtt
     1201 ttcaaaacga acttttggtg cacactaaga cggaatgtcc ggtttgccaa gcgaaaatta
     1261 aaaaaattaa agttgggggt cggggcactt atgtctgtga aaattgtcaa aagcccttga
     1321 catagaaatt taaaaagcat acactaaata cacattcttg ggggagatta tgattgttgg
     1381 catcaccggt tccattgctt ccggtaaatc acttgtaact agctatttag cagaaaaagg
     1441 ctatcaaatc atcgattccg atgtgattgc gagagctcaa ttgcaaaaac cggaagtgat
     1501 tacgaagctt agggctgctt tcggaaaaga aatcattcaa gatgagcaaa ttaaccgaag
     1561 agttttagga aaaatcattt ttcaagatga acaaaaacgc caaattttaa atcaaatttt
     1621 acatccttta attatccaag aaattaagca cttaattaaa gatgccaagg aattggtgtt
     1681 tgtggatgtt ccgcttttgt atgaagccaa gatggaagca ctatttgata aaataattgt
     1741 cgtgtggatt aatcgcgacc aacaattaaa gcgactaatg aaacgagatc gaatctcgga
     1801 agcatatgca ctacttaaaa tcaacagtca ggatgattta gaaagcaaaa aagcgcgggc
     1861 cgattttgtg attgacaaca acggtcgccc gactcatacc caatttcaag ttgaacaaat
     1921 cttaaggaga ttaaaaaatg aaaattagag attatttttc caacgatttt gaaaccggcg
     1981 aagatcatta tctgccttct ttaaaaacgc gttattaccg ttgtcggatt gaagatgtaa
     2041 aagcagccat cttaagattt gttgaggagg aaaaaggcgt tgtgaaagcg attatggatg
     2101 aacatcatga aatctttttt gtgactccca actattcatc aacgattacc tacatcaaca
     2161 cgcgcattgg tgaagcagct attgatatca aaataactac ttatcgactt attccttggg
     2221 gggcgggcaa aaaaacgatt gaaagaatgt ataaatattt tgatgcccac ttgccgttta
     2281 agggagtcag tctttatcga ggttcatgat ggccatttcc attagcgata attttaaaat
     2341 cacacaaaaa catgctttaa gcggggaaga ttattttgtg ctttcacaaa tgtatttacc
     2401 gattattggc attgacagct tttctctcta tcatttattg tttacgctta atgaaagtga
     2461 tacttacagt attaaaaagt tagtggactt tctttcctgc agttcgcctt cttttttaga
     2521 acaagctctc cataaactgg aaggtgtgtc cttgattgat acctattatc atgagcacaa
     2581 gggttattta tttaatttaa aaacaccctt atcccgaacc tcttttttag aaaattcttt
     2641 attggcttca tttttatcca tgcaaattgg tgaagttgaa atgaagaaaa tgcaagaaca
     2701 agacaaaatc aatgttcgcc aatataaaga aatttcaaaa gcttttgatg aagtctatga
     2761 tgtttccacg gataatgtca gcaatttgtt taagcgctta tttaaagtta agaaaaacaa
     2821 aggtattaaa gtcatcaacc cggattttga ttatattgtt tttaaaatga gttttgatac
     2881 ggatttcttt gaccaaaaat tattggacga tgaagacttt aaacaacata tcttatccat
     2941 cagttataat tacaaactaa atgaagaaga gatgaaagaa gtcatcgaaa agacgatttc
     3001 cgcggaccgc gatctcaaat ataccgacat ttccaagcat gctaaagcga tgtttcaatt
     3061 aaaacacaaa caacaaaggc cacgcatttt aactaaaact cccgatgcct tcatgcaaag
     3121 ccaaatggat gatgattatt atcgggtttt tgaagtatta gagaataaaa ctccgagcga
     3181 tattttagcg gaattatccg gcattcaacc ctcggttgcg gaagtggaaa tgttccaaca
     3241 attgattgac aacacgaagt ttccgattag cgttatcaat ctaatgattt tatatgtcaa
     3301 tactttaaaa aagggcgagt taccgaatta taattatttt gaaaaattgg cgaatgtgtg
     3361 ggcacgaatg ggcattaaaa atgtgaaaga tgctttggat tatctcaatc aacccaaacc
     3421 aaccaaggaa gtaaaaaagg ggatgtatgc gaaaaaagaa aaacccttgc cggattggta
     3481 tgatcaatat accacgcaac tgagcaagaa gaaggaaaac gaaacgcttt cggaagagga
     3541 agtcaacaaa attctctcag aagcagaaaa gaaatataac taggagctgt tatgagcaat
     3601 gaatttaaaa aaatcaccaa ggctattttt gaagataaac tcttaaagga gtttgtagaa
     3661 aaaaacaagc ttagtgaaga agacatttta aataatctta gtcgttttta tattcaaaaa
     3721 gagaataatg ctatttgtct tccatgtgat gggagcaact gtcaaatgga tcccaaagcc
     3781 atgcaaacaa gattggtgat ggaaggcaat ttggatttag tttatttccc ttgtccgaag
     3841 tacgaacaag ccatcagcga taatttggaa atgctctttt tttcacaacc cgctcttttt
     3901 gaaaaccaga aattatttat taataatgaa cgggtctttg cccttaagca tatggatatg
     3961 tttttaaaga aatatcaaaa aggggtcttt accaagggac tttacctcca tggtgctttt
     4021 gggacgggaa aaactttcat tctttggcag ttagctaaac aattaacgaa aaaaggtgtt
     4081 aaggttatga tggtttacta ccccgattta gtccgtacca ttaaaagcta tatcgctact
     4141 tcggagtttg aaccgctcat taaccgttta aaatatattg atgtgcttat tttagatgat
     4201 gtcggagcgg aaaataatac ggctttcatt cgtgatgaag tattgggtcc cattttacaa
     4261 ttccgtcgtg atgctctttt accggtgtgt atgagttcca actacaattt tacattgttg
     4321 cgtgaccatt tttccgaaac caaggatgaa attaaccata ttaaaagcgg tcggattttg
     4381 gagcgcattc agtcattaat ggattctatt gaattggttg ataagaatta tcgcgctgtt
     4441 gattctaatt cgaagtaaag ttattaagaa g
//
LOCUS       DAOSGN010000019         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_17646_length_4454_cov_2.922255, whole genome shotgun sequence.
ACCESSION   DAOSGN010000019 DAOSGN010000000
VERSION     DAOSGN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17646_length_4454_cov_2.922255"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PK087_00625"
     CDS             complement(<1..286)
                     /locus_tag="PK087_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17802.1"
                     /translation="MEFNEKTYLCKDVYTIDGVLNFDGKIPIDINGRTISFKMDAHFS
                     DLLDSECFEVKNPGLKLYVHYDESLKADAYYLNIVTDNKIIIKAVNMRGIR"
     gene            complement(289..1254)
                     /locus_tag="PK087_00630"
     CDS             complement(289..1254)
                     /locus_tag="PK087_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17803.1"
                     /translation="MSLRIQVVPTYDSRAFDVRPFLNPKVYRGPIVNEKAPMTSPPCF
                     LGAWYRKVFSSFDAWLGIEGVIELGEFTPDEARFNLDGQGRYMDVPSIYMGGKSHFES
                     DAGLGLGPTYLSNDFSMELNQSSPKLAYRPFWRYIYQDASELNGNVKRREINSWNVTN
                     PRSVIYNYFPGDVLRMSVYSPIIDYLQLHIELIKTTTIPQYMNIRAQYHLENNRPDDF
                     FSPLFFSKGHGIDDAEFKRVNAIDQFGNEGFHAQATKAKVSKAVWHETYLYRLLDGEI
                     RKVPFSKARQTRMICPNNEAITVEALNEAIGSEAITIHPGEVKGG"
     gene            complement(1256..1957)
                     /locus_tag="PK087_00635"
     CDS             complement(1256..1957)
                     /locus_tag="PK087_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17804.1"
                     /translation="MSRILTSLKTKPMTLVVSLPENTYEMAKAAWEAGADAIKVHINL
                     FHNASLVKFGTLAEQRPILEAILRDCPVPVGVVAGQDTKTSEAVIEELVEMGFDFISL
                     YGHHMPVSLTGRSDVVNLFAIDYSYSLEEVKTITNSFIADILELSILPHEEYGLRLNA
                     RDLGYYLTFSQVSSIPTVLPSQRLLYPSDVKAIAKCGVKGIMIGAIVMGHDIDKMVSQ
                     IKQFKKAIAELPKKE"
     gene            complement(1932..2354)
                     /locus_tag="PK087_00640"
     CDS             complement(1932..2354)
                     /locus_tag="PK087_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17805.1"
                     /translation="MKDQLKIFLLRSWVIGMVVVVVHFMMGFQHLFIGLILGIINTFF
                     VDLVILTITKGNQAHFSTGLKLFYRTVFNIAVAIIISLLIRLIDLQLLKKNIITMPIE
                     TFRFIAYYQIIYYGSFYLYKKIYNLIERKKNESHSNKS"
     gene            complement(2354..3220)
                     /locus_tag="PK087_00645"
     CDS             complement(2354..3220)
                     /locus_tag="PK087_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH17806.1"
                     /translation="MLESKSDKIFSLVVYIILSIFGLSIIMPFLNLLATSLSDQVAVS
                     NGSVSFWPKNFTWSAYQFVIQNNQFWNGLKTTVFVTLVGTTLALMVTTLAAYPLSLPK
                     FKARKFFLVIFIIVMLFNGGLIPTYLLINQLGLRNNRWALILPSLISTYNMLLVKNYF
                     ETLPEALYESAKIDGATEFQIFIKITLPLSLPTLATVALFYAVGFWNAYVNGMLYIST
                     PSKMPLQTYLQTLLNMAYIPAHELSPEVAESLSTESIRAATIFSATIPILVLYPFLQK
                     YFVKGLVLGSDK"
     gene            complement(3222..4160)
                     /locus_tag="PK087_00650"
     CDS             complement(3222..4160)
                     /locus_tag="PK087_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH17807.1"
                     /translation="MKKKPNKLLKHFKQYGALYFFLIPGLILVAIFNYAPMAGIVIAF
                     KNYDIFAGRTPVESIIKSEWVGFAHFKTLFSNSDFTRAFRNTLIISTYKIVFLFPIPI
                     ILAIMITDIKALWYKKVIQTILYLPHFLSWIVVTALFLQILGPYGVINNWLKNLNIIE
                     QSIAFFGNNDIFRGLVVISAGWKEVGWNAIIYIAAITGINPQLYEAAKIDGASKMQQV
                     IYITIPSIANTIIMLFILRIGAILDAGFDQIFNMYSYVVYDKGDILSTLVYRSGIGDQ
                     NYSFATAVGLFNSVVALVMVLGGNYLSRKIFKKGLW"
     gene            complement(4144..>4454)
                     /locus_tag="PK087_00655"
     CDS             complement(4144..>4454)
                     /locus_tag="PK087_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17808.1"
                     /translation="IMIIETSLMAIMVFFLNGLFVFQKFSDLLKMSFYILHRHVLTTI
                     IMTLSVVILVYVFVIYLNGLFLLLFFSLICLWINLLYWPIVNKYIVKKEEELEHEKET
                     "
BASE COUNT         1531 a          888 c          804 g         1231 t
ORIGIN      
        1 agcggatacc gcgcatatta acggctttaa taataatttt attatcggtc acaatgttta
       61 aataataggc gtcggctttt aaactttcat cataatgaac atatagcttt aagccggggt
      121 ttttaacttc aaagcactca ctatcaagta aatcggaaaa atgagcatcc attttaaaac
      181 ttatcgtccg cccattgata tcaataggga ttttcccatc aaaatttaaa acaccgtcaa
      241 tggtgtaaac atccttacac aaataggttt tttcattaaa ctccattttt accctccctt
      301 aacctcccct ggatgaatag taatcgcttc actgccaatc gcttcattaa gagcttcaac
      361 agtaatagcc tcattattag gacaaatcat cctcgtttga cgtgctttcg aaaacgggac
      421 ttttctaatt tctccgtcaa gcaagcgata taaataagtt tcatgccaaa ccgcttttga
      481 caccttagct ttggttgctt gcgcatggaa accctcattc ccaaattggt caatggcatt
      541 gacccgtttg aattccgcat cgtcgatccc gtgaccctta gagaaaaaca aaggactgaa
      601 aaaatcatcg gggcggttat tttctaaatg gtattgcgct ctgatgttca tatattgggg
      661 aatagttgtc gttttgataa gctcaatatg taactgtaaa taatcaataa tcggactata
      721 aacactcatt cttaaaacat caccggggaa ataattatag ataacacttc tgggattagt
      781 gacattccaa gaattgattt cccgacgctt gacatttccg tttagctcgg aagcatcttg
      841 gtatatataa cgccaaaagg gtcgataagc cagtttcggg ctcgactggt ttaattccat
      901 actaaaatca ttgcttagat aagtggggcc aaggcccaaa ccggcatcgc tttcaaagtg
      961 gcttttacca cccatgtaaa tactgggaac atccatataa cgaccttggc cgtcaagatt
     1021 aaagcgtgct tcatcgggcg taaattcacc cagttcaatc acgccttcaa ttcccaacca
     1081 agcatcaaac gaagaaaaaa ctttccgata ccaagcccct aaaaaacaag gcggcgaagt
     1141 cattggggcc ttttcattta caatcgggcc ccgataaact ttgggattca gaaaaggacg
     1201 aacatcaaag gcccggctgt cataagtcgg aacaacttga atccttagtg acatttcatt
     1261 ccttttttgg caactcggca atcgcttttt taaattgttt gatttgcgaa accattttat
     1321 cgatgtcatg acccataaca atggcaccga tcataatccc tttcaccccg catttcgcaa
     1381 tcgctttgac atcgctcgga tacagtaacc tttgggaagg caaaacggtt ggaatgctgg
     1441 aaacttggga aaaagttaag taatacccca aatcacgggc attaaggcgt aagccatatt
     1501 cttcatgcgg aagaatggat aattctaaaa tatccgcaat aaaggaattg gtaatggttt
     1561 taacttcttc tagactgtaa ctatagtcaa tagcaaatag attgacgaca tcggaacgac
     1621 cggttaatga caccggcatg tgatgaccgt ataaggagat aaaatcaaaa cccatttcaa
     1681 ctaattcttc gataaccgct tcgctagttt tcgtgtcctg cccggcaaca acgccgaccg
     1741 gaaccggaca atcgcgaagg atggcttcta aaatcgggcg ttgttcggcc aaagtgccaa
     1801 atttaactaa actggcatta tgaaaaaggt tgatgtgaac tttaatggca tcggcgccgg
     1861 cttcccaagc agctttagcc atttcataag tgttttccgg taaactgacg accaaggtca
     1921 taggcttggt cttaagactt gttagaatgc gactcatttt tcttcctttc aattaaatta
     1981 tagatttttt tataaagata aaaactaccg taataaatta tttgataata agcaataaac
     2041 cgaaaggttt caatcggcat cgttattata ttctttttca acaattgtaa atcgattaga
     2101 cgaatcaata aactgataat tatcgccacg gcaatgttga aaacagtgcg gtagaataac
     2161 tttaatcctg tggaaaaatg ggcttgatta cctttagtga tggttaaaat gacaagatcc
     2221 acaaaaaagg tattaattat tccgagaatg agaccaatga acaaatgttg aaatcccatc
     2281 ataaaatgaa caacgacaac caccataccg ataacccatg atcttaaaag aaatatcttt
     2341 aattgatctt tcattatttg tccgagccca aaactaagcc tttcacaaaa tatttctgta
     2401 agaacgggta gagcacaaga atgggaatcg tggccgagaa aatcgtggcg gcgcggatgg
     2461 attccgttga caaactttcc gcaacttccg gcgacaattc atgggccgga atataggcca
     2521 tattgagcaa agtttgtaaa tatgtttgca agggcatttt gctcggagta gaaatataga
     2581 gcatcccgtt aacataggca ttccaaaagc caaccgcata gaataaggcg acggtcgcca
     2641 aggttggtaa ggataagggc aaagtgattt taatgaaaat ctgaaattcg gtagcaccgt
     2701 caattttagc cgactcatat aaagcttccg gcaatgtttc aaaatagttt ttgaccaata
     2761 gcatattata agtcgaaatc agcgacggca aaattaaagc ccagcggtta ttacgtaaac
     2821 caagctgatt aatcaacagg tatgtcggaa tcagcccccc attaaacaac atgacaatga
     2881 taaagataac taagaaaaat tttcgtgctt taaacttcgg taaacttaag ggataagccg
     2941 ccaaggttgt taccattaaa gctaaagtgg ttccaacaag agttacaaac accgttgttt
     3001 ttaaaccgtt ccaaaattga ttattttgaa taacaaattg ataggcactc caagtaaagt
     3061 ttttcggcca aaacgaaacg gagccattag aaaccgctac ttgatcggaa agggaggttg
     3121 ccaaaaggtt taaaaagggc ataataatcg ataatccgaa aatcgaaaga atgatataaa
     3181 caactaaact aaatatttta tcgctttttg attctaacat cttaccacag tcccttctta
     3241 aaaattttcc gtgatagata attgccacca agaaccataa ctaaggcgac caccgagtta
     3301 aacagcccga cagcggttgc aaaactgtag ttttggtccc caatccctga acgataaact
     3361 aatgttgata aaatatcacc tttatcataa acaacataag aatacatatt gaaaatttga
     3421 tcaaaaccgg catcaagaat ggcaccgatt cttaaaataa acagcataat aatcgtattg
     3481 gcaattgacg gaatggtgat gtaaatcact tgttgcattt tgctggctcc gtcaatttta
     3541 gcggcttcat acagttgcgg attaatgccg gtgatagcgg caatatatat aattgcattc
     3601 caaccgactt ccttccagcc cgccgatatc accactaaac cgcggaatat atcattgttg
     3661 ccgaaaaaag cgattgactg ctcaataata ttcaagtttt taagccagtt attaatgacc
     3721 ccataaggtc ctaaaatctg aagaaaaagg gccgtaacca caatccaaga caaaaagtgt
     3781 ggtaaatata agattgtttg gatgactttt ttataccata aagccttaat gtcggtaatc
     3841 ataatggcta gaataatggg aatcggaaat aaaaagacaa ttttataagt actgataatg
     3901 agcgtgtttc gaaaggctct cgtaaagtca ctattgctaa aaagggtttt aaaatgagca
     3961 aagccgaccc attcactttt aatgatggat tcaacgggcg tgcgtccggc aaaaatatca
     4021 tagtttttaa aagcaatcac aatgccggcc atgggagcat aattaaagat ggccaccaaa
     4081 attaaaccag gaattaagaa aaaatataaa gcaccatact gtttaaaatg ctttaacagt
     4141 ttgttaggtt tctttttcat gctctagttc ctcctccttc ttgacaatat atttattgac
     4201 aatcggccaa tataacaaat tgatccataa acaaattaaa ctgaaaaaca ataataaaaa
     4261 caaaccgtta agataaatca caaagacata aactaagatt acaacgctca aagtcatgat
     4321 aatggtggta agaacatgac gatgcaaaat ataaaaagac attttcaata agtcagaaaa
     4381 cttttgaaaa acaaataagc cgtttaaaaa aaagaccatg attgccatta gtgaagtttc
     4441 aataatcata atat
//
LOCUS       DAOSGN010000020         4422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_17826_length_4422_cov_3.916648, whole genome shotgun sequence.
ACCESSION   DAOSGN010000020 DAOSGN010000000
VERSION     DAOSGN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17826_length_4422_cov_3.916648"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PK087_00660"
     CDS             complement(<1..671)
                     /locus_tag="PK087_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOH17809.1"
                     /translation="MKPWRDLTTTNPHIANIVLASVALDRNASVGKGASLAPRKLRQL
                     SRYLPPASKDGTDLTAIKILDLGSIGNNFSAFSPIEQRAKTIFDLNKFSVFLGGDHSV
                     AIPLQGAFLEYCQEQDKIPAIIHLDAHPDICDTYDGSKYSHACPNFRAMERGYQRENI
                     KMLGIRGFELQEIELFKQYPDLEVVTASVLNVRGLDAIKHFKDKFDERYLIYLSFDID
                     IIDPS"
     gene            complement(970..1752)
                     /locus_tag="PK087_00665"
     CDS             complement(970..1752)
                     /locus_tag="PK087_00665"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOH17810.1"
                     /translation="MKKPIIISILVVLGVFGLSGCGLFWPKEYDPENNKNSTPIQTDF
                     FKVQLFEVDYRGYKNKWARVYGLTELGKEQEILAIPEYIEGFPVTLIGVLGSKKYGIK
                     SDNLKKIYIPQTIYSRMYRGLPIAAQSISCQDAVVFLTAEEINLSGIVRKDTMIIMMP
                     DAFTRSTQGLLEDFVANIKYCSPNIIYHYNYETSPNQDIYWLDFIEKDSLYLNPESPK
                     REGYIFTGWYVDINCQEKWDGTYPSNGEDIVEIYAGWEEEPS"
     gene            complement(1842..2624)
                     /locus_tag="PK087_00670"
     CDS             complement(1842..2624)
                     /locus_tag="PK087_00670"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOH17811.1"
                     /translation="MKKSIIISILVVLGVFGLSGCGSKPEEYDPENRKNSTSIQTDFF
                     KVQLFNVDCREYYDKWARVYGLTELGKKQEILVIPEDIEGFPATMIGVLGSKKYGIKS
                     DNLKKIYIPQTIYSRMYRGLPIAAQSISCQDAVVFLTAEEINLSGIVRKDTMIIMMPD
                     AFTRSTQGLLEDFVANIKYCSPNIIYHYNYETSPNQDIYWLDFIEKDSLYLNPESPKR
                     EGYIFTGWYVDINCQEKWDGTYPSNGEDIVEIYAGWEEEPSL"
     gene            complement(2886..>4422)
                     /locus_tag="PK087_00675"
     CDS             complement(2886..>4422)
                     /locus_tag="PK087_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH17812.1"
                     /translation="IFGTYAQRLSTNRVVSLIARDARTDAMRSIERLPIKYFEAEPAG
                     KMAARITHDVDGMIGLYRLSVNVMAYAVLSFLFAYVGMFILDVKLALLTFIAYPFIYL
                     WVRYFLKHLRRIAEKVNELRSLMTAKINEIINGINILQIFNFKKPTIKEFNQINKDFT
                     NEQMQEVKLHLTAGWNLINIMRGLVTTFIVAYFGWQKLNISGVVITAGLVYAYNEYLL
                     KIIEPVNIIFTQVGEYQHSMVRIERICKLIEGELEDNTIGPIDRYNGNIKFDNIWFAY
                     VENEYVLKGVSLEIKHGERVGLVGHTGSGKSSLMNLLLRFYDLTDEKSGKIYVDGIDI
                     ASLPKRTYRYHLGIVLQEPILFKGTIASNIRFGKENVSDEQIIAILKSMGGEKLINKF
                     EKGINTPISRAGVNMSSGEKQIISLARVIVHNPSILVMDEATSHIDTETEEMIKKALK
                     VVCKNRTVIIIAHRLSTIYNADRIIVLDHGLKVEEGTHHTLMQANGVYANIYRAQVAN
                     SDF"
BASE COUNT         1353 a          905 c          718 g         1446 t
ORIGIN      
        1 aatgatggat cgatgatgtc aatatcgaag gaaagataaa ttaaataacg ctcatcaaac
       61 ttatctttaa aatgcttaat ggcatctaaa ccacggacat ttaagacgga agcggtcaca
      121 acttctaagt cgggatattg tttaaacaat tcgatttcct gcaattcaaa gcctctaatg
      181 cccagcattt ttatgttttc ccgctgatag ccccgttcca tcgcccgaaa gttggggcaa
      241 gcatgggaat atttacttcc atcataggta tcacaaatat cggggtgagc atcaaggtga
      301 atgatggcgg ggattttatc ttgttcttgg caatactcta agaaagcacc ctgcaaaggg
      361 atggcaacag aatgatcacc accaagaaaa acggaaaact tattgaggtc aaaaatagtc
      421 ttggctcttt gttcgattgg ggaaaaggcc gagaaattat ttccgatgct acctaaatcc
      481 aagattttga tggctgttaa atcagtgccg tctttggatg ccggtggcaa ataccttgat
      541 aattggcgca gttttcgggg tgctaagctc gcccctttgc caaccgaagc attacggtca
      601 agggctacgg aagctaaaac aatattagcg atatgagggt tggttgttgt taaatcacgc
      661 catggtttca ttactctttc tcaccttctt tcatgtcctt aacggcttta attataacat
      721 ttattttaaa tatagcaatt atttttatta ttattaagct ggctattaaa aagttcacaa
      781 acaaaatgtt tgcgaactta tgttaaaaat cagaattagc cacttgcgct cggtaaatat
      841 tggcataaac accattggct tggttgtgaa aaacattaac aagcaagctg ctataacaaa
      901 gatgcctata acttgtaaag attttactat gttaggtgaa gtttaaattt aattatcctt
      961 tctcgtttgt taacttggtt cttcttccca acccgcatat atttccacaa tatcttcgcc
     1021 attagaaggg tatgttccat cccacttttc ttggcaatta atatccacat accaaccggt
     1081 aaatatatac ccctctcttt taggagattc cggattaagg tataaactat ctttctcaat
     1141 aaaatcaagc caataaatgt cttggttggg cgatgtttca taattgtaat gatagattat
     1201 attaggtgaa cagtacttaa tgttggcaac aaaatcttcc aaaagacctt gtgtagacct
     1261 tgtaaaggca tcgggcatca taataatcat agtatccttt cttacaatac cactaagatt
     1321 tatttcttca gctgttaaaa aaacgactgc gtcttggcat gaaatcgatt gtgcagctat
     1381 tggcaacccc ctatacatcc ttgaatatat cgtctgtgga atgtaaatct tttttaaatt
     1441 atcgctctta attccatatt ttttagatcc gagcacacca atcaaagtta ctggaaatcc
     1501 ttcaatatac tcaggaatgg ccaatatttc ttgttccttc ccaagttctg ttaaaccata
     1561 tactctggcc catttattct tatatcccct ataatctact tcaaataact gcactttgaa
     1621 gaaatcagtt tgaattggag tagagttttt gttgttttca gggtcatatt ctttaggcca
     1681 gaagagtccg catccactca agccaaagac tcccaacaca accagaatgc ttatgattat
     1741 cggttttttc atagtttcaa aacctccttg atgacggcag ataatgccca actaaaacca
     1801 ttctagtgtt ttcattattt gtcaactcat tatattagtt gttataaact tggttcttct
     1861 tcccaacccg catatatttc cacaatatct tcgccattag aagggtatgt tccatcccac
     1921 ttttcttggc aattaatatc cacataccaa ccggtaaata tatacccctc tcttttagga
     1981 gattccggat taaggtataa actatctttc tcaataaaat caagccaata aatgtcttgg
     2041 ttgggcgatg tttcataatt gtaatgatag attatattag gtgaacagta cttaatgttg
     2101 gcaacaaaat cttccaaaag accttgtgta gaccttgtaa aggcatcggg catcataata
     2161 atcatagtat cctttcttac aataccacta agatttattt cttcagctgt taaaaaaacg
     2221 actgcgtctt ggcatgaaat cgattgtgca gctattggca accccctata catccttgaa
     2281 tatatcgtct gtggaatgta aatctttttt aaattatcgc tcttaattcc atatttttta
     2341 gatccgagca caccaatcat agttgctgga aatccttcaa tatcctcagg aatcaccaat
     2401 atttcttgtt tcttcccaag ttctgttaaa ccatatactc tagcccattt atcataatat
     2461 tccctacaat ctacattaaa taactgtact ttgaaaaaat cggtttgaat tgaagtagag
     2521 tttttgcgat tttcagggtc atattcttca ggcttgctcc cacatccact caaaccaaag
     2581 actcccaaca caaccagaat gcttatgatt atcgattttt tcatagtttc aaacctcctt
     2641 gatgacggta aataatgccc aactaaaacc attctagtgt tttcattatt ttactattat
     2701 tatggtaata attaaaaaca tttcaaattg ttgttttcct taaaatcttg tcaattttat
     2761 tttattatga actacattat ttgtcaattt aattgaaatt gtttgctttc tccttattta
     2821 ttgcctttct ttttagtttt tgtggtcatt aaaaagttca caaacaaaat gtttgcgaac
     2881 ttatgttaaa aatcagaatt agccacttgc gctcggtaaa tattggcata aacaccattg
     2941 gcttgcatta aggtatgatg tgttccttct tccactttta aaccgtggtc taaaacaata
     3001 atacggtccg cattgtaaat cgtacttaaa cgatgcgcaa tgatgattac ggttcggttc
     3061 ttacaaacca cttttaaagc ttttttaatc atttcctcag tttccgtatc aatgtgggaa
     3121 gtcgcttcat ccataaccaa gatggaagga ttatggacaa taacccgagc caaactgatg
     3181 atttgttttt caccgctgct catgttaaca cccgcacggc taatcggtgt attaatgcct
     3241 ttttcaaatt tgttaatgag tttttcgcca cccatggatt ttaaaatcgc aataatttgt
     3301 tcatcgctca cattttcttt tccaaaacgg atattggagg caattgtccc cttaaacaaa
     3361 atcggttctt gtaaaacgat gcctaaatga taacgataag tccgtttcgg aagactggcg
     3421 atatcaatgc cgtcaacata aatctttccg gatttctcat cagtcaaatc ataaaaacgc
     3481 agcaataagt tcattagtga gcttttaccg cttccggtat gtcccaccaa gccaactctt
     3541 tccccgtgtt tgatttccaa actgacacct tttaaaacat attcgttttc cacataggca
     3601 aaccaaatgt tatcaaattt aatattgccg ttataacgat caatcggacc gatagtgtta
     3661 tcttccaatt ccccttcaat caatttgcaa attcgttcaa tgcggaccat ggaatgttga
     3721 tattcaccga cttgtgtaaa gatgatattc accggttcaa taatcttcaa caaatattca
     3781 ttataagcat aaaccaagcc ggcggttatc actactccgg agatgttcag tttttgccaa
     3841 ccaaagtaag ctacgatgaa agttgtaact aagccccgca taatgttgat taagttccat
     3901 cccgctgtta aatgcagctt gacttcttgc atctgttcat tggtaaaatc tttattaatc
     3961 tgattgaatt ctttgatggt cggtttttta aagttaaaga tttgaagaat attaatgcca
     4021 ttaataatct cattaatctt ggccgtcatt aagctgcgta attcattgac cttttccgca
     4081 atcctcctta aatgctttaa gaaataacga acccacaaat aaataaaggg ataagcaata
     4141 aaggtcaata aggctaattt cacatccaag atgaacatac cgacataagc aaagaggaaa
     4201 ctaagcaccg cataagccat gacattaacg cttaagcggt ataagccaat catcccgtca
     4261 acatcatgag taatacgggc cgccatctta cccgccggtt ccgcttcaaa atatttaatt
     4321 ggtaagcgct cgatactgcg catggcatcc gtccgggcat cacgagcaat caaactgaca
     4381 acacggtttg tggaaaggcg ttgggcgtag gtgccaaaga ta
//
LOCUS       DAOSGN010000021         4269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_18699_length_4269_cov_2.823683, whole genome shotgun sequence.
ACCESSION   DAOSGN010000021 DAOSGN010000000
VERSION     DAOSGN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18699_length_4269_cov_2.823683"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PK087_00680"
     CDS             <1..617
                     /locus_tag="PK087_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH17813.1"
                     /translation="GFSMLRLRSLPTMAVMAKRGNIMPHLHLPVQSGSNLVLKRMNRK
                     YTKEEYLEKIAKLRALVPGIAITTDIIVGFPGETDDDFNETLDLVRKAHFEGAYTFIF
                     SPRSGTPASSYEDELDPKIKKQRLLTLNALINDGFLKGNQRFEGQVVQVLVEGFSAKN
                     AKQLTGYTEHNKLINFEGPETLIGQIIPIKVEKAYTWHLQGKIV"
     gene            complement(641..1801)
                     /locus_tag="PK087_00685"
     CDS             complement(641..1801)
                     /locus_tag="PK087_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOH17814.1"
                     /translation="MSKVAIAKCQQYELEQVKTAIKNTLDHLGGLNQLIPQNAKVFIK
                     INGLGPFSPSMGITTHPVFVQAIIQLVKTRTANILIGDNPATKEIHHVMKKNGVYDVV
                     VQEQIPIFNGKTLTTIHNPSAKIYKTFEVSQEMLDTDVLINLPKLKTHSLTYVTIAQK
                     NLFGFIYGLSKGGWHVKASNPLQFGEALNDLYGAILHAFKGKTLLHLCDGIIGLEGDG
                     PSSAGHPKQAEVILASLDAVSLDRVAIELMHLNPEKAFVNTIAQERGYGEGHLKNITI
                     VGEPLSTFQDLHFVPPGNTVSQFGLKLLKIKPLRNFVLEHPKIDHSLCIKCGECVRIC
                     SSKAMTIKARKFPELKKNLCIRCWCCAEVCPQNAIKKSKRPLVGRIVLKDRM"
     gene            complement(1803..2162)
                     /locus_tag="PK087_00690"
     CDS             complement(1803..2162)
                     /locus_tag="PK087_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOH17815.1"
                     /translation="MRIYESGENYLEAILILKSSQPEVHAIDVANYMSFSKPSVSRAL
                     AILKKAGLITIDKSGVIELTATGHQKATKIYDRHLTITRFLVDLGVDDELAERDACKI
                     EHVISEETFQAIKKQVK"
     gene            2285..3061
                     /locus_tag="PK087_00695"
     CDS             2285..3061
                     /locus_tag="PK087_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH17816.1"
                     /translation="MKETVIVKNLTKTFQLSKKQMKINQTTEKVKVAVDDLSFSTFEG
                     EIFGLLGPNGAGKTTTLRCIATLIKPDKGEITVSGIDIKDEVSIKREIAFLTNELKLE
                     EQFTPNYLYDYFSKLYDVEEATMLARKQLLFDRFGVSKFAEVKIGELSSGMKQKVSIV
                     ISLVHDPKIVIFDEPTNGLDVITAKTVTDYLLELKEQGKTVIISTHIMNLVEKICDRA
                     GIIIDGKMRLCDRVDALLKNHPGQDIEEIFFDIYSEVSHE"
     gene            3054..4229
                     /locus_tag="PK087_00700"
     CDS             3054..4229
                     /locus_tag="PK087_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH17817.1"
                     /translation="MNDVFKIFKKEMDKIFRFPRMIFTSLVLPGLIIFLIYAFIGQSM
                     QAEMERAEEYRGLVSIINAPDSLAFAINNPDNFHLSFEEKPSSEQDNLIELVKSGELD
                     VLVVYQTGFDADVAAGQDPQIAVYYDSSKTNSNVTYAKIQMIIGIQRNNFLESLNIDP
                     NIFVVQDHIVTEENKLAAQILSMVLPMLIISFIFAGALSIGSDAIAGEKERGTLATLL
                     MAPLSRTNVIFGKILSTAVLTVFSAFSSFVGIIASLPFAKAMFPIEGGLSYSLWDYLG
                     ILLILIVLSVLASSLLLIFSTLAKTVKEATMLAMPLYLAAIIMPVLTMFSESVSKKFW
                     HYLIPIYNCTVGLKAVLSMEITLLNYLLIIASSLVFIVLTVFLLVKLFKSEKVLFAK"
BASE COUNT         1259 a          791 c          845 g         1374 t
ORIGIN      
        1 tcggtttcag catgctgcgc ctgcgctcat tgccgacgat ggcggtgatg gccaagcggg
       61 ggaacatcat gcctcattta catttacctg ttcagtcagg cagtaattta gttttgaagc
      121 ggatgaaccg taagtatacc aaggaagaat atttggaaaa gattgcgaaa ctgcgcgctt
      181 tagttccggg tatagcgatt acgacagata tcattgtcgg ctttcccggt gaaacggatg
      241 atgattttaa tgaaaccttg gatttagtga ggaaagccca ttttgaaggt gcctatacct
      301 tcatcttctc accaagaagt gggactccgg ctagtagcta tgaagacgaa ctggacccga
      361 aaattaaaaa gcaacgcttg cttactttaa atgccttaat caatgacggt tttttaaaag
      421 gcaatcaacg ctttgaaggt caagtggttc aggtgttggt tgaaggtttc agtgcaaaaa
      481 acgctaaaca attaaccggt tataccgaac acaataaatt aattaatttt gaaggtcctg
      541 aaactttaat tgggcagatt attccaataa aagtcgaaaa agcttatact tggcatttac
      601 aggggaaaat agtgtaaaaa aaagagcttc agctcttttt ttacattcgg tcttttaaaa
      661 cgatgcgtcc gactaacgga cgtttgcttt ttttaatcgc attttgcgga caaacttccg
      721 cacagcacca acaacggatg caaagattct ttttaagttc ggggaatttt ctcgctttga
      781 tagtcatcgc tttcgacgaa caaattcgta cacattcgcc acatttaatg caaagagagt
      841 gatcgatttt gggatgttct aagacaaagt ttcttaaagg tttaattttt agtagtttca
      901 aaccaaactg actaacggtg tttcccggtg gaacaaagtg taaatcttgg aatgttgata
      961 agggttcacc aacgatggtg atgttcttaa gatgcccttc accgtaacct cgctcttgag
     1021 caatagtatt aacaaaagct ttttcggggt ttaaatgcat aagttcaatg gccacacgat
     1081 ccaaactgac agcatcaagc gaagctagga taacttccgc ttgtttcggg tgtcctgctg
     1141 aagacggtcc gtccccttct aaaccaatga tgccatcaca aagatggagg agggttttcc
     1201 ctttgaaagc atgcaagatg gccccatata aatcatttaa agcctcccca aattggagtg
     1261 gattgctcgc tttaacgtgc catccgcctt tacttaagcc gtaaataaac ccaaataaat
     1321 ttttttgggc aatcgtaaca taagttaaac tgtgggtttt caatttgggc aggttaatta
     1381 acacatcggt atctagcatt tcctgcgaca cttcaaatgt tttgtaaatt ttcgcacttg
     1441 ggttgtggat ggttgttaat gtttttccat taaaaatggg aatttgttct tggacaacaa
     1501 catcgtaaac accgtttttc ttcataacat gatgaatttc ttttgtggcg gggttatcac
     1561 cgataaggat attagcggtt ctggttttta caagctgaat gatggcttgt acaaaaaccg
     1621 gatgggtggt aatccccatg ctcggagaaa aaggtcctaa accattaatc ttaataaaaa
     1681 ctttggcatt ttggggaatg agttgattca atccccccaa atggtccaaa gtatttttaa
     1741 tggctgtttt aacttgttct agttcatatt gttgacattt cgcaatcgca acttttgaca
     1801 ttttatttta cctgcttctt aatggcttgg aaagtttctt cactgataac atgttcaatc
     1861 ttacaggcat cgcgttccgc aagttcatca tcgacgccta agtcaactaa aaatctagta
     1921 atggttaagt ggcgatcata aatttttgtg gccttttgat gtccggtcgc agttaattca
     1981 atcactccgc ttttatcaat tgtaattaag ccggcttttt ttaaaatggc caaggctcga
     2041 ctaacgcttg gtttagaaaa ggacatataa ttggccacat caatggcatg gacttccggc
     2101 tgggatgatt ttaaaatgag tatggcttct aaatagtttt cgcccgattc ataaatacgc
     2161 atacttacct cttgttggaa taatcataca atatattatc ttgattgtaa agaaatagta
     2221 aaaaaccttt accgaacatt gctttatgat ttttaatatt ataaaattaa caagaggtat
     2281 gaagatgaaa gaaacagtta ttgtcaagaa tttaaccaaa acttttcaat tatcaaagaa
     2341 acagatgaag attaaccaaa ccaccgaaaa agttaaagtg gcggtagatg atttatcttt
     2401 ttccacattt gaaggtgaaa tctttggttt attaggccct aatggcgctg gaaaaacaac
     2461 cacccttcga tgcattgcga ccctaatcaa accggataaa ggcgaaatca ccgtttccgg
     2521 aattgatatt aaggatgaag tgagcatcaa acgagaaatt gcctttttaa ccaacgaact
     2581 aaaactggaa gaacagttta cacccaacta tttatatgat tatttttcaa aattatatga
     2641 tgttgaagaa gcaacgatgt tggcaagaaa acaattattg ttcgaccgtt ttggtgtgtc
     2701 taagtttgcg gaagttaaaa tcggcgaact ttcatccggg atgaaacaga aagtatcgat
     2761 tgttatttcg cttgtccacg atcccaaaat tgttatcttt gatgaaccga ctaacggttt
     2821 agatgttatt accgctaaaa ccgtaacgga ttacttatta gaactgaaag aacaaggaaa
     2881 aacagtgatt attagtacgc atattatgaa cctagtcgaa aaaatatgcg accgtgccgg
     2941 catcattatt gatggaaaaa tgcgtctttg cgaccgtgta gatgccttat taaagaacca
     3001 ccccggccaa gatatcgaag aaatcttctt tgatatttac agtgaggtca gccatgaatg
     3061 atgtttttaa aatctttaaa aaagaaatgg ataaaatatt ccgttttccg cgaatgattt
     3121 tcacttcgct tgtgcttccg ggattgatta ttttcctaat ttatgctttc attggtcaaa
     3181 gcatgcaagc ggaaatggag cgtgctgaag aatatcgagg tttagtttct attatcaatg
     3241 ctcctgattc cttggctttt gccatcaaca atccggataa tttccatctt tcgtttgaag
     3301 aaaagccaag tagtgaacaa gataatttaa tcgaactggt aaaaagtggt gaactggatg
     3361 ttttagtagt ttatcaaacc ggctttgatg ccgatgttgc tgccggtcaa gatccccaaa
     3421 ttgccgttta ttatgattca tccaaaacca attccaatgt tacttatgcg aaaattcaaa
     3481 tgattatcgg gattcagcgc aacaactttt tagagtccct aaatattgat ccgaatatct
     3541 ttgttgttca agatcacatc gtcacggaag aaaataaact tgctgcacaa attctatcca
     3601 tggttttacc gatgttgatt atttcgttta tttttgcggg agcattatcc attggttccg
     3661 atgccattgc cggagaaaag gaaagaggaa ccctcgcaac gcttttaatg gccccgcttt
     3721 cccggactaa tgttattttc gggaaaattc tttccacggc cgtgttaact gttttttccg
     3781 ctttttcttc ctttgtgggg attattgcct ccttgccgtt tgcgaaagcg atgtttccca
     3841 ttgaaggcgg cttaagttat tcgctttggg attatttagg aatcctatta atcttaattg
     3901 tgctttcagt gttagcatca agcctgttat taatcttctc gacgcttgcc aagacggtta
     3961 aagaagcaac gatgttagcg atgcccttgt atttggctgc tattattatg cccgtcctca
     4021 ccatgtttag tgaatcggtt agcaaaaagt tttggcatta ccttattccg atttataatt
     4081 gtaccgttgg attaaaagcg gttttgtcca tggaaattac cttgcttaat tatttattga
     4141 ttatcgcctc atcattagtg ttcattgtct taacggtttt tctcttagtt aaactgttta
     4201 agagtgaaaa ggttttattc gcgaagtagt ttatttgaca ttattcctcc agtttgctat
     4261 aatttaacc
//
LOCUS       DAOSGN010000022         4094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_19821_length_4094_cov_3.635058, whole genome shotgun sequence.
ACCESSION   DAOSGN010000022 DAOSGN010000000
VERSION     DAOSGN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19821_length_4094_cov_3.635058"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PK087_00705"
     CDS             <1..349
                     /locus_tag="PK087_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOH17818.1"
                     /translation="IPQLELGEEVCSATRARQTAIMDYKNQIDLLIVVGDPNSNNTKM
                     LKNVGENKAHLPTLFLENIEGLNGLNLSNYQRIGITAGASTPDVIVEEIIAGLKQAQT
                     IFKSNLQLDDYLK"
     gene            complement(371..2752)
                     /gene="pheT"
                     /locus_tag="PK087_00710"
     CDS             complement(371..2752)
                     /gene="pheT"
                     /locus_tag="PK087_00710"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOH17819.1"
                     /translation="MRVKLSWLNELVNISDLKVEELKKIISLYSTEVESVEQVATGSN
                     LLVGHVLQCEPHPDSDHLHLCLVDIGTEKLSIVCGAPNVSKGQKVVVATIGCVLPGGL
                     VIKKAKIRGVESSGMICSLAELGVEKKYIPEEYQAGIYYFKHDVKVGSSALAALDFDD
                     VVFDLAITPNRGDLMSMLGVAIEVAAVLNRPLKPLAYQVENEEKDVKLTVAIESNACH
                     AYLAQAFKNVQIKPSPWWLIARLIAFGIRPINNVVDITNYILALFGQPLHAFDYQKLG
                     SKIVVRQAREGEVFTTLDGVKRDLLASDLVITDGEKPVALAGVMGGKETEIVDGTTDI
                     VLEVAVFDPMTIRKTSQRLALRSESSARFERGVDVNRSQEALAYASYLLKNLAKAKPS
                     SEIAMAGLTNKGATLIAISENDVSQLLGIKVPLEEMRNLFERLQFKVEKGQDLLVKVP
                     NRRHDIKIKNDLIEEIGRLHGYTNLPNTLPKSAQAGALTPKQRRRRIIKQSLAALGLS
                     EAITYSLVNEQYNEAFADNHHPNSQAISLINPLSSDRGTLRKGILLSLIEVVKYNYAR
                     KMKDIALFEIGKGYSLNAVNQEEELLAIVMANRYFGNIWQKDVKVDFYVIKGVLENLA
                     KNLNINLTFKPMMESGQELHPRRSADVYLDNEKIGFIGSLHPQFALASGLDEVYVGEL
                     KLEKILQTSTSIKHYEPMSKYPHMERDLALVLPKEVLAGDIIESIKKTASLISDVYVF
                     DVYTGENVEKDQKSLAVRIIFTSHEPLSEEIISPKMNKIVKTLANEFKAQLRS"
     gene            complement(2762..3784)
                     /gene="pheS"
                     /locus_tag="PK087_00715"
     CDS             complement(2762..3784)
                     /gene="pheS"
                     /locus_tag="PK087_00715"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOH17820.1"
                     /translation="MDNQFKLLQERLLEELAKVKTMNELHETKALFLGKKSPIQELFA
                     RMKDLSIEEKKTLGQKVNWFKNYVEEAIAKKAEALEQAQIIAQLESEAIDVSLAGIAF
                     SLGNKHPLTKMTEEIEDIFIGMGYAIAEGPEVELDLYNFEMLNLPKGHPAREMQDSFY
                     IDGNTLLRTHTSPVQVRTLLAAKQQPVKIICPGKVYRRDYDDATHSYQFMQIEGLVVD
                     RGITMSDLKGTLTVLMQKMFGSSLEVRFRPSFFPFTEPSVEVDVTCFKCKGHGCSLCK
                     NGGWIEVLGAGMVHPQVLKMSGYDPNEFQGFAFGIGIERLTMLRYGIDDIRHFYTDDL
                     RFLKQF"
BASE COUNT         1236 a          919 c          703 g         1236 t
ORIGIN      
        1 cattccccaa ttagaacttg gggaagaagt atgcagtgcc accagagccc gccaaaccgc
       61 catcatggac tacaaaaacc aaatcgattt attgattgtg gtcggtgatc ctaacagcaa
      121 taacacgaaa atgctaaaaa atgtgggcga aaacaaagcc catttaccaa ccttgttttt
      181 agaaaacatt gaaggtttaa atggcttaaa tttatcaaac tatcaacgaa tcggtatcac
      241 cgccggtgct tcaacaccgg atgtgattgt ggaagaaatt attgccggtt taaaacaagc
      301 acaaaccatt tttaaatcaa acttacaatt agatgactac ctcaaataaa aagcggaaaa
      361 atccgctttt ttaacttcgt aattgggctt taaattcatt cgccaatgtt ttcacaatct
      421 tattcatttt aggactaata atctcttcac tcaatggttc atggctggta aagataattc
      481 gaactgctag agacttttgg tccttctcca cattctcacc tgtgtaaaca tcaaaaacat
      541 agacatcact aattagggaa gcggtttttt tgatgctttc aataatatcg ccggctaaaa
      601 cttctttcgg aagaacaagc gctaaatcgc gttccatatg gggatatttg ctcattggtt
      661 catagtgctt gatgcttgta gaagtctgta atattttttc cagttttaat tccccaacat
      721 aaacttcatc aagaccggat gctaaagcaa attgcggatg caaactgcca atgaaaccaa
      781 ttttttcgtt atctaaataa acatcggcac tacggcgtgg atgcaattct tgaccgcttt
      841 ccatcatcgg tttaaaagtt aaattgatgt ttaggttctt agctaaattt tccaaaacgc
      901 ctttgataac ataaaaatca actttaacat ctttttgcca gatattccca aaataccgat
      961 tagccataac aatggccaat aattcttctt cttgatttac ggcgtttaaa ctatagcctt
     1021 tcccgatttc aaacaaggcg atgtctttca tttttctcgc atagttatat tttacaacct
     1081 caatcaaact caacaatatc ccttttctta aggttccgcg atctgaactt aggggattga
     1141 ttagagagat ggcttggctg ttcggatgat ggttgtcagc aaaagcctca ttatattgct
     1201 cattaactaa ggaataagtg atggcttcgc ttaaacctaa agccgctaag ctttgtttaa
     1261 tgattcggcg tcgcctttgt ttaggcgtta aagctccggc ttgtgctgat tttggtaagg
     1321 tgttcggtaa attggtatag ccgtgaagac gaccgatttc ctcaattaaa tcatttttaa
     1381 tcttgatatc atggcgacga ttcggtactt ttaccaataa atcttgccct ttttccactt
     1441 taaactgcaa gcgttcaaaa agattgcgca tttcttctaa gggcactttg ataccaagta
     1501 attggctgac atcgttttcg gaaatggcaa tcaaagtcgc gcctttgtta gtaagtcctg
     1561 ccatagcaat ttcgctgctc ggcttagcct tagccaaatt cttaagtaaa taactggcat
     1621 aagctagggc ttcttgactg cggttaacat ccactccacg ttcaaaacgg gccgaggatt
     1681 cacttcttaa agctaagcgc tgggaagttt tgcgaatcgt catcggatca aaaaccgcaa
     1741 cttccaggac aatgtccgtg gtcccatcga caatctccgt ctctttacca cccattactc
     1801 ccgctaaagc gaccggtttt tcgccatcgg taattactaa atcactagcc agcaagtctc
     1861 ttttaacacc atccagggtt gtgaaaactt ctccttcacg agcttggcga accacaattt
     1921 tggaacctaa tttttggtaa tcaaaagcat ggagcggctg accaaacagc gccaaaatgt
     1981 aatttgtgat gtcaacgaca ttatttatcg gacgaatgcc aaaagcgatt aaacgggcaa
     2041 ttaaccacca aggtgaaggt ttaatttgca catttttaaa agcctgcgct aaataagcat
     2101 gacaagcgtt cgactcaatc gccaccgtta atttaacatc tttttcctca ttttctactt
     2161 gatatgctaa gggttttaag ggacggttta agacggcggc aacttcaatg gccaccccca
     2221 acatgctcat taaatccccg cgattagggg taatggctag atcaaaaaca acatcatcga
     2281 agtcaagtgc agctaaagcg gaactgccaa ctttaacatc atgtttaaaa tagtagattc
     2341 cggcttgata ttcttcagga atatacttct tttcaacccc taattccgct aatgaacaaa
     2401 tcattcccga tgattccacc ccgcggattt tagctttttt gataactaat ccgccaggaa
     2461 gaacacaacc gatggtcgca accactactt tttgtccttt tgaaacatta ggcgctccac
     2521 agacaattga caatttctct gttccgatat caactaaaca aagatgtaaa tgatcggaat
     2581 ccggatgtgg ctcgcattgt aaaacatgac caacaagcaa attcgacccc gttgccactt
     2641 gctcaacact ttcaacttcc gtactgtaaa gggaaataat cttttttaac tcctctacct
     2701 ttaaatcgga aatattaaca agttcattta accatgataa ttttacccgc ataaaagcct
     2761 cctaaaactg tttaaggaat cgtaaatcat cagtataaaa atgcctgatg tcatcgatac
     2821 cgtaacgaag catcgtaagg cgctcaatgc cgatgccaaa agcaaaacct tgaaattcat
     2881 taggatcata gcccgacatt tttaaaactt gaggatgaac catcccggcc cctaaaactt
     2941 caatccaccc gccgttctta caaagcgaac aaccatgccc tttacatttg aaacaagtaa
     3001 catcgacttc aacgctcggt tccgtaaacg gaaagaacga gggacgaaaa cgaacttcca
     3061 aactggaacc aaacattttt tgcatcaata ccgttagtgt tccttttagg tcggacatgg
     3121 taatacctcg atcaaccacc aaaccttcga tttgcataaa ttggtaagaa tgagtcgcat
     3181 cgtcatagtc acgacgataa actttccccg gacaaataat ttttaccggt tgttgcttgg
     3241 ccgctaaaag cgtccgcact tgaacaggag aagtatgtgt tcgtaataag gtgttgccat
     3301 caatataaaa agaatcctgc atttcccgag cgggatgccc ttttggtaaa ttgagcattt
     3361 cgaagttata caaatccagt tcaacttccg gcccttccgc aatcgcatat cccattccga
     3421 taaaaatgtc ttcaatttct tccgtcattt tagttaaggg atgtttattg cctaaagaaa
     3481 aagctattcc agcaagactg acatcaatcg cttcgctttc taactgggcg atgatttgcg
     3541 cttgttccaa ggcttccgct tttttcgcaa tcgcttcttc aacataattt ttaaaccaat
     3601 taaccttttg accaagagtt ttcttttctt caatgcttaa gtctttcatc cttgcaaaca
     3661 attcctgaat cggacttttt ttacctaaaa aaagtgcttt ggtttcatgt agttcattca
     3721 ttgttttgac cttggctaat tcttccaata aacgctcctg taataactta aattgattat
     3781 ccataaatcc tcctaaaaat aaaaaagcct taactttagt taaggacgaa atcttccgtg
     3841 gtaccacctt agttcaatat tattgcactc atttctttga taacgcaagg ggctttgcgg
     3901 gttggattag aacctctccc aagatgaatt cattagcgtt tctggttaag acttgcagtc
     3961 acgatcctaa ctccctaaga cagaaacgat gccaattact acttctcgat cctcgatttc
     4021 ttgcattata tcacttatta cgaaaatgtg caagtctttc ccaaccgtaa tgtttgactt
     4081 attataatga aaaa
//
LOCUS       DAOSGN010000023         4067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_20012_length_4067_cov_2.603938, whole genome shotgun sequence.
ACCESSION   DAOSGN010000023 DAOSGN010000000
VERSION     DAOSGN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20012_length_4067_cov_2.603938"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PK087_00720"
     CDS             <1..945
                     /locus_tag="PK087_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH17821.1"
                     /translation="VSKGTAVIKVTVADVGEKTLTVTVTEKVVFTLALSGEETVKVGE
                     TITLTATSTKPQAVFVWSSSNDAVATVKDGLVKGVSKGTAVIKVTVADVGEKSMTVTV
                     TEEVVIITPKEYTPAELKDLLEGVLDDYSKGLAGYVKIEAVNGPNVLTSELMFNFNND
                     PGIESMMYTLNGTEVAHVYVKDGIAYMLRNQAKTKAEMTATEEATIINNYGFDKFVQS
                     VAAFYSEVEFYDALTFVSRVDDVLTYNLDLAGYLGDVFVTEGKDSITLKVYLGAEDIV
                     LKVETLIVKGTQNNYTILEFKGLGPQTIPYPVDLDAYE"
     gene            1055..2026
                     /locus_tag="PK087_00725"
     CDS             1055..2026
                     /locus_tag="PK087_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOH17822.1"
                     /translation="MKSINLQAFGKQNISFYLAIEQQLLPKMAEDLFFLWDLNPAIVV
                     GRHQLIASEVNMGFAKKHQLPVYRRPTGGGAVYADEGCFMFSFLSKNTNREQVFFQYL
                     SQIKNFFLTLGLEVELSGRNDLLFLGKKVSGNAFLTNQYGSILHGTILYNTNLEFLVN
                     ALTPDDDKLISKGIKSVRERVINLKDYLNLSMTEVMMQMWSYFSSSQAFLSAADLAGI
                     KKWEAHFLSSAWLNDHQPPYSIRVKKRFAWGLIEAFIDVKRNKIKGLEMVGDFFEKQR
                     ITAWTQSFIGSLYSEAAFLDILKQNDIANFIDGANNHDIMDLIWRKE"
     gene            2023..3105
                     /gene="gcvT"
                     /locus_tag="PK087_00730"
     CDS             2023..3105
                     /gene="gcvT"
                     /locus_tag="PK087_00730"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOH17823.1"
                     /translation="MKKTILYDEHVRLGGKMVEFAGYLLPIEYTGIALEHQAVRDQCG
                     VFDVSHMGEIEISGKDAIAFINYLFTNDVASVEAKRMVYGLMLYPHGGVVDDMMVYKY
                     NNHHLLLVVNAGNKDKDLAWILDHQQAYDVEVVDRSGYYSQLALQGPKAEGILQSLTK
                     YPLHNLRSLTFDHLTMLNHDFLVSRSGYTGADGFEIYGANAHIFELFQLFSRLPEITL
                     CGLGCRDTLRFEASMPLYGHEIDETINPLEAGLNFAVKLDKSFIGSEVLVAAQASGLK
                     RKVVALELIERVIARLGYEVEADGQLIGHVTTGYMIPNTTKSYAFALIASEYAALGTI
                     VGIRIRKNLVKAKVRNKRLLEKKYVR"
     gene            3115..3492
                     /gene="gcvH"
                     /locus_tag="PK087_00735"
     CDS             3115..3492
                     /gene="gcvH"
                     /locus_tag="PK087_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOH17824.1"
                     /translation="MSKVLDNLKYAPTHEWARVEGEFAYIGITDYAQDSLGAIVYVEG
                     GDVGDSVSQFEEFGAVESVKAASDLLSPLSGTIVEINEEVIDEPELINQDPYTHWLIK
                     IEMSDKSEIDNLLDAVSYQKMAK"
     gene            3492..4058
                     /locus_tag="PK087_00740"
     CDS             3492..4058
                     /locus_tag="PK087_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17825.1"
                     /translation="MNKYFPHSEQDIAQMLAKIGVSHLDDLFKDVPSHVLHHGPFRLP
                     QQLSEIEIRDEMAKIAAQNKKLISFLGAGSYDVYTPSVISALTSRQEFLTSYTPYQPE
                     ISQGNLQYIFEFQSMICELTGMDVSNASVYDGATATAEAMFMAVSQTKRNRVLVSATI
                     NPRILAVLKTYAYFRDVDLVMIPMKEYH"
BASE COUNT         1188 a          681 c          923 g         1275 t
ORIGIN      
        1 gtttcgaaag gaacagcggt cattaaagtt acggttgccg atgttggcga aaagaccttg
       61 accgtgaccg taacggaaaa agtcgttttc actttagcgt taagcggtga ggagacagtc
      121 aaagttggcg aaacgattac tttaacggct acctccacaa aaccacaagc ggtgtttgtc
      181 tggagttcgt ctaatgatgc ggtggcgaca gttaaggatg gtttagtaaa gggtgtttcg
      241 aaaggaacag cggtcattaa agttacggtt gccgatgttg gcgaaaagag tatgaccgtg
      301 acggtgactg aggaagtcgt tattatcacc ccgaaagaat atacacccgc cgaattaaag
      361 gatttattgg aaggcgtttt ggatgattat tcaaaaggct tagccggtta tgtgaagatt
      421 gaagccgtca atggtcctaa tgtcttaact tccgagttaa tgtttaattt taataatgat
      481 ccgggcattg aatcgatgat gtatacttta aacggaaccg aagtcgctca tgtttatgta
      541 aaagatggaa ttgcttacat gcttcgcaat caagcaaaga cgaaagcgga aatgacagcg
      601 acggaagaag caaccattat caataattac gggtttgata aattcgttca aagtgttgcg
      661 gctttctact ccgaagttga attctatgat gctttgacat tcgtttcccg tgttgacgat
      721 gtgttaactt ataacctgga cttagcgggc tatttaggtg atgtttttgt gacagaaggc
      781 aaagattcga ttacattgaa ggtttattta ggggctgagg atattgtcct aaaagtggaa
      841 actttaattg taaaaggaac acaaaacaac tacactattt tggagtttaa aggtttagga
      901 ccacaaacaa ttccttatcc cgttgattta gatgcttatg aataatgacc taaaaactat
      961 ttagagaaac ctaaatagtt ttttttattt aaaaagtaaa atgttgtttt aaaaagccag
     1021 cgtttgtgat aaaataaata gggaaatata aaaaatgaaa agtatcaatt tgcaagcttt
     1081 tggtaaacaa aacatctctt tttatttggc aatcgaacag caattattgc cgaagatggc
     1141 ggaagatttg ttttttttat gggatttgaa tccggcgatt gtcgttggac gccatcaatt
     1201 gattgctagt gaagtcaata tgggatttgc taaaaaacat cagcttcccg tttatcgtcg
     1261 tccaacgggc gggggagcgg tttatgccga tgaaggctgt tttatgttta gctttttatc
     1321 taaaaatacc aatcgcgagc aagttttctt ccaatacctt agccaaatta agaacttttt
     1381 tcttactttg ggtttagagg tggaattgtc aggacggaat gatttattgt ttttaggaaa
     1441 aaaggtttcc ggtaatgcct ttttaactaa tcaatatggt tccattctgc atggcacgat
     1501 tttgtacaat accaatttag aattcttagt taatgcgctc acacccgatg atgataaact
     1561 tatttccaaa gggattaaaa gtgttcggga acgagttatc aatttaaaag attatcttaa
     1621 tctatccatg acggaagtca tgatgcaaat gtggtcgtac ttttcatcat cacaagcttt
     1681 tctaagtgct gccgatttag ccggtattaa aaaatgggaa gctcattttt tgtcatctgc
     1741 atggttgaat gatcatcagc caccttattc tatacgggtg aaaaaacgct ttgcttgggg
     1801 tttaatcgag gcttttatcg atgtgaagcg aaataagatt aaaggattgg aaatggtcgg
     1861 tgattttttt gaaaaacaaa ggattactgc ttggacgcaa tccttcattg gtagtttgta
     1921 ttctgaagca gcttttttag atattttaaa acaaaatgat attgctaatt ttattgatgg
     1981 cgctaacaat catgatataa tggacttaat ttggaggaaa gaatgaagaa aacaattttg
     2041 tatgatgaac atgtccgatt aggaggcaaa atggtagaat ttgccgggta tttattgcca
     2101 atcgagtaca caggaattgc tttagagcat caagcggtgc gcgatcaatg cggtgttttt
     2161 gatgtttccc atatgggaga aatcgaaata agtggcaagg atgccatcgc ctttatcaat
     2221 tacctcttca caaacgatgt cgctagtgtc gaagccaagc gtatggttta tgggttgatg
     2281 ctctatcctc atggtggcgt tgttgatgac atgatggttt ataaatataa taatcatcac
     2341 ttgctattag tcgtcaatgc cggcaataag gataaggatt tggcttggat tttagaccat
     2401 caacaagctt atgatgtaga ggttgtcgat cgctccggat attattccca attggctttg
     2461 caaggcccca aggcggaagg catcttacaa agtttaacta aatatccttt gcataactta
     2521 cgttcactta cttttgatca tctaacgatg ctcaaccatg attttttggt ttcccgaagt
     2581 ggttataccg gtgcggatgg ttttgaaatt tatggtgcca atgcccatat ttttgaactg
     2641 tttcagttgt tttctcgtct tcccgaaatt actttgtgcg gtttagggtg tcgcgacact
     2701 ctccgctttg aagcatccat gccgctttat ggtcatgaaa tcgatgagac gattaatccc
     2761 ttagaagcgg gattaaattt tgctgttaaa ctggacaaaa gctttattgg tagtgaggtt
     2821 ttggttgctg ctcaagcttc aggtttaaag cggaaagttg tagctttaga attgattgag
     2881 cgtgtgattg ctcgccttgg ctatgaagtt gaagccgacg gtcagcttat tggtcatgta
     2941 accaccggtt atatgattcc taatacgacc aaatcctatg cctttgcctt aatcgccagt
     3001 gaatatgccg ctttaggaac gattgttggg attagaattc ggaagaatct tgtaaaagct
     3061 aaagtgcgaa acaaacgatt attagaaaaa aaatatgttc gataggagga aaacatgagt
     3121 aaagtattag acaatttaaa gtatgcccct acccatgaat gggcgagggt cgaaggtgag
     3181 tttgcctaca ttggcattac ggattatgct caggatagtt tgggcgccat tgtttatgta
     3241 gaaggtggag atgttggtga ttctgtgagc caatttgaag aattcggggc tgtggaaagc
     3301 gttaaagccg cttcggattt gctgtccccg cttagcggaa ccattgtgga gattaatgag
     3361 gaagttattg atgaaccgga attaattaac caagatccct atacccattg gcttatcaaa
     3421 attgaaatga gtgataaaag cgagattgat aacttgctag atgctgtcag ttatcaaaaa
     3481 atggcgaaat aatgaataaa tattttcctc attcagagca agatattgcg caaatgctgg
     3541 cgaaaatcgg cgtttctcat ttggatgatt tgtttaagga tgtcccgtcc catgtccttc
     3601 atcatgggcc ttttcggcta ccccaacaat tgtccgaaat agagattcgt gatgaaatgg
     3661 caaagattgc cgcccaaaac aaaaagctga tttccttttt gggggccggt tcctatgatg
     3721 tttatactcc gagtgttatt tcagccctaa cttcccgtca agaatttttg acttcctata
     3781 cgccttatca accggaaatt tcccaaggca atttacaata tatttttgag tttcaaagta
     3841 tgatttgtga attaacgggc atggatgtca gcaatgccag tgtttatgat ggggctacgg
     3901 ctacggccga agcgatgttt atggctgttt cgcaaaccaa gcgtaatcgt gtgctggtta
     3961 gtgcaacaat caatccccgc attttagcgg ttttaaagac ctatgcctat tttagggatg
     4021 tggacttagt gatgattccg atgaaggaat atcactaagt ccacatc
//
LOCUS       DAOSGN010000024         3968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_20771_length_3968_cov_3.397393, whole genome shotgun sequence.
ACCESSION   DAOSGN010000024 DAOSGN010000000
VERSION     DAOSGN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20771_length_3968_cov_3.397393"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1287
                     /locus_tag="PK087_00745"
     CDS             292..1287
                     /locus_tag="PK087_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17826.1"
                     /translation="MKKHFSRYIILLVVIMLPLLAAYLVGRYGYYSEKFGQGSWKREF
                     VQDYLTFADTGTTEEQIDKFLKYGINTQYHSYDLVPIYSVQAKNDDNKDLFKIKIYRS
                     LYITTVAGEKVDRVQYLYFIYDIQYLQIREEFGGDSALQREIEEADVPALSVYVYEVN
                     DDDTIVEKDYPSKVAQVDSKLIYDQGADVDYINGKNQDPSKPPITADLNYIYVGFDPM
                     LDVDWSTKTKIEIIAAVKGVTDNDNEDIKTTVFEQILTNYECHPEAIDQTDFVASYQQ
                     KPDNMGYSTWVFGHYLWWIGLITFVACGFITGSFYVVYLSEEQKQQNKNLKKAKK"
     gene            1318..2151
                     /locus_tag="PK087_00750"
     CDS             1318..2151
                     /locus_tag="PK087_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOH17827.1"
                     /translation="MMEIKSIERALITRYRNKLYRPFIKALKHYEMLQENDCIGVCIS
                     GGKDSLTLAKLFQELAKYSDFPITLKYLVMDPGFNEENLDRLKRNAESMGIPIIIKKS
                     DVFQVAYNHGGSNPCYLCARMRRGFLYQFAKEEACNKIALGHHFNDVVETTMLNILYA
                     GCYKTMLPKLKSKNFPGMELIRPLYFVWEKDIINFMKYIGIEAMSCGCKIGDGKMDSK
                     RKEIKEMIAQMKLTNKEVEKNIFRSSENVHVDTVLGWKRQGKRYQFLEEYKHLRDDDS
                     E"
     gene            2213..>3968
                     /gene="ppdK"
                     /locus_tag="PK087_00755"
     CDS             2213..>3968
                     /gene="ppdK"
                     /locus_tag="PK087_00755"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOH17828.1"
                     /translation="MAEKKYVYMFTETDETMKNLCGGKGSNLGGMTKLGLPIPQGFIV
                     TTEACTYYYQNKEQLSAGIVKEIEGALAALEKITEKQFGNPENPLLVSVRSGARVSMP
                     GMMDTILNLGLNDEVVAGLAKTTDNPRFAYDSYRRFIQMYSDVVMGLAKSRFEEIIDE
                     MKQERKIVQDTQFSATDFQEMIARFKAFYQQELHEEFPSDPKTQLFGAINAVFRSWEN
                     PRANYYRKINNIPSEWGTAVNVQAMVYGNMGETSGTGVAFTRNPATGENQIYGEYLIN
                     AQGEDVVAGVRTPSPIAKLHDDLPEVYQQFLEVAKKLEAHFKDMQDIEFTIENGKLYL
                     LQTRSGKRTAQAALQIAVDLFEEGMVTKEKAIMLVQPEQLDNLLHPQFDQKALKSAKK
                     IGEGLPASPGAACGQVVFNVTEALDLTKKGQKVILVRLETSPEDIEGMHVSEGFLTAR
                     GGMTSHAAVVARGMGKCCVSGAHNLEINEAEGYFRLGGQTFKKGDWISLDGSTGAVYG
                     QKIPTVPATISGNFEKFMTWADEIRRLKVRTNADTPADAKQARAFGAEGIGLCRTEHM
                     FFDATRIKAMREMIVSDTK"
BASE COUNT         1296 a          693 c          874 g         1105 t
ORIGIN      
        1 caattaaggt ggaaaaagcc gtattaaaat aggaagatgc caccgtgttt aaggtaatgt
       61 tggtttcgta aatcgaaaca aagccctgtg aatttttaga aaaccataga atatccatac
      121 aactcctcct ttcgccttta gtatacaaca taattcaaaa aaatacaaga aaaacacaat
      181 aaaatatttt tattaattgg ggcgattaat aaactaaaaa caattgaaaa atgaccataa
      241 ctatggtata atgctatcca gttaccaaag gagacctaaa ggagacctgc tatgaaaaaa
      301 cattttagcc gctatataat tttactagtc gtgattatgc ttccgctttt ggctgcatat
      361 ttagtgggaa gatacggcta ttattcggag aaattcggtc aggggtcttg gaaaagagaa
      421 tttgttcagg attatttgac ctttgcagat acggggacga cggaagagca aatcgacaaa
      481 ttcttaaaat atggaattaa cactcaatat catagttatg acttggtacc gatttattcc
      541 gtacaagcga aaaacgatga taataaggat ttatttaaaa taaaaattta tcgcagttta
      601 tatattacaa cagtggcagg agaaaaagtc gaccgcgttc agtatttata ctttatttat
      661 gatattcaat atttgcaaat tcgggaagag tttggcgggg atagcgcttt acaacgcgaa
      721 attgaggaag ccgatgttcc ggccttgagt gtttatgttt atgaagttaa tgatgacgat
      781 acgattgttg agaaagatta tccttcgaaa gttgcgcaag tcgattccaa attaatctac
      841 gaccaaggtg cggatgttga ttacatcaac gggaaaaacc aagatccgtc caaaccgcca
      901 attacggctg atttaaatta tatttatgtt ggctttgatc caatgcttga tgtcgattgg
      961 tcaacaaaga cgaaaataga aatcatagct gcggtcaaag gcgtcacgga taacgataac
     1021 gaagatatta aaacgacggt atttgaacaa atactaacaa attacgaatg tcatccggaa
     1081 gccatcgatc aaacggattt tgtggccagc taccaacaaa aaccggataa catggggtat
     1141 tcaacttggg tgtttggtca ttatctatgg tggattggct taattacctt tgtggcttgc
     1201 gggtttatta ccggttcttt ctatgttgtt tatttgtcgg aagaacaaaa acaacaaaat
     1261 aaaaacctta agaaagcgaa aaaataagat tgttcagtca atcttatttt caagtttatg
     1321 atggaaatta aaagtattga acgagcctta attacccgtt atcgtaataa attatatcgt
     1381 ccgtttatta aggccctgaa acactatgaa atgttacagg aaaacgactg tatcggagtt
     1441 tgtatctccg gaggtaaaga tagtttaacg ctggcgaaac tgtttcaaga actcgccaaa
     1501 tatagtgact ttccgattac gcttaagtat ttagtgatgg accccgggtt taatgaagaa
     1561 aacctcgacc gccttaaaag gaatgcggaa agcatgggga ttcctataat tatcaaaaaa
     1621 tccgatgtct ttcaagttgc ttataatcat ggcggctcca atccctgcta tttgtgtgct
     1681 cgcatgcggc gtggtttttt ataccaattt gctaaggaag aagcttgcaa taaaattgct
     1741 cttgggcatc attttaacga tgtcgttgaa accacgatgc tgaatatatt gtatgccggt
     1801 tgttataaaa caatgttacc gaaactgaaa tcaaaaaact ttcccgggat ggagttaatt
     1861 cgccccttgt acttcgtttg ggaaaaagat attatcaatt ttatgaaata catcgggata
     1921 gaagcgatga gttgcggttg taagattggc gatggcaaaa tggattccaa acggaaagaa
     1981 attaaagaaa tgattgcgca gatgaagcta actaacaaag aagtcgaaaa gaacattttt
     2041 cgcagtagcg aaaatgttca tgtcgatacc gtattaggat ggaaaagaca aggaaaaaga
     2101 taccaattcc ttgaagaata caagcattta agggatgatg attccgagta attttgtttg
     2161 tctttttaaa gttaatatgc tataattgga aagaaaatta aaggaggtca gtatggcaga
     2221 aaaaaaatat gtctatatgt tcacggaaac cgatgaaacc atgaaaaacc tttgtggtgg
     2281 taaaggctct aacttaggcg gaatgacgaa gttaggttta ccgattcccc aaggcttcat
     2341 tgtaactacg gaagcgtgca catattatta ccaaaacaaa gagcaattgt cagccggcat
     2401 tgttaaggaa attgaaggcg cccttgcggc tttggaaaaa attaccgaaa aacaatttgg
     2461 gaatcccgaa aatcccctct tagtatcggt tcgtagcggg gctcgggtgt cgatgccggg
     2521 aatgatggat accattctta atttaggttt gaatgatgaa gtggtggccg gattagcgaa
     2581 aaccaccgat aacccacgct ttgcttatga ctcttatcgc cgctttattc aaatgtactc
     2641 cgatgttgtc atgggtttag ccaagagtcg ctttgaagaa atcattgatg aaatgaaaca
     2701 agagcgtaaa attgttcagg atacgcagtt tagtgctacc gatttccaag aaatgattgc
     2761 gagattcaaa gctttctatc aacaagaatt gcatgaagaa ttcccaagcg atcctaaaac
     2821 ccaattattc ggcgccatta atgcggtgtt ccgttcatgg gaaaatccgc gcgctaatta
     2881 ttatcgcaag attaacaata ttccttccga atgggggacc gctgtcaatg ttcaagccat
     2941 ggtttatgga aacatggggg aaacatcggg gacgggagtc gcgtttacaa gaaatccggc
     3001 gaccggtgaa aaccaaattt atggcgaata tttaattaat gcgcaaggcg aagatgtcgt
     3061 tgcaggcgtt agaacaccat caccgattgc gaaattgcat gatgatttgc cggaagttta
     3121 tcaacaattc cttgaggttg cgaaaaaatt ggaagctcat ttcaaggata tgcaagatat
     3181 cgaatttacc attgaaaacg ggaaactata tttattgcaa acacgaagcg ggaaaagaac
     3241 cgcacaagca gccttacaaa ttgccgttga tttatttgaa gaaggcatgg ttactaaaga
     3301 aaaagcgatt atgcttgtgc aacccgaaca actagataat ttacttcatc cacagtttga
     3361 ccaaaaagcc ttaaagagcg ctaagaaaat tggtgaaggc ttaccggctt caccgggagc
     3421 tgcttgtggt caagtggtct ttaatgttac tgaagccctt gatttaacca aaaaaggtca
     3481 aaaagttatc ttggttcgct tagaaacgag cccggaagat attgaaggta tgcatgtttc
     3541 cgaaggtttt ttaacagccc gcggagggat gacttcccat gctgccgttg ttgctcgggg
     3601 gatgggtaaa tgctgcgttt ccggggccca taatctagaa atcaacgaag cggaaggcta
     3661 tttccgtctt ggcggtcaaa cctttaaaaa aggtgattgg atttctttag atggttcaac
     3721 cggcgctgtt tacggacaaa aaatccccac tgttccggca acaatatccg gaaactttga
     3781 aaagtttatg acatgggccg acgaaatccg tcgcttgaaa gttcgcacta atgctgatac
     3841 accggctgat gctaagcaag ctcgtgcttt tggtgctgaa ggcattggct tgtgtcggac
     3901 tgaacatatg ttctttgatg cgaccagaat taaagcgatg cgggaaatga ttgtttccga
     3961 taccaaag
//
LOCUS       DAOSGN010000025         3935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_21027_length_3935_cov_2.786082, whole genome shotgun sequence.
ACCESSION   DAOSGN010000025 DAOSGN010000000
VERSION     DAOSGN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21027_length_3935_cov_2.786082"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK087_00760"
     CDS             complement(<1..195)
                     /locus_tag="PK087_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012684484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17829.1"
                     /translation="MNELVSPHFKRIYVEITNVCQLACTFCLPLNRPKQFMTTEQFRR
                     VLSEVKPYTDYLYWHVKGEPL"
     gene            complement(188..436)
                     /locus_tag="PK087_00765"
     CDS             complement(188..436)
                     /locus_tag="PK087_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOH17830.1"
                     /translation="MEKLTKQEIETKVKTIINKIRPYLLRDGGDIEFLRIENGIVYVR
                     MMGACVGCANIDVTLKDGIEAILLEEVPGIFGVENVYE"
     gene            complement(498..1649)
                     /gene="ispD"
                     /locus_tag="PK087_00770"
     CDS             complement(498..1649)
                     /gene="ispD"
                     /locus_tag="PK087_00770"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOH17831.1"
                     /translation="MMFDVVLLMAGSGTRTLLPYNKIFYQINGQPLFMFSLEVFLSFP
                     ECARIVLVVKEGEEEKVLAALDSIDQTRIYVVPGGSQRQDSVASGMKICQEETVLIHD
                     GARPCLLKSDVERLLEVVKTAPAAALAIPLTDTIRQKQTSGYRVLDRSTLMAMQTPQA
                     VQRKHYLEALAFAQAEGFYGTDDLELLERYLQIEPVLVFGDARNLKVTSPADLKMVDA
                     YLQGGKRHYKIGHSHDTHRFVFGRPLILGGVTIPCEKGLLGHSDADIVYHVVSEAIIG
                     ALGLGDLGQHFPDTDPSFHNISSSYFVTTAKQLLIDHQYQISNLDITIHLEEPHLKIY
                     KDQMKNNLASLLEIDSCCINVKATRGEGLGFIGQKQGVSAECVVLLYPK"
     gene            complement(1697..3265)
                     /locus_tag="PK087_00775"
     CDS             complement(1697..3265)
                     /locus_tag="PK087_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17832.1"
                     /translation="MLNDIIVFITNNKLTLFIPTLLLVVVFLFGSVLRKPKTPWVSLI
                     VTLLAIGGLVVEILKVVPETFSLFGFSYLFYLPALTLLALIITLIEFLVSQRQYHNRI
                     AIIEAMPSNIETNVYAYLNRQGRLLKLTEGFLDLFTDLISGKQMWFESISRFSCNDEE
                     MTYKKWLAFLKHAPESIFLVNIKLVNDEELNLNLEKKAIFKDEKLVGYILLNQKLTLS
                     EVFKDDVKNDYKKRLKKYLDLLDEPLAYFDQDKNKYILSTQMMRLLNAKESELAPAVF
                     EKLIVPEDLGALSKRPTTEGQAVVQYRLITINGQEWFEENIQHHGNHRYLVLHRSDFT
                     KTKINFKGYPELLSDVNAFIDQNRDFALAFLALKDLPRIVDVLGNDASELVALKYFTK
                     LNASSLSGKIKVYRISKMEYGIIIDRLDNYDLILRDLKNNVSELLGLEIYFNEKKFVL
                     KNAVGIVSSKNVLERTPEAIVKAGFDALFFATDEKYDKRYSIYYAKKPVVETKPEDMK
                     IDLSDQFLDQLLRK"
     gene            complement(3259..3630)
                     /locus_tag="PK087_00780"
     CDS             complement(3259..3630)
                     /locus_tag="PK087_00780"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOH17833.1"
                     /translation="MRIALIAHDKKKPEMIDFAYENRHFLQKHQLFATGTTGKMVMEK
                     TGLPIHCFKSGPLGGDQEIGAQVAKGEIDLVFFFRDPLTAQPHEPDISALLRLCDVYE
                     IPLATNRNTAKMMIKSEVKKC"
     gene            complement(3762..>3935)
                     /locus_tag="PK087_00785"
     CDS             complement(3762..>3935)
                     /locus_tag="PK087_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOH17834.1"
                     /translation="LGTRHRAAIGISQRSDALTVVVSEETGNISVTLDGVINLQLTID
                     RLEEMLDRYLVSK"
BASE COUNT         1239 a          844 c          692 g         1160 t
ORIGIN      
        1 gaggggttca cccttgacat gccagtaaag ataatcggtg tagggcttaa cttcacttaa
       61 cacccgccta aattgttccg tcgtcataaa ctgtttgggg cggtttaagg gcaaacaaaa
      121 agtacatgct aactgacaaa cattggtgat ttcaacatag atccgtttaa aatgaggaga
      181 aacaagctca ttcataaacg ttttcgacac caaaaatacc gggaacttct tctaataaaa
      241 tagcttcaat accgtctttt aaggtgacat cgatgttggc acaaccaaca caagcaccca
      301 tcatgcggac atagacaatc ccgttttcaa tccgtaaaaa ttcaatatcg ccaccgtctc
      361 ttaataaata gggacggatt ttattgataa tggttttgac tttagtttct atttcttgct
      421 ttgttaattt ttccatattc attacctctt ttcatcgtat tataccataa atgtaaaaat
      481 tagttttaga aaatgattta tttcggataa agcaaaacca cgcactccgc cgaaactcct
      541 tgcttttgac cgataaaacc aagcccttcc ccgcgggtgg ctttcacatt aatgcaacag
      601 gaatcaattt ccagcaaact ggcaagattg tttttcattt gatctttata gatttttaaa
      661 tgcggttctt caagatgaat cgttatatcg agattgctga tttgatattg atgatcaata
      721 agcaattgtt tagcagttgt tacaaaataa ctggaggaga tgttgtggaa agagggatcg
      781 gtatcgggaa aatgctgacc aaggtccccg agccccagtg ccccaatgat ggcttcactg
      841 actacatggt agacaatatc ggcatcggaa tgacctaaaa gacccttttc acaggggata
      901 gtgactcccc ctaaaatcaa gggacgacca aaaacgaagc gatgagtatc gtgggaatga
      961 ccaattttat aatgcctttt tcctccctgt aaataagcat ccaccatctt taaatcagct
     1021 ggactcgtaa cttttagatt gcgagcatca ccgaaaacca aaaccggttc gatttgtaaa
     1081 taccgttcca ataactctaa atcgtcggtt ccataaaaac cttccgcttg agcgaaagcc
     1141 aaggcttcta aataatgttt ccgctgcact gcctgcggtg tttgcatggc catcaaggtc
     1201 gagcgatcaa ggacgcgata accggatgtt tgcttttggc gaatggtatc ggtaagaggg
     1261 attgctaaag ccgccgccgg ggccgtttta acaacctcca gcaggcgctc cacatcgctt
     1321 tttaggaggc agggccgtgc tccgtcatga atgaggacgg tttcttcctg acagatcttc
     1381 atgccggaag caacactgtc ttgacgctga ctaccgcccg gaacaacata aatacgggtt
     1441 tgatcaatgc tgtcaagagc agctaacact ttttcttctt ccccttcctt aacaaccaac
     1501 acaatccgcg cgcactcggg aaaacttaaa aaaacttcta aagaaaacat aaacaacggt
     1561 tgaccgttga tttgataaaa tattttgtta taaggaagaa gcgttctcgt tccgcttccg
     1621 gccatcaaca acaccacatc aaacatcata tctcctttta aaaagggcca cttggtaaag
     1681 cggccattaa tctaagctat tttcttaata actgatctaa aaactgatcg cttaaatcaa
     1741 ttttcatatc ttccggtttt gtttccacaa ccggtttttt cgcataataa atgctgtagc
     1801 gtttatcata tttttcatcg gtcgcaaaga acaaagcatc aaacccggct ttcacaatcg
     1861 cttccggtgt tcgttccaac acatttttgg aagaaacaat gccgacagca ttcttcaaaa
     1921 caaacttctt ctcattgaaa taaatctcta aacccaataa ttcggaaaca ttgtttttta
     1981 aatcacgaag aatgagatca tagttatcca gacgatcgat gataataccg tattccattt
     2041 tactaatgcg gtaaactttg attttcccgc ttaaactgga agcattcagt tttgtgaagt
     2101 acttaagagc aacgagctcg ctggcatcat ttcccaaaac atcaacaata cggggcaaat
     2161 ctttaagggc taaaaaggcg agcgcaaaat cacgattctg atcaataaaa gcattgacat
     2221 cgcttaataa ttccggatag cctttaaagt tgattttggt ttttgtgaaa tcggaacgat
     2281 gaagaaccaa ataacgatga tttccgtggt gctgaatgtt ttcttcaaac cactcttgac
     2341 cgttgatggt gattaaacgg tattgaacga cagcttgacc ttccgtcgtt gggcgtttgc
     2401 ttaaagctcc caaatcttct ggaacaatta atttttcaaa aacagccgga gctaactcac
     2461 tttccttcgc attcagtaag cgcatcattt gcgtcgataa aatgtattta tttttatctt
     2521 gatcaaaata agccaacggt tcatcaagta aatcaagata tttttttaat cgttttttat
     2581 aatcattctt gacatcatct ttaaagactt cagacaaggt taatttttgg ttaagcaaaa
     2641 tatagccaac cagtttttca tctttgaaga tggccttttt ttccaaattc aagttcagtt
     2701 cttcgtcatt aactaattta atattaacta aaaaaattga ttccggagca tgttttaaaa
     2761 atgccaacca ttttttgtaa gtcatttcct catcattgca gctaaaacgg ctaatgcttt
     2821 caaaccacat ttgtttacca ctaattaaat cggtaaataa atctaaaaaa ccctcggtta
     2881 atttaagcaa gcgtccttgc cgatttaaat aagcgtaaac attggtttca atattggacg
     2941 gcatcgcttc aataatcgct atgcgattgt ggtattgtct ttgacttact aaaaattcaa
     3001 ttaaagtaat tattaaagca agtagagtta aagctggtaa atagaaaaga tacgaaaagc
     3061 caaacaacga aaaggtctcg ggaaccacct tcagtatttc cacaaccaag ccgccaatgg
     3121 ctaacagcgt gacaatcaaa ctgacccaag gggttttcgg tttccgcaac acagaaccaa
     3181 acaaaaagac cactaccaaa agtaaggtgg gaataaacaa tgtcagttta ttgttagtta
     3241 taaacacaat gatatcattt aacatttttt gacctccgat ttaatcatca tcttagcagt
     3301 attgcgattt gtcgctaacg ggatttcata aacatcgcac aatcttaaca aagccgaaat
     3361 atccggttca tgaggttgag ccgttaatgg atcacggaag aagaaaacta aatcaatttc
     3421 tcctttcgcc acttgggcgc caatttcctg atcgccaccc agcggtcccg atttaaagca
     3481 gtgaatcggc aatccggttt tttccataac cattttcccc gtcgtccccg ttgcaaacaa
     3541 ctgatgtttt tgcaagaaat gacggttttc ataggcaaag tcaatcattt caggtttctt
     3601 tttatcatga gcaattaaag caatccgcat acctgcactt cctttgactt attatatcat
     3661 aaatgggaaa acaatgacaa caaaaattaa caaggctcta gattttccaa accagagcct
     3721 tttaaaacat catcaaggtt attaatcagt aactaatttt attatttgct gactaaataa
     3781 cgatctaaca tttcttctaa acggtcaatg gtcaattgta aattgatgac accatctaaa
     3841 gtaacggaaa tattaccggt ttcctccgag acgaccacgg tcaaagcatc ggaacgttga
     3901 ctgatgccaa tggccgcccg atgtcttgtt cctaa
//
LOCUS       DAOSGN010000026         3827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_21921_length_3827_cov_2.406416, whole genome shotgun sequence.
ACCESSION   DAOSGN010000026 DAOSGN010000000
VERSION     DAOSGN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21921_length_3827_cov_2.406416"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..3074
                     /locus_tag="PK087_00790"
     CDS             279..3074
                     /locus_tag="PK087_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOH17835.1"
                     /translation="MKKIYFLMLAFFVVLGIVGQVNKQNQALHDLPFHSNQLVEVKPI
                     NQASDFRAVWVTSLTGDIASYSNVTQYQTEINKVFAVMEYYNMNVMVFHIRTHNNALY
                     KSSLNPVASYYRNVNFDAWDPIAWIIEQCHLRGMEFHAWLNPYRCSTTFSGTTTQYAA
                     NQPSYNIASNPENLLKTSNGIILNPGEPAVRQFLVDTCMEVIERYDVDAIHFDDYFYA
                     DGVDDTTTRSKYNQAGLSVEDFRRQQVDLFIEKLSQTMRAYNLAHHRLVQLGVAPTGI
                     YRNSSSGVETVYDSNGTLITPIGSTTGGWQHYGSYLFCDTKKWIDNEWIDYIIPQSYW
                     GFTHPTAGYINVMSWWAKVVKYKNVNLYSGMGLYMAGSSNTYSWVTDPMEASKQIDYA
                     KTLPEVQGHCIFSYKHIKAAYDQNPGRYLENMKNVKNNNWTNEVLVPEIRTYEAISLP
                     SVASIAVAKDQNAYVISFPKVDGAKFYALYRGTKPLSFSADELLAVTSGPTAGLEVTY
                     VDEIGSGQYFYGVRPVSITNTLGGTAMASSDVATLRVRFLDDDGSVLKVQYLLSGGQA
                     LAPSNPTKEGFDFVGWDHELSNITESIDIRAQYSVKMLTVTFKDHEQVLATVQVPYGG
                     TAEVSDPVKEGHVFDGWLQSLENIKQNTVIYASFSPFIFTVVFLGRDGLALKTEQVAY
                     GDSAVAPIINDSHFTAWNQSFANVTSDLLITPLFDDAFKLIFKVDDQIIHEVLDAKGP
                     FQVPSIPFKEGHDQQPPVWSITDFSTITEDTIITPIYYINTFSVTFLVLNNKVLSTEI
                     VKWQQAAPAPTNTAENGYSFQAWNQDFSTVTADATITAIYDINKFTVTFLGLNGKVLS
                     TEIVNWQQGATAPTNTAESGYSFQGWDRDFSCVSEDMTVKGRYLASSTNSCSGLNLPQ
                     LTYLLVALAWLLWKRRK"
     gene            3184..>3827
                     /locus_tag="PK087_00795"
     CDS             3184..>3827
                     /locus_tag="PK087_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOH17836.1"
                     /translation="MIVFKSLFDAGYKELKRNRKIADKIDALALQYQAMTDEELKAKT
                     PYFREQLAQGKTIDDLLIDAFATVREASTRVLKMTPFYVQLLGALALHGGNIAEMKTG
                     EGKTLTSTLVAYLNALPGDGVHIVTVNEYLASRDAKEMGELFTWLGLTVGLNLRELSP
                     EEKKAQYACDITYSTNNELGFDYLRDNMVIYKEQMVQRPLNFAIVDEVDSILID"
BASE COUNT         1114 a          693 c          823 g         1197 t
ORIGIN      
        1 acagcgaaac taaaaaagtt agcgttgttt atttgcgtga agaccaaaat tacggcatta
       61 ttgagactaa ttattaattg aacaaaagat aattattaac cagttagttt aaaaatatct
      121 atggctaagc tactttcgaa agaaaaagaa agaagaacca cggatggatt tttcgtaaat
      181 gttaaatagc gacagtgtcg ctatttttat taaaaaggct tggggaataa cgggaactat
      241 gttataatta ttttagtttt tttaaaggaa tcgataatat gaaaaaaatt tatttcttaa
      301 tgctggcttt tttcgtggtt ttgggaatcg ttggccaagt taataaacaa aaccaagcgc
      361 ttcatgattt accttttcat agtaatcaac tggtagaagt gaaaccgatt aaccaagcca
      421 gcgactttcg tgctgtttgg gtgacaagct taaccggtga tattgccagt tattccaatg
      481 ttactcagta tcagacggaa attaataagg tctttgcagt gatggaatat tataatatga
      541 atgtgatggt cttccatatt cgcactcata acaatgcctt atacaaatct tccctcaatc
      601 ccgttgccag ttattaccgc aacgttaatt ttgatgcttg ggatccaatt gcttggatta
      661 tcgagcaatg ccatttacgt ggtatggaat tccatgcttg gcttaatccc tatcgttgtt
      721 cgacgacttt ttccggaaca acgacccaat atgctgctaa tcaaccttcc tataatattg
      781 cttccaatcc tgaaaactta ttgaaaacat caaatgggat tatcttaaat cccggtgaac
      841 cggcggttcg tcagttttta gttgatacct gtatggaagt cattgaaagg tatgatgttg
      901 atgcgattca ctttgacgat tatttttatg ccgatggtgt tgatgatact acgacccgtt
      961 caaaatacaa tcaagccggt ttgagtgtgg aagatttccg tcgccaacag gtggatttgt
     1021 ttattgaaaa actatcacaa acgatgcggg cttacaattt agctcaccat cgcctcgtac
     1081 aattgggggt ggctcccacc ggaatttatc gtaacagcag ctcgggtgtg gaaaccgttt
     1141 atgacagcaa tggcacccta atcaccccga ttggttcaac taccggggga tggcaacatt
     1201 atggtagtta tttgttttgt gatactaaaa aatggattga taatgaatgg attgattata
     1261 tcattcccca aagttattgg ggatttaccc atccaactgc cggttatatc aatgtcatgt
     1321 cgtggtgggc gaaggttgta aaatacaaaa atgtcaattt gtattccggg atgggtttat
     1381 atatggccgg gtcttccaat acttattcgt gggttacgga tcctatggaa gcttccaaac
     1441 aaattgatta tgccaagaca cttcccgagg ttcagggtca ttgtattttt agttataagc
     1501 atattaaagc tgcttatgac caaaaccccg gtcgctattt ggaaaacatg aaaaatgtta
     1561 agaataataa ttggacgaac gaagtgctcg ttccggaaat ccgcacttat gaagcgattt
     1621 cgcttccttc cgtggctagc attgctgttg cgaaagacca aaatgcttat gttatcagtt
     1681 ttccgaaagt cgatggcgcg aagttttatg ctctttaccg cggaacaaag cctcttagct
     1741 tttcggcgga tgaattgctt gctgtaactt ccggaccgac agcgggattg gaagttactt
     1801 atgtcgatga gatcggtagt ggtcaatatt tttatggtgt ccgaccggtt tcaataacta
     1861 acactttggg tggaacagca atggcttcga gcgatgttgc gactcttcgg gttcgtttcc
     1921 tcgatgatga tggttccgtc ttgaaagttc aatatctttt aagtggaggt caggcgcttg
     1981 cccccagcaa tcccaccaaa gaaggttttg attttgtagg ttgggaccac gaattaagca
     2041 atattactga aagtattgat attagagctc aatatagcgt taaaatgcta actgttactt
     2101 ttaaggatca tgaacaagtt ttagcaaccg ttcaagttcc ttatggaggc actgcggaag
     2161 taagtgatcc ggttaaagag ggtcatgttt tcgatggctg gctccaaagt ttggaaaaca
     2221 taaagcaaaa taccgttatt tatgcttcgt tttcaccgtt tatttttacg gttgttttct
     2281 taggtcggga tggtttagct ctcaaaacag aacaggtggc ttatggcgat tcagctgttg
     2341 cgccgattat caacgacagt catttcaccg cttggaatca aagttttgct aatgtcacta
     2401 gtgatttatt gattactccg ctttttgatg atgcttttaa attaatcttt aaggttgatg
     2461 atcaaattat ccatgaagtt cttgatgcga aaggaccttt ccaagttcct agtattcctt
     2521 ttaaagaagg gcatgaccaa caacctccgg tttggagcat tacggatttt tcgacgatta
     2581 cggaagatac tatcatcacc ccgatttatt atatcaatac cttttccgtc acctttttag
     2641 tcttaaataa caaagtattg agtacggaaa ttgtaaaatg gcaacaagca gcccctgcac
     2701 cgacgaatac cgctgaaaat ggctactcat tccaagcttg gaatcaggat ttttcgacag
     2761 ttaccgccga tgccactatt acggccattt atgatatcaa taagtttacc gttacctttt
     2821 taggtttgaa tggtaaagta ttgagtacgg aaattgtcaa ttggcaacaa ggggctaccg
     2881 ctccgactaa taccgccgaa agtggctact cattccaagg ttgggatcgg gatttttctt
     2941 gtgtaagcga agatatgacg gtgaagggac gatatcttgc ctcaagcact aactcttgtt
     3001 cagggttaaa tttaccccaa ttgacttatt tgcttgtggc tttggcttgg ttattatgga
     3061 aacgaagaaa ataatgtgaa ggtgaaaccc caagtttcac cttttttcca agttttaaat
     3121 aacttgtaaa aaggggaaaa atttgatatt atatttttaa gatatttaat taagaaggga
     3181 agtattattg tgttcaaaag tttatttgat gctggatata aagaattaaa acgcaatcgc
     3241 aaaattgctg ataaaattga tgctttggct ttgcaatatc aagctatgac agatgaggag
     3301 ttaaaagcga aaacgcctta ttttcgcgaa caattagccc aagggaaaac cattgatgat
     3361 ttactcatcg atgcttttgc gacggttcgt gaagcctcaa cacgagtttt aaaaatgacc
     3421 cccttttatg tgcagttgtt gggggctttg gcgctccacg gtggcaatat tgccgagatg
     3481 aaaaccggtg aaggcaaaac cttaacctca actttagtag cgtatttaaa cgctttaccg
     3541 ggtgacggag tccatattgt tacggtcaac gaatatttag ctagtcgtga tgcgaaggaa
     3601 atgggggaac tgtttacttg gttagggctc acggttggct taaacttgcg ggaattaagc
     3661 ccggaagaaa aaaaggctca atatgcctgt gatatcacct attccacaaa caacgaactg
     3721 ggtttcgatt atcttcgtga caatatggtc atttataaag aacaaatggt tcagcgaccg
     3781 cttaattttg cgattgtgga cgaagtggac tcgattttaa tcgatga
//
LOCUS       DAOSGN010000027         3823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_21958_length_3823_cov_3.200902, whole genome shotgun sequence.
ACCESSION   DAOSGN010000027 DAOSGN010000000
VERSION     DAOSGN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21958_length_3823_cov_3.200902"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PK087_00800"
     CDS             <1..247
                     /locus_tag="PK087_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17837.1"
                     /translation="KNTLLIKALNKEEATMGKYTDNDLHAISIKPTYGRINGKNIINN
                     IQKFFPMDFTKKNLYKFPCEWDNSQKYLRVYFDKEVH"
     gene            247..732
                     /locus_tag="PK087_00805"
     CDS             247..732
                     /locus_tag="PK087_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOH17838.1"
                     /translation="MLFLRAFEPVDWVMMVAWIIVFIVTLIIELETTNLTTIWFCLSS
                     VVTIICGVLFAGPYLQIVIFVVLSLVLILATRPLTRKMMRKDIIKTNTDRLIGMIAVV
                     TKEIHPNEIGEVKVENNLWRAINNDDLSFAVGEKVLIDAIVGIKLVVSKVDGNQNLEI
                     L"
     gene            747..1700
                     /locus_tag="PK087_00810"
     CDS             747..1700
                     /locus_tag="PK087_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOH17839.1"
                     /translation="MFHSLFLVNLGGIIALLIVLFIALIVVATSFKIVPQANAYIIER
                     LGSYHTTWGTGLHFLIPFIYRVAKKISLKEQVIDFDPQPVITKDNVTMQIDTVIFFTI
                     TDGKAYAYGVENPLMAIEKLTATTLRNIIGELDLDGTLTSRDIINVRMRDILDVATDP
                     WGIKVNRVELKNILPPKDIRDAMEKQMRAERERREAILRAEGEKRAAILQAEGFKEST
                     ILQAEAKRQALINEAEGRKEAQVLEAIGEAQAILEVQTAKAKSIELIRKAGADQAVLT
                     IKAYEALAEMANGQATKIIVPSNLQGLAGTLASLVEVAKDK"
     gene            1785..2693
                     /locus_tag="PK087_00815"
     CDS             1785..2693
                     /locus_tag="PK087_00815"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH17840.1"
                     /translation="MTELELRFIEWLRSIATPTTDAIFEFITSLAEQSILIAILVIIY
                     FFYSKKTGQNIAFTIFFSMLLNNSLKGLVGRVRPFKHPQATFEPARVETATGASFPSG
                     HTQNATVAYTLFSRLFKSKGVKWVAAILIALVGISRLVLGVHYPSDVLAAIVFGLGSV
                     WLGSYLHKRYEDDFQKQIVLYALMAVFFLPFVFIFWRKDYNDIVLFKDFYSAYAFYLG
                     YVVAIYFEKKVVDFDCTQTIKVRLIRTILAVVVVIAIQFGLKLAFPEHSIFFDMLRYF
                     LLALVTLGIYPILTKKWLFYKTEKKG"
     gene            complement(2702..>3823)
                     /gene="mnmE"
                     /locus_tag="PK087_00820"
     CDS             complement(2702..>3823)
                     /gene="mnmE"
                     /locus_tag="PK087_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOH17841.1"
                     /translation="EDVVEISCHGGVFVTTQILELLLEKGCRMAEPGEFTKRAFLNGR
                     IDLTQAEAVADIIDAQTQSSLKMANIGLRGDVKDMIMRFRNQLINMIAKIEVNIDYPE
                     YEDEEQITAEILKPTIMHLQSQLADIIEKSQVSAILKHGIKTAIIGKPNVGKSSLLNA
                     LLREDRAIVTDIAGTTRDSIEATVNLGGLVLNIFDTAGIRDTTDIIEKIGVEKTKKII
                     DQVSLIILVFDYNQELDNNDEALLKLTKNKERIIVVNKQDLKKRLDTSRLPNHLLISS
                     FNEEDIIRLEEQIRSTCHITDLANLDNTFIGNARHIAKLKQAYQSLGDAKTSLNNHQP
                     VDIINLDLRAAWQALGEIIGETIDEALVHELFSKFCLGK"
BASE COUNT         1095 a          773 c          735 g         1220 t
ORIGIN      
        1 caaaaatact ttgctaatta aagcccttaa taaggaagaa gcgactatgg ggaaatacac
       61 agacaatgat ttgcatgcca tttccatcaa accgacctat gggcggatta atggcaagaa
      121 catcattaac aacattcaaa agtttttccc gatggacttc acaaagaaaa atctgtataa
      181 attcccttgc gagtgggata attcgcagaa gtatttacgc gtttattttg ataaggaggt
      241 tcattaatgt tatttttaag agcctttgaa cccgttgatt gggtcatgat ggtcgcttgg
      301 attatcgttt ttatcgtgac tttgattatc gaattagaaa caaccaacct aaccacgatt
      361 tggttttgtt tgagttcggt tgttaccatt atttgtggag ttttgtttgc cggtccttat
      421 ctgcagattg ttattttcgt ggttctttcc cttgttttaa tcttagcaac acgcccatta
      481 acgagaaaaa tgatgcggaa ggatattatt aaaaccaaca ccgatcgcct catcggcatg
      541 attgcagtgg tcacaaaaga aattcatcct aatgaaatag gggaagtgaa agtggaaaat
      601 aatttatggc gcgcgattaa caacgacgat ttaagcttcg ctgttgggga gaaagtttta
      661 attgatgcga ttgtgggaat aaaattagtt gtttccaaag tcgacggaaa ccaaaattta
      721 gaaatattat aaaaaggaga agaaaaatgt ttcattcact atttttagtt aatttgggcg
      781 gcataattgc tttgctcatt gttttgttca ttgctttaat tgttgtggcc accagtttta
      841 aaatcgttcc gcaagccaat gcttatatta ttgaacgatt gggttcctat catacgactt
      901 ggggaaccgg tttacacttt ttgattcctt tcatttatcg ggttgcgaaa aagatctctt
      961 taaaagaaca agttattgac tttgatcccc aacctgtgat taccaaagat aatgttacaa
     1021 tgcaaattga taccgttatt ttctttacca ttactgacgg aaaagcctac gcttacgggg
     1081 tcgaaaaccc cttaatggcg attgaaaaat taaccgccac cacccttcgt aacatcattg
     1141 gcgaattgga cctcgacgga actttaacca gccgtgatat tatcaatgtg cgcatgcgcg
     1201 acattttaga tgttgccacc gacccctggg gcattaaagt taaccgcgtg gaattaaaaa
     1261 acattctccc gccaaaagat attcgcgatg ccatggaaaa acaaatgcga gctgaacgcg
     1321 aacgccggga agcaatttta cgagcggaag gggaaaaacg agccgccatt cttcaagcgg
     1381 aaggattcaa agaatcaacc atcttacaag ccgaagcaaa acgacaagct ttaattaatg
     1441 aagcggaagg acgcaaagaa gcgcaagtcc tagaagccat tggtgaagcc caagccattt
     1501 tggaagtgca aacagccaaa gccaaaagta ttgaattaat tcgcaaagcc ggagccgatc
     1561 aagccgtcct taccattaaa gcttatgaag ctttagcgga aatggccaac ggccaagcta
     1621 ctaagattat tgttccttcc aatttacaag gattagccgg aacactagcc tccttggtag
     1681 aggttgcgaa agacaaataa agttgttcaa gtgggaaacc gcttgaactt tttctttaca
     1741 atcctcttcg tccatattac aatatgaata tcaaggagga ttccatgaca gagttagaat
     1801 tgcgttttat tgaatggtta cgaagtattg ccacccccac aacagatgct atctttgagt
     1861 ttattacctc gttagctgaa caaagcatcc tgattgctat cttagtaatt atttatttct
     1921 tttattccaa gaaaactggg caaaacatcg cctttaccat tttcttttcg atgttgttaa
     1981 acaattcctt aaaaggttta gtcggtcgag tgcgcccctt taagcatcca caggccactt
     2041 ttgaaccggc acgagtggaa accgccaccg gggcttcctt ccccagtgga catacccaaa
     2101 atgctaccgt tgcttatact ttgttttctc gtttgtttaa atcaaaggga gtaaagtggg
     2161 ttgccgctat tttaattgcc ttagtcggca tttcccgctt agtactcggt gttcattatc
     2221 caagcgatgt tttggcggca attgttttcg gtcttggttc ggtttggctt ggttcatacc
     2281 ttcacaaacg gtatgaagat gattttcaga agcaaatcgt gttatacgct ttgatggctg
     2341 ttttcttcct cccttttgtt tttatctttt ggcgaaaaga ttacaacgac attgttttat
     2401 ttaaagattt ttattcagcc tatgcttttt atttaggtta tgttgtggcc atctattttg
     2461 aaaagaaagt tgttgatttt gattgcacac aaacaataaa agtgcgctta atccgcacaa
     2521 ttttagccgt tgttgttgtg attgccatcc aatttggctt aaagctcgcc ttcccagaac
     2581 attccatttt ctttgatatg ttacgttact ttttactggc ccttgtcact ttgggaattt
     2641 atccgattct taccaaaaaa tggctgttct ataaaactga aaaaaagggt taaaaccctt
     2701 tttatttacc taagcaaaac ttggaaaaca gttcgtgcac caaagcttca tcaatcgtct
     2761 caccgatgat ttctcccaaa gcttgccaag cggcccttaa atctaaattg atgatatcaa
     2821 ccggttggtg attgtttagg ctcgttttgg catcccctaa actttggtat gcttgtttta
     2881 gtttcgctat gtgccgagca ttgccaataa aggtgttgtc gaggtttgct aaatccgtaa
     2941 tgtggcaagt actgcggatt tgttcttcca aacgaataat atcttcttca ttaaaggaag
     3001 aaattaataa atgattcggt aagcgacttg tatcaagtcg tttttttaaa tcttgtttat
     3061 taacaacaat aattctttct ttattcttag ttagctttaa aagcgcttca tcgttgttat
     3121 caagctcttg gttgtaatca aaaacaagaa taatcaagga aacttggtca atgatttttt
     3181 tggttttttc gacaccgatt ttttcaataa tgtcggtggt atctctaatc cccgccgtat
     3241 caaagatatt gagcactaag ccccctaaat taacggtcgc ttcaatggag tcacgagtcg
     3301 tcccggcaat atcggtcaca atcgctcggt cttcccgtaa taaagcattt aataaactgg
     3361 atttcccgac attgggttta ccgatgatag ccgttttgat accgtgcttc aaaatagcgg
     3421 aaacttggct tttttcaatg atgtccgcca attgcgattg taaatgcatg atggtcggtt
     3481 ttaatatttc cgctgttatt tgttcctcat cttcatattc cggataatca atgttaactt
     3541 caatcttcgc aatcatatta attaattgat tgcgaaaacg cattatcatg tcttttacat
     3601 ccccgcgcaa accgatattg gccattttta aactgctttg cgtttgtgca tcaatgatat
     3661 ccgccaccgc ttccgcctga gttaaatcaa tccgtccatt taaaaaggcc cgtttagtaa
     3721 attcgcccgg ttccgccata cggcagccct tttccaaaag caattctaaa atttgggtag
     3781 tcacaaaaac gccgccatga cagctaatct ccaccacatc ttc
//
LOCUS       DAOSGN010000028         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_22010_length_3817_cov_3.534556, whole genome shotgun sequence.
ACCESSION   DAOSGN010000028 DAOSGN010000000
VERSION     DAOSGN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22010_length_3817_cov_3.534556"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PK087_00825"
     CDS             <1..612
                     /locus_tag="PK087_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17842.1"
                     /translation="RLKMTVRVVRLVGNSLFVEDLEPTLNADTGLSTKAGIFLYHSFV
                     SGLGKYLPGYVISFECQASDDETYGTQLVNPKNLRTVSKTSEYSITDIGNEVTNLKNY
                     EGFVVRVLGFKVNKVGKESDTGAFTIYGTMRNGAELMVRVDADANPKIAFDYPQIGSR
                     YDVIGGVSKYVNAYEGNAVYYQLKLGNLTEQTMNDFVLSENQK"
     gene            643..3444
                     /locus_tag="PK087_00830"
     CDS             643..3444
                     /locus_tag="PK087_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOH17843.1"
                     /translation="MTFLKNNVKKIAVLLFALLVGFSLSACSSKEDTQKVQEGLASIA
                     VGNLNNLKESFELRATTIQHDLPITWTVECQDNSAKIETVDGKIMVIITRSEYSEDAN
                     KVQNNPWGEATLKATVTVGKQTGTRDWDMFIKPGDKPVENKLTLAAVKAEAMAKDTAV
                     LVEGTVFYVKKDGFMIIDKTETMFVYTNSVPNASIVPGAVVEVEGKKGVYYSMPQIAS
                     PTVKVKTPAPATGFDYSIAVESDVATVEAKPSTNPLNFTKLYKVSGKVESVELYSCKL
                     AIKDTTTNGIIYIHYDSTEAALNELTAKVGSFVSLVVINYEFHSSYKVWRYMAVAGTV
                     KDAEAPVISDAEIVTNSKNELTAAFNKKSFAFDLVFPAKASAGATLTWTSSNTGVIAN
                     DGKFTMPTSDTTVTLTVKVTSNAVSENLSLTVTAKALTKQSVSQVITALNTAAASGKT
                     IDFVLVEGIIVGKDKSDYYYLADADAAIYVRQKLSADNLKVGDSVRVIGKAQVYLNSD
                     KEYTRQISGNYKVVKLDDQTHACPLTLIDVALTDFDFTITKDNFLTAVPAEELYGKLV
                     RFELFVKKVVEGSYTNYYLAESMDEGAAKIKVHHNSANLSEIEKLEGSKVKVTGIIYN
                     YVIATGWSLGFLGREGDIEVPETLTEAEKVAIAKTEIDAIVKEGDNVTKDLGFITETA
                     KATIPGAKYVWTTNDLTTLTAAGVFTAPEADKAVKITVSLFLDGNTAGTASQVWEINV
                     TAKAAVVQAPASVIISQIYGGGGNSGAVYTNDFIVLYNTTDKAVDLTGWVLFYASSTG
                     EFKLDSELSGYVQSAQLSGSIAAHGFYLVQGGAGKTVTDKPLPLTPDATSTFNLSGTK
                     GKLALCNSGDKPVDASSPNVVDFVGYGAADLYEGSGPTPAPSNSTAVVRPTLTDTNDN
                     AADFVTAEPVPKNSTSPAQ"
     gene            3613..>3817
                     /locus_tag="PK087_00835"
     CDS             3613..>3817
                     /locus_tag="PK087_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17844.1"
                     /translation="MLKSRKIMGMIFLLVMTLLFSGCHFGSSKTTITSIEVDSSTLEA
                     SYDVETFDITSIKIKVNYSDGTNK"
BASE COUNT         1184 a          692 c          821 g         1120 t
ORIGIN      
        1 cgtttaaaga tgacggttcg cgtggttcgc cttgttggta atagtttatt cgtagaggac
       61 ttggagccga ctttaaatgc cgacaccggg ctttcaacca aggccggaat cttcctttat
      121 cattcttttg tgagtggttt gggtaagtat ttaccgggtt atgtgatttc gtttgagtgt
      181 caagcgtcgg atgatgaaac ttatggaacc caactggtta atccgaagaa tttacgcacg
      241 gtttctaaaa ccagcgaata cagcattacc gatattggca acgaagtaac taatttgaaa
      301 aactatgaag gttttgtggt ccgcgtttta ggttttaaag taaataaagt cggtaaagaa
      361 tccgacacgg gagcttttac catttacgga accatgcgca acggtgcgga actgatggtc
      421 cgggttgatg ctgatgctaa tcccaaaatc gcctttgatt atccgcaaat cggttctcgt
      481 tatgatgtta ttggcggtgt cagcaaatat gttaatgctt atgaaggcaa cgctgtttat
      541 tatcaattaa aactcggtaa tttaaccgag caaaccatga atgattttgt tttaagtgaa
      601 aatcaaaaat aattagtcaa aagaaagagg aggaaataac ttatgacttt tcttaagaat
      661 aatgtgaaaa aaatagcggt gctattattt gcattattag ttggtttttc tctttctgct
      721 tgtagcagta aagaagatac ccaaaaagta caagaaggtt tagcatcgat tgcggtaggt
      781 aatttaaaca acctcaagga atcgtttgaa ttaagagcaa caactattca acatgacttg
      841 cccattactt ggacagtaga atgccaagac aattccgcca aaattgaaac tgttgacgga
      901 aaaattatgg tcattattac cagaagtgaa tattccgaag atgctaataa agtccaaaac
      961 aatccgtggg gggaagcaac cttaaaagcg acggtgaccg tcggcaaaca aacagggacg
     1021 agggattggg atatgtttat taaaccgggt gataaaccgg ttgaaaacaa attgacctta
     1081 gcagcagtta aagcggaagc gatggctaaa gacaccgctg ttttagtgga aggcacagtt
     1141 ttttatgtga aaaaagacgg ctttatgatt attgataaaa ccgaaacaat gttcgtctat
     1201 accaactcag ttccgaatgc cagtatcgtt ccgggtgcgg ttgtggaagt agaaggcaaa
     1261 aaaggcgtct attattcaat gccgcagatt gcttcgccaa ctgtaaaggt taaaacgccg
     1321 gccccggcaa caggatttga ttactcaatt gctgttgaaa gtgatgttgc gaccgttgaa
     1381 gccaaaccct caactaatcc actaaacttt accaaacttt ataaagtaag cgggaaagtg
     1441 gagtccgttg aactttattc ttgtaaactt gctattaagg acacaaccac taatggcatt
     1501 atctatattc attatgattc caccgaagca gcattaaatg aactaacggc aaaagtcggc
     1561 agttttgtta gccttgttgt tattaattat gaattccatt catcctataa agtatggcga
     1621 tatatggctg ttgcgggaac cgttaaagat gctgaagccc cggttatttc ggatgcggaa
     1681 attgttacca acagcaaaaa cgaattaacc gccgccttta acaaaaaatc atttgctttt
     1741 gatttagtgt tcccggcgaa agcatcagcc ggtgccacct taacttggac atcaagcaat
     1801 accggagtta ttgccaatga tggcaaattc acaatgccaa ccagtgacac aactgtcact
     1861 ttgacggtta aagttacttc caatgcggtt agtgaaaact taagcttaac agttacagcc
     1921 aaagccttga ctaaacaaag cgttagccaa gttatcacgg ctttaaatac tgccgctgct
     1981 tccggtaaaa ccattgactt tgttttggtt gagggcatta ttgttggtaa ggataagagt
     2041 gattattatt atcttgcgga tgcggatgcg gcgatttatg ttcgtcaaaa attatctgct
     2101 gacaatttaa aagtcggtga ttccgttcgc gttatcggta aagcacaagt ttaccttaat
     2161 tctgataagg aatacacccg tcaaatcagt ggcaattata aagttgtgaa acttgatgac
     2221 caaactcatg catgcccatt aacattaatc gatgtcgcct taacggattt tgattttacg
     2281 attacgaaag ataatttctt aactgcggtt ccggccgaag aattgtatgg aaaattagtt
     2341 cgttttgaat tatttgttaa gaaagttgtt gagggctcat atactaatta ctatcttgcc
     2401 gagtcgatgg atgaaggcgc tgccaaaata aaagttcatc acaattccgc caacttaagc
     2461 gaaattgaaa aattggaagg aagcaaagta aaagttacgg gtattattta taactatgtg
     2521 attgcgaccg gttggagttt aggcttctta ggtcgtgaag gtgatatcga agttccggaa
     2581 actttaacgg aagcggaaaa agtggcgatt gcgaaaaccg aaattgatgc gattgtaaaa
     2641 gaaggcgata atgttaccaa ggatttaggt ttcattacgg aaactgctaa agcaacaatt
     2701 ccgggcgcta aatatgtttg gacgactaat gatttaacaa cccttactgc ggccggtgtc
     2761 tttactgctc ctgaagctga taaagccgtt aaaattactg ttagtttatt cttagacggg
     2821 aacacggccg gaacagcttc tcaagtgtgg gagattaatg ttacagctaa agcggctgtt
     2881 gttcaagctc cggcttcggt tatcatcagt caaatttatg gtggtggcgg gaactcaggt
     2941 gctgtttata ccaatgactt tattgttctt tacaacacaa cggacaaagc agttgattta
     3001 actggatggg tgcttttcta tgcatcttca actggtgaat tcaaattaga ttcagaactc
     3061 tccggttatg ttcaatcagc ccaattgtca ggttccattg cggcacacgg cttttatcta
     3121 gttcaaggcg gtgccggaaa aaccgttacc gataaacccc tacctttaac tcccgatgct
     3181 acctccacct ttaatttaag cggaactaaa ggaaaactag ctttatgtaa cagtggtgac
     3241 aaaccggttg atgcaagtag tccgaatgtc gttgattttg ttggctatgg tgcagctgac
     3301 ttatacgaag gatcgggacc aacacctgca ccatctaatt caacagcggt tgttcgccca
     3361 accttaacgg atacgaatga taacgctgcg gactttgtga cagcggaacc tgttcccaag
     3421 aattccacaa gccctgctca ataaacaaac ttaagtcgtt tgggggaaga tttaatcttt
     3481 ttcaaaatac gatataaaaa ttacgaaaca ccttggtttt ataaatcagg gtgtttttat
     3541 ttgttttatt aaaaaaagtt actcctatat tttaaatggt gctataatag tattgatata
     3601 tgggagggac atatgcttaa gtcaagaaag ataatgggaa tgattttttt gttagtcatg
     3661 acccttttat tttcaggatg tcattttggt tcaagcaaaa caacaattac ttccattgaa
     3721 gttgattctt caacgctgga agcatcgtat gatgttgaaa cttttgatat aacaagcatt
     3781 aaaattaaag tcaattattc cgatggaaca aataaac
//
LOCUS       DAOSGN010000029         3781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_22333_length_3781_cov_3.167740, whole genome shotgun sequence.
ACCESSION   DAOSGN010000029 DAOSGN010000000
VERSION     DAOSGN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22333_length_3781_cov_3.167740"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PK087_00840"
     CDS             <1..182
                     /locus_tag="PK087_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17845.1"
                     /translation="GGITKRKEAFLNELNVAVNRIVDPNYWGSTVLSIGRFMNDGGLI
                     GALVHHLNQRETVQN"
     gene            complement(206..1555)
                     /locus_tag="PK087_00845"
     CDS             complement(206..1555)
                     /locus_tag="PK087_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH17846.1"
                     /translation="MKYDVIVCGAGPSGIVAAISAKRNHAQVLLVETSGLLGGNSVLS
                     LVGPWMTFHNHQDQIIKGIGEEIVKRLQAKGYSFGHLPDPLGFADSITPVDVEGIKEV
                     FFDYIREEGIDLLLHAFVCDVISEQNTILGVKVATKSGFLDLYGTIVIDATGDGDVCA
                     LMGCETVFGRQSDHLAQPMSMLFSVNNVDLPRLKAFLKANADDVIMKDDYDYHYLAIA
                     GFFKKVQEAKDAGEFDLIRDRVLLFQDVQAKQVSINMTRVQQLSGIDAFELTKAEMEG
                     RLQIKKAFAFLKKYIPGFENSMIVRTPYRIGVRETRHVIGEYIMQVEDILNRREFDDS
                     ICLSAFPIDIHSPHSEQLELFEQSEDKCYEIPLRVLIPKGKNNLVVTGRCVSATHEAN
                     ASLRVTPSVMALGEAAGVLAALCVQRGLTPKDLDYRLVQKQLVKQGQIIKKVALKKS"
     gene            complement(1557..3008)
                     /locus_tag="PK087_00850"
     CDS             complement(1557..3008)
                     /locus_tag="PK087_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOH17847.1"
                     /translation="MKITVIPLDSRPCNTVWLNDLAKMAGYELTMYPRALCGDLLVGA
                     NFDQMLAWLDDEITDTDYLIVAADGLCSGGLVQARLGQIDAFAVMDRLEVFSEYKKRF
                     PHLKIYVFDTIMRTSITALNKETEKYWGKMNEYSRLLGRTYFFNDEKEKAKLQELLQE
                     IPADMIATYTKARQVKHELNKFFLDLVNQQIVDYLLLLQEDSMPYGVQKIEQEVLQRM
                     IKHQQLEKRVKFYNGTDEGGVVLLGKIILEIKKISPSLFVHLPHPDVLAKVMLFEDQL
                     FSENLKQMLETMGFKTTDSWEQADFILSIYAEARNINLDTNSTIPILPTKDNQYHCYI
                     KELNQFLHSHRPVALVDLLFPNGGSWDLLLDIDLKKLQVYSAWNTASNAMGSALCEIA
                     AKIANPQKNNFKFRNERFLDDCLYQYIVRRTVNEKYLKKGYNVYHLGVNSDEVLSEIR
                     MLMQQYDDWLGITNYRITLPWHRTFEAEIIIEE"
     gene            complement(3005..3697)
                     /locus_tag="PK087_00855"
     CDS             complement(3005..3697)
                     /locus_tag="PK087_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17848.1"
                     /translation="MHQLLTRLNTKKMCLIASLPENSYELAKIAWEAGVDAIKVHINV
                     SLHRASQNHFGSLEENKAVFKSILADSPVPVGIVAGEQGFAAEAVVDEIVRMGFDFIS
                     LYGHHTPPTLVMRDDITNFLALNFSYSFEEMRIIANSFVADILELSIVAPENYGERLS
                     ARELAKYEFIAHLSNKPTVVPTQKLIYPSDVIALYKTGVKAVMVGAIAMGKNKESIKA
                     TLKAFRSEIDKL"
BASE COUNT         1140 a          861 c          669 g         1111 t
ORIGIN      
        1 gagggggcat aacaaaacga aaagaagcct ttttaaatga gttaaatgtc gccgttaatc
       61 gcattgttga tcccaattat tggggttcca ccgtcttaag catcggtcgc tttatgaatg
      121 atggcggttt aattggggcc ttggtccatc accttaatca aagagaaaca gtccaaaact
      181 aaaaaagcgt taaccaacgc tttttttatg actttttcaa agccactttt ttaataatct
      241 ggccttgctt gactaattgt ttttgtacta aacgataatc caagtccttc ggagtcaagc
      301 ccctttgcac acaaagagct gccaacactc cggctgcttc acctaaagcc ataactgagg
      361 gcgtaacccg taaggaagca ttggcttcat gagttgcgct gacacaacgg ccggtaacca
      421 ctaaattgtt tttacctttc ggaattaaaa cccgaagcgg aatctcatag catttatctt
      481 cactttgttc aaacaactcc aattgttcac tgtgcggtga atgaatatca atcggaaaag
      541 ctgataaaca gatagaatca tcaaattcac gacgatttaa aatatcctca acctgcataa
      601 tgtattcacc gataacatgc ctagtttctc gaacaccaat tcggtagggt gttcttacaa
      661 tcatgctgtt ttcaaaaccg ggaatatact tcttaagaaa agcaaaggct tttttaatct
      721 gtaaacgtcc ttccatttcc gctttggtca gttcaaaggc atcaatgccg gataattgct
      781 gcacccgggt catattaatt gatacttgct tagcttggac atcttgaaac aataaaacgc
      841 ggtcgcgaat taaatcaaac tccccggcat ctttcgcttc ctgaactttc ttaaaaaacc
      901 cagcaatagc caagtaatga taatcataat catctttcat aatgacatca tcggcattgg
      961 cttttaagaa ggcttttaag cggggaagat caacattgtt aaccgaaaaa agcatggaca
     1021 tcggttgtgc taaatggtcg ctttgtcgtc cgaaaacggt ctcgcatccc ataagggcac
     1081 aaacatcacc atccccggtt gcatcaatga cgatggttcc gtataagtct aagaacccgc
     1141 ttttagtggc gaccttaacc cctaaaatcg tattttgttc agaaataaca tcgcacacaa
     1201 aggcatgaag caacaaatca ataccttcct cacgtatgta atcaaaaaag acttctttaa
     1261 tgccttccac atccacgggc gtgatggaat ccgcaaaacc aagcggatca ggaagatgac
     1321 caaaactata gcctttggct tgcaggcgtt ttacaatctc ttcaccaatc cctttaataa
     1381 tctggtcttg gtgattatga aaagtcatcc agggcccaac caaggataaa acactgttcc
     1441 ccccgagtaa gcccgatgtc tccacaagca aaacttgggc atgattcctt ttggcactaa
     1501 tggccgctac aattccgctg ggtcctgccc cgcatacaat tacatcatat ttcatcttat
     1561 tcctcaatta ttatttcggc ttcaaacgtt ctatgccatg gaagagtaat ccgataattg
     1621 gtaattccca gccaatcatc gtattgttgc ataagcatcc ggatttcgga caatacttca
     1681 tcgctgttta ctcctaaatg ataaacatta tagccttttt ttagatattt ttcattaacc
     1741 gtccggcgaa caatatactg atataaacag tcatctaaaa agcgttcgtt acgaaactta
     1801 aagttgtttt tttgcggatt ggcgattttc gccgctattt cacacaaagc cgaccccatg
     1861 gcattagaag cggtattcca agcggaataa acttgcaatt tcttaaggtc aatatctaag
     1921 agcaaatccc atgatccgcc attagggaac aacaaatcaa ccagcgccac tggccggtgg
     1981 gaatgtaaaa actgatttaa ttctttgatg tagcaatgat attgattatc tttcgtcggt
     2041 aaaatcggaa ttgtggaatt cgtgtccaaa ttgatatttc ttgcctccgc atagatggac
     2101 aaaataaagt ccgcttgttc ccaagaatca gtggtcttaa agcccatggt ttccaacatc
     2161 tgcttaagat tttctgaaaa caattgatct tcaaacagca taactttcgc aagaacatcg
     2221 ggatgaggca aatgaacaaa taagctaggg cttatttttt ttatttctaa aataatcttc
     2281 ccgagcaaca ctacgccacc ttcatccgta ccgttataaa atttaacccg cttttctaat
     2341 tgttggtgct taatcatcct ttgtaagact tcttgctcga ttttttgcac accgtagggc
     2401 atggaatctt cctgaagtaa gagcaaataa tcaacaattt gttggttaac taaatccaag
     2461 aaaaacttat tcagttcatg tttaacttgc cgagctttgg tgtaagtcgc aatcatgtcg
     2521 gcgggaattt cttgaagcaa ttcctgtaat tttgcttttt ctttttcatc attaaagaaa
     2581 taagttcttc ccagcaaacg agaatattca ttcattttcc cccaatactt ttccgtttct
     2641 ttgtttaagg ccgtaatgct cgttcgcata atcgtatcaa acacatatat ttttaaatgg
     2701 gggaatcttt ttttatattc cgagaatacc tccaagcggt ccataaccgc gaaagcatca
     2761 atttggccca gcctggcttg caccaaaccg ccactgcaaa gtccgtccgc agcaacaatt
     2821 aaataatccg tatcagtgat ttcgtcatcc agccaggcta acatttggtc aaaattagct
     2881 cccaccaata aatcaccgca gagggcccgc ggatacatcg tcaactcata accggccatt
     2941 ttagctaaat cgtttagcca aacagtatta caaggacgac tatctaaagg aataaccgtt
     3001 attttcacag cttatcaatc tcgcttcgaa aagcctttaa agttgcttta atgctttctt
     3061 tatttttacc cattgcaatg gcgcccacca tgacagcttt cactcccgtt ttatataagg
     3121 caataacatc ggaaggataa ataagctttt gcgtcggcac aaccgtcggt ttattagata
     3181 aatgggcaat aaattcatat ttagctaatt cgcgcgcgga caagcgttcc ccgtaatttt
     3241 caggtgcaac aatcgacaac tccaaaatat ccgctacaaa cgaattggca ataatacgca
     3301 tttcttcaaa gctgtaactg aaattaaggg ccaaaaaatt ggtaatgtca tcacgcatca
     3361 ctaaggttgg gggcgtatga tgaccataga gagaaatgaa atcaaaaccc attctcacaa
     3421 tctcatccac caccgcttcc gctgcaaatc cttgttcacc cgccacaatc ccaaccggaa
     3481 ccggggaatc ggctaaaatg cttttaaaaa cggctttatt ttcttccaaa gagccaaaat
     3541 ggttttggct ggctcgatgt aaagaaacat tgatatgaac tttaatggcg tcaacaccag
     3601 cttcccaagc tattttcgca agctcataac tattttccgg aagactggca atgaggcaca
     3661 tttttttagt attcagtcta gttaataatt ggtgcatatt ctcctctttt gatttggccg
     3721 ataattggtt ctaaattgtc agcgattttc ataaatccca aatcattggg gtgaatgctg
     3781 t
//
LOCUS       DAOSGN010000030         3728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_22793_length_3728_cov_3.125238, whole genome shotgun sequence.
ACCESSION   DAOSGN010000030 DAOSGN010000000
VERSION     DAOSGN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22793_length_3728_cov_3.125238"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PK087_00860"
     CDS             <1..428
                     /locus_tag="PK087_00860"
                     /inference="COORDINATES: protein motif:HMM:NF021196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17849.1"
                     /translation="FHMAVFTKNWSISFYHIQKLSVTKLKQKFNNIDDFLDPNNADMI
                     STRNLVGVFKCFSSQSKNYDYYISIFDTFIEFEKTRNLAAHEIIGYDEENSKKININE
                     AKKLMNKLIELFNYISRDIFNQIDWSFYDKLNEYLISFL"
     gene            454..2754
                     /gene="cas10"
                     /locus_tag="PK087_00865"
     CDS             454..2754
                     /gene="cas10"
                     /locus_tag="PK087_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOH17850.1"
                     /translation="MKENYQSEIMKTTLAALLHDIGKFYQRTGAKINPQSFDSSFTKE
                     FGQYKHAAFTSKFISEFFPTLHPILSLAASHHITNNEISKADQLASAHDRKSDEEEDT
                     EFEHSRYDFIMKRMNNIFNEVILSENPETKKTFLTIKPLTQFELTDKDRIENLEQAKQ
                     EYKVVFNEFTSRCKGIVSPNVFVIHHQLYPLLKTYTGAIPSATYQQNNPSFKPTVSLF
                     DHLKLTAALASVLKASDYQYGKEGQDVKLVFLEYDLSGTQDYIFQVTEGEETKRDIAK
                     SLRTRSFFLLLLTDLIGFKLVHDFSLSYENILFSSGGRGIVLLPFLPDFEDKIQKTIS
                     CIEKEIFEKFKGKISFSFAKNVIDVNEFENNYQNIMNAQKKTVLSGKKQKFKTLFSNP
                     KFSFVSKPLLNACELCESYEAEKNNRCHDCNDFIELNSILVNNKKIFVEYDFSKKNNT
                     SNLSFSFQSLGNINIYVQLPEVNDFSYYVTYNGDELGESKFYANLNAKGKSFNEIADL
                     SEGDKKLGLLKMDVDNLGLIFYKGINHEYKTFSKVLMLSRFMDYFFSKHLFDLMTEKR
                     KNHIYINYSGGDDLVLIAPASEILSIASEINNAFHLFTSQNKDLHISSGIDIFDSKSP
                     IRYAVLQAEAALDQSKKQEHKNSFTIFGTTINNSDMKQVIDECEYYQNKLAKKQISRS
                     IIYKLYSAISNSVDNNNPIEAFSRFIPLLSYSFERNIDDESEKTKLRKLFVRRDINIS
                     LLNLYKVIFEYALLKTRNVKEDKNEQ"
     gene            2744..3193
                     /gene="csm2"
                     /locus_tag="PK087_00870"
     CDS             2744..3193
                     /gene="csm2"
                     /locus_tag="PK087_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOH17851.1"
                     /translation="MNNRNYYGNYRQDNRPESNIKKIINIIKNTKQTLFKDFISEDLY
                     LPEGKAYQIANELTKTKTNQIRKVFSMIKEAEYKAKNKDFESGRDTLYMIVPLMAYSV
                     SRELVDKDLFELIKVCITSEKIKSSKDIETFSKLFESIVAYTKEGRK"
     gene            3195..>3728
                     /gene="csm3"
                     /locus_tag="PK087_00875"
     CDS             3195..>3728
                     /gene="csm3"
                     /locus_tag="PK087_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOH17852.1"
                     /translation="MADIRLTKTAVLEIELHILTGIHIGGSKEIYGIGGIDNPVIKNP
                     LDNKPIIPGSSFKGKIRNLLELNGQEEEVEKLFAGDDLAPSRVIFRDMKLSDDSSDKI
                     QEILGFGVFTEVKAENTIDKRTGKANPRLCERVPSGAVFQSEVVVNAYNDEDVQEIVS
                     LLKQGFQLLELNYLGGSG"
BASE COUNT         1367 a          527 c          633 g         1201 t
ORIGIN      
        1 aatttcatat ggctgttttt actaaaaact ggtcaatttc attttatcat atacaaaaac
       61 tttcggttac aaaattgaaa caaaaattta ataatattga tgatttttta gatcctaata
      121 atgctgatat gatttccaca agaaatttag tcggagtttt taaatgcttt tccagtcaat
      181 caaaaaatta tgactattac atttcgatat ttgatacatt tattgaattt gaaaaaacaa
      241 ggaatttagc agcacatgaa attattggtt atgatgagga aaacagcaaa aaaataaata
      301 ttaatgaagc taaaaaatta atgaataaac taatagaact ttttaattat attagtagag
      361 acatttttaa ccaaattgat tggagctttt atgataaatt aaatgaatac ttgattagtt
      421 ttttataaaa aataataaag aaaggagaaa aaaatgaaag aaaactatca gtcggaaata
      481 atgaaaacaa cattagcggc actgcttcat gacatcggca agttttatca aaggacagga
      541 gctaaaatca acccccaatc ttttgatagt tccttcacca aagagtttgg gcagtacaag
      601 cacgctgctt tcacctcaaa atttatttct gagtttttcc ccactcttca tcccatttta
      661 tctttagccg cttcccacca cattaccaat aatgaaatat ccaaagcgga tcaattagct
      721 tcggctcatg atagaaaaag tgatgaagag gaagacaccg aatttgagca tagtcgttat
      781 gactttatta tgaagcggat gaataatata tttaacgaag ttatattatc agaaaatccg
      841 gaaaccaaaa aaacatttct tactatcaaa cccttaacgc aatttgagtt aacggacaaa
      901 gaccgtattg aaaacttaga gcaagcaaag caagagtaca aagtcgtatt taatgaattt
      961 acctcaagat gtaaaggtat tgtttcaccc aatgttttcg ttattcatca tcaactttac
     1021 cccttattaa aaacttatac cggtgctatt ccgtctgcga cctaccaaca aaataatcct
     1081 tcatttaaac caaccgtcag tttgtttgat cacttgaaat taacggctgc actggcgagt
     1141 gttttaaaag cgagtgatta tcaatatggg aaagaaggac aagatgttaa acttgtcttt
     1201 ctggaatatg atttatcggg aacacaggac tacattttcc aagttaccga aggggaggaa
     1261 acgaaacgcg atattgcgaa atcgttacgg acaagatctt tcttcttatt gttattaact
     1321 gatttaatcg gttttaaatt ggttcatgat ttttcattat cttatgaaaa cattctattt
     1381 tcctcgggag gacgaggaat tgttctctta ccgtttttac ctgattttga agacaagatt
     1441 caaaaaacaa tttcttgtat tgaaaaagag atttttgaaa agtttaaagg taaaatttcg
     1501 ttctcatttg caaaaaatgt gattgatgtt aatgaatttg aaaacaatta tcaaaacatc
     1561 atgaatgctc agaaaaaaac ggttttgagc ggtaaaaaac aaaaatttaa aaccttattt
     1621 tcaaatccta aattttcatt tgtatcaaag ccgcttttaa atgcatgcga actttgtgaa
     1681 agctatgaag cggaaaaaaa caatcgttgc cacgattgta atgactttat tgagttaaac
     1741 agtattttgg ttaacaataa aaagatattt gtggaatatg atttttctaa aaaaaataat
     1801 acttcaaatc tttccttttc ttttcaaagt ttaggaaata ttaacattta cgtgcaatta
     1861 ccggaagtaa atgatttttc ttactatgtt acttataatg gcgatgaact aggagaatcc
     1921 aaattttatg ccaatttaaa tgccaagggt aaaagtttta atgagattgc tgatttgtcc
     1981 gaaggtgata agaaattggg attattaaaa atggacgttg ataatttagg tttaatcttc
     2041 tacaagggca ttaatcacga gtataagact ttttctaaag tcttgatgtt atcaagattt
     2101 atggattatt tcttttctaa acatttgttt gacttaatga cggaaaagag aaaaaatcat
     2161 atttacatta actattccgg tggcgatgat ttagttttga ttgctccggc aagcgaaatt
     2221 ttatcaattg cgagtgaaat taacaatgcc tttcatctct ttacatccca aaacaaggat
     2281 cttcatatta gttccggtat tgatatcttc gattccaaat caccgattcg ttatgctgtt
     2341 ttacaagctg aagcggctct tgatcaatca aaaaaacaag aacacaaaaa tagtttcact
     2401 atttttggga caaccattaa taatagcgat atgaagcaag taattgatga atgcgaatac
     2461 tatcaaaata aattggctaa aaagcaaatt tctcgtagca ttatttataa actttatagt
     2521 gcaatatcga atagtgttga taataacaat cccatcgaag cttttagccg ttttattccg
     2581 ctactctcgt attcttttga acgaaacatt gatgacgaat ctgaaaaaac caaacttaga
     2641 aaattgttcg ttcgaaggga tattaatatt agtttattga atttgtacaa agttatattt
     2701 gaatatgctt tattgaaaac aagaaatgta aaggaggata aaaatgaaca ataggaatta
     2761 ttatggtaat tatcgtcaag ataatcgacc ggaatcgaat atcaagaaga taattaacat
     2821 cattaaaaac actaagcaga cactttttaa ggattttatt agtgaagatt tgtacttgcc
     2881 agaagggaag gcttatcaaa ttgccaatga attaactaaa acaaagacta accaaattag
     2941 aaaagttttt agtatgatta aagaagcgga atataaagcc aaaaacaaag attttgaaag
     3001 tggaagagac acgctttata tgattgttcc cctcatggct tattctgttt cccgcgaatt
     3061 agttgataag gatttgtttg aattaattaa agtttgtatt actagtgaaa aaattaaaag
     3121 ctcaaaagat attgagactt ttagtaaatt gtttgaaagc attgttgctt atacaaaaga
     3181 agggagaaaa taaaatggct gatattagac taacaaaaac tgctgttttg gaaattgaat
     3241 tacacatttt aacaggtatt cacattggtg gctcaaaaga aatttatggc attgggggaa
     3301 ttgataatcc cgttattaaa aatcctttag acaacaaacc cattattcct ggtagtagct
     3361 ttaaaggaaa aattcggaat ttgttggaat taaatggaca agaggaagag gtagaaaaat
     3421 tgtttgcggg tgatgatctt gccccctcaa gagtcatctt tagagacatg aaactttctg
     3481 acgattcaag tgataaaatt caggaaatat taggttttgg cgtgtttaca gaagtgaaag
     3541 cagaaaacac gattgataaa aggacaggaa aagctaatcc aagattgtgc gaacgcgtgc
     3601 cttcaggagc cgtttttcaa agtgaagttg ttgttaatgc ttataatgat gaagatgttc
     3661 aggaaatagt ttctttgttg aaacaagggt tccaactatt ggaattaaat tatttaggcg
     3721 gtagtggc
//
LOCUS       DAOSGN010000031         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_23098_length_3693_cov_3.107752, whole genome shotgun sequence.
ACCESSION   DAOSGN010000031 DAOSGN010000000
VERSION     DAOSGN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23098_length_3693_cov_3.107752"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..611)
                     /gene="rplK"
                     /locus_tag="PK087_00880"
     CDS             complement(183..611)
                     /gene="rplK"
                     /locus_tag="PK087_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOH17853.1"
                     /translation="MAKKKITKVVKLQLAAGKATPAPPVGPSLGQTGINIQQFCLQFN
                     ERTKDMIGNVVPAIISVYEDRSFTFITKTPPASDLLKKAAGIKSGSANALKTKVATIS
                     REKVREIAKTKLDDLNAYTVEAGTKIIEGTARNMGIVVED"
     gene            complement(849..1385)
                     /gene="nusG"
                     /locus_tag="PK087_00885"
     CDS             complement(849..1385)
                     /gene="nusG"
                     /locus_tag="PK087_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOH17854.1"
                     /translation="MNERAWYVVQTYSGCENAAKKNIERRIESMDMGNLVFRVLVPEE
                     KVVEKKKNGELHEVIVKPYPGYVFIDMVVTDESWFMVRNTPMVTGFLGSSGGGAKPVP
                     LTEEEMAPLLKMCGLATEKAFEGKIGDKVIITAGTFAQQEGIIDAIDNQKGIVKVLIY
                     AFGRATPAELSFNEVKIL"
     gene            complement(1393..1830)
                     /gene="secE"
                     /locus_tag="PK087_00890"
     CDS             complement(1393..1830)
                     /gene="secE"
                     /locus_tag="PK087_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOH17855.1"
                     /translation="MPEETKKTNSILAIFTKEYKYEGLLLLFLSIIAIVLGSMVLIGE
                     SSGGTKGLSINENVFLIGDYPKAFAWILIILGVMSLVLAVWPFFRPSIAEVKRVSWPT
                     KGTLFQNTAIVFAFILIMSLFFLLADFLLGYLLQFFNWLAGKM"
     gene            complement(1840..1989)
                     /gene="rpmG"
                     /locus_tag="PK087_00895"
     CDS             complement(1840..1989)
                     /gene="rpmG"
                     /locus_tag="PK087_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOH17856.1"
                     /translation="MAEKVILICEECLSRNYTFKRNKLTNTERMVIKKFCKKCNKHTT
                     HKETK"
     gene            complement(2045..2575)
                     /locus_tag="PK087_00900"
     CDS             complement(2045..2575)
                     /locus_tag="PK087_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOH17857.1"
                     /translation="MYQYNDYELLYLISENDDWALDIMFQKYIPLIKARIRSFKIKYW
                     NYDDFFQEGLIMLHRAIQTFNPESRKTFNKYFDLILQRKFIQILKKESYHFYNVDLVG
                     IGEMLMETPTIPQAIIDYQEIIARCHFSKFEARVLSYLTQGEKISNIATALNVSVKQV
                     YDANDRIRRKIRASNN"
     gene            complement(2580..3293)
                     /gene="rlmB"
                     /locus_tag="PK087_00905"
     CDS             complement(2580..3293)
                     /gene="rlmB"
                     /locus_tag="PK087_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOH17858.1"
                     /translation="MSEVIYGKKVIQTAVTNRFPIANVRLTAENQALIAFLKTHEINY
                     QIVSKDILNRITPHHQGVIAEISGFPNYTLDQLISEKPKGKSLPLIVALDGLEDPHNL
                     GAIIRTVEATSGDGVIFAKNRSVKLNATVAKVSTGAIFNVKCSEVVNLTQTLKYLKTK
                     GYWVVGTDASAKINYSSLKYDFPMILVIGSEGKGISRLVLEQCDYLVSIPMMGVINSL
                     NASVSAGILIYEIRKQQWE"
     gene            complement(3286..3672)
                     /locus_tag="PK087_00910"
     CDS             complement(3286..3672)
                     /locus_tag="PK087_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOH17859.1"
                     /translation="MKPELLDGANLAFIGDAYFELFIREYLINQQITNQNDLHKLAVR
                     YVSASAHHKIVEKLELMLTEEERTIFKRGRNHKYHFSRKNVKMGEYLSSSGLEALIGF
                     LYLKKDFARLSVILNQAIAIIEGSNE"
BASE COUNT         1152 a          759 c          592 g         1190 t
ORIGIN      
        1 gcatccatta aattataacg aacattgcgg tcaactaatt tggcagcttc agcatatttt
       61 cgacttcttt ttgccattct aattttttcc tccttaaatg tggtttagcg gaaattcctt
      121 ccacagttta agttgtaata ttacaacttc tttggtaaat gaattttcgt tgataagatt
      181 ttttagtctt caacaacaat tcccatgttt cttgcagttc cttcaatgat ttttgtgccg
      241 gcttcaaccg tataagcatt taaatcatcc aactttgttt tcgcaatttc gcgaactttt
      301 tcacgagaaa tagtcgcaac tttcgttttc aaagcattag cggagccgct cttaattcca
      361 gccgctttct ttaacaagtc ggaagctggt ggagttttgg tgatgaaagt aaaagaacga
      421 tcctcataaa cagaaatgat ggccggaaca acattgccaa tcatgtcttt ggttctttcg
      481 ttaaactgaa gacagaattg ttgaatatta atacccgttt gtcctaagct gggaccaaca
      541 ggtggtgctg gtgttgcttt tccggcagct agttgcaatt taacgacttt cgtaattttt
      601 ttctttgcca cgagacacac ctcctttttg tccgtgatgt ggttaattgt gagtttttta
      661 actctcccac gaataataaa atagtgattg cgattattgc acgctagagt attctatcat
      721 atttgccctt taatgtcaat cattttaaag cgaattacag cggaggcttt ttccaaagca
      781 aaacatgagc aacaagcaat taaaaaggcc caaagagcca atattagctc cttgaaccat
      841 catctcattt aaagaatttt aacttcatta aaggataact ccgctggagt cgctcgacca
      901 aaagcataaa tcaagacctt cacaatccct ttttggttat cgatggcatc gataatacct
      961 tcttgctggg caaaggtccc agcagtaata atgactttgt caccaatttt tccttcaaaa
     1021 gctttttccg tggcaagacc gcacattttt aaaagcggag ccatttcttc ttccgttaaa
     1081 ggaaccggtt tagcaccacc accggatgaa cctaaaaaac cggtcaccat aggtgtatta
     1141 cgaaccataa accatgattc atcggtaaca accatgtcga taaaaacata acccgggtaa
     1201 ggtttcacaa tcacttcatg aagttcgcca tttttctttt tttccactac tttttcttcg
     1261 ggaacaagga cacgaaaaac gagattcccc atatccatcg attcaatacg acgttcaatg
     1321 ttttttttcg ccgcattctc acaacccgaa taggtttgaa caacatacca agcacgttcg
     1381 ttcataagca ccctacattt tcccggctaa ccaattaaag aactgtagta aatagcccaa
     1441 aagaaaatca gcaagcaaga aaaataacga cataattaaa atgaaagcaa aaacaatggc
     1501 tgtattttgg aacaatgtcc cttttgttgg ccaagaaact ctttttactt ccgcaatcga
     1561 cggacggaag aaaggccata ccgctaaaac taatgacata acaccaagaa taatcaaaat
     1621 ccaagcaaag gccttcggat agtcaccaat caaaaacaca ttttcattaa ttgataaacc
     1681 cttggttcca ccggaacttt caccgattaa aaccatcgat ccaagaacaa tcgcaataat
     1741 ggataagaac aagagaagca aaccttcata tttgtattct tttgtaaaaa tagctaaaat
     1801 actatttgtt ttttttgttt cttctggcat aataccctcc tatttggttt ctttgtgggt
     1861 tgtatgtttg ttgcattttt tgcaaaactt cttaatcacc atgcgttccg tgttagtcaa
     1921 tttattacgt ttaaaggtgt aattgcgaga caaacattct tcacaaatta agattacttt
     1981 ttcagccata cgactcctta aaaatcacta atgtatttta tcaaaatttt ccttggctgt
     2041 caagtcaatt gttactggcg cgaatctttc gacgaatgcg gtcattagca tcatagactt
     2101 gtttaaccga aacatttaaa gccgtggcaa tattactaat tttttcacct tgagttaaat
     2161 aacttaagac gcgggcttca aatttactga aatgacagcg agcaataatt tcttgataat
     2221 caataatcgc ttggggaata gtcggtgttt ccatcagcat ttcgccaatg ccgaccaaat
     2281 caacattgta aaagtgataa ctttcttttt taaggatttg aataaatttc ctttgtaaaa
     2341 ttaagtcaaa atatttgtta aaagtttttc gagattccgg attaaatgtt tgaatggcgc
     2401 gatgcaacat aattaatcct tcttgaaaaa aatcgtcata attccaatat ttaattttaa
     2461 aactacgaat tcgtgctttt attagaggga tgtatttttg aaacatgata tccaatgccc
     2521 aatcatcgtt ttcactgatc aaatataaca attcataatc gttatattga tacatatttt
     2581 tactcccatt gttgttttct tatttcataa attaagattc ccgccgaaac agaagcgttt
     2641 aaggaattaa tcactcccat catcggaatc gagactaaat aatcacattg ttcaagaact
     2701 aaacgcgaaa ttcccttgcc ttcactgcca ataacgagaa tcatcgggaa atcgtatttt
     2761 aaacttgaat aattaatttt tgcagaagca tccgtcccca ccacccaata acctttggtt
     2821 tttaaatatt taagcgtttg ggttaaattg acgacttcac tacacttaac attaaagatt
     2881 gctcccgtag aaactttcgc aaccgtcgca tttaatttca cactgcggtt cttggcaaag
     2941 ataacaccat caccgctagt agcttcaacc gtccgaataa tcgctcccaa attatgtgga
     3001 tcttcaagac catctaaagc aacaattaag gggagggatt ttccttttgg tttttcacta
     3061 attaattgat cgagagtgta attcggaaac ccgctaattt ccgcaatcac cccttggtga
     3121 tggggagtga ttcggttcag aatgtccttg gaaacaattt ggtaatttat ttcgtgggtc
     3181 ttgagaaaag cgattagcgc ttgattttcc gccgttaaac gcacattagc gataggaaaa
     3241 cgatttgtta cggctgtttg gataactttt tttccataaa ttacttcact cattactacc
     3301 ctcaataatc gcaatcgctt gattcaaaat gacggataaa cgcgcaaaat ccttttttaa
     3361 atacaaaaaa ccaattaatg cttccaaacc cgaactgctt aaatactcac ccattttgac
     3421 atttttgcgg ctgaagtgat atttgtgatt acggccacgt ttaaaaatag tccgttcttc
     3481 ttccgtcaac atcaattcca gtttttcaac tattttatgg tgagctgaag cactaacata
     3541 gcgaactgcc agtttatgca aatcattttg attagtaatc tgttgattga ttaaatattc
     3601 gcgaataaat aattcaaaat aagcatcgcc aataaaggcc aagttagcgc cgtctaataa
     3661 ctccggtttc acagaataat gcctttttca tta
//
LOCUS       DAOSGN010000032         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_23154_length_3685_cov_3.739669, whole genome shotgun sequence.
ACCESSION   DAOSGN010000032 DAOSGN010000000
VERSION     DAOSGN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23154_length_3685_cov_3.739669"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..420)
                     /locus_tag="PK087_00915"
     CDS             complement(16..420)
                     /locus_tag="PK087_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17860.1"
                     /translation="MFLSLLSREEKYYFIDLLALIVSVDGNTTELEKQIVNRLKFEIG
                     EDILKYRKSSLSKEKLIEYFAQKPKSTKNIVFMNLISSFLYDEFYCAEEHFLMEEIQN
                     KFEITNKKRAELMKVVYAERDVREKARRVISE"
     gene            complement(472..1227)
                     /locus_tag="PK087_00920"
     CDS             complement(472..1227)
                     /locus_tag="PK087_00920"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOH17861.1"
                     /translation="MMLWTTMEMIHDLLGHDQLKIIQKEGMFAFSLDSMLLADFVVTK
                     NIPQRIIDLGCGNAPIPLYLTLKTTSPIIGVELQSEAFTLAVRSVALNHKQEQITILQ
                     ADIKNVHQQLGANTFDIVTSNPPYFKYHHHSRTNQSDFLTIARHEVKITLADIITEAR
                     KLLKTKGSLYMVHRCERLAEIVSLLEENHFGIKRIRFVYPRVDSASALLFLFEAKQNQ
                     KHDVVVEPPLYVLKTDEQYTDEALAIFNFKKPQ"
     gene            complement(1193..2062)
                     /locus_tag="PK087_00925"
     CDS             complement(1193..2062)
                     /locus_tag="PK087_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOH17862.1"
                     /translation="MIKVVGIGFREVGKIYWFNPFPLHVHLNEKVIVETVRGYELGTV
                     IQEAREIDDAEIEREIKPIVRIATKADILAYEENLEKAKIALETCRHIIKNHQLEMKL
                     LDCEYTIDRQKIIIYYTAEGRVDFRELLKDLASEFRIRIELRQVGPREGAKFIGGMGP
                     CGRAVCCVSHLREFGQVTMKMAKDQGMTLSSSKVAGLCGKLMCCIAYENDLYQELRAR
                     IPLVGDMVKTPTCDGCKVVSVDLLREIVKTANGDNIEVWQAKDLVKLSGHEEDVKPED
                     LTDDVVDNNGNDS"
     gene            complement(2077..3060)
                     /locus_tag="PK087_00930"
     CDS             complement(2077..3060)
                     /locus_tag="PK087_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH17863.1"
                     /translation="MRFAAVLRNQKKVIKLFQNSLKKNRFVHTYLFEGAKGTSKREAA
                     YYFAALMLCTGVSKPCLECENCHKVMKEIHPSVFYVAPDNGMIKKEQAEALEKEFSMT
                     ALTEGKRIYIIDEIDKANASAANSLLKLLEELSGDNYVIMTTENINNVLSTIKSRSQI
                     VSFDRIPVEEVATELINKGIEAETAFVFARESNSASEVLMILQDEKVLPIYELAKKVA
                     VAICNPALHPLLTLINEGDSLVKEPNKRYHQLFVDLLLSVANDKLYYITNQINKIAFR
                     DLMQIISDQVCLKQDKIIKEVETILKFKQRLRYNVNLELFYAQLFIELKQI"
     gene            3217..3546
                     /locus_tag="PK087_00935"
     CDS             3217..3546
                     /locus_tag="PK087_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011328163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOH17864.1"
                     /translation="MGKYCTNCGAELNENQEVCLNCGVIVQRKTDYRDPFAPTTANGK
                     NKYIAGLFGIFLGIFGVHKFYLGQTGTGILYAFFFWTGIPAIIGLVEGILLLAMPDEE
                     FNKRYGH"
BASE COUNT         1141 a          745 c          588 g         1211 t
ORIGIN      
        1 atgatggcac gttcttcatt cagagataac tcgacgcgcc ttttcccgaa catcgcgttc
       61 agcataaaca actttcatta gttcagcgcg ttttttattc gtaatttcaa atttgttttg
      121 aatttcttcc attaaaaaat gttcttccgc acaataaaat tcatcgtaca aaaatgaact
      181 gattaaattc ataaagacaa tgtttttagt tgatttgggt ttctgcgcaa aatactcaat
      241 taatttttct ttggaaagtg aacttttgcg atatttaaga atatcttcgc caatttcaaa
      301 tttgagtcga ttgacaattt gtttttccaa ttccgtggtg ttgccatcaa cactgacaat
      361 taacgctaac aaatcaataa aataatattt ttcttcccgt gataataaac ttaaaaacat
      421 gtaatcaact ccttttctct attataacat atttttttat caatttaata attactgagg
      481 ttttttaaaa ttaaaaatcg ctaaggcttc atccgtatat tgttcatcgg tctttaaaac
      541 atataagggc ggctcaacaa caacatcgtg tttttggttt tgcttggctt caaacaaaaa
      601 taagagcgcc gaagcggaat ccacccgcgg ataaacaaag cgtattcgtt taatcccaaa
      661 atgattttct tcaagtaaag aaacaatttc cgcaagtcgt tcacaacgat gaaccatata
      721 aagcgagccc ttggttttta ataattttct tgcttcggta atgatatccg caagcgtgat
      781 ttttacttca tggcgggcga ttgttaaaaa atcgctttgg ttggtacggc tgtggtgatg
      841 atatttaaaa taggggggat tactggtaac aatatcaaag gtattcgctc ccagctgttg
      901 gtgaacattt ttaatatcag cctgaagaat ggtaatctgc tcttgtttat ggttgagggc
      961 aacactgcga accgctaaag taaaggcttc actttgtaat tccacaccga taatcggtga
     1021 agtggtttta agcgttaaat aaaggggaat cggcgcatta ccacaaccca aatcaataat
     1081 tctttgggga atgtttttgg taacaacaaa atcagctaag agcatggaat ctaatgagaa
     1141 agcaaacatc ccttcttttt gaatgatttt taactggtca tgccccaata aatcatgaat
     1201 catttccatt gttgtccaca acatcatcgg ttaagtcttc cggtttaaca tcttcttcat
     1261 gaccggaaag tttaactaaa tctttggctt gccaaacttc aatattatcg ccgttagcgg
     1321 ttttcacaat ctcccttaac aaatcaacac tgacaacttt gcatccatcg caagttggcg
     1381 tcttgaccat atcaccaacc aaaggaatcc gagctcgcaa ctcttgatat aaatcatttt
     1441 cataagcaat gcaacacatt aatttgccac acaaaccggc gacttttgat gaacttaatg
     1501 tcataccttg gtctttagcc attttcatgg tcacttgccc aaattccctt aaatgggaaa
     1561 cacagcaaac cgcacgaccg caaggcccca taccaccaat aaacttcgcc ccttcacgag
     1621 ggccaacctg acgcagttca atccgaatgc gaaattcact tgctaaatct ttcaacaatt
     1681 cgcgaaagtc aacgcgtcct tccgctgtat aataaataat aattttttgg cggtcaatgg
     1741 tatattcaca atccagcaat ttcatttcta attgatgatt tttaataata tgacgacatg
     1801 tttccaaagc tattttcgct ttttccaagt tttcttcata ggccaaaata tccgccttgg
     1861 tagcaatcct aacaatcggt tttatttctc tttcaatttc cgcatcatcg atttcccgag
     1921 cttcttggat aactgtcccc agttcatacc cccgaaccgt ctccacaata actttttcgt
     1981 taagatggac atgcaaggga aagggattaa accaatatat ttttccgact tctcgaaagc
     2041 cgattccaac aactttaatc ataatttctt ctctttctaa atctgtttta attcaatgaa
     2101 caactgcgca taaaaaagtt ccaagttgac attgtaacgc aatcgctgtt taaattttaa
     2161 aatcgtttcg acctctttaa ttattttgtc ttgctttaaa caaacttgat cactaataat
     2221 ttgcattaaa tcacgaaacg ctattttgtt aatttgatta gtaatataat ataatttatc
     2281 attggccacg gacaataata agtcaacaaa caattgatga tagcgtttat ttggttcttt
     2341 cactaaacta tcaccttcat taattagggt taataaggga tgcaaagcgg ggttacagat
     2401 ggcaacggcg acttttttgg ctaactcata aatcggaaga actttttcat cttgcaagat
     2461 catcaacact tcagaagcac tattagattc ccgagcaaaa acaaaagccg tttccgcttc
     2521 aatgcccttg ttaattaatt ctgtcgccac ttcttccacc ggaatccggt caaaagaaac
     2581 aatttgcgac cgcgatttaa tcgttgataa cacattgtta atattttctg tggtcataat
     2641 aacatagttg tcgccactta gttcttctaa taactttaaa agggagttag cggcactggc
     2701 attggcttta tcaatttcat caataatata aatccgcttt ccttccgtta aagctgtcat
     2761 cgaaaactct ttttctaggg cttccgcttg ttctttttta atcatcccat tatctggagc
     2821 aacataaaaa acgctcggat gaatttcttt catcacctta tgacagtttt cgcactctaa
     2881 acaaggcttt gagaccccgg tacacagcat taaagccgca aagtagtacg cggcttcccg
     2941 tttcgatgtc cccttagcac cctcaaataa ataagtgtgc acaaagcgat tcttttttaa
     3001 actgttttga aaaagtttga taactttttt ttgatttctt aatactgcag caaaacgcac
     3061 ccttattttc tccttaatta tcataattat tgtaacaaaa ataacgacaa aaaacaataa
     3121 aaacccacta attgtaatgt ttttgtaagt ttaaggtaat tttttatatt tttaaaaaat
     3181 atatgttata atatcttatg aagaaaggaa ggtcatatgg gaaaatattg tacaaactgc
     3241 ggagcagagc ttaatgaaaa ccaagaagta tgtttaaact gcggtgtgat agttcaaaga
     3301 aaaaccgatt atcgcgatcc ttttgcaccg acaaccgcca acggaaaaaa caaatatatt
     3361 gccggcttgt ttggtatttt cttaggtatc tttggcgttc acaaatttta tttaggtcaa
     3421 accggaaccg gtattcttta tgcctttttc ttttggacgg gcatcccggc cattatcggc
     3481 ttggttgaag gcattttact gttggctatg cccgatgaag aattcaacaa acgttacgga
     3541 cattaaaaaa cagaccccac ggtctgtttt ttgttggaaa caacaaacta tgtttgcact
     3601 tgaatgagca taaaactgat atgatatctc taaattaagt aattaaagtg agctttgatt
     3661 atccttattt tgcttccttt gtgac
//
LOCUS       DAOSGN010000033         3636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_23590_length_3636_cov_3.506562, whole genome shotgun sequence.
ACCESSION   DAOSGN010000033 DAOSGN010000000
VERSION     DAOSGN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23590_length_3636_cov_3.506562"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PK087_00940"
     CDS             <1..195
                     /locus_tag="PK087_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--acyl-ACP N-acyltransferase OlsB"
                     /protein_id="HOH17865.1"
                     /translation="ILPLEEVDEGLAKRQLPSLVKGYLRIGAKFGPDIFFDYPFNSID
                     VLVIMDVDEFDPKWLKKIVG"
     gene            199..726
                     /locus_tag="PK087_00945"
     CDS             199..726
                     /locus_tag="PK087_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH17866.1"
                     /translation="MKNDNELVNELYTHNEQAFQELYQKYYWLVFGVVNKKVKNKEVS
                     SEITQDVFLKMWNNISQYQNNSNFRAWLVTIAKNLAIDYVKAQSARQLNLIINNDVVN
                     NIKQFDEKIHMEYQMDLKKILNELEYKVIGLTKIYHLKRREVAEVLNKPLGTILRVHN
                     EASKKINRFFTNKGE"
     gene            914..1828
                     /locus_tag="PK087_00950"
     CDS             914..1828
                     /locus_tag="PK087_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH17867.1"
                     /translation="MKNKNVALILAMVATLIGASANALSKIGLEGFSPLPMALLRHFF
                     TALFFFTLAFFSKMRLPKLKDVPWFFLTGFSGFAMFSILFNIGLETTDVSTASIIIGT
                     FPIMTVILAAIVFKDKIALGSYVAVGIQFIGIIVLFANKGSFSISVGALLLFLSALML
                     AVYNVTQKHLLKKYQPIEIITYSIIACLLLMSMYIPKAISEIRLASARALWAVIILGV
                     VSSGVSTILWSTAVSKATSVTTITNFAFLTPFLAILYGLILVPEPLALKTIIGGIVII
                     GGSVLFQYFNNRQATKVATEESDAQKTL"
     gene            1809..2552
                     /locus_tag="PK087_00955"
     CDS             1809..2552
                     /locus_tag="PK087_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17868.1"
                     /translation="MPKKLFKIQLILSITYLLLTVFFLLAFHNAMLESFLPTWLSDLL
                     MLFGAVYFGGGAIIIPVAWLILLVYHYQMRDTMDKPAFPSPTKDFLFKQFFPLMSLSF
                     YAINLVYLLFVNQVSSIDFVHFLAFSVLVFSLYVQDHSFNKANWTAFFGLGCGVLVLW
                     FVEMVVGAEFKQAMVEQGELIYGMSYQMMFYLIWILVVFVLAYALIGYFYISNRTKYR
                     HLLMLIVSLFIIFLNIVRQLDVFNRLMLG"
     gene            2561..3496
                     /locus_tag="PK087_00960"
     CDS             2561..3496
                     /locus_tag="PK087_00960"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH17869.1"
                     /translation="MSKFLVTGGAGFIGSEICQQLIALGHEVVALDNLSSGHLENLDS
                     VIDNPRFKFVLGDITDYETCQKACRQRDYVIHQAALVSVPLSIEKPQLNNEINIKGFV
                     NILEASRINQVKKVVYASSSAVYGDNTDLIKLETQTGNVLSPYALSKMADECYAKLYT
                     NLYQLPTVGLRYFNVYGPKQDPSSVYSGVISIFFKKALSAQDLLIYGDGSITRDFINV
                     SDVAHANVLACLTCQNDGEVYNIGTGRAVSIKELAQLVLRITQAKVQIKYATGRTGDI
                     LYSCANVQKAKNELRFLATISLEDGLSQMAACLKP"
BASE COUNT         1025 a          623 c          711 g         1277 t
ORIGIN      
        1 attttaccgc ttgaagaagt cgatgaaggc ttagccaaac gtcaattgcc atcgcttgtt
       61 aagggctatc tacgaatcgg ggcgaaattt ggacctgaca tcttttttga ttatcccttt
      121 aacagcattg atgttttggt tatcatggat gttgatgaat ttgatccgaa atggttaaaa
      181 aaaatagtgg ggtaatttgt gaagaacgat aatgaattgg ttaatgaact ttacactcac
      241 aatgaacaag ccttccaaga actatatcaa aaatattatt ggttagtttt tggagttgtt
      301 aacaagaaag taaaaaacaa agaagtttct tctgaaatca cgcaggatgt ttttttgaaa
      361 atgtggaaca acatttccca atatcaaaac aacagcaatt ttcgggcttg gctagttacc
      421 attgctaaga atttggcaat tgattatgtt aaggcgcaat ccgcccgcca gttgaatttg
      481 attattaata atgatgtagt taataatata aagcaatttg atgaaaagat tcatatggaa
      541 tatcaaatgg atttaaaaaa gattctcaat gaattggaat ataaagttat cggattgacc
      601 aagatttatc atttgaaaag acgcgaggtt gcggaagtct taaacaaacc cttaggcacg
      661 attcttcggg ttcataacga agcctctaaa aagattaatc gtttttttac caacaaaggt
      721 gaataaacta ttattgctct ttctttaatc caaaacttgc ttaatgggaa aatgagccaa
      781 gttgctattg ttttattctt ttggtaaaaa caaaggctta catcacttaa aaacatattg
      841 gttttcaata tgttttttct tgtggaaagg gcggtattaa ggtataatac ctttgatatg
      901 aataggaaca aagatgaaaa ataaaaacgt tgctttaatt ttagcgatgg tagcaacctt
      961 aattggggcc tctgccaacg ctctttcgaa aatcggtttg gagggttttt ctcctcttcc
     1021 catggcctta cttcgccact tttttacagc tctcttcttt tttacattag cgtttttttc
     1081 caaaatgcgt ttaccgaaat taaaggatgt gccgtggttt tttctaaccg gtttttccgg
     1141 ttttgccatg ttttcgatat tatttaatat tggcttggaa acaacggatg tttcaacagc
     1201 cagtatcatt ataggaactt ttccgattat gaccgttatc ttagccgcca ttgtttttaa
     1261 agacaaaatt gctttgggta gttatgtggc ggtaggaatc caatttatcg gcattattgt
     1321 tttattcgct aataagggct cgttttctat ttctgtaggg gctttgttac tctttttgtc
     1381 tgctttaatg ctagccgttt acaatgttac ccaaaagcac ttattaaaaa agtaccaacc
     1441 gatagaaatt attacttata gcattattgc ctgtttgctc ttaatgtcca tgtatattcc
     1501 taaggcgatt tcggaaataa ggcttgcgtc tgctcgagca ctgtgggcgg tcattattct
     1561 tggcgttgtg tctagcggtg tgtccaccat cctgtggtca acggcggttt ctaaagccac
     1621 atcggtaaca acgattacca actttgcttt tttgacgcct tttttagcaa tcttatacgg
     1681 cttgatttta gttcctgagc ccctagcttt aaagacgatt attggtggaa tagtgattat
     1741 tggcggctct gttttattcc aatactttaa taaccgacaa gcaactaaag tcgccacgga
     1801 ggaatccgat gcccaaaaaa ctctttaaaa ttcaactaat tttatcaatt acttatttgt
     1861 tgttgacggt attttttcta cttgcttttc ataacgccat gttggaaagt tttttgccga
     1921 cttggcttag tgatttatta atgctttttg gggctgttta ttttgggggt ggtgccatca
     1981 tcattcccgt ggcgtggctt attttgttag tttatcatta ccaaatgcga gacaccatgg
     2041 ataaaccggc ctttccttca ccaacaaaag attttttgtt taaacaattc tttccgctaa
     2101 tgtctttaag tttttatgcc attaacttag tttatttgct gtttgttaat caggtatcat
     2161 caattgattt tgttcatttt ttggcatttt cagtgcttgt ttttagccta tatgttcaag
     2221 accatagttt taataaagcc aactggaccg ccttttttgg cttaggatgt ggtgttttag
     2281 tgctgtggtt tgtggagatg gttgttgggg cggaatttaa gcaagcgatg gtggaacaag
     2341 gggaattgat ttacggcatg agttatcaaa tgatgtttta tttaatttgg attcttgttg
     2401 tttttgtgct tgcttatgcc ctaatcggct acttttatat ttccaaccgg actaaatatc
     2461 gccatttatt aatgttaatt gtttcacttt ttattatctt tttaaatatc gttcgccaat
     2521 tagatgtctt taatcgtcta atgcttggct aaggaggatt atgagtaaat ttttagtaac
     2581 cggtggagcc ggttttatcg gtagtgaaat ctgccaacaa ttaattgcct tgggtcatga
     2641 agtggttgcc ttggataatc tttccagcgg gcacttagaa aacctcgatt cggtaattga
     2701 taacccgcgc tttaagtttg tgcttggcga tattacggat tatgaaacct gccaaaaagc
     2761 ttgccgacaa agggattatg ttattcatca agccgcactt gtttccgttc ctctttccat
     2821 tgaaaaaccc caacttaata acgaaatcaa tattaaaggt tttgttaaca tattggaagc
     2881 gagccgcatc aaccaagtca aaaaagtggt ttatgcgtca tcttccgccg tttatggtga
     2941 taataccgac ctaattaaac tagaaacgca aaccgggaat gtgctttcgc cctatgcttt
     3001 aagtaaaatg gcggatgaat gctatgctaa actatatacg aacttgtatc aattgcccac
     3061 cgtcggcctt cgctatttca atgtttacgg accgaagcaa gatccgagca gtgtttattc
     3121 cggagttatt tcaattttct ttaagaaagc tttatccgct caagatttgc ttatttatgg
     3181 tgatggttcc attacccgtg attttatcaa tgtttccgat gttgcccatg ccaatgtttt
     3241 agcttgttta acttgccaaa atgacggtga agtttataat attggaactg gacgagcagt
     3301 ttccatcaag gaactagccc aattggtttt aagaatcacc caagcaaagg tccaaattaa
     3361 gtatgcgacg ggccgaaccg gggatatttt gtatagttgt gccaatgttc aaaaagctaa
     3421 aaacgagctt cgttttttag caaccatctc tttagaagat ggtttaagcc aaatggcggc
     3481 ttgtttaaaa ccataaaact tgcttatttt agtccggata atccggactt ttttatttac
     3541 ttttttgttg tatttaacct agaagtggcc ttgccaagag aaaaatgaat attgtaaaat
     3601 gaaaatagaa aaaagattta aagaacaaag ggggtt
//
LOCUS       DAOSGN010000034         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_23638_length_3631_cov_2.712528, whole genome shotgun sequence.
ACCESSION   DAOSGN010000034 DAOSGN010000000
VERSION     DAOSGN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23638_length_3631_cov_2.712528"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK087_00965"
     CDS             complement(<1..411)
                     /locus_tag="PK087_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004024345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOH17870.1"
                     /translation="MNSKNTKKKNLSTLIEKFVDEKIIEDSLEEIVSQRYARYSKYII
                     QDRALPDARDGLKPVQRRILFAMFKLGMFANKPYKKSARIVGDVIGKYHPHGDSSVYE
                     AMVRLSQDFKMLLPLIDMHGNNGSIDGDPAAAMRY"
     gene            complement(404..2347)
                     /gene="parE"
                     /locus_tag="PK087_00970"
     CDS             complement(404..2347)
                     /gene="parE"
                     /locus_tag="PK087_00970"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOH17871.1"
                     /translation="MAKIVNNVYDESKIQVMQGLEAIRKRPGMYVGSTDSRGLHHLIW
                     EIVDNSIDEVLSGNGNYIKIIIHKGNSVEVIDNGRGIPIGMHKTGVPTPQVVFCTLHA
                     GGKFDSTGAYKVSGGLHGVGSSVVNALSEWVEVTIAREGKIFFQRYENGGKKINKSKI
                     IGESKKTGTTVHFKPDPTIFSTTIIDYKQCVQRFKESAYLIKGLKIELFDERTGASNT
                     FFYHEGIAAFVKNINQDKTTLHEPIYLEGTSMDIQVEVAMQYCAKTFTENISSFVNNI
                     RTRDGGTHETGFRTALTKAFNEYARMKGILKDKDPNLDGADIREGLTAVLSVRIGENI
                     LQFEGQTKNKLGTADAKTAVENIVSEKLKFYLNEKGEIANLLIQNALRAFRAREAARK
                     ARDEVRMIRQKITKTANLTGKLAPVQEKNPQTNELFIVEGDSAGGSAKQGRDRRFQAI
                     LPLRGKVINSEKTKTDELLKNEEIMSIIYSIGAGFGMDFNASKSNYSKIIIMTDADTD
                     GAHIQILLLTFFFRYMRELIERGQIYIALPPLYKITNKNNIDYAWTDEELREKAANLK
                     NIIIQRFKGLGEMNPEQLWETTMNPMTRTLIKVNIEDFTDADDRIATLMGDDVESRRE
                     WIENNVSFEYEDNFTLEEVESDE"
     gene            2602..3243
                     /gene="plsY"
                     /locus_tag="PK087_00975"
     CDS             2602..3243
                     /gene="plsY"
                     /locus_tag="PK087_00975"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOH17872.1"
                     /translation="MMEFIKYGLLVIAYLLGSIPFGYLIGKLRGIDIREYGSKNIGAT
                     NIGRVLGFKCGLVAFSLDLLKGAVIVFLFRFGIIPIEYCFLSPMTYGLAAILGHSFSV
                     FMSFKGGKAVATTGGVLLGFAPGLFLFGLVVFIILFFSTKLVSLSSLISSSLTFIGSI
                     GLFLLGYDPLFNLPVDWFFPLCTGIIYLIIVIRHNENIKRLVKHQEQPFHFKK"
     gene            complement(3262..3495)
                     /locus_tag="PK087_00980"
     CDS             complement(3262..3495)
                     /locus_tag="PK087_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HOH17873.1"
                     /translation="MVNLAWWAFTLILLGVLIVGLIAGFFISRTVFTKYLEKNPPVNE
                     KMIRAMMQQMGRTPSEKQVRQVMASMNQAKTSK"
BASE COUNT         1064 a          695 c          602 g         1270 t
ORIGIN      
        1 gtaacgcatg gcagcggcag gatcaccatc aatgctccca ttatttccat gcatatcaat
       61 taatggtaag agcattttaa aatcttgcga taagcgcacc attgcctcat aaactgaact
      121 gtcgccatga ggatgatatt taccaatgac atcaccgaca atacgcgctg atttcttata
      181 aggtttattg gcaaacattc ctaatttaaa catcgcaaac aaaatacgac gttgaacagg
      241 ttttaagcca tcacgagcat cggggagagc gcgatcttga ataatatatt tggaatagcg
      301 cgcataccgt tgactaacga tttcctccaa agaatcttca atgatttttt catcgacgaa
      361 tttttcaata agagtgctta aattcttctt tttcgtattt ttactattca tcgctttcaa
      421 cctcttctaa agtaaaatta tcttcatatt caaaagaaac attgttttca atccattctc
      481 gtcgcgactc aacatcatcg cccattaatg tggcaattcg atcatcagca tcggtaaaat
      541 cttcaatatt gactttgatc aatgttcgag tcatcggatt catcgtcgtt tcccatagtt
      601 gttcgggatt catttcaccc aaacctttaa agcgttgaat tataatattc ttgagattgg
      661 cagctttttc tctaagttcc tcatccgtcc acgcataatc gatattattt ttattagtta
      721 ttttataaag gggcggaaga gcaatataaa tttgtccacg ctcaattaat tcacgcatat
      781 agcgaaagaa aaaggtcaat aacaaaattt gaatatgagc tccatcggta tcagcatcgg
      841 tcatgataat gattttacta taatttgatt tactagcatt aaaatccatc ccaaaaccag
      901 cgccaatgga ataaatgatg gacattattt cttcgttttt taacaattca tcggtctttg
      961 ttttttcaga attgataact tttccgcgta aaggtagaat tgcttgaaat cgacggtccc
     1021 ggccttgttt ggcgctacct cctgctgaat ccccctccac aataaataat tcatttgttt
     1081 ggggattttt ttcttgaact ggagctaatt ttcccgtaag gttagcagtt ttggtgattt
     1141 tttgtctaat cattcgcact tcatctcggg ctttacgagc agcttcacga gcccgaaaag
     1201 ctctcaaagc attttgaatt aagaggttgg caatttcacc tttttcattt aaataaaatt
     1261 ttaacttttc gctaacaata ttttccacag ccgttttggc atctgcggtt cctaatttat
     1321 ttttggtttg tccctcaaac tgaagaatat tctcaccaat gcgtacagat aaaaccgcag
     1381 tcaaaccttc acggatatcc gcaccatcta aattgggatc cttatctttt aatattcctt
     1441 tcatgcgcgc atattcatta aaagctttgg ttaaagcagt gcggaatccg gtttcatgag
     1501 ttccgccatc gcgagtgcgg atattattaa caaaggaaga gatattttcg gtaaaagttt
     1561 tagcacaata ttgcatcgcc acttcgactt gaatatccat ggatgtgcct tcaagataaa
     1621 tcggttcatg gagagtcgtt ttatcttggt tgatgttttt cacaaacgcc gcaattcctt
     1681 catgataaaa gaaagtattg gaagcaccgg tccgttcatc aaatagctcg attttcaacc
     1741 ctttaattaa ataagcagat tctttgaatc gttgcacaca ttgtttgtaa tcaataatcg
     1801 tggttgaaaa aatggttgga tccggtttaa aatgaacggt tgttccggtt ttcttgcttt
     1861 caccaatgat ctttgatttg ttaattttct taccaccatt ttcatatctt tgaaaaaaga
     1921 tttttccttc ccgagcaatc gttacttcaa cccactcact tagagcattg acaacactgg
     1981 aaccaacgcc gtgtaaacca ccagaaactt tataagcacc agtggaatcg aattttcccc
     2041 cggcatgaag agtgcaaaaa acaacttggg gtgtaggaac acctgtttta tgcatcccaa
     2101 taggaatacc acggccattg tcaataactt cgaccgaatt acctttatga atgatgattt
     2161 taatataatt gccatttcca cttaaaactt cgtcaatgga attatcgaca atttcccaaa
     2221 tcaaatgatg aagcccgcgg gagtccgtac tcccgacata catgccggga cgcttacgga
     2281 tggcttcaag tccttgcatt acttgaattt tcgactcatc ataaacatta ttaactatct
     2341 tagccatttt tgatattcct ttcatattga taatttatta tagcatatat ttaataaaaa
     2401 gtaaatgctt acttttttta tttttttata ttcatttatt atatttgagt gcttttaata
     2461 caagtaaaag ctcacttttt gaaaaaacat gactatattt aaaaatgaca aatcaggttt
     2521 ttttgttatt aaatctttac aaaaaactta aaaattaggt ataataataa tagttttatt
     2581 tattagcagt cggaggcacc tatgatggag tttattaaat atggcttatt agttattgca
     2641 taccttcttg gttccattcc ttttggttat ttaatcggta aattacgggg catcgatatt
     2701 cgtgagtatg gaagtaaaaa tatcggcgct actaacattg gtcgtgtttt aggctttaaa
     2761 tgcggtttag tggctttttc tctcgattta ttaaaaggag cagttattgt gtttcttttt
     2821 cgttttggca ttatcccgat agaatattgt tttctttccc caatgactta tggtttggcc
     2881 gccatcctcg ggcatagttt ttccgttttt atgagtttta aaggcggaaa agcagtcgct
     2941 accacggggg gagtcttatt gggttttgct cctggactct ttctttttgg tttagttgtt
     3001 tttattatct tatttttttc aacaaaacta gtttcactca gctctttaat cagttcaagt
     3061 ttaactttta tcggatctat aggtttattt ttattaggtt atgatccgct ttttaatctt
     3121 ccggttgact ggttctttcc tttgtgtact ggaataatct atctcattat tgttattaga
     3181 cacaacgaaa acattaaacg actagttaaa caccaggaac aaccatttca ttttaaaaaa
     3241 taaaagacgc cgcttggcgt cttacttgct tgttttggct tgattcatgg aagccattac
     3301 ttggcgaact tgtttttccg aaggggttct gcccatttgt tgcatcattg cccgaatcat
     3361 tttttcatta actgggggat ttttttccaa gtacttagta aacacagttc gtgaaataaa
     3421 aaaaccggca attaatccaa cgattaatac tcctaaaaga attaaggtga aagcccacca
     3481 cgctaaattg accattcgtt tcactccttt tttgtgatta ttattttact acaaaacaga
     3541 gaaaaaaaca atgaaaatgt ttttagtaaa atattgggca tcttccaagc ctaaatgcga
     3601 caaataaaca attgaaaaag ttcatagaaa t
//
LOCUS       DAOSGN010000035         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_24117_length_3582_cov_3.501559, whole genome shotgun sequence.
ACCESSION   DAOSGN010000035 DAOSGN010000000
VERSION     DAOSGN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24117_length_3582_cov_3.501559"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="PK087_00985"
     CDS             <1..1446
                     /locus_tag="PK087_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17874.1"
                     /translation="VDYLITLQTKVTDDFILNVQNLVESNGPRFLTADEIVVVKDEFV
                     DLFMDNLPSVDDLKLVYELLATGYGQFLEDNDLTALLSDLSTAFATSTNLSIKFSLKM
                     LDAFDKAFFEEVLALVNGEDSEQIVQSEIIILVITYLKNFKDANQALIDEIEAVFTDE
                     QKESLYQGYLQTMLKIMETSGGELPFSLAGMQLSYALIEGSFAVFEDMMDKALTKFVT
                     TDGELLRKIVISDSFVYDWDWENDVEYYYNEATGETYDNWDDFYNAQNEAELAVIKEA
                     LTYYAPTLGTLSNAQITTLIDMIVASVPVEAIATELDMTADEAQALVDLAEGLLGDVL
                     PNLHTLVKNYMDYVVTNDLITKIKALEETIASYEGEDFEEYDHNMTIIFISKHLDAFL
                     TSANQSLVRGIISDVATFVKDESIYPLLGASSVADVTEMETMVNDTFDELVTLVSVIK
                     DYNAETLTLEQEEKIIDFSSLVEFLFGGPAE"
     gene            1554..2261
                     /locus_tag="PK087_00990"
     CDS             1554..2261
                     /locus_tag="PK087_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOH17875.1"
                     /translation="MRIPTTTFTKLPNHVAIIMDGNGTWAKKRGLPRTMGHRQGAKTL
                     LEVVKASQSIGIKYLTVFAFSTENWSRPQEEVDYLMKLPFEFLAQYQDDFQKSEIKFQ
                     AIGKIDDLPEPLQAKIKEVEQQSLNNTGLNLIVAVNYGGHEEIVHACNQLLKQNKNMI
                     TEADFVAQLYTNNIPDVDLLIRTSGQMRISNFLLWQIAYSEIYFTEVLWPDFHKKELI
                     KALKAYESRDRRFGGIK"
     gene            2258..3205
                     /locus_tag="PK087_00995"
     CDS             2258..3205
                     /locus_tag="PK087_00995"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOH17876.1"
                     /translation="MKKRIITGAILMAILIPLIVIDHQIYPLVGILFLIVGIFLSMVA
                     AYEMMNMFYQKYPSLKLLRYLVPIFSGILVYTVYLSTTKGLDINDISAASFVYHFLVV
                     VMLMIFIIISLGIIIFTKSSTAYDMMAVIMTLVYCGLVMGYVISIRYIEPFPFREGSI
                     NSIFRGGRSFAYLYSIVLGTDTFAYLFGIKFGKHKLCAEISPKKTTEGAVAGLVFGSL
                     FGVASVFIFKIGNPANTAETFIIIGGSLVISMIISCSVQIGDLVASKIKRTYEIKDFG
                     KVFPGHGGVLDRFDSLIYAGAVFYIIVQLMQLFVIGVVE"
     gene            3202..>3582
                     /locus_tag="PK087_01000"
     CDS             3202..>3582
                     /locus_tag="PK087_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOH17877.1"
                     /translation="MKKLALLGATGSIGTQVLEIVKNNPDYRIKSIAFGNNVVLGRKI
                     IEEFQPEFVAVRNDTIKEDIFRQFPQLKIGVGEAGLIEAATFVAPGDYLINALVGVLG
                     LVPTVAAIEKGINILLANKETLVVG"
BASE COUNT         1047 a          586 c          772 g         1177 t
ORIGIN      
        1 gttgattatt taattacttt acaaacaaaa gttaccgatg attttattct caatgttcaa
       61 aatcttgtgg aatcaaatgg cccaaggttt ttaacagctg atgaaatcgt ggttgtaaaa
      121 gatgaattcg ttgatttgtt catggataac ttaccatccg tggatgattt aaaattggtt
      181 tatgaattac tggccaccgg ctatggccaa ttcttagagg acaacgactt aacagcttta
      241 ttgtccgatt tgtcgacggc ttttgctaca tcaacaaatt tgtcgattaa gttctcactg
      301 aaaatgcttg atgcctttga taaagcgttc tttgaggaag ttttagcctt agttaatggt
      361 gaagattctg agcaaattgt ccaaagcgaa attattattt tagtaattac ctatttaaag
      421 aattttaaag acgctaacca agcgctcatt gatgaaatcg aagctgtttt caccgatgaa
      481 caaaaggaaa gtttgtacca aggctatttg caaaccatgc ttaaaattat ggaaactagc
      541 ggtggagaat taccgttctc acttgcgggt atgcaattaa gctatgcttt aattgaaggt
      601 tctttcgctg tctttgaaga tatgatggat aaagccctga ctaaatttgt caccactgat
      661 ggtgagttac tccgcaaaat tgttatttcg gattcctttg tttatgattg ggattgggag
      721 aatgatgttg agtattacta taatgaggct acgggcgaaa cttatgacaa ttgggatgat
      781 ttttacaacg ctcaaaatga ggcggagtta gcagtcatta aagaagcgct cacctattat
      841 gctccaactt taggaacctt aagcaatgca caaataacga cgcttatcga tatgattgtt
      901 gccagtgttc cggtggaagc gattgcgacc gaattggaca tgactgcgga tgaagcacaa
      961 gcgcttgttg atcttgcgga agggctattg ggtgatgtgc ttccgaatct tcatacttta
     1021 gttaagaatt atatggatta tgttgttacg aatgacctta ttactaaaat taaagctttg
     1081 gaagaaacga ttgccagtta tgaaggtgaa gactttgaag aatatgatca caatatgacc
     1141 atcatcttta tttccaagca tttagatgct ttcttaacaa gtgccaatca aagccttgtt
     1201 cgtggtatca ttagcgatgt cgccaccttt gtcaaggacg aatccattta tcctttgctt
     1261 ggagcaagtt ccgtcgctga tgttacggag atggaaacga tggtaaatga tacttttgat
     1321 gaactcgtta ctttagtgtc ggtaattaag gattacaatg ctgaaacttt aacgctcgaa
     1381 caagaggaaa aaattattga ttttagttcg cttgtggaat ttttgtttgg gggacctgct
     1441 gaataaactc tttagaccga aaggcgactt tcggtctttt tttatggaaa aatatatatg
     1501 ctttgtgtgg aaaaaaaatc aaaattgtag tataatacct taatggtgaa attatgagaa
     1561 tcccaacaac aacatttaca aaactgccaa atcatgtggc cattatcatg gacggcaatg
     1621 gcacctgggc caaaaaacgg ggtttaccgc ggacgatggg acatcgtcaa ggtgctaaaa
     1681 ccttattgga agtagttaaa gccagccaaa gcattggtat taagtattta actgtttttg
     1741 ctttcagtac ggaaaattgg tcccgcccgc aagaagaagt ggattatttg atgaagctgc
     1801 cctttgagtt tttagctcag tatcaagacg attttcaaaa aagtgagatt aaatttcaag
     1861 cgatcggtaa aatagatgat ttaccggaac ccctacaagc aaaaattaag gaagtcgaac
     1921 aacaaagcct taacaacacc ggtttaaacc tcattgttgc cgttaattat ggtggacatg
     1981 aagaaatcgt tcacgcttgc aatcagttgc ttaagcaaaa taaaaacatg attacagaag
     2041 cggactttgt tgcgcaatta tataccaaca acatccccga tgttgattta ttaattagaa
     2101 cgagcgggca gatgcgcatc agcaattttt tgctctggca gattgcttat agcgaaatat
     2161 attttaccga agtattgtgg ccggactttc acaaaaaaga attgattaaa gctctcaaag
     2221 catacgaaag tcgggatcga cgttttggag gcatcaagtg aaaaaacgga ttattaccgg
     2281 agccatttta atggcgattc ttattccttt aattgtaatt gatcatcaaa tttatccgct
     2341 ggtcggcatt ctctttttga tagtggggat ttttttaagc atggttgctg cctatgaaat
     2401 gatgaatatg ttttaccaaa aatatccatc tttaaaattg ttgcggtatt tagtacccat
     2461 cttttccgga atcctcgttt atacagttta tttatcaacc acgaagggat tggatatcaa
     2521 tgatatttcg gcggccagct ttgtttatca ctttttagtg gtagtaatgt taatgatttt
     2581 catcattatt tccctaggga ttattatctt taccaaatcg tcaacggctt atgatatgat
     2641 ggctgtgatt atgacgcttg tttattgcgg tttggtcatg ggttatgtta tcagtatccg
     2701 ttatattgaa ccttttccgt ttcgtgaagg ttcgattaat tcaatttttc gtggtggtcg
     2761 aagttttgcc tatttatatt ccattgtttt aggaaccgac accttcgctt atttattcgg
     2821 tataaaattc ggaaaacata aattatgtgc ggaaatcagc cctaaaaaaa ctacggaagg
     2881 ggctgtggcg ggcttggttt ttggcagttt gtttggggtt gcttctgttt tcatctttaa
     2941 aatcggcaat cctgccaata cagcggaaac ctttatcatc attggtgggt ccttagttat
     3001 ctccatgatt atttcttgtt ccgttcaaat tggggatttg gtggcttcga aaattaaacg
     3061 aacttatgaa attaaagatt ttggaaaagt ttttccgggt catggtggtg ttttggaccg
     3121 ctttgatagt ttaatttatg ccggggccgt cttttacatc attgttcaac tgatgcaatt
     3181 atttgttatc ggagttgtgg aatgaaaaag ctagctttat taggagcaac cggctccatc
     3241 gggacgcaag ttttagagat tgtcaagaac aatcccgatt atcggattaa gagtatcgct
     3301 tttgggaaca atgttgtttt aggaaggaaa attattgagg aatttcaacc ggaatttgtg
     3361 gcggtacgga atgatacgat taaagaagat atttttcgcc aatttcccca attaaaaatt
     3421 ggcgttggtg aggccggatt aattgaagcc gcgacctttg ttgcgcccgg agattattta
     3481 ataaatgctt tagtgggtgt tttaggtttg gtaccgacag tggcggcgat tgaaaagggc
     3541 attaatattt tattggctaa taaagaaacc ttagttgtcg gt
//
LOCUS       DAOSGN010000036         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_24318_length_3562_cov_3.070716, whole genome shotgun sequence.
ACCESSION   DAOSGN010000036 DAOSGN010000000
VERSION     DAOSGN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24318_length_3562_cov_3.070716"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PK087_01005"
     CDS             complement(<1..466)
                     /locus_tag="PK087_01005"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOH17878.1"
                     /translation="MKKTFRFSLLLLILISLFNLHIGAASTDVTMVVTNPGPNASTEM
                     NISWHTTNAFAAIQYTKATDPNFQSPSTKLGVCTALPFSGKENTKQCKATLTNLESGT
                     EYLYRIQTTAFSDVYRFKTAGTGPFSFMHITDIHSYLPIPSRVSTANTVLNKA"
     gene            complement(533..1249)
                     /locus_tag="PK087_01010"
     CDS             complement(533..1249)
                     /locus_tag="PK087_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOH17879.1"
                     /translation="MEKLEATRTYWLTTNDFDEGNYLKPAAVLNYFQDIASWHAECLK
                     AGYSHLLAANKIWILLKNKFVLYEHPDFFSRVKLSTWPLTTEKIYYPRAYQMKNDDGK
                     IVGEGMSLWAIINLNTQMVTRDQWFLGTRFKDEQPLSFQKKIASFDQETFIDKYTVRS
                     FDLDHYHHMNNAMYANLIHLVADRLSLLPLTNLEIHYLRQAKINDVISLYYHEDEQNI
                     YITGKDDAHQIIFSSLIEKT"
     gene            1367..3229
                     /gene="mnmG"
                     /locus_tag="PK087_01015"
     CDS             1367..3229
                     /gene="mnmG"
                     /locus_tag="PK087_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOH17880.1"
                     /translation="MYDVIVIGAGHAGVEAALACARVNNKTLMIMSSYQRLANMPCNP
                     SIGGPAKGVIVREIDALGGQMAKVVDQSLLQVKMLNSSKGPAVRALRAQVDKVAYPKM
                     MKDIINNTPNLDLKETFVDAIKVKDGCVSGVVTEHGETILAAAVIMATGTYLSSRILI
                     GNHFWEEGPDHEATSSQLSASLRSLGFSLIRLKTGTPPRIKKDTIDFSKAIPQPGDDK
                     PWRFSEETKEVLPFSQQQLCYLIYTTPLTHQIIHENIHRSAMFSGLIEGVGPRFCPSV
                     EDKIVKFADKERHQIFLEPESLLIDEIYLQGLSTSLPKDVQIPLVHSLPGLEKAEIAK
                     YAYAIEYDAIDPTQLKNNLEAKTIKNLFFAGQINGTSGYEEAACQGLMAGINAHRNLH
                     QLPPLVLRRDEAYIGVLIDDLITKGVKDPYRLLTSRAEFRLLLRHDNAEERLLKHGLD
                     VGLVTKERYERFLEKQRRIDALIDELKKLAITPKPEVNNYLLSQNRAVINEKITGYEF
                     LKRPDNDFLDLRTISKKDLTATEAIEEQVLIKIKYSGYIDKALKEAKKMETLETRKIP
                     ADFDYDQVPNIAKEAKEKLKKVRPATIAQASRISGVNPSDIAILLVWLESKKHA"
     gene            3222..>3562
                     /gene="rsmG"
                     /locus_tag="PK087_01020"
     CDS             3222..>3562
                     /gene="rsmG"
                     /locus_tag="PK087_01020"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOH17881.1"
                     /translation="MLESFNLSKLQQAQFQEYYQFLVSENQKYNLTAITNEQEVYIKH
                     FEDSLAVSKAVDLRKVKTLLDVGSGAGFPGIPLAIVYSHLSVTIVEPTSKKVKFLEQL
                     VRRLSLKNVQL"
BASE COUNT         1110 a          658 c          782 g         1012 t
ORIGIN      
        1 cggctttatt taaaacggta ttagcggtag aaactcgtga gggaatcggt aaataagagt
       61 gaatatcggt aatgtgcatg aaactgaagg gacccgtacc ggccgtttta aagcggtaaa
      121 catcggaaaa agccgttgtt tgaatccgat agagatattc ggttccgctt tccaaattcg
      181 ttaaagtggc tttacattgt ttcgtgtttt cttttccact aaaaggaagg gctgtacaaa
      241 cgccgagttt tgtggaaggg gattgaaaat tagggtccgt ggctttggtg tactgaatgg
      301 cagcaaaggc attagtggtg tgccacgaga tattcatttc cgtactcgca ttgggaccgg
      361 ggttggtgac caccatggta acatcggtgg atgcagcccc aatatgaaga ttgaataagc
      421 ttattaaaat aagtaaaagt aaagaaaacc ggaatgtttt tttcatataa tcctccatac
      481 gccccccatt atatcataag ttgatggtta aaaaaaggct taagcgaaaa acttaagtct
      541 tttcgatgag ggaagaaaaa ataatttggt gggcatcatc tttgccggta atatatatat
      601 tttgttcgtc ttcgtggtaa taaagactga taacatcgtt gattttagct tgccttaaat
      661 agtgaatttc caaattagta agggggagca aggatagacg atcggccact aaatgaatta
      721 aattggcata catcgcatta ttcatatggt ggtaatggtc aagatcaaaa ctgcgaacgg
      781 tgtatttatc gatgaaggtt tcttggtcaa aagaagcaat tttcttttga aaggaaagcg
      841 gttgttcgtc tttaaacctc gttcccaaaa accattggtc acgagttacc atctgggtgt
      901 ttaagttgat gatggcccat agtgacattc cttcgccaac tattttaccg tcatcatttt
      961 tcatttggta ggcccgcgga taataaattt tttctgtcgt taagggccaa gtagaaagtt
     1021 tgacacgaga aaaaaagtca gggtgttcat aaagaacaaa tttatttttc agtaaaatcc
     1081 atattttgtt ggcggccagt aaatgtgaat atccggcttt taaacattca gcatgccaag
     1141 aagcaatatc ctgaaagtaa ttaaggacgg ctgccggttt gagataattt ccctcatcaa
     1201 aatcgttggt tgtcagccaa tatgttcttg ttgcttctaa cttttccatc gtttctctcc
     1261 ttgcgaacaa atctattata acaccaaggt ccttttttcc caagttaaac agtaaaaata
     1321 tcgtgcaaat aatagaaagt atgataaaat gagaatggtg aaaaatatgt atgatgtaat
     1381 tgttattggc gccggccatg ccggagttga agcggcttta gcttgcgcca gagtaaataa
     1441 taaaacctta atgattatga gttcctatca gcgccttgcg aatatgcctt gcaatccttc
     1501 aattggtgga cccgccaaag gggtgattgt tcgtgaaatt gatgctttgg ggggccagat
     1561 ggccaaagtt gtcgatcaaa gcttattaca ggtgaagatg ttaaacagtt cgaaaggacc
     1621 ggcggtgcga gccttaagag ctcaagtgga caaagttgct taccctaaga tgatgaaaga
     1681 catcatcaat aatactccca acctcgattt aaaagaaacc tttgtggatg caatcaaagt
     1741 aaaagatggt tgcgtaagcg gtgttgtgac ggagcacggg gaaacgattt tggcagcggc
     1801 ggtcattatg gcaaccggga cttatttatc atcgcgcatt ttaatcggta atcacttttg
     1861 ggaagaaggg cccgaccatg aagcgacatc tagtcaattg tcggcatcgc ttcgttcgct
     1921 tggcttttcg ttaattcgtt taaaaacggg aactccgccc cgcattaaga aagatactat
     1981 tgatttttca aaggcgattc cccaacccgg cgatgataaa ccttggcgct tcagtgaaga
     2041 aacaaaggaa gtattaccct tttcgcaaca acagctatgt tatttaatct ataccacgcc
     2101 cttaactcat caaatcatcc atgaaaacat tcatcgttca gcaatgtttt cgggtttaat
     2161 tgaaggggtt ggaccgcgtt tttgtccatc cgtggaagat aaaatcgtca agtttgcgga
     2221 taaagaacga caccagattt tcttggaacc ggaaagttta ttgattgatg aaatttattt
     2281 acagggtctt tccacaagcc ttcccaagga tgtccaaatc cccttagttc acagccttcc
     2341 cggcttggag aaggccgaga ttgcgaaata cgcttatgcg attgaatatg atgcgattga
     2401 tccgacccag ttaaaaaaca atttggaagc caaaacaatc aagaatctct tttttgccgg
     2461 tcaaatcaac ggcaccagcg gatatgaaga agccgcttgc caaggcttga tggcgggcat
     2521 taatgctcac cgcaatttac atcaattacc gcccttggtt ttaaggcgcg atgaagccta
     2581 tatcggcgtt ttaatcgatg atttaattac caaaggtgtt aaagatccct atcgtttatt
     2641 aacatcacga gcggaatttc gcttgcttct gcgtcatgat aatgcggagg aacgcttatt
     2701 aaaacatggt ttggatgtcg gcttagtaac aaaagaacgc tatgaacggt ttttggaaaa
     2761 acaacgaagg attgatgcct tgattgacga gttaaagaaa cttgccatta ccccgaaacc
     2821 tgaagttaat aattatcttc taagccaaaa ccgggccgtc attaatgaga aaatcaccgg
     2881 ctatgagttt ttaaaacggc cggataatga ttttttagat ttacgaacca ttagcaaaaa
     2941 ggatttaacc gccaccgaag ctattgaaga acaagtatta attaaaatta aatattccgg
     3001 ttatatcgat aaagccctta aagaagccaa aaaaatggaa actttggaaa caagaaaaat
     3061 ccccgctgat tttgattatg atcaggtacc aaatattgcg aaggaagcga aagaaaagct
     3121 gaagaaagtc cggccggcga caattgcgca agcttcacgc atcagcggcg tgaatccaag
     3181 cgacatcgcc attttattgg tgtggttgga gagtaaaaaa catgcttgag agttttaatt
     3241 tatcaaagtt acaacaagcg cagtttcaag aatattatca gtttttagtt tcggaaaacc
     3301 aaaaatataa tttaacagca ataacgaatg aacaagaagt ctatatcaaa cactttgaag
     3361 acagcttggc tgttagtaaa gctgtggacc ttaggaaagt aaaaaccctc cttgatgtcg
     3421 ggagcggggc cggctttccg gggattccct tagccattgt ttattcccat ttatcagtga
     3481 cgattgtgga accgacaagc aaaaaagtta agtttttgga acagcttgtg agacgattat
     3541 ctttaaaaaa tgtgcaatta at
//
LOCUS       DAOSGN010000037         3543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_24475_length_3543_cov_3.243693, whole genome shotgun sequence.
ACCESSION   DAOSGN010000037 DAOSGN010000000
VERSION     DAOSGN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24475_length_3543_cov_3.243693"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK087_01025"
     CDS             complement(<1..282)
                     /locus_tag="PK087_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOH17882.1"
                     /translation="MMNNEKQIIFYNANSAYFNVHGLPFASKEHPFWRLPDDVFISQT
                     IEFLKQHPSGAYLSFATASPSIRIKASVSSKAYMPHMTATGTFGCDLYIR"
     gene            625..1491
                     /locus_tag="PK087_01030"
     CDS             625..1491
                     /locus_tag="PK087_01030"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOH17883.1"
                     /translation="MDYIAFDFETANKNLTSACSIGLVGVSNNKIAFAIQYLINPEEE
                     FSDYNINVHHITPKLVADAGNFPCVWEKIRPYFTNTVVFAHNVAFDIKVLKALIDKYE
                     LEPPQVKFGCTWKMAAKLWKDELKNHRLPTLAAHLGINHSPHDALSDAKVCVEIITRG
                     QKITSTSDVNSLYEALGIRFGRYDSYRFFPSNYIYVRKTAKTIVKNKALNDKIICVLG
                     KPKTMTKTQLTDKLLSQGVFIEKSINRSLDYVLVLGNCPKAQLMTAIKLTQQGASMKI
                     INEQELLAMMLG"
     gene            1494..2348
                     /locus_tag="PK087_01035"
     CDS             1494..2348
                     /locus_tag="PK087_01035"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH17884.1"
                     /translation="MRELSFIALEDSDVQTFLASFHVAKKTINKMIRNHELAINGQEI
                     NSNMAFKKDDLITVAYYETTNIKPYFQKIKIAYEDDDIIVINKAPSLLIHSDGNSDQT
                     LDNMVYAYFLQKNETINPRHLHRLDVDTSGLVVYAKHLLAHAFLNYQMEQGFIQKRYY
                     AVVEGIMANKQGIINLPIGRNRHQSKQFIVSKQGKPALTEYRVLKEVNHQSLLAISLH
                     TGRTHQIRVHFAHLHHPLVGDQLYGQSSSRLMLHAYFLEFIHPRTRKPLQVVTTIPED
                     FIIKNEED"
     gene            2350..2844
                     /gene="thpR"
                     /locus_tag="PK087_01040"
     CDS             2350..2844
                     /gene="thpR"
                     /locus_tag="PK087_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOH17885.1"
                     /translation="MRAFIGIKPDGCLADLIAIQNDLKQQTKANFTKPDNLHITLFFL
                     GEIDEGQTQKIKRLFQNFSFPRFSLEINAITNFREMIIATLSPSLALKQLHKKLGDCL
                     MRDGFLLENRPYFAHLTLARKTDYVVNKAVLLTTTVENIVLFASERQETGLVYRRIVD
                     VKLN"
     gene            2868..>3543
                     /locus_tag="PK087_01045"
     CDS             2868..>3543
                     /locus_tag="PK087_01045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOH17886.1"
                     /translation="MKTNFKEAEQFPSFYSDPFELRLARNAQEINEVFKLRYEDLLLY
                     YNAENTNETGLFTDQYDDFCEHLIVIDHTEEVIAGTYRFVRKEHLINTDLFITEHEYD
                     LTLLKPYRIMELGRAVVRKEYRSGAVIMLLWRGIFEYAKRNNIQYMFGTASFNGLNVR
                     DYEHGLSYLYYHHLSKFAIPALGENPLKLNILPLEEVDEGLAKRQLPPLVKGYLRIGA
                     KFGPDIF"
BASE COUNT         1152 a          643 c          670 g         1078 t
ORIGIN      
        1 gcgaatgtat aaatcgcagc cgaaggttcc ggtggctgtc atgtggggca tataggcttt
       61 tgaagaaacg gatgccttaa tgcggataga aggagaagca gtggcgaaag acaaataagc
      121 cccactggga tgttgtttta agaattcgat agtttgggaa ataaagacat catccggtaa
      181 tcgccaaaac ggatgttctt tactggcaaa cggtaagcca tggacattaa aataggcgga
      241 attggcatta taaaaaatta tttgtttttc attattcatc attttatcac ctgaagtatt
      301 atattacaaa tagcctcgaa aagaaagacc aaagtaatta gaatttacat aaaatttgtt
      361 tctgaacaag cgatcatgta cacaaacgaa aataacgtca tttaaaggat aaaatacttg
      421 catttattaa ggaaatatag tatactattg ttgcgccgag aggcgtaact tatcccctac
      481 atatagcagt gaataagcaa ttattcacaa catccccttc ctaaaactga gccttacccc
      541 ggctcagttt ttcctttttt aatcgacttc ttacatagac aaaaactctt ttccttgtta
      601 taatatatca aagtaggtca ccttatggat tatattgcct ttgattttga aactgccaac
      661 aaaaatttaa ccagtgcttg cagtattggt ttggttggtg taagcaacaa taaaattgcc
      721 tttgctattc aatatttgat taatccggaa gaagaattca gtgattataa tattaacgtt
      781 catcatatta cccctaaatt ggtcgccgat gccggtaact tcccttgtgt ttgggaaaag
      841 attcgtcctt actttaccaa tacggtggtg tttgcccata atgtggcctt tgatatcaag
      901 gttttaaaag ccttaattga taaatatgaa ttagaaccac cgcaagtaaa atttggctgt
      961 acttggaaaa tggccgccaa actgtggaaa gatgaactga aaaaccaccg tttaccaacc
     1021 ttggctgctc atttgggaat taatcattca cctcatgatg ctttaagtga tgcgaaggtc
     1081 tgtgtggaga ttattactcg tgggcaaaaa ataacaagca ccagcgatgt caacagttta
     1141 tatgaagcat tgggaattcg tttcggtcgc tatgattcat atcgcttttt tccaagcaat
     1201 tatatttatg tccgcaaaac cgcaaaaaca atcgttaaaa acaaggctct taatgataaa
     1261 atcatttgtg ttttgggaaa accgaaaacg atgacgaaaa cccaattaac tgataaacta
     1321 ctttctcaag gtgtttttat tgaaaagagc attaaccgca gtttagatta tgttttggtt
     1381 ttgggtaatt gtccgaaagc ccaattaatg accgcaatca aattaacaca acaaggagca
     1441 agcatgaaaa tcatcaacga acaagaattg ttagccatga tgctaggata acgatgcggg
     1501 aattaagttt tattgcttta gaagatagtg atgtccaaac cttcttggca tcatttcatg
     1561 ttgcgaagaa aacaattaat aaaatgattc gtaaccacga actagccatt aacggtcagg
     1621 aaattaacag caacatggct tttaaaaaag acgacctcat tacggttgct tattatgaaa
     1681 caactaatat caaaccctat ttccaaaaaa taaaaattgc ttatgaagat gatgacatca
     1741 tcgttatcaa taaagcacca tcacttttaa ttcactcgga tggtaatagt gaccaaactt
     1801 tagacaatat ggtatatgcg tatttcttac aaaaaaacga aactattaat cctcgtcatt
     1861 tacatcgttt agatgttgat accagtgggc ttgttgttta tgccaagcat ctcttggctc
     1921 atgcgttcct aaattaccaa atggaacaag gcttcataca aaaacgctat tatgcggttg
     1981 tagaaggtat aatggcgaac aaacaaggga ttatcaatct accgattggc cgtaaccgcc
     2041 atcaatcaaa gcaattcatt gtcagcaaac aaggaaaacc ggccttaact gaatatcgag
     2101 ttttaaaaga agtcaatcat caatcgttat tggcgattag tttgcacacg gggagaacac
     2161 atcaaattag agttcatttc gctcatttac accatccgct tgttggtgat caattatatg
     2221 ggcaatcgtc ttctcgttta atgctccatg cttatttttt agaattcatc cacccacgaa
     2281 caagaaaacc acttcaggtt gtaacgacga tacctgagga ttttatcatc aaaaacgaag
     2341 aggattaaaa tgcgcgcttt tattggtatt aaacctgatg gctgtttagc agatttaatc
     2401 gccatccaaa acgacttaaa acagcaaaca aaagctaatt tcacaaaacc ggataattta
     2461 catatcacct tgtttttctt gggtgaaatt gacgaagggc aaacgcagaa aatcaaaagg
     2521 ctttttcaaa actttagttt tccgcgcttt tcattagaaa ttaatgctat tactaatttt
     2581 cgggagatga ttattgcgac cctaagccct agtttagcac tgaaacaact gcataaaaaa
     2641 cttggtgatt gcttgatgag ggatggtttt ctccttgaaa accggcctta ttttgctcat
     2701 ctgaccttag caagaaaaac tgattatgtc gttaacaaag cggtgttgtt gacaacgacg
     2761 gttgaaaaca ttgttttatt tgcctcggaa cgacaggaaa ccgggcttgt ctaccgtagg
     2821 attgttgatg ttaaacttaa ctaagcaaga agggagttaa ttaatctatg aaaactaact
     2881 ttaaagaagc tgaacaattt ccttcttttt attctgaccc ctttgaactg agattagcaa
     2941 gaaatgcgca ggaaattaac gaagttttta aattgcgcta tgaggatttg ttgctttatt
     3001 acaatgctga aaacaccaat gaaaccggct tgtttaccga ccaatatgat gatttttgtg
     3061 agcacttaat cgtcattgat cacacggaag aagttatcgc cggcacttat cgttttgttc
     3121 ggaaagaaca cttaattaac accgatttat ttattaccga gcacgagtat gatttaacat
     3181 tgttgaaacc ctaccgcatt atggaattag gtcgtgccgt tgttcgcaaa gaatatcgaa
     3241 gcggggcggt tatcatgttg ttatggcgcg gtatttttga gtatgccaag cggaataata
     3301 ttcagtatat gtttgggacc gcttcgttta acggtcttaa tgtgagagat tatgagcatg
     3361 gactttcgta tttgtattat catcatttat cgaagtttgc tattcctgcc cttggagaaa
     3421 accctttaaa gttaaatatt ttaccgcttg aagaagtcga tgaaggctta gccaaacgtc
     3481 aattgccacc gcttgttaag ggctatctac gaatcggggc gaaatttgga cctgacatct
     3541 ttt
//
LOCUS       DAOSGN010000038         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_25052_length_3485_cov_2.854519, whole genome shotgun sequence.
ACCESSION   DAOSGN010000038 DAOSGN010000000
VERSION     DAOSGN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25052_length_3485_cov_2.854519"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PK087_01050"
     CDS             complement(<1..1059)
                     /locus_tag="PK087_01050"
                     /inference="COORDINATES: protein motif:HMM:NF011547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOH17887.1"
                     /translation="MAENTKTLTLIDGNSLMFRSYYATAYTNNLMKTKDGLYTNALFG
                     FCNMLNKMLTEELEYAFVAFDAGSKTFRHQQFTTYKSTRKPLPEELRVQIPLIKQYLD
                     ILKVKRAESLDFEADDLLASVAVHSKKAFQKIHIITGDRDLLQMVDDKISVFLTKKGI
                     GELDEYNQANFYEKMQLHPFQIVEYKAIVGDASDNLPGIKGLGNVSAIKLLNQYQTLD
                     NIYEHLDELTPKMKQAFIENKEIAYQCRFLASLKRDILFDWPLSELKIGHFDLHELVA
                     FFQKVEFNHFLKKLNKQHEGPSKDIIFVKDALTPMQVDSNEALYVSAEVFGSNYYTGT
                     FLGLGLVNAFQQWFVTAEA"
     gene            complement(1151..3358)
                     /locus_tag="PK087_01055"
     CDS             complement(1151..3358)
                     /locus_tag="PK087_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17888.1"
                     /translation="MKHHRQLIIFIFFLTILSACSFSPSAKTEKVFQGLFWGADLTRV
                     TSDLFFPKQVDGVSLSWSSNNEEVIDNQGHVFRAEGDVTVVIDVVLEYQGYTDHRQLL
                     VTVLKRSFYPISKAKSIGDQKTVTVNGTVIGTVGHDAYLHDGRDGILVKNIGDVELGA
                     FLLVTGIKQVINGQLQLLFVEKTVDENIDFVIKSQTIADFTLLNQVNDMVTIESVTMI
                     VKESSYSSDVRVELINQNQQSMELLIRATHANYQTLIEQIAQLPSNNRVHLHQVIVSS
                     LNPRQVEFVQESSLESLNINLQAAFYPEPGSVSLLEDLLIETEITAGLPSLNDVHALI
                     IPVEFADYSFTQVDLERLELAFFGTAAETGWESVQSYYQQSSYGKLQFNGTVLPPFQT
                     HRLASYYSRLFKKGIDADYEIVKAALEYYDSQIDYSEYDRNNDGYIDALYFIYAAPVN
                     FKGSWFSLNNVDLWWAYVYQYLSDDYEYYDGVEANYYLWAGLDFINEPLIDEGNNKQM
                     IPINASTYIHETGHMFGLDDYYDYNEFKGPDGGLGGADMMDYTVGDHNPFSKIILGWT
                     TPLVVTEESVTVTLRPFSESGDVIMINPSWENSYFDEYLLIDFYVPSFLNEAHAGYRG
                     LFSESGIRIFHVDATADPKQGSPQNENGYYSVFSFNNSDTDHKLIKLIEADGNYSIEK
                     TGVADNADLYRPGDIFGKTSYPGYRWYDRTLINFTVEIISISDDEAIIMISFK"
BASE COUNT         1132 a          715 c          587 g         1051 t
ORIGIN      
        1 cgcttccgcc gtcacaaacc actgctgaaa agcattaacc aaacccaaac ctaaaaaggt
       61 gccggtataa taattgcttc cgaaaacttc agcactgaca taaagagctt cgtttgagtc
      121 gacttgcata ggtgtcaagg catccttcac aaaaatgata tccttgcttg gtccttcatg
      181 ctgcttattt aatttcttca aaaagtgatt aaattccacc ttttggaaaa aggcgaccaa
      241 ttcatgcaag tcaaaatgac caatttttaa ctcggacagt ggccaatcaa acaaaatatc
      301 tcgttttaaa ctggctagaa agcgacattg ataagcaatc tctttgttct caataaaggc
      361 ttgtttcatt ttcggtgtta actcatctaa atgttcataa atgttatcaa gcgtttgata
      421 ttggtttaaa agtttgatgg cactgacatt cccaagacct ttaatccccg gtaaattatc
      481 ggaagcatcg ccgacaatcg ctttgtattc tacaatctga aagggatgaa gttgcatttt
      541 ttcatagaaa ttggcttgat tatattcatc gagttcgccg ataccttttt tggttaggaa
      601 aacggaaatc ttatcatcca ccatttgcaa taaatcccga tccccggtaa taatgtgaat
      661 tttttgaaag gccttcttgc tatgaacggc cacactcgct aacaaatcat ccgcttcaaa
      721 atcaaggctt tccgctcttt taactttaag aatatctaaa tattgtttaa taagcggaat
      781 ttgcactcgc aactcttccg ggagcggttt tctggtgctt ttgtaagtag taaactgttg
      841 atggcggaaa gttttactgc cggcatcaaa ggctacaaag gcatattcga gttcttcagt
      901 taacatctta ttgagcatat tacaaaagcc aaacaaggca ttagtatata agccgtcttt
      961 cgttttcatt aaattgttgg tatatgctgt ggcatagtaa gaacggaaca ttaaactgtt
     1021 gccatcaatc agcgttaagg ttttagtgtt ttctgccata atttcctacc tttatcttca
     1081 agttatttta tcatataatt taaaaaattt caccctcaaa taaataaatg attctcttct
     1141 aaagaatcat ttacttaaag cttatcataa taatcgcttc atcatcgcta atgctgatga
     1201 tttccacagt aaagttaatc aatgttcgat cgtaccaacg ataaccggga taactggttt
     1261 taccgaagat atcacccggt cggtataaat cggcattatc ggcaactcca gttttttcaa
     1321 tcgaataatt accatcggct tcaattaact taatcagctt atggtcggta tcgctattat
     1381 tgaaagaaaa gactgaataa taaccattct cattttgggg cgaaccttgc ttggggtcag
     1441 cagttgcatc aacatggaag attcttattc ctgattccga gaacaaaccc cgataaccgg
     1501 catgagcttc attcagaaaa ctaggaacat agaaatcaat taacaaatat tcatcaaaat
     1561 aactattttc ccaactggga ttaatcataa taacatcgcc actttcggaa aacggcctta
     1621 aggtcacagt caccgattct tcggtcacaa cgagtggtgt cgtccaaccg aggataattt
     1681 tcgaaaacgg gttatggtcg ccgaccgtat aatccatcat gtccgctcct cccaaaccac
     1741 catcgggacc cttaaattca ttgtaatcgt aataatcatc taaaccgaac atatgtcccg
     1801 tttcatgaat ataggttgaa gcattaatcg gaatcatttg tttattatta ccttcatcaa
     1861 tcaagggttc attgataaaa tccaaacccg cccataaata gtaattagct tctacaccgt
     1921 cataatactc atagtcatca cttagatatt gataaacata tgcccaccat aaatcaacat
     1981 tgtttagtga gaaccaagaa cctttaaaat taaccggggc cgcataaata aaataaaggg
     2041 catcgatata accgtcgtta ttgcggtcat attcagagta atcgatttga ctatcataat
     2101 attctaaagc agccttgacg atttcgtaat cggcatcaat ccctttttta aaaagtcgag
     2161 aataataaga ggccaaacga tgcgtttgaa aagggggtaa aaccgttccg ttaaattgca
     2221 gttttccata cgaactttgt tggtaataac tttggacact ttcccatccg gtttcggcgg
     2281 ctgtcccaaa aaaagctagc tccaaacgtt ctaaatccac ttgggtaaaa gaataatcag
     2341 caaattctac cggaattatc aatgcatgaa catcatttaa gctcggtaaa ccggccgtga
     2401 tttcggtttc aatcagtaaa tcttcaagca aagaaacgga acctggttca gggtaaaaag
     2461 cggcttgtaa attaatattt aaactttcta aacttgattc ttgtacaaac tcaacttgac
     2521 gaggatttaa actgctgaca atgacttggt gtaaatgaac tcggttatta gaaggcaact
     2581 gagcaatttg ctcaataaga gtttgataat tggcatgcgt tgctctaatc aacaattcca
     2641 ttgattgttg attttgatta ataagttcaa cccgaacatc gctagaataa ctactttctt
     2701 tcacaatcat cgtaacggac tcaatcgtaa ccatgtcatt aacttggtta aggagcgtaa
     2761 aatcagctat cgtttgggat ttaatgacga agtcaatgtt ttcatcgact gttttttcca
     2821 caaacaaaag ttgtaactgc ccgtttataa cttgttttat tccggttaca agcaaaaatg
     2881 ctcctaattc cacatcgccg atattcttta ccaaaatccc atccctacca tcatgaagat
     2941 aagcatcatg accaacggtg ccaatgacgg tcccattaac ggtgacggtt ttttgatcac
     3001 caatgctttt cgctttacta atggggtaaa acgaacgctt taataccgtt actaataatt
     3061 ggcgatggtc cgtataacct tgatattcca aaacaacatc aatgacgacg gtgacatctc
     3121 cttcagccct aaaaacatga ccttgattat cgataacttc ttcattgttg gaagaccaag
     3181 agagggaaac accatccact tgtttaggga aaaacaaatc gcttgtgact ctcgttaaat
     3241 cagctcccca aaataagcct tgaaaaacct tctccgtctt agcggatgga gaaaatgaac
     3301 aagcacttaa gatggttaaa aagaaaataa aaatgatgag ctgacgatga tgtttcatat
     3361 tccacctata tgaattatta taactgatta ttaaaagaga agcaattatt tctttttgag
     3421 aaaacaacgc ggcatcttcc aagcctaaat gcgacaaata aacaattgaa aaagttcata
     3481 gaaat
//
LOCUS       DAOSGN010000039         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_25134_length_3477_cov_2.789597, whole genome shotgun sequence.
ACCESSION   DAOSGN010000039 DAOSGN010000000
VERSION     DAOSGN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25134_length_3477_cov_2.789597"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            545..1729
                     /locus_tag="PK087_01060"
     CDS             545..1729
                     /locus_tag="PK087_01060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOH17889.1"
                     /translation="MMALQNILIASSNNGIVLEEILVANAIGLLVLIVSLLSRVEIKK
                     ERHLSGKIFDGMVWITFFALILETVTFLLDEKPGALVHFLQYATNTYLFLASSLVGIL
                     WVLFVDIRIFRSSTRIKWWLKFLIIPYIALIVLIIFDYFGADIIFSITEQNKYDRGKF
                     VMLTFGFVFCCYFITLALAVLAVKRDGHIRFFPVHYFVIPSLLGTLVQGMFYGLAAGW
                     LCLSIALLFVQLHLANQNAYEDELSGLYNRKYFGWMIEKLAKGKKNRFISAIMMDIDR
                     FKLINDEFGHSVGDDAIRSIGKLLTDITSDDVIAFRVGGDEFTVLHIGGTEADIEQLT
                     FIINERISDFNKTAKKPYSLSVSMGYSTCQTTGTSLDFFFHQLDQKMYEQKALQYAKN
                     QK"
     gene            2062..2238
                     /locus_tag="PK087_01065"
     CDS             2062..2238
                     /locus_tag="PK087_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17890.1"
                     /translation="MAFAIKLGLNKVEEAADNLIRVCQDIVNNGGKAPYLSVLYMVLE
                     TGLIKTTMVFSLFP"
     gene            2356..2592
                     /locus_tag="PK087_01070"
     CDS             2356..2592
                     /locus_tag="PK087_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17891.1"
                     /translation="MAEKTSIALRNLFFYQVFVRQFSETHDFHGLIKQLDRLKSLNID
                     VIQLLPIHPIGQLKRKGTVGVPTPLAITMKSILI"
     gene            2598..>3477
                     /locus_tag="PK087_01075"
     CDS             2598..>3477
                     /locus_tag="PK087_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH17892.1"
                     /translation="MDDFTLLLAESHKRGMKVIIDIVFNHTSHDAKYTKTHPHWYYHK
                     ADGSFANRVGDWWDIIDFNFENNEALEKELINVLIYWTKIGVDGFRCDVAPLLPLRFW
                     KKARQELETVNPNLIYVSESVHLSFIKYLRDLAYDAFSDSEIFQVFDVCYDYDIFDDF
                     QNYFQGKGKLKTWITSLIRQESSYPKNYVKLRYLENHDMERLAKYLKTDSQLRTVNAM
                     LFFLKGIPFIYNGQECGAHNRPDLFEIDEIDWQDYNRAGIVDIIKKVGALRKQWEFVN
                     WKMEINQYNSDTMEIKY"
BASE COUNT         1093 a          516 c          664 g         1204 t
ORIGIN      
        1 tgttctttaa atcttttttc tattttcatt ttacaatatt catatatttt tttcttatat
       61 ctgtcgcctt tctaattaga atctacatgt ataaatacat agaagaacaa gtgtcttgat
      121 aagatttttt atcaagcctt ttttggtttg taaaatttta ttttttgaat tgcatttttt
      181 gattattttt agtataataa cgataattag aataataatt aagtgggatt gcccttgctt
      241 aatgtacaag aagcagggtt taatccgtca attcttccat aagagaatgt ttactttgat
      301 aaaatcaaag actaaagtta taagttaaat aggctttttt ggttaaaata agtaaaaact
      361 ctaataatta tcaattgaag tcatttagag ttttttgttt tgggttgctc ttttaaataa
      421 ttgattatta ccccaatgac agcatccggt ggtgttgtga aaacgacggt tttgtattac
      481 taggcacgat agttatttat ctctatgagg taaataattg aaaattgaga ggatagtgaa
      541 aaagatgatg gcattacaaa acattttaat agctagttca aataacggga ttgtattaga
      601 agaaattctc gtagccaatg caattggttt gcttgtgctg attgtttcct tgctatcccg
      661 agttgaaata aaaaaagaaa gacatttgag cgggaaaata tttgacggaa tggtgtggat
      721 tacctttttt gcgttgattc ttgaaacagt aactttcttg ttggatgaaa aaccgggcgc
      781 tttggttcac ttcttgcaat atgcaaccaa cacttatctt ttcctagctt caagccttgt
      841 gggtattctt tgggttttgt tcgttgatat tcgcattttt cgcagtagta ctcgtatcaa
      901 atggtggtta aagtttttaa tcattccgta tattgcttta attgtgttaa ttatcttcga
      961 ctatttcggg gcggatatca ttttctcaat cacggaacaa aataaatatg accgaggtaa
     1021 gtttgtcatg ctaacattcg gttttgtgtt ttgttgttat tttataacct tggctcttgc
     1081 cgttctcgcc gttaagcggg acggtcatat tcgctttttc ccggttcact actttgtaat
     1141 accttcgctt ttaggaactt tggttcaagg gatgttttat ggattggcgg ccgggtggct
     1201 atgtttaagc attgcgttgt tgtttgtcca gttgcatctt gctaaccaaa atgcatatga
     1261 ggacgaactt tcgggcttat ataaccgtaa atactttggt tggatgattg aaaagttagc
     1321 taaaggtaaa aagaatcgtt ttatcagcgc tattatgatg gacattgacc gctttaagct
     1381 tataaacgat gaattcggcc attcagttgg tgatgatgct attcgcagca tcggtaagct
     1441 tttgacggat attacctcgg acgatgtaat tgctttccgg gttggcggtg atgaattcac
     1501 tgttcttcac atcggtggaa cggaagccga cattgagcaa ttaactttta taattaatga
     1561 acgaatatcc gatttcaaca aaacagcaaa aaaaccctat tcattatccg tttcgatggg
     1621 gtattcaacc tgccaaacga cgggcacgag ccttgacttc tttttccatc aattggatca
     1681 gaaaatgtat gaacaaaaag ctttgcaata tgcaaaaaat caaaaataag caagaagaaa
     1741 agatgttgcg agttggcaag caaatcgagc caaatagctt ggcaagctta acaaaaatat
     1801 caaggaccca aaataattta ttattaaaaa tcgaaggagc tggttgctcc ttttattcat
     1861 atataatctt aagaagattt taggtattaa aaacgcagtt tgtaaaatgt tggtcagttt
     1921 gattgatgaa tattggtcag tttgttataa taattataga atttacatgg agatattact
     1981 cttataaaaa acagttgaca aatttaaata tataaaggca aagaaattga tgcagtaatt
     2041 gaacttgatg atggaaattg gttggctttt gcgattaaac ttggtcttaa taaagtagaa
     2101 gaagctgcag ataatttaat tagggtttgc caagacatag ttaataatgg tgggaaggca
     2161 ccatacttaa gtgtgttata tatggtgctg gaaacagggc ttataaaaac aacaatggtg
     2221 ttttcattgt tcccatagca tctttaaaat attaatagta aaacaattct gtttaaataa
     2281 aatagcaagc agaaaaactg aacatcgttt atttttaaat ttaatctaat ataatgacat
     2341 taagaaggag aatttatggc tgaaaaaact tccattgcac ttagaaattt atttttttat
     2401 caggtttttg ttcgtcaatt ttcagaaaca catgattttc atggtcttat caagcaactt
     2461 gatcgcctaa aaagcctaaa tattgatgtt atccagttgt tgcccattca tccaattggt
     2521 caacttaagc gtaaagggac ggtgggagtc cctactccat tagcgattac tatgaaatca
     2581 atcctgattt aggaactttg gatgatttta cattgctttt agcagaaagc cataaacgag
     2641 gcatgaaagt cattattgac attgttttta atcatacttc ccatgatgca aaatacacga
     2701 agactcatcc tcattggtac taccataaag ctgatggttc attcgccaat cgagtcggtg
     2761 attggtggga tattatcgat tttaattttg aaaacaatga agctttagaa aaggaattga
     2821 ttaatgtttt aatttattgg acaaaaattg gcgttgacgg ttttcgctgc gatgttgccc
     2881 ccttgcttcc gcttcgtttt tggaagaagg cccgtcagga attagaaaca gtcaacccga
     2941 atttaatcta tgtttccgaa tccgttcact tatcgtttat taaatatctt cgtgatttag
     3001 cttacgatgc cttttccgat agtgaaatct tccaagtttt tgatgtttgc tatgattatg
     3061 acatctttga tgattttcaa aattatttcc aagggaaggg caaattgaaa acatggatta
     3121 ctagtctcat ccgccaagaa tcaagctatc ccaagaatta tgtgaaatta agatatttag
     3181 aaaatcatga tatggagcga ttggcaaaat atttaaagac tgattcacaa ttacgaactg
     3241 tcaatgcgat gttgtttttc ttaaaaggga ttccgttcat ttataatggt caagaatgcg
     3301 gcgcccacaa ccgtccggat ttgtttgaaa ttgatgaaat tgattggcaa gattataacc
     3361 gagccgggat tgttgatatt attaaaaaag tcggtgcttt aagaaaacag tgggaatttg
     3421 tcaactggaa gatggagatt aatcaatata atagcgacac tatggaaatc aagtata
//
LOCUS       DAOSGN010000040         3468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_25254_length_3468_cov_3.034281, whole genome shotgun sequence.
ACCESSION   DAOSGN010000040 DAOSGN010000000
VERSION     DAOSGN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25254_length_3468_cov_3.034281"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PK087_01080"
     CDS             <1..242
                     /locus_tag="PK087_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17893.1"
                     /translation="KALEFLQSPVGFAVFIVLPTFLVLVYEGILLARNILSINKAKME
                     AKHQEDMKLVQEQLEKEKEALRAKIMEEMKQEEKK"
     gene            274..1818
                     /locus_tag="PK087_01085"
     CDS             274..1818
                     /locus_tag="PK087_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17894.1"
                     /translation="MVEFSRYYINFIKDFFANIGTFFKTLFEAFADFFFKDIVRYFEN
                     LVNYSNKFNFLDWIATLVVLIINSALVFFLILRIYQFLRRYIRFVKREIEKDELMEEI
                     TALNQKAAELIDEKNKILAMKVSTLGIGTNKMSEDDLAFDDFTKKPAEQTTSRFVKLI
                     SVDEAYAQTVSTITMKSKDMIGLKELVQRFVHFSASQLKLYYDKKIIAAYFAGMATSK
                     IIILEGISGTGKTSLPYAMGKFFSNDAAIVSVQPSWRDRAEMIGYLNEFTKKFNESDF
                     LKSLYETTYREDLNFIILDEMNLARIEYYFAEFLSILEMPNINEWKIDVVPNNVPGDP
                     KNLVNGKILVPQNVWFVGTANKDDSTFTITDKVYDRAIAIEMNEKAGYIDAPQTESVV
                     MSYEYLDSLFKVAEAEHAMSPKMLDNLAKIDEFITAKFKITFGNRILKQIKQFVPVYV
                     ACGGTEIDGLDFMVARKIIRKFESLNLPFLQNEMDQLIVLIEKIFGKNSFGETINFIN
                     ELKKNF"
     gene            1835..>3468
                     /locus_tag="PK087_01090"
     CDS             1835..>3468
                     /locus_tag="PK087_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17895.1"
                     /translation="MRKTDELDKFYAEIIASIKDQESNAQFPEFFYNILLAGDNKIYQ
                     KVITETKRFDGEWIQTLESYFPSLDRIIKNPKSALKYEEEITAIERAKKTTGASIRHL
                     AANTHFIKEIRDDNVVIPKKILTVSAEQDYQIYENRFIMTLINRLSSFVQNRYEVIKS
                     NYESFQRNHVNFKSNFDINDSVVDMDIDLVIKRNLDDDAINQHNLDLLQRAEYLHNLV
                     GGFKNSPFMKLMEKARRVVPPIMKTNVILKNPDFRNAYTLWLFLDRYTILGFDNEVAE
                     KNLPVQRKYLKNIYRLALLTYTTVVFNQRQRMEDYRRIELVTYRKKGTRVAKTHVDDV
                     VKNPDAIRIEDNAINEYYLNQNKKVFKKRLEDIISENFSYDVALRRALNQTIDITNAL
                     YQAVFELEEEEDIFKMLIKEVNLDDKLDDAKKKARIARIIRETKEVDYNRSIRLEKKL
                     MREIDSLNRKLIARDKQLAKDKIDERRLTLMQQERERARNNIKVMDEELKAVAVNKDE
                     LSKQRQAIQQQFKEKAKELKEKKKELLRKEKEKAAN"
BASE COUNT         1203 a          540 c          665 g         1060 t
ORIGIN      
        1 gaaaagcatt agaattctta caatcaccgg ttggttttgc cgttttcatt gttttgccaa
       61 cttttttagt gttggtttat gaaggaattt tgttagctag gaatattctt tccattaata
      121 aagctaaaat ggaagctaaa caccaagaag atatgaaact agttcaagaa caattagaaa
      181 aagaaaaaga agctctaaga gctaaaataa tggaagaaat gaaacaagag gaaaagaaat
      241 agttatttat tcctttaatg aaaggttgaa gttatggtag agttttctcg atactacatt
      301 aatttcataa aagatttttt tgcgaatatc ggtacgtttt tcaaaactct ttttgaggcg
      361 tttgccgatt tcttttttaa ggacatcgtt cgttattttg agaacttagt caattacagc
      421 aataaattta actttttgga ctggattgcg actttagttg tccttattat taattccgct
      481 ttagttttct ttttaatatt acgaatttat cagtttttaa gaagatatat aagattcgtt
      541 aagagagaaa tcgaaaaaga tgaattgatg gaagaaataa ccgccttaaa tcagaaagcc
      601 gctgaattaa ttgacgaaaa gaacaagatc ttggcgatga aagtttcgac tttagggata
      661 ggcactaata agatgagcga agatgattta gcatttgatg atttcacaaa aaaaccagcg
      721 gaacaaacta cttccaggtt tgtgaaattg attagtgttg atgaagctta tgcgcaaacc
      781 gtttccacaa tcaccatgaa gagcaaagac atgattggtt taaaggaact cgtgcaaagg
      841 tttgttcatt tttccgcttc gcaattaaaa ctttattatg ataaaaagat tattgctgct
      901 tattttgccg gcatggccac cagtaagatt attattttgg aaggtatttc cggtaccggt
      961 aaaacctctt taccttatgc catgggtaag ttcttcagca atgatgctgc cattgtttcc
     1021 gttcaaccgt cttggcggga ccgagcggaa atgattggtt atttgaatga atttactaaa
     1081 aaattcaatg aatcggattt tttgaaatct ttatatgaaa caacataccg cgaagattta
     1141 aatttcatca tccttgatga aatgaacttg gcgcggattg agtattattt tgcggaattc
     1201 ttatcgattt tggaaatgcc gaatattaat gaatggaaga ttgacgttgt tccgaataat
     1261 gttcccggcg acccgaagaa tttagttaat ggcaagattt tagttcccca aaatgtttgg
     1321 tttgtgggca ccgctaacaa agacgattcg acctttacca ttacggataa agtttatgac
     1381 cgggcgattg ccattgaaat gaatgaaaaa gccggttata tcgatgcccc gcaaacggaa
     1441 tctgtcgtta tgagttatga gtatttagac agtttgttta aggttgcgga agccgaacat
     1501 gcgatgtccc ccaaaatgtt ggacaactta gcgaagattg atgaattcat taccgctaaa
     1561 tttaagataa cttttggtaa ccggatttta aaacaaatta aacaatttgt tccggtttat
     1621 gtcgcctgcg gcggaacgga aatcgatggt ctggatttca tggtggctcg caaaattatt
     1681 cgtaagtttg agtccttaaa cttaccgttc ttacaaaatg aaatggatca attaattgtt
     1741 ctaattgaaa aaatctttgg gaagaattcc tttggcgaaa ccattaactt cattaatgaa
     1801 ttaaagaaaa atttctagtt agtaggttgt aaacatgaga aaaaccgatg aattagacaa
     1861 attttatgcg gaaataattg cttcgataaa agaccaggaa agcaatgccc aattcccgga
     1921 attcttttat aatattttat tagccggtga taacaaaatc tatcaaaaag tcattaccga
     1981 aaccaagcgt tttgatggtg aatggataca aactctggaa tcgtatttcc ctagtttaga
     2041 tcggattatc aaaaatccga aatcggcttt aaaatatgaa gaagaaatca cggccattga
     2101 aagagcgaag aaaactacgg gcgcttccat tcgtcactta gcggctaata cccatttcat
     2161 caaagaaatc cgtgacgata atgttgtcat tcccaaaaag attttaacgg ttagtgcgga
     2221 acaggattat cagatttatg aaaatcgttt tatcatgacc ttgattaatc gcttatcttc
     2281 ctttgttcaa aaccgttatg aagtcattaa atctaactat gaatcgtttc aacgaaacca
     2341 tgtcaatttt aaatcgaatt ttgatatcaa cgactccgtc gtcgatatgg atatcgattt
     2401 agtcatcaag cgcaatcttg atgacgatgc cattaatcaa cataaccttg atttattgca
     2461 aagggctgaa tatttacata atttagtcgg cggttttaaa aattctcctt tcatgaaact
     2521 aatggaaaaa gcgcgaagag tcgttccgcc catcatgaaa accaatgtga ttttgaaaaa
     2581 tcccgatttt cggaatgctt acactttgtg gcttttccta gaccgttata cgattttagg
     2641 ctttgataat gaggttgcgg aaaagaattt accggttcaa aggaaatatt taaagaatat
     2701 ttatcgttta gctttgttga cttatacaac tgtcgtcttt aatcaacgcc aacgcatgga
     2761 agattaccgt cgaattgaat tagttaccta ccgcaaaaaa ggcactaggg tagcgaaaac
     2821 ccatgtcgat gatgttgtga agaacccgga tgcaattaga attgaagata atgccattaa
     2881 tgaatattat cttaatcaaa ataaaaaagt ctttaagaaa cgtttagaag atattatttc
     2941 ggaaaatttc agttatgatg tcgctttaag gcgtgcttta aatcaaacga ttgacatcac
     3001 taatgccttg tatcaagccg tttttgaact ggaagaagaa gaagacattt ttaaaatgtt
     3061 gattaaagaa gtcaatcttg atgataaatt agacgatgct aaaaagaaag cccgcattgc
     3121 ccgcattatt cgagaaacca aagaagtcga ttataaccgt tccatccgtt tagaaaagaa
     3181 actgatgagg gaaatcgaca gtttaaatag gaaattgatt gctcgcgata aacaattagc
     3241 gaaagataag attgatgaac gaagattaac tttaatgcag caagaacgtg aacgggctcg
     3301 taataatatc aaagtaatgg atgaagagtt aaaagctgtt gctgttaata aagacgaatt
     3361 atcaaaacaa agacaagcca tccaacaaca atttaaagaa aaagctaaag aactaaaaga
     3421 aaagaaaaaa gaattgttgc ggaaagaaaa agagaaagct gctaacta
//
LOCUS       DAOSGN010000041         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_25458_length_3448_cov_2.195402, whole genome shotgun sequence.
ACCESSION   DAOSGN010000041 DAOSGN010000000
VERSION     DAOSGN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25458_length_3448_cov_2.195402"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PK087_01095"
     CDS             complement(<1..324)
                     /locus_tag="PK087_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH17896.1"
                     /translation="MKKLLSIISVFVLMLMIVGCSEQTTATTGEVIKFDETQVKAQVI
                     RVWMDDSEGVFMDEIIPAFEKLNPGIKVEFQHMGTVDIREKLKTYGKSGKGADVFQFP
                     HDHMAQ"
     gene            complement(343..2763)
                     /locus_tag="PK087_01100"
     CDS             complement(343..2763)
                     /locus_tag="PK087_01100"
                     /inference="COORDINATES: protein motif:HMM:NF015659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HOH17897.1"
                     /translation="MKKNLIVLFVSILALLTLFFTIDTKINAAEPTAAGKVVFHYQLW
                     DGDYSSSGLWTWGTGTGGTSSAVTSTVTDAFGGVYEINVMSDADDEIGLIPLRKDITL
                     DSRWDSRETPDGYHIKFNVTALKNGTVNELHVYYFQGGYQTYYLADPAKVNVIVLYYD
                     PSGTYEDNLGVHAWNGWENFTEPSWGTPAKVFSDGFKSPGNVQGKAALLQFTPDGTKD
                     PGFLIYAGSDQTKKHASAGDIKGFKDLKAGAVKVVYVSGGSVYDGVDKRQTFVDNAFT
                     FDFIPFALATLDGTYAKDPRVIFVKTSIAVTTKEKTGEETYYVNEVIGQKIEVVPSED
                     GWKLPTDLPEYPAYKATALPANVKGRVVFHYQKWDGNYTGTGLWTWNAGTDGTKDGVA
                     MTGVDAFGAVMEINIDDDAADTIGIIPIGAEIGSDARWNHRETPDGQHINFDVTAIKA
                     GTATTIHVYYFQGGFQTYYVADPAKANVLVLYFDKTGTYEENLGVHAWNGWENFTETD
                     WGTPAKVFKDGFKSPDNVQGKAALLQFTPDGTKDPGLLIYAGDDATKKHPSGNLDGFK
                     TMTAGQVKLVYVTVETIFQSRIDFAPVAFGGEVIFTDVYGDVAYVRDVMGLIDLKTYF
                     TLKQGTTVVPIAQIDYNTTADSTNEFVIQLAEANALNNTKAYTLHFNNGLTGDKQLLA
                     EIALDLDKEKPVIALVDPDAAITVALGAKWDQSLFPVYRVTDNRDANLTPKVYVAKGH
                     GTLDTNKVGDYPIRLEVVDEWGNVGQLDFTITVVAPEKGCKANSASAMVGFGLLGLVF
                     FFRRRRYA"
     gene            3044..>3448
                     /locus_tag="PK087_01105"
     CDS             3044..>3448
                     /locus_tag="PK087_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HOH17898.1"
                     /translation="MKKRLLMMFLLLLLIIPISIAAAETNVSTLKIHYYRYGNDYDRG
                     WNIWLWPFGSEGEQIDFDKNEGVFVQDDWGVVATVDLSATKYRNITQIGIIFRRGAWA
                     EKDIDFDRYIQIPTTAENGLLNVYFVEGDERMG"
BASE COUNT         1001 a          767 c          656 g         1024 t
ORIGIN      
        1 ttgagccata tggtcatgcg ggaattggaa gacatcggca cctttaccgg atttaccata
       61 ggtttttaat ttttcgcgaa tatcaaccgt tcccatgtgt tggaattcaa ccttaatgcc
      121 cggatttaat ttttcaaaag ccgggatgat ttcatccata aaaacacctt cactgtcatc
      181 catccacaca cgaatgactt gagctttaac ttgggtttca tcaaacttga ttacttctcc
      241 ggttgtcgcg gtggtttgtt ccgagcaacc gacaatcatt aacattaaaa caaatacgga
      301 aatgatgctt aataattttt tcattttctt tctcctttcc gcttacgcat atcttcttct
      361 tctaaagaag aaaactaatc ctaataatcc gaacccgacc atagcactag cactgttggc
      421 tttgcagcct ttttccggtg caacaacggt aatcgtaaaa tctaattgcc caacattacc
      481 ccattcatca acaacttcca atctaattgg gtaatcgccg actttattcg tatctagggt
      541 tccatgtccc ttcgccacat aaactttcgg tgtaaggtta gcatcgcgat tgtccgtaac
      601 gcgataaacc gggaagaggg attgatccca tttagcgccc aaagcaaccg taatcgcggc
      661 atccggatca actaaagcaa tcaccggttt ttctttatct aagtctaaag caatctcagc
      721 taataattgt ttatcgcccg ttaaaccgtt gttgaagtgc aaggtataag ctttggtatt
      781 gtttaaggca ttggcttccg ctaactgaat aacgaattca ttagttgaat cagcggtcgt
      841 gttatagtca atttgcgcga ttggcacaac tgttgttcct tgttttaaag taaagtaggt
      901 tttcaaatca attaatccca taacatcacg aacatatgcc acatcaccat aaacatcggt
      961 gaaaattact tcgccaccaa aagcaacggg tgcaaagtca attcgggatt ggaaaatcgt
     1021 ttccacggtg acatagacaa gtttaacttg gccagccgtc atggttttga aaccatcaag
     1081 attaccggaa ggatgtttct tggtagcatc atcgcccgca taaattagta atcccggatc
     1141 tttagtgcca tcaggtgtga attgcaataa ggcggctttg ccttggacat tgtccggtga
     1201 cttaaagcca tccttaaaca ccttagccgg tgttccccaa tcggtttccg taaagttttc
     1261 ccagccattc caagcatgaa cgccgaggtt ttcttcatag gtgccggtct tatcaaagta
     1321 taaaaccaaa acattggctt tagccggatc ggcaacatag taggtttgga atccgccttg
     1381 gaaatagtag acatgaatag tggtcgcagt tcccgcttta atggcggtaa catcaaaatt
     1441 gatgtgttgg ccatccgggg tttcccgatg attccaacga gcatcactac caatttcggc
     1501 accaatcgga ataataccga tggtatccgc agcatcatcg tcaatgttga tttccataac
     1561 ggcgccgaaa gcatcgactc ccgtcatcgc aactccatct ttagtgccgt cagtcccggc
     1621 attccaagtc catagtcctg ttcccgtata gtttccatcc catttttggt aatggaaaac
     1681 aacgcgccct tttacatttg caggaagagc ggtcgcctta taagcgggat attccggtaa
     1741 atccgtcggt aatttccagc cgtcttcact cggaacaact tcaatttttt gaccgattac
     1801 ttcgttaaca taataagttt cttcaccggt tttttctttc gtagtcaccg caattgatgt
     1861 tttaacgaaa ataacccgtg gatctttcgc ataagttcca tcaagagttg ctaaagcaaa
     1921 aggaataaag tcaaaggtaa aggcgttatc aacaaatgtt tgacgcttgt caacgccatc
     1981 ataaacactg ccgcccgaaa cataaacgac tttgacggct ccggctttta aatctttgaa
     2041 acctttaata tcaccggccg aagcatgttt cttcgtttga tcactgccgg cataaattaa
     2101 aaagcctgga tctttcgtgc catcgggagt aaattgcaat aaggccgctt tgccttgaac
     2161 attacccggt gacttaaagc catccgaaaa caccttagcg ggagttcccc aacttggttc
     2221 cgtaaagttt tcccatccgt tccatgcatg aacgcctaag ttatcttcat atgttcccga
     2281 tggatcatag taaagaacga taacattgac tttagcagga tcagcaagat agtaagtttg
     2341 gtatccgcct tggaaataat aaacatggag ttcattaacg gttccattct ttaaagcagt
     2401 cacattaaac ttaatgtgat agccatccgg tgtttcccga gaatcccaac gcgaatccaa
     2461 agtaatgtct ttccgaaggg gaattaagcc aatttcatca tcagcgtcgg acataacatt
     2521 aatttcataa acaccaccaa aagcatccgt aacagtactt gttacggcac tactggttcc
     2581 tcccgttccg gttccccatg tccataaacc ggagcttgaa taatccccat cccataattg
     2641 gtaatggaag accacttttc cagctgccgt cggttcagca gcattaattt tcgtatcaat
     2701 tgtgaaaaag agcgttaata aagcaaggat tgatacgaaa agtacgatca gatttttctt
     2761 cattttttcc tccttgaaaa aaagataaaa gagagtctta cataaacttc tcatacataa
     2821 ttattttaac actttcttgt ttttttgcaa gcgatttcat ttatgtaatg gttttcataa
     2881 tcaagaagtt tttctttcac ttcttttcgt ttaagaaaac cgttcttttt aatgcaaacg
     2941 atttcattta tggaaaccat tacacatttt aggcaattga aaatcttaac atcttatagt
     3001 aaaataaact aaagcaacaa acaagttttg ggaaaggatt actatgaaaa aaagattatt
     3061 aatgatgttt ttactgctgc ttttgataat ccccatcagc atcgccgccg ccgaaactaa
     3121 tgtttccacc ctcaaaatcc attattatcg ctatggaaac gattatgata ggggttggaa
     3181 tatctggctt tggccctttg gaagcgaagg tgagcagatt gattttgata aaaacgaggg
     3241 tgtatttgta caagatgact ggggagtggt ggcaaccgtt gacttaagcg ccaccaagta
     3301 tcgcaacatc acccaaatcg gtatcatttt tcgcaggggt gcttgggcgg aaaaagatat
     3361 tgatttcgac cgctatattc aaataccgac gacagccgaa aacggccttt taaatgttta
     3421 ttttgtggaa ggagatgaaa gaatgggg
//
LOCUS       DAOSGN010000042         3387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_26110_length_3387_cov_2.911164, whole genome shotgun sequence.
ACCESSION   DAOSGN010000042 DAOSGN010000000
VERSION     DAOSGN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26110_length_3387_cov_2.911164"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..633
                     /locus_tag="PK087_01110"
     CDS             130..633
                     /locus_tag="PK087_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014644057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HOH17899.1"
                     /translation="MRKCYVGSQNRVKLLAVKRVLKNYEVIGFEVNSQVSKQPKSDLE
                     TIQGAVNRALCLPKDGLRIGLEAGVQLHEERLFLVNWGVLIDEYEHQYMAGGTRIPLP
                     DFMKEALLEQGRELAEVMDEYTHSSNIRQKQGAIGVFTNNLVKREDIFIHIVKLLYGQ
                     YKRGENE"
     gene            630..1205
                     /locus_tag="PK087_01115"
     CDS             630..1205
                     /locus_tag="PK087_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOH17900.1"
                     /translation="MKGLIILADGFEDVEAIATIDVLRRSKINVDVVTINKNLFVESA
                     HNIVVKAEKEWKKVTEKDYDFLVIPGGRAVYSVWDQFAPLTNLINQFVSSQKLVAAIC
                     AGPALVGKLGHYHNHSYTCFPGSEKTIVGGQYLPKESVVRDGNFITAKAMAFTFEFSL
                     AIIEFLQGKEQKEQVRRNIRGELRETISQFF"
     gene            1303..2745
                     /locus_tag="PK087_01120"
     CDS             1303..2745
                     /locus_tag="PK087_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17901.1"
                     /translation="MNEKPIETPKISKPRKPRTTAAKTVPIEEIDVQKLNSIPEKTPT
                     LSPIEELTQKKIVNLPEEPPKPTEEELFEAAISKKKSRKKDKKVEEKAIIRYLDTPIL
                     EGLSDEIVAQRKADGLINVAKKTQGKSIFGIIVSNLFTYFNIVYMIITVLFVVLNSTD
                     NLLFLATIIPNLIIGIAQEIKAKLTVDKLSLLSAPTTTVVRNGEKLEIPISEIVLDDI
                     VFYTPGKQISSDSIVVDGTLEVNESLLTGESEAVIKNVGSPLLSGSFVVSGVAVARVD
                     KVGKDNYIEKLSADARLYRKPKSELLTTLNWIIRIVSFIILPLAVLTYMTTSKDAIKD
                     FLYLGTWAEKMFGRQPGYFLNRDGVIKAGSSILAMIPAGLFLLTSMALAVGVIRLGKS
                     KTLVQELYCIEMLARINVLCLDKTGTITDGTMRVCDCVEVKNYTDYTIREIVGSMMNA
                     FTDVNPTSEALIRFFDKNKVLAPTAIIPFS"
     gene            2815..>3387
                     /locus_tag="PK087_01125"
     CDS             2815..>3387
                     /locus_tag="PK087_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOH17902.1"
                     /translation="MFDNYDKVQSKVERFAQQGCRVLALGHTTLKVKENDIPKTIKPL
                     ALIVIQDHIRDDAADTIAYFKHNGVDVKVISGDNPITVSEIALRAGVENAERFISLAG
                     LTDDQVRESVFEYTVFGRVSPVQKRIIIQTLKKHKKVVAMTGDGVNDILALKEADCSI
                     AMASGSEATRYVSHLVLMDSNFSSMPKVVDE"
BASE COUNT         1043 a          562 c          762 g         1020 t
ORIGIN      
        1 ttgggcttcc gggccgctat atccattcca atgccaccat ggttcatgtt gatgatatgt
       61 tagcggttaa aaaaatggtt ttagctgtcg ttaaagattt cgattctaaa aggcttatgg
      121 ctttacataa tgcgtaaatg ttatgtaggc tcacaaaatc gagtcaaact tttagctgtt
      181 aaaagagttt taaaaaatta tgaagttatc gggtttgagg ttaatagcca ggtttccaaa
      241 caaccgaaat ccgatttgga aaccattcaa ggggctgtta atcgagcttt atgtttaccg
      301 aaagatggct taagaatcgg tttggaagcc ggagtccagc ttcatgaaga gcgtttgttt
      361 ttagttaatt ggggcgtttt aattgatgaa tatgagcatc aatacatggc cgggggtaca
      421 agaatccctc tgccggattt tatgaaagaa gcattgttgg aacaaggaag ggaacttgcg
      481 gaagtcatgg atgaatacac tcattcaagt aatatccgcc aaaagcaagg ggcaattggt
      541 gtcttcacca ataatttggt aaaacgggaa gatattttta tacatattgt gaaattactt
      601 tatggacaat ataagcgagg tgagaatgaa tgaaaggtct gataatttta gctgatggct
      661 ttgaggatgt cgaagccatc gctacgattg atgttttgcg tcggagtaaa atcaatgttg
      721 atgtggtcac gattaataag aatttatttg tggaatcagc gcataacatt gttgttaaag
      781 cggaaaaaga atggaaaaaa gttacggaga aagattatga ttttttagtt attcccggtg
      841 gaagagcggt ttattccgta tgggatcaat ttgctccctt aacaaacttg attaatcaat
      901 ttgtgtcatc ccaaaaatta gtggcggcca tttgtgcggg tccggcttta gttggtaaac
      961 tgggacatta ccataatcat tcctatactt gttttcccgg cagtgaaaaa accattgttg
     1021 gcgggcaata tttgccgaag gaaagcgttg ttcgggatgg aaattttatt actgcgaaag
     1081 cgatggcttt tacttttgaa tttagtttag ccattatcga gttccttcaa ggtaaggagc
     1141 aaaaagaaca agtacggcgg aatattcgtg gtgaactgag agaaacaatc tcacagtttt
     1201 tttaaatgat ttataaaaat aaaatgttct tgtttattag caggtgttat ggtaaaatta
     1261 gactatatta tttaccaaaa gtagagaaaa gtggtgacaa aaatgaacga aaagccaatt
     1321 gaaacgccca aaatttctaa accgcggaaa cctcgaacaa cagctgcgaa aacggtgcca
     1381 attgaagaaa tcgatgtgca gaaattaaat tccattccgg aaaaaacacc tactttatca
     1441 ccgattgaag aattaacaca aaagaaaatt gttaatctac cggaagaacc gcccaaaccg
     1501 acggaagaag aattgtttga agcggcgatt agtaaaaaga aaagcagaaa aaaagacaaa
     1561 aaagttgaag aaaaagccat tatccgctat cttgatactc ctattttaga aggtttaagc
     1621 gacgaaattg ttgctcaaag aaaagcggat ggattgatta atgtcgcaaa gaaaacccaa
     1681 ggaaagtcca ttttcgggat tattgtttcc aatcttttca catacttcaa cattgtgtat
     1741 atgattatta cggttttgtt tgttgtttta aatagtacgg ataatttatt attcttagcg
     1801 acgattattc ctaatttaat cattgggatt gcgcaagaaa ttaaagccaa attaacagtt
     1861 gataaattgt cgcttttgag tgcgcccacc acgacggtgg ttaggaacgg tgagaagttg
     1921 gaaatcccaa tttcggaaat tgtcttggat gatatcgtct tctatacacc gggcaagcaa
     1981 ataagctccg acagtattgt tgtggatgga actttggaag tcaatgaatc cttattaacc
     2041 ggtgaatcgg aagccgtgat taaaaatgtc ggcagtccgc ttttgtcggg tagttttgtg
     2101 gtcagcgggg ttgcggtggc acgtgtcgac aaagtcggga aagataatta cattgaaaaa
     2161 ttatctgcgg atgcgcgttt gtatcgcaaa ccgaaatcgg aacttttaac aaccctaaac
     2221 tggattattc ggattgtttc ttttattatt ttacccttag ccgttcttac ttatatgacc
     2281 acatcaaaag atgctattaa ggactttttg tatttaggaa cttgggcgga aaaaatgttt
     2341 gggcgtcaac ccggatattt cttaaaccgt gatggtgtta ttaaagccgg aagttcgatt
     2401 ctagcgatga ttccggcggg tttgttctta ttaaccagta tggccttagc ggtcggggtt
     2461 attcgtttag gaaaaagcaa aaccttagtt caggaattat actgcattga aatgctggcc
     2521 cgcattaatg ttctttgctt ggacaaaaca gggaccatta ccgatggcac aatgcgcgtt
     2581 tgtgattgcg tggaagttaa aaactacacc gattacacga ttcgggaaat tgtcggttca
     2641 atgatgaatg cctttaccga tgtcaatccg acttcggaag ccttaattcg tttttttgat
     2701 aaaaataaag ttttggcacc gacagccatt attccttttt cttgaaaacg gaaattcagt
     2761 gcggtaactt ttggccgcca aggaacgttt tttctgggtg ctccggaatt catcttgttt
     2821 gacaattatg ataaagttca aagcaaagtg gaacgctttg ctcagcaagg atgtcgggtt
     2881 ttagcgctgg gacatacgac tttaaaagtt aaagaaaatg atattcctaa aaccattaaa
     2941 cccttagccc tcattgtcat tcaagaccat attcgtgatg atgccgctga tacaattgct
     3001 tacttcaaac ataacggtgt ggatgttaaa gttatttccg gtgataatcc gattacggtt
     3061 tccgaaatcg ctttacgggc cggtgtcgaa aatgcggaac gctttattag tttagcggga
     3121 ttgaccgatg atcaggttcg ggaatcggtt tttgaatata cggtatttgg tcgggtcagc
     3181 cccgttcaaa aacgcattat tattcaaacc ttgaaaaaac ataaaaaagt cgtggcgatg
     3241 accggtgacg gtgtcaacga cattttagca ctcaaagagg ccgattgcag tattgcgatg
     3301 gcttccggaa gtgaagcgac tagatatgtc tctcatttag ttttaatgga ttccaatttt
     3361 tcatcgatgc cgaaagttgt tgatgaa
//
LOCUS       DAOSGN010000043         3387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_26111_length_3387_cov_2.636555, whole genome shotgun sequence.
ACCESSION   DAOSGN010000043 DAOSGN010000000
VERSION     DAOSGN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26111_length_3387_cov_2.636555"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PK087_01130"
     CDS             <1..720
                     /locus_tag="PK087_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH17903.1"
                     /translation="QKVINEINVGDVVLRTHMIGADKYVKENSGYLQKYEEHRDYNVF
                     FSKSYQIDYNPEAILDEYYQFGKKCRNKKITIFDVAGNQGGNSRFPQSFIKGLNEYAY
                     WQSGCAVLVNPVFNPQSKGKSYQIESTPQEYDNNKGQYKGTLYVIMNKGTTSSGEAAV
                     SYAKSCPNSIFLGSATGGIGTFGDVKHYRLRNSKLLFQFGHKIFYMPNYEEGKGFIPN
                     YWLDSKNPWLYLKRYLKHSKN"
     gene            1000..2712
                     /locus_tag="PK087_01135"
     CDS             1000..2712
                     /locus_tag="PK087_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17904.1"
                     /translation="MEYFSTFNFDIIIVLVLLASLITGAYYSSFRQGRKTLMLIVPLV
                     ALYFVLPPLMKFIKSTSAVDNLLIKIVTFIGRYLKLSAYHNVMMTGLVALVAFIVMSL
                     IIAFIYNLFAQSMEKQVLTSPTKFSRTLAALLGLINGYVLVILLMLLIKPVADINYHA
                     PLSKLIGDTSTAYLPVSKLNEVQNINPTLHQEYQEAYDFISGNEVQNTLDYFVSLNDE
                     FTDINTYIDTIMFNQLSTDSKALITAHLSGNDYVTALLTEVSGTLVLNTVLTKEKNHP
                     EMTTIREKLSYLNDYRAYWTLFSTLLTDPIASYDYQEIATIYLNNQETLLSLFSQLRL
                     RNDFIQKMNVLSLFAHYYPAFKTILNDNAAIDFTSYRTRFNLAMSNNLYKYAQAVVTY
                     AFPERDNVVISLQTLFTEVLKQEPKMVLLDKNMGIPTQVILAKRYDEWFTTPLWETEV
                     LINSYLLDSLGSHQTGGYPLYHEYFFFQYLSRGVTWDNQFSADDFVIMLNNLAGTVTN
                     GLITSAQASAYLDGLLAQPQSVIRTLEQQGKMTATFYEELSLINHSLFSENWPRLLEV
                     LAGE"
     gene            2714..>3387
                     /locus_tag="PK087_01140"
     CDS             2714..>3387
                     /locus_tag="PK087_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17905.1"
                     /translation="MAKKRKETIVEYVLTTKGYNKVVAGKNDHEVGVHPSQRQINNLD
                     TAKTQFLRTNPFMYNSKTSAYPITSIEEYLVKVRKSEFALGDIVRNPDLSKTKKRRLV
                     KRAFKNWNKDYNTQKSRAFSDSDKTMEVIGGVNFMKISFATKLVMMVLFLLMIFIVSL
                     NSYLWMKIAQTNFGFRLRQNVMDLFNTTAWLKMLANLTIYVLVMFMFYATIYSMVSKD
                     FVRNHK"
BASE COUNT         1031 a          596 c          649 g         1111 t
ORIGIN      
        1 caaaaagtaa ttaacgaaat taatgttggt gatgttgttc ttcgtactca tatgattggt
       61 gcggacaaat atgtaaagga aaatagtggc tatcttcaaa agtatgaaga acatcgagat
      121 tataatgttt ttttcagtaa gtcttatcag attgattata atccggaagc aatattagat
      181 gaatactatc agtttgggaa aaaatgtcga aataaaaaaa taactatttt cgatgttgcc
      241 gggaatcaag gcggtaattc taggtttccc caaagcttta ttaaagggtt aaatgagtat
      301 gcctattggc aatcaggatg tgccgtttta gtcaatcccg tctttaatcc tcaatccaaa
      361 ggaaaaagtt atcaaattga aagcactcct caagaatatg acaataataa aggtcaatat
      421 aagggcactt tatatgtcat catgaacaag gggacgacat cgtcgggcga agctgccgtt
      481 tcatatgcaa aaagttgccc caatagtatc tttcttggtt ctgcaacggg cgggattggc
      541 acctttggtg atgtcaaaca ctatcgatta aggaattcaa aactcttatt tcaatttggt
      601 cataaaatct tttacatgcc taattatgaa gaaggtaaag ggtttatccc taattattgg
      661 cttgattcca aaaatccttg gctttattta aagcggtatt tgaaacatag caaaaattag
      721 cgatttttaa tggcgctgtt aaataacttg gggtttttaa gtttaggaaa aaaactagga
      781 atttttaatc tcattatttt agtagttaaa taaacaatgg ttcttatcat tgaaggcatg
      841 atgcattcat ccattatcag cagttttttg attaccaata atcgcgctgc ttttttgttt
      901 tttaaattaa aggtgatatt tgtaaaatta tttgtattac aaccgataaa atgttataat
      961 aagtaaaata acaaggatgg aatctgcgaa ggagttcgtg tggaatactt ctcaacgttt
     1021 aattttgata ttattattgt actggtgttg cttgcttccc ttattaccgg tgcttattat
     1081 agttcattcc gtcaaggacg aaaaacattg atgttgattg ttccgttagt ggctttatat
     1141 tttgttttac caccactgat gaagttcata aaatcgactt ctgcggttga caatctctta
     1201 attaaaatcg ttacctttat tggtcgctat ttaaaattaa gtgcctacca taatgtgatg
     1261 atgaccggtt tagtggcttt ggtggccttc atcgtaatga gcttgattat tgctttcatt
     1321 tataacctct ttgctcaatc gatggaaaaa caggtcttaa catcaccaac gaagttttct
     1381 cgcactttag ccgctttgct cggcctcatc aacggttatg ttttggttat tttattgatg
     1441 ttgttgatta agccggtcgc ggatatcaat tatcacgctc ccctttcgaa actcatcggg
     1501 gataccagta cagcttactt accggtttcc aaactgaatg aagtgcaaaa cattaatcca
     1561 accttgcatc aggaatatca ggaagcctat gattttatca gtggcaacga agtacaaaac
     1621 actttagact attttgtttc gcttaacgat gaatttaccg acatcaatac ttatattgac
     1681 acgataatgt ttaatcagct ttccactgat tcaaaagctt tgattacggc ccacttaagc
     1741 ggtaacgatt atgtgactgc gcttttgacc gaagttagtg gaaccttagt tttaaatacg
     1801 gtgttaacga aagaaaaaaa tcatccggaa atgacgacga ttcgtgagaa attaagctac
     1861 cttaacgatt atcgtgctta ttggacctta ttttcaacac ttttgactga cccaattgct
     1921 agctatgact accaagaaat tgcgacgatt taccttaata atcaagagac attattgtcc
     1981 ttattttccc aactgcgact tcgcaatgat ttcattcaaa aaatgaatgt cttaagtttg
     2041 tttgcccatt attatccggc ttttaaaacg attctaaacg ataatgccgc catagatttt
     2101 acaagttacc gcactcgttt taaccttgcc atgagtaata atttgtataa atatgcccaa
     2161 gcggtggtga cttatgcttt tcctgaacgt gataatgtcg ttatttcgct gcagacttta
     2221 tttactgaag tcttaaagca agaaccgaaa atggttttac tggataagaa tatgggcatc
     2281 ccaacccaag tcatcttggc taaacgctat gatgaatggt ttacgacccc gctttgggaa
     2341 accgaagttt taattaatag ttatttgctg gacagtctcg gaagccatca aaccggtggt
     2401 tatcctcttt accatgaata tttctttttt caatatttga gtagaggtgt aacttgggat
     2461 aatcaattca gtgccgatga ttttgttatc atgttaaata atttagcagg gactgttacc
     2521 aatggtttaa tcacttccgc ccaagccagc gcttatttgg atggcttgtt ggcgcagccc
     2581 caaagtgtca tccgcacttt agagcaacaa gggaaaatga ccgctacttt ctatgaagaa
     2641 ctttctctta taaatcactc tttgtttagc gaaaattggc caaggctttt ggaagttttg
     2701 gcgggtgaat aagatggcta aaaagagaaa agaaacaatt gtggaatatg tgttaacgac
     2761 gaagggttat aacaaagtag ttgcgggcaa aaacgaccac gaggtgggag ttcatccgtc
     2821 ccaaaggcaa atcaataatc ttgatacggc caaaacccaa ttcttacgca ctaatccctt
     2881 tatgtataat tccaagactt cggcttatcc gattaccagt attgaagaat atctggtgaa
     2941 agttcgtaaa agtgaattcg ctttaggcga tatcgttcgt aatccggatc tttccaaaac
     3001 taagaaacgc cgcttggtga aaagagcctt taaaaattgg aacaaagatt ataatacgca
     3061 aaaaagccgg gcttttagtg acagcgataa aaccatggaa gtcatcggtg gtgtcaattt
     3121 tatgaagatt tcctttgcta cgaagctggt gatgatggtg ttgttcttgt tgatgatttt
     3181 tatcgtttcc cttaattcct atttatggat gaaaattgcc caaacaaatt tcgggtttcg
     3241 gttgcgccaa aatgtaatgg atttgttcaa taccaccgcc tggttgaaaa tgcttgcgaa
     3301 ccttaccatc tatgttctgg tgatgtttat gttctacgcc accatctaca gtatggtttc
     3361 gaaggatttt gttcgaaacc ataaaat
//
LOCUS       DAOSGN010000044         3361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_26407_length_3361_cov_2.323049, whole genome shotgun sequence.
ACCESSION   DAOSGN010000044 DAOSGN010000000
VERSION     DAOSGN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26407_length_3361_cov_2.323049"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1084
                     /locus_tag="PK087_01145"
     CDS             167..1084
                     /locus_tag="PK087_01145"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOH17906.1"
                     /translation="MKAQALVSVIGLSGQSLFFQVDHFHQAGETLISECLFVEPGGKG
                     FNQAVALSLLGLKVAFLSAVGNDEYGHQCEQYLVKRGIKAHIVYKQNQQTAIASILRN
                     RDGDNQVTVFPGASLSKDDVDHFTSSLASSKALLLQLEVADEVLRVAVNMASKKHQLI
                     VLNPAPYRVIPEDILHKVDILTPNESEARQMAGLDFSSDWGLVVKKLKQLKKRIVVTL
                     GAKGAYYLDNHEEGFIPAFKVACVDSTGAGDAFNAALVRELLHKKTLKEAVRWANAVA
                     AFSVTKPHVLNSYPTSAEVEQFLKEQNDE"
     gene            1077..2027
                     /locus_tag="PK087_01150"
     CDS             1077..2027
                     /locus_tag="PK087_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17907.1"
                     /translation="MSDLQILYQKIANLLDEIDFSALWPDFHPFPFALYNQEEVVIDN
                     EKRPITPQFIGNTAIKWEESWLAIWNIQDASDNDYQLLIVKLVHEMFHAFQYEQKEKR
                     FPNEMDGLRYGFSALNLTIKYQENLLLGELSRHFTNTKWDEFIKRRNYRALHFPKQVD
                     YESKIEVVEGMAQFVEMQALKQIDEQVFIEEHNKLLLRLLDQNRLLKIRHLSYDVGTM
                     LLLIMDAQKCSFKHRLGEETKTIFAMLSQPSADVNFDFKIDEQIESLLKNDFGKKQAQ
                     ITKVLETGKSVNKDFKIIGFDPLNAFIIDPYLYNPHFVMI"
     gene            2234..>3361
                     /locus_tag="PK087_01155"
     CDS             2234..>3361
                     /locus_tag="PK087_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOH17908.1"
                     /translation="MLIITGNDLTLHDFMKVARKKEAVTLSSEAKTKINQSNQWIRAL
                     VKSNKTVYGVNTGFGKMSDVIISKDNLADLQIKLLKSHACGVGKHFSEEIVRGMMLLR
                     VNALAKGYSGIRLVVLEQIIAYLNHDIIPAIPEQGSLGASGDLAQLSHMGLTLVGLGE
                     VYYQKRLMPTQEALALAELKPLAGLQAKEGLSLINGTQAMTAVGAIVLYDAIKLAKLA
                     DIASALSLEALLGVKEAFDERIHQVRGQNGQIKVAENIHFLCRNSTYLSSQGEIRVQD
                     AYSLRCIAQVHGASRDVLEYVKSKVEIEMNAATDNPLIFGPDTVISGGNFHGQPLALP
                     FDFLKVALCELGSISERRIERLVNPSLNGGLPAFLASSPGLN"
BASE COUNT         1027 a          612 c          717 g         1005 t
ORIGIN      
        1 aaacccggcg aagaataccg agaatcctat gttgtggaaa cattgcggcg tttaggcttt
       61 gatccgactg ccgatattga ataatcttta cctccctcct tggggaggtt tttttacttg
      121 tcatttagtt tctttttttg gtagaatata agagaggtat tttaatatga aagcacaagc
      181 ccttgttagt gttattggct taagtggcca atcgcttttt ttccaagttg atcattttca
      241 tcaagccggt gaaactttaa tcagtgaatg tttatttgtg gagccggggg gaaaaggttt
      301 taaccaagcg gtagctttgt ccttattagg ccttaaagtc gcttttttat cagcggttgg
      361 aaatgatgaa tatgggcacc aatgtgaaca atatttagta aaaaggggca ttaaagctca
      421 tatcgtttac aaacaaaacc aacaaacggc catcgcaagc attctaagaa atcgggatgg
      481 tgataatcaa gtaacggttt ttccgggagc atccttaagc aaagacgatg ttgaccattt
      541 tacctcatcg ctggcttctt caaaagcgct cttacttcag cttgaggtcg ccgatgaagt
      601 tttgcgagta gccgtcaata tggctagtaa aaaacaccaa ttaatcgttt taaatcccgc
      661 tccttaccga gtcattcccg aagatattct tcataaagtc gatattttaa cacctaatga
      721 aagtgaagcc cgccaaatgg ccggtttaga cttttctagt gactggggct tggttgtaaa
      781 aaaattaaaa caattaaaaa aacgaattgt ggtaactctc ggggccaagg gcgcttacta
      841 ccttgataat catgaagaag gctttattcc ggcctttaaa gttgcctgtg ttgattcaac
      901 cggcgccggg gatgctttta atgctgcttt agttagagaa ttactacata aaaaaacctt
      961 aaaagaagcg gtgcgctggg ccaatgccgt cgccgctttc agtgtcacga agccccatgt
     1021 gcttaattcc tatccgacca gtgcggaagt cgaacaattt ttaaaggagc aaaacgatga
     1081 gtgatttaca aattctttac caaaaaattg ctaacttact tgatgaaatt gatttttccg
     1141 ccctgtggcc ggattttcac cctttcccct ttgctttgta taatcaagaa gaagtagtca
     1201 tcgataatga aaaaagaccc ataaccccac aatttatcgg caacaccgcc atcaaatggg
     1261 aggaatcttg gttagctatt tggaatattc aagacgcaag tgataatgat tatcaattgt
     1321 tgatcgttaa actggtgcat gagatgttcc atgcttttca atatgagcag aaggaaaaac
     1381 gctttcccaa tgaaatggat ggtcttcgtt atggtttttc ggccttgaat ttaacgatta
     1441 agtatcaaga aaacctctta cttggagaat taagtcgcca ttttacaaac acaaagtggg
     1501 atgaatttat taaacggcgt aattaccgag cgcttcactt tcccaaacaa gtggactatg
     1561 aaagcaagat agaggttgtg gaagggatgg cccagtttgt ggaaatgcaa gccctaaaac
     1621 aaattgatga acaagttttt atagaagagc ataataagtt attgctgcgc ttacttgatc
     1681 aaaatcgctt gttgaaaatt agacatctat cctatgatgt gggaacaatg cttttattaa
     1741 taatggatgc tcaaaaatgc tcatttaagc accggcttgg tgaggaaacg aaaacgattt
     1801 tcgcaatgct ctcccagccc agcgctgatg taaacttcga ttttaaaatt gatgaacaaa
     1861 ttgaaagttt gttaaagaat gattttggaa aaaaacaagc acaaataact aaagttttgg
     1921 aaacagggaa aagcgtaaac aaagatttta aaattatagg ttttgatccc ttgaatgctt
     1981 ttattattga cccctatctc tataatcccc attttgtgat gatttaaact gccggtcaaa
     2041 gggaaatcat taaacaaacg gtcgtttgtg aattaagtgc tgatttccaa ggtaaaaccc
     2101 tctacttgtc aggaaattta aattaaggga agaaaagatt ttaataaatt ttttcttctt
     2161 ttttcttagt aatcccatct tgctataatt aggcttttat tgtaaaataa gaatgaataa
     2221 agaggagact tttatgttaa taattacggg gaatgattta acactgcatg actttatgaa
     2281 ggttgctaga aaaaaagaag cggtcacctt atcaagcgaa gctaaaacca agattaatca
     2341 atcaaaccaa tggattagag cgcttgtgaa aagcaataaa acggtttatg gagttaatac
     2401 gggttttggg aaaatgagtg atgtcatcat ttccaaagac aatctcgccg atttacagat
     2461 taaattgtta aagtcccatg cttgtggcgt tggcaaacat ttttccgaag aaatcgtccg
     2521 tggcatgatg ttgttacgcg ttaatgccct tgccaagggt tattccggaa ttcgtttagt
     2581 ggtcttagag caaattattg cttatttgaa ccatgatatt attcctgcga ttcccgaaca
     2641 aggctcattg ggagcgagtg gcgatttagc ccagttatca catatgggtt tgacgcttgt
     2701 tggtttgggg gaagtttatt atcaaaagcg gctcatgcca acacaagaag ctttagcttt
     2761 ggccgagtta aaacctttgg ccggtttaca ggctaaagag ggtttatcgt taatcaacgg
     2821 cacccaagcc atgacggcgg tgggagccat tgtcctctat gatgccatta aactagcaaa
     2881 attagcggat attgcgagtg ccttatcact agaagctctt ttaggtgtta aagaagcttt
     2941 tgatgagcgg attcatcaag ttagaggaca aaacggtcaa atcaaggtag cggaaaatat
     3001 ccatttttta tgccgtaaca gtacttattt aagcagtcag ggcgaaattc gggttcaaga
     3061 tgcttattca cttcgctgta ttgcccaagt tcatggggcg agccgtgatg tactggaata
     3121 tgtgaaaagc aaagtggaaa tagaaatgaa tgcggcgacg gataatccgc ttatttttgg
     3181 gcccgacacc gttatttccg gaggcaattt tcatggtcaa ccactagccc tcccctttga
     3241 cttcttaaaa gtggcccttt gtgaactagg aagtattagt gaacgccgca ttgaaagatt
     3301 ggtcaatccc agtttaaacg gcggtctacc ggccttttta gcaagtagcc ccggtttaaa
     3361 t
//
LOCUS       DAOSGN010000045         3324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_26829_length_3324_cov_4.042827, whole genome shotgun sequence.
ACCESSION   DAOSGN010000045 DAOSGN010000000
VERSION     DAOSGN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26829_length_3324_cov_4.042827"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK087_01160"
     CDS             <1..186
                     /locus_tag="PK087_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17909.1"
                     /translation="VTALVFFKRDKVIFDPLWVLLADHSYIGPAIDRIVTQGSDFNEV
                     MDAQTNIYSARLLEVYG"
     gene            326..2770
                     /locus_tag="PK087_01165"
     CDS             326..2770
                     /locus_tag="PK087_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH17910.1"
                     /translation="MKKWITLTLVLLLAFVFVGCKPQEDEEPDVVNASSVEITGAKTE
                     IEVNEFLQLSATVKPAEAAQAVKWSSKNPAIATVDDNGKVKGIAAGKVEIVASAASNS
                     KIFKAYSLTVKAPVIVVNPTSVTLSGDIEVVKGQSITLVAAVLPAGVAQEVTFSSSDE
                     TIATVASDGKVSGLAEGKVTITVTVVADPTLKATYEITVKAPVVATSVTLTGSTNEVT
                     IGSNISLVATVLPEGAAQEVTYSSSDEAIATVTSKGKVNGIAEGTVTITVTVVAVPTL
                     TATYEVTVVKQEIPDVEKEPTDVIIEGEGQMQVGTQINLTSSVLPTGVNQNVTWKSSN
                     EKIATVSASGEVTAKAEGTVYITAASVANPDISAMHKIIVKPAPVVEPYPDLGGYQIK
                     IMAAGHAMHEHDPFLELYAASDKTAKQTAWLDVEKKFNCDLAVVAFPDVAPWGPARYN
                     WLNEKAALNDAETDIFVSTTDWLKVLADGGSIVDTTEYYNLYGKNQMAPAMKAASTYK
                     GGLFALPTVASAGLHVDSGIFYNVAFVERLGLTSPAKLFNEGKWTYTGFSNWVKEADA
                     LLAEDESVLSGRPFIYWQGMVNAGGVKLADTLTLTMNFKHAYAITAANVLKELYGLGW
                     GTNAWDAGATSFNEGKSLMQGGGLWFVRTDNRWPKTLWDAGGNSRFGYVPFPYPDGVA
                     KDNTRISSSGGACYMQAAGRPYPAGVTAKDIYRAFTEMMLQTTDLVKQDPTYSEEGIM
                     TTDAQKRIDDPESVTALVFFKRDKVIFDPLWGMLGVSTYIGPAIDRVVTQGADYNEVL
                     DAQTTTYQARLLELYS"
     gene            2882..>3324
                     /locus_tag="PK087_01170"
     CDS             2882..>3324
                     /locus_tag="PK087_01170"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HOH17911.1"
                     /translation="MNLDKMVNSKWYTGFEWGYRLVFLNLLMITIPALLGAIPFLLWR
                     RYPNAGWLVIVAIVLMLFGFIPCFIAAFRVIKAYREEQTGTLFGLFFTYLFNTFKNIY
                     LLELIMLPLFCLYTVAALFYWDIIGNYAQLDLPGWVAVISFVVLF"
BASE COUNT          916 a          635 c          784 g          989 t
ORIGIN      
        1 gttaccgctt tagtcttctt caaacgcgac aaagttattt ttgatccgct ttgggtttta
       61 ttggctgacc attcctacat cggtccggcc attgatcgga ttgttacgca aggttccgac
      121 tttaatgaag tcatggatgc gcaaaccaat atctattccg ctcgtttatt agaagtttat
      181 ggataaaaat ctgcggaaac atgtttaatt agattgcaaa aagagctctt tgatagtata
      241 atattatcgg agctcttttt ttaaaagata agagcaaaca ctcttttaag ccaagcttaa
      301 ataaaataat tctaaggagg aatttatgaa aaagtggata acattaacat tagtcttact
      361 tttggctttt gtttttgttg gttgtaaacc ccaagaagat gaagaacctg atgttgtcaa
      421 tgcctcatca gtcgagatta cgggagcgaa gacggaaatt gaagttaacg aattcttaca
      481 attgagcgcc accgtaaaac cggccgaagc tgcccaagct gttaagtggt catctaagaa
      541 tccggccatt gcgaccgttg atgataacgg taaagtaaaa ggaattgcgg ccggaaaagt
      601 ggagattgtt gcgtcagcag cttccaacag taaaattttc aaagcttatt cccttacggt
      661 taaagccccg gtcattgttg tcaatccgac ttcagtcacc ttaagcgggg atattgaagt
      721 cgttaagggt caaagcatta ctttagtggc cgctgtttta ccggctggag ttgctcaaga
      781 agttactttt tcttccagtg atgaaacgat tgcgaccgtt gccagtgatg gaaaagtaag
      841 tgggcttgcg gaagggaaag tcacgattac ggtgaccgtt gtcgcggatc cgactttaaa
      901 agcaacttat gaaattaccg tgaaagcccc agtggtggcg acatcggtaa ccttaactgg
      961 ttcgacgaat gaagtaacta tcggtagtaa cattagttta gtggccaccg ttttaccgga
     1021 aggtgccgct caagaagtca catattcttc tagtgatgaa gcgattgcga cggttacgag
     1081 caaaggaaaa gtgaacggga ttgcggaagg aacagttacc attactgtta cggtggttgc
     1141 cgttccgact ttaacggcta cttatgaagt cacagttgtg aaacaagaga ttccggatgt
     1201 ggaaaaagaa ccaaccgatg tcatcattga aggtgaaggc caaatgcaag ttggcacgca
     1261 aattaactta acatcgtcag ttctaccgac cggagttaac cagaatgtaa cttggaaaag
     1321 cagcaacgaa aagattgcga ctgttagtgc cagcggtgaa gttaccgcca aagccgaagg
     1381 aacggtttat attaccgccg cttcagttgc taatcccgat attagcgcta tgcacaagat
     1441 tattgtgaaa ccggctccgg tggttgaacc ctatcctgat ttaggtggtt atcaaattaa
     1501 gattatggcg gccggtcatg caatgcatga acatgatccg ttcttggaat tatatgccgc
     1561 ttccgataaa acggcaaaac aaacggcatg gttggatgtt gaaaagaaat tcaattgtga
     1621 tttagcagtt gtagcgttcc cggatgttgc tccgtggggt ccggcccgtt ataattggtt
     1681 aaatgaaaaa gcggctttaa atgatgctga aacggatatt tttgtttcca caaccgactg
     1741 gttaaaagta ttagccgatg gcggctcgat tgttgatacg accgaatact ataatttata
     1801 tggtaaaaac caaatggctc cggccatgaa agcggcttcc acttataaag gtggcttatt
     1861 tgcactacca acagtggctt cagccggttt acatgtcgat agtgggattt tctataatgt
     1921 tgctttcgtt gaacgtttag gcttaacttc accggcgaag ttatttaatg aaggcaagtg
     1981 gacctataca ggcttttcaa attgggttaa agaagctgat gccttattag ctgaagatga
     2041 aagtgtctta tcaggtcgtc cgttcattta ctggcaagga atggttaatg ccggtggtgt
     2101 taaactagcc gatactttaa ccttaacgat gaactttaaa catgcgtatg cgattaccgc
     2161 agccaatgtt ttgaaagaat tatatggtct tggttgggga accaatgctt gggatgccgg
     2221 cgctacttcc ttcaatgaag ggaaatcctt aatgcaaggc ggcggtcttt ggtttgtaag
     2281 aaccgataac cgttggccga aaactttatg ggatgccggt gggaattccc gcttcggcta
     2341 tgttcccttc ccgtatccgg atggggttgc gaaagacaat acgcggatat ccagttccgg
     2401 tggggcttgt tatatgcaag cggccggtcg tccatatcca gccggtgtga cagcgaaaga
     2461 catttaccgg gcctttacgg aaatgatgtt acaaacaacc gaccttgtta agcaagatcc
     2521 gacttacagt gaagaaggga ttatgacaac tgatgcccaa aaacgcattg atgacccgga
     2581 atcagttacc gctttagtct tcttcaaacg cgacaaagtt atttttgatc cgctttgggg
     2641 tatgctcggc gtttctactt atatcggtcc ggctattgat cgagtcgtta cacaaggtgc
     2701 cgactacaat gaagtattgg atgcccaaac aacgacttac caggcaaggc tcttggaact
     2761 ttatagttaa tgattgacta ggtgaaacca attgaggttt caccttttca tttggattag
     2821 cactgccaaa aatcttgata aaagtgctga ataatgttat aatagctaaa aaggtgacaa
     2881 catgaacttg gacaaaatgg ttaacagtaa atggtatacc ggttttgaat ggggatatcg
     2941 cttagttttt ttaaatttgc tgatgattac cattcccgcc ttgctgggag ctatcccttt
     3001 tctgttgtgg cgtcgttatc ctaatgccgg ttggctggtg attgtggcga ttgtcttaat
     3061 gctgtttggc tttattccct gttttattgc cgcttttcga gttattaaag cttatcggga
     3121 agaacaaacc ggcactttat ttggactttt tttcacatat ttatttaaca cttttaaaaa
     3181 catttacctt ttagaattaa tcatgttacc gctgttttgc ttatataccg ttgcggctct
     3241 gttttattgg gacatcattg gtaattatgc gcaattagat ctccccggtt gggtggcggt
     3301 tattagtttt gtagtgctgt tttt
//
LOCUS       DAOSGN010000046         3317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_26917_length_3317_cov_3.697425, whole genome shotgun sequence.
ACCESSION   DAOSGN010000046 DAOSGN010000000
VERSION     DAOSGN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26917_length_3317_cov_3.697425"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK087_01175"
     CDS             <1..485
                     /locus_tag="PK087_01175"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOH17912.1"
                     /translation="PGMNTSSIVGRTVDYEIINLGFSGSAHLEPEMATVIAQLKDIKL
                     LILEVEANAGDALVLKDRLENFVNIISQTQSHMKVMLISHYPYALAAYKAAIKARFVD
                     HYHFQKQLCHQKGWEFVDGEVLLRKLNFEESVDGVHLTDLGFYFLAVALKKRIKNLLG
                     "
     gene            536..2650
                     /locus_tag="PK087_01180"
     CDS             536..2650
                     /locus_tag="PK087_01180"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOH17913.1"
                     /translation="MKKYFFFILFFSFVFLSACNDSSLDEQPIDEQPTLESLAEEIKE
                     SIIFPKTTAENLHLPTTLTLTKATVSLKWQSSNEDAISLNGTIHPGLVAKQTNLKATM
                     TYQGEDLLIDFGFVTVAPWETSFVIAQIKQQIMLPKTTNTDVVLPTSVRVDQTNIPLT
                     WQSSLPAVLSNEGQVFALVNDTSVLLSVLAVVNNTTISFEYGSLTVLALSAEERIAKA
                     KAQISLPSETKTDLILPKNIDGVAINWFSSHPKIFTNKGEWTYQETDTTVALTAVFSY
                     GGLYEEETYLLDILTYTDEERLSKAYDQLSLPSTAEQNLELPIALAYGVTATWLSSHP
                     HIISNAGIVNLNEKENTVTLTATLKSGAKTMEKEFEITTKAISPSEVHFNGHMYTHRI
                     NDFILDGTNGLTINNKQVVLTDTSTAGTYESKIINTSPFTILVGSWSAISSTDATVEL
                     KVRVRVNGVWSSYLSYSAWGFGRQNAMLNQDGGVASMNADEIMIKNNAQANAFQFQVT
                     LRRNNVTTASPRLWLVGIALTIPNYSFPVDVSGLPNFVDYDVPKLYQHDVPTIGNSIC
                     SIASSTMLLLYHGHTFTNPLPHQETAALFRDYGNKIYGNWVFNTVGMSSYGETSYVKK
                     IYSWDELRYHLVHVGPVALSIKGDTGVYKTNGHLIVVRGYEIIGSETYVITNDPNIKG
                     VYYKFPLATFLNFTRNVIYVVE"
     gene            complement(2680..>3317)
                     /locus_tag="PK087_01185"
     CDS             complement(2680..>3317)
                     /locus_tag="PK087_01185"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOH17914.1"
                     /translation="SFPCPLPRFKGEFPYSQSLFQFSIHIEKGPNEADEFQDHRSFLV
                     SDSGDHREELINNLIDIIDLRNGGTVLVYNQNFEYSEIQKLAGMFPKYQEALNRICDH
                     IFDLKDLVKTSGTLFMGLGFSEKESKKVNYYHTQLGGKYSIKKLLPLFSNRSYADLDV
                     KNGNEAMAAYASFGKLSPEEIEEKRKQLIAYCRQDTWSMVEIINKLKQISD"
BASE COUNT         1010 a          697 c          619 g          991 t
ORIGIN      
        1 gccccggcat gaacacaagt tccattgtcg ggcgcactgt agattatgaa attatcaatc
       61 tcggtttttc cggaagcgct catcttgaac cagaaatggc gactgtcatt gcccaactga
      121 aggatattaa attgctcatt ttagaagttg aagctaatgc cggggatgct cttgttttaa
      181 aagaccgctt ggaaaacttt gtaaacatta tctcgcaaac acaaagccat atgaaagtaa
      241 tgcttattag ccattatccc tatgctttag ccgcttacaa agccgcaata aaagcccgct
      301 ttgttgatca ttaccatttt caaaaacaat tatgccatca aaaaggatgg gaatttgtgg
      361 acggagaagt tttattgaga aaactaaact ttgaggaaag cgttgatggc gtccatttaa
      421 cggatttagg tttttacttt ttagcagttg ccttaaaaaa acgaatcaaa aatttattgg
      481 gttaaattat ctctttctta aagcgaacta tgttataatt tatttaggtg atttcatgaa
      541 aaaatatttc ttttttatct tgttcttcag cttcgttttt ttaagtgctt gtaacgattc
      601 ctcccttgac gagcaaccaa ttgatgaaca gcccacttta gaaagcttag cggaagaaat
      661 aaaggaaagc attatctttc ccaaaacaac ggcggaaaat ttgcatctac caacaacttt
      721 gacgctaaca aaagcgaccg tttcactaaa gtggcaatcc agcaatgaag acgctatcag
      781 tctaaacgga accatccatc cgggtttggt ggcgaaacaa acaaacctta aggccacgat
      841 gacttatcaa ggtgaggatt tattgattga ttttggtttt gttactgttg cgccttggga
      901 aacatccttt gtgatagcac aaatcaaaca acaaataatg cttcctaaga ccaccaacac
      961 cgatgttgtt ttaccgactt cagtaagggt tgatcaaaca aatatcccct taacttggca
     1021 aagttctctg cccgccgttc ttagtaacga aggtcaagtt tttgctttag ttaacgatac
     1081 ttccgtttta ttatccgtct tagccgtcgt caataatacc accattagtt ttgaatatgg
     1141 ctctttgacc gttttagctt tgtcagccga ggagaggatt gcgaaagcga aagcgcaaat
     1201 ctctttacct tccgaaacaa aaacggattt aatccttccc aaaaatattg acggagttgc
     1261 tattaattgg ttttccagcc atccgaagat tttcacaaac aaaggtgaat ggacttacca
     1321 agaaacggat actaccgttg ccctcaccgc cgtctttagt tatggtggtc tctatgaaga
     1381 agaaacttat ttgcttgata ttcttactta cactgatgaa gagcgcttat caaaagctta
     1441 tgatcaacta agtttaccaa gcacggctga gcaaaacctc gaattgccaa tcgctttggc
     1501 ctatggagtg accgctactt ggttatcatc ccacccgcac atcatcagca atgccgggat
     1561 tgttaattta aacgaaaaag aaaacaccgt aaccttaacc gccaccttaa aatcaggagc
     1621 aaaaaccatg gaaaaagaat ttgaaattac tactaaagcc attagtccca gtgaagtcca
     1681 ttttaatggt catatgtata cccatcgcat caatgatttt attttagatg gaacaaacgg
     1741 actgactatc aacaataagc aagtcgtgct aaccgatacc agcacagctg gaacttatga
     1801 atctaaaatc atcaacacat cgccattcac aattttagtt ggttcatggt cagccatcag
     1861 tagtactgat gccactgttg aattaaaagt ccgtgtgcgt gttaatggcg tctggagttc
     1921 ttatttatcc tacagtgctt ggggatttgg tcgccaaaat gccatgctta atcaagatgg
     1981 cggcgtagct tccatgaatg ctgatgaaat tatgatcaaa aacaatgcgc aagctaatgc
     2041 ttttcaattc caagtcactt tacgaagaaa taatgtcaca accgcttccc cacgcctgtg
     2101 gttggttggg attgctttaa ctattcctaa ttacagcttc ccggttgatg tttccggatt
     2161 accaaacttt gttgattatg atgtcccgaa gctataccaa cacgatgtgc ccaccattgg
     2221 taattcgatt tgcagtattg catcctcgac catgttgctc ttataccatg gccatacttt
     2281 cacaaacccg ctacctcatc aagaaacagc ggcgctgttt cgtgattacg gcaacaaaat
     2341 ttatggcaac tgggtgttca atacggtggg catgagctct tatggtgaaa ccagctatgt
     2401 aaagaaaata tattcttggg acgagctacg atatcatctt gttcatgtcg ggccggtcgc
     2461 tcttagtatt aaaggtgata ccggtgtata taaaaccaac ggtcacttaa ttgttgtccg
     2521 tggatatgaa atcatcggtt cggaaaccta tgttattacc aatgacccca atatcaaagg
     2581 cgtgtactat aagttcccat tagcaacatt tttaaacttc acccgtaatg ttatttatgt
     2641 ggtggagtaa atagcaaaag gccctcctac gggccttttt taatcagata tttgttttaa
     2701 tttgttgatg atttccacca tcgaccaggt gtcctgacga caataagcaa tcagttgctt
     2761 acgtttttct tcaatttctt ccggcgacag tttaccaaag gaagcataag ccgccatggc
     2821 ctcgttaccg tttttcacat ccaaatcagc ataggagcgg ttggaaaaaa gcggcaacaa
     2881 ttttttaatg gaatactttc ctcctaattg agtatggtaa taattaactt ttttgctctc
     2941 tttttcagaa aaacccaaac ccataaacaa ggtgccggag gtcttaacta aatccttcaa
     3001 atcaaaaata tgatcacaaa tccggttcag tgcttcttga tacttgggaa acatccctgc
     3061 caatttttga atctccgaat attcaaagtt ttgattgtaa accaaaaccg tgcccccatt
     3121 ccttaggtca ataatatcaa tcaaattgtt aattaattct tcgcggtgat cccctgaatc
     3181 actaactaaa aaagagcggt gatcttgaaa ttcgtcggct tcattcggtc ctttttcaat
     3241 atgaatggaa aattgaaaca aggattgtga atagggaaat tcacctttaa aacggggcaa
     3301 cggacaagga aaacttt
//
LOCUS       DAOSGN010000047         3303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_27074_length_3303_cov_3.676416, whole genome shotgun sequence.
ACCESSION   DAOSGN010000047 DAOSGN010000000
VERSION     DAOSGN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27074_length_3303_cov_3.676416"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PK087_01190"
     CDS             <1..249
                     /locus_tag="PK087_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17915.1"
                     /translation="EVVSQYIKRVIVLKEGKIIYDGSKKQLFAKDDLMDKYNLNYPST
                     VKILRALEARFHCGLDVNQHSIEAAYAEICRCFGERHE"
     gene            242..1222
                     /locus_tag="PK087_01195"
     CDS             242..1222
                     /locus_tag="PK087_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOH17916.1"
                     /translation="MNSKVVFGQYYHVDSWMHRLDPRTKIVVIFLMIVSLFLLRQLVS
                     LLVAFIVTAVLVISSKVPFSKFLGSLKMITMLLFITVIFQVLLSKAGNAIPFALELNV
                     LNGSIILISFILFFLSRRFIRKGRFLLFVVLFLGSFVIQYYLIIGPKIINYKLVIYEA
                     GLLSSAKIITRIVSLIFISALLTLTTKPTDLNNGLESLLKPLKLLKVNVSIMTMMISI
                     SLRFIPTLFNEAEKILKAQASRGVDFKESRFKDKITQIVSLLVPMFVISYKRAGDLAD
                     AMEARGYIPEATRTSINQLTYKWFDILTLIVSVVMITGLIVVRVTGWYAL"
     gene            1212..1979
                     /gene="truA"
                     /locus_tag="PK087_01200"
     CDS             1212..1979
                     /gene="truA"
                     /locus_tag="PK087_01200"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOH17917.1"
                     /translation="MRYKCIVAYDGTLFHGFQTQGHLRTVQSEIEQVLLAINKEATMI
                     YPSGRTDAGVHAHGQVFHFDSAIDIPEQNMKNALNTRLPRDIYIKDVVIVDEDFHARF
                     SAIAKEYHYLIDFSERDPLLKNYRYYCPFHHVDIDSFIAAFKLFEGEHDFRSFTKNQQ
                     LSDTTRRIFQISAKQENALVTIKIVGNGFMRHMVRILVAMALEVGRNKITFERLKYIM
                     EQKNRFLSPKIVPSSGLYLFAVYYSQEELKQRLKQDN"
     gene            complement(2015..2914)
                     /locus_tag="PK087_01205"
     CDS             complement(2015..2914)
                     /locus_tag="PK087_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOH17918.1"
                     /translation="MELIILTNIFQSLIDSPFYRYAFIAGICVGVLAPLIGTVVVIRR
                     LSFIADTLSHFSLAGVAVGVFLAKIIPPSWFGLDPILMGVVFSVAGTFIIEKLRGFYK
                     NYKELSMPILLSFGVALSGLFIYLTPGISTNWTTGLLFGSIYSINARDIILILSLSVI
                     VITLSIIFYKKIVSLCFDEVYAQVSGINIRLFQLAITIILSLVISIFLDLVGVLLISS
                     LMIVPVAAAILVGDSFKNTIVSAIIFSEISVIGGFTLSYYLNLPTGSTIVLINILILL
                     IIMGISRIRKIKFKKDDDYLTPE"
     gene            complement(2905..>3303)
                     /locus_tag="PK087_01210"
     CDS             complement(2905..>3303)
                     /locus_tag="PK087_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH17919.1"
                     /translation="FSYEFPITVSEVLSSAYLHRKDAYYTSVINALDLNTFYRENVNT
                     LSGGQLQRVFIARALLNHPKLLILDEPTVGVDLENIKGVVKILTELKKQGVTIIISTH
                     EIEYVRDLTDYFLSMSDFGEYRFVEAGKWN"
BASE COUNT         1072 a          564 c          686 g          981 t
ORIGIN      
        1 gaagttgttt cgcaatacat taaacgtgta attgttttaa aagaaggaaa gattatttat
       61 gacggctcaa aaaaacaatt gtttgcgaag gacgatttaa tggataaata taatctcaat
      121 tatccgagta cggttaagat tttgcgagct ttggaagccc gcttccattg tgggctcgat
      181 gtcaatcaac actcaattga agctgcttat gcggaaatct gtcgttgttt tggtgaacgc
      241 catgaatagc aaagtcgttt ttggtcaata ttatcatgtc gattcttgga tgcatcgttt
      301 ggatccacgg acgaagattg tcgtaatttt tttaatgatt gtgtctttgt ttttgcttag
      361 acagctggtg tcgcttcttg ttgctttcat cgtgacggca gttcttgtta tcagcagcaa
      421 agtgccgttt tctaaattct taggcagttt aaaaatgata acgatgctgt tgtttatcac
      481 ggttattttt caggtgttac taagtaaagc cgggaatgcc atcccctttg ctttggaact
      541 caatgttcta aacggtagta ttattttaat cagttttatt ttgttttttc tttcgcgacg
      601 atttattcgc aaaggccggt ttttactgtt tgtggtattg tttttaggat catttgtgat
      661 tcagtattat ttaataatcg gaccgaaaat tatcaattat aagctcgtta tttatgaagc
      721 cggcttactt tcctccgcca agattattac aagaattgtt tctttgattt ttatttccgc
      781 attattaacc ttaacaacga aaccgacgga tttaaataat ggtttagaat cactattgaa
      841 accgctgaaa cttttaaaag ttaatgtcag cattatgacg atgatgattt caatttcctt
      901 aaggtttatt ccgacactat tcaacgaagc cgaaaaaatc ctgaaagccc aagcctcacg
      961 gggagtggac tttaaagaaa gtcgttttaa ggataaaatt acccaaattg tttctttgct
     1021 ggtgccgatg tttgttattt cttataaacg agccggcgat ttagccgatg cgatggaagc
     1081 aaggggctat attccggaag caacgcggac aagcattaac caattaactt ataaatggtt
     1141 tgatatctta acattaattg tgagtgttgt aatgataacc ggattgattg tggtaagggt
     1201 gacgggttgg tatgcgttat aaatgtatag ttgcttatga cggcacgctt tttcacgggt
     1261 ttcaaacgca gggtcattta cgaactgtcc aaagcgaaat tgaacaagtt ttactggcga
     1321 ttaacaaaga agcaacaatg atttatccgt cgggaagaac cgatgccggt gtgcatgccc
     1381 atgggcaagt ctttcatttt gattccgcaa ttgacatccc ggaacaaaac atgaaaaatg
     1441 cccttaatac acggttgcca agagatattt atattaaaga tgttgtgata gttgatgaag
     1501 attttcatgc ccgcttcagt gcgattgcga aagaatacca ttacttaatt gacttttccg
     1561 aacgagaccc ccttctaaaa aactatcgct attattgtcc gtttcaccat gttgatattg
     1621 acagttttat tgccgctttc aagttgtttg aaggtgagca tgattttcgt tcttttacaa
     1681 aaaaccagca attaagtgat actacgagga ggatttttca gattagcgcc aagcaggaaa
     1741 atgcccttgt gacgattaaa atcgtcggta atggttttat gcgccacatg gtgcgtattt
     1801 tagtggcaat ggctttagaa gtagggagga ataaaattac ctttgaaaga ctaaaataca
     1861 ttatggagca aaaaaaccgg tttttatcgc ctaaaattgt gccatcttca ggattgtatt
     1921 tatttgcggt ttattactca caagaagaat taaaacaaag gttaaagcaa gataattaaa
     1981 aaacgactga ttgaaaagta aatcagtcgt tttgttattc aggagttaaa taatcatcat
     2041 cttttttaaa tttaattttt cgaatgcgag aaatacccat aataatcaac agaatcaaga
     2101 tattgatgag gacaatggtg gaaccggtcg gcaagtttaa atagtaactt aaagtaaagc
     2161 cgccaataac cgagatttca gaaaagataa tggcggaaac aattgtattt ttgaaggaat
     2221 caccgacaag aatggcggcg gctacgggca caatcatcag tgaagatatt aacaaaacgc
     2281 caactaaatc caaaaaaatg gagataacta aggataaaat aatggtaatg gcaagttgga
     2341 agaggcgaat attgatgccg gaaacctgag catagacttc atcgaaacac aaagacacaa
     2401 ttttcttata aaaaataatg gataaagtaa tcacaatgac gcttaaactg agaattaaaa
     2461 taatgtcacg agcgttaatg ctgtaaatgg aaccgaacaa cagtcctgtt gtccagttgg
     2521 tggagatacc gggggttaaa taaatgaata atccactaag cgcaaccccg aaactaagga
     2581 gaatcggcat ggacaactct ttgtaattct tataaaaacc gcgtaatttt tcaataataa
     2641 aggtaccggc gactgagaaa accacgccca ttaaaatggg atcaagacca aaccagctgg
     2701 gggggattat ttttgctaag aaaacaccga cagcaacccc ggccaaggaa aagtgactga
     2761 gggtatcagc aatgaaggaa agccgccgaa tgacgacgac cgtcccgatt aaaggagcaa
     2821 gaacgccaac acaaataccg gcaataaatg cataacggta aaacggcgaa tcaattaaac
     2881 tttggaaaat gttggtaaga attattaatt ccattttccg gcctccacaa agcgatattc
     2941 accgaaatcg ctcattgaca gaaaataatc agttaaatcg cggacatatt caatttcgtg
     3001 agttgagata ataattgtta cgccttgttt ttttagttcg gtgaggattt tcacaacccc
     3061 tttaatattc tctaaatcaa cgccgaccgt cggctcatcc aaaataagta gtttggggtg
     3121 gtttaagaga gcccgggcga taaaaacgcg ttgcaattgg ccaccggata gggtgttgac
     3181 attttctcgg taaaaggtat ttaaatcaag agcattaata accgaggtat aataagcatc
     3241 tttacgatga agataagcgg aagagagaac ttcggaaacg gttatcggaa attcataact
     3301 gaa
//
LOCUS       DAOSGN010000048         3302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_27087_length_3302_cov_3.170003, whole genome shotgun sequence.
ACCESSION   DAOSGN010000048 DAOSGN010000000
VERSION     DAOSGN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27087_length_3302_cov_3.170003"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PK087_01215"
     CDS             complement(<1..882)
                     /locus_tag="PK087_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOH17920.1"
                     /translation="MLKWTKKWILVMIIVTLGLGLSACGDDYQVYTLNLSDSFNTTNK
                     VIVISETKTEAELNKLYNELDQILINLDLMFNVQDRGDGVVTELMRLNAQAGIAPMLV
                     SDELLMVVKKTLEISNYSQVDGTYLFDPTIAPVWDNWHFIDKLYDSFLDNRAQAPDEA
                     TITALLPLVNRENIEINEEQKTIFLKVAGMKLDLGAIVKGYAADKLKKYLVDLGYKKA
                     VIDVGRNILLLGSGINPDGQDMDWKVDVQTPFVGMFDDNAVRTYGTMRLSDVTVVTSG
                     IYEKYIKDDEGRMYHHIL"
     gene            complement(968..1597)
                     /locus_tag="PK087_01220"
     CDS             complement(968..1597)
                     /locus_tag="PK087_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17921.1"
                     /translation="MTIINNEQLNHLLAKHDEKTQLFVSELNTLNNEYFPLFTNLKKE
                     RGNRLYGMGVVICLLAFLSFMVIFGAIEPLKLIYLLYIITGGLLVYSLVLVFKTQKEL
                     KTLKTDWDQKYQVIYQIKLAAYEILAEIKEQILYVIATNEQKTLDEVTKMMETHIGST
                     LAPDEMIAYYEAWGKAKTGQTTINLDELREMRRKKAEIMTKKKTSTVNP"
     gene            complement(1700..3100)
                     /locus_tag="PK087_01225"
     CDS             complement(1700..3100)
                     /locus_tag="PK087_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17922.1"
                     /translation="MKKFSLFLGMLLMVLSIGLYGCTIGSANTAYAADAYVVLDINPS
                     VEILADGKGLVIQVNPLNEDAETLLVDTDFVGKTVDETVEAIVALALELGYLDFDVEN
                     AIVITAACSNDENTKKLEAKIHNRIRGYADKKGMSMEVIHARQEADEEMLALAESLEI
                     SIGKLKLIQLAMQFDETLTLELGATMAVKDLNAIVKANRQEMKEFVGEQLRNRFMEFH
                     QEARGTFGVKRVQFIYDAMVLAEADFFAPVLTESTATAADLIALYEEYLNALKAIETP
                     KPTEEVIIEAEATLEIALDEDLANLLKQNQTLMQEMLQIKAQLGKAKKGSDQSAQLRA
                     QLQAKWKEIQANNEQIQACKEQYRVEFKGNHKGYEFGIHAGKGEFVVAARFDWINAFR
                     TIRIEYEAKFAEIKVSLTDLEALFEEIVEPQLATVRAECQADFGALKAEVKGQADAIR
                     EQFRENKGNKHGMWGK"
BASE COUNT          959 a          714 c          534 g         1095 t
ORIGIN      
        1 taaaatgtga tgatacatcc ggccttcatc atctttaata tatttttcat aaattcctga
       61 agtgacgacg gtcacatcgc ttaaacgcat cgttccgtaa gtgcgcaccg cattgtcgtc
      121 aaacatcccc acaaacggtg tttggacatc aactttccag tccatatctt ggccatcggg
      181 attaatgccg ctccctaaca gtaagatatt acgtcccaca tcaatcactg cttttttata
      241 gcctaagtca acaaggtatt tttttaattt gtcggccgca tagcctttca caatcgctcc
      301 taaatcgagt ttcatccccg caacttttaa gaaaatagtt ttttgttctt cattaatctc
      361 gatattttcc cgattaacca agggtaaaag agctgtaatc gtcgcttcat caggcgcttg
      421 ggcccgattg tcaagaaagg aatcatagag tttgtcaata aaatgccaat tatcccaaac
      481 tggagcaatt gtcgggtcga ataaataggt accgtcaact tgcgaataat tactaatttc
      541 taaagtcttt ttcacaacca tcaacaattc atcactgaca agcatgggag caatccccgc
      601 ttgggcgttt aaacgcatta attccgtcac aacgccatcc ccacggtcct gaacattaaa
      661 cattaaatcc agattgatta agatttgatc cagttcatta tataatttat ttaactcagc
      721 ttccgttttc gtttcactaa tcacaatcac tttattggtg gtgttgaaag aatcacttaa
      781 gtttaaagta taaacttgat aatcgtcccc acaagctgaa agacccaagc ctaaagtcac
      841 aattatcatc actaaaatcc attttttggt ccactttaac ataaataaac ctcaatttgc
      901 ttcctttaca tattatactc ttctctacaa aaaaatgcat taaaagtaat aatcaaaaaa
      961 gtcaacccta ggggttgacg gtcgaggtct tctttttcgt catgatttcg gcttttttac
     1021 gcctcatttc ccgaagttca tcaagattga tggttgtttg accggttttc gcttttcccc
     1081 aggcttcata ataagcaatc atttcatccg gtgctaaagt cgaaccaata tgtgtttcca
     1141 tcatttttgt aacttcatca agcgtttttt gttcattagt agcaatcaca tataatattt
     1201 gctctttaat ttctgccaag atttcataag ccgctaattt aatttggtaa ataacttgat
     1261 acttttggtc ccaatccgtt ttaagggttt ttagttcttt ttgggtttta aaaaccaaaa
     1321 ctaagctgta aactaaaaga ccgccggtaa taatatagag taaataaatt aacttaagcg
     1381 gttcaatcgc cccgaagata accataaaag ataaaaacgc aagcaaacaa ataacgactc
     1441 ccattccgta taagcggttc ccccgttctt tttttaggtt tgtgaagagg gggaaatatt
     1501 cattattgag ggtatttaat tcgctgacaa acaattgcgt tttttcatca tgcttcgcta
     1561 ataaatgatt taattgttcg ttattaatga tggtcatatt aatttcctca ctagttttat
     1621 tataacacaa tcaccttaaa accaaaagaa aaagttaaga gcgggggact cttaactttt
     1681 ttgtatgttt agggctggtt tattttcccc acataccatg tttattacct ttattctccc
     1741 ggaattgttc gcggatggca tcggcttgac ctttaacttc agctttcaaa gctccaaaat
     1801 cagcctgaca ttcagcgcga actgttgcca actgtggttc aacaatctcc tcgaataaag
     1861 cttctaaatc agtaaggcta actttgattt ctgcaaactt agcttcatat tcaattctaa
     1921 ttgtgcggaa ggcattaatc caatcaaagc gggctgctac gacaaattcg ccctttccgg
     1981 catgaatacc gaattcataa cctttatgat tgcctttaaa ttcaactcga tattgttctt
     2041 tacaagcttg gatttgctcg ttgttagctt ggatttcttt ccattttgct tgtaattggg
     2101 cccgtaattg ggcggattgg tcactacctt ttttagcttt acctaattgg gctttgattt
     2161 gtaacatttc ttgcatcaat gtttggtttt gttttaacaa attagctaaa tcctcatcca
     2221 aagcgatttc caaagttgct tcggcttcaa taataacttc ttccgttggt tttggtgttt
     2281 ctatagcttt aagagcattg agatattctt cataaagagc gattaaatca gcagcggtgg
     2341 cggtcgattc cgttaatacc ggagcaaaga aatcggcttc agcaagtacc atggcatcat
     2401 agataaattg cacgcgctta accccaaaag ttcctcgggc ttcttgatgg aattccataa
     2461 agcggttccg caattgttcg ccaacaaatt ctttcatttc ctgacgattg gctttcacaa
     2521 tcgcattaag atctttaact gccatggttg cgccaagttc aagcgttaag gtttcatcaa
     2581 attgcatggc tagttggatt aattttagtt ttccaatact gatttccaac gattccgcta
     2641 aagcaagcat ttcctcatca gcttcttgac gggcatgaat gacttccatc gacatcccct
     2701 tcttatcggc ataaccccga attcgattat gaatcttagc ttccagtttt tttgtatttt
     2761 catcatttga acaagcagcg gtgatcacga tggcattttc aacatcaaag tctaaataac
     2821 ctaattctaa agctaaggcc acaatcgctt ccaccgtctc atcaacggtt ttgcctacaa
     2881 aatcagtatc caccaacaat gtttccgcat cttcatttag ggggttaacc tgaataacca
     2941 aacctttacc atccgcaaga atttctacgg atgggttgat atctaaaaca acataggcat
     3001 cggcggcata agcggtatta gccgatccta tcgtacaacc atataaacct attgataaaa
     3061 ccatcaagag cattcctaaa aataaactaa attttttcat ctttcttctc cttttctttt
     3121 ggcactaata taacaattag ctatgttttt ttgctagtaa aatttttatt ttcctaaata
     3181 ttgttgatat ttttctaaaa agtccgtaac cacctggctt tgtttgaaag catccgtgac
     3241 caacggcgaa tccatcaaag cttcaaactg agtcatcaac tttgaaataa cgcggtcgga
     3301 gc
//
LOCUS       DAOSGN010000049         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_27192_length_3293_cov_2.865658, whole genome shotgun sequence.
ACCESSION   DAOSGN010000049 DAOSGN010000000
VERSION     DAOSGN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27192_length_3293_cov_2.865658"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PK087_01230"
     CDS             <1..811
                     /locus_tag="PK087_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17923.1"
                     /translation="KILNIIKSNATEPFKILWSVFEELSLLTSNPDMSFSKASKYFYT
                     TFTSYEKYFKKLALIASFHKGVTKKVPLFTIKTRLSNLYVKETEYLNEALLDLYMECR
                     DNKHDEYTYACMRYIFAPFWSSYLINSSDITQRKPGFLETLKNYAISQLTNWEKVFDK
                     NVDDFIFENNLAIKYESNIVESIFSISCPTLNECLKEVNENTSRENVEMIKDIITQLK
                     ETFAYEEIIYKEWYDINALSYEFLNLFYHSCCENKRQMRIIIIFALSKSLC"
     gene            833..1426
                     /gene="def"
                     /locus_tag="PK087_01235"
     CDS             833..1426
                     /gene="def"
                     /locus_tag="PK087_01235"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOH17924.1"
                     /translation="MLTYQEIIKEEDPRLREKALAVNLPLAAEDIALLETLNAYLVAG
                     YDPEYAEQNDIRPGVGLAAPQLGIRKQMFVVVAFDENGELFHFGVINPKIVSHSEEKV
                     YLPSGEGCLSVDREVQGLVHRSKRISLSAHYYNFTTKELTKQTLKLKDYIAIVCQHEL
                     DHLHGILFVDHIDPINPFFIPDNSTAIVFPASETKEN"
     gene            complement(1447..2136)
                     /locus_tag="PK087_01240"
     CDS             complement(1447..2136)
                     /locus_tag="PK087_01240"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HOH17925.1"
                     /translation="MSNLAIIIIIGETILMLFYLIFYYRWGSKTVLGLKAATSLGFVV
                     LGLLNLKQANELRAGTTVFIGLVCGFLGDIILGFRHLYRSHKRELFYGGLLLFFVGHV
                     VYILAFLSLRSGQGFLIVLVALVLLAVVYLLSRHYHIDYGDAKNAVFAYMAVSSLLLS
                     LVFINVISAYSKLKLLIFIGALSFVISDFLLSFIYFRKMSRPKIRLFKAVNIVSYYLG
                     QTLFAVSICLM"
     gene            complement(2153..2932)
                     /locus_tag="PK087_01245"
     CDS             complement(2153..2932)
                     /locus_tag="PK087_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOH17926.1"
                     /translation="MNNKKLILLDIDGTLFDNQRQMVSPKSIAAIKQLHNHHIVAIAT
                     GRAPFMIDSIKAIKPLIDYYIFINGQYICAHKKTIFQEPLDRNVLKELIKRLQSLNLA
                     YGFESSHQEAVSRIDELVLYSFQNLGLPVPPVDPQFYLHHDVFQAWAFSNSGAIDMLK
                     AEFSELRFIQWMDVGYDILPGHASKGLGMKRLAEYLQMDLKDVIAIGDGDNDFEMVRD
                     AGIGIAMGNAAEKVKRVAKIVTSDVGDDGVYQALMSIGLIK"
     gene            3089..>3293
                     /locus_tag="PK087_01250"
     CDS             3089..>3293
                     /locus_tag="PK087_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOH17927.1"
                     /translation="MERFNDNNIKIFALGGLDEIGKNMYVVECDQELIIIDAGIMFPG
                     DNYGIDYIIPDYTYLMDNVDKIIG"
BASE COUNT         1128 a          663 c          558 g          944 t
ORIGIN      
        1 gaaaattctt aacatcatta aatctaatgc cacggaaccg tttaaaatct tgtggtcagt
       61 atttgaagaa ttgtctctgt tgacgtctaa tccagacatg agcttttcaa aagcttccaa
      121 gtatttctat acaactttta cgagctatga aaaatacttc aagaaacttg ctttgatcgc
      181 ttcattccat aaaggtgtaa ccaagaaggt tcccttgttt acaattaaaa caagactttc
      241 aaatctttat gtaaaagaaa ctgaatatct taatgaagcc ttgttggatc tttatatgga
      301 atgccgtgat aacaagcatg atgagtatac atatgcatgt atgagatata tcttcgctcc
      361 attctggtct agttatctta tcaactcatc agacatcact cagaggaaac caggcttcct
      421 tgaaactctt aaaaattatg ccatcagtca gttaactaat tgggaaaaag tctttgataa
      481 aaatgttgac gatttcatct ttgaaaacaa tctggccatt aaatatgaaa gtaacattgt
      541 tgaaagtatt tttagcattt catgcccgac tctgaatgaa tgtctcaaag aggtcaatga
      601 aaacacctcc agagagaatg ttgaaatgat taaggatatt attacccaac tcaaagaaac
      661 atttgcctat gaggagatta tatacaagga atggtatgat attaatgctc tttcatatga
      721 attcctcaat ttattttatc attcttgttg tgaaaacaaa aggcaaatgc ggataatcat
      781 aatatttgca ttatcaaaat ccctgtgtta aaattaaaaa cgaggtaaat atatgttaac
      841 atatcaggaa attattaaag aagaggatcc ccgattacgc gaaaaggccc tagcggttaa
      901 cttaccatta gcagcagaag acattgcttt attggaaact ttaaacgctt atcttgtcgc
      961 cggttatgat cctgaatatg ccgaacaaaa cgatattcgt cccggtgttg gcttggcagc
     1021 accacaactg ggaattcgca aacaaatgtt tgtggtcgtt gccttcgatg aaaatggaga
     1081 attgtttcac tttggcgtca ttaaccccaa aatcgtctcc cattccgaag aaaaagttta
     1141 tctgccaagt ggtgaagggt gtttatccgt cgaccgcgaa gtccaaggtt tagtccaccg
     1201 ttcaaaacgc atttcccttt ctgctcatta ttataatttc acaactaaag aactaaccaa
     1261 acaaacccta aaattaaaag attatatcgc tattgtttgt cagcatgaac tagatcacct
     1321 tcatgggatt ttatttgttg accacatcga tcccattaat cccttcttca ttcccgacaa
     1381 cagcaccgcg attgtttttc cggcatcgga aacaaaagaa aattaaaagc tcgccccgcg
     1441 agctttttac attaagcaaa tgctgacagc aaacagcgtt tggcctaaat aataagacac
     1501 aatgttaaca gctttaaaaa gacggatttt gggacgactc atttttctaa aataaataaa
     1561 actgaggaga aaatccgaaa tgacaaaaga taatgcgcca atgaaaataa gtagtttaag
     1621 ctttgaataa gcactaatca catttataaa aactaacgac aacaacagtg aactaaccgc
     1681 catataagca aaaacagcgt ttttcgcatc accgtaatca atatgataat gacgagaaag
     1741 caaataaacc accgccagca aaacaagcgc caccaaaaca attaaaaaac cttgaccact
     1801 tcgtaacgat aaaaaagcga ggatataaac aacatggcca acaaaaaaca acaataagcc
     1861 cccataaaag agttctcttt tgtgactgcg gtaaagatgc ctaaaaccaa ggatgatgtc
     1921 ccccaaaaag ccgcaaacaa gtccgataaa aaccgtcgtt cccgctctta gctcattggc
     1981 ttgttttaag tttagtaatc ccaacacaac aaagcctaaa ctagtcgccg cttttagacc
     2041 caaaacagtt ttactacccc aacgataata aaagattaag taaaaaagca tcaagatcgt
     2101 ttcaccgata ataatgataa ttgccagatt ggacactttt atttctcctt tgctatttga
     2161 ttaaaccaat actcataagg gcttggtaaa ccccatcatc accgacatca ctcgtcacga
     2221 ttttcgcaac ccttttcact ttttcggccg catttcccat ggcaatgccg atgccggcat
     2281 cacgaaccat ttcaaaatca ttatcgccat cgccgatggc gataacatct tttaagtcca
     2341 tttgtaaata ctccgccaag cgcttcattc ctaaaccttt tgaagcatgt ccgggtaaaa
     2401 tatcatagcc gacatccatc cattgaataa aacgcagttc cgaaaactcc gctttaagca
     2461 tgtcgatggc accggaattg gaaaaagccc aagcttgaaa aacatcgtga tgcaaataaa
     2521 actgggggtc aaccggcgga accggaagtc ctaagttttg aaaactatac aaaacgagtt
     2581 cgtcaatccg tgataccgct tcttgatggc tgctttcaaa accataagct aaatttaaac
     2641 tctgcagacg cttaattagt tcctttaaaa cgtttcggtc taaaggttct tggaaaatgg
     2701 tttttttatg agcacatata tattggccat taatgaaaat ataataatca attaagggtt
     2761 tgatagcctt aatagaatca atcatgaacg gtgcacgacc cgtagcaatg gcaacaatat
     2821 ggtgattatg tagttgttta atggcagcaa tgcttttagg cgacaccatt tgtcgttgat
     2881 tatcaaaaag cgttccgtca atatccaata aaattaattt cttgttattc atataatccc
     2941 actttggtct attctagcat gtttggaaag ataaaacaag ataataattt tgcttttatc
     3001 ttcccttttg gaacgatatt gattataata agcctaaaga cgaaaggaga ctatcgtggg
     3061 tactatcaat aacaaacaac ttcccatcat tgaacggttc aatgataata atataaaaat
     3121 ctttgccttg ggtggacttg atgaaatcgg caagaacatg tatgtggtgg agtgcgatca
     3181 ggaattgatc atcattgatg cgggaatcat gttcccgggc gacaattatg gcatcgatta
     3241 catcattccc gattatactt acctcatgga taatgttgat aaaatcatcg gtt
//
LOCUS       DAOSGN010000050         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_27439_length_3273_cov_3.114978, whole genome shotgun sequence.
ACCESSION   DAOSGN010000050 DAOSGN010000000
VERSION     DAOSGN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27439_length_3273_cov_3.114978"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /locus_tag="PK087_01255"
     CDS             <1..1967
                     /locus_tag="PK087_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH17928.1"
                     /translation="KTSYSDLDVYTLGVDEKINFGIIHAGSHSLLFYPFAQISQVWEK
                     CSDALKSDENLKYVLLAPDDILTKELVHFITKDYPQVVFLSDADTIHRYQLLFPHLSY
                     QLVKKDDILALSPNHQLKFIFTPFIAQFNSFIVFDLKANILISNLLFSNPNNNLNNAQ
                     KALLAFHEQRIPSSDFLRPLMKNVQKLKPQLIIPYYGEPLSNQQIPATITLLANFVFY
                     NSTYLVKRVSGKRRIFNYLNLGNQILIQLKNLYGEAEVSAVFQGTDIEIDPTSMEITK
                     APTSNEQLWHNLFSIILDKKGPAWLAVLEKTVNKLNSLYNIKKPNVYRSLLVSSKFKA
                     LELQSQFSSLQESYNEVTDQLSRCPLTKAFNEMFLRNLLRKKINDIIETNSEDDWVLL
                     FIDIDNIRKLNLIHSKEIGDETIRNLYYLLNQVKEDQQDIFKRNGPGFVLLCPQYDYA
                     KSLKLAEKIRNAVETSDTFIERITVSVAIVRLHEFPPDQRKTLDMADLWFERGETRIK
                     LAPSLGFNHIIDERIKGSVLSIGKILIVDTDEIIHNILELALNSINYEVVIAKNGMHA
                     LEIAQTQIIDLIICEKNIPKLDGFQIKSRLNLSLETQTIPFILTTFNKNKEIVVSANQ
                     LGIDIILTKPLIVEEVLGLVSRLTAQKVRL"
     gene            1964..>3273
                     /locus_tag="PK087_01260"
     CDS             1964..>3273
                     /locus_tag="PK087_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17929.1"
                     /translation="MISLQLILAILWLMLLLALAIILLIFANKIKLRFDLKQSSKIKG
                     YLIDKYVNHKPLKKRFPKRKLTDEFINICEQIQLEPVLREEIIADFHTLNIVNFFISQ
                     LNSWHVFKRKYAAHYLGFFASKEALSALANRLNKEKNNSVKLYLLNGLRHHLDDYLEE
                     ALNSLLGANQFYRTRAIGIIKQHHNQFSSTLQAALNRPEFEIKELVVAYSDMIKSNTL
                     YDFLKKEQQKVEEFLFLDVPVPVDYASQHFDVRQYYLTILKNLVHIYHEDIIKDYYLS
                     HPDAGVRRIVVDAKSKQVNFDGILAILNGCDGSEVDNYRIEVITKIAETNRDLYMSLV
                     GIFPNADNIYQQKVIASVLSFRMDYLILKLTSAQRNQIIKALKLIIGFGLNADLIDFL
                     NRNKDPNIEKELINILLAVLPRDDKLQQELNLYLREELLWKLGIT"
BASE COUNT         1088 a          638 c          519 g         1028 t
ORIGIN      
        1 ccaaaactag ttattctgac ttggatgttt acactcttgg cgttgatgaa aaaatcaatt
       61 ttgggattat tcatgcgggc agccattcgc tgctttttta tccgtttgca caaatcagcc
      121 aagtatggga aaaatgttcc gatgctctaa aaagcgatga aaacttaaaa tatgttttat
      181 tagctcccga tgatatctta actaaagaat tggtgcattt tattaccaag gattatccgc
      241 aggttgtttt tttaagcgat gccgacacca tccaccgtta tcaactgtta tttcctcatc
      301 tatcgtatca gcttgtgaaa aaagatgata ttctagctct cagtcctaac catcaactta
      361 aatttatctt taccccgttt atcgctcaat ttaattcatt cattgttttt gatttaaaag
      421 ccaatatctt aatcagcaac ctgctttttt ccaatccgaa caataaccta aacaatgccc
      481 aaaaagcctt gctggctttc catgaacaac gcattcccag cagtgatttt ttgcgtccat
      541 taatgaaaaa tgtccaaaaa ttaaaacccc agctaattat tccctattat ggtgaacccc
      601 tatccaacca acaaattccc gctacgatta ctctcttagc caatttcgtt ttttacaact
      661 ccacttatct tgtaaaaagg gtgagtggca aacgccggat ttttaactac cttaatcttg
      721 gcaatcaaat cttgattcaa ttaaaaaact tgtacggtga agcggaagtt agtgctgttt
      781 ttcaaggaac ggacattgaa attgatccaa cctcaatgga aattactaaa gccccaacca
      841 gcaatgagca actttggcac aatttattca gtatcattct tgataaaaaa ggtcccgctt
      901 ggttagcggt tttagaaaaa accgtcaaca aactgaatag tttgtacaat attaaaaaac
      961 ctaatgtcta tcgatctttg cttgtttctt ccaaatttaa agccctcgaa ctgcaaagtc
     1021 agttttcctc attacaggaa agttataatg aagttacgga ccaattaagt cgttgtccgc
     1081 tgactaaagc cttcaatgaa atgttcttaa gaaatttgtt acgcaaaaaa atcaatgaca
     1141 tcatcgaaac taattctgaa gacgattggg tactgctgtt tattgatatt gataatatcc
     1201 gcaaattaaa tctcatccac tccaaagaaa tcggtgatga aacaattcgc aatttatatt
     1261 atttattaaa ccaagttaaa gaagaccagc aagatatatt taagcgcaat ggtcccggct
     1321 ttgttttgct ctgcccgcaa tatgattatg cgaaaagttt aaagcttgct gaaaaaatcc
     1381 gcaatgcagt ggaaaccagc gatactttca tcgaaagaat aaccgtcagt gtcgctattg
     1441 ttcgcttaca cgaatttcct cccgaccaaa gaaaaaccct tgacatggcc gatttatggt
     1501 ttgaacgggg cgaaacaaga attaaactag ccccatcatt agggtttaac cacatcattg
     1561 atgaacgcat taaaggctct gttttatcca ttggtaaaat cctcattgtt gataccgacg
     1621 aaatcattca taatatcttg gaattagcct taaatagtat taattatgaa gttgttattg
     1681 cgaaaaacgg catgcacgct ttagaaatag cccaaacaca aatcatcgat ttaattattt
     1741 gcgaaaaaaa cattcctaaa ttagatggtt ttcaaatcaa aagtcggctt aacctcagtc
     1801 tagaaaccca aacaattcct ttcattttga caacttttaa taaaaacaaa gaaattgtcg
     1861 tttccgcaaa ccaattaggc atcgatatta ttcttaccaa gcccctaatt gtggaagagg
     1921 ttttaggatt ggtgagtcgt ttaacagctc aaaaggtgcg cttatgattt ctttacagtt
     1981 aattttagcc atcctttggc tcatgttatt gttggcccta gccattattt tactcatttt
     2041 tgctaacaaa ataaaactgc gctttgattt aaaacaatct agtaaaatca aaggctattt
     2101 gattgacaaa tatgtcaacc ataaacccct taaaaaacgt tttcccaaac gaaaactaac
     2161 ggatgaattt attaatatct gtgagcagat acagctagaa cccgtattaa gagaagaaat
     2221 cattgcggat tttcatacac ttaatatcgt caatttcttt atttcacaac ttaattcttg
     2281 gcatgttttc aaacgaaaat atgcggctca ttacttaggc ttctttgctt ccaaagaagc
     2341 actttcggcc ttagccaacc gtctaaataa agaaaaaaac aatagcgtaa aactatactt
     2401 attaaatggg ctgagacatc atttagatga ttatctcgaa gaagctctta acagtttgct
     2461 tggtgccaat cagttctacc gcacacgggc tatcggtatc atcaagcaac atcacaatca
     2521 atttagctcc accctacaag cggctttaaa ccgtcctgaa tttgaaatca aagaacttgt
     2581 ggttgcttat tctgatatga tcaaaagtaa taccctctat gattttttaa aaaaagaaca
     2641 acaaaaagta gaagagtttt tgtttttaga tgtacccgtg cccgttgatt atgcctccca
     2701 acattttgat gtccgccaat attatttaac gattttaaaa aatttagtcc acatttatca
     2761 cgaagacatc atcaaagatt attacttaag ccatcccgat gctggtgtgc gccggattgt
     2821 tgttgatgct aaaagcaaac aagttaactt tgatggcatc ttagccatct taaatggttg
     2881 tgatggcagt gaagttgata attatcgcat cgaggtgatt accaaaatag cggaaaccaa
     2941 ccgcgacctt tacatgagcc ttgttggcat tttcccaaat gccgataata tctatcaaca
     3001 aaaagtcatt gcttctgtat tatcatttag aatggattac ttaattttaa aattaacttc
     3061 tgcgcaacga aatcaaatca ttaaagcttt aaagctaatt attggttttg gtttaaatgc
     3121 cgatttgatt gactttttaa atcgtaataa ggatccaaat atcgaaaaag aattaataaa
     3181 tattttgctt gcggtattac caagagatga taaattacaa caagaactca acctgtattt
     3241 aagggaagaa cttttatgga aactaggcat aac
//
LOCUS       DAOSGN010000051         3269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_27496_length_3269_cov_3.199440, whole genome shotgun sequence.
ACCESSION   DAOSGN010000051 DAOSGN010000000
VERSION     DAOSGN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27496_length_3269_cov_3.199440"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="PK087_01265"
     CDS             <1..1529
                     /locus_tag="PK087_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17930.1"
                     /translation="SPNDLFIYTEYDPAGVGTDIIYIVDAVSNLLFVFDGNMRYQYKV
                     ANYEILPENFTDDELIKMKTAFVDNNKNVTSISFTDYLAKKGMTLVDFRAIAQIPYAM
                     RTPAQKFYLSPNALSGVYRNVRPAKNPDGTIIRGVFQDLLYLSDKSNNQIVIVDAKTY
                     QVVQVVSSPTDISFSGKVFLPVKLVTDVAGRIFVISEGVYEGIMQMSYEGNFSSFVGV
                     NYVSLTFWELFWRRFMTDEQLAQTETILNTIFTSLTIDEEGFIYATSRAVGTNAGAMI
                     KRINPSGKDVLTRNGYSVPMGDLTYIRTGTNVALRGPSRFSAVTVNDYGVYTIADEKS
                     GRLFSYDDEGNLLYISGGRGNELTNLNTPIAIRYQGENILALDKEKQSVIRFEPTDIA
                     VSINKAVRYHYIGNLVASSEEWKNVVHQNPNYEYAYVGIGKSLLNEGRYLEAMSYFQT
                     GYSVDYYSKAYKLYRDEQVNRYFTPVMSVILVGVAAWMGYKLYRKIKFGKDEDEGGEE
                     "
     gene            1526..2224
                     /locus_tag="PK087_01270"
     CDS             1526..2224
                     /locus_tag="PK087_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOH17931.1"
                     /translation="MMNNNETLKNRMKINWPKIKQFFAFVYEEFIRFPTYILIHPLKG
                     FDAFKREKKAKMSVALIFVVLLIILQILEFQYTGFVVNQLELKDLNSFREIASVIVPI
                     VAFVVGNWSITTLFDGKGKMKEIFMMICYSLFPLIVSKILGMIFSNMIVVEEAGFYHL
                     IIGLGTFLMGYMIFFGLISIHEYGLFKCLLSILGTLIAVMVLFFVVMLCFDLFQKMVG
                     FIYTLYREIALRYL"
     gene            2237..>3269
                     /locus_tag="PK087_01275"
     CDS             2237..>3269
                     /locus_tag="PK087_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17932.1"
                     /translation="MKVFKLKNILALSLIVIITVLLLIFFIGGLKAKALALNNLEFDL
                     TGFVDYSPYDRQGNYVVEYDSAAEANAAVAGLPETSYTKVINEASDIYTVTFKLNKER
                     IVAQNNLYTMFLNEDTTIVSVAVNSECTPTAEKTAEGFTNYKKATCKTVFNSAFDSGN
                     SSDMKSNFILYYIGSNDKLSGTGFNTLANSVLYNDLLYGTIKRHYQIKYDVENGIEIL
                     YEIGDFTVINSFFPEDFKRTDMEDYFRGNLLFLINAGQVNQNKYEYADLAYTWSQECA
                     AYLEEKGLATVTPVFEGETFVDEDGNPIPSRYNLTNIIAKDEEGRPLNKLKMQAGLDY
                     NATNYGEEGA"
BASE COUNT          938 a          605 c          689 g         1037 t
ORIGIN      
        1 ccagtcctaa tgatttattc atctataccg aatatgatcc tgcgggcgtg gggaccgata
       61 ttatctatat cgtcgatgcg gtttccaact tgttgtttgt ttttgacggc aatatgcgtt
      121 atcaatacaa agtcgctaat tatgaaattt taccggaaaa ctttaccgat gatgaactca
      181 taaaaatgaa aacggctttt gttgacaaca acaaaaatgt aacatcgatt tcctttaccg
      241 attatttggc caaaaaaggc atgacattgg tggattttcg ggctattgcg caaatcccct
      301 atgctatgcg aaccccagcg caaaagtttt atctaagccc taacgcctta agcggtgttt
      361 accgcaatgt tcggccggcc aaaaatcccg atggaacgat tattcgcggt gttttccaag
      421 atttacttta tttatccgat aaaagcaaca atcaaattgt gattgtcgat gccaagactt
      481 accaagttgt tcaggttgtc tccagtccca ccgatatcag tttttcggga aaagtttttt
      541 taccggttaa attggtgacc gatgttgccg ggcgcatctt tgttatctcg gaaggtgttt
      601 atgaaggcat tatgcagatg tcctatgaag ggaacttcag cagctttgtc ggtgttaact
      661 atgtgagttt aactttttgg gaattgtttt ggcggcgctt tatgaccgat gaacaattag
      721 cccaaacgga aacgatttta aatacgattt ttacttcctt aacaattgat gaggaaggtt
      781 tcatttatgc gacttcgcgg gctgttggta cgaatgctgg ggccatgatt aaacgcatta
      841 atccctccgg aaaagatgtc ctaacccgca acggttattc ggtcccgatg ggagatttga
      901 cctatattcg taccggtacg aatgttgccc tccgcgggcc atcgcgcttt tcggcagtaa
      961 ccgtcaatga ttacggggtt tataccatcg ctgatgaaaa atcggggcgt ttattctcct
     1021 atgatgatga agggaacctc ttatatattt ccgggggtcg cgggaatgaa ctaacgaatt
     1081 taaatacgcc gatagcaatt cgttatcaag gtgaaaatat tttggcactt gataaagaaa
     1141 aacaatccgt gattcgtttt gaaccaacgg acattgccgt ttccattaat aaagcggttc
     1201 gttatcacta tatcggtaat ctagtggcct cttcggaaga atggaagaat gtcgttcacc
     1261 aaaatcccaa ttatgaatat gcttatgtcg gtatcggcaa atcgctgtta aacgaaggtc
     1321 gttacttgga agccatgagt tatttccaaa ccggctacag tgtcgattac tattccaaag
     1381 cctataaact ataccgtgat gaacaagtta atcgctattt tactccagtg atgtcagtca
     1441 ttttagtggg agttgctgct tggatgggct ataaactata ccgaaaaatt aaatttggaa
     1501 aagatgaaga tgaaggcggt gaggaatgat gaataacaat gaaaccttaa aaaaccgcat
     1561 gaaaataaac tggccaaaaa taaaacaatt tttcgccttt gtttatgaag aattcattcg
     1621 ctttccgacc tatattttaa tccatccttt gaaaggcttt gatgctttta agcgggaaaa
     1681 gaaagcgaaa atgtcggtgg cccttatttt tgttgttttg ttaattatcc ttcaaatctt
     1741 ggagtttcag tacaccggct ttgttgtcaa tcaactggaa ttaaaagatt tgaactcttt
     1801 ccgagaaatc gcttccgtca tcgtgccgat tgtcgctttc gtggtcggta attggagtat
     1861 taccacttta tttgatggca agggtaaaat gaaagaaatc ttcatgatga tttgttattc
     1921 gctgtttccg ctcattgttt ccaaaatcct cgggatgatt ttctccaaca tgattgttgt
     1981 ggaagaagcg ggattctatc acttaatcat cggtttaggg accttcttaa tgggctatat
     2041 gattttcttt ggcttaatct ctattcatga atatggcttg tttaagtgtt tattgtccat
     2101 tttaggaacc ttgattgccg tgatggtgct cttctttgtt gtgatgcttt gctttgactt
     2161 attccaaaaa atggttggtt ttatttatac tttatatcgc gaaattgctt tacgttatct
     2221 atagaaagga ggaacaatga aggtttttaa attgaaaaat attctcgctc tgagcttgat
     2281 tgtcattatt accgtgctcc tcctcatctt ttttattgga ggactgaaag ctaaggcttt
     2341 agcgcttaac aatcttgaat ttgatttaac gggctttgta gattattctc cttatgaccg
     2401 ccaaggtaat tatgttgtgg aatacgacag tgcggctgaa gccaatgccg ccgttgcggg
     2461 cttaccggaa actagttata cgaaagttat caacgaagcg agcgacattt ataccgtcac
     2521 ctttaaatta aataaagaac gaattgttgc acaaaacaac ctctatacga tgttcttgaa
     2581 tgaagatacg acgattgtca gtgtcgctgt caacagcgaa tgtacaccga cagccgaaaa
     2641 aacggcagaa ggctttacaa attataaaaa ggccacttgt aaaacggtct ttaattccgc
     2701 ttttgattcc ggtaattcct ctgatatgaa atccaacttc attttgtact atatcggttc
     2761 caatgataaa ttaagtggaa cgggctttaa tacgcttgcc aacagtgttt tgtacaacga
     2821 tttactgtat gggacgatta aacgtcatta ccagattaaa tatgatgtgg aaaacgggat
     2881 tgaaattctc tatgaaatcg gggactttac cgtcatcaac agtttcttcc ctgaagattt
     2941 taaacgtact gatatggaag attacttccg tggcaattta ttattcttaa tcaatgccgg
     3001 ccaagttaac caaaacaaat atgaatatgc tgatttagct tatacatgga gtcaagaatg
     3061 tgcggcttat ttggaggaaa aaggcttagc gacggtgacg ccggtttttg aaggcgaaac
     3121 ttttgttgat gaggatggca atcccattcc ttctcgttat aatttaacga atattatagc
     3181 caaggatgaa gagggtcgac cgctaaacaa actcaaaatg caagccggcc ttgattataa
     3241 tgcgactaat tatggagaag aaggggctt
//
LOCUS       DAOSGN010000052         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_27635_length_3257_cov_3.308869, whole genome shotgun sequence.
ACCESSION   DAOSGN010000052 DAOSGN010000000
VERSION     DAOSGN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27635_length_3257_cov_3.308869"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..919
                     /locus_tag="PK087_01280"
     CDS             155..919
                     /locus_tag="PK087_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17933.1"
                     /translation="MLNLIGGQPKRMIMVSLGTFAVSLLVFISIGFAWISLSKSASGS
                     LVTQVGNVEAEYAFYIYLDNSRDGSSNPSLSTNECSSGSDDACYWLIENAENPYIFGD
                     TAKVFPDDRFSFAVKVKNIGDFDAHLSLDLRHLVSQGYDIADNKIQVAFEYEVTKITY
                     LVAGTESADVKTSSGITLHTGHFLKNVDDRYAIVENIPLSCTDVNTKEAIVYFDLYFD
                     PLVSGKTQLGVLTNNSNAFENQQFTISQIAVILQKQ"
     gene            983..1615
                     /locus_tag="PK087_01285"
     CDS             983..1615
                     /locus_tag="PK087_01285"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOH17934.1"
                     /translation="MFKKILIYIISTLTILLFVLSIVVLIAGSILQKEQKLINVFGYS
                     YSVVATESMEPTIMVGDIIIAKQVAFEEVQVEDIIVFYSTEYQKYFVHRVIDKDENND
                     YITKGDNPRAPIDEEPVTRDNFYGVVIKYGSFLSLGQLILKYRSLIFGVMMVIFLIII
                     ISETITIIKNINEKNRIKLENQLEQEKLKLLEEEEQRIRAEMESKRKNKN"
     regulatory      1795..1872
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1914..2564
                     /locus_tag="PK087_01290"
     CDS             1914..2564
                     /locus_tag="PK087_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17935.1"
                     /translation="MKKMKNFILIAAPLMSIVLLSVTMVLAWFVFLKTSPLATFKSGQ
                     IDVSVNINAAPIQEELAFNDLAFMDFSSDIIADDDEVLNLMASITQIKINNSLNSSDV
                     NTQLSIDPDEGLFYLLYYEGKNLTPAALDYNWHNLIVTVVGSETNVSAQRNLLATYNA
                     GVIADIASIKLEPGDWITLQIAVWGDYEELSAPSLYLSDSYDMLISIASTQWEGAI"
     gene            2564..3241
                     /locus_tag="PK087_01295"
     CDS             2564..3241
                     /locus_tag="PK087_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17936.1"
                     /translation="MVKKGFKKVFFAIMTIAMVLFVSLSLTYSWFIKTNETTYIIQAG
                     EFEASMAVAFSGNVISTGSPYYDTNKKVIIIDAGDASSTNHIENLSVSLTITPEVTAR
                     FRIKIQDEWQLKRTYYAFSDNPIIEAISHENEAIFPGNTDYPFTIANLSNYIFDSESG
                     YLYYNLLLEKGETLELEFITSGKARPVFANSAFYEECYVYLDFIVDIVQANRFSEVWG
                     ISPDYFE"
BASE COUNT         1029 a          530 c          631 g         1067 t
ORIGIN      
        1 ggaagctcgc gctcagaaaa agtatgaagc tgaattggct aaaattaaaa agcgtgaaca
       61 agctttaatt gacaaagaaa aagctatatt agaaaaggat caaaaacctc aaaacgaatc
      121 ctaagcaaat tggaaaagaa agttggtgtg atcaatgctc aatttaatag gtggtcaacc
      181 caaacggatg attatggttt cgcttggaac ctttgccgtg tctttgctcg tgtttatttc
      241 gattggattt gcctggatta gcttatccaa atcggcttcc ggttctttag ttactcaagt
      301 tggtaatgtt gaagccgaat atgcttttta tatctatctc gacaacagtc gtgatggttc
      361 atctaatccc agcctttcca caaatgaatg ttcatccgga agtgatgatg cttgttattg
      421 gttaattgaa aatgcggaaa acccttatat tttcggtgat actgccaaag tatttcccga
      481 tgatcgtttt tcctttgctg ttaaggttaa aaacattggg gattttgatg cccacctcag
      541 ccttgattta cgccatttag tatcgcaagg atacgacatc gctgataata agatacaagt
      601 ggcttttgaa tatgaagtca cgaaaattac ttatttagtt gcggggacgg aaagtgcgga
      661 tgttaaaact agtagcggta ttactttaca taccggtcat ttcttaaaga atgtcgatga
      721 ccgttatgct atagtggaaa atattccctt aagctgtacg gatgttaata ctaaagaagc
      781 cattgtttat tttgacttgt attttgatcc tcttgtcagc ggaaaaacac agcttggggt
      841 tctaacgaat aatagcaatg cttttgaaaa ccaacaattt actatttctc agattgcggt
      901 tattctccaa aaacaataat cacaaaagtt aaatcaagtc aacttgcgtg attatcgcca
      961 ttgacttttt ttgaggaaaa ttatgtttaa gaagattttg atttatatta tttccacact
     1021 cacgatttta ctgtttgttc tttcgattgt tgttctcatt gccggcagta tcttacaaaa
     1081 agaacaaaaa ttgattaatg tttttggtta ttcctattcc gtggtggcca cggaatcaat
     1141 ggaaccgacc attatggttg gtgatatcat tattgcgaaa caagtggctt ttgaagaagt
     1201 ccaagtagag gatattattg ttttttattc gactgaatat caaaaatatt ttgtccatcg
     1261 cgtcattgat aaagacgaaa acaatgatta tattaccaaa ggtgataatc caagagcccc
     1321 gattgatgaa gaaccggtaa caagagataa tttctatggt gtggttatta aatatggttc
     1381 tttcttgtct ttgggacaat tgattttaaa atatcgcagt cttatttttg gggtgatgat
     1441 ggtgattttc cttatcatca ttatttccga aacgattacg attattaaaa atattaacga
     1501 gaaaaaccgg ataaaattag aaaatcaatt agagcaagaa aagcttaaat tgttggaaga
     1561 agaagaacaa agaattcgcg ctgaaatgga atctaagcgc aaaaacaaaa attagtgctt
     1621 aataagctct ttgcaatggt tgttggaagg atttgctact gaaaatagcc aaggaataaa
     1681 tgccgtttta taattaatca tgttatttaa taacaaatta aaaatttcgc tgcatttaaa
     1741 aaaacaacaa gatattttat ttgacaacat tcttgatata tggtagaata agttgtcaat
     1801 aaagggcaaa gctaatgaaa attagcgacg caaagctata gggcctcgtc aaagtggtag
     1861 ccagttgcat tttaaatgta gctggctttt ttgtatttct aggaaggtcg attatgaaaa
     1921 aaatgaagaa cttcatttta atagctgctc ctttaatgtc aattgtgctc ttatcggtca
     1981 caatggtttt agcatggttt gttttcttaa aaacaagccc cttagcaacc tttaaaagtg
     2041 gccagattga tgttagtgtt aatattaacg ctgctcccat tcaagaagaa ttagcattta
     2101 atgatttagc gtttatggat tttagtagtg acatcatcgc cgatgatgat gaagtcttaa
     2161 atctaatggc gtccattacc caaataaaaa tcaacaacag cctaaattcc agtgatgtca
     2221 atacccaatt aagtattgat cctgatgaag gtttattcta tttactttat tatgaaggaa
     2281 agaatcttac tccggctgct ttggattata attggcataa tttaattgtt accgtcgttg
     2341 gtagcgaaac taatgtcagc gcccaaagaa atcttttggc aacttataat gccggtgtta
     2401 ttgcggatat tgcgagcatt aagttagagc ccggagactg gattacgctc caaattgctg
     2461 tctggggcga ttatgaggaa ctaagtgctc ccagcctcta cctaagtgat tcctatgata
     2521 tgctgatttc aattgcttca acgcaatggg aaggagcaat atgatggtaa aaaaaggatt
     2581 taaaaaagtt tttttcgcaa tcatgaccat cgccatggtt ctctttgtta gtttatcatt
     2641 aacctattcc tggtttatta aaaccaatga aacgacttat attatccaag ccggcgagtt
     2701 tgaagcttca atggcagtgg catttagtgg taatgtgatt agtacgggca gtccttatta
     2761 tgataccaat aaaaaagtta ttattattga tgccggtgat gcttccagca ccaaccatat
     2821 cgaaaaccta agtgtcagtt tgaccattac cccggaggta acagctcgtt ttcggattaa
     2881 aatccaagat gaatggcaat taaagcggac ttattatgct ttttccgata atccgattat
     2941 tgaagccatt tcccatgaaa acgaagctat ttttcctggg aatacggatt atccctttac
     3001 cattgcgaat ttatcgaatt atatttttga tagcgaaagc ggatatttat attacaattt
     3061 attgttagag aagggagaaa ccctagaact tgagtttatt acttccggta aagcgcgccc
     3121 cgtttttgcc aactccgctt tttatgaaga atgttatgtc tatttagact ttattgtgga
     3181 tattgttcaa gccaaccgtt tttctgaagt atggggcatt agcccggatt atttcgaata
     3241 aactaagaaa ggtgact
//
LOCUS       DAOSGN010000053         3229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_28002_length_3229_cov_2.891934, whole genome shotgun sequence.
ACCESSION   DAOSGN010000053 DAOSGN010000000
VERSION     DAOSGN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28002_length_3229_cov_2.891934"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PK087_01300"
     CDS             complement(<1..227)
                     /locus_tag="PK087_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17937.1"
                     /translation="MMNWTDFLKLIEDYTYNYSFWGFVRLGADLALTIGLLIFLYKVF
                     NMKAHVFRLLIGATVILLIFFIVLFFKLELF"
     gene            complement(224..799)
                     /locus_tag="PK087_01305"
     CDS             complement(224..799)
                     /locus_tag="PK087_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOH17938.1"
                     /translation="MNKRGAKKRFKDFVDCIKQDPHRYLYIKNMLWRLQNDGFDLNTQ
                     GYGGKTLLHLALKLNNLRLMNLFLSFGVNPNLADEQGNAPLHVAVLANQMRFIKGLVA
                     HACDINLACEQEQSPLHLAVINGNLEVVKYLIENGADIMIQDELHNYPLDYAIEEKDD
                     KILRYLLTKQEIDDERKRKILTIINKAGEKQ"
     gene            complement(792..>3229)
                     /locus_tag="PK087_01310"
     CDS             complement(792..>3229)
                     /locus_tag="PK087_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17939.1"
                     /translation="MKKMTTCLQNIDHINQKMETFKQYFTKLYLDDLDQTMNILYRHQ
                     NDDHTLTKKELKWLNQYRFQQAQQFEQVIEALSSYYDFKKQLRSNHDLFEQTTGFPAS
                     ALVLEDFQSLHDYLLDLEKRAYQDKIQFFLNHFIAVMQEDYRGMRNYLKTLELFENSH
                     RDLWKYYDKLYQYPFKGKKTTLLALFDDIKVINTYLTKAYSANDRLFAATKNTSDKFI
                     KAEEFYELDRQMDKIHQLKAYLQNHSLYKQLYQDFYQAEHSNLVAISRLLETYEVYIE
                     CFANAEALLGSLDPLANDKILGHLNRCSEVSNEISEIFTVYFKIFRDDVSKYYYDSFA
                     NNIKRMNVLLKSKDELINYLIVTDNLQVLGKYRLDKLINYIVNCQDFSHLVSDFQFTY
                     YSILKDKYLKKYAYLHNFKTLLACLNLINNNEGVIIKKNQEQLSRTMRKSTSLRFTTL
                     GLKNLDYRGYMRRTSGFKKLFLASTPILNNFLNKDDFDLIIIDDAHLLSANEYYLAID
                     GKQVILAGELPLQSSTTNNLMSRLSFATTINLNYRFSPTPLRLLNHLHNLRGCISPKV
                     NDNEGIEIINNKLTAYLLMLLKEQNDVKINLFVSSLEKQKRFYDELGEGLIKMGFSLA
                     QIIAILKNQINIVDLRLRHLLNADYNVLFLDDYRKLNNESIVMNLLDDLLLCRKRLII
                     YDHNDYLKRGQGFCLIDELNKIIDNNQIYYQEYATPLLKLLAEKLMEHGLQVYSFNHE
                     MQLLIQKDEHLFGVLLLWSDNNFSTDTLNQYRETYLWNMGLNFPVILIGTMELLTSID
                     DVIKRILSEVGHE"
BASE COUNT         1051 a          568 c          555 g         1055 t
ORIGIN      
        1 aaaaacagtt ctaatttgaa aaacaggacg ataaaaaaga ttagcaaaat cacggtcgcc
       61 ccgattaata atcgaaagac atgagctttc atattaaaga ctttatacaa aaagattaaa
      121 agcccgattg ttaatgccaa gtccgctcct aggcgtacaa aaccccaaaa tgaataattg
      181 taagtataat cttcaattag ttttaaaaaa tccgtccaat tcatcattgt ttttcacctg
      241 ctttgttaat aatggttaat atttttcgtt ttcgttcatc atcgatttct tgttttgtta
      301 ataaatagcg tagtattttg tcatcttttt cttcaattgc ataatccaag ggataattat
      361 gaagttcatc ttggatcatg atgtcggcac cgttttcgat taagtatttc acaacctcca
      421 aattgccgtt aataacggcg agatggaggg gggattgttc ttgttcacaa gcgagattga
      481 tatcacaagc atgagccacc aagcccttga taaagcgcat ttgattggcc agcaccgcta
      541 catggagggg agcattaccc tgttcatcgg ctaaattggg attgacacca aaactgagaa
      601 ataaattcat cagccgcaaa ttattaagtt tcaaagccag atgaaggagc gtcttaccac
      661 cataaccctg agtgtttaaa tcaaagccat cattttgtaa gcgccacaac atgttcttga
      721 tatagagata gcgatgagga tcttgtttga tgcagtcaac aaaatcttta aagcgtttct
      781 tggcaccgcg tttattcatg gcccacctcg cttaggattc tcttgatgac atcatcaata
      841 gaagtcaata actccatcgt gccaattaag atgaccggga aatttaaacc catattccat
      901 aaataagttt cccgatattg atttaaagtg tcagtcgaaa aattattgtc cgaccacagt
      961 aataagaccc caaaaagatg ctcatccttt tgaattaaaa gctgcatttc atgattaaaa
     1021 ctataaacct gcaagccatg ttccatcaac ttttccgcta acaactttaa caacggagtg
     1081 gcatattctt gataataaat ttggttgttg tcaatgattt tgttaagttc atcaatcaag
     1141 caaaaccctt gaccacgttt taaatagtcg ttgtggtcat aaataatcaa tctttttcta
     1201 caaagcaata aatcatcaag tagattcata acgatggatt cgttattcaa cttgcgataa
     1261 tcatctaaaa acaagacatt gtaatcagca ttaagcaaat gccttaagcg taaatcaaca
     1321 atgttaattt ggtttttcaa aatggcaata atttgggcta atgaaaagcc cattttaatt
     1381 aaaccctccc ccagttcatc ataaaagcgt ttctgttttt ccagtgacga cacaaaaagg
     1441 ttgattttta catcattttg ttcttttaaa agcattaata aataagccgt taatttatta
     1501 ttgattattt caattccttc attatcgtta actttcgggc tgatgcaacc tcgcaaattg
     1561 tgaagatggt ttaataagcg aaggggcgtc ggcgaaaagc gataattaag attaatggtt
     1621 gtcgcaaacg aaagtcgtga catcaaatta ttggtcgtgg agctttgcaa tggtaattca
     1681 ccggcaagga tgacttgttt accgtcaatt gctagataat actcattggc acttaataaa
     1741 tgagcatcat cgataatgat taaatcaaaa tcatctttgt ttaagaagtt gtttaaaatg
     1801 ggagtggaag ccaaaaacag ttttttaaaa ccgcttgtcc gccgcatata gccccgataa
     1861 tcaaggtttt tcaaaccaag ggttgtaaat cgcaggctgg tgcttttgcg catggtccgc
     1921 gatagctgtt cttgattctt tttgataatc acaccttcat tattgttgat aagattcaag
     1981 catgctagca gcgttttaaa attatgcaag taagcatatt tctttaaata cttatctttc
     2041 aagatggaat aataagtaaa ctgaaaatcg ctaactaaat gcgaaaagtc ctgacagttg
     2101 acaatataat taatcagttt gtccagccgg tactttccca aaacttgtaa attatcagtc
     2161 acgattaaat aattaatcaa ttcatcttta gactttaaaa ggacattcat ccgtttaata
     2221 ttgtttgcga aagaatcata atagtatttt gaaacatcat cgcggaagat tttaaaataa
     2281 acggtaaaga tttcgctgat ttcgttgctt acttcactgc aacggtttaa atgacctagg
     2341 attttgtcat tagcaagggg atcgagactt cctaacaagg cttccgcatt tgcgaaacat
     2401 tcgatgtaaa cttcataagt ttccaacaaa cggctaatgg ctaccaagtt ggagtgttcg
     2461 gcttggtaaa agtcctgata caattgttta tacaaagaat gattttgtaa ataagccttt
     2521 aactgatgaa ttttatccat ctgtcggtca agttcataaa attcttcagc tttaataaat
     2581 ttatcacttg tatttttggt agctgcaaat aaacgatcat tggcggaata ggctttggtt
     2641 aaataagtat taatgacttt gatatcatca aagagcgcta aaagggtggt ttttttgccc
     2701 ttgaagggat actgatatag tttatcataa tatttccata aatcccgatg tgaattctca
     2761 aataattcca gcgtttttaa ataattgcgc ataccccgat aatcttcttg cataacggca
     2821 ataaagtggt ttaagaaaaa ctggatttta tcttggtaag cccttttttc caaatcgagc
     2881 aagtaatcat gtaaactttg gaaatcctct aaaactaaag cagaggccgg aaacccggta
     2941 gtttgttcaa acaaatcgtg gttgctgcgc aattgcttct tgaaatcata ataagaagac
     3001 aaagcttcaa ttacttgttc gaattgttgc gcttgttgga aacgatattg atttaaccat
     3061 tttagttctt ttttcgttaa agtatggtca tcattttgat gacgatataa aatattcatc
     3121 gtttggtcta aatcatctaa ataaagttta gtaaaatact gtttgaaggt ctccatcttt
     3181 tgattgatat gatcgatgtt ttgcaaacaa gttgtcattt tcttcataa
//
LOCUS       DAOSGN010000054         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_28180_length_3215_cov_3.936709, whole genome shotgun sequence.
ACCESSION   DAOSGN010000054 DAOSGN010000000
VERSION     DAOSGN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28180_length_3215_cov_3.936709"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PK087_01315"
     CDS             <1..867
                     /locus_tag="PK087_01315"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HOH17940.1"
                     /translation="NFADGNHSVWYQESLFVGYRYYETFKKAVRYPFGYGLSYTTFSY
                     TDLQVSIPEIRVSGPIEVKVKVKNTGKRPGSEVVMLFVQNNTSPVFKPLRELRAFEKV
                     YLEPNEQKEVVFVLNYQDFAYYDVDMKTYHVDRGKYKIQICKHASKVILEKTVTKQDN
                     EIGFNSPYPSAYNDKEYHIEKEDFQKLLGRLLPPKSIIKHRPYTMDSTLEDVKHTILG
                     FIMYRVVLRKAAKLSKRSSNDWMRQAMKKTLAQTPLRVIATMSGGAISLIQMEGLVDL
                     INFRIIRGLGKI"
     gene            864..2513
                     /locus_tag="PK087_01320"
     CDS             864..2513
                     /locus_tag="PK087_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012575803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOH17941.1"
                     /translation="MRWYKSSVIYQIYPRSFQDSNHDGIGDLPGIISRLDYLQHLGVT
                     AIWLSPIYASPLYDNGYDIADYCAIHPDYGTMADFQKLLAEAHRRQIKIIMDLVINHT
                     SSEHSWFKQAISDEKSPYRDYYFIRSGKKKPNNWTSFFGGSAWTYLPNENLSYLHLFA
                     PEQPDLNWENPQLRMEIKNILRFWLDMGVDGFRCDVINLLSKPKKLRNSRRFLVLKGA
                     EYYINGPKIHEYLQELKRDVFNQYDMMTVGECVFITPAKALEYIEEGVDELNMVFQFD
                     HMAVDNYFKWFLRPFKPYRLKLALAKWQDAINGRGWNSLYLENHDQPRSVSRFGNLDY
                     YYASATMLATLVFFQQGTPFIYQGQEIGMTNAHFTSLNQYRDVETKRIYQIGRTQLKL
                     SHKRMMHKIKYMSRDNARTPMQWDDSKHGGFSDANPWIEANPNFVTINVKDNLANEHS
                     IFHYYQKLIALRKTYSVIIEGDFKLHYPKHRALVYYERCFQDQQLIVLCNYSTKPLSF
                     KPLHDFSEFTVILDNDDNKGPVLQAYQARVLFRRTADDKHS"
     gene            2497..>3215
                     /locus_tag="PK087_01325"
     CDS             2497..>3215
                     /locus_tag="PK087_01325"
                     /inference="COORDINATES: protein motif:HMM:NF028185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HOH17942.1"
                     /translation="MISIRDHLFHLQTKQTSYVFSVEHDLLIHHYYGANIVFENINHL
                     TTKLRGGAGTAVEYEGKPQVYVDRLPLEYALFGTGDFREPALLLHNPSGGFYSNFKFD
                     RYEINPKLLGPLPEARQADDVLMIILKDESRQLELHLVYKVFEKANIISRYARLINNS
                     DQVVIVDRFFSGQLDLERNQLEIMTFDGAWAKERHVHIHRLEGGIFINDSKCGASSNR
                     HNPLTILKDPQTTEEAGEAFG"
BASE COUNT          969 a          621 c          660 g          965 t
ORIGIN      
        1 aattttgcgg acggcaacca cagtgtttgg tatcaagaaa gcctctttgt cggctatcgt
       61 tattatgaaa cctttaaaaa ggcggttcgt tatccctttg gttacggctt aagttatacg
      121 actttcagtt atacggattt acaagtttcg attccggaaa ttcgtgtttc cgggccgatt
      181 gaagttaaag tcaaagtgaa gaacacgggc aaacgtccgg gaagtgaagt cgtcatgttg
      241 tttgtacaaa acaacacttc gcccgttttc aaaccgctac gcgagttaag ggctttcgaa
      301 aaagtttatt tggaaccgaa tgaacaaaaa gaagttgttt ttgttctcaa ttatcaggac
      361 tttgcttatt atgatgtcga tatgaaaact tatcatgtcg accgcgggaa atataaaatc
      421 caaatatgca aacatgcttc caaagttatc ttggagaaaa cggtcacaaa acaagacaat
      481 gaaataggct ttaattctcc ctatccgagc gcttacaatg ataaagaata tcatattgaa
      541 aaagaagatt ttcaaaaatt attagggcgc ttattaccgc ctaaaagcat tatcaaacat
      601 cgtccctata cgatggattc caccttggaa gatgttaaac atacgatttt aggttttatt
      661 atgtatcgcg ttgttttgcg gaaggcggcg aagctaagca agcgttcaag caatgattgg
      721 atgagacaag ccatgaaaaa aacacttgcg caaacaccgc taagagtcat tgctacgatg
      781 agtggtgggg caatttcgct catccaaatg gaagggctag ttgacctcat caattttaga
      841 ataattcggg ggttaggaaa gatatgagat ggtataaatc gagtgttatc tatcaaattt
      901 atccacgcag ttttcaagat tcaaaccatg atggaattgg tgatttaccg ggaatcatta
      961 gccgtttaga ttatttacaa catttagggg taaccgccat atggttatcc cctatttacg
     1021 cttctcccct ctatgacaat ggctatgata ttgctgatta ttgtgccatt catcccgact
     1081 atggaacaat ggccgatttt caaaaacttt tggcggaagc acatcgtcgc cagattaaga
     1141 ttatcatgga tttagttatc aaccatactt ccagtgaaca ttcgtggttt aaacaagcga
     1201 ttagtgatga aaaaagtccc taccgcgatt attattttat tcgttcgggg aaaaagaaac
     1261 ccaacaactg gacttccttt tttggcggtt cggcttggac ctatcttcct aacgaaaatc
     1321 tttcttattt gcacttgttc gcccctgaac aacccgacct taattgggag aatccgcagc
     1381 ttcggatgga aatcaaaaac atcctccgtt tttggttgga tatgggggtg gatggtttcc
     1441 gttgtgatgt tattaatctt ttatcaaaac caaaaaaact tcgcaacagt cgccgttttt
     1501 tagttttaaa aggagcggaa tattatatta acggaccgaa aattcatgaa tatttgcagg
     1561 aattaaaacg cgatgttttt aatcaatacg atatgatgac ggtcggagaa tgtgttttca
     1621 ttacacctgc aaaggctttg gaatatattg aagaaggcgt cgatgaactg aacatggtgt
     1681 ttcagtttga tcatatggct gttgataatt actttaaatg gtttttacgg ccctttaaac
     1741 cgtatcgttt aaaacttgct ttagctaaat ggcaggatgc cattaatggt cgcgggtgga
     1801 attccctcta tttggaaaac cacgaccaac cgcgttccgt cagtcgtttt gggaatcttg
     1861 attattatta tgccagtgcg acgatgcttg caaccctcgt tttcttccaa caaggaacgc
     1921 cttttattta tcagggccaa gaaatcggga tgaccaatgc ccacttcacc agtttaaatc
     1981 aataccggga tgtggaaacc aagcgtattt accaaatcgg tcgcacgcaa ttaaaattat
     2041 cccacaaacg aatgatgcat aaaattaaat acatgagcag ggataatgcc agaacgccga
     2101 tgcagtggga tgattctaag catggcggtt tttcggatgc aaacccatgg attgaagcta
     2161 accccaattt tgtgacgatt aatgtaaaag ataatctagc caacgaacat tccattttcc
     2221 attattatca aaaattgatt gccttaagaa aaacatattc agttattatt gaaggtgatt
     2281 ttaaactgca ttatcccaaa catcgggctt tagtttatta tgaacgatgt ttccaagatc
     2341 aacaattaat tgttttatgc aattattcaa ccaaaccctt aagctttaaa ccgctgcatg
     2401 acttttccga attcacggtt attcttgata atgatgacaa taaagggcct gttttacaag
     2461 cctatcaagc tcgggtcttg ttcaggagga ctgccgatga taagcattcg tgatcatctt
     2521 ttccacttac aaacaaagca aacatcctat gttttttccg tcgaacatga tttattaatt
     2581 catcattatt atggtgccaa catcgttttt gaaaatatta atcatcttac caccaaacta
     2641 aggggtgggg cggggacagc ggttgagtat gaaggaaagc cccaagtata tgtggatcgt
     2701 ttacctttag aatatgcctt gtttggcacc ggtgattttc gggaaccggc tttgttatta
     2761 cataatcctt caggcggttt ttacagcaat tttaaatttg atcgctatga aattaatcct
     2821 aaattgcttg gtccgctgcc agaagccaga caagcggatg atgtgcttat gataatctta
     2881 aaagatgaaa gccggcaact ggaattacac ttggtttata aagtgtttga aaaggccaat
     2941 atcatcagcc gttatgcgcg cttaatcaat aattcggatc aagtcgtgat tgtcgatcgc
     3001 ttcttttccg ggcaactgga tttggaaaga aaccaattgg aaattatgac ctttgacggt
     3061 gcttgggcta aagagcgcca tgtgcacatc caccgccttg aaggcggcat cttcatcaat
     3121 gattccaaat gcggggccag cagcaaccgc cataatccgt taacgatttt aaaagatccc
     3181 cagacaaccg aagaagcggg tgaagctttc ggttt
//
LOCUS       DAOSGN010000055         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_28482_length_3192_cov_1.962066, whole genome shotgun sequence.
ACCESSION   DAOSGN010000055 DAOSGN010000000
VERSION     DAOSGN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28482_length_3192_cov_1.962066"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PK087_01330"
     CDS             <1..267
                     /locus_tag="PK087_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOH17943.1"
                     /translation="YVIDQEGLKYIDYDLDVKVFPDGEVLLLDRDEYDFNTKVMQYPE
                     EIKGIIKENLTKLMEFIENKKDPFNRACVNGWYELFMKTTQEKN"
     gene            282..950
                     /locus_tag="PK087_01335"
     CDS             282..950
                     /locus_tag="PK087_01335"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOH17944.1"
                     /translation="MIISESWDGKINIEFYQPYFLKLVRFLEREYANYRVFPPKHELF
                     NAFTLTPYENVKVVILGQDPYHEEGQAHGLCFSVKNNVPFPPSLQNIFRELKNDLGIS
                     YPKSGDLTKWAQQGVLLLNAVLSVRQGFATSHKDMGWEQFTDFILKLINDKQEPVVYI
                     LWGKYAINKAALLTNPHHLIITGPHPSPLSAYRGFFGGKYFSKCNQFLASQGIKKIDW
                     NLNY"
     regulatory      981..1083
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1109..1642
                     /locus_tag="PK087_01340"
     CDS             1109..1642
                     /locus_tag="PK087_01340"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOH17945.1"
                     /translation="MTKDIKAITETAILVALGIVLQIISNLVAIFQMPQGGSVSFAYL
                     PILAIGLRRGLKYGFLGGLIFAFSHWLLDGYVLHFGSIFFDYLFVGLAFGLTGFFHQK
                     RTNTFWFLLALFLGGFVKYIMHSLSGVMFFANNTPADQTIFIYSFIIYNLPYNAISTV
                     LAMVLGLSMKTIITDVH"
     gene            complement(1671..2243)
                     /locus_tag="PK087_01345"
     CDS             complement(1671..2243)
                     /locus_tag="PK087_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HOH17946.1"
                     /translation="MKVKYPNPSIKIEKPNKSKSHGMLFENGLNISNEYYRIHNRAII
                     YKKPTPIQVVKVEYPARNKARIAEAFYRVPSTTDYNGIYRGQYLDYEAKETNNLSFAF
                     KHIYPHQIEHLTKVAEMGGIAFIIIFYRKVNEIYLIDIKVFNELVQLGEKGGLKSIPV
                     EVAKAKGQLVTQGYTPPIDYLAAVDLIYFK"
     gene            2410..>3192
                     /locus_tag="PK087_01350"
     CDS             2410..>3192
                     /locus_tag="PK087_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006907667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOH17947.1"
                     /translation="MNIKQNDLNERQKFIRNFSIIAHIDHGKSTLADRILELTHTVEA
                     RLMKEQLLDSMDIERERGITIKLNAVEIVYQALDGHRYILHLIDTPGHVDFTYEVSRS
                     LAACEGAILVVDAVQGIEAQTLANVYLAIDNNLEIIPVINKIDLPNANPEFVKKEIEN
                     VIGLDAKDALLVSAKQGLGIREVLESIVKKVPCPQGDPHAPLQALIFDSYYDQYRGIV
                     PSIRIINGTVRKGDKIISMANGVEFEVTEVGVYTPNEVKREFL"
BASE COUNT          968 a          547 c          586 g         1091 t
ORIGIN      
        1 tatgtcattg atcaagaagg tttaaaatac attgactacg atttagatgt taaagttttc
       61 cctgatggtg aagttttgtt gcttgatcgt gatgaatatg attttaatac gaaagttatg
      121 caataccccg aagaaataaa gggcatcatt aaagaaaacc ttacgaaatt aatggaattt
      181 atcgaaaata aaaaagaccc ctttaatcgt gcttgtgtca atggatggta tgagctgttt
      241 atgaaaacca cccaggaaaa aaattaagtt ggtgaaaaat catgattatt agcgaaagtt
      301 gggatgggaa aattaacatc gaattttacc aaccttattt tttaaaatta gtgcgttttt
      361 tggaaagaga atatgcaaat tatcgcgtct ttcctccaaa gcacgaattg tttaatgctt
      421 ttactttgac tccttatgaa aatgtaaaag tcgtcatttt gggtcaggat ccctatcatg
      481 aggaaggaca agctcatggc ttatgttttt ctgttaaaaa taatgttcct tttcccccct
      541 ccttacaaaa catttttcgc gaattgaaaa atgatttggg aatttcttat cctaaaagtg
      601 gtgatttaac gaaatgggct caacaaggcg ttttactttt aaatgccgtg ttatcagttc
      661 gccaaggttt tgcaacgagc cataaagata tgggatggga acaatttacg gacttcatct
      721 taaaattaat caatgacaaa caagaaccgg ttgtttatat tctttggggg aaatatgcaa
      781 ttaataaggc ggccctcttg accaatcccc atcatcttat catcacgggt ccgcatccca
      841 gccccctttc ggcttatcgt ggtttttttg gtggcaaata cttttccaaa tgcaatcaat
      901 ttttagcgtc ccaagggatt aaaaaaattg attggaatct taattattaa aaggctggca
      961 ttttttataa atttgtaata taatcattaa tggggagctt cggctgagag gttgcgtcag
     1021 ggcaacgacc cttaaacctg atctaggtaa tgctagcgta ggaaaccttt tttaacgagc
     1081 attcctagaa ttactctagg agttttttat gacaaaagac attaaagcga ttacggaaac
     1141 agcaatattg gtggctttgg gaatcgtctt gcaaatcatt agtaatttag ttgcgatttt
     1201 tcaaatgcct caaggtggta gtgtttcttt tgcttatcta cccattttag ccattggttt
     1261 aagaagaggt ctaaaatatg gttttttagg cggtttaatc tttgctttta gtcattggtt
     1321 acttgatgga tatgttttac attttggctc tattttcttt gattatttat ttgttggttt
     1381 agcttttgga cttactggtt tttttcatca gaaacgaacc aacacttttt ggtttctttt
     1441 agccttattc cttggtggtt tcgtgaagta tatcatgcat tctttaagcg gagttatgtt
     1501 ttttgccaat aatactcctg ctgaccaaac aatttttatt tattctttca tcatttacaa
     1561 tttaccatat aatgccatat cgacggtttt agccatggtg ttgggtttat cgatgaaaac
     1621 aattattact gatgtccatt aaaaaagtct tgctacgcag caaggctttt ttacttaaaa
     1681 taaattaaat caacggcagc caaataatca atcggcggtg tataaccttg agtaactaat
     1741 tggcctttag ctttggcgac ctcaacagga atgcttttta aaccgccttt ttctcccaat
     1801 tgtaccaatt cattaaaaac tttaatgtca attaaataga tttcattgac ttttcgataa
     1861 aaaataataa tgaaagcaat cccccccatt tccgcaacct tagttaaatg ctcaatttga
     1921 tgagggtaaa tgtgcttgaa ggcaaaactt aaattattgg tttctttggc ctcataatca
     1981 agatattgac cacgataaat cccattataa tcagtggttg aagggacacg gtaaaaagct
     2041 tccgcaatcc tggctttgtt gcgggcgggg tattcaactt tcacaacctg aatgggagtt
     2101 ggtttcttat aaatgatggc acgattatgg atgcgataat attcgttaga gatattaaga
     2161 ccattttcaa acagcattcc atgggacttg cttttgtttg gtttttcaat tttgatgctt
     2221 ggattgggat attttacctt cataaatcct tgttttcctt tttcatgcct ttattatagc
     2281 attttatctt tattttttca ttaattctcg gataatcttt tttgagaaaa ctcaaaccaa
     2341 gccgtttttt aattatattg cctttttttg cgaaataagg tagaataatt actataggaa
     2401 atgaaaaata tgaatatcaa gcaaaacgat ttaaacgaac gacaaaaatt catccgcaat
     2461 ttttcgatta ttgcccatat tgatcatggt aaaagcacat tagccgaccg cattttggaa
     2521 ttaacacata cagtggaagc gaggttaatg aaagaacaat tgcttgattc catggatatt
     2581 gaacgagaac gcgggataac gattaaatta aatgcagtgg aaattgttta ccaagcttta
     2641 gatggccatc gttacatttt acacctaatt gacacaccgg gacatgtcga ctttacctat
     2701 gaagtttccc gttcattagc agcttgtgaa ggtgctattt tagttgttga tgccgttcaa
     2761 ggaatagaag cccaaacatt agccaatgtt tatctagcca ttgataataa cttagaaatc
     2821 attcccgtta tcaataaaat tgatcttccc aatgctaatc cggaatttgt aaaaaaggaa
     2881 attgaaaatg tcattggttt ggatgccaaa gatgccttgt tagtttccgc aaaacaagga
     2941 cttggcattc gtgaagtttt agaaagcatt gtaaagaaag tcccttgtcc ccaaggtgac
     3001 ccccatgctc ctttacaagc cctaatcttt gattcctatt atgatcaata tcgcgggatt
     3061 gttccctcca tccgcatcat taatggcacc gttcgtaaag gcgataaaat tatctccatg
     3121 gccaatggtg tagaatttga agtgacggaa gtcggcgttt atactcctaa tgaagtcaaa
     3181 cgggaattct ta
//
LOCUS       DAOSGN010000056         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_28559_length_3185_cov_2.988498, whole genome shotgun sequence.
ACCESSION   DAOSGN010000056 DAOSGN010000000
VERSION     DAOSGN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28559_length_3185_cov_2.988498"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /locus_tag="PK087_01355"
     CDS             <1..1967
                     /locus_tag="PK087_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOH17948.1"
                     /translation="ATMEGGGYIISGHGTKKDQLMNVKVGDQVTIDWKTKKIIIARDP
                     LLSSQFLAHRFLQTATVEYEHAIAVGYRFDEVFVNQLLELNREDQLVIDEIVGKENPT
                     ASEINQVSQLAKDIEARALSIKYLTSYSQTTEIRALWHRPNAASLKEDNLANLQAFVQ
                     EVKSLGFNTLFVETLWNGYVSYRSELMSTHPQVASFTYGEEYQNDYIAALIGECRKVG
                     IDVHAWCHTFNGGDASYKAASIKDEWLLEDYQGLTLHPNVYGGSYFLDPSNPEVLTFL
                     TNVFSEMIEKYDFAGLQLDYIRYDDNNYAQSPIRDSGYGLLPETRFKEAYGLTGDVRT
                     LIKQSANLAKWNQWRQNNITNAVKVFSQTLRRLKPDIIISADVVADINNAKATCMQDW
                     QTWVKRGYLDLLCPMIYTGSADVVEKDSLAIMKQMKNLSFLASGIAPTYYGYSLEINH
                     DQFIATRAADGVAFFASQNVVGLADVSASIKTGVFRYPALSPLVKTSLVINHGLIGLK
                     QYVQTAMINEAKQTMFLNYVDEMIEMDCENPADYQKLMEKMLDVQTMASAINSNFQRD
                     FFVQELGHLIHILDVKISREMMAFGYYDPSDGERLDPSLFSYPKVKTEPEPEPEPETE
                     RGCLKIFTAPVWLFWGALMLLFRKKERGSI"
     gene            1964..2923
                     /locus_tag="PK087_01360"
     CDS             1964..2923
                     /locus_tag="PK087_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17949.1"
                     /translation="MSLAIRVIPTLDSHYVDSSKFQKVPVYYGKIENEIPAPPVPECF
                     LGAWYRKVFSSTDYWLGIEGIIKLGEFIPDKARFNLDGKGRYMDNPSIYMGGKSAKES
                     DAGLNLNLSYSSSDTKEDLSLSSPKLAYRPFWRYIYNSTTDFSGNVDRQEINSWNVHN
                     PRHLSNYYFPGDVIKMSVYSPLKDYLQLRIDVIEATSNPKYVKIRMGYGLENNLPTSF
                     LSPLFFSKGHGYEKAEFKRVNSIDQYGNEGLVAQNTNAEVTEALWQEVYLYREINGEL
                     VKVPFLQNRQTSMICPHQDVITVKKHPLDPTGEAIIIHPGRKN"
     gene            2934..>3185
                     /locus_tag="PK087_01365"
     CDS             2934..>3185
                     /locus_tag="PK087_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOH17950.1"
                     /translation="MKKIVYLPLDERPCNYGFPSEIFNSSLYSIVVPPKDIMGEKKKP
                     AKHEALEAFLLEECRDADGLVISLDTLIYGGIVPSRLHYL"
BASE COUNT          952 a          582 c          683 g          968 t
ORIGIN      
        1 aggcgacgat ggaaggagga ggatatatca tttcagggca cggaactaaa aaagaccaat
       61 taatgaatgt gaaagtaggt gaccaagtca ccattgattg gaaaaccaaa aagattatta
      121 ttgcgaggga tcccttacta agctcgcaat ttttagctca tcgtttctta caaactgcca
      181 ctgtcgaata cgaacatgcc attgcagtag gatatcgttt tgatgaggtt ttcgttaatc
      241 agctgctaga attaaaccga gaggatcaac tggtaatcga tgaaatcgtc ggaaaagaaa
      301 atccaaccgc ttctgaaatt aaccaagtta gccaactagc caaagatatt gaagctcgag
      361 ccttgtcgat taaatattta acctcctatt cgcaaaccac cgagattcgc gctctttggc
      421 atcgtcccaa tgcggcgagt ttaaaggaag ataatttagc gaatttacaa gcctttgtcc
      481 aggaagtaaa aagcctcggc tttaatacct tatttgtaga gacgctttgg aatggctatg
      541 taagttaccg ttcggaatta atgagtaccc atccgcaagt agcgagcttt acttatggtg
      601 aagagtatca aaatgattat attgctgctt taatcggtga gtgccggaaa gttggcattg
      661 atgttcatgc ctggtgccac acctttaatg gcggtgatgc aagttacaaa gcggcctcca
      721 tcaaggatga atggttattg gaagattatc aaggtttgac cttacacccc aatgtttatg
      781 gagggagtta ttttttggat ccttccaatc ctgaagttct tactttctta acaaatgttt
      841 tttcggaaat gattgaaaaa tatgattttg caggtcttca gcttgattat atccgttatg
      901 atgacaataa ctatgcgcaa agtccgattc gggatagcgg gtatggatta ttaccggaaa
      961 cgaggtttaa ggaagcttat ggattaacgg gtgatgtacg gactttaatt aaacaaagtg
     1021 ccaacttggc aaaatggaat caatggcgcc aaaacaatat taccaatgcc gttaaagtgt
     1081 tttcccaaac tttgcgtcgc cttaaacctg atattattat ttccgccgat gttgtagctg
     1141 atattaataa tgcgaaagcg acatgtatgc aagattggca aacttgggtt aagcgaggtt
     1201 atttggactt attatgtccg atgatttata ccggcagtgc ggatgtcgtt gaaaaagatt
     1261 ccttagcgat tatgaagcaa atgaaaaact tatcgttttt agcatccggc attgcgccta
     1321 cttattatgg atattcttta gaaatcaatc atgatcaatt tattgccacg agagccgctg
     1381 atggtgtcgc cttttttgcc agtcaaaatg tcgtgggctt ggcggatgtt tccgcatcca
     1441 tcaaaaccgg ggtctttcgc tatccggcgc tttcaccctt agttaagacc agcttggtga
     1501 ttaatcatgg cctcattggt ttaaaacaat atgttcaaac agcgatgatt aatgaagcta
     1561 agcaaacgat gtttttaaat tatgtcgatg aaatgataga aatggattgt gagaacccgg
     1621 cagattacca aaaactgatg gaaaagatgc tcgatgtaca aacaatggct tctgctatta
     1681 attctaattt ccaaagggat ttttttgttc aggaattagg gcacttaatc catattttag
     1741 atgtgaaaat cagtcgggaa atgatggctt tcggctacta tgacccaagt gacggcgaac
     1801 gattggatcc tagtttattt tcatatccca aagtgaagac cgaaccagaa cctgagcccg
     1861 aaccggaaac tgaacgcgga tgtttaaaaa tatttacggc tccggtgtgg ctgttttggg
     1921 gggcattgat gttattattc cgaaaaaaag aaagaggtag tatatgagtt tagccattag
     1981 ggtcattccg actttagaca gccattatgt tgatagcagc aagtttcaaa aagtccccgt
     2041 ttattatgga aagattgaaa acgagattcc tgctcctcct gtgccggaat gttttttagg
     2101 cgcctggtat cgtaaagttt tttcgtcaac agattattgg ttaggaattg aaggcattat
     2161 caaattgggg gaatttattc ccgataaagc ccgtttcaat cttgatggga aaggccgtta
     2221 tatggacaat ccgtccattt atatgggtgg aaaaagtgct aaagaaagtg atgccggcct
     2281 caatttaaat ttatcttatt catcatccga tacgaaagag gacttaagtt tatcttcacc
     2341 gaaactcgct tatcggcctt tttggcgtta tatttataat tccacaaccg atttttccgg
     2401 aaatgtggat cgtcaggaaa ttaattcttg gaacgttcac aatcctcgtc atttaagcaa
     2461 ttattacttt cccggtgatg ttattaaaat gagtgtttac agtccgttaa aagattattt
     2521 acaactacga attgatgtta ttgaagcgac aagtaatcct aagtatgtga aaatccgcat
     2581 gggctatggt cttgagaaca atttaccgac atctttttta agtccccttt tcttctccaa
     2641 aggtcatggt tatgaaaaag ctgaattcaa aagagttaat tcgattgatc agtatggcaa
     2701 tgaaggtttg gttgcgcaaa acacgaatgc ggaagtgacc gaagccctct ggcaagaagt
     2761 ttatttgtat cgagaaatta acggtgagct tgtgaaagta ccctttctac aaaatcgtca
     2821 aacatccatg atttgtcccc atcaagatgt cattaccgtt aagaaacatc cgcttgatcc
     2881 taccggagaa gccattatca ttcatccggg acgaaagaat taaggagtta ataatgaaaa
     2941 aaattgttta tcttccgctt gacgaacgac cttgtaatta tggctttccg tcagaaatct
     3001 ttaacagttc tctttattcc attgttgttc cgcctaaaga tattatgggc gaaaaaaaga
     3061 agccggccaa gcatgaggct ttagaagcct ttttacttga ggaatgccgt gatgccgacg
     3121 gtttggtgat ttcccttgac accttaattt atggtggcat tgttccttca cgcttacatt
     3181 attta
//
LOCUS       DAOSGN010000057         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_28639_length_3178_cov_3.708293, whole genome shotgun sequence.
ACCESSION   DAOSGN010000057 DAOSGN010000000
VERSION     DAOSGN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28639_length_3178_cov_3.708293"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1148
                     /locus_tag="PK087_01370"
     CDS             237..1148
                     /locus_tag="PK087_01370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH17951.1"
                     /translation="MKKISLIIPCYNEEAVLQTTYNEIIKHLPDHYSYQFILVDDGSQ
                     DKTLKVMKDLAAQDARIKYLAFSRNFGKEAALLAGLEAAKKINSDAVIILDADLQDPP
                     SLIPEMIDYYEQGYQHIYTKHRSRAGESKIKTFWALSFYKVYAFLTKNKSLAKGARDF
                     CLLDRQAIDALLAVKDYKRFTKGLYNWIGFEKKCLEFDYVPRAKGTTKWSFKSLFRYA
                     SRGIKQFSSVYLWVTTLLMLLAFGLVVFDIVNGIIKQWDWLSLRIDGLMFFVFLALHT
                     ILKLLYDIRDQGLNRPIYLTKESNLDD"
     gene            1141..1569
                     /locus_tag="PK087_01375"
     CDS             1141..1569
                     /locus_tag="PK087_01375"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOH17952.1"
                     /translation="MINFINKFLTRNFVVFCIIGVINTLIHWGVYLLLYQKSVLIANT
                     LAFIVASLFSYWANATFTYHEKTTSKTFMLSFLTFLGKLVLSDSFAFLFELWFKTMNW
                     PLLIKLIPFFVTMILLPMQFLVFNRIFKKPRPEEEKIEKM"
     gene            complement(1611..2870)
                     /gene="tyrS"
                     /locus_tag="PK087_01380"
     CDS             complement(1611..2870)
                     /gene="tyrS"
                     /locus_tag="PK087_01380"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOH17953.1"
                     /translation="MNLLEDLKWRGLVYDIINEEELSKRLEEAPITLYCGFDPTADSL
                     HVGSLLPIVTLMRFYRAGHRVIALTGGGTGLIGDPSGKRNERQMNEVATVHKWGALFK
                     KQFARFLVFDGERAFCVDNYEWLSKLSAIDMCREFGKHFNINQMLAKESVKSRLDSGL
                     TYLEFSYMILQSIDFLHMYQDANLHCEMQIGGQDQWGNITAGMDLIRKVVGSEAKVFG
                     LTLPLITKSDGTKFGKTESGAIWLDEDKTSPYEMYQFFINTADADVIRFLKYYTFLSH
                     EQILDLEEKVTREPHLRAAQKVLAKEIVCLVHGEEAYNQAIAMSEALFSGQIGSLTAK
                     EISVGFSDVPSLAISEDLGLIETLVLVKAASSKREARELIQNKAISINGEIISSLEHI
                     VRKAQAIERQFTVIRRGKKNYYLIKHQ"
BASE COUNT          922 a          653 c          555 g         1048 t
ORIGIN      
        1 tccatcaata aaaagtcctt tattgattat tagagggagt gtgattatgc taaagataat
       61 taaaaagccg aacgaatatt gccaataaag cttgtttttc ccttttttaa tcataaaaaa
      121 ataaaatcct taaatagcat tctaacatag ttatggttct tattcaatta tttatttgct
      181 tgttcctagc aattatagta taatcaaaat tacaaatcta aaaaaaggac atttttatga
      241 agaaaatttc gcttattatc ccttgttata acgaagaagc cgttttacaa acaacttata
      301 acgagattat caagcacctt cccgaccact attcctatca attcattctt gtggatgacg
      361 gttcccaaga taagacctta aaggtgatga aggacttagc cgcccaagat gcccgcatca
      421 agtatttggc tttttcccgc aacttcggaa aagaagccgc tttattggcc ggtttggaag
      481 ccgctaaaaa gattaacagc gatgcggtca tcattcttga tgccgattta caggatccac
      541 catcattaat tcctgaaatg attgattatt atgaacaggg ttatcaacat atctatacta
      601 aacaccgcag ccgtgccggc gaatccaaaa ttaaaacttt ttgggctttg tcgttttaca
      661 aagtttatgc ctttttaacg aagaataaat ccctagccaa gggcgccaga gatttttgtt
      721 tgcttgaccg ccaagccatt gatgctcttt tagccgttaa agattataaa cgctttacca
      781 agggtttata taattggatt ggttttgaaa agaaatgtct ggagtttgat tatgtgccgc
      841 gggctaaagg cactaccaaa tggagtttta aatccttatt tcgctatgca agccgcggca
      901 ttaaacaatt cagttcggtt tatttgtggg taacaacttt attaatgctc ttggcttttg
      961 gactggttgt ctttgatatt gtcaatggga ttatcaagca atgggattgg cttagtttac
     1021 ggattgacgg cttaatgttc tttgtctttt tagctctcca tactatttta aaattgttgt
     1081 atgatattcg tgatcaaggc ttgaaccgac cgatttatct aactaaggaa agcaatcttg
     1141 atgattaatt ttatcaataa atttttaacg cgcaactttg ttgtcttttg tattatcggt
     1201 gttattaata ccttaattca ttggggcgtt tatttacttt tgtatcaaaa atccgtgtta
     1261 atagccaata ccttggcgtt cattgttgct tcgctttttt cttactgggc gaatgccact
     1321 tttacatatc acgaaaaaac cacttccaaa accttcatgc tttccttcct aactttttta
     1381 ggaaaattag tgctttccga tagtttcgcc tttttatttg aactgtggtt caaaacgatg
     1441 aattggccgt tgttaattaa actgattccg ttttttgtga cgatgatttt attgccaatg
     1501 caatttttag tgtttaaccg catctttaaa aaaccccgcc cggaagaaga aaaaatagaa
     1561 aaaatgtaac actgtgaaaa ttcacactgt gaaaattcac agtgtttttt ttattggtgt
     1621 tttattaaat aatagttctt cttaccacgg cggataaccg taaactggcg ttcaatagct
     1681 tgggctttcc taacaatatg ttctaaagaa ctgattattt caccattaat actaatagct
     1741 ttgttttgga ttaattcccg agcttcacgc ttggaggaag cagctttcac caaaactaag
     1801 gtttcaatta agccgagatc ttcactaatg gcaagggaag ggacatcact aaaaccgacg
     1861 ctgatttctt tggccgttaa tgagccaatt tgcccgctga ataaggcttc cgacatggcg
     1921 atggcttggt tataggcttc ttcgccatga actaagcaca caatttcttt cgctaagact
     1981 ttttgtgctg ctcgcaaatg cggttccctt gtcacttttt cttcaagatc taaaatttgt
     2041 tcatgagaaa gaaaagtata gtatttcaaa aagcgaatga catcggcatc agccgtgtta
     2101 ataaaaaatt gatacatttc ataaggtgag gttttgtctt catccagcca aatagcgcca
     2161 ctttccgttt tcccaaactt agtgccatcg gatttagtaa tgagtggtag tgttaagcca
     2221 aaaaccttgg cttcactgcc gacaactttg cggattaaat ccattccggc ggtaatgttt
     2281 ccccattggt cctgcccgcc aatttgcatc tcgcaatgaa gattagcatc ttgatacata
     2341 tgtaaaaaat cgatgctttg gagaatcata tacgaaaact caaggtaagt taagccactg
     2401 tccaagcggg atttaacgct ttctttagcc aacatctggt taatattgaa atgctttcca
     2461 aactcccgac acatgtcgat ggcggacagt ttacttaacc attcatagtt atcaacacaa
     2521 aaagcgcgtt cgccatcaaa aactaaaaag cgcgcaaatt gttttttaaa caatgctccc
     2581 catttgtgaa cggttgccac ttcattcatt tgtcgttcgt tgcgtttccc tgacggatca
     2641 ccaattaaac cggtgccccc accggttaaa gcaatcaccc ggtgtccggc ccgataaaaa
     2701 cgcatcaagg tcacaatcgg taataatgac ccgacatgca gactatccgc cgtcggatca
     2761 aaaccgcaat ataaagtaat cggggcttct tccaagcgtt tgcttaattc ttcttcattg
     2821 ataatgtcgt agacaagtcc ccgccatttt aaatcttcca ataaattcat acttcctcct
     2881 ttaaaaataa aagtccttaa gaaaaatcct aaggacgaaa taaatccgcg gtaccacctt
     2941 agtttttaga ttgctctaaa ccctcttgtt ccctatcagc tcccaagttg taattccctc
     3001 catccggctt tggattttca ccgcccatcc aaatctctcg tgctgcccgc aatcttagta
     3061 ctgtttcttg ttcaacgctt tatttagttg attcacgcca aataacttta tagtcaacca
     3121 aaatatgttt tggtttttct tgcgcgtttg tttctctcat taattcggtt aaataagc
//
LOCUS       DAOSGN010000058         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_29359_length_3123_cov_3.778031, whole genome shotgun sequence.
ACCESSION   DAOSGN010000058 DAOSGN010000000
VERSION     DAOSGN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29359_length_3123_cov_3.778031"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="PK087_01385"
     CDS             complement(<1..1644)
                     /locus_tag="PK087_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17954.1"
                     /translation="MTTSWKFKLKFAFRNATFKKGRFVVIVFTLIMMVMLSVMMFALK
                     NYLIDIFTLEYREQYQDVDLVLTYDNYSTSRIVNVRTIKQKYAEYFDFYATFFNLHSL
                     VKVGEQTQYTQIMSSSLGEFEQVIKCDVDALNPGELFITASLAAKLKLNENASLFLEI
                     NNQDWPYIVKKIIPDQGLFQKDAIFVNKEELFEKTVWFSSPNLGNIIYLNVKPQYSLT
                     MVKEELLADEKYQNCLIYEAVDHEQVLIRAEFNFSIMITMIVVVFLAMAMIIKSLFPL
                     LFRDFSQQLGVIKLLGGSNRLALQIWVSQFVIYLSICIPLGLGLANLFLNIGARAYHI
                     YSYIGIKPLPILFSLLLFLGFMAGQIFYQYHRLTKQNELTLTRDRKAERQSSSWLYLV
                     CFGGLLVVNLLINPFPKVQALLTLLLVIGFTFMFISSSLRLLSRLWQKKKTFFTLYSV
                     KHLSQNRITHNSLKVIFISLVVLAVSLTMISMFKTTKRLAFDEIKMDYLMTNIYDYGN
                     NLKNDINANYDIDELSEGAVYHEITMNFGNNESRIIKYVL"
     gene            complement(1622..2299)
                     /locus_tag="PK087_01390"
     CDS             complement(1622..2299)
                     /locus_tag="PK087_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH17955.1"
                     /translation="MKIVSCRNLVKIYQNSKVTTPVLSGINLSIEQGEFCGITGPSGS
                     GKTTLLYTLSGLEPITDGELLLFEKNNKDYSAGEIADLRKFQIGFVFQFYNLIPNLTA
                     FENIKLAQIIAKRDDDEQVLTALEMVEMAQFKDYFPNELSGGMQQRVAIARCLVNQPQ
                     VIFADEPTGNLDQKTGQEIMTIFQKLNRQGITIILVTHSEDNLRYCSRHIKLTDGKII
                     HDHKLEV"
     gene            complement(2411..2947)
                     /locus_tag="PK087_01395"
     CDS             complement(2411..2947)
                     /locus_tag="PK087_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOH17956.1"
                     /translation="MELKGSKTEKNLWEAFSGESQARNKYTYYASQAKKEGFEQIANF
                     FTETANNEKEHAKIWFKLLHDGIPSTAENLKDAAAGEHYEWTEMYAEFAKVAKEEGFT
                     KIAYLFEAVAKIEKEHEERYLALLNNVEKESVFVKEEATVWICLNCGHLHYGKKAPNL
                     CPVCDHPQAYFEMVKKNY"
BASE COUNT         1010 a          609 c          499 g         1005 t
ORIGIN      
        1 taaaacatat ttgattatcc gtgattcatt gtttccaaaa ttcatggtta tttcgtgata
       61 tactgccccc tcactaagct catcgatatc ataattagca ttgatatcat tttttaaatt
      121 atttccataa tcatagatat tggtcatcaa ataatccatt ttaatttcat cgaaagctaa
      181 gcgcttggtg gtcttaaaca tggatatcat cgttaaggaa accgctaaaa ccactaatga
      241 aataaaaatt acttttaagg aattatgggt gatgcggttt tgtgataagt gtttgacgga
      301 ataaagagtg aaaaatgttt ttttcttttg ccataatcgc gaaagcaaac gcaaggatga
      361 agaaataaac atgaaggtaa aaccgataac caataataat gtcaataaag cttgaacttt
      421 gggaaacggg ttaattaata aattaaccac taacaagcca ccaaaacaaa caagataaag
      481 ccaggaagaa ctttgtcgtt ctgcttttcg gtcccttgtt aatgtcagtt cgttttgttt
      541 tgttaagcga tggtattgat aaaaaatctg cccagccata aaccctaaaa acaacaacaa
      601 cgaaaacaaa atgggtaagg gtttaattcc aatataggaa taaatatgat aagctctagc
      661 gccaatattc aaaaacaaat tagctaaacc aagtcctaag ggaatgcaga ttgacaaata
      721 tatgacaaac tggctaaccc agatttgcaa ggccaagcga ttgctaccgc ccagcaattt
      781 aataaccccc agttgttggg aaaaatcacg aaacagaagc ggaaacaaag atttgataat
      841 catggccatc gctaagaaaa ccaccacaat catggtaatc ataatggaga aattaaattc
      901 tgcccgaatt aaaacttgtt cgtggtcaac agcttcataa attaaacagt tttggtattt
      961 ctcatcggcc aacaactctt ctttaaccat agttaaggaa tattgcggct tgacatttaa
     1021 atagattata ttccctaaat tgggggatga gaaccacacc gttttttcaa acaattcctc
     1081 tttattgacg aagatggcat ctttttgaaa taagccttgg tcgggaataa tctttttaac
     1141 gatgtatggc cagtcctgat tattaatttc taaaaaaagg ctggcatttt cattaagctt
     1201 taatttagct gcaagcgatg cggttatgaa caattccccc ggatttaaag catcgacatc
     1261 gcatttgatt acctgctcaa attctcccaa actggaagac atgatttggg tatattgggt
     1321 ttgttctccc actttcacta aggaatgaag attaaagaag gtggcataaa aatcaaagta
     1381 ttccgcatat ttttgtttaa tggtgcgaac attaacaatc cttgaagttg aatagttatc
     1441 ataggtcagc accaaatcaa catcttgata ttgttcacga tattccaaag taaaaatatc
     1501 aattaaataa tttttcagcg caaacatcat gaccgaaagc ataaccatca tgattaaggt
     1561 gaaaacaatg accacaaacc gccctttttt aaaggtcgca ttccgaaaag caaattttaa
     1621 tttaaacttc caacttgtgg tcatgaataa tcttaccatc agttaattta atatgacgcg
     1681 aacaatagcg taaattatct tcactatggg tgacgaggat aattgttatc ccttgacggt
     1741 taagtttttg aaaaatggtc atgatttctt ggccggtttt ctggtcaaga tttcccgttg
     1801 gttcatccgc aaaaataact tgcggttgat tgaccaagca gcgagcaatc gccacgcgtt
     1861 gctgcatgcc tcccgaaagc tcattcggaa aataatcttt aaactgcgcc atttccacca
     1921 tttctaaagc cgtcagcact tgttcatcat catctctttt cgcaataatc tgcgctaatt
     1981 taatattttc aaaggctgtt aaattgggaa ttagattata aaactgaaaa acaaacccaa
     2041 tttgaaattt acgaagatcg gcaatttcac cagccgaata atccttatta tttttttcaa
     2101 ataataataa ctccccatcg gtgattggct caagtccgct taaagtataa agaagagtgg
     2161 tttttccact tccggaagga ccggtaatcc cacaaaattc cccctgttca atacttaagt
     2221 taatacccga taagacaggg gttgtcactt tcgaattttg ataaattttc actaaattcc
     2281 gacaggaaac tattttcatt tttcttcaac tctttgatta atcttaccac atttgtgcta
     2341 aatattcaat cattataatt ttatgccttt taaaaaatct caactttcaa gttgagattt
     2401 cacaatataa ctaatagttt ttcttaacca tttcaaaata agcttgggga tgatcacaaa
     2461 ccggacaaag attaggagct tttttaccat aatgtaagtg tccacagttt aagcaaatcc
     2521 aaaccgtcgc ttcttctttt acaaaaacac tttccttttc cacattgttt aaaagtgcca
     2581 aataacgttc ttcgtgttct ttttcaattt tcgccactgc ttcaaataag taagcgattt
     2641 tagtaaagcc ttcttctttg gcgactttcg caaattccgc atacatttcc gtccattcat
     2701 aatgttcacc cgccgcagca tcttttaaat tctcagcggt tgatggaatg ccatcatgca
     2761 acaatttaaa ccaaattttc gcatgttctt tttcgttgtt agccgtttcc gtaaaaaaat
     2821 tagcaatttg ttcaaagcct tcttttttag cttgcgatgc ataataggta tacttattgc
     2881 gggcttgtga ttcaccgcta aaagcttccc ataaattttt ttctgttttt gatcctttta
     2941 attccataat atcctcctct tattttttat aaaaatggtc tttggtggcc ccacataccg
     3001 ggcatctaaa ggtatcgtct tctaattcaa tatcgcggtc atgttgataa ccacagatat
     3061 cacaaacata ttttcccgtc gttttttctt cttggaaggt cggcgcatat tgcgaggtct
     3121 tcc
//
LOCUS       DAOSGN010000059         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_29746_length_3095_cov_2.866118, whole genome shotgun sequence.
ACCESSION   DAOSGN010000059 DAOSGN010000000
VERSION     DAOSGN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29746_length_3095_cov_2.866118"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PK087_01400"
     CDS             complement(<1..914)
                     /locus_tag="PK087_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17957.1"
                     /translation="MMENKDMTSYRPRPWYQNLWVNAKTGMENYFKNFRFPWKKLIIV
                     LLIGLIVLTVYLAVIFSQVKVPVRSPYDKSNFVEAPSYITAHGAQTVLENNHYRFTLN
                     NHTTTFSITDKLTLETWSSNPNTTSKRFLEPLIVYCAGALGAASPMGALEKAVEFKDY
                     YFRKQGNTLEILYEIGGKKDVDRSDFPEIMTDERMQEKILSQLEEGSIAYRRVTEQMY
                     TPGELKGKPVWKLKDGITVVPLRNLYKIFYEDCGYTTEDLQYDLELSGIVYEDLYPYV
                     EIAIRYALSDEGFVVEIINESIVEKAKYP"
     gene            complement(911..1600)
                     /locus_tag="PK087_01405"
     CDS             complement(911..1600)
                     /locus_tag="PK087_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOH17958.1"
                     /translation="MNSEIKKVRSFPRFNLKQALKSFFREAFVHPFYLLTHPIKGFDA
                     FKREKTSRGYVAIFYLVMMIITQILAYNKNGFLINRRNPDDFNLFLTIALVIFPVIIA
                     TVANWSSTVLMDGKGTMKEIFRVICYSFFPYVWLGLLATLISNFITIDEIIFFNFLTG
                     LGAFLLVYLLFFGLMGIHEYGLLKTILMILATILAIAVILFIMLLFLSLIQQVYAYIS
                     SVYREFVMRFL"
     gene            complement(1593..3071)
                     /locus_tag="PK087_01410"
     CDS             complement(1593..3071)
                     /locus_tag="PK087_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17959.1"
                     /translation="MKKIISLLLSLVLLLSVSVVSVQGAYNYSPLGEVIASAEALIAT
                     KVVDSSNLRTTNDEKAALVFGVLSDICSFNNKIYLVDGSNNLIHVLNNDFQYERSLGQ
                     GQLKQPQGLFVTDEYLYVADTGHFRIALFDHDGLFVRAITAPDDPTFKQGPDDTSGYD
                     FKPIKVAVDRTGRVYAIADQIFEGIIDFNPDGSFSRYVGANTVTLSVWDAFWLQFTTE
                     EQRKSQGYRFATTFLNLNIDEKGYLYTLSSVTEGTRVIKKLNYKGRDVLTRNGYVPLV
                     GDASYLTYQDKVPLGPSQLIDIDINEDGFYTVLDGVRGRMFTYDFEGNLLYIGGQIGN
                     VGGSTNNQSTLFISPKALCFHNEQILVIDALNKNLVVFQYTEFGRLVNEATHLYQLGK
                     YAEAQSVWEQVLLLNTNYYLAYAGIGKAQLRAGDYQQALVNLKLGYDTYNYSQAYQQY
                     RYDQLTKVFPFIMGVVIVVLVYAFVKSIKKSVLNEVKEEKDE"
BASE COUNT          974 a          649 c          587 g          885 t
ORIGIN      
        1 gggtatttgg ctttttcaac aatcgattcg ttgatgattt ccacaacaaa tccctcatcg
       61 ctaagcgcat agcgaatcgc tatttccaca tagggataga gatcttcata aacaatcccg
      121 cttaactcta aatcgtattg gaggtcctct gtggtatagc cacaatcttc atagaagatt
      181 ttatagagat tacgcaaggg aacaaccgta attccgtcct tcaatttcca aacgggcttg
      241 ccttttagtt ctcccggtgt gtacatttgt tccgtaaccc ggcggtaagc aatactcccc
      301 tcttccagtt gcgaaagaat tttttcttgc atccgttcat cggtcatgat ttccggaaaa
      361 tcgctgcggt caacatcttt tttgccgccg atttcataaa gaatttctaa ggtattacct
      421 tgttttcgaa aataataatc tttaaattcc accgcttttt ccaatgcccc catcggtgat
      481 gctgcgccta aggcaccggc acagtaaacg attaagggct ctaaaaaccg tttggaagtc
      541 gtattcgggt tgctcgacca tgtttccagt gtcagtttat ccgttattga aaaagtggtc
      601 gtgtggttgt ttaaggtaaa gcgataatga ttattctcca aaaccgtttg ggcaccgtga
      661 gctgtaatat aactaggggc ctccacaaaa ttgcttttat catagggact gcgcactggc
      721 actttgactt gactaaagat gacggctaaa tagacagtta agacaattaa tcctatcaaa
      781 aggacaatga ttagtttttt ccacggaaag cggaaatttt taaaatagtt ttccattccc
      841 gttttcgcat tcacccataa attttgatac cacggtcgtg gtcggtagct tgtcatgtct
      901 ttgttttcca tcataggaac ctcatcacaa actctcgata gaccgaggaa atataagcat
      961 aaacttgttg aatcaaagat aggaacaata acataatgaa caaaatgacc gcaatcgcta
     1021 aaatggtcgc gagaatcatc aaaatcgttt tcaataaacc atattcatga atccccatta
     1081 aaccaaaaaa caatagataa acgagcaaaa atgctcccaa tccggttaaa aaattaaaga
     1141 aaattatttc atcaatggtg atgaaattgc tgattaaagt cgccagcaag cctaaccaaa
     1201 cataggggaa gaaggaataa cagatgacgc ggaagatttc tttcattgtt cctttgccat
     1261 ccatcaagac cgttgatgac caattggcga cggtcgcaat gataaccgga aaaatgacca
     1321 aagcaatcgt taaaaacaaa ttgaaatcat cgggattgcg gcggttaatc aaaaaaccgt
     1381 ttttgttata agctaagatt tgggtgataa tcatcatcac taaataaaag atggctacat
     1441 accctcggga agttttttct cttttaaagg catcaaagcc tttaataggg tgcgtcaaca
     1501 aatagaaagg atggacaaaa gcttccctaa aaaagctttt taaagcttgt tttaaattaa
     1561 agcggggaaa agagcgaact ttttttattt cactattcat ctttttcctc ctttacttca
     1621 ttaagtacag atttcttaat cgacttcaca aaggcatata ccaaaacaac aatgacaacg
     1681 cccatgataa aaggaaaaac cttggttaat tgatcgtaac ggtattgttg gtaagcctgg
     1741 gaataattat aggtatcata accaagtttt aaattgacca gtgcttgttg gtaatcgccc
     1801 gcccgcagtt gggctttgcc gataccggca taggctaagt aataattcgt gttaagcaat
     1861 aatacctgtt cccaaacgct ttgggcttcc gcatattttc ctagttgata taaatgcgtg
     1921 gcttcattaa ctaagcggcc gaattccgta tattgaaaaa caaccaaatt tttattgagg
     1981 gcatcaatca ctaaaatttg ctcattgtga aaacagaggg ctttcgggct gataaagagc
     2041 gtactttgat tgttggtcga accgccgaca ttcccgattt gtccgccgat atataataaa
     2101 ttgccttcaa aatcataggt aaacatccgc ccccgaacac cgtccaaaac tgtataaaaa
     2161 ccgtcttcat taatatcgat atcaattaat tggctgggtc caaggggaac tttatcttga
     2221 tatgttaaat agctggcatc gccgactaag ggaacataac cgttgcgggt aaggacatca
     2281 cggcctttgt agtttaattt tttaataact cttgtgcctt cggtcacact ggataaagta
     2341 tataaataac ctttttcatc aatgtttaaa tttaaaaaag tcgtagcaaa acgataacct
     2401 tgacttttac gctgttcttc cgtagtaaat tgcaaccaaa aagcatccca aacggagaga
     2461 gtgacagtat tggcaccaac atagcgcgaa aaactgccgt cggggttaaa atcaataatg
     2521 ccttcaaaaa tttggtcggc aatggcataa acccggccgg tgcggtccac cgccacttta
     2581 atcggtttaa agtcataacc actggtgtca tcggggcctt gtttgaaggt cgggtcatcg
     2641 ggagcggtaa tcgcccgcac aaagaggccg tcatggtcaa acaaagcaat ccggaaatga
     2701 cccgtatcgg ccacatagag atactcatcc gtcacaaaca agccttgtgg ttgttttaat
     2761 tgcccttgac caaggcttct ttcatattga aagtcattgt ttaaaacatg aattaaatta
     2821 tttgaaccgt caactagata aattttgttg ttgaaagaac aaatatcact taagacacca
     2881 aagactaaag cggctttctc atcgtttgtc gttcttaaat tggaagaatc aacgactttc
     2941 gtcgcaatca aggcttctgc cgaagcaatc acttcgccaa gggggctata attataagcc
     3001 ccttgaacgg atacgacact caccgacaat aacaaaacta aactaagtaa gagactgatt
     3061 atctttttca taagtccctc ctatttcaag ccggg
//
LOCUS       DAOSGN010000060         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_30427_length_3045_cov_3.230435, whole genome shotgun sequence.
ACCESSION   DAOSGN010000060 DAOSGN010000000
VERSION     DAOSGN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30427_length_3045_cov_3.230435"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1036
                     /locus_tag="PK087_01415"
     CDS             224..1036
                     /locus_tag="PK087_01415"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOH17960.1"
                     /translation="MNWFEKIIDFLSYQLPSSPLPYGRFHLLFLGLTFISCFLIALKL
                     RHSNDKQDRFILLTLSVLMLSFEVYKQLVFTIEKDVWDYQWYVFPFQFCSVPMYVAFI
                     TAFLKPGKMKNACYNFLGTFCLFAGLAAMFYPKDVFIRILGIDIQTMVHHSSMILIGF
                     YCLISGRTVLQQKSIIGSPLIFFVLFIMALLMNLLGKNIGEVFNMFFISPYYACHLPV
                     LSQIQNQFGYYVFLLAYLFGFILLAYLILLTAIAIKKWHKQTKKLPKSFKAN"
     gene            complement(1061..2524)
                     /locus_tag="PK087_01420"
     CDS             complement(1061..2524)
                     /locus_tag="PK087_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOH17961.1"
                     /translation="MKTNIGLIPLDSRPCNYDWPLTLSKLANVNLITLDKSCLGTLHT
                     KLDFAHIQSFLDEKLDCLHFLICPLDSIISGGLIQSRKANFSHDELNYKINYLKTLKK
                     RNPKLKIYVFDTILRTSISTFDEKTKQYWQLINEYSRYKGQYLRFHKQEDYDEFQKIS
                     LQIPVEVLNTYEVARLKKHEVNQLALDLLQQKAIDFLLLLQEDSMPKGLQTLEHDVLN
                     KIIQHKHLENKAWLYNGADEGSCFLLAKIISDIHEQPLKYYLLHPDEALLALPQAFED
                     RPYLENLSKMTKILQMQRTDDLKEAAFVLAIYQNNPLPKMEIADREQALKMVNDDKTR
                     AFINHINMLLKQTIPVFFLDINVPNGGCWELLKQIDFLKLKAYSAWNTASNSTGSLLA
                     TAAIYCLQAKPDPSLTNLFLQERIIDDCFYQDVVRGKIHEYLRTNLLNLFDFKPHHEV
                     KQLLQRELHALSDPFFKTVYRAYFPWNRAFEIGISYD"
     gene            2710..>3045
                     /locus_tag="PK087_01425"
     CDS             2710..>3045
                     /locus_tag="PK087_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17962.1"
                     /translation="MLKKLIIACLMLFSVFMFVGCKKDDEVVESLNKIKIYGPFDTFD
                     PEKDYVHGLIKEITGVDVDYDFLPQDGASANTKLYLDIAGQAEYHLLKLDPTQFDQLR
                     AVGALLDITE"
BASE COUNT          914 a          480 c          580 g         1071 t
ORIGIN      
        1 gatttaaaga aagctccggc gtttggtttg tgaaaagatt ggaaatactt ggagttaagg
       61 agttgttatc tttaaccgct tatgttcggg gtggttgcgg tcccatgggg atgccttatc
      121 aatatcctac ttacattgat gaataggcca ccactttaca aaaagccttg tcgcttaaac
      181 tggctaagtt aacgacagcc ctctagaaag tgattatttt gctatgaatt ggtttgaaaa
      241 aatcattgat tttttaagtt atcaattgcc aagttctccc cttccctacg ggagatttca
      301 tttattattt ttaggattga cttttataag ttgttttttg attgctttga aacttcgaca
      361 cagcaatgat aaacaagatc gatttatctt attgacttta agtgttttga tgttgagttt
      421 tgaagtttat aagcaattgg tttttacaat cgaaaaagat gtttgggatt atcaatggta
      481 tgtttttccg tttcagtttt gttccgtacc gatgtatgtt gctttcatta ccgctttttt
      541 gaaacccgga aaaatgaaaa acgcttgtta taatttttta ggtacttttt gcttgtttgc
      601 cggtttggcg gcaatgtttt accccaagga tgtttttatt cgcattcttg gtattgacat
      661 ccaaacgatg gttcaccata gttcgatgat tttaattggc ttttattgtt taatttccgg
      721 gcggacggtt ttacaacaaa aaagcattat cggaagtcca ttaatcttct ttgtgctgtt
      781 tatcatggct cttttgatga atctattggg taagaatatc ggcgaagtat ttaatatgtt
      841 ttttattagt ccttattacg catgtcactt accggtgtta tcgcaaattc agaaccaatt
      901 tggatattat gttttcttgc ttgcatattt atttggtttt attttacttg cgtacctgat
      961 tcttttgaca gctatcgcta ttaaaaagtg gcataagcaa acaaagaaat taccaaaatc
     1021 ctttaaggca aactaaaaaa gcaccgatgt gcttttttta ttagtcataa gatatgccga
     1081 tttcaaaagc gcggttccaa ggaaaatagg cacggtaaac ggttttaaaa aaggggtcgg
     1141 acaaagcatg taattctcgt tgtaataatt gcttgacttc atgatgcggt ttaaagtcaa
     1201 acaggtttaa taagttggtt cgcagatatt catgaatttt ccctctaacg acatcttggt
     1261 agaaacaatc atcaataatt cgttcttgta agaaaagatt agttaatgaa ggatcgggct
     1321 ttgcttgtaa acaataaata gcggctgttg ctagtaaact acccgttgaa ttggaagcgg
     1381 tattccaagc gctataagct ttaagcttaa gaaaatcaat ttgtttcaat aattcccaac
     1441 aaccaccgtt aggaacatta atatccaaga aaaagaccgg aatggtttgt tttaaaagca
     1501 tattgatgtg attgataaaa gcccttgttt tatcgtcatt aaccattttc agtgcttgtt
     1561 cacggtcggc tatttccatt tttggtaaag gattattttg gtaaatcgct aaaacaaagg
     1621 ccgcttcctt aaggtcatcc gttctttgca tctgcagtat tttagtcatt ttacttaagt
     1681 tttctaaata agggcgatct tcaaatgctt ggggaagggc tagtaaagct tcatccggat
     1741 gaagtaaata atactttaat ggttgttcgt ggatatcgct gataatttta gctaataaaa
     1801 aacaactgcc ttcatccgcg ccgttatata accaggcttt gttttctaaa tgtttatgtt
     1861 gaatgatttt gtttaacaca tcatgttcca atgtttgcag gcccttgggc atcgagtctt
     1921 cctgaagcag caacaagaaa tcgatggctt tttgctgtaa taaatctaag gctaattgat
     1981 tgacctcatg tttttttaaa cgggcaactt cgtaagtatt aagaacttca acgggaattt
     2041 gcaaactgat tttttgaaat tcatcataat cttcttgttt gtggaagcgc aaatattgac
     2101 ctttatagcg actgtattcg ttgattaatt gccaatattg tttggttttc tcgtcgaatg
     2161 tcgaaatgga agtccgcaag atggtatcaa aaacatatat ttttagtttt ggattgcgtt
     2221 ttttgagggt ttttaaataa tttattttgt agtttaattc atcgtgcgaa aaattggctt
     2281 tgcgactttg aattaagccc ccgctgataa tactgtcaag cggacaaatt aaaaaatgca
     2341 aacaatcgag tttctcatct aaaaaacttt ggatatgggc gaaatcaagt ttagtatgca
     2401 atgttcctaa acatgattta tcaagagtga ttaaattgac attggccaac tttgataaag
     2461 taagaggcca atcgtaatta caaggacgac tatctagggg tattaagcca atatttgttt
     2521 tcataaaagc tccttttttt gcttttattg taacacagcc aaaataaaaa gagaatgata
     2581 aaataatcaa taatcagttg actagacatt ttgttggtga tataatgata gttgtgatga
     2641 aagcgttatt ttataacgat tatcgtctac tatgacagcc tattaagctg aaaagaaaag
     2701 gagaaaaaca tgctaaaaaa actgatcatt gcctgtttaa tgttgttttc ggttttcatg
     2761 tttgtcggat gtaaaaaaga cgacgaagtt gttgaaagcc taaacaaaat taaaatttat
     2821 ggcccgttcg acacatttga cccggaaaag gattatgttc atggcttaat caaagaaatt
     2881 accggtgtag atgtcgatta tgattttttg ccacaggatg gtgcctccgc aaacactaaa
     2941 ctttatttag acattgccgg acaagctgaa taccatctat taaaacttga tcctactcaa
     3001 tttgaccaat tacgtgcagt tggcgctttg ttggacatta cggaa
//
LOCUS       DAOSGN010000061         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_30678_length_3026_cov_3.365533, whole genome shotgun sequence.
ACCESSION   DAOSGN010000061 DAOSGN010000000
VERSION     DAOSGN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30678_length_3026_cov_3.365533"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PK087_01430"
     CDS             complement(<1..1322)
                     /locus_tag="PK087_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH17963.1"
                     /translation="MQTQDYSQDQDILKKFGRDIVEEVKKGKVDPVIGRDEEIRRIIQ
                     VLARKTKNNVILIGEAGVGKTAIIEGLASRIAKDDVPASLRGKTIYELDMGALVAGAK
                     FRGEFEERLKAVLNKIKESEGKIILFIDEIHLIVGAGKADGALDAGNMLKPMLARGEI
                     DCIGATTLNEYRLYIEKDRALERRFQQVLVTEPTVADTISILRGLKERFEAHHGVTIT
                     DGALVNAAVLSNRYLTERLLPDKAIDLIDEACASIRVEMDSMPNELDEITRKIAQLEI
                     EKLALEKEKDASSKQRLAKIVDELNELRERSAEMTSRWQAEKNKIEEAKEVKKKLEKA
                     RFDLEQYFNTGDYKKASELKYGEIPALEAKLEKLQKKGENSLLTETVTEDAVAKVVVK
                     ATNIPLSKIMKGDREKVLNLKETLNERVIGQDEAIELVSDAIIRQRAG"
     gene            complement(1463..2461)
                     /locus_tag="PK087_01435"
     CDS             complement(1463..2461)
                     /locus_tag="PK087_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17964.1"
                     /translation="MNDLVARYIYDVTRRLPEENRAEVKKELQANISDMLPSNPTEEQ
                     IKEVLKTLGNPRVLAHNYLGKSKSLISAEWMDDYLRVLKIVLIIFGTLAFIFGLIEHI
                     SHPEATKLIGIILEVIAKVISETIQSLIRGFAIVTIVFALISRYASPEKIKAWEPSQL
                     PKLPKEQVVTIKRTSSVAGLIFTVIFRVLLVFLLINRRLYLAWTADGEWWVRDTLMFN
                     DAAVKLFVPFIIISIVFVIVTYVTKIYFGHWNKVVAGIHTGEQLYTNVIALLFINHKP
                     LLTAEFVKGAADIFEVSVTKMQEIFSGIAIVISVMIVLGVIGDLVTTWIKTLKVKA"
     gene            complement(2458..2802)
                     /locus_tag="PK087_01440"
     CDS             complement(2458..2802)
                     /locus_tag="PK087_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOH17965.1"
                     /translation="MRLDESIVQNMILELRRGTQILAVLSQMDEMKYGYSLLQDLESQ
                     NIKIEAGTLYPLLRRLESQGLLLSEWDTTESRPRKYYLLSDFGKQALETLIKEWDLIV
                     KEMSNIIKGGQK"
BASE COUNT          829 a          729 c          508 g          960 t
ORIGIN      
        1 atgccggcgc gttggcgaat aatggcatcg ctgactaatt caattgcttc atcctgaccg
       61 atgacgcgtt cgtttaaagt ctcttttaaa ttgagcactt tttcccgatc gcctttcatg
      121 attttggaaa gcgggatgtt cgttgctttc acaaccactt tcgctaccgc atcttcagtc
      181 accgtttccg ttagtaaact gttttcccct tttttctgca gtttttccag cttggcttcc
      241 aaagcgggaa tttccccata cttcagttca ctcgcttttt tataatcacc cgtattaaaa
      301 tactgttcta aatcaaaacg cgctttttcc agtttcttct tgacttcctt ggcttcttcg
      361 attttattct tttccgcctg ccaacgcgaa gtcatttcgg cggaacgttc ccttaattca
      421 tttaattcat ccacaatctt cgctaatctt tgtttactgg aagcatcttt ttctttttca
      481 agggccagtt tttcaatttc caattgcgca attttccgag taatctcatc cagttcgttg
      541 ggcatggaat ccatttccac ccgaatggaa gcacaggctt catcaattaa atcaatcgct
      601 ttatcgggaa gtaacctttc ggttaaataa cgattggata agacggccgc attgactaaa
      661 gcaccgtcgg taatcgttac cccatgatgg gcttcaaagc gttctttcag accccgtaaa
      721 atactgatag tatcggccac ggtcggttcc gtaaccaaga cctgctgaaa acgccgctcc
      781 aaagcacggt ccttttcaat atataaacgg tattcattta gtgttgttgc gccgatacaa
      841 tcgatttccc cccgagccaa catcggcttt aacatattgc cggcatccaa agcgccatcg
      901 gctttaccgg cgccgacaat taaatgaatc tcgtcaataa acaaaataat cttgccttcg
      961 ctttctttga ttttgtttaa gacggctttt aaacgctctt caaattcccc ccggaattta
     1021 gctccggcca ccaatgcccc catatcgagt tcataaatcg ttttgcctcg caaactggcg
     1081 ggaacatcat ccttcgcaat ccggctggct aagccttcaa taatggcggt tttcccaacc
     1141 ccggcttcgc caattaaaat aacattgttt ttcgttttac gggccaacac ctgaatgatg
     1201 cggcgaattt cttcgtcacg accaatgacg ggatcaacct tgcctttttt gacttcctca
     1261 acaatatccc ggccaaactt ctttaatata tcttgatctt gactataatc ttgtgtttgc
     1321 ataatatccc tccatttatt tattagcact cacaaccaaa gagtgctaat tatattatac
     1381 acgctttcgc ctacttgtca agtcttctac ttaaaataat cccaaataaa acaaaaccct
     1441 ccaaaagaag ggttgttatt gtttaagctt ttacttttaa agttttaatc caggtcgtaa
     1501 cgagatcccc aatcacccca agcacaatca ttacagagat aacaatggcg atgccggaga
     1561 aaatttcttg catcttcgtt acggaaactt cgaagatatc ggcagccccc ttcacaaatt
     1621 ccgctgtcag taagggtttg tggttaataa acaaaagggc aattacattt gtatatagtt
     1681 gttcaccggt atgaattccg gccacaactt tattccaatg tccgaaatat attttcgtaa
     1741 cataagtaac aatcacaaaa acaatcgata tgataataaa gggaacaaac aatttcaccg
     1801 ccgcgtcgtt aaacattaaa gtatccctta cccaccattc gccatcagcc gtccacgcta
     1861 aataaaggcg acggttgatt aataagaaaa caagcaaaac ccggaaaata accgtgaaga
     1921 ttaagccggc aacactggaa gttcttttaa tcgtcaccac ttgttctttc gggagtttcg
     1981 gtaattggct cggttcccaa gccttaattt tttccgggct tgcataacgg gatattaaag
     2041 caaacacaat cgtcacaatc gcaaatccac gaattaaact ttggatggtt tcggaaatga
     2101 ccttagcgat gacttccaaa atgatgccga ttaatttcgt tgcttccgga tgactgatat
     2161 gttcaatcaa gccgaaaata aaggctaaag ttccaaaaat aattaagaca atttttaaga
     2221 cgcggagata atcatccatc cactctgccg aaattaaact cttactttta ccaagataat
     2281 tgtgagcgag aacacgcggg ttccctaaag tttttaatac ttccttaatt tgttcttccg
     2341 tcgggttgct tggcaacata tcactgatat tggcctgtaa ttctttcttc acctccgccc
     2401 ggttttcttc cggtaaccgg cgggtcacat cgtaaatgta tcgggctact aaatcgttca
     2461 tttttgtcct cctttaataa tgttactcat ttctttcacg attaaatccc attctttgat
     2521 taaagtttct aaagcttgtt tcccaaagtc gcttaataaa taatatttcc gcggccggct
     2581 ttccgttgtg tcccattcgc taagcaataa gccttgtgat tccaaccttc ttaacaaggg
     2641 gtaaagcgtc cccgcttcaa ttttaatgtt ttggctttct aaatcttgca agagcgaata
     2701 tccgtatttc atctcatcca tttggcttaa aaccgccaag atttgcgttc cgcgacgcaa
     2761 ttctaaaatc atgttttgta caattgattc atccaatctt atcacctcga cactattata
     2821 ctatatgtca tacaatattg tcaagtaaaa aatattaaat ttttttagtt attaaaaaac
     2881 tccctgttat tcagggagtt ttgcttgttt gttgatgatt gcccgggcaa tacaaacacc
     2941 gttagctccg gcttgagcca agcctctagt aatcccggct ccgtcgccac caacatataa
     3001 atttttaatt ttggtttcaa agtgat
//
LOCUS       DAOSGN010000062         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_30766_length_3020_cov_4.044182, whole genome shotgun sequence.
ACCESSION   DAOSGN010000062 DAOSGN010000000
VERSION     DAOSGN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30766_length_3020_cov_4.044182"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PK087_01445"
     CDS             <1..883
                     /locus_tag="PK087_01445"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH17966.1"
                     /translation="EPLTMMVPSGIPLIAVGSFLTDEQMEITDVNNPSLLVSAMTSQS
                     HDIVIAPLNVGVKVHNSGNEVYQLAGILTFGNSYVVTRKTHSLTKISDLEGQKMLAYG
                     QNSTPDIVLRTALKLNGVTTSVDYQANVNLALPFFLCNTNNPNDETCSEYPYLLAAEP
                     NISMLEVKYDLELNVLDLQEVLKTRIDKIPQAGVFVRLDPSKKTQINRFLDTLTTKTT
                     AMNANPKQYAESIFNNHSFFESLTLPVLEKCIPRSQIKFEKVTAQTQYMLDYYELVMA
                     LDPLLLGNKLPKEGFYR"
     gene            880..1614
                     /locus_tag="PK087_01450"
     CDS             880..1614
                     /locus_tag="PK087_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH17967.1"
                     /translation="MKKPFKGYYVSGLLFLFLIWIISSAIIKNDLAVPRFLVVVQALK
                     IMIIDSATWLAIGNTCFSLLVTVLICFGVALLLSILVVYFKGLELFLSPLLAVLRTIP
                     VIVVMVVLLMVFGNRQSPMIMTGFVLLPLLYEQYVTAFKTTNLEITDDLSLLGATKWQ
                     AFRFVYFPLSFPVVLSSLVQSFGLGLKVMVMAEYLASPKQTIGYALMQAVTTLNTALV
                     FAWTVILIIFVLLVETLIKKIRIEKW"
     gene            1685..2305
                     /locus_tag="PK087_01455"
     CDS             1685..2305
                     /locus_tag="PK087_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH17968.1"
                     /translation="MGIMTFKNLKKDKRERILEVALANFSTQAWEKITISQIVGQSQI
                     PRGSFYQYFDTIHDLFAILLVYLFRKEQEPFGKWLGESNGDLKQALQLRFIRIMKHLE
                     VYEQGWFLKNLLYFKSVNGELSLPAPINPQFDDETKAVINRVLRRESSDLLSYLEKLF
                     TSCFLSYLEDSTSKEMLIRYFNQSLDLLFAKEEHTRRKNEVIIKDI"
     gene            2280..>3020
                     /locus_tag="PK087_01460"
     CDS             2280..>3020
                     /locus_tag="PK087_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOH17969.1"
                     /translation="MKSLLKIYKRSYSKKYLAYAILAILFMIGDVGIALAIPAITKGI
                     FTEINSGRNSLSYVYQTGLLVVAVALVAVITTILNNVFAQHLSTKITRDLRKDLFAKV
                     QDLSFSNVDKITSGTLMTVIANDTSQIQQIIMMSFRAILRSPLTLIGALVMAFVTNKD
                     LLYIILGVVVILGFSVMKIITKAAKIFSSLQEKNDDLNEKLIETISGAREIKVFQTET
                     ADFEKFTKTNEEYTNTIVKANKHMALMDP"
BASE COUNT          913 a          515 c          599 g          993 t
ORIGIN      
        1 agaaccacta accatgatgg ttccgtccgg aattcctttg attgcggttg gttcgttttt
       61 aaccgatgaa caaatggaaa taactgatgt gaacaatcct agtttgctcg tgtccgctat
      121 gacttctcaa agtcatgaca ttgtcattgc gcccttaaat gtcggcgtta aggttcataa
      181 cagcggtaat gaggtttatc aattagccgg tattttaact ttcgggaatt cctatgtggt
      241 cacgcgaaaa acacattcct taacgaaaat aagtgattta gaaggccaaa aaatgttggc
      301 ttatggccaa aacagtacgc ctgacattgt tttgcggacg gctttaaaac taaatggagt
      361 cacaacttcc gttgactatc aggcgaatgt gaatttagca cttcctttct ttttgtgtaa
      421 cactaataat cctaatgacg aaacatgcag tgagtaccct tatttgttag cggctgaacc
      481 caatatttcc atgctggaag taaaatatga tttggaatta aatgtgcttg atttgcaaga
      541 agtattaaaa acccgaatcg ataaaatccc ccaagccgga gtctttgttc ggcttgatcc
      601 ttccaagaaa acccaaatca atcgattttt agacacatta acaacgaaga cgaccgctat
      661 gaatgctaac ccgaagcaat atgcggaatc gatttttaat aatcattcat tttttgaaag
      721 tctaactttg ccggttttgg aaaagtgcat tcctagaagc caaattaagt ttgaaaaagt
      781 cacggctcaa acccaatata tgttagatta ttacgaatta gtgatggcgc ttgatccgct
      841 tcttttagga aacaagttgc ctaaagaagg attttatcga tgaaaaaacc ctttaagggt
      901 tattatgtca gcggattatt gtttttgttt ttaatttgga ttatcagtag cgcaatcatt
      961 aaaaatgatt tagctgtgcc tcggtttttg gtggtggtgc aggctttaaa aatcatgatt
     1021 attgattccg ccacttggtt ggcaattgga aatacttgct ttagtttgct ggtaacggtt
     1081 ttaatctgct ttggtgttgc gcttttgtta agcatcttgg tagtttattt caaaggcttg
     1141 gaattgtttt taagtccgct tttagcggtt ctccgaacaa ttccggttat tgtggtgatg
     1201 gttgttttac taatggtatt tggtaatcgg caaagcccaa tgattatgac gggatttgtg
     1261 ttgttaccgc tattgtatga acaatatgtt acggccttta aaacaacgaa tctggaaatt
     1321 actgatgacc ttagtttgct tggagccacg aaatggcaag ccttccgctt cgtttatttt
     1381 cccctttcgt ttccggttgt tttatccagt cttgttcaaa gttttggttt aggtttaaaa
     1441 gtgatggtga tggcggaata tcttgcttca ccaaaacaaa caatcggtta tgccttgatg
     1501 caagctgtta cgactttaaa tacggccttg gtttttgctt ggacagtgat actaattatt
     1561 ttcgttttat tagtggaaac tttaatcaaa aaaatcagga ttgaaaaatg gtaaattttt
     1621 catccttttt tttgacactt ttttacttat atgataaaat aactatcact tatggagcta
     1681 acaaatgggc ataatgacat ttaaaaacct aaaaaaagat aagagggaac gcattttgga
     1741 ggtggcgttg gccaattttt ctacccaagc ttgggaaaaa attaccatct ctcaaattgt
     1801 tggacagtcc caaattccta ggggcagctt ttaccaatat tttgatacca tccatgattt
     1861 atttgctatc ttgttggtat atttatttag gaaggaacaa gagccgtttg gaaaatggct
     1921 tggagaaagt aatggggatc tcaagcaagc gctccaattg cgatttatta gaattatgaa
     1981 acacttggaa gtttacgaac aaggatggtt tttaaaaaat ttactttatt ttaaatcagt
     2041 taatggcgaa ctaagccttc cggccccaat taatcctcag ttcgacgatg aaactaaagc
     2101 tgtgattaat cgggttttgc ggagggaaag ttcggattta ctttcctatt tggaaaaact
     2161 ttttacatcc tgttttttgt cctatttaga agactcaaca agcaaagaaa tgctgattag
     2221 atattttaac caaagtttag atttattgtt tgcgaaagaa gaacacacga ggagaaagaa
     2281 tgaagtcatt attaaagata tataaacgca gttactcgaa aaaatatctt gcttatgcca
     2341 tcttggcaat tttattcatg attggcgatg tcggcattgc tttagctatt cctgccatta
     2401 ccaaaggtat ttttacggaa atcaatagcg gccgaaacag tttatcctat gtctaccaaa
     2461 ccggcttatt agtagtagcg gtggctttgg tggcggttat aaccaccatt ttaaacaatg
     2521 tttttgcgca acatttatcc acgaaaataa ctagggattt gcggaaagat ttgtttgcta
     2581 aagttcagga tttatcgttt tccaatgtgg acaaaatcac ctccggaact ttaatgaccg
     2641 tgattgcgaa cgatacaagc cagattcaac aaatcatcat gatgagtttt cgggcgattt
     2701 tacgaagccc tttaaccttg attggagcct tagtgatggc ttttgttacc aacaaggatc
     2761 ttttgtatat tattttagga gtcgtcgtta ttttaggttt ttccgttatg aagattatta
     2821 cgaaagccgc taaaatcttt tccagtttac aagaaaagaa tgatgactta aacgaaaaac
     2881 tgattgaaac cattagtgga gccagagaaa taaaagtttt ccaaacggaa actgccgatt
     2941 ttgaaaaatt cacgaaaacc aacgaagaat atacgaatac aatcgttaaa gccaataaac
     3001 atatggcctt gatggatccg
//
LOCUS       DAOSGN010000063         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_30808_length_3018_cov_2.030712, whole genome shotgun sequence.
ACCESSION   DAOSGN010000063 DAOSGN010000000
VERSION     DAOSGN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30808_length_3018_cov_2.030712"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /gene="argS"
                     /locus_tag="PK087_01465"
     CDS             <1..1223
                     /gene="argS"
                     /locus_tag="PK087_01465"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOH17970.1"
                     /translation="SYGDSLARILRKAGYSVSTEDYVNDAGNQINNLIFSINERYREL
                     FGLPCQLNEDSYHGKEIIELAKQLQSLYGDYYLHHPWEKTFRQFGVDYLLAGLKKDLA
                     DFNVTFDTWFSEQALYDNGEVEKVKNYLISQGHTYELDGAIWLKTTQYEDEKDRVLVK
                     SDQTYTYLLPDIAYHANKLKRGFTHLIDVLGADHHGYINRLKAAIAFVGGNPDQVDIK
                     ILQMVRVLQNGEEIKMSKRSGKAITLRDLMDEVGTDALRFMYVSKALNTHMDLDLDLA
                     VQNSNDNPVYYVQYAYARIASVFRVVEEKGFLFQAVNSFSHLHLESIEQLVLQLVQYP
                     LIIEEAATKRLPHKLAQYLLDLAQALHTYYNDERIITDNWDEVREKLTVLKAVQIVLK
                     DGLNLIGVNTKERM"
     gene            1232..1909
                     /locus_tag="PK087_01470"
     CDS             1232..1909
                     /locus_tag="PK087_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17971.1"
                     /translation="MKEFFKKYLFVFEWIGAAILIGVGIYVVLDSSILNVFVGLILLV
                     FGLLRLIPLIKTTPDKVLKIMYACEIVINVGAGIFLVIEGGKENSNLNDWFGYMIGGV
                     LMLRGFVHFFATSLRKEPNDYVKFFSHLGLFTVGCFIVARGGFSSDTLAYIILVLAIL
                     SALFIGFSGYKHYRNYRYELRAREESKKVVVQDEKQPEQIIEDPKPKKSKIITPEEEK
                     KDEAILS"
     gene            1922..2998
                     /locus_tag="PK087_01475"
     CDS             1922..2998
                     /locus_tag="PK087_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOH17972.1"
                     /translation="MNQERLFLKKLTEIDGVSGHEKAVSQFLEKELLTFVNQNDVSFD
                     GLGSIVAKKVGDANGPKIMISGHMDEIGMIVTKITDEGFIRFQTIGGWWSQVMLAQQY
                     VITTGKGKRIFALMGSKPPHLLTSDDFKKPANIDEMFLDIGVDNKEEVEKLGIEIGDM
                     IAPNIAFRELANPQYLLGKAWDNRIGCAVVVETLRRLKKENHPNIVYGVGSVQEEVGL
                     RGAKTAAQMINPDLCFALDVGIAKDTPGTDMSVKMGKGPAILVYDSALIGHVDLRNFV
                     IQVAKEENIPYQLDYLKRGGTDAGTAHLVHSGVPSMSLCIPTRYIHSHTSILHYDDYE
                     NAIRLLVAVIKKLDHQTVKDITFA"
BASE COUNT          909 a          496 c          640 g          973 t
ORIGIN      
        1 cttcttatgg tgattcatta gctcgcattc ttagaaaagc ggggtattcg gtcagcacgg
       61 aagactatgt taatgatgcg ggcaatcaaa tcaacaattt gattttttcc attaatgaac
      121 gttatcgaga gttgtttggc ttaccttgtc aacttaatga agactcttat cacgggaaag
      181 aaattattga attagctaaa caattgcagt ctttatatgg tgattattat ttgcaccacc
      241 cttgggaaaa aacttttcgc caatttggtg ttgattattt attagctggt ttaaaaaaag
      301 atttggccga ttttaatgtt acctttgata cttggttcag tgaacaagcc ctttatgaca
      361 atggagaagt ggaaaaagtt aaaaactatt tgattagtca agggcacacc tatgaacttg
      421 atggtgccat ttggctgaaa actacacagt atgaagatga aaaagaccgc gttttagtta
      481 agagtgatca aacatatacc tatttactcc cagatattgc ttatcatgcc aataaattaa
      541 agcgtggatt tacgcattta attgatgttt tgggagctga tcaccatggt tatattaatc
      601 gtttaaaagc tgcaattgct tttgtggggg gaaatccgga tcaagttgat atcaagattt
      661 tacaaatggt tcgggtttta caaaacggtg aggaaataaa aatgagcaag cgcagtggga
      721 aagccattac cttgcgggat ttaatggatg aagttgggac tgatgctctt aggtttatgt
      781 atgtttccaa agctttaaac acccacatgg atctagattt ggatttagcg gtccaaaata
      841 gtaatgacaa tccggtttat tatgttcaat atgcctatgc ccgcattgcc agcgtctttc
      901 gcgttgtgga agaaaaaggc tttctttttc aagcggttaa ttctttttct catcttcatt
      961 tagagtcaat cgaacaattg gttttacagc ttgtccaata tccgcttatt attgaagagg
     1021 ctgccaccaa acgcttaccg cataaattag cacaatattt gttggattta gctcaagctt
     1081 tacataccta ttataatgat gaaagaatca tcaccgataa ttgggatgaa gtaagagaaa
     1141 aattaacggt tttaaaagct gttcaaatcg tcttaaaaga cggtttaaat cttatcggtg
     1201 ttaatactaa agaaaggatg taggaggttc tatgaaagaa ttctttaaaa agtatttatt
     1261 cgtatttgaa tggattggcg ccgccatttt aattggcgtt ggcatttatg tcgttttgga
     1321 ttcttcaatt cttaatgtat ttgtcggctt aattttattg gtgtttggtt tgcttcggct
     1381 tattcctctt atcaaaacaa ctccagataa agttttgaaa attatgtatg cttgtgagat
     1441 tgtaataaat gtcggagcag gaatctttct cgttattgaa ggtggaaaag aaaattccaa
     1501 cctcaatgat tggtttggct atatgattgg tggggttttg atgttaaggg gttttgtgca
     1561 cttctttgcc acttcccttc gtaaagaacc aaacgattat gtcaaatttt tctcccacct
     1621 cggtttgttt acggtcgggt gtttcattgt tgctcgaggc ggcttttcat cggatacttt
     1681 ggcttatatt attttagtat tggcgatctt aagtgctctc tttattggtt tcagcggata
     1741 taaacattat cgcaattatc gttatgaatt acgagctaga gaagaatcaa aaaaagttgt
     1801 tgttcaagat gaaaaacaac cggaacaaat aattgaagat cctaagccaa aaaaatctaa
     1861 gattatcact ccggaagaag aaaaaaaaga tgaggccata ctatcataac aaggagaaat
     1921 aatgaatcaa gaacgccttt ttttgaaaaa attaaccgaa attgatggtg tttccggtca
     1981 tgaaaaggcc gtttcccaat ttttggaaaa agaattatta acattcgtta atcaaaatga
     2041 tgtgagtttt gatggactag gctccattgt tgctaagaaa gttggcgatg ccaatggccc
     2101 caaaatcatg atttcggggc atatggatga aattggcatg attgtcacca aaattactga
     2161 tgaaggcttc attcgttttc aaaccattgg cggatggtgg agccaagtta tgcttgccca
     2221 gcagtatgtg attacgaccg ggaagggaaa acgcattttt gcgcttatgg gttccaaacc
     2281 gcctcatttg ctaacttccg atgattttaa aaaaccagct aatattgatg agatgttttt
     2341 agatattggg gtagacaaca aagaagaagt tgaaaaactg ggaattgaga ttggggatat
     2401 gattgctccc aatattgctt ttcgcgaatt agcaaatcca caataccttt tagggaaagc
     2461 gtgggataat cgcattggtt gtgcggttgt tgtggaaacg cttcgccgat taaaaaaaga
     2521 aaatcatccc aacattgttt atggtgttgg gtcagttcaa gaagaagttg ggcttcgtgg
     2581 cgccaaaaca gcagcacaaa tgattaaccc tgacctctgt tttgctttgg atgttgggat
     2641 tgcgaaagac acaccgggaa cggatatgtc cgttaagatg gggaaaggcc ctgctatctt
     2701 agtttatgac agtgctttaa ttggtcatgt tgatctccgc aattttgtga ttcaagtggc
     2761 aaaagaggaa aatattcctt atcaacttga ttacctaaaa cggggtggaa cagatgcagg
     2821 taccgctcat ttggtccatt ctggagttcc atcgatgtcc ctttgtatcc ctactcgcta
     2881 tattcactcc cacacttcaa ttttacatta tgatgattac gaaaatgcca ttcgcttgct
     2941 ggttgctgtt attaaaaaac ttgatcatca aaccgtgaag gacattactt ttgcttaacg
     3001 ggaaaactac caacatgg
//
LOCUS       DAOSGN010000064         2993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_31184_length_2993_cov_2.792376, whole genome shotgun sequence.
ACCESSION   DAOSGN010000064 DAOSGN010000000
VERSION     DAOSGN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31184_length_2993_cov_2.792376"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PK087_01480"
     CDS             complement(<1..975)
                     /locus_tag="PK087_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH17973.1"
                     /translation="MKTNKRFFLVLFLLVLGTLFIACKPDDDVNDDPVLPTLSVTETS
                     VTLKIAETQQITATISGTTETLTPTFTSSDDAIATVSTSGLITALKEGQATITVGVSN
                     YASVTKTVTVTVEKVPEPVLPTLAVTPASVTLKVEETQQITATISGTTEALTPTFTSS
                     NEAVATVSQTGLITALVEGQATITVGVSNYASVTKTVTVTVEKADEPDVPVNPLTLTG
                     PDTVAVGQTITLIATDSGNPDNVVFWESSDTSILKVSQEGVVSGVKGGVAKVIITSFY
                     TPDTLELEITVTIPDATGIEIAPVTIPTISLSTSFKLNAKILPFGAEGE"
     gene            1092..1616
                     /locus_tag="PK087_01485"
     CDS             1092..1616
                     /locus_tag="PK087_01485"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOH17974.1"
                     /translation="MKPKWKIIIIVFAIIILIVVGLVLGNPSEPAEIFSLNQNEEQVV
                     NDLVVEIKGEVNRPGLYFLNSNSRINDAIILAGGFTTKANTAAVNLALKVTDGMVILV
                     PANDSDVITTKISINRASLNELMELNGIGEVKARNIINYRNEKGLFKTLEELLNVEGI
                     SQKLFDQIKDDICL"
     gene            1585..>2993
                     /locus_tag="PK087_01490"
     CDS             1585..>2993
                     /locus_tag="PK087_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOH17975.1"
                     /translation="MTKLKMTSAYEKLVNNWFFVAFFIMLGALALNHWWVIFLVVGFG
                     FWLWKAQKTLFYVSVVIMVIVTIIFICCCWRFQPVMDNEVTGMVVNVESKDHYQTITV
                     RKQQGFFLIYDKTNQAIKLGDIIVVEGEALMSDSARLPKGFDYRAFLFHKKIVGRISA
                     SHINVVGHRFCLPMLGAFLKQQIKKNMDEESSAFILAVLLGDDDGFDANFSQAIKDNG
                     ILHLFAISGLHISLFVGMLTTLLTRIKIKDNHQIVLITLFLVAYLIVTAFSASVFRAA
                     LMWGLGTVNKKAKLRLSSLDVLSLTFMILMIINPFYMYNLGFQLSFLATLVIIIGGPL
                     LENQASVVRLFALSCLCLLFTLPLIGNLNYRINLLSPITNVVFILLFEVIIMPASIIT
                     LLIPYLSYLYRPLVRSFKTLNGFFQTYFTLYLPLPKMELGMMIAFYFLMIVAVRLFFR
                     RQPKQIYALLLLVAILGAI"
BASE COUNT          827 a          550 c          634 g          982 t
ORIGIN      
        1 ttcaccttcg gcaccgaaag gaaggatttt cgcattcaac ttaaaagaag ttgataacga
       61 aatcgtcggg atagtaaccg gagcaatctc aataccggta gcatcgggaa tggtgacagt
      121 aatttctaac tcaagggtat cgggagtata gaacgaagta ataatgactt tcgcaactcc
      181 ccctttcacg ccgctaacta ctccttcttg ggatactttt agaatggaag tatcacttga
      241 ttcccagaag acaacattat cagggtttcc ggaatcggta gcgattaggg taattgtttg
      301 tccgacggca accgtatccg gtccggtaag agttagaggg ttaacgggaa catccggttc
      361 gtcagctttt tcaacagtca cagtcacggt tttcgttacg gaagcataat tgcttacccc
      421 aacggtaatc gtcgcttggc cttccacaag ggccgtgatt aaaccggttt gggaaacggt
      481 agccacagct tcattggaag aagtgaaagt tggtgttaat gcttcggtag taccactgat
      541 ggtagcggta atttgttgtg tttcctcaac cttcaaagtg acgctagccg gggtaacggc
      601 taaagtcgga agcacgggtt cgggaacttt ttcaacagtc acggtcacgg ttttcgtaac
      661 ggaagcataa ttactaacac cgacagtaat tgtcgcttgt ccttccttaa gggcggtgat
      721 taaaccgctt gtggaaacgg tggcaatcgc atcatcggaa gaagtgaagg ttggtgttag
      781 tgtttcagta gtaccactga tggtcgccgt aatttgttgt gtttccgcaa tcttcaatgt
      841 gacgctggtt tcggtgacgc ttaaagtggg aagcacggga tcgtcattga catcatcatc
      901 gggtttgcaa gcgatgaata atgtccctaa aactagcaga aacaaaacaa gaaaaaatct
      961 tttattagtt ttcataagct ctcctttttt ctttcattat atcatattat tttagcttgc
     1021 gaaagtattt tgactaaaaa ggaacgaaaa aaagaaaatg ccggataata tatgaatgga
     1081 ggataaataa tatgaaaccc aaatggaaaa tcatcatcat tgttttcgcc atcatcatct
     1141 tgattgtcgt tggcttagtg cttggcaatc catccgaacc ggcggaaatt ttttcgctta
     1201 accaaaatga agaacaagtt gttaatgatt tggttgtgga aataaaaggc gaagttaacc
     1261 gtccgggttt atattttctc aacagtaatt cccgcatcaa tgatgccatt atcttagcgg
     1321 gcggttttac gaccaaggcc aatacggcag ccgtcaatct ggctttaaaa gtgacggatg
     1381 gcatggtcat cctagtacct gccaacgaca gcgatgtcat cactaccaaa atctccatta
     1441 accgggcatc attaaatgaa ttaatggaat taaatggcat tggtgaagtt aaagctcgca
     1501 atatcattaa ttatcgtaac gaaaaaggct tatttaaaac ccttgaggaa ttgttaaatg
     1561 tggaaggaat atcacagaaa ttatttgacc aaattaaaga tgacatctgc ctatgaaaaa
     1621 ctagtcaaca attggttttt tgtggctttt ttcatcatgc ttggtgctct tgccctcaat
     1681 cattggtggg tcattttctt agttgttggt tttggttttt ggctttggaa agctcaaaaa
     1741 acgctttttt atgttagtgt tgtgataatg gtgatcgtca ccattatttt tatttgctgt
     1801 tgttggcgtt ttcagccggt aatggataac gaggttacag ggatggttgt taatgtggaa
     1861 agcaaggatc attatcaaac catcacggtt cgaaaacagc aaggcttctt tttaatttat
     1921 gacaaaacta atcaagcaat aaaattagga gatattattg ttgttgaagg agaagctttg
     1981 atgagtgatt cggctcgtct tccgaaaggt tttgattatc gggctttttt attccataaa
     2041 aaaattgttg ggaggataag tgcttcccat atcaatgtcg tcggtcaccg cttttgttta
     2101 ccgatgctag gagctttttt aaagcaacaa attaaaaaaa acatggatga ggaatcctcg
     2161 gcttttatct tagcggtgct tttaggagat gatgatggct ttgatgcgaa cttttctcaa
     2221 gccattaaag ataatggcat tctccatttg tttgccattt ccggtttaca tatttccttg
     2281 tttgtgggga tgttaacaac tttattaacg cgaatcaaaa ttaaagacaa tcatcaaatt
     2341 gtcttaatca ctttgttttt agttgcttat ttaattgtga cggctttttc cgcttcggtg
     2401 tttcgggctg ctttgatgtg ggggttagga acagttaata aaaaagctaa gttgcgatta
     2461 tcatcgctcg atgttctcag tttaactttt atgatattaa tgattattaa tcctttttat
     2521 atgtataatc tcggttttca gttaagtttc ttggcaacat tagttatcat catcggcgga
     2581 ccgctcttag aaaaccaagc cagtgtcgtt cgcctttttg ctttgtcatg cctatgttta
     2641 ttattcacct taccgttaat cggcaacctc aattatcgga ttaatctcct cagtccgatt
     2701 accaatgtcg tctttatttt gttatttgaa gttattatca tgccggcttc catcataaca
     2761 ttgttaattc cgtacttatc atatctctat cgacctttag ttcggagctt taaaacgctc
     2821 aacggttttt tccaaacata ttttacgctt tatctaccct taccaaagat ggaattaggg
     2881 atgatgattg ctttttattt ccttatgatt gtagctgttc gccttttttt tagacgacag
     2941 cctaagcaaa tttacgcttt actattgctg gtagccatct tgggggcgat tag
//
LOCUS       DAOSGN010000065         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_31347_length_2981_cov_3.475051, whole genome shotgun sequence.
ACCESSION   DAOSGN010000065 DAOSGN010000000
VERSION     DAOSGN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31347_length_2981_cov_3.475051"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1121)
                     /locus_tag="PK087_01495"
     CDS             complement(168..1121)
                     /locus_tag="PK087_01495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH17976.1"
                     /translation="MYFINNLLNPQLVWEEALTLWNCEMGRIFPLSPAVFKQNIRDCP
                     EINKSASGLAFDENGLIGFLFAKEFNHEGIKKYQTLGFVSFFYISKKHRNQGVGSKLL
                     EMCEQVFKQRAKTEIVIGRDYHNLFPGVPGDFNNLTRPWLEKRQYQYRRTTHDLINWQ
                     PEAHYPLLNHRYEFRLATVKDYEKMISFFERNFPGRWQFEYEEFMTKTPTDQAYLVAL
                     DGSQIIAFTRVNDRRLRLIPYNMTWAERFTNLGAIGPLGVDKDYRQQHLGYDIVCAGL
                     NELGRRGCQPIMVDWTGLLEFYQQFGFEVWKTYNYYAKQFE"
     gene            complement(1122..1844)
                     /gene="nagB"
                     /locus_tag="PK087_01500"
     CDS             complement(1122..1844)
                     /gene="nagB"
                     /locus_tag="PK087_01500"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOH17977.1"
                     /translation="MKIIKVKNYEELSQKAAQLVAKLMHKKPDATLGLATGSTPIGLY
                     KQLIAMNQEGLISFQQIKTYNLDEYCDLPQSHPQSYYSFMHEHLFDHIDILEENVHLP
                     ECECGDLQAAVDKYNQMLKKTTIDLQVLGIGRNGHIGFNEPHTPFDQETFIVRLEEKT
                     RIDNQRFFNSLDEVPKRAITMGIKNIMAAKRILLLISGKSKAETVKKLITSEPSPDFP
                     ASVLKYHKKVTVIIDEEAASLL"
     gene            complement(1887..>2981)
                     /locus_tag="PK087_01505"
     CDS             complement(1887..>2981)
                     /locus_tag="PK087_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOH17978.1"
                     /translation="YGDDPNKVAQMVIPAFKGFQDSGILPTIKHFPGHGNTSVDSHIG
                     LPRVFSSQEAVSKMELVPFKQAIKAGIDGVMISHIVYEAYDQIYPATLSQAIITDLLK
                     KELAFSGLIVTDSLTMGAIHSSFDVDTVLTQAVNAGNDLLIFCGPANLSDQRLIFKRF
                     LELVETGAISLTRVLESVTKIWQLKEKYITPFSPSAMQVNVALAESLYQRAVTKVLNH
                     RCLPLKPQEEVLILFPEIKLASLVDNETTQYQTLSSYFSQTEIIYNFTLANHKTIIEA
                     VEHYQKIILCTYNVRPDDFQVQLFASLPKDKTLVIAMRSPYDIHHLPGLKNYVCLYEL
                     SPQSLKACAQALRGEIPFNTKLPIKLDERS"
BASE COUNT          886 a          602 c          576 g          917 t
ORIGIN      
        1 ctcccgtaat ctcgactgat gagggattaa caacttcagg ttcgtcatca tcgggtttac
       61 aagcaacaaa aacaaaagcc aaaactaaga ctaatgttaa tgttatccac tttttcataa
      121 attcctcctt ttaaataatt cgcttaatta taactctttt tttattgtta ttcaaattgt
      181 tttgcataat aattatatgt tttccaaact tcaaaaccaa attgttggta gaattccaat
      241 aagcccgtcc aatcaaccat aatgggttgg caacctcgac gcccaagttc atttaaaccg
      301 gcacaaacga tatcgtaacc gaggtgttgt tgacgatagt ctttatcaac acccaaaggt
      361 ccaattgctc ctaaattagt aaaacgctcc gcccacgtca tattataagg aatgagacgt
      421 aaacgacgat cattaactct ggtaaaggca atgatttgtg agccgtccaa ggccactaaa
      481 taagcttggt ccgtcggcgt tttcgtcatg aattcctcat attcaaattg ccagcgccca
      541 ggaaaattac gctcaaaaaa actaatcatt ttttcatagt ccttaacggt cgccaagcgg
      601 aattcatatc ggtggttaag aagcggatag tgggcttccg gttgccaatt gatgagatca
      661 tgggtcgtgc gtcgatattg atattgtcgc ttttccaacc atggtctcgt taaattatta
      721 aagtctcccg gaactcccgg aaacaaatta tggtaatcgc gaccgataac gatttccgtt
      781 ttcgctcttt gtttgaaaac ttgttcacac atttctaata atttactacc tacaccttga
      841 ttgcgatgtt ttttagaaat gtaaaagaag gatacaaaac ccaaggtttg atatttttta
      901 atgccttcat gattaaattc cttcgcaaac aaaaagccga ttaagccgtt ttcatcaaag
      961 gctaagcccg aagcggattt gtttatttcc ggacaatccc gaatgttttg tttgaagacg
     1021 gctggggaaa gggggaaaat ccgccccatt tcgcaattcc ataaagttaa agcttcttcc
     1081 caaaccaact gaggatttaa taaattattg ataaaataca tttagagtag tgacgccgcc
     1141 tcttcatcaa tgatgacggt gacttttttg tgatacttaa gcacggaagc cgggaaatcc
     1201 ggtgagggct cactcgtgat tagtttttta acggtttcgg ctttggattt tccggaaatt
     1261 aacaagagaa tccttttcgc agccatgatg tttttaatcc ccatcgtaat cgctcgtttc
     1321 ggaacttcat caagcgagtt aaaaaagcgt tggttatcaa tcctggtttt ctcctccaat
     1381 ctaacaatga aggtttcctg gtcaaagggc gtatgcggtt cattaaaacc gatgtgtcca
     1441 ttgcgaccaa ttcctaaaac ctgtaaatca atggtcgttt tcttaagcat ttggttatat
     1501 ttatccactg ccgcttgtaa atcgccacat tcacattccg gtaagtggac attttcttct
     1561 aaaatgtcaa tatggtcaaa caaatgctca tgcatgaacg aataataact ttggggatgg
     1621 gattgtggta aatcgcaata ttcatccaaa ttataggttt ttatttgttg gaaggaaatc
     1681 aaaccttctt gattcatcgc tattaattgt ttgtataagc cgatgggggt gctccccgtc
     1741 gccaagccta aagtagcatc cggctttttg tgcattaact tagccaccaa ttgcgcagct
     1801 ttttgactta attcttcata atttttgact ttaataatct tcatttttca tctgtaatca
     1861 atgaaaagca aacaatacct cattgattaa ctcctttcat ctaatttaat cggcagtttg
     1921 gtattaaagg gaatttctcc tcgtaaggcc tgggcacagg cctttaagga ttgggggctt
     1981 aattcataca aacaaacata gttctttaaa cccggcaaat gatgaatatc atagggcgag
     2041 cgcatggcaa taactaaagt tttgtctttc ggtaaactgg caaagagctg aacctgaaaa
     2101 tcgtcgggtc gaacattata agtgcacaga atgatttttt gataatgctc gacggcttcg
     2161 ataatggttt tgtgattagc cagcgtaaaa ttataaatga tttccgtttg tgaaaagtat
     2221 gatgacaaag tttggtattg ggtcgtttca ttatccacca aagaagcaag tttgatttcc
     2281 ggaaacaaaa tcaggacttc ttcctggggt ttgagcggta agcaacgatg gtttaaaact
     2341 ttcgtaacgg ctcgttgata aagactttcc gctaaggcta cattgacttg catggcgctt
     2401 ggggaaaaag gggtaatgta tttttctttc aactgccaaa ttttcgtaac tgattccaaa
     2461 actcgtgtta aagaaatagc tcccgtttcc accagttcta aaaagcgctt gaaaatcaat
     2521 ctttggtcgc ttaaattggc tgggccacaa aaaattaata agtcatttcc ggcattaacc
     2581 gcctgcgtta acaccgtatc cacatcaaaa gagctgtgaa tagcccccat tgttaacgag
     2641 tcggtcacaa tcaaaccgga aaaagctagt tcctttttca ataaatcggt aataattgcc
     2701 tgtgataagg tcgccggata aatttgatca taggcttcat aaacaatatg cgaaatcatc
     2761 acaccatcaa tcccggcctt aattgcctgt ttaaagggca ctaattccat tttggaaacc
     2821 gcttcttgcg aggaaaaaac tcgtggtaaa ccgatatggg aatcaacgct ggtattgcca
     2881 tgaccgggga aatgtttgat ggtcggaagg ataccgctat cttgaaaacc tttaaaagcg
     2941 ggtattacca tttgggcgac tttgttgggg tcatcaccat a
//
LOCUS       DAOSGN010000066         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_31655_length_2963_cov_2.122765, whole genome shotgun sequence.
ACCESSION   DAOSGN010000066 DAOSGN010000000
VERSION     DAOSGN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31655_length_2963_cov_2.122765"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..887
                     /locus_tag="PK087_01510"
     CDS             84..887
                     /locus_tag="PK087_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17979.1"
                     /translation="MFLNIFTKFKEFFSQLIRKLTFDNFDVLLFGIIVGFLLCFVIYL
                     FIVLGSMRPRKDLPKLDEQAVDEQVFKLITSAKNEYTEEFSTRPLKEKTPALREIAGN
                     LIEAIAKIYYPDSSFPLYELSIDELLILNHYITDQIDSLFKGRVLAAARKVRIANILK
                     IIEVKKKFDNTKAVKLVNQKPISGTVKVALGILNVFNPKYWVKKLLIDTTMTIATNKI
                     ALTIIEIVGAETNKVYNRSVFNAEKESNLEIERTIYEIETMMEKEGEKP"
     gene            884..2908
                     /locus_tag="PK087_01515"
     CDS             884..2908
                     /locus_tag="PK087_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOH17980.1"
                     /translation="MSRKSFYIAKIDEENNASKSLDNKQKSPSESKVEGFVSPYYGRK
                     VKDKTTFPYIKYGNQGLQYQGLWEDPRPNLSEEDGETAPVRKNRIPDYLMREDITPRR
                     QKTITPTLVVSQADEPEELEELEQFAFDEENKTSSLDELSELPELNEYFSEDRPKPEA
                     KVVPESKTDLKKTPVLEKTVITYDTKQVKPATFTKKRNRSRYVAPPLNLLKRGQTIKQ
                     SDFSGVNYQKEVIDKTLRDFKIGGRVVHYTKGPTVTQFEIKLDDGVKVERVKNISRNL
                     QMNLEGKSIRIEAPIPGKATIGIEVPNIVQDKVFFGELLSNPDYLNDNNPLNVILGLD
                     ISGRSVPLDITAMPHALIAGTTGSGKSVCINCIINSIIYKAHPDDVKLVLIDPKLIEF
                     SSYEDIPHLATPVINDPKLATAALKWAVDEMQNRYELFKSCRRRDFNSYNELAATDPS
                     LKEIPYIVIIIDELADLMFVASSSVEEYIQRLAQKARAAGIHLIMATQRPSTDVIKGT
                     IKANIQTRIAFAVNSQVDSMTILDKTGADKLLGKGDMLYSDGVSETRVQGAFISLEEI
                     IAVTDYIKDNYAPNFMFSKEDLEQKMNYEEQMEVDPREDEYFDIIARFVVENDSASIN
                     RIQKKFGIGFNRAQSIMNGLEDLGVVSEGLSGRPRRVLVTIEELESLLDL"
BASE COUNT         1007 a          495 c          596 g          865 t
ORIGIN      
        1 gtctagaaaa tcacatttgc ttggttgcaa aaaaacggct tttgtagtaa aatggatgag
       61 tataattggg aaaggagcaa accatgtttt taaatatctt tacaaaattt aaagaattct
      121 tttcccaact gattcgaaaa ttgacctttg ataattttga tgttttattg ttcggaatca
      181 tcgttggttt tttgttatgc ttcgttattt atttattcat tgttttagga tcaatgcgac
      241 ctcgtaaaga tcttcccaaa ctcgatgaac aagccgttga tgaacaggtt ttcaaattaa
      301 ttacgagtgc gaaaaatgaa tatactgagg aattttcgac ccgaccttta aaggaaaaaa
      361 cgccagcact tcgggaaatc gccggaaatt taattgaagc gattgcgaaa atatattatc
      421 cggattcttc attcccgctt tatgagttaa gcattgatga actgttaatc ttaaatcact
      481 atatcactga ccaaattgat tccttgttta aaggaagggt gctagcggcg gctagaaagg
      541 ttcggattgc taatatttta aaaataattg aagtaaagaa aaaatttgac aataccaaag
      601 cggttaaact agtcaatcaa aagccgatat ccggaacggt taaagtggct ttgggaattt
      661 tgaatgtttt taatcccaag tactgggtca aaaaattatt gattgatacc accatgacaa
      721 ttgctaccaa taaaattgcg ttaacaatta ttgagattgt tggtgcagaa acgaataaag
      781 tatataatcg tagtgtcttt aatgcggaaa aagaaagtaa tttagaaatt gaaagaacaa
      841 tttatgaaat tgaaacgatg atggaaaaag aaggagaaaa gccatgagcc ggaaaagttt
      901 ttatattgcg aaaattgatg aagaaaataa cgcttctaaa tctttagata ataagcaaaa
      961 atcaccaagt gaatcgaaag tagaaggttt tgtgtctcct tattatggtc gtaaagttaa
     1021 agataaaacg actttcccct atatcaaata tggtaatcaa ggtctgcaat atcaaggttt
     1081 gtgggaagac ccccgcccca acttatcgga agaagatggg gaaactgctc ccgttagaaa
     1141 aaatcgcatt cccgattact taatgcgtga ggatattacg cctagaagac aaaaaacaat
     1201 aacgccgact ttggttgttt cgcaagcgga cgaaccggaa gaactagaag aactagaaca
     1261 atttgctttt gacgaagaaa ataaaacgag ctcattggat gaattaagcg aattgccgga
     1321 attgaatgaa tatttttctg aagatagacc caaaccggaa gcgaaagttg ttcctgaatc
     1381 aaaaacggat ttgaaaaaaa cgccagtttt ggaaaaaacg gtaattactt atgatactaa
     1441 gcaagttaaa cccgctacat tcacaaaaaa gaggaatcgt agtcgctatg ttgcgcctcc
     1501 tcttaattta ttaaaacgcg gacaaaccat taaacaaagt gatttttctg gtgttaatta
     1561 tcaaaaagaa gtcattgata aaaccttaag agattttaaa attggcggtc gagttgttca
     1621 ttacaccaaa ggtccaaccg tcacccaatt tgaaattaag cttgacgatg gtgtcaaggt
     1681 ggaacgcgtt aaaaatattt cccgcaattt gcagatgaac ttggaaggga aatccattcg
     1741 gattgaagct cccattcccg ggaaagccac aatcggcatt gaggttccca acattgttca
     1801 agataaagtt ttctttgggg aattactatc caaccctgat tatttgaatg acaataatcc
     1861 tttaaatgtg attttaggat tagatattag tggccgtagt gtgccgttgg acattacagc
     1921 aatgccccat gccttaattg ccggaaccac cggtagtggc aaaagtgttt gtatcaattg
     1981 tattattaac agcattattt ataaagccca tcctgatgat gttaaactcg tcttaattga
     2041 tccgaaatta atcgaatttt ccagttatga agatattccc catttagcaa cccccgtcat
     2101 caatgatcca aaattagcga cggccgcttt aaaatgggct gtggatgaaa tgcagaaccg
     2161 ttatgaatta tttaagtcgt gtcgtcgccg tgactttaac tcttacaatg aactggctgc
     2221 tactgatccc agtttaaaag aaattcctta cattgtgatt atcattgatg agttagccga
     2281 tttaatgttt gtggcgtcat cttccgttga agaatatatt caaagattag cccaaaaagc
     2341 tcgtgcggcc ggtattcatt taattatggc gacgcaacga ccgtctaccg atgttattaa
     2401 aggaacgatt aaagctaaca ttcaaacgag aatcgctttt gctgttaatt cacaagtcga
     2461 ttcgatgacg attttggata aaaccggagc tgataaacta ttaggcaagg gtgacatgct
     2521 ttatagtgac ggtgtcagtg aaacacgagt tcaaggtgct ttcatttcac ttgaggaaat
     2581 cattgcggta acggactata ttaaagacaa ctacgctcct aatttcatgt tctcgaagga
     2641 agatctcgaa caaaagatga attacgaaga acaaatggaa gttgatccgc gggaagatga
     2701 gtattttgat attattgccc gctttgtggt ggaaaacgac agtgcttcca ttaatcgcat
     2761 tcaaaagaag tttggtattg gttttaatcg agctcaaagc atcatgaacg gattggaaga
     2821 tttgggtgtt gtttcggaag gcttatcggg acgaccacgc cgcgttttag ttaccattga
     2881 agaattagag agcttattag atctttaaaa aaggaaaaac tatggaaata accaagaaaa
     2941 aaatgctcca aaaaatcatt tct
//
LOCUS       DAOSGN010000067         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_32630_length_2902_cov_2.549701, whole genome shotgun sequence.
ACCESSION   DAOSGN010000067 DAOSGN010000000
VERSION     DAOSGN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32630_length_2902_cov_2.549701"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2902
                     /locus_tag="PK087_01520"
     CDS             <1..>2902
                     /locus_tag="PK087_01520"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOH17981.1"
                     /translation="LTTAQKALVTNLATLEAAEATLVSLKAVAAVETLIDALPETIAL
                     SDEEQVNAARTAYDALTTDQKGKVLNYATLTAAEATITKMKADIADQEAAAAVDTLIV
                     TLPSTVELSDKVKVVEARTAYDALSEAQKNLVTTLILLTMKEERIANLEKAKIVVDLI
                     VVIPDTLTVADESKISDARTAYEALNATQKKMVTNYDRLVQKEEDLAIVKNPDLAVLL
                     PVIKEVPKQIIVDFALPTTGGVLWSYKAGEDQSFFDLESGELLKTTYAYQPVTLLAKY
                     NTTTEEVVVNFGLLEEGQKAIFYTGATTAKPDAGNTWEGFGTYDTQSAVAGFGGYKIV
                     VGNNVYFISKNAYIPISGTTENELISRTTLRPYGISAQTDYNNTGLENGIVKTYRGAA
                     ALYHNTGTVPVTFDASDTYGRCNVPNLGFGKIMFTKNEDGTYTVAKYLADHGGPGGGN
                     STGSTGVLIATLNPGDFLWTPHSWEVDYSNIGFGTRLCQMNNGVLDEGIIIEVTQYKL
                     PDPAKKVLAAINDLPTKISLADEAVVVAIRTAYDALTSEEKALVTNYAVLEAAEAEMA
                     DLKTAAQLVVDIEALPALGEITLDNETAINNLKTKYDSLSAEHQSLVSNVATLEAAIV
                     KILELKGIYQIIYVLDGGAFSGEYPQTYDVNKLPQALVKPVKEGYSFAGWYGKSDFSD
                     SPVTAVPKESSGAKTYYAKWYPAPNPISQVLSMIDTNPNQLVAIEGVIVGITLDDYFF
                     VADNTGIVYVRFKVGTFQVGDYVRVDGTATVYLESNKQYTRQISSVTSVVKLDDATHA
                     NPLAVTTAQITDLPALGTTEVLTTDEINAVKAHALPGKYLSITGYLTVQGPFSNVYLT
                     TSLDAGAPGLYVYYQSRNQEELKFLIGRQVTLYGTLYNYDGLDKWSFVYLGDLDITLN
                     DGEKEELMKEEIEAIITEGQEVMGDLPFFTASQYQNTFPGVVVAFVSNSIDV"
BASE COUNT          873 a          574 c          654 g          801 t
ORIGIN      
        1 aattaacgac tgcgcaaaaa gccctagtta cgaacttagc gacattggaa gcggcggaag
       61 cgacattagt gtcgcttaaa gcagttgcgg cagtggaaac attaattgat gctttaccgg
      121 aaaccatcgc attaagcgat gaagaacaag ttaatgccgc tcgcacagct tacgatgcct
      181 taacaactga ccaaaaaggc aaagttttaa attatgcgac ccttacagct gccgaagcaa
      241 caattacaaa aatgaaggcg gatatcgccg atcaagaagc ggcagccgct gttgatactt
      301 taatcgtcac tttaccgtca acggttgaat tgagcgataa agttaaggtt gtggaagcaa
      361 gaactgctta tgatgcttta agcgaagcgc aaaagaactt agtgacgact ttaattcttc
      421 taacaatgaa agaagaacgg attgccaact tagaaaaagc taagattgtt gttgatttga
      481 ttgtggtcat tcctgatact ttaactgtgg ccgatgaaag caagattagc gatgctcgca
      541 ccgcttatga ggctttaaat gccacccaaa agaagatggt aaccaattat gatcgacttg
      601 tccaaaaaga agaagacctg gctattgtca agaatcccga cttagcagtt ttacttccgg
      661 tcattaaaga agtaccgaaa caaatcatcg ttgattttgc cttaccgacg accggtggag
      721 tcctgtggtc ctataaagcc ggtgaagatc aaagtttctt tgatttggaa agcggggaat
      781 tgttaaagac cacctatgcc tatcaaccgg ttactttatt agctaaatac aataccacga
      841 ctgaagaagt ggtggtgaac ttcggtttac tcgaagaagg tcaaaaggcg attttctata
      901 ccggtgctac tactgccaaa cccgatgctg gcaacacttg ggaaggtttt gggacttatg
      961 acacgcaatc agccgtcgcc ggctttggcg gttacaaaat tgttgttggt aataatgttt
     1021 atttcatttc caaaaatgcc tatattccaa ttagcggaac gaccgaaaac gagcttatct
     1081 cccgtactac tcttcgtcct tacggaatat cagcgcaaac cgattataac aatacgggtt
     1141 tggaaaacgg tatcgttaag acttatcgag gagctgcggc tctatatcat aataccggaa
     1201 ccgtgccggt aacctttgat gccagtgaca cttatggccg ttgcaatgtt cctaatttag
     1261 gttttggaaa aatcatgttt accaagaacg aagatggaac ttatacggtc gcgaaatatt
     1321 tagccgatca tggtggtcca ggtggcggta actcaaccgg ttcaaccggt gtcctaatcg
     1381 ctactttaaa ccccggtgat ttcttatgga caccgcatag ctgggaagtt gactacagca
     1441 atattggttt tggaacgcgc ttatgccaga tgaacaatgg cgttttagat gaaggaataa
     1501 ttattgaagt tactcagtat aagcttccgg accccgccaa aaaagtgctt gcggccatta
     1561 atgacttacc gactaaaatt agcttagccg atgaagcggt ggttgtagcc atccgcaccg
     1621 cctatgatgc cttaacaagt gaagaaaaag ctttggttac caattatgcg gttttagaag
     1681 cggcggaagc ggaaatggcc gacttaaaga cggctgccca gttagtagtt gatattgaag
     1741 ctcttccggc gcttggggaa atcaccctag ataacgaaac ggcgattaat aatttaaaaa
     1801 ctaaatatga cagcttaagc gcagaacatc aatccttggt tagcaatgtg gcgacattag
     1861 aagcggcgat tgtgaaaatc ttggaattga agggtatcta tcaaatcatc tatgtgcttg
     1921 atggcggtgc tttcagcggt gaatatccgc aaacctatga tgtaaacaaa ttgccacaag
     1981 ctttagttaa accggttaaa gaaggatatt cttttgccgg ttggtatggc aagtccgatt
     2041 tctcggattc accagttacg gctgttccga aggaatcaag cggtgccaaa acttactatg
     2101 cgaaatggta tccggctcct aacccgattt ctcaagtatt aagcatgatt gatactaatc
     2161 ccaatcagtt ggttgctatt gaaggtgtta tcgttggtat tactttggat gattatttct
     2221 tcgtagctga taatacggga attgtttatg ttcgcttcaa ggttggaact ttccaagttg
     2281 gtgattatgt gcgcgtcgat ggaactgcga ccgtttattt ggaaagcaat aaacaatata
     2341 cgcggcaaat ttcttccgta acaagcgtag ttaaactcga tgatgctacc cacgccaatc
     2401 ctttggctgt aacgacggcc caaattaccg atttaccggc cttagggacc acggaagtat
     2461 taacgaccga tgaaatcaat gcggtaaaag ctcatgctct cccaggtaaa tacctcagca
     2521 ttaccggtta tttaaccgtt caaggtcctt tcagcaatgt ttacttaacc accagtttag
     2581 atgcaggtgc gccggggctt tatgtttatt atcaatctcg caatcaagag gaattaaaat
     2641 tcttaatcgg taggcaagta accttatatg gaaccttata taactatgat ggtttagata
     2701 aatggagttt tgtttattta ggcgatttag atataacttt aaatgatggg gaaaaagaag
     2761 aattaatgaa agaggaaatt gaagcgatta ttacagaagg acaagaagta atgggtgatt
     2821 taccattctt caccgcttcc caatatcaaa acactttccc cggtgttgtg gttgcatttg
     2881 tttcgaacag catagatgtg at
//
LOCUS       DAOSGN010000068         2888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_32845_length_2888_cov_3.902577, whole genome shotgun sequence.
ACCESSION   DAOSGN010000068 DAOSGN010000000
VERSION     DAOSGN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32845_length_2888_cov_3.902577"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2462
                     /locus_tag="PK087_01525"
     CDS             <1..2462
                     /locus_tag="PK087_01525"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOH17982.1"
                     /translation="TFYSLFDPLNLIALLLPLKWIGVEYGLFIGIRLYLAGLFFLFLL
                     QYQGIKSHKALLLATFFYVFNVTALYSAFRHPMFINGILWLPLIILGASHCLDGKKPY
                     LLLVGAFFAFITQFYLSIYAAVAFLAFVVINLYLQRKTIPKVLLFKKLFKASLWFALG
                     IFMAGFVLVPQLLAVYYGARAQSKGLLIYDSLYYLGTFSSFFIPMVSSSYTSALGNAL
                     VFFLGLVYFFQKPRDWKTIFFSFFSLMLLVSPFGYAINAFSYVNNRWSYLLLLPAGLA
                     LGEILEHHDVLQAEQYARPTKLMISFVVAMVFCLLAYLGTKWFYPSKAIIWQVFMAII
                     IVFLFVLINNPRFISSFWCRLKPLSLKWMPKVLIISYLVFGLAISLSYVFTQTTATNL
                     AAYDHQEAFDQFPDDGFYRIDQSSYVLKSDYLGNDNLVYRFKSPYAYNSMVSGAIAEV
                     IDYYQVYNLNSTVGYTGFNNRTALNAINQVRYMMIKSQDAKDIPYDFTEAFRFHLNEV
                     VNPTIANGGRLTGKLEEVIVYESEHFLPFGFVYHQYLGQTQADSMNAIERENALLTHA
                     LIKDPQAEIVYPAVNKQLSTDVLYRGIVKTGTTYLCEEGAYLSFTIPAQAGRDVYFHA
                     DLFRELDNQKRHYYVEAYENNQLVSRFRETLYAKGTYFYYDNHEPLINLGSFATNEVR
                     VKILFDPGRYEIINLGYYLNDVSDVKTKVNELKQETLQHLQFGTHGFSGDITLNEAGL
                     LFISLPYSRGFTAYVNGKESKIERVNIGYMGLYLEAGYHEITFEYKTLGMSTGLYLSL
                     ASFMVTAGLVLINSRKKRDGH"
     gene            2452..>2888
                     /locus_tag="PK087_01530"
     CDS             2452..>2888
                     /locus_tag="PK087_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17983.1"
                     /translation="MAIKRFLESTWFMVIIYAATLGFWYLKNQLWATAFSIIVGFVLA
                     IFKTKKIVYATLGLGILINYRATNFEYNQTIVYLIVGTLLPLAAISVFTNKFNFKDLI
                     LWAFLFYLGANLLSLITIPKHLLADGIVGVLQTLAFVFIYAYFR"
BASE COUNT          759 a          541 c          588 g         1000 t
ORIGIN      
        1 atacttttta ttcattgttt gatccgttaa acttaatcgc ccttttgtta ccccttaaat
       61 ggattggggt ggagtatggt ctctttattg gcattaggct ttatttggcg gggctgtttt
      121 ttttgttctt gttgcaatat caaggaataa aaagtcataa ggctcttctt ttagcgacct
      181 tcttttatgt ttttaatgtg acggcgctat attccgcttt tcgtcatccg atgtttatta
      241 acggtatttt atggcttccc ttaattattt taggagcaag tcattgttta gacgggaaaa
      301 agccgtattt attgttagta ggcgcctttt ttgcttttat cacccaattt tatttaagca
      361 tttatgcggc ggtcgctttt ttggcttttg tagtaatcaa tctgtacttg caaagaaaaa
      421 caatacccaa ggttttactc tttaaaaagt tgtttaaggc tagtttatgg tttgcgctgg
      481 gaatttttat ggccggcttt gttttagttc cccagttgtt ggcggtttac tacggggctc
      541 gtgctcaaag caaaggtttg cttatttatg atagtttata ttatttagga accttttcat
      601 cgttctttat tccgatggtc agttccagtt atacctccgc tttgggcaac gctctggttt
      661 tctttttggg gctggtctat ttctttcaaa aaccccgaga ttggaaaacg attttcttta
      721 gctttttttc gttaatgctg ttggtttctc cctttggcta tgccattaat gctttttcct
      781 atgtcaataa ccggtggagt tatcttttac tactgccggc aggattggca ctaggagaaa
      841 tcctagaaca tcatgatgtt ttacaagcgg agcaatacgc acgaccaacg aaattgatga
      901 ttagtttcgt ggtagccatg gtgttttgcc tccttgctta tttgggaacc aagtggtttt
      961 atccctccaa agccatcatc tggcaagttt tcatggccat tatcattgtc tttttattcg
     1021 ttttgattaa caatccgcgc tttatatcaa gtttttggtg ccgcttaaaa ccgctatcct
     1081 taaaatggat gccgaaagtg ttaattattt catatcttgt ttttggctta gccatcagtt
     1141 taagctatgt ttttacccaa accaccgcca ccaatttagc ggcctatgat caccaagaag
     1201 catttgacca atttccggat gatggtttct accgcattga ccaaagcagc tatgttctta
     1261 aaagcgatta tttgggtaat gacaatttag tatatcgatt caaaagcccc tatgcataca
     1321 attccatggt ttcgggagcg attgcggaag tcatcgacta ttatcaggtt tataatttaa
     1381 acagcacggt tggttatacg gggtttaata accggaccgc cttaaatgcg attaaccaag
     1441 tgcgctatat gatgattaag agtcaggatg cgaaggatat tccctatgat tttacggaag
     1501 cttttcgctt tcacttaaac gaagtcgtta atccgaccat tgcgaacggt ggtaggctga
     1561 caggtaagct ggaagaagtc attgtttacg aaagtgagca cttccttccg tttggctttg
     1621 tttatcacca atatcttggc caaactcaag ccgattccat gaatgccatt gaaagggaaa
     1681 atgccttgtt aacccatgcc ttaatcaaag acccccaagc cgaaatagtt tatccggcgg
     1741 taaacaagca attaagcacg gatgttttat atcgtgggat tgtaaaaacg gggacaacat
     1801 atctttgtga agaaggagct tacctcagct tcaccattcc tgctcaagcg ggtcgggacg
     1861 tttattttca tgctgacctt ttcagggaac tggataatca aaaacgccat tattatgtgg
     1921 aagcctatga aaacaatcaa ttagtcagcc ggtttcggga aacattatat gctaaaggga
     1981 cttattttta ttatgacaat catgaaccgc tgattaacct cgggtcgttt gctactaatg
     2041 aagtgagggt caaaatatta ttcgatcccg gtcgttatga aattattaat ctcggttatt
     2101 atttaaacga tgttagtgat gtcaaaacaa aagtaaatga actgaaacaa gaaacacttc
     2161 agcatctcca atttggaacg catggtttca gtggcgacat cactttaaac gaagccggtt
     2221 tattatttat ctctctacct tacagccgtg gttttacggc ctatgtcaac ggtaaagaaa
     2281 gcaaaataga acgagtgaat atcggctata tgggccttta tttagaagcc ggttaccatg
     2341 agattacttt tgaatataaa actttaggaa tgagcaccgg cctttatttg tcgctagcta
     2401 gttttatggt gacggccggc ttagtgttaa tcaattctag aaagaagcgt gatggccatt
     2461 aaaagatttt tagaaagcac ctggtttatg gtgattatat atgcggcgac gctcggattt
     2521 tggtatttaa agaaccaact ttgggccacg gctttcagca tcatcgtggg ttttgtttta
     2581 gcaatcttta aaaccaaaaa aatagtttat gccaccttag gcttgggaat tctcattaat
     2641 taccgtgcca ccaattttga atacaaccaa actattgtgt atttaattgt gggaacatta
     2701 ttgccattag cggcaatcag tgttttcacc aacaaattca actttaaaga tttaattctg
     2761 tgggcgtttt tgttttattt gggagccaat ctattatcgt taatcaccat tcctaagcat
     2821 ttgttggccg atgggattgt tggtgtgctc caaaccttag cctttgtctt tatttatgcg
     2881 tattttcg
//
LOCUS       DAOSGN010000069         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_33285_length_2863_cov_3.891026, whole genome shotgun sequence.
ACCESSION   DAOSGN010000069 DAOSGN010000000
VERSION     DAOSGN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33285_length_2863_cov_3.891026"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1119)
                     /locus_tag="PK087_01535"
     CDS             complement(169..1119)
                     /locus_tag="PK087_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOH17984.1"
                     /translation="MKKCLIILLLFSAIIFIGCDNDDEEVDIYTTIYPVEFLVKEIVK
                     DRLSVKSIYPRGKDVHDFESSPKDLIKMSKAKLIFHIGLGLEVFIERAKTSTLKDVPI
                     VPLSHNILVVLLNSTDLVEMMHGDVTEENVFYDPHIWLDPNKMIIMADTVLEHVIEKF
                     ASTPEDIDFFTTNANTLKTELTLIDMEFKDTLNNPNIANKTIMVDHDAYAYWTIRYGI
                     DRIKLRNDNESSDVVPQEIKQKIALAKELGIKYIVVSKNEMESSIVNQYLSELELTES
                     AKVCLHHLGTITSEEEKNGFNYFSIMRENIRLVGIIVPKQ"
     gene            complement(1129..1671)
                     /locus_tag="PK087_01540"
     CDS             complement(1129..1671)
                     /locus_tag="PK087_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17985.1"
                     /translation="MNILSFISDYWLIILFFGTAAILFLVWEIIIPIVKNQQMYKHLE
                     ACTFDNDYRLSRSFLKKADFVLEDDEEIIFVKMVMIPRKSALTINNAFTFHLFYGGLS
                     KRPGRRYPYSRYLTEVQSFLKWQPNMDKPFRKVVVLDPSTEKIQKYLNESELALVPEG
                     EKVYDYQVVSKNNFKEKILD"
     gene            1783..2490
                     /locus_tag="PK087_01545"
     CDS             1783..2490
                     /locus_tag="PK087_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOH17986.1"
                     /translation="MLKDIILPLFIVFLIIVSTFFILLLIYITKYLRISIYQKARQYT
                     ELNHRVPEGQIVFVGDSLTEFFKINEFFPNIGVYNRGIASDTTTGVLERLHDNVIVLK
                     PRKVLVQIGTNDLRARTKIPVIVNNIQKILSELKTKLPKARIYFLSLYPVNPKAVIFS
                     PFLVFPRKNKQIKKINALMKAYCEDNHFPFINIYDHLTDQKGNLKKEYTVEGLHISYN
                     GYLVIAKLLEPLLCDND"
     gene            2552..>2863
                     /locus_tag="PK087_01550"
     CDS             2552..>2863
                     /locus_tag="PK087_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOH17987.1"
                     /translation="MDNQLNLSDQELVRRAKAEELRSRGIDPFGAAFTRTANSQTINE
                     QYQNHTKEQLLELNVKAIVAGRIMTRRVKGKAGFMHIQDRYGQLQIYVRSDMLSEEQF
                     ET"
BASE COUNT          931 a          535 c          465 g          932 t
ORIGIN      
        1 ctcggagaga gtggatttac agtccaccgc gtttagccac ttcgctaccc caccatataa
       61 tctcttttac cgtcttaata ttctactaca aaagaagatt aattgcaagg atttttacaa
      121 cttttccccc tgccataaaa agcactcttc ggtaagagtg cttaacaatt attgtttggg
      181 aacaataatt ccaactaagc gaatgttttc acgcataatg gaaaaataat taaatccgtt
      241 tttttcttct tcgctggtga ttgttcctaa atgatgtaga caaactttgg cactttctgt
      301 taactctagt tccgacaaat attgattaac aatcgaagat tccatttcat ttttagaaac
      361 aacaatatat tttattccta gttctttcgc aagcgcaatt ttttgtttga tttcttgggg
      421 aacgacatcg gagctttcat tatcattgcg tagtttaatt ctgtcaatac catatcgaat
      481 cgtccaataa gcataagcat catgatcaac cataatcgtt ttgttggcaa tgttcggatt
      541 atttaaagta tctttaaatt ccatatcaat taaagttaat tccgttttca aagtattggc
      601 attggttgtg aaaaaatcga tatcttccgg cgtggaagca aatttttcaa tcacatgttc
      661 taaaaccgta tcggccatga tgatcatctt attgggatca agccaaatat gagggtcgta
      721 gaaaacattt tcttccgtta catcgccatg catcatttcc actaaatcgg tactgttaag
      781 taaaacaact aaaatgttat gacttagggg gacaatcggc acatctttca aagtcgatgt
      841 tttagctcgt tcgatgaata cttccaatcc caagccaata tggaaaatca atttggcttt
      901 ggacatctta atcaaatctt tggggcttga ttcaaaatca tgaacatctt tcccacgagg
      961 ataaatagac ttaacactta agcggtcctt aacaatttct ttaaccaaga attcaaccgg
     1021 ataaatcgtc gtataaatat caacttcttc atcgtcgtta tcacaaccaa taaaaataat
     1081 ggcactaaat aataataata taattaagca ctttttcata atatcctctt aatcaagtat
     1141 tttttcttta aagttgttct tgctgacaac ttgatagtcg taaacttttt caccttccgg
     1201 aaccaaagca agttcggatt catttaaata tttttgtatt ttttctgttg acggatcaag
     1261 aacgaccact ttgcgaaagg gtttatccat atttggttgc cattttaaaa acgattggac
     1321 ttccgttaaa taacgagaat acggataacg ccggcctggg cgtttcgata aaccaccata
     1381 aaacaaatgg aaagtaaagg cgttgttaat cgttaaggcg gatttacggg gaatcattac
     1441 catcttcaca aagatgattt cttcatcgtc ttctaaaaca aaatcggctt tttttaaaaa
     1501 acttctggat aaacgataat cattatcaaa ggtacaagct tctaaatgct tatacatttg
     1561 ctggtttttt acaatcggaa taatgatttc ccaaaccaaa aataaaatgg cggcggtacc
     1621 aaaaaaaaga ataatgagcc aataatcaga aataaaagat aaaatattca tcactttcac
     1681 cttggtttat tatacaatat taacttaatt tgtgcaaaca atatcattac aatttgttct
     1741 gtgttataat tgataaaaag tagtttgctt gggaggatta ttatgttaaa agatattatc
     1801 ttacccttat ttattgtctt tctcattatt gtttccactt tttttatttt gttgttaatt
     1861 tatatcacca agtatttgcg tatttccatt taccaaaaag cccggcaata taccgaatta
     1921 aaccaccggg taccggaagg ccaaatcgtc tttgtcggtg attccttaac cgaattcttt
     1981 aaaataaatg agttctttcc caacatcggt gtttacaacc gcggtattgc ttccgatacg
     2041 actaccggag tgttagaacg tttgcatgat aatgtcatcg ttttgaagcc aaggaaagta
     2101 ttggtccaaa ttggcacaaa cgatttacgg gcaagaacca aaatcccggt cattgttaac
     2161 aacatccaaa agattttaag tgaattaaaa actaaacttc ccaaagcccg gatttatttt
     2221 ctatcactat atccggttaa cccgaaagcg gtaatttttt cacccttctt ggtttttcca
     2281 aggaaaaata agcaaattaa aaaaatcaat gcccttatga aggcctactg cgaagacaat
     2341 cattttcctt ttattaacat ctacgaccat ctaacggacc aaaaaggtaa tttaaaaaaa
     2401 gaatatactg tggaaggtct tcatatcagt tataacggtt acttggtaat tgctaagctc
     2461 cttgaaccgc ttttatgtga caatgattag tattcattgt cttttttatt tttttttggt
     2521 ataattaata atcaaaatca gaggtgaaat tatggataat caattaaatt taagcgatca
     2581 agagttggtt agaagagcca aagcggaaga attacgcagt cgtggcattg atccctttgg
     2641 ggccgctttt accagaaccg ccaactccca aacgataaat gaacaatacc aaaaccacac
     2701 gaaagagcaa ttactggagt taaatgtgaa agccatcgtc gcgggccgca ttatgacccg
     2761 tcgggtcaaa ggcaaagccg gttttatgca tattcaggat cgttatggcc aattgcaaat
     2821 ttatgttcgc agcgatatgc tttcggaaga acaatttgaa act
//
LOCUS       DAOSGN010000070         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_33356_length_2858_cov_2.339993, whole genome shotgun sequence.
ACCESSION   DAOSGN010000070 DAOSGN010000000
VERSION     DAOSGN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33356_length_2858_cov_2.339993"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK087_01555"
     CDS             <1..230
                     /locus_tag="PK087_01555"
                     /inference="COORDINATES: protein motif:HMM:NF037640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOH17988.1"
                     /translation="LEHLLALGYTNIEEIKRLLRVGMGPCQGNTCEQIIQRELSKYLK
                     KPLEEIPTTKVRPLSIGVKIKDIFEGQEHES"
     gene            220..1365
                     /locus_tag="PK087_01560"
     CDS             220..1365
                     /locus_tag="PK087_01560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOH17989.1"
                     /translation="MRANYVIIGAGISGTSIAYHLAKAGETNIVVVDKAYLSNGATGR
                     CGAGVRQQWATKMNCLLAKASVEFFENAQNILNYPDDLEFQQEGYLIVAVNDEEDQHF
                     SKSVDLQNALGIPSRKITKAEAKQIVPHLNTDAIVSATFCPSDGHLNPFKMNDAFYRA
                     AKALGVQFYFFEEVKEIVVINQRIEKLITNKQTIETHNVINAAGGFAHEVGQLAGLDI
                     PVYAEKHEILATEPIEKIQGPMVMSFGKNLYCQQVPHGNFIMGRSTPGIKPSHSIASS
                     WSFLEEMSKTIMEILPLVGNLRVVRQWAGSYNMSPDRQPIIDEAKELPGFYMACGFSG
                     HGFMFSPTTGQLLTEIILKKPLSIDISELSLERFKNATVVAYEKLVV"
     gene            1384..>2858
                     /gene="pruA"
                     /locus_tag="PK087_01565"
     CDS             1384..>2858
                     /gene="pruA"
                     /locus_tag="PK087_01565"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOH17990.1"
                     /translation="MYEYKTEELLDFSLSQNQEAYQKALKSLPLGKTYPLIIGGKRIE
                     TSRQMISINPALISEVIGIIHQASLKEAELAMATALESFKTWSLTSPQFRADVLFKAA
                     AIIRRRKFFFSALMTKEAGKPWVEADADTAEAIDFLEYYGRQILELQHIDAKVQSRPK
                     MERNEYRYIPLGVGAIITPWNFPLAILTGMTAAAVVSGNTVLLKPASPTQVIAYHFVQ
                     VMEEAGLPAGVINFIPGRGSEIGEWIVNHPKTRFISFTGSKEVGIAIYEKAAKVQKGQ
                     IWLKRVIAEMGGKDAILVDAGVDAEAAADAIVKSAFGFSGQKCSACSRAIIHQDKYAE
                     VLDLIIKKTKALKVGNPESSDTQVGPVIDEAAFDRIMAYMAIGKKEGRLVLGGTGSKE
                     HGYFINPTVVCDVSPQSKIMQEEIFGPYLAVCSAKSFEEGLEIVNNTEYGLTGAVFSN
                     NRHHLEMARQHFHVGNLYINRKCTGAIVGYQPFGGFNMSGT"
BASE COUNT          836 a          527 c          670 g          825 t
ORIGIN      
        1 atttagaaca tttgctggcc cttgggtata ccaatattga agaaatcaaa cgcttattac
       61 gcgtgggaat gggcccttgt caaggcaata cttgtgagca aatcattcaa cgggagttaa
      121 gcaaatattt aaagaaacca ttagaagaga ttccgaccac caaagttcgc cctctttcaa
      181 tcggggtaaa aatcaaagat atttttgagg gccaagagca tgagagctaa ttatgtgatt
      241 attggagccg gaatcagcgg cacaagtatc gcctatcatt tagccaaagc tggggaaacc
      301 aatattgttg ttgttgacaa agcttattta agcaatggag caacgggaag atgtggtgcc
      361 ggtgttcgcc aacaatgggc gacgaaaatg aattgtttat tagccaaagc cagtgttgaa
      421 ttttttgaaa atgcccaaaa cattttaaat tatccggatg atctcgagtt tcaacaagaa
      481 ggatatttga ttgttgcggt caatgacgaa gaagaccagc acttttctaa aagtgttgat
      541 ttgcaaaatg ctttgggaat tccttcacgg aaaataacta aagccgaagc caaacaaatt
      601 gttccgcact tgaatacgga tgccattgtc agtgccacct tttgtccaag tgatggacat
      661 ttaaatccct ttaaaatgaa tgatgccttt tatcgggcgg ccaaggcttt gggtgtgcaa
      721 ttttatttct ttgaagaagt taaagagatt gttgttatta accagcggat tgaaaagtta
      781 attaccaata aacagacgat tgaaacacat aatgttatta atgctgcggg tggttttgct
      841 catgaagttg gacaactagc cggccttgat attccggtgt atgcggaaaa gcatgaaatt
      901 ttggcgaccg aaccgattga gaagattcaa ggaccgatgg tcatgtcgtt tggtaaaaat
      961 ttgtattgcc aacaggttcc gcacggaaac tttattatgg gtcgtagtac gccgggaata
     1021 aaacctagtc attcaatcgc cagttcttgg tcgtttttgg aagaaatgtc gaaaacgatt
     1081 atggaaatct tacccttagt tggcaatctg cgggttgttc gtcagtgggc aggatcatat
     1141 aatatgtcgc ctgaccgcca accgattatt gatgaagcca aggaattacc gggcttttat
     1201 atggcttgtg gttttagtgg tcatggtttt atgttttccc ccacgacggg ccaattattg
     1261 acggaaatca ttttgaaaaa accgctttca attgacattt ccgaattgag tttagagcgc
     1321 tttaaaaatg ctacggtggt agcatatgaa aaattagttg tgtaggagga aattaatatg
     1381 gcaatgtatg aatataaaac ggaagaattg cttgattttt cgctttccca aaatcaagaa
     1441 gcttatcaaa aagctttaaa atcccttcct ttaggaaaaa catatccttt aattattggc
     1501 ggtaaaagga tagaaactag ccgccaaatg atttccatta atccggcttt gattagcgaa
     1561 gttattggca tcattcatca agcttcctta aaagaagccg aattggcaat ggcaacggct
     1621 ttagaaagct ttaaaacatg gagtttaacc agtccgcaat tccgcgccga tgttttgttt
     1681 aaagcggcgg ccatcattcg cagacgcaaa tttttcttta gtgctttaat gactaaagaa
     1741 gcgggcaaac cttgggtgga agcggatgcc gataccgccg aagccattga ttttttagaa
     1801 tattatggtc gtcaaatttt agaattacag catattgatg ctaaggttca aagccggccc
     1861 aaaatggagc gcaatgaata ccgttatatt cctttggggg tcggtgctat cattactcct
     1921 tggaactttc ccttggcgat tttgacgggt atgacagcgg cggccgtcgt atccggcaac
     1981 accgttcttt taaaaccggc ttccccgaca caagtcatcg cttaccattt tgtgcaagtg
     2041 atggaagaag ccgggttacc ggctggggtc attaatttca tccccggaag aggctctgaa
     2101 attggggaat ggattgtgaa tcatcctaaa acccgtttta tttcctttac aggatcgaaa
     2161 gaagtgggaa tcgctattta tgaaaaagcc gcgaaggttc aaaagggtca aatttggctg
     2221 aaacgggtga ttgcggaaat gggcggcaaa gatgctattt tagtcgatgc cggtgttgat
     2281 gccgaggcgg cagctgatgc gattgtgaaa tcggcttttg gtttcagcgg ccaaaaatgc
     2341 agtgcttgtt cacgtgccat tattcatcaa gataagtatg cggaagtctt ggatttgatt
     2401 attaaaaaga cgaaggcttt aaaagtgggc aatccggaat catccgatac ccaagtcgga
     2461 ccggtaattg atgaagcggc ttttgatcgc atcatggctt atatggccat cggtaaaaaa
     2521 gagggtcgcc tagtgcttgg gggaacggga tccaaagaac atggttactt tattaatccg
     2581 accgttgttt gtgatgtttc tccccaaagt aaaatcatgc aagaagaaat ctttggtcct
     2641 tatttagctg tctgttctgc caaatcattt gaagaaggat tggagattgt caataacact
     2701 gaatatggct taaccggagc tgttttttcc aacaatcgcc atcatttgga aatggctcgc
     2761 caacatttcc atgtcggtaa tctctatatc aatcgtaaat gtacgggagc gattgtcggt
     2821 tatcaacctt ttggcggctt taacatgagc ggaactga
//
LOCUS       DAOSGN010000071         2841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_33658_length_2841_cov_2.671213, whole genome shotgun sequence.
ACCESSION   DAOSGN010000071 DAOSGN010000000
VERSION     DAOSGN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33658_length_2841_cov_2.671213"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1007
                     /locus_tag="PK087_01570"
     CDS             87..1007
                     /locus_tag="PK087_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOH17991.1"
                     /translation="MKKIVLMLLAFVSLFVLFCCKEEEYVNPKVLAVQLIPSKDTAVL
                     NAQRGPLEALLEAELDMPVQVTVATDYNALIEAMGSELVHVGLLATTAYVLAADEGYA
                     KVILKSLRYDVADDGTQLTDKPLVAGYKSQLVVHKDSGITSVAGLKGKRIAIASFTST
                     SGFVWPANLLADNNLDPEKDVTWVNAGGHDKAIQAVLNGQADAAFTFKDARALLVKTV
                     PDVREQVIFIRDTESIPNDTISVITRLDPALVEKIKQAFLNIAASEEGKAIINAVYEH
                     KGYAVATDEEYNIVRTYLNRQKEWDFGAKK"
     gene            1078..1965
                     /locus_tag="PK087_01575"
     CDS             1078..1965
                     /locus_tag="PK087_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HOH17992.1"
                     /translation="MIKFVNVSKTYANGTQALKNVNLEIFEGEFVAILGLSGAGKSTL
                     LRTVNQIIDVTEGHIYIDNIDVNTFDIYGDNPDQMIEKLTNKGLSIERDKLDLQTIDV
                     TTLKGKQLRKLRKNIGMIFQSFNIIKRMSVLQNVLTGRVAYNPTWRNILGIYKEADKQ
                     IAYRALKKVDILEKTFGRASELSGGQMQRVAIARSLAQEAKILLADEPVASLDPVTTF
                     EVMEFLKKINQEDGVTMIANLHHVDLALKYATRIVGIKGGEVVYDIPRHGKSDKQIIK
                     DLELVYGRAIGAKDFVGEH"
     gene            1965..2762
                     /gene="phnE"
                     /locus_tag="PK087_01580"
     CDS             1965..2762
                     /gene="phnE"
                     /locus_tag="PK087_01580"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOH17993.1"
                     /translation="MNLKQPEAIVLKRPSQLKKYVTIALVIIILWGSARFANFSLIDI
                     IENFHQAGRLLKQMFGKPAWNYAPKVIPPLLETIQMAIVSSLLGAIVAFPLALLASSN
                     FTENKVINQITRFILNIFRTIPSMVLASLFVAIFGSGNFAGVAALFIFSFGLISKLLY
                     ESIEAIDQGQVEAIVSLGGNKLTVLRYGIVPQILPQFISYTLYTFEVNVRAAAVLGYV
                     GAGGIGQFYERAYAFRYFDRVGIIVIITFVTVLIIDFISSQIRKGLV"
BASE COUNT          821 a          550 c          578 g          892 t
ORIGIN      
        1 aagacaaatt gttttttaaa tatttcttga tatcactcct tggcaatgct ataataaggt
       61 atcttttgaa agatggagga aaagatttga aaaagattgt tttaatgctt ttagcctttg
      121 tttcattgtt cgtcctcttc tgctgtaagg aagaagagta tgttaacccg aaagtattag
      181 ctgtacaatt aatcccttct aaagatacag ctgtgttaaa tgctcaacgc ggtccgctcg
      241 aagctttgtt ggaagccgaa ttagacatgc cggtgcaagt taccgttgct actgattaca
      301 atgccctaat tgaagccatg ggctccgaat tagtccatgt tggtttactc gcaaccactg
      361 cctatgtctt ggctgccgat gaaggctatg cgaaagtcat tctgaaatca cttcgttatg
      421 atgtcgccga tgacggcacc caattaaccg ataaaccctt agttgccggt tataaatcac
      481 aattggttgt tcataaagat agcggcatta cttcggttgc cggcttaaaa ggcaaacgca
      541 ttgcgattgc atcatttact tccacttccg gtttcgtttg gccagccaac ttattagctg
      601 ataataacct tgatccggaa aaggatgtta cttgggttaa tgccggcggt catgataaag
      661 ccatccaagc tgttttaaac ggccaagccg atgctgcttt tacctttaaa gatgctcggg
      721 cattattagt taaaacggtt cctgatgttc gcgaacaagt catctttatt agagatacgg
      781 aaagcattcc taatgacacc atttcggtaa tcacgagatt agatccggct ttagtggaaa
      841 aaatcaaaca agcattctta aatattgctg cttccgaaga aggtaaagcc attatcaatg
      901 ccgtttacga acataaaggt tatgccgttg ccactgatga agaatataac attgtaagaa
      961 cctatcttaa tagacaaaaa gaatgggatt ttggagccaa aaaataattt ttatttaaaa
     1021 atgtcttaat taatagccac caatctttgg tggctattct cataaaggag aaggaaattg
     1081 attaaatttg tgaatgtgtc aaagacatat gctaatggca cgcaagcctt aaaaaatgtt
     1141 aatttagaaa tatttgaagg tgaatttgtg gcgattctcg gtctctccgg tgccggaaag
     1201 tcaaccttgt tacgaacagt caatcaaatt atcgatgtta ctgaaggtca tatctatatt
     1261 gataatattg atgtcaatac ttttgatatc tatggtgaca accccgacca aatgattgaa
     1321 aaactaacta ataaaggttt atccattgag cgggacaaac tggatttgca aaccattgat
     1381 gtcactactt taaaaggcaa acaattacga aaactccgta aaaatatcgg catgattttc
     1441 caaagtttta atattattaa acgcatgtcg gtcttacaaa atgtcctaac cggccgtgtg
     1501 gcttataatc ccacttggcg taacatttta ggaatttata aagaagcgga taagcagatt
     1561 gcttaccggg ctttaaagaa agtggatatc ttagagaaaa ctttcgggcg ggccagtgaa
     1621 ttatccggcg gtcaaatgca aagagtggcg attgccagat cccttgctca ggaagccaaa
     1681 atcttgttgg cagatgaacc ggttgcttcc ttggatccgg ttaccacttt tgaagtcatg
     1741 gagtttttaa agaaaataaa tcaagaagac ggtgtcacga tgattgctaa tttgcatcat
     1801 gtcgaccttg ccttaaaata cgctacccga attgtcggta ttaaaggcgg tgaagttgtt
     1861 tacgacatcc cccgccacgg taaatctgat aaacaaatta ttaaagattt agaacttgtg
     1921 tacggacggg caattggggc caaagatttt gttggtgaac attaatgaac cttaagcaac
     1981 cagaagccat tgttcttaag cgtcccagtc agctaaaaaa gtatgtcacg attgccctcg
     2041 ttattatcat tctttggggc agtgcccgct ttgcgaactt tagcttgatt gatatcattg
     2101 agaactttca tcaagccggg cgtcttctta aacaaatgtt tggtaagccg gcttggaatt
     2161 atgccccgaa agtcattcca cctcttttgg aaacgattca aatggctatt gtcagttccc
     2221 tgctcggagc cattgtcgct tttccgttag ccttgctggc ttcttccaac tttaccgaaa
     2281 ataaagtcat taatcaaatt actcgtttta ttttaaatat ctttcgcacc attccttcga
     2341 tggttttagc ctccttgttt gtagctatct ttgggtccgg caattttgcc ggggttgccg
     2401 ctttgttcat cttctccttt ggcttaatct ccaaattatt atatgaatcc attgaagcca
     2461 ttgatcaagg acaagtggaa gcaattgttt cactgggagg caataaactg acggttttac
     2521 gttacgggat tgttcctcaa attcttccgc aattcatctc atatactttg tacacctttg
     2581 aagtcaatgt gcgcgctgcc gccgttttag gttatgttgg agccggaggg attggtcaat
     2641 tctatgaacg cgcctatgct tttagatatt ttgaccgcgt cggcattatt gtgattatta
     2701 cttttgtgac cgttttaatc attgacttta tcagtagtca gattcgaaag ggcttggtat
     2761 gatgaatcaa gaaatgattg ttcttcccaa aaaaccgcgt tcgaattcgc ttaagaaaac
     2821 tttgttttac attatcacga t
//
LOCUS       DAOSGN010000072         2818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_34050_length_2818_cov_3.297141, whole genome shotgun sequence.
ACCESSION   DAOSGN010000072 DAOSGN010000000
VERSION     DAOSGN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34050_length_2818_cov_3.297141"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PK087_01585"
     CDS             <1..487
                     /locus_tag="PK087_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH17994.1"
                     /translation="RGKNIADVLKMTVTEALAFFDKIPQIKNKLQTMEDVGLGYIRLG
                     QSATTLSGGEAQRVKLASELHKKITDRTIYLLDEPTTGLHSDDVKRLLEVINYIVDQG
                     ASVIIIEHNLDVIKSADYLIDLGPEGGDYGGEVIFTGTPEDILQCEKSYTGKYLKRVM
                     H"
     gene            503..1042
                     /locus_tag="PK087_01590"
     CDS             503..1042
                     /locus_tag="PK087_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH17995.1"
                     /translation="MNDQKPPVEKPDETKEDDSQEQIDHLVEELRDQLEEEGEIRFVR
                     INPKKPSWKTIIITTVLSFIFDLLLIVSINGYLEFTDSKILNIIMLSVLFSVVELILK
                     QVLNRYFFRLVISSFGLVYIPVMIVAFIIAWLFTPDLEPSSNGKVLLFFLIFMVARTI
                     MRMIFLSRNKVIEVKKVKK"
     gene            1039..2055
                     /gene="lgt"
                     /locus_tag="PK087_01595"
     CDS             1039..2055
                     /gene="lgt"
                     /locus_tag="PK087_01595"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOH17996.1"
                     /translation="MKIKRYVILTMLLLIALFLVGCSIRSGQDSLGNEITIIDRVAFT
                     IFGRNIYWYAICILIGIMGAYFYGMHVAKKLGVKENDLFDGFIWGVLIGVLGSRLYYV
                     IFSWKSGGFSEDPLRIFTGFVNEEGGLAIHGAVIAASIFTVIFCKKRKMDIYAVLELL
                     LPGFLIGQIAGRWGNFFNQEAHGGPISASVAEGRMFLEKLWLPDFIIDQMYLADNWYN
                     APIDSPMRNYMHPTFLYESAWNFIGLMILLITRKYWKKYWLGDAALFYLVWYGVGRFF
                     VESIRTDALMVTLFGTQFRQAQVISVVMVVVGIVLFILRRIYKFHPVSFVEQVELVKN
                     GHHI"
     gene            2039..2677
                     /locus_tag="PK087_01600"
     CDS             2039..2677
                     /locus_tag="PK087_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOH17997.1"
                     /translation="MVIIFDLDGTLLDSYPLIRQNFIDVFNTYLPRFCYTEELLESFF
                     GPPLIDSFLRLVDDEELAKKLVIKYREFNLKNTAKYLKAFPDALETLKTLKAKAYHLA
                     VCSNKIDEAIKEGLIIGQLDSLIDYIVGFNHVKKAKPDPEGIKLILNHFQKPGVFVGD
                     TPVDIKTGINAGIPTIGVTWALFKESILKKSGATYIARQFKDLITIMEEIHV"
     gene            2670..>2818
                     /locus_tag="PK087_01605"
     CDS             2670..>2818
                     /locus_tag="PK087_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003014924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH17998.1"
                     /translation="MFDVLIIGAGPAGLTAAIYTARNNLKVGIIEHNVPGGQMVNTAF
                     IENYPG"
BASE COUNT          798 a          464 c          634 g          922 t
ORIGIN      
        1 ccgcggtaaa aatatagcgg atgtgttgaa aatgacggtc accgaagctt tagcgttctt
       61 tgataaaatc ccccagatta aaaacaaact gcagacgatg gaagatgtcg gtttagggta
      121 tatccgctta gggcaaagtg ctactaccct ttcgggtggt gaagcacaaa gagtgaagtt
      181 agcgagtgaa ctgcataaga agattaccga ccgcaccatt tatttattgg atgaaccgac
      241 caccggactg cattccgatg atgtgaaaag attgttggaa gtcatcaatt acattgttga
      301 ccaaggggcg agtgtcatca tcattgaaca taatctcgat gtcatcaaaa gtgctgatta
      361 tttaatcgat ttaggccctg aaggtggcga ttatggtggg gaagtgattt tcaccggtac
      421 gccggaagac atcttgcaat gtgaaaaatc ctatacaggg aaatatttaa aacgtgttat
      481 gcattaacag aaaggagacc aaatgaacga ccaaaaacct ccggttgaaa aaccggacga
      541 gactaaagaa gatgattcac aagagcaaat tgatcatttg gttgaggaat taagagatca
      601 attagaggaa gaaggtgaaa ttcggtttgt tcggattaac ccgaagaaac catcctggaa
      661 aaccattatt attacgacgg tattatcgtt tatctttgac ttattattga ttgtcagtat
      721 taacggctat ttggaattta cggacagtaa aattttaaac attatcatgc tgagtgtgtt
      781 attttcggtt gtggaactaa ttttaaaaca agtcttaaac cgttatttct ttcgtttagt
      841 gatttcttcc tttgggcttg tttacattcc ggtgatgatt gtcgccttta ttattgcctg
      901 gctctttaca ccggatttag aaccgagttc caatgggaaa gttttattat tctttttaat
      961 ttttatggtg gctcggacca tcatgagaat gattttttta agtcgaaata aagttattga
     1021 agtaaagaag gttaaaaaat gaaaatcaaa cgttatgtta tcttaacgat gctcttgtta
     1081 attgcgcttt ttttagtcgg ttgcagtatt cgaagcggtc aggattcttt aggtaatgag
     1141 attaccatta ttgaccgagt ggcgtttacc atctttgggc gtaatattta ttggtatgct
     1201 atttgtatct taatcggtat tatgggcgct tatttctatg gcatgcatgt ggcgaagaaa
     1261 ttaggtgtga aagaaaatga tttgtttgac ggctttattt ggggcgtctt aatcggtgtt
     1321 ttaggatctc gcctttatta tgtgattttc tcgtggaaat cggggggatt tagtgaagat
     1381 cccttacgga tattcaccgg tttcgttaat gaagaaggcg gacttgccat tcatggcgcc
     1441 gttattgcgg cctccatttt tacggttatt ttttgtaaga aacgcaaaat ggatatttat
     1501 gcggttttgg aattattgct gccaggtttt ctcattggcc agattgccgg ccgttggggg
     1561 aacttcttta accaagaagc ccatggtggt cccatttccg catctgttgc ggaaggaaga
     1621 atgtttttgg aaaaactgtg gcttccggac ttcattattg accaaatgta tttagctgat
     1681 aattggtata atgctcccat tgattcaccg atgcgcaact acatgcaccc gaccttttta
     1741 tatgagtcgg cttggaattt tatcggctta atgattcttt taatcacgag aaaatactgg
     1801 aaaaagtatt ggttagggga tgcggcctta ttctatttag tatggtatgg tgtcggtcgc
     1861 ttttttgtgg aaagtattag gaccgatgcg ttaatggtga cactctttgg aacgcagttt
     1921 aggcaagccc aggttatttc cgttgttatg gtagtggtag gaattgtttt gtttattctc
     1981 cgccgcattt ataaatttca tccggtttcc tttgttgaac aagttgaatt ggtgaaaaat
     2041 ggtcatcata tttgatttag atggcacgct tttagatagc tatcctttaa tcagacaaaa
     2101 ttttattgat gtttttaata cctatttacc tcgtttttgc tatacggaag aattacttga
     2161 aagttttttt ggaccaccgt taattgattc gtttttacgc cttgtggatg atgaagagct
     2221 ggcaaaaaaa ctagtgataa agtatcgtga attcaatctt aaaaatacag ctaagtattt
     2281 aaaggctttc cccgatgctt tggaaacctt aaaaactttg aaagctaagg cttatcattt
     2341 ggccgtttgt tcgaataaaa tcgatgaagc gattaaagaa ggattaatta ttggccaact
     2401 tgactcctta attgattata ttgttgggtt taatcatgtg aaaaaagcga aacccgaccc
     2461 tgaaggaatc aaactcattt taaatcactt tcaaaaaccc ggggtttttg ttggtgatac
     2521 cccggttgac ataaagaccg ggattaatgc cggcatcccg acaattggcg taacctgggc
     2581 tttatttaaa gagtcgatct taaaaaaaag cggggctact tatattgccc ggcagtttaa
     2641 agatttaatt accataatgg aggaaattca tgtttgatgt tttaatcatc ggcgcggggc
     2701 cggccggcct aacggcggca atttacacgg cgcgcaataa tttaaaagtt ggcattattg
     2761 agcataatgt tccaggtggc caaatggtta atacggcctt tatcgaaaac tacccggg
//
LOCUS       DAOSGN010000073         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_34322_length_2803_cov_2.784934, whole genome shotgun sequence.
ACCESSION   DAOSGN010000073 DAOSGN010000000
VERSION     DAOSGN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34322_length_2803_cov_2.784934"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="PK087_01610"
     CDS             complement(<1..1900)
                     /locus_tag="PK087_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH17999.1"
                     /translation="MKKRNLFQNIKEILIVIGRAIKSAFRRTFFGTRKEMTLLEEEAV
                     QSPLKVTAKKFIKNKLAITGLVVFLSVILFCFVGSIFVKADIYYVNKTQANTGPGFSL
                     LSVPKELQANGILDIAVAATYSVGIDKQNNVYIWGIDINDMAEAFPGGGAAPTDIKRG
                     KELVKDLKATRVWSGNEHFILEQMDGSLVSFGLVTETISFNHTTIPTNLAVKNAMGMT
                     TYVNVVDAIAQDGGVLEVIGENQVTMVITKKNRVFMWGNTAKRPYNIAIALKNLKAVS
                     AGFSRENITFLLDSGKIKVVGTGSVASNIPEVLLGDDARGKQLITTAGSAVVLRDDGT
                     IVAWGDNLSKELDFDPRIQGHVVKLSKAAVGSDNYQHFSAVLDDGSVYSWGSTTYGAI
                     KVPKALANGTKAAHVYSSFFQNYATDADGKLLGTWGLKGHLFGTDEYGRDVFARVING
                     GRISLTIGLIAVIISTTIGIIIGGISGFYGGKVDILLMRFAEIVGSIPFFPLAMTLSA
                     ILNQVNPNFDTTDRMIMIMVILGVLSWTGLAALIRAQILSEREKEFVVAAKALGVRSR
                     AIIFKHIIPNVLTIIIINATLSYASSLLTESGLSFLGFGVQLPAPSWGNMLSGAQEMD
                     VIKSSWWRW"
     gene            complement(1904..>2803)
                     /locus_tag="PK087_01615"
     CDS             complement(1904..>2803)
                     /locus_tag="PK087_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH18000.1"
                     /translation="ARVMAYVDQSLKQSDPDLYVLKMKEAVIELGIGQPLILQYFMWL
                     KETLQGNFGYTLYTHVKVLQYIKAPMLNTIKLNIFVLVCVFAITFPLGIKTAVKKYST
                     FDNSVQVFTIIGMSLPSFFIALISILVFSAWLGILPSSGSNSGIIVRETMTKFQILLD
                     DLKYMILPVFVLTVSSLAGITRYIRGTMIDILTQDYIRTARAKGLREKVVIYSHAFRN
                     AMIPVVTIMTGWLLGIFGGSTITETIFAWKGMGKTLYDALMQGPDYAVIFAMNLFYSL
                     LGLTGNLLLDLLYTVVDPRVKLG"
BASE COUNT          914 a          621 c          489 g          779 t
ORIGIN      
        1 cccaacgcca ccaagaagat ttaataacat ccatttcctg agcaccgctt aacatattac
       61 cccaagatgg agctggcaac tgaacaccga atcctaggaa tgataatccg gattccgtta
      121 atagcgagct ggcataactt agtgttgcat taataataat aatggttaaa acgttaggaa
      181 tgatatgttt gaaaatgatg gctcgtgaac gaacgcctaa agctttggcc gccacaacaa
      241 attctttttc acgttcggaa agaatttgcg cccgaatcaa agccgctaac ccggtccaac
      301 tcaaaacacc taagataacc ataatcataa tcattctatc cgtggtatca aaattgggat
      361 tgacttgatt taaaatcgcc gataatgtca tcgctaaagg gaagaaggga attgacccaa
      421 caatttccgc aaaacgcatt aacaaaatat caacttttcc accataaaaa ccggaaatac
      481 caccaatgat aataccgatg gttgtggaaa taatcaccgc aatcagacca atggttaaag
      541 aaattcttcc accattgata acccgagcaa aaacatcgcg tccatattca tccgtgccga
      601 acaagtggcc ttttaatccc catgttccta atagttttcc atcagcatcc gtagcataat
      661 tttggaagaa actagaataa acatgagccg cttttgttcc attggctaaa gctttgggaa
      721 ctttgatagc tccgtaggtt gtgcttcccc atgagtaaac acttccgtca tctaaaacag
      781 cggagaaatg ttgataatta tccgaaccaa cagcggcttt ggagagtttc acaacatgac
      841 cttgaattct tggatcaaag tcaagttctt tggataagtt gtcgccccaa gcaacaatgg
      901 ttccgtcatc ccgaagaaca acggcactcc cggccgtggt tataagctgt ttaccccgag
      961 catcatcacc caataaaact tccggaatat tggaagcaac tgaaccggtt cctacaacct
     1021 taattttgcc actatctagc aagaaggtaa tattttcccg ggaaaaacca gccgacacag
     1081 ctttaagatt ttttaaagca atcgctatgt tgtatgggcg tttagcagta tttccccaca
     1141 taaatacgcg gtttttcttg gtaataacca tcgttacttg gttttcccca atcacttcta
     1201 aaaccccacc atcttgggca atggcgtcca caacattaac ataagtcgtc attcccatgg
     1261 cgtttttaac ggctaaattg gtcggaatcg tcgtatgatt aaaggaaatt gtttccgtca
     1321 ccaagccgaa tgataccaag gaaccatcca tttgttctaa aataaaatgt tcattaccgg
     1381 accaaacgcg ggtcgctttc aaatctttta caagctcttt ccctcgttta atatcggtcg
     1441 gagcggcacc tccgccagga aaggcttccg ccatatcatt aatatcaatt ccccaaatgt
     1501 aaacattgtt ttgtttatca atccctacac tgtaagttgc cgcaacggca atatctaaaa
     1561 tgccgtttgc ttgtagttct ttagggacac ttaacaaaga aaatccgggg ccagtattgg
     1621 cttgcgtttt gttaacataa taaatatcgg ctttcacaaa aatgctacca acaaaacaaa
     1681 aaagaatcac ggataaaaag acaactaagc cggtaatggc caatttattt ttaataaact
     1741 tttttgcggt aacctttaat ggactttgaa cggcttcttc ttccaaaagc gtcatttcct
     1801 tgcgcgttcc gaagaaagtg cgacggaatg ccgatttaat ggcacggcca ataacaataa
     1861 gaatctcttt tatattttga aataaatttc tttttttcat attctacccc aacttaaccc
     1921 gcggatcaac gaccgtgtag agtaagtcta ataataaatt acccgttaag ccaagcaatg
     1981 aatagaagag attcattgcg aaaatgacgg catagtccgg tccttgcata agggcgtcat
     2041 ataaggtttt ccccattccc ttccaagcaa aaatggtctc agtaatcgtc gaaccgccaa
     2101 atattcctaa taaccaccca gtcataatcg tcaccactgg aatcatggca ttgcggaagg
     2161 cgtgagagta aataacaact ttttcacgta atcctttggc tctcgccgta cgaatataat
     2221 cttgtgttaa aatatcaatc atcgtccccc gtatatagcg agttatcccg gccaaggaag
     2281 ataccgttaa cacgaatacc ggtaaaatca tgtatttcaa gtcatccaaa agaatttgga
     2341 attttgtcat tgtttcacga acaataatcc cggagttact tccgctactt ggtaaaatac
     2401 ctaaccaagc agagaaaact agaatcgaaa ttaaagcaat aaaaaaactt ggaagcgaca
     2461 ttccaataat ggtaaagact tgaacactgt tatcaaaagt agagtatttt ttgactgctg
     2521 ttttaattcc caagggaaaa gtaatcgcaa aaacacagac taagacaaaa atgtttaatt
     2581 taatcgtatt aagcattggt gcttttatat attgtaagac tttaacatgg gtatataaag
     2641 tataaccaaa attaccttgt aatgtttctt tcagccacat aaagtattga agaatcaaag
     2701 gctgcccgat tcccaattca attacggctt ccttcatttt taaaacatat aaatccggat
     2761 cggattgctt taatgattga tcaacataag ccataacgcg ggc
//
LOCUS       DAOSGN010000074         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_34508_length_2792_cov_3.628060, whole genome shotgun sequence.
ACCESSION   DAOSGN010000074 DAOSGN010000000
VERSION     DAOSGN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34508_length_2792_cov_3.628060"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PK087_01620"
     CDS             <1..554
                     /locus_tag="PK087_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18001.1"
                     /translation="SLHYHISKIAMITMAHYAWNSGRYDYTKSFDRALKSVSPTFASL
                     GQAFFDYNYPNVLSHGQLLIEKQMVEKHDYPAIKHYMKAVADSARQLLKLNEPIIEEL
                     SPWLKRVIEEEKWVNYIIKAEVDKAKLLVFLENIKFSGGKILDYLLIEKKILTEEEFT
                     KLISKRRGGEWYRVFEYKRWPNH"
     gene            568..1233
                     /locus_tag="PK087_01625"
     CDS             568..1233
                     /locus_tag="PK087_01625"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HOH18002.1"
                     /translation="MLEKLKNRLIVSCQAKEGEPLRDPIIMAKMATAALIGGAGAIRA
                     AGLDDIKAIKAFNDTIVIGLIKKDYPDSEVYITPTKQEVRKLLESGCEIIALDGTNRR
                     RPKQESLVGLISLIHAENRLAMADISNFEEAVMAQQAGADFVSTTLSGYTAYTKKQRG
                     PDLKLLSKCVKKLQIPVLAEGRIRNPRDLKRVLAKKPFAVVIGTAITRPSEITKTFVE
                     CFK"
     gene            1230..>2792
                     /locus_tag="PK087_01630"
     CDS             1230..>2792
                     /locus_tag="PK087_01630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH18003.1"
                     /translation="MKRKTITASQDDILVYWNQEFGSSYPLTKRLFQEKILDSKELVD
                     DGSYSLYYQEEYIGSMVLKFNHSNDKKLQDHAYLAFLFVNPKFRNQGYGSLLISDAIR
                     ICTAKEKAVLYIGGDNDCLFSGLFLLNNDHVHQFFKNHRFNIAYKNYNLICREPPALS
                     VDNYEYTTLKTEQERDDLLKQIRKLFPIRWYNEVMNCEPEAFFVAKEKHKIVGFVRIN
                     TPHFSKIANSVNTYPLYPHLGGIGPVAIFPEKKGSELANNIVKQAVNHLFTLGCSDVI
                     VDWTNFIDYFKASGFSEISNSYVLYELRLYKKHQDLQKHRYQKIENDLRHDILEGKYK
                     HGDIIPSEVELAAYYKVTRMTVRQAINNLLTDGLVYRHKGRGTFVTYNKQNIDFDDNP
                     LFSFANEQNSKGDFQIRVLVFEKRAADELLALRLQLKIGDLLYYVERMVTLDNKPMLF
                     ERMYLPANMYEDLQASIFKGSFKQYAKEQLHWHSLSCQMTVEARGLSGRAAEVLHLSV
                     GSPALYCSTLIHL"
BASE COUNT          880 a          491 c          580 g          841 t
ORIGIN      
        1 caagccttca ttatcacatt tcgaaaattg cgatgattac tatggcccac tatgcttgga
       61 acagcggccg ttatgattat acaaaatctt ttgaccgagc tttaaagagt gttagtccta
      121 catttgctag tttaggacaa gctttctttg attataatta tccgaatgtt ttgtcccatg
      181 gacagttact aatagaaaaa caaatggtgg aaaagcatga ttatcctgcc attaagcatt
      241 atatgaaagc tgttgcggat agcgctcgcc aattgttaaa gcttaatgaa ccgattattg
      301 aagagttaag cccgtggctt aaaagagtga ttgaggaaga aaaatgggtc aattacatca
      361 ttaaagctga ggtcgataaa gctaagttac tggttttttt agaaaatata aaattttcgg
      421 gcggaaagat tcttgattac ttgcttattg aaaaaaagat cctaacggaa gaagagttta
      481 caaagttaat ttccaaacga agaggtggag aatggtatcg cgtttttgag tacaaacgct
      541 ggcccaatca ttaaaatgaa aacattaatt ttggaaaaac taaaaaaccg cttaattgtt
      601 tcctgccaag ctaaggaagg tgaaccgcta agagacccaa taataatggc taaaatggcg
      661 accgccgctt taattggtgg ggccggggcc atccgggcag cgggtcttga tgatatcaaa
      721 gcgattaaag ccttcaatga taccatcgtt atcggtttaa ttaagaaaga ttatccggac
      781 tcggaagtct acattacgcc aacgaagcaa gaggttagga aacttttaga aagtggctgt
      841 gaaattattg ctttggacgg aacgaaccgt cgtcgcccca agcaagaatc cctggttggc
      901 ttaatttcgc tcattcatgc tgaaaatcgt ttggcaatgg ccgatatttc aaactttgaa
      961 gaagcggtga tggcccagca agccggagcg gatttcgttt ccacaaccct ttccgggtat
     1021 actgcttata ctaaaaaaca acgaggaccg gatcttaaat tacttagtaa atgtgtgaag
     1081 aaattacaga ttccggtgct tgcggaagga agaattagaa acccgcgtga tttaaaacgc
     1141 gtgttggcga aaaaaccctt tgcggtggtc attggaacag ccatcactag gccgagtgaa
     1201 attaccaaaa cttttgtgga gtgttttaaa tgaagcgaaa gaccattact gcttcccaag
     1261 acgatatttt agtttattgg aatcaggaat ttggttcaag ctaccccctt actaaacgtt
     1321 tatttcaaga aaaaatcttg gattccaaag aacttgttga tgacggctcc tattctcttt
     1381 attatcaaga agaatacatc gggagtatgg ttttaaaatt taatcatagc aatgataaaa
     1441 aactacagga ccatgcctat cttgcgtttt tgtttgttaa tcctaaattt cgtaatcaag
     1501 gttatggttc gcttttaatc agcgatgcca tccgtatttg caccgccaag gaaaaagccg
     1561 tactgtacat cggtggtgac aacgattgtt tgttttccgg attgttttta ttaaacaacg
     1621 accatgttca tcaatttttc aaaaatcatc gttttaatat cgcttataaa aattacaatt
     1681 tgatttgtag ggaaccacca gcgttatcgg tggacaatta tgagtatacg actttaaaaa
     1741 cggaacaaga acgcgatgat ttactgaaac aaatccggaa actgtttccg attcgctggt
     1801 ataatgaggt tatgaattgt gagccggaag cttttttcgt tgctaaagag aagcacaaga
     1861 ttgtgggatt tgtgcgcatt aacacccctc atttttcgaa aattgccaat agtgtcaata
     1921 cttatcccct ctatcctcat ttaggcggga ttggaccggt ggccatcttt cctgaaaaaa
     1981 aaggctccga attagctaat aatattgtta aacaagcggt caaccattta tttactttgg
     2041 gctgttcgga tgtcattgtc gactggacca atttcattga ttattttaaa gcctcgggtt
     2101 tttcagaaat cagtaattct tatgttttat atgaacttcg cctttataaa aaacatcaag
     2161 atttgcaaaa acatcgctat caaaagattg aaaatgattt gcgtcacgat attttggaag
     2221 gaaaatataa acacggcgac attattccca gtgaagttga attggccgct tattataaag
     2281 tcacaagaat gaccgtccgt caagccatta acaatttact aacggatggt ttagtttatc
     2341 gccataaagg gcggggaaca tttgttactt ataacaaaca aaatatcgat tttgacgata
     2401 accccttatt ttcctttgca aacgaacaaa atagtaaagg cgattttcaa ataagggtat
     2461 tagtatttga aaagcgtgcg gcggatgaat tattagcttt acgtttacaa cttaaaatag
     2521 gtgatctttt gtattatgtg gaacgaatgg ttactttaga taataaaccg atgttatttg
     2581 aacgaatgta tttgccggct aatatgtacg aagatttgca ggcatcgatt tttaaaggct
     2641 cgtttaaaca atatgcgaaa gaacaattgc attggcattc tttgtcttgc caaatgacgg
     2701 ttgaagcaag gggattaagt ggtcgggccg cggaagtttt gcatctttcc gtaggaagtc
     2761 cggcgctata ttgcagtact ctaatccact tg
//
LOCUS       DAOSGN010000075         2780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_34721_length_2780_cov_3.350459, whole genome shotgun sequence.
ACCESSION   DAOSGN010000075 DAOSGN010000000
VERSION     DAOSGN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34721_length_2780_cov_3.350459"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="PK087_01635"
     CDS             complement(<1..1270)
                     /locus_tag="PK087_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH18004.1"
                     /translation="MKNYDCIIVGAGPMGIFCAYELMHQRPKARILLIDKGNDIYHRQ
                     CPILLKKVEKCPSHANGEIGCKPACFMTTGFGGAGAYSDGKYNITTEFGGWMQEYMDD
                     NQLMELINYVDQVNIKHGANLDITNPDTPAVREIERKAIAVGLKLLRAKVRHLGTEEN
                     INILKNIYEDMKGHVDYLFAKKVISVLVEEGEAKGVVLENGQEIHGQNVVLGVGREGA
                     MWLQTTLESLGVKFKNNQVDIGVRVETNNIVMEEINEHLYEGKFKFNTSVGTTVRTFC
                     SNPSGHVVVENQNGTILCNGHAFRDLRLGTDNTNFALLVSHEFGEPFDNPNEFAHEVA
                     KLANKLSGGSVIVQRYGDIIRGRRSTPKRLKECFVTPTLKEAVPGDLGLALPYNTMKS
                     LIEMMQALNYVTPGIASDHTLFYGVEAKFYS"
     gene            complement(1370..>2780)
                     /locus_tag="PK087_01640"
     CDS             complement(1370..>2780)
                     /locus_tag="PK087_01640"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOH18005.1"
                     /translation="FVTDDGAETDLDLGHYERFVDENLNKYSSVTMGKIYLRVIEKER
                     QGKYSGGSVQVIPHITNEIKEAINISATKSKADVVIIEIGGTVGDIESLPCLEAIRQF
                     RRDVGYHNTLYIHNTLVPYIAASGELKTKPTQHSVKELRSLGINPDAIILRSDRKISD
                     SQKDKIALFCDVHKEAVIDVRDCKSIYEVPPMLAKQKIDDFICRHFGLQTPPLDLTEW
                     LKMLENIKNAKGLVKIALVGKYVSLKDAYLSVYESLKHSAFANNVNIRIDSIDAKTLN
                     ANNVGEVLNSYQGIICPGGFGDRDIDGIMFAIKYAREQHIPYLGICYAMHLAMIEYAR
                     HVLAYPKATSREFDEDADLAIIDLNPLRKGFEKPMRVGLYSCHLKPGSKLAEIYQTNL
                     IYERHRHRYEVNNATLHLFEGSDMVFSGINEEEKIVEALELKNHPWFMACQFHPEFLS
                     RPNRAHPLFLAFIQAAMKM"
BASE COUNT          780 a          661 c          518 g          821 t
ORIGIN      
        1 cactatagaa tttagcttcc acaccataga acaaagtgtg gtcgctggca atgccgggcg
       61 tcacataatt taaggcctgc atcatttcaa tcagtgattt catggtattg tagggaaggg
      121 ctaaacccaa atccccggga acagcttctt ttaaagtggg cgtaacaaag cattccttca
      181 ggcgtttcgg cgtagagcgg cgcccacgaa taatgtcacc atatctttgc acaatgacgg
      241 aacccccgct taatttatta gcgagcttgg ctacttcatg agcaaactcg ttcggattat
      301 caaagggttc cccgaattca tgggaaacaa ggagcgcaaa gttggtattg tccgttccta
      361 agcgtaaatc gcgaaaggca tgaccgttgc aaagaatcgt cccgttttgg ttttccacaa
      421 ccacatgccc gctcggattg gaacaaaaag tccgaaccgt cgttcccaca ctggtattaa
      481 atttaaattt cccttcataa aggtgctcgt taatttcttc catgacgata ttattggttt
      541 ccacacgcac accgatatca acttgattgt ttttaaattt cactcctaag gactccaaag
      601 tggtttgtaa ccacatggct ccttcccgac caacgcccaa aacgacattt tgaccatgga
      661 tttcctgacc gttttccaac actacaccct tggcttcgcc ttcttccaca agcacactaa
      721 tcactttctt cgcaaacaaa taatcgacat gtcctttcat gtcttcatat atgtttttta
      781 aaatgttgat gttttcttcc gttcccaagt ggcggacttt ggcccgtaat aactttaagc
      841 cgaccgcaat cgcttttcgt tcgatttccc gaaccgccgg cgtatcggga ttagtaatat
      901 ctaaattggc gccatgttta atattgactt gatcgacata attgatgagt tccatcaatt
      961 gattatcgtc catatattct tgcatccaac ccccgaattc agtggtaata ttatatttgc
     1021 catccgaata cgcaccggcc ccgccaaaac cggtcgtcat aaagcaagcc ggtttacaac
     1081 cgatttcacc gttggcatga ctgggacatt tttccacctt ttttaaaaga atcggacatt
     1141 ggcgatggta gatatcattc cctttgtcaa ttaataagat tcgggccttt ggcctttgat
     1201 gcattaattc ataggcacag aagatcccca ttggacccgc accaacaata atacaatcgt
     1261 aatttttcat attccgtcct cttaacttta ttttttcctt gtttatttca taagccaagt
     1321 cattttatca taaaagtttt ccaaagcaaa gaaaactaat tttgaaactt tacattttca
     1381 tagccgcttg aataaaggcc aaaaagagcg gatgggcacg attaggacgc gacagaaatt
     1441 ctgggtggaa ctggcaagcc ataaaccaag gatgattctt taattccagc gcttccacaa
     1501 tcttctcttc ttcatttatt cctgaaaaaa ccatgtccga cccctcaaaa agatgtaaag
     1561 tcgcattgtt gacttcataa cgatggcggt ggcgttcgta aataaggttg gtttggtaaa
     1621 tttccgccaa tttactgccc ggttttaaat ggcaggaata aagtccaacc cgcatcggtt
     1681 tttcaaaacc cttcctaagg ggatttaaat cgatgatggc caaatcggca tcttcatcaa
     1741 attcccggga agtggccttg ggataagcca aaacatggcg ggcatattca atcatcgcca
     1801 agtgcatagc atagcaaatg cctaaataag ggatgtgctg ttcacgggca tatttgatgg
     1861 caaacatgat tccgtcaata tcgcggtcgc caaagccccc cggacaaata atcccttgat
     1921 agctgtttaa tacttcacca acattatttg catttaaagt tttcgcatcg atggaatcaa
     1981 ttctaatatt gacattattt gcgaaagcgc tatgtttcaa actttcatag acggacaaat
     2041 aagcatcttt caaggaaaca tattttccca ccaaagcaat tttaactaaa cctttcgcat
     2101 ttttaatgtt ttccaacatc ttcagccatt ccgttaaatc aagcgggggt gtttgcaagc
     2161 caaaatgccg gcaaataaaa tcatcaatct tttgtttagc caacatgggt gggacttcgt
     2221 aaatcgactt gcaatcccga acatcaatca ccgcttcttt gtgaacatca caaaacaggg
     2281 cgattttatc cttttggctg tcgctgattt tccggtcgct ccgaagaata atcgcatcgg
     2341 gattaatccc caaggagcgt agttctttaa cgctgtgttg cgtcggtttc gtcttcagtt
     2401 ctcccgaagc ggcaatatag ggaaccaagg tgttatgaat ataaagcgtg ttgtgataac
     2461 caacatcccg ccgaaactga cgaatggctt ctaaacaggg taatgattca atatcaccga
     2521 ccgtcccgcc gatttcaata atgaccacat cggccttgct tttggtggcc gatatattaa
     2581 tggcttcttt aatttcattg gtaatatggg gaataacttg aacgcttccg cccgaatatt
     2641 ttccttgccg ttctttttca atcacccgca aataaatttt ccccattgtc acactggaat
     2701 atttatttaa attctcatca acaaagcgtt catagtgacc gaggtccaaa tcggtttccg
     2761 ccccgtcatc agtcacaaaa
//
LOCUS       DAOSGN010000076         2776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_34803_length_2776_cov_3.077913, whole genome shotgun sequence.
ACCESSION   DAOSGN010000076 DAOSGN010000000
VERSION     DAOSGN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34803_length_2776_cov_3.077913"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>666
                     /locus_tag="PK087_01645"
     CDS             <1..>666
                     /locus_tag="PK087_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOH18006.1"
                     /translation="DYFYPYEAIKDENEQAQFQASNFTSLGDYRRHQVNLLIKLMHEQ
                     LSKLPKKVEFGISPFGIYRTNTKWFKEPNESAWEQGSDNHFSCFNCYSGLFADIYLWM
                     KEGWIDYVVPQNYFDFDYWKEDAKGHLYEVVKYADLAKWWSWAAQETKVKLYMGMGLY
                     RYSKEGNWSNPEEIINQLKYNHTLKNNLGVVFFTYKNLVSEDIPALVRAKERLKQLWT
                     KAA"
     assembly_gap    667..676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            832..2709
                     /locus_tag="PK087_01650"
     CDS             832..2709
                     /locus_tag="PK087_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOH18007.1"
                     /translation="MARIYPVVKQMQRRDGAFHKPVKDLKDLFFFFDYPFDGPVDTVE
                     KPYGAMAKKRDLADVVFQKGESLSLEGYEITVSPALIIIRSPSLAGVFYATLTLQQMV
                     NQGPNLPCVVINDAPDLKIRGLMLDISRNKVPTLATLKNIIVMMSQLKMNHLELYVEG
                     FSFGYPSFTKYLEADGFISVEEYQELETYANAHFIDLVPNQNGFGHMAKWLATEEFKD
                     LAEQPEGIFLWGRHRPPSTLNPLDPQSLELIKTLYRDMLSIARSKYFNMNFDEPFELG
                     KGRSKTACEEKGLGNVYIDFVLKAYAEIKKYQKTPLIWGDVLIHHPELLHRLPSDMLF
                     IDWGYDGNYPFSAHLQKLHDLGIKFLAAPGTSSWCSFLGRKDDWLENITNACLQVKTL
                     NGEGIILTDWGDFGHLQFLPISYAPLVLAGLMSWRVQEGTQFLLRDYLNQYVFLDQAN
                     IMADTIIDLGLYYHYLNEYRSNGTTTFHNFMWATVAVKEKNPIEYFLERSKTTILSSS
                     KHALLEQFLALQTKALTQTKMVDGQLIVSELKQSIQLVLLIQYLNQSFNEELSIKERL
                     SFVQKILDAETAFLAQQEELWLARNKAGGLKDSLEYLHKFFLFSQQTKKHLQRGGAYG
                     A"
BASE COUNT          849 a          517 c          572 g          828 t
ORIGIN      
        1 tgattatttc tatccctatg aagccattaa agatgaaaat gaacaggccc aattccaagc
       61 atctaacttt acaagcttgg gtgattatcg tcgtcatcaa gttaatttac tgattaaatt
      121 aatgcatgag caattatcaa agcttcccaa aaaagttgaa ttcgggatta gtccgtttgg
      181 gatttaccgc acgaacacca agtggtttaa agaacccaac gaatcagcgt gggagcaagg
      241 gtccgataat catttttcct gctttaattg ctatagtggc ttatttgcgg acatttatct
      301 atggatgaaa gaaggctgga ttgactatgt tgtgccccaa aattactttg attttgacta
      361 ttggaaagaa gatgcgaagg gtcatttata tgaagtagtt aaatatgcgg acctggccaa
      421 atggtggtca tgggcagccc aagaaacgaa ggttaagctg tacatgggca tgggtttata
      481 ccgctattca aaggaaggca actggagcaa cccggaagaa atcattaacc aacttaagta
      541 taatcacact ttaaaaaaca atttaggcgt cgttttcttt acctataaaa acttagttag
      601 cgaggatatt cccgctttag ttagggctaa agaacgctta aaacaactat ggaccaaagc
      661 tgcccannnn nnnnnncaag aaatttaacc aaagagctcc gatttatttc ggagcttttt
      721 tcttaaactt caggttaatt ttggatttta ccttgaaaaa aaacggttgg cattgtataa
      781 tgagaagaag taaaatcggt aaaaattgct tatttgcata aggagaagca gatggctcgg
      841 atttatcctg ttgttaaaca aatgcaaagg cgcgatggtg ctttccataa acccgtcaag
      901 gatttaaaag accttttctt tttctttgat tatccctttg atgggcccgt tgatacggtg
      961 gaaaagcctt atggtgcgat ggctaaaaag cgtgatttag cggatgtcgt tttccaaaaa
     1021 ggtgaatctt tatcccttga aggctacgaa atcacggttt cccccgcgct aatcatcatt
     1081 cgatcccctt cgttagccgg ggttttctat gcaactttaa cgcttcagca gatggtaaat
     1141 caaggtccga acttgccttg tgttgtgatt aatgatgctc ccgatttaaa aattcgaggt
     1201 ttgatgctcg acatcagtcg caacaaggtt ccgaccctcg ccacgcttaa aaatatcatc
     1261 gtcatgatga gtcagttaaa gatgaatcat ttagaattat atgtggaagg attcagtttt
     1321 ggctatccct cctttaccaa gtatttggaa gctgatggct ttatttccgt tgaagaatat
     1381 caagaactgg aaacctatgc caatgctcat ttcattgatt tggtgccgaa ccaaaacggt
     1441 tttggtcata tggcaaaatg gctggcgact gaagaattta aagatttggc ggaacaaccg
     1501 gaagggattt tcttatgggg acggcatcgt ccgccgagca ctttaaatcc cctcgacccc
     1561 caatctttag aattgattaa aactttatat cgagatatgt taagtattgc gcggtcgaaa
     1621 tatttcaata tgaattttga tgaacccttt gaactgggga aaggtcgttc caagaccgct
     1681 tgtgaagaaa agggtcttgg caatgtttat attgattttg tattaaaagc atatgcggaa
     1741 atcaaaaaat accaaaaaac gccgttaata tggggagatg tcttaatcca tcatcccgaa
     1801 ctactacatc gcttgccttc cgatatgctg tttattgact ggggctatga tggcaattat
     1861 cccttttctg ctcacttaca aaaattgcat gatttaggaa tcaaattcct agccgccccg
     1921 ggaacaagtt cttggtgcag tttcctagga cgaaaagacg actggttaga aaacatcacc
     1981 aacgcttgtt tacaagtaaa gactttaaat ggcgaaggca tcattttaac ggattggggc
     2041 gatttcggtc acctgcagtt tttaccaatc agctatgccc ctttggtgtt ggccggctta
     2101 atgagctggc gcgttcaaga aggaacccag tttttattaa gggattacct caatcaatat
     2161 gtgtttttgg atcaggccaa tatcatggcg gatacaatca tcgatttagg tttgtactat
     2221 cattacttaa atgaataccg aagtaacggc actacgacct tccataattt catgtgggcg
     2281 acggttgcag ttaaggaaaa aaatcccatt gaatatttcc ttgagagatc aaaaacaaca
     2341 attttgtctt cttcaaaaca tgctttgtta gaacaatttt tggctttgca aacaaaagcg
     2401 cttacccaaa ccaagatggt ggacggccaa ttaattgttt ccgaactgaa gcaatccatt
     2461 cagttggtgc ttttaattca atatctcaat caaagcttca atgaggaatt atcgattaag
     2521 gaacgactaa gctttgttca gaagattttg gatgcggaaa ctgctttttt ggctcaacaa
     2581 gaagaactat ggttagcacg aaataaagcc ggcggtttaa aagacagttt agaatactta
     2641 cataaatttt tcttattttc ccaacaaaca aaaaaacatt tgcaaagagg tggagcgtat
     2701 ggcgcgtaaa atcaagattc aaccaaaagt tgtgaaaaag gcgattcttt tactgtttat
     2761 cgtcattttc attact
//
LOCUS       DAOSGN010000077         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_34822_length_2775_cov_2.652574, whole genome shotgun sequence.
ACCESSION   DAOSGN010000077 DAOSGN010000000
VERSION     DAOSGN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34822_length_2775_cov_2.652574"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK087_01655"
     CDS             complement(<1..637)
                     /locus_tag="PK087_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOH18008.1"
                     /translation="MKRIINYLILFVLVPVVLFLGYFLYQDSQFEIISIIIALLACVP
                     FFIHFEKKRPDARELVLVAIFIAISVVSRILFAPIPGFKPVAAFTIIVAMVYGKEMGF
                     MVGALSAVLSNMYFGHGPWTPFQMFAWGIIGFFAGLFFSGKPTKRLWWVVLYGALSGV
                     VFSLILDIWTTLSVDDGFVFARYLGFVAASFPMMIIYVISNCLFLLILEKPL"
     gene            complement(634..2256)
                     /locus_tag="PK087_01660"
     CDS             complement(634..2256)
                     /locus_tag="PK087_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH18009.1"
                     /translation="MALLDVKNVSFTFANQVKALKDVSFQIEEGEFVLLTGSTGSGKT
                     TLLRLIKQELAPYGKMTGTITYQNRDLLSLSPRERASEIGYVFQNAESQIVSDFVYQE
                     LAFGLENLGYSSDVIRLRVGEMASFFGINSWFRHRTAELSGGQKQMLNLASIMVMEPK
                     ILLLDEPTSQLDPIATTDFLQTLKRLNSELGLTIIIVEHHLEEVWTLVDRVLVLDQGE
                     LVADNPPRIISKQTTIPGLAFPSPVKIYREFSFTDECPQTINEAKKWLSHHFHNHVKK
                     MTLNENCEEKPALVNLENIWFRYEKKANDILRNLHLDVRKGEILTIVGGNGSGKSTLL
                     KILAGLIKPYLGKVRFLGKTPPSISFLPQNPQILFVADTVMAELKDMEPYLALSKADF
                     SLRLQQNIALFSLQNILDQHPYDLSGGEQQKLALAKILLKNPDLLLLDEPSKSLDASF
                     KKNLMNILNDLHQSGKTIVIVSHDIEFAARVSHRNAMLFDGTIVADGHPHSFFSGNTF
                     YTTNANRLSRHMYESLITEEEVIMMAQLNGAK"
     gene            complement(2229..>2775)
                     /locus_tag="PK087_01665"
     CDS             complement(2229..>2775)
                     /locus_tag="PK087_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOH18010.1"
                     /translation="VFNEVMTADKFYYLFGRVAPTITLVLTMGLRFIPLMVKQMRNIE
                     EGQKGLGLLTQTSFLKKLKHYLRMFSILLTWSLEHALETADAMKSRGYGLKPRTSFSS
                     FSFHRQDLGSLIYLLLFFSIVVSGEFLNIYASSFFPNLSNFQFGIRELIGYLSFSLLG
                     IFPLILVIKEALIWRYWMLKM"
BASE COUNT          973 a          548 c          503 g          751 t
ORIGIN      
        1 ttagaggttt ttcgagaatt aataagaata agcaattaga aataacataa attatcatca
       61 tgggaaatga tgccgccaca aaaccaagat aacgagcaaa aacgaagccg tcatccacgg
      121 ataatgtcgt ccaaatatcc aaaatgaggg aaaaaacaac gccgcttaaa gccccataaa
      181 ggacaaccca ccaaaggcgt ttagtgggct tgccactaaa aaataacccg gcaaaaaacc
      241 cgattattcc ccaagcaaac atttgaaatg gtgtccatgg accgtggcca aaatacatgt
      301 ttgataacac agcacttaaa gcaccgacca taaagcccat ttctttccca tataccatgg
      361 caacaataat cgtaaatgcc gctaccggtt taaacccggg aatgggagca aacaaaatcc
      421 gactgacaac ggaaatagca ataaaaatgg ccaccaacac taattcgcgg gcatcgggtc
      481 gttttttttc aaaatgaata aagaaaggaa cgcaggccaa taaggcaata ataatcgaaa
      541 tgatttcaaa ttgcgagtcc tgatacaaaa aataccccag gaataagact accggaacta
      601 aaacaaaaag aattaagtaa ttaattattc tcttcatttg gcaccattta attgagccat
      661 catgatgact tcttcttccg taattaaact ttcatacatg tggcgtgaca aacgattggc
      721 attagtggta taaaaagtat tcccggaaaa aaatgaatgc ggatggccat ccgcaacaat
      781 ggtcccatca aagagcatgg catttcgatg gctcactcgg gcagcgaact caatatcatg
      841 actgacaatc acaattgttt ttccggattg atgtaaatca tttaaaatat tcataagatt
      901 ttttttaaac gacgcatcta aacttttaga cggttcatct agcaacaaaa gatcgggatt
      961 ttttaataaa atttttgcaa gcgctaattt ttgttgttca ccaccactta aatcgtatgg
     1021 atgttgatct aaaatatttt gtaaagaaaa aagggcaata ttttgttgta atcggagaga
     1081 aaaatcggcc ttggaaagtg ccaagtacgg ttccatatcc tttaattccg ccattacggt
     1141 atcagcaaca aataaaattt gcggattttg gggaaggaaa gagatggagg gcggagtttt
     1201 tcctaaaaat cgcacttttc ctaaataggg tttaattaaa ccagctaaaa tttttaaaag
     1261 ggtggacttc ccactaccat taccgccaac aatggttaaa atttcccctt ttctaacatc
     1321 caaatgaaga ttacgaagaa tatcgttggc ctttttttcg tacctaaacc agatattttc
     1381 taaattaacc aaagcgggtt tttcttcaca attctcatta agggtcattt tttttacatg
     1441 attgtggaaa tggtgggaca gccatttttt ggcttcattg atggtctgtg gacattcatc
     1501 ggtaaaacta aactcccgat agatttttac cggactggga aaagcaagac cgggaatagt
     1561 agtttgttta ctgataatcc gcggaggatt atcggcgaca agctcacctt gatccaaaac
     1621 caacacgcgg tcaaccaaag tccaaacttc ttccaaatga tgctccacaa tgataatagt
     1681 taaacccaat tcactattta gtctttttaa ggtttgtaaa aaatccgtgg ttgcaatcgg
     1741 atccaattga ctggtcggct catcaagcaa taatatttta ggttccatga ccataatact
     1801 ggcaaggttc aacatttgct tttgcccacc ggataattcg gcagtcctat ggcggaacca
     1861 cgaattaatc ccaaaaaaac tggccatttc accgacacgc aaacgaataa catcggagct
     1921 atagcctaaa ttttctaaac caaaggccaa ttcttgataa acaaaatcgg aaacaatttg
     1981 gctttccgcg ttttggaaaa cataaccgat ttctgaggcc cgctctcgtg gggaaagaga
     2041 caaaagatca cgattttgat aggtaattgt tcctgtcatt tttccatagg gagctagttc
     2101 ttgtttaatc aagcgcaata atgtcgtttt cccactaccg gtggaaccgg tcaataaaac
     2161 aaattcgcct tcttctattt gaaaagacac atctttaagg gcctttactt gattagcaaa
     2221 agtaaaactt acatttttaa catccaataa cgccatatca aggcctcctt gataactaaa
     2281 attaagggga aaatccctaa taaggaaaac gaaagatatc ctatcaactc gcgaatacca
     2341 aattggaaat ttgatagatt agggaaaaac gaagacgcat aaatatttaa gaattctccg
     2401 gaaacaacaa ttgaaaaaaa caataaaaga taaattaaac tacctaaatc ttgacgatga
     2461 aaggaaaaag aagaaaagga ggtgcggggt ttcagtccat aacctcgtga tttcatggca
     2521 tcagccgttt caagcgcatg ctctagtgac caagtgagga ggatggaaaa cattcttaaa
     2581 taatgcttaa gtttttttaa aaaggatgtt tgcgtcaata aaccgagccc tttttggcct
     2641 tcttcaatat tgcgcatttg tttgaccatt aaaggaataa agcgtaagcc catagttaaa
     2701 actaaagtaa tggttggggc tacccgacca aataaataat aaaatttatc cgccgtcata
     2761 acttcgttaa aaaca
//
LOCUS       DAOSGN010000078         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_34841_length_2774_cov_2.522986, whole genome shotgun sequence.
ACCESSION   DAOSGN010000078 DAOSGN010000000
VERSION     DAOSGN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34841_length_2774_cov_2.522986"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="gcvPB"
                     /locus_tag="PK087_01670"
     CDS             <1..929
                     /gene="gcvPB"
                     /locus_tag="PK087_01670"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009434082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOH18011.1"
                     /translation="PASASVCGMDTIVVPSTPEGTVDITALKKVLNDEVAGIMLTNPN
                     TLGIFEKDICEIAELVHANGSLLYYDGANLNALLGIARPGDMGFDIMHINLHKTFSTP
                     HGGGGPGSGPVGVTKALVHLLPNPRVIKEKGKYRWLTNEQSIGSVSGFYGNFLVVVRA
                     YTYLRTLGKENVHHVGYLSVLNANYIKESLKEYYKLPITGLCKHEVVFDGLISEKATT
                     LDVAKRLLDYGYHPPTIYFPLLFHQTIMIEPTEVESKETIDDFINTMIKIAQEAEANP
                     DLLKTAPHNTIVRRLDDVKAARNPIIKYKDIV"
     gene            926..2257
                     /gene="lpdA"
                     /locus_tag="PK087_01675"
     CDS             926..2257
                     /gene="lpdA"
                     /locus_tag="PK087_01675"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOH18012.1"
                     /translation="MRDLIIIGAGPGGYELALEAASQGLKVTLIEKDALGGTCLNHGC
                     IPTKSFYHTAKLIKDFQEAQNLGFSPLPNWDYQKTLHRKNEIVSQLVGGIKFLLEKAQ
                     VEVIHGHGKLISPHQVQVNEQIIEGAFIVIATGSSSAKLPLPGFNLPQVLSSTEMLDL
                     DVIPKKLAIVGGGVIGIEFASIFQQFGSEVEVFEALDTILPLSDREISKRLLSYLKQS
                     GIKFHLKTMVSKIEKTDHLQLFYQEKDQELTTEADVVLVAVGRKPKLDKLGLEEVGIV
                     YDRFGIKVNEHFQTNFPHIYAIGDVTGKMMLAHSATYSGFQVLKHLLHQSDRIDFSIL
                     PSCVFTFPEVAQVGLTEEEAKSLDYRVLKSFFRANGKALTINETNGFIKIIVVDEVIR
                     GVHILGPHASDLISEAVALLNQKTTVQEMKNFIHPHPTLGEIFASAIREDH"
     gene            2317..>2774
                     /locus_tag="PK087_01680"
     CDS             2317..>2774
                     /locus_tag="PK087_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formiminotransferase"
                     /protein_id="HOH18013.1"
                     /translation="MQQIIEIVPNYSEGKNADIMQKIIAPFQNNPLISLVDLEMDASY
                     HRSVVTIIGEAQTVLRQMVESAKIAASLIDMRRHSGEHPRMGAIDVCPLIPIQNITEQ
                     ECIDLSRKLAQNINEASQIPIYLYAKSASNEQRSLLPNIRQGEFEGLATK"
BASE COUNT          824 a          499 c          584 g          867 t
ORIGIN      
        1 atccggccag tgccagtgtt tgtggcatgg atacgattgt tgttccgtca accccggaag
       61 gaacggtcga tattactgct ttgaaaaagg ttttaaatga tgaagtagcc gggattatgt
      121 taactaatcc caataccttg gggatttttg aaaaagatat ttgtgagatt gcggagcttg
      181 ttcatgctaa tggtagttta ctatactatg acggtgccaa cttaaatgct ctattgggga
      241 ttgccagacc aggggacatg ggatttgata ttatgcatat taatctccat aagacttttt
      301 ccactcctca tggtggcggg ggtccgggaa gtggtccggt gggtgttacg aaagctcttg
      361 tgcacttgct tcctaatcca agggtaatta aagaaaaagg gaaatatcgt tggcttacta
      421 atgaacaatc tattggttct gttagcggtt tttatggtaa ttttttggtt gttgttcgtg
      481 cttataccta tttacgcact ttgggaaaag aaaatgtcca tcatgttggt tatttatccg
      541 ttttaaatgc caattatatc aaagaatcac tgaaagaata ctataaatta ccaatcaccg
      601 gtttatgtaa acatgaagtg gtttttgatg gtctcatcag tgaaaaagcc acgactttag
      661 atgttgctaa acgcttgctg gactatggtt accatccgcc aaccatttat tttccgctat
      721 tgttccatca aacgattatg attgaaccga cggaagtcga atctaaagaa acgattgatg
      781 atttcattaa taccatgatt aagattgcgc aagaagcaga agcgaatccg gacttattga
      841 aaacggctcc ccacaatacg attgtccgcc ggcttgatga tgtgaaggcg gcccgtaatc
      901 ccattatcaa atataaggat attgtatgag ggatttgatt attattgggg ccggaccggg
      961 aggatatgaa ttagctttag aagcggcttc ccaaggttta aaagtaactt taattgaaaa
     1021 agacgcttta ggtgggactt gtttaaacca tggttgtatt cctactaaat ccttctatca
     1081 tactgccaaa ttgattaaag attttcaaga agctcaaaac ttaggttttt cacctttacc
     1141 taattgggat tatcaaaaaa cgcttcaccg taaaaatgaa attgtttccc aactggttgg
     1201 cggtatcaag tttttgttag aaaaagccca agttgaagtt atccatggtc atggtaagtt
     1261 aatatcgcct catcaagttc aagtcaacga gcaaatcatt gaaggtgctt tcattgtgat
     1321 agcaacgggc tcatcaagtg ctaagctacc tttgccgggt tttaatctcc ctcaagtttt
     1381 atcatccacc gagatgttag acttggatgt tattccgaaa aagttagcca ttgttggtgg
     1441 tggggttatc ggcattgaat ttgcttccat ttttcaacag tttggcagtg aagttgaagt
     1501 ttttgaagct ttggatacga ttttaccttt gagcgatcga gaaatttcta aacgcttgct
     1561 tagttattta aaacaaagcg gcattaagtt tcatttaaag acaatggttt ccaagattga
     1621 aaaaacggac catttacaat tgttctatca agaaaaagac caagaattga ccacggaagc
     1681 tgatgttgtg ctggtggctg ttggtcgtaa accgaagctt gacaagcttg gactggaaga
     1741 agttggaatt gtttatgatc gctttggcat caaagtcaat gagcatttcc aaactaattt
     1801 tccccatatt tatgccattg gcgatgttac cggaaaaatg atgttagcgc acagtgcgac
     1861 ttacagcggc ttccaagtct taaagcattt gttgcatcaa agtgaccgca ttgatttttc
     1921 gattcttcct agttgtgtct ttacatttcc ggaagttgcc caagttggtt taacggaaga
     1981 agaagctaaa agccttgatt accgagtttt aaaatccttc ttccgggcta atggtaaagc
     2041 cttaaccatt aatgaaacca atggttttat taaaatcata gttgtggatg aagttattcg
     2101 aggtgtacac attttaggac cccatgccag tgatttaatt tcggaagcgg ttgccttgct
     2161 taatcaaaaa acaaccgtcc aggaaatgaa aaatttcatt catccccatc ccaccttggg
     2221 agaaattttt gcttcagcaa ttcgtgagga ccattaattt ggtcctttta tttttgccta
     2281 gttaatgtta taatatttaa aagtaggtga atcacaatgc aacaaataat tgaaattgtt
     2341 cctaattatt ccgaaggcaa aaatgccgat attatgcaaa aaatcattgc cccctttcaa
     2401 aataatccgc tcattagttt agtggatttg gaaatggatg cttcgtacca tcgcagtgtg
     2461 gtgacaatta tcggtgaagc ccaaaccgtc ttaaggcaaa tggtggaaag cgctaaaatc
     2521 gctgcgtctt tgattgatat gcgtcgtcat tccggtgaac atccgcggat gggagcaatt
     2581 gatgtatgcc ccttaatccc cattcaaaat atcactgaac aagaatgtat cgatttaagt
     2641 cggaaattag cccaaaacat caatgaagca agccaaattc cgatttattt gtatgcgaaa
     2701 tcagccagta atgagcaaag gtcattatta cccaatattc gtcaaggtga atttgaaggt
     2761 ttagctacga aaat
//
LOCUS       DAOSGN010000079         2730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_35674_length_2730_cov_2.750654, whole genome shotgun sequence.
ACCESSION   DAOSGN010000079 DAOSGN010000000
VERSION     DAOSGN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35674_length_2730_cov_2.750654"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK087_01685"
     CDS             complement(<1..217)
                     /locus_tag="PK087_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOH18014.1"
                     /translation="MITKELCQEVLQHALQTGGDFAEIFCESTYNNAYEMTSGKTTKI
                     TGNHTYGLSLRILKGFLEVNGYTNNLSR"
     gene            complement(313..1764)
                     /gene="gltX"
                     /locus_tag="PK087_01690"
     CDS             complement(313..1764)
                     /gene="gltX"
                     /locus_tag="PK087_01690"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOH18015.1"
                     /translation="MGDNLKQKVRTRFAPSPTGFMHVGNLRSALYGFLFARQNGGSFI
                     LRIEDTDLERFVPGAVDVIYSTLKQAGISFDEGPNEGGAYGPYVQSERKHLYLEYAKK
                     LLASKHAYYCFCTKERLDSLKDEMGVARYDKHCLHLSEEEVNERLARGEKFVIRQNMP
                     TQGTSSFHDLVFGNITIDNSELEDHILIKSDGMPTYNFANVVDDHLMAITHVIRGNEY
                     LSSTPKYNLLYQAFGWTIPKYMHLTPIMKDETRKLSKRHGDANFDDFINKGYLSQAIV
                     NYIALLGWSPKSTEEKFTMDELIEKFSIAGLSKSSSIFDENKMKWLNGEYIKELSFAD
                     FMKYARPFFEKSVIKDRYDYEKFGKILQSRIEIFSEIPEKVAFIDQYHFIDPIMYDNK
                     KFKIDAGVAKEVLLEAILVLDDCQLWNEEMLHAAIKTISEKMQIKSGAVYSVLRIALS
                     SVAVTPGGSVEIADILGKKESMRRLRQSLLWLN"
     gene            complement(1796..>2730)
                     /locus_tag="PK087_01695"
     CDS             complement(1796..>2730)
                     /locus_tag="PK087_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18016.1"
                     /translation="GDMEATVGKLMAFVIMIPFVYHAFKTLMNTSISFKMIEKERQAL
                     DLILTLRSEMKSEPITQIEEVLSLKFQSVSYDGEGKDQSLNRVSFEIKRGEKLGILSY
                     EAESRQIIFELLTKLIRPKNGVIAINNCDINKMNTQYLRELVTSVSSEQYVNEDSIAN
                     NITFPLPFDEYKYNDALNRTGLKEIIAGLELKDQSIVNEAAAFSKAFIQRLQLANAFY
                     KDSKIFVLKNATTYLDVRSEEAIMKEVNKLKNKMVIIMSDKVYNVLGCDKILILMGGQ
                     VVEYGKLPELLQNKDSEFNKMIKKVKTTKTIRAS"
BASE COUNT          836 a          566 c          445 g          883 t
ORIGIN      
        1 cacgacttaa attattggta taaccattta cttctaaaaa gcctttgaga atccttaatg
       61 ataagccata agtgtgattc ccggttattt tagtcgtctt tccgcttgtc atttcatagg
      121 cgttattata agtcgattcg caaaaaatct ccgcaaaatc gccgcccgtt tgtaaagcgt
      181 gttgtaaaac ttcttgacaa agttccttag taatcattaa catcacatcc tttataatta
      241 aagattatac taaaaaaacc aagtaatatc aagtaaaaca atgaaagtaa aaaagcatcg
      301 cgatgatgct ttttagttaa gccacaataa actttgacgc aaacgacgca tggattcttt
      361 ttttcctaaa atatcggcga tttccactga tcctcccggg gtcacagcga cactgcttaa
      421 ggcaattcgt aaaacggagt acacagcccc acttttaatt tgcatctttt cgcttattgt
      481 cttgatggcg gcatgtagca tttcctcatt ccacaactga caatcgtcta aaacaagaat
      541 ggcttccaaa agaacttctt tcgcaacacc agcatcgatt ttaaattttt tattgtcata
      601 catgatgggg tcaatgaaat gatattggtc aatgaaagcc actttttcgg gaatttcact
      661 aaagatttca atccggcttt gcaatatctt cccaaacttt tcataatcat agcggtcctt
      721 aatcaccgat ttctcaaaaa acggtcgggc atacttcata aaatccgcaa aactaagctc
      781 tttgatatat tcgccattca accatttcat tttattctca tcaaaaatac tgctggattt
      841 ggataaaccg gcaatgctga atttttcaat caattcgtcc atcgtgaatt tctcttccgt
      901 ggatttagga ctccaaccta gcaaagcgat ataattaaca atggcctgac ttaaatagcc
      961 tttgttgata aaatcatcaa agttggcatc gccatgacgc ttgcttaatt tgcgggtttc
     1021 atctttcata attggtgtta agtgcatgta cttgggaatc gtccacccaa aggcttgata
     1081 aagcaaatta tatttaggcg tactgcttaa atactcattg ccccgtatca catgagtaat
     1141 cgccataagg tgatcatcga cgacattagc aaaattataa gtcggcatcc cgtccgattt
     1201 gattaaaata tgatcctcca gttcgctatt gtcaatggta atattgccaa aaactaaatc
     1261 atgaaaggaa ctggttcctt gagtcggcat attttgacga atgacaaatt tttcaccgcg
     1321 agccaagcgc tcattaacct cttcttctga taaatgcaaa caatgtttat cataacgagc
     1381 cacacccatc tcatctttta aactgtccaa acgttctttt gtacaaaaac aatagtaggc
     1441 atgttttgat gccaatagtt tcttcgcata ttctaaataa agatgtttgc gttcggactg
     1501 cacatagggg ccataagccc ccccttcatt tggaccttca tcgaagctaa tccccgcttg
     1561 ttttaaagtg ctgtaaatca catccaccgc tccgggaaca aagcgctcta aatcagtgtc
     1621 ctcaatgcga agaataaaac tccctccatt ttggcgggcg aacaaaaagc catagagagc
     1681 ggaacgtaaa tttcccacat gcataaagcc tgttggactg ggggcaaaac gagtacgaac
     1741 tttttgtttt aaattatctc ccataatctt tccttttttc ttaataaaaa agcgtctaag
     1801 acgctcttat cgttttagtg gttttcacct ttttaatcat tttattaaat tcggaatctt
     1861 tgttttgcaa taactccggt aatttaccgt attccacaac ttgaccaccc atgagaatca
     1921 ggattttgtc gcaacctaaa acattgtaaa ctttatccga cataataatg accatcttgt
     1981 tttttaattt gttaacttct ttcatgatgg cttcttcact gcgaacatct aaataagtgg
     2041 tcgcattttt taaaacaaat attttagaat ccttataaaa ggcattagcc aattgcaatc
     2101 tttgaatgaa ggctttggaa aaagcggctg cttcattaac aatgctttga tcctttaact
     2161 ctagcccagc aatgatttct tttaaaccgg tgcggttaag cgcatcattg tatttatatt
     2221 catcaaaagg cagcgggaaa gtaatattat tagcaatgga atcctcattg acatattgtt
     2281 cggaagaaac ggatgtcact aattcccgta aatattgggt attcatttta ttgatatcgc
     2341 aattattaat ggcaatcaca ccatttttag ggcggattaa tttggtcaag agttcaaaaa
     2401 tgatttggcg gctttcagct tcataggaga gaatccctag tttttcaccg cgtttaatct
     2461 caaaagagac tcgattaagg ctttggtctt ttccttcacc atcataacta acactttgaa
     2521 attttaaaga cagtacttct tcaatttgag taattggttc cgatttcatt tcgcttcgaa
     2581 gagtcaaaat caaatcaagg gcttgtcttt ctttttcaat catcttaaaa ctaatgctcg
     2641 tgttcattaa ggttttgaaa gcatgataaa caaagggaat catgatgaca aaggccatta
     2701 atttaccaac agtagcttcc atatctcctt
//
LOCUS       DAOSGN010000080         2719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_35870_length_2719_cov_3.211712, whole genome shotgun sequence.
ACCESSION   DAOSGN010000080 DAOSGN010000000
VERSION     DAOSGN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35870_length_2719_cov_3.211712"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PK087_01700"
     CDS             <1..545
                     /locus_tag="PK087_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH18017.1"
                     /translation="IPFDTTNVLFICGGSFEGITEIVKSRLGKKAIGFNSASVTPKVD
                     KDMYRDVTSEDIQKYGIIPELVGRLPIIAYLDILEVEDLIKILKEPKNAIIKQYQKML
                     KMDGVSLEFEDGALWEIARRAHKQKTGARGLRAIIENIMLDIMYKIPEEKDLTHCIIT
                     KEFIVSGGDAKFVKKQSKLA"
     gene            556..1686
                     /locus_tag="PK087_01705"
     CDS             556..1686
                     /locus_tag="PK087_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18018.1"
                     /translation="MKIKHDIISPAYHQVYFTFSPAEMQNAYRLVRDMYSLEKPKSKK
                     AQKQLDEMVVEHLEGLMDEEIANLDVIAVSWKQFRYLCETSPDQGLVVICQFCVLPPG
                     TEIHFPPKIPTELLHVELDQMIFEDFVKQLLTANHFDYDEPQKKVTDRSLVSYDLRYM
                     QDDFLINHLENRSISFTTVNQKQFRLFLNKAIGEEVVLDNDDGVLVKAKITGITEKRY
                     PPLSDKLVAQCRFLNTKTVAELTDKVTEIYRYSTIVMVVVDYLVEFIWQTQVFVPDEF
                     VINHFYDSSKIPTKPKEREVFLQGLNKYVIKEYLLSLMDLNYRVDVQKLIKKIQEEYE
                     FDKTLFFGSKSFEDYPEYLERHLLEAKVFQYCLDKGIVELPK"
     gene            1701..>2719
                     /locus_tag="PK087_01710"
     CDS             1701..>2719
                     /locus_tag="PK087_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOH18019.1"
                     /translation="MFSNQTVVSANLPVMAIRGKVPLPNNEIRLDVARKESILALKAA
                     AESKKFIALFVQIDHSQEVPNIKNLHKTGIIAKIIYDMDSQNVHKVKMLGIVRCQIVD
                     ITETDPYWTANLITVPAINDDLDKELAYVRLLIEELQADAAKIFDGNSELANIVAKGV
                     TADKLTDVLAYNLPFDINTKLKYLNTPHVGERLRFMLEDIKREKYIRNLELDIENQVK
                     KSISESQKEYYLREKMKVIQDELGDKVRKETDVDELKQKILDAKMPPSVEEKALKELQ
                     RYSSLNNTSGESGIVRTYLDFLIALPWSKESKDTNDIKKAKEQLDSDHYGLDVVKERI
                     LEYLAV"
BASE COUNT          893 a          476 c          569 g          781 t
ORIGIN      
        1 tcatcccctt tgataccacc aatgtcttgt ttatttgcgg aggctcgttt gaaggtatta
       61 ccgagattgt aaaatcccgg cttgggaaaa aagccatcgg ctttaattcg gcttccgtca
      121 ctccgaaagt tgataaagat atgtatcgcg atgttacttc cgaagacatc caaaaatatg
      181 ggattattcc ggaattagtt ggtcgcttac cgattattgc ttatttggat attttagaag
      241 ttgaagattt aatcaagatt ttaaaagaac cgaagaatgc catcattaaa caatatcaaa
      301 agatgttaaa gatggacggt gttagcctcg aatttgaaga cggagcactg tgggaaatcg
      361 cccgccgtgc ccacaaacaa aaaaccggag ccagaggact aagagccatc attgaaaaca
      421 tcatgttgga tattatgtat aaaattcctg aggaaaaaga tttaactcat tgtattatta
      481 ccaaagaatt tattgtttcc ggtggcgatg cgaaattcgt caaaaagcaa tccaagttag
      541 catagagaga gattaatgaa aataaaacac gatattatta gccccgccta tcatcaagtt
      601 tactttacct tttcgcccgc cgagatgcaa aatgcttatc ggttggtgcg ggatatgtat
      661 tcgcttgaga aaccgaaaag taaaaaggcc caaaagcaat tggatgaaat ggtggtggaa
      721 cacttggaag ggctgatgga tgaagaaatt gctaaccttg atgttatcgc cgtcagttgg
      781 aaacaattcc gctatctttg cgagacttca cctgatcaag ggctggttgt catttgtcag
      841 ttctgtgttt taccgccggg gactgaaatc cattttccac ccaaaattcc cactgagtta
      901 ctacatgttg aacttgacca aatgattttt gaagattttg tcaagcaatt gttgaccgct
      961 aaccattttg attatgatga accccagaaa aaagtcactg accgtagttt agtaagctat
     1021 gatttacgct atatgcagga tgatttctta atcaaccatt tagaaaaccg cagcatcagt
     1081 ttcacaactg ttaaccaaaa acaattccgc ttattcctaa acaaagcgat tggtgaggaa
     1141 gtggttttgg ataacgatga cggggtgctt gtgaaagcca aaataacggg gattactgaa
     1201 aaaagatatc cccccttaag cgataaatta gttgctcaat gccgtttttt gaataccaaa
     1261 acggttgcgg aattaacgga taaagtcacg gaaatctacc gttattccac tatcgtaatg
     1321 gtggtggtcg attatttggt ggaatttatt tggcaaaccc aagtgtttgt tcccgatgaa
     1381 tttgtgatta atcattttta tgatagtagt aaaataccga ccaagcccaa agaacgggag
     1441 gtttttcttc aaggattaaa taaatatgtt attaaagaat atttactttc cttgatggat
     1501 ttaaattatc gtgtcgatgt tcaaaagtta attaaaaaaa ttcaagaaga atacgagttt
     1561 gataaaaccc tcttttttgg gtctaaaagc tttgaagatt atccagaata tttagaaaga
     1621 catttattgg aagctaaggt ttttcaatat tgtcttgata aaggaattgt cgaattaccg
     1681 aagtaaagga ggtgaacccg atgttttcca accaaactgt tgtttccgca aaccttcccg
     1741 tgatggccat tagaggcaaa gtgccgcttc ccaacaacga aatccgcctt gatgtggctc
     1801 gtaaagagag cattttagct ttaaaagcgg cggctgaaag caaaaaattt attgctttgt
     1861 ttgtgcagat tgaccatagt caggaagttc ccaatattaa aaatcttcat aaaaccggga
     1921 ttattgcgaa aatcatttat gatatggata gccaaaatgt ccataaagtt aaaatgctgg
     1981 ggattgtccg ctgtcaaatc gtggacatta cggaaactga tccttattgg acggccaact
     2041 taattacggt tccggccatc aatgatgact tggataaaga attggcttat gtccgtttgt
     2101 tgattgaaga attgcaggct gatgccgcca aaatttttga tggcaattcc gaattggcca
     2161 atatcgttgc gaagggcgta acggcggaca aattgaccga tgttttagct tacaatttgc
     2221 cctttgatat caacactaaa ttgaaatatt taaatacccc gcatgtcggc gaacgtttac
     2281 gttttatgtt agaagacatc aaacgggaaa agtacatccg taacctcgaa ttggatattg
     2341 aaaatcaagt taaaaaatcc atcagcgaaa gccaaaaaga atattattta cgcgaaaaaa
     2401 tgaaagtcat tcaggatgaa ctgggtgata aagtccgcaa agaaacggat gtcgatgaat
     2461 taaaacaaaa aatcttggat gctaaaatgc caccgagcgt tgaagaaaaa gccttaaaag
     2521 aattacagcg ttacagcagc ttgaataata cttccggtga aagcggaatt gtccgcacct
     2581 atttagactt tttgattgct cttccttgga gcaaagaaag caaagatacc aacgatatca
     2641 aaaaagctaa ggagcaattg gatagcgatc attacggttt agatgtggtg aaggaacgga
     2701 ttttagaata tttggcggt
//
LOCUS       DAOSGN010000081         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_36135_length_2705_cov_3.447925, whole genome shotgun sequence.
ACCESSION   DAOSGN010000081 DAOSGN010000000
VERSION     DAOSGN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36135_length_2705_cov_3.447925"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PK087_01715"
     CDS             <1..241
                     /locus_tag="PK087_01715"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOH18020.1"
                     /translation="TYYQNWLVKAVELQTEMAQSGVYKTYLVKHESLNEAGTVTKSSY
                     ANGMEIVFNYSLSPYTYGFQTILAQNYQVVKGGQA"
     gene            238..1443
                     /locus_tag="PK087_01720"
     CDS             238..1443
                     /locus_tag="PK087_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH18021.1"
                     /translation="MSKRIKTPIYLSGPQSEQLQKKLLLKQQKIQSQLDDVTDRYVSS
                     VKKRQENPKLRMAPVFALSRKMANLELQLQRVNLGLLRLSEGKYPKRNWWEEIKFRFS
                     QLSYQKQKVIFGLLFLLPWFIGFCVFFAYPLGTSLWWSLNKMTVVSGGGYSFEFVGFG
                     NYRDLFVSETLAGATISEVLTSSIINILINLPTILIFSLFIAVLLNTKFKGHQLVKAI
                     FFIPVVYNMTVINNTLTGTFGRLFDSDLDQSLALSTQFSNFLMQVGVGSGLVTFLTDA
                     VGRIFTIVNMSGIQILIFIAALQSIPVHLYEAAKVEGATKYEMFWKITIPMISPMVLT
                     AAVYTIVDSFATSEIIRFMTVNSQGTTMSVNQPGLYSAISVIYFLANALIIALMFLAL
                     RKQVFYYDK"
     gene            1433..2458
                     /locus_tag="PK087_01725"
     CDS             1433..2458
                     /locus_tag="PK087_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH18022.1"
                     /translation="MTSNVKKTWKERTILKLKNWQMQVKEQLQSATKRQVLKNRLIDF
                     IITVFKYILVIGLCFVILYPLLLQLAIAFRAPTDVNNPTVLWIPGEFSLKNFEVSLIA
                     LRYWKALKNSFILAFGVTILQLVSTSLAGYAFARLPFKGSKLLFGLALFTIIVPQTVI
                     SLPILRSVTKMGLIGKPITIFLMAGLGMGIKSGIFIYLFRQFYRGIPTELEEAAYVDG
                     ANVFQVFYRIMLPNARGAIITVALLAFVWQWNDYYFTTLFISRSNIDFMTLTTQQVSV
                     LYGLQQALTDAGIWGLMGQEITNNPLFSSMILNTSGILVMIPLLVMYFFVQKLFVTGI
                     ERSGIVG"
     gene            2474..>2705
                     /locus_tag="PK087_01730"
     CDS             2474..>2705
                     /locus_tag="PK087_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18023.1"
                     /translation="MKTKNTLFMILILCLGLVLTACKKPDPVVDDPTASISVDVSSVS
                     VKKGAAHQIVATVANTKEALTVAYASSNTAVAT"
BASE COUNT          760 a          518 c          548 g          879 t
ORIGIN      
        1 cacatattac caaaattggc tggtgaaggc cgtcgagttg caaacggaaa tggcgcaatc
       61 gggtgtctac aaaacttatt tagtgaagca tgaaagctta aatgaagccg ggaccgttac
      121 caaatcaagc tatgccaacg gcatggagat tgtctttaat tatagtttga gcccttatac
      181 ctatggtttc caaacgattc tagcgcaaaa ttatcaagtt gttaaaggag gacaagcatg
      241 agcaaacgaa taaaaacgcc aatttattta agcggtcctc aaagtgaaca attacaaaaa
      301 aaattactgt taaaacaaca aaagatccaa tcccagttgg atgatgttac ggatcgctat
      361 gttagcagtg tcaaaaaacg gcaagaaaat ccgaaattac gaatggcgcc ggtttttgct
      421 ttaagccgta aaatggctaa tttagaatta caattgcaaa gagttaattt gggcttgttg
      481 cgcttgtcgg aagggaagta tcccaaacgc aattggtggg aagaaattaa gtttcgcttt
      541 tctcaacttt cttatcaaaa acaaaaagtg attttcggat tattgtttct cctcccttgg
      601 tttattggct tctgcgtctt ttttgcatat cccttaggaa catcattatg gtggagtctt
      661 aacaaaatga cggttgtcag tggtggtggt tattcgtttg aattcgtcgg cttcggtaat
      721 tatcgcgatt tatttgtgag tgaaacttta gccggagcca ccatttccga agtgttgacc
      781 tcatcaatta tcaatattct cattaactta ccgacaattt taatcttttc cttgtttatt
      841 gccgttttac tcaatactaa atttaaaggt catcaattag taaaagccat tttctttatt
      901 ccggttgttt ataatatgac agttattaat aacaccttaa caggaacttt cggcagattg
      961 ttcgattctg atttggatca atcactggct ttatcgacac aattctccaa ctttctcatg
     1021 caggtgggtg ttggtagcgg cttagtcact ttcttgaccg atgctgttgg ccgtattttc
     1081 acaatcgtca atatgtcggg cattcaaatt ttaattttta ttgcggctct tcagtccatc
     1141 cccgttcatt tatatgaagc agcaaaggtt gagggagcca caaaatacga aatgttttgg
     1201 aaaataacga ttccgatgat ttctccgatg gtcttaacgg cagctgttta tacgattgtt
     1261 gacagttttg cgacttcgga aataatccgt ttcatgacgg tcaattccca aggaacgacg
     1321 atgtcggtca atcaaccggg gctttattcc gccatttccg ttatttactt tttagcgaat
     1381 gcgctcatta ttgccttgat gtttttagct ttaagaaagc aggtgttcta ttatgacaag
     1441 taatgtgaaa aaaacttgga aagaaagaac catcttaaaa ctaaaaaact ggcaaatgca
     1501 agtaaaggag caattacaaa gcgccaccaa acgccaagtt ttaaaaaatc gcctaattga
     1561 ctttattatt acggttttta agtatatact agttattggt ttatgctttg tgattcttta
     1621 tcctttgctt ttacagctag caatcgcttt tcgagcgccc acggatgtta ataatccgac
     1681 agtgctatgg attccggggg aattttccct gaaaaatttt gaagtttcgc tgattgctct
     1741 tcgttattgg aaagccttaa aaaattcgtt tattttggct ttcggcgtta ccatcttaca
     1801 attagtgtcg acttctttag ccggatatgc cttcgcaaga ctacccttca aagggagtaa
     1861 actcttattc ggtttagctt tattcaccat tattgttccc caaaccgtga tttccttacc
     1921 gattttaagg agtgttacga aaatgggctt aattggcaaa ccgattacca tctttttgat
     1981 ggccggtcta ggcatgggca tcaaatcagg gatcttcatt tatctcttcc gccaatttta
     2041 tcggggcatt ccaacggaat tagaagaagc ggcctatgtt gatggggcca atgtctttca
     2101 agtcttttat cggattatgc ttccaaatgc tcgaggggcg attattaccg ttgctttgct
     2161 ggcctttgtt tggcaatgga atgattatta tttcacaacc ctctttatct ctcgcagcaa
     2221 tatcgatttt atgacattga cgacccagca agttagtgtt ttgtatggct tgcaacaagc
     2281 cttaaccgat gccggtattt ggggtttaat gggacaggag attactaata accccttatt
     2341 ctcatcgatg attttaaaca ccagtgggat tttagttatg attcccttgt tagtaatgta
     2401 tttctttgtt caaaaattat ttgtgaccgg aattgagaga tccgggattg ttggttaaca
     2461 gaaaggaaaa aaaatgaaaa ccaaaaacac actttttatg attttaattt tatgtttggg
     2521 cttggtgctg accgcatgta aaaaacccga cccggttgtt gacgatccga cggcaagcat
     2581 cagcgttgat gtcagttccg tttcggtcaa aaaaggagcg gcccaccaaa ttgtcgctac
     2641 ggttgccaat actaaggagg ccttgacagt cgcatatgct tctagcaata ccgctgttgc
     2701 cacag
//
LOCUS       DAOSGN010000082         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_36293_length_2697_cov_3.411431, whole genome shotgun sequence.
ACCESSION   DAOSGN010000082 DAOSGN010000000
VERSION     DAOSGN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36293_length_2697_cov_3.411431"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PK087_01735"
     CDS             complement(<1..244)
                     /locus_tag="PK087_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH18024.1"
                     /translation="MNKTIITHEQKRINRQKKRQEFWKQVKKSRVSYSMVAPFLIIFF
                     TFTVLPVLMSFFLSFTSFDMLQAPKFIGLDNYINLFL"
     gene            complement(248..>2697)
                     /locus_tag="PK087_01740"
     CDS             complement(248..>2697)
                     /locus_tag="PK087_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH18025.1"
                     /translation="FMLPRIWKDAFDVATQREAGKHDQKPAQLEKPRWNQFPIRDGAG
                     FYHGQSYEFVLEAGLNSFTLTGNKEPMVLQTIHFVPVQAEKTYAQTLAEYEVNRYEKV
                     DSADFGELSYIKHQGENSFEKSTPILSPVANWSSYKVDPYVKFMTRYNTIGGTTWRVA
                     GDFVSWQVEVPKTGLYQLTFKVLQNYRQGMYSTRILSINGKVPFSECRNLQFKYDNDW
                     QNVTLGNEDGAYWFYLEAGKNVITLEATIGVYAQIVRIAEETITTLNSLYRKVVMIAG
                     VNPNEYQDYLLEERIDNLFGMINDSAANLQQCIDQIIAISGERSALISSFERTLYQMK
                     QFAKSERHIQIGLKELDDNIAALGTWVMTISEQSLAIDCFYVHGSQVKLPKAGTNFFQ
                     KLWHEMVMLFGSYGANTSLESSVKTDGPTITVWISSGRDQSQLLRQLIDESFTLQNNI
                     NVRLKLVSQAALLPATLSGNGPDVAIGVGQNIPVNWGIRNALLDLTQFADFETVQNWF
                     HPSAVLPFRFQDRVYALPDTQDFLVSFVRTDIAEELNMAVPVSWDEVIDTLPQLQRQY
                     LDYYLPNSKGALSSLLYAMVAQKGGRLYDEEGTKTLLTEPKAMEAFIDFTTFFSDYGF
                     EISANFSNRFRSGEMPMGVANFSLYNTLSVFAPEIRGHWEFRPLPGYDFDGEIRNETT
                     STVSGTVILGDTKEAKASWEFLKWWLGAEAQSGYARGMEAILGAAARYPTANLKAFEK
                     LPWSAKDYQLLTSQRTKAVGVPTFPGDYIVGRYIDNAFRSSINNNINPRDSLYEYCKK
                     INIELTRKRQEFGLNGE"
BASE COUNT          821 a          589 c          512 g          775 t
ORIGIN      
        1 ttaaaaagag attaatataa ttatccaagc caataaattt cggggcttgt aacatatcaa
       61 acgaagtaaa acttaagaaa aaactcatta aaaccggtaa aaccgtgaaa gtgaaaaaaa
      121 tgattaaaaa cggcgcgacc atcgaatagg aaacacgact ttttttaact tgtttccaaa
      181 actcttgacg ctttttttgt cgattaattc gtttttgttc gtgagtaatt atggttttgt
      241 tcatcgttta ctctccattc aaaccaaatt cttgccgttt tcgggtcagt tcaatgttta
      301 ttttcttaca gtattcatat aaactatccc gaggattaat gttattgtta atggaagaac
      361 ggaaagcatt atcgatataa cgaccaacga tataatcgcc ggggaaagtc ggaaccccga
      421 cagccttcgt tctttgagat gtcaacaatt gataatcttt agctgaccac ggtaattttt
      481 caaaagcttt taaattggca gtcggataac gcgccgccgc tccgaggatg gcttccatgc
      541 ctctagcata accgctttgg gcctcagctc ccaaccacca ctttaagaat tcccagctgg
      601 ctttagcttc tttcgtgtct cctaaaataa ccgttccact aaccgttgaa gtggtttcat
      661 tcctgatttc gccatcgaaa tcataacccg gtaagggcct aaattcccaa tgaccgcgaa
      721 tttccggcgc aaaaacggat aaagtattat ataaagagaa gttggcaacg cccatcggca
      781 tttcaccact gcggaagcga ttggagaagt ttgcactgat ttcaaaaccg taatcgctaa
      841 aaaaagtagt aaaatcaata aacgcttcca tggctttagg ttccgtaagc aaggttttag
      901 tgccttcttc atcatacaaa cggccaccct tttgcgcaac catcgcatat aataaagaac
      961 ttaaggcgcc tttggagttg ggtaaataat aatctaaata ttgccgctgt aattggggaa
     1021 gggtatcaat gacttcatcc caagaaacgg gaacggccat attcaattct tccgcaatat
     1081 cggttctcac aaacgaaacc aaaaaatcct gagtatcggg aagggcataa acacggtctt
     1141 gaaacctaaa cggtaaaacg gcactcggat ggaaccaatt ttggaccgtc tcaaagtcag
     1201 caaactgcgt taaatccagc aaagcattcc gaattcccca gttaacggga atattttgcc
     1261 caaccccgat ggccacatcc ggtccgtttc cggataaggt ggccggcagc agagccgctt
     1321 gggaaaccag ttttaaacga acattaatat tattttgtaa ggtaaatgat tcatcaatta
     1381 attgccgcaa caattgtgat tgatcccgtc cggaggaaat ccaaacggta atcgtcggtc
     1441 catccgtttt gacactgctt tccaaagagg tgttggcacc ataactgccg aacaacatca
     1501 ccatttcatg ccataatttt tggaaaaagt tagtgccggc tttcggcaac ttgacttgtg
     1561 aaccgtgaac ataaaaacaa tcaatggcta aggattgttc ggaaatcgtc atcacccaag
     1621 tccctaaagc cgcaatattg tcatcgagtt cttttaaacc gatttggata tgacgttcgc
     1681 ttttcgcaaa ctgcttcatt tggtaaagcg ttctttcaaa actggagatt aaggcgcttc
     1741 tttcgccgga aatagcaata atctgatcaa tgcactgttg taaattagca gcactgtcat
     1801 taatcatccc aaacaaatta tcaatgcgtt cttctaataa ataatcctga tattcattcg
     1861 gattgacgcc ggcaatcatc acgactttac gatataaaga attaagggtg gtaatcgttt
     1921 cttccgcaat ccggacaatc tgcgcataaa cgccgatggt tgcttccagc gtaatcacgt
     1981 ttttaccggc ttccaaatag aaccagtaag ctccgtcttc attacctaaa gtcacatttt
     2041 gccaatcgtt atcatattta aattgaagat tacgacattc cgaaaacgga acctttccat
     2101 taattgataa aatgcgggtt gaatacattc cttggcggta attttgtaag accttaaaag
     2161 ttaattgata taaaccggtt ttaggaactt caacttgcca actgacaaaa tcaccggcca
     2221 cccgccaagt ggtgccgccg atggtattgt atctggtcat aaacttaaca taaggatcga
     2281 ctttataact gctccaatta gcaactggtg aaagaatcgg cgttgatttc tcaaagctgt
     2341 tttcaccttg atgtttgata taactaagtt caccaaaatc cgcactgtca actttttcat
     2401 agcgattaac ttcatattca gccagggttt gagcataagt tttttccgct tgaacaggaa
     2461 cgaaatggat ggtttgtaag accatcggtt ctttattgcc ggttaaagta aaagaattta
     2521 aaccggcttc caaaacaaat tcataacttt gaccatgata aaaacccgct ccatcacgga
     2581 tgggaaactg attccatcgt ggtttttcta attgggcggg tttttgatcg tgtttcccgg
     2641 cttctctttg ggtcgcaaca tcaaaagcat ctttccaaat tcggggcagc ataaatg
//
LOCUS       DAOSGN010000083         2686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_36518_length_2686_cov_1.966553, whole genome shotgun sequence.
ACCESSION   DAOSGN010000083 DAOSGN010000000
VERSION     DAOSGN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36518_length_2686_cov_1.966553"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>2686
                     /locus_tag="PK087_01745"
     CDS             71..>2686
                     /locus_tag="PK087_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18026.1"
                     /translation="MKKTILQKLIMLIMVAVIITFFISAPNRDNQASGYDYSLSNYWD
                     GSSLSFSELTTTTVNSLYVPFSTISTNPSAINPAKRYVISDAKDLYDFSVAAKGANRA
                     SYLSLYYVLGKDIDFDDASTLGYLIYPIGYHNEYPFLGTFDGQGFTISNLLFEPIDSE
                     QEYDEVYEQRLYFYSLFSRIGSSGTVKNLGLINTTMIQPINWGMMHYASPLAGLNQGT
                     IDHVFVIDNRAAAGLSVDGEFYLSGMVSVNQGTFTNSFLSTRFVKSEAVINNLTVNTV
                     VNENTGTLSNVYFDDTVYFPTPPTGDLGTPLETADFQNSAYFGAGWYLNKYYSATNPQ
                     AWLNNTYPILKNIDYDENGQFLISDATELLYMRDLLAASSFFRNKTYVLTQDIDMNQV
                     SYGVYRTPNVDFSGTFTSLPITNPSTTTLYNHSTDRGAYGYYSIISLSIITGRVLGTY
                     TSYGLFGLVSGTVSNINFINATLNTLDAEEHVSKIRSNLGFVAGHLNNGTISNVHALG
                     NISLPNNLELGKMYVGGLVGYGQGTISQSSAAGTINGGIHPYDPDDEKSNQSAIGGLM
                     GYSLRTTMTESISAMDITGLSYAASNTTTLYLGGLIGYGITNHFFESINRGDILSNHQ
                     NGYIYKIYQGGAIGLHTQELLTTARVNNEGAITIVTKAELIAKVAGYGNVIGTNNFIF
                     YSLANSGVVGNVFVNGTNNLSAAVLESIKLEMAGVVITEGTNAYFQGLFNQADFVLDL
                     SVCNNFAGVILSNNNYSLVSGNPVYDNVSSTYGTTVTIVQSYNTGNISAITTGVVTSY
                     QIKLSGNSLGKNINFDQLRNEGNISVHFSHETTKLLKDAPNADGVATPYKNFKLVGLL
                     EEVSEDKLATNLYNGG"
BASE COUNT          760 a          511 c          553 g          862 t
ORIGIN      
        1 caagccaacc gtttttctga agtatggggc attagcccgg attatttcga ataactaaga
       61 aaggtgactt atgaagaaaa cgatattgca aaagttaata atgctaataa tggtggccgt
      121 cattatcacc tttttcatca gcgctccgaa ccgcgataat caagcttccg gctatgacta
      181 ttccctttct aattattggg acggaagtag cttaagtttt tcggagttaa cgaccaccac
      241 agttaacagt ttgtatgttc cttttagcac cattagtact aatcccagtg cgattaatcc
      301 cgccaaaagg tatgtgatta gcgatgcgaa agatttgtat gatttttccg ttgccgctaa
      361 aggtgccaat cgggcctcat acttaagcct ttactatgtt ttagggaaag atatcgactt
      421 tgatgatgct tcaaccttgg gttatttaat ctatcccatt ggttatcaca acgaatatcc
      481 ttttttagga acttttgacg gtcaaggttt taccatttcc aacttgttat ttgaaccgat
      541 tgattccgaa caggaatacg atgaggttta cgaacaaaga ctttatttct attcgttgtt
      601 ttctcgcatc ggtagcagtg gaacggtaaa aaacttggga ttgattaaca ctaccatgat
      661 tcaacccatt aactggggga tgatgcatta tgcttcgcct ttagccggtt taaaccaagg
      721 tacgattgac catgtttttg taattgataa tcgcgccgct gccggattaa gtgtcgatgg
      781 tgaattttat ttatccggaa tggtttctgt taatcaaggg actttcacaa actctttttt
      841 gtccaccaga tttgtgaagt cggaagctgt tatcaataat cttacggtta atacagtggt
      901 caatgaaaat accggaacct taagcaatgt ctatttcgat gatactgtgt attttccgac
      961 acccccaacg ggtgatttag ggaccccttt agaaactgct gatttccaaa attccgctta
     1021 tttcggtgct ggttggtatt taaacaagta ttactcggcc accaatccgc aagcttggct
     1081 caataatact tatccgattt taaagaatat cgattatgat gaaaatggtc aattcttaat
     1141 cagcgatgct acggaattgt tatatatgcg cgatttactg gccgcctcca gttttttccg
     1201 caacaaaacc tatgtattga cccaagatat tgacatgaat caagttagct atggtgttta
     1261 tcgaacgccg aatgtcgatt tttcgggaac ctttaccagt ttaccgatta caaatccgag
     1321 cacaacgacg ctttataatc attctactga tcggggcgct tatggatatt attccatcat
     1381 ttccttaagc attattaccg gacgggtgct ggggacatat accagttatg gtttatttgg
     1441 tttggtatcg ggaacggttt ccaatatcaa tttcattaat gcgacgctaa ataccttgga
     1501 tgcggaggag catgtttcaa aaattagaag taatttgggc tttgtggccg gccatcttaa
     1561 caatggcacc attagcaatg tccatgcatt aggaaatatt tcccttccca ataatcttga
     1621 attagggaaa atgtatgtgg gcggtttagt cggctatggt caaggcacca tttcacaatc
     1681 ctccgctgcc ggtaccatta atggcggcat ccatccttat gatcctgatg atgaaaaaag
     1741 caaccaaagt gccattggcg gcttaatggg atattctttg cgcacaacga tgacagaatc
     1801 aattagtgcg atggatatca ctggtttatc ttatgccgcc tctaatacca ctactttgta
     1861 tttaggtggt ttaatcggtt atggcattac caatcatttt tttgaatcca ttaatcgcgg
     1921 tgatatttta tccaatcacc aaaatggtta tatttataaa atttatcaag gtggagcgat
     1981 tgggcttcac actcaagagt tattaaccac tgctcgtgtt aacaacgaag gtgcgattac
     2041 gattgttacg aaagcggaac tgattgcgaa agtggccggt tacggtaatg tcatcggcac
     2101 caataacttt atcttttaca gtttggctaa ttccggtgtc gttggtaatg tttttgttaa
     2161 cggaacgaat aatttatcag cggcggtttt agaatcaatt aaattggaaa tggctggggt
     2221 ggtaattacg gaaggcacca atgcctattt tcaaggactc ttcaatcaag ccgattttgt
     2281 gctcgatttg agtgtttgca ataattttgc cggtgttatc ctatccaaca ataattattc
     2341 attagttagc gggaacccgg tttatgataa tgtcagttcg acttatggaa caacggtaac
     2401 aattgttcaa tcctataaca ccggtaatat cagtgcgatt actacgggtg tagtaacttc
     2461 ttaccaaatt aagctttccg gtaattcttt agggaaaaat attaattttg accaattgcg
     2521 gaatgaagga aatatttcgg ttcatttcag ccatgaaacg acgaaattgt taaaggatgc
     2581 tccaaatgcc gatggtgttg cgacccccta taaaaacttt aaattagtgg gtttactaga
     2641 agaagtcagt gaagacaaat tggctaccaa tttatataat ggtggc
//
LOCUS       DAOSGN010000084         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_36850_length_2669_cov_3.098317, whole genome shotgun sequence.
ACCESSION   DAOSGN010000084 DAOSGN010000000
VERSION     DAOSGN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36850_length_2669_cov_3.098317"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK087_01750"
     CDS             <1..189
                     /locus_tag="PK087_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOH18027.1"
                     /translation="AKMYSPDVANKLRIQYGGSVKVNNIEELMSMPHIDGALIGGASL
                     KAEDFLTFLKVAVKAKSE"
     gene            469..1068
                     /gene="rpsD"
                     /locus_tag="PK087_01755"
     CDS             469..1068
                     /gene="rpsD"
                     /locus_tag="PK087_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOH18028.1"
                     /translation="MARYTGPSWKISRRLKYSTLETGKELLKRPFAPGQHGQRRSKLS
                     EYGIQLQEKQRVRFTYGLNERQFRKLFNLAGKMKGKHGDNFLILLESRLDNLVYRLGL
                     ATSRAQARQLVNHGHIVVDGKKVDIPSYLCKPGQKIGVKEQARKFVIIAEALGAMVAR
                     KEFVTFDEASMTGEYVRYPERNEILPDIKENLIVEFYNR"
     gene            complement(1099..1560)
                     /locus_tag="PK087_01760"
     CDS             complement(1099..1560)
                     /locus_tag="PK087_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOH18029.1"
                     /translation="MKPTTTNLLENLNHLLLDDQSAISILANASAWLNENSTDVNWLG
                     FYLFQNNHLYLGPFQGKPACNFIPLGQGVCGVAAFKKETVIVPDTSLFPGYIACDPLT
                     KSELVIPIIVNNTLFGVLDIDSPKLKRFTKADVLFFEQACRIIAHHLAKLH"
     gene            1694..>2669
                     /locus_tag="PK087_01765"
     CDS             1694..>2669
                     /locus_tag="PK087_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOH18030.1"
                     /translation="MIYFDYGATTPIDDEVLNSYIKTQKQFFANTSSMHKLGQITKQV
                     LEQATKETLDVLGLSNHHLVYISNATEANNLAIYGIAHRYLLSEGLKWRLITTKMEHP
                     SVYEVFKALEQEGYDVIYLDVSAEGTIDLEQFRQALNKQTVLVSIMWVNNIVGSIQPI
                     KEVLNLLEQYPKATFHMDAVQGLCKVQADFDFNKVDLFTMSTHKIYGPKGVGFLAYRS
                     PIRLTKRLYGSQAQDGIKPGTLDLALAVATCKALKIYYPKTKEHYQQVQALNNYLRSG
                     LANLPMIHINSPKTAVPYILSIALPSVNAETLLHALEALDIYVSAGSAC"
BASE COUNT          876 a          479 c          561 g          753 t
ORIGIN      
        1 gccaagatgt attcgccgga tgttgccaat aaattacgga ttcaatatgg cgggagcgtc
       61 aaagtaaaca atatcgaaga attgatgtca atgcctcata ttgatggggc cttaattggc
      121 ggtgccagtt taaaagcgga agatttccta acttttttaa aggtggccgt taaagctaaa
      181 agtgaataat tatcctaaaa gatactgtga aaatagtatc ttttttatcg attaggaatt
      241 aatcgctttt gggggttaaa aagcaaaaaa tcttgactta atcggaaaaa gtggtataat
      301 atcaaaagtg caatcagcac agtgcagtaa tagcagcact tggagtaatg aaaatcatta
      361 gataacacct aaagaggggt aagtaatcag ctgctgtgat gaaaaccatc gttcatctaa
      421 actttcattg atttcaggtc ctatactaat catataagga ggaaaaagat ggcacgttac
      481 actggtccaa gctggaagat ttcccgtcgt ttgaaatatt cgacattgga aaccggcaaa
      541 gaattattaa agcgtccgtt tgctcccggg caacatggac aacgccgtag taagttatca
      601 gaatacggta ttcaattaca agaaaagcaa agagttcgtt ttacttatgg attaaacgaa
      661 agacaattta gaaaattgtt taatcttgct ggcaaaatga agggaaaaca tggtgacaat
      721 ttcttgattc tattggagtc acgcttggat aacttggttt accgtttagg tttagcaact
      781 tcccgagcgc aagctcgcca attagttaac cacggacata ttgttgtgga tggcaaaaaa
      841 gttgacattc cttcctacct ttgcaaaccc ggccaaaaaa ttggcgttaa ggaacaagct
      901 aggaagttcg taattattgc ggaagcttta ggcgcaatgg ttgcgagaaa agaatttgtg
      961 acttttgatg aagcaagcat gaccggcgaa tatgttcgct acccggaacg aaatgaaatt
     1021 ttaccggaca ttaaggaaaa tctcatcgtt gagttctaca atcgctaata aaaagatccg
     1081 tgaaaatcac ggattttttt aatggagttt cgcaagatga tgagcgataa ttcgacaggc
     1141 ttgttcaaag aataaaacat cggctttggt aaaacgcttt aacttcgggc tgtcaatgtc
     1201 taggacccca aacaaagtgt tgtttactat tatcgggatg accaattcgg atttcgttag
     1261 aggatcacag gcaatatatc ccgggaacaa ggaagtatcg ggaacgatga cggtttcctt
     1321 tttgaaggcc gccaccccac aaacaccttg acctagggga ataaaattac aggcaggttt
     1381 cccttgaaaa ggaccaaggt aaagatgatt attttgaaaa aggtaaaaac ctaaccaatt
     1441 gacatcggtg ctgttttcat ttagccaagc ggaagcattg gcaagaatgg aaattgccga
     1501 ttggtcgtca agtaataaat gattgaggtt ttctaaaaga ttggtagtag tcggtttcat
     1561 aggaatgctc cttctaataa tgatatttta ccaaaagtac aagtaaaaca ccatatcttc
     1621 ataatattat tttccaaaag ctcataaata aggtataata accctaggta gcaagaaagc
     1681 caaggagggg caaatgattt attttgatta tggggcgacg actcccattg atgatgaagt
     1741 tttaaatagc tatataaaaa cccaaaaaca gttttttgca aacacttctt ccatgcacaa
     1801 attagggcaa atcactaagc aagtgcttga acaggccacg aaagaaacgc ttgatgtttt
     1861 gggattaagc aatcatcatt tagtttatat cagcaatgcg acggaagcga ataatttagc
     1921 gatttatggt attgctcatc gctacttgtt aagtgaaggc cttaagtggc gattaataac
     1981 aacgaaaatg gaacacccgt ccgtttatga agtgtttaaa gctctcgaac aagaaggcta
     2041 tgatgtcatc tatttagatg ttagtgcgga aggaacaatt gatttagaac aattccggca
     2101 agcgctaaac aaacaaacgg tgttagtgtc gattatgtgg gttaacaata ttgttggttc
     2161 catacaaccg attaaggaag ttcttaactt attagaacaa tatccgaaag ccacatttca
     2221 catggatgcg gttcaaggtt tatgcaaagt tcaagccgat tttgatttca ataaagtcga
     2281 tttattcacg atgagcaccc ataaaatcta tggtcctaaa ggggttgggt ttttagccta
     2341 tcgttccccc atccgtttaa ctaaacgctt atatggctca caagcacaag acggtattaa
     2401 gccggggacg cttgatttgg ccttggcggt tgccacttgt aaagccttaa aaatctatta
     2461 cccaaaaacc aaggaacatt atcagcaagt ccaagcgctt aataattatt tacgaagcgg
     2521 cttagcaaat ttaccgatga ttcatatcaa tagcccgaaa acggctgttc cctacattct
     2581 aagtattgcc cttccttcgg taaatgcgga aacccttctt catgctttag aagccctaga
     2641 tatttatgtt tctgccggtt cggcttgtc
//
LOCUS       DAOSGN010000085         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_36878_length_2668_cov_3.001531, whole genome shotgun sequence.
ACCESSION   DAOSGN010000085 DAOSGN010000000
VERSION     DAOSGN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36878_length_2668_cov_3.001531"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /gene="frr"
                     /locus_tag="PK087_01770"
     CDS             complement(<1..528)
                     /gene="frr"
                     /locus_tag="PK087_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003145637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOH18031.1"
                     /translation="MPQSIFNNGEERMEKALVALKKDLSLVRTGRANPGVLNAVMVSY
                     YGVPSPLSQIAAISVPEAQLLVIKPYDRSSLKDIEKAIQLADLNLVPMNDGTVIRINF
                     PALTEQRRKELVKDIKNMAEGTKVAIRNIRRDMVEQMKKLEKDSVISEDELKRHNDQI
                     QKITDRFVEQTDVIAK"
     gene            complement(532..1260)
                     /gene="pyrH"
                     /locus_tag="PK087_01775"
     CDS             complement(532..1260)
                     /gene="pyrH"
                     /locus_tag="PK087_01775"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOH18032.1"
                     /translation="MRKRIVLKLSGEALAGDKRVGINHHKVNEIAAEIKTLFDLGNID
                     LSIVVGAGNIWRGRDAIESGMDRSSADYMGMLATILNALALQNSLERLGCDTRTMTSL
                     EIPSVAEPYIRRKALSHLEKNRIVIFGGGNGIPFFTTDTTAALRSAELGSHLILMAKN
                     GVDGVYDSNPKDNPHAKRFSQITHQEVLDKHLDVMDLTAATLCKENNIDILVFDMNVK
                     GNIARAANDFSIGTLISNNIRKGK"
     gene            complement(1316..2188)
                     /gene="tsf"
                     /locus_tag="PK087_01780"
     CDS             complement(1316..2188)
                     /gene="tsf"
                     /locus_tag="PK087_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOH18033.1"
                     /translation="MISAALVKDLREKTGAGMMDCKKALVETNGNIEEAITWLREKGI
                     LKAQKKADRIAAEGVCHFTVKDHKALVFEVNSETDFVAKNQVFLDIVSEIGEAIINSQ
                     ATNTEEALAVASAKGTVNDLLINATATIGEKISLRRVQVFEKTAADVFGTYNHMNGKI
                     VVVSVVRGNNEVVAKDVCMHIAAMNPRFLNREQVDQDYLQKEREVLTQETINEGKPAH
                     IVDRIVAGKVDKFLKTICLVDQAFVKNLDQTVGQYLQANQCEILSYVRLEVGEGLEKR
                     KDDFAAEVMAAVNN"
     gene            complement(2181..>2668)
                     /locus_tag="PK087_01785"
     CDS             complement(2181..>2668)
                     /locus_tag="PK087_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOH18034.1"
                     /translation="GLVDTNCDPDEVDYVIPANDDAIRSVKLIVGTLANAIVEAQGGT
                     PIEIDFTDEPSTEERSGRRSIYKEGDRDSRRGDGRSRPRSSRPRPSYNRPRPAAPVAP
                     EAAPVTQETAPEAALEVTPEVAPKATPEVAPEVAPEVAPEVAPVAAPAKKKVNKEIQN
                     D"
BASE COUNT          727 a          619 c          513 g          809 t
ORIGIN      
        1 tttcgcaatc acatcggttt gttcaacaaa cctatcggtt attttttgaa tctggtcatt
       61 atgacgcttc agttcatctt cactaataac actgtctttt tccaattttt tcatttgttc
      121 aaccatatcg cgacgaatat tgcgaatggc cactttagtg ccttccgcca tgtttttaat
      181 atccttaact aactccttcc gcctttgttc ggttaaagcc ggaaaattaa tgcggataac
      241 tgttccgtca ttcatgggaa cgaggtttaa atcagccaat tgaatggctt tttcgatatc
      301 tttcaaactg gagcggtcat agggtttaat cactagtaat tgggcttcgg gaaccgaaat
      361 ggccgcaatt tgagataagg gtgatgggac accataataa gaaaccatga cagcgttgag
      421 aacaccggga ttagctcggc cggttcgcac taaacttaaa tcttttttta aagccactaa
      481 ggctttttcc attctttctt cgccgttatt aaaaatactt tgaggcatat gttattttcc
      541 tttcctgata ttatttgaaa tcaatgttcc aatcgaaaaa tcgttagcag cccgagcaat
      601 attgcccttg acattcatat caaaaactaa aatatcaata ttgttttctt tacaaagagt
      661 ggctgccgtt aaatccatca catcaagatg tttatctaaa acttcttgat gggttatttg
      721 ggaaaatcgt ttcgcatggg gattgtcttt ggggttggaa tcataaaccc catcaacacc
      781 atttttagcc attaggatta aatgcgagcc taattcggca ctccttaagg cggccgtcgt
      841 atcggttgta aagaaaggga taccgttacc accaccaaaa atcacaatcc ggtttttctc
      901 caaatgggaa agggccttcc gccgaatata gggttccgct acggagggaa tttccaaaga
      961 tgtcatcgtt cgggtatcac aacctaaacg ttctaaggaa ttttgcagcg ccaaagcatt
     1021 caagatagtc gctaacatgc ccatataatc cgcactggaa cgatccattc ccgattcaat
     1081 ggcatctcgt ccgcgccaaa tgttgccggc accgaccaca atcgataaat caatattccc
     1141 taaatcaaat aaggttttaa tttccgcagc aatttcattg actttgtgat gattaatccc
     1201 aactcgctta tcaccggcca aggcctcacc actcaatttt aagacgattc tttttctcat
     1261 ttagcctcct tttgattgat aaaaaggcac tatttgctct agtgcctgat tgtttttagt
     1321 tgttgacagc ggccataacc tcagcagcaa aatcatcttt tctcttttca agtccttcgc
     1381 caacttcaag gcgaacatat gataatattt cacattgatt agcttggaga tattggccaa
     1441 ccgtttggtc aagattctta acaaaagctt gatcaaccaa acaaatggtt ttcaagaatt
     1501 tatcgacttt acccgcgaca atccgatcaa caatgtgggc cggtttgcct tcgttgatgg
     1561 tttcttgcgt taatacttca cgttcttttt ggagataatc ttgatcaact tgttcacgat
     1621 tcaaaaaacg agggttcatg gccgcaatgt gcatgcaaac atctttcgca acaacttcat
     1681 tattgccgcg aacaacactg acaactacaa ttttaccatt catatgatta taagtgccaa
     1741 agacatcggc cgccgttttt tcaaaaactt gcactcggcg taaggaaatc ttttcaccga
     1801 tggtggcggt agcattgatt aataaatcat taaccgttcc cttggcactt gcaacagcta
     1861 aagcttcttc agtattagta gcttgactgt taataatggc ttccccaatt tcactaacga
     1921 tatctaaaaa tacttggttc ttcgcaacaa agtccgtttc actgttgact tcaaaaacaa
     1981 gggctttatg gtctttaacg gtaaaatggc aaacaccttc ggccgcaatg cggtccgctt
     2041 tcttttgggc ttttaaaatg cctttttccc gtaaccaagt gatggcttct tcaatattac
     2101 cattagtttc aactaaggct tttttacaat ccatcattcc ggcgccggtt ttttctctta
     2161 aatctttaac taaagcagca ctaatcattt tgaatctcct tattcacctt ttttttagcc
     2221 ggggcagcaa caggagcaac ttcgggcgca acttcaggag caacttcggg agcaacttcg
     2281 ggagtcgctt taggagcaac ttcaggagtc acttcaagag cagcctcggg agcagtttct
     2341 tgagtaaccg gagccgcttc aggggcaacc ggagccgctg gccgcggacg attgtagctc
     2401 gggcgtggac ggctcgaccg tggacgggaa cgaccatcac cacgacggct atcacgatcg
     2461 ccttctttat agatagaacg gcgaccgcta cgctcttccg ttgatggttc atcggtaaaa
     2521 tcaatctcga ttggcgttcc gccttgagct tctacaatcg cattagccaa tgttccgacg
     2581 attagtttaa cggaacggat ggcatcatca ttagcgggga tgacataatc aacttcatcc
     2641 ggatcacaat tggtatcaac taagccaa
//
LOCUS       DAOSGN010000086         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_38093_length_2612_cov_4.040673, whole genome shotgun sequence.
ACCESSION   DAOSGN010000086 DAOSGN010000000
VERSION     DAOSGN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38093_length_2612_cov_4.040673"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..169
                     /locus_tag="PK087_01790"
     CDS             41..169
                     /locus_tag="PK087_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOH18035.1"
                     /translation="MRHSTSLTSCIECGKCVEECPQHIQIPDELKRMKREMTFLQF"
     gene            262..1107
                     /gene="rsmA"
                     /locus_tag="PK087_01795"
     CDS             262..1107
                     /gene="rsmA"
                     /locus_tag="PK087_01795"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOH18036.1"
                     /translation="MTISLNRTKAILQKHKLRPQKARGQNFLIDDNILQKTIAAAGIT
                     ACDGVIEIGPGLGALTARLLSQAKRVLAYEIDERFIDVLKQELPQQERLLLLHEDFLK
                     ADLDFAKDYFQDCAKVYVISNIPYYITTPIISKLLKSSFRFEKILLMMQKEVGLRLVA
                     RPGTKDYNAFSVFVQTKSLVRLVFDVSAHCFIPKPEVDSVLVEIIPKNEEPKENEAQF
                     FTFVQNIFANRRKTLLNNLLRTYPSTRNQLEELFRELHININIRSENLDLKQIHDLFQ
                     KMNIS"
     gene            1130..>2612
                     /gene="ispE"
                     /locus_tag="PK087_01800"
     CDS             1130..>2612
                     /gene="ispE"
                     /locus_tag="PK087_01800"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOH18037.1"
                     /translation="MVVEKGYAKINLGLEVVNKRDDGYHDLAMIMTSIELSDELYLED
                     DPFSQQVKIDCDKMSHIALEDNLIYQAAKLLKDRYNIKKGVKIRVVKRIPEQAGLAGG
                     SADAAATLRGLNELWELKLSLEELASLGLSLGSDVPFCLYNQTAKVSGRGENLEFIDD
                     CPFAYLLLVIPSYRNSTAEVFQNFVVHHRNVGKISQLEAEIKEGNINLIAKKIFNDLE
                     VGPRQSEINRTKQMLLQAGALGSSLTGSGSAVYGIFFNEKQARNAEAKLKNKTNQEII
                     LTSIRSHRKPNLTNFTWEQIPKKPQILATTEGKATAFIALGYHRIWDHYRLIATPVST
                     TNTFLIQKLNVAVCEIKYGEEDIKDDLFEQIKKIVDKTGQGLRIHINAKHQKLLGLLN
                     PDHYLAAIIKNLDAVGINSQEIFPLFPALIEAYASEQSFEFDSKTNQYQLLGEAVFGY
                     VLVASLDLKGYNSPRYTSQAAKKKPEADRLKQAIHNRNFYEMAA"
BASE COUNT          848 a          502 c          518 g          744 t
ORIGIN      
        1 taataacgaa agtgatgccc attggcagtt taataattta atccgtcatt ctacttcgct
       61 aactagttgt attgaatgtg gaaaatgtgt tgaggaatgc ccgcaacata tccaaattcc
      121 cgatgaattg aaaagaatga aacgagaaat gacttttttg cagttttaaa gaacggtttt
      181 cgttcttttt tgtttgaaaa aaaacaaatt tacttcaata aatcatcctt gtggtataat
      241 ataactatga taaggaataa aatgacgata tcacttaatc gcaccaaagc gattttacaa
      301 aaacacaagc tccgccccca aaaagcacgc ggacagaatt ttttgattga tgacaacatc
      361 ttacaaaaaa caattgcagc cgcggggatt accgcttgtg atggcgttat cgaaatcggt
      421 cccggccttg gggctttgac ggcaagattg ttatcccaag caaaacgggt tttggcttat
      481 gaaattgatg aacgctttat tgatgtttta aaacaggaac tgcctcagca agagcggtta
      541 ttactgttac atgaagattt tttgaaagcg gatttagact ttgctaagga ttattttcag
      601 gactgtgcta aagtttatgt catcagcaat atcccttatt atattacgac cccgattatt
      661 agcaaattgc ttaagtcatc attcaggttt gaaaaaatct tattgatgat gcaaaaagaa
      721 gtcggcttaa gattggttgc caggccaggt acgaaggact ataatgcttt ttctgtgttt
      781 gtacaaacta agagtttagt tcgtttagtt tttgatgtct ccgcccattg ttttatccct
      841 aagcctgaag ttgacagcgt gcttgtggag ataattccca aaaacgaaga accgaaagaa
      901 aatgaagccc agttcttcac ctttgtacaa aacattttcg ccaaccgtcg caagacgctg
      961 cttaacaatt tattaaggac ttatccatct actcgtaatc agttggaaga attgttccgt
     1021 gagcttcata tcaatatcaa tattaggagt gaaaatttag atttaaaaca aatccacgat
     1081 ctatttcaaa aaatgaacat cagttaaatt aattcgctag gaggatgcta tggttgttga
     1141 aaaaggatat gcaaaaatta atttaggcct cgaagttgtc aataaaaggg atgatggtta
     1201 ccatgattta gccatgatta tgacctcaat tgaattatct gatgagttgt atttggaaga
     1261 tgatcctttt tctcaacaag tgaaaatcga ttgcgataaa atgagccata ttgcgcttga
     1321 agacaatctc atctaccaag ccgcgaaact ccttaaagac cggtataaca tcaaaaaagg
     1381 cgtaaaaatc cgcgttgtga aacggattcc cgaacaagcc ggcttagcgg gcggcagtgc
     1441 cgatgctgca gctactttac ggggattaaa tgaattatgg gaattaaaac ttagtttgga
     1501 ggaactggca agcctgggcc tttctttggg aagtgatgtg cccttttgcc tttataatca
     1561 aacggccaaa gttagtggtc gcggtgaaaa tttagagttt attgatgact gcccctttgc
     1621 ctatttatta ttggtcatcc cttcataccg caactcaacg gccgaagttt tccaaaattt
     1681 tgttgttcat catcgtaatg ttggtaaaat ctcgcaactg gaagcagaaa taaaagaagg
     1741 caacattaat ttgattgcga aaaaaatttt taatgatttg gaagttgggc caaggcaaag
     1801 tgaaattaac cgcacgaaac aaatgctatt acaagcggga gcactaggaa gttcattaac
     1861 cgggagcggt tcggccgtct atggaatctt ttttaacgaa aaacaggccc ggaatgcgga
     1921 ggccaaatta aaaaacaaaa ccaaccaaga aatcatttta acgagcattc gttcccaccg
     1981 gaaacccaat ttaacgaact ttacttggga acaaatcccc aaaaaaccgc aaatccttgc
     2041 cacaacggaa gggaaggcga ctgcctttat tgcccttggt tatcaccgta tttgggatca
     2101 ttaccgtctt attgccaccc ccgttagcac gactaatact tttttaatcc aaaaattaaa
     2161 tgtcgcggtt tgtgaaatta agtacgggga agaggatata aaagacgatt tgtttgaaca
     2221 aatcaaaaaa atcgttgata aaaccggcca gggactacga attcatatca atgccaaaca
     2281 ccaaaaactg ttaggtttgc ttaaccccga ccattattta gccgccatca ttaaaaacct
     2341 agatgcggtt ggaatcaaca gtcaagaaat ctttccgctt ttccccgccc ttatcgaagc
     2401 atatgccagt gaacaaagtt ttgaatttga ttccaaaacc aaccaatacc aattactggg
     2461 ggaggcggtt ttcggctatg ttttagtagc ttctttagat ttaaaaggct ataattctcc
     2521 gcgttatacc tcgcaagcgg cgaagaaaaa accggaagcg gaccgcctta aacaagccat
     2581 ccacaaccgt aatttttatg aaatggctgc aa
//
LOCUS       DAOSGN010000087         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_38128_length_2611_cov_2.951878, whole genome shotgun sequence.
ACCESSION   DAOSGN010000087 DAOSGN010000000
VERSION     DAOSGN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38128_length_2611_cov_2.951878"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1555)
                     /locus_tag="PK087_01805"
     CDS             complement(23..1555)
                     /locus_tag="PK087_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOH18038.1"
                     /translation="MSILEIKDLKFSYGDKNLFNSLQMRLFPNDHLGLIGANGVGKTT
                     LMKLMAHRLDPDEGKVVWREGVTYSYLDQHLQVDSSLTIAQYLYDVYTDLFAMEEQMN
                     KYYDSLVDADPSQYDKILKSAEFLQTTLGEKGFYLIKSKIANVLNGLGISYEVSWPMM
                     QLSSGQRVKVFLAKMLLEEKDILLLDEPTNFLDSIHVDWLAKYLNSYKKAFVVISHNL
                     DFIKAIANVVVEITNKKVEIYKGDYQSYLVQKKLREDLYKTQYEAQQKYIKKTQEFID
                     KNIVRASTTKRAQSRRKALEKIDVLEKPLMQKRITFSFPFTNAYHMEALTTKKLVIGY
                     THPLLPALSLKIRFGEKIAIVGKNGVGKTTFLKTILAQIPCLAGEFWLNPLNKITYYS
                     QEILTDKTKTAVQYIQDDYPLMDDGMIRNLLSQFGIIGELAVKEMNKLSGGEQTKVRF
                     AKLSLEKSNMLILDEPTNHLDRQAKDSLFKALALYPGTVLLVTHERSFYRHLKMKEII
                     FG"
     gene            complement(1651..>2611)
                     /locus_tag="PK087_01810"
     CDS             complement(1651..>2611)
                     /locus_tag="PK087_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOH18039.1"
                     /translation="TVSILTIFTSNDIIILTFTPFICYFSKNAKINPLPYLISEFVAA
                     NTWSMMLVIGNPTNIYLASSYGINFLQYFQIMFIPTLASSLAALGILLLLFRKQLTNK
                     TLETSDYEYCLENPVLVGIILGHLVACMILLAVSSYLGWEMWLISLSFCLSALIVLLV
                     YEMLFISRQPQLIFTSLRRTPWSLVPFVVSMFAFVLVLNRDGITTSIAGWLGKMNPIM
                     GFGLTSFLAANIINNIPMTILYTDILESVLPIAQTPALYAAVIGSNIGAYLTPIGALA
                     GIMWMQILKKQEINFSFPKFIKYGLMISIPTILIALLFLMWQF"
BASE COUNT          878 a          505 c          433 g          795 t
ORIGIN      
        1 ttgcgaatat tcatattttt tcctaaccaa agataatctc tttcattttc aaatgacgat
       61 aaaaacttcg ttcgtgagta actaataaaa ccgttcccgg ataaagggct aaagctttaa
      121 acaaactgtc tttggcttgg cggtccaaat ggtttgttgg ttcatcgaga atcaacatat
      181 tggacttttc caaactcagt ttcgcaaacc gcacctttgt ttgttcacca ccggataatt
      241 tattcatttc tttgactgcc aattcaccga taatcccaaa ttggctcaac aaattacgaa
      301 tcatcccatc atccataagt ggataatcat cttgaatata ttgaactgcg gtttttgttt
      361 tatcagtaag gatttcttgt gaataataag taatcttatt taggggatta agccaaaact
      421 cacccgccaa acaaggaatt tgggctaaaa ttgtttttaa aaatgtcgtc ttaccaacac
      481 cgtttttgcc aacaatcgct attttttccc caaaacgaat ttttaaagaa agcgccggca
      541 acaaggggtg cgtgtaacca attactaatt tcttggtcgt taaagcttcc atatggtaag
      601 catttgtgaa gggaaaagaa aaagtaatcc gcttttgcat aagcggtttt tctaagacat
      661 cgattttttc taaagcttta cgacggcttt gcgctcgttt tgttgtggaa gcgcgaacga
      721 tatttttatc aataaactct tgggtttttt taatatactt ctgctgcgct tcgtactgtg
      781 ttttatataa atcttctcga agcttttttt ggactaaata agattgataa tcgcctttat
      841 aaatttccac tttcttattc gtgatttcca caaccacatt agcaatggcc ttaataaaat
      901 ccaagttatg ggaaataacc acaaacgctt ttttataact attcaaatac ttagccagcc
      961 aatcaacatg aatactatct aaaaagttag tcggttcatc aagcaacaaa atatcttttt
     1021 cttccaataa cattttcgca agaaatactt taacccgttg accactgctt aattgcatca
     1081 tcggccagga aacttcataa ctaattccta agccgtttaa gacattagcg attttcgact
     1141 tgattaaata aaaacctttt tctcccagcg tggtttgaag gaattcggca ctttttaaga
     1201 ttttatcata ttgggatgga tcggcgtcta ccaacgaatc ataatattta ttcatttgct
     1261 cttccatggc aaataaatcg gtgtaaacat catataaata ttgagcaatc gtaagggatg
     1321 aatccacttg taaatgttga tcaaggtagg aataagtaac tccttccctc cacacgactt
     1381 ttccctcatc agggtcaaga cgatgagcca ttaatttcat caaagtggtt ttgccgaccc
     1441 cattggcgcc gattaaacca agatggtcgt ttggaaacaa gcgcatttgc aaactgttga
     1501 acaagttttt atcgccataa ctaaatttta agtctttgat ttcgagaatg ctcatggcta
     1561 tctcctttgc ttgtttattc taccatagat cagtattaaa aacaaataag aacttgagca
     1621 tttcataaaa aaagcacact ttaaggtgct ttagaattgc cacattaaaa acagtaaagc
     1681 aattaaaatt gtcggaatgg aaatcatcaa gccatattta ataaacttgg gaaaactaaa
     1741 attaatctct tgctttttca aaatttgcat ccacatgatt cctgcaagag ccccaatagg
     1801 tgttaggtaa gcaccaatgt tggaaccaat taccgccgca tataaagccg gagtttgcgc
     1861 aatcggcagc accgattcta aaatatcggt atataaaatc gtcatgggga tattgttgat
     1921 aatgttggcg gccaaaaacg atgtcaatcc aaaacccata atcgggttca tcttccccaa
     1981 ccaccctgca atactagtgg taatcccatc acgattgaga accaaaacaa aagcaaacat
     2041 agatacgaca aaagggacta aactccaagg cgttctacgt aaactggtaa aaattaattg
     2101 cggttgacgc gaaataaata acatttcata aaccaagaga acgattaagg cgcttaaaca
     2161 aaaacttaag ctaatcagcc acatttccca tcccaaatat gaggaaacag ctaaaagaat
     2221 catgcaagca actaaatgcc caagaataat accaacaaga acagggtttt ctaaacaata
     2281 ttcatagtca ctcgtttcta aggttttatt ggtcagttgt tttctaaaca gcaataacaa
     2341 aatgcctaaa gcagctaacg aagaggcaag ggttggaata aacatgattt gaaaatattg
     2401 caaaaaatta atcccatagg atgaagctaa atagatattc gtcggattac cgatgactaa
     2461 catcatactc caagtgttcg ccgctacaaa ttcactgatt aaatagggga gcggatttat
     2521 tttcgcattc ttggaaaaat aacaaataaa gggcgtgaat gttaatataa taatatcatt
     2581 ggaagtaaat attgttagta ttgagaccgt t
//
LOCUS       DAOSGN010000088         2563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_39158_length_2563_cov_3.296651, whole genome shotgun sequence.
ACCESSION   DAOSGN010000088 DAOSGN010000000
VERSION     DAOSGN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39158_length_2563_cov_3.296651"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PK087_01815"
     CDS             <1..1103
                     /locus_tag="PK087_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18040.1"
                     /translation="ANAYNGISGFEKSAIQKHRRFSIIDKAQELSWRLGASGFILTHE
                     TPKILCLCRYQQQAQAIGRNLQKKYRLNNYFVTSIQSSLKHEPIQSFLLEETPIKENI
                     KHSLEQYLIQEEIKSRQEEEIPIFEEASYQEGSPFTNDDLEIIPEEQAKAKSAYRKTI
                     RTLDRSVSTDIEGVLYLHSGGSLFKQYDFVDIAHYFQKFFHGKAINIDKEGEKIKVIF
                     FTSSLPHILGIHLLDEKDPTLRGAKGFNRLINGEFSYYQLKKSGRYSKKIIQEIYEKT
                     QSSVLIFNDIYHNRPLNCFGKENVKGASSKMDNLEFAVTRRLTETKFHNQNLLGIGRD
                     PQTNTYFFNTSFLWKVPADIGKKDSIKIAISQ"
     gene            1142..1390
                     /locus_tag="PK087_01820"
     CDS             1142..1390
                     /locus_tag="PK087_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HOH18041.1"
                     /translation="MNVTGVKIYKIEGKDKLRATATITLEDAFMVHGLKIIEGENGLF
                     VVMPAVKKNGVFKDIAHPVTSEMRNTIVTAVLEEYNKQ"
     gene            1401..2432
                     /locus_tag="PK087_01825"
     CDS             1401..2432
                     /locus_tag="PK087_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOH18042.1"
                     /translation="MKVFGIVLAAGKGTRMRSEEPKCAHYIIDKTMVEYVVDSLKGAR
                     IQDIITVVGYRHEVIEEILGDRSRYALQEQQLGTAHAVMMAEPFLKDEEGLTLIAIGD
                     MPLVSKETYASLIGFHLQSGSDLTVLTTEHPTPYGYGRVVRDEADEIVEIVEEKDCDE
                     IQKNITEINSSIYVADNQKLFKYIHFIKNNNSQKEYYLTDIVKVFKEHGLSVRAHKTN
                     NYREISGVNDKVQLMIMENELIEKINRRHLQNGVTIHSPQTVVIGMDVKCRPGSVINT
                     GSIIIQNSIIEKGAIIGPYSEIKNSIIDEGARVKYSKIKDSHIKVNSRVGPYVNMIRN
                     AIVPGDYHY"
BASE COUNT          890 a          429 c          527 g          717 t
ORIGIN      
        1 ctgcaaatgc ttataatggg attagcggtt ttgaaaagag cgctattcaa aagcatcgcc
       61 gtttttccat cattgataaa gcgcaagaac ttagttggcg tttgggggcc agcggtttta
      121 tcttgaccca tgaaaccccg aaaatccttt gtttgtgccg ttatcaacaa caagcgcaag
      181 ccattggtcg taatctgcaa aaaaagtatc ggctgaacaa ttactttgtt accagcattc
      241 aaagctcttt gaaacatgag ccgattcaaa gctttttgct tgaagaaacc ccgattaaag
      301 aaaacattaa acattcgttg gaacaatatt taattcaaga agaaatcaaa agtcgccaag
      361 aggaagagat tccgattttt gaggaagcta gttatcaaga aggaagcccg tttacaaatg
      421 atgacttgga gattattccg gaagaacaag ccaaagcgaa gagtgcctac cggaaaacaa
      481 tccgcaccct cgaccgtagc gtatccaccg atattgaagg agtattatat ttacacagtg
      541 gcggaagttt gtttaaacaa tatgattttg tggacatcgc ccactatttt caaaagtttt
      601 ttcatgggaa agcgattaat attgataaag aaggagaaaa aataaaagtc attttcttta
      661 ccagttctct tccccatatt ttaggcattc atcttttgga tgaaaaagat cccacattaa
      721 ggggggccaa gggttttaat cgtttgatta atggcgagtt ttcctattat caattaaaaa
      781 aatccggacg ttattcgaaa aagattattc aggaaatcta tgaaaaaacg caaagttcgg
      841 ttttgatttt caatgatatt tatcataacc gaccacttaa ttgttttgga aaagaaaacg
      901 ttaagggtgc aagctcaaaa atggacaatt tggagtttgc tgttacaaga cgactaacgg
      961 aaaccaaatt tcacaatcaa aatttattgg gcattggcag ggatccgcaa acgaacacct
     1021 acttttttaa cacttctttt ttgtggaaag tgccggcgga cattggtaaa aaagatagta
     1081 ttaaaattgc catttctcaa taataaagag agtatttaag atagtagaaa ggaagaaaac
     1141 tatgaatgtt actggggtaa aaatttacaa aattgaagga aaagacaaat taagagccac
     1201 ggcgaccatt acgctcgaag acgcttttat ggtccacggc ttaaaaatca ttgaaggcga
     1261 aaacggcttg tttgtggtga tgccggccgt caagaaaaac ggcgttttta aagacattgc
     1321 ccatccggtc acatcggaaa tgcgaaacac cattgtaacc gctgttttag aagaatataa
     1381 taaacaataa ggaggaaata atgaaagttt ttggaattgt tttagccgcc ggcaaaggaa
     1441 caagaatgcg ctcggaagaa ccgaaatgtg cccattatat cattgataaa acaatggttg
     1501 aatatgttgt tgacagctta aaaggagcaa gaattcaaga tattattacc gttgttggct
     1561 atcgtcatga agttattgaa gaaatattag gcgatcgtag tcgttatgca ctacaagaac
     1621 aacagttagg aacagcccat gccgtgatga tggcggaacc ttttttgaag gatgaagaag
     1681 gattaacatt gattgcgatt ggtgatatgc ctttggtatc gaaagaaacc tatgcctcat
     1741 taatcggttt tcatttacaa agtggctccg acttaaccgt tttaaccacg gaacatccga
     1801 ccccttatgg ctacggaaga gttgtccgtg atgaagccga tgagattgtc gagattgtgg
     1861 aagaaaaaga ctgtgatgaa attcaaaaga acatcacgga aatcaactct tctatctatg
     1921 ttgcggataa ccaaaaattg tttaaatata ttcactttat taaaaacaat aactcgcaaa
     1981 aagagtatta tttaacggat attgtgaaag tatttaaaga acatggttta agtgtccgcg
     2041 ctcacaaaac aaataattat cgggaaatta gcggtgttaa tgataaagtt cagttgatga
     2101 taatggaaaa tgaattgatt gaaaaaatca accgccgcca cttacaaaac ggtgtcacca
     2161 ttcatagtcc ccaaaccgtc gttatcggta tggatgtgaa atgccgaccg gggtcagtca
     2221 ttaatacggg ttcgattatc attcaaaact ctattattga aaaaggtgct atcatcggtc
     2281 cttattcgga aatcaaaaac tccattattg atgaaggggc cagagttaaa tactcgaaaa
     2341 ttaaggattc gcatatcaaa gtaaactcaa gggttggacc atatgttaat atgattcgca
     2401 atgcgattgt ccccggggac tatcattatt aaaaagcgta atcgttttta ctttggtttt
     2461 cattgaaaca agcatcaaga tgatgtataa taaaattgat agcataggag gattgaatgg
     2521 cggaaataaa tggtaagaaa atcaagtttt tcacattaaa ctc
//
LOCUS       DAOSGN010000089         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_39631_length_2543_cov_2.421624, whole genome shotgun sequence.
ACCESSION   DAOSGN010000089 DAOSGN010000000
VERSION     DAOSGN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39631_length_2543_cov_2.421624"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /gene="groEL"
                     /locus_tag="PK087_01830"
     CDS             <1..1183
                     /gene="groEL"
                     /locus_tag="PK087_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOH18043.1"
                     /translation="ASVATISSSSEEIGKIIAEAMDKVGSDGVINVDESKGFDTYLEV
                     VEGLQYDKGYVSPYMVSDREKMQVTLENALVLVTDQKLSTIKDVLPVLEQIVQANKPL
                     LIIADDLENEVVSTLIVNKLRGTFNVVATKAPGFGDNQKDLLSDIATMTGATFFAKDL
                     NMELKDLTLEHLGQAKKIVVTKDNTTLIDGSGDPEAIQNRIAEIRKHMEVSTSEYDKK
                     RFSERLGKLTKGVAVVKVGAMTETELKEKKLRIEDALNATKAAIEEGIIIGGGAAYVE
                     VYKELKGKIKSDVVDIQKGINVVLDSLFTPTHQIAENSGYDAGEIVEMQKKAKPNFGF
                     DAKTGKWLDMFKAGIVDPTKVARTAILNAASISALLITTEVAVVDIKEEKETPMPQGM
                     Y"
     gene            1287..>2543
                     /locus_tag="PK087_01835"
     CDS             1287..>2543
                     /locus_tag="PK087_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18044.1"
                     /translation="MLELKNITKNYYVGPMVVEALKGISIEFRKNEFVAILGPSGCGK
                     TTLLNIIGGLDRYTSGDLLVNHRSTTDFKDHDWDSYRNHSIGFVFQNYNLISHISVLR
                     NVELALTLSGVSVSERRERALKALADVGLADQIHKRPNQLSGGQMQRVAIARALVNNP
                     DVLLADEPTGALDTETSRQIMEILKAISHERLIIMVTHNSSIAQQYATRIVKLLDGLI
                     VDDNHPYHQADQVVAKKIGKEKTSMSFLTALSLSLGNLITKKARTILTAFAGSIGIIG
                     IALVLSLSNGFQRYIDKMQADTLASYPLTISETTLDFGQIMSLNRGTDLEEFPTDAII
                     HVNAIRQNIQNALITNDINDQYIHDVIEHIDPSLYNGITYSYGVQMRIFREMPFGAET
                     YHLEVSTGNHQLPFMRWNELIESDNVN"
BASE COUNT          797 a          453 c          563 g          730 t
ORIGIN      
        1 tgccagtgtt gcgacaattt cttctagttc ggaagaaatt ggcaagatta ttgcggaagc
       61 gatggataaa gttggtagcg acggtgtcat caatgttgat gaatcgaagg gctttgatac
      121 ttatttagaa gttgtggaag gcttgcaata tgacaaaggt tatgtttcac catacatggt
      181 ttcggaccgc gaaaaaatgc aagttacttt agaaaatgcc cttgtcttag ttactgatca
      241 aaaattatca acgattaaag atgtgttacc ggtgctcgaa caaatcgttc aagccaataa
      301 accgcttttg attattgctg atgatttaga aaatgaagtt gtcagtacgc tcattgttaa
      361 taaattgcgg gggactttca atgtagttgc gaccaaagct ccgggcttcg gcgacaatca
      421 aaaagatttg ttaagtgata ttgccacgat gacgggagcg acattctttg cgaaagactt
      481 gaacatggaa ctgaaggatt taactttgga acatttaggt caagccaaga aaatcgtcgt
      541 tactaaagat aatacaacac ttattgatgg tagtggggat ccggaagcga ttcaaaaccg
      601 gattgcggag attagaaaac acatggaagt ttccacttcc gaatatgaca aaaaacgctt
      661 cagcgaacgc ttgggtaaat taacgaaggg cgtggccgtt gttaaggtcg gtgccatgac
      721 ggaaaccgaa ttaaaagaga agaaattacg cattgaagat gccttaaatg caacgaaagc
      781 ggcgattgaa gaaggtatca tcatcggtgg tggtgctgct tatgtggaag tatacaagga
      841 attgaaaggc aaaatcaaat ctgatgtcgt tgatattcaa aaaggcatca atgtcgtttt
      901 agattcctta tttacaccaa cgcaccagat tgcggaaaac tccggttatg atgcgggcga
      961 aattgtggaa atgcaaaaaa aagccaaacc gaattttggc tttgatgcca aaaccggcaa
     1021 atggcttgat atgtttaaag ccgggattgt tgatcccacc aaggtcgcca gaaccgccat
     1081 tttaaatgcg gcttcgattt cagcgctctt aattacaacg gaagtagccg ttgttgacat
     1141 taaagaagaa aaagaaaccc cgatgccaca gggcatgtat taaaaagaca cttaagcaag
     1201 ctaaaaaaag cttgcttttt tttatgtaaa acaattggtt catttgttaa tttggcgtat
     1261 aatgaaaata gtgtgaggaa tacctgatgt tagaactaaa aaacattact aaaaactatt
     1321 atgtcggacc aatggtggtt gaagctttaa aaggcattag tattgaattt cgaaaaaatg
     1381 aatttgtggc cattttaggt ccttcgggct gtggaaaaac aaccctctta aatataattg
     1441 gcggcttgga ccgttataca agcggtgatt tattggttaa tcatcgttcc accaccgact
     1501 ttaaagatca tgattgggat agttatcgca atcattccat tggttttgtg tttcaaaact
     1561 ataatttaat ctcccatatt tcggttttac gaaatgtgga attggctctg acactgtcgg
     1621 gtgtcagtgt tagtgaacgc cgggaacgag ctttaaaagc tttggcggat gttggtcttg
     1681 ccgaccaaat tcataaacga cccaatcaat tatcaggcgg acagatgcaa agagttgcga
     1741 ttgctagggc cttggttaat aatcccgatg ttttgctggc ggatgaacca accggggctt
     1801 tggatacgga aaccagtcgc caaatcatgg aaatcttaaa agcgatttct catgaacgtt
     1861 tgattattat ggtgacgcat aattccagta ttgctcagca atatgcgacg cggattgtga
     1921 aactattgga cggcttaatt gttgatgaca accatcccta tcatcaagct gatcaagttg
     1981 tggcgaagaa aatcggtaaa gaaaaaacat cgatgtcgtt tttgacggcc ctgtctttaa
     2041 gtttaggaaa tttaattacc aaaaaagcgc ggaccatctt aacagccttt gccggcagta
     2101 tcgggattat cggcattgcc ctagttcttt ctttgtctaa tggttttcaa aggtatatcg
     2161 ataaaatgca agctgatact ttagctagtt atcccttaac tattagcgaa acgacattgg
     2221 attttggcca aattatgagt ttaaatcggg gcactgattt agaggaattc cctacggatg
     2281 ccattattca tgtcaatgcc atccgccaaa acattcaaaa tgccctgatt actaatgaca
     2341 tcaatgacca atatattcac gatgtcattg aacacattga tccctctctt tataacggga
     2401 ttacttattc atatggggta caaatgcgca tttttcggga aatgcctttt ggcgccgaaa
     2461 cttatcattt ggaagtatcc acaggcaatc atcaattacc atttatgcgc tggaatgaat
     2521 taattgaaag cgacaatgtt aat
//
LOCUS       DAOSGN010000090         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_40085_length_2523_cov_3.358185, whole genome shotgun sequence.
ACCESSION   DAOSGN010000090 DAOSGN010000000
VERSION     DAOSGN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40085_length_2523_cov_3.358185"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1376)
                     /gene="glyA"
                     /locus_tag="PK087_01840"
     CDS             complement(135..1376)
                     /gene="glyA"
                     /locus_tag="PK087_01840"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOH18045.1"
                     /translation="MMSLKKSDPEIYQVIQKETRRQQNNIELIASENFVSKAVLEAQG
                     SILTNKYAEGYPNKRYYGGCVHVDVLENLAIERAKKLFDANFANVQAHSGSTANMAAY
                     RALIKPGDRVLGMALDQGGHLTHGHPLSFSGIDYQFFAYHVDRETGLIDYEEVEQLAK
                     EIKPALIVAGASAYSRIIDFKRFRSICDTVKAKLMVDMAHIAGLVAAKLHPNPVLYAD
                     VVTTTTHKTLRGPRGGLILTNDEEIAKTINKVVFPGIQGGPLMHIIAAKAVCFAEALK
                     PDFIDYQKQVIANAQALSQELQMLGYTLVAKGTDNHLVIVDVKKSLGITGKMAEEILD
                     EVAITCNKNTVPFDSEKPMITSGIRLGSPSVTTRGFKENEMRLVARLIDQALRHRKQA
                     EELEKVKAQVLALTKQYPIYQ"
     gene            complement(1461..1649)
                     /locus_tag="PK087_01845"
     CDS             complement(1461..1649)
                     /locus_tag="PK087_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18046.1"
                     /translation="MKKIGWYIMLVIGLGLLVGITLIAAFSESLDGVLKTWGFMGFGY
                     LGFILFAYAWMKLSRFKK"
     gene            complement(1653..2372)
                     /locus_tag="PK087_01850"
     CDS             complement(1653..2372)
                     /locus_tag="PK087_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOH18047.1"
                     /translation="MNTIKIRHPHTKMIAHRGLSGLERENSAAAFIAAGNRSYFGTEC
                     DLHLSKDDVFVVCHDDNLKRVAGVDCLIRDTLLQDIKQIPLYEVRSTVVKDYLRVPTL
                     LEYLEISQKYQKCCVIEIKPELTMTQTKALLTEIRNFGYLHQVIFISFRFANLEKLSQ
                     LDSSLRLQFLTSKITEEVINKCINLKISLDANYQSLTFEHVKAFQNAGLEVNVWTVDD
                     VKVARKLIRWGVDYITTNILE"
     gene            complement(2395..2466)
                     /locus_tag="PK087_01855"
     tRNA            complement(2395..2466)
                     /locus_tag="PK087_01855"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2432..2434),aa:Gln,seq:ctg)
BASE COUNT          763 a          542 c          427 g          791 t
ORIGIN      
        1 gctttcttta aatctttcat cattgttcct catcttatct ttaagtattt ttattttaac
       61 ttatttcccc aataaatgca attattatct tggcttaaag caaacaaaaa agcaatcaca
      121 aagattgccc ttctttattg gtaaatagga tattgttttg ttaatgctaa gacttgtgct
      181 ttaacttttt ccagttcttc tgcttgctta cgatgacgta aggcttggtc gattaaacgg
      241 gccaccaaac gcatttcgtt ttccttaaaa cctcgtgttg ttacgcttgg tgaacctaaa
      301 cggatgccac tggtaatcat cggtttttcc gaatcaaaag gaacggtgtt tttattgcag
      361 gtgatggcaa cttcatctaa aatttcttcc gccatcttcc cggtaatacc taaactcttt
      421 ttcacatcga caatcactaa gtgattgtcg gtgcccttag cgacaagcgt atagccgagc
      481 atttgtaatt cttggcttaa ggcctgggca ttagcaataa cttgtttttg ataatcgata
      541 aaatccggct ttaatgcttc cgcaaaacaa accgccttgg ccgcaataat atgcatgagc
      601 ggtccgccct gaatgccggg gaaaacgact ttattgatcg ttttcgcaat ctcttcatca
      661 ttagttaaaa ttaagccacc ccgtggccct cgcaaggttt tatgggttgt tgtcgtgacg
      721 acatcggcat acaagacagg attagggtgg agtttcgcgg caactaatcc ggcaatatgg
      781 gccatatcaa ccatgagctt ggccttgacg gtatcacata tgctacggaa tcgtttaaaa
      841 tcaataatcc gtgaataagc actcgccccc gccacaatta aggccggttt aatttccttg
      901 gctagttgct ctacttcttc ataatcaatc aaaccggttt ccctatccac atgataagca
      961 aaaaactgat aatcaatgcc cgaaaacgat aagggatgac catgagttaa gtgtccccct
     1021 tggtcaagcg ccatccccaa aactctatca cccggtttga ttaaagctcg gtaagccgcc
     1081 atattagcgg tgctccctga atgggcttgc acattagcaa agttcgcatc aaataatttt
     1141 ttagcccgtt cgattgctaa attttcaagg acatcgacat gaacgcagcc accataataa
     1201 cgtttgttgg gatatccttc cgcatactta tttgttagaa ttgatccttg agcttcaaga
     1261 accgctttcg aaacaaagtt ttcggaagca attaattcaa tgttgttttg ttgtcgtcgt
     1321 gtttcttttt gaattacctg gtaaatttcg gggtcgcttt ttttcaaact cataatccac
     1381 ctcaccattt ataattatac aaaagaactt agaaaaaaga aataaaaaaa cgataaaaat
     1441 tattatcatt tccatcgttc ttatttttta aatcttgaca atttcatcca ggcataggca
     1501 aacaaaataa agccaaggta accaaagccc atgaaccccc aagtttttaa aacgccatct
     1561 aaactttcgg aaaaagcggc aattaaggta atgccgacta acagtcctaa accgattact
     1621 aacatgatgt accagccaat ttttttcatt gcttactcca aaatattggt ggtgatataa
     1681 tctactcccc acctaattaa ttttctcgct actttcacat catcaaccgt ccaaacattg
     1741 acttccaaac cggcattttg aaaagctttc acatgctcga aagtcaaact ttgatagttg
     1801 gcatctaaag agatctttaa atttatacat ttattaataa cttcttccgt aattttacta
     1861 gttaaaaact gtaatcttag tgaagagtcg agttgggata atttttccaa attagcaaag
     1921 cgaaaggaaa taaaaataac ttgatgcaaa tacccaaaat tcctaatttc cgtaagtaaa
     1981 gcttttgttt gcgtcatcgt taattccggt ttaatttcaa taacacagca tttttgatat
     2041 ttttgcgata tttccaaata ttctaaaagc gtgggtaccc gcaaataatc ttttactaca
     2101 gtgctcctaa cttcataaag aggaatttgc ttgatgtcct gaagtaaagt atcacgaata
     2161 agacagtcta cacccgcaac tcgttttaag ttgtcatcgt ggcaaacaac aaaaacatca
     2221 tctttgctta agtgaagatc acactcggtg ccaaaataag aacgattgcc cgcagcaata
     2281 aaagccgccg cactgttttc tcgttccaag ccggaaagcc ctcgatgagc aatcattttc
     2341 gtatgcggat ggcggatttt aatggtattc ataatacctc tttcttttaa aaaacaggac
     2401 cagaaggatt cgaaccttcg catgattggg tcagagccaa ttgccttacc acttggcgat
     2461 ggtcctatat caatagcatt gatattatat cttttttttg tacaaaaagc aaatgtttaa
     2521 acc
//
LOCUS       DAOSGN010000091         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_40753_length_2496_cov_2.530520, whole genome shotgun sequence.
ACCESSION   DAOSGN010000091 DAOSGN010000000
VERSION     DAOSGN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40753_length_2496_cov_2.530520"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1251)
                     /gene="proS"
                     /locus_tag="PK087_01860"
     CDS             complement(13..1251)
                     /gene="proS"
                     /locus_tag="PK087_01860"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOH18048.1"
                     /translation="MKQSLLFVPTLRETPKEAEVISHRILLKGGYIKQTAAGIYSYLP
                     LAYRVIKKIEAIIREEMDAMGACELLMPALQPKDLWLESGRWQDYGKELVRLKDRHDR
                     DFCLGPTHEEVVTAIVRDYINSYKKLPLALYQIQTKFRDEFRPRFGLMRGREFIMKDL
                     YTFHSTDADLDLWYDKTRQAYQNILNRLDLHYRIVNASSGNIGGASSEEFMILCDIGE
                     DTIVYSDESDFASNTELCDLPVGALSPDGKGHILHAKGIEAGHIFKLGTKYSEPMKAF
                     FLDQNQTQQPIIMGCYGMGVSRLLMAVLEQHYRDEVALWPDTIAPFKIHIIPLDKEGT
                     KAYQLSEQLHDALSNRYEVLFDDRDERPGVKFKDADLIGANYRLVCGRKAQQGLFEVT
                     NLREKTMVEMSFEQLLEFLF"
     gene            complement(1259..>2496)
                     /locus_tag="PK087_01865"
     CDS             complement(1259..>2496)
                     /locus_tag="PK087_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOH18049.1"
                     /translation="AIKNGEPENLPVDPSAYIYQKRKGYQIAPYNRTIGSKPKWQRAM
                     VIFGGPLMNFLLALVAFFVAALLIGFPNTESSQISGLTKPTADDPGSPAYIAGLRDGD
                     TIIHLKSGTLELVVDKWNDVSSFMAQYTTQGLTTPIEVTYLRNASQYQKAVVPQLILY
                     SIGVLGEMTDEGIRIIELAEVDDTMATNADLKVDDVIVALNDVPYTNLTALYQVLIDY
                     EGNSPVASENIVRVGIIREGASQDVDIRPYSHKIMSTQKTSDGELLPIVKVAMGISPT
                     YHFDLVKSLEYSGKSTLSSFTAIIDTLDLLFNDDTVTIAALSGPVGIFSITKQIAANG
                     FPQLLNWMGLLSVNVGLLNLFPIPALDGGRLLFLGYEAITRKKTNPKVETILITVTMF
                     LLFGLMIYVTFNDILDLIK"
BASE COUNT          767 a          556 c          464 g          709 t
ORIGIN      
        1 cataatatcc tcctaaaaca aaaactctaa taattgctca aagctcattt ccaccatcgt
       61 tttttcccga agattggtga cttcaaacaa gccttgctga gctttacggc cacaaaccaa
      121 acgataatta gcaccgatta aatcggcatc tttaaatttg acgcccggcc gttcatcccg
      181 atcatcaaat aaaacttcat aacggtttga taaggcatca tgaagttgtt ccgataattg
      241 ataagctttc gttccttctt tatcgagagg aataatatgg attttaaaag gagcaatggt
      301 atccggccac aacgccactt catcgcggta atgttgttct aaaacagcca tcaataacct
      361 agaaacgccc atgccgtagc aacccataat aattggttgt tgggtttgat tttggtcaag
      421 gaagaaagct ttcatcggtt cagaatattt cgttcctaat ttaaagatat gaccggcttc
      481 aatacccttg gcatgtaaaa tatggccttt gccatccgga gacaaagcac caacgggcaa
      541 atcacaaagt tcggtgttgc tggcaaagtc gctttcatca ctataaacaa tggtatcttc
      601 gccaatatca caaagaatca taaattcttc actcgaagcg ccgccgatat taccactgga
      661 agcattaaca atgcggtaat gtaaatctaa acggttaaga atattttggt aagcttgtct
      721 ggttttatca taccataaat caagatccgc atccgtgcta tggaaagtat ataaatcctt
      781 cataataaat tcccgacccc gcattaaacc aaaacgcgga cggaattcat cgcggaattt
      841 agtctgaatt tggtaaagtg ccaaaggcag ttttttataa ctgttgatat aatcgcgaac
      901 aatggccgta acaacttctt catgggtggg ccccaaacaa aaatcacgat cgtgacgatc
      961 ctttaaacga actaattctt tcccataatc ctgccagcgt cccgattcaa gccataaatc
     1021 tttgggttgg agggccggca ttagcaattc gcaagccccc atggcatcca tttcttcacg
     1081 aataatcgct tcaatctttt taataacacg ataagccagc ggcaaataac tataaatgcc
     1141 cgccgcagtt tgtttgatat aaccaccttt aagcaaaatt ctatgactga ttacttctgc
     1201 ttcttttggg gtttctctta aggttggaac aaataataag ctttgtttca tattgcacct
     1261 actttattaa atctaagata tcgttaaagg ttacataaat cattaagcca aacaataaaa
     1321 acatagtaac cgtgattaag atggtttcga ctttgggatt ggtttttttg cgcgtaatcg
     1381 cttcataacc taagaaaagc aagcgaccgc cgtctaaagc gggaatcggg aataagttta
     1441 agagcccgac attcacactt aataagccca tccaattaag taattgggga aaaccgttgg
     1501 cggcaatttg tttggtgatg ctaaaaattc ccaccggccc ggataaagcc gcaatcgtaa
     1561 cggtgtcatc attaaataac aaatcaagag tatcaataat cgccgtaaat gatgataatg
     1621 tgcttttgcc gctatattcc aaactcttca ccaaatcaaa atgataggtc ggggaaatgc
     1681 ccattgcgac tttcacaatc ggaagcaatt cgccatcact ggttttttgc gtactcatta
     1741 ttttgtgact gtaggggcga atgtcaacat cttggctagc tccttcacga atgatgccaa
     1801 cccgaacaat gttttcgcta gccaccggac tattgccttc atagtcaatt aagacttgat
     1861 ataaagcggt taaattcgta tagggaacat cattcaaagc aacaatcaca tcatcgactt
     1921 ttaaatcagc attcgttgcc atcgtgtcat caacttccgc aagctcaata atgcgaatcc
     1981 cttcatccgt catttcccct aaaaccccga tgctatataa aatcaattga ggaacgaccg
     2041 ccttttgata ttgactagca ttccgtagat aagtaacttc aatcggagtt gttaatcctt
     2101 gcgttgtata ctgagccata aaggaggaaa catcattcca tttatcaacg acaagttcta
     2161 gggtcccgct cttaagatga attatggtgt ctccatctct taaacccgca atgtaagccg
     2221 gactgccggg atcgtctgcc gtcggtttag taagccccga tatttgggaa ctttcggtgt
     2281 tgggaaaacc aattaacaaa gcggcaacaa agaaagcgac caaagctaat aaaaagttca
     2341 tcagcggtcc accaaaaatc accattgctc gttgccattt tggtttggaa ccgatggtcc
     2401 gattataggg agcgatttga taccccttgc gtttttggta aatatatgcg ctaggatcaa
     2461 ccggtaaatt ttccggttcg ccatttttga tagcaa
//
LOCUS       DAOSGN010000092         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_41133_length_2480_cov_2.315464, whole genome shotgun sequence.
ACCESSION   DAOSGN010000092 DAOSGN010000000
VERSION     DAOSGN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41133_length_2480_cov_2.315464"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PK087_01870"
     CDS             complement(<1..986)
                     /locus_tag="PK087_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH18050.1"
                     /translation="MKQATNDYLKRNKWAYSLGGIGRDMTYTLVATFFMTYIQYAGLG
                     LSVAQFSVIGILLIVGRIWDAINDPMMGAIIENTKSRYGKFKPWILIGAVLTAIVIVI
                     MFNVRPLGNKGWNFVIFFGIIFLAWEITFTMNDISYWSMLPSLSTDPKHRDKIATLAV
                     VFAGVGAFAAHAMITFLTVGNAIAGYSFISIIIALFLVGCQLLTVLGVKEPEVKDTIP
                     QENVSLKKMIRVIAKNDQLLWLILAMLLYNLGSNILVALGYNFFFMELGYDPFKVTIF
                     IVTFGVSNLSIQALYPKIVSKMGRKKLLGLSLLILAFGYLLLFLMGFLAILPV"
     gene            1121..1501
                     /locus_tag="PK087_01875"
     CDS             1121..1501
                     /locus_tag="PK087_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOH18051.1"
                     /translation="MKAKFFICEHCGNEVVMLHHSGVTPVCCGEPMKELVAHTKEAAY
                     EKHIPVVVQKGNQVDVVVGSVIHPMVEAHFIKWIYLETSKGGQFKKLAPNQEPKASFT
                     LTDEKLVAVYEYCNLHGLWAFEVK"
     gene            complement(1553..1864)
                     /gene="trxA"
                     /locus_tag="PK087_01880"
     CDS             complement(1553..1864)
                     /gene="trxA"
                     /locus_tag="PK087_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015595187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOH18052.1"
                     /translation="MENTNKVNFESAIANNRLVLVDFYANWCGPCKMMAPIIEELNKE
                     LAGKVKVLKVDVDQENELSRKYQITAIPTLILIKDGQVVSKTMGYKPKDAILAIIKNY
                     A"
     gene            1997..>2480
                     /locus_tag="PK087_01885"
     CDS             1997..>2480
                     /locus_tag="PK087_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH18053.1"
                     /translation="MDCFNKPVSRLKTASAKWNFDDEHILSFSIADSDYPTAPAVIKA
                     LKKRCDHGIFGYTRIDDEYRAIIVRWFAKRYRYQIQADWLVPTAGIVPSIALAIETFT
                     NEGDAILIQPPVYNPFYTVIEATKRETILNKLICRNYHYEMDFADLEKHFKNGVKMMI
                     L"
BASE COUNT          787 a          451 c          481 g          761 t
ORIGIN      
        1 actggtaaaa tggcaagaaa acccattaaa aataataata agtaaccaaa agctaagatt
       61 aataaactaa ggcctaagag tttcttgcga cccattttgc tgacaatctt gggatagaga
      121 gcttggatgc ttaagttgct gaccccaaag gtgacgatga aaatggtgac cttaaaggga
      181 tcataaccta attccataaa gaagaagttg tacccgaggg caaccaaaat gttactgcct
      241 aaattataaa ggagcattgc tagaatcaac cacagaagct ggtcgttttt cgcaatcacc
      301 cgaatcattt tctttagcga gacattttct tgcgggatgg tgtctttaac ttccggttcc
      361 ttgaccccta acacggttaa cagctggcat ccgaccaaga ataaggcgat gatgatggaa
      421 ataaaagaat atccggctat ggcgttgccg acggtaagaa aggtaatcat cgcatgggct
      481 gcaaaggctc ccacccccgc aaagacaacc gctaaagttg cgattttatc gcgatgtttg
      541 gggtctgttg atagactagg gagcattgac caataagaga tatcattcat ggtaaaggtg
      601 atttcccaag ctaaaaatat aataccaaag aaaataacaa aattccaacc cttatttcct
      661 agggggcgaa cattaaacat gataacaatg acaatggccg ttaataccgc cccaattaaa
      721 atccatggtt taaatttgcc ataccgtgat ttggtatttt caatgatggc acccatcatt
      781 ggatcattga tggcatccca aattctaccg acaatgagca aaatcccaat aacggagaat
      841 tgggcgacgg ataaacctaa tccggcatat tgaatgtagg tcataaaaaa ggtggctact
      901 aatgtataag tcatatcgcg gccaataccg cctaaactgt aagcccactt gtttcgtttc
      961 aagtaatcgt ttgttgcttg cttcattttt tcctccatga tttaattaca aggctattat
     1021 accatttttt taggaaatga aaaacaagtt ttaacataag tatattgatt tacaagcaat
     1081 aatgactatg ttataatatc catgatagga ggttgatatt atgaaagcaa aattctttat
     1141 ttgtgaacat tgtggaaacg aagttgtgat gcttcatcat tccggtgtta cccctgtttg
     1201 ttgcggtgaa cctatgaagg aactagttgc gcacaccaag gaagcggctt atgaaaaaca
     1261 tattcccgtt gtggttcaaa agggcaatca agttgatgtt gtggtcggaa gcgtaattca
     1321 cccgatggtg gaagcgcatt tcattaaatg gatttatttg gaaacaagca aaggcggaca
     1381 attcaaaaaa ttggcaccaa accaagaacc aaaagctagt tttaccttaa cggatgaaaa
     1441 attggtcgcc gtttatgagt attgcaatct gcatggtttg tgggcttttg aagttaagta
     1501 acaaaaaaag tgattcatat catatggatc acttttttaa ttcatagagt ttttaagcat
     1561 agtttttaat aatagcaaga atggcatcct tcggtttata tcccattgtt ttggaaacaa
     1621 cttggccatc tttaattaaa atgagggttg gaatggcggt aatttgatat ttccttgata
     1681 attcattttc ttgatcgaca tcgaccttta aaacttttac ttttccggct aattctttgt
     1741 ttagttcttc aataatggga gccatcatct tacatggacc gcaccaattg gcataaaaat
     1801 caactaaaac taaacggtta ttagcaatgg cggattcgaa attgactttg ttcgtatttt
     1861 ccatcatatt ctcctttcct ttttattcat tatacaatgt ttttaaataa tttactagtt
     1921 atttgctttt atttgtggtt ggaaattacg aataataatg ttataataaa acaaatggaa
     1981 atgttaaagg agaattatgg attgttttaa taaacctgtt tctcgattaa aaacggcttc
     2041 ggccaaatgg aactttgacg acgaacacat cttgtccttt tcgattgctg attccgatta
     2101 tccaaccgca ccggcagtta ttaaagcttt gaaaaagcgc tgcgaccatg ggatttttgg
     2161 ttatacaaga attgatgatg aataccgtgc cattatcgtt cggtggtttg ccaagcgcta
     2221 ccgttatcaa attcaagctg attggcttgt gcccacggcg ggcattgttc cttccatcgc
     2281 cttagcgatt gaaaccttca ccaatgaagg tgatgccatt cttatccagc ccccggtcta
     2341 taatcctttt tatacggtga ttgaagcaac taaaagggaa acaatcttaa ataaattaat
     2401 ctgtcgcaac taccattatg aaatggattt tgctgatttg gaaaagcatt ttaaaaatgg
     2461 tgtcaaaatg atgattcttt
//
LOCUS       DAOSGN010000093         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_41273_length_2475_cov_2.932645, whole genome shotgun sequence.
ACCESSION   DAOSGN010000093 DAOSGN010000000
VERSION     DAOSGN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41273_length_2475_cov_2.932645"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /gene="uvrB"
                     /locus_tag="PK087_01890"
     CDS             <1..1944
                     /gene="uvrB"
                     /locus_tag="PK087_01890"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOH18054.1"
                     /translation="TGDQPEAIQQLIDNLQKGVKEQTLLGATGTGKTFTIANVIAKIG
                     KKTLVLAHNKTLAAQLYSELKTFFPKNRVEYFISYYDYYQPEAYVAKKDLYIEKDADI
                     NEDIDQMRHSAVSSLMERDDVIVVASVSCIYGIGDPEDYQDKILTLRQGEVIKREQLM
                     ERLVNMQYERNDYALKRGSFRIKGESLDIIPPYEKDQAIRLEFFDNEIEKIKIVDTVT
                     GRALVEKTMTNIFPATLFAIGEAKLKEAITRIKAELAERILFFESQKKPLEAERIKQR
                     TEYDLEMLEEVGSCSGVENYSRHLSLRAEGETPYTLLDFFKKDFLLVVDESHVTLPQV
                     RGMYHGDRSRKQSLVDYGFRLPSALDNRPLTFSEFYSKLDRIIYVSATPSDFEKKRSH
                     SIVEQIIRPTGLLDPLVEVRPIEGQIDDLIKEINQRISANERILILTLTIKMSEDLSK
                     YLKELDYKVAYLHSEIKSLERIEIIRALRQGKYDILVGINLLREGLDIPEVSLIAILD
                     ADKEGFLRSETSLIQTIGRAARNANGKVILYASQMTGSMERAIEETYRRRSIQDEFNQ
                     KHHIVPKTIYKHISDNIVITREIKDEKSFVDMSSLKDMSTLEKRHLIMRLETQMKEAA
                     KKLDFEQAMALRDIIFELKADLR"
     gene            1950..>2475
                     /locus_tag="PK087_01895"
     CDS             1950..>2475
                     /locus_tag="PK087_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOH18055.1"
                     /translation="MKKKVIVGLSGGVDSAVACINLLEQGYDVTGIFMRNWDSSLNND
                     ILGNPHLLDDVCPQEKDYLDAMEVANKLGIPFLRIDFVQEYWESVFQHFLNEYRLNRT
                     PNPDIICNKEIKFKAFLNKAVLLGADYIAMGHYARTVFEPEVKLLRGLDAMKDQSYFL
                     CQLSKEQLKKTLFPI"
BASE COUNT          792 a          427 c          536 g          720 t
ORIGIN      
        1 accggtgatc aaccggaagc cattcaacaa ttaatcgata atttacaaaa gggcgttaaa
       61 gaacaaaccc ttttaggcgc caccggcacg ggaaagactt ttaccattgc caatgtgatt
      121 gcgaagattg gtaaaaaaac cttggttttg gcgcacaata aaactttggc agcccaatta
      181 tatagcgaat taaagacctt ttttcctaaa aatcgggtgg aatactttat ctcttattat
      241 gattattatc aaccagaagc ctatgttgcg aaaaaggatt tgtatattga aaaagatgcc
      301 gatatcaacg aagatatcga ccaaatgcgg catagcgcgg tcagttcttt aatggaacgc
      361 gatgatgtta ttgttgtggc cagtgtttct tgcatttatg gaattggtga cccggaagat
      421 tatcaagata aaatattaac acttcgccaa ggagaagtga ttaaacggga acagttgatg
      481 gaacgtttag tcaatatgca gtacgaacgg aatgactatg ctttaaagcg tggctcgttt
      541 cgcatcaaag gcgaatcatt ggatatcatc ccgccttatg aaaaagacca ggctatccgt
      601 ctagagttct ttgataatga aatcgaaaaa attaagattg ttgatacagt aaccggacga
      661 gccttagttg aaaaaacgat gaccaacatt tttccggcca ccctttttgc catcggtgaa
      721 gctaaattaa aagaagctat tacccggatt aaagcggaat tagcggaacg gattttgttt
      781 tttgaaagcc aaaaaaagcc cttggaagcc gaacgcatca aacaaagaac ggaatatgat
      841 ttagaaatgc tggaagaagt tggcagttgc agtggtgtcg aaaactattc ccgccatctt
      901 agtttaaggg ctgaagggga aactccctat accttgttgg atttctttaa aaaggacttt
      961 ttgttagttg ttgatgaatc tcatgttact ttaccacaag ttcgggggat gtaccatggt
     1021 gatcgttctc gcaaacaaag tttggttgat tacggttttc gtcttccttc cgctttggat
     1081 aatcgaccac tgaccttttc ggaattctat agcaaactcg atcgcatcat ttatgtcagt
     1141 gcgactccaa gtgattttga aaagaagcgt agccatagca tcgtcgaaca gattattaga
     1201 ccgacgggac tccttgatcc cttggtggaa gtccgaccga ttgagggcca gattgatgac
     1261 ctcattaaag aaatcaatca gcggatttca gcaaacgagc gcattttgat tttaacttta
     1321 accattaaaa tgagcgaaga tttaagtaaa tatttaaaag aactggatta taaagttgcc
     1381 tatttacatt cggaaattaa atcattggaa agaatagaaa tcattcgcgc tttacggcag
     1441 ggaaaatatg atattttagt gggcatcaat ttattaagag aaggattgga tattccggaa
     1501 gtatccttga ttgccatttt ggatgccgat aaagaaggtt tcttgcggag cgaaacttcg
     1561 ctaattcaaa ccatcggtcg ggcggctcgg aatgccaacg ggaaagttat tttatatgct
     1621 agtcaaatga cgggatccat ggaaagagca attgaagaaa cttatcgtcg tcgttctatc
     1681 caagatgaat ttaaccaaaa acatcacatc gtgccgaaaa cgatttataa acatatcagt
     1741 gacaatattg tgattactag agaaattaaa gatgagaaga gttttgtgga tatgagttca
     1801 ctcaaagata tgagcacact ggaaaaacgc catttgatta tgcgtttaga aacacaaatg
     1861 aaggaagccg ccaaaaaact tgatttcgaa caagcgatgg ctttacgcga tattattttt
     1921 gaattaaagg cggatttacg gtaaagaaaa tgaagaaaaa agtaattgtt ggtttatcag
     1981 gcggtgttga cagtgccgtt gcctgcatta atctattaga acagggttat gatgtgaccg
     2041 ggatttttat gcgtaattgg gattcttcac tgaacaatga cattttaggt aatcctcatt
     2101 tattggatga tgtttgtccg caggaaaagg attatttgga tgccatggaa gtagcgaata
     2161 aactggggat tcctttttta cggattgatt ttgttcagga gtattgggaa agtgtttttc
     2221 aacatttttt aaacgaatac cgtctcaacc gcacccctaa tccggatatc atttgcaata
     2281 aagaaattaa gtttaaagct tttttaaata aggccgttct tttaggagct gattacatcg
     2341 ccatggggca ttatgcccgc actgtttttg aaccggaagt aaaactgtta cgcggccttg
     2401 atgctatgaa agaccaaagt tattttttat gtcagttaag taaggagcaa ctaaaaaaaa
     2461 ccctctttcc catcg
//
LOCUS       DAOSGN010000094         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_41292_length_2474_cov_2.949566, whole genome shotgun sequence.
ACCESSION   DAOSGN010000094 DAOSGN010000000
VERSION     DAOSGN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41292_length_2474_cov_2.949566"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /gene="nagZ"
                     /locus_tag="PK087_01900"
     CDS             complement(<1..1036)
                     /gene="nagZ"
                     /locus_tag="PK087_01900"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOH18056.1"
                     /translation="MISLAQMTLKEKIGQLVFVGFPKTTIDDNLKRLISEYKLGNIVL
                     FARNIANIEQLHGLNQALYQRIMNETGIMPLIAIDQEGGMVLRITEEATFWPGNMTLA
                     ASGGAHQAYEQGKMMGEELRALGINMNLAPSLDVNNNPLNPVIGVRSYGDDPKKVSEW
                     GVSAILGMQETGIIATAKHFPGHGDTSSDSHYQLPLIPHDLKRLNAVELYPFKEAIKN
                     KVKAIMSAHVFFTAYEQEQLPATLSKRVMTDLLRTELQYEGLVVSDCMEMKAIDDFYT
                     ASQGALRGLLAGLDMVMVSQTYEKQKQTLEVIEQAVLDGTFPMTLLDEKVARVLKAKK
                     ALLPVMEKHFL"
     gene            complement(1052..2191)
                     /locus_tag="PK087_01905"
     CDS             complement(1052..2191)
                     /locus_tag="PK087_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOH18057.1"
                     /translation="MKLGIDLIDQYLDVFKGKRVGLMTNTSGVNSQLQSTINIMKAKT
                     NLVALFAPEHGVRGNFQAGEKFLTYTDEETGVLVHSMYGENRRPTKEMLDQIDILCID
                     IQEVGSRFYTYIYSMAYAMEACKEYQKTMVVFDLPNPINGVDVEGNILDFNYRSFIGY
                     YPIVQRYGMTIGELALMFNEEDNIRCDLKIIPMKGWRRKMYFEETGLSWVFPSPNLPT
                     IDSTIVYNGTCIFEGTNISEGRGTTVPFQLVGAPFIKPHELAKALNDQQLKGVIFRPQ
                     YFTPSFSKYAGTLCAGVFVHVLNRRSFEPVKTGWTMLEILRQLYPQEVKILPPFRENR
                     PCMLELNTGCDYIKAQKYSLAELALILKEDTQKFKQTRKKYLIYK"
     gene            complement(2202..>2474)
                     /locus_tag="PK087_01910"
     CDS             complement(2202..>2474)
                     /locus_tag="PK087_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18058.1"
                     /translation="QLPMIVANFRMKTWSLLKFAFFMAFRYFFKTLTYGLLLLFPLLL
                     VGLLKQIFLPFYLLIGISGPLYLIYMISRKQYWYLTHNLDELTTKE"
BASE COUNT          742 a          530 c          462 g          740 t
ORIGIN      
        1 ttaagaaatg tttttccatt accggcaata aagctttctt cgcttttaat actcgggcaa
       61 ctttttcatc gaggagtgtc attgggaaag ttccgtctaa caccgcttgt tcaatcactt
      121 ccaaggtctg tttttgtttt tcataagttt gggaaaccat caccatatcc aaacctgcca
      181 acaaccctcg taaagcccct tggctggcgg tataaaaatc gtcaatggct ttcatttcca
      241 tgcagtcact aacgaccaaa ccttcatatt gcaattccgt tcgcaataaa tccgtcataa
      301 cccgtttgga taaagtagct ggcaattgtt cttgttcata ggccgtaaaa aaaacatggg
      361 cggacataat ggctttcacc ttgtttttga tggcttcttt aaaaggatat agttctaccg
      421 cattcagtct ttttaagtca tgaggaataa ggggcagttg gtaatgacta tcggatgaag
      481 tatcaccatg gcccggaaaa tgtttggctg tagcgataat gccggtttct tgcatcccca
      541 aaatggccga aactccccac tcactaacct ttttaggatc atcgccatag ctccgaacgc
      601 cgataaccgg gtttaagggg ttattgttga catcaagcga gggcgctaaa ttcatattga
      661 tgcccaaggc tcgtaattct tctcccatca ttttcccttg ttcataagct tggtgagctc
      721 ctccactagc cgccaatgtc atattgcccg gccagaatgt cgcttcttcg gtaatgcgca
      781 aaaccatgcc cccttcttga tcgatggcga ttaaaggcat aatcccggtt tcattcatga
      841 ttctttggta aagagcttgg tttaaaccgt gaagttgttc aatattggca atattacgag
      901 caaataaaac aatatttccg agtttatact cggaaataag ccgttttaaa ttgtcatcaa
      961 tcgtagtttt cggaaaaccg acaaacacta attggccgat tttttctttc aaggtcattt
     1021 gtgctaacga aatcatccag ttctcctttg tttatttgta aattagatat tttttacgcg
     1081 tttgtttaaa cttctgggtg tcttctttta aaataagcgc cagttccgct aaagaatatt
     1141 tttgggcttt gatataatca cagccggtat tgagttctaa catgcagggt ctgttttcac
     1201 ggaaaggcgg aagaatttta acttcctgag gatacaattg gcgtaaaatt tccagcatcg
     1261 tccacccggt tttcaccggt tcaaatgaac ggcgattcaa aacatgaacg aaaacaccag
     1321 cacacaaagt acccgcatat ttggaaaaag acggcgtaaa atactgtggt cggaaaataa
     1381 cgccttttaa ttgctgatca tttaaggctt ttgccagttc atgcggttta ataaacggcg
     1441 ccccaacaag ttgaaaagga accgttgtac cgcgaccctc cgagatattc gtgccttcaa
     1501 aaatacaagt gccgttatac actattgtgg aatcaatggt cggtaaattg ggggagggaa
     1561 aaacccacga caagcccgtc tcttcaaagt acatcttgcg gcgccatccc ttcatcggaa
     1621 tgatttttaa atcacaacga atattatctt cctcgttaaa catcaaggcc aactccccaa
     1681 ttgtcatgcc ataacgttgg acaatcgggt aataaccgat aaaggaacga taattaaaat
     1741 caaggatatt gccttccaca tccacaccat taatcggatt cggtagatca aaaacaacca
     1801 tcgttttttg atattccttg caagcttcca tagcataggc catggaatag atataggtat
     1861 aaaaacgcga tccgacttct tgaatatcga tgcataaaat atcgatttgg tccaacatct
     1921 ctttggttgg gcgtcgattt tcaccataca tactgtgaac taaaacgccg gtttcttcat
     1981 cagtataagt gagaaatttt tctccggctt ggaaattgcc acgaacacca tgttccggag
     2041 caaagagcgc caccaaattt gttttcgctt tcataatgtt aatcgtcgat tgtaattggc
     2101 tgttgacccc ggaagtgttg gtcattaagc ccactcgttt gcctttaaaa acatctaaat
     2161 actgatcaat taagtcaatt cctagtttca taaacccttc ctcattcttt tgttgttaac
     2221 tcatctaaat tgtgagtcaa ataccaatat tgtttacggg aaatcatata gattaaataa
     2281 agtggtccac tgatgccgat taataaataa aagggtaaaa aaatttgttt taacaagccc
     2341 actaataaca aggggaaaag cagtaataag ccataagtta atgttttaaa aaaataacga
     2401 aacgccataa aaaaagcaaa tttaagcaaa ctccatgttt tcatccgaaa attggcaaca
     2461 atcatcggga gttg
//
LOCUS       DAOSGN010000095         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_41353_length_2471_cov_3.846026, whole genome shotgun sequence.
ACCESSION   DAOSGN010000095 DAOSGN010000000
VERSION     DAOSGN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41353_length_2471_cov_3.846026"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2143
                     /locus_tag="PK087_01915"
     CDS             <1..2143
                     /locus_tag="PK087_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18059.1"
                     /translation="SVPWFRDGVLIVVDATGKFVLARIMTTSAAAEVRADGTVIYGNA
                     AVVAPTEADKAKAGDPNLTWDLVIPNANNTVPHGPLADIQNVIPEGGAFFILPGTSNT
                     AVRNYGIKLLWNNSYPGSGIIVDASLPEQPAGAPNPATNGFDSTNYNAAYLAGLTFKV
                     QFVSTVTAKPTKIVRPVATITEHILAWEQVANAGSYDVYVDGVLKASNVGELVPDTTT
                     YNYDLSLLKLDGQVHTIQVRAITANAQTASTSVLSDPVTVQFIALTAPIKLALDDKVL
                     SWAVVDKAVSYEIYVNDVLAGTSATNSFDLSEFFGSSAKSVQVKALPAAADKMYVASP
                     LSTKFSFGVPTSTYLKLALESQHITYTTVDEWMTKRNITVADGGFAGSTEIFYIEDAV
                     KFATFATQTAVVTANGTFLLFGADGKLKAVRTIVGTFEWLATNDANVNNGWRTLSDVT
                     GGQVKVQEIRSLVAEGDFIILSNNTAKKQEGAALSPRDFLAYHFMGAWTIGEQETWRK
                     TIDQFINPSTVTYKLSIYPLTYYLALTLESQHITYTTVDEWMTKRNITVADGGFAGST
                     EIFYIEDAVKFATFATQTAVVTANGTFLLFGADGKLKAVRTIVGTFEWLATNDANVNN
                     GWRTLSDVTGGQVKVQEIRSLVAEGDFIILSNNTAKKQEGAALSPRDFLAYHFMGAWT
                     IGEQETWRKTIDQFINPSNVTYTFKKVDVTF"
     gene            2212..>2471
                     /locus_tag="PK087_01920"
     CDS             2212..>2471
                     /locus_tag="PK087_01920"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HOH18060.1"
                     /translation="MKFEKLYNSKFYMFFECLYRLILVNLLFILCSLLGLVVFSVVPA
                     AIALVTVIKSYSSETAFPIGKTFWHIFKKHFKSNLVLSGLFFV"
BASE COUNT          706 a          492 c          526 g          747 t
ORIGIN      
        1 aagtgtacct tggttccgtg acggtgtctt aatcgttgtt gatgcaacgg gtaaatttgt
       61 attagcgcgg attatgacga cttccgcggc ggccgaagtt cgtgctgacg gtactgtcat
      121 ttatggtaat gctgcggtag ttgctccaac ggaagcagat aaagcaaagg ccggagatcc
      181 gaacctaact tgggatttag ttattccaaa tgctaacaac acggttcctc atggtccatt
      241 agccgacatt caaaatgtta ttcctgaagg tggagctttc ttcattcttc cgggaacaag
      301 caatacggcg gttcgtaatt atggaattaa attgctttgg aataatagtt atccgggcag
      361 tggtattatt gttgacgcaa gcttgccgga acaaccagca ggtgcaccta acccagccac
      421 caacggcttt gattccacaa attacaatgc tgcttacctt gccggtttaa ctttcaaagt
      481 tcaatttgtt tcaacagtta ctgctaaacc aactaaaatt gttcgtccgg tagcaacgat
      541 taccgaacac atcttagcat gggaacaagt tgctaatgcc ggctcatatg atgtttatgt
      601 tgacggtgtt ttaaaagcaa gcaatgttgg tgaattagta ccggatacga cgacttataa
      661 ttatgattta agtttattaa aactagatgg tcaagttcat acgattcaag taagagcaat
      721 tacggccaat gcgcaaacgg cttccacttc ggtcctttcg gatccggtga ccgttcagtt
      781 catcgctttg accgcaccta ttaagttagc tcttgacgat aaggttcttt cttgggcagt
      841 tgttgacaaa gccgttagct atgaaattta tgtcaatgat gttttagcgg gaacttccgc
      901 aaccaacagt tttgacctaa gcgaattctt cggttcttcc gcaaaatctg ttcaagtaaa
      961 agccctaccg gcggctgctg ataagatgta tgttgcttcg ccattatcta ccaaattctc
     1021 atttggtgta ccgacgagca catatttaaa attagcgctt gaaagtcaac atataactta
     1081 tacaacggtt gatgaatgga tgaccaaacg gaatattacc gttgctgatg gtgggtttgc
     1141 cggttcaacg gaaatcttct atattgaaga tgctgttaaa tttgcgacct tcgccactca
     1201 aaccgctgtt gttaccgcca atggaacctt cttactattc ggtgctgatg gcaaattaaa
     1261 agctgttcgc accatcgttg gtacttttga atggttagca acgaatgatg ccaatgttaa
     1321 taatggttgg agaacattaa gcgacgttac aggtggacaa gttaaagttc aagaaattag
     1381 gtccttagta gcggaaggcg acttcattat tttatcgaat aacacggcca aaaaacaaga
     1441 aggagcagct ttaagtccgc gtgatttctt agcatatcac ttcatgggtg cttggacgat
     1501 tggcgaacag gaaacttggc gtaagacgat tgatcaattc atcaatccgt cgacggtcac
     1561 ttacaaacta tcaatttatc cgctgactta ctatttagca ctaacccttg aaagtcaaca
     1621 tataacttat acaacggttg atgaatggat gaccaaacgg aatattaccg ttgctgatgg
     1681 tgggtttgcc ggttcaacgg aaatcttcta tattgaagat gctgttaaat ttgcgacctt
     1741 cgccactcaa accgctgttg ttaccgccaa tggaaccttc ttactattcg gtgctgatgg
     1801 caaattaaaa gctgttcgca ccatcgttgg tacttttgaa tggttagcaa cgaatgatgc
     1861 caatgttaat aatggttgga gaacattaag cgacgttaca ggtggacaag ttaaagttca
     1921 agaaattagg tccttagtag cggaaggcga cttcattatt ttatcgaata acacggccaa
     1981 aaaacaagaa ggagcagctt taagtccgcg tgatttctta gcatatcact tcatgggtgc
     2041 ttggacgatt ggcgaacagg aaacttggcg taagacgatt gatcaattca tcaatccgtc
     2101 caatgttacc tataccttta agaaagtcga tgtcactttc taatattgat caatgattaa
     2161 gtgaataagt cgaggagcaa tcctcgactt atcgctaatc aggagaaaaa gatgaaattt
     2221 gaaaaattat acaacagtaa gttctatatg ttttttgaat gcttatatcg gctaatcctc
     2281 gtcaatttgt tattcattct ttgctcattg ctgggattag tcgttttttc ggttgtgccg
     2341 gcagcgatcg cccttgtaac cgtaattaag tcatattctt ccgaaaccgc ttttcccatt
     2401 ggaaaaacct tctggcatat ttttaaaaaa cactttaaaa gcaatctcgt tttatcaggt
     2461 ttattcttcg t
//
LOCUS       DAOSGN010000096         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_41842_length_2451_cov_2.597245, whole genome shotgun sequence.
ACCESSION   DAOSGN010000096 DAOSGN010000000
VERSION     DAOSGN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41842_length_2451_cov_2.597245"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PK087_01925"
     CDS             <1..276
                     /locus_tag="PK087_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126794041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOH18061.1"
                     /translation="SQIQNREKAMNLLKAKLYQLEQEEQDNKIRAIAGQVSENTFGSQ
                     IRSYVFHPYSMIKDHRTDFEVGNVAPVMDGDIDGFINAYLKSEFNVR"
     gene            279..1169
                     /locus_tag="PK087_01930"
     CDS             279..1169
                     /locus_tag="PK087_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOH18062.1"
                     /translation="MHPLNKAKKWRKRLIEISFITLGVAIAAFAFSFFLNVNTIVIGG
                     GSGIGVILADLFGFDPSLVILILNVFLLILGLFLLGKEFFIKTIYGSLAYPVFVKIFD
                     CLYQWLQVDFSAQALDMVLIIFFSSIIMGFGLGLVMKFGGTTGGTEVGQKILMKYFHI
                     PLSTSLYFLDGIVILGGLLFKVIDIELALYAVVFTYLCGVVIDSVVFTGINKRAVYII
                     SNKNEEIKKDLLEKFERGVTSIRVVGEYSKQDREMLICVLSISEYYRLREIIEDYDPQ
                     AFYYSVRASEVRGEGFSYDK"
     gene            1159..1941
                     /locus_tag="PK087_01935"
     CDS             1159..1941
                     /locus_tag="PK087_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOH18063.1"
                     /translation="MTNEIQILSLTKKKKKWIVETKEKKYSFHEDTVIKYYLFKDKSF
                     TEEEWKQVLTYEAVADLMEKTLKFLSYQRRSTKEVQKFLSSKTDQPLFIEQVLMKIHQ
                     LGYLNDEKYAMEVLDYEKRHQKGPKVVFQKLQQKGLDETLINRVLLNYDDVTEQDILD
                     EVLEKLVQKNQDQPVLKQKNIIMQKLLRDGFSQSLILEGLKKVSFLDKSANKLVLEIE
                     KYQHRYQNLPNQERISKIIKHLMTKGYSYAQIREQLKQNEGE"
     gene            2038..>2451
                     /locus_tag="PK087_01940"
     CDS             2038..>2451
                     /locus_tag="PK087_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18064.1"
                     /translation="MKVLRKLILIVLVVINLIFIGGLANLQAVDLVPIPKSGGGNVTY
                     RSTSIEVKMPSAYIPQETDFRAVWISPLVGDLAPFGSESQFKKQMNVIFDTLEYFNMN
                     AMVFHMRLNNDALYNSELNPLSYVFRNVNFAEFDPI"
BASE COUNT          773 a          365 c          484 g          829 t
ORIGIN      
        1 agtcaaattc agaatcggga aaaagcgatg aatttattga aagcgaagtt gtaccaactt
       61 gaacaagaag agcaggacaa taaaattcgg gcgattgccg gtcaagtttc tgaaaacacc
      121 tttggtagcc aaattcgttc ctatgttttc catccttatt ctatgattaa agaccaccgt
      181 actgattttg aggttggtaa tgtggcaccc gtcatggatg gtgatattga tggctttatc
      241 aatgcttatc ttaaatcgga atttaatgtg aggtaaatat gcatccatta aataaagcga
      301 agaaatggcg aaaaagactt attgaaatct cgttcattac tttaggcgtg gcgattgccg
      361 cttttgcttt tagcttcttt ttgaatgtca atacaattgt tatcggtggt ggttccggaa
      421 tcggggttat tttggcggat ttatttggct ttgatccctc attagtcatt ttaattttaa
      481 atgtgttttt attgatatta ggtttatttt tactggggaa agaatttttt attaaaacta
      541 tctatggttc attggcttat ccggtgtttg tgaagatatt tgattgctta tatcaatggc
      601 ttcaagtaga tttttctgct caagcactcg atatggtttt aattatcttt ttttcatcga
      661 ttattatggg atttggtttg ggtttggtga tgaaatttgg cggtactact ggggggacgg
      721 aagttggtca aaaaattctc atgaaatatt ttcacatccc cctttccaca tcgctttatt
      781 ttcttgacgg tattgttatt ttagggggct tattatttaa agttattgat attgaattag
      841 ccctttatgc ggttgtcttt acttatttgt gtggagtggt gattgattcc gtggtcttta
      901 ccgggattaa taaaagagca gtttacatca tctccaataa aaatgaagaa ataaaaaaag
      961 atttgttaga aaaatttgaa cgtggtgtca ccagcatccg cgttgtgggt gaatattcca
     1021 aacaagatcg agaaatgctg atttgtgttc tttccatttc cgagtattat cgattgcgag
     1081 aaatcattga agattacgat ccccaagctt tttattactc tgttcgtgcc agtgaagtcc
     1141 gtggggaagg cttttcctat gacaaatgag attcaaatct taagtttaac caagaagaag
     1201 aaaaaatgga ttgtggaaac gaaggaaaaa aaatattcct ttcatgaaga tacggttatt
     1261 aaatattatc tttttaaaga taaaagcttt acggaagagg aatggaaaca agtattaact
     1321 tatgaagcgg tggctgattt aatggaaaaa accttaaagt ttctaagtta tcaaaggcgc
     1381 tccactaaag aagtacaaaa gtttttatct tcgaaaacgg atcaaccctt gtttatagaa
     1441 caggtgttga tgaaaattca ccaattaggt tatttgaatg atgaaaaata tgccatggaa
     1501 gtccttgatt atgaaaaacg tcatcaaaaa ggtcccaagg tggtttttca aaagctacaa
     1561 caaaagggtc ttgatgaaac tttaatcaat agagttttac ttaactacga tgatgtaact
     1621 gaacaggaca tccttgatga agtcctggaa aagttagttc aaaagaacca agatcaaccc
     1681 gttttgaaac aaaaaaacat cattatgcaa aaactgttgc gggatggttt ttcgcaatct
     1741 ttaattttgg aaggactaaa aaaagtttct tttcttgaca aaagtgcaaa taaattggtt
     1801 ttagagattg aaaaatatca acatcgttac caaaatctac ctaatcaaga acggatttca
     1861 aaaataatca agcatttaat gaccaagggt tattcgtatg cccaaattcg cgagcaatta
     1921 aaacaaaatg aaggggaata aattcctagg ggaattattt ccgggggaat tatttcccct
     1981 ttttatttac ttaaggtcga aaaaatgcta taatcgtttt acataaggag tagcaaaatg
     2041 aaagtgttaa gaaaattaat tttgattgtt ttagtagtca tcaacctcat ctttattggt
     2101 ggtttggcta atttacaagc tgtggattta gttcccatcc cgaaaagtgg cggtgggaat
     2161 gtcacttacc gttcaacctc cattgaagta aaaatgcctt cagcatatat tcctcaagaa
     2221 acggattttc gagcggtttg gattagtcct ctcgttggtg atttagcacc ttttggtagt
     2281 gagtcccaat ttaaaaaaca aatgaatgtt atttttgata cattagaata ttttaatatg
     2341 aatgccatgg tttttcatat gcgcttaaat aatgacgcgc tttacaattc agaactcaac
     2401 cccttatcct atgtttttcg gaatgttaat tttgcggagt ttgacccgat t
//
LOCUS       DAOSGN010000097         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_41924_length_2448_cov_2.266611, whole genome shotgun sequence.
ACCESSION   DAOSGN010000097 DAOSGN010000000
VERSION     DAOSGN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41924_length_2448_cov_2.266611"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PK087_01945"
     CDS             <1..301
                     /locus_tag="PK087_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18065.1"
                     /translation="DSASGSLETVLGSDFVDYNAFDTTNPEQQQAVEELNQTLDDIQN
                     VLNDPEQELTMEDTTALIEAINNLDEANQQIFLDQLENSSGSADLLALYDDLFGN"
     gene            314..889
                     /locus_tag="PK087_01950"
     CDS             314..889
                     /locus_tag="PK087_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOH18066.1"
                     /translation="MEKKILVETSARHVHVSDADLKTLFGPNAQLTPKKMLSQPGQFA
                     AEEKVTIVGPKKSIPNVSILGPSRKKTQVEVSATDARTLGFEIVIRESGDIEKTPGCT
                     LVGPYGQVEIPEGVIVAKRHIHLNLEEAKAFGLQDKQVVMVKVNTPDRSLIFGDVVVR
                     VRSDFAAAMHIDTDESNAGCVGMSTWGEIIE"
     gene            987..1205
                     /locus_tag="PK087_01955"
     CDS             987..1205
                     /locus_tag="PK087_01955"
                     /inference="COORDINATES: protein motif:HMM:NF021988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOH18067.1"
                     /translation="MNLKKLVLRAEAKFMKTSMLLFFAPGRVNLIGEHLDYNGGLVLP
                     CAIDLGIYGVVAPRDDALVRCFSEMCLF"
     gene            1193..2137
                     /gene="galK"
                     /locus_tag="PK087_01960"
     CDS             1193..2137
                     /gene="galK"
                     /locus_tag="PK087_01960"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOH18068.1"
                     /translation="MPFLGVIEFSLSQLMYEKKHGWANYVKGMLKETKATCGFDLYVR
                     GNLPNGAGLSSSAALEILVGTIVNRLENRQMSATAIALCGQKAENNFIGVQCGIMDQF
                     VIAHAQEGHAMLLDTSNLSFSLVEANFLDYCLVIGNTNKRRALSDSKYNERRSECEQG
                     LRDLNTVLDIPYLCALKAADLDKYAYLIDNPVVFKRVRHVVTENERVKKAFEALKLRN
                     LQEFGKLLNASHLSLKHDYQVTGLELDTLVALHQAYGAIGARMTGAGFGGCMIALMPK
                     AHLNEVLREISQEYQQITHLVPDFYLVTAGKGANQYGY"
     gene            2127..>2448
                     /locus_tag="PK087_01965"
     CDS             2127..>2448
                     /locus_tag="PK087_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOH18069.1"
                     /translation="MDINYHINELLNYGLSKALMTKCDYTYNANVLIALFNRKEFIAI
                     DTPPRDLSLILDDLAHFAFHNHLIPSNDVVTMDNFKAAIMDAIMEKPQTVIDRFQELY
                     QQKPS"
BASE COUNT          724 a          429 c          534 g          761 t
ORIGIN      
        1 tgattcagca tcggggagtt tggaaacagt tttaggctcg gactttgttg attataatgc
       61 ttttgatacg accaaccccg aacaacaaca ggctgttgaa gaattaaacc aaaccctaga
      121 tgatattcaa aacgtcttaa atgacccaga acaagaatta accatggaag atacgaccgc
      181 tttaattgaa gcaatcaata atttagatga agccaatcaa caaattttcc ttgatcaatt
      241 ggaaaattcg agtggttcgg ccgatttatt agctttatat gatgatttat ttggaaatta
      301 agaggagaag aaaatggaaa agaagatttt agtagagaca tcggctcgcc atgtccatgt
      361 gtcggatgcc gatttaaaga ctttatttgg ccccaatgct caattgaccc ccaaaaaaat
      421 gttgtcccaa cctgggcagt ttgctgctga agaaaaggta acgattgttg gtcccaagaa
      481 aagcatccct aatgtttcaa tattaggacc aagccgtaaa aaaacccaag tggaagtatc
      541 ggctaccgat gccagaacat tgggatttga aattgtcatt cgtgaatcgg gtgatattga
      601 gaaaactccc ggctgtactc ttgttggtcc ctatgggcaa gtggaaatcc ctgaaggtgt
      661 tattgttgct aagcggcata tccacttaaa ccttgaagaa gctaaggctt ttggtttaca
      721 ggacaaacaa gttgtcatgg tgaaagttaa cactcctgac cgttcgctta tttttggtga
      781 tgtcgttgtt cgtgttcgca gtgattttgc ggcggcgatg cacattgata ccgatgaatc
      841 caatgcgggc tgtgtcggaa tgtctacttg gggcgaaatt attgaataag cttttggttc
      901 taagcacatc gatgcttaga acctttttcg tttatgtcct tgaatcaaac tctatatttg
      961 agtataatta aagcaaggta aaaaatatga atttaaaaaa attagtttta cgcgccgaag
     1021 ctaaatttat gaaaacttcc atgttgctct tttttgctcc ggggcgggtt aatttaattg
     1081 gtgaacattt ggattataac ggtggattag tgttaccttg tgccatcgac ttaggaattt
     1141 atggagtcgt ggccccaagg gatgatgccc ttgtgcgctg tttttcggaa atgtgccttt
     1201 tttaggagtt attgagtttt cgctttcaca actgatgtat gaaaaaaaac acggttgggc
     1261 caattatgtg aaaggaatgc taaaggaaac caaggccact tgcggttttg atttatatgt
     1321 tcgaggtaac cttcccaatg gggcaggact ttcatctagt gctgctttgg aaatattggt
     1381 gggaaccatt gttaaccggc ttgagaaccg ccaaatgtcg gcgaccgcca tcgctttatg
     1441 cggacaaaaa gccgagaata actttattgg tgtgcagtgt ggcattatgg atcaatttgt
     1501 gattgctcat gctcaagaag gccatgcgat gcttttggat accagcaatt tatctttttc
     1561 tttagttgag gcgaactttt tggattattg cttagtcatc ggtaatacca acaaaagacg
     1621 ggccttaagt gattctaaat ataatgaaag aagaagtgaa tgtgaacaag gtcttaggga
     1681 tttaaatacg gttttggata ttccttattt atgtgcttta aaggcagctg atttggataa
     1741 atatgcttat ttaattgata accccgttgt ttttaaacgg gttaggcatg tggtgacgga
     1801 aaacgaacgc gtcaaaaaag cttttgaagc tttgaaatta cgcaatttac aagaatttgg
     1861 aaaactttta aacgcttccc atttatcatt aaaacatgat tatcaagtaa cgggtttaga
     1921 actggatacc ttggtggcct tgcatcaagc ctatggagca attggcgctc ggatgaccgg
     1981 tgccggtttt ggtggttgta tgatagcttt aatgcctaaa gctcatttaa atgaagtctt
     2041 aagagaaatc agtcaagagt atcaacaaat aacccattta gtccctgatt tctatcttgt
     2101 gactgccgga aaaggagcta atcaatatgg atattaatta tcatattaat gaattattaa
     2161 attacggttt gagtaaagcc ttgatgacta aatgtgatta tacctataat gccaatgttt
     2221 taattgcttt gttcaatcgt aaggagttta tcgctatcga tactccaccc cgtgacttaa
     2281 gccttattct tgatgatttg gcccattttg cctttcacaa tcacctcatc cccagtaatg
     2341 acgtcgttac gatggataat tttaaagcgg ctatcatgga tgccattatg gaaaaaccgc
     2401 aaacggttat tgaccgtttt caggaactgt atcaacaaaa accgtcct
//
LOCUS       DAOSGN010000098         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_42707_length_2417_cov_2.648603, whole genome shotgun sequence.
ACCESSION   DAOSGN010000098 DAOSGN010000000
VERSION     DAOSGN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42707_length_2417_cov_2.648603"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PK087_01970"
     CDS             complement(<1..401)
                     /locus_tag="PK087_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18070.1"
                     /translation="MSSILNINRFIIPNDVIVTIAKLNRWIGQNQKFLDIVKTDVERI
                     VEQTIERDSYFLSKILSLDISDARTRLIITKNSAPRTKDETTLFNVKEMLTLFQQKHA
                     TLNYQSNDLLNLVNSLFPHYPTVKFDYSSTD"
     gene            complement(508..>2417)
                     /locus_tag="PK087_01975"
     CDS             complement(508..>2417)
                     /locus_tag="PK087_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOH18071.1"
                     /translation="YATEIPPHNIDEVIDAVIYLIDHPDASISDLMKIINGPDFPTGG
                     IVQGIDGIRKAYETGRGKISLNAKIEIVEGDNLNQLVITEIPYEVNKAMLVKKMSEVY
                     VQKNVDGIMDIRDESDRQGLRIVVDLRKDANPEFIRNFFMKSTELSVNYNFNMVAIAN
                     KRPMLMNLRDLLSAYIDHQREVITNRSNFELNQAKRRLHIVSGLIAMTSIMDSVIQTI
                     RNSANKKDAKDNLVSNYNFSEEQAEAIVMLQLYRLTNTDIVMLREEEENLKALVIKLS
                     EILSNENILLKTIKDELKQTKQVLTTPRKSIIEHEIEELKVEVKELIAKEDAVVIITN
                     DGYIKRMTPKVFANNEETKLKDEDVVIATYNVTTTDTLLLFTNLGNYVFLPITKIPEV
                     KHKDLGYNVSTLASIEANEKIIFTVPIDDFEKERYLLFTTKNGLIKRTLVSEMNANRY
                     SRALKATKIRPEDELVSVDISSEERTEVVVVTKEGFINRYDASEISIMAPPSFGVKAI
                     ELKNRPYDEVVGGHFVCAKDIIVILTLKAAIKRLRPEEIVKGRKNNVGKQYLTITKTS
                     PNFALSSQIIHRKNANLNLDCYLFGLKGYVPVDFNTVKNATAQSGKKITNKDIGKPDK
                     LIIVRNNNDFE"
BASE COUNT          729 a          433 c          388 g          867 t
ORIGIN      
        1 ttgtcagttg atgaatagtc aaattttaca gttggataat ggggaaaaag gctattgact
       61 aaattcaata aatcattact ttgataattt aaagtagcat gtttttgttg aaacaaagtt
      121 agcatttctt tcacattaaa aagcgttgtt tcatctttgg ttcttggtgc cgaatttttg
      181 gtaataatta aacgagtcct ggcatcgcta atatctaagc taaggatttt tgataagaaa
      241 tagctatccc tttcaatcgt ttgttcaaca attcgctcga catcagtctt cacaatatcg
      301 agaaattttt ggttttgacc aatccaacga tttagtttag caatagtaac aataacatca
      361 ttaggaataa taaatcggtt aatatttaga attgagctca tttctctctt cccttctttt
      421 aaaataaccc tccttaaatc gcacgatgaa ttattattca acttttaaaa gataatcgct
      481 ttacaggagg gtttgataat gcttttttta ctcaaaatcg ttgttattgc ggacaattat
      541 cagtttatcc ggctttccga tgtctttatt cgttattttt ttgccggatt gcgcagtggc
      601 atttttaact gtattaaaat caaccggcac ataacccttt aagccgaata agtaacaatc
      661 taaattgaga ttggcatttt tacgatgaat gatttgtgaa gacaaggcaa aattaggcga
      721 agttttcgtg attgttaaat actgtttccc aacattgttt ttacgccctt taacaatttc
      781 ttcgggacgg agacgtttaa ttgcagcctt taaagttaaa ataacaataa tatctttcgc
      841 acaaacaaaa tgaccgccaa ccacttcgtc ataaggccga ttcttcaact cgatggcttt
      901 gacaccgaac gaaggtggtg ccataatact gatttcactg gcatcataac gattaataaa
      961 tccttctttg gtaacgacaa ctacttccgt tcgttcttcc gatgaaatgt caacactgac
     1021 taattcatct tcagggcgga ttttagtcgc ttttaaagct ctagagtagc ggttggcatt
     1081 catttcactt actaaggttc gtttaattaa accgttttta gttgtaaaca ataaataacg
     1141 ttctttttca aaatcatcta tcggtacggt aaaaataatt ttttcattag cttcaatgga
     1201 agctaaagta ctaacattat aacctaaatc cttatgtttt acttcaggaa ttttagtaat
     1261 cggtaaaaaa acataatttc ccaaatttgt aaataacaac aaagtatccg tggtcgttac
     1321 attataggtg gcaataacaa catcttcatc tttgagtttc gtttcttcat tgttggcgaa
     1381 aactttagga gtcatgcgtt ttatataacc atcattcgtg atgattacaa cagcatcctc
     1441 tttcgcaatc aattctttaa cttcgacttt taattcttca atttcatgtt caattattga
     1501 tttccttggg gttgttaaaa cttgtttggt ttgttttagt tcatctttga tagttttaag
     1561 taaaatattt tcgttggata gaatttccga taattttata actaaggctt ttaagttttc
     1621 ttcttcttca cgaagcatga caatatcggt attcgtaagc cgatataact gcaacatcac
     1681 aatcgcctcg gcttgctctt ccgaaaaatt ataattactt actaaattat ctttagcatc
     1741 ttttttatta gcagaattac gaatggtttg aataacggaa tccattatgg aagtcatcgc
     1801 aattaaaccg ctgacaatat gtaaacgacg ttttgcttga ttgagttcaa aattactgcg
     1861 gttggtaatt acttctcgtt gatgatcaat gtaagctgaa agtaaatcgc gaagattcat
     1921 caacattggt cgtttattgg caatagctac catattaaaa ttataattta ccgaaagttc
     1981 cgtggatttc atgaaaaagt tacgaataaa ttccggattg gcatctttcc gaaggtcgac
     2041 aacaattctt aatccttggc ggtcggattc atcacgaata tccatgatgc catcaacatt
     2101 tttttgaaca taaacctcgc tcattttttt cacaagcatg gctttgttaa cttcgtaagg
     2161 tatttctgtg atgactagtt gattgaggtt gtcaccttcc acaatctcga ttttcgcatt
     2221 aagactaatt tttccccgtc ctgtttcata agcttttcga attccatcaa ttccttgtac
     2281 aattcctccc gtcgggaaat ccggaccatt aatgattttc attaaatcgc taatactagc
     2341 atcaggatga tcgattaaat aaatgacagc atcaattact tcgtctatgt tatggggagg
     2401 aatttcggtt gcataac
//
LOCUS       DAOSGN010000099         2388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_43531_length_2388_cov_2.734676, whole genome shotgun sequence.
ACCESSION   DAOSGN010000099 DAOSGN010000000
VERSION     DAOSGN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43531_length_2388_cov_2.734676"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PK087_01980"
     CDS             complement(<1..367)
                     /locus_tag="PK087_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HOH18072.1"
                     /translation="MKYHKLYTKYASLLDEKAPWPEYPRPQMVRHSYLSLNGTWDYYL
                     GNEASLPSHYDGQILVPFPIESLLSKVQKRVKPKEYLFYRRHFRLPEGFLKDRLLLHF
                     EAVDQKAIVFINQIKVFEHI"
     gene            complement(372..815)
                     /locus_tag="PK087_01985"
     CDS             complement(372..815)
                     /locus_tag="PK087_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18073.1"
                     /translation="MRKKIFIGIFLLVGLITLALFVVYLLQTSGVYGQYECTVYTEET
                     TGDPVNLLPNYSYYRLELKLNRTFVMTSLIKGQEEASTATGTFTKSRQTITLTYAKGQ
                     NPVEAVIFPSEVFVYRNGTLNRNQSGSFSGTSFQTTVVQEFVKKR"
     gene            1057..1284
                     /locus_tag="PK087_01990"
     CDS             1057..1284
                     /locus_tag="PK087_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOH18074.1"
                     /translation="MTTLKDIKIGRSAVVVGINATGPLRRRFMDMGITKGTVIEVIKY
                     APLGDPMELSLRDYILSIRKSDAELIIVQEI"
     gene            1351..>2388
                     /locus_tag="PK087_01995"
     CDS             1351..>2388
                     /locus_tag="PK087_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOH18075.1"
                     /translation="MIRIGLIGNPNSGKTTIFNALTGTQQDVGNWPGVTVAKKEGRIG
                     KDILITDLPGIYSLSPYSKEEVITRDYLLSHQVDVIINLVDATNLERNLYLTTQVLEM
                     SIPTIVALNMMDEVAKRQIKIDVKRLAQLLHCPVVPISAKKNQGLKDLINQAVLLAKE
                     KNVSQLVIYPLELAFTLDKIKSETKSSLFTAVKLFEGDQSFNEQGKKVKEIIASYLAK
                     QLDDGESIVANERYRFSEQVVSQVLIKKPQQTKSLSAKIDSVLTHKIWALPIFALIMF
                     IIYYTAITVVGNNVSGLMEQLLTALKTWVSQRLTAINSPLWLIDLFTNGIIGGVGSVL
                     SFLPQLFVLFLL"
BASE COUNT          747 a          424 c          491 g          726 t
ORIGIN      
        1 cgatatgttc aaagacttta atttgattga taaagacaat cgccttttgg tcgacggctt
       61 caaaatgaag caacaaacgg tcttttaaaa atccttctgg aaggcgaaaa tggcgtcgat
      121 aaaataaata ttccttcggt tttacgcgtt tttggacttt ggataagagt gattcaattg
      181 gaaaggggac gagaatttgg ccatcataat gactgggaag ggaggcttcg tttcctaaat
      241 aataatccca agtgccgttt agagataaat aggaatggcg gaccatttgc ggtcggggat
      301 attccggcca tggtgctttt tcatctaata aggaagcata tttcgtatat agtttgtgat
      361 acttcattta attacctctt ttttacaaat tcctgcacaa ccgtggtttg aaaagaagtc
      421 ccggaaaaag aaccgctttg gttgcggttt aaggtaccat tacgatagac aaaaacttcg
      481 cttgggaaaa tgaccgcttc aacagggttt tgtcctttgg catatgttaa agtgatggtt
      541 tgtcgggatt ttgtgaaagt cccggtggcg gtgcttgctt cctcttgacc tttgattaaa
      601 gaagtcataa caaaagtgcg gtttaatttt aactccaaac gataataaga ataatttggg
      661 agcagattca cgggatcacc cgtggtttcc tctgtgtaaa cggtgcattc atattgccca
      721 taaacacccg atgtttgcaa taaataaacc acaaacaaag ccaaagtaat aagaccgacg
      781 agcaaaaaga tgccaataaa aattttcttg cgcatgttga tactcctttt gaataattct
      841 ttaaatagta tagcataaaa agcttaaaaa ttaaacgctt tctgctaaaa ttgattgcat
      901 tcttggaaac tttgtgtata atattattag ttagcatata ctaacttgga ggatgctatg
      961 ccttggttta ataacagaaa acagcgaaac aaacatcttg ccggtggctt tcgtcacgga
     1021 caagaaaacc acgaaacaaa agcagttgtc aattcgataa caacgctaaa agatataaaa
     1081 attggaagaa gcgctgtggt tgtcggcatc aacgccaccg gacccttgcg ccgccgcttt
     1141 atggatatgg gcattaccaa agggaccgtt attgaagtaa tcaaatatgc tcccttaggc
     1201 gatccgatgg aattaagctt gcgtgattat attttatcga ttcgcaaaag tgatgccgaa
     1261 ttaattattg ttcaagaaat ctaaatggcg aagattagct tcgctttctt ttaaggtagt
     1321 agttaagcat agataactag aggaggaaag atgatccgga ttggtttaat aggaaatcct
     1381 aatagtggca aaaccactat ttttaatgct ttaaccggaa ctcagcaaga tgttgggaat
     1441 tggccgggag tgacggttgc gaaaaaagaa ggtcggattg gtaaggacat ccttattacc
     1501 gatcttcccg gtatctattc attatctcca tattcgaaag aagaagttat tacccgtgat
     1561 tatcttttat cccatcaagt tgatgtcatc atcaatttgg ttgatgcgac taatttggaa
     1621 agaaatttat acttaacaac ccaagttttg gaaatgtcca taccgacaat tgtcgccctc
     1681 aacatgatgg atgaagtcgc aaaacgccaa ataaaaattg atgttaagcg tttagcccaa
     1741 cttttgcatt gtcccgttgt tcccatcagt gctaaaaaga atcaaggttt aaaagattta
     1801 attaatcaag ccgttttgct tgcgaaagag aaaaatgttt cgcaactagt tatctatcct
     1861 ttagaattag ctttcacttt agataaaatc aaatcggaaa cgaaatcttc tttatttacg
     1921 gccgttaagc tgtttgaagg tgatcaatct tttaatgagc aagggaaaaa agtcaaggag
     1981 attattgctt cttatttagc aaaacaacta gatgacggag aaagcatcgt tgccaatgaa
     2041 cgatatcggt tttccgaaca agttgtttcg caggttttaa tcaaaaaacc gcaacaaacg
     2101 aagtccttat cggcaaaaat cgactcagtg ttgactcata aaatttgggc cttaccgatt
     2161 tttgcactca ttatgtttat catttattac accgctatca cggtggttgg aaacaatgtc
     2221 agcggcttaa tggaacaatt gttgactgct ttaaagacat gggtgagtca gcgcttaacc
     2281 gccattaaca gtccgctttg gttaattgat ctgttcacaa acggcattat tggtggtgtt
     2341 ggttccgttt taagtttcct accgcaattg tttgtgttat tccttttg
//
LOCUS       DAOSGN010000100         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_43848_length_2376_cov_3.925894, whole genome shotgun sequence.
ACCESSION   DAOSGN010000100 DAOSGN010000000
VERSION     DAOSGN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43848_length_2376_cov_3.925894"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PK087_02000"
     CDS             <1..841
                     /locus_tag="PK087_02000"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOH18076.1"
                     /translation="VYNQKGRYATQQLIKTMCQGEITAVSRLGDLDDLKILDEETMYQ
                     YYKEFIRENLAHIYVVGDFDKNTIMQALQVLYPLKGGDNPYSPIKLSDFIPKQVRQIE
                     EQQKINQARLVMGFRMSIKFLHPFFIPELIFNEMFGGSFSSDLVQTVREKHSLCYSIS
                     SSINVADKIMLVHAGIDGGNSAKVQTLVLEILHRFQKGEFDLNNLQIAKDLMISGLNE
                     IEDRPETMIDFIIKNDLLQRQMTVKEVIDQIKEVSPEQIADVAQGVYLDTVFLLTDEV
                     NHD"
     gene            834..2171
                     /locus_tag="PK087_02005"
     CDS             834..2171
                     /locus_tag="PK087_02005"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOH18077.1"
                     /translation="MIKEYPFFDEQIYQQQLTNGLQLIFIPKNGFKRSFVFFASHYGA
                     AMNRFIPKGEKAYWDAPLGIAHFLEHQLFTMPDGQDASELLAKLGLDSNAFTNYSLTG
                     YLFNGSDNILKGLTLLLDFVQTPHFTEKNVLKEQGIIAQELKMFLDRPGDALHYGLMQ
                     NMFFEYPLKHDVGGTLASIKDITAAHLRTCYRTFYQPANMTIIIVGDLNNIFKQEASF
                     SDLVDFVSEHQKTKKFGKTTEIIVDYPQEPDFVRTNYDEKTMEITVPRAAVGLKIPFA
                     NLDFNQSMLLELRLKILLEANFGTSTDVYQEMLDQALIQSGIATEVYHDGKTGYVKIS
                     ANTEQPQAFVKFVQERLLSLSQFQLDLLTFERFKKALIGNFIRALNSFEFLAVGYLEY
                     QYRNSNLFAALQMSEDLTLEDLSSVQKYFTEDKLTSFIIFPNQSSLSKKASLE"
BASE COUNT          772 a          396 c          450 g          758 t
ORIGIN      
        1 tgtttataac caaaaggggc gctatgcaac gcaacaatta atcaagacga tgtgtcaagg
       61 tgaaattaca gcggttagta gacttggcga tttagacgac ttaaagattt tggacgaaga
      121 aacaatgtac cagtattata aagagtttat tagggagaat cttgcccaca tttatgtcgt
      181 tggtgatttt gataaaaaca caatcatgca agctttacaa gttttatatc ccttaaaagg
      241 tggtgacaat ccttattcac caataaagct tagcgacttt attcccaagc aggttcgaca
      301 aatcgaagaa cagcaaaaga taaaccaagc acgcttggtt atgggatttc ggatgtcaat
      361 caaatttctt catccttttt ttattccgga gttaatattt aacgaaatgt ttgggggaag
      421 tttttcttca gatttggtcc aaaccgtccg cgaaaaacat tcgctttgtt attccatctc
      481 ctcttctatt aatgtagctg acaaaatcat gctcgttcat gccggaattg acggtgggaa
      541 cagcgctaaa gtgcaaaccc ttgttttaga gattcttcat cgttttcaaa agggggagtt
      601 tgaccttaat aacttacaaa ttgcgaaaga tttgatgatt agtggattaa atgagattga
      661 agacagaccg gaaacaatga ttgattttat cattaaaaat gatttattac aaagacagat
      721 gacggttaag gaagtgattg accaaatcaa agaagtaagc ccggagcaaa ttgcggatgt
      781 tgcgcaaggt gtgtatcttg ataccgtctt tttattgact gatgaggtaa atcatgatta
      841 aagaatatcc cttttttgat gaacaaatct atcaacaaca attaactaat ggtttacaac
      901 taatctttat tcccaaaaat ggttttaaac gcagttttgt cttttttgca agccattatg
      961 gagcagcgat gaatcgtttc attcctaaag gcgaaaaggc ctattgggat gccccactag
     1021 gaattgctca ttttttggaa catcaactat ttacaatgcc tgacggtcaa gatgcctccg
     1081 agttattagc caaattaggt ttagatagta atgcttttac aaactattcc ttaaccggtt
     1141 atttgtttaa cggttccgat aatatattaa aaggtctaac tttacttttg gattttgtac
     1201 aaacaccgca ttttacggaa aaaaatgtct taaaagaaca agggattatt gctcaagaac
     1261 tgaaaatgtt tttggatcgt ccgggtgatg cccttcacta tggtttgatg caaaatatgt
     1321 ttttcgaata tcccttaaag catgatgttg ggggaacgct tgcttccata aaggacatca
     1381 ctgccgccca tttacgaact tgttaccgaa ctttttatca accagcaaac atgacgatta
     1441 ttattgttgg tgatctcaat aatattttta agcaggaagc ctctttttcc gatttggttg
     1501 attttgttag tgaacaccaa aaaacgaaaa agtttggaaa aacaacggaa ataattgtcg
     1561 attatccgca agaaccggat tttgtaagaa ctaactatga cgaaaaaaca atggaaatca
     1621 cggttccacg ggcggctgtt ggtttgaaaa taccgtttgc gaatttggac tttaatcaat
     1681 caatgctctt ggaattgcgt ttaaaaattc ttttggaagc gaatttcggt acctcaaccg
     1741 atgtttatca ggaaatgctt gatcaagctt taattcaaag tgggattgcg acagaagtat
     1801 atcatgatgg caagacggga tatgtgaaga taagcgctaa taccgaacaa cctcaagctt
     1861 ttgttaagtt tgtacaagag cgtttattaa gcttatccca atttcaactt gaccttctca
     1921 cttttgaacg tttcaaaaag gcgctgattg gtaatttcat tagagccctt aatagttttg
     1981 aatttttagc agtaggatat ttagaatatc aatatcgtaa tagtaacttg tttgctgctt
     2041 tacaaatgag tgaagacttg accttggagg atttaagctc tgttcaaaag tattttaccg
     2101 aagacaaatt aacgagtttt atcatcttcc ccaatcaatc ctctttatca aaaaaggctt
     2161 ctctagaata aaaaatattt tactttttac taatttaatc agaaataatc aaaaagaaag
     2221 aaacggaaaa atgaataact taattttccg tttttttctc ttcacaaagc aaaatcagtt
     2281 gaaaaaaagt gcagaaggcc gatttcgcgg tttgcactta tttttgctat tggttataat
     2341 acacttgccg gtaaattaaa cgagaggagg atgtgt
//
LOCUS       DAOSGN010000101         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_44011_length_2370_cov_2.418143, whole genome shotgun sequence.
ACCESSION   DAOSGN010000101 DAOSGN010000000
VERSION     DAOSGN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44011_length_2370_cov_2.418143"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PK087_02010"
     CDS             complement(<1..179)
                     /locus_tag="PK087_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HOH18078.1"
                     /translation="MAVKYIPEPFRIKVVENIQILSKQERIQKIADAHYNVFNLNSED
                     VYIDLLTDSGTNAMSA"
     gene            288..1001
                     /locus_tag="PK087_02015"
     CDS             288..1001
                     /locus_tag="PK087_02015"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH18079.1"
                     /translation="MIRTVLFDLDGTLLPLDEELFVQKYFQLLGKKLAKLGFDSQTML
                     AAIWQGTKAMINNNGEVTNEVCFWKTFEKTTKVFKSEIELEFLHFYENEFDLVREASK
                     KQELAPWLVEKLKERGINLILATNPIFPQVATLKRMSWAGLDPQKFSLITTYENSCFA
                     KPNLRYYQQIIQDLNLNSKECLMVGNDVDEDMIANQVGIGTYLITDCLNNRQQVDINL
                     FPNGDFSQFVSYLFKMGII"
     gene            1026..>2370
                     /locus_tag="PK087_02020"
     CDS             1026..>2370
                     /locus_tag="PK087_02020"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOH18080.1"
                     /translation="MKKVILLLLVSFSFLLVSGLQVSGAFDTTVTATGEATYQVIERK
                     AINNLGYGVKQFTDIAQTNRSGSYYNQQVNIMEIPASSPAKIISFANLKSHMWTLSTV
                     ISLATQFEEENPDWRVLGAINADFFDISGTLNGLPYQTHNPVVTNGEFYKTSMRSGVL
                     GFTNDHSTNGLIAGKSNYRSTKMTLDIFDENDNIVNSFSIDKLNQLPETNETSLYFGI
                     YNASSHLYEPKVIDNGNHQTFVVENADLALPNNLSDFYGKGVISSLEPVSLQKGQFAI
                     ATNNSAVQNALGIGKTIRTQFTFINDFARVDSCTGYQGNFLKAGEYFKDTPSVLAARH
                     PRTVVGIRADGTIVMATIDGRQTVKNMDGMYDNEMAATMKRYGCLEAYNLDGGGSTTM
                     IIRKGGEFVVMNSPSDGGLRRDGNCLLIAVKMPVINLSVERSQTELIFNASVEAQN"
BASE COUNT          728 a          400 c          471 g          771 t
ORIGIN      
        1 gcactcatcg cattggttcc actgtcggtt aataaatcga tgtaaacatc ttcagaattt
       61 aaattaaaaa cattataatg ggcatcggca attttttgaa tacgttcctg tttacttaaa
      121 atttgaatat tttcgacgac ttttatccga aaaggttcgg gaatatattt tactgccata
      181 tcaatctcct catttttatt atatcatttt taaaacgggg aaacaatctt aaattaataa
      241 ttgcttttga tgaggaatgt ttagtataat aacagcaggt gatttttttg ataagaacag
      301 ttttatttga tttggatgga acgcttttac ctcttgatga agaattattt gtacaaaaat
      361 attttcaatt attagggaaa aagttggcga agctaggttt tgattcccaa acgatgttag
      421 cagccatttg gcaaggaacg aaagcgatga ttaataataa tggtgaagta acaaacgagg
      481 tatgtttttg gaaaaccttt gaaaaaacaa cgaaagtttt taaatcggaa attgagctgg
      541 aatttctgca cttttacgaa aatgaatttg atcttgttcg tgaagcatcg aaaaagcagg
      601 aattggctcc ttggcttgta gaaaaactca aggaacgggg tatcaatttg attttagcaa
      661 ccaatcccat tttccctcaa gttgcaactt taaaacgaat gtcatgggcg ggcttggatc
      721 cacaaaagtt ttccttaatt acaacttatg aaaattcttg ttttgcaaaa ccgaatcttc
      781 gttattatca acaaattatt caagatttaa atttaaactc taaggagtgt ttaatggttg
      841 gtaatgatgt tgacgaagac atgattgcga accaagttgg aataggtact tatttaatca
      901 cggattgtct taacaatcgt caacaagtcg atattaatct ttttccaaat ggtgattttt
      961 cccaattcgt ttcctatctt ttcaagatgg gcatcatttg atataatatt ttatgtgagg
     1021 tgtatatgaa gaaagttatt ttactcttat tagttagttt tagttttcta ttagtcagtg
     1081 gacttcaagt ttcgggagcc ttcgatacaa ctgtgaccgc aactggagag gcgacttacc
     1141 aagtaattga acgcaaagcc attaacaatt tgggttatgg cgttaaacaa tttactgata
     1201 ttgcgcaaac caaccgctct ggaagttatt acaaccagca agtaaatatt atggaaattc
     1261 cggcatcttc gcccgctaaa attattagtt ttgccaactt aaaatcccat atgtggacat
     1321 tatcgacggt cattagtctt gcaacccagt ttgaggaaga aaacccggat tggcgggttt
     1381 tgggcgccat caatgctgat ttttttgaca ttagcgggac cttaaatggg cttccctacc
     1441 aaacccataa tcccgttgtc actaatggtg aattttataa aacttcgatg aggagtggtg
     1501 ttttgggctt taccaacgat catagtacca atggtttgat tgccgggaaa agcaattatc
     1561 gatccactaa aatgacgctt gatatctttg atgaaaatga taatattgtt aattcttttt
     1621 caattgataa attaaatcaa ctgccggaga ccaatgaaac ttccctctat tttggcattt
     1681 ataatgcttc ttcacatctt tatgaaccga aagttattga taatggcaac catcaaacct
     1741 ttgttgtgga aaatgccgat ttagccctcc ccaacaatct gtcagatttt tatggaaagg
     1801 gagttatttc ttcgttagag ccggtttctt tacaaaaggg gcaatttgct atcgctacca
     1861 ataattctgc tgttcagaat gctttaggga tcggaaaaac cattcgtacc caatttacct
     1921 tcattaatga ttttgcacgg gttgattctt gtactggtta tcaagggaac tttttaaaag
     1981 ccggcgaata ctttaaagat acaccaagcg ttttagcagc acgtcatcct agaacagtgg
     2041 tgggaatccg tgctgatgga acgattgtga tggcgaccat tgatggtcgg caaacagtaa
     2101 aaaatatgga cggtatgtat gataatgaaa tggctgcgac gatgaaacgt tacggttgcc
     2161 tagaagctta caatctcgat ggcggtggct caacaacgat gattatccgt aaaggcggag
     2221 aatttgttgt gatgaactcg ccttcggacg gtggtttacg gcgagatggg aattgtcttt
     2281 tgattgcggt aaaaatgcct gtcattaatc ttagtgttga acgctcgcaa acagaattaa
     2341 ttttcaatgc ctcagttgaa gcccaaaatg
//
LOCUS       DAOSGN010000102         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_44079_length_2367_cov_2.782007, whole genome shotgun sequence.
ACCESSION   DAOSGN010000102 DAOSGN010000000
VERSION     DAOSGN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44079_length_2367_cov_2.782007"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="PK087_02025"
     CDS             <1..1387
                     /locus_tag="PK087_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOH18081.1"
                     /translation="KPHAIMHDAWCDVPHFQATNGVVLNNDFFGGNLQGVIAKLDYLQ
                     SLNVGVIYLNPIFEAFSNHKYDTGDYRKIDSMLGTEADFQLLCAKAKEKGIAIIFDGV
                     YNHTGDDSIYFNKYQRYPELGAYQSRASKYYRWYKFINWPNQYACWWGFKTLPAVNQQ
                     DPYYQQFITGKHGVLDKWLTQGAKGVRLDVIDELDNTFLDKIYAAVKANDPENIVIGE
                     VWEDASTKHAYNQRRRYLWGKQVDSVMNYPLKKAIIDFIKHDNLTGLVIQIRHLINNY
                     PKHVIDSLMNHLGTHDTVRLLTNFAPVSPDQLRKEEQAYYHMSEAEYHEAVRRLKMAT
                     AIQFTLPGVPSIFYGDETGMEGFRDPFCRRTIDWDHINQDLFNWYQKLGKIRHLSVFV
                     DGQYYEESTSNNVFAFSRKKDHEKILTIVNNNHYDISYHLGEGFDLIDELAVSKKVNV
                     PKKTAKIIKLN"
     gene            1498..>2367
                     /locus_tag="PK087_02030"
     CDS             1498..>2367
                     /locus_tag="PK087_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HOH18082.1"
                     /translation="MDNIIERNSASWRWENKTVFHARTVYDCSIDRSAVFNGQGEMLY
                     SQEVVKEGFGSTSLMLKANTDIQDVRPRPACRFSLAINHENWQSFNRLSVWVYPKSQG
                     FINFYFHAAITNKENQNIVHAPSLIPNQWNHVFFEFPDFVREDIIGISMGPLLMGCPP
                     EANPEVAFYFSNIVLEQVSPDYDFGWDLEERIAYCHSGYFLDAEKKALVAASQASSFK
                     LLNEQNEIVFSQPGRLIDNEYGQFWEFDFSTVQKPGLYKIQTGNKETALFPISSFPYR
                     EALWKSVHFLKSLR"
BASE COUNT          705 a          440 c          495 g          727 t
ORIGIN      
        1 aaaaccccat gctatcatgc atgatgcttg gtgtgacgtt ccgcattttc aagccactaa
       61 tggagttgtt ttgaacaatg acttttttgg ggggaattta caaggagtga ttgcgaaact
      121 cgattactta cagtccttga atgtcggtgt gatttattta aaccccattt ttgaagcttt
      181 ttccaatcat aaatatgaca ccggtgatta tcgaaaaatt gattccatgt tgggaacgga
      241 agctgatttc caattgttat gtgccaaagc caaggaaaaa ggcatcgcaa ttatctttga
      301 cggtgtttat aaccacaccg gcgatgacag tatttatttt aataaatacc agcgttatcc
      361 tgaactgggt gcttatcaat cccgagcctc caagtattac cgttggtaca agttcattaa
      421 ctggcctaat caatatgctt gttggtgggg atttaaaacc ctgcccgccg tcaaccaaca
      481 agatccctat tatcaacagt ttattacggg gaagcatggg gttcttgata agtggttaac
      541 tcaaggggcc aaaggcgtgc ggctggatgt tattgatgaa ctggataata cttttttaga
      601 taaaatctat gccgctgtca aagccaatga cccggaaaac atcgtcatcg gtgaagtgtg
      661 ggaagatgcc tccacaaagc atgcttataa tcagcgtcgt cgttatttgt gggggaagca
      721 agtcgacagt gttatgaatt atccccttaa aaaggccatc attgatttta tcaagcatga
      781 caatttaacc ggtttggtca tccaaatccg ccatttaatc aataattatc cgaaacatgt
      841 cattgatagt ttgatgaacc atttaggaac gcatgacact gtccgtttat tgactaattt
      901 tgcccccgtc agtcctgatc agcttcgtaa agaagaacaa gcttattatc acatgagtga
      961 agcggaatat catgaagcgg ttaggagatt gaaaatggca acggccatcc aatttacttt
     1021 gcccggggta ccttctattt tttatggtga tgaaacgggc atggaaggat tccgtgatcc
     1081 tttctgccga agaaccattg attgggatca tatcaatcaa gatctcttta attggtacca
     1141 aaagttaggg aagattcgtc atttatcagt ctttgttgat ggtcaatatt acgaagaatc
     1201 aacgagcaat aatgtttttg ctttttcccg gaaaaaagac cacgaaaaaa tcttaaccat
     1261 cgttaataat aatcattatg acattagcta tcatttggga gaaggctttg atttaattga
     1321 tgagctcgct gtttcgaaaa aagtcaatgt tcctaaaaaa accgccaaaa ttattaaact
     1381 gaactaaacg aatcgttatg attcgttttt ggtttttata aaggtttttt agtaaaaagc
     1441 gtttattaaa agaagaaaat tcagtataat aagggcagga caagaaagga aaaaaccatg
     1501 gacaatatta ttgaaagaaa tagtgccagt tggcgttggg aaaataaaac cgttttccat
     1561 gcgcgcactg tttatgattg cagtattgac agaagtgccg ttttcaacgg tcaaggggaa
     1621 atgctttact cacaagaagt agtgaaagaa ggctttggtt cgaccagctt aatgcttaaa
     1681 gccaataccg atattcaaga tgttcgtccg cgtccagctt gccgtttttc tttggccatt
     1741 aatcatgaaa attggcagag ctttaatcgc ttatcggttt gggtttatcc taaatcccaa
     1801 ggttttatta acttttattt tcatgcggcc attaccaata aggaaaacca aaatatcgtc
     1861 catgctccca gcttgattcc caatcagtgg aatcatgtct tctttgaatt ccctgatttt
     1921 gtgcgggaag atattatcgg aattagcatg ggccccttat tgatgggctg cccgccggaa
     1981 gccaatccgg aagtggcttt ttatttcagc aacattgttt tagaacaagt aagtcctgat
     2041 tatgactttg gttgggattt agaagagcga attgcttatt gccatagcgg ttattttttg
     2101 gatgcggaaa agaaggcttt ggtcgccgct tcccaagcat caagctttaa actattgaat
     2161 gagcaaaatg agattgtctt ctcccaacct gggcgcttaa ttgataatga atatggtcag
     2221 ttttgggaat ttgatttttc aacggtccaa aaaccgggtt tatacaaaat ccaaaccggc
     2281 aacaaagaaa cagccttgtt tcccatctct tcgtttcctt atcgggaagc actttggaaa
     2341 agtgttcact ttttaaagag tttacgc
//
LOCUS       DAOSGN010000103         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_44206_length_2363_cov_3.290728, whole genome shotgun sequence.
ACCESSION   DAOSGN010000103 DAOSGN010000000
VERSION     DAOSGN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44206_length_2363_cov_3.290728"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /gene="rplE"
                     /locus_tag="PK087_02035"
     CDS             <1..122
                     /gene="rplE"
                     /locus_tag="PK087_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388009.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOH18083.1"
                     /translation="INYDKVLKVRGMDIVVVTTAKTDAEGRELLTQMGMPFKK"
     gene            132..317
                     /locus_tag="PK087_02040"
     CDS             132..317
                     /locus_tag="PK087_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOH18084.1"
                     /translation="MAKKSIVFRSKQKQKFAVRQYTRCERCGRPHAVYRKFQLCRICF
                     RELANKGQIPDVTKASW"
     gene            343..741
                     /gene="rpsH"
                     /locus_tag="PK087_02045"
     CDS             343..741
                     /gene="rpsH"
                     /locus_tag="PK087_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOH18085.1"
                     /translation="MVMTDPIADMLTRIRNANQMKHEYVEIPASRIKLEILNVIKREG
                     YIYDVELIPNDKQGVLKVTMKYTEKKQRVIQGLKRISKPGLRLYAKANEMPKVLNGLG
                     MAIVSTSNGVMTDKEARQNNIGGEVIAFVW"
     gene            757..1296
                     /gene="rplF"
                     /locus_tag="PK087_02050"
     CDS             757..1296
                     /gene="rplF"
                     /locus_tag="PK087_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020062304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOH18086.1"
                     /translation="MSRVGNKTIKLAHGTTVTLGANNHVEVKGPKGHIAYDFHPDMII
                     EVKEDSVHVSRPSDSIEHRSLHGTTRALLHNMVEGVTHEFSKTLEIQGTGFRATLKGK
                     VLVLNAGYSHLVEMPIPEGITVVVPTPTEVTVRGVDKQVVGQFATEIYQVRKPEPYLG
                     KGIRFKGQVVRRKEGKKAK"
     gene            1314..1676
                     /gene="rplR"
                     /locus_tag="PK087_02055"
     CDS             1314..1676
                     /gene="rplR"
                     /locus_tag="PK087_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOH18087.1"
                     /translation="MIKKTSRNEMRLARHLRIRKNISGTPTHPRLNVFRSNAHIYAQI
                     IDDVAQKTLVAASSIDKELKLTNGSNIEAARQVGTLIAKRALENKIVTVVFDRGGYLY
                     HGRVKALAEAARTAGLKF"
     gene            1687..2214
                     /gene="rpsE"
                     /locus_tag="PK087_02060"
     CDS             1687..2214
                     /gene="rpsE"
                     /locus_tag="PK087_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOH18088.1"
                     /translation="MRQESRKPKREAFVEVVKEFEERVVSINHVAKVVKGGRRYRFTA
                     VVVVGNKKGRVGLGTGKAIEVPDAIKKAVEDAKKNMIEVPIVNNTIPHEITGVHGAGR
                     VFLKPAKEGTGIIAGGPVRAVVELAGIQDILSKSLGSATPINIVRATVKGLQELRTVE
                     QVSAIRNKTPEEILG"
     gene            2216..>2363
                     /gene="rpmD"
                     /locus_tag="PK087_02065"
     CDS             2216..>2363
                     /gene="rpmD"
                     /locus_tag="PK087_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOH18089.1"
                     /translation="MKTKEIKIKLVKSAIARKPHQSKTLKALGLTKINQIVTKKDNDA
                     IRGMI"
BASE COUNT          781 a          420 c          555 g          607 t
ORIGIN      
        1 aaatcaacta cgataaagtg ttgaaagttc ggggaatgga tattgttgtg gtcaccacgg
       61 ccaaaactga tgctgaaggc cgagaattat taacccaaat gggaatgcct tttaagaagt
      121 aaggaggatt tatggcaaag aaatcaatcg tttttagaag taaacaaaaa caaaaatttg
      181 ccgttaggca atacactcgt tgcgagcgct gtggtcgtcc acatgccgtt tatcgcaaat
      241 ttcaattatg ccgtatttgc tttagagaat tagccaacaa gggacaaatc ccggacgtta
      301 ctaaagcgag ttggtaatag agcttggaag gaggccatct gaatggtaat gactgatccc
      361 attgcggata tgcttactcg cattcgtaat gctaatcaaa tgaaacacga gtatgtggaa
      421 attccggcat caagaattaa gttagagatt ttaaatgtta ttaagcgcga aggttatatt
      481 tatgatgttg aacttatccc taatgataag caaggcgttt taaaagtaac catgaaatat
      541 actgagaaga aacaaagagt aatccaaggt ttgaaaagaa tttccaaacc gggactgcgt
      601 ctttatgcaa aagccaatga aatgcccaag gtcttaaatg gtttagggat ggcaattgta
      661 tcaacatcaa atggcgttat gactgataaa gaagctcgtc aaaacaatat tggcggcgaa
      721 gttatagcgt ttgtttggta attaaggagg attaaaatgt cacgtgttgg taataaaacg
      781 attaaattag ctcatgggac aacggtcact ttgggggcca ataatcatgt tgaagtgaaa
      841 ggcccgaaag gacatatcgc ttatgatttt catccggata tgattattga agtcaaagaa
      901 gattcggttc atgttagccg accgagcgat tccattgagc atcgatccct tcacggaaca
      961 acaagagcac ttttacacaa tatggtagaa ggtgtgaccc acgagttttc gaaaaccttg
     1021 gaaattcaag gaacgggctt cagagcaact ttaaaaggta aagttttagt tttgaatgcc
     1081 ggatactcgc atttagtgga aatgccaatt ccggaaggaa taacagttgt ggtaccaacg
     1141 cccacggaag ttaccgttag aggtgttgat aaacaagttg tcggacaatt cgccaccgaa
     1201 atttatcagg ttcgcaaacc tgaaccatat ctggggaaag gcattcgctt caaaggacaa
     1261 gttgttcgtc gtaaagaagg aaagaaagcg aagtagggag gagacgtaaa caaatgatta
     1321 aaaagacatc aagaaacgag atgcgtttgg ctcgccattt acgcattcgc aagaacattt
     1381 cgggtacgcc gactcaccct cgattaaatg tgttccgctc caatgctcac atttatgccc
     1441 aaattattga tgacgttgct caaaaaacat tggtggcggc atcaagtatt gataaagaat
     1501 taaaattaac caatggttcc aatatcgaag ctgctcgcca agtagggacg ctcattgcga
     1561 aacgagcttt agaaaacaag attgttactg ttgtctttga ccgcggtggc tatctttatc
     1621 acggtcgggt taaagcttta gccgaagcag ctcgtacggc cggcttgaaa ttttaggagg
     1681 tattgaatgc gtcaagaatc aagaaaacca aaaagagaag cgttcgttga agttgttaaa
     1741 gaatttgaag aacgcgttgt cagcatcaat cacgttgcta aagttgttaa aggtggccgt
     1801 cgctatcgtt tcaccgccgt tgttgtcgtc ggaaacaaaa aaggtcgagt cgggttagga
     1861 actggcaaag caattgaagt tccggatgcg attaaaaaag cggtggaaga tgccaagaaa
     1921 aacatgattg aagttcccat tgttaacaat actattcccc atgaaattac cggtgttcat
     1981 ggcgccggtc gcgttttctt aaaaccggcc aaagaaggaa caggcattat tgctgggggt
     2041 ccggtgcggg cggtggtcga attagcgggt attcaagaca ttttatcaaa atcattagga
     2101 tcagcgacgc cgataaatat cgtcagagcg actgtcaaag gtttgcaaga attgcggact
     2161 gttgaacaag taagtgcgat tcgcaacaaa acacctgagg agattttagg ataatatgaa
     2221 gactaaagaa attaaaatca aattagttaa aagcgctatt gctcgcaagc ctcatcaaag
     2281 caaaacttta aaagctcttg gcttaacgaa gatcaaccaa attgttacta aaaaagacaa
     2341 tgatgccatt cgtggcatga ttg
//
LOCUS       DAOSGN010000104         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_44307_length_2359_cov_2.309462, whole genome shotgun sequence.
ACCESSION   DAOSGN010000104 DAOSGN010000000
VERSION     DAOSGN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44307_length_2359_cov_2.309462"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK087_02070"
     CDS             <1..296
                     /locus_tag="PK087_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18090.1"
                     /translation="SFRCLAGKSSYFIRWDGMMMPCAMLEYPSFMAKDDFARAWKKLN
                     LAINNVPLCEGYEDCSVRSFCLQCPARRYLEGGFAQCSPYLKAIATLLKEGKQ"
     gene            293..1156
                     /locus_tag="PK087_02075"
     CDS             293..1156
                     /locus_tag="PK087_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18091.1"
                     /translation="MKTYEIASILLGLDHPFNPLWDEQLAPYETTKAAIYQMTVRFVE
                     EIEVQEEVKDYHSLMKVHEGKDKKVIYTFNKKAVPISRVELSNDLKRITIFLSNKLGD
                     NLFMMEYIVTGLYFLEIALSCGYLPLHASAIIYQNEALLFSAPSGVGKSTQANLWKKN
                     IPEIVFLNDDKPLILCKDQALVFGTPWSGKTFRNVNVSAALKTIVFLKQGPINEIISL
                     SDEDKMIHLFKNTHRSQILINQEVMIESIKHLIGRTKIIAYSCTPNQDAFTTLYLFLY
                     GKEYGTKNNLN"
     gene            1131..1586
                     /locus_tag="PK087_02080"
     CDS             1131..1586
                     /locus_tag="PK087_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HOH18092.1"
                     /translation="MEQRTISIEVIGPLIKEQIDQGKDVIIKVSGHSMMPFYRHQETS
                     VCLEKITKALKRFDVVFYQNFNGQYLLHRIIAIKNQSFIICGDALTKKEYLDKEQLIA
                     KVKYHERKEKITCENSCWYRGFVKMWWFLRPLRFVLIRFFLSKKNKGAK"
     gene            1711..1842
                     /locus_tag="PK087_02085"
     CDS             1711..1842
                     /locus_tag="PK087_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18093.1"
                     /translation="MTEKQVKKEYQAPTIQVVEFTLQEGIAESLLSGENLVCIEELE"
     gene            1848..>2359
                     /locus_tag="PK087_02090"
     CDS             1848..>2359
                     /locus_tag="PK087_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18094.1"
                     /translation="MKKLKFVLFIFVLLTSFLLIGDFFKTQISAVEMSDKSFTVNVTT
                     ILEDGNSVSSTIPNQAFGKKDLTIDLTTPIGQGYQFVFYIVNGKVYRNQNQEFMVTSN
                     LEVVAVLKPTGQNVAVFVDANGALVGFDYVVGTGVPTAPSTASLSKPGYAIDGDQPWS
                     IPVAAISSDT"
BASE COUNT          725 a          397 c          472 g          765 t
ORIGIN      
        1 aaagttttcg ctgtttagcg ggcaaaagtt cctattttat ccgttgggat ggaatgatga
       61 tgccttgtgc gatgttggaa tatccttcgt ttatggctaa agatgatttt gctagggctt
      121 ggaaaaagct taatcttgcc attaataatg ttcctttgtg tgaaggatat gaagattgtt
      181 ccgttcggtc tttttgtctt caatgtcccg ctcggcgcta tttggaaggc ggttttgccc
      241 agtgcagtcc ctaccttaaa gccatcgcca ctttacttaa ggagggaaag caatgaagac
      301 ttacgaaatt gcttccattt tgttggggct tgaccacccc tttaatccgc tttgggatga
      361 acaattagcg ccttatgaaa ctaccaaagc cgctatttac caaatgacgg ttcgttttgt
      421 tgaggagatt gaagtccaag aagaagtgaa ggattatcat tctttgatga aggttcatga
      481 aggaaaagac aagaaagtta tttatacatt taataaaaaa gctgttccca tttccagggt
      541 tgaactaagt aatgatttaa agaggattac tatttttctt tccaacaaac ttggtgacaa
      601 tctttttatg atggaatata ttgtgaccgg attgtatttt ttggaaatag cccttagttg
      661 tgggtatctc cctttacatg cttccgccat catttaccaa aatgaagctt tgttgttttc
      721 tgctccttcc ggtgtcggta aatccaccca agcgaatttg tggaagaaga atattccgga
      781 aattgtcttt ttgaatgacg ataaaccttt aattttatgt aaggaccaag ctctggtttt
      841 cggaacgccg tggagcggaa aaaccttccg gaatgtcaat gtttccgcag ctttaaaaac
      901 gattgtgttt ttaaagcaag gccccattaa tgaaatcatt tctttaagcg atgaagataa
      961 aatgattcac ttatttaaaa atacccaccg cagtcaaatc ctaataaacc aagaagtgat
     1021 gattgaaagc atcaagcact taattggaag gaccaagata atcgcttatt cttgcactcc
     1081 gaaccaagat gctttcacca ccttgtacct ttttttatat ggaaaagaat atggaacaaa
     1141 gaacaatctc aattgaagtt atcggtccct taatcaaaga acaaattgac caagggaagg
     1201 atgtcattat taaagtttcc gggcatagta tgatgccctt ttaccgtcat caggaaactt
     1261 cggtttgttt ggagaaaatt accaaagccc ttaaacgctt tgatgtggtt ttttaccaaa
     1321 actttaatgg tcaatacctc cttcatcgca ttatcgccat taagaatcaa agctttatta
     1381 tttgtggtga tgccttaacc aagaaagaat atttggataa ggagcagctt attgcgaagg
     1441 tcaagtatca tgaacgaaag gaaaaaatca cttgtgaaaa ttcatgttgg tatcgggggt
     1501 ttgtaaaaat gtggtggttt ttacgaccct tgcgttttgt tttaattcgc ttttttctat
     1561 caaaaaagaa caaaggagca aaataaacaa atttgctcct tttgtaaagg ttttgtatat
     1621 tcttttaatc ttggcttgca gtttaagcgt ttgcatgata aaatgaatat aatattgcta
     1681 agaaagcaat aaaataaggt gaggtaaaag atgactgaaa aacaagttaa aaaagagtac
     1741 caagcaccaa caattcaagt ggtggaattt actcttcaag agggcatcgc tgaaagttta
     1801 ttaagcggtg aaaatttagt ctgtatcgaa gaattggagt aaggcaaatg aaaaaattaa
     1861 agtttgtttt atttattttc gttttattaa caagtttttt attaattggt gactttttta
     1921 aaacgcaaat ttcggctgtc gaaatgtcgg ataaaagttt tactgtcaat gtgacgacaa
     1981 tattagaaga cggcaattcc gtttcttcca ccattcctaa tcaagctttt ggaaaaaagg
     2041 atttaacaat tgatttaaca actcctattg ggcaaggtta tcaatttgtt ttctatattg
     2101 ttaatggaaa agtctatcgt aatcaaaacc aagaatttat ggttacatct aatctggaag
     2161 ttgttgctgt tttaaaacca acaggccaaa atgtggcggt ctttgttgat gccaatggcg
     2221 ccttggtcgg ttttgactat gttgttggca caggagttcc aacggcacct tcaacagcca
     2281 gtttatcaaa accgggatat gcgattgatg gtgatcaacc ttggtcaatt ccggttgccg
     2341 ctatttccag tgacaccat
//
LOCUS       DAOSGN010000105         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_44377_length_2356_cov_3.488049, whole genome shotgun sequence.
ACCESSION   DAOSGN010000105 DAOSGN010000000
VERSION     DAOSGN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44377_length_2356_cov_3.488049"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..627
                     /locus_tag="PK087_02095"
     CDS             22..627
                     /locus_tag="PK087_02095"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOH18095.1"
                     /translation="MVQKQIKARGIGDEKVLAAFLQVPRHLFVDPNVANEAYHDHPLG
                     IGLGQTISQPYIVALMTAALQLNKHDRVLEIGTGSGYQTAILSCLVKKVYSIERLSPL
                     IKEAQARLTNLGYHNIVFACQDGNDGWESEAPFDAITIAAATAKIPPKLLNQLKIGGR
                     MILPLGSPFFQRLTLLHKKEDGYDINHLCGCSFVPLISENQ"
     gene            complement(703..1638)
                     /gene="fba"
                     /locus_tag="PK087_02100"
     CDS             complement(703..1638)
                     /gene="fba"
                     /locus_tag="PK087_02100"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOH18096.1"
                     /translation="MPLVNSKEMFKKAYEGGYAIGAFNVNNMEIVQAITEAAKAENSP
                     VILQVSSGARKYAKHVYLVKLVEAALADTDLPIVLHLDHGDSFELCKSCIDGGFTSVM
                     IDGSHLSFEENIALTKKVVDYAHPRNVTVEGELGRLAGIEDDINVKDEHASYTDPKQV
                     QEFVRRTGVDSLAIAIGTSHGAYKFKPGQKPQLRFDILEEIQKLLPGFPIVLHGASSV
                     MPEYVKMINENGGKLEDAIGIPEEMLRKAASMAVCKINIDSDLRLTMTASIRKHFNDH
                     PEHFDPRQYLTDARNNLRNTVQHKIKNVLGSSNRI"
     gene            1877..>2356
                     /locus_tag="PK087_02105"
     CDS             1877..>2356
                     /locus_tag="PK087_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH18097.1"
                     /translation="MQKTAHLAGVLFAIIFSLTFMFSKVAMNYISPMGLIAYRFLLAF
                     LVFEVLRWFKVIKINFNKSQVLILAKISLMEPVLYFIFETFGLAMISSGEAGMMIALI
                     PIFVTILSFIFLKERIRVMQIVFIVISVGGVVFIEWMNKATATNHRLLGVLLVLLASL
                     "
BASE COUNT          666 a          441 c          444 g          805 t
ORIGIN      
        1 cccaattccg cgggctttta tgtggttcaa aagcaaataa aagcccgcgg aattggggat
       61 gaaaaagttt tggctgcctt tttacaggtt ccaaggcatc tttttgttga tccgaacgtg
      121 gctaacgaag cttaccacga ccatccactg ggcattggtc ttggacagac tatttcgcaa
      181 ccctatattg tcgctttgat gacggcagct ttacaattaa ataaacatga tcgcgtttta
      241 gaaatcggca cgggctccgg ttatcaaaca gccattttat cttgcttagt taagaaagtt
      301 tattccattg aacggctttc accgttaata aaagaagccc aagcccgctt aacaaattta
      361 ggttatcata atattgtttt tgcttgccaa gatggtaacg acggctggga aagtgaagcc
      421 ccctttgatg ccattaccat tgccgctgcc acagctaaaa ttcctcccaa actgcttaat
      481 cagctaaaaa ttggcggtcg aatgatttta cccttggggt caccattctt tcagcgttta
      541 acattgttac ataaaaaaga agatggttat gacataaacc acctttgtgg ttgctcattt
      601 gttcctttaa tatcggaaaa tcaatagttt taaataaaaa gaagcaacag agtttgaact
      661 gatatcatat cagtttttgt tgcttctttt aatatactta aattaaatgc ggtttgaact
      721 acctaagaca tttttaattt tgtgttgaac agtatttctt aaattgttac gagcatcagt
      781 caaatattgc cgaggatcga agtgttccgg atgatcgttg aagtgttttc gaatgctggc
      841 ggtcatcgtt aatcgtaaat ccgaatcaat attaattttg cagacagcca tggaagctgc
      901 ttttcgtaac atttcttcgg ggatcccaat ggcatcttcc aattttccgc cgttttcatt
      961 aatcattttt acatattccg gcattaccga agaagccccg tgtaagacga tggggaatcc
     1021 cggtaataat ttttggattt cttccaaaat atcaaatcgt aattgtggct tttgaccggg
     1081 tttaaacttg taagcaccgt gggaagtacc gatggcaatg gctagggaat caactccggt
     1141 tcgtcggaca aattcttgca cttgcttagg atcggtataa ctggcatgtt catccttaac
     1201 attaatatcg tcttcgattc ctgccaagcg tcctaattca ccttcaacgg taacattgcg
     1261 tggatgcgca taatcaacga cttttttggt taaggcgata ttttcttcaa atgataaatg
     1321 ggaaccatca atcatcacgg aagtaaagcc gccatcaata caagatttac ataattcaaa
     1381 actgtcgcca tgatcaagat gcagaacaat cggcaaatcc gtatcagcta aggccgcttc
     1441 cacaagttta actaaataaa catgtttggc atactttctg gctccggatg agacttgtaa
     1501 gataacgggg gaattctctg ctttcgcagc ttcggtaatt gcttggacga tttccatatt
     1561 gttaacattg aaagcgccaa tggcgtatcc accctcataa gcttttttaa acatttcttt
     1621 ggaatttact aatggcataa tttttgctcc tttcttacta tacagtatct tattatagca
     1681 tattttcata aaaattgtta ttaaaaaaga aaaatttatg gaagtaatcc ttttcatgat
     1741 aattagcaat ttcttaatat tgattgttgt tttctttatt caccttcttt gcaaactctt
     1801 attacaataa tgcaataatt gtctttagta aatcttttat ttattgtata attaataatg
     1861 taaaaaggac agtagtatgc agaaaacagc acatttagcg ggcgttctct tcgcgattat
     1921 ctttagctta acttttatgt tttccaaagt agccatgaac tatattagtc cgatgggctt
     1981 gattgcctac cggtttttat tggctttttt ggtttttgaa gtattacggt ggttcaaagt
     2041 catcaagatt aactttaata aatcgcaagt tttaatatta gcgaaaatat ctttgatgga
     2101 acccgtcctt tactttatct ttgagacttt tggcttagcg atgatttcca gtggcgaagc
     2161 cgggatgatg attgctttaa ttcccatttt tgttaccatt ttaagtttta tctttttaaa
     2221 agaacgaatt cgggtgatgc aaattgtttt cattgttatc agtgttgggg gggtggtgtt
     2281 cattgagtgg atgaacaaag ccaccgctac caaccaccgt cttttaggag ttttattggt
     2341 gttattggca tcttta
//
LOCUS       DAOSGN010000106         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_45062_length_2334_cov_2.324704, whole genome shotgun sequence.
ACCESSION   DAOSGN010000106 DAOSGN010000000
VERSION     DAOSGN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45062_length_2334_cov_2.324704"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PK087_02110"
     CDS             <1..1047
                     /locus_tag="PK087_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOH18098.1"
                     /translation="ATGGYFEAVEKGFFIDSGIYPERNGDGIIRKIHGGIGNDSVYER
                     DYDYFAPVTAHFGYNNVEQYDIKAQNNPSSLINGCTFEDRKKIQFIDELDPNDNVDVR
                     MQENAAYRNTSLIKPEVEWLADGYVVVDLTVPVDVKTAAVVGLEIGKKMNLQDVEVIH
                     KEILHPSEATRIQIKGRVPFDINLDDLVIPEDPITLSDQEISDFVKEHPFVIVAGTVG
                     EDEHSVGLREIIDIKHGGIEKYGIMCEYLGTSVPVAKLVDAAIELHADAILMSTIISH
                     DDVHYKNMKKIHEYALEKGVRQQLIIIAGGTQVASDLAKKAGVDEGFGRGTKGAHVAT
                     FLVQKKKEMMKHEN"
     gene            1037..>2334
                     /locus_tag="PK087_02115"
     CDS             1037..>2334
                     /locus_tag="PK087_02115"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOH18099.1"
                     /translation="MKIDALVYEIGSTTTVVNAFNHLDSTPVFLGQAYAPTTVLQGDV
                     RIGIKQALDHLQNQLKTILEPTHSFACSSAAGGLKMSVHGLVYDMTVKAAKEAALGAG
                     ANIRFLTAGPLTKTDVEKIKTLGLNIILIAGGVDYGETQTALFNAQTIAKLQLNIPVI
                     YCGNIANQDAIKTIFTEHHQSQYLFVTENVYPKIDVLQVDEVRTVIQNVFEKHITNAP
                     GMEHVRDAVFGTIIPTPGAVMAAALCLQKELGDLVCIDVGGATTDIHSVCEDNPEHVK
                     MLLNPEPFAKRTVEGDLGVYINKDHLISLIGMAKLQKELQLSESQLLGLLKNYQSIPS
                     EEQIPLVRRLSQEALSQSLNRHAGKYITVYGTSGKSKSIEGKDLTAIKYVIGTGGVLS
                     RIAGGKALMEDVFKNSSEQILLPKTNLQILIDHNYNMATLG"
BASE COUNT          726 a          470 c          496 g          642 t
ORIGIN      
        1 gccaccggtg gctattttga agcggtcgaa aaaggctttt ttattgattc gggaatttat
       61 ccggagcgca atggtgacgg aattattcga aaaatccatg gcggcattgg taatgacagc
      121 gtttatgaac gcgattatga ttattttgca cccgtgacgg cccattttgg ctataacaat
      181 gtcgaacaat atgacatcaa agcccaaaac aatccatcat cactcatcaa cggttgtact
      241 tttgaagacc gaaagaaaat tcaatttatt gatgaacttg accccaacga taatgttgat
      301 gtgcgcatgc aagaaaatgc cgcttaccgt aatacttcct taataaaacc ggaagttgaa
      361 tggctagcgg atggttatgt cgttgtcgat ttaaccgtcc ccgttgatgt gaaaacagcg
      421 gccgtggtcg gtttggaaat tggcaaaaaa atgaatttac aagatgtgga agttattcat
      481 aaagaaatcc ttcatcccag tgaagcaact cgaatccaaa tcaaaggtcg cgttcctttt
      541 gatattaatc ttgatgattt ggtgattccg gaagatccga ttaccttatc cgaccaagaa
      601 atcagtgatt ttgttaagga acaccccttt gtgattgtag ccggaaccgt tggtgaagat
      661 gaacattctg tcggattacg agaaattatc gatatcaaac atggcggtat tgaaaaatat
      721 gggattatgt gtgaatattt aggcacctcg gtcccagtcg caaaattagt tgatgcggcg
      781 attgaacttc atgccgatgc aattttaatg tcaaccatta tctcccatga tgatgttcac
      841 tataaaaaca tgaagaaaat tcatgaatat gctttagaaa aaggcgttcg tcaacaatta
      901 atcatcattg ccggtggaac ccaagttgct tccgatttag ccaaaaaagc gggtgtcgat
      961 gaaggctttg gtcggggaac gaaaggtgca catgtagcaa ctttccttgt tcaaaagaaa
     1021 aaggaaatga tgaagcatga aaattgatgc cctcgtttat gaaattggct caacaacaac
     1081 agtcgttaat gcctttaatc atttagatag caccccggtt tttctcggtc aggcttatgc
     1141 ccccactaca gtcttgcaag gtgatgtccg catcggtatc aagcaagcgc ttgaccattt
     1201 acaaaatcaa ttaaagacta ttttagaacc aacgcatagc tttgcttgtt catcagctgc
     1261 cggtggttta aaaatgagcg tccatggttt agtttatgat atgacggtga aagcggccaa
     1321 agaagccgcc cttggagccg gagccaatat tcgtttttta acggccggtc ctttaaccaa
     1381 gacggatgtg gaaaaaataa aaacgctggg attaaatatt atcctcattg ccggcggtgt
     1441 tgactatggc gaaacccaga cggccttatt caatgcgcaa accatcgcta agttgcaact
     1501 caacatccca gtcatctatt gtggcaatat tgccaaccaa gatgctatta aaaccatttt
     1561 taccgaacat catcaaagcc aatacttatt tgtaaccgaa aatgtctatc ccaaaattga
     1621 tgttttgcag gttgatgaag tgagaacggt aatccaaaat gtttttgaaa aacatattac
     1681 caatgctccc ggcatggaac atgtccgcga tgctgttttc ggaaccatta tccctactcc
     1741 cggtgcagta atggcagcgg ctctttgctt acaaaaagaa ctgggtgatt tagtttgcat
     1801 tgatgtcggg ggggcaacta ccgatattca ttcggtttgt gaagataacc ctgagcatgt
     1861 aaaaatgtta cttaatccgg aaccgtttgc caagagaacg gtagaaggtg atttgggggt
     1921 ctatatcaat aaagaccatc taatatcttt aattgggatg gctaaattgc aaaaagaact
     1981 acagttatcc gaatcacaac ttttaggatt acttaaaaac tatcaatcaa tccccagcga
     2041 agaacaaatt ccccttgtta ggcgcttaag ccaagaagct ttatcacaaa gtttaaaccg
     2101 tcatgccggg aaatatatta ctgtttacgg cacatcgggt aaaagtaaat ccattgaagg
     2161 aaaggactta acagctatca aatatgtcat cggcacgggg ggcgtcctca gtcgaatcgc
     2221 aggtggcaaa gccttgatgg aagatgtttt taaaaacagt tcggagcaaa tcctcctgcc
     2281 aaaaacaaat ctccaaatcc tcattgacca caattacaat atggccactt tggg
//
LOCUS       DAOSGN010000107         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_45181_length_2330_cov_2.724835, whole genome shotgun sequence.
ACCESSION   DAOSGN010000107 DAOSGN010000000
VERSION     DAOSGN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45181_length_2330_cov_2.724835"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PK087_02120"
     CDS             <1..794
                     /locus_tag="PK087_02120"
                     /inference="COORDINATES: protein motif:HMM:NF018902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18100.1"
                     /translation="AQDSFVQNYSSKVKANRKEVKALKLKGTRYQQDWITVVKGSVDV
                     DKLKEVAKKHQMTITEFIATCLNWAASRSLHLFEDKHRPFQLFIPVNMRRFFPSKTLR
                     NFSLYIRTSMFLKDDMTFAEIGDLVKQAFAKQLQKETLHQLMMANVKIEKNIVLRLVP
                     LFLKEIVLKIGYKVWGDSPNSICYSNLGVCDLPQSMHEYIDEVVFTNGASFSTPINIS
                     SISFGKKMVFSVASLLLERNIQRDFFRLLAQFGLDVLIETNELEV"
     gene            797..1474
                     /locus_tag="PK087_02125"
     CDS             797..1474
                     /locus_tag="PK087_02125"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOH18101.1"
                     /translation="MKRCQQCQVNVNTERKSCPLCNEILEDLDQTILPFPVYPKPSQM
                     PKKQNRLIQSLSFLSIMAIIITVVVNVLTFKKHSYYWSALVAVSVFYFWILLKSTFKN
                     KGNIPLKLIVQMLALSATTFAIDYFSDFKGWALNYVIPFLSMAALIAIIFMLIFNFKK
                     INDYLMYLLASNLLGIIPFILWIFRIVEVLWPSLAAAGLSFTVLVGFLVFARKELKDE
                     FKRRFHV"
     gene            1484..>2330
                     /locus_tag="PK087_02130"
     CDS             1484..>2330
                     /locus_tag="PK087_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18102.1"
                     /translation="MSKKTVKKTVKVGKKVKKTSSKAKVWFGLFSFLIALAVGGTGIY
                     YHQVADTENEQVELMIEELKTSLPTQTTNNLTLPNRLSGYPNVAISWVSSNPAVISDD
                     GQVFRPLYATGNKTIRLTATFIIKPDDALAQTFFTTFGISPLQESFTVKVICLPMTNE
                     EKVTQALASLYVPSKTVSDIGLTTQLVEDDAMTITWSSANDTIISATGNVLGLGSTSL
                     TATVTLGSTSEQKTFPIEVVSAYDDIVYTNPNLSEISSGTYANVWNSDNLAFNQAIIA
                     EANNQK"
BASE COUNT          684 a          424 c          446 g          776 t
ORIGIN      
        1 aagctcagga tagttttgta caaaactatt cgtcgaaagt gaaagccaac cgcaaagaag
       61 ttaaagcttt aaaattgaaa ggaacacgct atcaacagga ctggattact gttgttaagg
      121 gcagtgtcga tgttgataaa ttaaaggaag ttgcgaaaaa acaccaaatg acaattacgg
      181 aatttattgc cacttgctta aattgggccg cttcccgttc ccttcattta tttgaagata
      241 agcatcgtcc ttttcaattg tttattcctg ttaacatgcg tcgctttttt ccctcgaaaa
      301 cattacggaa tttctcattg tatattcgga catcgatgtt tttaaaggac gatatgactt
      361 ttgcagaaat tggagatctc gttaaacaag cctttgccaa gcaactgcaa aaagaaactc
      421 ttcatcaatt aatgatggcc aatgtgaaaa ttgaaaagaa tattgttttg cgattagttc
      481 cgctttttct taaggaaatt gtcttaaaaa tcggttataa agtttgggga gattcgccca
      541 acagcatctg ttattccaat ttgggagttt gtgatttgcc gcaatcaatg catgaatata
      601 ttgacgaagt agtttttacg aatggtgctt ctttttccac cccaattaat atttccagta
      661 ttagtttcgg gaagaagatg gttttttcgg ttgccagttt actgctggaa cgcaatattc
      721 aaagggattt ttttcgcttg ttggcgcaat ttggcctcga tgtactaatt gaaaccaacg
      781 agcttgaggt gtgaagatga aaagatgtca gcaatgtcaa gtcaatgtta atacggaaag
      841 aaaatcctgt cccttgtgta atgaaatatt agaagatctt gatcaaacaa tcttgccgtt
      901 tccagtttat ccgaaaccaa gtcaaatgcc gaaaaaacaa aaccgtttaa ttcaaagcct
      961 aagtttttta tcgattatgg ccattatcat caccgttgtt gtcaatgtcc ttacttttaa
     1021 aaaacattcc tattattggt ccgctttggt ggcggtttcc gtgttttatt tttggattct
     1081 tttaaaatcg acttttaaaa acaaaggcaa tattccttta aaattaatcg ttcaaatgtt
     1141 ggctttgtcg gctacgactt ttgccatcga ttattttagt gattttaagg gttgggccct
     1201 taattatgtc attccttttt tgtcaatggc cgctttaatt gccattattt tcatgctgat
     1261 ttttaatttc aaaaaaatca acgattattt gatgtattta ctagcatcca atttgttagg
     1321 aattattcct ttcatcctct ggatttttcg gattgttgag gtgctttggc cgtctttggc
     1381 ggcggccggt ttatctttta cggtcttagt tggtttttta gtgtttgcaa gaaaagagtt
     1441 gaaggacgaa tttaaacgtc gtttccatgt ttaggaggta atcatgagta agaaaacggt
     1501 caaaaagaca gttaaagttg gaaaaaaagt aaagaaaact tcctcaaaag ctaaagtttg
     1561 gtttggtctt ttttcgtttt taatcgcttt agctgtgggt ggaaccggta tttactatca
     1621 tcaggtggct gataccgaaa acgagcaagt tgagttaatg attgaagaat taaaaactag
     1681 tttgcctacg caaacaacga acaatctgac ccttcctaat cgtctttccg gttatccgaa
     1741 tgttgctatt tcgtgggttt ccagcaatcc cgccgtcatt agtgatgacg gtcaagtatt
     1801 tcgacctctt tatgccactg ggaataaaac gattcgctta acagccacct ttatcatcaa
     1861 acccgatgat gctttagcac aaacattttt tacaaccttc gggatttccc cgcttcaaga
     1921 aagctttaca gttaaagtca tttgtttacc gatgactaat gaagaaaaag ttactcaagc
     1981 cttagcttca ttatatgttc cttccaaaac ggtttccgac attggcttaa ctacacaact
     2041 agttgaagat gatgccatga caatcacttg gtcaagtgct aatgatacga ttatctctgc
     2101 aaccgggaat gttttaggct taggctcaac ttctttaaca gcgacagtaa cgctgggcag
     2161 tacaagcgaa cagaaaactt ttccgattga agttgtttcc gcttatgatg acatcgttta
     2221 tacaaacccg aatctttcag aaattagttc cgggacttat gctaatgttt ggaacagcga
     2281 taatcttgct tttaaccaag ccattattgc cgaggccaac aatcaaaaag
//
LOCUS       DAOSGN010000108         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_45861_length_2305_cov_3.350667, whole genome shotgun sequence.
ACCESSION   DAOSGN010000108 DAOSGN010000000
VERSION     DAOSGN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45861_length_2305_cov_3.350667"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PK087_02135"
     CDS             <1..978
                     /locus_tag="PK087_02135"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOH18103.1"
                     /translation="SSFGFTKTGDFVMATIDGRQSLMAGVDLREEAMIMESLGCYQAF
                     NLDGGGSSQLVVRKNNQLIMLNSPSEIYRNNANGLLLVEPDIYVDANITNLRLSGADL
                     NYTLHPSKNIRVISHLLYLNDEALTENDGAVVIEGMVPGGINYLSMTVKYEKQGVIYE
                     RCLWMERINLANYGFVETVIKEKPHNFTVEFTKDESIKGFKAIINCEDPDKTLTKIYL
                     AYDNKQEIAIKRATGYVVDFYNITESRTFTFSVVYYYRLDTINPVFESYEETFTYTYE
                     VETDPIIDPDPKPQPEPNKRGCFSQNIVVSLTMLFFGLLILKRRFGRYD"
     gene            971..>2305
                     /locus_tag="PK087_02140"
     CDS             971..>2305
                     /locus_tag="PK087_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOH18104.1"
                     /translation="MTKIIYIPLDERPCNYRFPVQLFFQANTVVITPAMPIMGYKKRP
                     ADMDALKAWLIKNTGDADGLVVALDTLLYGGIVPSRLHHCSFEDLSQRLAILSELKTQ
                     NPRLIIYAFQLIMRCPQYNSADEEPEYFQTHGKDIHNYGYFHHQQELGMITKEDQAQL
                     KALNIPKEYLSDFSKRRLINQRMNIAALEFVKKGIISFFIFPQDDSSKYGWTAKDQIK
                     IRQAIKDQHLTDLVYMYPGADEAGCVLTSRLVCHLHQISPKVLIKYPGPSCPSIIPSI
                     EDRYLDTMVKYHVLACGGIVVDSLRECDAVLFINAPADYMRSSFSREEPGRGLAVLRN
                     MAEAMAFIDYAYHRQKAIIIADITYGNGSDLEIYQWLAAKNLLDKLAGFGGWNTASNS
                     IGQAIAMGYSYLINGASSAHFQFLASRYVEDIGYCGYVRQFIMADYLPKHPPF"
BASE COUNT          659 a          439 c          500 g          707 t
ORIGIN      
        1 tcttccttcg gctttaccaa aacgggagat tttgtgatgg ccaccattga tggtcgccaa
       61 tctttaatgg ccggtgttga tttgcgtgaa gaagcgatga ttatggaatc attgggttgt
      121 tatcaagcct tcaatttaga tggtggcggt tcaagccaac tggtggtgcg aaaaaacaat
      181 cagttgatta tgcttaattc accgtcggaa atttatcgca ataatgctaa cggtcttttg
      241 ttggtcgaac cggatattta tgttgatgcg aacatcacta atttaagatt aagcggggcg
      301 gatttaaact atactcttca tcccagtaaa aacattcgtg ttattagcca tcttttatat
      361 cttaacgatg aagcgcttac ggaaaatgat ggtgcggtcg ttattgaagg aatggttccc
      421 gggggaatca attatttgtc catgaccgtg aagtatgaaa aacaaggcgt tatttatgag
      481 cgatgcctat ggatggagcg gattaatctc gccaattatg gttttgtgga gacggtgatt
      541 aaagaaaaac cccacaattt tacggtggag tttaccaaag atgaaagtat taaggggttt
      601 aaagcgatta tcaattgtga ggacccggat aaaaccttaa ccaaaatcta tcttgcttat
      661 gataataaac aagaaattgc cattaaaaga gcaaccggtt atgttgttga tttttacaac
      721 atcaccgaat caaggacctt cactttttcc gttgtttatt attatcgtct tgataccatt
      781 aaccctgttt ttgaaagcta tgaagaaact tttacatata catatgaagt tgaaaccgac
      841 ccaataatag atcctgatcc aaaaccacaa cccgaaccga acaaaagagg ttgtttttcc
      901 caaaacattg tcgtttcgct aacgatgtta ttcttcgggc tgctaattct aaaaaggcgc
      961 tttgggagat atgactaaaa ttatctatat acctttggat gaacgaccat gtaattatcg
     1021 ctttccggtt cagctttttt tccaagctaa cacggtggta attacgcccg cgatgcccat
     1081 catgggatat aaaaagcggc cggccgatat ggatgcttta aaagcgtggt taattaagaa
     1141 taccggcgat gccgatggtt tggtggtcgc cttggatacg ctcctttacg ggggcattgt
     1201 gccttcgcga ctgcatcatt gttcttttga agacttatca caaagattgg cgattttatc
     1261 ggaattaaaa acacagaatc cgcgcttaat tatttacgct tttcaattga ttatgcgttg
     1321 cccgcaatat aattctgccg acgaagaacc ggaatatttc caaactcatg gaaaagatat
     1381 tcacaattat ggctatttcc atcatcaaca agaactggga atgattacaa aagaagatca
     1441 agcacagcta aaggcgctta atattcccaa ggaatatcta agcgattttt ccaagcggcg
     1501 tttgattaat caaaggatga atattgcggc tttggaattt gtgaagaaag ggattatttc
     1561 cttttttatc tttccccaag atgattcctc aaaatatggt tggacggcga aggatcaaat
     1621 taaaattcgc caagcgatta aggatcaaca tttaacagat cttgtttata tgtatccggg
     1681 cgccgatgaa gccggctgcg tgcttaccag tcggttagtt tgtcatcttc atcaaataag
     1741 cccgaaagtc ttgattaaat atccgggtcc gagttgtcca agcatcattc cttcgattga
     1801 agaccgttat ttagatacga tggtcaaata tcatgtgttg gcctgcgggg gtatcgtggt
     1861 tgattcctta cgagagtgtg atgccgtttt gttcatcaat gctccggccg attatatgcg
     1921 ctcatctttt tcaagagaag aacccgggcg tggccttgcc gttttaagaa atatggctga
     1981 agcaatggca tttatcgact atgcctacca ccggcaaaaa gcgattatca tcgctgatat
     2041 cacttatggc aacggtagtg atttagaaat ctatcaatgg ttggcggcta aaaatttact
     2101 tgataaatta gcgggttttg gcggttggaa taccgcttcc aatagcattg gtcaagctat
     2161 agccatgggg tattcatatc ttatcaatgg agcaagttcc gcccatttcc agtttctagc
     2221 cagtcgttat gtggaagata tcggctattg cggttatgtc cgtcaattca ttatggcgga
     2281 ttatttacct aagcatccgc cgttt
//
LOCUS       DAOSGN010000109         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_45893_length_2304_cov_3.180969, whole genome shotgun sequence.
ACCESSION   DAOSGN010000109 DAOSGN010000000
VERSION     DAOSGN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45893_length_2304_cov_3.180969"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PK087_02145"
     CDS             <1..300
                     /locus_tag="PK087_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOH18105.1"
                     /translation="IIILIIAKFIQNFMKYEKFQWALQGVKPIVVGMIAAVVVNLVFK
                     NIFGGLFAFSYFDYIALSIMVIIVVLRWRFKKLNPIYLIIISGLLGMLAYTFFPI"
     gene            414..>2304
                     /gene="htpG"
                     /locus_tag="PK087_02150"
     CDS             414..>2304
                     /gene="htpG"
                     /locus_tag="PK087_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOH18106.1"
                     /translation="MSEIKEFKTESKRLLELMINSIYTNREIFLRELISNASDALDKF
                     HYLSLTDQKLEKRDYEIFLEINKKARTLTITDNGIGMTYEELENGLGTIARSGSAEFI
                     KNLKNKSDQETIDIIGQFGVGFYSAFMVASKVEVRTKSPYSDKAYLFKSAGVDTYEVE
                     EVEGLKEGTAVTVFFRKSTKENDFDEFLEEYTIKRLIKKYSDYVRYPIKMNVKKSVPK
                     KDKEGKDIAGKYEDVWEIETLNSMIPLWKKRKKDVTDEELAEFYKQKFYDYQDPQVNI
                     FFNVEGALNYTALIFIPKKAPYNLYSEKYEKGLQLYSKGVFVMEKCKELVPDYLRFVK
                     GLVDSSDLSLNISREILQKNKDLENIAKNVEKKILSKLESMLKNDRDKYIEFFKSFGV
                     NLKFGVYDQFGEKKDLLKDLLLYRSVNQDNYITLKEYVETMPKAQEYIYYASGKTKEQ
                     VLALPQMDLIKKQGFDVLILDEEIDEFAIQMLQEYDKKKFKSINQGDLDLLSKDEEKK
                     IAELSEDKKSMLEKIKEILKDEVSDVILSKRLTDSPVCLVSAEGLSFEMEKVIANMPH
                     EEKPKATKILEINPNHPLFKALENLYNDNNDLLNDYASLLYSQALLIEGFTLKDPVAF
                     SKQMSD"
BASE COUNT          789 a          370 c          441 g          704 t
ORIGIN      
        1 atcattattt tgattattgc taagttcatc caaaacttta tgaagtatga aaagttccag
       61 tgggctttac aaggcgtcaa accgattgtt gtcgggatga ttgctgccgt tgtggtaaat
      121 cttgtgttta aaaatatttt tggtggtctt tttgcttttt catattttga ttatattgcc
      181 ctaagtatca tggtaattat tgttgtttta aggtggcggt ttaaaaagct gaacccgatt
      241 tatttaatca ttatttcagg actcttaggc atgttagcct atactttctt tcccatctaa
      301 ctaagctatt ttccccaata gcttttattt tttcggtctg tttagcactt aatacttgac
      361 agtgctaaaa aaatattgta taatttctaa tagatactaa gaggaggatt tttatgagtg
      421 aaataaaaga atttaaaacc gaatcaaaac ggttgctgga attaatgatt aattcaatct
      481 atacaaaccg agaaattttc ttacgagaat taatttctaa tgcttccgat gccctcgaca
      541 aattccatta tttgtcgtta acggatcaaa aactggaaaa acgggattat gaaattttct
      601 tggaaatcaa caaaaaagcc cggactttaa ccatcaccga taatggtatc ggtatgactt
      661 acgaggaatt ggagaacggc ttaggcacga ttgcgcgaag cggttccgca gaatttatta
      721 agaatctaaa aaacaaaagt gatcaagaaa ctatagacat tatcggtcag tttggggtcg
      781 gcttttattc ggccttcatg gtggcttcca aagtcgaagt gcggacgaaa tcaccctaca
      841 gtgacaaagc ttatttgttt aagtcggccg gcgtcgatac ctatgaagtt gaagaagtcg
      901 aaggtttaaa agaaggaaca gcggtaacgg tgttcttccg caaaagtaca aaagaaaacg
      961 actttgatga attcttggaa gaatatacca ttaaacgctt aatcaagaaa tattccgatt
     1021 atgttcgtta tcccatcaaa atgaacgtga agaaatctgt ccccaaaaaa gacaaagaag
     1081 gtaaggatat agccggcaaa tatgaagatg tttgggaaat cgaaacctta aactcgatga
     1141 ttccgctttg gaaaaagcgc aaaaaagatg tcaccgatga agaattagcc gagttctaca
     1201 aacaaaaatt ctatgactat caagacccgc aagttaatat tttctttaat gtggaaggag
     1261 cccttaatta tacggcttta atcttcattc cgaagaaagc tccctataat ctctattccg
     1321 aaaaatatga aaaaggcttg caactctatt ccaagggtgt tttcgttatg gaaaaatgca
     1381 aggaactggt tccggattac ttaaggtttg tcaagggatt ggtggattcc agtgatttgt
     1441 ctttaaatat cagccgcgag attttacaaa agaacaaaga tttagaaaat attgctaaga
     1501 atgttgagaa gaaaattctc tctaagcttg agagcatgtt gaaaaatgac cgtgacaaat
     1561 acattgaatt ctttaagagt tttggcgtta atttaaaatt cggggtttat gaccaatttg
     1621 gggaaaagaa agacttgtta aaggatttat tgctttaccg ttcggttaac caagataact
     1681 acatcacctt aaaagaatat gtagaaacaa tgcctaaagc ccaagaatat atttattatg
     1741 cttcgggaaa gacgaaggaa caggttttag ccctgcccca aatggattta attaaaaagc
     1801 aaggttttga tgtcttaatt cttgatgaag aaattgatga atttgccatc caaatgttgc
     1861 aagaatatga caagaagaaa tttaagtcca ttaaccaagg cgatttagat ttattatcca
     1921 aagatgaaga aaaaaagatt gcagaattaa gtgaagacaa gaagtcaatg ctggaaaaaa
     1981 tcaaagagat tttaaaagat gaagtttcgg atgtcatttt atccaagcgc ttaactgatt
     2041 ctcctgtttg tttagtcagt gcggaaggct tatcctttga gatggaaaaa gtcattgcca
     2101 atatgccgca tgaagaaaaa ccgaaagcca ccaaaatttt agaaatcaat cctaatcacc
     2161 ccttgtttaa agctttggaa aatctttata acgacaacaa tgacttattg aatgactatg
     2221 cttcgctttt atattcacaa gccttattga ttgaaggctt taccttaaag gaccccgttg
     2281 ctttttccaa acaaatgagt gatt
//
LOCUS       DAOSGN010000110         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_45977_length_2301_cov_3.200801, whole genome shotgun sequence.
ACCESSION   DAOSGN010000110 DAOSGN010000000
VERSION     DAOSGN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45977_length_2301_cov_3.200801"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PK087_02155"
     CDS             complement(<1..599)
                     /locus_tag="PK087_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18107.1"
                     /translation="MEQYLNKGKEVLKILINNGCEAYLIEDVVCNTIMQIPFNEVEII
                     TNATPDMVKGIFSLAKVEPEAEGLVRLFYSGYEFLISTFRKAEKLRDNRLPLRIHYSK
                     DLHDELACRDFTVTAIAMSHAGKLTDAYKGFEDIQRKRIRTIGNPRIRFSEDPVRILK
                     ALRLVSELGFKIHRKTLSGMRSRAKLLAKLPTKALIPEL"
     gene            748..1341
                     /locus_tag="PK087_02160"
     CDS             748..1341
                     /locus_tag="PK087_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOH18108.1"
                     /translation="MKTIEQNVICEQTINKSRFICQLIPVASLAEAKSALSAIKKEHP
                     TATHHCYAYIIGANQSQAKFSDDGEPSNTAGSVIYGVLGKHDLSNILAVVIRYYGGIK
                     LGAGGLVRAYSSSVALALEKAVFLPIINYVILTLTFAYSHHHSVMKLLENYEIIDKVF
                     ESDINLSVRVPKDDVELLSALLSDQSKNQIIIKQNEQ"
     gene            1635..>2301
                     /locus_tag="PK087_02165"
     CDS             1635..>2301
                     /locus_tag="PK087_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH18109.1"
                     /translation="MSVLVCENVTKLYRKHPSVINFNYNFLDNQIYAIIGKSDSGKET
                     LLNLISTKAKPNEGQVFLDGQLLYNNPLMAKRICFISKKMSFPGHLSIREICQLMASF
                     YPKWDNGYAYELMHFFEIKPNTLYYKLFPNKKELLLGILGLASRANITVFENPLDDVD
                     AKDRYDFFNFVYSHHTRYPRTLILSTDYIDEIESIVHKVLFFDKGRLFENFTTEEIKT
                     SFKY"
BASE COUNT          712 a          450 c          407 g          732 t
ORIGIN      
        1 tttaattccg ggattaaagc tttcgtcggt aatttcgcta gtaatttggc tctagaccgc
       61 attccggata atgttttgcg atgaatctta aagcctaatt cactaactaa gcgcaaggct
      121 tttaagatgc gcaccgggtc ttcactgaaa cgaatgcgcg gattaccgat ggtcctaatc
      181 cttttccttt gaatgtcttc aaagccttta taagcatcgg tcagtttacc ggcatggctc
      241 atggcaatgg ctgtaacggt gaaatcgcga caagctaatt catcgtgtaa atccttggaa
      301 taatgaatgc gaagtggcaa acggttgtca cgaagctttt cggctttacg aaaagtcgaa
      361 ataaggaatt catagccgct ataaaataag cggaccagcc cttcggcttc cggctcgact
      421 ttcgcaagcg aaaaaatccc cttaaccata tcgggggtcg cattcgtgat gatttcaact
      481 tcattaaagg gaatctgcat aatcgtatta caaacgacat cctcaataag ataagcttcg
      541 cagccgttgt tgattaatat ttttaaaact tcttttcctt tgtttaaata ttgttccatc
      601 ttaactccag agctccttta tttatcaact ttaattcatt atataataaa accccaaaaa
      661 ttactatgtt tttattgcat tttttatttt tccgaaaaaa accttttcat actcttgcgt
      721 tttcgttata atagatttgg tgatacaatg aaaaccatcg aacaaaatgt catttgtgaa
      781 caaacaatta ataaaagtcg ctttatctgc cagttaattc ctgttgcttc gcttgcggaa
      841 gctaaaagcg ccttgtcagc cattaaaaaa gagcatccaa cagccacgca ccattgttat
      901 gcctacatta tcggtgccaa tcaaagccaa gccaagttct ccgacgacgg cgaaccgagc
      961 aatacggctg gaagcgtcat ttatggagtc cttggcaagc atgatttaag caatattttg
     1021 gccgtagtga tcaggtatta tggcggcatt aaactgggag cgggtggttt ggtccgcgct
     1081 tacagttcca gtgtcgcttt agccctcgaa aaagcggtct ttttgcccat cattaattat
     1141 gtcattctca cactgacttt tgcttattcc catcatcatt cagtgatgaa acttttggaa
     1201 aattacgaaa tcatcgataa ggtttttgaa agcgatatta atctctccgt tcgggttcca
     1261 aaggacgatg ttgagctttt aagcgctttg ttatccgacc aaagcaaaaa ccaaattatt
     1321 attaagcaaa acgagcaata attttaaaaa cagctctcaa accgccttaa aacccgctta
     1381 atttccttca cactacattt tacctttaaa agcgaataaa taaaagtcct aagacaaaac
     1441 gccaaaacaa aagaaaatca gccagcacct aaaaaaagaa atattaaggt gcttttttta
     1501 tttattactt gttttttacg ctttatttat tggaaaaaga ttgttttttg ggaaaaacgg
     1561 aaaggattta attgctttat cggggttttc agtgtatact ttacttatca aaggaataag
     1621 aaaagagcgt tattatgtca gttttagttt gtgagaatgt cacaaaatta tatcgaaaac
     1681 acccttcggt aatcaatttt aactataatt ttcttgacaa ccaaatttat gctattatcg
     1741 gcaaaagcga ttccggaaaa gaaactctac tgaacctaat ttccacaaaa gcgaagccga
     1801 atgaaggtca agtttttttg gatggtcaat tattatataa taatccgctc atggccaaac
     1861 gaatttgctt tatttccaag aaaatgagct tcccggggca tttatcaatc cgggaaatct
     1921 gccaattgat ggcttcgttt tacccaaaat gggataacgg ttatgcttat gaactgatgc
     1981 atttctttga aatcaaacca aatactttat attataaact gttccctaat aaaaaagagt
     2041 tgttgttagg gattttaggc ttggccagcc gtgctaatat cacggttttt gaaaatcctt
     2101 tggacgatgt tgatgcgaaa gaccgctatg atttctttaa tttcgtttat tcccatcata
     2161 ctagataccc tcgcacttta attttatcaa ccgattatat cgatgaaatc gagtcgattg
     2221 tccataaagt cttgtttttt gataaagggc gcctgtttga aaactttacc accgaggaaa
     2281 tcaaaaccag ttttaaatat t
//
LOCUS       DAOSGN010000111         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_46005_length_2300_cov_2.884187, whole genome shotgun sequence.
ACCESSION   DAOSGN010000111 DAOSGN010000000
VERSION     DAOSGN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46005_length_2300_cov_2.884187"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..827
                     /locus_tag="PK087_02170"
     CDS             243..827
                     /locus_tag="PK087_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18110.1"
                     /translation="MIIYLKNNWLFICIILALIGLSLLFILPSGQQIILISSYLAAIY
                     LFLVGISFVVTAFKYSKYLERSQNQKREQILLLFEALFLFALGTVILIFPTYAVRVIV
                     GISLILLPTIHLFTQANKKTYLKNNFWKYLIGILFLVAAESIIDYVFLGLGIIILLGA
                     GFLLYLLIINCRDRNRPNLLVKYGLRYLNKKGRK"
     gene            829..1560
                     /locus_tag="PK087_02175"
     CDS             829..1560
                     /locus_tag="PK087_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOH18111.1"
                     /translation="MGKMELAIIIIVGIFGFLGLFRGFVKQVISMASWLVSLVGAFLL
                     TKRASTWLSASRIGERINTKITAWIATFPVFNVPFEAEHANEQLRDAITELGLPKFIA
                     ELIAKGVDFSTVPNNFTLAEVLSPSITSVFITIITFIALLIMIFIVFKILGHFLNKLF
                     SGKGLGLVNKILGSVLGLLKAALIVCVLMLVISAVAGMIPKVNAWVLADLATPGGLGM
                     AKYIYESNPLVTLIEGSFNFDNLFK"
     gene            1611..>2300
                     /locus_tag="PK087_02180"
     CDS             1611..>2300
                     /locus_tag="PK087_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18112.1"
                     /translation="MIKLENVAKYYHNEGAVTLGLRKINLEFKLGEFVAVTGESGSGK
                     STLLNVISGIDSYEDGEMYIDGEETSYFDDADWEQYRRDAVGFIFQNYNLIDSYTVLK
                     NVETALIIQGMPKKVRLQKAKAIIERVGLTNHLRHRASKLSGGEKQRLSIARALAKET
                     KIIVADEPTGNLDSESSKQVLKLLKEVADGRLVVIVTHDFENVEPYVTRKIRLFDGEV
                     VVDKKLRDYSLT"
BASE COUNT          656 a          367 c          473 g          804 t
ORIGIN      
        1 tgatggtggc ctttgattgc tgcttgcaat ctttttaaag ctttctgaaa atcaaagctc
       61 agtgtttgac cggtttgaaa gaattgactt tgtaaatcaa acaatttatg cataaaatca
      121 ccttttttat cttattatag caaactttta aacatttgtc ataaaagcat ctttgaaaaa
      181 tgatgaaaat aaaactcttt tctttatttt aatatatgtt ataatacggt agggagaaaa
      241 atatgataat ttatttaaaa aataattggc tatttatttg tattatatta gctttaattg
      301 gcctttcttt gttgtttatt ttgccaagtg gccaacaaat tatcttgatt tcttcttatt
      361 tagcagcaat atacttattt ttggtcggaa tttcttttgt tgtgacggca tttaaatatt
      421 ctaaatattt ggaacggtca caaaaccaaa aacgggaaca aattctatta ctatttgaag
      481 ctcttttttt gtttgcgttg ggaacggtga ttttaatttt tccgacttac gcagttcggg
      541 ttattgttgg tatttcatta attcttcttc cgaccattca tttgtttact caagcaaata
      601 aaaaaactta tttaaagaac aatttttgga aatatttaat cggaatatta tttttggtgg
      661 ctgcggaatc gattattgat tatgtatttt taggtttagg tatcatcatt ttgcttgggg
      721 ctgggttttt actttacttg ctaattatta attgtcggga ccgcaatcgt cccaatttgc
      781 ttgtgaaata cggattacgg tatttaaata agaaggggcg gaaataatat ggggaaaatg
      841 gaattagcga ttataattat tgtgggcatt tttgggtttt tgggcttgtt tcgtggtttt
      901 gtgaaacaag tgatttcaat ggcctcttgg ttggtgtcat tagttggtgc tttcttgcta
      961 acaaaaagag ccagcacttg gctaagtgct tctcgcattg gtgagcgcat caataccaaa
     1021 atcactgctt ggattgccac ctttcccgtt tttaatgttc cctttgaagc cgaacatgcg
     1081 aatgagcaat taagggatgc gattactgaa ctgggcttac cgaagtttat tgcggagttg
     1141 attgcgaaag gtgtcgattt ctccaccgtt cctaacaact ttactttggc ggaagttttg
     1201 tctccttcca tcactagcgt tttcattacc attatcacct ttattgcttt gctgattatg
     1261 atctttattg tctttaaaat cctcggtcat ttcctaaaca aactgttttc gggaaagggc
     1321 ttaggacttg ttaacaaaat tttgggctcc gtcctcggct tgcttaaagc cgctttaatt
     1381 gtttgcgtgt tgatgttagt catttctgcg gtcgcaggaa tgattcccaa agttaatgct
     1441 tgggttcttg ccgatttagc gacccccggt ggtttgggga tggcgaaata tatatatgaa
     1501 agcaatccat tagtgacctt gattgaaggc agcttcaatt tcgacaatct atttaaatga
     1561 tgaatatggt cggattctta acccggcctt ttttatagaa atgaggaaat atgattaaat
     1621 tagaaaatgt tgcaaaatat tatcacaatg aaggagcggt gaccttaggt cttcgcaaga
     1681 ttaatttgga atttaaattg ggagagtttg tggcggtgac cggtgaaagc gggagcggta
     1741 aaagtacact cctcaatgtt atcagtggta ttgatagcta tgaagatggc gaaatgtaca
     1801 ttgatggtga agaaacctcc tattttgatg atgctgattg ggaacagtat cgccgggatg
     1861 cagtcggctt tattttccaa aactataatt tgattgacag ttatacggtt ttaaaaaatg
     1921 tggaaacggc tttaatcatt caaggaatgc ccaaaaaagt ccgcttgcaa aaagcgaaag
     1981 ccattattga acgagttggc ctcactaacc atttgcgcca tcgggcctcc aaattatcag
     2041 gtggtgaaaa acaacgctta tcgattgctc gtgctttagc caaagaaacc aaaatcattg
     2101 tagctgatga accgactgga aatttagata gtgaaagtag caaacaggta ttaaaacttt
     2161 taaaagaagt ggccgatggc cgcttagttg taatcgtcac ccatgatttt gaaaatgtcg
     2221 agccatatgt cacacggaaa atccgtttat tcgatggtga agttgttgta gacaaaaaac
     2281 ttcgggatta ttcccttact
//
LOCUS       DAOSGN010000112         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_46609_length_2279_cov_3.258993, whole genome shotgun sequence.
ACCESSION   DAOSGN010000112 DAOSGN010000000
VERSION     DAOSGN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46609_length_2279_cov_3.258993"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PK087_02185"
     CDS             complement(<1..471)
                     /locus_tag="PK087_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH18113.1"
                     /translation="MSKYDELIAQAKTAYQKHELATAIAFYEEAFQEKIIVEDLVDLG
                     FVYLDDKNPFKAVAILEEATAFAFDYPLGFYGLGMAYEEVGRRDDAIKAYQKVIELDE
                     AFVNAYFNLALIYDDLEDENQAYNYYKKTLLYAPGHFWANLNIGSFYEKHDFLDL"
     gene            complement(468..1247)
                     /locus_tag="PK087_02190"
     CDS             complement(468..1247)
                     /locus_tag="PK087_02190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOH18114.1"
                     /translation="MEPLHKKLSNPNFYLIAFLVSFVLFNTLPIVFMISSSLVVEFQQ
                     PGFFIGFLRIISGLDDAAYTPTIVAVLNISQMLGEILLAGLFVAVLWKVLVHDAKLAF
                     KKENIGRNLLIILLGWVAIIVSSVVLELLYERLGIEGTANNQEIIETLLSYDSKIFMI
                     ITVIILAPFVEEILFRKLLFGALEEKFHLSRLWAVIISAVLFAAVHGIDIFFFQYLAL
                     ALVISGAYALSNNNVVVSIGLHFVNNTLSIILFFLAERLLS"
     gene            complement(1219..2139)
                     /locus_tag="PK087_02195"
     CDS             complement(1219..2139)
                     /locus_tag="PK087_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOH18115.1"
                     /translation="MLKDLYVEMTLYQERMHRKSKKKFRDYLHTKAQELNLPYQIVPE
                     SWFSKNVFIGSIDTAEYILSAHYDTPPRMPPFLVKNVITYNIIIMAMIVVLIGLSIIF
                     SIHPFLTSLIIFLFVGYNFGFPAISNKYNLNDNTSGVLTLLYLMQRIKTPKVAFVFFD
                     NEEKGLFGSLGFARYLRKKKLNYQLRKYINFDCVGRGNVFSLDHFNRPQLALRIKSVF
                     DRLQITGYECQVRKARARELSDHFAFIKNSHVGIFALKNHNNKLVINDIHSHRDRQID
                     LNNIFILSKVIETFINQEGGNNGTAPQKTQ"
BASE COUNT          801 a          431 c          416 g          631 t
ORIGIN      
        1 caaatccaaa aaatcatgtt tttcataaaa agaaccaatg tttaaattgg cccaaaaatg
       61 gccgggagca tagagtaaag tttttttata ataattataa gcttgatttt catcttctaa
      121 atcatcataa attaaggcta aattgaaata ggcgttcaca aacgcttcat ccagctcaat
      181 cactttctga taagctttaa tggcatcatc gcgacgccct acttcttcat aagccatccc
      241 taaaccataa aaaccgaggg ggtagtcaaa agcaaaggcc gtggcttctt ccaagatagc
      301 gaccgcttta aaaggatttt tatcatctaa ataaacaaag cctaaatcta ccaaatcctc
      361 cacaatgatt ttttcttgaa aagcttcttc ataaaaagca atggctgtgg ccagttcatg
      421 tttttggtaa gctgttttag cttgggcaat cagttcatca tatttactca tgatagtaat
      481 cgctccgcta agaaaaacaa aataatggat agggtattat tgacaaaatg caagccgatg
      541 gaaacaacaa cattgttgtt ggaaagggca taagcaccgg agattaccaa agctagagcc
      601 aaatattgaa agaagaaaat atcgatacca tggacagccg caaacaacac cgctgaaata
      661 atgaccgccc ataaacggga taaatgaaat ttttcttcca aagcgccgaa caaaagtttt
      721 cgaaagagga tttcctccac aaagggcgct agaataataa cagtaataat cataaaaatt
      781 tttgaatcat aactaagaag agtttcaatt atctcctgat tattcgctgt cccttcaata
      841 cctaagcgtt cataaagaag ctctaaaaca acggaggaaa caataatagc cacccaacct
      901 aataaaataa tcaataagtt tctgccgata ttttctttct tgaaggctaa tttagcatca
      961 tgaaccaata ctttccaaag cacagccaca aaaagcccgg ccaacaatat ttcccccagc
     1021 atttgggaaa tattcaaaac ggcgacaatg gttggtgtat aagccgcatc atcaaggccg
     1081 gaaatgattc gcaaaaaacc aataaagaac cccggctgtt gaaactccac aaccaaagag
     1141 gaagatatca taaaaacaat cggtaaggta ttgaataaga caaaacttac caaaaaagca
     1201 attaaataaa aattaggatt actgagtttt ttgtggagcg gttccattat tccctccttc
     1261 ctgattgata aaagtttcaa taaccttgga tagaatgaaa atattgttta aatcaatttg
     1321 ccgatcccga tgggagtgga tatcattgat tactaattta ttgttatgat tttttaaagc
     1381 gaaaatcccg acatgggagt ttttaataaa ggcgaaatgg tcggataatt cccgtgctcg
     1441 agctttgcga acttggcatt catagcccgt aatctgcaag cgatcaaaaa cacttttaat
     1501 acgcagggct aattgggggc gattaaagtg atcgagtgag aaaacatttc cccggccaac
     1561 acaatcaaaa ttgatgtatt tccgcaactg ataattcagt tttttcttcc gtaaataccg
     1621 agcgaatcct aaggatccaa acaagccttt ttcttcatta tcaaaaaaaa caaaggcaac
     1681 cttgggggtt ttgatgcgtt gcatcaaata aaggagcgtc aacaccccac tggtgttgtc
     1741 gtttaaattg tatttattgg aaatggcagg aaagccgaaa ttataaccga caaaaaggaa
     1801 aataattagt gaagtcaaaa aaggatgaat tgagaaaata atgcttaagc cgattaaaac
     1861 tacaatcata gccataataa tgatgttata agtaatcaca tttttgacta aaaaaggagg
     1921 cattcggggc ggtgtgtcgt agtgcgcact taatatatat tcagccgtat caatgctgcc
     1981 aatgaagaca tttttggaaa accagctttc gggaacaatt tgatagggga gattaagctc
     2041 ttgggctttc gtgtgtaaat agtcacgaaa tttcttttta ctcttgcgat gcatgcgctc
     2101 ttggtaaaga gtcatttcaa catacaaatc tttaagcatc gttattttcc aagcgtccga
     2161 acttcttctt taattaagga aacgaagtca tcaagggaaa ccgttttggt agcgctgtgg
     2221 ccgtattggc ggtaagtaac tagattatcg gcttgttctt gatcacctaa aactaattg
//
LOCUS       DAOSGN010000113         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_46684_length_2276_cov_2.951824, whole genome shotgun sequence.
ACCESSION   DAOSGN010000113 DAOSGN010000000
VERSION     DAOSGN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46684_length_2276_cov_2.951824"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="PK087_02200"
     CDS             <1..1821
                     /locus_tag="PK087_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOH18116.1"
                     /translation="IKKAVDIELEVFVHGALCISCSGHCYLSSIIGKRSGNRGRCAQP
                     CRLKYEFQGGNKYFISPKDLNTLAYLPQLLDAQIDSFKIEGRMKRPEYVAAVVQSYRQ
                     AMDAYQDGSEIDLVQTQENLKKIFNRQFTKGWLLDEKNDNLINEDFSNHIGIEIGQVI
                     KSHKHYVDIKLSHPLNYGDALRIKGQNEDALVVNQMYCEGKIIKQAQAGQIVSLKAHM
                     DGLNQGIVYLTTDQQQIIDLQASFRNQNRQFPIEGVLFLQEQHLMLRLEYKNHSVQVA
                     STEAYQLAKSLSSNERLLSQIQKTGSSPFYFDNLQCTFSENIYLPIKVINELRRSGLE
                     ELTQVVANKHGHRFPNERALPSLPSISLSQGLVAKVRTEQQLHVVAGYPFQEIYVTDE
                     GLMKWQSFYPQHRFLYVIPRINHHQFTDGVFVSSDIGNLNNNHSSIYLPVTNAYSLYH
                     LFSQGVKRVGLSLELSWSQIKQLLTTFQSLFSQKPNLEMMVYGRYELMMMKYCLLKKH
                     GGCEHCDAETTQYLIDRRGFSFPIFKDEQCCVKILNSKRLHLGDYLKSLIENGVTNLL
                     LDFTIENADETKMVCEIYCNNKQEMSLNDVTFGHFKEGVL"
     gene            1818..>2276
                     /locus_tag="PK087_02205"
     CDS             1818..>2276
                     /locus_tag="PK087_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH18117.1"
                     /translation="MNKWYRLDNAAKVFPSVTSRRRSNIFRLSITLKEEVDPNILQTA
                     LEETINRFPSFKVKLKRGFFWFYFEENGEIPKVSEETPYVCEKLNFRAQQRYLFRLTY
                     YHQRISLEIFHALTDGSGAMEFLKAIVYKYLLLKGKPVKPEKIILTDIETR"
BASE COUNT          730 a          397 c          452 g          697 t
ORIGIN      
        1 attaaaaaag ccgttgatat tgaattggaa gtttttgtgc atggggcttt atgtatttct
       61 tgttccggcc attgttattt aagttccatc attggcaaaa gaagtggtaa ccgcgggcgc
      121 tgtgctcaac cctgccgctt aaagtatgaa tttcaggggg gaaacaaata ttttatcagt
      181 ccgaaggact taaacacgct tgcgtattta ccacaattgc tggatgcgca aattgattcc
      241 tttaagattg aaggacggat gaaacgtccg gaatatgtcg ccgccgttgt gcaaagctat
      301 cgccaagcaa tggatgcgta tcaagatgga agcgaaattg accttgttca aacccaagag
      361 aatcttaaaa aaatctttaa ccgccagttt accaaaggtt ggttgttaga tgaaaaaaat
      421 gacaacttaa ttaatgaaga cttttccaat catattggga ttgaaattgg gcaggttatt
      481 aaaagtcata agcattatgt cgatattaag ttatcacatc cgttaaatta tggcgatgcc
      541 cttcgcatta aaggtcaaaa tgaagatgct ctagttgtta atcaaatgta ttgtgaggga
      601 aaaatcatta aacaagccca agccggacaa attgtttcgc ttaaagccca catggatggt
      661 ttaaaccaag gcatagttta tttaacaacg gaccaacaac agattattga tttacaagcc
      721 agttttcgaa accaaaatcg acaatttccg attgaaggcg ttctcttctt gcaagagcag
      781 catttaatgc tccgcttgga atataaaaac cattcggtgc aagtggcctc aacagaagca
      841 taccaattag ctaagagttt atcaagtaat gaacgtttgc taagccaaat ccaaaaaacc
      901 gggtcttcgc cgttttactt tgataattta cagtgtacat tttccgaaaa tatttattta
      961 cccattaaag tcattaacga gttacggcgg agcggtttgg aagaattaac ccaggtggtg
     1021 gcgaacaaac atggccatcg tttccctaat gaacgagcct taccgagcct tccgtcgata
     1081 agtttaagcc aaggtttagt ggccaaagtt cgaaccgaac aacaattaca tgtagtagcg
     1141 ggctatcctt ttcaagaaat ctatgtcacc gatgaaggct taatgaaatg gcagagcttt
     1201 tacccgcagc accgattttt atatgttatt ccgcgcatta accatcacca atttacggat
     1261 ggcgttttcg taagcagtga tattggtaat ttaaacaaca atcatagctc gatttatctt
     1321 ccggttacga atgcttattc tttatatcat ctctttagcc aaggagttaa acgggtcggg
     1381 ctatctttag aactgtcatg gtcgcaaatt aagcaattgc ttaccacctt tcaatcattg
     1441 ttttcacaaa aacccaattt ggaaatgatg gtttatggcc gttatgaatt aatgatgatg
     1501 aaatattgtt tgcttaagaa acatggcgga tgtgaacatt gtgatgctga aaccacccaa
     1561 tatttaatcg atcgtcgtgg tttttcattt cccatcttta aagatgagca atgttgtgtt
     1621 aaaatattaa atagcaagag attgcattta ggggattatc ttaagtcttt gatagaaaac
     1681 ggggtaacga atctattact tgatttcaca atcgaaaatg ccgatgaaac taaaatggtt
     1741 tgtgagattt attgtaacaa caagcaagaa atgtctctca atgatgtgac ttttggacat
     1801 tttaaagaag gagtattatg aataaatggt atcgtttaga taatgccgcc aaagtgtttc
     1861 cctcggttac gagtcggaga aggtcaaata tttttcgttt atcaattacc ttaaaagaag
     1921 aagttgaccc caatatttta cagacggcct tggaggaaac tattaatcgc tttccctctt
     1981 ttaaagtaaa attaaaacga ggtttttttt ggttttactt tgaggaaaat ggtgaaattc
     2041 cgaaagtttc agaggaaaca ccatatgttt gtgaaaaact caatttccgc gctcagcaaa
     2101 gatatttgtt taggttaact tactaccatc aacgcattag cttggaaatt tttcatgctt
     2161 taactgacgg tagtggggcg atggaatttt tgaaggccat tgtctataaa tatttattat
     2221 taaaaggtaa accggttaaa ccagaaaaaa ttattttaac cgatatcgaa acgaga
//
LOCUS       DAOSGN010000114         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_46822_length_2272_cov_2.338295, whole genome shotgun sequence.
ACCESSION   DAOSGN010000114 DAOSGN010000000
VERSION     DAOSGN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46822_length_2272_cov_2.338295"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1494)
                     /locus_tag="PK087_02210"
     CDS             complement(184..1494)
                     /locus_tag="PK087_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOH18118.1"
                     /translation="MKKLADSFQHFMNKKWITTFGRFLRSFINHDLFILFYLVICLIN
                     WKFQLTYLAIVIGVIFLYLIIILDINRFRLVPILLFILASIRLPQPSDYLWSVIIGAV
                     FVLPITLFDLVKKPLRLNNQILIGMIFLLIAMFFSIINTPRLWMSILGVSTMLVYIYI
                     FIYFFNKKQDYSQSELWLYLAKSFTYFGILILLETIFFNFEVVTDNSFISFFNWSVVD
                     LGWANTNYIAMIYLVIIPLTGFWYSKTQKHFYLIIMLLIEIFAFILLISRGAYVAMLV
                     SGLPFFIVFFTDIKNKVAFTQKSLFLISFVLIALLIIAIPTGLVKNFFAILNSRGLSL
                     AGRELLYRIGFNVFLRYPLFGGGIYTSEYYLSLVSTSVYYHNFIIQTLATIGVFGMIA
                     FSYYLYQVFRQSLWKCSYNNYIFIIILGITVHGLFDTTFLTPLL"
     gene            complement(1933..>2272)
                     /gene="rplT"
                     /locus_tag="PK087_02215"
     CDS             complement(1933..>2272)
                     /gene="rplT"
                     /locus_tag="PK087_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOH18119.1"
                     /translation="VVSRRRRKKVLKLAKGYFGSKHTLFRTANAQAMKSLSYAYQDRR
                     DRKSNFRKLWITRINAAARLNGLSYSKLMFGLKGANIEINRKMLSELAINDAVSFTAL
                     CDTAKQKLIK"
BASE COUNT          899 a          352 c          335 g          686 t
ORIGIN      
        1 gttgactttc gcaacataag cagtaatgcc caattgcttg gctagttttt caagggtgat
       61 tttttcttca aaatgaagtt ttttttgatt aattttaatt gtaaacacgg gtaagttcct
      121 ctctattttg ttcatcaatg acatttttct ctaacattaa gggaagaata atggataaaa
      181 tgatcataat aatggggtta aaaaggtggt atcgaacaag ccatgaaccg taattcctag
      241 aataatgata aagatataat tattgtagga acatttccat aaagattggc gaaagacttg
      301 gtaaagataa taactaaaag ctatcatacc gaaaacaccg atggttgcaa gagtttggat
      361 gataaagtta tgataataga cggaggttga gactaaagat aagtaatatt cggaagtata
      421 tataccaccg ccaaagagcg gatatcttaa aaaaacatta aaaccaattc gataaagcaa
      481 ttcgcgacca gccaaagata agccccggct atttaaaatc gcaaaaaaat tcttcacaag
      541 accagtagga atagcgatga ttaataaagc gattaagaca aaactaatga gaaaaaggct
      601 tttttgcgtg aaagcaactt tatttttaat gtcagtaaaa aaaacaataa aaaatggcaa
      661 gccactaacc aacatagcaa cataagcccc ccgagaaatt agcaaaatga atgcgaaaat
      721 ttcgattaaa agcataataa ttaagtaaaa atgtttttgc gttttgctat accaaaaacc
      781 cgtcaaaggg ataatcacta agtatatcat cgcaatatag ttggtattag cccatcctaa
      841 atcaacaaca ctccagttaa agaaactgat aaaactatta tccgttacca cttcgaaatt
      901 aaaaaaaatt gtttccaata aaatcaaaat tccaaaataa gtaaagcttt tagcaagata
      961 taaccacaat tcagattgag aataatcttg ttttttatta aaaaaataaa taaaaatata
     1021 gatatagacc agcatcgtag aaacccccaa gatggacatc cataaacggg gagtattgat
     1081 gattgaaaaa aacatcgcaa ttaacaagaa aatcatcccg attagaatct gattgtttaa
     1141 gcgaagaggt ttttttacta aatcgaagag agtaatcggg agaacaaaca cagcaccaat
     1201 aattaccgac cataaataat cacttggttg tggtaatctt atcgaagcta gaataaacaa
     1261 aagaatcgga acaaggcgaa agcgattaat atctaatata attattaaat aaagaaaaat
     1321 aacaccaata acaatggcta agtaagttaa ttggaatttc cagttaatta aacaaattac
     1381 taaataaaag agaataaata aatcatgatt gataaatgag cgcaaaaatc gcccaaaagt
     1441 agttatccac tttttgttca taaaatgttg aaaactatcg gcaagttttt tcatttaatt
     1501 aattaccttt ttcaagtttt aacagtttta ttatagcata atcatcgaat aatatcaatt
     1561 tatccaccaa taaaaaagaa actctttgcc tttataaact tataaaatta tgtaccatat
     1621 tttcataaca aatggcaaaa cctcaatttg gctatttata gtttttaata tttatttgtt
     1681 ccctttagac ttagaaaatc ccttttaaaa agaatgatta actattcatc taggacatag
     1741 atttataaaa tccataaaga agaaaaaagg ctaattttgt tgcaaaacaa ccgaaaacaa
     1801 ctattaacaa caaatttaag atttttcata atctatttaa aggttttttt ggtattggga
     1861 aactctatcg ataacaaaaa agagactatc atcatattac ctcaactttt aaaaaacacc
     1921 actttcgtga tgttatttta tcagtttttg tttagcggta tcacataatg cagtaaatga
     1981 cacggcatca ttaatcgcta attcggaaag cattttgcgg ttgatttcaa tattggcgcc
     2041 ttttaaacca aacatcaatt tgctataaga aagaccattt aaacgagcag cagcattaat
     2101 tcttgtaatc cacagttttc taaaattgct tttacggtca cgtcgatctt gatatgcata
     2161 agataatgat ttcattgctt gtgcattcgc agtacggaac agggtatgtt ttgagccaaa
     2221 ataacctttg gctaatttta aaactttttt acgtcttctt cgggaaacta ct
//
LOCUS       DAOSGN010000115         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_46863_length_2270_cov_4.098420, whole genome shotgun sequence.
ACCESSION   DAOSGN010000115 DAOSGN010000000
VERSION     DAOSGN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46863_length_2270_cov_4.098420"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PK087_02220"
     CDS             <1..379
                     /locus_tag="PK087_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18120.1"
                     /translation="VKEKIKMLITNMVLFWVALTLILYSLIVLIYVLVKHRTFKLIIL
                     AIVQLIFNVLVTGTLQWSVVLMLSGGRISLFHSTIFPIIGLVGFLIFVGYVVYVTKLI
                     VRRIKVSRTPLKPQTNESPENLK"
     gene            complement(574..1499)
                     /locus_tag="PK087_02225"
                     /pseudo
     CDS             complement(574..1499)
                     /locus_tag="PK087_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
     gene            complement(1492..>2270)
                     /locus_tag="PK087_02230"
     CDS             complement(1492..>2270)
                     /locus_tag="PK087_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOH18121.1"
                     /translation="RLDCDVSLLSVLGDDELGKELLIAASAFMDVTQVDCLKQKSSGC
                     YYAVCNQQGNMELALADMDIDDFLDKTWVLSKTPLMKHYDMIVADLNIQLDGIIELIS
                     WCRSHQQFLVLIGVSGPKMANLPKDLVGIDLIIVNKDETQAYFATSETDLKALALLWK
                     KAGLKRAVITAGGSGLAYFDEHHDVSFLPAKAIENIVDVTGAGDAFSAGVIFGLANHF
                     PFLTALRFGRANASRTIQTETTVRTDLTKTKLLQEEQQDE"
BASE COUNT          664 a          462 c          453 g          691 t
ORIGIN      
        1 agtaaaggag aaaattaaga tgttaataac taacatggta ttattttggg ttgctcttac
       61 tttaatactc tattctttaa ttgttctaat atatgttttg gtaaaacatc gaacatttaa
      121 gttaattatc cttgcgattg ttcaacttat atttaatgtt ttagttaccg gtactttaca
      181 atggtctgtt gttttaatgt taagtggtgg aagaatcagt ctttttcatt caaccatttt
      241 tcccatcatt ggtttagttg gttttcttat ttttgttggc tatgttgtat atgtaacaaa
      301 attaattgtt agaagaataa aagtgagtag aactccattg aaaccacaaa caaatgaaag
      361 tccagagaac ttaaaataaa gactcaatta aataatttag ggctataaat aaaagcattt
      421 cgtcataaaa acgaaatgct ttttttatag ataaactcca agacaattgt cttaatacct
      481 tctttaattt ctaaggttct tcgcttagca aaggacagtt attttaaaca agaacaattg
      541 aacttttgaa acaaatcatc gttaagtgaa acactatttt tgggtttgaa agtaagcaca
      601 agcaatttga gcccctagtt tagcattgtt ataaactaaa actaaattag cggctagact
      661 tttgccctct gttttttcac aatccttttt aataaaaacg gagttacttc ttttcctaag
      721 attccctgct gcttactttc ttcaacagcc gcatcgataa tctgattgat gtaggaggag
      781 tcaagtgaat cggcttttgg tagcggatta acaactaaca acccgccctt taagccaagc
      841 gttgttttag tggcgactac cttagcaatt tcggcgggtg tttcggcggt taaggggacc
      901 gataaatgag aatctctggt ataaaaagcc ggcagaacct tggtttgata gccgataaca
      961 ccgacaccct tggtttctaa atattccatc gtgcgcggta aatccaaaat ggctttggca
     1021 cccgcacaaa tgaccgtaac atcggtatgg gccagttcct ccaaatcggc ggagacatcc
     1081 atggtttctt catagccgcg atggaccccg ccgatgccac cggtaacgaa aaacttaata
     1141 ccggccatgg cggcggcaat cattgtggaa gcgaccgtag ttgcgccgac tttattgaag
     1201 gcaatgacgc tggctaaatc ccggcgtgat actttaatga cttctttagc ccttgccaag
     1261 cgttctaaat cggcttccgt taaaccgatt ttgatttttc cgtccatgat ggcaatggtg
     1321 gccgggacag caccctgatt gcgaataatt tgttcggttt ttttcgccat ttccacattt
     1381 tgaggatagg gcatgccgtg ggaaataatc gttgattcca aagcgacgac ggcttggtga
     1441 tgaagtaagg cttgcttaac ttcctcttta atatctaata agggattaaa attactcatc
     1501 ttgttgttcc tcctgtaata gtttggtttt cgttaaatca gtgcggacgg ttgtttccgt
     1561 ttgaattgtg cggctggcat tggctcgccc gaaacgaagg gcggttaaaa aaggaaagtg
     1621 attggcaaga ccaaaaataa ctccggccga gaaagcatca ccggcgcccg tcacatcaac
     1681 aatattctca attgctttgg caggcaaaaa ggaaacatcg tggtgttcgt cgaaataagc
     1741 caaaccactg ccgccggcag tgatgacggc ccgttttaaa ccggcctttt tccacagtaa
     1801 ggccaaagct tttaggtcgg tttccgatgt tgcgaaatac gcttgggttt catctttgtt
     1861 gacgatgatt aaatcgattc caacaaggtc tttaggcaaa ttagccattt tcggtccgga
     1921 gacaccgatg agaactaaga attgctgatg gcttcgacac caggaaatta gttcaataat
     1981 cccgtctaat tgaatgttta aatccgcaac aatcatatca taatgcttca ttaaaggggt
     2041 tttgcttaac acccaagtct tatcaaggaa atcatcgata tccatatcgg ctagggccaa
     2101 ttccatattt ccctgttgat tgcaaacggc ataataacaa ccggatgatt tttgttttaa
     2161 gcaatcaacc tgcgtcacat ccataaaagc cgaagcggca atcaacagtt cctttcccag
     2221 ttcatcatca cccaaaacgg aaagcaaact gacatcacag tccaaacgtc
//
LOCUS       DAOSGN010000116         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_46943_length_2267_cov_3.268535, whole genome shotgun sequence.
ACCESSION   DAOSGN010000116 DAOSGN010000000
VERSION     DAOSGN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46943_length_2267_cov_3.268535"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PK087_02235"
     CDS             complement(<1..880)
                     /locus_tag="PK087_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18122.1"
                     /translation="MKNSFKLRKLFIFLLFASLAVIVYCAFNRESCGYFFHAGIFVFG
                     ASLTYLLVFFAQNKTIKKLDWMENRLKQWNSISYRVKRAGENSFNEMPLGIIVFNDDK
                     VIEWANNYAKKIFLSPLVDRKIELISQELYANMKRFTEFEITLYGKIFGVSVLTEDNI
                     LYFVDHTDIKMLEQKYRARTLALGIINLDNLSPALAVLDAQEKARQMSNIIGLLSHWV
                     EKYDIYLRGYSEEQYLLIMDNRQLHQIIEDKFKILEEIKSYFLKEDLRITASIGIASA
                     DIVSQKLFEIAYAQLEL"
     gene            complement(985..1209)
                     /gene="rpsR"
                     /locus_tag="PK087_02240"
     CDS             complement(985..1209)
                     /gene="rpsR"
                     /locus_tag="PK087_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOH18123.1"
                     /translation="MANYRMKRKKTCYFTDNKVEKIDWTDYELLKRFVSDRGKILPRR
                     VTGTKAIYQRKLAGAIKRARHMALLPFVRE"
     gene            complement(1224..1682)
                     /gene="ssb"
                     /locus_tag="PK087_02245"
     CDS             complement(1224..1682)
                     /gene="ssb"
                     /locus_tag="PK087_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016093691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH18124.1"
                     /translation="MNKVILVGRLTKDPELRRTNNDIAVVQFTIAVNRTYQSRSGEKQ
                     ADFINCVVWRQQAENLARYMRKGSLIGVEGQIQTRNFDDANGVRRYVTEVICDAIHFL
                     ESKQARQDTSGFSDINQYEIPQANKTQEEPKDPFEDLKSSFDVSNDDLPF"
     gene            complement(1691..1972)
                     /gene="rpsF"
                     /locus_tag="PK087_02250"
     CDS             complement(1691..1972)
                     /gene="rpsF"
                     /locus_tag="PK087_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOH18125.1"
                     /translation="MKQYKGMFIIRPTLAEEAYKQVIADFAKLFEDNESRILEVNEWG
                     MKELAYEIEDFKKGYYVVFVVEATPQAVAEYNRVCNIREDLLRHIIVAE"
BASE COUNT          717 a          446 c          373 g          731 t
ORIGIN      
        1 ctaattccaa ttgggcataa gcgatttcaa agagcttttg cgagacaata tccgccgaag
       61 caatgccgat ggaagcggta atccgtaaat cttcttttag gaaataactc ttgatttctt
      121 ccaatatttt aaatttatct tcaataattt ggtgaagctg ccgattatcc ataatgagta
      181 gatattgttc ttcgctataa ccacgcaaat aaatatcata tttttcaacc caatggctta
      241 acaaaccgat gatgttactc atttggcggg ctttttcctg tgcatcaaga acggctaaag
      301 caggacttag attatcgaga ttgataatac ctaaagccag agtccgagca cgatattttt
      361 gttcgagcat tttgatatcg gtgtgatcga caaaataaag gatgttgtcc tccgtaagga
      421 cgctgacacc aaagattttc ccgtacagtg taatctcaaa ttccgtaaat cttttcatgt
      481 tcgcatataa ttcttggcta atgagttcaa ttttgcgatc aacaagtgga cttaaaaaaa
      541 tctttttggc ataattattc gcccattcaa taactttatc gtcattaaaa acgattatgc
      601 ccagcggcat ttcgttaaag gagttttcac cggcccgttt cactcgatag gaaatggaat
      661 tccactgctt taaacgattt tccatccaat ccagcttttt aatggttttg ttctgagcaa
      721 aaaacacaag gagataggtt aaagaagcgc caaagacaaa aataccggca tggaaaaaat
      781 atccgcatga ctcgcgatta aaagcacagt agacaatcac cgctagggat gcgaaaagta
      841 aaaaaataaa taattttcgc aatttaaaag agtttttcat attatatcac ccttatatac
      901 taataattat atcatagtca atgtaaaaaa taaaatacta tcttccaaaa taaaaaacca
      961 taggttgact atggttttac gaaactactc tcttacaaat ggtaataaag ccatatgacg
     1021 agcccgttta atagcaccgg ctagttttct ttggtaaatt gctttggttc cggtaacccg
     1081 acggggaaga attttcccac gatcggaaac gaatcttttt aataattcgt aatcagtcca
     1141 atcaattttt tcaactttat tatcagtaaa atagcaggtt tttttacgtt tcatacgata
     1201 gtttgccatt tttatcctcc taattaaaat gggagatcat cattggagac atcaaaactg
     1261 ctctttaagt cttcaaatgg atcttttggt tcttcctgag ttttattggc ttgagggatt
     1321 tcatactggt tgatatcact aaacccgctt gtatcctgac gagcttgttt tgactcaaga
     1381 aaatgaatcg catcacagat gacttcggtc acatagcgcc gtacaccgtt ggcatcatca
     1441 aaattacgcg tttgaatttg gccctcaaca ccgattaaac tacccttgcg catgtagcga
     1501 gccaagtttt ccgcttgttg tcgccacacc acgcaattaa taaaatcagc ttgtttttca
     1561 ccacttctac tttggtaagt gcggttaaca gcaatagtaa attgcacaac cgcaatatcg
     1621 ttattggttc ttcttaattc gggatccttg gtcaaacgac caactaaaat aactttgttc
     1681 atagcaactc ctattcagcc acaataatgt gacgaagtag atcttcacga atattgcaaa
     1741 cacggttata ctcagccacg gcttgtggtg ttgcttccac aacaaacaca acatagtagc
     1801 ctttcttaaa gtcttcaatt tcgtaagcta attctttcat tccccattca ttaacttcta
     1861 aaatgcgact ttcgttgtct tcaaacagtt tcgcaaaatc agcgatgact tgtttgtaag
     1921 cttcttcagc taatgttggg cggataatga acataccttt gtattgtttc attttggcac
     1981 ctccttttgg tctaatggct ttttcattac gaaaaagcaa ggatgagctt gctgctcact
     2041 ttgtatatta tactattttt taaagtaaag tcaagaaatt tcccttttta aaaccctaaa
     2101 aattcacata aataataaat aatagtttta tttttctttt aatgatataa tgaagacaca
     2161 agcaggaaaa aagtccaaca aggagaataa tgatgaatta tctggctttt gatatcggtg
     2221 gtacttacac caaatttgca gtcatcaacc ataattatca aatcatt
//
LOCUS       DAOSGN010000117         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_46962_length_2266_cov_4.108548, whole genome shotgun sequence.
ACCESSION   DAOSGN010000117 DAOSGN010000000
VERSION     DAOSGN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46962_length_2266_cov_4.108548"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..295
                     /gene="rpsP"
                     /locus_tag="PK087_02255"
     CDS             29..295
                     /gene="rpsP"
                     /locus_tag="PK087_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOH18126.1"
                     /translation="MAVKLRLQRFGANKRPYYRVVATDSRNPRDGRFIEVVGTYHPIE
                     KNDVLKIDEEKALKWLNNGAQPTDTVKSLLSKLGIIEKFTKQTK"
     gene            303..554
                     /locus_tag="PK087_02260"
     CDS             303..554
                     /locus_tag="PK087_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOH18127.1"
                     /translation="MKEINYEKFLSDLVGPLISFPEEMVVKTFDDDGDVINIQILVNP
                     EDVGRLIGKKGRIINSIRTIAYACAARAGKRIEISVDSF"
     gene            1015..1293
                     /locus_tag="PK087_02265"
     CDS             1015..1293
                     /locus_tag="PK087_02265"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH18128.1"
                     /translation="MEQIEKSVLIFKCLGDITRLNIVMVISKEAKPVGIIANELQMSS
                     SAISHQLRILKNNNIVKSERRGKEVYYQLSDHHIHEMIAQVLNHTSHI"
     gene            1296..>2266
                     /locus_tag="PK087_02270"
     CDS             1296..>2266
                     /locus_tag="PK087_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18129.1"
                     /translation="MEIIKKQFFLDNLENSNYIRKIEFDLNKEFPGSFKLNKETKLLT
                     VDFNENDDIEAKTLQITKIIKAVDAGIDIKPRAIKQTYRRVLILENLDCAICASKVER
                     IAKRTFNYEFIVVDFASTRFIIETTDVDLIAHLEERVQNITQMVDPAIKVVQKTKKKH
                     QFETDFKQDKAGRNFFIIGVSIFVFGFLLKTILKEMTNVKPIIVTFIIYVTYTVGYIM
                     LAWDVLYGAYKNIRSGRIFDEKFLMSLATITALIVGYYDEAVFVMIFYKIGEIFQHHA
                     INYSRKSIAALMDIQPDVAHIIVNGEVVEVDPMEVVVGDMIYVRPGER"
BASE COUNT          761 a          326 c          432 g          747 t
ORIGIN      
        1 ccatcatgtg aaataaggag gtattatcat ggccgtaaaa ctcagattac aaaggttcgg
       61 agctaacaaa cgcccatatt accgtgttgt agcaacggat tcccgcaatc ctcgtgatgg
      121 cagatttatt gaagttgtcg gaacatatca tcccattgaa aaaaatgatg tgctaaagat
      181 tgatgaagaa aaagcattga aatggcttaa caatggcgct caacccactg atacagttaa
      241 atcattgctt tcgaaattag gtattatcga aaaatttact aagcaaacta agtaggtgta
      301 tcatgaaaga aattaattac gaaaaatttc tcagtgactt agttgggccc ctcatttctt
      361 ttcctgaaga aatggttgtg aaaacatttg atgatgatgg tgatgtcatc aacattcaaa
      421 ttttagttaa tccggaagat gttggtcgtc ttatcgggaa aaagggaagg attatcaatt
      481 ctattcgcac aattgcttat gcttgtgccg cccgcgccgg aaaacgaatt gaaatttccg
      541 ttgattcatt ttaaaaagtc aggttttgaa attcatttct aaaagtagat cttatttaaa
      601 aaggtttact ttttttattt tcctcattta aattttttgt ataataatgt tagaaaaata
      661 tgtaaactat aattttaaaa tgatattatt gaagaagata aaaatcatag gagtaatagt
      721 aatagatatt ttagtttaag tcccacaact tgattatgca tatcgtattg tgggatttgt
      781 tttggaaaat ttttgtaaat ggtatcacaa taagaaatct atgcataact aaatggtgtt
      841 tattaaatgg gtaacgaaaa taccattaaa agggtgaaaa aaagacattt gtttttttaa
      901 tatttgtatg tgtttaatgc ggtgtaactg ttttaattat attgattgac ttttatttta
      961 tcctgattta taatatatga agatatgagc aagtgttcaa atgaagaggt agatatggaa
     1021 caaatcgaaa agagtgtttt aatttttaaa tgcctcggcg atattacgcg ccttaacatt
     1081 gtgatggtta tttctaaaga agctaaaccg gttggcatta ttgctaacga attacaaatg
     1141 tcatcatcgg cgatatccca ccaactgcgc attttaaaaa acaataatat tgtgaaaagc
     1201 gaacgaagag gaaaagaagt gtattatcaa ttaagcgatc atcatatcca tgagatgatt
     1261 gctcaagtgc tgaaccacac ttcccacatt taattatgga aataatcaaa aagcagtttt
     1321 ttttagataa tttagaaaat agtaattata tacgcaaaat cgaatttgat ttaaataaag
     1381 aatttcccgg aagctttaag ttaaacaaag aaacaaaatt attgacggtt gattttaatg
     1441 aaaatgacga tattgaagcc aagactttgc aaataacaaa gattattaaa gcggtggatg
     1501 cgggaattga catcaaaccg cgcgccatca aacaaacata tcgccgggtt ctcattttgg
     1561 aaaatctcga ttgtgctatt tgtgcatcaa aagtagagcg gattgcgaaa cggacgttta
     1621 attatgaatt cattgttgtt gattttgctt caacccgctt tattattgaa accaccgatg
     1681 ttgatttaat tgcccattta gaagaacggg ttcaaaatat tacgcaaatg gtcgaccctg
     1741 ccattaaggt tgtgcaaaaa accaagaaga agcatcagtt tgaaacggat ttcaaacagg
     1801 ataaagccgg aagaaacttt tttatcatcg gggtttcaat ctttgttttc ggttttcttt
     1861 taaaaaccat tttaaaagaa atgacgaatg tgaaaccgat tattgtcacc ttcattattt
     1921 atgtgactta taccgttggt tatattatgt tggcttggga tgttttgtat ggagcttata
     1981 aaaacattcg ttccggacgt atttttgatg aaaaattttt aatgtccctg gcaacgatta
     2041 ccgctttaat agttggttat tatgatgaag ctgttttcgt tatgatcttt tataaaatcg
     2101 gggaaatttt ccaacatcac gccattaatt attctcgcaa atcgattgcg gccttaatgg
     2161 acattcaacc ggatgttgct cacattattg tgaatgggga agttgttgaa gtggatccga
     2221 tggaagttgt cgtcggcgat atgatttatg tgcggccggg tgaacg
//
LOCUS       DAOSGN010000118         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_47085_length_2263_cov_2.393569, whole genome shotgun sequence.
ACCESSION   DAOSGN010000118 DAOSGN010000000
VERSION     DAOSGN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47085_length_2263_cov_2.393569"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="PK087_02275"
     CDS             <1..1013
                     /locus_tag="PK087_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOH18130.1"
                     /translation="FIPLIGAIAAGNTAIVKPSLTSLHTSKISKAIIDEAFPNEYVAC
                     LLANHEETNKLLEAHFDHIFFTGSPAMGKMVYEKAAPNLTKVTLELGGKSPCIVDGTI
                     KPQIVARRIAFGKLINAGQTCIAPDYIICKRRALPQLLKELKLAIEKAYPEDPLLTSD
                     YPKIISKRHFDRLIQLLEEQNVEFGGRFNAETLKIEPTVLVTDLNNPLMQEEIFGPVL
                     PIIPYDDERMITSIISHAPNPLAFYVFSKNKAFVKHMLRHHSFGGACVNDTLTHIIPH
                     HLPFGGIKNSGLGNYHGFYSFKTFSQEVAIYHKHPKWELMIRYQPYSSWIKRMIKKFF
                     LK"
     gene            complement(1045..1689)
                     /locus_tag="PK087_02280"
     CDS             complement(1045..1689)
                     /locus_tag="PK087_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18131.1"
                     /translation="MSKYQKRAKNLKLIFLIVGLGVIPLYAIIFSQFGPGGNLILVTF
                     SQIGARYGAIELFIIWGILTSLYYLAFLSYLTVLTRFNNFIMNFLIGFGVISMLVTVF
                     LPFAPSMYPRVSKTHNLLAYVTAVSTLFGLVVFVGSLYRLDLRLFTKSLIALLLVIGI
                     SIIVLLVFGVSSLFQIVFSTLLCVLLCLLLLFIEKSPHINIEQALYDHDQDKEK"
     gene            complement(1682..>2263)
                     /locus_tag="PK087_02285"
     CDS             complement(1682..>2263)
                     /locus_tag="PK087_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18132.1"
                     /translation="SEYGEKLLSIISPTIAFDYPAIIRRLEVAKGLYAKIAAIFLDSS
                     YESLKNELKSLKYSNDDQKKVFMFWEAQHLAWKADRYFLAKLLSQYSFADVVAMIEFE
                     QSLPSTSISPLVAKEMIEILSDLNLHHHCFKIADLAIKGQHLMQLGIPQGKRMKMILS
                     KLLDEIMQGILVNEEAVLLARAKALYQERRKHE"
BASE COUNT          758 a          473 c          395 g          637 t
ORIGIN      
        1 tgttcatccc cttaattggg gcaattgcag ccggcaatac ggcgattgta aaaccgtcat
       61 taacatcttt acataccagt aagattagta aagccattat tgacgaagcc tttcctaacg
      121 aatatgtggc ttgtttatta gcgaatcatg aagaaaccaa caagttactg gaagcccatt
      181 ttgaccatat tttttttacc ggatccccgg ccatgggaaa aatggtttac gaaaaagcgg
      241 cgccaaattt aacgaaagta accctcgaat taggaggaaa aagcccctgt attgttgatg
      301 gcaccatcaa accccaaatc gtcgcccgtc gcattgcttt tggaaaacta atcaatgccg
      361 gtcaaacttg tattgctccg gattacatca tttgcaaacg ccgagccctc ccccagctac
      421 tcaaagaact taagttagcc atcgagaaag cctatcccga agatccgctc ttaactagtg
      481 actatccgaa gataattagc aaacgccatt ttgaccgcct aatacaactc ttggaagagc
      541 aaaatgtgga attcggcggc cgttttaatg cggaaacatt aaaaatcgaa ccgactgtat
      601 tagttaccga cttaaacaat cccttgatgc aagaagaaat tttcggccct gtcctgccca
      661 tcatccctta tgatgatgaa agaatgatta cttccattat ttcccatgcc cccaatcctt
      721 tggctttcta tgttttttcg aaaaataaag cttttgttaa gcatatgctc cgtcatcatt
      781 cctttggagg agcctgtgtc aacgatactt taacacacat tatcccccat catttgccat
      841 ttggaggtat caaaaacagc ggtttgggaa attatcatgg tttttattct tttaaaacct
      901 tttctcaaga agtcgccatt tatcacaaac atccaaagtg ggaactgatg attcgttatc
      961 agccctactc ctcctggata aaaaggatga ttaaaaaatt cttcctaaaa taagcaaaaa
     1021 ggctttttcc gttacgaaaa ggccttattt ttctttatct tgatcatggt cataaagggc
     1081 ttgctcaatg ttgatgtggg gacttttttc gatgaacaat aacaataaac atagtaaaac
     1141 acacaacaaa gtagaaaaaa caatttggaa caaactgcta accccaaaaa ctagcaaaac
     1201 aattatcgaa atccctataa ccaataacaa agcaataagt gatttagtaa agaggcgaag
     1261 atccaagcga tagagcgaac caacaaaaac caccaagcca aagagggtcg agactgcagt
     1321 tacgtaagcc aataaattat gggttttaga aacccgcgga tacatacttg gagcaaaagg
     1381 gagaaacacg gtcacaagca tcgaaatgac tccaaaacca atgagaaaat tcataataaa
     1441 attattgaac cttgtcagca ctgtcaagta agacagaaag gctaaatagt aaagagaagt
     1501 taatattccc caaatgataa ataattcaat agctccataa cgagccccga tttgcgagaa
     1561 agttaccaag attaagtttc caccgggtcc aaactgggaa aaaataatgg cataaagagg
     1621 aataaccccc aaaccgacaa tcaaaaagat taattttaaa tttttggcgc gcttttgata
     1681 tttactcatg ttttcttctt tcttggtaaa gggcttttgc ccgagctaat aataccgctt
     1741 cttcgttcac aagtatccct tgcataattt catctaacag tttggaaaga atcatcttca
     1801 tcctttttcc ttgaggaatg cctaattgca ttaagtgttg acctttaatt gctaaatcag
     1861 caattttaaa acagtgatgg tgaagattga ggtcgcttaa gatctcaatc atttctttag
     1921 caacaagagg actaattgat gttgaaggga gcgattgttc aaattctatc atagccacca
     1981 catcagcaaa agaatattgc gacaataact tcgctaaaaa gtaacgatcc gccttccaag
     2041 ctagatgttg agcctcccaa aacatgaaaa cttttttttg gtcatcattg ctgtatttta
     2101 aactttttaa ttcgtttttt agactttcat aagatgaatc taaaaaaatg gcggcaattt
     2161 tcgcgtataa tccctttgca acttctaaac gcctaattat ggctggataa tcaaaggcga
     2221 ttgtaggtga aattatcgac aagagctttt ccccgtattc aga
//
LOCUS       DAOSGN010000119         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_47387_length_2254_cov_2.365621, whole genome shotgun sequence.
ACCESSION   DAOSGN010000119 DAOSGN010000000
VERSION     DAOSGN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47387_length_2254_cov_2.365621"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="PK087_02290"
     CDS             complement(<1..1240)
                     /locus_tag="PK087_02290"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOH18133.1"
                     /translation="MKKLKIALIGAGSTYSPELIEGLINKKDSLPLGELYLMDIDDRK
                     LKIVGGLCERMIKAAGLDCQVVLSKSLENSLVNADFVLAQIRVGKLPARILDEKIPLK
                     YDLIGQETNGIGGFFKGMRTIPVILDIVRKMEQLCPEAWLINFSNPSGMICEAVLNYT
                     NIKMLGLCNVPINMIDSVKKRLNLPNASVEYVGLNHLTWITAITDQKQDYLQTAIAAG
                     LNSEAMKNIPASGFSADLIRTIKAIPSSYLEYYYFKDAKLQKAKEAKQTRGEVVSEIE
                     EQLLEIYANEALHVKPELLSKRGGARYSEVAINLVDSIYNDKGDIQVVNTLNKGAIPF
                     MADSDAVEISAVIDKNGAHPIPAHFDNEHIKEYMLMMKAYEKHAVKAAISGDEDEAMR
                     ALLINPLVFDYKKAYPCFQEL"
     gene            complement(1255..>2254)
                     /locus_tag="PK087_02295"
     CDS             complement(1255..>2254)
                     /locus_tag="PK087_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOH18134.1"
                     /translation="MIIIILSVAWIWTVFAIIPPAKAMADNPFIVDKGKGPMIAAHRG
                     GKKLNPENTFLAFDDAINRFHIEMLELDLVLTKDNRLVVIHNLSINAVSDVELVTGSN
                     AEYLVGDHSLSELRLFNYGYAFKDKNNNRPYENLVAPLQADRREVIAANRLQIATIDE
                     VFDAYYNLDLLFSVEIKNGGETGKKACDVLNSILNDTKRYPGTDLVNRVVVGTFHDEI
                     EAYLKTTYPKLLRGGSVGEVTKFVLTQMLGVNLLDNSNFVCLQIPTSRKAFNINIKLD
                     KKTYINRAHRRNISVQYWTINDEATMRHLIELGVDVIMTDDPELLYHVLKDMGYRD"
BASE COUNT          657 a          489 c          420 g          688 t
ORIGIN      
        1 tcaactcttg aaagcacgga taagcttttt tataatcaaa cactaaagga ttaatgagaa
       61 gagcgcgcat ggcttcgtct tcatcaccgc taatggcggc tttaacggca tgtttttcat
      121 aagctttcat catcagcata tattctttaa tatgctcatt atcaaagtgg gcaggaatgg
      181 gatgggcgcc gtttttatca ataaccgcac tgatttccac ggcatcgcta tcagccataa
      241 agggaatcgc tcctttgttt agggtgttaa ccacctgaat atcgccttta tcgttataaa
      301 tggaatccac taaattaatt gccacttcgg aataacgagc ccccccacgt ttagacaata
      361 attccggttt cacatgcaaa gcttcattgg catatatttc caacaattgt tcttctattt
      421 cgctgacaac ttcacctcgt gtctgtttgg cttctttggc tttttgcagt ttggcatctt
      481 taaaataata atattctaaa taagacgacg gaatcgcttt gatggtgcga attaagtccg
      541 ccgaaaaacc gctggcagga atgtttttca tcgcttcact gtttaaaccg gccgctattg
      601 ccgtttgtaa ataatcttgt ttttggtcag taatcgccgt aatccaggtt aagtgattta
      661 aaccgacata ttccactgaa gcattgggta aatttaagcg ttttttaacg ctgtcaatca
      721 tattaatggg cacattgcat agtcccaaca tcttgatgtt ggtataattt aagactgctt
      781 cacaaatcat tccggaagga ttggaaaagt taattaacca cgcttccggg cataattgtt
      841 ccattttcct aacaatatcc aaaataacgg ggatggtgcg catcccttta aagaaaccac
      901 cgatgccatt tgtctcctgc ccaattaaat cgtatttaag ggggattttt tcatcgagaa
      961 tgcgggctgg cagttttccc accctaattt gagctaacac aaaatcggcg tttacaagcg
     1021 aattttccaa ggacttgctt aacaccactt ggcaatcaag cccggcagct ttaatcatcc
     1081 gctcacaaag tccaccgaca attttaagct tgcgatcatc aatgtccatt aaatataatt
     1141 cacctaaggg caaagaatcc tttttgttaa ttaagccttc aatcaattcc ggcgaataag
     1201 tgcttcctgc cccaattagg gcaattttta gttttttcat attgttcctc cttgttaatc
     1261 tcgataaccc atatctttta aaacatgata gagtaattcg gggtcatcgg tcataatgac
     1321 atccacccct agttcaatca aatgacgcat agttgcttca tcgttaatag tccaatactg
     1381 aacggaaata ttgcgacggt gggcacggtt aatatatgtt tttttatcta atttaatgtt
     1441 tatattaaag gctttccgac tggtcggaat ctgcaagcaa acaaagttcg aattatccaa
     1501 taaattaaca cccaacattt gggttaaaac aaatttggta acttcgccaa cacttccacc
     1561 tcgtaacagt ttggggtaag tcgtttttaa ataagcttca atttcatcat ggaaggtgcc
     1621 gacgacaaca cgattaacta aatccgtccc gggatagcgc ttagtatcat taaggatgct
     1681 gtttaaaaca tcgcaagcct ttttcccagt ttcgccccca tttttgattt cgaccgaaaa
     1741 caataaatca agattataat aagcatcaaa gacttcgtcg atggtggcga tttgcaagcg
     1801 gttggcagca atcacttccc gccggtcggc ttgaagcgga gcaactaaat tttcataggg
     1861 acggttattg tttttatctt taaaagcata gccgtagtta aacaagcgta attccgataa
     1921 gctatggtcg ccgaccaaat actcggcatt ggaaccggta actaattcaa catcgctcac
     1981 cgcattaatc gataaattat ggatgactac taagcgatta tctttggtca ggactaaatc
     2041 cagttccagc atttcaatgt gaaaccgatt aatcgcatca tcaaaggcga gaaaggtgtt
     2101 ttcgggattg agttttttac caccacggtg ggcggcaatc atcggtcctt tccctttatc
     2161 gacgataaaa ggattatcgg ccattgcttt cgccggagga atgattgcga aaaccgtcca
     2221 aatccaagcc acggataaaa taataattat cata
//
LOCUS       DAOSGN010000120         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_47665_length_2244_cov_3.612608, whole genome shotgun sequence.
ACCESSION   DAOSGN010000120 DAOSGN010000000
VERSION     DAOSGN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47665_length_2244_cov_3.612608"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PK087_02300"
     CDS             <1..1048
                     /locus_tag="PK087_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18135.1"
                     /translation="MALMDPIIVLVSNLAIGTVLLVASYLVSIYTGEARVAMVGTISA
                     YISYLQQIIMSVMMLSMVSVSLSRAAVSANRIQMVLDTKIDIVNNEVALKDIDISGEI
                     EFRNVAFGYVDEDNGVNAKTIENINFKIAAGSTIGVIGSTGSGKTTLVQLIPRLYDVT
                     NGQVLIDGHNVRDYDLNFLREQISYVTQEPLIFSGTINSNIRQGKEDSTEEERIQAAQ
                     LACASEYIEVTSDQYEHAVTQGGTNLSGGQKQRLSLARALVRKPKILILDDSMSAVDA
                     KIEATIKANLKTLNQQTTIIVAQKIASIIHCDNIIVLDNTGHLDGYGKHEQLLSQSRV
                     YQEIYASQFGGANE"
     gene            1041..>2244
                     /locus_tag="PK087_02305"
     CDS             1041..>2244
                     /locus_tag="PK087_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18136.1"
                     /translation="MNRSHSFSRGPRSRFTIVDMPKDKHLQRKTIKRLWQYVKLYPWT
                     LLVVGLVIIFQAAFSLILPYIVRIAIDDYINIETVNLMAVAIIFASVALLTVITAFGS
                     WLQRVLIATATGKITKKIRTDAFLKLQKLPIKYYDQNAHGDIMSVITNDVETIFMALS
                     QVIPQLINAIIMIIGSITLMFFTSWHLTLVVLSLFPLMLLVIAFITTRAFKHFKTQHV
                     KLGEVNGIVKEDIDGLKVVKLYNQEKAMIERFSEANDQLQVASFKAQIYSGMMMPIIR
                     LLDNILYGIVVAVGAVLNIKLGASVISVGKIQSMTNYARMFVRPISNIAQTYNMLQSA
                     VAGGYRVFRLIDEPDEYLNDAPANLPSKQFDVCFEQVNFAYVPKQKVLENISFSIASG
                     KTIAIVGPT"
BASE COUNT          635 a          451 c          470 g          688 t
ORIGIN      
        1 tatggccttg atggatccga ttattgtctt agtcagcaat ctcgctattg gcacggtttt
       61 actagtggcc agttatttag tgtcgattta taccggtgaa gctcgtgttg ccatggtcgg
      121 aaccatttcc gcatacatct cctatctaca acaaattatc atgtccgtca tgatgttatc
      181 gatggtgtcg gtgtcacttt ccagggccgc cgttagtgcc aaccgcattc aaatggtttt
      241 ggatacgaag attgatattg tcaataacga agtcgctctt aaggacatcg atattagcgg
      301 cgagattgaa tttcggaatg ttgctttcgg ttatgtcgat gaagacaacg gtgttaatgc
      361 aaaaacgatt gaaaacatta attttaaaat tgcggccggt tccaccattg gtgtcattgg
      421 ttccaccgga agcggtaaaa caacccttgt tcagctcatc cctcgtttgt atgatgtaac
      481 aaacggtcaa gtcttaattg acggacacaa tgtccgggat tacgatttaa actttttgcg
      541 tgaacaaata tcctatgtta cgcaagaacc acttattttt tccggaacga ttaatagtaa
      601 tatccgccaa ggaaaagaag atagcacgga agaagaacgg attcaagccg cgcaactagc
      661 atgtgcctcc gaatatattg aagtcaccag cgatcaatat gaacatgctg ttacccaagg
      721 aggaaccaat ttgtccggtg gacaaaaaca gcgtttatct ttagcacgag ccttggttcg
      781 taaaccgaaa atcctcattt tggacgattc aatgagcgcc gttgatgcga aaattgaagc
      841 gaccattaaa gctaatttaa aaacattaaa tcaacaaaca acgattattg ttgcccaaaa
      901 aattgcgtcc attatccatt gtgacaatat catcgtttta gataataccg ggcacttgga
      961 cggatacgga aaacacgaac aattattatc ccaaagccgc gtttaccaag aaatttatgc
     1021 ctcgcaattt ggaggcgcaa atgaatagat cgcattcttt ttcgcgagga ccgcggagtc
     1081 gttttacgat tgttgatatg cctaaggata agcatttgca aagaaaaacc atcaaacgcc
     1141 tttggcaata tgtaaaactt tatccttgga ctttgcttgt tgtcggttta gtgattatct
     1201 tccaagcggc tttcagttta atccttcctt acattgtgcg gattgccatt gatgattaca
     1261 ttaatatcga aaccgtcaat ttaatggcag tcgctattat ctttgcttcg gtagccttgt
     1321 taactgttat tacggctttt ggctcgtggt tacaacgcgt ccttattgcg acagcaaccg
     1381 gaaaaattac gaagaaaatc cgcaccgatg cctttttaaa actccagaaa ttaccgatta
     1441 aatactatga ccagaatgct catggcgaca tcatgagtgt cattaccaat gatgttgaaa
     1501 ccatttttat ggctttatcc caagttattc ctcaattaat caacgctatt attatgatta
     1561 tcggttcgat tactttgatg ttctttacaa gctggcattt aactttggtc gttctttctt
     1621 tgtttccgct catgcttttg gttattgcct ttatcacaac ccgggctttt aaacatttta
     1681 aaacccagca tgtgaagctg ggagaagtga acgggattgt gaaagaagat attgatggac
     1741 tcaaggttgt gaaattatat aaccaagaaa aggcgatgat tgagcgtttt agtgaagcca
     1801 atgaccaact gcaagtggcc agtttcaaag cgcaaatcta ttcgggaatg atgatgccca
     1861 tcattcgtct tttggacaac attctttacg gtattgtggt ggctgtcggg gcggtgttaa
     1921 atattaaatt aggagcgagt gttatttcgg ttggtaaaat tcaatcgatg accaattatg
     1981 cgcgcatgtt tgttcgtccc atcagtaata ttgctcaaac atataatatg ctacaatcag
     2041 ccgtagccgg cggttaccgt gtgtttcgtt tgattgatga acccgatgaa taccttaacg
     2101 atgcccccgc caatcttccc agtaagcagt ttgatgtttg ttttgaacag gtcaattttg
     2161 cttatgttcc caagcaaaag gtgttggaga acatcagttt ttccattgca tcggggaaaa
     2221 cgattgccat tgttggaccc accg
//
LOCUS       DAOSGN010000121         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_48892_length_2206_cov_3.313343, whole genome shotgun sequence.
ACCESSION   DAOSGN010000121 DAOSGN010000000
VERSION     DAOSGN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48892_length_2206_cov_3.313343"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PK087_02310"
     CDS             complement(<1..447)
                     /locus_tag="PK087_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18137.1"
                     /translation="MSKIVELLKLIVIPKSMAKVRLMSVLVSLFIFVLSAYLLALPYG
                     YQAAKMVEQYRDNYNFLALQEIPDDDAINQIFVEINNLKCFATPEFTLSCSGLNKGEV
                     FKRDISFVVAGEKYPIPITKHIHFFIENNGDEKSTINPVTDFDVESF"
     gene            complement(511..>2206)
                     /locus_tag="PK087_02315"
     CDS             complement(511..>2206)
                     /locus_tag="PK087_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOH18138.1"
                     /translation="DAQLPLKSVITQKLYNSTLTQSLIAIPMYYEQDLFACAIFTAND
                     STLLNVDAMALLRIACKLLEFKLISFYYQENLRAQKNILQIAMNSLQEGIFYLDQNKQ
                     RMFCTPQLANFLKIEEQMLNRNEFWAWIREEDQSQLLAINEKIDQGEPYEAIYRLRLQ
                     NQEIHVKEQASPYISREGKIKFYVGTIRKIVENIPVSQQKMLMDDELSLQSFLEEVKA
                     KMANLDLKFALARFQIINQDNLSSDLTLQTIKFLYEIIKQEFSNFTFYLADGTFLSYL
                     EIADQRLLDRKIRLVLEKADRGLRYQNFNINFGTKASLVRYPRDSFNVDELLIFSELS
                     LKTPERYQIFSDDIHKKYVKSSQVTFCLQEQLKKGDVALLYLELIAKKESLKSYLVQF
                     NIPGLLPKESLSQFASDFVLVPFQIMALKQLMREIKPEKDFLYYFPISLKALEILHKD
                     QYFITHQSLFLKIRLVINDYAPKLKSLIQHLHQLNMKVLINFHVFKQLNMDDVLENLI
                     YGVLVEENLDYQLRDSLVKISKMFDYELVANFDVLEYTNVKYKSDNLENIDQINAQ"
BASE COUNT          771 a          351 c          342 g          742 t
ORIGIN      
        1 aaatgattcc acatcaaaat ccgtcacagg attaatcgta gatttttcgt ctccattatt
       61 ctcaataaaa aaatgaatat gttttgttat cggaataggg tacttttccc ccgctactac
      121 gaaagaaata tcccgtttga atacttcacc tttatttaaa cccgagcacg acaaagtaaa
      181 ttccggcgtt gcaaaacact ttaaattgtt gatttccaca aaaatctgat taatagcatc
      241 gtcatcgggg atttcttgta acgctaaaaa attataattg tcacggtatt gttcaaccat
      301 tttagcggct tgatagccat agggaagggc taacaaataa gcagataaaa caaaaataaa
      361 taacgaaact aaaaccgaca ttaaccggac ttttgccatc gattttggga taacaattaa
      421 ttttaacaat tcaacaattt tcgacattta ctttcctttt aattcaataa tataaatatt
      481 gtaccataat atattaagaa taaacaatta ttattgggcg ttgatttggt caatattctc
      541 taagttgtca cttttatatt tcacattggt atattccaaa acatcaaaat tggcaactaa
      601 ttcataatcg aacattttac taattttaac taaagaatcc ctcaattgat agtcaagatt
      661 ttcttccact aaaacaccat atattaaatt ttctaaaaca tcatccatat ttaattgctt
      721 aaaaacatgg aagttaatta atactttcat gtttagttga tgtaaatgtt ggattaaaga
      781 ctttagttta ggcgcatagt cattaataac caacctaatt tttagaaata agctttgatg
      841 agtgataaaa tattgatcct tatgtaagat ttcaagcgct tttaaggaaa tcggaaaata
      901 atagagaaaa tctttttccg gtttaatttc gcgcattaat tgttttagag ccatgatttg
      961 aaaagggaca agaacaaaat cagaagcaaa ttgacttaag gattctttgg gtaaaagacc
     1021 aggaatatta aattgaacta aataagattt aagcgattct tttttcgcaa tcaattccaa
     1081 ataaagtaaa gcaacatcac cttttttaag ttgctcttgc aaacaaaaag taacttgtga
     1141 tgatttcaca tattttttat gaatgtcatc agaaaaaatt tgatagcgct caggtgtttt
     1201 taaactcaat tccgagaaaa tcagcagttc atcgacatta aaactatctc gaggatatcg
     1261 aacaagacta gctttagtac cgaaattaat attaaagttt tgatatcgta aaccacggtc
     1321 ggctttctcc aaaactaaac ggattttacg atcaagaagc ctttgatcgg cgatttctag
     1381 gtaggaaaga aaagttccat cagctaaata gaaagtaaaa ttcgaaaatt cctgtttaat
     1441 aatttcatac aaaaatttga ttgtttgcaa agttaaatcc gaactaagat tatcttgatt
     1501 gatgatttga aagcgagcta gcgcgaattt taagtctaaa ttcgccattt tagctttcac
     1561 ttcctccaaa aatgattgta aagaaagttc atcgtccata agcatttttt gttgtgaaac
     1621 aggaatattc tcaacaattt tgcgaattgt tccgacatag aatttgattt taccctcccg
     1681 agaaatataa ggagatgctt gttctttcac atggatttcc tgattttgta aacgaaggcg
     1741 gtatattgct tcatatggtt cgccttgatc aattttttca ttgattgcta acaactgact
     1801 ttggtcttct tctctaatcc aagcccaaaa ttcattccga tttaacattt gctcctcaat
     1861 ttttaaaaag ttggcaagtt gaggtgtgca aaacatccgc tgtttgtttt gatcaagata
     1921 aaaaattcct tcttgtaaag aattcatagc gatttgtaag atattttttt gagctcgcaa
     1981 attttcttga tagtaaaaag agatgagttt aaattccaat aatttacaag caattcgtaa
     2041 taaagccatg gcatcgacat ttaaaagcgt tgaatcattg gcagtaaaaa tggcacaggc
     2101 aaacaaatct tgttcataat acatgggaat tgcaatcaat gattgggtta gtgtggaatt
     2161 ataaagtttt tgggtgatga cactttttaa tggtaactgg gcatcg
//
LOCUS       DAOSGN010000122         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_49070_length_2201_cov_3.356477, whole genome shotgun sequence.
ACCESSION   DAOSGN010000122 DAOSGN010000000
VERSION     DAOSGN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49070_length_2201_cov_3.356477"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PK087_02320"
     CDS             <1..436
                     /locus_tag="PK087_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HOH18139.1"
                     /translation="KSPAKYIYYGLAFLSLAFGVYLIAKNEAYFGIFCLFLAVIIIVV
                     RLATAHTTLNKIMKDLRFPTFKYQLVFSESSLSLIQGDVKTSYPWNKIIVICETKTMM
                     YFYTSASQALILGKYLLSDQERHQVQQWLSLKQVKHRFYRLK"
     gene            450..665
                     /locus_tag="PK087_02325"
     CDS             450..665
                     /locus_tag="PK087_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18140.1"
                     /translation="MKIILISLIIILGVAIGLILGLGLFQGFELVLSMFILFPLVFIL
                     GTILLGRTKKEVPKEKDHQQDRFSRWE"
     gene            665..1423
                     /locus_tag="PK087_02330"
     CDS             665..1423
                     /locus_tag="PK087_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18141.1"
                     /translation="MSDLRLFNLKKANLETKTKLDKHALQAIISAHGEVLLGITVIAN
                     DLNITDNPTEKVETLGYDENFRLTIIEYRSGKFGGVVSKGLMFLDYIANNQGKIKSLV
                     NEKLGYDKGKQINYAPRLVIIGEDFNKYDEYAIKQMPYIVELLKYQLFEKQTLVLEKI
                     YQSKNTYGEAKLSSDLINQIRDFTLSLGDEVCEEISTSFICFRKIKAFAYLLIQDGLT
                     LSILQKGKYKQYQVRQQKDIAKVEDLIEQSYDEN"
     gene            1398..2114
                     /locus_tag="PK087_02335"
     CDS             1398..2114
                     /locus_tag="PK087_02335"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH18142.1"
                     /translation="MNKAMTKTEDLPVIYEDNHLIVVLKQPNLLSQADHTQDDDMINL
                     VKQYLKNKYQKQGNVFVGLVQRLDRRVGGLMVFAKTSKAAARMSALIRERSFQKEYLA
                     IVQGKTPPSGRLVDYLCKIYKDGPRAIVVSAEDFGQEAILEYESLATALIDQEEFSFV
                     KINLITGRYNQIRIQFAHFQHPLINDYKYGYRGHHESDELGLVCSGLKFVHPVTKETM
                     SFRYRPSEGLWKYFGSVNYE"
BASE COUNT          704 a          356 c          445 g          696 t
ORIGIN      
        1 aaaaagcccg gccaaatata tctattatgg tttagctttt ttgagcttgg catttggtgt
       61 ttatctaatt gcgaaaaacg aagcttactt tggcattttt tgtttatttc tggccgtcat
      121 tatcattgtg gtgcggttgg caacggctca tacaaccctt aacaaaatca tgaaggattt
      181 acgctttccg acctttaaat atcagttagt tttttccgag tcatcattat cattaattca
      241 aggtgatgtg aaaacaagct atccttggaa taaaatcatt gtcatttgtg aaaccaaaac
      301 gatgatgtat ttttatacat cagcttcaca agcgcttatt ttaggaaaat atctattatc
      361 cgaccaagag cgtcatcaag tgcaacaatg gctaagcctt aaacaagtaa aacatcgctt
      421 ttatcggctt aaataaacgg tgcgtactta tgaaaatcat tttaatcagt ttaatcatta
      481 tcctaggagt ggcaattggt ttaattttag gattgggttt atttcaaggt tttgagttgg
      541 ttttgtcaat gtttattttg tttccgttag tctttatttt gggaacgatt ttactgggga
      601 gaaccaagaa agaagttccc aaagaaaaag accatcagca agaccgtttt tcgaggtggg
      661 aataatgagt gatttacgct tattcaactt aaagaaagct aatttagaaa caaaaacaaa
      721 actggataaa cacgctttac aagccatcat cagcgctcat ggggaagtgt tgctggggat
      781 taccgtcatt gctaatgatt taaacattac cgataatccg acggaaaaag tggaaacact
      841 aggctatgat gaaaacttcc gcttaacaat cattgaatat cgcagtggta aatttggtgg
      901 tgttgttagc aaaggcttga tgtttttaga ttatattgcc aataatcaag gtaagataaa
      961 aagtttggtc aatgaaaaac ttggttatga caaaggaaag caaatcaatt atgctccgcg
     1021 cttagtgatt atcggtgaag actttaataa atatgatgaa tatgccatca aacaaatgcc
     1081 gtatattgtg gaactgctca aatatcaatt gtttgaaaaa caaaccttag tgttggaaaa
     1141 aatctaccaa agtaaaaata cttacggtga agccaagcta agttcggatt taatcaatca
     1201 aatccgtgat tttaccctca gtttaggtga tgaagtttgt gaggaaatta gcacttcgtt
     1261 catctgtttt cgaaaaatta aagcttttgc ctatttgttg atacaagatg gcttaacttt
     1321 aagcatcttg caaaagggca aatataagca atatcaagtc cgccagcaaa aagatatagc
     1381 aaaggtggaa gatttaattg aacaaagcta tgacgaaaac tgaggattta ccagttattt
     1441 atgaagacaa tcatctcatt gtcgtgttga aacagcctaa tttattatct caggccgatc
     1501 atacgcagga tgatgatatg attaatcttg ttaaacaata tttaaaaaat aaatatcaaa
     1561 aacaaggaaa tgtttttgtt ggcctagtgc agcgtttgga tcggcgtgtg ggcggattaa
     1621 tggtgtttgc gaaaacatcg aaagcagcag cacgcatgtc cgctttgatc cgggaacgaa
     1681 gttttcaaaa agagtacttg gccattgttc aggggaaaac cccaccctcg ggacggttag
     1741 tcgattatct ttgcaagata tataaagatg gtccccgggc cattgttgta agtgcggaag
     1801 attttggcca agaagccatt ttggaatatg aaagcttagc gacggccctt attgatcaag
     1861 aagaattttc ttttgtgaaa atcaatttaa ttaccggacg ttataatcaa attcggattc
     1921 agtttgctca tttccaacat ccgcttatta atgattataa atatggttat cgcggacatc
     1981 acgaaagcga cgaattggga ctggtgtgtt cgggtttaaa atttgtccat cccgtcacta
     2041 aggaaaccat gagttttcgt tatcgtccgt ccgagggtct ttggaaatat tttgggagtg
     2101 tgaattatga ataaagctaa acctgcgatt attatttttg ctacctttat cgtgctattt
     2161 attttaatgt cgctttttgc ggctaacgga gaaatcaaag t
//
LOCUS       DAOSGN010000123         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_49227_length_2196_cov_3.296123, whole genome shotgun sequence.
ACCESSION   DAOSGN010000123 DAOSGN010000000
VERSION     DAOSGN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49227_length_2196_cov_3.296123"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /locus_tag="PK087_02340"
     CDS             <1..1613
                     /locus_tag="PK087_02340"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOH18143.1"
                     /translation="KTLVETMPNKQRRVLWGISDFGFNYHLQPQESFVSCEAVMSYSN
                     QGLNQLSQHFHDFVNHHIIPKEFSFKNRPVVLNNWEATYFNFNERKIIDLAKKARDVG
                     IELFVLDDGWFGKRNDDRSSLGDWDVNLKKLPRGLKGLADKITAMGMKFGLWVEPEML
                     NEDSELYRKHPNWAIKFPKAKPSTGRNQMVLDLTNPDVRTFLKTKLREILNSATISYI
                     KWDYNRNITDMYGTTLSNQSEFMHRYILGLYDVLQYLTTTFPQVLFEGCASGGNRFDL
                     GMLCFFPQIWTSDNTDYVERLAIQTGTSYGYPLSVISNHVSTVPNHQTLRTTPLSSRF
                     NLACFGVLGYELNLCEEDQNTVKMIKTQIAFYKQYRHLWQFGRMYRSEPTIFNHNRSG
                     LYVMDHQQNLGIYGFFQILVHPDMSSDVVKINHLSDDYYVFYNLPQKLNVLDFGGLIN
                     IVSPIHIKVNGKIHRLLSKIYRPSAEKERYVVHGKAFFAAGVKLNPQFMGTGFNKEVR
                     VIGDFGSRLYIFTKINHKYRLKRTKLMN"
     gene            complement(1629..>2196)
                     /locus_tag="PK087_02345"
     CDS             complement(1629..>2196)
                     /locus_tag="PK087_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOH18144.1"
                     /translation="KIVAEKALHAYVFNSLPPYDVLENKYLTRKDLAEIKLVEEGLNR
                     YYNSHRFPKTLDYLLKDCQLNPYQLFLTLGTYLSLHPECRLQIKDLAKAFYKAITTKN
                     QDYFLFLLKQDYLDNAKLKPPLWWDYELTKSQTKAVLDLFKQQYPQLSKQNLMHNSRL
                     ITWSNKETNHYYLVLYQPKQSYTLNKKD"
BASE COUNT          675 a          372 c          440 g          709 t
ORIGIN      
        1 acaaaacgct tgtggaaaca atgccgaaca aacaacgacg agtattatgg ggcattagtg
       61 atttcggctt taattatcat cttcaaccac aagagtcatt tgtttcttgt gaagcggtta
      121 tgagctatag caatcagggt ttaaaccaat tgtcgcagca ttttcatgac tttgttaatc
      181 atcatattat tcctaaagaa ttcagtttta aaaatcgtcc cgttgtttta aacaactggg
      241 aagcaaccta ttttaatttt aatgaacgta aaatcatcga cttggctaaa aaagctcgtg
      301 atgtcggaat tgaattattt gtgcttgatg acggttggtt tgggaagcgc aatgatgatc
      361 gttcatcatt aggtgattgg gatgtcaatc ttaagaaact gccccgcggt ttaaaaggtc
      421 tagccgataa aatcacggcc atgggcatga aatttggttt atgggtggaa ccggagatgc
      481 ttaacgaaga tagcgagcta tatcgaaagc atccaaattg ggcgattaaa tttcctaagg
      541 caaaaccgag caccggccgc aatcaaatgg tgttggactt aacgaatccc gatgttagaa
      601 cttttttaaa aacgaaacta agggaaattt taaattctgc gactatcagt tatatcaaat
      661 gggactataa ccggaatatc acggatatgt acgggacgac tttaagcaac caaagcgaat
      721 tcatgcatcg ttatatcttg ggtttatatg atgttttgca gtatttaacg acaactttcc
      781 cgcaggtttt gtttgaaggt tgtgccagcg gcgggaaccg ctttgattta ggaatgcttt
      841 gtttctttcc ccaaatttgg acaagcgata ataccgatta tgtggaacgc ttggccattc
      901 aaacagggac ttcctatggg tatccgcttt ccgtcatctc caatcatgtc agcactgtcc
      961 ccaaccatca aaccttaaga acgacgccgc tatcttcacg gtttaacctg gcttgttttg
     1021 gggttttggg atatgaactc aatctttgtg aggaagatca aaacaccgtc aaaatgatta
     1081 aaacgcaaat tgctttttat aaacaatatc gccatttatg gcaatttggg aggatgtatc
     1141 gcagcgaacc gacaattttt aatcataacc gtagcggctt atatgtaatg gaccaccaac
     1201 aaaatttagg aatctatggt tttttccaaa tccttgtgca tccagatatg agtagtgatg
     1261 ttgtgaaaat caatcattta agtgatgatt actatgtctt ttataatctt cctcaaaaac
     1321 ttaatgtgtt ggatttcggc ggtttaatta atattgtttc cccgattcat ataaaagtaa
     1381 acggtaaaat tcatcgttta cttagcaaaa tttatcgccc cagtgcggaa aaagaacgct
     1441 atgttgttca cgggaaagct ttctttgctg ccggggttaa actaaatccc cagtttatgg
     1501 gaacgggttt taataaggaa gttcgggtta ttggcgactt tggcagtcgc ttgtatatct
     1561 ttaccaagat aaatcataaa tatcgtttaa aaaggactaa gttaatgaac tgatatcagt
     1621 ttaattaact agtccttttt gtttaaggta tagctttgtt ttggttggta taaaaccaga
     1681 tagtaatgat ttgtttcttt gtttgaccaa gttatcaagc ggctattatg cattaaattt
     1741 tgcttagata attgaggata ttgctgttta aaaaggtcta aaacggcttt ggtttggctt
     1801 ttagtaagct cataatccca ccaaagcggc ggtttaagtt tagcattatc caaataatcc
     1861 tgttttaata aaaataaaaa ataatcttgg ttcttagttg tgatggcttt gtaaaaggct
     1921 ttggctaaat ctttaatttg taaacgacat tccggatgta aggaaagata agttccgagc
     1981 gttaaaaaca attggtaggg atttaactga caatctttaa gtaaatagtc caatgttttg
     2041 ggaaaacgat gggaattgta ataacggttt aatccttctt ccacaagctt aatttccgct
     2101 aaatcttttc tggtcaaata tttattttct aaaacatcat aaggcggtaa agaattaaaa
     2161 acataggcgt gcaaagcttt ctcggcaaca attttc
//
LOCUS       DAOSGN010000124         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_49348_length_2193_cov_2.807297, whole genome shotgun sequence.
ACCESSION   DAOSGN010000124 DAOSGN010000000
VERSION     DAOSGN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49348_length_2193_cov_2.807297"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PK087_02350"
     CDS             <1..301
                     /locus_tag="PK087_02350"
                     /inference="COORDINATES: protein motif:HMM:NF018087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HOH18145.1"
                     /translation="CLRELTTFCGRIQAVKNLFGFKYHPPLYINDHLLLFKLKTAHEH
                     YWINYYQIFEIVNNKGFTKLIFKNQSVLILDFPFHLSKKRLEKAMLIADYKNGLH"
     gene            376..1587
                     /locus_tag="PK087_02355"
     CDS             376..1587
                     /locus_tag="PK087_02355"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOH18146.1"
                     /translation="MSKKTIKDVQLTGKTVLIRVDFNVPMKNGQITDQNRIVQALPTI
                     KYVLEQKAKIVLFSHLGRVEKEEDKLTNSLAPVAKRLSELLAKPVLFINETRGPKLEA
                     AVKNMKVGDILMFENTRFEDLNGKAESKNNSELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIANNSDEVVAGFLMEKEIKFIGDAVDHPVRPFVAILGGAKVSDKISVIENLLTKA
                     DKILICGAMAYTFLKAQGLEIGLSKCEDDYVEFAKSLLEKGRRKLILPVDHAVALDFS
                     DQATFEVVDVDKTPADRMGLDIGPKTIALFKKELQGAKTVVWNGPAGVFEFAQFAYGT
                     KSICQLLAELPNATTVIGGGDSAAAAIKFGFENQFTHISTGGGASLEYLEGKELPGVA
                     AINDVCCCGRC"
     gene            1603..>2193
                     /gene="tpiA"
                     /locus_tag="PK087_02360"
     CDS             1603..>2193
                     /gene="tpiA"
                     /locus_tag="PK087_02360"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOH18147.1"
                     /translation="MRKPIIAGNWKMFKVRDEALQFIYSVSDQAPSQELVDSVICAQF
                     TVLRCLAKRQGENIRIGAQNMHYLDEGAYTGEVSASMLKAIGVSYVIIGHSERRAMFN
                     ETDKTVNLKLHKAFQQELTPILCIGESLEQREKNLTNRVLNRQLKADLAGLTGEQVAN
                     LVIAYEPIWAIGTGKTATSEVANEACKHIRKQVAKMY"
BASE COUNT          691 a          389 c          482 g          631 t
ORIGIN      
        1 ttgtttgcgg gagttaacta ctttttgcgg tcgtatccaa gctgttaaaa acttgtttgg
       61 ttttaaatat catccgccgc tttatattaa tgatcattta ctgctgttta aattaaaaac
      121 ggctcatgaa cattattgga ttaattatta tcagattttt gagattgtga ataataaagg
      181 atttacgaaa ctgattttta aaaatcaaag tgttttaatc cttgactttc cctttcatct
      241 aagcaaaaaa agactggaaa aggcgatgtt gattgccgat tataaaaacg gtttacatta
      301 aagttttcat ctatacagaa tcgtgatttc gtgttataat aacacgaatg aaaattaaaa
      361 acaggagaag aaaagatgag taaaaaaacg attaaagatg ttcaattaac tgggaaaacg
      421 gttttaatcc gggttgattt taatgtgccg atgaaaaacg gccagattac cgaccaaaac
      481 cgcattgttc aagccctccc aacaatcaag tatgttttag aacaaaaagc caaaattgtg
      541 ctcttctcac atcttggccg tgttgaaaaa gaagaagaca aattaactaa ttccttagct
      601 ccggtcgcga aacgattaag cgagttatta gctaaacccg ttttattcat taatgaaacg
      661 agaggaccga aattggaagc cgccgttaag aacatgaaag taggcgacat ccttatgttt
      721 gaaaacaccc gttttgaaga tttaaacggg aaagcggaat caaaaaacaa ttcggaatta
      781 ggaaaatatt gggcttcgct cggtgatgtt tttgtgaatg acgcctttgg gaccgctcat
      841 cgtgcccatg cttccaatgt cggcattgcc aataatagcg atgaggttgt ggcgggtttc
      901 ttaatggaaa aggaaattaa atttattggg gatgctgttg accatccggt tcgtcccttt
      961 gtggccattt taggtggtgc taaagttagc gataaaattt ccgttattga aaacttatta
     1021 actaaagccg ataaaatctt aatttgtggg gcgatggctt atactttctt gaaagcacaa
     1081 ggtttggaaa tcggtctatc aaaatgcgaa gatgattatg tggaatttgc gaaatcgttg
     1141 ttggaaaaag gacggagaaa attaatcttg ccggttgatc atgccgtcgc tttagatttt
     1201 tccgaccaag ccacttttga agtcgtcgat gttgataaga ctccggctga ccgcatgggc
     1261 ttagatatcg gaccaaagac aattgctttg tttaagaaag aacttcaagg ggctaaaact
     1321 gttgtttgga acggtcccgc cggcgttttt gaatttgctc aatttgccta cggaacaaag
     1381 agtatctgcc aattgttagc ggaactaccg aatgcaacca ccgtcatcgg tggtggcgac
     1441 agtgctgccg ccgccatcaa atttgggttt gagaatcaat tcacccatat ctctaccggt
     1501 ggcggtgcaa gcttagaata tttagaagga aaagaattac cgggcgtagc agccatcaat
     1561 gatgtttgct gttgtggccg ttgttaatga taaggagata aaatgagaaa accgattatt
     1621 gctggtaatt ggaaaatgtt taaagtccgg gatgaagctt tgcaattcat ctattcggtt
     1681 tctgaccaag cccccagcca agaattagtg gacagtgtca tctgtgccca atttaccgtt
     1741 ttgcgctgct tagcaaaaag acagggcgaa aatattcgca ttggtgctca aaatatgcac
     1801 tatttggatg aaggagctta taccggtgag gtatcagcct cgatgttaaa agcgattggc
     1861 gtcagctatg tgattattgg ccacagcgaa cgccgggcca tgtttaatga aacagataaa
     1921 actgttaacc taaaacttca caaagctttc caacaagaat taacaccgat tttatgtatt
     1981 ggggaaagcc ttgaacaacg cgaaaaaaac ttaactaacc gcgttttaaa taggcaatta
     2041 aaagctgatt tagccggttt aacaggggaa caagtcgcta atttagtcat tgcttatgaa
     2101 ccgatttggg caatcgggac cggcaaaacc gctactagtg aagttgccaa tgaagcttgc
     2161 aaacatattc gtaaacaagt tgccaagatg tat
//
LOCUS       DAOSGN010000125         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_49479_length_2189_cov_2.143861, whole genome shotgun sequence.
ACCESSION   DAOSGN010000125 DAOSGN010000000
VERSION     DAOSGN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49479_length_2189_cov_2.143861"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PK087_02365"
     CDS             <1..1236
                     /locus_tag="PK087_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH18148.1"
                     /translation="SLLMKNILIFLMKIFSKKMPDLNWENPKVQEEMVQMATWWCEQG
                     IDGFRIDAISHLAKAEFVDSDLFPDHEYKPDWSKYSNLPRLHDYLKLLNKRVFSKYNI
                     VTIGEVGGNAPVSEGIAYTNPKNHELNMVFNFDHNWCNNAWGAQSKKELSTDVQQLKA
                     VFHKWQTGLYGQGWNAIYWLNHDQPRLMSQYGDPEHYHQESAKMLETVLYFMGGTPFI
                     YNGEEIGMTNPEFKSIADFRDVSIINQYEINVKRHHQDEQAFLARAALTSRDNARSLM
                     QWSDQADGGFSLVKPWNIIGDYHKINVAKQINDPQSIFTYYRRIIALRKSDQYRDVLV
                     YGTYEGLWMEHPDLFAYMRSYAHQRLLVIANMTKKTVALPSIPYQQVKIILANYEGRQ
                     LSPALLPYEAMLIQIEEED"
     gene            1238..>2189
                     /gene="malQ"
                     /locus_tag="PK087_02370"
     CDS             1238..>2189
                     /gene="malQ"
                     /locus_tag="PK087_02370"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOH18149.1"
                     /translation="MRGSGLLLHISSLPNPYGIGTLGKEAYQFVDFLKKAGQKYWQVL
                     PLGPTSYGDSPYQTFSAFAGNPYFIDLDQLCKEKLLKKSEYQILKTDPSASIDFSFQY
                     QHRFVILKLAFERFLKASNHDQFNQFISDNQMWLADYALFVAIKEHMPEGNWHLWDYE
                     LKIRDPKALKRFQKDNARDIQFWMFLQYKFFEQWHALKNYANEKGIKIIGDMPIYVAY
                     DSSDVWANPKYWQLDQELNPLSVAGVPPDHFAITGQLWGNPLYNYDLMEKDGFKWWIE
                     RIRESLKLFDVVRIDHFRGFEAYYSVPFTDKTAENGHWVKG"
BASE COUNT          664 a          401 c          466 g          658 t
ORIGIN      
        1 agccttttaa tgaagaatat tttgattttt ctgatgaaaa ttttttccaa aaaaatgccc
       61 gacttaaatt gggaaaaccc caaagtccaa gaggaaatgg tgcaaatggc cacttggtgg
      121 tgcgagcaag gcattgacgg ttttcgtatt gatgccattt cccacttagc gaaggctgaa
      181 tttgtggatt cagacttatt ccccgatcat gaatataaac cggattggag caaatattcc
      241 aatcttcctc gccttcatga ttatttaaaa ttacttaaca aacgggtttt ttcgaaatat
      301 aatattgtga cgattggtga agtggggggg aatgcccccg ttagcgaggg gattgcttat
      361 accaatccta agaatcacga actgaatatg gtctttaatt ttgaccacaa ttggtgtaac
      421 aatgcttggg gagcccaaag caagaaagaa ttgtccaccg atgtgcagca attaaaagct
      481 gttttccaca aatggcaaac cggcttatat ggacaaggct ggaatgctat ctattggctc
      541 aatcatgatc aaccgagatt gatgtcccaa tatggtgatc ccgagcatta tcaccaagaa
      601 agtgctaaaa tgctggagac cgtcctttac tttatgggcg gaacaccctt tatttataat
      661 ggcgaagaga ttggcatgac caatcctgag tttaaatcaa ttgctgattt tcgggatgtt
      721 tccattatta accaatatga aatcaatgtt aagcgccatc atcaagacga acaagctttt
      781 ctagccaggg cggccctcac ttcacgggat aatgcccgca gtttgatgca atggagtgat
      841 caagccgatg ggggattttc tttggtaaaa ccatggaaca tcattggtga ttatcataaa
      901 atcaatgtgg cgaagcaaat aaatgatccc caatcgatat ttacttacta tcgaaggatt
      961 attgcgttgc ggaaaagcga tcaatatcgt gatgttttgg tttatgggac ttatgaagga
     1021 ctatggatgg aacatccgga cctttttgct tatatgagga gctatgctca ccaacggctt
     1081 ttggttattg ctaatatgac taaaaaaacg gtggctttac cgtcaatacc ttatcaacaa
     1141 gttaaaatca tattagcaaa ttatgaggga agacagttaa gtcccgccct attgccttat
     1201 gaagccatgc ttatccaaat tgaggaggaa gattaacatg cgaggaagtg gccttttatt
     1261 acatatttct tcattaccca atccctatgg aattggcact ttgggaaaag aagcctatca
     1321 atttgtggac tttttaaaga aagccggaca aaaatattgg caagttttgc ccttaggacc
     1381 aacttcttat ggtgattcac cctatcaaac tttttccgct tttgccggca acccctactt
     1441 tattgacctt gatcaattat gtaaagagaa attactgaaa aaaagcgaat atcaaatttt
     1501 aaaaacagat cccagtgcta gtattgattt ttcgtttcag tatcagcacc gctttgtgat
     1561 tcttaaatta gcttttgaac gctttttaaa agcgagcaat cacgatcaat ttaatcaatt
     1621 tattagcgat aaccagatgt ggttagccga ttatgcttta tttgtggcca ttaaagagca
     1681 tatgccagaa ggaaattggc atctttggga ttatgagtta aaaataagag accccaaggc
     1741 gcttaagcgc tttcaaaagg ataatgcgcg agatatccaa ttttggatgt ttctccaata
     1801 taagttcttt gaacaatggc atgctttaaa gaactatgcc aatgaaaagg gcattaaaat
     1861 cattggtgat atgccgattt atgtcgctta tgattccagt gatgtatggg ctaatcctaa
     1921 gtattggcaa ttggaccaag aactgaatcc gcttagtgtg gccggggttc cccccgatca
     1981 ctttgccatc accggtcaat tgtggggcaa tccgctttat aattatgact tgatggaaaa
     2041 agacggtttt aaatggtgga ttgaaagaat cagggaaagt ttaaaattgt ttgatgtcgt
     2101 tcgaattgac cattttcgtg gttttgaagc ttattattcc gttccgttca cggataaaac
     2161 ggcggaaaac ggtcattggg tgaagggcc
//
LOCUS       DAOSGN010000126         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_49822_length_2178_cov_3.002355, whole genome shotgun sequence.
ACCESSION   DAOSGN010000126 DAOSGN010000000
VERSION     DAOSGN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49822_length_2178_cov_3.002355"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK087_02375"
     CDS             <1..198
                     /locus_tag="PK087_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18150.1"
                     /translation="RERLSRGSGRPYPEVNALIKRFEDMKTQMSAFTKMSENPQARPT
                     MPNVQRKASKGKGKGRGNFRF"
     gene            207..2033
                     /locus_tag="PK087_02380"
     CDS             207..2033
                     /locus_tag="PK087_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18151.1"
                     /translation="MNFKEFEERISNINNQIFAFFMQIEEEENKQINDVFQSLKQNLD
                     SQKNILVDFERQLKEDDDQYQSKIAALEKEILNIASEPETYLHILEEKKAFLSQKKND
                     EQKRILALQRRRRSEFAHRINTLNKELSLLLKEHNNQLEEEEKNYKAKETELMRRMNI
                     DLQKANEKTVKASSDLEKQLLETNDLSGIAEIKKKIVAIRTEGFKEQESIKNKYEFSF
                     IENTLDFKRFYEKISLDNALTQEDFAVKIKALEKQRNDLELAEQLEFALFGFELDKQY
                     NEWEKEQNLHAIEWQIQNFAQQMLIKNQIQDEKHQYDLHHFENNKNYLQKAHEVDTMQ
                     FASYRPLELLISNNIKKQCDCLLEGLADCIEIFQAHLIAIVENTWNLKKSLRNDLSKF
                     LLVTIKNELPLIEPHYSHELTKIEHFCLEYYKLQNKRFKNFLRSVASQAHNLLQEVKA
                     TMAIFKNYYLEEQAYREEMDKKMNSSLLKSYENALLYLAGDYHQEENNEKNYHRQVQD
                     EKEKHRLSMELKKQTAMKLYHDQLKAIEEKSKKATLENQRLVADEENNYQLFIKNSKQ
                     KQSAFKRDFSDHLILHERSLDKQYRLALQRNEKERKAKIKAL"
BASE COUNT          840 a          337 c          387 g          614 t
ORIGIN      
        1 cgggaacgct tgtctcgggg ttcgggtcga ccttatcctg aagtcaatgc tttgattaaa
       61 cgctttgaag atatgaaaac ccaaatgtcg gcgtttacga aaatgagcga aaatccgcaa
      121 gcccgcccca caatgcccaa tgttcaacgc aaagcaagca agggtaaagg caaaggtcga
      181 ggtaattttc gattttgagg tgaaatatga attttaagga atttgaagaa agaatcagca
      241 atatcaataa ccagattttt gcttttttca tgcaaattga ggaagaggag aataagcaaa
      301 ttaatgatgt ttttcaatcc ttaaaacaaa acctcgattc ccaaaaaaac attcttgttg
      361 attttgaaag gcaactaaaa gaggatgatg atcaatatca aagtaaaatt gctgctttgg
      421 aaaaagaaat attaaatatt gctagcgaac ccgaaactta tttacatatc ttggaagaaa
      481 aaaaagcttt tctaagccaa aagaagaatg atgaacaaaa acgtattttg gctttacaaa
      541 ggcggcgtcg cagtgaattt gcccatcgca tcaacactct aaacaaggaa ttgtcgcttt
      601 tattaaagga acataataat caattagaag aagaagaaaa aaactataaa gccaaagaaa
      661 cggaattaat gcgccgaatg aatattgatt tgcaaaaggc gaatgaaaaa acggtcaagg
      721 cgtccagcga tttagaaaaa caacttcttg aaaccaatga tttaagcggg attgcggaaa
      781 tcaaaaagaa aattgtagcc attcggactg aaggctttaa ggaacaggaa agcatcaaaa
      841 acaaatatga attttccttt attgaaaaca ccttggattt taaacgcttt tacgaaaaga
      901 tttccttgga taatgcttta acgcaagaag attttgcggt taagattaaa gcactcgaaa
      961 aacaaaggaa tgatttagaa ttagcggagc agctggagtt tgctttattt ggttttgaat
     1021 tggataaaca atataacgaa tgggaaaaag aacaaaattt acacgccatt gaatggcaaa
     1081 tccaaaattt tgctcaacaa atgcttatta aaaaccaaat tcaagatgaa aaacatcaat
     1141 atgatttgca tcattttgaa aataataaaa actatcttca aaaagctcat gaagtcgata
     1201 ccatgcaatt tgcatcatat cgacccttgg aattattaat ttctaataat atcaaaaaac
     1261 aatgcgattg cttgctagaa ggtcttgcgg actgcattga aatattccaa gcccatttaa
     1321 ttgcgattgt ggaaaatact tggaatttaa aaaagagttt gcgtaatgat ttatcgaagt
     1381 ttttattggt gaccattaaa aacgaattgc cgctcattga acctcattat agccatgaat
     1441 tgacgaaaat tgagcacttt tgtttagaat attataaatt acaaaacaaa cgatttaaaa
     1501 attttttacg gtctgttgct tcccaagccc acaatttact tcaagaagta aaagcaacca
     1561 tggctatttt taaaaattac tatcttgagg aacaagctta ccgagaagaa atggacaaaa
     1621 aaatgaattc gtcgttatta aagagttatg aaaatgcttt gctttattta gcgggtgatt
     1681 atcatcaaga agaaaataac gaaaaaaatt atcatcgcca agtccaagac gaaaaggaaa
     1741 aacatcgttt gagtatggaa ttaaaaaaac aaacggcgat gaaactttat catgaccaac
     1801 taaaagccat tgaagaaaaa agtaaaaagg cgactttgga aaaccaacga ttagttgccg
     1861 atgaggaaaa caattatcaa ttatttatta aaaattccaa acaaaaacaa tcagctttta
     1921 aacgcgattt tagtgatcat ttaattctcc atgagcgttc tttggataaa caatatcgtt
     1981 tggctttaca aaggaacgaa aaagagcgta aagctaaaat caaagcatta taaaaaacgc
     2041 gttttatcgc gttttagatt aattttttta aaacaatttt aattagttca ttaatgggtt
     2101 tatcaatatt ggcttccatg gttgcttgca tggctttgat ttcgttgttg ctataaccca
     2161 tggcttttag agcatccc
//
LOCUS       DAOSGN010000127         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_50113_length_2170_cov_2.355083, whole genome shotgun sequence.
ACCESSION   DAOSGN010000127 DAOSGN010000000
VERSION     DAOSGN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50113_length_2170_cov_2.355083"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2032
                     /locus_tag="PK087_02385"
     CDS             <1..2032
                     /locus_tag="PK087_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18152.1"
                     /translation="TVDIHDYYHLTLAKKLYVEDLVRARYAEKDFTDTEYENIYNNNY
                     KPHYQALIVTYPTQKTLDNALLQLGVKIVDGVWQKATTSVALTEQEIVATFIALYNNQ
                     HAYELSDYPNATLTLVDGEEYDSSSGSIVFDLTKIDELSYTHTELNNFQGELINLFTK
                     MGNYPEAGFYTTSPKIYKNGSRYLLALKIAQDQATLESVKAEIREKLIKAAVTTSLIE
                     TETINLRSAHALKIYDKPLETTYVAKVEAAKLTFKPTKKSSDHLVFATDVQTYSADDL
                     FEKMNRRYGINIAISELDYLRLINSQTFNNIYDLNTKTVFDKTTWDVILQQVKDEKAN
                     FNNDVYAEYGYPKSYGWTKFLQDIYSVNSEEELSQYYLYLEIRDRFTASLGDLSTATE
                     TSALWSFYQNQMQKVVDDYYSVKGVHLLIAHYEGSNLVNPSKWTDYQKAMAEEFYRAI
                     MNYLKTESGTYVEKLQALELAFAKAPVFVATKPQTTAGQDVIDGINYTFKDIEIAKYK
                     SAGLTILYQDLGTFVNGTMVAEFSDAVRTLWKTDPTSKTPTVYGLNPDGQKNWSYLVT
                     EFGYHVYVNLESYPLSGWEVDKYIPTLEQIQLYLEDPSTDGLTVAQKTAITTYFTPIK
                     TELTGTNNVSAQSYRQMMTANISFQMATFTNADFLRQMALKLATYEDQLKYR"
BASE COUNT          712 a          400 c          424 g          634 t
ORIGIN      
        1 tacggttgat attcatgatt attatcattt aactttagca aagaagcttt atgtagaaga
       61 cttagttcgc gctcgttatg cggaaaagga ttttaccgac accgaatatg aaaatatcta
      121 taacaataat tataaaccgc attaccaagc cttgattgtt acttatccga cgcaaaagac
      181 tttggacaat gctttgttgc agttgggcgt gaagattgtt gacggggttt ggcaaaaagc
      241 taccacctcg gtggctttaa ctgaacaaga aattgtggcg accttcattg ctttgtataa
      301 caaccaacac gcttatgaac ttagtgatta tcccaatgcc actttaactt tggtcgatgg
      361 cgaagaatat gattctagca gtggctcgat tgtgtttgat ttaactaaaa ttgatgaatt
      421 aagctatacc catacggaac ttaataattt ccaaggggaa ttaatcaatt tgtttacgaa
      481 aatgggaaat tatcctgaag ccggttttta tacaacaagc ccgaagattt ataaaaacgg
      541 ttctcgttat ttgttagcac taaagattgc tcaagaccag gcaaccttgg aaagcgtcaa
      601 agcagaaatc cgggaaaagt taatcaaagc ggccgtgacg acctctttga tagaaacgga
      661 aaccatcaat ttacgctccg ctcatgcttt aaagatttat gataagccat tggaaaccac
      721 ttatgttgct aaagtggaag cggccaaatt aacatttaag ccaaccaaga aaagcagtga
      781 tcacttggtt tttgcgaccg atgtccaaac ctatagtgcg gatgatttat ttgaaaaaat
      841 gaaccgtcgt tacggcatta acattgccat tagcgaactg gattatttac gcttaattaa
      901 cagtcaaact ttcaataata tttatgatct taataccaaa actgtttttg ataaaaccac
      961 ttgggatgtc atcttacaac aggtgaaaga tgaaaaagcc aacttcaaca acgatgttta
     1021 tgcggaatac ggctatccta aatcctatgg gtggaccaaa ttcttgcaag atatttatag
     1081 tgttaattcg gaagaggaat tatcccaata ctatttatat ttagaaatca gagatcgctt
     1141 taccgcctct ttaggtgatt taagcacggc tacggaaact tcagcgcttt ggtcgttcta
     1201 ccaaaaccaa atgcaaaaag ttgtggatga ttattacagc gttaaaggcg tgcatctgtt
     1261 aattgcgcac tatgaaggca gtaatttagt taatccgagc aagtggacgg attatcaaaa
     1321 agccatggct gaagagtttt accgcgcgat tatgaattat ttaaagacag aaagcggaac
     1381 ttatgtggaa aaacttcagg ctttagaact ggcgtttgcg aaagctcctg tttttgtcgc
     1441 cactaaacca caaacaacag ccggccaaga tgtgattgat ggcattaact atacttttaa
     1501 ggacattgaa attgccaaat acaaatcggc gggacttacg attctgtatc aagatttagg
     1561 aacctttgtt aatggcacga tggttgcgga attctccgat gcggttcgga cattatggaa
     1621 aactgatcca acttcgaaaa caccaactgt ttatgggctt aacccggatg gtcaaaagaa
     1681 ttggtcctac cttgttaccg aatttggtta tcatgtttat gtcaatttag aaagttatcc
     1741 tttaagcggc tgggaagttg ataagtatat tccaacgctt gagcaaatcc aactatattt
     1801 ggaagatcca tcaacagacg gattaacagt tgcacaaaaa accgccatca caacttattt
     1861 cacgccgatt aaaacggaat tgacgggcac caataatgtt tctgctcaat cataccgaca
     1921 gatgatgact gccaacatta gtttccaaat ggccactttc acaaacgcgg acttcttaag
     1981 acaaatggcc ttgaaattag ctacttacga agatcaatta aaatatcgat aacagtaaaa
     2041 cttcggttca gccgaagttt ttatcttttt gttggtattg ataatatgcg ataaatatcg
     2101 tataattaat aaatatcaga ggaaaaacaa tgagtgtttt taaactacat agtgattatg
     2161 ttccgaccgg
//
LOCUS       DAOSGN010000128         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_50402_length_2161_cov_2.477208, whole genome shotgun sequence.
ACCESSION   DAOSGN010000128 DAOSGN010000000
VERSION     DAOSGN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50402_length_2161_cov_2.477208"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PK087_02390"
     CDS             complement(<1..361)
                     /locus_tag="PK087_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100684.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HOH18153.1"
                     /translation="MELRNKKWTEAEFFKVRQQVLASWPTGSSELLNLDMAIANLKKI
                     PAHKNFALKMAEAKKQGRTLVQPRAGVADIDRHIELMQFLEAAGADFLPSTIDSYTRQ
                     NRYAEAERGIEESRRRNR"
     gene            complement(366..1745)
                     /gene="glmL"
                     /locus_tag="PK087_02395"
     CDS             complement(366..1745)
                     /gene="glmL"
                     /locus_tag="PK087_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HOH18154.1"
                     /translation="MNACLLIDFGSTYTKLTAVDLDLGILLGTSSHFTTVSSDIRLGY
                     HRALSLLKEKIGVIPFSKIIACSSAAGGLKMAAVGLVEELTVEAAKRACYGAGGKVDL
                     VFSHFLTKTDIDLMKTKKIDIVLLAGGTDGGNSEVVIHNAKMLASANLKIPIIFAGNK
                     AAQDDIRDIFTEKQVNGFICENIMPKLNVLNIDKAKEVVRKIFLENIIVAKGIKQIES
                     EIDAVMLPTPYAVLKAAELLSKGYLHEEGLGDLVISDVGGATTDLYSLGYGYPKRADV
                     VLKGLSEPFAKRTVEGDLGMRYSALGIDATLSEQERALYLEQGIDMRKEAQFRHDHVD
                     MIALSPYDCEIDRLFGGICHDKAMSRHVGKMEQAYTPMGLLYYQTGKDLTQVKYIIGT
                     GGVVIHDEKPQEMLLKLCADNQKPLELRPVKPQFLIDRQYVLSAMGLLSIDHPEIALK
                     IMKGSLEKI"
     gene            complement(1742..2149)
                     /gene="glmS"
                     /locus_tag="PK087_02400"
     CDS             complement(1742..2149)
                     /gene="glmS"
                     /locus_tag="PK087_02400"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HOH18155.1"
                     /translation="MKTIVIGVIGADVHAVGNRIIEYVLTNEGYNVVNIGVLSTQEDF
                     INAAIETNAQMIMVSSLYGHGELDCVGLREKCVEAGIGEILLYVGGNIVVGKQDFETV
                     EKRFKDMGFNRVFPPGVTIDQVLPTIKADLETR"
BASE COUNT          658 a          486 c          361 g          656 t
ORIGIN      
        1 tccgattgcg acgacgactt tcctcaattc ctctttctgc ttcagcatag cgattttgtc
       61 tagtataact gtcgattgta gaaggaagaa aatcagctcc cgctgcttct aaaaactgca
      121 tcaattcaat atggcgatca atatcggcga cacccgctcg cggttgaaca agggttcgac
      181 cttgtttttt cgcctcagcc attttaagag caaagttttt atgagccgga attttcttta
      241 aattagcgat ggccatgtcg agatttagta attcactgct tcctgttggc caagaagcaa
      301 gcacttgttg acgaacttta aaaaattcag cttcggtcca ttttttattg cgcaattcca
      361 tttatctaaa ttttttctag gctacctttc attattttaa gagctatttc aggatggtca
      421 atacttaaca atcccattgc tgaaagaaca tattgacgat caattaaaaa ctggggctta
      481 acaggacgga gttctaatgg tttttgatta tccgcacaca attttaagag catttcttgg
      541 ggcttttcat catgaatgac aaccccaccc gtaccaataa tatattttac ttgggttaaa
      601 tcttttccag tttggtaata aagcagtccc atcggggtat aggcttgctc cattttacca
      661 acatgacgac tcatggcttt atcatggcat attcccccaa aaagacggtc gatttcacaa
      721 tcataagggc taagggcgat catatcaaca tgatcatgac ggaattgggc ttcttttctc
      781 atatcaatcc cttgctctaa gtataaagct cgttcttgtt cacttagggt cgcatcaatt
      841 cccaaagcgg aataacgcat tcccaaatca ccttccactg ttcgtttcgc aaacggttca
      901 gaaagacctt ttaaaaccac atcagcgcgc ttgggataac cataacccaa tgaataaaga
      961 tcagttgtcg ccccaccgac atcggaaatc actaaatccc ccaagccttc ttcatgaaga
     1021 tatcctttgg ataataattc tgcggccttt aaaacggcat agggagttgg aagcataacg
     1081 gcatcaattt cggattcaat ttgtttgatg cctttggcaa caataatatt ttccaaaaaa
     1141 atctttctaa ctacttcttt cgccttatca atattgagaa cattaagttt gggcataatg
     1201 ttctcacaaa taaaaccatt aacttgtttt tcggtaaaga tatcgcggat gtcatcttga
     1261 gcggctttgt taccagcaaa aataatagga atttttaaat tagctgaagc caacattttc
     1321 gcattatgaa taacaacttc gctattgcca ccatcagtgc ctccggctaa taagacgata
     1381 tcaatttttt tggttttcat taaatcaata tccgtcttgg ttaggaaatg cgaaaaaact
     1441 aaatcaactt tcccaccagc accataacaa gcccgtttgg cagcttcaac agttaattct
     1501 tccactaagc caacagcggc cattttcaat ccaccagctg cggaagaaca agcaatgatt
     1561 ttagaaaagg ggataacacc aattttttcc tttaataacg acaaagcacg atgataacca
     1621 agccgaatat ctgatgaaac agttgtaaaa tgggaggatg tccccagcaa aattcctaaa
     1681 tcaaggtcaa cagctgttaa ttttgtataa gtcgagccaa agtcaattaa aagacaagcg
     1741 ttcatcttgt ttccaaatcg gcctttatcg tcggcaaaac ttggtcaatg gtgactcctg
     1801 gggggaaaac acggttaaaa cccatatctt taaaacgttt ttccaccgtt tcaaaatctt
     1861 gtttaccgac aacaatatta ccacccacat ataaaaggat ttcaccaatg ccagcttcta
     1921 cacatttttc acgcaggcca acacaatcaa gttcaccatg gccatataat gatgaaacca
     1981 ttatcatttg tgcatttgtt tcgatagctg cattgataaa atcttcttgc gttgataaaa
     2041 cccctatgtt aacgacatta tacccttcat ttgttaatac atactcaata atccggttac
     2101 caacagcatg aacatcggca ccgattaccc caatgacaat cgttttcatg gttttctcct
     2161 t
//
LOCUS       DAOSGN010000129         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_50404_length_2161_cov_2.413105, whole genome shotgun sequence.
ACCESSION   DAOSGN010000129 DAOSGN010000000
VERSION     DAOSGN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50404_length_2161_cov_2.413105"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1958
                     /locus_tag="PK087_02405"
     CDS             222..1958
                     /locus_tag="PK087_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOH18156.1"
                     /translation="MTATSKLNPELSTTKQITIVFRNYLYYQTKILMIDQVNYQMELL
                     DCPTTKFSATTKVKQKLGDKIINLSLSDLMLGMENVYAQIDVEANLISALLIDGEIGF
                     SNIRVGIRKAINDIALDATLYHDVVEFSVLENMVLKTFDGAQSLAISEGSNLTFTYLN
                     GQIEVKRGNDIIMKTAKRLLLQSLGNNENIQFLSITRANGKPYYHGNMEVAIKNNRLL
                     VVNDLNLEKYLYKVLPSEMPSSFGLEALKAQAIAARTYAYMDVFNKATQAYGYTVDDS
                     VKSQVYNNQNTNEISKRAVNETAGQIMTYNGTPVSAYYYSTSSGLTASGHEVWISKQV
                     GSEIPYLIGQNLAQDDLGKTISFDYRDEASMLAFFKTIKMKTPESALSKYHRWKVSFT
                     KTQLTTTLNKNLKLTYSATPQLVLTKKGDSWLSQTIPSSIGDVTNIYVGERGSSGVVI
                     SLIVETTTGIYKIINQYNIRFTVRPKDAGSTVYRYCAKGTSTTYDPNPSANDSTLLSG
                     FFAIEAEGESFVFYGGGYGHGVGMSQYGAYGLANAGKNYAEILSTYYSNIAFKDISYS
                     YTPIDDYQEYFH"
BASE COUNT          660 a          387 c          443 g          671 t
ORIGIN      
        1 gcgtttataa agaagtgctt gttagcgttg ttcatggtcc tcttgcgacc ttagttattg
       61 aggggggaga agaagtttat gttgggtcaa cgctcgcctt aaaggctttt gatgatcatg
      121 aagatgttag tgaatatatt gattagacaa ttgatgatga gcaaaaagcc accatcaata
      181 cccgaggaat tatttccggc ttagttgccg ggaccgtaat cgtgacggca acttccaaat
      241 taaatccgga attatcgact accaaacaaa tcaccatcgt ttttcgcaac tatttatatt
      301 atcaaaccaa aatcctcatg attgatcaag ttaattacca gatggaatta ctcgattgtc
      361 caacaaccaa atttagtgcg acgaccaaag ttaagcaaaa acttggggat aagataatta
      421 atttgtcctt atccgattta atgctcggca tggaaaatgt ttatgctcag attgatgttg
      481 aagccaatct tatttcggcc ctcttaatcg atggtgaaat tggcttttcc aatattcgtg
      541 ttggcattag gaaggcgata aatgatattg ctcttgatgc gactttatat catgatgtcg
      601 tagagttttc ggttttggaa aatatggttt tgaaaacatt tgatggtgcg caatcgcttg
      661 ccatcagtga aggcagcaat ttaactttca cttacctaaa tggtcaaatt gaagtcaaac
      721 gcggtaatga catcatcatg aagacagcta aacgcttgct tttacaatcc ttaggaaata
      781 atgaaaatat tcagtttttg agtattacgc gggccaatgg caaaccttat tatcacggca
      841 atatggaagt ggcgattaaa aacaatcgtt tattggttgt taacgatctt aacttggaaa
      901 aatatttata taaagtgctg ccgagtgaaa tgccttccag ctttggtttg gaagctttaa
      961 aagctcaagc cattgctgct cgaacctatg cctatatgga tgtctttaat aaagcgacgc
     1021 aagcatatgg atatacagtt gatgattccg tcaaatccca agtttataat aatcaaaata
     1081 ctaatgagat atcaaaaagg gccgtcaatg aaacggccgg acaaatcatg acttataacg
     1141 gaacaccggt ttcggcttat tattattcaa catcttcggg cttgactgcc agcggtcatg
     1201 aggtgtggat ttctaagcaa gttgggagtg aaattcctta cttaattggt cagaatttgg
     1261 cccaagatga tttaggaaag accatcagtt ttgattatcg ggatgaagca agcatgttgg
     1321 cttttttcaa aaccatcaaa atgaagacac cggaaagtgc actttccaag tatcatcgtt
     1381 ggaaagtcag ctttacaaaa acgcaattaa ccacgacgct taataaaaat ctcaaattaa
     1441 cttacagtgc cactccgcaa ctggtgttaa ctaaaaaggg cgatagttgg ctttcacaaa
     1501 ccattccttc atcaattggt gatgttacga atatctatgt tggtgagcgc ggaagttccg
     1561 gggttgtcat cagtttgatt gtggaaacaa cgacgggaat ttataaaatc atcaatcaat
     1621 acaatatccg ctttaccgtt cgtcccaagg atgccggttc cacggtctat cgttattgtg
     1681 caaagggaac aagtacaact tacgacccta atccatccgc caatgactcc actttattat
     1741 ccggtttctt tgcgattgag gcagagggcg aatcctttgt tttttatggt ggtggatatg
     1801 gacatggcgt tggcatgagt caatatggcg cttatggctt ggctaatgca ggcaaaaatt
     1861 atgcggaaat attgtcgact tattatagca acattgcttt taaagacatt agttattctt
     1921 atacgccgat agatgattat caagaatact tccattaaaa aggactccag ttttaaactg
     1981 gaatcctttt ttggtaagtt aagaattttg tgatgattta cagaattctt gatagaccat
     2041 ctttttaaat agcttgttta gctttgattt ccgcaaccat tttttcgtac atggcttcat
     2101 caatgataaa tttcttttta agaatgatgt aagcggtcgt aattaaaagc gtgggaacga
     2161 c
//
LOCUS       DAOSGN010000130         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_50534_length_2157_cov_2.442436, whole genome shotgun sequence.
ACCESSION   DAOSGN010000130 DAOSGN010000000
VERSION     DAOSGN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50534_length_2157_cov_2.442436"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2157
                     /locus_tag="PK087_02410"
     CDS             <1..>2157
                     /locus_tag="PK087_02410"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOH18157.1"
                     /translation="IAVSDDGYWLINGVKTNYKASAETAAPVLLNVTFDPVGGTLPEG
                     TSAVLKNVEKGSTIALPTPTKDGFIFDGWWTGNTVNDGKFANSTTVTKDMTLYARWLM
                     DYVPIKTFFNILDTTNFTAKNKIVANIVAGNVVTKIEHTHNFKVREEGGFIYTYEHIL
                     GQTYQDNVLIPNKGYFNEEFAAYKKEFVAFMFRDEANNPWDFIVQMEHYQPTFPMDFI
                     NPEKFEKREGVNIYDYPLSQKELKIIIQNMEMGDDLTVKSALCHLDLDAYKLVVTLET
                     EELRADDVKEITNVVAEFILSDPGQTIFEIPIADAKLSVLALVDEEVANREEINFAKP
                     ESITAFNAYVDTVKTKINSADSLITLIGLVNTLIPNIATFQFEVDAFAQDKFNRIAEM
                     TNYFEGKVLTATADSIVAMTAIYNTDVANMKSTINITQMENVFDAYFGRINDAFVFDE
                     LKIVLNREKTYHLNWLSVSFGQMLNPYLSSTKDQERLNDTIHQYSTAIESATTVEEIS
                     TLYNQAIEALKTLSLSWQDRVTELPQRLIQNLDYLIDFVRPFVGENEDFEAQVLALAD
                     SINAQNDPVKMVEAYINSSKQLNIIVKQIAIDRLTAKKEQLAPIITDNDYNHLILIYN
                     EALEIINDAPNVVIIMNIVQHFEAEAEDFQIDPVKESIIFRVDLYTRLLASRVRVATA
                     DSIVAMQAIFDEHIPLIKASTTIEQLGERYNTAYRA"
BASE COUNT          696 a          427 c          405 g          629 t
ORIGIN      
        1 caattgcggt ttccgatgat ggttattggc ttattaacgg tgttaaaacc aactataaag
       61 ccagcgccga aaccgcagcg ccggtccttc tcaatgttac ttttgaccct gtaggcggaa
      121 cccttccgga aggaacatcg gcagtcctta aaaatgtgga aaaaggctcg acaattgctt
      181 taccaacccc aaccaaagat ggctttatct ttgatggctg gtggacggga aataccgtta
      241 acgatggcaa attcgccaac tccacaactg ttactaagga tatgacgctt tatgcaagat
      301 ggcttatgga ctatgtgcca attaaaacct tcttcaatat tcttgatact accaacttca
      361 cggccaaaaa caaaatcgtt gccaacattg ttgctggtaa tgtggtcact aaaattgaac
      421 acacccacaa cttcaaagtt cgtgaagaag gcggttttat ttacacttat gaacacatcc
      481 ttggtcaaac ttaccaagat aatgttttga ttccaaataa aggttatttc aatgaagaat
      541 ttgcggccta caaaaaggaa tttgtcgctt ttatgttccg cgatgaagcc aacaaccctt
      601 gggatttcat tgttcagatg gagcattatc aaccgacatt cccaatggat tttatcaacc
      661 cagagaagtt tgaaaaacgc gaaggcgtta atatttacga ttatccgtta tcacaaaaag
      721 aattaaaaat aattattcaa aatatggaaa tgggtgacga tttgactgta aaatccgctc
      781 tttgccattt agatcttgat gcctataagt tagttgttac tctagagacg gaagaactaa
      841 gggccgatga tgtgaaggaa atcacaaatg ttgtcgctga attcattctt tccgatccgg
      901 gccaaacaat ctttgaaatt ccaatagctg atgcgaaact ttccgttcta gcattagttg
      961 atgaagaagt ggccaatcgt gaagaaatca actttgcgaa gccggaatca atcaccgcct
     1021 ttaatgccta tgttgatacc gttaaaacta aaattaattc ggctgactca ttaataacgt
     1081 taatcggttt agtgaataca ttgattccaa acatcgctac cttccagttt gaagttgatg
     1141 cttttgctca agataaattc aaccgcattg ccgagatgac taactatttt gaagggaaag
     1201 tgctcacagc aacggcggac agcattgtgg cgatgactgc tatttacaac accgatgttg
     1261 ccaacatgaa atccactatt aatatcacac aaatggaaaa tgtttttgat gcttattttg
     1321 gtcgcattaa tgatgctttc gtttttgatg aactcaagat tgtcttaaat cgagaaaaga
     1381 cttatcacct taattggttg tctgtgtcct ttgggcaaat gttgaatcct tacttaagct
     1441 caacaaagga tcaagaacgc ctaaacgata ctatccatca atactcaaca gcaattgaat
     1501 cagcaacaac agttgaagaa ataagcactc tttataacca agcgattgaa gctcttaaaa
     1561 ctctatcttt gtcttggcaa gatcgagtaa cggaattgcc acaacgatta attcaaaatt
     1621 tagattattt aatcgatttt gttagaccgt ttgttggcga gaatgaagat tttgaagctc
     1681 aagtattggc attagccgat tcaattaatg ctcaaaatga tccggttaag atggttgagg
     1741 cctatattaa tagttctaag caacttaaca taatcgttaa acaaatagca attgaccgtc
     1801 taacagccaa aaaagagcaa cttgcaccaa ttattactga taatgattat aatcatttaa
     1861 tcctcatata caacgaagct cttgaaataa tcaatgatgc tccaaatgtt gtgataataa
     1921 tgaacattgt ccagcacttt gaagctgaag ccgaagattt tcaaattgat cccgttaaag
     1981 aaagcatcat ttttagagtc gacctttata cgagattact tgcaagtcgg gtgcgcgtgg
     2041 cgactgctga cagcatcgtt gcgatgcaag cgatttttga cgaacacatt cctcttatta
     2101 aagccagcac aacaattgaa caattgggtg agcgttacaa caccgcttac cgtgctt
//
LOCUS       DAOSGN010000131         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_50652_length_2153_cov_3.506196, whole genome shotgun sequence.
ACCESSION   DAOSGN010000131 DAOSGN010000000
VERSION     DAOSGN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50652_length_2153_cov_3.506196"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /gene="asnS"
                     /locus_tag="PK087_02415"
     CDS             complement(<1..1007)
                     /gene="asnS"
                     /locus_tag="PK087_02415"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOH18158.1"
                     /translation="MKLSMRQLSQSFTQLANQFVTVQGWIRTNRSQKEFGFISFNDGS
                     TLATLQIVYDRSLPNFDEVAKWRVGCSLTITGKVVLTPQNKQAYELKAETMILEGDSP
                     EDFPIQPKRHSKEFLREQAHLRPRTNLFNAVFRVRSLLAFAIHEFFRSRDFLYVHAPI
                     ITASDAEGAGEMFHVTTLDLNKLPRLADGSIDYTKDFFGKKTNLTVSGQLEAETFAQA
                     FKNVYTFGPTFRAENSNTPRHASEFWMIEPEMAFADLEDDMKLVEDMIKYVINYILKH
                     AKEELAFFDQFVAPGKLMQLQSLVDSHIFRCEYAEAIKIFEIQNHLFENKIKYGDDLA
                     T"
     misc_binding    complement(1045..1249)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1335..1643)
                     /locus_tag="PK087_02420"
     CDS             complement(1335..1643)
                     /locus_tag="PK087_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH18159.1"
                     /translation="MPKGLDTPAGEAGKLMSGGEKQRFSLLLSLLSNKKILLLDEVTA
                     NLDSNTERIVRDNLKKLIVNEGYTIISVSHRNEFLKYASIIYEIKNGYLISDNRIINN
                     "
BASE COUNT          703 a          387 c          342 g          721 t
ORIGIN      
        1 tccgtagcta aatcatcacc atatttaatt ttgttttcaa acaagtggtt ttgtatttca
       61 aaaattttaa tcgcttcggc atattcacaa cggaaaatat gggagtcgac caatgattgc
      121 agttgcatca gtttgcccgg agcgacgaat tgatcaaaaa aggccaattc ttctttagca
      181 tgttttaaaa tataattgat aacatattta atcatgtctt ccacaagctt catatcatct
      241 tccaaatccg caaaagccat ttccggttca atcatccaaa actcggaagc atggcgggga
      301 gtgttggagt tttcagcccg gaaggtcggt ccgaaggtat aaacattctt aaaagcctgg
      361 gcgaaagttt ccgcttccaa ttgtccggaa accgttaaat tggttttctt tccaaaaaaa
      421 tctttcgtat aatcaatcga cccgtccgcc aaacgcggca gtttgtttaa atcaagggtg
      481 gtcacatgga acatttctcc ggcgccttcc gcatcgctgg cggtaataat cggagcatgg
      541 acatataaaa aatctcgaga acggaaaaac tcatgaatgg caaaagccag taaactacga
      601 actctaaaaa cggcattgaa taaattggtt cgtggtcgta aatgagcttg ttcacgcaaa
      661 aactcttttg aatgacgctt cggttgaatc ggaaaatctt cgggtgaatc gccttccaaa
      721 atcatggttt ccgcttttag ttcataggct tgtttatttt gcggggtcaa aacaacttta
      781 ccggtaattg ttaatgaaca accgacacgc cacttagcga cttcatcaaa gtttggtaag
      841 gaacgatcat aaacgatttg taaagttgca agggttgagc cgtcattaaa actgataaac
      901 ccgaattctt tttgactgcg gttggttcta atccaacctt ggacggtaac aaattgatta
      961 gccaattggg taaatgattg ggataattga cgcatactta atttcataaa atcctcctta
     1021 aaaataaaaa aatccttaag aacacttaag gacgaaaaaa ttccgcggta ccaccttgat
     1081 tcagcaagtt gctgcactta aaacgacgga taacgcagtc agcggttagg tctacttaat
     1141 tcttctaaca actccgagtg ttctttcgcc agctgttgtt tacgggtttc cactatctcc
     1201 gtatcactgt taaaaacgaa ctgggtactt ttctcatcaa cgtttctaaa ttgttgatag
     1261 tactataaca tggggaagaa tatttgtcaa taaaaggctt tttataatct acaaacttat
     1321 ttataacaaa taagctaatt atttataatt ctattatcag aaataagata tccgtttttt
     1381 atttcgtata tgatagatgc atatttaaga aattcatttc tatgacttac cgagataatc
     1441 gtatagcctt catttacaat taattttttt aaattatcgc gaactattct ttctgtattg
     1501 ctatctaaat tagcagttac ttcatctaat aatagtattt tcttattaga taacaaagat
     1561 agtaacaaag aaaatctttg cttttctcct ccagacatta attttccagc ttctccagcg
     1621 ggagtatcta agcctttggg caaactatta atataatcct ctaaacaagc ggtttttaaa
     1681 tttcatcttc acttgctttt tcatttatta ataatatatt ttcttttaat gttccaggaa
     1741 acataaagat atcttgagag acttttgtaa tattgtctct aatggagaaa caagatacat
     1801 cattaatgtc ttgattatca ataaatattg aaccattagg aacttcatac attttcaaca
     1861 ataagtttaa tatagttgat ttaccactgc cgctttcacc tataatacca atccatgaac
     1921 ctttggggat ttctaatgta atacctttta atatgttttt tccttcatca aaagaaaaat
     1981 tacaattgtc aattttgata ctatcattaa atataaaact gtttttattg aaatcttttt
     2041 cttcaccatt taattcaaaa aatgaaaata attttcatat tcagcatttt tcttttttaa
     2101 tgaatattgt aaaatgaaaa tagaaaaaag atttaaagaa caaagggggt tag
//
LOCUS       DAOSGN010000132         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_50656_length_2153_cov_3.034795, whole genome shotgun sequence.
ACCESSION   DAOSGN010000132 DAOSGN010000000
VERSION     DAOSGN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50656_length_2153_cov_3.034795"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1433
                     /locus_tag="PK087_02425"
     CDS             102..1433
                     /locus_tag="PK087_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOH18160.1"
                     /translation="MNKKKISPYVIIILGFIGLILIGSLLLMLPFATYNHVSLSYIDA
                     LFTATSAVCVTGLSAVPTLAGTYTLFGKIVIAILMEIGGLGFVTIVIFIFTLLGLKIG
                     IQDRFLIKESLNQNSLKGIVKLVRFTVFVTLIVQTLGAIINFLVFNRYFSFGKALGIS
                     VFHAISSFNNAGFDLFTEGTSLIPYQNPLLLINTAVLIVIGGIGFIVINDIITNKSWK
                     KLSVHSKIVLLTSLCLIVFGALILKLLEWNNITWMQAFFTSVAARTAGFAVVDFNTIT
                     SSALIVVMALMYIGASPASTGGGVKTTTFYTVIKYISSFARGRLPLTFKRRIATGSVI
                     KAFILVVFSLTFIFLAIFALTLTEQKHLLATATTDNLERIVFEVFSAFGTVGNSMGIT
                     AHLTWGSKLIICLTMFFGRLGPITIISAWNRNWNIDANVGIKYLEEKMIIG"
     gene            1455..2120
                     /locus_tag="PK087_02430"
     CDS             1455..2120
                     /locus_tag="PK087_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOH18161.1"
                     /translation="MKKSFAVLGLGRFGLKLIEELSMHNVDIIAIDVMEENVVKASEF
                     VSSAFVCDSTNEAALKELGVHNVDHAVIAFGSNLQNTILTTIILKEMGIPKITVRVDD
                     EFYVSVMKKLGATDIVSPQKIAGVRLANKIVADTFVDYFNINTEFSVAEISIESDITP
                     LNIQELNPRNRFDVNLLLIKRDQLSLLPKASDSIMPNDHIYVFGATAKIAKFNHYVNS
                     QKG"
BASE COUNT          590 a          392 c          432 g          739 t
ORIGIN      
        1 ttttttgtag aaagaaaaaa agtctaaaaa aaccattcat tgcataaagt aaataataag
       61 cgtataatac caatatagaa aaatctttca tggtgatatg aatgaataag aaaaaaatct
      121 cgccttatgt tatcattatc ttggggttta tcggccttat tttaatcggc tcgcttttgt
      181 tgatgcttcc tttcgcaact tacaatcatg tttccttatc ctatattgat gctttattta
      241 ccgctaccag tgcggtttgt gtgacgggtt tatccgccgt ccccacttta gccgggacct
      301 ataccttgtt tgggaaaatc gtcattgcca tcttaatgga aattggcggt ttgggctttg
      361 tgacgattgt tatctttatt ttcacgctgt taggattaaa gattgggatt caggatcgtt
      421 tcttaataaa ggaatcttta aatcaaaact ccttaaaagg gatcgttaaa ctggtgcgct
      481 ttactgtttt tgttacttta attgtgcaaa cattgggtgc cattattaac tttttagtat
      541 ttaataggta tttttccttt gggaaggctt taggaatcag tgtttttcat gccatatcct
      601 cttttaataa tgccggcttt gatttgttta ctgaaggaac ttccctcata ccttatcaaa
      661 atcccctcct tttaattaac acggctgttt taatcgtgat tgggggaatt ggtttcattg
      721 tcattaatga cattattacc aataaatcct ggaaaaaatt atctgttcat agcaagattg
      781 tgttgttaac atcgctgtgc ttaattgttt ttggggcact tattttaaaa ctgttggaat
      841 ggaataatat cacttggatg caagctttct ttaccagtgt tgcagccaga acggccggct
      901 tcgctgtcgt agactttaac accatcacca gcagtgcttt aattgttgta atggctctaa
      961 tgtatattgg tgcttcaccc gcttccaccg gtggtggcgt taaaacgacc accttttata
     1021 ccgttattaa atatatcagt tcctttgctc gtggtcgctt accgcttact ttcaaacgac
     1081 ggattgctac tggcagtgtc attaaagctt ttattttagt ggttttttcc ttaacattta
     1141 tctttttggc tatttttgct ttaacattga ccgaacaaaa gcatttatta gcgacagcta
     1201 cgaccgacaa tttagaacga attgttttcg aagtcttttc cgcctttggg acggtaggaa
     1261 actcgatggg gattacagct catttaactt ggggcagtaa attaatcatc tgtttaacga
     1321 tgttttttgg gcgtttaggg ccgattacga ttatcagtgc ttggaaccgg aattggaata
     1381 ttgatgccaa tgtcggcatt aagtaccttg aagaaaaaat gattatcgga tagaatttac
     1441 attaggaggt tcatatgaaa aaatcctttg ccgttttagg cttgggccgt tttggcctga
     1501 aattaattga agaattaagt atgcacaatg ttgatatcat tgccattgat gttatggagg
     1561 aaaatgttgt taaggcgagc gaatttgtta gtagcgcttt tgtttgtgac tctacaaacg
     1621 aagccgcttt aaaagaatta ggggtacaca atgtcgacca tgccgttatt gcctttggtt
     1681 ccaacctcca aaacaccatt ttaacgacaa ttattttaaa ggaaatgggg attcctaaaa
     1741 taacggtgcg ggttgatgat gaattctatg tgtccgtgat gaaaaaactg ggggccacgg
     1801 atatcgtctc cccccaaaaa attgccggtg tgcgcttggc caataaaatt gttgccgata
     1861 cctttgttga ttattttaat atcaatactg aattttcggt tgcggaaata tcgattgaaa
     1921 gcgacatcac ccccctcaat attcaagagt taaatccgcg caaccgcttt gatgtcaact
     1981 tgttattgat taaacgggac caactgtcct tgctgcctaa agccagtgac agcattatgc
     2041 ccaatgatca tatttatgtt tttggggcca cggcgaagat tgcgaaattc aatcactatg
     2101 tgaattcgca aaaggggtaa tatcgatgaa gattaccacc ccccttaaag ctg
//
LOCUS       DAOSGN010000133         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_51210_length_2139_cov_3.041267, whole genome shotgun sequence.
ACCESSION   DAOSGN010000133 DAOSGN010000000
VERSION     DAOSGN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51210_length_2139_cov_3.041267"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PK087_02435"
     CDS             <1..963
                     /locus_tag="PK087_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH18162.1"
                     /translation="GYLPNLLANKISYRKLQALWGFVFVLPLIIGFIYFFLLPFLVTI
                     VYSFSRVENLGLNSSTGEHIGIVSEWIGFDNFTYILKEHSTFRQTLTSSFTTAFFNVP
                     LILIFSLIIAVVLNSKFKGRSFVRAIFFMPVIFNSQAVDAAMQTGAQLAAAMEGATAD
                     IFAQMFNFQDFLIDANIPVNAVNFLGTTADQIYDIISYSGVQILIFLAGIQSVPKHLY
                     EAAKIEGATQYEIFWKITFPMVSPLMLTAGVYTVVDSLLRSPLINMINVFATAGVRKL
                     VNGLGDVSYYGIHASMSWLFCISSIIIIAIWLFLLSKLVFYYDE"
     gene            953..1999
                     /locus_tag="PK087_02440"
     CDS             953..1999
                     /locus_tag="PK087_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH18163.1"
                     /translation="MMNKDNKTLKLGTFKQKMQKMKADFKDPTKVKVRSRRAFMVTGS
                     VLRTFILIGLIFVILLPLFQKFSFALRHPMDITNLQVIWIPETISNINFQIAYQLLDF
                     GHTIWNTLILSTFSMIIQIMATAVAGYAFARLRFKGSNIIFYIVLFTLVVPNETLHIA
                     RVLYFSNSTFLGIRLIGNVFSMFIMAAFGMGIRSAIFIYLFRQFFKGLPIELEESAQV
                     DGAGVFRTFWSVMLPNARGAIVTVGLFAFVWQWNDYYFASLFQYSANNFAVFSTKLAG
                     GTDQLYTVLSGWVARGDSFFQDLTDETVRQNTLFYGLVANTAALLMMLPLLIGYFFVQ
                     KLFVESIERTGIVG"
BASE COUNT          517 a          434 c          453 g          735 t
ORIGIN      
        1 ggttatctgc ccaatttgtt ggcaaacaaa atcagttatc gcaaactgca agcgctgtgg
       61 ggctttgtgt ttgttctgcc cctcatcatc ggctttattt actttttctt attaccgttt
      121 ttagtcacca ttgtttatag tttttcccgc gtggaaaact taggattaaa ttcttcaacg
      181 ggtgaacata tcggtattgt ttcggaatgg attggcttcg ataattttac ttatatttta
      241 aaagagcact ccactttccg tcaaacccta accagttcgt ttaccacggc cttctttaat
      301 gttcccttga ttttaatttt tagtttaatt attgccgtgg ttctcaactc gaagtttaaa
      361 gggcgttcgt ttgtgcgggc catcttcttc atgccggtta tttttaactc gcaagcggtt
      421 gatgcggcca tgcaaaccgg agcccagtta gcagctgcca tggaaggcgc caccgccgat
      481 atttttgccc agatgtttaa tttccaagac tttttaattg atgctaatat cccggtaaat
      541 gcggtcaact tccttggtac gacggcggat cagatttatg atattatctc ttattccggg
      601 gtgcagatat taattttcct agcggggatt caatcggttc ctaaacattt gtatgaagct
      661 gccaaaattg aaggggcaac gcagtacgaa attttctgga aaattacttt cccgatggtc
      721 tcacccttaa tgttgacggc cggtgtttat accgtggtcg atagtttatt aaggtcacct
      781 ttaatcaata tgattaatgt ctttgcgacg gccggtgttc gaaaactggt taacggttta
      841 ggtgatgttt cttattatgg cattcatgcg tcgatgtcgt ggttattctg catttcctcg
      901 attatcatca ttgccatttg gttattctta ttatcgaagt tggtgttcta ttatgatgaa
      961 taaagacaac aaaactttga aattaggaac tttcaaacaa aaaatgcaga aaatgaaagc
     1021 ggattttaag gatccaacta aagtaaaagt tcgcagtcgc cgggccttta tggttaccgg
     1081 gagtgtgctt aggaccttta ttttaatcgg tttaatcttt gtgattttat tgcccttatt
     1141 ccaaaaattc tcgtttgccc tcaggcatcc gatggatatt accaatctgc aagttatttg
     1201 gattccggaa accatcagca atattaactt ccaaattgct tatcaattgc tcgatttcgg
     1261 acatacgatt tggaacacac tcattctgtc aacattttcc atgattattc agattatggc
     1321 gacggctgtt gccggctatg cctttgcccg cttaagattc aaagggagca atatcatctt
     1381 ttacatcgtg ctctttacct tggtggttcc caatgaaaca ttgcacattg ctcgcgtcct
     1441 gtacttttcg aattccacct ttttaggaat tcgtttaatc gggaatgtgt tttcgatgtt
     1501 tattatggcg gccttcggga tgggcattcg ctcggccatc ttcatttatt tattccggca
     1561 atttttcaag ggtttgccga ttgaattgga agaatccgcc caagtggacg gggccggagt
     1621 gtttcgcacc ttttggagcg tcatgctccc taatgcccga ggcgcgattg ttaccgttgg
     1681 tttgtttgcc tttgtctggc aatggaatga ttattatttt gccagtttat tccaatattc
     1741 ggccaacaac tttgccgttt tctcaaccaa attagccggt ggaacggatc agttgtatac
     1801 ggttttgtcc ggatgggttg cccgtggcga ttccttcttc caggacttga ccgatgaaac
     1861 tgttcgccaa aataccttat tctacggttt agtggctaac acggcagcgt tattgatgat
     1921 gttaccctta ttaatcggtt atttctttgt tcagaaatta ttcgttgaaa gcatcgaaag
     1981 aacgggcatt gtcggttaaa ccaagatgtc acgtttgcag cgtgacatct ttttagaaag
     2041 gaaacgacta tggaaatctc atcattaagc aagaagcaat tagtcggtca aatgctctgt
     2101 tttgcctttt taggaaccag ttttgatgaa caactgagg
//
LOCUS       DAOSGN010000134         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_51380_length_2135_cov_1.618269, whole genome shotgun sequence.
ACCESSION   DAOSGN010000134 DAOSGN010000000
VERSION     DAOSGN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51380_length_2135_cov_1.618269"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="PK087_02445"
     CDS             complement(<1..137)
                     /locus_tag="PK087_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018998710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOH18164.1"
                     /translation="MDKNSPIGVFDSGIGGLTVLESLKALMPQENFIYLADDAFYPYG
                     T"
     gene            complement(150..1133)
                     /locus_tag="PK087_02450"
     CDS             complement(150..1133)
                     /locus_tag="PK087_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18165.1"
                     /translation="MVIKEAYLDSEKLQIKAFLEKFDLNFDNDITETFFMEDNKEIIA
                     TISLSKHIIKCLAVSDEYQGEQLALKMVNQAVSFLHSKNEHFFQVFTKRIYRDFFLNM
                     GMRLIVESERISILESSSYPITLYLQDLKKQLALTTNNNGAIVVNCNPMTLGHLFLIE
                     QAIKNHQTLLLFVLQEDRSEFSTAERMALVKLGVAHLPNVIVAPSSPYLVSNLTFPSY
                     FLKEDSTRNNEYAYLDALIFKNYFMPILNITHRYIGTESHPMMVTYNNNLKLVLGKSL
                     TEIERIDYDGEPISASQVRKLIHNHQLYEALKLVPDNTRSLLSKIYYNRYK"
     gene            complement(1143..>2135)
                     /locus_tag="PK087_02455"
     CDS             complement(1143..>2135)
                     /locus_tag="PK087_02455"
                     /inference="COORDINATES: protein motif:HMM:NF016138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOH18166.1"
                     /translation="LGKNACGALGYAIPDLKFSKKVILVTDTVVDKIDNPEFPHSYVD
                     AVVVLPSIGEQKGIVSGTTKITTDPVGLKIARDAAKLLHELGMIKTGFSMQTGAGGTS
                     LAVADFVKKQMLAQNIVGSFASGGITAYYVQMLKLGLFEKLYDVQCFDLEAVTSYRDN
                     DHHFAMSSSEYGNPMEPNPIVNNLDFVILGATEIDLDFNVNVTTDSNDWLIGGSGGHS
                     DTAHGAKVTIITTNLIKSRMPIIKERVTTCTTPGEDVDVLVTERGIAINPKRQDLLVQ
                     LRNSRLNIKPIDELLTLCYQITSKPQVVVHDKKIIGVVKYRDGTIIDSLYQVKK"
BASE COUNT          707 a          406 c          347 g          675 t
ORIGIN      
        1 tttgtcccat agggataaaa agcatcatcc gccaaataaa taaaattttc ttgaggcatt
       61 aaagccttca aagattctaa aactgttaaa ccaccaattc ctgagtcaaa aactccgatg
      121 ggactatttt tatccataat atccttcttc tatttgtatc gattataata tattttactt
      181 aataaggagc gagtattatc cggaactaat tttaatgctt cataaagttg atgattgtga
      241 attaatttac gaacttgaga agcactgatc ggttcgccat cataatcaat tcgctctatt
      301 tcagtgagtg actttcctaa cactaatttt aagttattgt tataagttac catcatcggg
      361 tgagattcag ttccgatata acgatgggta atatttaaaa tgggcatgaa gtaatttttg
      421 aaaattaaag cgtccaaata agcatattca ttattccgag tactgtcttc ctttaaaaaa
      481 taactgggaa aagttaaatt agaaactaaa tatggtgagg acggagcaac aataacattg
      541 ggtaaatgcg caacccccag tttaactaaa gccattcgtt ctgcggttga aaactcagaa
      601 cgatcttctt gaagaacaaa taacaataat gtttgatggt ttttgattgc ttgttcaatc
      661 aaaaataagt ggcccaatgt catcggatta caattaacaa caatcgcacc gttattgttt
      721 gttgttaaag ctaattgttt ttttaagtct tgcaaataaa gcgtaatagg gtaagaactg
      781 ctttccaaaa tactgattcg ctctgattca acaatcaacc gcattcccat atttaagaaa
      841 aagtcacgat aaatgcgttt tgtaaaaact tgaaaaaaat gctcattttt actatgtaaa
      901 aaactgacag cttgattgac cattttcaaa gccaattgtt caccttgata ctcatcggaa
      961 accgctaagc acttaataat gtgcttgctt aaggaaattg ttgcaatgat ttccttatta
     1021 tcttccataa aaaaagtttc cgtaatatca ttatcgaaat taaggtcaaa cttttccaaa
     1081 aacgctttta tttgtaattt ttctgaatca agataagctt cttttattac catcattcca
     1141 ccttatttct tgacttggta aagggaatca ataatggttc catcacggta tttaaccacg
     1201 ccgatgattt ttttatcatg gacaacgacc tgcggtttcg aagtaatttg atagcataat
     1261 gtcaataatt catcaatcgg tttaatatta agacgagaat ttcgaagttg cacaagtaaa
     1321 tcttgacgtt taggattgat ggcaatccct ctttcagtta ccagaacatc cacatcttca
     1381 cccggagttg tgcaagttgt gactcgttct ttaataatcg gcattcgcga tttaattaaa
     1441 ttagtagtaa taatcgttac tttggcacca tgagcagtat cagaatgacc accactacca
     1501 ccaatcagcc aatcattgga atccgtcgtg acattaacat tgaaatctaa atcaatttcc
     1561 gtagccccca agatgacaaa atcgaggtta ttgacaatgg gatttggttc catgggatta
     1621 ccatattccg atgatgacat cgcaaaatga tgatcattat cacgataaga agttaccgcc
     1681 tccaaatcaa agcactggac atcatataat ttttcaaata gaccaagttt aagcatctga
     1741 acataataag cggtaattcc tcccgaagca aaactgccga caatgttttg ggcaagcatt
     1801 tgttttttta caaaatcagc aacggctaga gaagtccctc ccgccccagt ttgcatcgaa
     1861 aaacccgttt ttatcattcc taattcatga agcaatttgg ctgcatctcg agcaattttt
     1921 aaaccaacgg ggtcggtagt gattttagtt gttcccgaaa caatgccttt ttgttcacca
     1981 atggatggta aaacaacaac cgcatctaca taggaatgag gaaattccgg attatcaatt
     2041 ttatcaacaa ctgtatcagt cactaaaatg acttttttag aaaattttaa atcgggaatc
     2101 gcatacccca aggctccaca ggcattctta ccgag
//
LOCUS       DAOSGN010000135         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_51463_length_2132_cov_3.594126, whole genome shotgun sequence.
ACCESSION   DAOSGN010000135 DAOSGN010000000
VERSION     DAOSGN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51463_length_2132_cov_3.594126"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..871)
                     /locus_tag="PK087_02460"
     CDS             complement(173..871)
                     /locus_tag="PK087_02460"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18167.1"
                     /translation="MIDVHTHILPFVDDGSESLEESFRMIEREISLGITDIIITSHAL
                     RLDLKKVSKQELIDAFTQFYTLVQQKYDVRLYLGQEIAYSDKMISLLKNKELLTINNS
                     EYLLLELPYDEPINDIDEVIFSCRILGYKVIIAHVERYYYYDFKKLVELKKLGVLFQV
                     NSNSITGRSGKNIQKTALKLIKGKMVELVGSDVHAFRHNDMDEAFSLVSRRFGEEMAD
                     WLFSKNAKQLFNIL"
     gene            complement(877..1611)
                     /locus_tag="PK087_02465"
     CDS             complement(877..1611)
                     /locus_tag="PK087_02465"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOH18168.1"
                     /translation="MFDFKKFKKKNRLEQEYNIVVATKPNSAYAESFRKIPIDLKYSS
                     VDNEPRVIQITSSVAGEHKTTTATNLAAVYVELGKKVILVDCDLRKPKIHRAVEILND
                     DGLSSFLIDKVDKSQLIKKTKYNFDVINAGEVVPFPHVVLRSEKFQKLIKELRDMYDY
                     VILDCPPVLLVTDSLIISEVVDKTLFVINQQLTSKSETKEAITLLREANADLAGIILS
                     NVSRKVSNYGYRYGYRYKYRYYSRKE"
     gene            complement(1604..>2132)
                     /locus_tag="PK087_02470"
     CDS             complement(1604..>2132)
                     /locus_tag="PK087_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18169.1"
                     /translation="MSTADKVSENIKEFIKFKDIIEIVIEKENLPYKWHYIREHITTK
                     DIGAATAVTVTYEDADPVMARKIVIALANELARNINTKVPGQDPDALRFASETITLQN
                     TPDYNPNQEPSSPNKLLYYVISLLLGLIVGVGFIFLKETLSTRYNSKSEVERVLQLPV
                     IALIPQLENNGDKHV"
BASE COUNT          689 a          410 c          329 g          704 t
ORIGIN      
        1 tccggataat gttaaagtat aaacaggatc atctttttta catcctacta ataaaagagc
       61 caatacgccc aacaataaat atgaaatgat ttttttcatg tttttcctca cttcttcttt
      121 ccataacatt atactaaaaa tcagcggtta attcaaccgt ttttctaaaa tatcaaagaa
      181 tgttaaacaa ttgcttcgca tttttggaaa aaagccaatc cgccatttcc tcaccaaagc
      241 gacgagaaac aagggaaaaa gcttcatcca tatcattgtg acgaaaggca tgaacatcgc
      301 tgccaactaa ttcaaccatc ttccctttaa ttagtttcaa agctgttttt tggatgtttt
      361 tgccacttcg gccggtaata ctattgctgt taacctgaaa caagactcct aattttttta
      421 attccacaag ttttttaaag tcatagtaat aatatctttc cacatgagca ataatgactt
      481 tataaccgag gatgcgacaa ctaaaaataa cttcatcaat gtcattgatg ggttcatcat
      541 agggcaattc taatagtaaa tattcactgt tattgatggt caataattcc ttgtttttta
      601 ataatgatat cattttgtca gagtaagcaa tctcttgacc taaatataat ctcacatcat
      661 atttttgttg aacaagagtg taaaactgag tgaacgcatc aattaattcc tgcttggaaa
      721 ccttttttaa atcaagccgt agagcgtggg aagtaatgat aatgtccgta atacctaaac
      781 ttatttcccg ttcaatcata cgaaatgatt cttctaaact ttccgaacca tcatccacaa
      841 acggtaaaat gtgagtatgg acatcaatca tgaaaactac tcttttcttg aatagtatcg
      901 atatttgtaa cgatagccat atcgatagcc ataattagat actttgcgac tgacattact
      961 taaaatgatt cccgctaaat cggcattagc ttctcttaat aaggtaatcg cttctttagt
     1021 ctcactcttg gaagtaagtt gttgattgat aacaaacaaa gttttgtcaa caacttcgga
     1081 aataattaaa ctatcggtta ccaacaagac cggcgggcag tccaaaatga catagtcata
     1141 catatcacgt aattctttaa tcagtttttg aaatttttca cttcgtaaaa cgacatgggg
     1201 aaaaggcaca acttcgccag cattaataac atcaaaatta tatttagtct ttttaatgag
     1261 ctgtgatttg tcaactttgt caattaaaaa actcgaaagt ccatcatcgt tcaaaatctc
     1321 taccgcgcga tgaatcttag gttttcgtaa gtcacaatcg actaaaatga cctttttccc
     1381 taattccaca taaacagccg ctaaattggt agccgttgtc gttttgtgtt caccggcaac
     1441 cgaagaagtg atttgaatta ctcgcggttc attatcaacg ctggaatatt ttaaatcaat
     1501 cggaattttt cgaaaacttt ccgcataagc cgaattcggt ttagtagcaa caacgatgtt
     1561 gtactcttgt tctaaacgat tttttttctt aaacttttta aaatcaaaca tgtttgtcac
     1621 cattgttttc aagttgagga attaaagcaa tgaccggaag ttgcagaact ctttcgactt
     1681 cactttttga attataacgc gttgatagtg tttctttcag gaagataaaa ccaacaccga
     1741 caattaagcc caacaaaaga ctaataacat aataaagtaa tttattcggt gaagagggct
     1801 cttggttcgg attataatcc ggggtgtttt gtaaagtaat cgtttcggaa gcaaagcgta
     1861 aggcatccgg atcctgaccc ggaactttgg tattaatatt acgagctaac tcattagcta
     1921 aggcaatcac aattttccta gccatgaccg gatcagcgtc ttcataagta acagttaccg
     1981 ccgttgccgc gccgatatcc ttggtcgtaa tatgctctct aatataatgc catttatagg
     2041 gcaaattttc tttttcaata acaatttcaa taatatcttt aaatttaata aattctttaa
     2101 tgttttccga tactttatct gctgtgctca tc
//
LOCUS       DAOSGN010000136         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_51507_length_2131_cov_2.561657, whole genome shotgun sequence.
ACCESSION   DAOSGN010000136 DAOSGN010000000
VERSION     DAOSGN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51507_length_2131_cov_2.561657"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>2131
                     /gene="pnp"
                     /locus_tag="PK087_02475"
     CDS             21..>2131
                     /gene="pnp"
                     /locus_tag="PK087_02475"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020059142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOH18170.1"
                     /translation="MGELAKQANGAALVRFDDTAVLSAAVFSPKVSTGDFFPLMVLYQ
                     EKLYAAGKIPGGFFKREGRPSEFETLTSRLIDRPIRPLFADGFRNEVQVINTVLSSNP
                     DNSSQMAAMLGSSIALMISDIPFNGPIAGVVVGRVNGQFIINPTSAELEESDLNLIVA
                     GTEKAINMVESSAKEISEEEMLQALMFGHEEIKNLIAFQKTIQAAVGKPKVEVELHQV
                     PTDIDVAVRKMAEKDLLAAVVIKEKLARYARIDEINEQVIEHFTEQFKGDDEAEIKIN
                     YVKEVLETIVANEVRRLITDEKIRPDGRKIDEIRPLSSAVDVLERPHGSALFTRGQTQ
                     ALSVVTLGALGEFQIIDGLGVEEGKRFMHHYNFPQFSVGEVGRYGSPGRREIGHGALG
                     ERALLQVIPSEEDFPYTIRVVSEILESNGSSSQASICAGTMALMAAGVPIKAPVAGIA
                     MGLVSKDDKYTILTDIQGMEDHFGDMDFKVAGTKDGITALQMDIKISGVTEQILREAL
                     AQAKLGRLHILDHMMGTIAEPRQEVSKYAPKVLIINIDPDKIRDVIGSGGKIISDIIS
                     QCNNVKIDIEQTGRVSIMHQDLEWVDKAAKIIEDIVREAEVGAFYTGKVTRIEKFGAF
                     VELWPGCEGLLHISKIAAEKIAKVEDVLAIGDEIIVKVTEIDDKGRVNLARKEALEGR
                     KRPENSDGKRYSARPRRQYQD"
BASE COUNT          626 a          385 c          515 g          605 t
ORIGIN      
        1 agcggaaatt tgttgttgaa atcggtgaat tagccaagca agccaatggt gcggccttag
       61 ttcgctttga cgataccgct gtgttatcag cagccgtttt tagtccgaaa gtttccaccg
      121 gtgacttttt tcccttgatg gttttgtatc aggaaaaatt atatgcagcg ggaaaaattc
      181 ctggcggttt ttttaagcgt gaaggtcgtc cgtcagaatt tgaaactcta acttcccgtt
      241 taattgatcg gccgattcgt ccgctttttg cggatggttt tcgcaatgaa gtgcaagtaa
      301 tcaacaccgt attaagttcc aatcccgata attccagtca gatggcggcg atgctaggct
      361 catcaattgc cttaatgatt tcggatattc cgtttaatgg tcccattgcc ggtgttgttg
      421 ttggtcgtgt taatggtcaa ttcattatta acccgaccag cgctgaactg gaagaatccg
      481 atcttaactt aattgttgcc ggaacggaaa aagccatcaa tatggttgaa tccagtgcca
      541 aagaaatttc ggaagaagaa atgcttcaag ccttgatgtt tggacacgag gaaatcaaga
      601 atttaattgc tttccaaaaa acaattcaag cagcagtcgg aaaacccaaa gttgaagttg
      661 aactccatca agttccgacg gatattgatg ttgccgttcg caaaatggcg gaaaaagatt
      721 tattggcggc ggttgtcatt aaagaaaaat tagctcgtta tgctcgcatt gacgaaatca
      781 atgagcaagt cattgagcat tttactgaac aatttaaagg cgatgacgaa gccgaaataa
      841 aaatcaatta tgtaaaagaa gttttggaaa ctattgttgc caatgaagtt aggcgtttaa
      901 ttacggatga aaaaatccgc ccggatggtc gaaaaatcga cgaaattcga cctttatctt
      961 ccgccgtgga tgttttagaa cgtccgcatg gatcggccct atttactaga ggccaaactc
     1021 aagccttgag tgttgttact ttaggggctt taggagaatt ccaaatcatt gatggtttag
     1081 gagttgaaga aggaaaacgc ttcatgcatc attataattt cccgcaattt agtgtcggtg
     1141 aagttggccg ttatggttca ccggggcgtc gtgaaattgg tcatggagct ttaggagaaa
     1201 gagctttact gcaagttatt ccgagcgaag aagattttcc ttataccatt cgtgtggtca
     1261 gtgaaatatt agaatcaaat ggctcctctt cccaagcgtc aatttgtgcg ggtacgatgg
     1321 ctttgatggc tgccggggtg ccgattaaag caccggttgc gggaattgct atgggcttag
     1381 tttccaaaga tgataaatac accattttga cggatatcca aggcatggaa gatcattttg
     1441 gtgatatgga ttttaaagtt gccggaacta aagatggtat taccgcttta caaatggaca
     1501 tcaagatttc cggagttacc gaacaaatcc ttcgtgaagc tttggctcaa gccaaattgg
     1561 gaagacttca tattttggac cacatgatgg ggaccattgc ggaaccacgc caagaagtca
     1621 gcaaatatgc accaaaagta ttaattatca acattgatcc ggataagatt cgcgatgtta
     1681 tcggttcggg tggtaagatt atttctgaca tcattagtca atgcaataat gtgaaaattg
     1741 atattgaaca aacgggacgc gtttcgatta tgcaccaaga tttagaatgg gttgataaag
     1801 ccgctaaaat tattgaggat atcgttcgtg aagcggaagt tggggctttc tacaccggca
     1861 aagttacacg cattgaaaaa tttggtgcct ttgttgagtt gtggccgggt tgtgaaggtt
     1921 tattgcacat ttccaaaata gcggccgaaa agattgcaaa agttgaagat gtcttagcca
     1981 ttggggatga aataattgtg aaagtgacgg aaattgatga taaaggacgt gtcaatttag
     2041 cgcgaaagga agcccttgaa ggtcgaaaac gcccggagaa tagtgatggc aaacgctata
     2101 gtgctcgccc tcggcgtcaa taccaagata a
//
LOCUS       DAOSGN010000137         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_51548_length_2130_cov_2.426506, whole genome shotgun sequence.
ACCESSION   DAOSGN010000137 DAOSGN010000000
VERSION     DAOSGN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51548_length_2130_cov_2.426506"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PK087_02480"
     CDS             <1..558
                     /locus_tag="PK087_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOH18171.1"
                     /translation="AEIVAIPGLINVDFADVRTVMRNKGTALMGIGIASGPNRAVEAA
                     RKAIRSPLLEIDINGATDAIVFITSDVDIAMQEVVEVMTEIQNASSSEIDLVCGTGFN
                     MDLQGELIVTVIATGFDAKEIPEEEQANAGFPSYKPIEKEVKPTFVPEPSIEMGKPAD
                     SPQVEKKERIKGDTTIPAWLKNRFK"
     gene            775..1284
                     /locus_tag="PK087_02485"
     CDS             775..1284
                     /locus_tag="PK087_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOH18172.1"
                     /translation="MRNQTIRKLALASLFASMAIVLKTFLSIQLGESMRISLFPIPLL
                     VGGFLLGPWWGLLIAFVTDTVFFMISPFASFWSVYTFSTLIWGLAGGLIKLVPFKKDL
                     WKYIIVITITSIMETTINTLANFIYGLSNATLPWRILSMIIRIPLLVLATRIILKQFF
                     LLMDTNPFK"
     gene            1367..>2130
                     /locus_tag="PK087_02490"
     CDS             1367..>2130
                     /locus_tag="PK087_02490"
                     /inference="COORDINATES: protein motif:HMM:NF018155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase
                     (2,3-diphosphoglycerate-independent)"
                     /protein_id="HOH18173.1"
                     /translation="MSESSHKKPMMLVVMDGFGLSKTKVGNAIAAAKTPHLDYLLKNY
                     PHTTLGASGEDVGLPEGQMGNSEVGHLNIGAGRIVYQSLTRLNIAVRNDELDKNPAIN
                     KALVQAKANNGKLHIMGLLSDGGVHSHINHILYLLTKAVQFGIKEVVVHAFLDGRDVP
                     PQSATLYLKQLQDKINELGHSKIGVISGRYYAMDRDKNFDRLQLAYNALVYNDAMLKD
                     VFQGINESYSQGITDEFVVPYVASVDSNIEDGDSVI"
BASE COUNT          617 a          369 c          449 g          695 t
ORIGIN      
        1 gcggaaattg tcgctattcc cggtttaatc aatgttgact ttgccgatgt gcgcaccgtt
       61 atgcgtaata aaggaacggc attgatgggg attgggattg cctccggacc aaatcgggct
      121 gttgaagccg ctagaaaagc cattcgtagc ccgcttctgg aaattgatat taatggagca
      181 acggatgcca ttgtctttat caccagtgat gtggatattg ccatgcaaga agttgtggaa
      241 gttatgacgg aaattcaaaa tgcttcgtct tcagaaattg atttagtttg tggaacaggt
      301 ttcaatatgg acctccaagg tgaattaatt gtaaccgtga ttgccacggg tttcgatgcc
      361 aaagagattc cggaggaaga acaagctaat gccggtttcc ctagctataa accaattgaa
      421 aaggaagtaa aaccgacttt tgttccggaa ccaagtattg aaatgggaaa accagccgat
      481 tcgccccaag tggaaaagaa agaacgcatt aaaggcgata cgaccattcc cgcttggtta
      541 aaaaaccgtt ttaaataatg aggaaatcga ttctgatttc ctttttttta tggaataaga
      601 atattgggcg caaatttcgc cggtagtgct ttcttagcct ttaaactaat aaaagaacga
      661 atccctcctt tttacaatta agaaggaatt tgcctatttt tttgaaaaat attatcatta
      721 acaattgcct tgattagtga tataattatt tagtgaaaag gatattttga agatatgcgt
      781 aatcaaacta tccgtaaact tgctttagca tccttgttcg cttccatggc aattgtcttg
      841 aaaactttct tatcaattca acttggtgaa agtatgcgaa tcagtttgtt tccgattccg
      901 ctattggtgg gcggtttttt attggggccg tggtggggct tattaatcgc ttttgtaacc
      961 gataccgttt tttttatgat ttcccctttt gccagttttt ggtccgttta taccttttcg
     1021 acgctgattt ggggtttggc cggtggtctt atcaaactag ttccgtttaa aaaggatctt
     1081 tggaaataca taattgttat cacgattaca tcgattatgg aaactacgat taataccctg
     1141 gctaatttta tctatggtct ttccaatgcg actttaccat ggcggattct ttcgatgatt
     1201 attcggattc ctcttttggt cttagcgaca cggattattt tgaaacaatt tttcctgtta
     1261 atggatacta atcctttcaa atgagtgaat accttttcaa atcacaattc ttgaatttag
     1321 tcatgaaaag atgtataata attaaagaaa ttaaggagta gtattcatga gtgaatcaag
     1381 tcataaaaaa ccaatgatgt tagtggttat ggatggtttt ggtttatcaa agaccaaagt
     1441 cggaaatgcg attgccgctg ctaaaacccc tcatttggac tatcttttaa aaaactaccc
     1501 ccatacaact ctgggggcca gtggggaaga tgtcggttta cccgaaggcc aaatggggaa
     1561 tagtgaagtc ggtcatttga atattggcgc cggtcggatt gtttatcaat ccttgactag
     1621 attaaatatt gctgtccgca atgatgaact ggataaaaat cccgccatta ataaagcttt
     1681 ggttcaagcc aaagccaata acgggaaatt acatattatg ggtttattat ccgatggtgg
     1741 tgttcattcc cacattaatc atattttgta cttattaact aaagctgtcc aatttggcat
     1801 caaagaagtc gtcgttcatg cgtttttgga tggccgtgat gtcccaccgc aatcggcaac
     1861 gctttattta aaacaattac aggataaaat caatgaatta ggtcatagta aaattggcgt
     1921 tatcagtggt cgctattatg cgatggaccg tgataaaaat tttgatcgtt tacaattagc
     1981 ttataatgca ttagtttata acgatgcgat gttgaaagat gtttttcagg ggattaatga
     2041 aagttattcc caagggatta ccgatgaatt cgttgtccct tatgtggcct cggttgattc
     2101 caatattgaa gatggcgaca gtgtcatttt
//
LOCUS       DAOSGN010000138         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_51994_length_2117_cov_2.544132, whole genome shotgun sequence.
ACCESSION   DAOSGN010000138 DAOSGN010000000
VERSION     DAOSGN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51994_length_2117_cov_2.544132"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PK087_02495"
     CDS             <1..958
                     /locus_tag="PK087_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOH18174.1"
                     /translation="SEMNYPYAVGNIKAVESRLFDKSKFAKLARVEKSDFLKTLKELG
                     YAPSVTSDNLEDLINDELAALKEYFAEISPDPKLTDLFFMANDATNIKIMFKQRLFAT
                     EALHRYSHNGSINPNSLEKAIISQDYSEVAPEVKALLLDIEQQILPLSSARIISSKID
                     NAVYDYIFKHMGFSQARVLRKYYQALIDTTNVISWLRCRLLEWELSEFATMFIKHGLI
                     PLDVFIQSYAMNKDQVLRVFMDFYQEKITKALKAYFERPHLNQIEKQLDLLVLELMQE
                     EKNESFTIGPMIYYYLQKVAEAKNIRLLYSSVYFDPNDLLEY"
     gene            970..1287
                     /locus_tag="PK087_02500"
     CDS             970..1287
                     /locus_tag="PK087_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOH18175.1"
                     /translation="MNQKTEMAAIGLDETVLIYNALGIKTFVIKQADEADNIIFKLVN
                     QKFKIIFVSEAIYSHIPETLEKYQSLPFPMILPLPMEQASAGIGLKKIQDNVEKAIGI
                     NIF"
     gene            1302..>2117
                     /locus_tag="PK087_02505"
     CDS             1302..>2117
                     /locus_tag="PK087_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOH18176.1"
                     /translation="MSKKNLNEKVGIISKVSGPLVVAKNMKDVQMYDVVRVSEKKLIG
                     EVIELRGDMASIQVYEETAGIGPGEPVYFTNEPLSVELAPGLIEGIFDGIQRPLKLIY
                     DSSGDRIPLGIDVPKLDRSKVWDFKPLAKVKSHVESGDFLGEVEETTAVMHKVMVPQG
                     VSGKLSWIHSGKATIIDVIAKIETEQGLVEVKMLQTWPVRRGRPYARKLAPEAPMVTG
                     QRVIDTLFPIAIGGIAAIPGPFGSGKTVVQHQLAKWADADIIVYVGCGERGNEM"
BASE COUNT          633 a          392 c          474 g          618 t
ORIGIN      
        1 gagtgagatg aattatccct atgcagttgg caatattaaa gctgttgaaa gtcgcctttt
       61 tgataaaagt aaattcgcca aattggctcg ggtcgaaaaa agcgattttt taaaaacgct
      121 taaagaactg ggatatgctc cttccgtcac cagcgataat cttgaggatt tgattaatga
      181 tgaattggcg gccttgaagg aatattttgc tgaaatcagc cccgatccta aattaactga
      241 tttgtttttt atggccaatg atgcgacgaa tattaagatt atgtttaaac agcggctttt
      301 tgctacggaa gctctccatc gttattctca caatgggagc attaatccaa atagtttgga
      361 aaaagcgatt atttctcagg attattccga agtagcaccg gaagttaagg ccttgctttt
      421 agatattgaa caacaaatac ttcctttatc gagtgcgagg attatcagca gtaaaattga
      481 taatgcggtt tatgattata tttttaagca tatgggtttt tcgcaagccc gggtgttacg
      541 aaaatactat caagccctta tcgataccac caatgttatc agttggctgc ggtgtcgctt
      601 gttggagtgg gagcttagcg aatttgctac tatgttcatt aaacatggtc ttattcccct
      661 tgatgtcttt atccaaagtt atgctatgaa taaagaccaa gtgctgcggg tgtttatgga
      721 tttttatcag gaaaaaatca cgaaagccct caaggcttat tttgaacgcc cccatttaaa
      781 ccaaattgag aaacaacttg atttactggt gttggaattg atgcaggaag aaaaaaatga
      841 atcctttacc attggcccaa tgatttatta ctacctgcaa aaagttgcgg aggccaaaaa
      901 tatccgcttg ctttattcaa gtgtttattt tgaccccaat gatttattgg aatattaagg
      961 agactagcca tgaatcaaaa aactgaaatg gccgccatcg gccttgatga aacggtgtta
     1021 atttataatg ctctcggcat taaaaccttt gtcatcaaac aagccgatga agccgataat
     1081 atcattttca aactagtcaa tcaaaaattt aaaatcatct ttgttagtga agccatttat
     1141 tcccacattc cggaaacatt agagaaatac caatcgctac cgtttcccat gattctgcct
     1201 ttgccgatgg aacaagcttc ggccggcatc ggcttaaaga aaattcagga taatgtggaa
     1261 aaagcaattg gcattaatat cttttagaaa gggtgacgtc gatgagtaaa aagaatctaa
     1321 atgaaaaagt cggcatcata tcaaaggttt ccggaccgct ggtagttgcg aaaaatatga
     1381 aagacgtgca aatgtacgac gtcgttcgtg tcagtgaaaa aaaattaatc ggtgaagtca
     1441 ttgaactgcg tggggatatg gcttccatcc aagtatatga agaaaccgcc ggcattggtc
     1501 ctggcgaacc ggtttatttt acaaacgaac cgttgtcggt tgaattagca ccgggcttaa
     1561 ttgaaggaat ttttgatggt attcaaagac cgttaaaact tatctatgat tcatccggcg
     1621 atcggattcc tttaggaatt gatgtgccga aattggaccg aagcaaagtt tgggatttta
     1681 aaccgcttgc caaagttaaa agccatgtgg aaagcggcga ctttttaggt gaagttgaag
     1741 aaaccaccgc cgttatgcat aaagtgatgg ttccccaagg tgtttctggt aaattaagct
     1801 ggattcattc cggcaaggcg accattattg atgtgattgc gaaaatcgaa acggaacaag
     1861 gtttggtgga agttaaaatg ctacaaacat ggccggtccg tcgtggtcgt ccctatgcgc
     1921 ggaaattggc tcctgaagcc ccgatggtta ccgggcaacg agtgattgat actttgtttc
     1981 cgattgccat tggtggtatt gccgccatcc ccggtccatt cggctcaggg aaaaccgttg
     2041 ttcagcatca gttagcgaaa tgggccgatg ccgacatcat tgtttatgtc ggttgcggtg
     2101 aacgcggtaa tgaaatg
//
LOCUS       DAOSGN010000139         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_52117_length_2114_cov_2.078193, whole genome shotgun sequence.
ACCESSION   DAOSGN010000139 DAOSGN010000000
VERSION     DAOSGN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52117_length_2114_cov_2.078193"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK087_02510"
     CDS             <1..332
                     /locus_tag="PK087_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18177.1"
                     /translation="AVLDYEKIQGDITIRLGRSTDEYYGIGRGLLNVSHIPVYEDEVG
                     PFGSTTSDTLRTSITDSTSKILLFIICFSETFLDEHKQLAIDLFTKYAQGKNIKELDV
                     KRTSIEG"
     gene            335..1969
                     /locus_tag="PK087_02515"
     CDS             335..1969
                     /locus_tag="PK087_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003348006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOH18178.1"
                     /translation="MEEISNFIKTIMKNDLESGKAKKIISRFPPEPNAYLHIGHARAL
                     VTNFELAKEFGGYTNLRFDDTNPAKEDAEFVDAIIKDIEWLGYEPADVFFGSDYFEKT
                     YQLALGLIERGLAYVDDLSQEEIREYRGTLNEPGKESPYRNRSVAENLQLFTEMKDGK
                     YEEGSRVLRAKIDMAHPNINMRDPVLYRILYIHHHRQGDKWCIFPMYDFAHPIQDAIE
                     GITHSLCSLEYDNHRILYDWVVDNCFGPHDYPQQIEFGRLNITNTIMSKRYLKELVQK
                     GKVEDFDDPRMPTLVGLRRRGYTPEAIKKFILASGLSRINSTVEGSMLEHFLREDLKL
                     KTKRLMAVVNPLKVVIDNYEEGQVEFLEAANNVENADLGTRKMAFSKYLYIDKDDFVL
                     TKPDKHYKRLALGLEVRLYHAYFIKANSVEYNAHGEVSVVHCTYDPLTKSNTGFNARK
                     PNGTIHFVEATTAKKALFNLFEPLILDDDETKNFLDRINPKSWIQVEGVIENADEPFN
                     EGDKFQFIRDGYYAVDKVSRPEQLVFNRITSLKSGK"
     gene            1977..>2114
                     /locus_tag="PK087_02520"
     CDS             1977..>2114
                     /locus_tag="PK087_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOH18179.1"
                     /translation="MLRSLNEQQKAAVLATEGPVMVFAGAGSGKTRTLTYRIAYMIKE
                     KQ"
BASE COUNT          619 a          387 c          482 g          626 t
ORIGIN      
        1 tggctgttct tgattatgag aagattcaag gtgatattac catccgttta ggaaggtcca
       61 cggatgaata ctatggcatc ggtcgtggct tgcttaatgt ttcccatatt ccggtttatg
      121 aagatgaagt gggaccattt ggttccacca cttccgatac cttaagaaca agcattacgg
      181 attcgacttc caaaatttta ctatttatta tttgtttttc cgagaccttt ttggatgagc
      241 acaagcagct agcgattgac ttgtttacga aatatgccca gggcaaaaac ataaaagaat
      301 tagatgttaa gcgaacatca attgagggat gattatggaa gaaatatcaa attttattaa
      361 aacgattatg aagaatgatt tggaatcggg caaggcgaag aaaatcatta gtaggttccc
      421 accggaaccg aatgcttatt tgcatattgg tcatgctcgg gcccttgtta ccaattttga
      481 actagcgaaa gagtttggtg gttataccaa cctccgtttt gatgatacca atcctgccaa
      541 agaagatgcg gagtttgtgg acgccatcat taaagatatc gagtggcttg gttatgaacc
      601 ggcagatgtt ttcttcggct ccgattattt tgaaaaaact tatcaattgg cccttggttt
      661 aattgagcgg ggcttagcct atgttgatga tttgtcgcaa gaggagattc gtgaataccg
      721 tggcacttta aacgaacccg ggaaggaatc accttatcga aaccgcagtg ttgcggagaa
      781 cttgcaattg tttacggaaa tgaaagatgg caagtacgaa gaaggttcaa gagtattgcg
      841 tgctaaaatc gatatggcgc acccgaacat caatatgcgc gatcctgttt tatatcgcat
      901 tctatacatc caccatcatc gccaaggtga taaatggtgt attttcccga tgtatgattt
      961 tgcccatccg attcaagatg ccattgaggg gattactcat tctttgtgta gcctcgaata
     1021 cgacaatcac cgtattttgt atgattgggt cgtcgataat tgtttcgggc ctcatgacta
     1081 tccgcaacaa attgaattcg ggcgtttaaa tattaccaac acgattatga gtaaacgcta
     1141 tttaaaagag cttgttcaaa aaggtaaagt tgaagatttc gatgatccgc ggatgccgac
     1201 tttagttggt ttgcgccgcc gtggttatac gccggaagcc attaaaaagt ttattttggc
     1261 ttcgggtctt tcccgcatta attcgactgt tgaaggttca atgctcgaac actttttgcg
     1321 tgaagatttg aaactgaaga cgaaacgctt aatggcggtg gttaatcctt tgaaagttgt
     1381 tattgataat tatgaagagg gccaagtgga atttttggaa gcggctaata atgtcgagaa
     1441 tgccgatttg gggacaagaa aaatggcttt ttcgaaatat ttatatattg ataaagatga
     1501 ttttgtctta accaaaccgg ataaacatta taagcgctta gctcttggtt tagaggttcg
     1561 actgtaccat gcttacttta ttaaagccaa ctccgtggaa tacaatgccc atggggaagt
     1621 cagtgttgtt cattgcactt atgatccctt gactaaaagc aacacgggct ttaacgctcg
     1681 taagcctaac ggcaccattc attttgtaga ggccacaacg gccaaaaaag cgctctttaa
     1741 cttgtttgaa ccgctgattt tagatgatga tgaaaccaag aacttcttgg accgcattaa
     1801 tcctaagtct tggattcaag tagaaggtgt gattgaaaat gccgatgaac cgtttaacga
     1861 aggcgataaa ttccaattca tccgtgatgg ttactatgct gttgataaag tatcaaggcc
     1921 tgagcaatta gtttttaacc gcattacttc gcttaaatcc ggtaaatagg tgatttatgc
     1981 tgaggagttt aaacgagcaa caaaaagccg ccgttttagc aaccgaaggt ccggtaatgg
     2041 tctttgccgg ggcgggtagc ggaaagacga gaaccctaac ataccggatt gcttacatga
     2101 ttaaggaaaa acag
//
LOCUS       DAOSGN010000140         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_52154_length_2113_cov_1.994169, whole genome shotgun sequence.
ACCESSION   DAOSGN010000140 DAOSGN010000000
VERSION     DAOSGN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52154_length_2113_cov_1.994169"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="PK087_02525"
     CDS             <1..1313
                     /locus_tag="PK087_02525"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOH18180.1"
                     /translation="VMTFFIEKGVYDDNNVYQFIGGTGYPFWKFLTSPFRLFAGSDGL
                     KVIVISLFILILGGSFNVMNETHGIKALISFLIHKFKHRKYLLLYIVTLFFMLLGSLF
                     GIFEEAVTLLPIIIILALSLGWDTFTGLGMCLIATGFGFSTALTNPFTIGLASDQMGL
                     QILEGFWFRLFIFFLMYTLLCIFLTIHVKTIEKEPSKSPTYENDQKKLAIINPDESFE
                     NYDHRVLKIYAIFFLWVLLAIVLASTIPFLQNFTIPIIALSFLVGIFICGLLVGKTFK
                     EVGKLFWSGVLSLLPAVIMITLAASIKFILEDAQIMGTIIRQIVNWFTGSSPIMGILF
                     IYLIILLIQFFIGSASAKVILIIPIISIIANDLGITQNIALLAFIFGDGFTDLIYPTN
                     PVLLVALGISSFSYGKWLKKTILLQLVVLALTVGLLILGYYLGH"
     gene            complement(1396..>2113)
                     /locus_tag="PK087_02530"
     CDS             complement(1396..>2113)
                     /locus_tag="PK087_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH18181.1"
                     /translation="ANWQQTKQILVSKEFYRNYLYNTLDNTRLLMPNGEKYTVIGLVD
                     VGFEQNIYLHSSNLCELSLVLSSSTDSVIVYSLNKKATIKILEDEKLRARDRYQLSYE
                     EYYEGVFDIFQYIFSIIILASSLIFLYFIMRSSLIARVYEIGVYRALGVRKSNVYRLF
                     VSEIVLITLFSSLIGVIVASIFIVQVNKIAGMVLIEYPWFVGVASIIFLFICNLLIGL
                     SPVIHLLRSTPSQILSKYDI"
BASE COUNT          632 a          414 c          345 g          722 t
ORIGIN      
        1 atgtcatgac ctttttcatt gaaaagggcg tttacgatga taacaatgtt taccaattta
       61 ttggggggac gggatatcca ttttggaaat ttttgacttc accttttcgc ctgtttgccg
      121 gtagtgatgg tttaaaagtc atcgtcattt ccttgttcat cttaatcctt ggtggttctt
      181 ttaatgttat gaatgaaacc cacggcatta aagcgctcat cagtttccta atccataaat
      241 ttaaacatcg aaaatactta ttactctata ttgtcacctt atttttcatg ctccttggct
      301 ccttgtttgg catttttgaa gaagcagtga ccttgttacc gatcatcatt attttagctt
      361 tatcgttagg atgggacacc tttaccggtt taggaatgtg tctaattgca accggttttg
      421 gcttttccac agccttaacc aatcccttta ccatcgggtt ggcatccgac caaatgggcc
      481 tccaaatttt agaaggcttt tggtttcgcc tgttcatttt ctttttgatg tatacgcttt
      541 tgtgtatttt tctaaccatc catgtgaaga ccattgaaaa agagccaagt aaatcaccaa
      601 catatgaaaa tgaccaaaag aaattagcca tcattaaccc tgacgaaagt tttgaaaact
      661 atgatcaccg cgtattaaaa atttatgcca tcttcttctt gtgggtttta ttggccattg
      721 tcctagcatc cacgattccc tttttgcaga actttacgat tcccattatc gccctttctt
      781 ttttggtcgg catctttatt tgtggactgt tagtcggtaa aacctttaaa gaagtcggca
      841 aattattctg gtcgggagtt ctttcattat taccggctgt cattatgatt accttagcgg
      901 cttccatcaa attcatttta gaagatgccc aaatcatggg gactatcatc cgccaaatag
      961 tcaattggtt caccggttca tcaccaatta tgggaatctt attcatttat ttaatcattt
     1021 tactgattca attttttatt ggttccgcca gtgctaaagt cattctcatt attccgatta
     1081 tttccattat tgccaatgat ttagggatta cccaaaatat tgctttgtta gcctttattt
     1141 ttggcgatgg ctttaccgat ttgatttacc cgaccaatcc ggtcttatta gtggccctcg
     1201 ggatttcctc ctttagttat ggcaaatggt taaagaaaac catcttgtta caacttgtgg
     1261 ttttagcttt aactgtcggt ttactgattt taggatatta ccttggccat taaaaaacat
     1321 cttaaaagcc ccttgatttt tcagggggct ttttacatag ctcatataat aactattaaa
     1381 acttctatca tctttttaaa tatcatattt acttaaaatt tgcgatggag tggagcgtaa
     1441 caaatgaatt actgggctta aaccaattaa taaattgcaa atgaataaaa agataatcga
     1501 agcgacacca acaaaccacg gatattcaat gaggaccata ccggcaattt tattaacttg
     1561 cacaataaag atagaggcga caatcacccc aattaaggaa ctaaacaaag taattaaaac
     1621 aatttccgaa acaaataagc gatagacatt actcttcctc acccctaaag ctcggtaaac
     1681 gccgatttca taaacacggg caattaaact ggaacgcatg atgaaatata ggaagattaa
     1741 cgaacttgct aaaataataa tactgaaaat atattggaag atatcaaaaa caccttcata
     1801 atattcttca taactgagtt gatagcgatc acgagcccgt aatttttcat cttccaaaat
     1861 cttgatggta gctttcttat tcaatgagta aacaattacc gaatccgtcg aactacttaa
     1921 gacaagcgat aattcgcata aattactact atgcaaataa atattttgtt cgaaaccaac
     1981 atcaacaaga ccaattaccg tatatttttc tccgtttggc attagtaaac gggtattatc
     2041 aagcgtgtta taaaggtaat tacgataaaa ctctttgcta acaaggattt gtttcgtttg
     2101 ctgccaattg gcc
//
LOCUS       DAOSGN010000141         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_52529_length_2102_cov_3.488031, whole genome shotgun sequence.
ACCESSION   DAOSGN010000141 DAOSGN010000000
VERSION     DAOSGN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52529_length_2102_cov_3.488031"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PK087_02535"
     CDS             <1..892
                     /locus_tag="PK087_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOH18182.1"
                     /translation="CAFASCTNLVVVQFAQNSKLEYIGGSAFSECTSLLTIEIPGSVE
                     TIYNNAFGGCSKLGSVVFNEGLKWIASSAFKDCVELCNFDLPSSLLEIGDYAFRNCQG
                     ITKVEIPLAVSKIGKIPFEDCKNIASISVDENNTVYDSRSACNAIIITETNTLIVACK
                     NSTIPDGVVRIASCAFYKIDIKKVTIPASVKYIERLAFYLCNELPHDLIDRNNPKPFG
                     EVVTVFVPKTVETIECEAFVFNPGYGAGGSITGSHYCVHTDASSNEPPMPNWSTGQYG
                     YNWYRGGYIRFSNGALGWYW"
     gene            1086..>2102
                     /locus_tag="PK087_02540"
     CDS             1086..>2102
                     /locus_tag="PK087_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOH18183.1"
                     /translation="MEYRSWYERNIKTSLLGFGAMRLKTVNGEIDEAKGLALFDYAYK
                     HGINYYDTAMPYTEGKNETFVGKALKRYPRDSFYLASKLSLGMLNSQEEVLGFIDRQL
                     ENLQTSYLDMYLLHALNKERVKLIKEWDILTIVKRWQQEGKIRNIGFSFHDDYETFTE
                     ILAMNDWDFCQIQLNYMDTDIQQGIKGYEDLVERKIPVVIMEPIKGGKLATFSSSIAE
                     PFRRLSSASNASWALRWVGSLPGVKVMLSGMNEMEQLVDNLATCSSFQALTSLEYQAV
                     KEVKARLEKAIKVDCTNCRYCMPCTVGIDIPGNFRIFNSFGMYNKESDAHWQFNNLIR
                     HSTSL"
BASE COUNT          655 a          334 c          439 g          674 t
ORIGIN      
        1 atgtgcattt gctagttgta cgaatttagt cgtagttcaa tttgcgcaaa atagcaaatt
       61 agagtatatc gggggtagtg cctttagtga atgtactagc ttattaacaa ttgaaatacc
      121 gggttccgtt gaaaccatct ataataacgc ttttggtggt tgcagtaaat taggctccgt
      181 tgttttcaat gagggtctta aatggattgc cagttccgca tttaaagatt gtgtcgaatt
      241 atgcaatttt gatctaccgt catcattatt agaaatcggt gattatgcct ttagaaattg
      301 tcagggaata actaaagttg aaattccgtt agctgttagc aaaattggta aaattccctt
      361 tgaagattgt aaaaatattg ctagtatttc tgttgatgaa aataatacgg tttatgattc
      421 aagaagtgct tgtaatgcga ttatcataac cgaaaccaac actttaattg tagcttgtaa
      481 aaactcaacc attcctgatg gtgttgtaag aattgcttcc tgtgcttttt ataaaattga
      541 tattaaaaaa gtaacaattc cggcaagtgt taaatatatt gaaaggcttg ccttctattt
      601 gtgtaatgaa ttaccacacg atttaatcga tcgaaacaat ccaaaacctt ttggggaggt
      661 agttactgtt tttgtcccca aaactgttga aacaattgaa tgtgaagctt ttgtttttaa
      721 tccgggctat ggtgccggag gatcaataac tggttcacac tactgtgttc ataccgatgc
      781 ctcaagtaat gaaccaccaa tgcctaattg gtcaacgggt caatatggtt ataattggta
      841 tcgaggcggt tatatccgtt ttagcaatgg agctttagga tggtattggt aattttaatt
      901 aagagagcaa actgagttaa gtttactctc ttttgtcttg aatggtaaat taaactcagc
      961 tttcatcttt ggtataggca tcaaaatata ttgtaaagct actagaacga tatttaaagc
     1021 gttaaaggta aatttgctat cttaaaacaa aagaagtatg ataaaataaa gctagaggtg
     1081 agtttatgga atatcgaagt tggtatgaac gtaatattaa aacatcttta ctgggattcg
     1141 gagcgatgcg tctaaaaacc gttaatggtg aaattgacga agccaagggg ctggcgcttt
     1201 ttgattatgc ctataaacat gggattaatt actatgatac ggcgatgcct tatacggaag
     1261 gaaaaaacga aacctttgtt gggaaagcct taaaaagata tccacgagat tccttctact
     1321 tagcctccaa actttccttg gggatgctta attcccaaga ggaagtgtta gggtttattg
     1381 accgtcaact ggagaattta caaacctctt atttagatat gtatttgttg catgctttaa
     1441 ataaagaacg agtaaaatta ataaaggaat gggatatcct tacgattgtt aagcgctggc
     1501 aacaagaagg aaaaatccgc aacatcggtt tttcttttca tgacgactat gaaacattta
     1561 cagaaattct ggcgatgaat gattgggatt tttgccagat tcaattaaac tatatggata
     1621 ctgacatcca acaaggcatt aaaggttatg aagatttagt cgaaagaaag attccggttg
     1681 ttattatgga accaattaag ggcggaaaac tggcgacctt ttcatcgagt attgcggaac
     1741 cgttccgccg tttaagttcg gcctccaatg cttcatgggc tttaaggtgg gttggaagtc
     1801 ttcccggtgt caaggtgatg ttaagcggca tgaacgagat ggaacaatta gtggacaatc
     1861 tagcaacttg ttcttctttt caagcactga caagcttgga ataccaggcc gtaaaagaag
     1921 tcaaagctcg tttggaaaaa gcgattaaag tcgattgcac caattgccgt tattgcatgc
     1981 cttgcaccgt cggtattgat attcccggta attttcgcat tttcaattct tttggcatgt
     2041 ataataaaga aagtgatgcc cattggcagt ttaataattt aatccgtcat tctacttcgc
     2101 ta
//
LOCUS       DAOSGN010000142         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_52617_length_2100_cov_2.577506, whole genome shotgun sequence.
ACCESSION   DAOSGN010000142 DAOSGN010000000
VERSION     DAOSGN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52617_length_2100_cov_2.577506"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1531)
                     /locus_tag="PK087_02545"
     CDS             complement(134..1531)
                     /locus_tag="PK087_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOH18184.1"
                     /translation="MMYHKINNQDLQYFKSFLPEERILTGTAISEDYSKDEMGTIVAH
                     PEVVIKVLSTEEIAAIFKYASEKLIPITVRGSGTGLVGACVPLYGGIVLDTTLMNRIL
                     ELDEENLVLVVEPGVLLMDIYSFVEPKGYFYAPDPGEKSATIGGNIATNAGGMRAVKY
                     GVTRDWVRAIEVVLPSGEITQLGRKVVKDTTGYSLKNLIIGSEGTLAVITKAYLKLSP
                     LPSKTVSLLVPFKTLEQGIDQVPHILQSRSDPTAVEFFGRETIGFAEMFLGKKFPDPH
                     NQAYILLTFDGQDDKDVKAKVERAADLCLNELGAEDVLIVDTEERKDLVWKARGAFLE
                     AIKASTTQMDECDVVVPRAKVSEFVKFTYDLTKKYQVRIPSFGHAGDGNLHLYICKDD
                     MADDQFADVLEKVFAEMYNQADQMGGLISGEHGIGFAKKKYMQHLLGPTQMALMQGIK
                     KVFDPNNILNPGKIV"
     gene            complement(1528..>2100)
                     /locus_tag="PK087_02550"
     CDS             complement(1528..>2100)
                     /locus_tag="PK087_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOH18185.1"
                     /translation="GGNIMASIICPNNRPQMATVRYKIFKKPDQVKPTGKIVKMKTAG
                     VDFTSGLSLLEISPKPKEMDISEADTLVACGRPFKTPEQLALAYELADLLGAQIACTR
                     PLIEAGLIDPRKQIGLSGRTVAPKLLITLGVSGAVQFTAGMRGSELVFAICDDAKAAI
                     FDVAHYGVVGDVFAILPALIKKIKAAKGLI"
BASE COUNT          586 a          496 c          412 g          606 t
ORIGIN      
        1 aaacgttcag aaatggacgt tttctgtttt gaggaagtgg caaactcggg ttatagcaca
       61 aaaagaacaa atgtgtctat ctttttattg aaaaatcaac aaaagaactg tgtttttata
      121 atattattct tgattaaaca attttcccgg gatttaaaat gttgttaggg tcaaaaactt
      181 ttttaattcc ctgcattaaa gccatttggg ttgggcctaa taagtgctgc atgtattttt
      241 tcttagcaaa accaatgcca tgttctccgg aaataagtcc acccatttgg tcggcttgat
      301 tatacatttc cgcaaaaact ttttcgagga catcagcaaa ttggtcatca gccatatcat
      361 ctttacaaat atacaaatgg aggttgccat caccggcatg accgaagctc ggaatgcgga
      421 cttgatattt cttcgttaaa tcgtaagtga atttcacaaa ctcactaact ttagcccgtg
      481 gaacaacgac atcgcattca tccatttgcg tggtggaagc tttaatggct tccaagaaag
      541 ccccgcgggc tttccaaacc aaatcttttc gctcttcagt atcgacaatt aaaacatctt
      601 cggctcccag ttcatttagg cataaatcag ccgcccgttc gactttagcc ttcacatctt
      661 tatcatcttg accgtcaaag gttaataaaa tataggcttg gttgtgggga tcgggaaatt
      721 tctttcctaa aaacatctcc gcaaagccaa tcgtctcccg cccaaagaat tcaaccgctg
      781 ttggatcact gcgggattgt aaaatgtggg gaacttggtc gatgccttgt tccaaggttt
      841 taaacggcac cagcaaactg acggttttgc ttggtaaggg tgataatttt aaatacgctt
      901 tagtaatgac ggccaaggtt ccttcgctac cgatgattaa attctttaag gaataaccgg
      961 tggtgtcttt cacaaccttc ctccccaatt gcgtaatttc acccgatggc aaaaccactt
     1021 caatcgctcg aacccaatca cgggtaacac catatttgac cgcccgcatc ccgccggcat
     1081 tagtggcaat attgccaccg atggtggcgc ttttttcccc ggggtcagga gcatagaaat
     1141 aacccttcgg ttcaacgaaa ctataaatat ccatcagtaa gacgcccggt tccaccacta
     1201 gcactaagtt ttcctcatcg agttctaaaa tgcggttcat aagggtggtg tctaaaacaa
     1261 tccctccata taaaggcaca caagcgccaa ctaaaccggt accactacca cggacagtaa
     1321 tcgggatgag tttttcacta gcgtatttaa agatggcagc aatttcctcc gtcgacaata
     1381 ctttaataac tacttccgga tgggcaacaa tcgtccccat ttcatctttg ctgtaatctt
     1441 cgctgatggc agtccctgtt aatatccttt cttcgggaag aaaactttta aaatactgta
     1501 aatcttgatt attgatttta tgatacatta tattaatcct ttcgcggctt taatcttctt
     1561 gattaaagcg gggagaatgg caaagacatc gccaaccact ccataatgag caacatcgaa
     1621 gatggctgct ttcgcatcat cacaaatggc aaaaactaat tcgctccccc gcatgccggc
     1681 cgtaaattgc acggctccac taacgcctaa agtaattaac aatttcggcg caacagtgcg
     1741 accgctcaag ccgatttgtt tacggggatc aattaagccg gcttcgatta agggtcgcgt
     1801 acaagcgatt tgtgccccta ataaatcagc taattcataa gcgagggcca attgttccgg
     1861 cgttttaaac ggtcgcccac aagcaacaag cgtatcggct tcactaatgt ccatctcttt
     1921 gggtttgggg gaaatttcaa gcaagcttaa tccgcttgtg aaatccacgc ccgcagtctt
     1981 catttttaca atcttgcccg tgggttttac ctgatccggc tttttaaaga ttttataacg
     2041 gaccgtcgcc atttgggggc ggttgttcgg gcaaataatg ctcgccatga tattaccacc
//
LOCUS       DAOSGN010000143         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_52974_length_2090_cov_2.646192, whole genome shotgun sequence.
ACCESSION   DAOSGN010000143 DAOSGN010000000
VERSION     DAOSGN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52974_length_2090_cov_2.646192"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PK087_02555"
     CDS             <1..1257
                     /locus_tag="PK087_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOH18186.1"
                     /translation="FFINNRIRLGKLMKDNSLSVFFSGRKIQKSADQDFPFEVDKNFY
                     YLTGLNQANIVLTLFKKQNDLKATLYIEENDPILVKWVGIKYTKEEAALISGVNQVVY
                     ANLFDNHLFAYFNNSRYQSDCVRHVYLNLERSSLPGYTNQALEFANLTMTAKYPEVTV
                     INAYEMMVGLRMIKSEEEIALVKSSLKTTKKGIESLMSHAQAGLYEYQLEAYFDWQVK
                     CDGNRDLAFKTIAASGKNATILHYEDNNSLLKNDNLVLFDLGARTEFYVSDITRVFPV
                     SGKFTARQKAVYDAVLDVNKRCIAYLKPGLSWAEFNKFAKDLLTQHAYELGLIKEDQE
                     ISKYYYHSIGHSLGLDTHDPTLYDWGIKEGMIITVEPGLYIEEEGIGVRIEDNVLITD
                     EGCENLSSEIIKETADIESFMATYKK"
     gene            1314..1949
                     /locus_tag="PK087_02560"
     CDS             1314..1949
                     /locus_tag="PK087_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18187.1"
                     /translation="MKKLLIFTLFLWIFLGLDLYAYSPTGHENVSVVEFIDDGELLID
                     MTSSQIEAGYKTLGRNRLFGWKHYFFNIKEEANYVGEIIFSRSNRTTEKLKIDYELKE
                     THYSERSIKTTGSLSADFEGKIKKIDAGLKASASVTSDKKTAVTRVEDTSFSFVVDPF
                     HRMTFRVTGEALVTNGVSKLSILGMTFKKGTWEYIDIITRYYELYEEELPR"
BASE COUNT          686 a          316 c          400 g          688 t
ORIGIN      
        1 ttctttatca ataatcggat tcgtttggga aaattaatga aagataattc cttatctgtt
       61 ttcttttctg gtcgaaaaat ccaaaaatcg gctgatcaag attttccttt tgaagtcgat
      121 aaaaattttt attatttaac aggccttaat caagccaata ttgttttgac tctttttaaa
      181 aaacaaaatg atcttaaagc tactttatac attgaggaga acgatcctat tttagtaaag
      241 tgggtgggaa taaaatatac aaaagaagaa gcggccctaa tatccggagt taatcaagtt
      301 gtttatgcca atttatttga taaccatctc tttgcttact tcaataattc ccgttatcaa
      361 tccgattgtg ttaggcatgt ttatttaaac ttagaaagaa gtagtttacc gggctatacc
      421 aaccaagctt tggaatttgc caatttaacg atgacggcga aatatccgga agttactgtc
      481 attaacgctt atgaaatgat ggtcggatta cgtatgatta aatcggaaga ggaaattgca
      541 cttgtaaagt ccagcctcaa aaccactaaa aaaggtattg aaagccttat gagtcatgca
      601 caagcgggtt tatatgaata tcaattagaa gcttattttg attggcaggt taagtgcgat
      661 ggcaaccgcg atttagcctt taaaacaatt gccgcttccg gaaaaaatgc caccatttta
      721 cattatgaag acaataatag tttacttaaa aatgacaatt tggtattgtt tgatttagga
      781 gctcgaacgg aattttatgt tagcgacatt acacgagtgt tcccggtttc cgggaaattt
      841 actgctcgcc aaaaagctgt ttatgatgct gttcttgatg tcaataaacg ctgtattgcg
      901 tatttaaaac cgggactttc ttgggctgaa tttaataagt ttgcgaaaga tttacttact
      961 caacatgcct atgagttggg attaatcaaa gaagatcaag aaatcagtaa atattattat
     1021 cattccattg gtcattcttt aggattagat acccatgacc cgaccttata tgattgggga
     1081 ataaaagaag ggatgattat tactgttgaa ccgggattat acattgagga agaaggaatt
     1141 ggggtaagaa ttgaagacaa tgtcttaatt accgatgaag gctgcgagaa cttaagttcc
     1201 gagattatta aggaaaccgc tgatattgaa agttttatgg ctacttataa aaagtagcca
     1261 ttttttaacg gaattttttc tccgcttaat aatatatatt tggaggtaat aaaatgaaaa
     1321 agttgttgat atttacttta ttcctttgga tttttttagg cttagattta tatgcctatt
     1381 ctcctacggg tcatgaaaat gtttcagttg tcgaatttat cgacgatggt gaattattga
     1441 ttgatatgac ctcttcgcaa attgaagctg gttataagac attaggtaga aatcgtctat
     1501 ttggttggaa acattatttt tttaatatca aggaagaggc aaattatgtc ggggaaatta
     1561 ttttttcgcg cagtaatcgg acgacggaaa aattaaaaat tgactatgaa ttaaaagaaa
     1621 cgcattattc tgaacgtagc atcaaaacca cgggttcttt atccgctgat tttgaaggga
     1681 aaattaaaaa aattgatgcg ggattaaaag catcagcttc agtaacaagc gataaaaaaa
     1741 cggctgttac acgagtggaa gatacgagtt tttcttttgt tgttgatccg tttcatcgaa
     1801 tgacttttcg agtgacggga gaagcattag taaccaacgg cgtcagtaaa ctttccatcc
     1861 tcgggatgac ttttaaaaaa gggacttggg aatatattga tatcattacc agatattatg
     1921 aactatacga agaagagttg ccacgatgaa aaaactaaat tatttaattc tctttttcgt
     1981 aagcacttta atcatcatag gcttaatttc tctttattct aaacaagaca aagtttttca
     2041 aatgacggtc attcctcaag ctttcagtct tgtttcggcc actaatgacg
//
LOCUS       DAOSGN010000144         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_53015_length_2089_cov_2.694690, whole genome shotgun sequence.
ACCESSION   DAOSGN010000144 DAOSGN010000000
VERSION     DAOSGN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53015_length_2089_cov_2.694690"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PK087_02565"
     CDS             <1..1026
                     /locus_tag="PK087_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH18188.1"
                     /translation="CSAKNAEKGLCNPQTNYNVGRINDPNEQYLFFDSAMVPYEYNGG
                     YYALPNTQSFLVMFVRNDIFEARGWTVPETWREVTSLVTELSISNLQFYLPVSASGAT
                     SVVNQVFASMLYQKGGTFYRNNNKESNFDTEEAMQAFEEWCKYYTDYSFPLAASFLNR
                     FRTGETPIGIANYELFNTLTVFAPEISNKWSYAPLPGTYNDKNQLINNGAASGSAIVM
                     MKQADDKESAWDFMRWWVSRDTQTSYARELESILGAAARHNTANVLAFKNLPWSREEQ
                     NVLLAQWKNSVGVPEIPGGYYTGRNLENAFRKVVNTQANPRETLNEYIALINAEITKK
                     RKEFGLD"
     gene            1040..2038
                     /locus_tag="PK087_02570"
     CDS             1040..2038
                     /locus_tag="PK087_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH18189.1"
                     /translation="MNDNNVSTSIKKPSLWSRFRAWRIKKSIEIDHWSRKKPFRRKMY
                     LNRSLYAMMAPYLIMFTCFTVAPVVMSFMLGFTYFNMLEAPRWVGWQNYIDLFLNDPI
                     FITAIKNTLIIALITGPLGYIMAFVFAWLINELPQKLRAIITLVFYAPSIAGGAITIW
                     ALIFASDIYGYANAYLTDWGIIHEPITWLQNATYILPIVIIVQLWMSLGVNFLSLIAG
                     LKGIDRNQYEAGAVDGIKNRWQELWYITLPNMKSILIFAAVIQISGALGVGAVTTALA
                     GFPSVAYAGHTIVNHLQDYGSIRYEMGYASAIATILFFSAILLNRLIRRIIRKVGS"
BASE COUNT          569 a          444 c          462 g          614 t
ORIGIN      
        1 tgttcggcta agaatgcgga aaagggttta tgcaatccgc agacgaatta taatgtcggt
       61 cgcatcaatg acccgaacga acaatatttg ttctttgata gtgccatggt cccttatgaa
      121 tataatggcg gctattatgc actgccgaat acgcaatcct tcttagtcat gtttgttcga
      181 aatgatattt ttgaagcccg aggctggacc gttccggaaa catggcggga agtaacttct
      241 ttagttacgg aactatcgat ttccaatctc cagttttatc ttccggtttc cgcttccggg
      301 gccaccagtg tcgttaacca ggtctttgct tcaatgttat atcaaaaagg cggaactttc
      361 tatcgtaaca acaataaaga atccaatttt gacaccgaag aagcgatgca agcttttgaa
      421 gaatggtgta aatattatac cgattacagt ttccctttgg cggcgagttt cttaaaccgc
      481 ttccggaccg gggaaacacc gattggcatc gccaactacg aattatttaa tacgctaacc
      541 gtttttgctc cggagatttc caacaaatgg agttatgctc cgctgccggg cacttacaat
      601 gacaaaaacc aattaatcaa taatggcgcc gcttccggaa gcgcaattgt tatgatgaag
      661 caagccgacg acaaggaatc cgcttgggac tttatgcggt ggtgggtatc gcgtgacacc
      721 caaaccagct atgccagaga actggaatcc attttagggg cggccgctcg tcacaatacc
      781 gccaatgttt tagcatttaa gaatttacca tggtcgcgag aggaacaaaa tgttttgcta
      841 gcacaatgga aaaacagtgt tggcgttccg gaaattccgg gtggttatta caccggccgt
      901 aatttagaga atgctttccg gaaagtcgtt aatacgcaag ccaatccgcg ggaaacactc
      961 aatgagtata tcgccttgat taatgcggag attaccaaaa agcgtaaaga atttggttta
     1021 gattaaggag acgaacaaga tgaatgacaa taatgtttct acatccataa aaaaaccatc
     1081 cctttggagt cgtttccgag catggcgtat taagaagagc attgaaatcg accattggag
     1141 tcgaaaaaaa ccgttccgcc ggaaaatgta tttaaatcgt tcgctctatg caatgatggc
     1201 tccttatctg attatgttca cctgttttac ggtcgctcca gttgttatga gctttatgtt
     1261 aggctttacc tactttaaca tgttggaagc gccgcgctgg gtgggctggc agaattacat
     1321 tgatttgttt ttaaacgatc cgatttttat tacggcgatt aaaaacacct tgattatcgc
     1381 tttaattacc ggacctttag gttatatcat ggcctttgtc tttgcttggc tcattaatga
     1441 attgccgcaa aaactgcggg ccatcattac cttggttttc tatgccccgt cgattgccgg
     1501 gggtgcgatt accatttggg cacttatttt cgcaagtgat atctacggtt atgccaatgc
     1561 ttatttaacc gattggggaa ttattcacga accgattacc tggttgcaaa atgcgactta
     1621 cattttaccg attgtcatta ttgtgcaact atggatgagt ttaggagtta acttcctttc
     1681 cttgattgcc ggattaaaag gcattgaccg caaccaatac gaagccggtg ccgttgacgg
     1741 cattaaaaac cgttggcagg aactttggta tattacttta ccgaacatga agtcgatttt
     1801 aatttttgcg gcggtcattc aaatttccgg agccttgggt gttggggcgg tcacgaccgc
     1861 actggccggc tttccgtccg tcgcctatgc cggtcatacc attgtcaatc atttgcagga
     1921 ctacggttcc atcaggtatg aaatgggcta tgcctctgct attgcaacga ttctcttctt
     1981 ctctgcaata ttattaaatc gtttaattcg gagaattatc agaaaggtgg gttcataatg
     2041 gctaaagtta aaaaaacaaa agatccttcc caaaaagctc tcaccttaa
//
LOCUS       DAOSGN010000145         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_53465_length_2078_cov_2.288680, whole genome shotgun sequence.
ACCESSION   DAOSGN010000145 DAOSGN010000000
VERSION     DAOSGN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53465_length_2078_cov_2.288680"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /gene="glgP"
                     /locus_tag="PK087_02575"
     CDS             complement(<1..1532)
                     /gene="glgP"
                     /locus_tag="PK087_02575"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HOH18190.1"
                     /translation="MNKMELREIVEDNLSKMYGVTLTDANIEQLYKASAVSVNDILRK
                     KRRVYNNIVKENQGKRVYYLCMEFLMGRSFKNNIYNLGLVDEFSSILESIGYTLDDLY
                     EHEPDAGLGNGGLGRLGACFLDALASQAYPAMGFSIRYEYGLFKQKIVNGWQTELPDV
                     WLPGGEVWLTLRGDKVYQIRFNGEVIEKHVGDKIIYDYQNYKAIEAVPYDMMVSGANS
                     EAISVLRLWRSRNIRDFNLNLFSQGDYAQAMKEYNEADLISKVLYPSDDHYEGKALRV
                     KQQYFLVSASMQNIVADHLRYYGDIHSLPKYVAIHINDTHPALCIPELMRILIDEYDL
                     SYEESWEIVQKTISYTNHTVMSEALEKWSEELIQKLLPRIYIILKQINERFIHYCHEL
                     VLSDAAIADLAIIHHGEIRMANLSVVASFKVNGVSKLHSDIIKNDLFKGFAQIWPEKF
                     TNVTNGIAYRRWLNQANPRLVELLNDKIGTAYMQNAEELEKLLAYQDDDDAERKSCYK
                     PI"
     gene            complement(1558..>2078)
                     /locus_tag="PK087_02580"
     CDS             complement(1558..>2078)
                     /locus_tag="PK087_02580"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOH18191.1"
                     /translation="GMKLFSKVKEILGEVQIIAEDLGFLTAEVYQLLHDCGYPGMKIL
                     QFGFDARSDSEYAPHNYVKNAVVYTGTHDNLPIEAWIDSLNPKDYQYFMDYLHLNDRK
                     KAGFTAILECLKSVCEIAIIPMQDYLALKGEARMNRPSFLGGNWIWRVQKAAMNEQLA
                     KKINYWVKVYRR"
BASE COUNT          624 a          456 c          370 g          628 t
ORIGIN      
        1 aagattggtt tgtagcagct tttccgctct gcatcatcat catcttgata ggctaagagt
       61 ttctctaatt cctcagcatt ttgcatgtaa gcggtgccaa ttttatcatt caaaagttcc
      121 accaaacgcg gattggcttg attgagccag cgacggtagg caatgccatt ggtgacattg
      181 gtgaattttt ccggccaaat ttgtgcaaag cctttaaaga gatcattttt aataatatca
      241 ctatggagtt tcgagacccc atttacttta aaggaagcca caaccgaaag attagccatc
      301 cgaatttcac catgatggat gattgctaaa tcggcaatgg ccgcatccga aaggaccaac
      361 tcgtgacagt agtgaataaa tctttcattg atttgtttta aaataatata aatgcgcggt
      421 aaaagttttt gaattaattc ttccgaccat ttttccaaag cttcggacat aacggtatgg
      481 ttagtataac taatggtctt ttgaacaatt tcccagcttt cctcataact taaatcatat
      541 tcgtcaatta aaatccgcat taattcaggg atgcaaagcg ccgggtgggt atcattaatg
      601 tgaatcgcca catatttagg aagactatga atgtctccat aatagcgtaa gtgatcggca
      661 acgatgtttt gcatcgacgc actaactaaa aaatattgtt gtttaacccg taaggcttta
      721 ccctcataat gatcatcgga agggtagaga actttcgaaa ttaaatcggc ttcattatat
      781 tccttcatcg cttgtgcgta atcgccctgc gaaaacaaat taaggttaaa atccctaatg
      841 ttacggctcc gccataagcg caaaacactg atggcttcgc tgttggcgcc ggaaaccatc
      901 atatcataag gcaccgcttc aatcgcttta taattttgat aatcataaat gattttatcg
      961 ccaacatgtt tttcaatgac ttcaccgtta aacctaattt gataaacttt atcaccccgt
     1021 aaggttaacc aaacttcgcc cccgggaagc caaacatcgg gcaattccgt ttgccagcca
     1081 ttaacaattt tttgtttgaa caaaccatat tcataacgaa tcgaaaagcc catggccgga
     1141 taggcttgtg aggccaaagc atctaaaaag catgctccta aacgacctaa accgccattg
     1201 cctaagccgg catccggttc atgttcgtat aaatcatcca aagtatagcc aatgctttcg
     1261 agaatgctgc taaactcgtc cactaagcct aaattataaa tattgttttt gaaggaccgc
     1321 cccatgagaa attccatgca caaatagtaa actcgtttcc cttggttttc ttttacaata
     1381 ttgttataaa cgcgccgttt ttttcgtaaa atatcattta cggaaaccgc ggaagcttta
     1441 taaagctgtt caatattggc atccgtcaag gtcaccccat acatttttga taaattgtct
     1501 tccacaattt cccttaattc cattttattc ataatgcctc cttgtgggta agtacgatta
     1561 tcgacgatat actttgaccc aataattaat ttttttggct agttgttcat tcatagccgc
     1621 tttttggaca cgccaaatcc agttgcctcc taaaaaggag ggtcggttca tccgggcttc
     1681 acctttcaaa gctaaataat cctgcatcgg gatgatggca atctcacaaa cggattttaa
     1741 acattccaaa atggctgtaa aacccgcctt ttttcgatca ttaagatgta aataatccat
     1801 aaagtattgg taatctttag ggtttaaact atcaatccac gcttcaatcg gaagattatc
     1861 atgcgtgccg gtgtaaacaa cggcattctt cacatagtta tgcggagcat attcactgtc
     1921 actgcgggca tcaaagccga attgaagaat tttcatgccc ggataaccac agtcatggag
     1981 aagttgataa acttcggccg tcaaaaaacc caagtcctca gcaataatct ggacttcgcc
     2041 caagatttct ttgaccttgg aaaataattt cattcccg
//
LOCUS       DAOSGN010000146         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_54116_length_2061_cov_2.674975, whole genome shotgun sequence.
ACCESSION   DAOSGN010000146 DAOSGN010000000
VERSION     DAOSGN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54116_length_2061_cov_2.674975"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2061
                     /locus_tag="PK087_02585"
     CDS             <1..>2061
                     /locus_tag="PK087_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOH18192.1"
                     /translation="AEFDPITWTISECHKRGIEFHAWLNPYRLGSSTLGLSVEDYAKT
                     FPSYNVASNPAYILKGTTGAILNPGEISVRKFIIDSCMEIIENYDVDAIHFDDYFYIG
                     GVDDSATRAKYNFNNLPIPDFRRQQVDLFIEALSSKMREYNQLNNRHVQLGISPTGIY
                     RNGSYQATATYDAKGSLITPVGSNTSGFAHYGDYLYCDTKKWVDNEWIDYILPQSYWS
                     FEEPAAAYADVMDWWVKVVKNKKVNLYSGMGIYQAAEAKRASWNTNVNEAGNQVQYAS
                     QYPEIKGHCLFSYSQVQSAYLTKSGIFYNNLNRIRQLMWTNKAVLPEIETFTSIKLSP
                     VTNLAISKNDQGFRLDFDKQTKAKTYVIYRSPRPITYAPNEIIDIIGNEFIDNRISYF
                     DKTNSSQNYYYALKVQSATNTLSEGVEINTTQATSGASMLRLEPFENVFFSDYNYFNG
                     FLTVRWDEIYPLFGGKPSYEVYYSPDNINFQLLNTTTYPLEDNGGYYQTRIPLDNSPT
                     VYVYLKAFNDYGSVVSETEALTITKRIGHIYNLTVRGDAFAGEMITLMWNKLKLTDVN
                     YTAQYSIDGFNWHNITSVDNPLVEEGQNCYQPFILPKLPSLLQFRIVAANDQGFSLSP
                     TYQINSFKNLGDLQIFVNGQAYVGPIEVLEDEQIQITWNDLSTANNAVNYKTEMSADL
                     IKWRI"
BASE COUNT          612 a          390 c          391 g          668 t
ORIGIN      
        1 gcggagtttg acccgattac ttggacgatt tcggaatgcc acaaacgggg aattgagttc
       61 catgcctggc ttaaccccta tcgtttaggt tccagcactt taggtttgtc tgttgaagat
      121 tatgcgaaaa ccttcccttc ctataatgtt gcttccaatc cggcttatat tttaaaagga
      181 acaacgggtg cgattttaaa tccgggtgaa atatctgtcc ggaagtttat cattgattct
      241 tgtatggaga ttattgagaa ctatgatgtt gatgccatcc attttgatga ctatttttat
      301 attggtggtg ttgatgatag tgcgacacga gctaaataca atttcaacaa tttaccgatt
      361 cctgattttc gtcggcaaca agtcgattta tttattgaag ctctttctag caaaatgcgc
      421 gaatataatc aattaaataa tcgtcatgtc caacttggca tttcaccaac cggtatttat
      481 cgtaatggtt cctatcaggc aacagcgact tatgatgcta agggctcatt aattactcct
      541 gttggttcca acacttcagg ttttgctcac tatggagatt atttatattg tgataccaaa
      601 aaatgggtgg ataatgaatg gattgattac attcttcccc aaagttattg gtcttttgag
      661 gaacccgcag ccgcttatgc ggatgtcatg gactggtggg tcaaagtagt taagaacaaa
      721 aaagttaacc tatattcggg aatggggatt tatcaagcgg ctgaagccaa gcgtgcttct
      781 tggaacacca acgttaatga agccggcaat caagttcaat atgccagtca atatccggaa
      841 ataaaaggcc attgtctttt tagttattcc caagtccaat cggcatattt aaccaaatcc
      901 gggatttttt ataataattt aaaccggatt cgccaattga tgtggacgaa taaagctgtt
      961 cttccggaaa tagaaacctt tacttccatt aaactttccc cagtaactaa tttagcaatc
     1021 agcaaaaatg accaaggttt tcgcttagat tttgataagc aaaccaaggc gaaaacttat
     1081 gtcatttatc gtagtcctag gcccattact tatgcaccta acgagattat tgatattatt
     1141 ggcaatgaat ttattgataa tcgcattagc tattttgata aaacgaattc ttcccaaaat
     1201 tattattacg ctcttaaagt gcaatccgca accaatactt taagtgaagg tgtggaaatt
     1261 aatacgaccc aagcaacttc gggagcttcg atgttaagac tggaaccgtt tgaaaatgtc
     1321 ttttttagtg attataatta ttttaatggg tttttaacgg tccgttggga tgaaatatac
     1381 cccttgtttg gtggtaaacc cagttatgaa gtgtattatt cacctgataa tattaacttt
     1441 cagcttttaa atacgacaac ttatccgctt gaggataacg gtggttacta tcaaacccgc
     1501 attcccctgg ataattcacc gacggtttat gtttatttaa aagctttcaa tgattatggc
     1561 tccgttgtca gtgaaactga agccctaacg attaccaaga ggattggtca tatttataat
     1621 ctcacagttc gcggagatgc ttttgcaggg gaaatgatta ccctaatgtg gaacaaattg
     1681 aaattaaccg atgttaatta tacggctcaa tattctattg atggctttaa ttggcataat
     1741 attacttcag ttgacaatcc gctcgttgaa gaaggacaaa attgttatca accctttatc
     1801 ctgcccaagc ttccttcact cctccaattt cgcattgttg ccgcaaatga tcaaggcttt
     1861 tccctttccc caacttatca aataaacagt tttaaaaatt taggtgattt acaaattttt
     1921 gttaacggtc aagcctatgt gggtcccatt gaagtgcttg aagatgagca aatccaaatc
     1981 acttggaatg acttatcgac cgctaataat gctgttaact ataagacgga aatgtcggct
     2041 gatcttatca aatggcgcat t
//
LOCUS       DAOSGN010000147         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_54309_length_2056_cov_2.587206, whole genome shotgun sequence.
ACCESSION   DAOSGN010000147 DAOSGN010000000
VERSION     DAOSGN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54309_length_2056_cov_2.587206"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1479
                     /locus_tag="PK087_02590"
     CDS             61..1479
                     /locus_tag="PK087_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOH18193.1"
                     /translation="MLTFYPDKQAKQPLYRQLYEFVKTMIQSGQLKTGEKMPSKRALS
                     AHLKLSQVTIDTAYQQLVMEGYLKAKAKSGFYVEPYDHPLIGSFLKTKALIKEKTLFD
                     YQWAFQTNVIDESFFPHALWGKLVRETLNSQKVGINQFDKQGLEELRLEIAKYLKNFR
                     GIICRPEQIVVGSGTESLLALLIPLLGTTLHYGMENPGYPKISQFFRNWDLLFSYVNL
                     DDHGLRVEELSKKQIDVVHVTPSHQFPMGIVMPLQRRLELLNWAKLKEKRFIIEDDYD
                     SEFRYLGQPIPALSSLDGLGKVIYMNSFSKSLAPSLRISYMVLPEALLQVFLERFSYH
                     SSSVPYFEQWVLAKFMEGGHFERHLNRMRKVYKNRLSFFQKVFGELGLDECFLLQGYD
                     AGLHFLMTTKKATEEALIKAAEKVKIKVLGLSEFTFQEANYPTSLVIGYSGLNEEQIK
                     TALTKLKTPWALCLTKPDNMIE"
     gene            1504..1944
                     /locus_tag="PK087_02595"
     CDS             1504..1944
                     /locus_tag="PK087_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOH18194.1"
                     /translation="MSLKETKQKDLQNLLIKYQIQPSLQRLQILAYLVSHYHPTAEEV
                     YSHLYDELGTLSKATVYNTLNLFVTAGLVKPLRIDERQMRYDMVTADHGHFQCDACGE
                     IYDFPLDETSIKEKPLVGFSVKQKFVYLKGTCSRCNKNSSGGSK"
     gene            1941..>2056
                     /locus_tag="PK087_02600"
     CDS             1941..>2056
                     /locus_tag="PK087_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015544254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOH18195.1"
                     /translation="MNENNRMPLIGDLAPAFQAVTTQGVINFPEDYQGKWVI"
BASE COUNT          639 a          365 c          437 g          615 t
ORIGIN      
        1 aatctgatat taataaaaga ctcagtttcg tctttatcga taatatcagt tggaggaata
       61 atgttaactt tttatccgga taaacaagca aaacaaccgc tttatcggca actttatgag
      121 tttgtaaaga cgatgatcca aagcggacag ttaaaaaccg gcgaaaaaat gccctcaaag
      181 cgggccttgt ccgctcattt aaaactcagt caggtaacga ttgatacggc ttatcaacaa
      241 ctggttatgg aagggtattt gaaagcgaaa gctaaaagcg gcttctatgt ggaaccctat
      301 gatcatccgc tgattggtag ttttcttaaa acaaaagctt taattaagga aaaaacctta
      361 tttgattatc aatgggcttt tcaaacaaat gtgattgatg aatcattctt tccccatgct
      421 ttatggggaa agctggtgag agaaactcta aattcgcaaa aggtcggcat taaccagttt
      481 gataaacagg ggcttgagga attaagactt gaaattgcca agtacttaaa aaattttcgc
      541 gggattatct gtcgtcccga acaaattgtt gtcggttcgg ggacggaatc tttactggct
      601 ttgttgattc ctttactggg caccaccctc cattatggga tggaaaatcc cggctacccg
      661 aaaattagcc aattttttag gaattgggat ttgttatttt cctatgtcaa tcttgatgat
      721 catggactaa gggttgaaga gttaagtaaa aaacaaattg atgttgttca tgtcacgcct
      781 tcccatcagt ttccgatggg catcgtgatg cccttgcaaa ggcgcttgga actgctcaac
      841 tgggccaaac taaaggagaa gcgtttcatt attgaagatg attatgatag tgaatttcgt
      901 tatcttggtc aacctattcc ggccctttca tccttggatg gcttgggaaa ggtcatttac
      961 atgaactcgt tttctaaaag tttagcccca tcactgcgca tcagttatat ggttttaccg
     1021 gaagctttgc tgcaagtgtt tttggaacgc ttttcttacc atagttcgag tgttccttat
     1081 tttgaacagt gggtgttggc aaaattcatg gagggtgggc attttgaacg ccacttaaat
     1141 cggatgcgca aagtgtataa aaaccgctta agcttctttc aaaaagtttt tggggaatta
     1201 gggcttgatg aatgcttttt gcttcagggt tatgatgccg gacttcattt tttgatgaca
     1261 actaaaaagg cgaccgaaga agcattaatc aaagcggcgg aaaaggtcaa gataaaagtt
     1321 ctcggtttaa gcgagtttac tttccaagaa gcaaattacc caacaagttt agttatcggt
     1381 tattccggat taaatgaaga acaaataaaa actgccctga caaaattaaa aacgccgtgg
     1441 gcattatgct tgacaaagcc tgataatatg atagaataat attagaaaga ttataaaatt
     1501 agaatgagtc taaaagaaac gaagcaaaaa gatttacaga atctgttaat taaatatcaa
     1561 attcaaccct cattacagcg tttgcaaata ttggcttact tagtcagtca ctatcacccg
     1621 acggcggaag aagtgtattc gcatttatat gatgaactgg gaactctctc caaagcgaca
     1681 gtttacaaca ccctcaattt gtttgtgact gcgggcttag taaaaccact taggattgat
     1741 gagcgccaga tgcgttatga tatggtaact gcggaccacg gccactttca atgtgatgct
     1801 tgcggggaaa tctatgattt cccgctggat gaaacatcga ttaaagaaaa gcccctcgtt
     1861 ggtttttctg ttaagcaaaa atttgtttat ttaaaaggga cttgttctcg ttgtaataaa
     1921 aatagttcag gaggtagtaa atgaacgaaa ataatcgtat gccgttaatc ggcgatcttg
     1981 ctcccgcttt tcaagcggta acaacccaag gagtaattaa ttttcccgaa gattatcaag
     2041 gaaaatgggt gatttt
//
LOCUS       DAOSGN010000148         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_54319_length_2056_cov_2.072464, whole genome shotgun sequence.
ACCESSION   DAOSGN010000148 DAOSGN010000000
VERSION     DAOSGN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54319_length_2056_cov_2.072464"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2056)
                     /locus_tag="PK087_02605"
     CDS             complement(<1..>2056)
                     /locus_tag="PK087_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18196.1"
                     /translation="VIPVGLEVVTKTNILGDQLEGVSEYVDINSLAEQLMEVDFLKEI
                     ENIGSIVADVSTLKIFESDLEGLALYMSFDTETVSNIFNSIGDLQLVDIIGNVGIAYL
                     LASEELATALDEMGLTTDDIDLSDVDWGDEIANLGNVYAAFKDFDITSTEFEQIDFNL
                     ITDEEINAFAEALFASKVLSNNSAVLVHVGLSLMPEEYRDFITVTNVEAKDLVSLLGL
                     GKVLIASGILNEDFDPVNLLTSGNIDALAEYISSSNLLSGSIGGIIEMLLSQVELPEG
                     LTITIDPNFDWSGESGKTELKALLKAAGKLVEMGIGEDDFIKNLSGDDINELADILAE
                     STILMSNIQNILEFFLSDETLTGGIKIELGDIDWTSAEGKTEFKALLGAVSAIFAAGI
                     LEDGFDFKTLEQATIDDLAAKLAASTVIRNNLTTIINQLVSGMDIDFTINAFEDPDDW
                     TELELKSLLSAVKIVLSKTNIPEDLFNLSEAELNTLLGSHLICDAIINVIKDFTAEGG
                     QLHGLLIIKNVSDDEWKDTLDDDGNVVVKGELRKLFTSAKILLGENINFDDPDGIIDP
                     NRILNLSDGTKDTNNDGVVDELDDDEIKEMLTSKILKDTIINKLIEFGKAAIDAASKM
                     SNAMVGLQSIVEGTGNSFEQATNFIDAYVSDGLVPATNAVTAYKNLLMRGYTTEQIEK
                     VMT"
BASE COUNT          642 a          457 c          321 g          636 t
ORIGIN      
        1 cagtcataac tttttcaatc tgttccgttg tatatccacg catcaataag tttttatacg
       61 ctgtaaccgc atttgttgca ggtactaaac catccgacac atacgcatct ataaaatttg
      121 ttgcttgttc aaaactgtta cctgttccct ctactattga ttgcagacca accattgcat
      181 ttgacatttt actagcagca tcaatagcag cctttccaaa ttcaattaat ttattaataa
      241 tggtatcttt taaaattttg gaagtaagca tttctttgat ttcatcgtca tccagttcat
      301 caacgacacc atcgttattg gtatctttgg taccgtcaga taaatttaga atgcgattgg
      361 gatcaataat gccatcggga tcatcaaagt tgatgttttc accaagaagg atttttgctg
      421 aagtaaagag ttttcgtaac tcccctttca caacaacatt accatcatca tccagagtgt
      481 ctttccattc atcatcactc acatttttaa taatcaataa accatgtagt tgaccacctt
      541 cggccgtaaa atctttgatt acattgataa tggcatcaca gattaaatgg gatcccaata
      601 aggtatttaa ttccgcttca cttaaattga ataagtcttc aggaatattc gttttcgata
      661 aaacaatctt aacggctgaa agcaaagatt ttaactctaa ttctgtccag tcatcgggat
      721 cctcaaaagc attgatggta aaatcaatgt ccattcccga taccaactga ttgataatcg
      781 tagttaaatt attgcgaata acggtggaag cagccagttt ggcggctaaa tcatcaatgg
      841 ttgcttgttc taaggtcttg aaatcaaatc catcttccaa gattcccgcc gcaaaaatcg
      901 cactgacagc gcctaataaa gctttaaatt cggtttttcc ctcggcactc gtccagtcaa
      961 tatcgcctaa ttcaatttta atgccaccgg ttaaagtttc atcacttaag aaaaattcca
     1021 agatattctg aatattgctc attaagatgg tcgattctgc aaggatatcc gccaattcgt
     1081 taatgtcatc accgcttaaa ttcttaataa agtcatcttc cccaatgccc atttccacaa
     1141 gttttccggc agcctttaat aatgccttta gctcggtctt accggattca ccgctccagt
     1201 caaagtttgg atcgatggta atcgttaaac cttccggcaa ttccacttgg cttaataaca
     1261 tttcaataat gccaccgata ctgccactaa ggagattgga actggagata tattcggcca
     1321 aagcatcaat atttcctgaa gttagtagat taaccggatc aaaatcttcg tttaaaatac
     1381 cgctcgcaat taaaactttt cctaaaccaa ggagagaaac taaatccttg gcctccacat
     1441 tggtaacggt aataaaatca cgatattctt ccggcattaa acttaaaccg acatgaacta
     1501 agactgcact gttattggat aaaaccttgg aagcaaacag cgcttcagca aaagcattga
     1561 tttcttcatc cgtaatgaga ttaaaatcaa tttgttcaaa ttcggtcgag gtgatatcaa
     1621 agtccttgaa agccgcataa acattgccta aattggcaat ttcatcgccc caatcaacat
     1681 cgcttaaatc aatgtcatcg gtagttaatc ccatttcatc taaggcggtc gccagttctt
     1741 cactcgctaa caaataagca atcccgacat taccgataat atcgaccaat tgcaagtccc
     1801 cgatggaatt aaagatgttt gatactgttt cggtatcaaa gctcatgtaa agggctagtc
     1861 cttctaaatc cgattcaaaa atcttaagcg tactgacatc ggcaacaatc gacccgatgt
     1921 tttcgatttc tttcaagaaa tcaacttcca taagttgttc agctaaactg ttgatatcaa
     1981 catattcgct aacgccctct agttgatcac ctaaaatatt ggtttttgta acaacttcca
     2041 aaccaacggg aataac
//
LOCUS       DAOSGN010000149         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_54385_length_2054_cov_2.791396, whole genome shotgun sequence.
ACCESSION   DAOSGN010000149 DAOSGN010000000
VERSION     DAOSGN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54385_length_2054_cov_2.791396"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="PK087_02610"
     CDS             <1..1409
                     /locus_tag="PK087_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18197.1"
                     /translation="NDLQTIRATFGQGTVMLIDAIFLGVLAFVKMWRMDIVLTLISSI
                     PLLLLALSSRIIGKYMSKKFALRQKAFANMADFTQESISGISVVKAFVKEAKELMAFT
                     KINKENYDRNMEFVKAGVLLHTLIGGLIASIIVIIMAYGGYLVYQSQVNQNLMFTVGD
                     LTKYISYFGTLTWPMMAIAQLINLRSQAQASLQRIDGLLNEEIDIKDSYPYQIEAITG
                     AITFNNLSFKYPQTNKLVLENISLTIEAGEKIGIIGRTGCGKTTLVDLLLRLYNLEPN
                     RILLDGIDIMRLPITKVRDAIAYVPQDNFLFSDTIENNINFAFQEKDVEKIQQAAKMA
                     DLDGNVREFKDGYQTVLGERGVTISGGQKQRTAIARALMKDAPILTLDDSVSAVDTKT
                     EEIILNNIHKIRQGKTTILIAHRISTIQSMDKIILLDEGKLVGFGSHAELLQTSVLYK
                     QMVDLQKLEDEVGGEIDG"
     gene            1402..>2054
                     /locus_tag="PK087_02615"
     CDS             1402..>2054
                     /locus_tag="PK087_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18198.1"
                     /translation="MDEEVTKVNLTDKKIIKRLISYAKNHKKAFAIAVLLMVFAVGID
                     LVLPLLLGLVSKILGRDNIDFQQIIGIVVIYGCLLVVMFIIQFYQAMTLQKAGQKIIY
                     EVREQVFTHIEKYSIAQINSVPVGKFVTRVTNDTNTLNEMYTSVVINLLKDSFTIIGV
                     LVAMFFVDTSLSLYVLAVCPLIVIISFVFRKYSREAYREVRSNIANVNAFLSENISG"
BASE COUNT          601 a          380 c          422 g          651 t
ORIGIN      
        1 cgaatgattt gcaaaccatt cgggccactt tcggtcaagg gacggtgatg ttaatcgatg
       61 ctatattttt gggtgtttta gcctttgtaa agatgtggcg catggatatc gtcctaacct
      121 taatatcatc gattcccttg cttcttttag ctttatccag tcggattatt ggcaagtata
      181 tgagcaagaa atttgcctta cgccaaaaag cctttgcgaa catggcggac tttacccaag
      241 aatcgatttc cgggatttcc gttgtcaagg cgtttgtgaa agaagccaaa gaattaatgg
      301 cctttacaaa gattaacaaa gaaaattacg accgcaatat ggagtttgtg aaagcgggcg
      361 ttttattgca tacattaatt ggcggcttga ttgcttccat tattgtgatt attatggctt
      421 atggtggtta tttagtatat caatcgcaag tcaatcaaaa tctcatgttt acggttggcg
      481 atttaacgaa atatatttct tattttggca ccttaacttg gccgatgatg gccattgctc
      541 aactcatcaa tttacgaagt caagcccaag cctccttgca acgcattgat ggcttgttaa
      601 acgaagaaat tgacattaag gatagctacc cctaccaaat agaagcaatt accggcgcca
      661 tcacctttaa taatttatca tttaaatatc cccaaacaaa taaactcgtt ttggaaaata
      721 tttccttaac cattgaagcc ggtgaaaaaa tcgggattat cggccgcacg ggttgtggga
      781 aaaccacttt ggtggattta ctgttgcgct tatataatct tgaacctaac aggattctct
      841 tagatggtat tgacattatg cgcttaccga ttactaaagt tcgggatgcc attgcctatg
      901 tcccgcaaga taacttctta ttttccgata cgattgaaaa taatatcaac tttgctttcc
      961 aagaaaaaga tgttgaaaaa atccaacagg cggccaagat ggctgatttg gatggtaatg
     1021 ttcgtgaatt taaagatggc tatcaaaccg ttttaggaga acggggtgtt actatttccg
     1081 gtggccaaaa acaacggacg gcgattgcca gagccctaat gaaagatgcc ccgattctta
     1141 ctttagatga ttcggtttcc gctgtggata cgaaaaccga agaaatcatc ttaaacaata
     1201 ttcataaaat tcgccaaggg aaaacaacga ttttaattgc ccaccgcatt tcgaccatcc
     1261 aatcgatgga taaaattatt ttactcgatg agggcaagct tgttggtttt ggctcccatg
     1321 ctgaactctt gcaaacaagt gttctttata aacaaatggt tgatttacaa aaactggagg
     1381 atgaagttgg aggtgagatt gatggatgaa gaagtaacaa aagtcaattt gaccgataaa
     1441 aaaatcatca agcgcttgat atcttatgcg aaaaaccata aaaaagcttt cgccattgcc
     1501 gttcttttaa tggtttttgc cgttggtatc gatttggttc tccccttatt gcttggctta
     1561 gtgtcgaaaa tcttaggtcg tgataatatc gattttcaac agattattgg gattgttgtt
     1621 atctatggtt gcttgttagt ggtgatgttc attattcagt tctaccaagc gatgacttta
     1681 caaaaagccg gccaaaagat tatttatgaa gtgcgtgaac aagtctttac ccatattgaa
     1741 aaatactcga ttgcacaaat taacagtgtt cccgttggca aatttgtcac ccgcgtcact
     1801 aacgatacga ataccttaaa tgaaatgtat acgagtgtcg ttatcaattt actgaaagat
     1861 tcgttcacca ttatcggggt tttggtggcg atgttctttg tggacacaag tttaagttta
     1921 tatgtgttgg ccgtctgccc tttgattgtc attatttcgt ttgttttccg aaaatattcc
     1981 cgggaagctt accgcgaagt tcgcagtaat atcgccaatg tcaatgcgtt tttgtcggaa
     2041 aatatttcag gaat
//
LOCUS       DAOSGN010000150         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_54593_length_2049_cov_2.829990, whole genome shotgun sequence.
ACCESSION   DAOSGN010000150 DAOSGN010000000
VERSION     DAOSGN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54593_length_2049_cov_2.829990"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2049)
                     /gene="mutS"
                     /locus_tag="PK087_02620"
     CDS             complement(<1..>2049)
                     /gene="mutS"
                     /locus_tag="PK087_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOH18199.1"
                     /translation="LNEDLLITEILKLKTREIVIGNDFPTHLLEQINTFHPLTISKED
                     NTSPINYLKALTEELDKGEEKAFLRLLNYIVRTQKRHLIHMQKVVKYDASGFMKIDFA
                     SRRNLEILETLRFQNKKNSLVNVLDRCVTAMGSRFLKKSLIFPLIDREKIEKRYGIVE
                     KCQQEFLLANELRSHLESVYDLERIVGKISYDSANPKDLLQLHKSLGVVPNIQALIQK
                     LKIQHYFNLSAHLDVFFDLYHLLDQSIAIDAPFVVKDGGVIKDGYHEELDSFRRLVSE
                     SKDYLLTLEEKERERSGIKNLRVGFNRVFGYYLEVSKGNSAMVQEKLGYIRKQTLSNV
                     ERFITEELKDKESMILRAEEKALELEIKIFSEIRERAKLTIANLQYLAKVLAELDMLL
                     SFARVANENHYVRPKLMEQMVIDLKASRHPVIEKFQDHFIPNDVVMKEDDYILLITGP
                     NMSGKSTYMRQVALIAIMAQIGCFVPCASARLPIFDAIFTRIGAADDIVSGQSTFMIE
                     MQEVNNALKHATKQSLVIFDEIGRGTATYDGMALAQAIIEFIHEQKLCKTLFSTHYHE
                     LTTLDKDLSHLRNVHVSAEEVHGDIVFLHKVLPGPVDKSYGINVAKLANIPLEVILRA
                     SDILKKLENHHKIDSKALSIKNYQPPMLYDSKSEKEVFVLEQIKSIDINQLSPLEAMN
                     "
BASE COUNT          661 a          408 c          325 g          655 t
ORIGIN      
        1 tattcatcgc ttcaagagga cttaactgat tgatatcaat acttttaatt tgttcaagaa
       61 caaacacttc tttttctgac tttgaatcat agagcattgg cggctgataa ttcttgattg
      121 acagcgcttt actatcaatt ttatgatgat tttctaattt tttaagaatg tcactggctc
      181 ttaaaattac ttcaagagga atgttagcca gtttcgcaac attaatgcca taactcttat
      241 caaccggccc cggtaaaacc ttatgtaaaa agacgatgtc accatggact tcttcagccg
      301 atacatggac attgcgtaaa tggcttaagt ccttatccaa agttgtcaat tcatgataat
      361 gagtggaaaa taaagtttta cacaattttt gttcatgtat aaattcaata atcgcttgtg
      421 ctaaagccat gccgtcatag gtggccgtac cacgaccaat ctcatcaaaa ataactaaac
      481 tctgcttagt tgcatgctta agagcattat tgacttcttg catttcaatc ataaaggttg
      541 attgaccgct aacaatatca tcagccgctc caattcttgt aaaaatggca tcaaaaattg
      601 gtaaacgggc gctggcacag ggaacaaaac atccgatttg tgccataatt gcaattaagg
      661 ccacttggcg catataggtt gattttccgc tcatattcgg accggtaatt agtaagatat
      721 aatcatcttc tttcataaca acatcattgg gaataaaatg atcctgaaat ttctcaatga
      781 cgggatgtcg cgaagctttc aaatcaatca ccatctgctc cattaatttt gggcgcacat
      841 agtggttttc attagcaacc cgagcaaaag aaagtaacat atccagttcg gccaagactt
      901 tcgctaaata ttgtaaatta gcaattgtta gttttgctcg ttcgcgaatc tcactaaaga
      961 tcttaatctc taattccaaa gctttttctt ctgctctaag aatcatactt tctttatctt
     1021 tcagttcctc ggtaataaaa cgttcaacat tgcttaaggt ttgcttgcga atatagccga
     1081 gtttctcttg aaccatggcg ctgttgcctt tagaaacttc taaataatac ccaaaaacgc
     1141 gattaaaacc aacgcggaga ttcttaatcc ccgaacgctc ccgttctttt tcctccaagg
     1201 tcaataggta atccttgctt tcactaacta agcggcgaaa actatccaac tcttcatgat
     1261 agccatcttt aataaccccg ccatccttaa caacaaacgg tgcatcaatg gcaatacttt
     1321 gatcaagtaa atgatataaa tcgaaaaaaa catctaaatg cgcacttaaa ttaaaataat
     1381 gttggatttt cagtttttga attaaggctt gaatattcgg aacaacaccc aaggacttat
     1441 gcagttgtaa caaatctttc ggattagcac tatcataaga tattttccca acaattcgtt
     1501 ctaaatcata aacgctttcc aaatgagaac gaagttcatt agcaagaaga aattcttgtt
     1561 ggcatttttc gacaatccca taacgttttt cgattttttc acgatcaatc aaggggaaaa
     1621 taagggattt ttttaaaaaa cgacttccca tcgccgtcac acaacgatct aaaacattaa
     1681 ccaaagagtt cttcttgttt tgaaaacgca aggtttctaa aatttccaaa ttacgacgag
     1741 aagcaaaatc aattttcata aagccactgg catcgtactt caccactttt tgcatatgaa
     1801 ttaaatggcg tttttgggta cgaacaatat aatttaacag ccttaaaaaa gctttttctt
     1861 cgcctttatc caattcttcc gttaaagctt ttaaataatt aataggagaa gtattatctt
     1921 ccttggaaat ggtaagcgga tggaaagtat tgatttgttc aagcaaatga gtgggaaaat
     1981 cattgccaat gacaatttcc cgcgttttta attttaaaat ctcggttatt aaaagatctt
     2041 catttaagg
//
LOCUS       DAOSGN010000151         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_54924_length_2041_cov_2.148540, whole genome shotgun sequence.
ACCESSION   DAOSGN010000151 DAOSGN010000000
VERSION     DAOSGN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54924_length_2041_cov_2.148540"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1102
                     /gene="dusB"
                     /locus_tag="PK087_02625"
     CDS             143..1102
                     /gene="dusB"
                     /locus_tag="PK087_02625"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013399821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOH18200.1"
                     /translation="MENLAIKGKLALAPLAGYTNLAYRSIMKEWGADLVFSEMVSAKG
                     LVYESAKTWELTAIDEAEHPLAIQIFGGDLEAMVKAAMMIDQQTTADIIDINMGCPVR
                     KVLKAEGGCFLLSDPKRIETLVREIVKHVQKPVSVKMRAGISHQTINAVANAQAIERA
                     GASFITIHGRTQSDMYAGKVRLDYIKAVKEAVTIPVIGNGDIKSVEDAHTMLEETKVD
                     MIMVGRGSLGNPWLIRDLAHYLKGLPIPPPPTPFEKVKMCKHHFDRLLSIKPLHQALL
                     EMRSLAAWYVKGINHSKDFRKQLHEITGKEQLFNLLDNLLAQQ"
     gene            1099..>2041
                     /locus_tag="PK087_02630"
     CDS             1099..>2041
                     /locus_tag="PK087_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOH18201.1"
                     /translation="MKTYAMREVNNELYLGNIRLSELVKDHPTPLYVYDEAGIEHKIS
                     QFRTHFTSKQFFCETIYASKAFLAPYLCQMLEKAGFGIDAVSLGDLYLIKKSGFPLAK
                     VVFHGNNKSDEELQTAIKERVGLIVVDNLNELKRLMTFAEAVDYPIKTLFRVNPGISA
                     HTHAYIETALLSSKFGESIYDWDKMHSLAKLYQESRNVKLLGFHAHIGSQINTPSSFV
                     KSAQTMLNFMAKFQEETKMVLNTLNLGGGFGIKYLPKDKEIKLPEMLTKIVKTVEKGV
                     KEKGLALRTLMIEPGRSLVGDSGFTLYRCGGTKQTFGH"
BASE COUNT          625 a          373 c          461 g          582 t
ORIGIN      
        1 attttaccat aatcaatgaa agtgtcaacg ctatttattt tcagcacctt tcttttttcc
       61 ttcttttcgc aaaattcttt aaaaaacgct ctttcttatg gtataatatc acagagtgta
      121 aaatgagaaa aaggataaaa aaatggagaa cttagcgatt aagggaaaac tcgctttagc
      181 gcctttagcc ggctatacca accttgctta tcgcagtatt atgaaagaat ggggcgcgga
      241 tttggtcttt tcggaaatgg tcagtgccaa aggtttagtc tatgaaagcg ccaaaacctg
      301 ggaattaacg gctattgatg aagcggaaca cccgcttgct atccagattt ttggtggtga
      361 tttagaggcg atggttaaag cggcgatgat gattgaccaa caaacgacgg ccgacatcat
      421 cgatatcaac atgggttgtc cggtgcgcaa ggtcttaaaa gccgagggcg gatgcttttt
      481 attgtccgac cctaaacgga tagaaacttt agtgcgagaa attgtcaagc atgttcaaaa
      541 accggttagt gtcaaaatgc gggcggggat aagccaccaa acgattaatg ccgttgccaa
      601 tgcgcaagcc attgaacgag cgggagcgtc atttattacc attcatggtc ggacgcagtc
      661 cgatatgtat gccggaaaag ttaggcttga ttatattaaa gctgttaaag aggcagtcac
      721 gattcctgtt atcgggaatg gtgatattaa gtccgtggaa gatgcgcaca cgatgcttga
      781 agaaacaaaa gtggatatga ttatggtcgg gcgcggcagt ttaggaaatc cgtggttaat
      841 tcgcgatttg gcacattatt taaaaggttt accaatcccg ccaccaccaa caccgtttga
      901 aaaagttaag atgtgtaaac atcatttcga ccgcttgtta agcattaaac cgcttcacca
      961 agcccttttg gaaatgcgca gtttagccgc atggtatgtg aagggcatta atcacagtaa
     1021 agattttcga aagcaacttc atgaaatcac aggaaaggag caattattta atttattaga
     1081 taatttattg gcccagcaat gaaaacctat gcaatgagag aagtaaacaa tgaattatat
     1141 cttggcaaca tccgtttaag cgaacttgtg aaggatcatc cgacgccctt atatgtctat
     1201 gatgaagcgg gaattgagca taaaattagt cagtttcgca ctcattttac aagtaaacaa
     1261 tttttttgtg aaacgattta tgcttccaaa gcttttttag caccctattt atgccaaatg
     1321 cttgaaaaag ccggcttcgg cattgatgcc gtgtccttgg gggatttata tttgattaaa
     1381 aaaagtggtt ttccgctggc caaagtcgtt tttcatggca ataataaaag cgatgaggaa
     1441 ttgcaaacgg ctattaagga aagagttggt ttaatcgttg tggataatct taatgaatta
     1501 aagcgcttga tgacttttgc ggaagcagta gattacccaa tcaaaacctt gtttcgggtt
     1561 aatcccggga tttccgccca tacccatgct tatattgaaa cagctttgtt gtcgtctaag
     1621 ttcggggaat cgatttacga ttgggataaa atgcatagct tagcaaaatt atatcaagag
     1681 tctaggaatg tcaaactcct aggctttcat gctcatatcg gttcgcagat taatacgccc
     1741 tcttcttttg tgaaaagtgc gcaaacgatg cttaatttta tggcgaagtt tcaagaagag
     1801 acgaagatgg tgctaaacac tcttaattta ggtgggggct tcgggattaa gtatttacca
     1861 aaagacaaag aaatcaagct tcccgaaatg cttacaaaaa ttgttaaaac cgttgaaaaa
     1921 ggtgttaagg aaaagggact ggctttaaga actttaatga ttgaaccggg acgcagtttg
     1981 gtcggtgatt ccggttttac cctctaccgt tgtggcggca ccaaacaaac cttcgggcat
     2041 a
//
LOCUS       DAOSGN010000152         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_55162_length_2035_cov_2.863636, whole genome shotgun sequence.
ACCESSION   DAOSGN010000152 DAOSGN010000000
VERSION     DAOSGN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55162_length_2035_cov_2.863636"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK087_02635"
     CDS             <1..543
                     /locus_tag="PK087_02635"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOH18202.1"
                     /translation="PADFTPVCTTEFMTFATMADEFRALNTELVGLSVDSLYAHIAWL
                     RKIQELEWNGMKNVEVKFPLIEDIKMDVANKYGMIQGQSTTQAVRAVFFIDPKGIVRT
                     ILYYPAAVGRNFDEIKRIILALQKADRDSVATPANWRPGQDTIIPTAGSCGVAKQRME
                     SQDENKYCLDWFLCFNKEKK"
     gene            751..1656
                     /locus_tag="PK087_02640"
     CDS             751..1656
                     /locus_tag="PK087_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOH18203.1"
                     /translation="MEKQEFKIALMIDSENVSPKYVKSVINEIAKYGKIIIARFYGDV
                     SKLSAEWHQMALDYAIKPVHQYNVATGKNAADLAMALDAQEMMYQQKVNSFFLVTSDS
                     DFTPLAMKLKEGGMHVIGVGSEKKVSSAFKNACNEFKYFEYLDESHEEEPTETTCAGN
                     ADIASIIKGVIVESGVDNKIPLSVLGGILVNRFSDFDPRKYGAKSLSALVNTICDLSI
                     VQEKQTPYVVLKTKLSLKDIGEVIIQIINRNKKKEMMLTKIKEELEKMNPDFDFQELG
                     FTRFSKLVASVKGVFVKDNKAILRN"
     gene            1834..>2035
                     /locus_tag="PK087_02645"
     CDS             1834..>2035
                     /locus_tag="PK087_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HOH18204.1"
                     /translation="MKKIWLVVLLISLGFLLGSCKHQKEHLTFEMSELGQAVMYEVNI
                     RCYTEAGTFLAFKEHLPRLKELG"
BASE COUNT          639 a          317 c          445 g          634 t
ORIGIN      
        1 ccggctgatt ttactccggt atgtaccacc gaatttatga catttgccac catggcggat
       61 gaatttaggg cccttaatac cgaattagtc ggtttaagtg ttgattcgct ctatgcccat
      121 attgcttggt taagaaaaat ccaagaattg gaatggaacg gaatgaagaa tgtggaagtt
      181 aaattcccct taattgaaga tattaaaatg gatgttgcca acaagtatgg aatgattcaa
      241 ggccaatcga caacgcaggc ggtgcgggcg gttttcttca ttgatcctaa aggcattgtc
      301 agaaccatcc tttattatcc ggctgctgtt ggtcgcaatt ttgatgaaat caaacggatt
      361 attttagctt tgcagaaagc tgaccgtgac tcggtcgcca ccccggctaa ctggcgtcct
      421 ggtcaagaca cgattattcc gacggcaggt tcgtgcggag tggctaaaca gcgtatggaa
      481 tcccaagatg aaaacaaata ttgtcttgat tggttcttat gtttcaacaa agaaaagaag
      541 taaactaaaa acttctttaa atattccttc tttatttttc aaaaggctga gtcttattgc
      601 gactcagtct tttttttgtt tataaaaaaa ggaaactaaa ggtgtaaacc ttcgatttat
      661 gttaatgtga ctgataaatt gcttggatgg aaagaaaaaa tgcatgttaa tttttggatt
      721 attatggtat aatgacttta gaggttaaac atggaaaaac aagaatttaa aattgccctt
      781 atgattgatt cggagaatgt ttcccccaaa tatgttaaat ccgtcatcaa tgagattgcg
      841 aaatacggca aaatcatcat tgcccgtttt tatggggatg tttcaaaact atcagcggaa
      901 tggcatcaaa tggctttgga ctatgccatt aagcccgttc atcaatacaa tgtcgctacc
      961 ggcaaaaatg ctgctgattt agcgatggct cttgatgctc aggaaatgat gtatcaacaa
     1021 aaagtcaatt ccttcttttt agttacttct gatagcgact ttacaccgct tgccatgaag
     1081 ttaaaagaag ggggaatgca tgtgattggc gttggtagtg agaaaaaagt ttcttcggct
     1141 tttaagaatg cttgcaatga atttaaatac tttgaatatt tagatgaaag ccacgaagaa
     1201 gaaccgacgg aaaccacttg tgccggcaat gccgatattg cttcaattat taaaggcgtg
     1261 attgtggaaa gcggtgtaga taataaaatc cccctaagtg tcttaggcgg cattttagtc
     1321 aaccggtttt cagactttga tcctcggaag tatggagcga agagtttatc ggctttagtc
     1381 aatacgattt gtgatttatc gattgttcag gagaaacaaa ctccctatgt ggttttaaag
     1441 actaaattgt cgttaaaaga tattggagaa gtcattattc agattattaa tagaaacaag
     1501 aaaaaggaaa tgatgttaac gaaaatcaaa gaagaactgg aaaagatgaa tccggatttt
     1561 gattttcaag aactgggctt tacccgtttt agtaaattag tggcttccgt taaaggtgtc
     1621 tttgtgaagg acaacaaagc cattttaagg aattgataat ataaaaccga aaggactctc
     1681 tttcggtttt ttgaaatttc gcttgctttg cttttttgtt taatcttgtt tattaccggt
     1741 tgagttggga aaaccccagt agggaagcaa tattaggtga attatcgcag ataaaaagtt
     1801 ttggtagata ggaatcctat tggaaggtga aaaatgaaga aaatttggct agtggtatta
     1861 ttaataagtt tagggttttt gttaggttct tgtaagcatc aaaaagaaca tttgacattt
     1921 gaaatgtcgg agttaggaca agcagtgatg tatgaagtca atattcgctg ttatactgaa
     1981 gctggaactt ttttggcttt caaggaacat ttaccaagac tcaaggaact tgggg
//
LOCUS       DAOSGN010000153         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_55421_length_2029_cov_2.118034, whole genome shotgun sequence.
ACCESSION   DAOSGN010000153 DAOSGN010000000
VERSION     DAOSGN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55421_length_2029_cov_2.118034"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..765
                     /locus_tag="PK087_02650"
     CDS             13..765
                     /locus_tag="PK087_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH18205.1"
                     /translation="MNEIIYKKTTGLTDTPLSYCPGCTHGIIHKLVAEVMEELGLLNK
                     AIGVASVGCSVMSYYFFNCDMQQAAHGRACAVATGIKRVHPDNIVFTYQGDGDLASIG
                     IAETIHAANRGENITVIFVNNAIYGMTGGQMAPTTLIGQKTTTSPFGRNEADHGMPIR
                     ISEMLATIYKAAYIERVALTTPRAVKKAKESVRKAFTYQMEKKGFTLIEFLSTCPVNW
                     GMTPLQAMKWVDENMAKYFPLGVFKDVGEQHE"
     gene            758..1312
                     /locus_tag="PK087_02655"
     CDS             758..1312
                     /locus_tag="PK087_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH18206.1"
                     /translation="MNKAKRITIAGFGGQGVMMIGQMLAYAGNEADLNSLWFPSYGPE
                     TRGGTANCSVTLSPTLINSPVFSKADTLIIMNKPSLTKFWDKIIVNGTLLYNSSLITE
                     PVVRNDCQVYGIPVVDLALELGNVKVANMIILGAYLYLNPDFNDEIIKKVLEKILGED
                     KQHLVDINIKAIQLGKDYIAKLVK"
     gene            1314..>2029
                     /locus_tag="PK087_02660"
     CDS             1314..>2029
                     /locus_tag="PK087_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOH18207.1"
                     /translation="MLSLKDLLLAAQNLACQKIAVVSANDEAVVEAVEQARQMGFIRP
                     VLIGPKEQISPIILTKKYHLPHYEIIDACSDEQASLIAMDLVNRHQIDFIMKGLLDTK
                     ILLKAVVNKERGIRKAPLLSHVGIVSYPDFNRLLFVTDGAMNIAPDVNEKMAIIANAV
                     ELAKTLGYEQPKVGLVAAVEKLNPKMASTVDAEALVNLYQQGLIKDCLLDGPFAIDNL
                     VSKEAALHKGIKSEVAGQAD"
BASE COUNT          607 a          365 c          441 g          616 t
ORIGIN      
        1 gaggcaagaa ccatgaatga aatcatctat aagaaaacaa cgggcttaac ggatactcct
       61 ctttcttatt gtccgggttg tacccatgga atcattcaca aattggtcgc ggaagtcatg
      121 gaagaattgg ggcttttgaa taaagcgatt ggtgttgcca gtgtcggctg ttccgtcatg
      181 tcttattact tctttaattg tgatatgcaa caagctgccc atggccgggc ttgtgcggta
      241 gcgacgggaa ttaagcgtgt tcatcccgat aatattgttt ttacttatca aggtgatggc
      301 gatttagcat caatcgggat tgcggaaacc atccatgcag ctaatcgagg cgaaaatatc
      361 accgttattt ttgttaataa tgccatctat ggtatgacgg gtgggcaaat ggcccctaca
      421 accctaattg gtcagaagac gacaacttca ccttttggtc gtaatgaagc tgatcatgga
      481 atgccaatta gaatttccga aatgttagca accatctata aagccgctta cattgaaaga
      541 gtcgccttaa ccacgccacg agcggtgaaa aaagctaaag aatctgttcg caaagccttt
      601 acttaccaaa tggaaaagaa gggctttacc ttgattgagt ttttgtcaac ttgtcctgtt
      661 aactggggca tgacacccct tcaagcgatg aaatgggttg atgaaaatat ggctaaatat
      721 tttcctttag gagtgtttaa ggatgtcggt gaacaacatg aataaagcta aacgaattac
      781 gattgctggt tttggcggcc aaggcgttat gatgattggc caaatgttgg cttatgcggg
      841 caatgaagcc gatttaaata gtttatggtt tccttcctat ggaccggaaa ccagaggcgg
      901 cacggccaat tgttccgtta ctttatcacc aactttaatc aattcgccgg ttttttcgaa
      961 agcggatacc ttaatcatta tgaataaacc atccttaact aagttttggg ataaaattat
     1021 tgttaacgga accttacttt ataattcatc cttgattacc gaacccgttg tgcgtaatga
     1081 ttgccaagtt tatggtattc cggtggtgga tttagcgctt gaacttggga atgttaaagt
     1141 tgccaatatg attattttag gagcttattt atatttaaat ccagatttca atgatgaaat
     1201 tattaaaaaa gttttagaga aaattcttgg tgaagacaaa caacacctag ttgatatcaa
     1261 tattaaagct attcaattag gaaaagatta tatcgctaaa ttggtgaaat aaattgctaa
     1321 gtttaaaaga tttattgctg gcggcccaga atttagcttg tcagaaaatc gccgttgtaa
     1381 gtgctaacga tgaagccgtt gttgaggctg ttgaacaagc caggcaaatg ggctttattc
     1441 gtccggtttt aattggtccg aaagagcaaa tctccccaat aatccttact aagaaatatc
     1501 atttaccaca ttatgaaatc attgatgctt gttccgacga acaagcatca ttaatagcca
     1561 tggatttagt caatcgccat caaattgatt tcattatgaa aggtttactt gatacgaaga
     1621 ttttattaaa agcggttgtc aataaggaaa gggggattcg caaagctccc ttattatccc
     1681 atgttgggat tgtttcttat ccagacttta atcgtttatt atttgtaacg gacggagcca
     1741 tgaatattgc tcccgatgtc aatgaaaaaa tggccatcat cgccaatgct gttgagcttg
     1801 cgaaaacctt gggttatgaa caacccaaag tcggtttagt ggccgctgtg gaaaagctta
     1861 acccgaaaat ggcttccacc gttgatgccg aggctttagt taatttatat caacaaggtt
     1921 taataaaaga ttgcttgctt gatggaccct ttgcgattga taatttggtt tctaaggaag
     1981 cagctttaca taagggcatt aaaagtgaag tggcggggca agccgacat
//
LOCUS       DAOSGN010000154         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_55619_length_2024_cov_3.040122, whole genome shotgun sequence.
ACCESSION   DAOSGN010000154 DAOSGN010000000
VERSION     DAOSGN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55619_length_2024_cov_3.040122"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..864
                     /gene="udk"
                     /locus_tag="PK087_02665"
     CDS             232..864
                     /gene="udk"
                     /locus_tag="PK087_02665"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOH18208.1"
                     /translation="MKTTIIGIAGGTASGKTTVAKKLFEQSHALGSVVVIRLDDYYHE
                     NSHLTLAERKNINYDHPSAYDSPLLIKHLKMLKQGKSIDKPTYDFVNHNRALLVEQIA
                     PADVIIVEGIMIFAIPELRKMFDIKLFVHTPDDVRFIRRLMRDINERGRSIDSVINQY
                     LTTVRPMHLEFVEPSKVHADLIIPEGGHNDVAIDIIVSKIRNIMKEKRPN"
     gene            complement(878..>2024)
                     /gene="nagA"
                     /locus_tag="PK087_02670"
     CDS             complement(878..>2024)
                     /gene="nagA"
                     /locus_tag="PK087_02670"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOH18209.1"
                     /translation="TISGFKDSVIYVAGLGLYKTSLSINQGKISSLNSAPADAMSLPE
                     DLIVVPGFINRHAHGANHSDAMYPSYKDLHNIALTLASEGITTFLATTMTQTPDNIDQ
                     ALKNIREYIKKHPDAGALVYGVHLEGPFISKKHKGAQPEHAIVPADVDSFKHYQTTSG
                     NNIKQVTLAYEENGEALVKYLVSQGIVASLGHSDATAKDLQAAADVGLTSLTHTFNAM
                     RPLHHREVGALGAALLDERIYLEVIADLIHVSKEALQVLYRLKGPKKVTLVTDSIEAS
                     HMPDGIYQLGGQEVIVKGNEARLSSGALAGSTLLMNDAIRNFKEVNNLSLTEAIDLAT
                     INPARCLGIDSIKGEIALGKDADFTVIDQDLQVYMTVRNGKMIYKKS"
BASE COUNT          615 a          442 c          383 g          584 t
ORIGIN      
        1 aaattcaaag attttttctt gataattggt ggtggcggta acttggggct gaaagaaaaa
       61 aatactcatt aagcccagca aaaacgccat taagaataat ttacattgtc tcacaatttc
      121 acctcttgaa cgattatacc atagttgctc cttaaaaacc agtaaaaacc gctttttaaa
      181 ttgactaata agcccctttt ttgtataatc aaaccaacta agggaggagc tatgaagaca
      241 accattattg gcattgctgg gggaaccgcc agcggaaaaa ccacggttgc gaaaaaactg
      301 ttcgaacaat cccatgcttt aggatcagtc gttgttatta ggttggatga ttattaccat
      361 gagaactcgc atttaacatt agccgagcga aaaaacatca attacgatca tccctctgct
      421 tatgatagcc cattattaat caaacattta aaaatgctga aacaaggaaa aagcatcgat
      481 aaacccactt atgattttgt taaccataat cgggccttgt tggtcgaaca aatcgctccc
      541 gccgatgtca ttattgtgga aggaattatg atttttgcca tccccgaact acgaaaaatg
      601 tttgacatca aattgtttgt gcacaccccg gatgatgtcc gctttatccg ccggttgatg
      661 cgtgacatta acgaacgagg ccggagcatt gactcagtca tcaatcagta tttaacgacc
      721 gtccgcccaa tgcatttaga gtttgtggaa ccatcgaaag tccatgccga tttaatcatt
      781 cctgaaggag gacataatga tgtcgccatt gacatcatcg tcagtaaaat ccgtaatatt
      841 atgaaagaaa aaaggccgaa ctaatcggcc caccaaatta ggactttttg tatatcatct
      901 tcccatttct cactgtcata taaacctgta aatcttgatc aataacggta aagtcagcat
      961 cttttcctaa agcaatttct cctttaatac tgtcaattcc caaacaacga gccggattaa
     1021 tcgtcgctaa gtcaatggct tccgttaagg acaaattgtt aacttcttta aaattacgga
     1081 tggcatcatt catcaacaaa gtgcttcctg ctaaggcacc cgatgataag cgagcctcat
     1141 ttcccttaac aatgacttcc tgcccgccca attggtaaat cccgtcgggc atatggctgg
     1201 cttcaatgct gtcggtaact aaagtaactt tcttcggtcc ttttaagcga tataaaacct
     1261 gaagggcttc tttcgaaaca tgaattaaat cagcaatcac ttctaaataa atccgctcat
     1321 ccagtaaggc tgcacctaat gctcccactt cacgatgatg gaggggacgc atggcattaa
     1381 aagtatgggt caaggatgtt aatccgacat ccgcggccgc ctgtaaatct ttggccgttg
     1441 catcggaatg gcctaaactg gctacaattc cttggctaac taagtattta actaaagctt
     1501 cgccgttttc ctcataggct aaagttactt gtttgatatt attgccactc gtcgtttgat
     1561 agtgtttaaa gctatccaca tcggcgggaa caatcgcatg ttccggttgt gcacccttat
     1621 gttttttact gataaatggt ccttctaaat gaacaccata taccaaagcg cccgcatccg
     1681 ggtgcttttt gatatattca cgaatattct ttaaagcttg gtcaatgtta tccggcgttt
     1741 gtgtcatcgt agttgctaag aaagtcgtaa ttccttccga agctaaagtc aaagcaatat
     1801 tgtgaagatc tttataagat ggatacatgg catcggaatg attagcacca tgagcatggc
     1861 gattgataaa accgggaaca acaatcaaat cttcgggtaa actcatcgca tcggccggtg
     1921 ctgaattgag gctggagatt ttcccttggt taatgctaag cgaagtttta tacaaaccca
     1981 aacctgctac ataaataaca ctatctttga atccggaaat cgtt
//
LOCUS       DAOSGN010000155         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_55623_length_2024_cov_2.856780, whole genome shotgun sequence.
ACCESSION   DAOSGN010000155 DAOSGN010000000
VERSION     DAOSGN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55623_length_2024_cov_2.856780"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1460)
                     /gene="tilS"
                     /locus_tag="PK087_02675"
     CDS             complement(138..1460)
                     /gene="tilS"
                     /locus_tag="PK087_02675"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOH18210.1"
                     /translation="MEASIKGFFEKHQLAYLHKTMVVAVSTGLDSMVLLSSLLSWRQK
                     EHFKIIVAHFNHHERAQSKEEQEFIEQFCQQQKITCFIGHLKLRDRGNFQALARKRRY
                     AFFKQVCESTKADYLLLAHHADDNVETIMMRLLRGSNLLGYAGMEAVLPFHNSTLLRP
                     FLEITKQALVDYAKQTAIKFFEDTSNQEDYYTRNRLRHHLLPMLQAEQPQYVEKFKAF
                     SDTLKGAQAVISDQVKRFVREQCTVIENETSFSKTAFLSLSKYLQTEVLFHLFKALDL
                     SKAQITSIIDGLVAPEPNWEMTLKGKLQIRREYEQIRIAPPVLKWEGQVVISRLGTTK
                     INDKYSVNVKPGEEKNLINDQEIWYNSHKLPVLIRSRRPGDKIKLAGGTKKVSDLLID
                     LKVPKQKRDQILILEKDGEILAVLGVRKSIKLQEMESCDIIMEVKKHG"
     gene            complement(1531..>2024)
                     /locus_tag="PK087_02680"
     CDS             complement(1531..>2024)
                     /locus_tag="PK087_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18211.1"
                     /translation="KNIIEYGTIFISIWTLVVITNEVLREFQKHGYRLNKFGKMLKFF
                     YIQDRTQVLYPLLIACFFLDRWYVQLLTSLYLSFLIVWKWLQKSEPTEAYGNRLKRLF
                     VMMVVIDTVTATVLHRYLPLPQLPVSVIILMMITPFMVLLGALILVPLEFLIKKGRLR
                     KRD"
BASE COUNT          622 a          431 c          351 g          620 t
ORIGIN      
        1 ccaaaaaggg aatactgcct ttgagaaggc caactaaaac cactcttttg ttgccataat
       61 cagcccgaat ttgttttcct aagcgttcgc aaatagcaac gatttcctca tgagacaaca
      121 atacttcttg tacaaaatca tccatgtttt tttacctcca taattatatc acaactttcc
      181 atttcttgaa gtttgatgct cttgcgaaca cctaaaacag cgagaatttc accatctttt
      241 tccaatatta aaatttggtc acgcttttgt ttcgggactt tcaaatcaat taataaatca
      301 ctaacttttt tggtcccacc cgctaatttg attttatcac cggggcgtcg tgaacggata
      361 agcaccggca acttgtgact attataccat atttcttggt cattgataag atttttttcc
      421 tcgcccggct ttacattaac agagtatttg tcatttatct tcgttgtccc cagccgggaa
      481 atcaccactt gcccctccca tttcaaaacg ggcggagcaa tccgaatttg ttcatattct
      541 cttctaattt gaagtttccc ttttagggtc atttcccagt tgggttcagg cgctactaaa
      601 ccatcaatga tggaagtaat ttgggctttg cttaagtcga gagctttgaa aagatggaac
      661 aagacttccg tttgaaggta ttttgataaa cttaaaaatg cggtttttga aaacgatgtt
      721 tcgttttcta ttaccgtaca ttgttcacga acaaagcgct tgacttgatc actaataact
      781 gcctgagcac cctttaaagt atcggaaaag gccttgaatt tttccacata ctgtggttgt
      841 tcggcttgca acatgggcag caaatgatgg cgaagacgat tacgagtata gtaatcttct
      901 tgattcgaag tatcttcaaa aaacttaatg gctgtttgtt tagcataatc aactaaggct
      961 tgtttcgtga tttccaaaaa cggacgtaat aatgtgctgt tatgaaaggg aaggacggct
     1021 tccatgcccg cataacccag taaattacta ccccgaagca agcgcatcat aattgtttcc
     1081 acattatcat cggcatggtg agccaaaagc aaataatcgg ctttcgtgct ctcacaaact
     1141 tgtttaaaaa acgcataccg gcgtttacga gcaagggctt gaaaattacc acgatcccgt
     1201 agctttaaat gaccaatgaa acaagttatt ttttgttgct ggcaaaattg ttcgataaat
     1261 tcttgttctt cttttgattg cgcacgctca tgatgattaa aatgggcaac aatgatttta
     1321 aaatgctcct tttggcgcca actcaacaaa ctgcttaata aaaccatgga atcaaggccg
     1381 gttgagaccg ccaccaccat cgttttatga agatatgcta attgatgttt ttcaaaaaac
     1441 cctttaatgc ttgcttccat atttttcctc attaaataat tatacactaa tctacgctac
     1501 ttacaagact aaattaaaaa gtcgtgaccc ttaatcacgc tttcttaatc ggcctttctt
     1561 gattaaaaac tccaacggca ccaaaattaa tgctcccaac aagaccataa acggcgtaat
     1621 catcattaag ataatcacag aaaccggcaa ttgaggaagc ggaagataac gatgcaaaac
     1681 ggtggctgtc accgtatcaa ttaccaccat cataacgaac aagcgtttta aacggttgcc
     1741 gtaggcttcg gtcggttcgc ttttttgaag ccacttccaa acaatcaaaa aacttaagta
     1801 gaggctcgtt aacaattgta cataccaccg gtctaaaaaa aagcaggcaa taagcagggg
     1861 ataaagtact tgggtgcgat cctgaatata aaagaatttc aacatttttc caaatttatt
     1921 taaacgatat ccgtgttttt gaaattcacg caagacttcg ttggtgataa ccaccaaagt
     1981 ccaaatgctg atgaatatcg tgccatattc aataatattc ttaa
//
LOCUS       DAOSGN010000156         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_55797_length_2020_cov_2.796438, whole genome shotgun sequence.
ACCESSION   DAOSGN010000156 DAOSGN010000000
VERSION     DAOSGN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55797_length_2020_cov_2.796438"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PK087_02685"
     CDS             complement(<1..461)
                     /locus_tag="PK087_02685"
                     /inference="COORDINATES: protein motif:HMM:NF021196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18212.1"
                     /translation="MSVLFSFIGMSDPVLNCRDAAMLHIVRHYHPAVVFLYFTKGVIK
                     RNRDRFFAKTVKALYSDIEVREIYREQLEAPHLFWQIDDDIKQILLGIHKEFPNQEIL
                     INVTSGTQQMTGSLMLVCAQLPFPVNLIQVKRPQEIDETKKDNSYLFELTTG"
     gene            complement(780..1808)
                     /locus_tag="PK087_02690"
     CDS             complement(780..1808)
                     /locus_tag="PK087_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18213.1"
                     /translation="MGFVTNSSSYSSTTINIKNKKLVELIIDYRLEYDLFVGADVHFA
                     ENEINVVFEEDGPLAVPKSTTEIVELLYLFSSDKPEDSVLLANKETINNDYEYVHWEV
                     SFSTNGFENEDEINQVWETYFRNKGEFPVDFKMLQKNTIFNFRIPKYEFKEKFIIETS
                     SKKYTYRSYEENIFDRLMSVMVKFDEYTKYQYNTQLPLQIGDKVLVQGRLSKRMGEVI
                     KINLPWKKEIYMKDVVFVVPNYEEEEINNESDDPNQNALYEVVKVVPIVNSLSEVLIL
                     TYATGDFKLEVGDLLSCDDGAFANKGLMVIRGNHFVSANNLGFDPSKLLSISKIIRKN
                     KTVDDILM"
BASE COUNT          651 a          368 c          258 g          743 t
ORIGIN      
        1 ccggttgtta attcaaacaa ataggaattg tccttttttg tttcgtctat ttcttggggt
       61 cgtttaactt gaataagatt aacaggaaac ggcaattgcg cacataccaa cattaaagaa
      121 cccgtcatct gttgtgttcc cgatgttaca ttgattaaaa tctcttggtt ggggaattcc
      181 ttgtgaattc ctaataaaat ttgcttgatg tcatcatcaa tttgccaaaa caaatgggga
      241 gcttccaatt gttcgcgata aatttctcgg acttcaatat ccgaataaag cgctttcact
      301 gtctttgcaa aaaaacgatc ccggtttcgt tttattactc ctttcgtaaa ataaagaaag
      361 acaactgcgg gatgataatg tcttacaatg tgaagcatgg ctgcatcgcg gcaatttaag
      421 accggatccg acatgccaat aaaactaaat aatacgctca ttttttaacc tacccctttc
      481 ttaaacaaat tataccatac taaaactgag catgcaacca aacattttta aaaattaatt
      541 ttgaaaagtt tttctaacta agcttatcga tctacgaata aaaaatcaat gtataattgt
      601 tattatctaa aacacaaaga caatattttt aatatcaata gttttatttt tcaatattaa
      661 actttattat ttcattcctc aaattcagca aatttaactt ttgaaatgct taaatgatgc
      721 ctcttatctt gtcatttatc aatcttatcc taagtttaat tatcttcgtc ctcttctttt
      781 tacattaaaa tatcatctac ggttttattc ttccttatta ttttacttat actaagtaat
      841 ttgctaggat caaaacctaa attgttagca gaaacaaagt gatttcctct aatcaccatt
      901 aagcctttat tagcaaaagc accatcatca catgatagca aatcgccaac ttcaagttta
      961 aaatctcctg ttgcataggt taaaattaac acttccgaca aggaattaac aatgggcacg
     1021 acttttacaa cttcataaag agcattttga ttagggtcat cgctctcatt atttatttct
     1081 tcttcttcgt aattaggaac aacaaagaca acatctttca tataaatttc ttttttccaa
     1141 ggaagattaa ttttgataac ttcacccatc cgtttcgaca accgtccttg tactaatact
     1201 ttatcaccga tttgtaaagg aagttgcgta ttgtattgat atttagtata ttcatcaaac
     1261 ttaaccatta ccgacattaa tctatcaaag atgttttctt cataactccg atatgtatac
     1321 ttttttgaac ttgtttcaat aataaatttt tctttaaatt catacttggg aattctaaaa
     1381 ttaaaaatgg tatttttttg taacatttta aaatcaaccg gaaattcccc tttgttgcga
     1441 aagtaagttt cccaaacttg attaatttca tcttcatttt caaaaccgtt tgtggaaaaa
     1501 gaaacttccc aatggacata ttcatagtca ttattaatgg tttctttatt agctaataaa
     1561 acggaatctt cgggcttatc actggaaaat aaatacaata attccacaat ttcagttgtg
     1621 gatttcggca ccgccaacgg tccatcttct tcaaaaacaa cattaatttc attctcagca
     1681 aaatggacat cggctccgac aaaaagatcg tattctaaac gataatcaat gattaactca
     1741 actaatttct tattcttgat gttgatggtt gtggaactat aactactgga gttggttaca
     1801 aagcccatta taaatttcat ctccttgctt ttttccataa aacaatgttg gtattatctt
     1861 tatttttatt ctctgtctac tttagagata tcatatcatt ttgctgtttt tgggttttca
     1921 atcgaaataa tataaaatcc aatgaaatta gtttgttttc tttaattgtt attccattat
     1981 ttttccgact tctgctggtg tatgaccaca atgttcatac
//
LOCUS       DAOSGN010000157         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_56129_length_2012_cov_2.175268, whole genome shotgun sequence.
ACCESSION   DAOSGN010000157 DAOSGN010000000
VERSION     DAOSGN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56129_length_2012_cov_2.175268"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK087_02695"
     CDS             complement(<1..251)
                     /locus_tag="PK087_02695"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOH18214.1"
                     /translation="MANNYLTVSALNKYIAYKFDTDNALRNLLIQGEISNFRISKGHL
                     YFSLKDEESEIRAIMFGSSAKVLNFIPVDGMMVLIKASVT"
     gene            complement(339..656)
                     /locus_tag="PK087_02700"
     CDS             complement(339..656)
                     /locus_tag="PK087_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOH18215.1"
                     /translation="MKRNNSRIKAMIVLYNYDLLGEKPNYEYLNQIIQEEEEVSLDED
                     FFNELVDGVINNWDEINRIFSRNLKNWTFERMSIVDRNLIRIAIYKCFTLKPRPPLLS
                     MKF"
     gene            complement(764..1120)
                     /locus_tag="PK087_02705"
     CDS             complement(764..1120)
                     /locus_tag="PK087_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18216.1"
                     /translation="MISDPANDGYLLGEYLLEQIIIGNQNTMKLRFFTYSFIIGVCFT
                     AITILILWVFFHRDGKFKRFSEYYNIGAIASIPVTLVFFVLLWFFPKLLDFYIFVFSL
                     YYLIVISTINNDEQLV"
     gene            complement(1145..1738)
                     /locus_tag="PK087_02710"
     CDS             complement(1145..1738)
                     /locus_tag="PK087_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18217.1"
                     /translation="MKFKDILKSLILPRWMVKYKSMSIIIAICIFAISSFIIALPPSQ
                     NKTLNEQDILNNYNFNVLSEFPNTAIVNNVIKQIVDKECAVVDGKELKCGQMEAVDNF
                     ETDFSFVEDGITKNIHFVIDLFDIKKVYLEDEKIYYDVEKRFNIEKIPYQENHENYLI
                     VFYSDALYFQAHPFAIDSLNINHKGHKLVPTTKKIFY"
     gene            complement(1758..>2012)
                     /locus_tag="PK087_02715"
     CDS             complement(1758..>2012)
                     /locus_tag="PK087_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18218.1"
                     /translation="NTLLICVLFPFLMILIFWLFFRRNGRLTQFREYFNIAALSSVVP
                     FLITFAVSCFFQPILNAYIFIFSIYYLFILYRINNIQTDL"
BASE COUNT          768 a          310 c          294 g          640 t
ORIGIN      
        1 gtcacagaag ctttgatgag aaccatcatt ccgtcaacag gaataaagtt tagaactttg
       61 gcactggagc caaacataat agctcgaatt tccgactctt cgtcttttaa tgaaaaataa
      121 agatgacctt tggaaatgcg gaaattggat atttcccctt gaattaacaa atttcttaaa
      181 gcattatcag tatcaaattt ataagcaatg tatttgttta aagccgaaac agttaaataa
      241 ttatttgcca ttgatgctct tcctaatttc atctaatatt ttgttattaa atttggatga
      301 caatccatca tcaagttcac tatattcatg ggttaaattc aaaatttcat tgataataat
      361 ggtggccggg gttttagtgt aaagcatttg taaatggcaa ttctaattaa attacgatcg
      421 acaatcgaca ttctttcaaa agtccaattt tttaaattgc gactaaaaat tcggttaatc
      481 tcatcccagt tattaatcac cccatcaact aattcattaa aaaaatcttc atccaaggac
      541 acttcttctt cctcttgaat gatttgattt aaatattcat aattgggctt ttctcctaac
      601 aaatcataat tatataaaac aatcatggct ttgattcgac tattgtttct cttcatgttt
      661 acacctaaca attagtattt tagcataaat ccactttttt taaaaggaaa ataaccaaga
      721 acaggcaata aaaatcccca attaaacaat tgaggattaa tgcttaaact agttgctcat
      781 cattattaat ggttgaaata actattaaat aataaagtga gaaaacgaaa atataaaaat
      841 ctaacaattt gggaaaaaac caaagcagaa caaaaaagac taaagtaacg ggaatgctag
      901 caatggcgcc aatattataa tattctgaga agcgtttaaa ctttccatcg cgatgaaaaa
      961 acacccaaag aatgaggata gttatggccg taaagcagac accaataatg aagctatagg
     1021 taaaaaaccg gagtttcatc gtattttgat tgccaatgat gatttgctca agcaagtatt
     1081 cacctaataa ataaccatcg ttggcggggt cagatatcaa aagttgaaag ttattaatgc
     1141 tatcttagta aaatattttt ttggtagtgg gtaccaattt gtgaccctta tgattaatat
     1201 ttaaactatc aatagcaaag ggatgggctt gaaaatataa agcatcggaa taaaaaacga
     1261 ttaaataatt ttcatgattt tcttgatagg gaattttttc aatattaaaa cgtttctcaa
     1321 catcataata aattttttca tcttctaaat aaaccttttt tatgtcaaac aagtcaatca
     1381 caaaatgaat atttttggta attccgtctt caacaaacga aaaatcagtt tcgaaattat
     1441 caacggcttc catttggcca cattttaatt cttttccatc gacaaccgca cattctttat
     1501 cgacaatttg tttgataaca ttgttaacaa tggcggtatt cgggaattcg cttaagacat
     1561 taaaattata attgtttaaa atatcttgtt cgttcaaagt tttgttttga cttggtggta
     1621 gagcgatgat aaaagaagaa atggcaaaaa tacaaatggc gataatgata ctcatcgatt
     1681 tgtatttcac catccatctt ggtaaaatta atgatttcaa aatatcctta aatttcataa
     1741 tgcccctcaa taatgattta taaatcagtt tggatgttat tgatacgata aagtatgaat
     1801 aaataataga tggaaaaaat gaaaatataa gcgtttaaaa ttggttgaaa aaagcaagaa
     1861 acagcaaaag taattaaaaa cggaacaaca ctacttaaag cagcaatatt aaagtattca
     1921 cgaaattgcg tcaaacgacc attgcgtcta aagaacaacc aaaaaatgag aatcatcaaa
     1981 aagggaaaaa gaacacaaat taacagggta tt
//
LOCUS       DAOSGN010000158         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_56330_length_2007_cov_2.411373, whole genome shotgun sequence.
ACCESSION   DAOSGN010000158 DAOSGN010000000
VERSION     DAOSGN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56330_length_2007_cov_2.411373"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PK087_02720"
     CDS             complement(<1..403)
                     /locus_tag="PK087_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004012180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOH18219.1"
                     /translation="MADEKLKALEDAFKQIEKQFGKGSIMKLGEAVFGDMEVIPSGSI
                     SLDLAMGVGGYPKGRIVEIYGPESSGKTTLALHAIASAQKAGGYAAFIDAEHALDPVY
                     AKNLGVDINNLILSQPDTGEQALEIAEHLIRS"
     gene            complement(413..1033)
                     /gene="pgsA"
                     /locus_tag="PK087_02725"
     CDS             complement(413..1033)
                     /gene="pgsA"
                     /locus_tag="PK087_02725"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOH18220.1"
                     /translation="MNLPNRLTLARICLIPFIIFIPLVPFFNNVVLFGDVKLNTQFTL
                     ENLIVLIIFVVGAFTDFLDGYIARKHHLITDFGKFMDPLADKLLVLAALIVLLERGRM
                     EAFGFSLGFVVTLILAREFMVTGIRLIAASDHLVIAASKLGKFKTISQMALIIILLLN
                     GYPFTLLGSLAQDIVSLTLIVLAGGLTIISGVDYFWKNKDIILKNK"
     gene            complement(1086..>2007)
                     /locus_tag="PK087_02730"
     CDS             complement(1086..>2007)
                     /locus_tag="PK087_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOH18221.1"
                     /translation="SEVQAFFQSCGLFLKTDGSTRLYPYSEQATTVLEVLRYELKRHH
                     IGTICNVRVKSLIYEQGWIVKTNQRDFKADYVVVASGSLAQEQTEGYRILQELGHRLI
                     PLVPGLAALKTKEKLRHLQGLRVKCQAGIYEQKRLLHQDKGEILFKDDGLSGILAFDL
                     SRFLTKGNEVALDLLFDYPHLERELTSLLTWKELEDALMGLLPKMLVWEILKRNPNRD
                     IKAIVKTIHHLSFEVVATYGFEWAQIARGGISLEDINLDFSSRKQAHLSIIGEVLDVD
                     GASGGYNLHFAWLSGMIAGRAIASFEKNGK"
BASE COUNT          622 a          467 c          352 g          566 t
ORIGIN      
        1 tactgcgaat taagtgttcc gcaatctcca gcgcctgttc accggtatcc ggctgcgata
       61 aaattaaatt attgatatcc acgcctaagt tcttcgcata tacgggatct aaggcatgtt
      121 ccgcatcaat gaaggcggca tatcctccgg ctttttgggc cgaggcaatg gcgtgaaggg
      181 ctagggttgt tttcccacta ctttcaggac catatatttc aacaatccta cctttcggat
      241 aaccaccgac ccccatggcc aaatccagtg aaatcgagcc cgacgggata acttccatat
      301 cgccgaacac cgcttcaccc agtttcataa ttgagccttt cccaaattgt ttttcaatct
      361 gcttaaaggc atcttctaaa gcttttaatt tttcgtctgc cattttttac tcctatttat
      421 tctttaagat aatgtctttg tttttccaaa aataatctac gcccgaaata atcgtcagtc
      481 cacctgctaa aacaatgagc gttaaactga cgatatcttg cgccaagctt cctagtaatg
      541 tgaaaggata accgttgagc aacaagataa tgatgagagc catttgggaa attgttttga
      601 atttacccag tttgctggca gcaatcacta aatgatcgct tgcggcaatg aggcgaatcc
      661 ctgtcaccat aaattcccgg gctaaaatta aggtgaccac aaagccgagg ctgaagccaa
      721 aagcttccat tcttcctcgt tctaacaaaa caattaaagc cgccaacacc aataatttat
      781 ccgccaaggg atccataaat ttcccaaaat ccgttattaa atgatgcttg cgggcaatgt
      841 aaccatccaa aaaatcggta aacgccccca ccacaaagat aatcaataca attaagtttt
      901 ctaatgtaaa ttgggtgttg agcttgacat cgccaaataa aacaacatta ttaaaaaaag
      961 gaaccaaagg aataaaaata atgaacggaa tgagacaaat tctagctagt gttaaacgat
     1021 taggtaaatt catattattc acactttctc ttgtttatta tacaatattt tgtcatttta
     1081 gtaaactatt tcccgttttt ttcaaaagaa gcaatcgccc gaccggcaat catcccactt
     1141 aaccaagcaa agtgtaaatt gtagcccccg gacgccccat ccacatccaa gacttcaccg
     1201 ataatagaga gatgtgcttg ttttcgagaa gaaaaatcaa ggttgatatc ttcaagacta
     1261 ataccgccac gagcaatctg cgcccattca aagccgtaag tagcaacaac ttcaaaactt
     1321 aaatgatgga tggtttttac aatcgcttta atatcacgat taggatttct ttttaaaatc
     1381 tcccatacca acattttcgg caaaagcccc attaaagcat cttctagttc tttccaagta
     1441 agtaagcttg ttaattccct ttccaaatgg ggataatcaa aaagcaaatc aagcgctact
     1501 tcgtttcctt ttgtaagaaa acgagataaa tcaaaggcca aaatgcccga taaaccatca
     1561 tctttaaaga ggatttcccc tttgtcttga tggagaagtc gcttttgttc gtaaatacca
     1621 gcttggcatt tgactcttaa accttgcaag tggcgaagtt tttctttcgt cttaagagcc
     1681 gccaagccgg gaacaagggg aattaagcgg tgtccaagct cttgaagaat gcggtagcct
     1741 tccgtctgct cttgagctag ggagccactc gccactacca cataatcagc cttaaaatcg
     1801 cgttgattgg ttttcacaat ccacccctgc tcataaatta aagacttgac cctcacattg
     1861 caaatcgtgc caatgtgatg gcgttttaat tcataacgca aaacttccaa caccgttgtg
     1921 gcttgttcgc tataaggata taagcgtgtt gaaccgtcgg tttttaagaa caaaccgcaa
     1981 ctttggaaaa aagcctgaac ctcacta
//
LOCUS       DAOSGN010000159         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_56591_length_2001_cov_2.712744, whole genome shotgun sequence.
ACCESSION   DAOSGN010000159 DAOSGN010000000
VERSION     DAOSGN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56591_length_2001_cov_2.712744"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /gene="ruvB"
                     /locus_tag="PK087_02735"
     CDS             <1..695
                     /gene="ruvB"
                     /locus_tag="PK087_02735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOH18222.1"
                     /translation="LFIDEIHRLPRVVEEVLYSAMEDFVVDIMIGKDATANSIRIDLP
                     PFTLIGATTRCGDLSAPLRDRFGIVHQLNYYQTSELKEIVNRTSRVFDFQIDEQAAWE
                     IARRSRGTPRVANRLFRRVRDFAQFEDEFCQVIAYELTKSSLVKLDIDEEGLNETDHR
                     YLRSLVERFKGGPVGLGALASSISEDVTTLEDVYEPYLLQEGFILRTSRGRVATEKTY
                     RHLGLGYEGRLF"
     gene            676..1695
                     /gene="queA"
                     /locus_tag="PK087_02740"
     CDS             676..1695
                     /gene="queA"
                     /locus_tag="PK087_02740"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003137800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOH18223.1"
                     /translation="MKVDSFDYQLPKQIIAQTPLEKRSSSKLLVLNRCLGTMEDHYFR
                     ELPAFLTENDVLVLNDTKVLPSRIIGQKVDTQAKIELLLLKEEADYWETLVRPARRIK
                     VGTMIDFASLFTGEILAKFNDGLCHVRFHYQGVFLDLLSKLGTMFFPSYIHTTLKDPN
                     RYQTIYAKTLGSAAAPTAGLHFDDEVFKQLRAKKVRIAYVSLHIGLGTFRSVSVENVE
                     DHQMHSEWFSMPKETADLLNAAKQNRQRIICVGTTSTRTLEAIYQKYHRFVATTEATN
                     LFIYPGYRFLAVDALITNFHLPKSTLLMLVSAFYSQEKIMDAYQYAITHDYRFFSFGD
                     AMFIQ"
     gene            1698..>2001
                     /locus_tag="PK087_02745"
     CDS             1698..>2001
                     /locus_tag="PK087_02745"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013727736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOH18224.1"
                     /translation="MFMLIKYELIKKDRTSRARLGLITTPHGQFATPVFMAVGTQGTV
                     KTLIKEELEAIGAEIILGNTYHLWNQPGPDIIKQAGGLHRFMNWDRSLLTDSGGFQV"
BASE COUNT          543 a          395 c          440 g          623 t
ORIGIN      
        1 ttttgttcat tgatgaaatt catcgtcttc ctcgggttgt cgaggaagtg ctttattccg
       61 caatggaaga ttttgtcgtt gatatcatga ttggcaaaga cgcaacggcc aattccattc
      121 gcattgatct tcctcctttt accttaattg gagcaacaac gcggtgtggt gatttatcgg
      181 cgcccttacg cgatcgtttt gggattgtcc accaactcaa ttattatcaa acttcggaat
      241 taaaagagat tgttaaccgt acttcgcgtg tttttgattt tcaaattgat gaacaagccg
      301 cttgggaaat tgctcgccgt tcgcggggaa caccgcgggt tgccaatcgt ttgtttagac
      361 gcgttcgcga ttttgcccag tttgaagatg aattttgcca agtgattgct tatgagttaa
      421 caaaatcttc tttagtaaaa cttgatattg atgaagaggg attaaatgaa accgatcatc
      481 gctatttgcg ttcccttgtg gaacggttta aggggggacc ggttggtctt ggagctttag
      541 cttcatcgat ttctgaagat gtgacgaccc tcgaagatgt ttacgaaccc tatttattac
      601 aggaaggttt tattttacga acttcacggg gaagagtggc caccgaaaag acataccgcc
      661 atttgggatt gggttatgaa ggtcgactct tttgattatc aattacccaa gcaaataatt
      721 gcccaaacac ctttggaaaa acgtagttct tccaaattgt tagtcttaaa ccgttgttta
      781 ggaacgatgg aagatcatta ttttagggaa ttaccggctt ttttaacaga aaatgatgtc
      841 ttagtgctca atgacacgaa ggttttacct tcacggatta ttggccaaaa agtagatacg
      901 caagctaaaa tagaattgtt acttttgaaa gaagaagcgg actattggga aacattagtt
      961 cgcccggcaa ggcgtatcaa agtggggacg atgattgatt ttgcatcgtt atttacggga
     1021 gaaatcctgg cgaagtttaa tgatggtctt tgccatgtcc gctttcacta tcaaggagtg
     1081 tttttagatc ttttgtctaa actgggaacg atgttttttc cctcctatat tcacaccact
     1141 ttgaaggatc ccaatcgtta tcaaaccatc tatgctaaga ccttgggcag tgccgctgca
     1201 cccacagcgg ggcttcattt tgatgatgaa gtttttaagc aattacgggc taaaaaagta
     1261 aggattgctt atgtaagctt gcatatcggt ttagggactt ttcgcagtgt cagtgttgag
     1321 aatgtggaag accatcaaat gcatagcgaa tggttttcga tgccgaaaga aacggccgat
     1381 ttattaaatg cggctaaaca aaatcgtcag cgcattattt gtgtcggcac cacttccaca
     1441 agaactttag aagccattta ccaaaaatat caccgctttg tagcgacaac cgaagcaacc
     1501 aatcttttta tctatccggg atatcgtttt ttggcagttg atgctttgat tacgaatttt
     1561 catcttccca aatcaacgct tttgatgctc gttagtgctt tttactccca agaaaaaata
     1621 atggatgcct atcaatatgc gattacacac gactatcgct ttttctcatt cggtgatgcg
     1681 atgttcatcc agtaaggatt tttatgctta ttaaatatga attaattaaa aaagaccgta
     1741 cttccagggc ccgtttgggt ttgattacca ccccccatgg gcaatttgcg acgccggtat
     1801 ttatggctgt cggcacccaa ggcaccgtta aaaccttaat caaagaagaa ctggaagcca
     1861 ttggggcgga aatcatttta ggaaatacct accatttatg gaatcaaccc gggcccgaca
     1921 ttattaagca agccggaggg cttcatcgct tcatgaattg ggatcgctct cttttgactg
     1981 attcgggtgg ttttcaggtc t
//
LOCUS       DAOSGN010000160         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_56731_length_1998_cov_2.326814, whole genome shotgun sequence.
ACCESSION   DAOSGN010000160 DAOSGN010000000
VERSION     DAOSGN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56731_length_1998_cov_2.326814"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK087_02750"
     CDS             <1..180
                     /locus_tag="PK087_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOH18225.1"
                     /translation="PYYYAYLVYFFMLSCGISAALLGVNPYDQPGVEAYKRNMFALLG
                     KPGLEKLTEELKKKR"
     gene            177..728
                     /locus_tag="PK087_02755"
     CDS             177..728
                     /locus_tag="PK087_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18226.1"
                     /translation="MKKTIILILLVLLGSLSGCQKNRVEDDYPMLNGINHVFEKSTYQ
                     EVTKALTVASGVQVVLFSYNPHFYECPFCLAVMPIINDAALKMGIEKILYLDIYEMRK
                     NNTTEYQLLLGYLDNKVGDLIVRDGVKKLIVPDLYVVKDGEIAAHHIATLKNDKGAFI
                     YDLSPEQKQELQTIYEQMFQLVL"
     gene            838..1287
                     /gene="tsaE"
                     /locus_tag="PK087_02760"
     CDS             838..1287
                     /gene="tsaE"
                     /locus_tag="PK087_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOH18227.1"
                     /translation="MKIKKIIANEQMMINLGQIIGEHAQANMVIALNGNLGVGKTTLT
                     KGIALGLGIRETINSPTFTILKIYKGRLPLYHLDLYRTSMGSGDEDLAEYFEQGGLCV
                     VEWANNIAHLLPANCLTITFFVLSNGARQVDLSTDNPAFNQMLGAIK"
     gene            1284..1859
                     /gene="tsaB"
                     /locus_tag="PK087_02765"
     CDS             1284..1859
                     /gene="tsaB"
                     /locus_tag="PK087_02765"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOH18228.1"
                     /translation="MRTMILDTSTALMYVAFVQNNQVIYEKKQFGKNNHSEHFLLAIE
                     EGLKHLSWQVKDFGAIIVGIGPGSYTGLRISLTVAKIWAWTLQIPLYTVSSLDLLASG
                     YYHQNGLYAVVSLAKSGHVYGKVLKAQDGSIRPFGEESFMEKTAFLDSIKHLNYSLVD
                     EQNYLVDIRGLKPELVQDLHNLSPYYMRRGI"
BASE COUNT          601 a          346 c          433 g          618 t
ORIGIN      
        1 ccctattatt atgcttatct agtttatttc ttcatgctta gctgtggcat cagtgcggct
       61 ctattaggtg ttaatcctta cgatcaaccg ggggttgaag cttataaacg aaatatgttt
      121 gccttgcttg gaaaaccggg cttagaaaaa ctgacggagg aactaaagaa gaagcgatga
      181 aaaaaaccat cattttaatt ttactagtgt tgttgggctc attaagcggc tgtcaaaaga
      241 atagggttga ggatgattat ccaatgctta atggaattaa ccatgttttt gaaaaaagca
      301 cttaccagga agttacaaag gctctcacag ttgcttccgg ggttcaggtt gttttatttt
      361 cctataatcc ccatttttat gaatgtccgt tttgtctcgc cgtcatgccg attattaatg
      421 atgcggcttt aaaaatgggg atcgagaaaa ttctttattt agacatctat gaaatgcgta
      481 agaataacac gacggaatat caattgttgc tcggttattt agacaacaaa gttggcgatt
      541 taattgtccg tgatggtgtt aaaaaactga ttgttcccga tttatatgtt gtaaaggacg
      601 gcgaaattgc cgctcatcat attgcaacat taaagaatga caaaggtgca tttatttatg
      661 atttaagccc ggaacaaaaa caagaacttc agaccattta tgaacaaatg tttcaattag
      721 tgctctaata atcgaaaaat caaataaggc gattatggct atatttgatt ttttttgttt
      781 gtgagtagtg aaatatcctc cggggtatgg tataataata caaaaatagg aggcaatatg
      841 aaaatcaaaa aaatcattgc taacgaacag atgatgatta atctcggtca aatcatcggc
      901 gaacatgccc aagctaatat ggtcatcgcc ttaaacggca atttaggagt cggaaaaacg
      961 actttaacta aaggtattgc tttgggcttg gggattaggg aaaccatcaa cagccccact
     1021 ttcacaatcc tcaaaatcta caaaggtcgt ttgcctttat atcaccttga cctctatcgg
     1081 actagtatgg ggagcgggga tgaggattta gcggagtact ttgaacaagg tggcctttgt
     1141 gttgtggaat gggccaataa tattgctcat ttactcccgg ctaattgtct aacaatcacc
     1201 ttttttgttt taagtaatgg tgcaagacaa gttgatttgt cgacggataa tccggccttt
     1261 aatcaaatgc ttggggcgat aaaatgagaa cgatgatttt ggatacatcg acagccctta
     1321 tgtatgtcgc ttttgttcaa aacaatcaag taatatatga aaaaaaacag ttcggaaaaa
     1381 acaaccattc cgaacatttt ctgctggcca ttgaagaagg actgaagcac ttgtcatggc
     1441 aagtcaaaga ttttggggcg attattgtcg gtatcggccc cggttcttat accggcttac
     1501 gtattagttt aacggttgca aagatatggg catggacact gcaaattccc ctttatactg
     1561 ttagcagctt ggacttatta gctagtggct attatcatca aaacggtctt tatgctgtcg
     1621 tgtcgcttgc aaaaagcggc catgtttatg gcaaagttct taaggcgcaa gacggtagca
     1681 ttcgcccttt tggggaggaa agttttatgg aaaaaacagc ctttttggat agcatcaagc
     1741 atcttaacta tagcttagtg gatgaacaaa attatttagt tgacatccga ggccttaaac
     1801 cggaactggt gcaggatttg cataatttat cgccctatta tatgcggagg gggatatgaa
     1861 atatcaaatc cggcctttgg aagaaaaaga tatcggggca atcgtggccg gtgaagaagc
     1921 catttttggt gtttccttag gctttgatct tatttatgcg gattttaaac tcaatccctt
     1981 tgctcattac ttggtttt
//
LOCUS       DAOSGN010000161         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_57281_length_1986_cov_1.818229, whole genome shotgun sequence.
ACCESSION   DAOSGN010000161 DAOSGN010000000
VERSION     DAOSGN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57281_length_1986_cov_1.818229"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PK087_02770"
     CDS             complement(<1..607)
                     /locus_tag="PK087_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HOH18229.1"
                     /translation="MNLAAIYHLPKSHMAYAYDQETLHLYLQVGKNDATKVTLIAGDP
                     FDYRLENGVYLWNGRTKPAIKMEKVLSNKYHDFWFVSLKVPTKRQKYAFLIHGLKSTY
                     FYGSRHLQKVTSKTPPDSLYVLFDYFNYPYINDQDLFVAPAWTKNTIWYQIFPERFSR
                     SEKIPGTYLPWGSIEQGITNQQFFGGNIPGIIEKLAYLHDLG"
     gene            complement(604..1386)
                     /locus_tag="PK087_02775"
     CDS             complement(604..1386)
                     /locus_tag="PK087_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOH18230.1"
                     /translation="MSKLDYFQVSIDTLNRKRTIRVYLPPGYFQSANKYDVLYMHDGH
                     NLFQVETSAYGKIWDVKNTLDAIKHKDYRGLIVVGIDCDPQFRLNEYSPWLMEASLFR
                     RKLALGNNLGGEGEQYVAWLVNKLIPLIKQKYRTTGINYLAGSSMGALITLYAGLRYP
                     FIFSKVGCFSSAFWFARKQLTAFIKKTPPTNLHIYLDVGSKETKSILQNYYYVHGSQV
                     INRLLKKQGFKNTQLLIEKGGNHSEEAWARRFPGFIEWLLQR"
     gene            1546..>1986
                     /locus_tag="PK087_02780"
     CDS             1546..>1986
                     /locus_tag="PK087_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOH18231.1"
                     /translation="MNFDRRLRAYSIDSSLAFTLMLIGMIVIYNLPSILPLGKMAIAL
                     GCYFGVMIIPHFFSPGQTFGKRIQKLKVVKNYQELIPEKVFVPALYLLILREIVKALL
                     TFITFGVYIFIAGLVASGREDGRTIHDLIFKTRVIALTKYTSDRI"
BASE COUNT          578 a          370 c          404 g          634 t
ORIGIN      
        1 tccctaagtc gtgtaaataa gcgagttttt cgataatgcc gggaatattt cccccgaaga
       61 actgctggtt agtaatccct tgttcaatgc ttccccatgg caagtaagtt ccgggaattt
      121 tctcggagcg agaaaagcgt tccggaaaga tttgatacca aatggtattt ttcgtccatg
      181 ccggcgcaac aaataaatct tggtcattga tataaggata attaaaatag tcaaacaaaa
      241 catataagga gtcgggtggg gttttggaag tcactttttg taaatggcgg ctaccataaa
      301 aataagtgct ctttaagcca tgaatcaaaa aggcatattt ttggcgtttg gtgggcactt
      361 ttaagctcac aaaccaaaaa tcatggtatt tattggataa tactttttcc atcttaatgg
      421 ctggcttggt gcgaccgttc caaagataga caccgttttc caagcggtaa tcaaagggat
      481 cgccggcaat caaagtaact ttggtggcat cattttttcc tacttgtaaa tataaatgca
      541 gggtttcttg gtcgtaggca taagccatat ggcttttagg aagatggtaa atggccgcaa
      601 gattcatcgt tgtaataacc attcgataaa acccggaaac cggcgggccc aggcttcttc
      661 cgaatgattg cctccttttt caattaataa ttgggtgttt ttaaaaccct gtttttttaa
      721 taagcggttt atcacttgac taccatggac atagtaataa ttttgtaaga tgctcttggt
      781 ttctttggag ccgacatcta agtaaatatg tagattcgtt ggcggtgttt ttttaatgaa
      841 tgccgtcagt tgttttctag caaaccaaaa ggcactggaa aaacaaccga ctttagaaaa
      901 tatgaaagga tatcgtaggc cggcgtaaag ggtaatcaaa gcccccattg aggaaccggc
      961 cagataattg atgcccgtag ttcggtattt ttgcttaata agaggaatga gtttattaac
     1021 caaccatgcc acatattgtt caccttcgcc tccaagatta tttcctaaag cgagttttct
     1081 gcgaaaaagc gacgcttcca taagccaggg gctgtattca ttgaggcgga attgcggatc
     1141 acaatcaatg ccaacaacga ttaaaccgcg ataatccttg tgcttgatgg catcaagggt
     1201 gttcttaaca tcccagattt ttccgtaagc actggtttca acttgaaaca agttatggcc
     1261 atcatgcatg tacaaaacat cgtatttatt tgcggactga aaataaccgg gtggcaaata
     1321 aactcggata gtacgcttgc ggtttaaagt atcaatgctt acttggaaat aatctaactt
     1381 actcataact aacctcactt aaattataac ataagttctt tttttaaaat atagggttta
     1441 taagaaacgg tttccatctt tttttgcttt cagtattacc gaggaaagca attgacaaac
     1501 cgcttgtttc ctagtaaact tgaagataag aaaagaggaa agcttatgaa ttttgatcgc
     1561 cgattgcggg cttattcaat cgattcatca ttagccttta ctttaatgct tattggtatg
     1621 attgtcattt ataatcttcc tagcatcttg cctttaggta aaatggccat tgctttgggg
     1681 tgttattttg gggttatgat tattcctcat ttcttttcgc ccggccaaac tttcgggaaa
     1741 cgcattcaaa aattaaaggt tgtgaaaaac tatcaggaac taattcccga gaaggtgttt
     1801 gttccggcgc tttatttatt aatcttgcgt gaaattgtta aagcactttt aacttttata
     1861 acatttggcg tctatatttt tatagcgggg cttgttgctt ccggtcgcga agatggcaga
     1921 accatccatg atttaatttt taaaacccgg gtgattgctt taaccaaata tacctcggat
     1981 cgcatc
//
LOCUS       DAOSGN010000162         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_57629_length_1978_cov_2.382735, whole genome shotgun sequence.
ACCESSION   DAOSGN010000162 DAOSGN010000000
VERSION     DAOSGN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57629_length_1978_cov_2.382735"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    complement(74..263)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(443..1291)
                     /locus_tag="PK087_02785"
     CDS             complement(443..1291)
                     /locus_tag="PK087_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HOH18232.1"
                     /translation="MNNYLGTIQKYRVLRETKLGYVLTLNGNDEYFLHRNETNFQKLA
                     LDDEIEAFLYADKKGRLALTLFKPTATVHKIGFARVQDVKQQLGVFLNIGTSKDILLS
                     KDDLPINHVEWPQCGDYIIGKLRVKSEKLILKMASKEEILKQKQAKELPLNEKVKAFV
                     YRITPDGVNLVTENWNIVFVYHTNLRKKYRLGEEVMVKVLSKNETDYTGTLIDQKEIM
                     IKTDRDVLLAYLKKHHGVMAITEKSDAEAIKKVFNMSKKAFKAALGNLYKDRLIEIHD
                     DKIILL"
     gene            complement(1284..>1978)
                     /locus_tag="PK087_02790"
     CDS             complement(1284..>1978)
                     /locus_tag="PK087_02790"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18233.1"
                     /translation="DQLLWVLPIEDMLGVGKKTAPKLKMLGINTIGDLANFTDVELLK
                     RTIGEVNTLSLREKAWGIDDTFVDYSNDDSVQSISHAHTFDHDLYDPMIIKKTLKVLA
                     NAVSYRLQKRQYRALTVGIQIKYADFHQINRSKSLNKPVQNEVDIYSIVEDLFDDLFV
                     TGDLIRLVGVFANRFSQDEGKEKQVSLFDDLTKIEKENEIQKIILGVQNTFGKDTIKF
                     GYYERGKKDKNE"
BASE COUNT          626 a          374 c          288 g          690 t
ORIGIN      
        1 aatcttgggt tcccttaact ttttgataat tcattttaac ctcctgaaaa ataaaaatcc
       61 ttaaaagaat acttctaagg acgatttacc gtggtgccac cttattcatc aattattgat
      121 gcacttaatt ctttaacgca agaaatacgt ccactaagga atctcataag tgtctttcat
      181 taacggaata tcaagtgctc acactaacca ctttcgctga agatatttaa acatcaatta
      241 cttttcttac tcaccgaatt atgaatttaa gttattataa ttgaaaattg acagaatgtc
      301 aatacactga tacactggcc tttggtggag ccttcccaaa tcgcgcaaaa gataattctt
      361 cgacataaaa aagataatcg tctcaaaaat cgtcgccaac atgcttaatt atactcaaaa
      421 aagcagattg aggttgttta aattacaata aaatgatttt atcgtcatga atttcaatta
      481 atcgatcttt atacaaatta ccgagagcgg ctttgaaggc tttcttactc atgttaaaaa
      541 cctttttgat ggcctcagca tcactctttt cagtgattgc catgacccca tgatgttttt
      601 ttagataagc caaaaggaca tcacgatcag ttttaatcat aatttctttt tggtcaatca
      661 aagtaccagt atagtcagtt tcatttttac ttaagacttt taccattact tcttcaccta
      721 agcgatattt ttttcgaaga ttagtatggt aaacaaaaac aatgttccag ttctcggtga
      781 ctaaattaac tccatccgga gttatccgat agacgaaagc tttaactttt tcgttaagtg
      841 gcaattcctt tgcttgcttt tgttttaaaa tttcctcttt gcttgccatt tttaaaataa
      901 gtttttcgga tttcactctt aatttaccaa tgatatagtc accacattgg ggccattcca
      961 cgtgattaat tggcaagtcg tctttggaaa gtaagatatc cttactcgtc ccgatgttta
     1021 agaaaacgcc aagttgttgt ttgacatctt gaacgcgagc aaaaccgatt ttatgaactg
     1081 ttgcagtcgg tttaaagagc gttaacgcta agcgaccttt tttatcagca tataaaaaag
     1141 cctcaatctc atcatcaaga gccaattttt ggaaattcgt ctcatttcga tggagaaaat
     1201 attcatcgtt accgttgagc gttaatacat atcctagttt tgtttctcgt aaaactcgat
     1261 atttttgaat ggtccctaaa taattattca tttttatcct tcttccctcg ctcgtaatat
     1321 ccaaatttaa tagtatcttt cccaaaggtg ttttgaacac caaggataat tttttgaatt
     1381 tcgttttctt tctcgatttt tgttaagtca tcaaaaagag aaacttgttt ttctttgcct
     1441 tcgtcttgag aaaaacgatt ggcaaagacg ccaactaaac gaattaaatc accagtaacg
     1501 aataaatcat caaataaatc ttcaacaata ctatagatat caacttcatt ttggacaggt
     1561 ttatttaaac ttttactacg gttaatttga tgaaaatcag catatttaat ttgaatgccc
     1621 accgttaatg ctcggtattg tcttttttgt aaacgataag aaacggcatt agctaatact
     1681 ttaagggttt ttttaataat catcgggtca tacaaatcat gatcaaaggt atgagcatgg
     1741 ctgattgatt ggaccgaatc atcattggaa taatcaacga atgtatcatc aatgccccaa
     1801 gctttttccc gtaaacttaa agtattaact tcaccaatag ttcgtttcaa taattcaaca
     1861 tcggtaaaat tcgctaaatc cccaatcgta tttattccaa gcatttttaa tttaggggca
     1921 gtttttttcc caacaccgag catatcttca attggtaaca cccataaaag ttgatcga
//
LOCUS       DAOSGN010000163         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_57797_length_1974_cov_2.836373, whole genome shotgun sequence.
ACCESSION   DAOSGN010000163 DAOSGN010000000
VERSION     DAOSGN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57797_length_1974_cov_2.836373"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PK087_02795"
     CDS             <1..737
                     /locus_tag="PK087_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOH18234.1"
                     /translation="PRIQELFEAREPKGKAIISEIDGLVTNIRHERDNRFEVTITNKE
                     VNDEKKYLTDSGKVPIVHDKDRIKAGDRITEGLISPKELLRVGTVEAVENYILKEVQK
                     VYRSQGVEIADKHIEIIIKQMMQKVAIIHEGGTDLLPGSSISKAELYDYVQACIKEGK
                     DVPIAKSVLLGITRASLKSDSFLSAASFQETTRVLTEAAIRGKRDSLEGLKENVIIGG
                     LIPAGTGLIDADFIEVPDLEEEEEEI"
     gene            875..1285
                     /gene="rpsL"
                     /locus_tag="PK087_02800"
     CDS             875..1285
                     /gene="rpsL"
                     /locus_tag="PK087_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOH18235.1"
                     /translation="MPTINQLVRKPRESKKRKSKSPHLSVSFNTLKKTYTEVKSPQKR
                     GVCTRVATLTPRKPNSAIRKYARVRLSNGIEVTAYIPGIGHRLQEHSVVLIRGGRVKD
                     LPGVRYHIIRGTQDATGVEKRVQGRSKYGAKKPK"
     gene            1313..1782
                     /gene="rpsG"
                     /locus_tag="PK087_02805"
                     /pseudo
     CDS             1313..1782
                     /gene="rpsG"
                     /locus_tag="PK087_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297750.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
     gene            1796..>1974
                     /locus_tag="PK087_02810"
     CDS             1796..>1974
                     /locus_tag="PK087_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOH18236.1"
                     /translation="MPREISLDKTRNIGIMAHIDAGKTTVTERILFHTGKIHKMGETH
                     DGAATMDWMEQEMERG"
BASE COUNT          669 a          348 c          444 g          513 t
ORIGIN      
        1 taccgcgaat tcaggaattg tttgaagctc gagaaccaaa aggaaaggcg attatttccg
       61 aaattgacgg ccttgtcacc aatattcgtc atgaaagaga caaccgtttt gaggttacga
      121 ttaccaataa agaagtcaat gatgagaaaa aatatttaac cgattctggg aaagtgccga
      181 ttgttcacga caaagaccgc attaaagcgg gagatcgaat tacggaaggc ttaatcagcc
      241 ccaaggaatt gctccgtgtt ggaacggttg aagctgttga aaactatatc cttaaagaag
      301 tacaaaaagt ttatcgctct caaggtgtgg aaattgccga taagcatatt gaaataatca
      361 tcaaacaaat gatgcaaaaa gtagccatta ttcatgaggg ggggacggat ttgcttccag
      421 gcagttccat ttccaaagcc gaattatatg actatgttca agcttgtatt aaagaaggta
      481 aagatgttcc gattgcaaaa tcggttttac tgggaataac tagagcttcg ttaaaatcgg
      541 attccttctt gtcggcggcc agtttccaag aaacgacgag agtcttaacc gaagcagcca
      601 ttcggggaaa gcgtgactct ttagaaggct taaaggaaaa tgttatcatt ggtggtttaa
      661 ttccagccgg aacagggcta attgatgctg actttattga agtccctgat ttagaagagg
      721 aagaagagga gatttaaaaa tcgctcttga ctttaatttt aaaaagtgta taatatacta
      781 gtaaaaaaaa taaattgggt tttggcttgc caagacctaa tttaaggcaa atatgaaaca
      841 cccggtaatg tggtagtttt agaaaggaga agatatgccg actattaacc aattagtaag
      901 aaaaccaagg gaaagtaaaa aacgtaaatc caagtcccca catctaagtg ttagttttaa
      961 tactttaaag aagacttata ctgaagttaa atctccgcaa aaacggggcg tttgcacacg
     1021 tgttgctacg ttgactccta gaaaacccaa ttcggccatt cgtaagtatg cccgtgttcg
     1081 attatccaat ggcatcgaag taacggctta cattccgggc attggtcatc gcttacagga
     1141 acatagcgtc gtgttaatca gaggtggtcg tgttaaagac ttacctggag ttcgttatca
     1201 catcattcgt ggtactcaag atgctacggg agttgaaaaa cgtgttcaag gacgaagcaa
     1261 atacggtgca aaaaaaccta aatagaagaa tatttgaaag gaggaaataa ttatgccacg
     1321 taaaggacat ataccaaaaa gaaaagtatt accggatccc atttataatt cgccaattgt
     1381 tacccgatta attaatggca ttatgttaga tgggaaaaaa ggcgtcgctc aacaaattct
     1441 ttatggcgcc tttgaaaaaa ttgagaaaaa aacgggtcgc tccgctttag atgtttttaa
     1501 tgaagcttta aataatatca cgcctcaatt ggaggtgcgt gctcgccgaa tcggcggtgc
     1561 aaattatcaa gttccggtcg aagttcgccc ggaaagaaaa atgactttgt gtttaagatg
     1621 gttagttcaa tattctcgtt acggaaagaa aaaacgatgg aagaaagatt agccaatgag
     1681 attttagata ctgctaatgg cgtcggcaat acggttaaaa agcgcgacga tacccatcgg
     1741 atggctgaat ccaataaagc cttcgcacat tatcagtggt aattggagtt aagttatgcc
     1801 tcgtgaaatc tcattagaca aaacccgcaa cattggaatc atggctcaca ttgacgctgg
     1861 aaaaacgaca gttacggaac gcattctctt tcatactgga aaaatccaca aaatgggtga
     1921 gactcatgat ggtgcagcca ccatggattg gatggagcaa gaaatggaac gcgg
//
LOCUS       DAOSGN010000164         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_58511_length_1958_cov_3.452444, whole genome shotgun sequence.
ACCESSION   DAOSGN010000164 DAOSGN010000000
VERSION     DAOSGN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58511_length_1958_cov_3.452444"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK087_02815"
     CDS             complement(<1..568)
                     /locus_tag="PK087_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH18237.1"
                     /translation="MLISFSISNFMSIGSKQTLSMIPSLPTSPKKHMLRVDERELLKH
                     AVIFGANSSGKSNFLRAMAFGKQIVLAGLSPKFKFAYSKLGDDSQPRITTWEYTFFIN
                     HHFFSYGFSVNFNTLEIVEEWLLEIDPQTDHSHLLFERHVAEQSFYSPINNYHLAGSD
                     VKAKMLLVSDYHRHSQISVLATVYQWFAK"
     gene            complement(620..1768)
                     /locus_tag="PK087_02820"
     CDS             complement(620..1768)
                     /locus_tag="PK087_02820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH18238.1"
                     /translation="MNKLSRLETILTNNQLVGGGYLVLHKENDQFTIASHYERGLANR
                     ETGQALSLETIFRIASVSKMVVALAMMSFVEKKKVTIDSDIGDILGYRVRNPHFPHHP
                     ITIKMLMTQTSSLTDGYSDETPTLRGYNGVNGQHFAVKLADLLVPNTGPYYTDCTWDQ
                     REPGSTFIYSNFGCGILACLIEKLSNEPFVHYVKKTILDPLQIDASFQATTISRPDLI
                     ASLYYAKPQTGFVLARSQADFIKAVYPLFTLGDNFRGPAGGMFISLRGLARIAELFLN
                     RGVVQGKRLFQTETIDLMLAKHWQGANDEYYAKGLQIRFLPFERLLKGHTGSAYGLSS
                     LLFFNEETQTALCFASNGGYFYPAALGLNQVQEQLIRAYYEEFVQKKL"
BASE COUNT          611 a          409 c          382 g          556 t
ORIGIN      
        1 gcttggcaaa ccattggtaa acggttgcta aaacactgat ttgcgaatga cgatggtaat
       61 cacttaccag taacatcttc gcttttacat ccgaaccagc gagatgataa ttattgattg
      121 gcgaataaaa gctttgctcg gcaacatgac gctcaaataa taagtgactg tggtcggttt
      181 gcgggtcaat ttccaatagc cattcttcaa caatctctaa agtattaaaa ttaacactaa
      241 aaccatagct aaaaaagtgg tgattgataa aaaaggtata ctcccaagtg gtaatccgtg
      301 gttgggaatc atcgcccaat ttggaataag caaatttaaa tttaggagat aatcctgcta
      361 aaacaatttg ctttccgaag gccattgctc gcaaaaaatt actttttccc gatgaattag
      421 caccaaaaat aacggcatgt tttaaaagct ctcgctcatc aacccgaagc atatgttttt
      481 ttgggcttgt tggcaagctg ggaatcatgg acagtgtttg tttggagcca atggacataa
      541 aattgctaat ggaaaaactg attaacataa aaccctccta taatttgata aaagcgtcca
      601 agagtgatat tccccttgat taaagtttct tttgtacaaa ttcttcataa taagccctga
      661 ttaattgttc ttgcacttga tttaaaccta gagcagccgg atagaaatat cccccgttac
      721 tggcaaaaca aagggccgtc tgcgtttctt cgttaaagaa taataaacta gacaaaccat
      781 aagcggagcc ggtatgccct tttaaaagac gctcaaaagg aaggaaacga atttgtaggc
      841 ctttcgcata atattcatca ttcgccccct gccagtgttt agcaagcatt aaatcaatgg
      901 tttcggtttg gaaaaggcgc tttccctgaa cgacaccacg gtttaaaaac aattccgcaa
      961 tccgcgcaag tccccttaaa ctaatgaaca ttcccccggc cgggccccga aaattatcgc
     1021 ctaaggtaaa taaaggataa acagccttga taaaatccgc ttgcgagcga gccaagacaa
     1081 accctgtttg gggcttggca taataaagcg aggcaattaa atcgggccga gatatcgttg
     1141 tggcttgaaa gctcgcatca atttgtaagg gatcaagaat ggttttcttc acatagtgaa
     1201 caaagggctc attgcttaat ttttcaatta aacatgctaa aataccgcag ccaaaattgg
     1261 aataaataaa ggttgagccc ggttcacgtt gatcccaagt acaatcggta taatatggtc
     1321 ccgtattggg aactaataaa tcagctaatt tgaccgcaaa atgctgtcca ttaaccccat
     1381 tatagcctcg aagcgttggc gtttcatcgc tataaccatc ggttaagctg gaggtttggg
     1441 tcattaacat tttgatggta ataggatgat ggggaaaatg aggattgcga acccgatagc
     1501 ctaaaatatc accgatatcg ctgtcaatcg tcactttctt tttttccaca aatgacatca
     1561 tcgctaaggc gacgaccatt ttcgaaaccg atgcaatccg aaaaattgtc tcaagggaga
     1621 gcgcctgacc agtttcccga ttagcaagcc ccctttcgta atgactggcg atagtaaatt
     1681 ggtcgttttc tttgtggaga actaaatacc ccccgcctac taattgatta ttagttagga
     1741 ttgtttctaa acggctaagt ttgttcattt tttctcctct tcttctactc ctttagacta
     1801 aataagtttg cggatcatta agcatttctt gaatcttcaa aatccccaaa caataaggat
     1861 agccatcgat taaagcatgg tgagcttgaa tttgtaaagc aatcagggtt ttcccgatgg
     1921 ctatccaaat tcaagctcac catgctttaa tcgatggc
//
LOCUS       DAOSGN010000165         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_59351_length_1940_cov_2.550663, whole genome shotgun sequence.
ACCESSION   DAOSGN010000165 DAOSGN010000000
VERSION     DAOSGN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59351_length_1940_cov_2.550663"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PK087_02825"
     CDS             <1..410
                     /locus_tag="PK087_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOH18239.1"
                     /translation="ISLGITIKEDNTASVSGGLLVQLLPDALEEDIVYLESKTAVLKN
                     FSQLLKQYERLEDLLDYLFDHKVTILETLPVTYACHCSKEHFSRGIATLGAEEIDKII
                     HEDHQAEIICHYCKTPYLYNIDDLESIKKGLKK"
     gene            407..1417
                     /locus_tag="PK087_02830"
     CDS             407..1417
                     /locus_tag="PK087_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOH18240.1"
                     /translation="MNILAFAKQILEIPSPSGYTKNVINFLKKHCEKEGFQHHLTQKG
                     NLIIQFPGLDQYRLGLSAHVDTLGAMVKSIAADGTLKFSVIGGPLLPTYDGEYCYILT
                     RSGKQFTGTFLSNAPATHVYKEASTMPRTQDTMHIRLDAKVKNKSDVEKLGIGPGDFI
                     ALDPKTVITESGFIKSRFLDDKISVAILFALMDHIKKTKMVLKHSLSIIISTYEEVGH
                     GAASIPDLDEMIAVDMGCIGDDLSCTEYDVSICAKDSFSPYDFDITNNLVAIAKKEKL
                     SFALDIYPYYGSDVSAALRSGNNLKGGLVGPGVSASHGMERTHEDAVNNTLKLLIAYL
                     QA"
     gene            complement(1441..>1940)
                     /locus_tag="PK087_02835"
     CDS             complement(1441..>1940)
                     /locus_tag="PK087_02835"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOH18241.1"
                     /translation="IVDESTVAEVVKEYQNPQDYVMQMAILKWEDVFSRHPKDLVICA
                     DTIVCLDELILGKPHNPLEAQAMLQTLSGRTHNVLTGVYLRLGAKKKVFVVKTEVSID
                     KLSEEEINQYIMMGEAYDKAGAYAIQGFFGKYVKKIRGDYYNVMGLPLNRLYRELQKF
                     NQKTS"
BASE COUNT          582 a          410 c          372 g          576 t
ORIGIN      
        1 taatcagttt gggaattacc atcaaagaag acaatacggc ttctgtcagt ggcggtttgt
       61 tggtccaatt attacccgac gccttggaag aagatatcgt ttacttggaa agtaaaaccg
      121 cagttttaaa aaacttttcc caattactta agcaatacga acgcttggaa gatttgcttg
      181 attatctgtt tgatcataag gtcaccattt tggaaaccct ccctgtcact tatgcctgcc
      241 attgttccaa agagcatttt tccaggggca tcgctacttt gggggctgaa gaaatcgata
      301 agattatcca cgaagaccat caagcagaaa tcatctgcca ttattgtaaa actccttatc
      361 tttataacat agatgacttg gaaagcatta agaaaggatt aaaaaaatga acattttagc
      421 ttttgcaaaa caaatcttag aaatccccag ccccagcggc tataccaaaa acgttattaa
      481 ctttttaaag aaacattgtg aaaaggaagg cttccagcac catcttacgc aaaaaggcaa
      541 tctcattatc caatttccgg gccttgatca atatcgcctt ggcctcagtg cccatgttga
      601 cactttagga gcgatggtca aatcgattgc tgccgacggc actttaaagt tttcggttat
      661 tggcggacca ttgcttccga cttacgacgg tgagtattgt tatattctaa cgagaagcgg
      721 caagcagttt acgggaacct ttctatccaa tgcgccggcg acccatgttt acaaagaagc
      781 ttcaaccatg ccccgcactc aggacacgat gcatattcgc ctagatgcca aagtgaaaaa
      841 caaaagcgat gtggaaaaac tgggcattgg gcccggtgat tttatcgccc ttgacccgaa
      901 aaccgtgatt acggaatcag ggttcattaa atcccgtttt ttagatgaca aaatcagtgt
      961 cgccattttg tttgctttaa tggaccacat taaaaaaacc aagatggttt taaaacattc
     1021 tctaagtatc attatctcga cctatgaaga agttggtcac ggtgctgctt ccatccctga
     1081 tttggatgaa atgattgctg ttgatatggg atgtatcggt gatgatttaa gttgtacgga
     1141 atatgatgtt tccatttgcg ccaaggactc gttcagcccc tatgatttcg atatcaccaa
     1201 caatctagtg gcgattgcta aaaaagaaaa attatccttt gctttagata tttatccgta
     1261 ttacggctcc gacgtttcag ccgctttaag aagcggcaac aacctcaaag gcggtcttgt
     1321 gggtccgggt gtgagtgctt cccacggcat ggaacgaact catgaagatg ccgtcaacaa
     1381 cactttgaaa ttactgattg cttatttaca agcctaatga aaaacctctt aaagacaaat
     1441 ttaagaggtt ttttgattga acttttgtaa ttcacgatat aaacggttta aaggaagacc
     1501 catgacatta taataatcgc ccctgatttt ctttacatat tttccaaaga aaccttgaat
     1561 ggcataagca ccggctttgt cataggcttc gcccatcata atgtattgat taatttcttc
     1621 ttctgacagt ttatcaatcg aaacttcggt tttcacaaca aacactttct ttttagcacc
     1681 gaggcgtaga tatactccgg tcaaaacatt atgggttcga ccggataaag tttgtaacat
     1741 cgcttgggct tctagcggat tatggggttt tcccaagatt aattcatcca aacaaacaat
     1801 cgtatcggca caaataacca aatcttttgg atgtctggaa aaaacatcct cccattttaa
     1861 gatggccatt tgcatcacat aatcttgcgg attttgatat tctttcacaa cttctgcgac
     1921 tgtcgattca tcaacaataa
//
LOCUS       DAOSGN010000166         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_59869_length_1929_cov_2.664354, whole genome shotgun sequence.
ACCESSION   DAOSGN010000166 DAOSGN010000000
VERSION     DAOSGN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59869_length_1929_cov_2.664354"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PK087_02840"
     CDS             complement(<1..608)
                     /locus_tag="PK087_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH18242.1"
                     /translation="MAYNHQEIEKKWQKYWETHKTFKTGQDLSKPKYYVLDMFPYPSG
                     AGLHVGHPEGYTATDAIARMKRMQGYNVMHPMGWDAFGLPSEQYAIATGKDPFDFTMQ
                     NIKTFKHQLGILGLSYDWDRELATCDPKYYKWTQWIFTILYKKGLAEIRNIDVNWCDE
                     LGTVLANEEVINVDGKMVSERGCYDVVKKPMRQWVLKITKYA"
     gene            complement(613..1422)
                     /locus_tag="PK087_02845"
     CDS             complement(613..1422)
                     /locus_tag="PK087_02845"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HOH18243.1"
                     /translation="MYQRFKEGLFSPSEIAKYQNDKKIVTFLFFLILVLLSIIPSLIN
                     LTHNRGLDYEERTTIRQSFLDNEVHFSLVDSQLVADVGTNKIQTVLVNSTLLIVFTDQ
                     DSLKTDLSHFNTYSRIVLTKKAVYFQQSLINVKLFAYADYPSLANLDFSDALKNDQNF
                     WTTVFLVIEDQIAHHGSVTLLAKGLFIVISEILVLGFFSLIVAIFQMMSLSGYMKFTK
                     VWQIMTYILVPYIVAKVFEDLFSLNYLSFLGAIITVFYANRLTQVILQRKD"
     gene            complement(1501..>1929)
                     /locus_tag="PK087_02850"
     CDS             complement(1501..>1929)
                     /locus_tag="PK087_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formiminotransferase"
                     /protein_id="HOH18244.1"
                     /translation="PLVAFNIDMNSNDKGKVLKIARRIRYSSGGYRFIQAGAARLQER
                     RLCQVTMNLTDYEQTPLYQALEATMMEARRYQMDVVASEIVGLVPKACLVNSLKYYLH
                     LEDDAVLDLSLPDIVELSMKYFKLRDFNEQKIIEYYVKNK"
BASE COUNT          635 a          384 c          316 g          594 t
ORIGIN      
        1 tcagcatatt tagtgatttt taagacccat tggcgcatcg gttttttcac aacatcataa
       61 cagccgcgtt cactcaccat tttgccatca acattgataa cttcttcatt ggcgagtacc
      121 gtccctaatt catcgcacca attaacatcg atattgcgaa tttccgctag gcctttttta
      181 taaaggattg taaaaatcca ttgcgtccat ttatagtatt tcggatcaca agtggccaat
      241 tcccgatccc aatcatagga caaacctaaa attcccaact gatgtttaaa tgttttaatg
      301 ttttgcattg taaaatcaaa gggatctttg ccggtagcaa ttgcatattg ttcagagggc
      361 agtccaaagg catcccagcc cattggatgc ataacattat agccttgcat gcgtttcatg
      421 cgcgcgatgg catcggtggc cgtgtaaccc tcgggatgcc caacatgcaa gcctgcccca
      481 cttgggtaag gaaacatatc taaaacataa tatttcggtt tggacaaatc ttggcccgtt
      541 ttaaaagttt tatgggtttc ccaatatttt tgccattttt tctcgatttc ttgatgattg
      601 taagccattt ttttaatcct tcctttgcag tattacttgt gttaaacgat tagcatagaa
      661 aaccgtgatg atggctccta aaaaagataa gtaattaagg gaaaataaat cttcaaatac
      721 tttcgcaaca atatacggaa cgagaatata ggtcataatt tgccaaacct tggtaaattt
      781 catatatccc gatagtgaca tcatttgaaa aatagccacg attaagctaa aaaatcctaa
      841 taccaatatt tcactaatga cgataaaaag cccttttgcc aataatgtaa ctgacccatg
      901 atgagcaatt tgatcttcaa tcacgaggaa gacggtcgtc caaaagtttt gatcattttt
      961 taaagcatca ctaaaatcca aattggctaa tgagggataa tcagcataag caaacaactt
     1021 gacattgatt aatgattgtt gaaaataaac ggccttcttc gttaatacaa tccgtgaata
     1081 agtattaaaa tggcttaaat cggttttcaa gctgtcttga tcagtaaaca caatcaacaa
     1141 tgtggaattt accaaaaccg tttggatttt gttagtcccg acatccgcca ccaattggga
     1201 atcaactaaa gaaaagtgaa cttcattgtc caaaaaggat tgacgaatag tcgttcgttc
     1261 ttcataatcc aagccacgat tgtgagtcaa attaattaaa ctcgggataa tagaaagcaa
     1321 aacgagtatt aaaaaaaaca aaaaagttac tattttttta tcgttttggt atttcgcaat
     1381 ctctgaagga ctaaatagtc cttctttaaa tctttgatac atggaggtct ccttgatatt
     1441 gactaattat aacaaaatca atcctgtttt tcaaattaaa aaccaaaaaa cctgacatta
     1501 ttacttattc tttacataat attcgattat tttttgttcg ttaaaatcac gcagtttaaa
     1561 atacttcatt gatagttcaa cgatgtctgg aagagacaag tctaaaaccg catcatcttc
     1621 taaatgaaga taatatttca atgaattaac caaacaagct ttgggaacta gcccgacgat
     1681 ttcgctagca acaacatcca tttgataacg gcgagcttcc atcatcgttg cttccaaagc
     1741 ttgatagaga ggcgtttgct cataatccgt taaattcatt gttacttgac acaaacgacg
     1801 ctcctgtaaa cgagcggcac cggcttgaat gaatcgataa ccacccgaac tataacgaat
     1861 gcgacgggca atttttaaga ccttcccttt gtcattactg ttcatatcaa tattaaaagc
     1921 cactaaagg
//
LOCUS       DAOSGN010000167         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_59908_length_1928_cov_2.739455, whole genome shotgun sequence.
ACCESSION   DAOSGN010000167 DAOSGN010000000
VERSION     DAOSGN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59908_length_1928_cov_2.739455"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /gene="rsgA"
                     /locus_tag="PK087_02855"
     CDS             complement(<1..584)
                     /gene="rsgA"
                     /locus_tag="PK087_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOH18245.1"
                     /translation="MQGLVIKSLSGDFTVFSNHQNYICKPKGLFKYQEKLVKVGDKVV
                     FDPETLIIDEVLPRKNDLIRPAIANIDKIFCVFSVKEPELNLNLLDRLLSIFTYHNLH
                     CLIIFTKLDLLTDYQDIDVLIQYYQKIGYAVYTSASALDLEAIKKEISQSICAFAGQS
                     GVGKSTLLNLFDESLNIKTDVISKALGRGKHTTR"
     gene            complement(572..1612)
                     /gene="rlmN"
                     /locus_tag="PK087_02860"
     CDS             complement(572..1612)
                     /gene="rlmN"
                     /locus_tag="PK087_02860"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009488526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOH18246.1"
                     /translation="MQTSIYNYAKDQLEQYFLSIDSPKFRATQVFEAVYKQRLTDFTK
                     MSNFKKEFVGKLQDDFYFSQLKVVKVQSSEDGTKKILYQLDDGNLIETVLMKNNYGYS
                     VCVTTQVGCNMGCAFCASGLTKRVRNLETAEMVLQVLTIDNCLREQNERVSHVVIMGI
                     GEPFDNYDNVLSFIKIINDGKGLEIGSRHITLSTCGIIPKIYQFSEFPLQVNLAVSLH
                     FATDEKRSQYMKINRSYNLTELKKALQYYFAKTKRRITFEYILLDGINDSLEDAKLLL
                     DYVRDMNAYINLIPYNETGKQFKRSKDGRSRSFFDFLMKNKLQVTLRREQGHDIDAAC
                     GQLRVKELLCKD"
     gene            complement(1614..>1928)
                     /locus_tag="PK087_02865"
     CDS             complement(1614..>1928)
                     /locus_tag="PK087_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOH18247.1"
                     /translation="VLADVPCSGLGVLSHKVDIKYHLSLNSIQEIKKLQAAILEKTWG
                     IVKKGGFYTYSTCTINKEENEEQIRTFLSNHPEFKIVHEETILPHEYQCDGFYICKMR
                     RG"
BASE COUNT          628 a          376 c          299 g          625 t
ORIGIN      
        1 tgtcgagtgg tatgcttccc tcgacctaga gctttagaaa taacatccgt tttaatattc
       61 aagctttcat caaaaaggtt gagcaaggtg cttttgccta ccccgctttg gccggcaaag
      121 gcacaaatgg attgactaat ttcctttttg atagcctcta agtccaatgc actggcggaa
      181 gtataaaccg cataaccaat cttttgataa tactgaataa gcacatcaat atcttgataa
      241 tcagttaata aatctaattt ggtgaagata ataagacaat gaagattatg ataagtgaag
      301 attgatagca ggcgatccaa caaattaaga tttaattccg gttctttaac ggaaaaaacg
      361 caaaagattt tatcaatatt agcgattgcc ggccgaatta aatcgttttt gcgagggaga
      421 acttcatcga ttatcaaagt ttcaggatca aaaacaactt tatcgccaac tttcactagt
      481 ttttcttgat atttaaataa acccttaggt ttacaaatat aattttgatg gttagaaaaa
      541 acggtgaaat caccacttag tgatttgatg actaatcctt gcataagagt tcctttacac
      601 gtaattgacc acaagccgca tcaatatcat ggccctgttc tcttcttaag gtcacttgga
      661 gtttattttt cattaaaaaa tcaaaaaagg aacgggagcg accatctttt gaccgtttaa
      721 actgcttccc ggtttcatta taagggatta aattaatata agcattcata tccctcacat
      781 aatcaagcag taatttggca tcttctaaac tatcattaat cccatcaagt aaaatatatt
      841 caaaagtaat ccgccgtttg gtcttggcaa agtagtattg caaggccttt tttaattccg
      901 ttaaattata actacgatta atcttcatat actggctgcg tttttcatcg gtagcaaaat
      961 gcaaagacac tgctaaatta acttgcaacg ggaattcgct aaattgatag attttcggaa
     1021 taataccgca agttgataag gtaatatggc gactaccaat ttctaaaccc tttccatcat
     1081 taatgatttt aataaaacta agcacattat catagttatc aaagggttcg ccgatgccca
     1141 taatcaccac atggctaaca cgttcatttt gctcacgcaa gcaattgtca atcgttaaaa
     1201 cttgcaacac catttccgct gtttctaaat tacgcactcg tttagttaaa ccgctggcgc
     1261 aaaaagcaca acccatgttg cagccaactt gggtggtcac acacacactg tagccataat
     1321 tatttttcat taaaaccgtt tcaattaaat tgccatcatc aagctgatat agaattttct
     1381 ttgttccatc ttcagagctt tggactttca caactttcaa ttgggaaaaa taaaaatcat
     1441 cctgtaattt gccaacaaat tcttttttaa aattgctcat cttagtaaaa tccgtcagcc
     1501 tttgtttata aacggcttca aaaacctgag tagcccgaaa tttagggcta tcaatactta
     1561 gaaaatattg ttctaattgg tctttggcat aattatagat tgaagtttgc aaattaacct
     1621 ctcctcattt tacatatata gaaaccgtca cattgatatt cgtgtggtaa aattgtttct
     1681 tcatgaacaa tcttaaactc agggtgatta cttaaaaagg tcctaatttg ttcctcgttc
     1741 tcctctttat tgattgtaca agtggaataa gtataaaaac cacccttttt cacaatcccc
     1801 caggtttttt ctaaaatggc agcttgcagc ttttttattt cttggatact gtttaaagac
     1861 aaatgatatt taatgtcaac cttatgtgat aataccccta agccggaaca aggaacatcc
     1921 gctaaaac
//
LOCUS       DAOSGN010000168         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_60253_length_1921_cov_2.781886, whole genome shotgun sequence.
ACCESSION   DAOSGN010000168 DAOSGN010000000
VERSION     DAOSGN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60253_length_1921_cov_2.781886"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /gene="rimI"
                     /locus_tag="PK087_02870"
     CDS             <1..323
                     /gene="rimI"
                     /locus_tag="PK087_02870"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOH18248.1"
                     /translation="YLVLEIDQQVGGYIGLWIHDDKAEIINFYVDKAYQHQGFGTMLL
                     DFACQLCELSGVSMLSLEVRENNNAALALYEKFNFVFSHKRHQYYQDLSDALVLIKKF
                     EEAK"
     gene            320..1273
                     /gene="tsaD"
                     /locus_tag="PK087_02875"
     CDS             320..1273
                     /gene="tsaD"
                     /locus_tag="PK087_02875"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOH18249.1"
                     /translation="MIVLGIETSCDETSVALVKDGREVLSNVVYSQIDVHKVYGGVVP
                     EVASRHHVQKITVVFEEALKEAQISPQDIDLVAVTTHPGLIGSLLVGINAAKAFALAH
                     GIKLVEVNHLHGHIYANYLESDFYFPVLALVVSGGHTELILMKDHFQFEVLGETLDDA
                     VGESYDKVGRVLGLEYPAGMKVDALAITGTPSYQLPLVYLGKANFNFSFSGLKSAVIN
                     LINTAHMKKETIDIANLCASFQKVVVQVLVDKTMQAAIMHQVKHIVVAGGVAANKGLR
                     QEMQAAVAKHPPLKLSFPPLKYCTDNAAMIAVAGYFQDKGE"
     gene            1375..>1921
                     /locus_tag="PK087_02880"
     CDS             1375..>1921
                     /locus_tag="PK087_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18250.1"
                     /translation="MKKFSIAIIMLLILVLAGCGDSSSPERLWNHYVSAMNAKDIEAV
                     AGSFYLEDSADYHKFIENNNPEVYFADFTSIKTRSFKPEVVNENFYCADITISLKDAS
                     GSQSKQFLVYFIRDLNTGWKFTNEVNVTAYDMALLGNEPSAYYYANVVKNAEGFDYKY
                     IYAGIAGEAGDDDFVKIVAPNS"
BASE COUNT          561 a          336 c          439 g          585 t
ORIGIN      
        1 attacttggt tttagagatt gatcagcagg tgggtggcta catcggttta tggattcacg
       61 acgataaggc cgaaatcatt aacttctatg ttgataaagc ctatcaacac caggggtttg
      121 gaacgatgct gctggacttt gcttgccaat tatgcgagtt aagcggggtg tcaatgctta
      181 gtttagaagt gcgggaaaat aataatgccg ctttagcttt atatgaaaaa tttaactttg
      241 tgttttctca caaacgtcat caatactatc aggatttaag cgatgcttta gtgctgatta
      301 aaaaatttga ggaagcaaaa tgattgtttt aggaatagaa accagttgtg atgaaaccag
      361 tgtcgcgctt gtgaaagacg gtcgcgaggt tttgtccaat gttgtttata gtcaaattga
      421 tgttcataaa gtttatggcg gagtagttcc ggaagtggcg agtcgccacc atgtccaaaa
      481 aataaccgtc gtttttgaag aagctttaaa agaagcccag attagccctc aagacattga
      541 tttggtagcc gttaccaccc atcccggttt aatcggttcg cttttggtcg gcatcaatgc
      601 ggccaaagct tttgctttag cgcatgggat taaactagtt gaagtcaatc acctccatgg
      661 tcatatttat gccaattact tggaaagtga tttttacttt ccggttttag ccttggtggt
      721 ctccggtggg catacggaat taatccttat gaaggaccat tttcagtttg aagtgctcgg
      781 cgaaaccttg gatgatgctg ttggagaaag ttatgataaa gttggtcgag tgctgggttt
      841 ggaatacccg gccggaatga aggtggacgc tttagcaata acaggcactc ccagctacca
      901 actgccctta gtttatttgg gtaaagccaa tttcaatttc tccttttccg gattaaaatc
      961 cgctgttatt aatctcatta atacggctca tatgaaaaaa gaaacgattg atatcgccaa
     1021 tctttgtgcc tctttccaaa aggttgtggt gcaagtgtta gttgataaaa ccatgcaagc
     1081 ggccatcatg catcaagtga agcatattgt tgtggcgggg ggagtagccg caaataaagg
     1141 tcttcgccaa gagatgcaag ccgcagtggc gaaacatccc cccctaaaat taagctttcc
     1201 gccccttaaa tattgtaccg ataatgcggc tatgattgca gtggccggtt attttcaaga
     1261 taaaggcgaa taattataaa aaaagtttga tttacaaggt aaattattat ataatcaaat
     1321 aagtaaactg tggaaaaacc ataaccaagc atttattgat ggaggccaat gcgagtgaag
     1381 aaattttcaa ttgccataat catgttatta attttagtgt tggcgggatg cggcgacagt
     1441 agttctcccg agcgactttg gaatcattat gtcagcgcca tgaacgccaa agatattgaa
     1501 gcggtggccg gtagttttta tttggaagac tccgctgatt atcataaatt tattgaaaac
     1561 aataatccgg aagtttattt tgcggacttt acttctataa aaacccgctc ctttaaaccg
     1621 gaagttgtga atgagaattt ttattgtgca gacattacga ttagcttaaa ggatgccagt
     1681 ggtagtcaaa gcaagcagtt tttagtttac tttatcaggg atttaaatac aggatggaaa
     1741 tttaccaatg aagtcaatgt cactgcttat gatatggctt tacttggcaa tgaacccagc
     1801 gcttactatt atgcgaatgt tgtgaaaaac gcagagggct ttgattataa atatatttat
     1861 gccggtattg ccggtgaagc cggtgatgat gattttgtga aaatcgttgc tccaaacagt
     1921 a
//
LOCUS       DAOSGN010000169         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_60473_length_1916_cov_3.315959, whole genome shotgun sequence.
ACCESSION   DAOSGN010000169 DAOSGN010000000
VERSION     DAOSGN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60473_length_1916_cov_3.315959"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..>1916
                     /gene="ligA"
                     /locus_tag="PK087_02885"
     CDS             52..>1916
                     /gene="ligA"
                     /locus_tag="PK087_02885"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOH18251.1"
                     /translation="MDPKQRIIELVNLLNQYSYEYYVDDNPSVTDLEYDKLLRELEQL
                     EKENPQYILKNSPTQKVGAKAAPKFEKIRFVKPMLSLANAFTKAEIEEFHERIIKEGF
                     HPSYVCELKIDGIASSAVYEKGQFVQGATRGDGEEGENITANMLTIKSLPKTINELNA
                     LEVRGEVYMKKSVMDQLNSERETEGESLFKNPRNAAGGSLRQLDSSITAQRKLDLFVY
                     TVVNPEKYHLSTQMEAMAFLIKQGFTVNPHYRLCQSIEEVWDYLQSWKDKRRELDYET
                     DGVVIKVNDFTLQEEIGYTIKNPKWALAYKFPPLEVETKLLEIVFSVGRTGGITPIAI
                     LEPVLLAGSTVSRATLNNEDFIKERDIEIGDYVVIRKAAEIIPEVIKVNLEKRQNTKP
                     FVMATNCPQCGSTLVRAEGEADHYCLNEQCPGRNKASLIYFASKVGMNIDGLGEKLVE
                     ELMNRGFIKTITDLYRLENHREALMNLPGMGEKSVNNLLIAINNSKNNPLAKVITALG
                     IRLVGTKAAKELVKYYPNLSALKQARLEELMNIKDVGPATANSLVGFMKQNSSLIDEL
                     ISLGINPQETKTAKQSDILKGMTVVLTGKLETMTREEASLLVEKHGGQTAGSVSK"
BASE COUNT          634 a          333 c          439 g          510 t
ORIGIN      
        1 cgcggagttg tggtataata tttcattgta agaggacttg tgagggaaaa tatggatcca
       61 aagcaacgca ttatcgagtt agttaatttg ttgaatcaat atagttatga atactatgtc
      121 gatgataatc ccagcgtcac tgatcttgaa tatgataagt tactaagaga attagaacaa
      181 ctggaaaaag aaaatcccca gtatatttta aaaaactcgc ctactcaaaa agtaggagcg
      241 aaagccgctc ctaaatttga gaagattcgc tttgttaaac cgatgttatc cctagccaat
      301 gccttcacca aagcggaaat tgaagagttc catgaacgaa ttattaaaga gggctttcat
      361 cctagttatg tctgtgaatt aaagattgat gggattgcca gttccgctgt ttatgaaaag
      421 ggacagtttg ttcaaggagc aacccgcggt gacggtgagg aaggcgaaaa catcaccgcc
      481 aatatgttaa cgattaagtc tttgccgaag acaattaatg aattgaatgc tttagaagtc
      541 cgtggcgaag tttatatgaa aaagtccgtt atggatcagt taaacagcga acgggaaact
      601 gaaggggaaa gtttgtttaa aaatcccagg aatgctgccg gaggttcttt aagacaacta
      661 gacagtagta ttaccgccca aagaaaactt gatttatttg tttatacggt tgttaatccg
      721 gaaaaatatc atttaagcac tcaaatggaa gcgatggcat tcttaattaa acagggcttt
      781 acggttaatc cgcattatcg cttgtgtcaa agcattgaag aagtttggga ttacttgcaa
      841 tcttggaagg acaaacgccg ggaactggat tatgaaaccg acggtgttgt catcaaagtc
      901 aatgatttta ctttacaaga agaaatcggt tatacgatta aaaacccgaa atgggccctt
      961 gcttataagt ttccgccttt agaagtggaa acgaaattgt tggagattgt tttttcggtt
     1021 ggtcgaaccg gcgggattac gccgatagcc attttagaac cggttttatt agcaggttca
     1081 accgtttcaa gggccaccct aaacaatgaa gatttcatta aagagcgtga tattgaaatc
     1141 ggcgattatg tggtgattcg caaagcggcc gaaatcattc ccgaagtaat taaagtgaat
     1201 ttggaaaaac gccaaaatac caagcctttt gtgatggcga ccaactgccc ccagtgtggc
     1261 agcaccttgg tgcgggcaga aggtgaagcc gaccactatt gtttgaatga acagtgtccg
     1321 ggaagaaata aggccagcct tatttatttt gcttctaaag tggggatgaa tattgatggt
     1381 ttaggcgaaa aattggtcga agaattaatg aaccgaggtt ttattaaaac gattaccgat
     1441 ctttatcgct tagaaaacca tcgggaagct ttgatgaatt taccgggaat gggcgaaaaa
     1501 agcgttaata atttgcttat tgccattaac aactcaaaaa acaatccctt agcgaaagtt
     1561 attaccgcct taggaattcg tttggtggga acgaaagccg ccaaagaatt agtcaaatac
     1621 tatcctaatt taagcgcttt aaagcaagca agattggaag aactgatgaa catcaaagat
     1681 gtcggaccgg ccaccgctaa tagtttagtt ggttttatga agcaaaacag ttccttaatc
     1741 gatgaactca tcagtttagg cattaacccg caagaaacaa aaaccgccaa gcaaagtgat
     1801 atcctaaaag ggatgacggt ggttttaacc ggaaaactgg aaacgatgac aagagaagag
     1861 gcttccttac ttgtagaaaa gcatggcggt caaaccgccg ggtcggtgag taaaaa
//
LOCUS       DAOSGN010000170         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_60665_length_1912_cov_3.330641, whole genome shotgun sequence.
ACCESSION   DAOSGN010000170 DAOSGN010000000
VERSION     DAOSGN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60665_length_1912_cov_3.330641"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PK087_02890"
     CDS             complement(<1..791)
                     /locus_tag="PK087_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOH18252.1"
                     /translation="MFERLELMLSQYRKLNEDLQNPDIVANVKKVTELMKQMRALEKV
                     VSTYLRYQEITKTIADLKELSNSEDAELAEMAALEKEEALVEQEQLVQDLKLMLIPKD
                     PNDEKNVIVEIRGAAGGDEANIFAGDLFRMYSRYALERGWKIEVLQAVESEMGGFAQI
                     EFMVHGDNVYSFLKYESGSHRVQRVPETEASGRIHTSTATVLVMPEADDVDVELNLDE
                     VRIDIFCSSGPGGQSVNTTKSAVRVTHIPTGLVASCQDEKSQHEN"
     gene            complement(802..>1912)
                     /gene="dnaB"
                     /locus_tag="PK087_02895"
     CDS             complement(802..>1912)
                     /gene="dnaB"
                     /locus_tag="PK087_02895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOH18253.1"
                     /translation="ATGGTQYLFELIDNIPSIVNIAAYIEVIKEKSVERELLKKMQEI
                     ANRILDHKQPFAELIAEAEKDFIEVINKRKTVDLIRIDRATDKVLEIVEANKMKNTEG
                     LTGLDTGFKALNRLTFGFQKGELTILAARPGIGKSALALNFATNMCESMDAHVAFFSL
                     EMGIDQLLMRVYASHSGLTLNKIRAGKLSESEMAALYAAKTKIDKFHIYLDEAGATDI
                     EELVTVCRKLKRDNKLDFVVVDYLQLLTTKRHTTNRVDEVSKISRQLKMMARELQVPV
                     LALSQMSRFIERRDDKRPVLSDLRESGSIEQDADVVMFLHREPTKDDEDTTKRYRNAK
                     TELIIAKNRQGVTDSIFLIFKGAHSMFNPMDEVKD"
BASE COUNT          525 a          464 c          349 g          574 t
ORIGIN      
        1 ttattctcat gttgggactt ttcatcttga caggaagcga ctaaaccggt ggggatatgg
       61 gtgacccgca ctgccgattt cgtcgtattg acgctttgac caccgggacc ggacgaacaa
      121 aagatatcaa tccgcacttc atcaagattt aattcgacat ccacatcatc ggcttccggc
      181 atcactaaaa ccgtggctgt tgaggtatgg atgcgaccgc tagcttccgt ttccggaacc
      241 cgctggacac ggtgtgatcc cgactcatat tttaaaaagg aatacacatt gtcaccatga
      301 accataaatt caatttgggc aaaaccgccc atttccgatt caacggcttg taaaacctca
      361 atcttccacc cgcgttccaa agcgtaacgg gaatacatcc ggaataaatc accggcaaaa
      421 atattggctt catcgccgcc ggcagcgcct ctgatttcca caatcacatt tttttcatca
      481 ttggggtctt tcggaatcaa cataagtttt aaatcctgaa ctaattgttc ttgctcaaca
      541 agggcttctt ctttttccaa agccgccatt tccgctaatt cagcatcttc actattactt
      601 aattctttta aatcagcaat ggttttagta atttcttgat accttaaata agtcgaaacg
      661 actttttcca aagcccgcat ttgcttcatt aactcggtaa cttttttaac attagcaaca
      721 atgtcggggt tttgcaaatc ctcattcagt tttcgatact gactaagcat cagttctagt
      781 ctttcaaaca aaatatcact cttaatcttt gacttcgtcc atcgggttaa acatcgaatg
      841 ggcgcccttg aaaatcaaga aaatgctatc cgtaacacct tgacggtttt tcgcaataat
      901 caactccgtt ttcgcattgc gatacctttt cgtagtatct tcatcatcct tcgtcggttc
      961 acgatgcaaa aacatgacga catcggcatc ttgttcaata ctgcctgatt cccgcaaatc
     1021 cgataacacc ggacgtttgt catcacggcg ttcaataaaa cgggacatct gtgacaaggc
     1081 caaaaccgga acttgcagtt cacgggccat cattttcaat tgccgagaaa ttttcgaaac
     1141 ttcatcaacg cggttggtgg tatggcgctt cgtcgttaaa agctgtaaat agtcaacaac
     1201 cacaaaatcc agtttattat cccttttcag tttgcgacag acggttacta attcttcaat
     1261 gtccgtggcg ccggcttcat caaggtaaat gtggaatttg tcaattttgg ttttagcggc
     1321 atataaagct gccatttcgc tttcacttag tttcccggcc cgaattttat ttaaagtcaa
     1381 accggaatgc gaagcataaa cccgcattag caattggtca atccccattt ccaaggaaaa
     1441 aaaggcgaca tgggcatcca ttgattcaca catattggta gcgaaattaa gggcaagcgc
     1501 acttttacca atcccgggac gagccgccaa aatcgttaat tcgccctttt gaaaaccaaa
     1561 ggttaagcgg tttaaagctt taaagccggt atccagtccg gttaagccct cggtgttctt
     1621 cattttgtta gcttcaacaa tttctaaaac tttatccgtg gcccggtcaa tacgaattaa
     1681 atcgacggtt ttccgcttat tgatcacttc aataaagtct ttttccgctt ccgcaatcaa
     1741 ttccgcaaac ggttgtttat ggtccaaaat ccggttggca atttcctgca tttttttcag
     1801 taattcccgc tccaccgatt tttctttaat aacttcaata taagcggcaa tattgacaat
     1861 cgagggaata ttgtcaatca gttcaaataa atactgagtg cctccggttg ct
//
LOCUS       DAOSGN010000171         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_60892_length_1907_cov_2.962743, whole genome shotgun sequence.
ACCESSION   DAOSGN010000171 DAOSGN010000000
VERSION     DAOSGN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60892_length_1907_cov_2.962743"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PK087_02900"
     CDS             <1..309
                     /locus_tag="PK087_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOH18254.1"
                     /translation="FITKRLQKKFKVTIEEASALVNTLDSIKGYLVWIFFVQGKEKIR
                     VRLRSRFVDVNELAGEYGGGGHRQASGATLSHRRQIKEMIAKADLLVKQYKKDHPEVF
                     "
     gene            309..1037
                     /gene="surE"
                     /locus_tag="PK087_02905"
     CDS             309..1037
                     /gene="surE"
                     /locus_tag="PK087_02905"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOH18255.1"
                     /translation="MRILITNDDSIYSEGLWILAEAAKPYGEVVVVAPKNEQSAKSHA
                     INVRSGFPIEVFEGRFGVLSYTVDSSPADAVRAAYYALNLDFDLVLSGVNSGFNVGED
                     ILYSGTVAAATEAVLLGKKAIAFSVAPQDFATARTHVPTVLADIFQNKLLNEGDLFNV
                     NIPKNPQAMKITKQGKTYFDTRFEEEQGLYYQRGVHRYHLEEDCESDTWAIAHHLTSL
                     SPLTIKRIDFQVLQKILEKTTTKA"
     gene            1061..1609
                     /locus_tag="PK087_02910"
     CDS             1061..1609
                     /locus_tag="PK087_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18256.1"
                     /translation="MKTNDSLIELALRKMRKPKRARTIEDVAKDVFKEKSYPFKKDND
                     LFVQFNMDFMLSGHFICCDEDKNGVKLWDLKNRQPSSLLDKEGSYLDDLYKDDEEVLK
                     NELTDEVAFNQGTVKEFEAENYDEDDDEDNEEEHDDIEEELLNEGLTTDDEETITEKV
                     VLDEDDLDDEDDDDDELDFAHD"
     gene            1711..>1907
                     /locus_tag="PK087_02915"
     CDS             1711..>1907
                     /locus_tag="PK087_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOH18257.1"
                     /translation="MSTKFIFVTGGVSSSLGKGINASSIGRLLKQRGLKVSMQKFDPY
                     INVDTKNMSPLQHGEVFVTDDG"
BASE COUNT          617 a          339 c          437 g          514 t
ORIGIN      
        1 tttattacta aacgattaca aaagaaattt aaagtgacaa ttgaagaagc ttcggcactc
       61 gttaatacgc tggacagtat caaaggttat ttggtgtgga ttttcttcgt tcaggggaaa
      121 gaaaaaatcc gtgtgcggtt gcggtcgcgc tttgttgatg tcaacgaact ggccggggaa
      181 tatggtggtg gtggtcaccg ccaagccagt ggggccacgc tttcgcatcg gcgccagatt
      241 aaggaaatga ttgcgaaagc ggatttgctc gttaaacaat ataaaaaaga ccatcccgag
      301 gtgttttaat gcggatttta attactaatg atgattctat ttattcagaa ggactatgga
      361 ttttagcgga ggccgccaaa ccatatggtg aagtagtggt tgttgccccg aaaaatgagc
      421 aaagtgccaa aagtcatgcc atcaatgttc gttccggctt tcccattgaa gtttttgagg
      481 gcaggttcgg ggttttaagt tataccgtcg actcaagccc tgctgatgcc gtccgggccg
      541 cttactatgc tttaaatctg gattttgatt tagtgctgtc cggcgtaaac agcggcttta
      601 atgttggcga agatatccta tattccggaa cggtagcagc ggccacggaa gccgtccttt
      661 taggaaaaaa agcaatcgct ttttccgtgg ctccgcagga ctttgcaacg gcccggacgc
      721 atgtgcccac ggtcctagcc gatattttcc aaaacaaatt attaaatgag ggagatttgt
      781 ttaatgtcaa tattcctaaa aacccgcaag cgatgaaaat aaccaaacaa ggaaaaactt
      841 attttgatac gcggtttgaa gaagaacagg gactatatta tcaaagagga gttcatcgct
      901 accatctgga agaagattgt gaaagcgata cttgggcgat tgctcatcat ctaacatcgc
      961 ttagcccctt gacgattaag cggattgatt ttcaggtctt acagaaaata cttgaaaaaa
     1021 ccacaacaaa agcgtaaaat acattaatgg agggaatacc atgaagacaa acgattcact
     1081 gattgaatta gccttaagaa aaatgcgaaa acccaaaaga gcccgcacca tcgaggatgt
     1141 tgcgaaagat gttttcaaag aaaagagcta tccattcaaa aaggataatg acttattcgt
     1201 ccagtttaat atggacttta tgttgtccgg acattttatt tgttgcgatg aagacaaaaa
     1261 cggcgttaaa ctttgggatt taaaaaatcg ccaaccatcc agtttgttgg ataaagaagg
     1321 aagttattta gacgacttat acaaagacga cgaagaagtt ttgaaaaacg aattaaccga
     1381 tgaagtggcc tttaatcaag gcacggttaa agagttcgaa gcggaaaatt acgatgaaga
     1441 cgatgacgaa gataatgaag aagaacatga cgatatcgaa gaagaacttc taaacgaagg
     1501 cctaacgacg gatgacgaag agaccatcac cgaaaaagtt gttttggacg aagacgacct
     1561 tgatgacgaa gacgatgatg acgacgaact cgattttgcg cacgattaat ttaaaaaaag
     1621 attgttttta attattattt tggtataatt aaaaagagcc aaataagttc tcaaggtttt
     1681 gaggacttat ttttatttga ggaggagatt atgagcacta aatttatttt tgtaaccgga
     1741 ggagtttctt caagtcttgg caaaggtatc aatgcttctt ccatcggcag attgttaaag
     1801 caacgaggcc taaaagtttc catgcaaaag tttgatccct atattaatgt cgataccaaa
     1861 aatatgtccc ccttacaaca tggggaagtt tttgtgactg atgacgg
//
LOCUS       DAOSGN010000172         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_60999_length_1905_cov_2.923784, whole genome shotgun sequence.
ACCESSION   DAOSGN010000172 DAOSGN010000000
VERSION     DAOSGN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60999_length_1905_cov_2.923784"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /gene="obgE"
                     /locus_tag="PK087_02920"
     CDS             <1..1174
                     /gene="obgE"
                     /locus_tag="PK087_02920"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOH18258.1"
                     /translation="GGNGGRGGDIIFVGDEGLTTLLDFRYQKLIVAANGENGRPKNCF
                     GRDAKDVYIRVPVGSIVYDLNNGIVIADITKHKQEVVVAKGGKGGRGNASFGTSRMTA
                     PEICEKGELGEEKVIRIELKILADVGLVGFPSVGKSTIISVVSAARPKIADYPFTTLV
                     PQLGVVKVKDGRSFVMADLPGLIENASQGAGLGFQFLKHIERTRVIVHVLDMDSKEKR
                     NPYDDYLKINKELATYNPQLMLRPQLIVANKMDLPNANHKLEQLRKQIPDMPIIPISA
                     YTNHNLDQMVYEIANLLDTVTIDQFKDDLVDQVIEYRFTPEQAFVIEKDEQGVYNVSG
                     PMVKKFFEATDFTRDANIKMLARRLRNLGVDAELRKLGVKHGDTVRIFGYEFEFLD"
     gene            1254..1871
                     /locus_tag="PK087_02925"
     CDS             1254..1871
                     /locus_tag="PK087_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOH18259.1"
                     /translation="MNNLTKRTTRHEEIRKMVLAAILIAIIVLMTFIPEIGYITIGLV
                     PITIIHIPVIVGGILLGRKYGLILGFVFGLGSMTKSFMEYTVNAPFTNPLLSVVPRSF
                     VGLIASDIYALFTKVIHREKLAVPLALGTVTFIHSLIVLPLLYWVWKSGFFFFTEEFI
                     FALDKKLFPFIWGTFVANSLFEIALAILIGTPLVIVLEILKKNQK"
BASE COUNT          545 a          321 c          433 g          606 t
ORIGIN      
        1 gggaggtaat ggtggtcgcg gtggggacat catctttgtc ggtgatgaag gtttaaccac
       61 tttattggat tttcgttacc aaaaactgat tgttgcagcc aacggcgaaa atggtcgtcc
      121 caaaaactgc tttggtcgag atgccaagga tgtttatatt cgcgttccgg ttggaagcat
      181 tgtttatgat ttaaataatg gcattgtgat tgccgatatt accaaacata agcaagaagt
      241 tgtggttgcg aaagggggga aaggcggacg cgggaacgct tcatttggca cttcgcggat
      301 gacagcaccg gagatttgtg aaaaaggaga gctaggtgag gagaaagtca tccggattga
      361 acttaaaata ttagctgatg ttgggttggt tggttttccg agtgtcggta agtcgacgat
      421 tatttccgtt gtcagtgctg cccgaccgaa gattgccgat tatcctttca ccactttagt
      481 tccccaatta ggggttgtta aagtgaaaga tggtcgtagt tttgtgatgg cggacttgcc
      541 cggattgatt gaaaatgcca gtcaaggggc aggtttaggt tttcaattct tgaaacatat
      601 tgaaagaacg agagtgatag tccatgttct cgatatggat tccaaagaaa agcgtaatcc
      661 ctatgatgat tatttaaaaa taaacaaaga attagcgacc tataatcccc aattaatgtt
      721 gcgaccacag ctaattgtcg ccaacaaaat ggatttgccg aatgccaatc ataaacttga
      781 acaacttagg aagcaaatac ccgatatgcc gattatacct atcagtgctt atacgaatca
      841 taaccttgat caaatggttt atgaaattgc caatttgtta gatacagtta cgattgacca
      901 gtttaaagat gacttggttg atcaagtaat tgagtatcga tttactccag aacaagcctt
      961 tgttattgaa aaagatgaac aaggagtgta taatgttagt ggtccgatgg taaagaagtt
     1021 ttttgaggcg acggatttta cccgtgatgc caacattaaa atgttggcga ggagacttcg
     1081 taatttagga gtcgatgcgg agcttcggaa actgggcgtt aaacatggtg atacggttcg
     1141 tattttcgga tacgaatttg agtttttaga ttagatgtta cttgcgaaag aacatactgt
     1201 aatgagattt taaaaaaact tatgggactt ttaatagcct aaaaaggaga aaaatgaaca
     1261 acttaacaaa acgcaccacg cgtcatgaag aaattcgaaa aatggttttg gcagccattt
     1321 taattgccat tattgtctta atgaccttta ttcctgaaat aggatatatc accattggtt
     1381 tagtgccaat tacgattatc catattcctg tgattgtcgg aggaatttta ctggggcgaa
     1441 aatacggttt aattttgggc tttgttttcg ggcttggttc aatgacgaaa agttttatgg
     1501 agtataccgt aaatgctccc tttacaaatc ctttgctttc agtcgttccc cgctcttttg
     1561 tcgggctaat tgctagcgat atctacgctt tatttaccaa agttatccac cgcgaaaagt
     1621 tagcggttcc cctagccttg gggacggtca cttttattca ttccctcatc gttttaccct
     1681 tgctttattg ggtttggaaa agcggatttt tcttttttac cgaggaattt attttcgcac
     1741 tagataaaaa actattccct tttatttggg gaacatttgt tgctaactca ttgtttgaaa
     1801 ttgccttggc gattttaatt ggtaccccat tagtgattgt cttagagatt ttaaagaaaa
     1861 accaaaaata agtgcggtca ttccgtgctt tttttgttgg ctaat
//
LOCUS       DAOSGN010000173         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_61068_length_1904_cov_2.910222, whole genome shotgun sequence.
ACCESSION   DAOSGN010000173 DAOSGN010000000
VERSION     DAOSGN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61068_length_1904_cov_2.910222"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PK087_02930"
     CDS             complement(<1..1292)
                     /locus_tag="PK087_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18260.1"
                     /translation="MKKSLLKARLRTESRQFVPDLKAQVLSQIPSSQAQRKPRFRLNI
                     KQAWSFSLAVVLIIITGIAYFGLRGINHQTFENSYISVDINPSIELEADGNDLVVSYR
                     SMNIDAQLLLEEDGLNLNGKTIDEAIELIVDLAIEYGYLDVDNPEAAVLVTAINRDTT
                     FEEELNLRLKTKMTALAVKKNLQGEVLLAQADEGMKAEAKAMKVSVGKMILINRARTQ
                     HPDLSVSVAAKLPVKELNEMAKNYNQTKITKFTNDYEQKLANLTSQKEAVLKQMQSKK
                     ATIIETIDEILIMIDNKEPIWTIKTAVDELLATYYPHVKPKNLITYSNYEVFLTNLRD
                     FTEAQVERMGNLVETKYDSQVKAFRFQMQGRLGDELIDFEFVFDNDFKLEEFTDGELS
                     IYNETEERILEIINQISTFISVIDMNPGKQHGRSDRVIS"
     gene            complement(1289..1825)
                     /locus_tag="PK087_02935"
     CDS             complement(1289..1825)
                     /locus_tag="PK087_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOH18261.1"
                     /translation="MFDLMRLEKSMQQLKNGDSSAFEEIYEQTHRMVFYIVYGIVKDY
                     GKAEDLTQDVFMKIYEKIDTYNRSVSAKAWIGMIAKNMAINEYKRRVKQPLMDHDTAD
                     LLRDEKPSRYETPLIDLASKHLSEDEFTIVMLCVGEGYTRKEVGEIFNLSTSGVTWKL
                     KQALDKLRKIAEKEGYGK"
BASE COUNT          546 a          385 c          309 g          664 t
ORIGIN      
        1 gaaataacgc ggtcggagcg tccgtgctgc ttgcccgggt tcatgtcgat aacggaaatg
       61 aaggtagaaa tttggttaat gatttccaaa attcgttcct cggtttcatt ataaatcgat
      121 aattcgccat cagtaaattc ttctagttta aaatcattat caaaaacaaa ctcaaaatca
      181 atcaattcat cacccaaacg accttgcatt tgaaaacgga aggctttaac ttgactgtca
      241 tattttgttt caaccaagtt ccccattcgc tcaacttgag cttcggtaaa atctcttaaa
      301 ttagttaaga agacttcata attgctataa gtgattaaat ttttgggttt tacatgcgga
      361 taataagtag ctaataattc atcaactgcc gttttaatcg tccatatcgg ttctttgtta
      421 tcaatcatga ttaatatttc atcaattgtt tcgataatgg tcgctttttt actttgcatt
      481 tgctttaaga cggcttcttt ttggctagtt aaattagcca gtttttgttc ataatcattg
      541 gtaaatttgg tgattttagt ttgattataa tttttcgcca tttcgttgag ttcttttacg
      601 ggtagtttag cggcaacaga aacgcttaaa tccgggtgtt gagtgcgggc acgattgatt
      661 aaaatcattt tcccaaccga gactttcatc gctttggctt cagctttcat cccttcgtca
      721 gcttgtgcca agaggacttc cccttgtaaa ttcttcttga ccgctaaggc cgtcattttg
      781 gtttttaagc gtaaatttaa ctcttcttca aaagtggtgt cacgattaat tgcggtcacc
      841 aaaaccgcag cttcgggatt atcaacatct aaataaccgt attcgattgc taaatcaaca
      901 attaactcaa tggcttcatc gatggtttta ccatttaaat ttaaaccatc ttcttcgagc
      961 aacaattggg catcaatatt catggaacga taagaaacaa ccaaatcatt accatcagct
     1021 tccaattcaa tggaagggtt aatgtcgact gaaatatacg aattttcaaa ggtttgatga
     1081 ttaatacccc ttaagccaaa ataagcaatt ccggtgataa taattaagac gacagccaaa
     1141 ctaaaactcc aagcttgttt gatatttagt ctaaaccttg gttttcgctg tgcttgcgaa
     1201 ctaggaattt gtgataaaac ttgagctttt aaatcgggaa caaattgccg actttcagtt
     1261 cgtaatcttg cttttagtaa agactttttc attttccata cccctccttt tctgcaattt
     1321 ttcttaattt atctaaggct tgtttcaact tccaagtaac gccgcttgtg gaaagattga
     1381 atatctcccc aacttctttt ctcgtgtaac cttcaccgac acagagcata acaatcgtaa
     1441 attcatcttc ggataaatgt ttagacgcca aatcaatcaa gggcgtttca taacgggacg
     1501 gtttctcatc ccttaataaa tcagcagtat cgtgatccat aagcggttgt tttactcgcc
     1561 ttttatattc attgatggcc atgtttttcg caatcattcc aatccatgct ttggcggaaa
     1621 cactgcggtt gtaggtatca attttttcat atattttcat aaaaacgtct tgcgttaaat
     1681 cttcggcttt cccatagtct tttacaatcc cataaacgat gtaaaaaacc attcgatgcg
     1741 tttgctcgta gatttcttca aaagccgaag aatcgccgtt tttaagttgt tgcatacttt
     1801 tttctaagcg cattaaatca aacatcgttt ccccctaaaa ctggtttagt ttcttgctcg
     1861 ctaatataac aaacaacaat ccttttttgc cagtattttc aaat
//
LOCUS       DAOSGN010000174         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_61343_length_1898_cov_3.486164, whole genome shotgun sequence.
ACCESSION   DAOSGN010000174 DAOSGN010000000
VERSION     DAOSGN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61343_length_1898_cov_3.486164"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    327..527
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            560..1552
                     /gene="trpS"
                     /locus_tag="PK087_02940"
     CDS             560..1552
                     /gene="trpS"
                     /locus_tag="PK087_02940"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOH18262.1"
                     /translation="MKIILSGVKPTGIPTLGNYLGALRNFGKFQEKMKDYEFFIFVAD
                     LHAITTPQDPLELKQNIRNVAALYLACGLKPENLTLFIQSEVHEHAEMAYIMQALVYM
                     GELERMTQYKDKVLKQTQGVSSALFTYPALMAADILLYDAKFVPVGDDQRQHLELARD
                     LAIRFNNRFGKTFTVPEGLIQEKGARIKDLQDPTKKMDKSTDNDRGCIFLLEPLSQIR
                     KKIKAAVTDSDACVRFDPEQKPGIANLMTIYSVITEKSFGEIEAMYDGKGYGDFKSDL
                     AEIVVKEIAMIQERYHYFVSSQELTTLLDEGRLRAHQLAQKKLKTVYQKMGLGR"
     gene            complement(1564..1845)
                     /locus_tag="PK087_02945"
     CDS             complement(1564..1845)
                     /locus_tag="PK087_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18263.1"
                     /translation="MQTLATVGSVGFLTFIFLILAIAKKALSHSLFNICVFFSLLAMI
                     IHGLMDNTFHNPLIMLIVAFLAALLEEAPPLSIKTLFRKNPKEVRKNPD"
BASE COUNT          613 a          347 c          392 g          546 t
ORIGIN      
        1 tctgggccag caagggaaag agtatactct ttgtcttcct tacagccaac aaaaactaac
       61 gcaagagcca aaaagaaaaa taaacttaag aattttttca ttttttcctc ccattttttt
      121 cactcatgca gatagcattc tgtgattaat catattatac acatcaagga tgaaagtgtc
      181 aacaaatatt tttttagtaa gcgctttatt ttcatctttt ttcatttttt cagttgtttt
      241 acaacaaaga aatcgcttac attggctttt tcccttcttt ttaaaaacgg ctcttgaaaa
      301 tgaactttta aggtgtataa taataactag aaatgatgaa gagaaatagt acgggtgttg
      361 ttttggaaaa agcgaatgct ggatagtgaa agcagcatca aaaggcagcc ggaatggatc
      421 ttggagtgca acgaggaaat gcgttgacga tggagttcgc gttaagaact ttaagggtat
      481 tggcaacaat aaattaaggt ggtaccgcgg aatctttcgt ccttaacata gtttaaggac
      541 tttttttatt ggaggaagta tgaagattat tttatcgggt gttaagccca ccggcattcc
      601 cactttaggg aattatttag gagctttaag gaattttgga aaattccaag aaaagatgaa
      661 ggattatgag ttttttattt ttgtggcgga tttacatgca ataacaactc cgcaggatcc
      721 tttagaactc aaacagaaca ttcgtaatgt cgccgctttg tatttagctt gcggtttgaa
      781 accggaaaat ctaactttgt ttattcaatc ggaagtgcat gaacatgccg aaatggccta
      841 tattatgcaa gcattagtct atatgggcga actggagcgc atgacccaat acaaagacaa
      901 agttctcaaa caaacacaag gcgtcagttc agcattattt acttatcccg ctttaatggc
      961 ggctgatatt ttactatacg atgctaaatt tgtgccggtt ggggatgatc aacgccaaca
     1021 tttggaatta gcccgcgatt tagccatccg ctttaacaac cgtttcggaa aaacctttac
     1081 cgtcccggaa ggtttgattc aggaaaaagg ggctcgcatt aaggatttac aagatcccac
     1141 taaaaaaatg gataaatcca ctgacaacga ccgcggttgt attttcctgt tagaaccgct
     1201 ttcccaaatt cgtaaaaaaa ttaaagcggc ggtaactgac agcgacgcct gtgtacgctt
     1261 tgatccggaa caaaaaccgg gtattgccaa tttaatgacg atttattcgg tgattactga
     1321 aaagtcgttt ggggaaatcg aggcgatgta tgacggtaaa ggctatggcg atttcaagag
     1381 cgatttagcg gaaatagtcg ttaaagaaat tgccatgatt caagaacgct atcactactt
     1441 tgtttcatca caagaactaa ctaccctcct tgatgaaggg cgcttacgcg cccatcaact
     1501 ggcccaaaaa aagctgaaaa ccgtttacca aaaaatgggg ctggggcgtt aaaaaatccg
     1561 gatttaatcc ggatttttcc ttacttcctt ggggtttttg cgaaaaagag ttttgatgct
     1621 tagcggtggt gcttcttcca aaagcgccgc caaaaaggca actatcagca tgatgagggg
     1681 attatggaaa gtattatcca tcaatccatg aataatcatt gccaaaaggg aaaagaaaac
     1741 acaaatatta aataaactgt gcgaaagagc tttcttcgca atcgccaaga ttaaaaagat
     1801 aaaggttaaa aaaccaacac ttcccaccgt cgctaatgtc tgcatcacaa agctgtgata
     1861 atgtttaagt aagggtgcat caaactagag tgttaaat
//
LOCUS       DAOSGN010000175         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_61544_length_1894_cov_2.292007, whole genome shotgun sequence.
ACCESSION   DAOSGN010000175 DAOSGN010000000
VERSION     DAOSGN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61544_length_1894_cov_2.292007"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PK087_02950"
     CDS             complement(<1..887)
                     /locus_tag="PK087_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019720824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOH18264.1"
                     /translation="MKKLKSYEIRQIWLDFFKSKNHEIIESAPLVPINDPTLLWINAG
                     VAPLKKYFDGREVTKHKRMANAQKSIRTNDIENVGKTARHHTFFEMLGNFSIGDYFRE
                     EALTWAIEILTSPQWYGFDLSKLYFTVYPEDTESVKKWESLGVPRDHIIPLEGNFWEI
                     GEGPCGPDTEIFYDRGEKYDPHGLGISMLKNDIENDRYIEIWNIVFSQYNSIPGLDRH
                     DYPELPSKNIDTGMGLERMACVIQEVDTNYDTDLFMPIIKATEMIVGTKYQNQMAFKV
                     IADHVRCVVFAIADGALPS"
     gene            complement(893..1699)
                     /locus_tag="PK087_02955"
     CDS             complement(893..1699)
                     /locus_tag="PK087_02955"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOH18265.1"
                     /translation="MIAIIITGVRHEPYYTLQRMAFGNDRLVHLNEQDHIVIVSQPTT
                     GTELLAQKTLSVLHRLNSQITVIAKDQLPSSHADNEDLKMMYELLQPKYIIPVCGEYR
                     HQFMQKHVAMEAGYPEDKILVLDNGEMVSFQDGVYGNKKTQISVGDVLIDGSIVGDIN
                     EIVLKDRELLGSEGIILVVTTIDARTKQIIAGPKVVLKGFFVGETANDVISSLSELSE
                     TLINKYLNRRQIDWNELKGLLREAINKDIYELTKKNPIIMPVLIDVEKKE"
     gene            complement(1665..>1894)
                     /locus_tag="PK087_02960"
     CDS             complement(1665..>1894)
                     /locus_tag="PK087_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18266.1"
                     /translation="MFSNELQRIQKVINVCVAQNRRIAIIGKKAQKTINVAINYDYLK
                     IPPQNSINLKYITEENQNNEDDSHHYHRCSP"
BASE COUNT          580 a          387 c          303 g          624 t
ORIGIN      
        1 ttacttggca aagcaccatc ggcaatggca aaaaccacac aacgaacatg gtcagcaatg
       61 accttaaatg ccatttgatt ttgatatttc gtcccaacaa tcatttccgt tgctttaata
      121 atcggcataa acaaatcggt atcataattg gtgtccactt cctgaataac gcaagccatt
      181 cgttccaagc ccatcccagt atcaatgttc ttgcttggaa gttccggata atcatgacga
      241 tctaaaccgg gaatagagtt atattgactg aagacaatat tccatatttc aatataacga
      301 tcattttcaa tgtcgttttt caacattgaa attcccaaac cgtgaggatc atatttttcg
      361 ccacggtcat aaaagatttc cgtgtccggt ccacatggcc cttcaccgat ttcccaaaaa
      421 ttaccttcta aaggaataat atgatcacgg gggactccta aactttccca tttttttacc
      481 gattccgtat cttcagggta aaccgtgaaa tatagtttac ttaaatcaaa gccataccac
      541 tggggagaag tgaggatttc aatggcccaa gtgagagctt cttcacgaaa ataatcacca
      601 atggaaaaat tacccaacat ttcaaagaaa gtatggtgac gagcggtctt tccgacattt
      661 tcaatatcat tagtgcggat agatttttgg gcattggcca tccgtttatg tttagtaact
      721 tcccgaccat caaaatattt ttttaggggt gcaaccccag cattaatcca caataaagtc
      781 ggatcgttaa tagggactaa gggagcactc tcaatgattt catgattttt acttttaaaa
      841 aaatctaacc atatttgtct tatttcataa ctttttaatt ttttcatatt tactattctt
      901 tcttttcgac atcaattaaa acaggcatga taatgggatt tttcttggta agctcataaa
      961 tatctttatt aatcgcttcc cgtaataagc ctttcaattc attccaatca atttgacggc
     1021 gatttaaata tttgttgatt aatgtttccg ataactcaga aagcgaacta atgacatcat
     1081 tggctgtttc accaacaaag aagcctttta acaccacttt gggaccagca atgatttgtt
     1141 ttgtgcgggc atcaatcgtc gtaaccacta aaataatccc ttcactgccg agcaattcac
     1201 gatctttgag gacaatttca ttaatatcgc caacaatcga accatcaata agaacatctc
     1261 caaccgagat ttgcgttttt ttgttaccat aaacaccatc ttggaagctt accatttcac
     1321 cattgtctaa cactaatatc ttatcttccg gatatcccgc ttccatcgca acatgttttt
     1381 gcataaactg atggcgatat tccccacaca ccggaataat gtatttcggt tgcaataatt
     1441 catacatcat ttttaaatct tcattatctg cgtgcgagct tggtaattga tctttagcaa
     1501 taactgttat ttgagaattt aaacgatgta aaacgctcaa agttttttgg gctaataatt
     1561 ccgttcccgt agttggttgg gaaacaatca caatatgatc ttgctcattc aagtgaacta
     1621 aacgatcatt tccaaaagcc atccgttgta aagtgtaata aggttcatgg cgaacaccgg
     1681 tgataatgat ggctatcatc ttcattattt tgattttctt cggtgatgta ttttaaatta
     1741 atgctgtttt ggggaggaat ttttaaataa tcataattaa tcgccacatt gatagttttc
     1801 tgagcttttt taccaataat agcaattcga cgattttgtg ctacacaaac attaatgact
     1861 ttttgaattc gctgcaactc gttagaaaac atgg
//
LOCUS       DAOSGN010000176         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_61571_length_1893_cov_3.591404, whole genome shotgun sequence.
ACCESSION   DAOSGN010000176 DAOSGN010000000
VERSION     DAOSGN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61571_length_1893_cov_3.591404"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1209)
                     /gene="ugpC"
                     /locus_tag="PK087_02965"
     CDS             complement(88..1209)
                     /gene="ugpC"
                     /locus_tag="PK087_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOH18267.1"
                     /translation="MATVTMKNLNKVYDGNVHAVADFNLHIKDREFIVFVGPSGCGKT
                     TTLRMVAGLEDITSGELRIDDEVVNDVAPKDRNIAMVFQSYALYPHMTVYDNMAFGLK
                     LRKFSRDEIDRRVQNAAEILGLKPYLDRRPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRAEIIKLTERLGITTIYVTHDQIEAMTMATRIVVMKDGYIQQIGS
                     PKEIYDFPNNIFVGGFIGTPPMNFLEGKVYEDGFFEMGDHKLGVSRIKVPKDKFEMLK
                     EQKLLEKTIILGIRPEDIHDTPEQIAHHKDSVVKLKVDVAELLGAETLINGVLHGQGL
                     VAKVKARVDIHMGDDIEMALAMDKSHFFDPETEQRVKKY"
     gene            complement(1288..>1893)
                     /locus_tag="PK087_02970"
     CDS             complement(1288..>1893)
                     /locus_tag="PK087_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH18268.1"
                     /translation="KQAYPFSNRPYYFLVIKMVDPSQEQEVVNFLEELWPKTYFLKLK
                     QELIVFYFEEYDFKNVMVSMSDDFSWSFSCFQSGKMNPDYPEYFHILLDTLHLQFQNR
                     SYFYLDIVDLIMHLVKTNRSLLRPLVPIILNRVYKDSQYERIIKAMFEHDLNVTKAAQ
                     SVYMHRNTMINKLEFIKTETGLNIQHFKPAFCMYWLLDAQK"
BASE COUNT          580 a          407 c          321 g          585 t
ORIGIN      
        1 ccggtattca acaacttctt ttgttacctt taaatataaa gtcctacctt ccataaaaaa
       61 gccacctttc acaggtggct agatcgtcta gtatttcttt actctttgtt ctgtttccgg
      121 atcaaagaaa tggcttttgt ccatggcaag ggccatttcg atatcgtcac ccatatgaat
      181 atcaacgcga gctttaactt tcgcaaccaa tccttggccg tgtaaaaccc cattgattaa
      241 ggtttcggcg ccaagtaatt ccgcaacatc aacttttaat ttcacaaccg aatccttgtg
      301 atgagcaatt tgttccggtg tatcgtgaat atcttccgga cgaataccga ggataatcgt
      361 tttttccaac aatttttgtt ctttcaacat ttcgaatttg tctttcggaa ctttaattct
      421 cgatacccca agtttatgat cgcccatttc aaagaaaccg tcttcataaa ctttgccttc
      481 caagaaattc attggcggcg tgccaataaa cccaccgacg aaaatattgt tcgggaaatc
      541 ataaatttct ttcggagaac cgatttgttg gatataaccg tctttcataa caacaattct
      601 ggttgccatg gtcatggctt caatttggtc gtgagtaaca tagatggtgg taatccccaa
      661 acgttccgtc aatttaataa tttcggcccg catttgcacc cgcaatttcg catcaagatt
      721 ggataagggt tcgtccatca agaaaacttt agcgtcacga acaatcgctc gacctaaagc
      781 aaccctttgt ctttgcccac cggacaaagc tttcggacga cgatccaaat agggtttcaa
      841 acctaaaatt tcagcggcat tttgcacccg gcgatcgatt tcatcacgag agaatttgcg
      901 caattttaaa ccgaaagcca tgttgtcata aaccgtcata tgcggataca aagcatagct
      961 ttggaaaacc atggcgatgt tccgatcttt gggagctaca tcgttaacga cttcatcgtc
     1021 aatccgtaat tcaccggaag taatatcttc caaaccggcg accatccgta atgtcgttgt
     1081 ttttccacat cccgaaggac caacaaacac aataaattct ctgtctttaa tgtgtaagtt
     1141 aaagtcagct acggcatgga cattgccatc ataaactttg tttaagtttt tcattgttac
     1201 agttgccata aataatctcc tttatttaaa tgctagcatt attataaaga aaatctttct
     1261 taaaaacaat gtgcaacttg cacaaactta tttttgagca tccaaaagcc aatacataca
     1321 aaaggcgggt ttaaaatgtt ggatgtttaa gccggtttcc gttttaataa actccagttt
     1381 attaatcatc gtattgcggt gcatataaac actctgggcg gctttcgtaa catttaagtc
     1441 atgttcaaac atcgctttaa ttatgcgctc atactgggaa tccttataca cccggttaag
     1501 aataatcggc accagtggcc ttaataaaga acgatttgtt ttcaccaaat gcataatcaa
     1561 atcgacaata tctaaataaa aataacttcg gttttggaac tgaagatgta aagtatctaa
     1621 gagaatgtga aagtattccg gataatccgg gttcatcttt ccgctttgaa aacaactgaa
     1681 agaccaggaa aagtcatcac tcatcgacac catcacattt ttaaaatcat attcttcaaa
     1741 ataaaaaacg attaattctt gtttcagctt aagaaaataa gttttaggcc ataattcttc
     1801 taaaaaatta accacctctt gttcttggct ggggtcaacc attttaatga ctaaaaaata
     1861 ataaggccgg tttgaaaagg ggtaggcttg ctt
//
LOCUS       DAOSGN010000177         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_61708_length_1891_cov_2.907407, whole genome shotgun sequence.
ACCESSION   DAOSGN010000177 DAOSGN010000000
VERSION     DAOSGN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61708_length_1891_cov_2.907407"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PK087_02975"
     CDS             <1..843
                     /locus_tag="PK087_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18269.1"
                     /translation="ETHLASFDEEVNRAYAEYQVYDEYANSYYAIKLVYGIVNDKMSY
                     GVSFFSEQAQAFTLKIAYQNQVYLLPTDNRGDIKLIALNLKEASTFSLLVYDKDNHLQ
                     ALNRFQNIEVVPKNDFLNLPNHNVGLNEGVKFVSLNSINWSPLTIFYLGLLVVFMICA
                     VVIIIFYWRKKGFFEKENRESFFEVEVIPSAEAVNGEEETFVQTPLIEPIKEEQFIWE
                     DDDEQSKIDIPSHLQSLGLSTQYQDLSEMERFKIMEVLIKLRDEKLITNDDYLKEVAK
                     LWRK"
     gene            831..1388
                     /locus_tag="PK087_02980"
     CDS             831..1388
                     /locus_tag="PK087_02980"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOH18270.1"
                     /translation="MEKISLKDLLKLSKDDLQGKIICFPTDTVYGVGALYNDEVAIKK
                     IYAMKKRDVGKPLVNLCSSIDQITDLGIVIDAQAQSLMNKHWPGALTIVLCHQEKTIA
                     FRMPDSPIARALVDRFSLLATTSVNESKQAELNSLREIENQFFNDIDYLVTDAMSFSQ
                     IPSTVVKVCAGKISVLRQGQIDVFK"
     gene            1471..>1891
                     /gene="rpiB"
                     /locus_tag="PK087_02985"
     CDS             1471..>1891
                     /gene="rpiB"
                     /locus_tag="PK087_02985"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOH18271.1"
                     /translation="MIISIACDHGGFPLKTELVKILRAKNYEVLDRGSYDLNRVDYPE
                     YAKAVCEDVVSKTASFGVLICTTGIGMSIYANKWKGIRAALVTNLESAHLTRQHNDSN
                     IITMGARFTSIEDASLYVEAFVNESFEGDICTVTIAMP"
BASE COUNT          590 a          302 c          394 g          605 t
ORIGIN      
        1 gaaacccatt tagcatcatt tgatgaagag gtcaatcgtg cttatgcgga ataccaagtt
       61 tatgacgaat atgccaattc ttattatgcc attaaattgg tgtatggaat tgtgaatgat
      121 aaaatgagct acggagttag cttttttagc gagcaagcgc aagcttttac tttaaaaata
      181 gcttaccaaa atcaagtcta tcttttaccg actgataatc gtggtgacat taagcttatt
      241 gccctcaatt taaaagaagc ttcgactttt tctttgcttg tttatgataa agataatcat
      301 ctccaggctc ttaaccgctt tcaaaatatt gaagtggtgc cgaagaacga ctttttaaat
      361 ctccctaatc acaatgttgg tttaaatgaa ggcgttaaat ttgtcagtct aaacagtatt
      421 aattggtcac ctcttaccat cttttatttg ggtttgctgg tggtttttat gatttgcgca
      481 gtcgttatca ttatctttta ttggcgcaaa aaaggtttct ttgagaaaga aaatagagaa
      541 agtttttttg aagttgaagt tatccccagc gctgaagcag ttaacgggga agaagaaact
      601 tttgtacaaa cacctttaat tgaaccaatt aaggaagaac aatttatttg ggaagatgat
      661 gatgagcaaa gcaaaattga tattccttcc catcttcaaa gtttaggctt aagcactcaa
      721 tatcaagatt tatcggaaat ggaacgcttt aagattatgg aagttttaat caaacttcgt
      781 gatgaaaaat taattacgaa tgatgattat ttaaaggaag ttgcgaaatt atggagaaaa
      841 taagtttaaa ggatttattg aaattatcaa aagatgattt acaaggaaaa attatttgtt
      901 ttcccaccga tactgtctat ggagtagggg ccttatataa tgatgaagtg gctatcaaga
      961 aaatctatgc gatgaaaaaa cgcgatgtgg gaaaaccgct cgtcaatttg tgttcctcca
     1021 tcgatcaaat tactgactta gggattgtca ttgatgcaca agcccaaagc ttaatgaata
     1081 agcattggcc gggtgcttta acgattgttt tgtgtcatca agaaaaaacg attgctttcc
     1141 gcatgcccga cagtcccatt gcgcgcgcct tagttgaccg tttttcgttg ttggcaacaa
     1201 catcggttaa cgaaagtaag caagcggaac ttaactcgct acgagaaatt gaaaaccagt
     1261 tttttaatga tatcgattat ttagttactg atgcgatgag tttttcccaa attccctcaa
     1321 cggttgtgaa agtatgtgct gggaaaataa gcgttcttcg tcaaggccaa attgatgttt
     1381 ttaagtaaaa tcacgaaagt gatttttttt tgcttttggg gtagataaat aaacaaaaat
     1441 atagtataat tcctaactag aggtgaaaag atgattatca gcattgcttg tgaccatggt
     1501 gggttccctt taaaaacgga gcttgtaaag attttgcggg ccaagaatta tgaagttctt
     1561 gaccgtggca gttatgatct taatcgtgtc gattatccgg aatatgcgaa agcggtttgc
     1621 gaagatgtcg tttctaaaac ggcttcattc ggggtgttaa tctgtacaac gggcattgga
     1681 atgtccattt atgcgaataa atggaagggg attcgggcag ctttggtgac taatctcgaa
     1741 agcgctcatt taactcgtca gcataatgac agtaatatta ttaccatggg cgcccgtttc
     1801 acaagcattg aagacgctag cctttatgtt gaagcctttg taaatgaaag ttttgaaggc
     1861 gatatttgta cagttacgat tgcaatgcct c
//
LOCUS       DAOSGN010000178         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_61719_length_1891_cov_2.338235, whole genome shotgun sequence.
ACCESSION   DAOSGN010000178 DAOSGN010000000
VERSION     DAOSGN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61719_length_1891_cov_2.338235"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PK087_02990"
     CDS             <1..462
                     /locus_tag="PK087_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOH18272.1"
                     /translation="NSVVAQLLFLAAEDAEKDIYIYINSPGGSITAGMAIYDTMNLVA
                     PDVQTICVGIAASMAAFLLASGTKGKRFALPNSEILIHQPMGGASGQATEIQIAAKHI
                     LRVKERVNQILAEKTNKTIEQINQDTERDNYMFSEEALAYGIIDHIMTKKS"
     gene            complement(524..1270)
                     /locus_tag="PK087_02995"
     CDS             complement(524..1270)
                     /locus_tag="PK087_02995"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003424.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOH18273.1"
                     /translation="MKYAQFGNYEALNIKMIRDDEHPELVFSFGGDGTMLAAIHRYLD
                     QLEQVKFVGINTGKLGFFTDFDKDELPLICEMLEKGDYEINKFHILEYTLINQYQSIT
                     GYAVNELAIISPVHTQNIDVFINDEHFETFRGTGLLVSPPTGSTAYNKSLGGSVIDPH
                     IRAIQLTEIAPINNRLFKSLSSPLVLSEKSKIALIVSENQNLVISADGVLLDFKDVQK
                     VMTKLSKRTVSFVVKPNTDFFHRVKRSFIK"
     gene            complement(1275..>1891)
                     /locus_tag="PK087_03000"
     CDS             complement(1275..>1891)
                     /locus_tag="PK087_03000"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HOH18274.1"
                     /translation="FIIKLEAMRKEFLFKKVNNPIEIVSGRVKSVSSILEKAERLNID
                     FNHIPEGIYDIAGIRVTCKYIEDVYTVADLLKSRKDIDIFLIKDYIKEPKASGYRSLH
                     LIAKYNVETVDGRMPINIEFQIRTHAMHLWASIEHTLKYKYYRHIPEEIQERLIQASI
                     ICASLDDEMTRIKSTMGSVVVDKVRETEPDLDEMEVFLNSIKKW"
BASE COUNT          604 a          403 c          329 g          555 t
ORIGIN      
        1 aattcggttg ttgctcaact gttattttta gcagcggaag atgccgaaaa agatatttat
       61 atttatatta actctcccgg tggctcaatc accgccggaa tggcaatcta tgacaccatg
      121 aacttggtgg cccctgatgt ccaaacgatt tgtgttggca tcgccgcttc catggccgcc
      181 tttctattgg ccagtggtac caaaggaaaa cgttttgcct tacccaacag tgaaattctc
      241 attcaccaac caatgggagg agcatccgga caagcgacgg aaattcaaat tgcggccaaa
      301 catatcttgc gagtcaaaga acgcgtcaat caaattctag cggagaaaac caataaaacc
      361 atcgaacaaa tcaatcagga tacggaacgc gataactata tgttttccga agaagcctta
      421 gcttatggga ttattgacca cattatgaca aaaaagagct aaagattttt aaatctttaa
      481 ctctttttaa ttatgataaa acccattatt tgataatgga acattattta atgaaggaac
      541 gcttaacccg atgaaagaaa tccgtattcg gcttcacaac aaacgaaaca gtacgcttgg
      601 ataatttcgt cattactttt tgtacatcct taaaatccaa caatacacca tcagcactaa
      661 taactagatt ttggttttcg gaaacgatta aggcaatttt agatttttcg gacaacacta
      721 agggtgaaga caaactttta aaaagccgat tgttgatggg agcaatttcc gttagttgga
      781 tagcacgaat atgcggatca ataacactcc cgcccaaaga tttattatag gccgtacttc
      841 ctgttggcgg agatactaat aaccccgtcc cccgaaaagt ttcaaaatgt tcatcattga
      901 taaaaacatc aatgttttgg gtatgaaccg gactgataat cgccagttca ttaaccgcat
      961 aaccggtgat gctttgatat tggttaatta atgtgtattc caaaatatga aacttattta
     1021 tttcataatc gcctttttcc aacatttcac agattaaggg taattcatcc ttatcaaaat
     1081 ccgtaaaaaa ccccaattta cccgtattaa tgcccacaaa cttcacttgt tcaagttgat
     1141 caagatagcg atgaatggcc gcaagcatcg tgccatcgcc accaaacgaa aaaacaagct
     1201 cgggatgttc atcatcacga atcattttaa tgtttagggc ttcataattt ccaaactgag
     1261 catatttcat atcatcacca ttttttaata ctatttaaga aaacttccat ctcatccaag
     1321 tccggttccg tttcccgaac tttgtcaacg acaaccgagc ccatagttga cttgatgcgc
     1381 gtcatttcat catctaaact ggcacaaata atggaggctt gaatcagtcg ctcttggatt
     1441 tcttcgggaa tgtgacggta atatttatat ttcaaggtgt gttcaatgga ggcccacaaa
     1501 tgcatggcat gagtccgaat ttgaaactcg atattaatag gcatccggcc atcaacggtt
     1561 tccacattat acttggcgat taagtgtaaa gaacgataac cactggcttt cggttctttg
     1621 atataatctt taatcaaaaa aatgtcaatg tctttacggg acttcaaaag gtccgccacc
     1681 gtataaacat cttcaatata tttacaagta acccgaatcc cggcaatatc gtaaatgcct
     1741 tcgggaatat ggttgaaatc aatattcaac cgttccgctt tttctaaaat gctgctcact
     1801 gacttgactc gacccgagac aatctcaatg ggattgttaa cttttttaaa caaaaattcc
     1861 ttacgcattg cttcaagctt gataataaat c
//
LOCUS       DAOSGN010000179         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_61730_length_1891_cov_1.675926, whole genome shotgun sequence.
ACCESSION   DAOSGN010000179 DAOSGN010000000
VERSION     DAOSGN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61730_length_1891_cov_1.675926"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PK087_03005"
     CDS             complement(<1..223)
                     /locus_tag="PK087_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18275.1"
                     /translation="MKRWALFALVMCLLLLSGCYNYSAGSQYGKIVGISFFDEEGEEI
                     PGQFYDYFSFQEQFEDDTFLSLSNRLIPLN"
     gene            complement(234..581)
                     /locus_tag="PK087_03010"
     CDS             complement(234..581)
                     /locus_tag="PK087_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18276.1"
                     /translation="MSEITPMIFETLLKALALTLVCELVVLMLFRCFKQLYLVAILIN
                     IFTNIGMNLLILWVNPIHYHVFVIFMEIIVILIEFLIYYLFIKKGKQALLISLAANFT
                     SYLVGLALMGLIY"
     gene            complement(714..>1891)
                     /locus_tag="PK087_03015"
     CDS             complement(714..>1891)
                     /locus_tag="PK087_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH18277.1"
                     /translation="IFIGINDKNKELVDVVDYAKLKLKIENKINDSITPRPRYEINAL
                     SYGEKTILEILVYPGPNKPYMYKGVAYQRSDTSTIPVDQTSLVELSLKGKNISYDQLE
                     TNEEELQFTVLDKKLNEVRTVSNFNKDILITLGLFTNDKYNIAASLLADKNNFKTLGI
                     DIVRFGQTISEFIDRKTIVGESILLQYDGAIEMFAKHYPDIEIVTGMKRIKKQPVPYE
                     AYREAIANAIAHRNYLIPSNIQIKMYNNRIEVISPGGLPNGLTKENYLKDNLSVPRNT
                     IISQILHILGIIEKFGTGIRRINEAYFGYDKKPSFVIKDNFIKVILPNILFNDEDKNE
                     EARILNLIDIKTEITRQDVENLLSVNKAKAVELLNKLKEDCLIESIGTGKNVVYQKK"
BASE COUNT          622 a          334 c          276 g          659 t
ORIGIN      
        1 aatttaaggg tatgaggcga ttacttaatg aaaggaaggt atcatcttca aattgttctt
       61 ggaaagaaaa atagtcgtaa aattgaccgg ggatttcctc gccttcttca tcaaaaaaac
      121 tgatgccgac aattttacca tattgcgaac cggcactgta attataacag ccggataaca
      181 acaataagca catcacaagt gcaaaaagag cccatctttt catgtttccc tcctcaataa
      241 attaagccca tcaaggctaa tccaacaaga taacttgtga aattagccgc tagagagatt
      301 aatagggctt gttttccttt ttttataaat aaataataaa tcaaaaactc aattaagata
      361 acaattatct ccatgaagat gacaaataca tgataatgaa tcggatttac ccataaaata
      421 agcaagttca ttccgatgtt agtaaagatg tttataagaa tagcaacaag atagagttgc
      481 ttgaaacagc gaaaaagcat gaggaccact agctcacaaa ctaaagttag agccaatgcc
      541 tttaataatg tttcaaatat cattggggtg atttcactca tactcttttt ccgcaactaa
      601 caattgctta taactaatag cattatacca aagaaaaatg ggcaactcaa ctagatattc
      661 ctttaaaaat tgatatatgt cgacttgccc ttaagcattt ccgctcaagg aatctatttt
      721 ttttgataga caacattctt accggtccca attgattcga ttaaacaatc ttcttttagt
      781 ttgtttaata attcgactgc ttttgcctta ttaacactaa gcaagttttc tacatcttgt
      841 ctcgttattt ctgttttaat atcaattaag tttagaatcc ttgcttcttc attcttatct
      901 tcgtcattaa ataaaatatt cggtaggata actttaataa agttgtcttt gataacaaaa
      961 ctaggttttt tatcatatcc aaaataggct tcattaatcc ttcttatgcc cgttccaaac
     1021 ttctcaataa ttcccaagat gtgcaatatt tgggatataa tagtgtttct tggaactgat
     1081 aagttgtcct ttaaatagtt ctctttagta aggccattgg gaaggccacc cggagatatg
     1141 acctcgatac gattattata catttttatt tggatattag atggtattaa ataatttcga
     1201 tgggcaatcg cgttggcaat ggcctcccga taggcttcat aaggcaccgg ttgttttttt
     1261 attcttttca tccccgtaac tatttcaata tctggataat gttttgcaaa catttcaata
     1321 gcgccatcat attgtaataa aatcgattca ccaacaatcg tttttcggtc aataaattcg
     1381 gagattgttt gaccaaaacg cacaatatca atccctaatg tcttaaaatt atttttgtca
     1441 gcaagcaatg aagcggcaat attatatttg tcgttggtga ataatcccaa agttattaaa
     1501 atatctttgt tgaaattaga aactgttcta acctcattaa gttttttatc taaaactgtg
     1561 aattgtaatt cttcttcatt tgtttctaat tgatcgtatg aaatattttt gccttttaac
     1621 gacaattcta ccaaggatgt ttgatcaacg ggaatagttg atgtatcact tctttgataa
     1681 gcaactcctt tatacatata tggcttatta ggacccggat aaacaagaat ttccaatatc
     1741 gttttttccc cataactcaa agcatttatt tcataacgag gtcttggcgt aatggaatca
     1801 ttaattttat tttcaatttt caattttagc ttggcatagt cgacaacatc aacaagctct
     1861 ttatttttat cattaatacc aataaaaatt t
//
LOCUS       DAOSGN010000180         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_62128_length_1883_cov_3.925055, whole genome shotgun sequence.
ACCESSION   DAOSGN010000180 DAOSGN010000000
VERSION     DAOSGN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62128_length_1883_cov_3.925055"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PK087_03020"
     CDS             <1..338
                     /locus_tag="PK087_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOH18278.1"
                     /translation="NVHLHNDSRNLFPHVYLDTDQEFVWPEQSTTTSPFVFCRGLKDH
                     IAILVDGSVVPCCLDGNGQVVLGNVFSDSLKTILAGSAWKKLREAFKKRQNLPPLCQH
                     CSYKDRFAL"
     gene            414..749
                     /locus_tag="PK087_03025"
     CDS             414..749
                     /locus_tag="PK087_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOH18279.1"
                     /translation="MIKKGDIVIGKITNILGYGAFVSVGEYDGLIHISEFSDNFVRKI
                     DDYVQVGQQVKLKVLDVDENTKKLRLSYKTLNKTRGVKGEIPKFIIGFNSLEKAMPAF
                     IAAQIKKKE"
     gene            751..>1883
                     /locus_tag="PK087_03030"
     CDS             751..>1883
                     /locus_tag="PK087_03030"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOH18280.1"
                     /translation="MLNYQQVLPFIKKTDLEALEPQALKAKEDLLHKTCLGNDFVGWV
                     DYPNRLSEADLKAIVAASQTIQNQSQVLLVIGIGGSYLGAKSALSMLRAYFKKTGGLE
                     ILFVGNTLSSTYTKAVLQYLQHKDFSINVISKSGTTTEPAIAFRLFRGLLKEKYGSRF
                     NERIYCTTTIGKGVLYSEAINNNYQIFAIPEDIGGRYSVLTPVGLLPMACSNIDIYQV
                     INGASQAIFNFSQKPFWENQVLLYASLRKALYDQGKRIEMLVYYEPKLHYFGEWYKQL
                     FGESEGKNHLGLFPVTANYSTDLHSLGQYVQDGHRHLFETVLNINKPEEDLTIPLDQL
                     DFDQLNYLTKYSLHQINHQALVGTTMAHVDGGVPNILINVPELS"
BASE COUNT          560 a          332 c          394 g          597 t
ORIGIN      
        1 ttaatgttca tttgcacaat gattcaagga atttgtttcc ccatgtttat cttgatactg
       61 atcaagaatt tgtgtggccg gaacaatcaa ccacgacgag tccgtttgtt ttttgtcggg
      121 gtttaaaaga tcacatcgct attttagtgg acggttccgt tgttccttgt tgcttggacg
      181 gtaatgggca agtggtgtta ggaaatgtgt tttccgattc cctaaaaacg attttggcgg
      241 gatcggcttg gaaaaaatta agggaagctt ttaaaaaaag acaaaacttg ccaccgcttt
      301 gccaacattg ttcatataaa gaccgttttg ccctctaggg cattttttat ttctccttaa
      361 cttttataat aacatatgtt ataatttaca tatagtaggt caagaggtgg acaatgatta
      421 aaaaaggtga catcgtcatc gggaagatta ccaatattct cggttacggt gcttttgtga
      481 gcgtggggga atatgacggc ctaattcata tttccgaatt ttccgacaat tttgttcgta
      541 aaatcgatga ttatgtgcaa gtcggacaac aagtgaaact gaaagtattg gatgttgatg
      601 aaaatactaa gaaattacgt ttaagctata agactttgaa taaaactagg ggtgtcaaag
      661 gtgagattcc gaaatttatt atcggattta attctttaga aaaagccatg ccggccttca
      721 ttgctgcgca aatcaaaaaa aaggagtaat atgttaaact atcaacaggt gctaccgttt
      781 attaagaaaa ctgatttgga agctttggaa ccccaagctc ttaaagctaa ggaagatttg
      841 cttcataaaa cttgtttggg caacgatttt gttggttggg ttgattaccc gaatcgctta
      901 agcgaagccg atttaaaagc aattgttgct gccagtcaaa cgattcaaaa tcaatctcaa
      961 gttctgctcg tcattggcat tggcggttcc taccttggag ctaagtcggc actgtcaatg
     1021 ttaagagctt attttaaaaa aaccggtggt cttgagattc tgtttgtggg gaatactttg
     1081 tcatcaactt ataccaaagc ggtgttacaa tatttacagc ataaagattt ttctatcaat
     1141 gtgatttcta aatcggggac aacgacggaa ccggcgattg catttcgttt gtttcgcggt
     1201 ctcttaaaag aaaaatatgg ttcccgtttt aatgaacgca tttattgcac gacaacgatt
     1261 ggtaaaggtg tcctttattc ggaagccatc aacaataatt atcagatttt tgctattcct
     1321 gaagatattg gcggtcgcta ttcggtttta actccggttg gattactacc gatggcttgc
     1381 agtaacatcg acatttacca agtcattaac ggggctagcc aagcgatttt taatttctca
     1441 caaaaaccgt tttgggaaaa ccaagttttg ctttatgcga gtttacgtaa agcgctgtat
     1501 gaccaaggaa aacggataga aatgctggtt tattatgaac caaaactgca ttattttggc
     1561 gaatggtata aacaattatt cggtgaatcc gaaggcaaaa accatttagg cttgttcccg
     1621 gtaacggcca attattcgac ggatctacac tcactgggtc agtatgttca agacggtcac
     1681 cgtcatttat tcgaaaccgt tttaaatatt aataaaccgg aagaggattt aacgattcct
     1741 ctagaccagc ttgattttga tcaattaaat tatttaacga aatattcgct ccaccaaatt
     1801 aatcatcaag ctttggtggg gacaacgatg gcccatgttg atgggggcgt tcccaatatt
     1861 ctcattaatg ttccggaact atc
//
LOCUS       DAOSGN010000181         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_62194_length_1882_cov_2.673235, whole genome shotgun sequence.
ACCESSION   DAOSGN010000181 DAOSGN010000000
VERSION     DAOSGN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62194_length_1882_cov_2.673235"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PK087_03035"
     CDS             <1..602
                     /locus_tag="PK087_03035"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOH18281.1"
                     /translation="DVSAENIRTITNCPTESPLINEIINNYPNEIAIANEVLGTAGQD
                     IYKEEAITWAATALAKQGDSQIGIINSGGIRSGAFPINNGSTVTYGHIFKMMPFENKV
                     VTLKMTGDELFNLFRSGLRFSKNVDTYNRTIDGIAIVGSQSYQVATIDFVYENYAGYF
                     SLGTNVVITDDLFRDCLAQEVKATVLALGKWYLQRPIND"
     gene            complement(718..1590)
                     /locus_tag="PK087_03040"
     CDS             complement(718..1590)
                     /locus_tag="PK087_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH18282.1"
                     /translation="MQNYVIITDATCDLPIWISKKFALEVIPMVYQMEGKDFQIDITN
                     PNFKLSEFYAKLRQGQMATTTQINAHQYEEVIEKYLAQGIDVLVINFSSALSGSFNSL
                     NIAKRTLDEKYPQRQLIIFDSKAASMGEGLLVYLALLEKQKGKTITEVAAWLSANYLH
                     LCHWFIVDDLDFLRRGGRLSGSIAFIGRLLKIKPILHVDNDGRLVSVKKAIGKNRALE
                     ELFQKLVETGINPNKQTIFISHGDALTDATNLKNLILARFPQAQIVIGEIGAIIGAHS
                     GPGTVALFFLGTER"
BASE COUNT          563 a          320 c          391 g          608 t
ORIGIN      
        1 ttgatgtcag tgctgaaaat attcgtacga ttaccaactg tccaaccgaa agcccgttga
       61 ttaatgagat tatcaacaat tatccgaatg aaattgccat tgctaatgaa gttttgggaa
      121 cggccgggca ggatatttat aaggaagaag ctattacttg ggcggctacg gctttagcca
      181 aacaggggga tagtcaaatc gggattatca attccggagg catccgttcg ggggcatttc
      241 ctatcaataa tggcagcacc gttacttacg gccatatttt taagatgatg ccgtttgaaa
      301 acaaagtcgt aaccctaaag atgacggggg atgaactatt taatttgttt aggtcgggat
      361 tacgctttag caaaaatgtt gatacttata accgcacgat tgatgggata gcgattgttg
      421 ggagtcaaag ttatcaagta gcaacgattg attttgttta tgaaaactat gccggttatt
      481 tttctttagg aacaaatgtc gttattaccg atgatttatt tagggattgt ttagctcaag
      541 aagtaaaagc gactgtccta gctttaggca agtggtattt acaaagaccg attaatgatt
      601 agtagtcata aaaaaagcat ttcgccatca aggtgaaatg cttttgttgt taataagagt
      661 ttatcttgac tttattttat attctcaata aaaaccgatt gctgttaagt tttttaatta
      721 tctttccgtt cctaaaaaga acaaagcgac ggttccgggt ccggaatgag cgccgataat
      781 ggcgccgatt tcaccaatta caatctgggc ttgcgggaag cgagctaaaa tgagattttt
      841 taaattggtg gcatcagtta aagcatcacc gtggctaatg aaaatagttt gtttattggg
      901 attgattcct gtttctacaa gcttttggaa taattcctcg agtgctctat ttttaccgat
      961 ggcttttttg acggaaacta aacgcccgtc gttatcaaca tgtaaaattg gtttgatttt
     1021 caataaacgc ccgatgaagg caatactgcc cgataagcgg cctccacgcc ttagaaaatc
     1081 aagatcatcg acgatgaacc aatggcaaag gtgtaaatag ttggcgctta accaagcggc
     1141 cacttcggtg atcgttttgc ctttttgttt ttccaaaagg gctaaataga ccaataagcc
     1201 ttcgcccatg gacgctgctt tggaatcaaa aatgattaat tggcgctgcg gatatttttc
     1261 gtccagcgtt cttttcgcaa tattcaaaga attgaaacta ccgcttaagg ctgaagaaaa
     1321 attaatcact aagacatcaa tcccttgagc aaggtatttt tcaatgactt cttcatattg
     1381 atgagcattg atttgcgtcg ttgttgccat ttgcccttga cgcaatttgg cataaaattc
     1441 gcttaactta aagttaggat tggtgatatc gatttggaag tcttttcctt ccatttgata
     1501 gaccatgggg attacttcta aagcaaattt cttgcttatc cagatgggaa gatcgcaggt
     1561 tgcatccgtg ataatgacat aattttgcat gttgaactcc ttttttttga ttataacgat
     1621 tttataaggc taatgcaaag taaatgtcgg gataataatt gtcaattaat gtaaatttgt
     1681 cacttttttt aagaaaacat ctaaatttaa tgggattgat gttataatta acaaaaagag
     1741 agatggagct ggcgtatgaa aaaatgtttt accattaata ctcttcgaaa tacgagtgat
     1801 tttcatagtt attatccctt gattcaagac cgcttatttg ctgctgttga gatattctat
     1861 cctgaacagc cggaaattag ag
//
LOCUS       DAOSGN010000182         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_62246_length_1881_cov_2.966046, whole genome shotgun sequence.
ACCESSION   DAOSGN010000182 DAOSGN010000000
VERSION     DAOSGN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62246_length_1881_cov_2.966046"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK087_03045"
     CDS             <1..227
                     /locus_tag="PK087_03045"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOH18283.1"
                     /translation="IIAWGFDTLGLKRIECTTFTNNLQSQRVCEKLGLVYEGLKRKGY
                     LLYDGSIGDLFCYSIIDDEFYERIRHGKWW"
     gene            230..970
                     /locus_tag="PK087_03050"
     CDS             230..970
                     /locus_tag="PK087_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOH18284.1"
                     /translation="MKKVFQLLILFLISFIVSGCTLVNQLFEPKIDTDTLNHISTVVL
                     NSNLKIKTTTYTKVFNTEIPGPLQGTGSGVIIKNTDQRYYLLTNYHVVVLSESYYHRY
                     TVEDIYNNVSSAVLLEYDQDYDLAILSSESTEEMEVMSLARSDPKVGELVFSIGSPSG
                     KHNIITAGEVLAYQNINNVSYEVIIHDAVIRHGSSGSMLINQNYEIVGLNTWGFDDEE
                     EITDYVIGGATPVSKIYEFLKAKGFIDE"
     gene            1051..>1881
                     /locus_tag="PK087_03055"
     CDS             1051..>1881
                     /locus_tag="PK087_03055"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOH18285.1"
                     /translation="MNKDNRQYLGPSLKDARKRLNKTVTKITFQTTEQLVGLGNNKKY
                     LIKTYGCQGNLADSEKMAGILELLGFTPTEKEEWADVIIINTCAVRENAENRVFGEIG
                     RLKSLKRHHPDLLLVLCGCMPQEERVVQMVMDKYPQVDIVFGTHNLHQLGEYLSTAYL
                     TKEKVVEVFSKEGDIVEDVPTKRAHNKKAWVNIMYGCDEFCTYCIVPYTRGKERSRLP
                     EDIFNEVKELIKEGYQEITLLGQNVNAYGHDFLNRQYRFADLLNDLNQMPIARVRFTT
                     S"
BASE COUNT          596 a          297 c          404 g          584 t
ORIGIN      
        1 aaatcattgc ttggggtttt gatactttgg gcttaaagcg aattgaatgc acgactttta
       61 caaacaacct tcaatcccag cgagtttgcg aaaaactggg cttggtttat gaaggcttaa
      121 agcgcaaggg ctatcttctt tatgatggtt ctattggcga tctcttttgt tattccatta
      181 ttgatgatga gttttatgaa cggattcgtc atggtaagtg gtggtgaaaa tgaaaaaagt
      241 ttttcaatta ttaattttgt ttctcattag ttttattgtt tctggttgca ctttagttaa
      301 tcaattattc gaaccgaaaa ttgacacgga tactttgaat cacatcagta ccgtggttct
      361 taattccaat ttaaaaatta aaaccacgac ttatacgaag gtcttcaata cggagattcc
      421 cggacctttg caaggaacgg gaagtggtgt catcatcaaa aacacggatc aaagatatta
      481 tttattgacg aattatcatg ttgtggtttt atcggaatca tattatcatc gttatacggt
      541 cgaagatatt tataataatg tttccagtgc ggttttgtta gaatatgatc aggattacga
      601 tttagcgatt ctcagctctg aatcaacgga agagatggaa gtgatgtcat tagcgaggag
      661 cgacccgaaa gtcggcgaat tggttttttc catcggcagt ccttcaggga aacacaatat
      721 tattactgcg ggtgaggttt tagcttacca aaacattaac aatgtcagtt atgaggtcat
      781 cattcatgat gccgtcatcc gtcatggcag tagtggtagt atgctcatta atcaaaatta
      841 tgaaattgtc ggtttaaata cttggggttt tgatgatgaa gaagaaatca ccgattatgt
      901 tatcggtgga gctaccccag tttccaaaat ttatgaattc ttaaaagcga aaggttttat
      961 tgatgaataa aagggatgag cccttttttc attaagggga gaatggtaaa ataataaaca
     1021 aggataataa cgaatactta ggtaaagaat atgaataaag ataatcgtca atatttaggc
     1081 ccatcattaa aagatgctcg taaacgctta aataaaacgg tcaccaaaat tacttttcaa
     1141 acgactgaac aattagtggg actgggtaat aataaaaaat acttaatcaa aacctatggt
     1201 tgtcaaggca atttggcgga cagcgaaaag atggccggga ttttggaatt gttggggttt
     1261 acaccgacgg aaaaagaaga atgggcggat gttatcatca tcaacacttg tgccgttcgc
     1321 gaaaatgcgg aaaaccgcgt gtttggggaa atcggtcgct tgaaaagttt aaaacgccac
     1381 catccggatt tgttgctggt gttgtgtggc tgtatgcccc aagaagaacg cgttgtgcaa
     1441 atggttatgg acaaataccc gcaagtcgat attgtttttg gaacgcataa ccttcatcaa
     1501 ttgggagaat atttatctac cgcttatttg actaaagaaa aggtagttga agttttttct
     1561 aaagaaggcg acattgtgga agatgttccc accaaaagag cccataataa aaaagcttgg
     1621 gtcaatatta tgtatggttg cgatgaattt tgcacttact gtattgttcc ctatacacga
     1681 ggaaaagaac gatcccgttt accggaagat atttttaatg aagttaagga actgattaaa
     1741 gaagggtatc aagaaatcac attattaggt caaaatgtca atgcttatgg ccatgatttt
     1801 ttaaatcgcc aatatcgctt tgcagattta ttaaatgatt taaaccaaat gccgattgcc
     1861 cgggtgcgtt ttactacttc c
//
LOCUS       DAOSGN010000183         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_62955_length_1868_cov_2.720905, whole genome shotgun sequence.
ACCESSION   DAOSGN010000183 DAOSGN010000000
VERSION     DAOSGN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62955_length_1868_cov_2.720905"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="PK087_03060"
     CDS             <1..1469
                     /locus_tag="PK087_03060"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HOH18286.1"
                     /translation="SVTFSQPEIILKINERSSLPLELQNISFDKLQFAFSKDNIAIIE
                     DESIKALNIGTTSLTVTVPSLSISTSIEVIVEGDEGEKSLAFFQEEIILKTNEGRSLP
                     LRLQNISFDELHFAFSKDNIASIENGLIKAQSIGCTTLTVTVPGLTITASLKVIVEAG
                     KVLSPVLYAPSNYLKLGMSTVILMKNEATLGASFDSFHWGLSNPDVISLNNNIITALK
                     HGEVSIIATLKSNPAIQASLTIKVGETSSIKDAHGEIGAGRMILSSPTIEAFIPAGES
                     IQLAIDGATNLDYYFWKSYDNQVAMAASGGKVFGVAPGVAEIAAFSPLSNDIYGTIKV
                     TVFGKPNVDYASRLVEVALNEEGYVEGYNNDTKYGDWWGIPNGEWCAMFVSWCANEAG
                     ISTVIIPKYAACTVGREWFEQRKQFQYKENYQPKKGDIIFFLSNGASHTGIVTGSDKT
                     YVYTIEGNTADRVAQRSYPLQHRTITGYGTPNYPPFSK"
     gene            complement(1509..>1868)
                     /locus_tag="PK087_03065"
     CDS             complement(1509..>1868)
                     /locus_tag="PK087_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18287.1"
                     /translation="SPIIFDKLFITLNIDDDPLDDVPVITSMSLFSYFYKTNYTGTQV
                     KFNSSMSGNYTVVIDLPTEYDFELYFPSSYVVVDGHGFYIANSIIPRKFEFTVTIIES
                     SSTDDWGQRLIVNYVIS"
BASE COUNT          578 a          403 c          384 g          503 t
ORIGIN      
        1 cttccgtcac tttttcccaa ccagaaatca tcttaaaaat caatgaacgc tctagcctcc
       61 ccttggaatt acaaaatatt agctttgata aacttcaatt tgctttttca aaggacaata
      121 ttgccatcat tgaagatgaa agtatcaaag ccctcaatat tgggactact agcttgacgg
      181 tcacggttcc cagtctttcg atatccacaa gcattgaggt gattgtggaa ggcgacgagg
      241 gagaaaaatc cctcgctttc tttcaagagg aaatcatctt aaaaaccaat gaaggacgta
      301 gcctcccttt gagattacaa aatattagtt ttgatgaact tcattttgct ttttcaaagg
      361 acaatattgc aagcatcgaa aacggactaa ttaaagcgca aagcattggt tgtaccacct
      421 taaccgtcac cgtccccggt cttaccatta cggcaagctt gaaggtgatt gtggaagcgg
      481 gcaaagtact aagccctgtg ctttatgccc caagcaatta tttaaagttg ggaatgtcca
      541 ctgtcatttt gatgaaaaac gaagcgacgc tcggagccag ctttgatagt tttcactggg
      601 gcttaagcaa tcccgatgtt atttccttaa ataacaatat cataactgcc cttaagcatg
      661 gcgaagtaag catcattgct actttaaaat ctaatcccgc tattcaagca agcttaacaa
      721 ttaaggttgg cgaaaccagt tctattaaag atgcccacgg cgaaattggg gcggggcgca
      781 tgattttgtc ctctcccact attgaagctt tcatcccggc cggagaaagc atccaattag
      841 caattgatgg tgctactaac ttagattatt atttttggaa gagttatgac aatcaagtgg
      901 ccatggccgc cagtggcgga aaagtcttcg gcgtggcccc gggggttgcg gaaatcgccg
      961 ctttctcacc actctccaat gatatctatg gtaccattaa agttaccgtc tttggaaaac
     1021 ccaatgttga ttatgccagt cgcttagtcg aagtcgcctt aaacgaagaa ggttatgtgg
     1081 aaggatataa taacgacacc aaatacggtg attggtgggg cattcccaat ggggaatggt
     1141 gtgcaatgtt tgtctcctgg tgtgccaatg aagccggaat ttccacggtc atcattccca
     1201 aatatgccgc ttgcaccgtc gggcgcgaat ggttcgaaca aagaaagcaa ttccaatata
     1261 aagaaaacta ccaacctaaa aaaggtgata tcatcttttt ccttagtaat ggtgcttccc
     1321 acaccggaat tgttaccggt agtgacaaaa cttatgtcta caccattgaa ggtaataccg
     1381 ccgaccgcgt tgcccaaaga agctaccccc tccaacatcg aacgattacc ggttatggca
     1441 cccccaacta tccacctttt tccaaataaa aaagatccca accgcaaaca agggttggga
     1501 ttattttatt aggaaataac ataattgacg attaatcgct gtccccaatc atccgttgaa
     1561 ctggattcaa taatcgttac ggtgaactca aatttccgtg gaataatcga gttggcaata
     1621 tagaatccgt gaccgtcaac gacgacatat gaggaaggaa agtataattc aaagtcatat
     1681 tccgtcggta aatcgatgac aacggtataa ttaccggaca tactactgtt aaacttaact
     1741 tgggttccgg tataattggt tttgtaaaag taggaaaaca aagacatgga agtaatgacc
     1801 ggaacatcat caaggggatc atcatcaata ttaagggtaa taaatagttt gtcgaaaata
     1861 atcggact
//
LOCUS       DAOSGN010000184         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_63217_length_1863_cov_2.706305, whole genome shotgun sequence.
ACCESSION   DAOSGN010000184 DAOSGN010000000
VERSION     DAOSGN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63217_length_1863_cov_2.706305"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PK087_03070"
     CDS             <1..818
                     /locus_tag="PK087_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOH18288.1"
                     /translation="AYYYQIKTAMKNKEFLLYYQPIINVDDGTLYGAEALLRWNHPEH
                     GVLSPHQFINIMEQSGDIYWVGLWGIENLIKQYFEIKKDFPELELRLSLNLSPKQLIV
                     LTLVADFLKILRKYRLQASNITLEIVEFALFERYDEIMVNIQKLKDVGFKIAIDGFGL
                     DFATLAKLEKMPIDIIKLDRSFLSDTQESYMTEKFATLLVGFARNNQKVVISEGVENY
                     EMLEKVSNFGINVVQGYYFGYPMSGDDLKSYIEGQSWKGKLLKEPEESVDKKK"
     regulatory      910..983
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      1059..1132
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1185..>1863
                     /locus_tag="PK087_03075"
     CDS             1185..>1863
                     /locus_tag="PK087_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18289.1"
                     /translation="MRLSKMLGVRKFAVRTTAVTIGLLITAFLSIAIAIITFYGQQAG
                     NFVMSIDPDSFTRGIILSSDDKFLAPFPRLLANPVIDVKDITYSWLKLSEIKATDGDY
                     IDEDYRYVAYTFYVKNIGSETVDVGYHLKITDTYKNVDEAVRILIIEDDTDEYLYQKP
                     DTVPYDYPKEMPKANYFIDDEIVTRGKIANLRPHMYKKYSVIMWLEGEDPDCVDSILG
                     GMIRIQMK"
BASE COUNT          585 a          288 c          388 g          602 t
ORIGIN      
        1 tggcttatta ttatcaaatt aaaacagcca tgaagaataa agagtttttg ctttattatc
       61 aacctattat caatgtcgat gacggaactt tatatggggc ggaagcctta ttaaggtgga
      121 atcatccgga acatggtgtt ttgtctcctc accaatttat caatatcatg gagcaatccg
      181 gggatattta ttgggttggt ttatggggca ttgaaaacct cattaaacaa tactttgaaa
      241 ttaaaaaaga ttttccggaa ctagaattac gcttatcctt aaacctgagc cctaaacaat
      301 tgattgtttt aactttggtt gctgatttct tgaagatttt acgcaaatac cgtttacaag
      361 cttccaatat cactttggaa attgtggagt ttgccttgtt tgagcgctat gatgaaatta
      421 tggtgaatat tcaaaaactt aaagatgtcg gttttaaaat tgctattgac ggctttggtc
      481 tggattttgc aactcttgcc aaactggaaa aaatgccgat tgacatcatt aaactggatc
      541 ggtctttctt aagcgatacg caagagtcct atatgacgga aaaatttgcc accctgcttg
      601 ttggttttgc tcgtaataac caaaaggttg ttatttctga aggagtggag aattacgaaa
      661 tgctggagaa agttagcaat tttggtatta atgtcgttca aggatattat tttggttatc
      721 cgatgtcagg tgatgatttg aaatcctata tcgaaggtca atcttggaag ggaaaacttt
      781 taaaagaacc ggaagaaagt gttgacaaaa agaaataacc cattatagtt gaactattta
      841 taattatttt tgtatttttg ttgacaagat tttattggtg tgttagaatg gaggcactta
      901 agatagaaaa atgttcaaag gcaaacttag agaaatctag ggacgcaaag ctaaagggcc
      961 tgaaatggta gccagttgtc attgtaagaa tgatactggc ttttttcttt ctttaaggat
     1021 ataatttaat aagttttatt tatcttttta tcaagaagat atgtaaaagg caaacttaga
     1081 gaaatctagg gacgcaaagc taaagggcct gaaatggtag ccagttgtca ttattagaat
     1141 gatactggct attttttatt tccacaaaga aaggcggtgt ttgaatgcgg ctttccaaaa
     1201 tgcttggagt tagaaaattt gctgtccgaa caacagctgt gaccatcggt ttgctgatta
     1261 cggcgtttct atcaatagcc attgccatca tcaccttcta tggtcaacaa gccggaaact
     1321 tcgttatgtc gattgacccc gattcattca cccgtggcat catcctttct agtgacgata
     1381 aattcttagc tcctttccca agattgttag ctaatcccgt tatcgatgtg aaagatatta
     1441 cttattcctg gttaaagcta tcggagatta aagcaacgga tggcgattat atcgatgaag
     1501 attatcgtta tgttgcctat accttctatg tcaagaacat tggcagtgaa accgtggatg
     1561 ttggttatca tttaaaaata actgatactt acaaaaatgt tgatgaagcc gttagaatat
     1621 taattatcga agatgatacc gatgagtatt tgtatcaaaa gcctgatacg gttccctatg
     1681 attacccgaa agagatgccg aaggctaatt acttcatcga tgatgaaatt gtgacgagag
     1741 gaaagattgc caacctccga ccccacatgt ataaaaaata tagtgtaatt atgtggttgg
     1801 aaggtgaaga tccggattgt gtcgattcaa tattaggggg gatgattagg attcagatga
     1861 aat
//
LOCUS       DAOSGN010000185         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_63371_length_1860_cov_2.850970, whole genome shotgun sequence.
ACCESSION   DAOSGN010000185 DAOSGN010000000
VERSION     DAOSGN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63371_length_1860_cov_2.850970"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..1499)
                     /locus_tag="PK087_03080"
     CDS             complement(288..1499)
                     /locus_tag="PK087_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH18290.1"
                     /translation="MEQQQIKRGIINIIFLGLVSFFIDLSTEMVYPLVTLYLQTFASV
                     PIVGVIEGFAESTAALLKVYSGYLGDKYQNKKTLAFFGYSSAIAYKNILFFSGSWVGI
                     MIARLVDRFGKGIRTAPRDALVAESGEAALGKSFGLHKMLDMLGAGLGILIAYLLYQQ
                     LVKDNMLIDIKAFKTIFLLSIIPAVLGLVFLSLVRERKKNTKWGNRFTLKGLKLNPRL
                     KWYLVVILLFSLGNSSNAFLLLKATDAGYTTSFVLLLYLVYHLVSTIFAFPMGKWSDR
                     IGRHKLVVPGYLLYGFLYFGFAFLTSKVSLMVLFGAYGLYQAIISGAEKAFITEFAPK
                     DAKGTIIGLYGTCQGVGILVASLLAGGLWTWLGARAPFVFASIMGLAAAVLAFLVMTH
                     QFQDSNSLKKV"
     gene            1727..>1860
                     /locus_tag="PK087_03085"
     CDS             1727..>1860
                     /locus_tag="PK087_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HOH18291.1"
                     /translation="MKYQIEGGSLPVLIVNLEVGEKLITEGGAMSWMSGNMKMDTSSG
                     G"
BASE COUNT          647 a          392 c          317 g          504 t
ORIGIN      
        1 aattctgtac agtattcaac aacaccttcc caagatgctt ggttgaatga tctgtcatca
       61 atatcacctt tatcggtgac taaagcgact gtgaaagttt cctttccagt acagccgatt
      121 aacgaaaaaa cagtaagggc caataagaat gctaataaaa acttcttcat tattcctcct
      181 caaaaaaagt tttatctatg tagtaaatag ttaactactt tttcattgta acaaacaaag
      241 cccaaaaagt caattatgaa atatgctgat ttcttaagat tttgctttta aacttttttt
      301 aggctgttgc tgtcttggaa ttgatgagtc ataaccaaga aagccaaaac cgctgccgcc
      361 aaacccatga tgcttgcaaa aacaaatggc gctcttgccc ccaaccaagt ccataaacca
      421 ccggctaaaa gcgaagccac caagattccc actccttgac aagttccata taaaccaatg
      481 atggttcctt ttgcatcttt cggagcaaac tcggtaataa aggctttttc cgcccccgaa
      541 ataatggctt gataaagtcc ataagcacca aataaaacca ttaacgaaac tttgcttgtc
      601 aagaaagcaa aaccaaaata caaaaaacca tataataaat aaccgggaac aactagttta
      661 tgacgaccaa tccggtcgga ccattttccc atcggaaaag caaagattgt tgaaacgaga
      721 tgataaacta agtaaaggag taaaacaaaa ctggtagtat aaccggcatc cgtcgccttt
      781 aacaacaaaa aagcattgct agaattacca agactaaata acaagatgac cactaaatac
      841 catttaagac gggggtttaa tttaagtccc tttagggtga agcgattgcc ccatttggtg
      901 tttttctttc gttctctcac aagcgataag aaaactaaac ccaaaaccgc cggaataatt
      961 gatagtaaaa agatggtttt aaaggcttta atatcaatga gcatattatc ttttactaat
     1021 tgctggtata aaagataagc aatcaaaatc cccaaaccgg cgcccaacat atcgagcatt
     1081 ttgtgaagcc caaaggattt cccgagcgct gcttcccccg attccgccac caaagcatca
     1141 cgaggtgctg ttctaatgcc tttcccgaaa cggtccacaa gccgagcaat catgatgcct
     1201 acccagcttc cggaaaaaaa caaaatgttt ttataagcaa tggcactgga ataaccgaaa
     1261 aaagccaatg tctttttatt ttggtattta tcaccaagat aaccggaata gactttcaaa
     1321 agggcggccg tgctttccgc aaacccctca ataacaccaa caatgggaac ggaagcaaag
     1381 gtttgtaaat aaagagttac tagcgggtag accatttccg tagacaaatc aataaaaaaa
     1441 ctgaccaaac ctaaaaagat gatattaatg attccgcgtt taatctgttg ttgttccata
     1501 gcttcacctt tatcatcaat gattatacgc tttttttctc cattttcaat ataaagaagc
     1561 gaagcttacc aaagagcatt atttcctaaa accaatatca atcacatcag atatgttttc
     1621 ctcctttttt aaaaaagaca ttatttgaca cgaccttgct ttcttattat aatatagtca
     1681 tgccatagtt ttttccataa aatagctgtg gccaaaagga gttaacatga aatatcaaat
     1741 tgaaggtggc agcttgccgg ttttaatcgt taatttggaa gtcggtgaaa aattaatcac
     1801 tgaaggtgga gcgatgtcgt ggatgagcgg caatatgaaa atggatacca gctccggggg
//
LOCUS       DAOSGN010000186         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_63743_length_1852_cov_2.104619, whole genome shotgun sequence.
ACCESSION   DAOSGN010000186 DAOSGN010000000
VERSION     DAOSGN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63743_length_1852_cov_2.104619"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PK087_03090"
     CDS             <1..1263
                     /locus_tag="PK087_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOH18292.1"
                     /translation="STSGEVNLSKAQEEVLLTLRLVKYDEVYEQTFRVVTAIRPYTVN
                     DYEYFVNQLKLDVQALIMDEAEINFFNHQVLSTPATKTVNLETIAETQSKTSIYNLIT
                     SYNHFTKYPIYQPSGALATDSEKNSILENRNLNGLADEIAIQYAVSTTHVNLRSYPTD
                     FYSSNYSTERFQETGFGLGIPMVIYHTSLDGQWFFVQMLNYYGWVRAKQVAFCTREQF
                     LSYVNPEQFVVVIDSDFVLGEEYLRMGTRLPYFSKTDKDYLLAFPVRSCLGFLQIVDF
                     SFTNQGELSDGYLPYNYENLLSQAFKMLGVSYSWGDKQVKGFDCSSTQAAIYQCFGFF
                     LGRNTSNQRVTKQYGGTLSNLSNESLKDMKVGTLLYTSGHVLMFIGVDEAGQCWLLHN
                     TSLGNKTILQTLNDYGTTNIKYYLSFHN"
     gene            1391..>1852
                     /locus_tag="PK087_03095"
     CDS             1391..>1852
                     /locus_tag="PK087_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOH18293.1"
                     /translation="MDKFFIFSTIGIAVIAFVFAFWLYIWVSKQPSSNKKVASIGELI
                     RNGANVFLRKEYLVLAKFSGVIAILIFIFLPEPIWKGKILENLTMFIAYLFGTGLSAL
                     AGKIGIQVATIANVKTAEAAQKGIKPSFLSGFRGGAVMGMAVVGASLLGVAL"
BASE COUNT          541 a          316 c          380 g          615 t
ORIGIN      
        1 tccaccagtg gggaagtaaa cttaagcaaa gcccaagaag aagtcctttt aacattaaga
       61 cttgtgaaat acgatgaggt ttatgaacaa acatttaggg ttgtaacagc tattcgtcct
      121 tataccgtca atgattatga atattttgtc aatcaactta agcttgatgt tcaagctctg
      181 attatggatg aagcggaaat taactttttc aaccatcaag ttttaagtac gccagcaaca
      241 aaaaccgtta atttggagac gattgcggaa acgcaaagca agacaagcat ttataattta
      301 atcaccagtt ataatcattt tactaaatac cccatctatc aaccatcggg ggcattagct
      361 accgatagtg aaaaaaacag cattttggaa aatcgtaatt taaatggttt agcggatgaa
      421 atcgccattc agtatgccgt ctcaacgacc catgtcaatt tacgaagcta tccaacggac
      481 ttttattcat cgaattattc aaccgaacgc tttcaagaaa ctggttttgg tttaggcatc
      541 ccgatggtta tttatcatac tagtttggac gggcagtggt tttttgtaca gatgcttaat
      601 tattatggtt gggtgcgggc caagcaagtt gctttttgta caagagaaca atttctaagc
      661 tatgttaatc cggagcaatt cgtcgttgtt atcgatagtg attttgtcct tggggaagaa
      721 tatcttcgca tgggcacgcg gttaccgtat ttttccaaaa cggataagga ttatttgctt
      781 gcctttcctg ttcgtagttg cttaggattt ttgcagattg ttgattttag ttttaccaat
      841 caaggtgaat taagtgatgg ttatcttcct tataattatg aaaacttatt atcccaagct
      901 tttaaaatgt tgggagttag ttacagttgg ggggacaaac aagttaaggg ttttgactgc
      961 tcttccacgc aagcggctat ttatcaatgc tttgggtttt tcttggggcg caacacttcc
     1021 aaccaacggg taactaaaca atatggcggc accttaagca atttaagcaa tgaatcatta
     1081 aaagacatga aggttggaac acttctttat accagcggtc atgtattaat gtttattggt
     1141 gtcgatgagg ctggtcaatg ctggttatta cataatactt cgcttggtaa taaaaccatc
     1201 cttcaaactt tgaatgatta tgggacaacg aatatcaagt attacttatc ttttcataat
     1261 taattgtaag ctctttaaac ggagcttttt ttaccttttc ataataaaat ttgtttttgc
     1321 atgcgaccat tgaaaacgct tggaaattgt gatagaataa cattagtttt cacaaaaagg
     1381 agtttaatga atggacaaat tttttatttt tagtacaatc ggtatcgctg tcattgcctt
     1441 tgtctttgct ttttggctgt atatttgggt ttccaaacaa ccatcaagca ataagaaagt
     1501 tgctagcatt ggtgaactta ttagaaacgg cgccaatgtc tttttaagaa aagaatatct
     1561 cgttctggct aaattttctg gcgtaatcgc tattttaatc tttatctttt tgccggaacc
     1621 gatttggaag ggaaaaattc ttgagaactt aaccatgttt attgcttatc tatttggcac
     1681 cgggctaagt gctttggccg gtaaaatcgg gattcaagtt gcgactattg cgaatgtgaa
     1741 aactgctgaa gccgcacaaa aaggaattaa gcctagcttc ttaagtggat tccgtggtgg
     1801 agcggtcatg ggaatggccg ttgtcggtgc ctcattgtta ggtgtggccc ta
//
LOCUS       DAOSGN010000187         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_63895_length_1849_cov_2.517280, whole genome shotgun sequence.
ACCESSION   DAOSGN010000187 DAOSGN010000000
VERSION     DAOSGN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63895_length_1849_cov_2.517280"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK087_03100"
     CDS             complement(<1..369)
                     /locus_tag="PK087_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOH18294.1"
                     /translation="MLELLKNDVIYYVISAVLALLVMFGIFLMSKVDKARLGNGISAF
                     SLLLAVIITLLRHEILGIWFLYIFLFVGAVIGIFMAVKVKMIETPQMIALLNGLGGLA
                     STIVGGFALFKIGAEDNLFSQ"
     gene            complement(381..668)
                     /locus_tag="PK087_03105"
     CDS             complement(381..668)
                     /locus_tag="PK087_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOH18295.1"
                     /translation="MEWILVAVFLVSTILGYILINNVPSLLHTPLMSGMNALSGITLI
                     GALIATAISLKDAHGVLASILGGIAIIVATINVFGGFGVTDRMLKMFKKKG"
     gene            complement(668..1831)
                     /locus_tag="PK087_03110"
     CDS             complement(668..1831)
                     /locus_tag="PK087_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOH18296.1"
                     /translation="MIIGIPKEILHGESRVSATPETVKKMVQDGHQVLVEVNAGENAF
                     YHDNDYQAAGATMLSCPKDIFQKADIILKVKEPQYNENLKEHEIDWMREGQTLITFLH
                     PAAPNNHEMIKRLTAKGVKALTLDGIPRISRAQSMDALSSMSTCAGYKGILIAADSLG
                     KFIPMIGSAVGMIKPANVLVVGTGVAGLRAIATARGLGAVVYATDIRPEANEQAQSLG
                     AKIVESGIPSEIAVSSDGKHANKLSEKWLAVERDHLREIVAKADIIFLSALVFGKRAP
                     IIIDEEMVKSMKPGSYIIDISIDQGGNCAVTKAGEVVNYFGIQVDGTKNIPGMLPTSS
                     TWMFAQNIYNLLKYLVKDNEVVLDLSDEITSSILVCHDYKLVHEGTLEAMGLK"
BASE COUNT          595 a          411 c          312 g          531 t
ORIGIN      
        1 ttggctaaac agattatcct cagcacctat tttaaatagc gcaaatcccc cgactatggt
       61 cgaagctaaa ccacctaaac cattaagcag agcaatcatt tgcggtgttt caatcatttt
      121 gactttaaca gccatgaaaa taccaattac ggcaccaaca aataaaaaga tatacagaaa
      181 ccaaatacca agaatctcat ggcgaagtaa agtaatgatg accgcaagca acaaactgaa
      241 agcactgatg ccattaccca aacgggcttt gtcaacttta ctcattaaga aaatgccaaa
      301 catgactaat aaggctaaga cagcggaaat aacataataa attacatcat tttttaataa
      361 ttctaacatt ctatttaacc ttatcctttt ttcttgaaca ttttgagcat ccgatcagtg
      421 acaccaaagc cgccaaagac attaatggta gccacaatga tggcaatgcc acccaatatt
      481 gatgctagca caccatgtgc atctttcaaa gaaatggctg ttgcgattag tgcgccaatc
      541 aaagtaatcc ccgaaagtgc attcattcca ctcattaagg gcgtatgtaa caaagagggg
      601 acattattaa tcagtatata acctaaaatg gtagaaacta aaaaaacagc aactaaaatc
      661 cactccatta tttcaacccc atggcttcta gtgttccttc gtgaacaagt ttataatcat
      721 ggcagacgag aatcgatgag gtgatttcat cactcaaatc aagaactact tcattgtctt
      781 tcacaagata ttttaataaa ttgtagatgt tttgtgcaaa catccaagtt gacgaagtcg
      841 gtaacatccc cggaatattt tttgttccat caacttgaat gccaaaataa ttgactactt
      901 cgccagcttt ggttaccgca caatttccgc cttgatcaat agaaatatca attatatagg
      961 atcccggttt catggatttg accatttctt catcaataat aatcggcgct cttttaccaa
     1021 atactaaggc agaaaggaaa ataatatccg ccttggcaac gatttcgcga agatgatcac
     1081 gttcaacagc gagccacttt tcacttaatt tattagcgtg tttgccatca ctgctgacgg
     1141 caatttcgct cggaatgccg gattcaacaa ttttagctcc taaagattga gcttgttcat
     1201 tggcttcagg acgaatatca gtggcataaa caacggctcc caaaccccga gctgtcgcaa
     1261 tagctcgtaa tccagcaaca ccggtgccaa caactaaaac attagcaggc ttaatcattc
     1321 caacagctga cccaatcatc gggatgaatt tccctaaact atcggcagcg attaaaatac
     1381 ctttatagcc agcacaagta ctcatggatg ataaagcatc catcgattgc gctcgactga
     1441 ttcgtggaat accatcaagc gttaaggctt taacaccttt ggccgtcagt cgtttaatca
     1501 tttcatgatt gttgggagcg gcggggtgta aaaaagtaat tagggtttgc ccttccctca
     1561 tccaatcaat ttcatgttct tttaaatttt cattgtattg tggttcctta acttttaaaa
     1621 taatatccgc tttttgaaaa atgtctttcg gacaactaag catggttgcc ccagcagctt
     1681 gatagtcatt gtcatgataa aaagcatttt cgccagcgtt aacttcaact aagacttgat
     1741 gtccgtcttg gaccattttt ttaactgttt cgggagtcgc ggacactcga gactcaccat
     1801 gtagtatttc ctttggaata ccaataatca taaaaaatcg cctcttttc
//
LOCUS       DAOSGN010000188         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_64777_length_1832_cov_3.487338, whole genome shotgun sequence.
ACCESSION   DAOSGN010000188 DAOSGN010000000
VERSION     DAOSGN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64777_length_1832_cov_3.487338"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PK087_03115"
     CDS             <1..289
                     /locus_tag="PK087_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18297.1"
                     /translation="QHYDYSQKECPQTLRRNNLYPNAIELIKAEYEVLTQLAGYTITF
                     QSNNPEYVNHYGRIINLPSVSTPITYTVRITNSQGYDATVMLISILPAKPA"
     gene            439..1521
                     /gene="nusA"
                     /locus_tag="PK087_03120"
     CDS             439..1521
                     /gene="nusA"
                     /locus_tag="PK087_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOH18298.1"
                     /translation="MVSQKFFESLEAIAFERGLDVEDVKGKVEIAISVSSRNSGYSGD
                     IKTEWDFEKKRIRVYEYKYVVETIDPDGPRGQITLEEAQAIKPKTKVGTEFKTEINVS
                     GFGRKAASVFRQSLLSGLKELEREEAFNFFQERVGEVITATVLDYSNNFVTFSIGKNV
                     VANMPDKEGVPGETFNVGDEKKVYITKVEKTTKGPKVFLTRANREIVKRLFEMMIPEI
                     ADGSIEIMGIAREPGEKTKIGVLSLKNNLDAKGACVGQGGLRIRQINEILNNEKIEIF
                     TWRDNPVDLIAEALLPAKALAVMVDEKTKQATVVVNDDQYSLAIGKSGQNARLAAYAI
                     GWRIDIKNLTDATNEGIEFEYNVSRK"
     gene            1549..1791
                     /locus_tag="PK087_03125"
     CDS             1549..1791
                     /locus_tag="PK087_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOH18299.1"
                     /translation="MRKCVVTNEQHPKGEMFRIVRTAEGPTVVDLTGKVRGRGAYLSK
                     NKEVILMAMKKKTLDRHLETKVPDEVFEQLLKILGE"
BASE COUNT          601 a          318 c          425 g          488 t
ORIGIN      
        1 acagcattat gactatagcc aaaaagaatg cccccaaact ttgcgtcgta acaatcttta
       61 ccctaatgcc attgaactaa ttaaggcaga gtatgaagtt ctaactcaat tggccggtta
      121 caccatcacc ttccaatcca ataacccgga atatgttaat cattatggtc ggattatcaa
      181 tttgccgtcg gtcagcacgc cgattactta cacggtgcgg attactaata gtcagggtta
      241 tgatgctacg gtgatgttga tttccattct tccggctaaa ccggcttaaa gctaaacaat
      301 taattgacaa aaataacaag cttgtgatac aatatggcat agaatgtcgt taactaagga
      361 gtgagtcgtc tcactccttt aaataataat gcacccgttt tgggaaatat gtcgaatgga
      421 caaaatcttg gaggaaacat ggttagtcaa aagttttttg aatcactgga agccattgct
      481 tttgagcgtg gtttggatgt tgaggatgtt aaagggaaag tggaaattgc gatttccgtc
      541 tccagccgta acagtggcta cagtggtgat attaaaaccg aatgggattt tgaaaagaaa
      601 agaatccgcg tttacgaata taaatatgtt gtcgagacca tcgatccgga cggaccacga
      661 gggcaaatca ctttagaaga agcgcaagcg attaaaccga aaacgaaagt gggaacggaa
      721 tttaaaacgg aaattaatgt ttccggtttt gggcggaaag cagcttctgt ttttcgtcaa
      781 agcttattaa gcggactgaa agaattagaa cgtgaagaag catttaattt tttccaagaa
      841 cgtgtcggcg aagtcatcac ggcgacggtt ttagattaca gcaataattt tgttactttc
      901 agtattggta aaaatgtcgt tgccaatatg cccgataaag aaggcgttcc cggggaaacc
      961 ttcaatgttg gcgatgagaa aaaagtttat attaccaaag tagaaaaaac caccaaagga
     1021 cctaaggttt tcttaacacg tgctaatcgg gaaattgtta agcgtttgtt tgaaatgatg
     1081 attccggaaa ttgccgacgg ttccattgaa atcatgggga ttgcgcggga accgggtgaa
     1141 aagaccaaaa tcggggttct gtctttaaaa aacaaccttg atgctaaagg ggcctgtgtt
     1201 ggtcaaggcg ggttaagaat tcgccaaatt aatgaaatct tgaataacga aaaaattgaa
     1261 atctttactt ggcgggataa tccggttgat ttgattgcgg aggccttgct tccggctaaa
     1321 gccttagccg ttatggttga tgaaaaaacc aaacaagcga ccgtcgttgt taatgatgac
     1381 caatactcac ttgccatcgg caagagcgga caaaatgccc gtttggcggc ctatgccatc
     1441 ggatggcgaa tagatattaa aaatttgacc gatgccacga atgaaggaat tgagtttgaa
     1501 tataacgttt ccagaaagta ggcgacaatg aaaccaagaa agataccgat gcgtaaatgt
     1561 gttgtgacta atgaacaaca tccaaaaggc gaaatgtttc gcattgttcg gacagcggaa
     1621 ggaccaacgg tggttgattt aaccgggaaa gtccgagggc gtggcgctta tttatccaaa
     1681 aataaagaag tgattttaat ggccatgaaa aagaaaacct tggatcgtca tttggaaaca
     1741 aaagtgcctg atgaggtttt tgaacaatta ttaaagattc taggtgagta atttgcaaga
     1801 cagtcaaaaa aataaaatca tgaatttatt gg
//
LOCUS       DAOSGN010000189         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_65033_length_1828_cov_2.439932, whole genome shotgun sequence.
ACCESSION   DAOSGN010000189 DAOSGN010000000
VERSION     DAOSGN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65033_length_1828_cov_2.439932"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="thyA"
                     /locus_tag="PK087_03130"
     CDS             <1..636
                     /gene="thyA"
                     /locus_tag="PK087_03130"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011283541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOH18300.1"
                     /translation="EWPFQKYQLSKEYQQESMEEFVDKIKQDSAFAAKWGELGPVYGH
                     QWRHFTSQTREVDQLATVINEIKTNPNSRRLIVNSWQAADLGEMALPPCHLLFQFYVN
                     KQQLSCQLYQRSADAFLGVPFNIASYSLLTMMVAKVCGLEVGSFVHTIGDAHIYANHL
                     EQVALQLRREPKALPKMMIKRDVRSIDDFVFEDFELLDYHPHPTIKGKVAV"
     gene            633..1112
                     /locus_tag="PK087_03135"
     CDS             633..1112
                     /locus_tag="PK087_03135"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOH18301.1"
                     /translation="MISIIVAISQNGVIGKGKELPWNYPEDLRYFKQVTSYHTVVMGR
                     TTFEGIVSRLHQPLPNRHNVVVSKNPNWHYPNVTVVHDYQKFLKTPHPDEVFIIGGRN
                     IYQEALPFAKRLYITHIHRHYEGDIYFPEVDFSQFRLIKETFCDEISFCVYERIDSN"
     gene            1090..>1828
                     /locus_tag="PK087_03140"
     CDS             1090..>1828
                     /locus_tag="PK087_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOH18302.1"
                     /translation="MKGLTVINMIILIISLMAILASSLYMYFTQYLTTGIIWWSFPVL
                     FLVFWLLSFLLFYGILAIMTLFMNPKKVVVHPYKFYSLMIAWTTRFLATFYRLDIVFE
                     NDHLLPHNSKFFLISNHQSNLDPIALLAVMKKHTVTFIMKDSLLNLPVIGPWLRTSGY
                     LPLDRKNDRKALEVVALAIKRIKDGKALGVYPEGTRSRGPKMNEFHHGIFRVPQKAEA
                     PIVVVAIDNLYRVKKRFLWTRTKVLIRI"
BASE COUNT          504 a          351 c          371 g          602 t
ORIGIN      
        1 gaatggcctt ttcagaaata tcagctttct aaagaatatc aacaagaaag catggaagaa
       61 tttgttgata agattaaaca agattccgcc tttgcggcta aatggggaga gctcgggcct
      121 gtttatgggc atcagtggcg ccattttaca agccaaactc gggaagttga ccaattagcg
      181 accgttatca atgagattaa aacaaacccc aattcacgaa gactaattgt taacagttgg
      241 caagcggctg atttagggga aatggcttta ccaccctgcc atttgttgtt tcagttctat
      301 gttaataagc agcaattgtc ctgtcagctt tatcaacgca gtgccgatgc ctttttaggg
      361 gtgccgttta atattgcttc ctactcctta ttaacaatga tggtagcgaa ggtttgtggt
      421 ttggaagtag gaagctttgt tcataccatt ggtgatgcgc atatttatgc caatcatctt
      481 gaacaagtcg ctttacagtt aaggcgtgaa cctaaagctc tgcctaaaat gatgattaaa
      541 cgcgatgttc ggtcaattga tgattttgtg tttgaagatt ttgaattgct tgattatcat
      601 ccgcatccga ccatcaaagg aaaggtggct gtatgatttc aattattgtg gccattagtc
      661 aaaatggcgt catcggtaaa ggcaaagaat tgccttggaa ttatccggaa gatttacgct
      721 atttcaaaca agtaacctcc tatcataccg tagtcatggg acggaccacc tttgagggca
      781 ttgtttcccg tttacatcaa ccattaccca atcgccataa tgtcgtagta agcaagaatc
      841 ccaattggca ttatcccaat gttaccgtcg tccacgatta tcaaaagttt ttaaaaacgc
      901 cccaccccga tgaagttttt atcattggtg gacggaatat ttatcaagaa gcccttcctt
      961 ttgctaagcg gctttacatt acccatatcc accgtcatta tgaaggagat atctattttc
     1021 ccgaggttga tttttcgcaa tttcgcttga ttaaagaaac attttgtgat gaaatctctt
     1081 tttgtgttta tgaaaggatt gacagtaatt aatatgatta tcttgattat ttcattaatg
     1141 gcgattctgg cttcttcttt atatatgtac tttactcaat acttaacaac ggggatcatc
     1201 tggtggagtt ttcccgtttt gtttcttgtt ttttggcttc tctcctttct tttgttctat
     1261 ggtatattag caatcatgac cttgtttatg aatcccaaaa aagtagttgt tcatccctat
     1321 aagttctata gtctaatgat tgcgtggacg acccgctttt tagcaacttt ctatcgtttg
     1381 gatattgttt ttgaaaatga ccaccttctt cctcacaaca gtaagttttt tctcatttcc
     1441 aatcatcaat cgaatttaga tccgattgcc ttattggcag ttatgaaaaa acatacggtt
     1501 acttttatta tgaaagatag tttattaaat ttgccggtga ttgggccgtg gttacgcact
     1561 tcgggctatc tgcccttgga tcgcaaaaat gaccggaaag ccttagaagt ggtggccctt
     1621 gccattaaac ggattaagga tggtaaagct ttgggagttt acccggaagg aacaagaagt
     1681 cgaggaccca agatgaatga attccatcat ggcattttcc gcgttcccca aaaagcggaa
     1741 gcaccgattg tggtcgtggc tattgataat ctttatcgtg tcaaaaaacg gtttttatgg
     1801 acgcggacaa aagttttaat tcgcattt
//
LOCUS       DAOSGN010000190         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_65354_length_1822_cov_3.172609, whole genome shotgun sequence.
ACCESSION   DAOSGN010000190 DAOSGN010000000
VERSION     DAOSGN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65354_length_1822_cov_3.172609"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..535
                     /gene="rpsJ"
                     /locus_tag="PK087_03145"
     CDS             230..535
                     /gene="rpsJ"
                     /locus_tag="PK087_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOH18303.1"
                     /translation="MAKQALRIRLVSYDHRLLDQSAKKIVDAAKKTGAVVSGPIPLPT
                     EKEVFTIIRSPHKYKDSREQFERRTHKRLIDIVETTPKTMDALIHLEIPAGVDIILK"
     gene            560..1219
                     /gene="rplC"
                     /locus_tag="PK087_03150"
     CDS             560..1219
                     /gene="rplC"
                     /locus_tag="PK087_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007203551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOH18304.1"
                     /translation="MAKGILGKKVGMTQVFSLEGTLIPVTVIEVTPNVVLQKKTKETD
                     GYQSVQLGYSDKPQRLATKPEIGHASKANANPKRFIKEIAGEEMNFFEVGQEVKANIF
                     KEGEIVDVAGTSKGKGYSGAIKRHNLKRGQMAHGSGYHRGAGSMGERTALRIKKGKKM
                     AGRMGGEQVTIQNLEIVKIDLEKNIMLIKGNVPGANRSYVVVTNGVKARTPEVNPEAL
                     A"
     gene            1234..>1822
                     /gene="rplD"
                     /locus_tag="PK087_03155"
     CDS             1234..>1822
                     /gene="rplD"
                     /locus_tag="PK087_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOH18305.1"
                     /translation="MPSIEVYNQLGEKIGKTNLKKNVFAIEPNMQVVYDVVNAERAAM
                     RQGTSKTKTRTEVRGGGRKPWRQKGTGRSRQGSIRAPQWVGGGVVFGPTPRKYNVKVN
                     RKVVQLAVKSLLSDRFKKKNIIVLDKLELADFRTKSFVAVLNNLKIVGKTIFIIPEED
                     FNLFMASRNIPNVYVQTKQHLSVYDLINADTYVTTE"
BASE COUNT          612 a          351 c          402 g          457 t
ORIGIN      
        1 tatcataaaa tttttttcta ctttctattt acaatctacc tgccaagaga aaaaacatct
       61 tttccttgac attaagaact gtttttggta atatatacaa ggctttgaaa gaggcagtga
      121 tatgcgaggt tgctgacact cacggttgcg ttgccaatgc cgttattcgt caggtaattc
      181 gtatggagcc aagctacatt ttaaatggat gcaaaaggag gaccaaaaaa tggcaaaaca
      241 agcattaaga atcagattag tttcttatga tcatcgattg ttagatcaat cagcaaaaaa
      301 aatcgttgat gcggctaaaa agaccggagc agttgtttcg ggaccgattc cgcttccaac
      361 ggaaaaagag gtattcacga ttattcgttc accgcacaag tacaaagaca gtcgggaaca
      421 atttgagcga agaactcaca aacgtttaat cgacatcgtc gaaaccactc cgaaaacaat
      481 ggatgcctta atccatttgg agatccccgc cggtgtggat attatcttaa aataatgagt
      541 aaagtaggag gtgaaactca tggccaaagg aatcttaggt aaaaaagtcg gaatgacaca
      601 agttttctcc ctagaaggaa ctttaatccc cgttacggta attgaagtta ctcccaatgt
      661 ggtattacag aagaaaacaa aagaaacaga tggttatcaa tctgttcaac ttggttatag
      721 cgacaagcca caacgacttg ccactaaacc ggaaatcgga catgcctcaa aggcaaatgc
      781 caaccctaag cgcttcatca aagaaatcgc tggggaggaa atgaatttct ttgaggtcgg
      841 tcaagaggtt aaagcaaata tattcaagga aggcgaaatt gttgatgtcg ccggaaccag
      901 caagggaaaa ggttattcag gcgccatcaa acgccacaat cttaaaagag gccaaatggc
      961 acatggctcc ggttaccatc gcggtgctgg ttccatgggc gaacggaccg ctcttcgtat
     1021 taaaaaaggt aagaaaatgg ccggccgcat gggtggtgaa caagttacca tccaaaattt
     1081 agaaatcgtt aaaatcgatt tagaaaaaaa tattatgtta ataaaaggta acgttcccgg
     1141 agcaaatcgc tcttatgtcg ttgttactaa cggtgtaaaa gcgcgcactc ccgaagttaa
     1201 tcctgaagca ttagcgtagg ataggagaaa cagatgccaa gtattgaagt atataatcaa
     1261 ttgggtgaaa aaatcggcaa aaccaatctt aaaaagaatg ttttcgcaat tgaacccaac
     1321 atgcaagtgg tctatgatgt tgtcaacgcc gaacgagctg ccatgcgcca aggaaccagc
     1381 aaaaccaaaa cccgcacgga agtaagaggc ggaggtcgta aaccgtggcg tcaaaaaggc
     1441 accggtcgtt cccgtcaagg tagcattcgt gctccgcaat gggttggcgg tggggtcgtt
     1501 tttggtccaa cgccaaggaa atataatgtg aaagtcaatc gcaaagtggt gcaattggct
     1561 gttaaatcac tcctttccga ccgctttaaa aagaagaata ttatcgtttt agataaactt
     1621 gaattagccg attttcgaac caagagcttt gtagcggtgt taaacaactt aaaaattgtt
     1681 gggaaaacga tttttattat tccggaagaa gatttcaatc tattcatggc tagtcgtaat
     1741 attcctaatg tttatgtgca aaccaaacaa catttgagcg tatacgattt aattaatgcc
     1801 gatacctatg tgacgacgga ag
//
LOCUS       DAOSGN010000191         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_65508_length_1819_cov_3.320862, whole genome shotgun sequence.
ACCESSION   DAOSGN010000191 DAOSGN010000000
VERSION     DAOSGN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65508_length_1819_cov_3.320862"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PK087_03160"
     CDS             complement(<1..1058)
                     /locus_tag="PK087_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH18306.1"
                     /translation="MKVLLVGINSKYIHPAMGIIQLAANTTWPVTLREFTIKDDPQRI
                     AKEISDFHADVVGISVYIWNATLVKTILSQLSQQLTIVVGGPEASYRPAEYFAFSNVD
                     YLVKDEGEQAFHYLLNYLNGLMPLDEVPNLYYRGEEIKFTFNALPDITTIKHDYSLMG
                     NLKHRYLYLESSRGCPYACSYCLASLEKKVRFFPLSQIKKEILWAFHIKTPTVKFLDR
                     TFNCDSQRMIEILSFIQEHDTKQTVFQFEVVADILNEETVRFLKQLRPGLVRFEIGIQ
                     SANETTMEAISRKQNFSQIQTIVQQLKPTITIHLDLIAGLPYEDKPSFINTFNQTLLM
                     FPDELQLGILKALAGTKIV"
     gene            1234..1542
                     /locus_tag="PK087_03165"
     CDS             1234..1542
                     /locus_tag="PK087_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18307.1"
                     /translation="MTKLGEGVLTGIIVSLGSQLLTFINGGFASIIMAAIVAFQGMIL
                     ALNASTFGFGLVFKLLLIGAGSIWLPKLIVSVNMFIDGFKGKSYIANIGWFSASYKPA
                     "
BASE COUNT          589 a          289 c          350 g          591 t
ORIGIN      
        1 acaattttcg ttcccgctaa ggcttttaag attcctagtt gcagttcatc agggaacatt
       61 aacaaagttt gattaaaggt gttgataaac gagggtttat cttcataagg aagcccggca
      121 attaaatcca aatggatggt aatcgtcggt tttagttgtt gcacaatcgt ttggatttgg
      181 gaaaaatttt gctttcgaga gatggcttcc atcgtggttt cgttggctga ttgaatcccg
      241 atttcaaaac gcactaaacc cggccgcaat tgttttaaaa agcggacggt ctcttcattt
      301 aaaatatcgg caacgacttc aaattgaaag acggtttgct tagtatcatg ttcttgaatg
      361 aaggacagaa tttcaatcat tcgctgagaa tcgcaattaa aagtgcgatc taaaaattta
      421 actgtcggag ttttgatatg aaatgcccaa aggatttctt tttttatttg tgaaagcgga
      481 aaaaagcgaa ctttcttttc aagggaagcc aaacagtaag aacaagcata gggacaaccg
      541 cggctggatt ctaaataaag ataacgatgt ttgaggtttc ccatcaacga ataatcatgt
      601 ttgatggtag tgatgtcggg aagagcgtta aaagtaaact tgatttcttc gccgcgataa
      661 tataaattgg gcacttcatc caagggcatc aaaccgttta aatagtttaa taaataatgg
      721 aaagcctgtt caccttcatc tttcacaaga tagtcaacat ttgaaaaagc aaaatattcc
      781 gccggtcgat aactggcttc cggcccccct accacaatcg ttaattgttg agagagttgc
      841 gataagatgg ttttcaccaa tgtggcattc cagatatata cggaaatacc gacaacatcg
      901 gcatgaaaat cgctaatttc cttagcaatc cgctgaggat catctttaat cgtgaattcc
      961 cgtagcgtta ccggccaagt ggtattagcg gctaattgga taatccccat agcggggtga
     1021 atatatttag aattgatacc aactagtaag actttcataa tatccctcgg aattattata
     1081 ccatgttttt ttgatgacgg aaacaagcgc ctcatttttt gcaagaacac tttttaaatt
     1141 tatttcacgg tttgtatttt catttttgat ttatagtatt ataataaagg tgattatatt
     1201 atttggggag ttgtttttaa cgtgagcaaa aaattgacaa aattaggtga aggtgtttta
     1261 acagggataa tagtatcact tggtagtcaa ttactaacat ttataaatgg gggttttgcc
     1321 tctataatta tggctgccat cgtggcattt caaggcatga tattagcatt aaatgcatct
     1381 acttttggat ttggtctggt gttcaaatta cttctaatag gtgctggaag tatttggcta
     1441 ccaaaattga tagtatcagt taatatgttt attgatggat ttaaaggcaa atcctatata
     1501 gcaaatattg gatggttttc tgcaagttat aaacccgctt aataggtgaa ataatgatta
     1561 aaatactact ttacgcgaac cttttaaata tatgtttatc gccatatgtt ggtaaatatc
     1621 tatcaatcaa attcagaaaa aaatggataa taccttacca caagaattag ataaaaaaaa
     1681 gagacaaaaa gtgtatgagt catattgtgc aatttttagt atacttgaag tcataacctt
     1741 tgtcatatca atagcattag gtataaaagt ttatacatat tactacgagt tcgtttatca
     1801 atcagagtta aaaactttg
//
LOCUS       DAOSGN010000192         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_65516_length_1819_cov_2.992630, whole genome shotgun sequence.
ACCESSION   DAOSGN010000192 DAOSGN010000000
VERSION     DAOSGN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65516_length_1819_cov_2.992630"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PK087_03170"
     CDS             <1..395
                     /locus_tag="PK087_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HOH18308.1"
                     /translation="KPVIIQKRAFLACEPRVELSVHWQKKIGVGLFGGEGFIMQKLSG
                     NGLAFIEIDGYEKQYELQENEEMIISTGYLVAMSASCSFNVRSVGTVKNALFGGEGIF
                     NAVVRGPGMVIVQSMPIGNLMHGLSLKK"
     gene            456..1685
                     /gene="pepT"
                     /locus_tag="PK087_03175"
     CDS             456..1685
                     /gene="pepT"
                     /locus_tag="PK087_03175"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOH18309.1"
                     /translation="MNIVNRFLKYVSFPTTSNPNSRTHPSSSEQKLLAAYLVKELQAL
                     GVSDAYMDDYSYVYGTVKSNSSKPIETIGFIAHLDTSCDMSGENIKPRLVHNYDGGDV
                     VLNEALNIVMTTKEFPFLRELKGRTLIVTDGTTLLGADDKAGIAEIMTMVEYFHKHPE
                     VEHGDIKIAFTPDEEIGEGPLFFNIEAWQAKWAYTVDSMKEGLINYENFNAAAARVVI
                     SGINMHPGYAKNHMKNSLLIAMEYNQMLPVAMTPSHTENYEGFYHLNNIEGNVERTEM
                     HYIIRNHDKLKFANQKAQMEKIAAYLNDVYGEKTVSVEIHDQYLNMKELVEKHPDALN
                     VAIEAIKQAGVELTILPIRGGTDGAQLTYRGLVCPNLGTGSYNFHSRYEFVTVEGMEK
                     CTEILINIVKIVANKSN"
BASE COUNT          567 a          334 c          392 g          526 t
ORIGIN      
        1 ataagccagt tattattcaa aaacgtgctt ttttagcttg tgaaccaagg gttgaattat
       61 ctgtgcattg gcaaaagaaa atcggcgtcg gcttgttcgg tggcgaaggt tttattatgc
      121 aaaaactgtc cggcaatggt ttagctttta ttgaaattga tggttatgaa aaacaatacg
      181 agctccaaga aaacgaggaa atgattatca gcaccggtta tttagtagcc atgagtgcct
      241 cttgttcttt taatgtgaga agcgtgggaa cggtaaagaa tgcgctcttt ggtggtgaag
      301 gcatcttcaa cgctgttgta agaggccccg gaatggttat tgtgcaatcc atgcctattg
      361 gtaatttaat gcatggctta tccttgaaaa aataagcctt ttggcttatt ttattactac
      421 aagctttttt attatcacac gatgggagga ttaagatgaa tatcgtcaat cgttttttaa
      481 aatatgtgag tttcccaacc acatccaacc ccaattcacg cactcaccct tcgtcttcag
      541 aacaaaaatt attagctgct tatttagtaa aggaattaca agcacttggt gtgagtgatg
      601 cttatatgga tgattatagt tatgtttacg gtaccgtaaa aagtaatagt tccaagccga
      661 ttgaaaccat cggttttatt gctcatttag acacttcttg tgacatgagc ggagaaaaca
      721 tcaaaccacg actagtccat aattatgatg gcggcgatgt tgttttaaac gaggctttaa
      781 atatcgttat gacaacgaaa gaatttcctt ttcttcggga actcaaaggt cggactttaa
      841 ttgtgaccga cggaacaacc ttgcttgggg ctgatgacaa agcggggatt gcggaaatca
      901 tgaccatggt tgaatatttc cacaagcacc cggaagtaga acacggtgac atcaaaattg
      961 cttttactcc cgacgaagaa atcggggaag gtccgctttt tttcaacatt gaagcttggc
     1021 aagccaagtg ggcttatacc gtcgacagca tgaaagaagg cctcattaat tatgagaatt
     1081 ttaatgcagc agccgcaagg gtcgtaattt ccggtattaa tatgcatccg ggctatgcca
     1141 aaaatcatat gaaaaatagt ttgttaattg ccatggaata taaccaaatg ttaccggtgg
     1201 cgatgactcc cagtcatacc gaaaactatg aaggctttta ccatttgaat aatattgaag
     1261 gtaatgtgga aagaaccgaa atgcactaca tcatccgtaa tcacgataaa cttaagtttg
     1321 caaaccaaaa agcacaaatg gaaaaaatcg ccgcttattt aaatgatgtc tatggcgaaa
     1381 agaccgtgag cgtagaaatt catgatcaat acttaaacat gaaagagctt gttgagaaac
     1441 atcccgatgc tttaaatgtc gccatcgaag ccatcaaaca agcgggcgtg gaacttacca
     1501 tcttaccgat tcgaggcgga acggatgggg cccaattaac ttatcgtggc ttggtttgtc
     1561 ctaatttagg gaccggttct tataatttcc atagccgtta tgaattcgtt acggtggaag
     1621 gaatggaaaa gtgcacggaa atattaatta atattgtgaa aattgttgcc aataaaagta
     1681 attaaaagag ccattgctgg ctcttttaat tataaggatt ctataagctt tacctcatag
     1741 ttttagacaa ctggtcgaga taatgggatt tgaacccatg acctcttggt cccgaaccaa
     1801 gcgcgctacc taactgcgc
//
LOCUS       DAOSGN010000193         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_65637_length_1817_cov_2.254257, whole genome shotgun sequence.
ACCESSION   DAOSGN010000193 DAOSGN010000000
VERSION     DAOSGN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65637_length_1817_cov_2.254257"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PK087_03180"
     CDS             <1..225
                     /locus_tag="PK087_03180"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011412839.1"
                     /note="Enables the production of acetyl-CoA by
                     phosphorylating acetate in the presence of ATP and a
                     divalent cation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOH18310.1"
                     /translation="VLVFTAGIGENSISFRKDIINRLGALGIVLDEEANNTRGKIKEI
                     TKPESKVRAFIVPTDEEVMIARDTVRLANL"
     gene            222..>1817
                     /locus_tag="PK087_03185"
     CDS             222..>1817
                     /locus_tag="PK087_03185"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HOH18311.1"
                     /translation="MKKFRLLIIIFVLLGLVTACKPEKTPVNPESITINSQQSELEVN
                     GSLQLTALVLPAEAEQSVVWSSSDETIATVDNTGKVLGLSAGTVSITATSTKLSSISK
                     SISLEVKTATVHPSSITINSPQKEVDVNSSLQLTALVWPAEAEQSVVWSSSDESIATV
                     DSTGKVLGISPGKVSITATSTKSTSISNKITLKIKPVSIPTDLEDFDVFIENIFLDMI
                     ADDPVNVNFFLRYPENFELGERKVEAIDVSLEAHQTVIAEAKALKESLEYYISVDLTE
                     EQELTYDVTMDYLNKTIAYENYYYYDTPLGSYLGYQAQLPFILAEYHFYSKEDLQDYF
                     DYLLTTQETFENFIAFEVDRANQGFGLNDYLIDGIITQCDTFADAEENYLIAVFNDKV
                     DTLAFLTASEKDEYKANNLNYINNHFVPAYEYLSTELAKLKGKATNNEGLAHFANGKE
                     YYQLLFTEASGTNMTVGQAYNYFRSILDSEYAELMRIYQKNPNLFNIINSPTFLPART
                     YQQTYDHFLESYVNDFPSIGEVVV"
BASE COUNT          589 a          312 c          346 g          570 t
ORIGIN      
        1 gttcttgttt tcacggctgg aattggtgaa aacagcattt ctttccgcaa agatattatc
       61 aatcgcctcg gagctttggg gattgttctt gatgaagaag ccaataatac tcgtggcaag
      121 attaaggaaa ttaccaaacc ggagtcgaaa gttcgagcct tcattgttcc aactgatgaa
      181 gaagtgatga tcgctcggga tacagtgagg ttagcaaatt tatgaaaaaa tttagattat
      241 taataattat ttttgtttta ttgggattgg ttacggcttg taaacctgaa aaaaccccag
      301 ttaatccaga atcgattacg attaacagcc agcaaagcga attagaagtc aatggttctt
      361 tacaattaac agcgcttgtt ttgcctgcgg aagcggaaca aagcgttgtg tggagtagtt
      421 ccgacgaaac gattgcgaca gttgataata ccggtaaagt tttaggcctc agtgcgggaa
      481 cggtatccat taccgccacc agcacgaaat taagttccat cagtaagtct atttcgctgg
      541 aagttaaaac tgccactgtt catccaagct ccattacgat taacagcccc caaaaagaag
      601 tggatgttaa tagttcttta caattaacag cgcttgtttg gcctgcggaa gcggaacaaa
      661 gcgttgtttg gagtagttcc gatgaaagca ttgcgacggt tgatagtacc ggcaaagttt
      721 taggcatcag ccccggaaaa gtctccatta ccgccaccag tactaaatca acttccatta
      781 gtaataaaat tactcttaaa attaaacctg tgtccattcc tacggactta gaagattttg
      841 atgtgttcat tgaaaatatt ttccttgata tgattgctga tgacccagtg aatgtcaatt
      901 ttttccttcg ttatccagaa aattttgaac ttggtgaaag gaaagtggaa gcaatcgatg
      961 tttcattaga agcccatcaa accgttattg ctgaagcgaa agctttgaaa gaaagtttag
     1021 agtactatat aagtgttgat ttaactgaag aacaagaatt aacctacgat gtaacgatgg
     1081 attatttaaa taaaaccatt gcttatgaaa actattatta ttatgatacg ccactaggaa
     1141 gttatttggg ctatcaagca caactaccct ttattttggc ggaatatcat ttttattcca
     1201 aagaagattt gcaagattac tttgactatt tattaactac ccaagaaact tttgaaaatt
     1261 ttatcgcttt tgaagttgat cgtgctaatc aaggatttgg cttaaatgat tatttaattg
     1321 acggcatcat aacacaatgt gatacttttg ccgatgcgga agaaaattat ttaattgctg
     1381 tttttaatga taaagttgat acattagcgt ttttaacagc ctcggaaaaa gatgaatata
     1441 aggcaaacaa tttaaattat atcaataatc attttgtgcc cgcctatgaa tatttaagta
     1501 cagagcttgc taagttaaaa ggtaaagcca ctaataatga aggtttagcg cactttgcca
     1561 acggtaaaga atattatcaa ttattattta cggaagctag cggaaccaat atgacggttg
     1621 gccaagctta taattatttc cgttctatcc ttgattccga atatgccgaa ctgatgagaa
     1681 tttaccaaaa aaatcctaat ttatttaata tcattaattc acctactttt ttaccagcgc
     1741 gaacttatca acaaacctat gatcattttt tagaaagcta tgtcaatgat tttccaagta
     1801 ttggcgaggt agttgtc
//
LOCUS       DAOSGN010000194         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_65872_length_1812_cov_2.737621, whole genome shotgun sequence.
ACCESSION   DAOSGN010000194 DAOSGN010000000
VERSION     DAOSGN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65872_length_1812_cov_2.737621"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PK087_03190"
     CDS             complement(<1..1314)
                     /locus_tag="PK087_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18312.1"
                     /translation="MEIKEKYAELTQKSLTLADFSQIVDLKNQCIMALNQEYQYLCDL
                     LIIDLYIDNRLFDDALALALKTFNLIDRVVFQKIYLSFLDHIIFIYFQKKNFKSAYRY
                     ASLKRNYLNLDNHDEVNRWYLEMAYIGAELNQKDKAQQYLQAILTNYPDEPLKALTIS
                     NLTKLYLDDGLLTEAKKTLSEGIKLVEKLKDNEGKLYCEYLNAKLFVLEKNYKYAKQS
                     FHDLFHKIPVIGDDYLGILNEYLDLLITIEHWDDFDKMVSKYQKSLENCLDLVVKKDF
                     YLNLLRAKILRDKNIRGTARPLLSMLELLDKQISKSQAEAIDVSNEDEKNIEVNQRLQ
                     TMVTKIEKTMVLANMALLSDNERDCFMEFSKHLEPIVGFNEALFIVFSKSSFETLPDF
                     FENFNYVKSFEYKKQRLFERDIPYNSLNDTIVEMVVTANNEIVIDF"
     gene            complement(1422..>1812)
                     /gene="dtd"
                     /locus_tag="PK087_03195"
     CDS             complement(1422..>1812)
                     /gene="dtd"
                     /locus_tag="PK087_03195"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOH18313.1"
                     /translation="GKLISAIESGYLLLVGFKKGDHFDVLPKMVKKIANLRIFEDENG
                     KMNLSLHEVKGSILSISQFTLYANLSKGNRPSFTESMEPNQARIMYHQFNELLKQEGF
                     PILEGLFGAHMDLSFTNSGPVTIIIEF"
BASE COUNT          629 a          315 c          252 g          616 t
ORIGIN      
        1 aaaatcaatg acgatttcat tattggccgt aacaaccatt tctacaatag tatcatttaa
       61 gctgttgtag ggaatatcgc gttcaaataa ccgttgtttt ttgtattcaa atgacttcac
      121 ataattaaaa ttttcaaaaa aatccggtaa agtttcgaaa cttgatttcg aaaaaacgat
      181 aaataaagcc tcattaaaac cgacaatcgg ttctagatgt ttggaaaact ccataaaaca
      241 atcacgttca ttatcactta ataaagccat attcgctaac accatcgttt tttcaatttt
      301 cgttaccatg gtttgcaagc gttgattaac ttcaatgttc ttttcatctt cattggaaac
      361 atcaattgct tccgcttgac ttttagatat ttgtttatcc aacaattcaa gcatcgaaag
      421 caaggggcgt gccgttcccc gaatattttt atcgcgaaga attttagctc ttaacaaatt
      481 taaataaaaa tcttttttga cgactaaatc aaggcaattt tcaagtgatt tttgatactt
      541 agagaccatt ttatcaaagt catcccaatg ttcaatcgta atcaacaaat ctaaatattc
      601 atttaaaatg cctaaataat catcaccgat aacgggaatt ttgtgaaaca aatcatgaaa
      661 actttgttta gcgtacttat aattcttttc caaaacaaat aatttcgcat ttaaatattc
      721 acaatataat ttaccttcgt tatcttttaa tttttcaacc aatttaatac cctcggaaag
      781 tgtttttttg gcttccgtca ataaaccatc atccaaatat aatttggtta aattgctgat
      841 ggtgagggct tttaaaggtt catcgggata attcgttaaa atggcttgta aatattgttg
      901 agctttatct ttttggttta gttctgctcc aatataggcc atttccaaat accaacgatt
      961 aacttcatca tgattgtcaa gattaagata attccgtttt aaagaagcat atcgataagc
     1021 acttttaaag ttcttctttt gaaaataaat aaagatgata tgatctagaa aagaaagata
     1081 aattttttgg aaaacaacac ggtcaattag attaaaagtt ttaagcgcca acgctaaggc
     1141 atcatcaaaa aggcgattat caatatacaa atcaataatc aaaagatcac ataaatattg
     1201 atattcttga tttaaagcca taatacattg atttttcaga tcaacgattt gggaaaagtc
     1261 agccaaggtc aaggactttt gcgtcaattc agcatatttt tctttgattt ccataaaaaa
     1321 accctttcca aacttatcta attgattata gcataattaa gcgtaaaagt taagattaaa
     1381 aaagtggcca aagccgcttt tagctacttg gctttagaca cttaaaattc aatgatgata
     1441 gttaccggac cggaattggt aaagcttaaa tccatatgtg ctccaaaaag tccttccaaa
     1501 ataggaaacc cttcttgttt aagtagctca ttaaattggt gatacattat acgtgcttga
     1561 tttggctcca tactttcagt aaaagacggt cgatttcctt ttgataaatt agcataaagc
     1621 gtaaattgag aaattgataa aattgatccc ttaacttcat gcaaactaag gttcattttt
     1681 ccgttctcat cttcaaaaat acgcaaatta gcaatttttt taaccatttt ggggagcaca
     1741 tcaaaatggt cccctttttt aaaacccact aacaataaat agccggactc aattgcactg
     1801 attagttttc cc
//
LOCUS       DAOSGN010000195         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_66579_length_1799_cov_2.454128, whole genome shotgun sequence.
ACCESSION   DAOSGN010000195 DAOSGN010000000
VERSION     DAOSGN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66579_length_1799_cov_2.454128"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PK087_03200"
     CDS             complement(<1..442)
                     /locus_tag="PK087_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOH18314.1"
                     /translation="MQAVANKQKLILESPNLYRSILILSLPIFLSNFMKAFNDFIDMY
                     FVSNFVQENVSEAIAAISVTGPVFSISQALAGGMMIAGAAMMSQFLGAKQRERANKVA
                     GSLLRLCLLASLLVNILLYLLAPMIMRWMGASGIQHQLMVTYVRV"
     gene            complement(473..1246)
                     /locus_tag="PK087_03205"
     CDS             complement(473..1246)
                     /locus_tag="PK087_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18315.1"
                     /translation="MIKEAIGILDMGMESFPLLFPLSKTFKYERFVYANDLLNQPYEG
                     LEPEAIVGFVKENVERLQKQNLKLLIVGSDVIWEYAQDFLKTLSIPVFNIVDTLIEYI
                     NNHYEQKNMVLLAKNSILEANLYQKNFKYNHLYSLPSDELEAIVLNKMTKTTQSFYVA
                     KTSLLPALKKEVDIIITSTPLLRLVKTEIDEYLRGAEITDVLAIWEDKIKSSGLVLYE
                     KGRGRIEVLGNVPKKQWIQQTVGEPFKYKYLETKANQSN"
     gene            1470..>1799
                     /locus_tag="PK087_03210"
     CDS             1470..>1799
                     /locus_tag="PK087_03210"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOH18316.1"
                     /translation="MRKILLSEITKTVMELLLEANTHLGEDVFSRLKQAITQEESKLG
                     KSIIEQIVKNDEIAKDELVPMCQDTGMVVIFLEIGSQVVWEGDLDQAINEGVRLAYQK
                     GYFRKSVV"
BASE COUNT          562 a          355 c          326 g          556 t
ORIGIN      
        1 ggacccgaac ataggtgacc atcaattggt gttgaatgcc actagctccc atccagcgca
       61 taatcattgg cgctaaaaga tataaaagaa tatttaccaa aaggctcgcc aaaagacaaa
      121 ggcgtaagag cgagccggcc actttattgg cacgctctct ttgtttagcc cccaaaaact
      181 gggacatcat cgcggccccc gcaatcatca tccccccggc taaagcttgc gagatgctga
      241 aaaccggccc agttaccgaa atcgccgcaa tcgcttcact gacattttcc tgtacaaaat
      301 tgctgacaaa atacatatca atgaaatcat taaaagcttt cataaaattg cttaaaaaga
      361 tgggaagcga taatattaaa atacttcgat atagattggg gctttctaag attaattttt
      421 gtttattggc aactgcttgc ataaatatcg tccttttaat gaatgtaata agttaatttg
      481 attgattggc tttcgtttct aggtatttgt atttaaaagg ctcaccaaca gtctgttgaa
      541 tccattgttt tttcggaaca ttccccagca cttcaatccg tccccgacct ttttcataga
      601 gaacaaggcc actgcttttg attttgtctt cccaaatggc aagaacatcc gttatttccg
      661 ctccccgcaa atattcgtca atttccgttt tcacaagcct caacaagggg gtactggtaa
      721 taataatatc gacttctttt ttaagggcgg gaaggaggct agttttcgca acataaaaac
      781 tttgcgttgt tttggtcatc ttattaagaa caatggcttc caattcatcg cttggcaaac
      841 tatataaatg attatatttg aaattcttct ggtagagatt agcttccaaa atcgagtttt
      901 tcgccagcaa aaccatgttt ttttgttcat aatgattatt gatgtattcg ataagagtat
      961 cgacgatatt aaaaacagga atcgagagcg tcttcaaaaa atcttgtgcg tattcccaaa
     1021 taacatcact accgacaatt aataatttga gattctgttt ttgcaagcgt tccacatttt
     1081 ctttcacaaa accaacaatg gcctccggtt ctaaaccttc atagggttga tttaataaat
     1141 cgttagcata aacaaagcgt tcatatttga atgttttact taaaggaaat agtaagggaa
     1201 agctttccat ccccatatct aatatcccaa tcgcctcttt aatcattgag ttcacctctt
     1261 tttgttatta tagcctaaat gggcattttc ttcaatattt tttattaaaa ccgctcaaat
     1321 ccttgtcata tgcacccgta atcgcttttc acagtccaaa ctgataaata cttggctttt
     1381 gtaaaaagcg aagacaaacg attttttttg aaattttctc ctctttagtg tataatgaag
     1441 ccaagcaaaa tttgtgatga ggtggcaaaa tgcgcaaaat cctattaagt gaaataacga
     1501 aaacggtgat ggaattatta ctggaagcga acacccattt gggggaagat gttttttcaa
     1561 gattaaaaca agccatcacc caagaagaaa gcaaactcgg taaatccatt attgaacaaa
     1621 ttgtgaagaa cgatgaaatt gcgaaagatg aactggtgcc gatgtgccaa gacaccggga
     1681 tggtggtaat ctttttagaa atcggttccc aagttgtttg ggagggcgat ttagaccaag
     1741 ccattaatga aggtgttcgt ttagcttatc agaaaggcta ttttcgaaaa tcggttgtt
//
LOCUS       DAOSGN010000196         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_66977_length_1792_cov_2.265400, whole genome shotgun sequence.
ACCESSION   DAOSGN010000196 DAOSGN010000000
VERSION     DAOSGN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66977_length_1792_cov_2.265400"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="PK087_03215"
     CDS             <1..137
                     /locus_tag="PK087_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005981739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HOH18317.1"
                     /translation="TTNIAVACKATQCLAKPGMGTDEGFMILKNEMNRIVALANSRKR
                     "
     gene            142..399
                     /gene="citD"
                     /locus_tag="PK087_03220"
     CDS             142..399
                     /gene="citD"
                     /locus_tag="PK087_03220"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HOH18318.1"
                     /translation="MKIGVAGTLESSDVMITVREADALKITIESIVFAYFSEQIHNTI
                     LSTLREMNITNVHVLCQDKGALDYVIKARLKTAIERMKQHA"
     gene            392..1249
                     /locus_tag="PK087_03225"
     CDS             392..1249
                     /locus_tag="PK087_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOH18319.1"
                     /translation="MRRSLLFIPGNNPAMLQNADVFESDGLILDLEDAVHINEKDSAR
                     MLVSHYLESKQTHSLEMIVRINGLDTPYFQKDLQAVVCQQLDTIMLPKAKVKDVLDLS
                     KMLDEIEQTKQISKEIKIIPIIELAQSVLEVNELARFPRVNGILLGAEDLSTDLEIER
                     TKPGLEIIYPRFCVIYACKAQKIDAIDTPFTDTKDEQGLSKDCETAKMFGMNAKTAIH
                     PNQVSIINDVFSPSEKNIAWAKRVIEAKKKAEASGLAVFSLDGKMIDKPIIDRAINIL
                     KKAEKFQLL"
     gene            1257..>1792
                     /locus_tag="PK087_03230"
     CDS             1257..>1792
                     /locus_tag="PK087_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOH18320.1"
                     /translation="MKNSLQREVPVGFKPFVSPTAFQKKPRIMIPEKNTNHQIVFFES
                     LEAMFKEMGISDGMTLSFHHHLRNGDEVLNQTAKVVEKMGLKNLHFAPSSIFPNNACL
                     SSLIKNKNITSITTNYLNGDVAKTISQGYLENLLIMETHGGRARSIEAGELAIDFAIL
                     ATPAVDCMGNGTGSLGKNA"
BASE COUNT          599 a          303 c          357 g          533 t
ORIGIN      
        1 cgaccaccaa tattgccgtt gcttgcaaag ctacgcaatg tttagctaag ccgggaatgg
       61 gaactgatga aggttttatg attttaaaaa atgaaatgaa tcgaattgtt gccttggcaa
      121 acagtagaaa gcgataaaaa aatgaaaatt ggtgtggctg ggacattaga atcaagtgat
      181 gttatgataa cagttcggga agcggatgct ttaaaaatca cgattgaaag catcgttttt
      241 gcttatttct ccgaacaaat tcataatacc attttatcaa ccttacgaga aatgaacata
      301 actaatgttc atgtgctatg ccaagataaa ggagcgcttg attatgttat caaagctcgt
      361 ttaaaaacgg ccatcgaaag gatgaaacaa catgcgtaga agtttacttt ttattccggg
      421 aaataatccg gccatgcttc aaaatgccga tgtttttgaa agtgatggcc ttattttaga
      481 cttggaagac gccgttcata ttaatgaaaa ggatagtgcc cgtatgctgg tttctcatta
      541 tcttgagtca aaacaaactc atagtttaga aatgattgtc cggattaacg gtttagatac
      601 tccttatttt cagaaagatt tacaagccgt ggtttgccag caactagata ccattatgct
      661 tcccaaggct aaagttaaag atgttttaga tctttcaaag atgcttgatg aaatcgaaca
      721 aactaaacaa ataagcaaag aaataaaaat tattccgatt attgaactag ctcaatcggt
      781 tttagaagtt aatgaattag ccagatttcc tcgggtcaat gggattttat taggagccga
      841 agatttatca acggatttgg aaattgaaag aacaaaacct ggtttggaaa taatatatcc
      901 acgcttctgc gttatttatg cttgcaaagc gcaaaaaatt gatgccattg atactccttt
      961 tacagataca aaagatgaac aaggcttgag taaggattgt gagacggcaa aaatgtttgg
     1021 gatgaatgcg aagacggcca ttcaccccaa tcaagttagc atcattaatg atgtcttttc
     1081 gccaagtgaa aaaaatatcg catgggctaa acgagttatt gaagccaaga aaaaagctga
     1141 agccagtggt ttagctgtct ttagtcttga tggtaaaatg attgataaac caattattga
     1201 tcgagcgatt aatatcttaa aaaaagctga aaaattccaa ttgctttagg tgaaatatga
     1261 aaaacagtct acaacgagaa gtcccggttg gctttaaacc ctttgtttcc ccaacggctt
     1321 ttcaaaaaaa accacgaata atgattcctg aaaaaaacac caatcatcag attgtttttt
     1381 ttgaatcttt ggaagcgatg tttaaggaga tggggatttc ggatggcatg actctatcat
     1441 ttcatcatca ccttcgtaat ggtgatgaag tcttaaatca aactgcgaaa gttgtagaaa
     1501 aaatgggttt aaaaaatcta cactttgctc ctagttccat tttccccaat aatgcttgtt
     1561 tatcatcatt aattaaaaat aaaaacatta cgtccattac cactaattat ctcaatggcg
     1621 atgttgctaa gacgattagc caaggttatt tagaaaatct tctgattatg gaaacccatg
     1681 ggggtcgtgc tcgctccatt gaagccggtg agttagcgat tgattttgcc attttagcaa
     1741 ccccggctgt tgattgtatg ggcaatggca ccggttccct cggtaagaat gc
//
LOCUS       DAOSGN010000197         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_67707_length_1779_cov_3.087587, whole genome shotgun sequence.
ACCESSION   DAOSGN010000197 DAOSGN010000000
VERSION     DAOSGN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67707_length_1779_cov_3.087587"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    complement(102..326)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(374..>1779)
                     /locus_tag="PK087_03235"
     CDS             complement(374..>1779)
                     /locus_tag="PK087_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOH18321.1"
                     /translation="GTNYENMNLYKRDFPEYQLFHLTQNYRSTQHILDGCNLLISNNY
                     NREPKKLFSTDPGSPDDVHIYQAFTEKEEVNYILDRIFSLKLKGVDYQKMAILYRNSS
                     LLRNLELGIIQMGLPYQVYGGVSYLRRREVKDIIAYFKLLLNPDDVYSFQRVINVPSR
                     LLGEVTVAKVLEIKKKYHLSLWQAIDACQALISDKRFTALLEFKNLITAFSLRLEEEH
                     LWTVFDELLAFIKYEDYLKEDEDFEERWENVQEFKSILLQIEESQGELTRKERLQAAF
                     DEAILSDDKLQNQRQNPQGLILSTIHSVKGLEFDYVFVIGLEENIFPNVFRFESEAEL
                     EEERRIAYVAFTRAKKRLYLLSAKTRLLYGKHFTNKTSRFVLEFAGMNRLNVDIQAEV
                     VADYAQEEARGIVSVRPQDEVKTTDGDYRVGDKVIHEQFGEGIIIAINRNIGQIFFDK
                     TKKLSKILLTHSGLKKK"
BASE COUNT          546 a          381 c          291 g          561 t
ORIGIN      
        1 tgacagtgtc gccacttttt cgatacccgg aaattcctga taaaatttaa tcatcttttc
       61 ctccttgcta taaaaaagca aaaagtccct taacaattat gttaagggac gaaaattccg
      121 cggtaccacc ctgcttatgg ttaaaaaacc atcactcttg atcaatcaat caattcatcg
      181 ttaacgggat ttcccggttt aaactaccaa aaattcgcct aaacagctcc aaagtgtaca
      241 ttctatattg gcttgtcctc attttcacca accatgagat ctctaaaagc aaggtcaata
      301 ttttttctct ttttcttcgc ttttttatat atgacccatt gtaaacctat ttcacaaaaa
      361 aagcaagacc tttttatttt ttctttaaac ccgagtgcgt taataaaatt ttagacagtt
      421 tttttgtctt atcaaaaaag atttgaccaa tgttgcgatt aatagcaatg atgatgccct
      481 caccaaactg ttcatgaata actttatccc caactcggta atcaccatcc gtggttttca
      541 cttcatcctg cgggcgaaca gaaacaatcc cgcgcgcttc ttcttgcgca taatccgcaa
      601 caacttctgc ttgaatatca acattaagcc ggttcatgcc ggcaaactcc aaaacaaaac
      661 gcgaagtttt attggtaaaa tgcttaccat ataataatct ggttttcgca cttaaaagat
      721 ataatcgttt tttggctctt gtgaaagcga cataagcaat gcggcgttct tcttccaatt
      781 ccgcttcgct ttcaaaacga aagacattcg ggaagatgtt ttcttccaag ccaataacaa
      841 agacatagtc aaactccaaa cccttgacgg agtggatggt ggataaaatt aggccttggg
      901 ggttttggcg ttggttttgg agtttatcat cggataaaat ggcctcatcg aaagccgctt
      961 gtaaacgttc ctttcttgtc aattctcctt gtgattcttc aatttgtaag agaatgctct
     1021 taaactcctg cacattttcc cagcgctcct caaaatcctc atcttctttt aaatagtctt
     1081 catatttaat aaaggctaac aattcatcaa aaaccgtcca taaatgttct tcttccaggc
     1141 gcaaggaaaa agccgtaatg aggttcttaa attccaatag agccgtaaat cgtttatcgg
     1201 agattaaggc ctgacaagcg tcaatcgctt gccataaaga taagtgatat ttctttttaa
     1261 tctccaaaac tttcgcaacc gtcacttcac caagcaaacg agacggtaca ttaatcactc
     1321 tttgaaaact ataaacatca tcgggattaa gcaatagttt aaaataagca attatatctt
     1381 tgacttcccg acggcgtaaa taggaaacac cgccatacac ttggtagggt agtcccattt
     1441 gaataatgcc cagttccaaa ttgcgtagta aactgctgtt gcgatataaa atggccatct
     1501 tttggtaatc aacacctttt agtttcaacg aaaaaatgcg atccaatata taattgactt
     1561 cttctttttc ggtaaaggct tgataaatat ggacatcatc cggtgaaccc gggtcagtag
     1621 aaaacagttt cttcggttca cgattgtaat tgttactgat taacaaatta cagccatcta
     1681 aaatatgttg ggtggaacga taattttgtg tgaggtgaaa caattgatat tccggaaaat
     1741 cccgtttata caaattcatg ttttcgtagt ttgtgcccc
//
LOCUS       DAOSGN010000198         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_67759_length_1778_cov_3.026117, whole genome shotgun sequence.
ACCESSION   DAOSGN010000198 DAOSGN010000000
VERSION     DAOSGN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67759_length_1778_cov_3.026117"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PK087_03240"
     CDS             complement(<1..278)
                     /locus_tag="PK087_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18322.1"
                     /translation="MFLANFNDGAYIFFSIIALAVTATLGYFLIRSVFRERAKFREEK
                     TVLLEGLLSKSELTSAISGYIAKGSLETTFALIYVDIDKFTDIINAFG"
     gene            complement(341..>1778)
                     /locus_tag="PK087_03245"
     CDS             complement(341..>1778)
                     /locus_tag="PK087_03245"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HOH18323.1"
                     /translation="IFIRFIPLRSGFGYYLLGEDITAREQDYLMNRNMALYTKTTKLP
                     NKNYLIAKLKDLFNNEESLGLLNSLVAFDISSFKNINNLFGMKIGDETLITLANIVKE
                     SLKDKNATLFNTEADNFVVLFERVESFNEVKKWVESTLELLAHPVEINKNLLILDVKM
                     GIFNIESEIYGNLTPTMAYDHAMVALKRAKNSRTAKYVTYDLGLGQYITREQIMETDL
                     VKAIQEKEFVMYLQPQFDNEQERIVGFESLIRWNNPKYLLESPAHFIALAEQNNLIIE
                     IGKFVILDTFRIAKEFEPYNVHISINISPVQLLQAGFVSEIVEIYDRFELKPHAISVE
                     ITETFLMTNFDSVIEKLKLLKNHGFNIHLDDFGTGYSSMLYLKDLPIDTIKIDKEFVR
                     HLNTDKFSRAIVSKVISMAKSLDLDVIAEGVEDDKQNQFLVKSGCNVIQGHLISAAVP
                     ESVALKLINEYNINKTKEIKVKSKTKEK"
BASE COUNT          572 a          354 c          299 g          553 t
ORIGIN      
        1 tccccaaagg cattgataat atcggtaaat ttatcaatat cgacataaat gagggcgaag
       61 gtcgtttcca acgagccctt ggcaatatag ccggaaatgg cactcgtcaa ttcggacttc
      121 gacaataagc cttctaacaa aaccgtcttc tcttcacgga acttagctcg ttcgcgaaaa
      181 accgagcgaa ttaaaaaata tccgagcgtc gccgtaactg cgagggcaat gatcgaaaaa
      241 aagatatagg caccatcatt aaagttggca agaaacatgg gatcacctct tgtatattgt
      301 atcacaatca tcaaaaaaag atattgtttt ttaaaacaaa ttatttttct tttgtttttg
      361 atttcacttt tatttcttta gttttattaa tattatactc attaatgagt tttaaagcga
      421 cactttcggg aacagcggca ctgattaaat gcccttgaat gacattacag ccgcttttaa
      481 ccaaaaattg gttttgttta tcatcttcaa caccttccgc aatcacatcc aagtccaagc
      541 tcttggccat ggaaataact ttggaaacaa tagcccgcga gaatttatcg gtattgagat
      601 gacgaacaaa ttccttatca atcttaatgg tatcaatcgg taaatctttt aagtagagca
      661 tcgaactgta accggtcccg aagtcatcca aatgaatatt aaaaccatga tttttcaaaa
      721 gctttaattt ttcaatcacc gagtcaaaat tagtcatcaa gaaagtttcg gtgatttcca
      781 ccgaaatggc atgcggtttt aattcaaagc ggtcgtatat ttccacaatc tcgctgacaa
      841 acccggcttg caataattga actggagaaa tattgatgga aatgtgcaca ttataaggtt
      901 caaattcttt cgcaatccgg aaagtatcta aaataacgaa tttaccgatt tcgataatta
      961 agttgttttg ttcagctaag gcaataaaat gagccggtga ctccaataaa tacttcggat
     1021 tattccagcg aattaaggat tcaaaaccaa caatccgctc ttgttcatta tcaaactgcg
     1081 gttgcaaata catgacaaat tctttttctt gaatggcctt gaccaaatcg gtttccataa
     1141 tctgttcgcg ggtaatatac tgtcccaaac cgaggtcata agtaacatat ttagccgtcc
     1201 gcgagttttt ggcccgtttt aaagcaacca tcgcatgatc ataagccatg gttggtgtta
     1261 aattgccata gatttcactt tcaatattaa agattcccat tttcacatcc aaaattaata
     1321 agttcttatt aatttcgacc ggatgcgcta acaattcgag agtgctttcc acccattttt
     1381 taacttcatt aaaactttca acgcgttcaa acaaaaccac gaagttatcc gcttccgtat
     1441 taaagagcgt cgcattcttg tcttttaagg attccttaac aatattagca agggtaatta
     1501 aggtttcatc accgattttc atcccgaata aattattgat gtttttaaag gaactgatat
     1561 caaaagcaac caaggaatta agcaatccca atgattcctc attgttaaac aagtccttta
     1621 gtttggctat taaatagttt ttattcggta atttcgtcgt ttttgtgtag agagccatat
     1681 tccggttcat caaataatct tgttcgcggg ccgtaatatc ttcccctaac aaataataac
     1741 cgaagccgga gcgtaaggga ataaagcgaa tgaagatt
//
LOCUS       DAOSGN010000199         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_67804_length_1777_cov_3.882695, whole genome shotgun sequence.
ACCESSION   DAOSGN010000199 DAOSGN010000000
VERSION     DAOSGN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67804_length_1777_cov_3.882695"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PK087_03250"
     CDS             <1..1203
                     /locus_tag="PK087_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOH18324.1"
                     /translation="NQYPHLPLFAFQLIMRCPQYDSSDEEPEYYQHSGRHIYLNGYYQ
                     NKKQLGILSLDEAQHWETIKVEERYLNDFLSRRALNKQINIFTLDLLAQGAIDFLVIP
                     QDDASEYGYTAMDQVDIRTVIRQKQLMHQAYMYPGADEAGCVLVSRMINHLEQKTPAF
                     FVKYPSPTTPMVIPCLEDRYVDVTIKYHIACAGGVTVPSLADADLVLLPFLGATNMIP
                     RPSNEPSLDVDVMCNFTEMMEFANYAINKAYPVAIADLVYLNGGSIDILNFLESKNLT
                     MKVASYAGWNTSSNSLGTAIAQAIQFWYRGHSPQHEAFLIKRYVEDVGYCGMVRALVT
                     KQLGTYGMNYFDVKEKEGQAAQLVNQELQAFITQYLPSIKDRFYLSKVNLPWMRMFEV
                     DFKINLKD"
     gene            1206..>1777
                     /locus_tag="PK087_03255"
     CDS             1206..>1777
                     /locus_tag="PK087_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18325.1"
                     /translation="MKKLAIILSLFIIVVALSWGFNVHAHSMSVNKTEIINSGEAASG
                     VSFESYRGTVINDAGTQSHQRISWVKKSVDAKAKIVAWSILEKTKIKGANMLNLARDF
                     EEKNPGWRVLAGINGDYYDPPSKIPVNAFVQQGDVVRATNFTQPRYFSVGFTDDSSGF
                     VSSKTNRLENKFSLSIYDDKKERIILEVPI"
BASE COUNT          512 a          323 c          393 g          549 t
ORIGIN      
        1 aaccaatatc ctcacttacc gctgtttgcc tttcagctca ttatgcgctg tccacaatac
       61 gattcaagtg atgaggaacc cgaatattat caacattcgg gtcgccatat ttatttaaat
      121 ggttattatc aaaacaaaaa acagttaggg attctatcct tggacgaagc tcaacattgg
      181 gaaacgatta aggttgagga gcgctattta aacgactttt taagtcgacg ggcgcttaac
      241 aaacaaatta atatttttac tttggattta ttagctcaag gggcgattga ttttttggtt
      301 attccccaag atgatgccag cgaatacggt tatactgcca tggatcaagt tgatattcgg
      361 accgttattc gccaaaaaca actcatgcat caagcttata tgtatccggg agcggatgaa
      421 gccggctgtg ttttagtgag tcggatgatt aatcatctag aacaaaaaac gcccgccttt
      481 tttgtgaagt atccttcccc gacgacccct atggttattc cttgtttaga agaccgttat
      541 gttgatgtga ccatcaaata ccatattgct tgtgcgggtg gtgtcactgt gccttcctta
      601 gccgatgccg atttagtttt gttgccgttt ttgggggcga ctaacatgat tcctcgccct
      661 agcaatgaac cgagtttgga tgtggatgtt atgtgcaact ttacggaaat gatggaattt
      721 gccaattatg caattaataa agcttatccg gttgcgattg ctgatttggt ttatttaaac
      781 ggtggttcca tcgatatttt aaactttttg gaaagcaaaa acctcacgat gaaagtagcg
      841 tcttatgccg gttggaatac ttccagtaac tcgttaggga cagcgattgc gcaagcaatt
      901 caattttggt atcgcggtca cagcccgcaa catgaagctt tcttaattaa acgctatgtg
      961 gaagatgtcg ggtattgtgg aatggtgcga gctttagtta ccaagcaact gggaacttat
     1021 ggaatgaatt actttgatgt caaggaaaaa gaaggccaag cggctcaact tgtcaatcaa
     1081 gaactacaag ctttcatcac tcaatatctt cccagcatta aagatcgatt ttatttaagc
     1141 aaggttaatt taccttggat gcggatgttt gaagttgatt ttaaaattaa tttaaaggat
     1201 taattatgaa gaagttggcg attatcttaa gtcttttcat cattgttgtt gccttgagct
     1261 ggggatttaa tgtccatgct cactcgatga gtgttaacaa gacagaaatt ataaacagtg
     1321 gcgaagccgc ttcgggggtt agctttgaaa gctatcgcgg tactgtcatc aatgatgccg
     1381 gaacccaaag ccatcagcgg attagttggg ttaagaaaag tgttgatgca aaagctaaaa
     1441 tcgttgcttg gagtattctt gagaaaacaa aaatcaaggg agcgaatatg ttaaatttgg
     1501 cccgtgattt tgaagagaaa aatccgggat ggcgtgtttt agccggcatt aatggtgatt
     1561 attatgatcc accaagcaaa atccccgtca atgctttcgt tcagcaaggg gatgttgttc
     1621 gagctactaa ctttactcag ccccgctatt tttccgtagg ctttacggat gacagtagcg
     1681 gctttgttag ttcgaaaacg aaccgtttag aaaataaatt ttctttaagc atttatgatg
     1741 ataaaaaaga acggattatt ttggaagtcc caattca
//
LOCUS       DAOSGN010000200         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_67888_length_1776_cov_2.815224, whole genome shotgun sequence.
ACCESSION   DAOSGN010000200 DAOSGN010000000
VERSION     DAOSGN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67888_length_1776_cov_2.815224"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PK087_03260"
     CDS             <1..1110
                     /locus_tag="PK087_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOH18326.1"
                     /translation="TQYIPKWEKPENIRDFQFVHAVTKTKELVEEIKEKHQPDLIILG
                     YHGGLERDLDTDALLVEDTGENLGHRIIESVPDLDIVLTGHQHRIICKKKNKTLVTQP
                     GGSAQYLGKVEVTMEFSGKWQVKEYQAKMLNTTNVAPDPLVNQIIADVEKATQLFLDE
                     PIGYVPHNDLEVKDGFLARLYKHKIVSFINRVQLEVSKAQISLTALANHVSGFKQRIT
                     IRDILNTYVYPNTLVVKRIDGKTLKAALEKNAQYFVIVNDAITVNPKFIYPKAEHYNY
                     DMFDGIEYTIKVSNPEGAKILNLCYQNRVIKDDDCFHIVMNNYRSTGGGEFLMYKNLP
                     VVKEINQDVAEIMIDYIRKTKDIIQDDPKNIRIIK"
     gene            1330..>1776
                     /locus_tag="PK087_03265"
     CDS             1330..>1776
                     /locus_tag="PK087_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18327.1"
                     /translation="MTKSNRFSKLLLIFLVLVLSFGLVGCLESADAKAAKAVDETILA
                     LPETVAMTDEDAIEAARSAYDALTEAQKALVKNLTTLTTKEADLRALKDAASALAVDN
                     LITALPAVVKLADENSIVAARSAYNALTEAQKALVKNLTALTTKENT"
BASE COUNT          580 a          311 c          384 g          501 t
ORIGIN      
        1 actcagtata ttcccaaatg ggaaaaaccg gaaaacattc gtgatttcca atttgttcat
       61 gccgttacca agacgaaaga acttgtggaa gaaatcaaag aaaaacatca accggattta
      121 atcattctcg gttatcatgg tggtttggaa cgagatttag ataccgatgc tttattggtg
      181 gaagatacag gggaaaattt aggccatcgc attattgaaa gtgtgcctga tttggatatt
      241 gttttaaccg gccatcagca tcggattatt tgcaagaaga aaaataaaac cttggttacg
      301 caaccgggcg gatccgctca atatttaggg aaagtggaag tgaccatgga gttttcgggg
      361 aagtggcaag ttaaggaata tcaggctaag atgttaaata caaccaatgt tgcgcccgat
      421 cccttagtta atcaaatcat tgctgatgtg gaaaaagcga cccaactatt tttggatgaa
      481 ccgattggtt atgttcccca taatgattta gaggtcaagg atggcttttt agcccgtttg
      541 tacaaacata aaattgtttc ctttatcaac cgcgtgcaac tggaggtaag caaagcgcaa
      601 atcagtttaa cggcgctcgc caatcatgtc agtggcttta aacaaaggat taccatccgc
      661 gatattttaa acacttatgt ttatccgaat acactggttg tgaagaggat tgacggcaaa
      721 acactcaaag ccgctttaga aaaaaatgct caatactttg tcattgttaa cgatgccatc
      781 accgttaatc ctaaatttat ttacccgaaa gcggaacact ataactatga tatgtttgat
      841 ggaattgaat atactatcaa agtatccaat cccgaaggag ccaaaatttt aaatctttgt
      901 tatcagaatc gagtgattaa agatgatgat tgttttcaca ttgtgatgaa caattatcgc
      961 tccaccggcg gaggcgagtt tttgatgtat aaaaatttac cggttgtgaa ggaaatcaat
     1021 caagatgttg cggaaatcat gattgattat attcggaaga ctaaagacat cattcaagat
     1081 gatcctaaaa atattcgcat cataaaataa ttagtggcca agcggccact gatattgttt
     1141 ttactggggc aggggtttta aagggtgctc taaaggaaaa aaactaaaaa ttaatcggaa
     1201 aaaattggtt aataaaattg gcaagttttt gataatttct ctttaaggat ttttagctat
     1261 gaggtaggtt gcgaaaaatg cgggtgcatg ctataattaa ttgaacttat ttatgtgaaa
     1321 ggagattata tgaccaaatc aaacaggttt tcaaaattac tactcatttt tctggtgttg
     1381 gtgctaagct ttggcttagt aggctgcctt gaatcggctg atgccaaagc cgctaaagcg
     1441 gtcgatgaaa cgattttagc attaccggaa acggtagcaa tgactgatga agatgccatt
     1501 gaagcggcaa gaagcgctta tgatgcttta actgaagcgc aaaaagctct agtcaaaaac
     1561 ttaacgactc tcaccaccaa ggaggcggac ttaagggcct tgaaagatgc ggccagtgcc
     1621 ttggcggttg ataatttaat taccgctctc ccggcggtgg tgaagcttgc tgatgaaaat
     1681 agtattgtcg ccgcaagaag cgcttataat gccttaaccg aagcccaaaa agctttagtt
     1741 aaaaatttaa cagcccttac caccaaggaa aatacc
//
LOCUS       DAOSGN010000201         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_68485_length_1766_cov_2.839860, whole genome shotgun sequence.
ACCESSION   DAOSGN010000201 DAOSGN010000000
VERSION     DAOSGN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68485_length_1766_cov_2.839860"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PK087_03270"
     CDS             <1..860
                     /locus_tag="PK087_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18328.1"
                     /translation="IVGIDNLEVTKLLMKQVEEWAIERGLNQIIGPIGFSDLDKQGLL
                     VEGFEEESSTITLYNHPYYMKHLETLGFRKDIDWVEYQVFTPTSMNDRITRLSAVITN
                     RYGYKVVKVKKRREIKKYARDALLLINESFGHLYGVVPLNDRQIDDTIKQFITLVNLE
                     FLYFVTTKTDELIGFGLMVPSLNQAMKKSNGKLFPFGFLRVLKALKTYRVLDMYLVGV
                     KPQYQKLGVNALILFEGIKSAIKCGVKFAETGPELETNLEVRSLWREFETRQHRRRRC
                     YLRDLTSSS"
     gene            complement(975..1142)
                     /locus_tag="PK087_03275"
                     /pseudo
     CDS             complement(975..1142)
                     /locus_tag="PK087_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     misc_feature    1436..1506
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1522..>1766
                     /gene="rplU"
                     /locus_tag="PK087_03280"
     CDS             1522..>1766
                     /gene="rplU"
                     /locus_tag="PK087_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOH18329.1"
                     /translation="MYAIIETGGKQYRVTTNDVVYVEKLNAAENEEIVFDKVLMVDGK
                     FGSPYLEGAKVTARVEKNGKNKKIIVFKYKAKKNYHK"
BASE COUNT          539 a          267 c          369 g          591 t
ORIGIN      
        1 caattgttgg tattgataat ttagaagtta ctaaattgtt gatgaagcaa gtagaagaat
       61 gggcgattga acgcggtttg aatcaaatta tcgggccgat tggctttagt gatttagata
      121 aacaaggttt attggtagaa ggctttgaag aagaaagcag caccattaca ttatataatc
      181 acccttatta tatgaaacat ttggaaacac tcggctttag aaaagatatt gattgggtgg
      241 agtatcaggt ttttacccca acatcaatga atgatcgcat tacccggctg tcagctgtga
      301 ttactaaccg ctacggttat aaagttgtga aagtaaaaaa acgtcgagaa attaaaaagt
      361 atgctcgtga tgctctcctt ttaattaatg aatcgtttgg tcatttatac ggcgttgtgc
      421 ctttaaatga ccgccaaatt gatgatacga tcaaacagtt cattacgctt gttaatttgg
      481 aatttttata cttcgttacg acgaaaaccg atgagctaat cggttttggg ctcatggttc
      541 catctttaaa ccaagcgatg aaaaagtcaa acgggaaatt gtttcctttt ggttttttgc
      601 gcgttttaaa agccttaaaa acttaccgtg tcttagatat gtatttggtt ggtgttaagc
      661 ctcagtacca aaaactcggt gttaatgccc ttattttatt tgaaggcatc aaatcagcaa
      721 ttaaatgcgg tgtgaagttt gcggaaacgg gaccggagtt agaaacaaac ttggaagttc
      781 gttcactgtg gcgggaattt gaaactcgtc aacaccgtcg tcgtcgttgc tatcttcgtg
      841 atttaacctc gtcgtcctaa ctgctcggat tttggttttt ttttggaatt taagtttctt
      901 ttatcggatt gactctgggc tgtttttgct tcatatttgg taatttgttg taattgacta
      961 tccaatgttg taatactacc gcgctttatt tcgcgataaa tagaggaacg atgtttccca
     1021 agttgtttag caatttcagt tatagagatg ccttgtttta aaagaacttc aatagtgatt
     1081 cgatcagtac tagtgagttg tttgaaattg gcatgattgg cggtgccatt taagttgttc
     1141 atataattcc tcctaaattt aatggtggta gtggcaaaat tcattaccac ttttctttct
     1201 tttttctcga catttcccaa aaacaaaaag gattttgcca tgggaattat agcataattc
     1261 atagataatt taaagtcttt gaaattttcc tacacgagtt gttgttgatg actttaacta
     1321 tttaatgagc tttttagttg cttgtttaac gcatttaggc ttggaagatg cccctgggaa
     1381 aatagcaaat attttgattg actttttcaa gcgaaaagta tataatatgg atgttgtgaa
     1441 atcgcaaccg ctcaaaacag gttttaagtg agtaaatcca cctagatggc gtgacttatt
     1501 aaatgaggag gtgcgaagat gatgtatgca attattgaaa ctggtggcaa acaatatcgc
     1561 gtaacaacaa atgatgttgt ttatgtggaa aaactcaatg ctgcagaaaa tgaagaaatt
     1621 gttttcgata aagtattaat ggttgatggt aaattcggtt cgccgtatct tgaaggagct
     1681 aaagtgaccg ctcgggttga gaaaaatggc aagaacaaaa agattatcgt tttcaaatac
     1741 aaagcgaaga agaactatca taaaaa
//
LOCUS       DAOSGN010000202         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_68874_length_1760_cov_2.537243, whole genome shotgun sequence.
ACCESSION   DAOSGN010000202 DAOSGN010000000
VERSION     DAOSGN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68874_length_1760_cov_2.537243"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PK087_03285"
     CDS             complement(<1..951)
                     /locus_tag="PK087_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOH18330.1"
                     /translation="MVLALILFACTYVLLLALPKYRAYVALTSAAVFIILGIIPLNKV
                     LGAIDWNVIMMIFGTMGVVGLFIESKMPSRLADFIIAKMPNVKWTIFILALFAGLISA
                     FIDNVATVLMIAPVALNIAKKLEISPVGMVIAIAVSSNLQGAATLVGDTTSILLGGEA
                     NMNFLDFFFFKGKVGIFWVVQAGALVSALILLWIFRKETAKVEEVQKTEVKDYFPTVL
                     LLGVIVLLIIASFFPKLSAATGGLINGIICVSLFIVGITRKIIVKREIKPFVEALKEI
                     DYTTLLLLIGLFVVIGGITEAGVIDKISQLFVKASGNNLFL"
     gene            complement(1369..>1760)
                     /locus_tag="PK087_03290"
     CDS             complement(1369..>1760)
                     /locus_tag="PK087_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOH18331.1"
                     /translation="TDNPQSSEYWRQLLSKYPAAFLYRVFDAMKLSKILVQEWLEKYM
                     FKDENKTNIIKEIVKKLNMNAKSHARHFSLQYCIDMGLKIVRLEDDAILQDLVLSIYH
                     ASTITISSTRAIKLIENQEGRALIRQI"
BASE COUNT          638 a          345 c          272 g          505 t
ORIGIN      
        1 caagaataaa ttattaccgc tggccttcac aaacaattgg ctaattttat caatgactcc
       61 ggcttcagta atcccaccaa tcacaacaaa taaaccgatt aataataata aggtcgtata
      121 atcaatttct tttaaagcct ccacaaaggg ttttatttct ctcttcacaa ttattttacg
      181 ggtaatcccg acaatgaata aggaaacaca gataatcccg ttaatcaatc ctccggttgc
      241 tgcggaaagt ttggggaaga aagaagcaat aatcaacaac acaatcacac caagtaataa
      301 aacagttgga aaataatctt taacttcggt tttttggact tcttccactt tagcggtttc
      361 ttttcggaaa atccaaagta agattaaagc ggaaaccaag gctccggctt gcacaaccca
      421 gaaaatccca accttccctt taaaaaagaa aaagtctaaa aagttcatgt tcgcctcacc
      481 acccaacaaa atggaggtag tgtcaccaac caaggtggca gcgccttgca aattagagga
      541 tacggcaatg gcaataacca tgcccaccgg cgaaatctct aatttcttgg caatgtttaa
      601 tgcgaccgga gcaatcatta agactgtagc cacattgtca ataaaggcgg agattaagcc
      661 cgcaaagagc gctaaaataa aaatcgtcca tttaacattc ggcattttcg caataataaa
      721 atccgccagc cgcgagggca ttttcgattc tataaacaat cccacaacgc ccatcgtccc
      781 aaagatcatc ataatgacat tccaatcaat ggctcccaaa actttattaa ggggaataat
      841 tcctaaaata ataaacaccg ccgccgaagt aagggccaca taagcgcggt atttaggaag
      901 ggccaacaat aacacataag tacatgcaaa taagattagc gctaaaacca ttctgtcacc
      961 tcataaaaac tgttcttatt gtaacataaa gccctttaaa ctgcaaccat tggtctttag
     1021 aatccacctt agaagctaca aaaatggcat tttagaactt acatatgatt gtttacaagg
     1081 ctaaattaat tagccaagca tgttaaagag atacttaaga gaaccaaaac aagtgaaatc
     1141 tttgaataat aaccaatggt tttattaaaa tgaaaaacta tgcaaataat aaaaacaatt
     1201 tgattcggat agacaaattt aataacaata tgacaaaacc caccgcatgt aaagttgcag
     1261 tgggtttgat cattttatag tttattataa aaattagtgc tttttgacca taaaaccaca
     1321 aatttttaaa gaagttaatt gtcacacaac aatattgtaa atatttgatt aaatttgacg
     1381 aatcagagct cttccttctt ggttttcaat taatttaatt gctcttgtag atgaaatggt
     1441 aattgtggaa gcatgataga tgctcaacac taaatcttgt aatatcgcgt catcttctaa
     1501 acgaacaatt tttaatccca tatctataca atattgcaac gaaaaatgtc tagcatggga
     1561 tttggcattc atatttaatt tttttacaat ttctttgatg atgttagtct tgttttcgtc
     1621 tttaaacata tacttttcta accattcttg tacaagaatt tttgaaagtt tcatagcatc
     1681 aaacactcga tataaaaaag ctgcggggta tttcgacaac aattgacgcc aatattcaga
     1741 agactgagga ttatcagtta
//
LOCUS       DAOSGN010000203         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_69468_length_1749_cov_3.262102, whole genome shotgun sequence.
ACCESSION   DAOSGN010000203 DAOSGN010000000
VERSION     DAOSGN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69468_length_1749_cov_3.262102"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..693)
                     /locus_tag="PK087_03295"
     CDS             complement(181..693)
                     /locus_tag="PK087_03295"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOH18332.1"
                     /translation="MDYKKYIATIKDFPTEGILFRDITPLMADGAAFSHAVNELKNFA
                     VEQKAEVIVGPESRGFIFGCPIAYELGIGFIPIRKPNKLPRETVSISYQLEYGHNTLC
                     MHKDAITPNKRVLIIDDLLATGGTVKATIELVEQLGGIVVGCGFLIELEYLKGRDLLK
                     KYDLKTLIKY"
     gene            838..1665
                     /locus_tag="PK087_03300"
     CDS             838..1665
                     /locus_tag="PK087_03300"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH18333.1"
                     /translation="MEPLAYKLRPQTINDIFGQTHLVGPNGVIRKMINNRLLPSIILF
                     GNPGTGKTTIALAVSLDLGLPFHTFNASIDNKARLKEIIDTLSSDHQTIIIIDEIHRM
                     KKDIQDYLLPFVENGKITMIGITTINPYHSVNPAVRSRCLILKLNPLTNDDLRAILSK
                     ACSQLEPILTIDEQATEYLVQMANGDARALINMIEGLYFSKNDNQKIDLEYAKTIIQK
                     PAINIDKSDDNYYDTLSGLHKSIRGSDVDASLHYLAKLLAAEDFLPLVRPDFATNKQ"
BASE COUNT          577 a          340 c          280 g          552 t
ORIGIN      
        1 gatccgaaga ttttcgaaat tggccttcat gcttaaggtg tgcgaattca taagcacttt
       61 gaataagctc aagatttttg gaattatgta agtattcttg cgctttgtta agcacatcgg
      121 caatcgtctt ctttgcttcc ataaaacaac ctcctttcaa aaacggtttt taaaaaataa
      181 ttaatatttg attaaagttt ttaaatcata tttttttaaa aggtcgcgcc cttttaaata
      241 ctcaagttca attaaaaaac cacatccgac gacgattcca ccaagctgtt cgactagttc
      301 aatcgtagct ttaaccgtcc ctccggttgc caataaatca tcaatgatta aaactcgttt
      361 attgggcgta atagcatctt tatgcatgca aagggtatta tgaccatatt ctaattggta
      421 agaaatggaa accgtttccc ttggtaattt attaggtttg cggataggga taaagccaat
      481 tcccagttca taagcaattg ggcagccaaa aatgaatcct cgggattcgg ggccaacaat
      541 tacttccgct ttttgttcga cagcaaaatt ctttaattca ttgacagcat gactaaaagc
      601 tgcaccatcc gccattaatg gggtaatgtc tcgaaataaa atcccttctg tcgggaaatc
      661 cttgattgtc gcaatgtatt tcttataatc catagtcctc tccttaatat gaatatgata
      721 atattatacc aaaaaattaa taaaaacacc agttgaagat aaaaaatcgt taaattttaa
      781 aactcgctta actttactaa tcttagccct tatgttaaaa taaacccggg tgataacatg
      841 gaaccattag catataaact tcgtccacaa accatcaatg acatttttgg ccaaactcat
      901 ttagttggcc ccaatggggt cattagaaaa atgattaaca accgcctttt accttcaata
      961 atcttgtttg ggaatccggg caccgggaaa accactattg ccttagccgt aagccttgac
     1021 ttgggcttac cttttcatac tttcaatgct tccattgata acaaagcacg attaaaagaa
     1081 ataatagata ccctttcttc cgaccatcaa acaattataa ttattgatga aattcatcga
     1141 atgaaaaaag atattcagga ttatttactg ccgtttgtgg aaaacggcaa aatcacgatg
     1201 atcggaatta ccacgattaa cccctatcat tccgtcaacc ccgctgttcg ttctcgttgt
     1261 ttgattttaa aattaaatcc cctaactaat gatgatttac gagccatctt aagtaaagct
     1321 tgttcacaac tagagcccat cttaacaatt gatgagcaag cgacggaata cttagtacaa
     1381 atggctaacg gtgatgctag agccttaatt aatatgattg aaggtttata tttttctaaa
     1441 aacgataatc aaaaaattga tttggaatat gcaaaaacca ttatccaaaa acctgccatc
     1501 aatatcgata aaagtgacga taattattat gatactttaa gtggtttgca taaatcgatt
     1561 cgaggcagtg atgtggatgc cagtttgcat tatctagcta aattgctggc agcggaagat
     1621 tttttaccgc tagttcgtcc cgactttgcc accaacaaac aataaaatcg ttgtagaatt
     1681 atacacttag attcatttca aatattacga atattagcct tattgagccc aaaataaggc
     1741 tttttttgt
//
LOCUS       DAOSGN010000204         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_69542_length_1748_cov_2.658594, whole genome shotgun sequence.
ACCESSION   DAOSGN010000204 DAOSGN010000000
VERSION     DAOSGN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69542_length_1748_cov_2.658594"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1581)
                     /locus_tag="PK087_03305"
     CDS             complement(211..1581)
                     /locus_tag="PK087_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH18334.1"
                     /translation="MLKRKVYDEIVEWDKNADNKVLLIDGPRQVGKSYIIRAFGKKHY
                     PNYIEINMLSSLEAQTLFKEIKSVDDFMLKLSLLTSVPIEEGKTFIFLDEIQMIKEND
                     MITFSKFMATQTKYRFVLSGSLLGVELQNVQSWPTGYMKIIKMHPLDFEEFMWAFNTN
                     EQIINHLRTSFQELTPVDSFIHEKIMDLFRLYLIIGGMPQVVVTYLETKNMQKVNETI
                     NDINMLYKKDISQYSDSNKLFIEEIYDLIPSELNSKNKRFILKNLNENFRFRKYEQSF
                     IWLAKAGVTIPVYNVDKPVIPLLISKKRTLFKLFHIDCGMLINMSMDPSIQIKILAKE
                     KAINFGAYYENVVAKELYAHGFERLYFWNSKTQGEVDFLIENEGEIIPIEVKSGKDYE
                     RHNALSNLLGNPQFAIKKAYVFQNENVMVKENRLYYPIYMITFLKKSSKLPFETIDFD
                     FSSIQN"
BASE COUNT          569 a          311 c          241 g          627 t
ORIGIN      
        1 aaaaacaaga aagcgaaata tttaattact aatctaacac acaagaatat gtaacggaag
       61 atgaacgaat tgaaataatc tcgaaaagaa tactaaataa acatcttaat gcttttaaag
      121 aactggcaaa ataaagcaat caagttagtt aacatatgcc cgattaaaaa caccgataat
      181 tttggataaa gtttttatcg gtgttttctt ttaattctgg atactggaaa aatcaaaatc
      241 aattgtttca aaaggaagtt tgctactttt ctttaaaaaa gtaatcatat aaataggata
      301 ataaaggcga ttttctttaa ccatcacatt ctcattttga aaaacatatg cttttttgat
      361 agcaaattgg ggatttccca acagatttga taaggcatta tggcgttcat aatctttgcc
      421 cgattttact tcaatgggaa taatttctcc ttcgttctca attaaaaaat cgacttcccc
      481 ttgagtttta ctattccaaa aatacaagcg ttcaaaacca tgagcatata attctttcgc
      541 aacgacattt tcataatatg ccccaaaatt aatcgctttt tctttggcta atatcttgat
      601 ttggatgctc ggatccatgc tcatattaat gagcattcca cagtcaatgt gaaacagttt
      661 aaacaaagtt cgctttttgc tgatgagcaa aggaataaca ggcttatcaa cattgtagac
      721 cggaatagta acaccggctt tcgccaacca aataaaactt tgttcatatt ttctaaatct
      781 aaagttttca tttaaatttt taagaataaa acgtttgttt ttactattta attccgaagg
      841 aattaaatca taaatctctt caataaagag tttattacta tcactgtatt gactgatgtc
      901 ttttttatac agcatattga tatcattgat ggtctcatta actttctgca tatttttggt
      961 ttccaaataa gttacgacta cttgcggcat acccccgata attaaatata gcctaaacaa
     1021 atccattatt ttttcatgaa tgaaagaatc aacaggagtc aattcttgaa aagatgtgcg
     1081 tagatggtta ataatctgtt cgttagtatt aaaagcccac ataaattctt caaaatccaa
     1141 aggatgcatt ttgatgattt tcatatatcc cgtcggccat gactgaacat tttgcaattc
     1201 aactcctaat agtgaaccac ttaagacaaa ccggtattta gtttgcgtcg ccataaactt
     1261 actaaaagtt atcatgtcat tttccttaat catttgaatc tcatcaagaa aaataaaggt
     1321 ttttccttct tcaatcggca cggatgtcaa caaggataac ttgagcatga aatcatcaac
     1381 gctttttatt tccttaaaaa gagtttgcgc ttctagtgat gataacatat tgatttcaat
     1441 ataattggga taatgttttt ttccaaaagc gcggataatg taactttttc ccacttgtcg
     1501 cgggccgtca attaacaaaa ctttgttgtc agcattttta tcccattcca caatctcatc
     1561 ataaactttt ctttttaaca tgatatccct ccgtttacca tattttatca cacacttgcc
     1621 gtttttccaa gtcttttttt cgcttttttg cagtttttcc aagtcttttt ttcgcttttt
     1681 ttgcagtttt tccaaggtgt ttttcttggt ttttgcagtt tttccaagaa aatagccatc
     1741 ttgattcc
//
LOCUS       DAOSGN010000205         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_69661_length_1746_cov_2.785925, whole genome shotgun sequence.
ACCESSION   DAOSGN010000205 DAOSGN010000000
VERSION     DAOSGN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69661_length_1746_cov_2.785925"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..333)
                     /locus_tag="PK087_03310"
     CDS             complement(193..333)
                     /locus_tag="PK087_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18335.1"
                     /translation="MLRHLEINNHEGKTLRGYLTTPDRPFSLTVVFFHGFTGPLNGGS
                     IR"
     gene            complement(326..1237)
                     /gene="murQ"
                     /locus_tag="PK087_03315"
     CDS             complement(326..1237)
                     /gene="murQ"
                     /locus_tag="PK087_03315"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOH18336.1"
                     /translation="MSVDLKKIGTEQRNTNTMDIDSLTTYEILEKINQEDQLVAGAVQ
                     KALPQIAPLVDRIVENFKKGGRLIYLGAGTSGRIGIIDAVECRPTFGVSEQMVMCLMA
                     GGEKAFVRSVEGAEDSGELAVADLKNAHLNALDVVVGIAASGRTPYVLGGIHYAHEIG
                     CITGGITTSPHSPLAALVDYPIEAITGPEALTGSTRMKSGTAQKMICNMLSTASMIKM
                     GKVYENLMVDVLATNEKLVSRAHNIIQSATDCSLADAQAAFLKYGSAKKAIFALLSGV
                     EEQAEVNSWLQLGQDNIREALRLFKHA"
     gene            complement(1239..>1746)
                     /locus_tag="PK087_03320"
     CDS             complement(1239..>1746)
                     /locus_tag="PK087_03320"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOH18337.1"
                     /translation="QRPYDDGGKVALSGMVIIPIYEALIKDPFFSKKPPKSTGRELYN
                     AQAMENMAEQYGFSHYAKEDIITTVSEVTVYGITHNIALYTPNFDRIVVSGGGSHNHY
                     LLSHLQDILNKPVLTQEDLGFSSDAKEAVAFAVLGYLTLNNQPGNVISATGAKRPVVL
                     GNIAPRKE"
BASE COUNT          468 a          397 c          407 g          474 t
ORIGIN      
        1 ggaaaagacg gtcttggaag ggtcaaagcg gcggggcaaa aagacggctc ggagttattt
       61 cttgaccttt acgcaatctg tttataggat ttgaacaaat cgttcacgga ttatgaacaa
      121 aaaggctttc aacagggaca ttgcggacgc tgaggaggcg caaaaagcgc ttgaaccggc
      181 gtccggatct tttcagcgga tagaaccacc attcaggggg cccgtaaaac catgaaaaaa
      241 gacgacagtt aacgaaaagg gacggtcggg tgtcgttaaa taaccccgta aggtttttcc
      301 ttcgtggtta tttatttcca gatgtctaag catgtttaaa taacctcagg gcttcacgaa
      361 tattatcttg ccccaactgt aaccatgaat taacttccgc ctgttcttcc actccggaaa
      421 ggagcgcaaa aatagctttt ttggcggaac cgtatttcaa aaaggcggct tgggcatcgg
      481 caagtgaaca atcagttgcc gattgaatga tgttgtgggc ccggctgact aatttttcgt
      541 tggtagcgag gacatcaacc attaagtttt cataaacttt gcccatttta atcatggaag
      601 cggtggacag catattgcaa atcatttttt gggcggtccc tgatttcatc cgcgttgatc
      661 cggttaaagc ttccggtccg gtaatcgctt caatcggata atcaaccaaa gcggctaagg
      721 ggctgtgtgg tgaagtggta atcccgccag taatacagcc gatttcatga gcatagtgga
      781 ttccccctaa gacatagggt gtgcgtccgc tggccgcaat cccgacaaca acatcaagtg
      841 catttaagtg ggcgtttttt aaatctgcaa ccgctaattc cccactatct tccgcacctt
      901 cgacggatct aacaaaggct ttttcaccgc ccgccattaa gcacatcacc atttgctcgg
      961 aaacaccaaa agtcggtcga cattcaacgg catcgataat accgatgcga ccactggtgc
     1021 cggcccctaa ataaatcaag cgtccgcctt ttttaaagtt ctccacaatc cgatcaacca
     1081 aaggggcaat ctgcggtaag gccttttgga cggccccggc cacgagctga tcttcttgat
     1141 taatcttttc caagatttca taagttgtaa gggagtcaat atccatggtg ttggtattcc
     1201 gttgttcggt gccaattttc tttaaatcaa cgctcatatt attcctttct cggggcaatg
     1261 ttaccaagga caaccggacg cttggcgccg gtcgctgaga taacatttcc cggttggttg
     1321 tttaaggtta agtagcctaa aaccgcaaaa gcgacggctt ctttcgcatc actcgaaaac
     1381 cccaagtctt cttgagtgag gaccggttta tttaagatat cttgcaagtg gcttaaaagg
     1441 tagtgattgt gactgccgcc gccactaacg acaatacggt cgaaatttgg ggtgtataaa
     1501 gcgatgttat gggtgattcc ataaacagtt acttcagaaa cagttgtaat aatatcttct
     1561 ttggcatagt gcgaaaaacc atattgttca gccatgtttt ccatggcttg cgcattatag
     1621 agttcccgtc cagttgattt tggcggtttt ttggaaaaga agggatcctt aattaaagct
     1681 tcataaattg ggataatgac catcccggat agggcgacct tgccaccatc atcataggga
     1741 cgctga
//
LOCUS       DAOSGN010000206         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_70260_length_1736_cov_1.618084, whole genome shotgun sequence.
ACCESSION   DAOSGN010000206 DAOSGN010000000
VERSION     DAOSGN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70260_length_1736_cov_1.618084"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /gene="ffh"
                     /locus_tag="PK087_03325"
     CDS             complement(<1..1074)
                     /gene="ffh"
                     /locus_tag="PK087_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOH18338.1"
                     /translation="MPFESLSERIQMSLRRITGRGKLNENDIEEMMKEVRLSLLEADV
                     NYKVVKSFTAELKQKALGEKILKSLTPGEMVVKVVHDELKRLMGDEAVLVNYRSSGPS
                     VFMLVGLQGSGKTTQVGKLAQFLRKKDGKKPLLIAGDIYRPAAINQLETIGRQLGIEV
                     FQMGTTTKVVDIVTKGIAYAKSKSYDLVIIDTAGRLHINEEMMQELNDVQKVAKPDEI
                     LLVIDAMMGQDALNVITTFNEKLPITGCILTKLDGDSRGGAALSVRYLTKIPIKFAGM
                     GEKLDQIEIFHPDRMAGRILGMGDIVTLVEKASENIDEAEAMKLAERMQKGLFNYNDF
                     IKQIKWIRRIGSLKGILGMIPDLG"
     gene            complement(1083..1403)
                     /locus_tag="PK087_03330"
     CDS             complement(1083..1403)
                     /locus_tag="PK087_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18339.1"
                     /translation="MEIFEKHHHYILLFDFYGAILTKKQQDYFRDYYFKDASLAEIAA
                     QNQISRAAVFDQLHRVHQLLDEYEAKLKLFEKFQLRHSLYEQLAKTADSQVLAIIEQL
                     KEIE"
     gene            complement(1434..1736)
                     /locus_tag="PK087_03335"
                     /pseudo
     CDS             complement(1434..1736)
                     /locus_tag="PK087_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016111220.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
BASE COUNT          499 a          387 c          291 g          559 t
ORIGIN      
        1 tccgaggtcc ggaatcattc ctaaaatgcc ttttaaagaa ccgattcttc taatccactt
       61 gatttgctta atgaaatcat tgtaattaaa caatcctttt tgcatgcgtt cggccaattt
      121 catggcttca gcttcatcga tgttttcgga agctttttcc accaaggtga caatatcccc
      181 catgccgaga atccgtcccg ccatccgatc gggatgaaaa atttcaattt ggtctaattt
      241 ttcccccatt cccgcaaact taatcggaat cttcgttaaa taacgaacgg ataaggcggc
      301 accaccgcgg gaatcaccat ctaacttggt taggatgcat cccgttatcg gcaatttttc
      361 attaaaagtc gtaatgacat tgagggcatc ttgccccatc atggcatcaa tgactaataa
      421 aatttcatcg ggtttagcta ctttttgcac atcgttgagt tcttgcatca tttcttcgtt
      481 gatatgcaag cgtccggccg tatcaataat cactaaatca taacttttac ttttcgcata
      541 ggcaatccct ttggtaacaa tgtccacaac tttggtcgtt gttcccattt gaaaaacttc
      601 aatccccagt tggcgtccaa tcgtttctaa ttggttaata gccgccggac gataaatatc
      661 accggcaatc aaaagaggtt ttttgccatc tttttttctt aaaaattggg ctagttttcc
      721 tacttgcgtg gtcttccccg agccttgcaa acccaccaac ataaaaacgg aaggcccgct
      781 ggagcgatag ttaaccaaaa cggcttcatc acccattagg cgctttaact cgtcatgaac
      841 gactttcaca accatttcgc ccggagtaag cgattttaaa atcttttcgc ccaaagcttt
      901 ttgctttaat tcggcagtaa aactcttaac aactttatag ttgacatccg cttccaacaa
      961 ggaaaggcgg acttctttca tcatttcttc aatatcattt tcgtttaatt taccacggcc
     1021 ggtaatccgg cgtaaggaca tttggattct ttcagataaa ctttcaaaag gcatatacta
     1081 acctactcaa tttcttttaa ttgttcaata atggctaaaa cttgggaatc agccgttttc
     1141 gctaattgct catagagaga atgacgtaac tggaattttt caaacagttt taatttggct
     1201 tcatattcat ctaataattg atgaacgcga tgtagttgat caaagaccgc agcacggcta
     1261 atttgatttt gagcggcaat ttccgccaag gaagcgtctt taaaatagta atcacgaaaa
     1321 taatcttgct gcttctttgt tagaatggca ccataaaaat caaacaaaag aatataatga
     1381 tgatgttttt cgaaaatttc catcttttat tcctcatcta tcatttccgc aaactagcca
     1441 tacacatatt tttctaaatc aaaataatcg atatcttcta atttttcccc taaacaaaca
     1501 aatttaatgg gaatggaata tttttcacga attgctaaaa cgatgccgtc cttagtcgtt
     1561 cgtcgagctt ggttaaaaca atgtccgaga cttgcgtagc ttcaataaac gctttggctt
     1621 gatttaaacc gttttgcccg gtggtagcat caatcactaa aagtgtatcc tgaggagcat
     1681 caggaatttc gcgagcaatc actcgtttga ttttttccaa ttccttcatt aaattg
//
LOCUS       DAOSGN010000207         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_70369_length_1734_cov_2.318046, whole genome shotgun sequence.
ACCESSION   DAOSGN010000207 DAOSGN010000000
VERSION     DAOSGN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70369_length_1734_cov_2.318046"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PK087_03340"
     CDS             <1..574
                     /locus_tag="PK087_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOH18340.1"
                     /translation="EIDENDSKSQNDFGKNIIPRMLADGKRLFAYNFNGYWKDVGTID
                     SLWEANMDLLGEKPRFDLFDPNWKIYARNDPFPPQYIMEGAVITNSLITVGCHIEGTV
                     INSLLSHNVKVGKGAIVMDSVIFADTSIEEGASVQYSIVDERVSLKSHCHVGSSKGQQ
                     GKITVISSDVIVDQGMVVQAGEMIEKDVKR"
     gene            578..1690
                     /gene="glgD"
                     /locus_tag="PK087_03345"
     CDS             578..1690
                     /gene="glgD"
                     /locus_tag="PK087_03345"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOH18341.1"
                     /translation="MATLGLIFSNIHDKEIFEVTKKRTIASAPIGGRYRLIDFTLSNM
                     VNNGISHIGVVTKNNYQSLMDHVGSGKEWDLARKNGGLVILPPYGASQDLYNSRLEAL
                     KSIINFIKFSHADYIVLADCYQVCNIDYKPIFEQHFETKADITCVYRESNITADDYSP
                     VVMLDVNEDKRIVGMDIQKSFLGKAKVSIDTWIIKKELLQTLVEDAIARNLKSFNRDI
                     LRENLNHLKICGYPFRGFFGNISSLKSYFQVNMSLLKADIRNELFNIPNRAIYTKVRD
                     SAPTKYGVNAKIVNSLIADGCIINGRVENSVIFRGCHVNSGAVVKNSILMQGTLISED
                     SHLDYVVTDKNVKIIHEKQMQGNLKKLLYVEKDGVR"
BASE COUNT          554 a          252 c          362 g          566 t
ORIGIN      
        1 agaaatcgat gaaaacgatt cgaaatcaca aaacgatttt gggaaaaata ttattccccg
       61 aatgttagct gatggaaaac gcttatttgc atataatttt aatggttatt ggaaagatgt
      121 agggacgatt gatagtcttt gggaagctaa tatggatttg ttgggggaaa aaccgcgttt
      181 tgatttattt gatcccaatt ggaaaattta tgcccgtaat gatccttttc cgccccaata
      241 tattatggaa ggtgcagtta tcactaattc tttgataacg gttggttgcc atattgaggg
      301 aacggttatt aattctttgc tttcccataa tgtaaaagtc ggtaaaggag caattgtaat
      361 ggacagtgtc atctttgctg atacaagcat tgaagaagga gcatcggttc agtacagcat
      421 tgtggatgaa agagtaagtt taaaaagtca ttgccatgta ggttcttcta aaggtcaaca
      481 aggaaaaatt acggtcatct cctccgatgt aatcgttgat caagggatgg ttgtccaagc
      541 tggcgaaatg attgagaaag atgttaagag gtaatatatg gcaacactag gtttaatatt
      601 ttctaacatt catgataaag aaatctttga agttaccaaa aaacgcacga ttgcttcggc
      661 tccaattggt ggaaggtatc gcttaattga ttttacatta tcgaatatgg ttaacaatgg
      721 catttcccac attggtgtcg tcaccaaaaa taattatcaa tcattgatgg atcatgtcgg
      781 cagtggtaag gagtgggatt tagcgcgtaa aaatggcggt ttggtgatcc ttccgcccta
      841 tggtgctagt caagatttat ataactcgcg tttggaagct ttaaagagca tcattaattt
      901 tattaaattt tcccatgctg attatattgt tttagcggat tgctatcagg tttgcaacat
      961 tgattataaa cccatttttg aacaacattt tgaaacaaaa gctgacatta cttgtgttta
     1021 ccgtgaaagc aatataactg ctgatgacta tagtcctgtt gtgatgcttg atgttaatga
     1081 agataaacgc atcgtcggga tggatattca aaaaagtttt ctaggcaaag ccaaggtatc
     1141 tattgatact tggattatca aaaaagagct tcttcaaaca ttagttgaag atgctattgc
     1201 gaggaattta aagagtttta accgggatat tttaagagaa aaccttaatc acttgaaaat
     1261 ttgcggttat ccgtttaggg gctttttcgg gaacatcagt tctttaaaat cttattttca
     1321 agttaatatg agtcttctca aagctgatat tcgtaatgaa ttattcaata ttcctaatcg
     1381 ggccatttat accaaagttc gcgattcggc ccctacaaaa tatggtgtca atgcgaaaat
     1441 tgttaactcg ttaattgctg atggatgtat cattaatggg cgagtggaaa attctgttat
     1501 tttccgtggt tgtcatgtta attcgggagc agttgtcaag aattccattt taatgcaagg
     1561 aactttaatt agcgaagata gccatttaga ttatgtcgtt accgataaaa atgtaaaaat
     1621 aatccatgaa aaacaaatgc aaggaaatct aaaaaaacta ctttatgtgg agaaggatgg
     1681 tgttcgatga aacaaaaaaa tattcttttt gtggttgcgg aatgtgctaa tttt
//
LOCUS       DAOSGN010000208         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_70404_length_1733_cov_3.483313, whole genome shotgun sequence.
ACCESSION   DAOSGN010000208 DAOSGN010000000
VERSION     DAOSGN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70404_length_1733_cov_3.483313"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PK087_03350"
     CDS             <1..313
                     /locus_tag="PK087_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOH18342.1"
                     /translation="MYHHILDPRTGFPINNGVISVTVICQESIVGDGFSTSLFTLGLE
                     KGMEVVNALDYLEAVWVVEQGQKKAVYISKGLKDIFVFNEAVATLGYVYQGVYNENFG
                     N"
     gene            294..1109
                     /gene="sufC"
                     /locus_tag="PK087_03355"
     CDS             294..1109
                     /gene="sufC"
                     /locus_tag="PK087_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOH18343.1"
                     /translation="MKILEINNLHSAVEDTEILKGVNLSLKTGEIHAIMGPNGTGKST
                     LAATIMGHYKYVVTKGSITLDGEDLLALSVDERSRKGIFLAMQYPNEISGITNADFIK
                     TALQARMEAGQHLSLFKFIREMDKAVSDLKMHHDLPHRYLNEGFSGGEKKRNEILQMK
                     MLKPYFAILDEIDSGLDIDALKIVGENINAMRGENFGALVITHYQRLLDYIDVDYVHI
                     MMNGQIVRSGGKDLIARIDAEGYDWLRAELDLKDENEKMVAVLGSCGFKKVQE"
     gene            1115..>1733
                     /locus_tag="PK087_03360"
     CDS             1115..>1733
                     /locus_tag="PK087_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOH18344.1"
                     /translation="METLKTKLIDGKEAFYLLKNGRVWKSFDGSLELPSFKAGNYKIV
                     HILDESIDLSYHFEKDADVSLQEVLIIEGQVNVNKTVVLEDNASVHHLCFMNSKRPGK
                     VSITMQTKLLKRAILCLSQLFIGQTPFNFHQETKLAGELASVHHKNVIINSSGYEQDF
                     HLSVSHEQAKTLSRLQNYGIANHQSLLKIFTNGIIAKKAIASDLKQ"
BASE COUNT          539 a          291 c          387 g          516 t
ORIGIN      
        1 gatgtatcat cacattttag acccgcgcac gggctttccg attaacaacg gcgttatttc
       61 ggtaacggtc atttgtcaag aatcgattgt cggtgatggt ttttccacaa gcctctttac
      121 tttaggttta gaaaaaggca tggaagtcgt caatgcctta gattatcttg aagccgtttg
      181 ggtggttgaa caagggcaaa agaaagccgt ttatatttct aagggcttaa aagatatttt
      241 tgtttttaac gaagccgtgg caacactggg ttatgtctat caaggagtat ataatgaaaa
      301 ttttggaaat taacaattta cattccgctg ttgaagatac ggagatttta aaaggagtta
      361 atctcagcct taaaactggc gaaatccatg ccatcatggg ccccaacggt accgggaaat
      421 cgactttagc agcgacgatt atggggcatt ataaatatgt agtaacaaaa ggtagtatta
      481 ctttagatgg cgaggattta ttagcgctgt ccgtggatga acgcagtcgc aaaggcattt
      541 tcttagcgat gcagtatcca aacgaaattt cggggattac caatgccgat tttattaaaa
      601 cggccttgca agcgcggatg gaagcgggac agcatctttc actgtttaag tttattcgcg
      661 aaatggataa agccgtcagc gatttaaaaa tgcatcatga tttaccgcac cgttatttaa
      721 atgaaggttt ttccggtggg gaaaagaaac gcaatgaaat tctccaaatg aaaatgttga
      781 aaccgtattt tgcgattttg gatgaaattg acagtggact ggatattgat gcactgaaaa
      841 ttgtcggcga aaacattaat gcgatgcgtg gtgaaaactt tggggcttta gtcattactc
      901 attaccaacg tttgctcgac tacatcgatg ttgattatgt tcacattatg atgaatggcc
      961 aaattgttag gagcggtggc aaggatttga ttgcgagaat tgatgcggaa ggctatgatt
     1021 ggttgcgggc ggaactcgac ttaaaagatg aaaatgaaaa aatggtggca gttttaggct
     1081 cgtgcggctt taaaaaagta caggaataag gatcatggaa actttaaaaa ccaagttaat
     1141 tgatggtaaa gaagcttttt atcttttaaa aaacggtcgg gtttggaaaa gctttgacgg
     1201 ttctttagaa ttaccgtctt ttaaagccgg aaattataaa atcgttcata tacttgatga
     1261 atcaattgac cttagttatc attttgaaaa ggatgcggat gttagtttac aagaagtttt
     1321 aattatcgaa gggcaagtga atgtcaacaa aaccgtcgtg cttgaagata atgcttctgt
     1381 tcatcattta tgctttatga atagtaagcg tccgggtaaa gtctcaatca caatgcaaac
     1441 aaagctttta aagcgtgcta ttctttgctt gagtcaatta ttcattggcc aaacgccgtt
     1501 taattttcac caagaaacga aattggcggg tgaacttgcc agcgttcatc ataaaaatgt
     1561 catcatcaac agctcgggct atgaacagga ttttcattta tccgtcagcc atgaacaagc
     1621 gaaaaccctt tccaggttac aaaactatgg cattgctaat catcaatcct tactaaagat
     1681 ttttaccaac ggtatcattg ctaagaaggc gattgcttcc gacttgaagc aaa
//
LOCUS       DAOSGN010000209         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_70468_length_1732_cov_3.589147, whole genome shotgun sequence.
ACCESSION   DAOSGN010000209 DAOSGN010000000
VERSION     DAOSGN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70468_length_1732_cov_3.589147"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /gene="trmD"
                     /locus_tag="PK087_03365"
     CDS             <1..596
                     /gene="trmD"
                     /locus_tag="PK087_03365"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOH18345.1"
                     /translation="LQVDDYSYGGGAGMVISVEPLVACLRSIAGYEQAHKILTSPIGP
                     IYKQAKASQLAHYNHLIIICGHYEGIDERILNYVDEVVSIGDFILTGGELVAMMIVDS
                     IVRLLPDALGNNQSLTTESFQERLLEYPQYTRPECFETYRVPEVLMSGDHEKIRQYRR
                     FKALERTYRLRPDLLAEASLTEEDLKWLNKIKQGETL"
     gene            593..1045
                     /locus_tag="PK087_03370"
     CDS             593..1045
                     /locus_tag="PK087_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOH18346.1"
                     /translation="MKRLVLLLLLACLVGCTSGKSFVYHDDELAKFDKVLENIGDGHT
                     KAYDLRSEEDCLLGRIPGFFCMRTIDSSNNEKSLDTIFEHLSLLIGNDYAYLIILMDA
                     DGIDSAYLAGLLVDAGYRNVHYFASGYARYVYLKGDSFVPEVGPCENC"
     gene            1188..1535
                     /gene="rplS"
                     /locus_tag="PK087_03375"
     CDS             1188..1535
                     /gene="rplS"
                     /locus_tag="PK087_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOH18347.1"
                     /translation="MSQQLIADITSAYMKNDIPEFRPGDTVKVHVKIKEGNRERIQMF
                     EGLVIKRRGGGISETFTVRKNSYGVGVERTFPVHTPNIAKIEVLRKGLVRRAKLNYIR
                     TLSAKAARIKERK"
     gene            1610..>1732
                     /locus_tag="PK087_03380"
     CDS             1610..>1732
                     /locus_tag="PK087_03380"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOH18348.1"
                     /translation="MKKERLQKYARLAVEVGINVQEGQLLIINSPVECQEFTRLL"
BASE COUNT          507 a          299 c          401 g          525 t
ORIGIN      
        1 gtttacaggt tgatgattat tcctatgggg gcggggcggg aatggtaatc agcgtcgaac
       61 cgcttgtggc ttgtttgcgt tcaattgccg gttatgagca agcgcataaa atcctaacca
      121 gtcccatcgg tcccatttat aagcaagcaa aagcgtccca attggcccat tataatcact
      181 tgattattat ctgcggtcat tatgaaggga ttgatgagcg aattttaaat tatgtggatg
      241 aagttgtttc gattggcgat tttatcttaa ccggtggcga actcgttgcc atgatgattg
      301 tggacagtat cgtgcgctta ctgccggacg ctttagggaa taatcaatcg ctaacaacgg
      361 aaagttttca agaaaggtta ttagaatatc cgcagtacac tcggccggaa tgctttgaaa
      421 cctaccgggt tcccgaagtg ttgatgtccg gagatcatga aaaaatccgg caataccgcc
      481 ggtttaaagc tttggagcga acataccgct tacgcccgga tttattggcc gaagcgtcat
      541 tgacagagga agaccttaag tggcttaaca agattaaaca aggagaaaca ctatgaaaag
      601 attagtttta ttgttgcttc ttgcttgttt agtgggctgc acttcaggga aaagctttgt
      661 ttatcatgat gatgaactgg caaaatttga taaagtttta gaaaatatcg gtgatggtca
      721 caccaaagct tatgacctta gaagtgaaga agactgtttg ttggggcgta ttcccggttt
      781 cttttgtatg cggacgattg attcttcaaa caacgaaaaa agcctcgata ccatttttga
      841 acatttaagt ttgttgattg gtaacgatta tgcttattta attattttaa tggatgctga
      901 tggcattgac agtgcctatt tggcgggatt attagtcgat gccgggtatc gcaatgttca
      961 ttatttcgca agcggttatg ccaggtatgt ttatttaaaa ggcgacagct ttgttcccga
     1021 agtcggtccc tgtgaaaatt gttagatttt ttggaaatta cttgctttta aagggcattg
     1081 gcgttataat atacaaggct agaaacaaga acggttttta gttgatcacg atgttccgct
     1141 gctacttttt gatagttatg aacattggcc gaaggagcgt gaaaacaatg agtcaacaat
     1201 tgattgctga cattaccagc gcttacatga agaatgatat tccggaattt cgtcccggtg
     1261 acacggttaa agtccatgtc aagattaagg aaggaaatcg tgaacgaatt caaatgtttg
     1321 agggattggt aattaaacga cgaggaggcg gaattagcga aacctttacc gttcgtaaaa
     1381 actcatatgg tgttggtgtt gaaagaactt ttccggttca caccccaaat attgcgaaaa
     1441 tcgaagtttt acgaaaaggt ttagttcgcc gtgcgaaact caattatatt cgtacattgt
     1501 ccgcaaaagc agcgcgtatt aaagagcgca agtaacaaaa aagcaggaaa ctgctttttt
     1561 tgcttttatc cgctcttttt ttgtgttatc ataaacaaaa ggagcttata tgaagaaaga
     1621 aagattacaa aaatatgccc gtttagccgt tgaagtcggg attaatgtcc aagaaggaca
     1681 attattaatc attaattctc ccgtcgaatg tcaagaattt acacgccttt tg
//
LOCUS       DAOSGN010000210         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_71459_length_1716_cov_3.054786, whole genome shotgun sequence.
ACCESSION   DAOSGN010000210 DAOSGN010000000
VERSION     DAOSGN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71459_length_1716_cov_3.054786"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PK087_03385"
     CDS             complement(<1..287)
                     /locus_tag="PK087_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH18349.1"
                     /translation="MISVKNLHKYFNRNKPNEIHVINNVNLTFPKTGLVCLLGPSGSG
                     KTTLMNVISGLDRVHSGEIEFRDHKISKYHANEWDLIRNRYFGYVFQNYILL"
     gene            complement(289..>1716)
                     /locus_tag="PK087_03390"
     CDS             complement(289..>1716)
                     /locus_tag="PK087_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18350.1"
                     /translation="GKMSTIAKTFQIAIYNLLSQPKKTILLLLVAITTLFFVFINYGS
                     KLVAQNMDHYGNGLYNSYPERLVVTRKNKAPLTTTDYDYFMSQSEITKVIIPDLVVDA
                     QFHINFQIKDSYYYEDGFLNFNNDFAHQVVIGRLPQADNEIALAFNPYSIDENNLDKP
                     LNAIYDISISTANGYFQMPYKLVGIYPTTDQSQFILSEGGITKLYDLMKISLKGYQFI
                     VKDGGVEQNLPYGMISVNNSLSEHNIKISLYYKDILPGNLEQAEVSLNNVPVTITFDA
                     LTDHYNTIFVSPSFFQELKVDEDYQYTLNLKDKDQANKVINRLFDNGYYAFSPFLASK
                     VIDFDAIFSVITNIFATLGVLFSVAVTFIISYLVVRSILLSKKKDYTILRIIGLKFQE
                     LKQVAVLEIITIFLGALLILSGIYITLIIGSYEIRNLVNKTFTFWNYFSLITLSLAVM
                     ILIAWRFTRMMTKKSLFMNLKVEGE"
BASE COUNT          612 a          292 c          271 g          541 t
ORIGIN      
        1 agtaaaatgt aattttggaa aacataacca aaataacgat tacgaattaa atcccattcg
       61 ttagcatgat atttgctaat tttgtgatcc cgaaattcaa tttcgccact gtgaacacgg
      121 tctaatccgc taatcacatt cattaatgtc gtttttccac ttcccgaagg acctaataag
      181 cacaccaagc ctgtttttgg aaaagttaaa ttgacattgt tgatgacatg aatttcattg
      241 ggtttattac gattaaaata tttatgtaaa ttcttaacac taatcatctt attcaccttc
      301 aacctttaaa ttcatgaaca aactcttttt agtcatcatc cgtgtaaacc gccaagcaat
      361 taaaatcata acagccaacg aaagagtgat taatgaaaaa taattccaaa atgtaaatgt
      421 tttattaact aaattacgaa tttcatagga accaataatt aaagtaatat aaattccact
      481 taatattaat aaagctccca aaaaaatcgt gattatttcc aagacggcaa cttgttttaa
      541 ttcttgaaat tttaagccga taatcctaag aatcgtataa tctttctttt tactcaagag
      601 aattgagcga acaactaaat atgaaataat aaaggtaaca gcaacggaga ataaaacacc
      661 aagcgtggca aaaatattag ttatgacgga aaaaatggca tcaaaatcaa tgactttcga
      721 cgccaaaaat ggcgaaaaag cataataacc attatcaaaa agacggttaa tcactttgtt
      781 ggcttggtct ttatccttta aattcaaagt atattgataa tcttcatcca cttttaattc
      841 ttgaaaaaaa cttggactaa caaaaatcgt attgtaatgg tcagtaagag catcaaaagt
      901 aatggtgacg ggaacattat ttaagcttac ttccgcttgc tcaagattac cgggtaagat
      961 atctttataa tatagtgaga ttttaatatt atgctcggat agactattat tcacgctaat
     1021 catcccatag ggaaggtttt gttcaacccc accatccttg acaatgaatt gatagccttt
     1081 taacgatatt ttcataagat cgtataattt agtaatgcca ccttcactta agatgaattg
     1141 gctttgatca gtagtgggat agattcccac caatttataa ggcatttgga aatagccatt
     1201 agcggtggaa atactaatgt cataaatcgc attcaaaggc ttatcaagat tattttcatc
     1261 aatggaatag gggttaaaag ccaatgcgat ttcgttatcc gcttggggaa gtcgaccaat
     1321 taccacttga tgagcaaaat cattattaaa gttcaaaaaa ccatcctcat aataatagga
     1381 atccttgatt tgaaaattaa tatgaaattg ggcgtcaact actaaatcgg gaatgatgac
     1441 tttcgtaatt tcgctttgtg acataaagta atcataatcg gtcgttgtta ggggcgcttt
     1501 attcttccta gtaacaacca gccgttccgg ataactatta tacaggccat taccataatg
     1561 gtccatattt tgggctacta acttggaccc ataattgata aaaacaaaaa agagtgtcgt
     1621 aattgctacg agcaataata aaatggtttt tttaggttgg cttaaaaggt tataaatcgc
     1681 aatttggaag gtcttggcaa ttgtcgacat tttccc
//
LOCUS       DAOSGN010000211         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_71529_length_1715_cov_2.940964, whole genome shotgun sequence.
ACCESSION   DAOSGN010000211 DAOSGN010000000
VERSION     DAOSGN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71529_length_1715_cov_2.940964"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PK087_03395"
     CDS             <1..534
                     /locus_tag="PK087_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012620583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH18351.1"
                     /translation="DINEYSKASVRSQMGVVLQTTYLFKGTVLDNIKYGHPKASFEQV
                     VAAAQTAQVHDIIERLPRKYQTKVKEGGRNFSHGERQLIAIARAILGNPPIIVLDEAT
                     SSVDTRTEAKIQKSIGFLVKNRTSFIIAHRLQTIKNADLILVIQEGKITEQGNHEQLI
                     AQKGLYYEMYALQFNKL"
     gene            546..>1715
                     /locus_tag="PK087_03400"
     CDS             546..>1715
                     /locus_tag="PK087_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18352.1"
                     /translation="MDISKTAFKEYTRCPRIFPLEQLYLFRLNSDIRLFDDEEKTEAR
                     EILRLMFDEETGDDLLEVSNEQMEIMLPYYRDVEKWALKIASETFLLPFPYFEKTKDQ
                     TCFAFKSQNHRYYTYLDGYYEDESQAIVIEVKATTSRKFKKLGPKIRNVDRRDCLFEK
                     QGNVFRFRQELFKAEVKKAYVKDYEKLLDRFSDVGKYVFDIAVERFFIEQSLKQQNKN
                     LNEKKRSYYLALINNDYEYLGQTLNGKTDYPFLPDGSAIIDFVDVTDITAAYQTKIGK
                     MWQALETNLMDKTLPEARCGVKCQLATSESCFFERTCWPDLFNQASFLDYMGRKTFTD
                     LNGRQYDKYDLINQGYRKFTDIPREWLSDDNHLIQRSCFDNNEVYVDKDKIKAGFS"
BASE COUNT          557 a          316 c          350 g          492 t
ORIGIN      
        1 gatatcaatg aatactccaa agcaagcgtt cgttcccaaa tgggtgtcgt cctccaaacc
       61 acttatttat ttaaaggcac ggttttagat aatattaaat acgggcatcc caaggccagc
      121 tttgaacaag tggtggcggc tgcccaaacc gcccaagttc atgacattat tgaacgttta
      181 ccgcggaaat atcaaaccaa agtgaaagaa ggcgggcgca atttttccca tggcgagcgg
      241 caattaatcg ccattgctcg ggccatttta gggaatcctc ccattattgt tttggatgaa
      301 gcgacctcga gcgttgacac gcggacggaa gccaaaattc aaaaaagcat cgggttttta
      361 gtgaaaaacc gtaccagttt catcattgct catcgtttgc aaaccatcaa gaatgcggat
      421 ttaattttag tcattcaaga gggtaaaatt accgaacagg gtaaccatga gcaactgatt
      481 gcgcaaaaag gtctttatta cgaaatgtac gctcttcaat ttaataaact ttaaaggagg
      541 aaaggatgga tatttcaaaa acagcattta aggagtacac gcgttgcccg cggatttttc
      601 cgcttgaaca attatacttg tttcgtctta attccgatat tcgcttattt gatgatgagg
      661 aaaagacgga agcacgggaa attttaagat tgatgtttga tgaagaaacc ggtgatgatc
      721 ttttagaagt ttctaatgaa caaatggaaa ttatgcttcc ttattatcgg gatgtggaaa
      781 aatgggcctt aaaaatcgct tcggaaactt ttttgcttcc ctttccttac tttgaaaaaa
      841 ccaaggacca aacctgcttt gcctttaaaa gtcaaaacca tcgctattat acttacttag
      901 acggttatta tgaagatgag tcccaagcga ttgtgattga agttaaagcg acgacttcgc
      961 gaaagtttaa aaaactggga cctaaaatta ggaatgttga ccgccgtgac tgtcttttcg
     1021 aaaagcaggg gaatgtcttt cgttttcgcc aggagctgtt taaagctgaa gtgaagaaag
     1081 cgtatgtaaa agattacgaa aaattgttgg accgcttttc ggatgtcggc aagtatgtct
     1141 ttgatattgc cgtggaacgc ttttttatcg aacaaagttt aaaacagcaa aacaaaaatc
     1201 ttaatgaaaa aaaacggtcc tattatttag ccctcattaa caatgattat gaatatttag
     1261 gacaaacctt aaatggaaaa acagattatc cgtttttgcc ggacggtagc gccatcattg
     1321 attttgtaga tgttacggac attaccgccg cttaccaaac taaaatagga aaaatgtggc
     1381 aggctttgga aacaaattta atggacaaaa cacttcccga agctcgttgc ggtgtaaaat
     1441 gtcaattagc aacatcggaa tcatgttttt ttgaaagaac ttgttggccc gatttattta
     1501 atcaagcatc atttttggat tatatgggtc gcaaaacctt caccgatttg aatggacgcc
     1561 aatatgataa atatgattta ataaaccaag gctatcggaa atttaccgat attccccgag
     1621 aatggttaag cgacgacaat cacttaattc aacgctcatg ttttgataac aatgaagtct
     1681 atgttgacaa agacaaaatt aaagccggct tttca
//
LOCUS       DAOSGN010000212         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_72829_length_1695_cov_1.906707, whole genome shotgun sequence.
ACCESSION   DAOSGN010000212 DAOSGN010000000
VERSION     DAOSGN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72829_length_1695_cov_1.906707"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK087_03405"
     CDS             complement(<1..414)
                     /locus_tag="PK087_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH18353.1"
                     /translation="MNSEKIIEVSNLHKSYKDVHAVNGINFSVKKGDLFAFLGPNGAG
                     KSTTIDILTTILEADSGRILIDGWEIGKDDFEIKKAIGVVFQDKVLDELLTVKENLLF
                     RAALYGLSAKEAEAKVQNALQMTNLEDIQNRRYKVL"
     gene            complement(407..955)
                     /locus_tag="PK087_03410"
     CDS             complement(407..955)
                     /locus_tag="PK087_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18354.1"
                     /translation="MKKIIASINRRMVIISLLLLAVFVIVILPIVNGVLLRKLHTALI
                     PDMMIFYSPTILNNLAQIYGPEGIQVYIQSKYWFDIIWPLVYWFSLTVIIGHLCNMMK
                     QKGIALFFPTLALLFDWGENTLLIIFFSRYPQSTGILAYVASVLTAGKWVSIALSLVF
                     ILMYLGRIRKKRLQKEEKNHNE"
     gene            complement(1383..>1695)
                     /locus_tag="PK087_03415"
     CDS             complement(1383..>1695)
                     /locus_tag="PK087_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOH18355.1"
                     /translation="KHPGIGYRITKASPELSSIAEYVLSHHEKWDGSGYPQGLKGQEI
                     PLLSRIISIVDAFDAMTQERSYRPTKTTEEALAEIKACAGTQFDPYLAALFIELMEED
                     A"
BASE COUNT          548 a          325 c          286 g          536 t
ORIGIN      
        1 taagacttta taacgtcggt tttgaatatc ttccaaattt gtcatttgta aagcattctg
       61 aactttggct tcggcttctt tagccgaaag accatataaa gcggctcgaa acaataaatt
      121 ttctttcacg gtcaataact catctaagac cttgtcttgg aaaacaacgc cgatggcttt
      181 ttttatctca aaatcatctt tgccgatttc ccaaccgtca attagaattc gacccgagtc
      241 cgcctctaaa atggtggtta aaatatcaat ggtggtggat ttccccgccc cattgggacc
      301 taaaaaggca aataaatcgc cttttttgac actaaaattg atgccattaa cggcatgaac
      361 atctttgtaa cttttatgca aattactaac ttcaataatt ttttcactat tcattgtggt
      421 tcttttcctc tttctgaaga cgtttttttc taattcttcc taaatacatt aatataaaaa
      481 caaggctaag ggcaatggaa acccatttgc cggccgttaa aacactggcc acataagcta
      541 aaatacccgt tgactgggga tagcgactaa aaaagatgat taacaaagta ttttcgcccc
      601 aatcgaataa gagggcgaga gtaggaaaaa acaaagcgat gcccttttgt ttcatcatat
      661 tacacaaatg accgataatg acggtcaaag aaaaccaata aacaaggggc caaataatat
      721 caaaccaata tttggattga atataaactt ggattccttc aggaccataa atttgcgcta
      781 aattgtttaa aatagtcggc gaataaaaaa tcatcatgtc ggggataagg gccgtatgga
      841 gtttccttaa caaaacccca ttaacaatcg gtaagataac aatcacaaac acggctagta
      901 aaagaaggga aatgatgacc attcgacgat ttatgctggc aattattttt ttcataattc
      961 accctttcaa cattctatta taagcttgtt attccgtcaa ccaatacgca ttaaatcacc
     1021 ttttttcttc ctctattaat caagagtgag ggcaaataaa aatccttata cggaagtaat
     1081 actattttta gttttcttgg tttgtaccca cctaataaag caaaaacgat taatcaaacg
     1141 agtgaagcga taattattcc tcgttatttt ttttccatat tccacctttt ccttgtttta
     1201 ttatcaactc gacactatta tactatatgc catacattat tgtcaagtaa aaaatattat
     1261 tattttatga ttgaaaagta atgataaaaa aaggagcagt ctagttgctc cctttattca
     1321 tggatcattt ttgcttggaa agaaagctaa agtgatattt attgaatgaa ttttggcaaa
     1381 ttttaagcat cttcttccat taattcaata aataaggcgg ctaaataagg atcaaactgc
     1441 gttcccgcac aagctttgat ttccgctaaa gcttcttccg ttgttttggt gggacggtag
     1501 gaacgctctt gggtcatggc atcgaaagca tcaacaatgc tgatgatgcg tgataagagg
     1561 ggaatttctt gaccttttaa accttgtgga tagccggaac cgtcccattt ttcatgatga
     1621 gacaaaacat actcggcaat cgaactcagt tccggtgatg ctttggtaat gcggtaacca
     1681 attcccggat gtttt
//
LOCUS       DAOSGN010000213         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_72936_length_1693_cov_2.728938, whole genome shotgun sequence.
ACCESSION   DAOSGN010000213 DAOSGN010000000
VERSION     DAOSGN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72936_length_1693_cov_2.728938"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..>1693
                     /locus_tag="PK087_03420"
     CDS             117..>1693
                     /locus_tag="PK087_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH18356.1"
                     /translation="MEESLYGIITKVTYYSEETHFGVVKVKLDYQDHRIAKYKAKLFT
                     NILTVTGNFARQPLVDEQYDFIGEFVTNQYGIQFKASSCVVRNDNTLEGVITFLSSDL
                     FPGVGKVTATKIFQALGEDCLSKIEEDQSVLNGIEGISSKQKQTIYDNLVDFQKNKQT
                     LVGLLDLGITMRTATKIMKFLGNDALEKVRTNPYLLMDMVEGFGFRRADKIALAVGFA
                     PDSPIRLQALFLFLIRQICFSTGDTYVDKDYLYQKAAEELNQEVLSTDHFHHNLEALI
                     NDRRIVIDEANNIFDLSLYNAEILLAQQVHLFLNNPNQATYSDQEIDKALEKIAIINK
                     IEYGDKQKAAIIMALKEPLSIITGGPGTGKSTIIKGLIECILQLYPSESILEKIALLA
                     PTGRAAKRLRELTGYPAMTIHKFLGYEGHGFYRFGPLAKVDAKIVIVDEVSMVDLGLA
                     ARLFGALEDDTKVVLVGDVDQLPSVGPGDVLNDLITCKEISTVRLTKIHRQAEDSSIV
                     ALAHQINSGMVPESILER"
BASE COUNT          516 a          303 c          372 g          502 t
ORIGIN      
        1 aagccgttac tccggggcaa gcggccgttt tttatgatgg tgaacaatta atcgatgggg
       61 gtacgattgc ttcagtttac atgggaaaag aattaagacc ttattaggga gtgtttatgg
      121 aagaatcatt atacgggatt attaccaaag tcacatatta tagcgaagaa acgcattttg
      181 gggttgtgaa agtcaaactc gattatcaag accatcggat tgcgaaatac aaagccaaat
      241 tattcaccaa tattttaacg gtaacgggta actttgctag acagccgctt gttgatgaac
      301 aatacgactt tataggcgaa tttgtgacca atcaatatgg gattcagttt aaagcgagtt
      361 cttgtgttgt ccgtaatgac aataccttgg agggtgttat taccttttta tcaagcgatt
      421 tgtttcccgg agtcggtaaa gtcacggcca ccaaaatatt tcaagcttta ggtgaagatt
      481 gcttaagtaa aattgaagag gatcaaagtg ttttaaacgg tattgaaggc atatcaagca
      541 aacagaaaca aaccatttat gataatcttg tcgactttca aaagaacaaa caaacattgg
      601 tgggactttt ggacttaggg ataacaatgc ggacggcgac gaaaattatg aagtttttag
      661 gaaacgacgc tttggaaaaa gtccgcacca atccatatct attaatggat atggttgaag
      721 gctttggctt tcgtcgagct gataaaattg ctttggcggt gggatttgct cccgacagcc
      781 cgattaggct tcaagcctta ttcttgtttt taattcggca gatttgtttt tctaccgggg
      841 atacttatgt tgataaagac tatttatacc aaaaagctgc agaagaatta aaccaagaag
      901 tattatcaac ggaccatttt caccataatt tagaagcttt gattaatgat cgccggattg
      961 ttattgatga agcgaataat atttttgatt taagtttgta taatgcggaa atattgctcg
     1021 ctcagcaagt tcatcttttt ttaaacaatc ccaatcaagc tacttattcc gatcaagaaa
     1081 ttgataaagc tcttgaaaag atcgccatta tcaacaaaat tgaatatggc gacaaacaaa
     1141 aagccgccat catcatggcg ctcaaagaac cgctttcgat tattaccggt ggacccggga
     1201 caggtaaatc aaccattatc aaaggcttga ttgaatgtat tttacaactc tatcccagtg
     1261 aaagtattct ggaaaaaatt gctttattag caccaaccgg gcgggcggcg aaaaggttaa
     1321 gagaactaac tggttatccg gcgatgacca tccataaatt tttaggttat gaaggtcatg
     1381 gcttttatcg ctttggtccc ttagccaaag ttgatgcgaa aatcgtgatt gttgatgaag
     1441 tatcaatggt tgatttgggt ttggcagcgc ggttgttcgg cgctttagaa gacgacacga
     1501 aagtggttct tgtcggcgat gtcgatcaac tcccttccgt tggtccgggt gatgttctaa
     1561 atgacttaat cacttgtaag gaaatctcga ccgtccgctt aaccaagatt catcgtcaag
     1621 ccgaggattc atcaattgtg gccttggctc accaaatcaa tagcggtatg gttcccgaaa
     1681 gcattttaga gcg
//
LOCUS       DAOSGN010000214         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_73023_length_1692_cov_2.152718, whole genome shotgun sequence.
ACCESSION   DAOSGN010000214 DAOSGN010000000
VERSION     DAOSGN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73023_length_1692_cov_2.152718"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PK087_03425"
     CDS             <1..773
                     /locus_tag="PK087_03425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOH18357.1"
                     /translation="DNVYHRGHLFFVYILVAAFYFIISFVVMIVKRKSIRKSNFIPLH
                     LFLIPPLIGAVLQSLYYGLLLTWQTIALSILLVFVFVQSENVVTDYLTGLYNKREFEK
                     YLSTINKSRYRFRKLGGIFFDLDNFKQINDQFGHLMGDFILREIGNILRQSFRRTDFL
                     ARIGGDEFVAFFEISHKEDIRVFQNRLLANVSELNKTNKLPFIVSISMGIDCFDAKSP
                     FSIHEFFAHLDKLLYEQKEQKPIFHLHPADGESSLKQK"
     gene            972..>1692
                     /locus_tag="PK087_03430"
     CDS             972..>1692
                     /locus_tag="PK087_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18358.1"
                     /translation="MDYKQAAKDFLENEKQFHLGVIPTEQSNPITRNLSPTIAKDTAA
                     GIKMILGADVNIPSATAKAFATKEFAQLVDDIKRVIDEKKTLLFSSVGASGRMALQLD
                     GMWRLFWTSMITKIPAKRQMFLENIERCNSFMTGGDRALVKTAENYEDYMTFGREQVK
                     EAKVGPGDVVVGLAECGLSASINGSVLEADERGCTTYYIYCNPKEILVKHLDRVRVVF
                     ERENIIKISLFVGNMAVAGSTR"
BASE COUNT          507 a          294 c          344 g          547 t
ORIGIN      
        1 aggataatgt ctatcatcgt ggtcacttgt tctttgttta tattttagtg gcggcttttt
       61 atttcatcat cagttttgtt gtaatgattg tgaaaaggaa gagcatccgc aaatccaatt
      121 ttatcccctt acatttgttc ctcattcctc ctttgattgg agctgtctta caatcgcttt
      181 attacggttt actgttaact tggcaaacga ttgctttatc cattttactg gtctttgtct
      241 ttgtacaatc ggaaaatgtc gttaccgatt acttaaccgg tttatataat aaacgtgagt
      301 ttgaaaaata cttatccact attaataaga gtcgctaccg tttccggaaa ttagggggaa
      361 tattctttga tttagataac tttaaacaaa tcaatgacca gtttggccat ttaatgggcg
      421 actttattct tcgagaaatc ggtaatatcc tgcgtcaaag ttttcgtcgt actgactttt
      481 tagcccgcat tggtggcgat gaatttgtag cgttttttga aatatcccat aaagaagata
      541 tcagggtttt tcaaaaccgc ttgttagcca atgttagcga actaaacaaa accaacaaat
      601 tgccctttat tgtttccatt agtatgggca ttgattgctt tgatgcgaaa agtccgtttt
      661 caatccatga attttttgct catcttgata aattgcttta cgaacaaaaa gaacaaaaac
      721 ccatctttca tcttcaccct gctgatgggg aatcttcgtt aaagcaaaaa tgaggcggtt
      781 aggcgtttac caaaaaactc ataaaacatt attttattag tttaaggatg aatgtgaaaa
      841 acaagtatta gtagacacaa aaaactgttt attaatactt ttattttgct tgggaaataa
      901 ttgcataaaa aacgctttaa tggtataata taaagcgtta tttatagtaa acaattaagg
      961 aggttaagat tgtggactac aaacaagctg ccaaagattt tctagaaaat gagaaacaat
     1021 tccatttagg agtaatcccg accgaacaat ccaatccgat tactcgtaat ttgagcccga
     1081 cgattgcgaa ggacacggct gccggaatca aaatgatttt gggggcggat gttaatattc
     1141 ccagtgctac agccaaagct ttcgcaacga aagaatttgc tcaattagtc gatgatatta
     1201 aacgcgttat tgatgagaaa aaaactttgt tgttttccag tgtgggggcc tcgggacgga
     1261 tggctttgca acttgatggc atgtggcgtt tattctggac atcaatgata acaaaaattc
     1321 ctgccaaacg gcaaatgttt ttagaaaaca tcgaacgatg caatagtttt atgacaggag
     1381 gggaccgcgc tttagttaaa acagccgaaa attatgaaga ttatatgact ttcggccgcg
     1441 aacaagttaa ggaagccaag gttggtcctg gtgatgtggt cgtcggttta gccgaatgcg
     1501 ggctttctgc cagcattaat ggttccgttt tggaagcgga tgaacgcggg tgtacgactt
     1561 attatattta ttgcaatcct aaagaaatct tagtgaaaca tcttgaccgt gttcgcgtcg
     1621 tttttgaacg cgaaaacatc attaaaatca gtttgttcgt cggtaatatg gccgttgctg
     1681 gttcaacgcg ga
//
LOCUS       DAOSGN010000215         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_73133_length_1690_cov_2.494801, whole genome shotgun sequence.
ACCESSION   DAOSGN010000215 DAOSGN010000000
VERSION     DAOSGN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73133_length_1690_cov_2.494801"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /gene="polC"
                     /locus_tag="PK087_03435"
     CDS             <1..581
                     /gene="polC"
                     /locus_tag="PK087_03435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOH18359.1"
                     /translation="QVPEWYIDSCKMIQYMFPKAHASAYVIMALRIGWFKVYRPLQYY
                     AAYFSRRANVFDIIAMAGGYSSINLRVKELEDLIKIKRASNKEIDIYNCLLLALEMTA
                     RGFTFQQIDIEKSDCRDFLIEGNSLIIPFRAMDNLGEATAKSITDARSEAMFSSKKDI
                     MRRTKINTTLFEKLSEIGAFKGLPNEDQIGLF"
     gene            603..1334
                     /locus_tag="PK087_03440"
     CDS             603..1334
                     /locus_tag="PK087_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOH18360.1"
                     /translation="MRFRSHNPVFQRVLKTAYEGENYEVASYRGVAFKTIYFIALTIL
                     GAIISLWAAMAHPNALGSLLPIALVVTIISSMLALWVPSLSQVFGSVYCLGEGLLVGF
                     VSLLFELEAPGVVLTAILATIVVLIVVTTLFVTRAIVVNSRFIRFLLIFSISMFLTQL
                     LLWIISFSFGITYSFGFSMLTSGIMVFIATLFLLFDLESITQVVEGRAPKQWEWYVSF
                     GLVFTIIWLYMEILPIIAQLFFDRN"
     gene            1423..>1690
                     /locus_tag="PK087_03445"
     CDS             1423..>1690
                     /locus_tag="PK087_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOH18361.1"
                     /translation="MDLIIYPQHLKGIVEAPPSKSYTHRAIICASLACGRSVIHNPLI
                     CNDTHATINALKAIGVNIVYYETRIEIDGLNEIHIPKKPIFCHES"
BASE COUNT          466 a          278 c          335 g          611 t
ORIGIN      
        1 atcaagttcc tgaatggtat attgattcat gtaaaatgat tcaatatatg tttcctaagg
       61 cacatgcttc ggcctatgtg attatggcat tacggattgg atggtttaaa gtatatcgac
      121 ctttgcaata ttatgccgct tatttttcta ggcgagctaa tgtttttgat atcattgcca
      181 tggctggcgg ttattcgtca attaatcttc gggttaagga actcgaagac ttaatcaaaa
      241 ttaagagagc ttccaataag gaaatcgata tttataactg tttgctttta gctttagaaa
      301 tgacagctcg aggctttact ttccaacaaa tcgatattga aaaatcggat tgtcgagatt
      361 tcttaattga aggcaatagt ttaattattc cttttcgagc catggataat ttaggtgaag
      421 cgaccgcgaa gtcaattacg gatgcacgga gcgaagcgat gttttcttca aaaaaagata
      481 ttatgcgccg aacaaaaatc aatacgaccc tttttgaaaa actttcagaa attggggcct
      541 ttaaaggtct tcccaacgaa gatcaaattg gattatttta aatatgtata aggaggtcaa
      601 ttatgcgttt tcgcagtcat aaccccgtct ttcaaagggt tctaaaaacc gcatatgaag
      661 gagaaaacta cgaagttgcc agttatcggg gggtagcttt taaaacgatt tattttatcg
      721 ctttgactat tttaggagca attatcagtt tatgggcagc gatggctcat cccaatgctt
      781 taggttctct tttaccgatt gctcttgttg ttactataat tagttcgatg ttagcccttt
      841 gggtgcctag tttatcccaa gtttttggtt ccgtttactg tttaggtgaa ggtttattag
      901 tgggatttgt gtccttatta tttgaacttg aagctccggg cgttgtgtta acggccatct
      961 tggcgacaat tgtagtgctg attgttgtga cgactttgtt tgtaacgcga gccattgtcg
     1021 tcaatagcag attcatccgt tttttactga ttttttcaat ttcgatgttt cttactcaat
     1081 tgttattgtg gattatttct tttagttttg ggattactta tagtttcggc ttttcaatgc
     1141 ttacttcagg aattatggtt ttcatagcaa cgctcttttt actctttgat ttagaaagta
     1201 ttacccaagt tgttgaagga agagccccga aacagtggga gtggtatgta tcttttggct
     1261 tagtgtttac catcatttgg ttatatatgg agatcttacc gattattgct caactctttt
     1321 ttgatcgtaa ttaataatca gggccaacac ttgtgttggt cttgtttttt ttgtttaaat
     1381 attcttgtga ttatagtata ataaaatgaa ggaaagttgt ttatggattt aataatttac
     1441 ccccaacact taaaaggaat tgtggaggca ccgccatcca aaagttatac gcaccgagct
     1501 atcatttgtg ctagtcttgc ttgcggtcgc tccgtaattc ataatccttt aatttgcaat
     1561 gatacacatg caactatcaa tgccttaaaa gccatcggtg tcaatattgt ttattatgaa
     1621 acaagaatcg aaattgatgg gttaaacgaa attcacattc ctaaaaaacc gattttctgt
     1681 catgaatcag
//
LOCUS       DAOSGN010000216         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_73606_length_1683_cov_2.209459, whole genome shotgun sequence.
ACCESSION   DAOSGN010000216 DAOSGN010000000
VERSION     DAOSGN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73606_length_1683_cov_2.209459"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1425
                     /locus_tag="PK087_03450"
     CDS             94..1425
                     /locus_tag="PK087_03450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH18362.1"
                     /translation="MFKEFQFPVFINEALLKLQFQKPTSIQERVIPLVLQGKNVIGKS
                     ETGTGKTHAFLLPLLSKVQSQKKVQICIVVPTRELAMQIYEETLKITGCANDFIDVRV
                     YVGGTDRDNEIERLNKSQPDVVIGTIGKIKDLAIQQNALQIHTAEAIVIDEADMVFAN
                     SEMEDMDNVFAKFSPQVQILAFSATFSEPILQFLHKYFGKLEVIDLSKKNISKANIVH
                     YFIPTKNKNKDELLKDLLTNMQPYLALIFANTKVKVDEIANYLADNNYRVGKLTGDLE
                     ARTRKQMLRRIKEGEFQFVVATDIAARGIDIAGVSHVINYELPNDLEYYVHRTGRTAR
                     ADFSGFAISFYDFDDDRYLDLLEQKGLKCRYRSLKNGELLPTKERNARSKRVIREAIY
                     EQKAHASTPLPKKIKPAYRKKRNEIIQKKIRKAKREFVSEIYRKRSQKQSE"
     gene            complement(1485..>1683)
                     /locus_tag="PK087_03455"
     CDS             complement(1485..>1683)
                     /locus_tag="PK087_03455"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOH18363.1"
                     /translation="ITVAIMGCAVNGPGEAMEADIGIAGGANSALLIKKGKILGRIDQ
                     SQIISTLKQEIIQTIELKKQG"
BASE COUNT          583 a          287 c          308 g          505 t
ORIGIN      
        1 agtttcccac aattttttca aaaaaacatg attttgtaaa agttcaaata acggaagcgg
       61 gttatcccgt ttcgcaagct attgaggtta aaaatgttta aagaattcca atttccagtt
      121 tttataaatg aagctttact taaacttcaa ttccaaaaac cgacatctat ccaagaacgc
      181 gttattcctc ttgttttaca agggaaaaat gtgattggta aaagtgaaac ggggacggga
      241 aaaacacatg cttttttgtt gcctttatta agtaaagtcc aaagccaaaa aaaagtgcaa
      301 atttgtattg ttgttccaac ccgcgagtta gcgatgcaaa tctatgaaga aacgcttaaa
      361 attactggct gtgcaaatga ttttatcgat gttagagtat atgttggtgg aactgaccgc
      421 gataacgaaa tcgagcgttt gaataaatca caacccgatg ttgttattgg aacaattgga
      481 aaaatcaaag atttagccat ccaacaaaat gcgcttcaaa ttcacactgc cgaagcgatt
      541 gtaattgatg aggctgatat ggtttttgca aactccgaaa tggaagacat ggataatgtg
      601 tttgcaaaat tctcgccgca ggtacaaata ttagcttttt cagccacttt ttccgaaccg
      661 attttacaat ttttgcataa atattttgga aaactggaag ttattgattt atcaaagaaa
      721 aacattagca aggcgaatat tgtacattat tttattccaa ctaaaaataa aaacaaagat
      781 gagttgttaa aggacttatt aacaaatatg caaccttatt tagcactcat cttcgccaat
      841 actaaagtaa aagttgatga aatcgctaac tatttagccg ataataatta tcgggtaggg
      901 aaattaactg gggatttaga agcaagaaca cgaaaacaaa tgcttagacg gataaaagag
      961 ggggagtttc aatttgttgt tgctaccgat attgctgctc gaggaattga cattgctggg
     1021 gttagtcatg ttatcaatta tgaattaccc aacgacttag aatattatgt ccatcggaca
     1081 ggacgaactg ctcgtgctga tttttccggt ttcgccattt ccttttatga ctttgatgat
     1141 gatcgctatt tagatttact tgaacaaaag ggcttaaaat gtcgttatcg aagcttaaag
     1201 aatggtgaat tattaccgac caaagaacga aatgcccgct ctaaacgggt aattcgtgaa
     1261 gctatctatg agcaaaaagc ccatgcaagc actccgcttc caaaaaaaat taaaccagct
     1321 tatcgcaaaa aacgtaatga aatcatccaa aagaaaattc ggaaagcaaa acgggaattt
     1381 gtaagtgaaa tatatcggaa gcgttcacag aaacaaagtg aataatcgac taaggatttt
     1441 aatttcaggt taaattgact tatcaaattt ttaaagcaag gccattaacc ttgctttttt
     1501 agttcaattg tctgaataat ttcttgtttt aaagttgata taatttggga ttgatcaata
     1561 cgaccaagga ttttaccttt tttaattaac aaagcactat ttgctccacc agctatcccg
     1621 atatcagctt ccatagcttc gcccggaccg ttgacggcac aacccatgat agcaactgta
     1681 atg
//
LOCUS       DAOSGN010000217         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_74000_length_1677_cov_3.257090, whole genome shotgun sequence.
ACCESSION   DAOSGN010000217 DAOSGN010000000
VERSION     DAOSGN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74000_length_1677_cov_3.257090"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK087_03460"
     CDS             complement(<1..532)
                     /locus_tag="PK087_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOH18364.1"
                     /translation="MEQIKQFLIDEATKVITTAILIGGTIIVFIIIGLIVGRFIRRHK
                     QKRRRAVTLAKMIQSIFRYTVSIIIIIIILDVWDVNIMPVLAGAGILGLAIGFGAQSL
                     IKDLIAGMAIVFENYYDIDDVIEINGFKGRVEELGLKSTRIVNWKGEVKTFFNGDIKD
                     VVNYSKNPSTGIVDIEV"
     gene            complement(535..1311)
                     /locus_tag="PK087_03465"
     CDS             complement(535..1311)
                     /locus_tag="PK087_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH18365.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLGAALAREKQKILLVDLDEQAN
                     ATIGIGLAREHCQVTLYEVMIGKQSIKDAIYETQEPDIDIIPASNRLSTLDTDLINVE
                     NKVWLLAEKLQLIREAYDFILLDCPPSFGVIIDNALFASDSVIIPVECEYFAYDALTQ
                     MVNKINQVQTHKNPLKKKLTIEGVLLTKLDNRNIFGYKIIDQVQNLFPTKTFKTIINR
                     SSHLQEAPMLGKSVLKTAYHSRASKEYRALALEIIQNKAR"
     gene            complement(1323..>1677)
                     /locus_tag="PK087_03470"
     CDS             complement(1323..>1677)
                     /locus_tag="PK087_03470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH18366.1"
                     /translation="KNVQLINKRVEELGVEDRNFFDITVARAVAPLAILLELIIPFTK
                     TKGFFLAMKSRQYEQELQASQTALLKLRSEVADVFAYELSENMGRRVIIKVLKKAITA
                     AMYPRRFVQIKQKPL"
BASE COUNT          474 a          363 c          308 g          532 t
ORIGIN      
        1 caacttcaat atccacaatc ccggtggaag gatttttaga atagttgaca acatccttaa
       61 tatctccgtt gaagaaggtt tttacttccc ccttccagtt aacgattctc gtggatttta
      121 agcctaattc ttctacccgc cctttaaaac cgttgatttc aatgacatcg tcaatatcat
      181 aataattttc aaaaacaatg gccatgccgg caattaagtc cttaattaaa ctttgagcac
      241 caaaaccgat ggccaaaccc aaaattcccg ctccggctaa caccggcata atattaacat
      301 cccatacatc aagaatgatg ataatgataa tgatgctgac ggtgtaacgg aaaatgcttt
      361 gaatcatctt tgcaagcgtc accgcccgtc ggcgtttttg tttatgacgg cgaataaagc
      421 gtccgacaat taagccgata ataataaaga caataatcgt tcccccgatt aaaatcgcgg
      481 tggtaattac tttcgttgct tcatctatta aaaattgttt gatttgttcc atatttacct
      541 cgctttgttt tggattattt ctaaggctaa agcccgatac tctttactgg cacgggaatg
      601 gtaagccgtt tttaaaacac ttttcccaag catcggtgct tcttgaagat ggctggaacg
      661 attaataatc gttttaaagg ttttagtggg aaataagttt tggacctggt caataatctt
      721 atagccgaag atattccgat tatccagttt cgttaataaa accccttcaa tggttaactt
      781 tttctttaaa gggtttttgt gcgtttgcac ttggttgatt ttgttaacca tttgtgttaa
      841 ggcatcgtag gcgaagtatt cacattcgac gggaataatg acgctgtcgc tggcaaataa
      901 ggcattatca ataatgacac caaatgaagg cggacaatcc aaaagaataa aatcataggc
      961 ctcgcgaatt aactgcagtt tttcagctaa caaccacacc ttattctcca cattaatcaa
     1021 atccgtatcc aaggttgaca agcgattgga agcgggaata atatctatat ccggttcttg
     1081 ggtttcataa atcgcatcct taatgctttg tttcccaatc atcacttcat aaagcgttac
     1141 ttgacaatgt tcacgcgcta aaccgatgcc aatggtagca ttggcctgtt catcaagatc
     1201 aactaataaa atcttttgtt tttcgcgagc cagggctgct cctaaattga tggcggttgt
     1261 cgttttccca accccgcctt tttgattggc gatggcaatg atttttccca tgtcacacct
     1321 cgttataatg gtttttgttt gatttgtaca aagcgacgcg gatacattgc tgccgttatc
     1381 gcttttttta ggactttgat aatgacgcgc ctgcccatgt tttcagaaag ttcgtaagcg
     1441 aaaacatcag ccacttcact tcgtagtttt aataaggcgg tttgcgaagc ttgcagttct
     1501 tgttcatatt gtctgctttt catcgccaag aaaaaaccct tcgttttcgt aaacggaatg
     1561 atgagttcta acaagattgc taagggcgcc actgccctag caacagtaat atcgaagaag
     1621 ttacggtcct caacacccag ctcctcaacc cgcttattaa ttaattgcac atttttt
//
LOCUS       DAOSGN010000218         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_74057_length_1676_cov_3.851943, whole genome shotgun sequence.
ACCESSION   DAOSGN010000218 DAOSGN010000000
VERSION     DAOSGN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74057_length_1676_cov_3.851943"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..473)
                     /locus_tag="PK087_03475"
     CDS             complement(54..473)
                     /locus_tag="PK087_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18367.1"
                     /translation="MNEERMKILEMLAEGLISAAEANELLSTLDKTKVEINGKEVKEV
                     QVLKKSSSKFLYVKALSSEGDRVNLKLPLALVKATLKSGNGENFLGKMKINGMDNEFV
                     KDAIDYELIMQYIENDMDGNIVDVESAAGDSVNIYIE"
     gene            complement(521..901)
                     /locus_tag="PK087_03480"
     CDS             complement(521..901)
                     /locus_tag="PK087_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOH18368.1"
                     /translation="MKKEVIAKCPICGEELTITRLHCHNCGVEINGEFALNKLCRLSK
                     EQLTFVLLFLKNQGNIKAIEKDMNISYPTVKKLLNDVLSNLGFDVSDEATSKTALQRQ
                     EILDKLAKKEISFDEANQQLKNLK"
     gene            complement(910..1239)
                     /locus_tag="PK087_03485"
     CDS             complement(910..1239)
                     /locus_tag="PK087_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18369.1"
                     /translation="MKKRILKKIAQGNLTPYAAYELLYQKKLRRARFIKIHMSVSEHP
                     WLSAFINTLFFLAFPIAWGEKILMRMINKKGLNIDYLTFKNLLDYSSGTEIDVLTEEA
                     KVQIFIF"
     gene            complement(1387..1614)
                     /gene="acpP"
                     /locus_tag="PK087_03490"
     CDS             complement(1387..1614)
                     /gene="acpP"
                     /locus_tag="PK087_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004802993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH18370.1"
                     /translation="MIFEKVRKLIAKELNLKEENIKLESKITEDLGADSLDAVELIMA
                     IEDEFSVQVSDEAAQNIRTVGDIVNLLEKNK"
BASE COUNT          527 a          314 c          206 g          629 t
ORIGIN      
        1 agtaaaataa caaaatgata gcaaacatcc tcatgtttcc taccattttg ttattactca
       61 atataaatat tgacggaatc accggcagca gattcaacat caacgatatt cccatccata
      121 tcattttcga tatattgcat aatcaattca tagtcaatgg catctttaac aaattcatta
      181 tccataccat taattttcat tttacccaaa aaattctcac catttcctga tttcaaagta
      241 gctttaacta aagcaagcgg cagtttaagg ttgactctgt ccccctctga cgatagtgct
      301 tttacataaa gaaatttgga agagcttttt ttaagaactt gaacttcttt gacttcttta
      361 ccattaattt ccactttcgt tttatccaag gtcgataaca attcattagc ttccgctgcg
      421 gatataagcc cttcggctaa catttctaat attttcattc tttcttcatt catatttttt
      481 ctcctttttt tacttagttt taactatctt ttcatcgttc ttacttcaaa tttttcagtt
      541 gttgattagc ttcgtcaaaa gaaatttcct ttttcgcaag tttgtcgaga atttcttgtc
      601 tttgtaaagc ggtcttacta gttgcctcgt cgctaacatc aaagcccaag tttgataaaa
      661 catcattcaa taactttttc acggttggat atgatatgtt catgtctttt tcaatagctt
      721 tgatattacc ttgattcttt aaaaacaata gaacaaaggt taattgttct ttcgataaac
      781 ggcataattt gtttaaagca aactcgccat taatttcaac tccgcaatta tggcaatgca
      841 aacgggtaat tgttaattct tcaccacaaa tggggcattt cgcaataact tcttttttca
      901 taactcctcc taaaaaatga atatttggac tttcgcttct tcagttagaa catcaatttc
      961 agttccgctg ctataatcta gtaggttttt gaaagttaga taatcaatgt taagtccttt
     1021 tttattaatc atccgcatta agattttttc accccaagca ataggaaaag ctaaaaaaaa
     1081 caaagtatta atgaaggcac tgagccaagg gtgttcactc acactcatat gaatttttat
     1141 aaaacgcgct cgtcgaagtt tcttttgata aagtagttcg taagcagcat agggagttaa
     1201 gttcccttgg gcaatttttt taagaattct tttcttcacc atttcacccc ttgataatat
     1261 catataataa ccaatttatt ttgtcaagct aaaaataaat atttttaatt ttggagttga
     1321 ttttattaat ttatacttaa attattttgc atagaaaaag gccttaatga atctaaggcc
     1381 taagtgttat ttattttttt ctaataaatt gacgatatcg ccaacagtgc gaatgttttg
     1441 agcagcttca tcactaactt gaacactaaa ttcatcttca atcgccataa ttaattcaac
     1501 ggcatcaagt gaatcagctc ctaaatcttc cgtaatttta gattccaatt taatattttc
     1561 ctcttttaaa tttaattctt tcgcaatcaa ttttcttact ttttcaaaaa tcataataaa
     1621 cctcctcgat ttagttacta ttatatttaa aaaaacgaaa aatgtcaacc aatatc
//
LOCUS       DAOSGN010000219         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_74325_length_1673_cov_2.514215, whole genome shotgun sequence.
ACCESSION   DAOSGN010000219 DAOSGN010000000
VERSION     DAOSGN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74325_length_1673_cov_2.514215"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK087_03495"
     CDS             complement(<1..489)
                     /locus_tag="PK087_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOH18371.1"
                     /translation="MNNKSKTTFKKILDYFVKTLNGMAYGLFSTLIIGTIIGTIGTLV
                     NVPAIIHLADLLKTMTGIGIGVGIAWSLQLEGLKLIAAGVAGGIASTLSFGRPGDPFV
                     EYLCAIGAIEVVRLVLRKKTPVDIIIVPLFSAAVAYGLFLLINQPISSMMRAIGNFIQ
                     WAT"
     gene            complement(559..1350)
                     /locus_tag="PK087_03500"
     CDS             complement(559..1350)
                     /locus_tag="PK087_03500"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOH18372.1"
                     /translation="MRKQRIWELDALRGWAFVLMVFDHLTYDLGRIFLNIWFDGPSNH
                     WLYRLCKACADYRDLDIALLLRVLLVSGVFLFVSGISANLSRSNFRRGLKLLFIALAL
                     TGITSLLEIAFGFVGATVYFGILHCLAIAMLLTPLFLKMNPYVLLGLGAVVIGVGALM
                     ELGLLSFSSPLVVFLNRYHLLVPFNIYAYNFSSGDYYPLIPYMGIYMVGIVVGKYLYS
                     QKKSLFKKDYNLKPLNYLGRNALWLYFVHQIVLFGFLYVIGWIFV"
     gene            complement(1448..>1673)
                     /locus_tag="PK087_03505"
     CDS             complement(1448..>1673)
                     /locus_tag="PK087_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HOH18373.1"
                     /translation="IAIMIDKEVSLFDPKSLAIIASILIIGLGGSYNYAGGMIPIFGM
                     KAPAIASAAIVGILLNLIFVLMDKLVHAKK"
BASE COUNT          608 a          359 c          278 g          428 t
ORIGIN      
        1 ggtcgcccat tggatgaaat taccaatggc ccgcatcatt gaactgatgg gctgattaat
       61 caataaaaac aagccataag caaccgccgc cgaaaaaagc ggaacaatga tgatatcaac
      121 cggcgtcttc ttccttaaca ctaagcgaac tacttcaatc gcaccaatag cacataagta
      181 ctccacaaag ggatcacccg gccgaccaaa gctcaatgtt gacgcaatgc cacctgcgac
      241 accggccgca attaatttca agccctctaa ttgtaaactc caagcgatgc ccacaccgat
      301 accgattccc gtcatcgttt ttaaaagatc ggctaggtga attatcgcag gaacattaac
      361 taaagtgcca atcgtgccaa taatcgtccc aatgattaaa gttgaaaaca gaccataagc
      421 catgccattt agggtcttta caaaatagtc taagattttt ttaaaagtcg ttttgctttt
      481 attattcatt tttatttcct caattttgtg ttaccattct accataaatt ctcccaaaac
      541 acaaaaattt tggtttcatt aaacaaatat ccagccgata acatataaaa agccgaataa
      601 gacaatttga tggacaaagt aaagccataa agcgtttctt cccaaataat taaggggctt
      661 aagattataa tcttttttaa ataagctttt cttttgggaa tataaatatt tacccaccac
      721 aattccgacc atataaattc ccatataggg aattaaagga tagtaatctc cggaactaaa
      781 attataagca taaatattaa aaggtactaa taaatgatag cggtttaaaa aaacgaccaa
      841 aggggaagaa aacgatagaa gccccaattc cattaaagcc ccaacaccga tgacaaccgc
      901 gcctagtccc aataaaacat aaggattcat ttttaaaaat aacggtgtta gcaacatggc
      961 gattgctaaa caatgaagga tgccaaaata aacagttgcg ccaacaaaac caaaagctat
     1021 ttctagtagt gatgttattc cggttaatgc taaagcaatg aagaggagtt ttaaaccacg
     1081 acgaaaatta ctacgcgaaa ggttggcgga tattcccgaa acaaataaga acactccgga
     1141 tactagcaaa acccgcaaca agagtgcgat atccaaatca cgataatcgg cacatgcttt
     1201 gcataaacgg taaagccaat gattactcgg tccgtcaaac caaatattaa gaaagatacg
     1261 accaagatca taggtcaaat ggtcgaaaac cattaataca aaagcccaac cccgtaaagc
     1321 atcaagttcc catatccgtt gttttctcat ttgtagctcc tcttttgtta gctaagttat
     1381 tataacatga atgattgatg atttaaatag tcaaaaaaag ccggaaggca tttccggctt
     1441 gtgaagatta ttttttagcg tggactaatt tatccatcaa cacgaagata agatttaaaa
     1501 gaatgcccac aatcgctgcc gaagcaatgg ctggagcttt catcccaaaa ataggaatca
     1561 ttccgcccgc ataattgtac gatccaccaa gcccgatgat taaaatggaa gcaataatcg
     1621 ctaaactctt aggatcgaat aaactcactt ctttgtcaat cataatggca atg
//
LOCUS       DAOSGN010000220         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_74490_length_1670_cov_3.164706, whole genome shotgun sequence.
ACCESSION   DAOSGN010000220 DAOSGN010000000
VERSION     DAOSGN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74490_length_1670_cov_3.164706"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..>1670)
                     /locus_tag="PK087_03510"
     CDS             complement(95..>1670)
                     /locus_tag="PK087_03510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOH18374.1"
                     /translation="DSINKVRNTLQTMQALVGARNAIDTTDKFYEDEQNFFDENSPFI
                     AGFEHQLALALHTSPFKQELIKKYGKQLFALIETNLKTFKPEIIEDLQEENKLVTEYT
                     KLTASAKIEFDGKINNLSQMAPYAIAPNRETRKQAETAVMKFFEANEAKYDDIYDKLV
                     KVRTRIAKKLGYENFVEVGYARLGRTDYDSKMVANYRKQVEKDLVPVAVSLFKEKAKR
                     LGIKDLKSYDLGINFASGNPKPQGDRDFLVSQAQKMYHAMSQETGEFIDFMIEHELLD
                     LEAKQGKAGGGFCTYFPDYQSPFIFSNFNGTAGDVDVLTHEAGHAFQVFSSRHFAVPE
                     YYWPTMESAEIHSMSMEFFAWPWIDLFFGKDSDKYKYEHLAGTITFIPYGVAVDEYQH
                     EIYEHPELTPDERKAVWRRIEKKYLPYKVYENPFLEKGTYWFRQGHIFSTAFYYIDYT
                     LAQVCAQQFWILNQKDHEQAWKQYYHLCTLGGSKSFLGLIEAAGLKNPFVDGTVAMIA
                     KELQKFLDNFDQTKLQ"
BASE COUNT          464 a          376 c          311 g          519 t
ORIGIN      
        1 gccggccgct aaggcttcat ggcgtaagtt aaacaaagcc gaaaattgct tagtattctt
       61 taaaaaggtg attcttgctg ctagttgatt catattattg taatttcgtt tgatcgaaat
      121 tatctaaaaa cttctgtaat tccttcgcaa tcatggcaac ggtaccatcc acaaacggat
      181 tctttaaacc ggccgcctca attaaaccca agaaagattt acttccgccc aaagtgcata
      241 aatgatagta ttgtttccaa gcttgctcat gatccttttg gtttaaaatc caaaactgct
      301 gagcacagac ttgggctaag gtataatcga tataatagaa agccgtactg aaaatatgtc
      361 cttggcggaa ccaataagtc cctttttcta agaagggatt ctcatagact ttatacggca
      421 aatatttctt ttcaatccgt cgccaaacgg cttttctttc atccggggtt aattccggat
      481 gttcgtagat ctcatgctgg tattcatcaa cggccacacc ataaggaata aaggtaatag
      541 tcccggctaa atgttcatat ttatatttat cgctatcttt tccaaagaat aaatcaatcc
      601 acggccaagc gaaaaactcc atactcatgg aatggatttc cgccgattcc atggtcggcc
      661 aataatattc cggaacggca aaatgacggc tggaaaaaac ctgaaaagca tggccggctt
      721 catgggtcaa aacatcgaca tctccggccg tcccgttaaa gtttgagaaa ataaacggtg
      781 attggtaatc cggaaaatat gtacaaaagc cgcccccggc tttgccttgc ttagcctcta
      841 agtccaataa ctcatgctca atcatgaaat cgatgaattc accggtttct tgactcatgg
      901 catgatacat cttttgggct tggctaacta agaaatcacg gtcaccttgc ggtttgggat
      961 tccctgaagc aaagttaatc cctaagtcat aactctttaa atctttaatg cccaagcgtt
     1021 tcgccttttc cttaaaaagg gaaaccgcta ccggcaccaa gtctttttcc acttgcttgc
     1081 ggtaattggc caccatcttg ctgtcgtaat cggtgcggcc taaacgggcg tatccgacct
     1141 ccacaaaatt ctcatagccc agttttttcg caatcctcgt tctaactttt actagtttat
     1201 catagatgtc atcgtacttc gcttcattgg cttcaaaaaa cttcatgacc gccgtttcgg
     1261 cttgttttcg cgtttcccga ttaggagcaa tcgcatatgg ggccatctgc gataaattgt
     1321 tgattttgcc atcaaattca attttagcgc tggctgttaa tttagtatat tccgtcacca
     1381 acttattttc ttcctgcaaa tcttcaatga tttccggttt aaaggttttt aagttggttt
     1441 cgattagggc aaatagttgc ttcccatact ttttaattag ttcctgttta aaggggctgg
     1501 tatgcaaagc gagggccaat tgatgttcaa accccgcaat aaacggggaa ttttcatcaa
     1561 agaaattttg ttcgtcttca taaaacttat ccgttgtatc aatggcattg cgtgccccca
     1621 ctaaggcttg catcgtttgt aaagtattac gcactttatt aatactatca
//
LOCUS       DAOSGN010000221         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_74956_length_1664_cov_2.551274, whole genome shotgun sequence.
ACCESSION   DAOSGN010000221 DAOSGN010000000
VERSION     DAOSGN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74956_length_1664_cov_2.551274"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..>1664)
                     /locus_tag="PK087_03515"
     CDS             complement(57..>1664)
                     /locus_tag="PK087_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH18375.1"
                     /translation="EQHMMIKNKLTLLLLVFVLLVISGCGPKKDPLREAFPRNDIYYE
                     LFVRSFADSDGDGIGDFQGLIAKLDYLESLGVTGLWLMPIHPSPSYHGYDVIDYYDVN
                     PDYGTLDDFKQLLKETKKRGIKIMLDMVFNHSSDQHPWFKAALANDAQYRDYYTFATP
                     TTNRSLLGSWGQNIWHHQDDQYYVGYFSPSMPDLNFFNKEVHKEIAAISQFWLDLGVS
                     GFRLDAAAHFYGINEYQNQRYDDYENIIFLQVLRNNLRKLNSQFYLTGEIYITAESIV
                     APYFRGLDSPLDFPVALKTSTIAGANGSTTYVNSLIKIYELYAKENPAFISAPFLFNH
                     DMDRVADQLGGDMEKMKLAAEMLLVLPGSPILYYGEEVGMHGVKANGELSQGIAVWDE
                     TRRLPFPFGDEYETTWFTDSNFPSVLANQKLKPLSLQITEPTSLFKTYQQMIAVRKAN
                     PALYYGNHLSPYEKNNYQLQGFYREFSYLNHYQKVLVIHNLSRQAQAIEALGTILYLS
                     NTTDFTDVCEIPPKSTIIFDVSEADNA"
BASE COUNT          524 a          330 c          326 g          484 t
ORIGIN      
        1 aaggtttttt tctttgttta aaaagattgg ataaagaaaa actatgccaa aagattttaa
       61 gcattatccg cctccgagac atcaaaaatg atggttgatt tgggaggtat ttcacaaaca
      121 tccgtaaaat ccgttgtatt tgataaatag agaatcgtcc ctaaggcttc aatcgcttgc
      181 gcttggcgag aaagattatg aatgactaaa actttttggt aatgatttaa ataactaaat
      241 tctcggtaaa aaccttggag ctgatagttg ttcttttcat agggcgaaag atgattccca
      301 taatataaag ccgggttggc tttacgaacg gcgatcattt gttggtaggt tttgaataaa
      361 gaagtcggtt cagtgatttg taaggacagg ggcttcagtt tttgatttgc taaaacgctg
      421 ggaaagttcg aatccgtaaa ccaagtagtt tcatattcat caccaaaggg gaagggcaag
      481 cgccttgttt catcccaaac ggcgatgcct tgggacaatt cgccattggc tttaacacca
      541 tgcataccga cttcttcacc ataataaaga attggtgaac ccggaagcac caatagcatt
      601 tcggccgcta acttcatttt ttccatatca ccaccaagtt ggtcagccac ccgatccata
      661 tcatggttaa acaaaaaggg ggcactgata aaggccggat tttctttcgc atataattca
      721 tatatcttaa ttaaactatt aacataggtc gtacttccgt tggctccggc aatcgtcgat
      781 gttttaagag ccaccggaaa atctaaaggc gaatctaaac cgcggaaata aggtgcgaca
      841 attgattcag ccgtaatata aatttctccg gttaaataga attgagaatt taattttcgt
      901 aaattatttc gtaacacttg taagaagatg atattttcat aatcatcata gcgttggttt
      961 tggtattcat taatgccata aaaatgagcg gcggcatcca gccgaaaacc actaacacct
     1021 aaatcaagcc aaaattggct gatggcggca atctccttat ggacttcctt attaaaaaaa
     1081 tttaaatccg gcatcgaagg cgaaaaataa ccgacatagt attggtcatc ttggtgatgc
     1141 caaatgtttt gcccccagct gcccaataaa ctgcgatttg ttgtgggcgt tgcgaaagta
     1201 taataatccc ggtattgggc atcattcgct aaagccgctt taaaccacgg atgttgatcg
     1261 gaactgtggt taaaaaccat gtccagcata attttaatgc cccgtttttt tgtttctttt
     1321 aaaagctgtt taaaatcatc taaagtgccg taatcgggat taacatcata atagtcaatg
     1381 acatcataac cgtgataact gggactgggg tgaatgggca ttaaccataa gcccgtcact
     1441 cccaagcttt ccaaataatc cagcttcgca ataaggcctt gaaaatcacc aatgccatca
     1501 ccatcgctat ccgcaaaact ccgaacaaag agttcgtaat aaatatcatt ccgcggaaaa
     1561 gcttctctta aggggtcctt cttgggaccg caaccactga ttactaggag cacgaaaact
     1621 aataaaagta aagtaagttt gtttttaatc atcatatgtt gttc
//
LOCUS       DAOSGN010000222         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_75098_length_1662_cov_2.818917, whole genome shotgun sequence.
ACCESSION   DAOSGN010000222 DAOSGN010000000
VERSION     DAOSGN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75098_length_1662_cov_2.818917"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="PK087_03520"
     CDS             complement(<1..1579)
                     /locus_tag="PK087_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH18376.1"
                     /translation="MGKSKVLTNEPLIKIVGMKKYFPLKKTRLFQREILTVKANDGVD
                     LVIHKGETLGLVGESGCGKSTLGRVLLQLYPLTSGKIIYYGYTLEDYSPRYLFKEINS
                     LKASRLKYEKYSKQIQAIEAKMASQKNNPAFQEQVKNNEQEIAKLKAECNQIASDCRS
                     QFGIFFASNNMRAIKQAIKMDALIYKNKEKVAQLELKQVKLEQDLEKAKKIKNVKAEA
                     DVAALKKQLENADLNLIADLNKKLSIAERKAQHHVIAVDIDIRETQKKINLINDDTQR
                     FIARIKSLLEKPIELNTEIVAKLVERKQQHFVREKRIKQLQDFNLFTKEKAMISRLNK
                     KIEKLINKAANVTGAFILSDDIAGISDTLFKNIDALHTIQMNKEEITLLEVKKFKIEQ
                     KIADDKGTYTDFQKDVFQERIEKINQKLANLHAENEQLTEKTKQLTQEIKEANAKLSD
                     HPMYEELEAKKEKGLDLSRLTGEEMRRLRQDMQLIFQDPYSSLNPRLTVGQIIGEGLK
                     AHDVESKTKDNYQDMILK"
BASE COUNT          444 a          309 c          267 g          642 t
ORIGIN      
        1 cttttaaaat catatcttgg taattgtctt tcgtttttga ttcgacatca tgagctttca
       61 aaccttcgcc aataatttgg ccgacagtta agcgcggatt taacgatgaa taaggatctt
      121 ggaagattaa ttgcatatcc tgacgtaaac gacgcatttc ttcgccggtc aagcgggata
      181 aatccaagcc tttttctttc ttagcttcca attcttcata catcggatgg tcagacaatt
      241 tcgcattagc ttctttgatt tcttgtgtca attgcttggt tttttccgta agttgttcgt
      301 tttcagcatg taaattagca agtttttgat ttattttttc aatccgttct tggaaaacat
      361 ctttttgaaa atcagtataa gttcctttat catcagcaat tttttgctcg attttaaact
      421 tcttaacttc caataaggta atttcttctt tgttcatttg aatcgtgtgc aaggcgtcaa
      481 tattcttaaa caaagtatcg gaaatgccgg caatatcatc ggataaaatg aatgctccgg
      541 taacattggc agctttatta attaattttt caatcttttt attaaggcga ctaatcatcg
      601 ctttttcttt tgtgaagaga ttaaaatctt ggagttgctt aattcttttt tcacgaacaa
      661 agtgctgttg tttgcgctca accagtttag caacaatttc cgtatttaac tcaattggtt
      721 tttctaacaa ggatttgatc ctcgcaatga acctctgggt atcatcatta attaaattga
      781 ttttcttttg cgtttcgcga atatcaatat caacggcgat aacatggtgt tgcgcttttc
      841 tttccgcaat cgacaacttt ttattcaagt cggcaatcaa gtttaaatcg gcattttcta
      901 attgcttttt aagcgctgca acatctgctt ccgccttcac atttttaatc tttttagctt
      961 tttctaagtc ctgttcaagt ttaacttgtt ttaattctag ttgagcaacc ttttctttat
     1021 ttttataaat caaggcatcc attttaatgg cttgtttaat cgctctcata ttattagaag
     1081 caaagaagat tccaaattgg ctacgacaat cactcgcaat ttgattacat tctgctttta
     1141 acttggctat ttcttgttca ttattcttta cttgctcttg aaaagcagga ttgttttttt
     1201 gactagccat tttggcttca attgcttgaa tctgcttaga atatttctcg tatttaaggc
     1261 gagatgcttt taaactgtta atttctttaa ataaatagcg tggtgaatag tcttccaaag
     1321 tataaccata ataaatgatt tttccggaag taagcgggta aagctgtaat aaaacacggc
     1381 ctaaagttga ttttccacaa ccgctttcgc caactaagcc caaagtttcg cctttatgaa
     1441 tgactaaatc gacgccatca ttggctttaa ccgttaagat ttcgcgttgg aataaacggg
     1501 tttttttcag tggaaaatat tttttcatgc ccacaatttt aattagtggt tcgtttgtta
     1561 atactttact ttttcccatg ttgtctctcc ttgacattcg gatagaaaca gcgtacccct
     1621 tgaacggcgg atactttaat taaatccggc acttcattga ta
//
LOCUS       DAOSGN010000223         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_75101_length_1662_cov_2.732421, whole genome shotgun sequence.
ACCESSION   DAOSGN010000223 DAOSGN010000000
VERSION     DAOSGN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75101_length_1662_cov_2.732421"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PK087_03525"
     CDS             complement(<1..762)
                     /locus_tag="PK087_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOH18377.1"
                     /translation="MKKPCPYGTHRVIAPVGVLPQAADQLDNTMVCQDNEILIDVHTL
                     NIDSASFTQLKQATDSNPEAMKKMILEIVEKRGKMQNPVTGSGGMLLGVIKQIGKNLK
                     RDLQLGDKIVTLVSLSLTPLKIKKIKEVHLEKDQVDIEGEAILFESGIYAKIPNDLSE
                     HLVLSALDVAGAPAQVAKLVKPNDTVLVLGAAGKSGVLCCYQARKNAGPKGKVIALIS
                     NTRKLADLQSLNVCDEIILADARDPMAVYNEVMRVN"
     gene            complement(759..1586)
                     /locus_tag="PK087_03530"
     CDS             complement(759..1586)
                     /locus_tag="PK087_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOH18378.1"
                     /translation="MEKLIITCALSGAEVTKEHNPSVPYTALEMAESAHQAYLAGASI
                     IHLHARWDDGTPTQDAGRFWEIMEAIKAKCPEVIIQVSTGGAVGMSRDERLGPVTLNP
                     EMATLDCGTLNFGGDEIFVNTENDIKYFAQALESRGIYKELECFEKGHVDTVLRLAKR
                     GFINPPYHFSFVLGVNGGMTGEERDFLFLKDSIPAEATFSVAGIGRFEFPLAELAIIH
                     GGHVRVGLEDNIYLEKGVLTPSNAALVAKVVELAKKHHREIATPDDARRILKMEKKK"
BASE COUNT          465 a          410 c          301 g          486 t
ORIGIN      
        1 gttaacacgc atgacttcat tataaaccgc catcggatca cgagcatcgg ctaaaataat
       61 ttcatcacaa acattcaaag attgtaaatc ggcaagttta cgggtattgc tgattaaagc
      121 aatgactttc cccttcggtc cagcattttt gcgcgcttga taacagcata aaacaccgct
      181 ttttccggct gcgccaagca ctaacaccgt atcattaggt ttaaccaatt tcgctacttg
      241 agccggagcc ccggcgacat ctaaagccga taatactaaa tgttcactta aatcattggg
      301 aattttcgca taaattccgc tttcaaacaa aatggcttca ccctcaatat ccacttgatc
      361 tttctccaaa tggacttctt taattttctt aatctttaaa ggtgttaacg ataaggaaac
      421 taaagtaacg attttatcgc caagctgtaa atcgcgttta aggttcttac cgatttgttt
      481 gattacacct aataacatcc cgccgctacc ggtaaccggg ttttgcattt taccccgttt
      541 ttctacaatt tccaaaatca tctttttcat cgcttccgga ttactatcag tggcttgttt
      601 tagttgtgtg aaggaagcgg aatcaatatt caaggtatga acatcaatta agatttcatt
      661 atcctgacaa accatcgtat tatcgagttg atcggcagct tgtggaagaa cgccaaccgg
      721 agcaattacc cggtgcgttc catatggaca gggttttttc attttttctt ctccattttt
      781 aatattcttc tggcatcgtc cggggtagca atttcccgat gatgtttttt cgccaattcc
      841 acaaccttag caactaaagc ggcattggaa ggggtcagga cacctttttc aagatagata
      901 ttatcttcta ggccgactct gacatgtccg ccatgaataa tcgctaattc cgcaagcgga
      961 aattcaaagc ggccgatgcc ggcaacactg aatgttgctt cggcaggaat gctgtctttc
     1021 aaaaacagaa aatcacgttc ttcacccgtc ataccgccat tgacacctaa aacaaaacta
     1081 aagtgatagg gcggattaat aaatcctctt ttcgccaggc gtaaaaccgt atccacatgg
     1141 cctttttcaa aacattccaa ttccttgtaa ataccacgac tttctaaggc ctgagcaaaa
     1201 tatttgatat cattttcggt attcacaaat atttcatcgc ccccgaaatt taaagtcccg
     1261 caatccaaag tcgccatttc cggatttaaa gtcaccgggc ccaagcgttc gtctctactc
     1321 atgccaactg cgccaccggt ggatacttga atgatgactt ccgggcattt agctttaatc
     1381 gcttccatga tttcccaaaa acgaccggca tcctgcgtcg gcgtcccatc atcccaacga
     1441 gcatgaagat gaataattga tgctcccgct aaataagctt gatgagcgct ttcagccatt
     1501 tccaaggccg tatagggaac actcggatta tgttctttcg tgacctcagc tcccgaaaga
     1561 gcacaagtaa taattaattt ttccatggtc taccgccttt gcaaagcctt gggaaccaca
     1621 caggttccac ttgctttcgc aaccaaaagg ggcgtttcta aa
//
LOCUS       DAOSGN010000224         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_75125_length_1662_cov_1.878034, whole genome shotgun sequence.
ACCESSION   DAOSGN010000224 DAOSGN010000000
VERSION     DAOSGN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75125_length_1662_cov_1.878034"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PK087_03535"
     CDS             <1..422
                     /locus_tag="PK087_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH18379.1"
                     /translation="SVVNGQPWFVHAVSQAETMKKVITACTGLGMNRFIHQAQAFFVV
                     TETRDKLISTTISKIKKLDFQTIDIGIFIAYLSLAATSQGLGSCILGWFNEKQLQEAL
                     NLPKSEKVRIVIALGYPESDEVKPKKRKSLNQISKFH"
     gene            complement(584..659)
                     /locus_tag="PK087_03540"
     tRNA            complement(584..659)
                     /locus_tag="PK087_03540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(624..626),aa:Arg,seq:cct)
     gene            complement(817..1335)
                     /locus_tag="PK087_03545"
     CDS             complement(817..1335)
                     /locus_tag="PK087_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOH18380.1"
                     /translation="MRTLFVIDTKDYDLNGKVYRRPSTRGIIIRGNKVGMIHSRKYDY
                     YTFPGGGIEPGENQINTLIREVKEETGLEVILGSIKEFGLVHSIQKGKTADEAIFIQD
                     NYYYLCEVRDEWGFPKLDDYESAEQFTFEWIEAMQGIVTNRHHDHGTKNPIMIEREAR
                     VLEILIQEKYLQ"
     gene            complement(1505..1581)
                     /locus_tag="PK087_03550"
     tRNA            complement(1505..1581)
                     /locus_tag="PK087_03550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1545..1547),aa:Arg,seq:ccg)
BASE COUNT          541 a          334 c          281 g          506 t
ORIGIN      
        1 catccgtcgt caacggtcag ccttggtttg ttcatgctgt ttcccaagct gagacgatga
       61 aaaaagtcat caccgcctgt accggcctgg ggatgaaccg ctttattcac caagctcaag
      121 cctttttcgt agttacggaa actcgggaca agctcatttc cacaaccatc agtaaaatta
      181 aaaaactaga ttttcaaacg attgatatcg gcattttcat tgcctactta agtttagcgg
      241 cgacttctca aggtttagga agctgcattt taggttggtt taatgaaaaa cagctacaag
      301 aagctctaaa tcttccgaaa agcgaaaaag tcaggattgt cattgctctt ggttatccgg
      361 aaagcgacga agtgaaaccg aaaaaacgca aaagcctcaa ccaaatcagc aagtttcatt
      421 aatcaatgaa tagtttaaaa taagctttgc ttgaaaaata agcaaaggtt ttttagcaca
      481 tggtcaacaa gcaaaaaaca aattctatgt atcacttatt aaccattagt caaggccaaa
      541 taaaaagcgg tttccaacaa gaaacgcact tagttaagac cgatggcgtc ccaaaaggga
      601 gttgaaccct tgacctattg cttaggaggc aatcgctcta tccactgagc tatcgggaca
      661 agacaagtat aattatacca ttttattgtg cttttttcca ttttttattg gataagtctt
      721 ctttgaatta tgttaaggca tatgattgga ggatgaaact attgataaga aaacaaagaa
      781 acatggttaa ggcttttggt gtttttgttt cctttattat tggagatatt tttcttgaat
      841 aaggatttct aaaactctag cttcacgctc aatcatgata gggttttttg taccatggtc
      901 atggtgacgg ttggtaacaa tgccctgcat cgcttcaatc cattcgaaag taaactgctc
      961 ggctgattcg taatcatcta acttaggaaa accccattca tcccttactt cacaaagata
     1021 ataatagtta tcttgaatga aaatagcctc gtcggcagtt tttccttttt gaatactatg
     1081 caccaaacca aattctttaa tggaaccaag aataacctct aaaccggttt cttctttaac
     1141 ttcgcgaata agggtattaa tttgattttc accgggttca attccaccac ctgggaaggt
     1201 ataataatca tatttgcgac tatgaatcat tcccaccttg ttgccacgaa taatgatgcc
     1261 tcgagtagaa ggtcttcgat aaacttttcc attaagatca taatctttgg tatcaatcac
     1321 aaataaagtg cgcatttttc caccttttaa aaaaacattg agaatattat aacaaattat
     1381 acgcttaaat taaaccataa tcttaacatc ttgccctaat gatttattaa agaagagatc
     1441 taaagccatc ctcccatgag aataataacc gcattcaaaa ccaaatgcgg ttatttgtta
     1501 gcaatggtgt cctgaaaggg actcgaaccc atggcctatt gcttcggaag caatcactct
     1561 atccaactga gctatcagga caaaacataa atatattata ctcctaaacg aataattttt
     1621 ccaccttttt ttccaatcat caccgaagat gtcgattagc tt
//
LOCUS       DAOSGN010000225         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_75823_length_1652_cov_2.207890, whole genome shotgun sequence.
ACCESSION   DAOSGN010000225 DAOSGN010000000
VERSION     DAOSGN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75823_length_1652_cov_2.207890"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK087_03555"
     CDS             complement(<1..634)
                     /locus_tag="PK087_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18381.1"
                     /translation="MMLLMLSGNMVFALPDIFPRLNIIKSSKEIYDKITATETHTNPN
                     NRPHDFKFQKELKVSNLSFAYDDKDLFHNVSFTIEKGKKYLLKGASGVGKSTLIKLLS
                     QTYPNYEGSISLDGIDLKTIKDKSFASKVAYIYQEVFLFEDSIKNNITLFKELDQKTI
                     DEAIIKSGLQEFIQSRKQGLNEKLSENGKNLSGGERQRLSIARAIAKKAEI"
     gene            complement(673..1434)
                     /locus_tag="PK087_03560"
     CDS             complement(673..1434)
                     /locus_tag="PK087_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOH18382.1"
                     /translation="MKDLLFKRKGRFFLYFIACFFPVVSDLLQTYIFSKLFDAIAKLD
                     PKYFFQVSLMAGAYVIIGALLYITSRLLRISYMRDTILDVRIAAFSKIMAYSYQQFSQ
                     KSKDTYLSNLINDINNFENNFFINLLNFIFRIGMYLGSLIIMLIIDYRLASAMVVFSF
                     ITFFLIRAFEKKTVALQKQVSKTNEDFTLNIGNTFNGLEILKLNNMDHKFLAKSFEAI
                     NKVEQSKYRFRFFGDSQRNLTQGIAGASTVAVLFI"
BASE COUNT          521 a          275 c          337 g          519 t
ORIGIN      
        1 agatttccgc ttttttcgca atcgctctgg caatcgacaa acgttggcgt tcaccgccgg
       61 agaggttctt accgttttcg cttagttttt catttagtcc ttgcttgcga ctttggataa
      121 attcttgcaa gccggattta atgatggctt catcaatcgt cttttggtcg agttctttaa
      181 acaaagtaat attattttta atgctatctt caaacaaaaa cacttcctga taaatatagg
      241 cgactttgct ggcaaaactc ttgtctttga tggtttttaa gtcaatgccg tctaagctaa
      301 ttgaaccttc atagttggga taggtttgcg acaaaagctt gataagcgtg gatttcccca
      361 ccccgctggc cccctttaat aagtattttt tacctttttc aatggtaaag gaaacattgt
      421 gaaagaggtc tttatcatca taagcaaagc ttaggttgct gactttcagt tccttttgga
      481 acttaaagtc atggggacga ttattaggat tggtatgtgt ttccgtggcg gtaatcttat
      541 cgtaaatttc ttttgatgat tttatgatat ttaatctcgg gaagatatcc ggaagagcaa
      601 agaccatatt ccccgaaagc atcaataaca tcatcaattg gtcgtattgg agaccaaatt
      661 tagtttgata gattaaataa agaggacggc cacggtactg gctccggcaa tcccttgggt
      721 aaggttgcgt tgggaatccc caaagaaacg gaaacgatat ttgctttgtt cgactttgtt
      781 aatggcttca aaacttttcg ccaggaactt atgatccata ttgttcaatt ttaagatttc
      841 gagaccgtta aaagtattac cgatattgag ggtgaagtct tcgttagttt ttgacacttg
      901 cttttgcaaa gcgacggttt tcttttcaaa agcacggatt aagaagaaag tgatgaagga
      961 aaagaccacc atggcgctag cgagccgata atcaatgatt agcataatga ttagggaacc
     1021 aaggtacatc ccaattcgaa aaatgaagtt taataagtta atgaagaaat tgttttcaaa
     1081 attgttgatg tcattgatga ggttcgaaag ataagtatct ttcgactttt gactaaactg
     1141 ttggtaggaa taagccatga ttttggaaaa agcggcgatt ctgacatcaa gaatggtatc
     1201 acgcatataa gagatgcgta gtagccttga agtaatataa agcaaggcgc caatgatgac
     1261 ataagcacca gccatgaggg atacttgaaa aaagtatttg gggtcaagct tggcaatcgc
     1321 atcaaataac ttggaaaaga tataggtttg taaaagatcg ctcactaccg gaaagaagca
     1381 agcgatgaag tagaggaaga agcgcccctt tcttttaaac aataagtctt tcataggaaa
     1441 atccctttca aaatggaaat acgctttatt atatatcgac taaattaatt tgtcaataaa
     1501 caaaattaat tactttatta aataaattaa taatttgttt aaaataaaga agagcttaag
     1561 tcaaaaactt aagcccgcag attatttttt tgatttttca tagtcttgca agaagcggtc
     1621 aatgccaaca ttcgtgagtg ggtggttaaa ca
//
LOCUS       DAOSGN010000226         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_76567_length_1641_cov_4.106557, whole genome shotgun sequence.
ACCESSION   DAOSGN010000226 DAOSGN010000000
VERSION     DAOSGN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76567_length_1641_cov_4.106557"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PK087_03565"
     CDS             complement(<1..248)
                     /locus_tag="PK087_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOH18383.1"
                     /translation="MDNKLPVLTTSRLILREISDNDYLDMFEYAKIPYVGPAAGWQPH
                     KSVAETKAVIKMFQGKRKYGQLGVFAIVLKSTNKMIGT"
     gene            complement(261..1037)
                     /locus_tag="PK087_03570"
     CDS             complement(261..1037)
                     /locus_tag="PK087_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOH18384.1"
                     /translation="MNFLIIGDVFSKLGRKSLEYNLKKLRETEAIHFVIVNGENTSHG
                     RGLNEGHYKWLLEQGIQVITLGNHSFSNRSIYKYIDEAKNLVRPANFSDDAKGQGYVT
                     IKYNQLSVTVMQVVGQVFMNGEVVSPFLKAQEILDQVPSDIYVCDFHGEATSEKIAFG
                     YHFDGKIQIIFGTHTHVQTNDARLLDQGTAFITDVGMTGPLDGVIGVEKRIILDRYLN
                     NGLERFSPQETGKTQFSAVLVEVNEITKKASKIKTIRIID"
     gene            complement(1247..>1641)
                     /locus_tag="PK087_03575"
     CDS             complement(1247..>1641)
                     /locus_tag="PK087_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181928.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOH18385.1"
                     /translation="NRFRENPVVIDAIASHHGDNEPTSFISQLVVAADTMSAARPGAR
                     SESIENYIKRLSKLEEICNEFKGVDKAYAIQAGREIRILVKPDEINDVEAHKLARDIR
                     MKVEGNVNYPGTIKVTVIRETRVHEIAK"
BASE COUNT          465 a          345 c          291 g          540 t
ORIGIN      
        1 atcgtcccaa tcattttgtt ggtgctcttt aagacaatgg caaaaacgcc gagttgaccg
       61 tattttcttt tgccttgaaa cattttaatc acagcttttg tttccgcaac tgatttatgc
      121 ggttgccaac cggcagccgg ccccacatag ggtattttcg catattcaaa catatcaaga
      181 tagtcgttgt cgctaatttc tcgtaagatt aagcgactcg tagtaagaac aggtaattta
      241 ttgtccataa gcttttcctt ttaatcaatg attcggattg tctttatttt gcttgctttt
      301 ttggtaattt cgttcacttc cacgagcaca gcggaaaatt gggtttttcc tgtttcttgg
      361 ggagaaaatc tttccagtcc attatttaaa tagcggtcta aaataattct tttttctaca
      421 ccaatcaccc catccaaagg ccccgtcatc cccacatcgg taataaaggc ggttccttgg
      481 tcaagaaggc gggcatcgtt ggtttgaaca tgggtgtgag tgccaaaaat aatctgtatt
      541 ttgccatcaa aatgataacc gaaagcaatt ttttcgctgg tggcttctcc atgaaaatca
      601 caaacataaa tatcacttgg tacttgatcc aagatttctt gcgcttttaa aaagggtgaa
      661 acaacttcac cattcataaa aacctgacca accacttgca taacggtaac gcttaattgg
      721 ttatatttga tggtgacata accttggccc ttggcatcat cgctaaagtt ggcaggacga
      781 actaagtttt tggcttcatc aatgtattta taaatactgc gattgctgaa agaatggttt
      841 cctaaagtaa ttacttgaat accttgttct aaaagccatt tataatgacc ttcatttaga
      901 ccgcgaccat gggaagtatt ttcaccgtta acaataacaa aatgaatcgc ttcggtttct
      961 ctcagttttt ttaaattgta ttctaaactt ttacggccta gtttcgaaaa aacatcaccg
     1021 atgattaaga aattcatgtt gataccaacc ttttgttaat tattatatca tagactatgg
     1081 gctttcacaa gacaagcaac ttaaaattta catgcctgtg ggatgacaat atctttgaac
     1141 ttttaggaaa ttaattactt aaccaacaaa aaaggaatat gctgacatac tccttaattg
     1201 gctttttgat gattcgttgt ccgtccacaa tcgacaactc ctcttcttat ttggcaattt
     1261 catgaactct cgtttcccgg atgaccgtca ctttgatggt tcccggatag ttgacattgc
     1321 cttcaacttt cattctgata tcacgagcta gtttatgtgc ttcaacatca ttaatttcat
     1381 ccggcttaac aagaatgcga atttcccttc cggcttgaat cgcataagcc ttatcaacac
     1441 ctttaaactc gttacatatt tcttctaatt tgcttaaacg cttaatgtaa ttttcaattg
     1501 attcgctacg ggcccccggt cgggcggcag acatcgtatc ggcagcaacc actaattggg
     1561 aaataaacga cgttggttcg ttatcaccgt ggtgggaagc aatagcatca atgacaaccg
     1621 gattttcacg gaaacggttg g
//
LOCUS       DAOSGN010000227         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_77285_length_1631_cov_2.353426, whole genome shotgun sequence.
ACCESSION   DAOSGN010000227 DAOSGN010000000
VERSION     DAOSGN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77285_length_1631_cov_2.353426"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /gene="fusA"
                     /locus_tag="PK087_03580"
     CDS             <1..1229
                     /gene="fusA"
                     /locus_tag="PK087_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH18386.1"
                     /translation="PTDVPAISGHRDGKEVIIKSTDDGYFTALAFKVMTDPFVGKLTF
                     FRVYSGTLKSGSYVYNSTKGQKERVGRILRMHANDRIEIKEVFAGDIAAAVGFKDTTT
                     GDTISDEKFPVILESMIFPEPVISVAIEPKTKVDQDKMGVALQKLAEEDPTFKTYTNS
                     ETGQTIISGMGELHLEIIVDRMRREFKVEANVGMPQVSYRETITTKAEVSGRFVRQSG
                     GRGQYGDVKIRFEPNPGKGFEFVDAIIGGVVPKEYIPAVKKGLENSLTNGNLAGYPTI
                     DIKATLSDGSYHEVDSSQIAFEIAASYAFRETKDKCQPVLLEPIMAVEVVVPDDYLGN
                     VIGDLTGRRGRIEGQDSHNHLHSVKALVPLSQMFGYATALRSNTQGRGNYTMQFSHYE
                     ECPKSFAELITKKRNS"
     gene            1309..>1631
                     /locus_tag="PK087_03585"
     CDS             1309..>1631
                     /locus_tag="PK087_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOH18387.1"
                     /translation="MAKAKFERTKVHVNIGTIGHVDHGKTTLTSAITKVLAKKGLSKV
                     LEYDAIDSAPEEKARGITINTSHVEYETANRHYAHVDCPGHADYVKNMITGTAQMDGA
                     ILIMTV"
BASE COUNT          505 a          284 c          369 g          473 t
ORIGIN      
        1 ctccgaccga tgttccggcg attagcggtc atcgtgatgg gaaggaagta atcattaaat
       61 caactgatga cggatatttt acagctttgg catttaaggt tatgacggat ccgtttgttg
      121 ggaaattgac ctttttccgt gtttattccg ggactttaaa atccggatcc tatgtctata
      181 actcgaccaa ggggcagaaa gaacgtgttg gtcgcatctt aagaatgcat gccaacgatc
      241 gcatcgaaat taaggaagtt tttgcgggtg atattgctgc cgccgtcggt tttaaagata
      301 caacgaccgg cgatactatt agcgatgaaa aattccccgt tattcttgaa tcgatgattt
      361 tcccggaacc ggttattagt gtagctattg aaccgaaaac taaggttgac caagataaaa
      421 tgggtgtcgc tttacagaaa ttggcggaag aagatcccac ctttaagact tataccaatt
      481 cagaaactgg gcaaacgatt atttctggta tgggtgaact acatttggaa ataattgttg
      541 accgtatgcg gcgagaattt aaagttgaag ccaatgtggg gatgccgcaa gtctcttatc
      601 gggaaactat taccacaaaa gcggaagtgt ctgggcgctt tgttcgtcaa tctggtggtc
      661 gtggtcaata cggtgatgta aaaatccgct ttgaacctaa tcccggcaaa ggtttcgaat
      721 ttgttgatgc aattatcggt ggtgttgttc cgaaagaata tattccggcg gtgaaaaagg
      781 gtttggaaaa ttccttaaca aatggtaatt tagccggcta tccaaccatt gatatcaagg
      841 cgactctctc tgatgggtct tatcatgaag tggattcaag tcaaattgct tttgaaatcg
      901 cggccagtta tgcttttcgc gaaacgaaag acaaatgtca accggtttta ttggaaccga
      961 ttatggccgt cgaagttgtt gttccggatg attatttagg aaatgtgatt ggtgatttaa
     1021 ctggtcgaag aggccgtatt gagggtcaag atagccataa tcatttgcat agtgtcaaag
     1081 ctttggttcc gctatcccaa atgtttggtt atgcaacggc tttgcgaagc aatactcaag
     1141 ggcgaggaaa ttatacaatg caattttctc actatgaaga atgcccgaaa tcgtttgcgg
     1201 aattaatcac aaaaaaacgc aattcataaa taattaattt tatgcttgca aaaaaaagac
     1261 aattattata atatatatat agaaaaaata ttaggaggaa aaaataaaat ggcaaaagct
     1321 aagttcgaac gtacaaaagt acacgttaat attggaacca ttggtcatgt tgaccatggt
     1381 aaaacaacat taacatctgc tattactaaa gttttagcaa aaaaaggcct ttccaaagtt
     1441 ttggaatatg atgcaattga tagtgctccc gaagagaaag cacggggaat tacgattaat
     1501 acttcccatg ttgaatatga gactgctaat cgtcactatg ctcatgttga ctgcccggga
     1561 catgctgact atgtaaaaaa tatgattacc ggtactgcgc aaatggatgg cgccattctt
     1621 attatgactg t
//
LOCUS       DAOSGN010000228         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_77870_length_1623_cov_2.951531, whole genome shotgun sequence.
ACCESSION   DAOSGN010000228 DAOSGN010000000
VERSION     DAOSGN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77870_length_1623_cov_2.951531"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..>1623)
                     /locus_tag="PK087_03590"
     CDS             complement(223..>1623)
                     /locus_tag="PK087_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH18388.1"
                     /translation="KVFEHIGGYFPFVVDITPYLQEDNEIIVQVTDPSNHSFYAYGKQ
                     HTKPKGIWYTPVSGLWQSVWLEAVADDYLHDLFITPDFDHSSVHFDLVGLKEVSGTIT
                     IFANKKIVAQEKINKASFDIVLPNFIPWTMAHPHLYQVLIELASDTVETYFGMRKLTI
                     GDGQFGKALFLNNQEIFLSGVLDQGYYPDGLYTPAVDQAYIDDIKLMKSMGFNVLRKH
                     AKIEPRRFYYHCDRLGMLVWQDMVNGGRYSFPHMSVLPLLGFLSRSDQKRLKIFGRKS
                     LISQESFRKEMEATVNLLRNAPSLVIWTIFNEGWGQFKSQENYQFLKTLDQSRLIDAA
                     SGWFDQGIGDFASKHIYFKKITFKKDVRPLVLSEFGGYSYKVPKHSYHLTKEFGYRKY
                     PSLDTWQQAVVKLYETEIIPQISKGLCATIYTQLSDVEEETNGLITYDREFVKMDGKI
                     MKQINERLNAQFSKKH"
BASE COUNT          498 a          331 c          301 g          493 t
ORIGIN      
        1 aaattttccg caacatttct cacaacaccg ccccaacttt tggtagaaaa aaccgggttg
       61 gaagtgttta acatgagcat gtgatgtaag tgcaatttaa tatcaatatt ggcgccccca
      121 aggcataaaa ttttcggcct ttctttcata agcatctcca tctttgatag aatcattata
      181 tcatattctt tagattttag gtaaaaaatc aaaaaaagca catcagtgct ttttactaaa
      241 ttgggcatta agacgctcat taatttgctt cataattttc ccatccattt tcacaaactc
      301 tcggtcataa gtaattaaac cgttggtttc ttcctcgaca tcacttaact gggtataaat
      361 agtggcacat aaacccttgc ttatttgggg gatgatttcc gtttcatata acttcactac
      421 cgcctgttgc caagtatcta aagaaggata tttacgatag ccaaattctt tagttaagtg
      481 ataagagtgc ttaggaactt tataagaata accgccaaac tcgcttaaaa caagggggcg
      541 aacatctttt ttaaaagtga tttttttaaa ataaatatgt ttgctggcaa aatcgccaat
      601 accttggtca aaccaaccgc tggcagcatc gattaatcgg ctttggtcaa gcgtttttag
      661 gaattgataa ttttcttggc ttttaaactg gccccagcct tcattaaaaa tagtccagat
      721 aactagcgag ggcgcgttcc taagtaaatt tacagtcgct tccatttctt tgcgaaagct
      781 ttcttgggat attaatgatt ttcttccaaa gatttttagc cgcttttgat cactccggga
      841 taaaaaacca agtagcggta gaacggacat atgggggaag gaataacgcc ccccgttaac
      901 catatcctgc caaaccagca ttccgaggcg gtcacagtga tagtaaaaac ggcgcggttc
      961 aattttcgca tgttttctta aaacattgaa acccatgctt ttcataagtt tgatatcatc
     1021 aatataggct tgatcaacgg ccggagtata gaggccgtcc ggatagtatc cttggtctaa
     1081 aaccccgctt aaaaagattt cctgattatt taaaaacaag gctttcccaa attggccatc
     1141 gccaatagtc aatttgcgca tcccaaaata agtttcgacc gtatcgctgg ctaattcgat
     1201 taatacttga taaagatggg gatgagccat cgtccaggga ataaaattgg gaaggacaat
     1261 atcaaagctg gctttattaa ttttttcttg ggcaactatt ttcttattag cgaatattgt
     1321 aatcgttccg ctgacttctt ttaatcccac caaatcaaaa tgaacggaac tgtgatcaaa
     1381 atccggggta ataaataaat catgaagata gtcatccgcc accgcttcca accagacgga
     1441 ttgccaaagc ccgcttaccg gagtatacca aatgcccttt ggcttggtat gttgttttcc
     1501 gtaggcataa aaactgtggt tgctggggtc agtaacctga acgatgattt cattatcttc
     1561 ttgtaaatag ggagtaatat caacaacaaa agggaagtag ccaccgatat gttcaaagac
     1621 ttt
//
LOCUS       DAOSGN010000229         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_78466_length_1615_cov_3.414744, whole genome shotgun sequence.
ACCESSION   DAOSGN010000229 DAOSGN010000000
VERSION     DAOSGN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78466_length_1615_cov_3.414744"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PK087_03595"
     CDS             <1..632
                     /locus_tag="PK087_03595"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH18389.1"
                     /translation="IVNEYGADTLRLYEMFMGPLEAVKPWSTAGVEGPRRFLERVYRF
                     YTEVANFDANNFVLEKLYHQTIKKVTEDYTNLRFNTAISQMMIFMNECYKNPNIPLEF
                     LEGFLKLLNPIAPHITEELHEMVLNGRESLANLPWPTFDENKIKEDEITFVVQVNGKI
                     RDKITVNAEVSDQEMEKLALQSEKVQTFINGLTIRKIIVISKKIVNIVV"
     gene            701..1030
                     /locus_tag="PK087_03600"
     CDS             701..1030
                     /locus_tag="PK087_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18390.1"
                     /translation="MNCLVKRSFLNMETVFRCDRCEHFVLDEQIIVGNKTIGIYHIFP
                     GCRLLKDLTNDQIAHSQRLKLLLKDAIIFQEQIVLFYEESLSFNRLLDVFSAFLDAGY
                     FVQIITL"
     gene            1107..>1615
                     /gene="raiA"
                     /locus_tag="PK087_03605"
     CDS             1107..>1615
                     /gene="raiA"
                     /locus_tag="PK087_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOH18391.1"
                     /translation="MKVIVRGKNKFVPSDAIKQYATEKLQKLEQYFSSRENLEANVLC
                     KVYESYQTVEITIPTKNVLLRAEVKNQTIYGAIDLAIDKLETQIRKHKSKIYKSMKHR
                     SGVGQFYASQSDFDVEKMKTEILSTNLVKSKQVDLQPMTPDEAILQMEMLGHDFFVFM
                     NSETKKVSVV"
BASE COUNT          510 a          280 c          310 g          515 t
ORIGIN      
        1 acattgtcaa tgaatatggc gcggacactt tacgcttgta tgagatgttc atgggacctc
       61 tcgaagccgt caaaccgtgg tcgactgctg gagtggaagg accgcggcgt ttcttggaac
      121 gcgtttatcg tttctatacg gaagtcgcca attttgatgc taataatttt gttttagaaa
      181 aactatacca tcagaccatc aaaaaagtta cggaagatta taccaacttg cgttttaata
      241 ccgccattag ccaaatgatg attttcatga atgaatgtta taaaaacccc aacattcctt
      301 tagagttttt ggaaggtttc ttaaaattat tgaatccgat tgccccccat attaccgaag
      361 aacttcatga aatggtcttg aatgggcggg aatcgttggc taatttacca tggccgactt
      421 ttgatgaaaa caagataaag gaagatgaaa ttacttttgt cgttcaggtt aacggtaaaa
      481 tccgtgataa gattacggtt aatgccgagg tcagcgatca agaaatggaa aagttagccc
      541 tccaaagtga gaaagtccaa actttcatta acggtttaac aatccggaaa atcattgtca
      601 tctccaagaa aatcgttaat atcgttgttt aagcaaacca catggtttgc ttttttaaag
      661 gattttgctt ttttttggat aatatatcat tggtgatagg atgaattgtt tggtaaaacg
      721 aagcttttta aatatggaaa ctgtttttcg ctgcgatcgc tgtgaacatt ttgtgcttga
      781 tgaacaaatc attgtcggta ataaaaccat tggcatttat catatctttc ccgggtgccg
      841 cttgcttaaa gacctcacca atgaccaaat cgctcattct cagcgtttaa agttgctttt
      901 gaaagatgcg attatttttc aagagcaaat cgttcttttt tatgaggaaa gtttgtcttt
      961 taatcgcttg ttagatgttt tttccgcctt tcttgatgct ggttattttg tacaaataat
     1021 tacgctttaa aacgcttatt tatttttaca tatctttttt tatgtggtat aattaaggcg
     1081 taaacttaag caaaggagat gattttatga aagtaattgt acgtggcaaa aacaaatttg
     1141 tgccgtcgga tgcgattaaa cagtacgcaa ccgaaaaatt acaaaaactc gaacaatact
     1201 ttagttcccg ggagaatcta gaagctaatg ttttgtgcaa ggtttatgaa agttaccaaa
     1261 ctgtggagat taccattccc acgaagaatg tgttattaag agcggaagtc aagaatcaaa
     1321 ctatttatgg ggccattgac ttagccattg acaaactaga aacccaaatt cgaaaacaca
     1381 aatccaaaat ttataaatcc atgaaacatc gttctggggt cggccaattt tatgcttccc
     1441 aatccgactt tgatgttgag aaaatgaaaa cggaaattct ttcgaccaat ttagtcaaat
     1501 ctaaacaagt cgatttacaa ccgatgactc ccgatgaagc aattctccaa atggaaatgc
     1561 tcggtcatga tttctttgtt tttatgaaca gcgaaactaa aaaagttagc gttgt
//
LOCUS       DAOSGN010000230         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_78959_length_1609_cov_2.429858, whole genome shotgun sequence.
ACCESSION   DAOSGN010000230 DAOSGN010000000
VERSION     DAOSGN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78959_length_1609_cov_2.429858"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PK087_03610"
     CDS             <1..696
                     /locus_tag="PK087_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014651237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH18392.1"
                     /translation="LLIINTFILAVVTGPVSYILAFVFAWLIHELPRKIRWLMTLVFY
                     APSISGSAYLLWQLIFSSDMYGVLNATLIHLGVINAPILWLETPRYALPILIIVQLWL
                     SLGVTFLAFIAGLQTVDRTLYEAAAIDGLRNRWQELWFITLPQMKSQLMFGAVMQITS
                     ALGIAAVSINLLGFPSVEYSGHTIVTHLMDFGSTNSTRLEMGYASAIATVLFFIMLGS
                     NLIVQKIIRRVGS"
     gene            693..>1609
                     /locus_tag="PK087_03615"
     CDS             693..>1609
                     /locus_tag="PK087_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH18393.1"
                     /translation="MRKKTATPLVNEQERSQMIQDLKVQELEWYKQKRKFKKYRRVRL
                     SRSWVGDIFVVLVLVLFGSFSAYPLVYTISNSLKGLDEIFVFPPRLFPRNVTFRNFVD
                     LFNLIGNTQIPISRYFINTLTITLFGTAGHVLLSSLCAYPLSKHRFPGRHLINQLVVY
                     SLMFSAAVTAIPSYMIISWLGLIDTQLAIIIPAFGFTLGLFLMRQFMNVIPNELLEAA
                     KIDGASEYQIFFRIVMPLVKPAWLTLIILLFQQLWGTDGGSYIFTENLKPLSYALSQI
                     VAGGVARTGTAAAVTVIMLIVPISVFIFN"
BASE COUNT          394 a          326 c          329 g          560 t
ORIGIN      
        1 ttattaatca taaacacctt tattttagca gttgttaccg gtccggtatc atatatccta
       61 gccttcgtat ttgcttggct catccatgaa ttgccaagaa aaattcggtg gttgatgacc
      121 ctagtcttct atgctccttc catcagtggc agtgcgtatt tgttgtggca gttaattttt
      181 tcttccgata tgtatggggt cttaaatgca acgctcattc atctaggtgt tattaatgcg
      241 ccgattttat ggttggaaac accacgatat gctttaccga ttttaattat tgtccagttg
      301 tggttaagct taggagttac tttcttagct tttatcgccg gcttacaaac ggttgaccga
      361 actttgtatg aagcagcggc cattgacggt ttacgcaacc gatggcagga attatggttc
      421 atcactcttc cccaaatgaa atcacagtta atgtttgggg cggttatgca aattaccagc
      481 gccttaggca ttgcggctgt ttccatcaat ttactgggct ttccatcggt ggaatattcc
      541 ggtcacacca ttgtgaccca tttaatggat tttggttcca ctaattccac aagattggaa
      601 atgggttatg cttcagccat tgcgacagtg ttatttttca ttatgctggg gtcaaaccta
      661 attgttcaaa aaatcattag gagggtcgga tcatgagaaa aaagacggct actccgcttg
      721 ttaatgaaca agaacgctcg caaatgattc aagatttaaa agttcaggaa ttagaatggt
      781 ataaacaaaa acgaaaattt aaaaaatatc gtcgggttcg cttaagtcgt tcttgggttg
      841 gtgatatctt tgtggtgctt gttttagtgt tgttcggctc tttcagcgcc tatccgcttg
      901 tttacacgat ttccaactcc ttaaaagggc ttgatgaaat ctttgttttc ccccctcgcc
      961 tttttcctag gaatgttacc tttcggaact ttgttgattt gtttaactta atcggtaata
     1021 cccagattcc gatttctcgt tactttatta ataccttaac gattacgttg tttgggacag
     1081 ctgggcatgt attactttcc tccctatgtg cttatccgct gtccaaacac cgttttccgg
     1141 gtcggcattt aattaatcaa ttagttgttt attcattgat gttttcggcg gcggttaccg
     1201 ctattccttc atatatgatc attagttggc tcggccttat tgatacccaa ttagccatta
     1261 tcattcccgc tttcggtttt actttaggat tattcctcat gcgccaattt atgaatgtca
     1321 ttcctaacga attactggaa gctgccaaaa ttgacggtgc cagtgaatac caaatctttt
     1381 ttagaatagt gatgccattg gttaaaccgg catggttaac tttgattatt cttttgtttc
     1441 aacaattatg gggaaccgat ggcggttcat atatctttac ggaaaattta aaacccttaa
     1501 gctatgccct tagtcaaatt gtcgccggtg gcgtcgcaag aacagggacg gcggcggcgg
     1561 tgacagtcat tatgttaatc gtgccgattt cggtcttcat ctttaacca
//
LOCUS       DAOSGN010000231         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_79113_length_1607_cov_2.773196, whole genome shotgun sequence.
ACCESSION   DAOSGN010000231 DAOSGN010000000
VERSION     DAOSGN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79113_length_1607_cov_2.773196"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PK087_03620"
     CDS             complement(<1..832)
                     /locus_tag="PK087_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOH18394.1"
                     /translation="MNPNFEEKDYSDEKFSLRIWKKIFKVVLKKKKSLIIMVLSVLGL
                     SLLDLAYPLLNAYAIDHYFKTANPDFSNKYFFLAAYALIAMGFAMTVFFYIKAAGEVE
                     VEVGYELRDEAFYRLQQLPFSYYDKTPSGWIMARLTSDSRRLAMILSWGLVDMIWGGL
                     SMIGILIVLFIVNWKLAFILLALLPVLLVVSIYFRKKILAAYRNVRKTNSKITASYNE
                     GIMGNKTTKTLVLEDFKLQEFDDLCVNMRKQSIRAIINSAIFFPIIIVLSYVAVVLIL
                     R"
     gene            complement(822..>1607)
                     /locus_tag="PK087_03625"
     CDS             complement(822..>1607)
                     /locus_tag="PK087_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18395.1"
                     /translation="QVTGDIVFENVGFKFPDTDKHLLNGVNFTINPGETVAIIGRTGS
                     GKSTIANLLVRMLDYESGSIRINGVELKTIKKQWIRQQIGIILQDPFLYATTIYENIK
                     IGIKDINPEKIYSAAQTAAIDKDIKQFEKGYDTLVGEKGVTLSGDQKQRVAIARMLVL
                     DKPVMIFDDSLSAVDTTTDLMIRNSLKERNQKLTSLIITHRITTAKEADKIIVLENGI
                     VTAIGKHEELVHQEGLYQKLWSIQGALESEFVKIVEGGEEHES"
BASE COUNT          486 a          342 c          307 g          472 t
ORIGIN      
        1 ttcttaaaat gaggactacc gcaacataac tcaaaacaat gataatcgga aagaaaatgg
       61 cggaattaat gatggctcta atcgactgtt tgcgcatatt gacgcataaa tcatcaaatt
      121 cctggagttt aaaatcttct agcaccaagg ttttggtcgt tttgttgccc atgatgcctt
      181 cgttgtaaga agcagtaatc ttcgaattcg tttttcgcac attgcggtaa gcggcgagga
      241 ttttcttccg aaaatagata ctgacaacca gcaataccgg cagtaaagct aaaagaatga
      301 aagccagttt ccagttaaca atgaagagca caatgagaat gccaatcatc gacagcccgc
      361 cccaaatcat atcaacgagc ccccaagaaa gaatcatcgc cagtcggcgg gagtccgagg
      421 ttaagcgcgc cataatccat cccgatgggg ttttatcata ataagagaac ggtaactgct
      481 gtaagcggta aaaagcttca tcgcgaagtt cgtaaccgac ttccacttcc acttcccccg
      541 cagctttgat gtaaaaaaag acggtcatcg caaagcccat ggcaataaga gcataagcgg
      601 ctaaaaagaa atacttgttg gaaaaatcag gattagcggt tttaaaataa tgatcgatag
      661 cataggcatt aagaagggga taagctaaat caagtagact cagtccaaga acggacaaga
      721 ccataatgat taaactcttc ttctttttta agaccacttt aaaaatcttt ttccaaatgc
      781 ggaggctgaa cttttcatca ctgtagtctt tttcctcaaa attaggattc atgttcttcc
      841 cccccttcca caatcttcac aaattcgctt tccaatgcgc cttgaattga ccatagtttt
      901 tgatataggc cttcttggtg aacgagttct tcatgcttgc caatggcggt cacaatacca
      961 ttttccaaaa caataatctt gtccgcttct ttggcggtgg taattcggtg ggtaatgata
     1021 agagaagtta atttttggtt tcgttctttc agtgagtttc taatcattaa atcagtggtc
     1081 gtatcaacgg cacttaagga atcatcaaaa atcatcaccg gtttatccaa aacaagcatc
     1141 cgcgcaatgg caacacgttg tttttggtcg ccggataatg tgacgccttt ttcaccgact
     1201 aaagtatcat aacctttttc aaattgcttg atatctttat caatcgctgc tgtttgggca
     1261 gcactgtaga ttttttccgg gttgatatct ttgatgccga ttttgatatt ttcataaata
     1321 gtcgttgcat acaaaaacgg atcttgtaag ataatgccga tttgttggcg aatccattgt
     1381 tttttaatcg tttttagttc aacaccgtta atccgaatac tgcccgattc ataatccagc
     1441 attcgcacta ataaattagc aatcgtcgat tttccacttc ccgtccgacc aataatggcg
     1501 acggtttcac cgggattgat ggtgaaatta accccgttca ataaatgttt atcggtatcg
     1561 gggaatttaa agccaacatt ctcaaaaaca atatcaccag tgacttg
//
LOCUS       DAOSGN010000232         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_79322_length_1604_cov_2.637185, whole genome shotgun sequence.
ACCESSION   DAOSGN010000232 DAOSGN010000000
VERSION     DAOSGN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79322_length_1604_cov_2.637185"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PK087_03630"
     CDS             complement(<1..1104)
                     /locus_tag="PK087_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH18396.1"
                     /translation="MRKFSKLVVLCLTLVMALFLASCKEKIELSFENETMTMIVGDTK
                     TLVPVANKEGLTYEWTSDKTAVVTVDAGLVTAVSAGTAKVTVKVKDKDVSATITITVN
                     PVVISFTEKTMTIAIDQSVTLTPTVVPAQTMTYTWTTSDASVATVTNGVVKGIKVGTA
                     TVTASGKGGSASITINVVLPNPTQVTITGASASAKVGATMQLGATILPAKASQAVTWK
                     SSDTKLATVSASGLVTFVGVGKVTITATSVALDSQKASVTIDVGAPDPTKVAITGAST
                     TAKVGDKMQLSATVLPATALQTVAWKSSDTTLATVTPAGLVTFLGMGSVTITATSTVL
                     NTLKATVTIEVGAPDPASIAVTGTDGKDRVIIGE"
     gene            complement(1288..>1604)
                     /locus_tag="PK087_03635"
     CDS             complement(1288..>1604)
                     /locus_tag="PK087_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18397.1"
                     /translation="KIEASQKLPGAIESNVTYFNGNDALVLKKGDVVIDIIGLVGNDP
                     GTNWAVGVGATSEYTLVRKSSYAGLPLTVFAPEQWDVYPQNYFGNMDSHAYLPAYVAP
                     AS"
BASE COUNT          442 a          383 c          312 g          467 t
ORIGIN      
        1 ttcaccaata ataactcgat ccttgccatc agtgccggta acagcaatgc ttgctggatc
       61 gggagcgcca acctcaatgg tcactgttgc tttaagagta ttcaaaacgg tagatgtcgc
      121 ggtaatggta acacttccca tgccaaggaa agtcactaaa ccggctggtg taacagttgc
      181 caatgtggta tcacttgatt tccaagcaac agtttgtaaa gctgtagccg gtaataccgt
      241 agcgctaagt tgcattttat cgccaacttt agcggttgta gaagccccgg taatcgctac
      301 tttagtcgga tcaggagccc caacatcaat ggtcacactt gccttttggc tatccaaagc
      361 gaccgatgtt gccgtaatgg tcactttacc aacaccgacg aaagtcacta aaccgcttgc
      421 ggaaaccgtt gccagcttag tatcacttga tttccaggta accgcttggg aagctttcgc
      481 tggtaagatc gttgctccaa gttgcatagt agcgccaact ttagcacttg ccgatgcccc
      541 ggtaatcgtt acttgggtcg gattaggtaa gacaacatta attgtaatgg aagcacttcc
      601 acccttcccc gatgctgtaa ccgtcgctgt tccgactttt attcccttaa caacaccgtt
      661 agtaaccgtc gccacacttg catcgctagt tgtccaggta taagtcatcg tttgggctgg
      721 cacaaccgtt ggcgttaaag tcacgctttg atcgatggca attgtcatcg ttttttccgt
      781 aaaactaatg acgacaggat taaccgtaat ggtaatggtc gcactgacat ctttgtcttt
      841 aaccttgaca gttactttag ccgtccccgc actaacagcg gtaacaagcc cggcatcaac
      901 cgtaacaact gcggttttat ccgacgtcca ttcataagtt aaaccttctt tgttggccac
      961 cggaactaat gttttcgtat caccaacaat catcgtcatc gtttcgtttt caaagcttag
     1021 ttcaattttc tctttacaag atgctaagaa aagagccatc accaaggtca agcatagaac
     1081 gactaatttt gaaaatttac gcatacattt ctaataactt aaacccttgc atactgacgg
     1141 gcttaaatta ctttcctcca cttttatttt tgtcagtatt atttttccgt atcaattata
     1201 acatattaaa tcttagataa ctacttattt tctttcaata aaaaaagtcc caaaaggctt
     1261 accttttagg actagtcttg agcatgatta tgaagccgga gctacataag ccggtaaata
     1321 ggcatggcta tccatattcc cgaaataatt ttgcgggtaa acatcccatt gttccggagc
     1381 aaagacggtt aacggaagtc cagcataaga agatttacga actagggtgt attccgaagt
     1441 tgcaccgaca ccaaccgccc agttagttcc tggatcattg cccactaaac cgataatatc
     1501 aattacgaca tcgccttttt tcaagacaag ggcatcgtta ccattgaagt aagtaacatt
     1561 gctttcgata gctcccggta atttttggga agcctcgatt ttat
//
LOCUS       DAOSGN010000233         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_79995_length_1595_cov_2.405195, whole genome shotgun sequence.
ACCESSION   DAOSGN010000233 DAOSGN010000000
VERSION     DAOSGN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79995_length_1595_cov_2.405195"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PK087_03640"
     CDS             <1..435
                     /locus_tag="PK087_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOH18398.1"
                     /translation="AKLAGADYVGDTEYLEKIKAGWFEFDVIVATPDMMGELGKLGKI
                     LGPKGLMPNAKTGTVTMDIDKAVREIKSGKITYRVDKTGNIQVIVGKVSFETEKLADN
                     IRVVYQTLIRLKPSTVKGIYVKNIAISTTMGPGVKILPDSIA"
     misc_feature    456..574
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            588..1076
                     /gene="rplJ"
                     /locus_tag="PK087_03645"
     CDS             588..1076
                     /gene="rplJ"
                     /locus_tag="PK087_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003140120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOH18399.1"
                     /translation="MKEATLQKKVEEVEILTKKFHDAKSIVFVDFLGLTVAEVSELRK
                     SLYAQGCHLYVTKNNILRRAAKQAGYQGLDDILVGPSAVALSHDATAAAKIIFSFAKN
                     NDKLGVKVGVVEGKVMNTADLKVVSNLPNKNGMISMLLSVLQAPIRNLGVAIKAVAEQ
                     RA"
     gene            1097..1468
                     /gene="rplL"
                     /locus_tag="PK087_03650"
     CDS             1097..1468
                     /gene="rplL"
                     /locus_tag="PK087_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOH18400.1"
                     /translation="MEKFNVQDFLKALKEMSVLELNELVKAIEEEFGVTAAVAVAAAG
                     PAEVVEEEGPSTYNVILASFGQNKIAVIKVVRELTGLPLKEAKDLVDAAPKAIKEGVS
                     VDEANAIKSKLEEAGATVELK"
BASE COUNT          532 a          237 c          358 g          468 t
ORIGIN      
        1 gccaagttag ccggagctga ttatgttggt gacaccgaat atcttgagaa aattaaagcc
       61 ggatggtttg aatttgatgt cattgtcgct accccggata tgatgggaga actgggaaaa
      121 cttggcaaaa tcttaggacc aaaaggtttg atgccaaatg cgaaaaccgg taccgtcaca
      181 atggacatcg ataaagctgt tcgggaaatt aaatcaggaa aaattactta tcgcgttgat
      241 aaaaccggca atatccaagt gattgttggc aaagtaagct ttgaaacgga aaagttagct
      301 gataacattc gtgttgttta tcaaactcta attcgtttaa aaccgtcgac cgtcaagggg
      361 atttatgtaa aaaacatcgc gatatcaaca accatggggc ctggagttaa aattttacca
      421 gactcaatcg cttaacaaaa aaaacacaaa caataaaata gagtgagtta ttgccgtaga
      481 cagtaggtgc gtaagcttaa tttcctaccg aggtttaaag atgtttttgt gacctctttt
      541 tccttgatgg taaaagaggt ttattatttt tcaatggagg ttaaataatg aaagaagcaa
      601 cattgcaaaa gaaagtcgaa gaagttgaaa tcttaacgaa gaaattccat gatgccaaat
      661 caatcgtctt tgttgatttt ttaggcttaa ccgttgcgga agtatccgaa cttcgcaaat
      721 ccttatacgc tcaaggttgt catctatatg ttactaagaa taacatttta cggcgggcag
      781 ctaaacaagc cggttatcaa ggcttggatg atattttagt gggaccaagc gccgttgctt
      841 tgtcgcatga tgcaaccgct gcagcgaaaa taatttttag ttttgctaaa aataatgata
      901 aattgggcgt taaagttggt gtggttgaag gtaaagtcat gaacactgct gatttaaagg
      961 tggtatcaaa tttaccgaac aaaaatggta tgatttcaat gttactcagt gtattacaag
     1021 ccccaattag aaatttgggc gtagctatta aagctgtagc tgaacaaaga gcttaatttt
     1081 taaggaggaa tttaaaatgg aaaaatttaa tgtacaagat tttttaaagg cattaaaaga
     1141 aatgtcggta ttggaattga atgaattagt gaaagctatt gaagaggaat ttggcgtaac
     1201 tgcggctgtt gctgttgctg cggctgggcc agctgaagtc gttgaagagg aaggaccatc
     1261 aacttataat gttattttag cttcgtttgg ccaaaacaaa attgcggtca ttaaagttgt
     1321 tcgtgagtta acgggacttc cacttaagga agctaaagat cttgttgatg ctgctcctaa
     1381 agcgattaaa gaaggcgttt ccgttgatga agctaacgct attaagtcga aattggaaga
     1441 agcaggcgct actgtggaat taaagtaata tgattaggag tcatcgaagt tgttcgatga
     1501 ctttttaatt gcgagcgaag gggaattacc taaaaaagca attgactatt tagacagaag
     1561 aggttataat aaattgtaat tttttcaaaa tattt
//
LOCUS       DAOSGN010000234         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_80136_length_1593_cov_2.880364, whole genome shotgun sequence.
ACCESSION   DAOSGN010000234 DAOSGN010000000
VERSION     DAOSGN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80136_length_1593_cov_2.880364"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..>1593
                     /gene="thrS"
                     /locus_tag="PK087_03655"
     CDS             11..>1593
                     /gene="thrS"
                     /locus_tag="PK087_03655"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006059205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOH18401.1"
                     /translation="MNIKFLDGRVKQFTPGQSALDIANSLAVSLAKKAVFAKVNGVDF
                     DLTRPIFEDATLELMTKDSPEALEVLNHSCSHLLASAVRKLYPQAQFGVGPAIEEGFY
                     YDINPGEGIKLQEEDFVKIENEMRQIAKQDLAFKRMELTKAEALKLFSHDKYKQEIIN
                     ELDDHVLITVYQHGDFVDLCRGPHVVSTKVLKNVKLLALSGAYWRGDSQNEQLQRIYG
                     TCFFTEEDLAKHLLVLKERSERDHRKLGRELDLFMLSEYGPGFPFWLPKGMILRKNLE
                     DFWYKIHTRENYQFIQTPIMLNKELWEISGHWFNYRENMYTSEIDKHQFAIKPMNCPG
                     GMLVYKNSIHSYRDFPLRVGELGLVHRHEASGALNGLFRVRNFTQDHAHIFMTEQQIV
                     YEVGNLIKLFEEIYAVFGLSYHIELSTRPKEKYIGSIEIWDRSEQALALACESVGKPF
                     KINPGDGAFYGPKLDFKLRDSMHRIWQCGTIQLDMNLPERFDLTYIDENGERKRPVML
                     HRAIFGSLERFIGIITEHYG"
BASE COUNT          461 a          287 c          360 g          485 t
ORIGIN      
        1 gagaaacaag atgaacatta agtttttaga tggccgtgtt aaacaattta ccccggggca
       61 atcagccttg gatatcgcca attctttagc ggtttccttg gcgaagaaag cggtgtttgc
      121 gaaggtcaat ggggtggatt ttgatttaac acgcccgatt tttgaagatg ccaccttgga
      181 actgatgact aaggattccc cggaagctct ggaagttctt aatcattcct gcagccattt
      241 gttggccagt gccgttcgca aactctatcc gcaagcgcag tttggggttg gtcccgccat
      301 tgaagagggt ttttattatg acattaaccc cggtgaagga atcaaacttc aagaagaaga
      361 ttttgttaaa attgaaaacg aaatgcgcca aatagctaag caagacttag cttttaaacg
      421 gatggaatta acgaaagccg aggctttaaa actgttttca catgataaat acaaacaaga
      481 aattattaac gaactggatg atcatgtcct tatcaccgtt taccaacatg gtgattttgt
      541 cgatttgtgt cgtggtcccc atgttgttag cactaaagta ctaaaaaatg tcaaactttt
      601 ggcccttagc ggtgcctatt ggcgtggtga ttcccaaaac gaacaattac aacgaattta
      661 tggcacttgc ttctttacgg aagaagactt ggctaagcat ttattggttt taaaagaacg
      721 gagtgaacgg gatcatcgaa aactgggacg ggaattggac ttgttcatgt tgtcggaata
      781 tggtccgggc tttccgtttt ggttacccaa ggggatgatt cttcgtaaaa atttagaaga
      841 tttctggtac aaaattcata ctcgtgaaaa ctaccaattt attcaaacac ccattatgtt
      901 aaataaggaa ttatgggaaa tctccggcca ttggtttaat tatcgggaga atatgtatac
      961 ttccgaaatc gacaaacatc aatttgctat taagccgatg aattgtccgg gtggaatgtt
     1021 agtttataaa aacagtatcc attcatatcg agattttccc ctgcgcgttg gggaactagg
     1081 tttagttcat cgtcatgaag cgagcggggc tttaaacggt ttattccggg ttcgtaattt
     1141 cactcaggat catgcccata tttttatgac tgaacaacaa attgtttacg aggtcggcaa
     1201 tttgattaaa ttgtttgaag aaatttatgc cgtgttcggt ttaagttatc atattgaatt
     1261 atcaacccgc ccgaaagaaa aatacatcgg ttcgattgaa atttgggatc gttccgaaca
     1321 ggctttagct ttagcttgcg aatcggttgg taaaccattt aaaatcaatc cgggtgatgg
     1381 cgctttttat ggtccgaagt tggactttaa actgcgtgat tcgatgcacc gcatttggca
     1441 atgtggaacc attcagttag atatgaactt accggaacgc tttgatttaa cttatattga
     1501 tgaaaacggg gaaagaaaac gaccggtgat gttgcatcgc gctatttttg ggtccttgga
     1561 acgctttatc ggcattatta cggaacatta tgg
//
LOCUS       DAOSGN010000235         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_80947_length_1583_cov_2.554319, whole genome shotgun sequence.
ACCESSION   DAOSGN010000235 DAOSGN010000000
VERSION     DAOSGN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80947_length_1583_cov_2.554319"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PK087_03660"
     CDS             complement(<1..524)
                     /locus_tag="PK087_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOH18402.1"
                     /translation="MTKRDYYEVLGVSKTANDDEIKKAYRALAKKYHPDISKDPNATE
                     KFKEVQEAYEILSDPAKREQYNQFGHEGPNMGQGFEGFNFGGGFGGFGGFEDILSSIF
                     GGSRRESGSGRTRGADLRTTITITFEEAAFGVEKEINITKYDTCSTCSGLGAQSKNDI
                     EVCPKCRGRGKVVM"
     gene            complement(550..>1583)
                     /locus_tag="PK087_03665"
     CDS             complement(550..>1583)
                     /locus_tag="PK087_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOH18403.1"
                     /translation="SSLTRAKFDELTAFLVEKTVGPVRQALKDAKMTAKDIDQVLLVG
                     GSTRTPAVQDAVKRELSKEPNKSVNPDEVVAMGAAIQAGVLAGDVKDVLLLDVTPLSL
                     GIETLGGVFTKLIERNTTIPTSKSQIFSTAADNQPAVDIHVLQGERPMAADNKTLGRF
                     QLGDIPLAPRGVPQIEVSFDIDANGIVNVKAKDLGTGKSQSVTIQGGSGLTEAEIKRM
                     VKEAEENAENDRLRKEEADLRNECEQIIFAAKKSVDDLGAEVSEEEKKDIESKSDALK
                     KALEGKDLDDIKAKKEDLLKVSQKVAARAYEKAQKEHQSHEHDQHSHEEQSNAKDEKV
                     VDAEFEDVD"
BASE COUNT          422 a          372 c          262 g          527 t
ORIGIN      
        1 tccataacaa ccttgccacg accacggcat tttggacata cttcaatatc atttttggat
       61 tgggcaccca aaccgctaca tgtcgaacaa gtatcgtatt tagtaatatt aatttctttt
      121 tcaaccccaa aagccgcttc ctcaaaagta atggtaatcg tggtccttaa atccgcacct
      181 ctggttcttc ccgaaccgga ttcacgccga gaaccaccaa agatggaaga caaaatatct
      241 tcaaaaccac caaacccacc aaatccgccc ccaaagttga atccttcaaa gccctgcccc
      301 atattaggac cttcatgacc aaattgatta tattgttcac gtttagctgg atcactgagg
      361 atttcatagg cttcttgaac ctctttaaat ttttcggttg cgttagggtc tttactaatg
      421 tcgggatggt attttttcgc taaagcgcgg taagcttttt ttatttcatc gtcgtttgcc
      481 gttttactga ctcccaacac ttcgtaataa tcacgtttgg tcataaattt ctctttctaa
      541 tgaagaagtt taatcgacat cttcaaattc ggcatcgact actttttcat cttttgcgtt
      601 agattgttcc tcatgggaat gttgatcatg ttcatgagac tgatgttctt tttgcgcttt
      661 ttcataagct cgagctgcca ctttttggct aacctttaat aaatcctctt ttttagcttt
      721 aatatcatca agatccttgc cctctaaagc tttctttaag gcatccgatt tactttcaat
      781 atccttcttt tcttcttcgc taacttctgc acctaaatca tcaacagatt ttttggctgc
      841 aaaaatgatt tgttcacact catttcttaa atcagcttct tctttgcgaa gtcgatcatt
      901 ttcagcattt tcttcagctt ctttgaccat ccgcttaatt tccgcttccg ttaaaccgga
      961 accaccttga atggtaacgg attgactttt tcccgtgcct aaatcttttg ccttgacatt
     1021 aacaatacca ttggcatcaa tatcaaaact tacttcaatt tggggtactc ctcttggagc
     1081 tagagggata tcacctaatt ggaagcgacc aagggtttta ttatccgctg ccatcgggcg
     1141 ttcgccttgt aaaacatgaa tatcaacagc cggttgattg tccgcagccg tcgagaaaat
     1201 ttgtgatttg gaagttggaa tggtcgtgtt tcgctcaatt aacttagtaa atacgccacc
     1261 caaagtttca attcctaatg acaaaggagt gacatcgagt aataaaacat cttttacatc
     1321 accagctaac acaccagctt gaatggcggc tcccattgcg acaacttcat cagggttaac
     1381 actcttatta ggttctttgc ttaattctcg ttttaccgca tcctgaacgg ctggagttct
     1441 agttgaacca ccaacaagta aaacttgatc aatatcttta gcggtcatct tggcatcttt
     1501 caaagcttga cgaacgggac ctacagtttt ttcaactaaa aaagctgtta attcatcgaa
     1561 tttagcacgc gttaacgaag agt
//
LOCUS       DAOSGN010000236         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_81822_length_1572_cov_2.327620, whole genome shotgun sequence.
ACCESSION   DAOSGN010000236 DAOSGN010000000
VERSION     DAOSGN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81822_length_1572_cov_2.327620"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1572
                     /locus_tag="PK087_03670"
     CDS             <1..>1572
                     /locus_tag="PK087_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18404.1"
                     /translation="GKKDPYSGGDFGNGGLLKNNNFICSLDYHFNLGDYFVFDNNNSL
                     IDVDFIKESVFVWTNTALYKFDDHLTIIKKRLFESTIFFGIISAFHTFLGFGIENIAI
                     VNLLDLNKLADIYCEEINEDTQYQQLDSVIKIINGYETAYLDVFDFRSFRFPPVHYDN
                     LPAEGPIKSIFGEVTIKDKTSLPLFNPLVFGTYTWNINLQTSFGLEYVINRQTLVQKE
                     VNVSENNIYPLGYHLHFTGNALLNGQAIVNNYALNESGIYQLTLIGANNEQYSLSFAV
                     AREQIPFEELPERNWHFETRRSGIVQIDLSLQGDWSNLEGVIINGEAFDDFLFDQVNQ
                     RLLVRFKAPDEMGIYHYFIDSIIYRQDNDRLSYPIKYMFTVNVLGFEPEIVIEEINDF
                     EYLIKVNDDDACARSISVHAVSNLDDYVINLPLSDSNIVFPNLNKKQNYELTYFLNYD
                     IGNHQIASIELLSAKMSGKNASDFGEITILEKTNQLKKLTLSFLESKDSITEIIANNH
                     FVYQKPVNNIFSVVL"
BASE COUNT          490 a          253 c          280 g          549 t
ORIGIN      
        1 ttgggaagaa agatccttat tcgggtgggg actttggcaa cggcgggcta ttaaaaaaca
       61 acaattttat ttgttctttg gattaccatt ttaatttagg tgattatttt gtttttgata
      121 ataataattc tttaattgat gttgatttta taaaagaatc cgtctttgtt tggactaaca
      181 ctgctttata caaatttgat gaccatttaa caataataaa gaagcgactt tttgaaagta
      241 ccatcttttt cggcattatc agcgccttcc atacttttct tggtttcgga attgaaaata
      301 ttgctatcgt caatcttctt gatttaaata agcttgcgga tatttattgt gaggaaatca
      361 atgaagatac ccaataccaa caacttgatt ccgtcattaa aatcatcaat ggatatgaaa
      421 ctgcttattt agatgttttt gatttccgtt catttcgttt tcctcctgtt cattatgata
      481 atttaccagc tgaaggtccc attaaaagta tttttgggga agtgacgatt aaggataaaa
      541 cctcacttcc gctctttaat ccgctggttt ttggtactta tacttggaac atcaacttac
      601 aaacaagttt tgggcttgag tatgtaatta atcgacaaac gcttgttcaa aaagaagtca
      661 atgtgagtga aaacaatatt taccccttag gttatcattt gcattttacc gggaatgctc
      721 ttttgaatgg tcaagccatt gttaacaatt atgctctcaa tgaaagcgga atttatcaac
      781 tgacattgat aggggctaat aatgaacaat attcgctttc ctttgctgtt gcaagagagc
      841 aaatcccttt tgaagaactt cctgaacgaa attggcattt tgaaacaaga agaagtggga
      901 ttgtccaaat tgatttgagt ttacaaggag attggagcaa tttggaaggc gtcattatca
      961 atggggaagc ttttgatgat tttttgtttg accaagttaa tcagcgtttg cttgttcgct
     1021 ttaaagctcc tgacgagatg ggtatttatc actattttat tgatagtatc atttatcggc
     1081 aagataatga tcgattgtca tatcccatta agtatatgtt caccgtcaat gtcctagggt
     1141 ttgaaccgga aattgtcatt gaagaaataa acgattttga atatttaatc aaagttaatg
     1201 acgatgatgc ttgtgctcgc tccattagcg ttcatgctgt ttccaattta gatgactatg
     1261 tcatcaattt gccattatcg gacagtaata ttgtgttccc taacttaaac aaaaagcaaa
     1321 attacgaact tacttatttt ttaaattatg atatcgggaa tcatcaaatc gcttcaattg
     1381 aactacttag tgcgaaaatg agcggtaaaa acgccagtga ctttggtgaa atcaccattc
     1441 ttgaaaaaac gaaccaactt aaaaaactaa ccctatcctt tttggaaagt aaagatagta
     1501 ttacggaaat tatcgctaac aatcattttg tttaccaaaa accggtaaac aatatctttt
     1561 ccgtagtgtt gg
//
LOCUS       DAOSGN010000237         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_82357_length_1565_cov_2.410596, whole genome shotgun sequence.
ACCESSION   DAOSGN010000237 DAOSGN010000000
VERSION     DAOSGN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82357_length_1565_cov_2.410596"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK087_03675"
     CDS             complement(<1..258)
                     /locus_tag="PK087_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18405.1"
                     /translation="MDLFKKLHPTVLLIFFTAVLFLTMLTANPLFLVISFVCALLSNA
                     FFQKPKRFLTSFVTIILMIILITITNPLFNRHGATVLFNIGR"
     regulatory      complement(330..507)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(918..1103)
                     /locus_tag="PK087_03680"
                     /pseudo
     CDS             complement(918..1103)
                     /locus_tag="PK087_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          542 a          279 c          247 g          497 t
ORIGIN      
        1 tctaccaata ttgaataaaa ccgtcgcccc gtggcgatta aacaagggat tagttattgt
       61 tataaggatt atcataagaa taatcgtcac aaaggaagtt agaaaccgct ttggtttttg
      121 aaaaaacgca ttactcaaaa gcgcacaaac gaaagaaata acaagaaaaa ggggatttgc
      181 tgtgagcatg gttaaaaaaa ggacagcagt aaaaaagatt aataagaccg tcggatgtaa
      241 ttttttaaat aaatccatac ttcctcccat aaaaaaagcc accaagggtg gcaaataaga
      301 agtccaaata agccatctcc caccgaaatg ttttgatttt atttaatggc aggtctcctg
      361 acttggacat caacacaaac tccaccttcc cggtttccca gtggcatttc gaagtttatt
      421 attccttaca gtagcggggg ctgttacggc tttgaccgta ttccctttta atgcttacat
      481 taagcaacca atgaatatta gtattaattt tttttaaagc cgattctcac aaaatcaata
      541 taattattgt aatcatgttt aattttgtct ttatccgccc gtttggttaa gaaaatctct
      601 aaacaatttt taccaatcat ttttataata cttaacaatt tgggaaaatg ctgggatttt
      661 ttaatttcat ccggaaaata ggtttgatca atatcgacat ccggttccac aatctttggc
      721 aacaatgatt catacaaaga attttcatta aaaaccgttg aaataacatt gaggcggaac
      781 tgccgatttt ctttgttggt tagtttatca atggtatgag aaaacttaat tttaagggct
      841 tcataaacat cgttatccgt ttgttcgaga tattgggtaa acttacgttt atcgatacca
      901 tacaaataat gaagcaaata aacaaataaa tcttccacat tttcaaaata ttggtaaaaa
      961 ctgcctcgag gaatatccgc atccttcaca atattagcga tgatggcccg ttccaaagga
     1021 actcgcgaaa attctttttt ggcagctttc aaaatgcgta gacgtttttc tttaggtaaa
     1081 caaagaaatg ttttagttgg catgtttttc ctccttactc atcgtcatta attccatttt
     1141 gttatgttat tttatcacaa acaaagttaa aagtcaatta atccgattgg ctaaaaacaa
     1201 agcgaaaata gcaattatta tgaggatttt tccaacaatt aagaaattaa acctttttac
     1261 aacgcttgtt tattgataaa tcccgagcaa aaataagata atcgcctgta aacagccctt
     1321 tttaggtctt tttcagtgtt tttaagcgaa aaaatgcctt tttaagctct ttttttccaa
     1381 aaatgaagat ttttatattt ttaaatcacc taattgaatc caacccattt ttcggaatat
     1441 gagatcaatt aaccacacta aaaggatggg aagggcgatt tgtaagacga aaacgcttaa
     1501 ccaaaaccat aattgattac ccatggccga ataagtagca aattgcccaa ccaaaccgga
     1561 agttc
//
LOCUS       DAOSGN010000238         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_82669_length_1561_cov_2.940903, whole genome shotgun sequence.
ACCESSION   DAOSGN010000238 DAOSGN010000000
VERSION     DAOSGN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82669_length_1561_cov_2.940903"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PK087_03685"
     CDS             <1..297
                     /locus_tag="PK087_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18406.1"
                     /translation="SIVVFFILTPEIIGITYGAGVILMSYKQQEMNKKDVLLVSVFLC
                     LCHAIVEDTLLFAAFGSITWVVIVVKLVVAFTTTMLVNLILKLIEHRQLAKNPD"
     gene            366..>1561
                     /locus_tag="PK087_03690"
     CDS             366..>1561
                     /locus_tag="PK087_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH18407.1"
                     /translation="MAKKVIIIGGVAGGATTAARLRRLDEQVEIIVFERGEYISFANC
                     GLPYYIGGVIANREALILQTPEELRQKYRLDIRVQSEVESINPKKKTVMVRNLVSQEQ
                     YEESYDDLVISTGAFPVKPPIPGIDETTNLFTLRNIPDTDQIKDFINQKEPQTALVIG
                     GGFIGLEMAENLHHLGMKVTIVEMADQVMAPIDLEMAMIVHEHIISQGVNLILKDGVK
                     AFIDHGQKVELTSGKVVEADLIILCIGVRPENKLAKQAGLALGARGGILVNESLQTLD
                     PHIYAIGDVIEVTDFVSRQPAMIPLAGPANKQARIVANNICGIKEQYGGTMGTSVAKV
                     FALTVAATGNNEKLLKSQGKKYDAIHIHPFSHATYYPNATPIDLKLLYNPETEEIYGA
                     QAIGIKG"
BASE COUNT          475 a          286 c          350 g          450 t
ORIGIN      
        1 agcattgttg tatttttcat tctgactcct gaaattatcg gcattactta tggcgccgga
       61 gttatcttaa tgagttacaa acaacaagaa atgaacaaga aagatgtctt attagttagt
      121 gtttttcttt gcttatgcca tgctattgta gaagatactt tgttgtttgc tgcttttggt
      181 tcgattactt gggtggtgat tgttgtaaaa ttagtggtgg cgtttaccac aaccatgctt
      241 gtcaacctga ttttgaaatt aatcgagcat cgtcaattgg caaaaaaccc tgattaaaag
      301 gtcacttcga cctcttcaaa tgtttgaaaa tatggtataa ttaaaagcag aaaaaggaga
      361 tcaatatggc taaaaaagta attattatcg gtggtgtagc aggaggagcg acaactgccg
      421 cccgactgcg ccgcttggat gaacaagttg aaatcattgt ttttgaacgg ggcgaatata
      481 tttcgtttgc taactgtggg ctcccttact atattggggg cgttattgcc aaccgtgaag
      541 ccctcatctt acaaacaccg gaagaactgc gccaaaaata ccgcttagat attcgcgttc
      601 aatcggaagt tgaaagtatc aatcctaaaa agaaaaccgt catggtaaga aatttagtaa
      661 gtcaagagca gtacgaagag agctatgacg atttggttat ttcgactggg gcttttccag
      721 ttaaaccacc tattcctggc attgatgaaa ccactaactt atttacactg cgtaatattc
      781 cggatacgga tcagattaaa gatttcatta atcaaaaaga accccaaacc gctttagtca
      841 ttggcggtgg gttcattggc ttggaaatgg cggaaaatct ccaccattta ggcatgaagg
      901 tgacgattgt ggaaatggcg gaccaggtta tggcccccat tgatttggaa atggcaatga
      961 ttgttcatga acatattatc agccaaggcg tgaacttgat tttaaaagac ggcgttaaag
     1021 cttttattga ccatggccaa aaagtggaat taacgagcgg caaggtagtc gaagcggatt
     1081 tgattattct ttgcattggt gttcgtcctg aaaataagct tgcaaaacaa gcgggcttag
     1141 ctttgggagc acggggagga attttggtca atgaaagttt acaaaccctt gacccacata
     1201 tttatgcaat tggcgatgtt attgaagtta cggattttgt ttcccgtcaa ccagcgatga
     1261 ttcccttagc cgggcctgct aacaaacaag cccggattgt tgccaacaat atctgtggga
     1321 ttaaggaaca atatggagga accatgggaa catcggtagc aaaagtcttt gctttaactg
     1381 ttgccgcaac ggggaataat gaaaaattat tgaagagtca aggaaaaaaa tatgatgcca
     1441 ttcacatcca tcctttttct catgccactt attatccgaa tgcgacgccg attgatttaa
     1501 aattattata caatccggaa acggaagaaa tctatggtgc ccaagccatc ggcatcaagg
     1561 g
//
LOCUS       DAOSGN010000239         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_83919_length_1547_cov_2.329759, whole genome shotgun sequence.
ACCESSION   DAOSGN010000239 DAOSGN010000000
VERSION     DAOSGN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83919_length_1547_cov_2.329759"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PK087_03695"
     CDS             complement(<1..331)
                     /locus_tag="PK087_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOH18408.1"
                     /translation="MKMKYLFTSESVTEGHPDKVCDQIADAILDAILKDDAHAKVACE
                     AVATTGLVLVMGEITTTTYVDIQKIVRETVAAIGYDRGKIGFDAENLAVLVCVNEQSP
                     DIANAVRD"
     gene            complement(441..>1547)
                     /locus_tag="PK087_03700"
     CDS             complement(441..>1547)
                     /locus_tag="PK087_03700"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOH18409.1"
                     /translation="TTLKNNALSLTSDEEFVIFGGSDSYPQVQAIDSGFEKVTIDKKV
                     EMVLSLEKHIKALDQRIVFVPYCGYSEVKQSITIINSLGLNLTKANEYAYLVAQAVAK
                     ENDDTQSCFDVEVKLRYDDLHVETLAKKVVDKTLAKLNASPVASKNYPIIFEREAMGD
                     LFNTFDSFFSGEAAIKKLTPLLNKEGEKIMSDKITIVDDPLHRDAINLQPFDDEGVAC
                     YRKTVVEKGVFKTLLHNLKTAKYFKTTSTGNGFKPSVAATVNVSGTNLLIEPGEKTLA
                     QLVSETQEGLLITELTELHAGANPITGDFSAQSSGFLIKDGKIDRPVNLIVVSGNFLK
                     MMNDVESVGSDLKLGYNGIGTPSIKFVGLQVSGK"
BASE COUNT          463 a          343 c          257 g          484 t
ORIGIN      
        1 catcccgaac agcattggca atatcgggag attgttcgtt aacacatact aaaacagcta
       61 aattttccgc atcaaagcca atcttcccac gatcataacc gatggcagcc acggtttccc
      121 gcacaatctt ttgaatatcg acataagttg tggtggtgat ttcccccata acaaggacta
      181 aacccgttgt tgcaacagct tcacaagcca ctttcgcatg agcatcatct tttaaaatcg
      241 catctaaaat ggcatcagca atttggtcac aaaccttatc gggatggcct tcagtaacag
      301 attctgaagt aaataaatat ttcattttca ctctccttct tttttggttt tcgctttctt
      361 taaataaaag caccaatctt tacatattat tgaaaattat cttctaaaaa aatacctctt
      421 aaaaaagaga tatttataaa ttatttaccg gaaacttgca gaccaacaaa tttgatcgat
      481 ggtgttccaa tcccattata tcctaactta agatcactgc cgacgctctc gacatcattc
      541 atcattttta agaaatttcc ggaaacaaca attaagttaa ccggacggtc aattttgccg
      601 tctttgatga gaaaacccga agattgagca ctaaaatcac cggtaatcgg attagctccg
      661 gcatggagtt cggtcaattc ggtaattaac aagccttctt gggtttccga aaccaattgt
      721 gctaaagttt tttcccccgg ttcaatcaag agattggtgc cactgacatt aacagtcgcc
      781 gccaccgagg gtttaaaacc attgccggtg gaagtcgttt taaagtattt ggcggtcttt
      841 aaattatgca ataaggtctt aaagacccct ttttccacaa ccgtcttgcg ataacaagca
      901 acaccttcat catcaaaagg ctgtaagtta atggcatcac gatggagtgg gtcatcaaca
      961 atcgtgattt tatcactcat tattttttcg ccttctttat ttaataaagg ggttaatttc
     1021 ttaatcgctg cttctcccga aaaaaagctg tcaaaagtgt taaacaagtc tcccatggcc
     1081 tctctctcaa aaataatggg gtagttcttt gaagcaaccg ggctcgcatt taattttgct
     1141 aaagtcttgt ccacaacttt tttcgccagc gtttccacat gcaaatcatc atatcgcagt
     1201 ttcacttcaa catcaaagca ggactgtgta tcatcatttt ctttcgccac cgcttgcgca
     1261 actaaataag catattcgtt ggcctttgtt aagtttaagc ctaaagaatt aataatcgta
     1321 atgctttgtt taacttccga atacccacag tacggaacaa aaacaattct ttggtcaagt
     1381 gctttaatgt gcttttccaa ggataagacc atttccactt ttttatcaat cgtgactttt
     1441 tcaaaaccac tgtcaatggc ttgcacttgt gggtaggaat cgctaccacc aaaaataaca
     1501 aattcttcat cgcttgttaa ggacagggca ttgtttttta aagtggt
//
LOCUS       DAOSGN010000240         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_84738_length_1538_cov_2.015509, whole genome shotgun sequence.
ACCESSION   DAOSGN010000240 DAOSGN010000000
VERSION     DAOSGN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84738_length_1538_cov_2.015509"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1370
                     /locus_tag="PK087_03705"
     CDS             60..1370
                     /locus_tag="PK087_03705"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOH18410.1"
                     /translation="MLRLRAVDLIIKKKQGLALSCEEIHFLIQGYLSGAIPDYQMSSL
                     LMAICFQGLNETEQLALTLEMLKSGEEVDLSSIKGICVDKHSTGGVGDKTTLIVAPIV
                     ASCGLKMAKMSGRGLGHTGGTIDKLEAIPGFKVDVSPENFLKQVSEIGLAVVGQTANI
                     APADKKLYALRDVTGTIEAVGLIASSVMSKKLASGASHILLDVKVGNGAFMKTVEEAR
                     TLAQAMVKIGNRAHRHTVALLTNMDQPLGFAVGNALEVIEAMETLRGRGPQDLTKLCY
                     ELAAELLLMTNMVVTKEEGLKQVEHAITSQAALDSFRQMIKYQQGDTKVLEDYSLLAK
                     SKYIFEVKARKDGYISQVKALAIGQAAMLLGAGRAKKDDVIAMGVGVKAFGKVGDYVH
                     QGDVLATIHANEEKIDEVFTMVLEAFVISPHQVLAKSIILEIVS"
BASE COUNT          424 a          260 c          404 g          450 t
ORIGIN      
        1 tatctaaata atttggcttt attttaggaa ttatggtata atattatcta ccaaaagggg
       61 tgttacgatt gcgtgcggtt gatttaatta ttaaaaagaa acaaggactg gctttgagtt
      121 gtgaagaaat ccatttttta attcagggtt atttatccgg ggcaattcct gattaccaaa
      181 tgtcgagttt attgatggcc atttgctttc aaggcttgaa tgaaactgaa caattagcct
      241 tgactttgga aatgcttaag agtggtgaag aagttgattt atcatcaatc aaaggtattt
      301 gtgtggataa acattcgact gggggtgttg gtgataaaac gacgctgatt gtggcgccca
      361 ttgttgccag ttgcggcttg aagatggcga aaatgagcgg tcgggggcta gggcacactg
      421 ggggaacgat tgacaaatta gaggccattc ccggctttaa ggtggatgta tcaccggaaa
      481 actttttaaa acaagtttcg gaaattggtt tagcggtggt tggccaaact gctaacattg
      541 cgccggcgga taaaaaactc tatgctctcc gtgatgtgac ggggacgatt gaagctgtcg
      601 gcttaattgc cagcagtgtg atgtcgaaaa aactggctag tggcgcttcc catattttgt
      661 tggatgtgaa agtcggcaac ggagcattta tgaaaactgt ggaggaagcg cgtactttag
      721 cgcaagcgat ggttaagatt ggtaatcgtg ctcatcggca caccgtcgct cttttaacca
      781 atatggatca accgctcggt tttgcggtcg gtaatgcttt ggaagtcatt gaagccatgg
      841 aaacattaag gggccgagga ccgcaggatt taaccaaact ctgttatgaa ctggccgcgg
      901 aactcctttt aatgacgaat atggtggtaa cgaaagaaga aggcttgaaa caagttgagc
      961 acgccattac ttcccaagcc gccttggatt ctttccgtca aatgattaag tatcaacaag
     1021 gagataccaa agtacttgaa gattattctt tgttggccaa gagtaagtat atttttgaag
     1081 tcaaagcgcg caaggatggt tatatttccc aagtcaaagc tttggccatt gggcaagcgg
     1141 cgatgctctt aggagcggga cgagccaaga aagatgatgt tattgccatg ggcgtcggtg
     1201 ttaaagcctt tggtaaagtt ggtgattatg ttcaccaagg tgatgttctg gcgaccattc
     1261 acgctaatga ggaaaagatt gatgaagttt tcacaatggt tttggaagcc tttgtgattt
     1321 cgcctcatca agtgctagca aaatcgatta tattggagat tgtctcttag aaaggaaggt
     1381 gaaattatgg ctttaatatg ggaacaaatt ttgcgagtca ttgcggcggc tttagtgggg
     1441 gctggcattg gctacgaacg ggaagttaaa aataaaccgg ccggcttttt tacctttact
     1501 ttagttagtg tcggtagttg cctcattgcc attttaca
//
LOCUS       DAOSGN010000241         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_84769_length_1537_cov_3.337382, whole genome shotgun sequence.
ACCESSION   DAOSGN010000241 DAOSGN010000000
VERSION     DAOSGN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84769_length_1537_cov_3.337382"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="PK087_03710"
     CDS             complement(<1..1501)
                     /locus_tag="PK087_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="MutS2; MutS-II; involved in blocking homologous and
                     homeologous recombination; has ATPase activity stimulated
                     by recombination intermediates; inhibits DNA strand
                     exchange; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOH18411.1"
                     /translation="MSHSIYCPLFEDYIKQLFYPKELNLRLKEIINPYGEILDSASEN
                     LRKIRKSIKDCERSIQNKLQELLAKNVQKLSQATISMRNDRYVLPVKADQKGGFPGIV
                     HDQSASKETVFIEPLAVLEINNRLNALKEEEKNEIAFIIRKVAFEIDNYYEELLNNFS
                     IFVNLDLIFAKAQYALNINAKKPKINNQAYVDLINCRHPLLNVEKVVPNNIAIGKNYQ
                     GIIITGPNTGGKTVVLKTIGLLSLMVKMGMLIPCDEDSDIMIFDNVFADIGDEQSISQ
                     NLSTFSSHMRNVIDIIHRVTNHSLVLLDELGSGTDPVEGSSLAIAIFDYLISKDCLVI
                     ATSHYSELKIHAYDSDHIINASVEFDSQSLMPTYKLLLGIPGQSNALNISKHLGLPEE
                     ILSQSEKYVGAKDTDLNKIIAKLTQQTSELDQKLALAEEKNQVLMDKIKSTDQLQKTL
                     FDEKNRLLKSAEEEARKIIVRTTKKMDMILAELEQMKLKEVKLHEIADIK"
BASE COUNT          456 a          260 c          279 g          542 t
ORIGIN      
        1 gtttgatgtc ggcaatttca tgaagtttaa cttcttttaa tttcatttgc tcgagttctg
       61 caagaatcat atccattttc ttggttgttc gaacaatgat tttacgagct tcttcttcgg
      121 cactttttag aaggcggttt ttttcatcaa aaagggtttt ttgtagttga tccgtgcttt
      181 taattttgtc cattaagact tggttttttt cttcagccag agctaatttt tgatcgagct
      241 cgcttgtttg ctgcgtcaat ttcgcaataa tcttatttaa atccgtgtcc ttggctccaa
      301 catatttttc tgattgggaa agaatttcct caggtaaacc taaatgcttg gaaatattaa
      361 gggcattact ttgacctgga attcctaaaa gcaatttata agttggcatt aagctttggc
      421 tatcaaattc gacgctggca ttaataatat gatcggaatc gtaagcgtga atttttagtt
      481 ccgaatagtg ggaagtagca attactaagc agtctttact aattaaataa tcaaagatgg
      541 caatggctaa actcgaaccc tcaacagggt cagttcccga ccctaactca tcaagtagga
      601 ctaatgaatg attggtaaca cgatgaataa tgtcaatgac attgcgcata tgggatgaga
      661 aagtcgatag gttttgcgag atactttgtt catcaccaat gtcggcaaaa acattatcaa
      721 aaatcataat gtcactgtct tcatcacagg ggattaacat ccccattttc accattaacg
      781 aaagaaggcc gatggtttta aggacaacgg tttttcctcc tgtgttcggt ccggtgataa
      841 taatcccttg ataattttta ccaatggcaa tgttgttagg aacgactttt tcaacattca
      901 ataatggatg acggcaattt attaaatcaa cataagcttg attatttatt ttcggctttt
      961 tggcattaat atttaaagca tattgtgctt tcgcaaaaat taaatccaaa ttaacaaaga
     1021 tggaaaaatt gttaagtaat tcttcatagt aattatcgat ttcaaaagca acctttctaa
     1081 ttatgaaagc aatttcgttt ttttcctctt cttttaaagc atttaaacga ttattgattt
     1141 ctaagactgc taaaggttca ataaaaactg tttcttttga ggccgattga tcgtgaacaa
     1201 ttccgggaaa accccctttt tggtcggctt tgaccggtaa gacataacgg tcattacgca
     1261 tggaaatcgt tgcttgcgaa agcttttgga catttttagc taataattct tggagtttat
     1321 tttgaatgga gcgctcacaa tccttgatac ttttcctgat ttttcgtaaa ttttccgaag
     1381 cggaatcgag gatttcgcca tagggattaa taatttcttt taggcgaaga tttagctctt
     1441 tgggataaaa tagttgtttt atataatcct caaacaaagg gcaatagata ctatgactca
     1501 tcaatgtttc caaaaagatt aaattggctt tggtaga
//
LOCUS       DAOSGN010000242         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_85052_length_1534_cov_2.350237, whole genome shotgun sequence.
ACCESSION   DAOSGN010000242 DAOSGN010000000
VERSION     DAOSGN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85052_length_1534_cov_2.350237"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1534
                     /locus_tag="PK087_03715"
     CDS             39..>1534
                     /locus_tag="PK087_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOH18412.1"
                     /translation="MKELLEKLFTKENYQPLTVGEIFEELKLSPDDWEELHLTLAQMV
                     ENHDLFLSKKKDRFLIPQQVGIFKGTISIKHEDFGFIISEGLDDVYVSHFNFGNAIDR
                     DEVLFQIKDYGKFPHQDFRQEAKVLKIIKRHLTDVVGELKWKKEQFFIYVNHPQIARL
                     NIVDNEILSLCQVGDIVRVHITDYLSSPKQARIVERLGFKNDIGIDIIQIASNFNFSL
                     EFTKATEAYLTTINQDFSKEIAKRPLPSLERIITIDGEDAKDLDDAVAVKKLANGNFL
                     LGVYIADVSYFVAKDSPLDEEALFRGTSVYLVNRVIPMLPPLLSNNLCSLNPGQSKLV
                     LACEMEINDQGQVVNQTLFPSVIKTVHRMTYTNVNLMLDYDKETLSNYQDIAADIMVM
                     KELATVLNKMRVKRGALDFNIEEGKIIVDDQGKPLRVELESRGQSERIIEEFMLIANE
                     TVASTIYHLDLPFIYRVHEKPNVQKLEAFKKLSRSLGYQAFKKQINAK"
BASE COUNT          496 a          257 c          314 g          467 t
ORIGIN      
        1 ttacaggtct ttttaaatgc caattttatg agggaagaat gaaagaatta ttagaaaaat
       61 tatttaccaa ggaaaattat cagcccttaa cggtgggcga aatttttgaa gaattaaaat
      121 taagccctga tgattgggaa gaactgcatt taactttagc gcagatggtg gaaaatcacg
      181 atttgttttt atcaaagaaa aaggatcgtt ttttaatacc gcagcaagtg gggattttta
      241 aagggaccat cagcatcaaa cacgaagact ttggttttat cattagtgaa ggacttgatg
      301 atgtttatgt ctctcatttc aattttggaa acgccattga tcgtgatgaa gtcttgtttc
      361 aaatcaaaga ttatgggaaa tttccccatc aagattttcg ccaagaagcc aaggttttaa
      421 agattatcaa acgccattta accgatgtcg tcggtgaatt gaaatggaaa aaggaacagt
      481 tttttatcta tgtcaatcat ccgcaaatcg caagattaaa tattgttgat aatgagattt
      541 taagcctctg tcaagtaggc gatattgttc gtgttcacat tacggattat ctaagcagcc
      601 ccaaacaagc aaggattgtg gaaaggctcg ggtttaaaaa tgatatcggc attgatatta
      661 tccaaattgc cagcaacttt aatttttctc ttgaatttac caaagcgaca gaagcttatt
      721 taacgactat caaccaagat ttttctaagg aaattgctaa gcggccgtta cctagtttgg
      781 aacgcattat tacgattgac ggtgaagatg ccaaagattt ggatgatgcc gtagcggtga
      841 agaaattagc caacgggaat ttccttttgg gcgtttatat cgctgatgtc agttattttg
      901 ttgccaagga ttctcctttg gatgaagagg ctttatttag aggcaccagt gtttatttag
      961 tcaatcgggt tattccgatg ttaccacccc tcttaagcaa caatctttgt tccctcaacc
     1021 ccggtcagtc gaagttggtt ttagcctgtg aaatggaaat taacgaccaa ggtcaagttg
     1081 ttaatcaaac tttgtttcca tcagttatta aaacagttca tcggatgact tataccaatg
     1141 tcaatttaat gcttgattat gacaaagaga cattaagcaa ttatcaggat attgccgctg
     1201 atattatggt aatgaaggaa ctggcgacgg ttttaaataa aatgcgcgtt aaacgagggg
     1261 cgctggattt taatattgaa gaaggaaaaa tcattgttga tgaccaagga aaacccctaa
     1321 gagtagaatt ggaaagtcgc ggtcaaagtg aacgcatcat cgaagaattc atgctcattg
     1381 ccaatgaaac ggtggcatca acgatttatc atctcgactt accctttatt taccgggttc
     1441 atgagaaacc taatgtgcaa aagcttgaag cttttaaaaa actgtcccga agtcttggtt
     1501 atcaagcttt caaaaaacaa atcaatgcga aaca
//
LOCUS       DAOSGN010000243         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_85195_length_1532_cov_3.519296, whole genome shotgun sequence.
ACCESSION   DAOSGN010000243 DAOSGN010000000
VERSION     DAOSGN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85195_length_1532_cov_3.519296"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK087_03720"
     CDS             <1..396
                     /locus_tag="PK087_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18413.1"
                     /translation="LGFALFWTIAAMQTALEMAFFGIPFIVVGFFLVFGKKTWMKRIS
                     GHVYYGVTNKRILILTRGQQIAFSEIALESLSNVVLSPGIKGTSSITFYVRNESIFNH
                     KQPFFYSIENGPDVVNLINELRQKRLSSQ"
     gene            complement(450..707)
                     /locus_tag="PK087_03725"
     CDS             complement(450..707)
                     /locus_tag="PK087_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HOH18414.1"
                     /translation="MVICFGVSWPLSIYKSYRSKTAKGKSIIFLCFILLGYALGIANK
                     IVNHDINYVIGFYILNFVMVFIDFLLYFRNKQFDNKKEEIR"
     gene            complement(1040..>1532)
                     /locus_tag="PK087_03730"
     CDS             complement(1040..>1532)
                     /locus_tag="PK087_03730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOH18415.1"
                     /translation="NKIDGEINPEPWIGFFKKEKKDTWKVSGLSLAIIITLVTAIVIY
                     FQVYKNGVIQTFTIGNFLIVVLFALINSFVEEITFRHTFVSIVEYHNLNRYISQGLAA
                     LIFGVGHYFGHPGKIPGVLLAGFLGWLLSKSIHETKGFFWAWLIHFLQDIVIMTALFL
                     TLS"
BASE COUNT          515 a          273 c          233 g          511 t
ORIGIN      
        1 ttaggctttg ccctcttctg gacaattgcg gcaatgcaaa ctgccttaga gatggccttt
       61 tttggaatcc cgttcattgt tgttggcttc tttttagtgt ttgggaaaaa aacatggatg
      121 aagcgtattt ccggtcatgt ctattatggc gttaccaata aacgcatttt aattcttacc
      181 cgcggccaac aaattgcttt ttcagaaata gccttggaaa gtctaagcaa cgttgtgctt
      241 tcaccgggta tcaaaggaac cagttctatt accttttatg tccgcaatga atctattttt
      301 aatcacaaac agccgttctt ctattcaata gaaaacggtc ccgatgtcgt taacctaatt
      361 aatgaactac gccaaaaacg actatcatca caataagaat tttatctttc ggactgttgc
      421 tgcaaagaat ccattgacat aagcaaaaac tatcttattt cttccttttt attatcaaat
      481 tgtttgttgc gaaagtataa taagaaatca ataaagacca tgacaaaatt taggatgtaa
      541 aaaccgataa catagttaat gtcgtgattg acaattttat tggcgatgcc taatgcataa
      601 ccaagcaaga taaaacaaag gaaaataata ctttttccct ttgccgtttt tgaccgatac
      661 gatttataaa ttgatagggg ccaagatact ccaaaacaaa taaccattgc tgcttctaac
      721 ataattattt ctctccttca ttcctaaatt attataactt gtttttattt tatttcaata
      781 acaaagcaaa aaagatatcg tgttgaactg atatcttttt taataagtaa gacgattata
      841 ttaaaagtaa atcctatcgt ttaagttaag atatgaaatt tcctcagcaa tttgaaattg
      901 accacctatt aggtttaacc attcctttgt tatcttaaac cttcgaaata taaaattgta
      961 tacaaagttg attcagtgat tttatatctt taatggtgcg ataaaaaaaa gggcttttgc
     1021 cctctttgtt cttttgatat taacttaaag ttaaaaataa tgccgtcata ataacaatat
     1081 cttgaaggaa gtgaattaac caagcccaga aaaacccttt tgtttcatgg atgctttttg
     1141 ataaaagcca acctagaaag ccagctaata atactcccgg aatttttcca ggatgaccaa
     1201 agtagtgccc tactccaaag attaaagctg ctaatccctg agaaatataa cggtttaagt
     1261 tgtggtattc aacgatactt acaaaagtat gacggaaggt tatctcttca acgaaactat
     1321 taattaaagc aaataacacg acaatcaaaa agttaccgat tgtaaatgtt tgaataacac
     1381 catttttata gacttggaag tagatgacta ttgccgtaac taaagtaata atgatggcta
     1441 atgaaagtcc acttactttc caagtatcct tcttttcttt tttaaagaaa ccaatccacg
     1501 gttcgggatt gatttcccca tcgattttgt tt
//
LOCUS       DAOSGN010000244         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_85309_length_1531_cov_2.968157, whole genome shotgun sequence.
ACCESSION   DAOSGN010000244 DAOSGN010000000
VERSION     DAOSGN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85309_length_1531_cov_2.968157"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /gene="topA"
                     /locus_tag="PK087_03735"
     CDS             complement(<1..1103)
                     /gene="topA"
                     /locus_tag="PK087_03735"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOH18416.1"
                     /translation="MDKNLVIVESPAKSQTIAKYLGENFRVVSSKGHIRDLVTRGYGG
                     FGVDIENNFKPMYSTISGKAPLIKELKKQVKEASKVYLATDPDREGEAISWHLYDVLD
                     LQNKAYERVVFHEITKNAVIKALENGREIDMDLVHSQESRRILDRIIGFSLSKLLQKK
                     IGSRSAGRVQSVALKLICDREKEIQAFVPEEYWEVYLEFIKDNKQWKAKLTSDQNGKI
                     SLVNEQETNHVLAKLKSDFIVSEILTKNRQKAPKPPFITSTLQQEAANLLNFPAKKTM
                     MIAQKLYEGLELAHERVGLITYMRTDSFRLSQEFVLEAQDFIIKQYGKDYFRGHFVGK
                     TKNKNIQNAHEAIRPSSLKYHPDEIKNYLTNDE"
BASE COUNT          463 a          302 c          249 g          517 t
ORIGIN      
        1 aattcatcat tcgttaaata attcttaatc tcatcaggat gatatttcaa tgatgacgga
       61 cgaatggctt cgtgagcatt ttgaatattc ttgtttttcg tcttgcctac aaaatgaccc
      121 cgaaaatagt cttttccata ttgtttaatg ataaaatctt gggcttctaa aacaaattcc
      181 tgacttaaac gaaaggaatc agtgcgcata taagtaatca aaccaacccg ctcgtgggcc
      241 aactcaagac cttcatagag tttttgggca atcatcatcg tttttttggc tggaaagttc
      301 aacaaattag ccgcttcctg ttggagtgtt gaggtaataa aaggcggttt tggggctttt
      361 tgacgatttt tagtaaggat ttcggaaaca ataaaatcac tctttaactt ggctaaaaca
      421 tgattcgttt cctgttcatt aacaagggat attttcccgt tttggtcgct ggttaatttc
      481 gctttccatt gtttattatc cttaataaac tctaaataaa cttcccaata ttcctcagga
      541 acaaaagctt gaatttcttt ttcccgatca caaataagct ttaaggctac ggattgaacg
      601 cgaccagcag aacgcgatcc gattttcttt tgtaataact ttgaaagcga aaacccgata
      661 atgcgatcaa gaatgcggcg tgattcttgg gaatgaacta aatccatatc aatttctcga
      721 ccattttcca gggctttaat caccgcattt ttagtgattt catgaaaaac aactcgctca
      781 taagctttgt tttgtaaatc caagacatca tacaaatgcc aagaaatagc ttcgccttcc
      841 cggtcggggt cggttgctaa atagactttg ctagcttctt tgacttgttt tttcaattcc
      901 ttgattaaag gcgcttttcc actaatcgtg ctgtacatcg gtttaaaatt gttttcaata
      961 tccacgccaa aaccgccata accccgtgtc accaaatcac ggatatggcc tttactggaa
     1021 acaaccctaa agttttctcc taaatattta gcaatagttt gtgatttggc aggactttcc
     1081 acaatcacta agtttttatc catgattttc tccttcaatg gcattgatta tagcactgat
     1141 aaaatcgaaa tgtcaaacaa aatcgttttt attcaaaaat ccgttaaaat aacgatgaag
     1201 caccttcttt attagtcagt aaataacatt ctttagatgt aaaagttaat ttacttggtt
     1261 ctatccgccc gcattcgtca tcgcttgcca acttaccata agcaggtaaa caagaaatcc
     1321 gttctcaccc ctctgaataa gggaagcgat taaacaagtt taaggaagtt tttttaaaat
     1381 aacccggata gttaatcgat aattaaattt tttctaaaca agtttccttc cctaacttca
     1441 taaatagttt atttcctttg gttttgaaaa caaaaatcat cctgtttttt ttctaaagcg
     1501 gtttttacgg gttgaaaact ttggcgatga a
//
LOCUS       DAOSGN010000245         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_85580_length_1528_cov_3.367278, whole genome shotgun sequence.
ACCESSION   DAOSGN010000245 DAOSGN010000000
VERSION     DAOSGN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85580_length_1528_cov_3.367278"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PK087_03740"
     CDS             complement(<1..305)
                     /locus_tag="PK087_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOH18417.1"
                     /translation="MNLRQFWFKYRHLAERLNKETSAVRLLISEPLSLSKTDFYLQQN
                     EILNEDQLALLERALFRYLYQNEPVQYIIGYTYFYQLKLKVNSDVLIPRPETEELVD"
     gene            complement(310..1341)
                     /gene="alr"
                     /locus_tag="PK087_03745"
     CDS             complement(310..1341)
                     /gene="alr"
                     /locus_tag="PK087_03745"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOH18418.1"
                     /translation="MNNLLYQTIDLKAIGANWDAIKAKSQKDIIAVVKSRCYGLGDQV
                     IPKFLESKGCKILAVVDEQEALRLLNLQIKTGVLILNSVSESFYDEANTYPNLIFSVN
                     NLIDAFKLSKKIFKRQVKLHVQIDTGMNRLGFTKMADYKQALTLLAQNPCLELEGLYT
                     HFTSPENREAQEARFRPYLKTFPYTVIHCAASSTYEYSQIGNFVRVGLDLYGDGRKAH
                     IKQSVTVGCFPLAVNFVRKGETIGYDQDYQVTEDMYVAVLPLGYYNGFRRSLQGMVVL
                     AKQRRYESIGIVCMNHLFVRVDQDVTTETEFIITSRELPNCELAQYLNTTPHEIWCML
                     NIDNKIYLE"
     gene            complement(1353..>1528)
                     /locus_tag="PK087_03750"
     CDS             complement(1353..>1528)
                     /locus_tag="PK087_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOH18419.1"
                     /translation="GDRSEKIRTYNFPQNRVTDHRIGFTIQQLDRVMEGRLDPIIEAL
                     ITEDQQRILAKQE"
BASE COUNT          482 a          304 c          279 g          463 t
ORIGIN      
        1 aaatcaacta attcttcggt ttccgggcgg ggaatgagca catcggaatt aacttttagt
       61 ttaagctgat aaaaataagt ataaccgata atatattgca caggttcgtt ttggtacaaa
      121 taacgaaaga gggctcgttc caaaagcgca agctgatctt catttaatat ttcattttgt
      181 tggagataaa aatctgtttt tgataaggat aaaggttcac taatcaacaa gcgaacggcg
      241 gaagtttcct tatttaaacg ctcagcaaga tggcggtatt taaaccaaaa ttgtcgtaaa
      301 ttcatcatct tattcaagat aaatcttgtt gtcaatgttt aacatacacc aaatttcatg
      361 cggtgttgtg tttaaatatt gcgctagttc acagtttggc agttccctag aagtaataat
      421 aaactcggtt tccgtcgtta catcttgatc aacacgcaca aacaaatgat tcatgcaaac
      481 aatcccgata ctttcataac gacgctgttt ggctaaaacg accattcctt gtaaactgcg
      541 ccgaaaaccg ttgtaatagc caagcggtaa aaccgcgaca tacatatctt cagtgacttg
      601 ataatcttga tcataaccga tggtttcacc tttcctaaca aaattaacag ccaaaggaaa
      661 gcagccaact gtcaccgatt gtttgatatg agccttacga ccatcaccat ataaatccaa
      721 acccacccga acaaaattgc cgatttgaga atattcatag gttgaagatg cagcacaatg
      781 aataacggta taaggaaagg tttttaaata aggccgaaaa cgcgcttctt gggcttcacg
      841 gttttcaggc gaagtaaaat gcgtatacaa tccttccaat tccaaacaag gattttgggc
      901 aagcaaggtt aatgcttgct tataatccgc catcttggta aaacctaagc ggttcatccc
      961 ggtatcaatt tggacatgga gtttaacttg ccgtttaaaa atttttttcg ataacttgaa
     1021 ggcatctatt aggttattga cggaaaaaat aagattcggg taagtattgg cctcatcata
     1081 aaaactttca ctaacactgt ttaaaatcaa aactccggtt ttgatttgca agttaagcaa
     1141 tcttaaggct tcttgttcat caaccacagc taatatttta caacccttgc tctctaaaaa
     1201 cttggggatc acttgatcac ccaagccata gcaacggctt ttcacaaccg caataatatc
     1261 tttttgggat ttagctttaa tggcatccca attagcaccg atagctttta agtcaatggt
     1321 ttggtaaagt aaattattca cattaacctc ttctattctt gtttcgcaag aattcgttgt
     1381 tgatcttcgg taatcaaagc ctcaataata ggatctaggc gtccttccat cacacgatca
     1441 agttgttgaa tggtaaaacc gatgcgatga tccgtaactc ggttttgggg gaaattatat
     1501 gtgcggattt tttcactgcg gtcgccgg
//
LOCUS       DAOSGN010000246         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_85670_length_1527_cov_2.898777, whole genome shotgun sequence.
ACCESSION   DAOSGN010000246 DAOSGN010000000
VERSION     DAOSGN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85670_length_1527_cov_2.898777"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PK087_03755"
     CDS             <1..545
                     /locus_tag="PK087_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18420.1"
                     /translation="IYAINSLRETGILPSLPSGDEQAMLETEPIKAYLHVEPMKQSPY
                     QRPEAPVTESPFEPVFGQEQARIAQELKQAEPIMQSYTELKIDQIERRMYEQEKRLQE
                     KDAKIKELERRALQMKLEQDVNNLVGQNLEMLKDMNYIEKNSEKIMMSKELKDLYKGL
                     ITNVDPKYRHLKEEKNDKEN"
     gene            529..1467
                     /locus_tag="PK087_03760"
     CDS             529..1467
                     /locus_tag="PK087_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH18421.1"
                     /translation="MTKKIKDWEVGDKATLFLRFSDIQIRKTSANADYATMLGFDGTD
                     LIEVKMWSFNDEKRALLKNGEIYEASGTMKDYQGKMQFNVTDFRLALPSEINPQDFYE
                     YAKLDEETLKAEIVGYVNRIDNETIKTIVYQLLKKYFADYFLFPAAMNVHHNYFSGLA
                     YHVYSMLKVSDAYLDLFPFLNRSLVYGGIILHDLGKLVELSGPKGTTYTKAGNLLGHI
                     TIAANLLYEVAKSLNVLESDEYLSLSHMVLSHHGYLEYGSPKEPIIPEAALLYMLDFA
                     DSRLGALEKEVANTEKGEYTNPILAFDRKSFYVPKL"
BASE COUNT          512 a          272 c          320 g          423 t
ORIGIN      
        1 aaatctacgc cattaattca ctgcgggaaa ccggcatttt accgtcatta ccaagtggtg
       61 atgaacaagc tatgcttgaa acagaaccga ttaaagctta tttacatgtt gaaccgatga
      121 aacaaagccc ttatcaaagg ccggaagccc ctgtaactga aagccccttt gaaccggttt
      181 tcggtcaaga acaagcccga attgctcaag aactcaaaca agccgaaccg attatgcaaa
      241 gttatacgga attgaaaatt gaccaaattg aacggcgaat gtatgaacaa gaaaaacgtc
      301 ttcaggaaaa ggatgcgaaa attaaagagt tggaacgccg agccttgcaa atgaaattag
      361 agcaagatgt caacaattta gtcggtcaaa acttggaaat gttgaaagac atgaattata
      421 ttgaaaaaaa cagcgaaaaa atcatgatgt cgaaagaact gaaagatctt tataagggtt
      481 tgataactaa tgttgacccg aagtaccgtc atctaaaaga ggaaaagaat gacaaagaaa
      541 attaaagatt gggaagttgg cgacaaagcg acgctctttt tacggttttc cgatattcaa
      601 atcagaaaaa ccagcgccaa tgccgattat gcgacgatgc tcggttttga cgggactgac
      661 ttaattgaag tcaaaatgtg gtcatttaat gatgaaaagc gagccctttt gaaaaacggt
      721 gaaatttatg aagccagcgg caccatgaaa gactatcaag ggaaaatgca gttcaacgtt
      781 actgattttc gtttagccct tccctccgaa atcaacccgc aagattttta tgaatatgct
      841 aaattggatg aagaaacctt aaaagcggaa atcgtgggct atgttaatcg gattgacaat
      901 gaaacaatta aaacgattgt ttatcaactg ttaaaaaaat actttgccga ttattttttg
      961 tttcccgccg ctatgaatgt gcatcataat tatttttccg gtttagctta tcatgtttat
     1021 tcaatgttaa aagtcagtga tgcttattta gatttatttc cgtttttaaa ccgcagtttg
     1081 gtttatggcg ggattattct tcatgatcta gggaaattag tcgaattaag cggaccgaaa
     1141 gggacgactt atacaaaagc agggaatctg ttaggccaca ttaccattgc cgccaacttg
     1201 ctttatgaag tggcgaaaag cctcaatgtt ttagaaagtg acgaatactt gagtttaagc
     1261 catatggttc tttcccacca tggttatttg gaatacggtt caccgaaaga accgataatt
     1321 ccggaagcgg ccttgcttta tatgctggat tttgctgatt cccgtttagg ggctttggaa
     1381 aaagaagtcg ctaatacgga aaaaggcgaa tatactaacc cgattttagc ctttgatcgc
     1441 aaatcctttt atgttcccaa actgtaataa atccaccttt agggtggatt tttgctttga
     1501 cgggggagaa atacatgtaa aaactgg
//
LOCUS       DAOSGN010000247         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_86122_length_1522_cov_2.514656, whole genome shotgun sequence.
ACCESSION   DAOSGN010000247 DAOSGN010000000
VERSION     DAOSGN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86122_length_1522_cov_2.514656"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PK087_03765"
     CDS             <1..689
                     /locus_tag="PK087_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18422.1"
                     /translation="GEVAKYLDEDTINRLSNDELYQEIVSTLSIDEFSSKQLFKSSHR
                     AEYLERAVFFCPVCRRIGTLHSHKDMLHCTYCHLEVTYQENLSLTANNQLFKFTYLWE
                     WMEFEKDYLLELDLSENRTLFKDEKVTLKEVIKNRRKKKLLKGQMMMDFENIKVTDGE
                     KTLMFPLDDIISMAVMGQNKLNIYFGKAIFQIKAHPRFNALKYMQLYFHIKNKKKGVT
                     DGDLARFLGI"
     gene            658..>1522
                     /locus_tag="PK087_03770"
     CDS             658..>1522
                     /locus_tag="PK087_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HOH18423.1"
                     /translation="MEIWQGFWEFSIGIDQMMLQLGIMIVAFLIGNMLRRKVGFIRKS
                     LIPSSIIGGIIILLLKIFPFFRNILTKEFMEVITYHALGLGTIALSLKASPKGPAKGR
                     NKEIMNAGLITVNSYLIQGIFGALITVLLAVTFMKGLLPASGLILPLGFGQGSGQALS
                     WGTIYETEWGFTGGASFGLTVAAIGFLVACLFGLTHIIVQKKRGKIVIGDQSDALVQL
                     EEFTSPNDVPLTESVDRFTIQMALIILVYFLTFLTIWGVVKLDAGNFVDKTVKPMIVG
                     FNFLIGTFFAIL"
BASE COUNT          444 a          257 c          327 g          494 t
ORIGIN      
        1 ctggggaagt agcaaaatac ttagacgaag atactattaa tcgtttaagc aacgatgaac
       61 tttatcaaga aattgtgtcg actttaagta tcgatgagtt ttcttctaaa caattgttta
      121 agagttctca tcgagccgag tatttggaac gggcggtttt cttctgtccg gtgtgtcgta
      181 gaatcgggac gcttcattcc cataaggata tgttacattg tacttattgc catttagaag
      241 taacttatca agaaaactta agtttaacag ctaacaatca gttgtttaag tttacttatc
      301 tatgggaatg gatggaattt gaaaaggatt atttattaga acttgattta agcgaaaacc
      361 gaaccctctt taaggacgaa aaggttactt taaaagaagt cattaaaaac cgccgcaaga
      421 aaaaactctt aaagggacaa atgatgatgg attttgaaaa tatcaaagtc acagatggcg
      481 aaaaaaccct catgtttccg cttgatgata ttatctcgat ggctgttatg ggacagaata
      541 aactgaatat ttattttggg aaagccatct ttcaaattaa agctcatcca cgctttaatg
      601 ctttaaaata tatgcaactg tattttcata ttaagaacaa gaaaaaagga gttaccgatg
      661 gagatttggc aaggtttttg ggaatttagt attggcatcg atcaaatgat gctccaatta
      721 ggaattatga ttgtggcttt tttaattggc aatatgctta gaagaaaggt cggttttatt
      781 agaaagtcat taatcccgtc ttcgattatt ggtgggatca ttatcttatt attaaaaatt
      841 tttccgtttt tccgtaatat actgacaaag gagtttatgg aagtcatcac ttaccatgcc
      901 cttggtttag gaacgattgc cttatcgctt aaggccagtc cgaaaggacc ggctaaaggc
      961 cgcaacaaag aaatcatgaa tgccggttta attaccgtca attcctatct tattcaagga
     1021 atcttcggtg cgctcattac cgttctttta gcggtgacct ttatgaaggg cttgcttccg
     1081 gcgtcaggat taatattacc actgggtttt ggtcaaggtt ccggtcaagc cttatcgtgg
     1141 ggcacgattt atgaaaccga atggggtttt accggcgggg ccagctttgg tttaaccgtc
     1201 gctgccattg gttttttagt ggcttgttta tttgggctaa ctcatatcat tgtgcagaag
     1261 aagcggggca aaatcgtcat tggtgaccaa agcgatgctt tagtgcaatt ggaagaattc
     1321 acttcgccca acgatgtacc gctaacggaa tcggtggacc gctttaccat tcaaatggct
     1381 ttaattatct tggtttactt tttgaccttt ttaacgattt ggggagtagt gaaattagat
     1441 gccggaaact tcgttgataa gactgtcaaa ccgatgattg taggctttaa ctttttaatc
     1501 gggaccttct ttgcgattct tg
//
LOCUS       DAOSGN010000248         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_86250_length_1520_cov_3.725597, whole genome shotgun sequence.
ACCESSION   DAOSGN010000248 DAOSGN010000000
VERSION     DAOSGN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86250_length_1520_cov_3.725597"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1101
                     /gene="holA"
                     /locus_tag="PK087_03775"
     CDS             145..1101
                     /gene="holA"
                     /locus_tag="PK087_03775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOH18424.1"
                     /translation="MANIYLYTGEEKYRINTKIQGLVKEAGADEYNIMSYDLDDVEIG
                     DAIRDALTPPFMCSQKVVIVKNPRFLTAEKSLTEEQANDFLKYLTKPMDTTVFIINAV
                     NLKLDERKEVVKRLKKVAQISDNNELTEVEFYGWVKRQCVINDVDIKDDATRVFYQLA
                     GKTLMNAKNELDKLINYVGDKGLITVDVVNKVVVKEIQNDVFALANAIIDHNRPKVIN
                     LYRDLTKIGNDVNFLFALVAKSMRELLAVSLMLKNGYKQQEVANTLNVSSGKAFYLVK
                     NARSMNLSTIEKYVQKLGELDYKIKSGLIDAKTGFEFFLFEI"
     gene            complement(1160..1423)
                     /gene="rpsT"
                     /locus_tag="PK087_03780"
     CDS             complement(1160..1423)
                     /gene="rpsT"
                     /locus_tag="PK087_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOH18425.1"
                     /translation="MANIKSQAKRNLTNEKKRLQNASFKSSLRTAIKNVELAVQKKDL
                     EEAKKAYQLASAKLDKSIVKGLFHKNYVARNKSRLSKLVNTLA"
BASE COUNT          504 a          229 c          298 g          489 t
ORIGIN      
        1 atcaacatgt cggacaaaac ccgaaataaa gagtttgaaa attatcaaaa ggattattat
       61 tttgtcttag tgatttatga ttatgaagct cgaacattag ttatttatga actttattct
      121 taaaagaaaa gtgatggtgc taagatggct aatatttatt tatataccgg tgaagaaaaa
      181 tatcggatta atacgaaaat tcaagggctt gtgaaagagg cgggggccga cgaatacaat
      241 atcatgagtt atgatttgga tgatgtggaa atcggcgacg ccattcgtga tgccttgaca
      301 ccaccgttta tgtgttcaca aaaagtcgtg attgtgaaga atccacgctt tttaactgcg
      361 gaaaaaagtt tgacggaaga acaagccaat gattttctca agtatttaac taaaccgatg
      421 gacaccaccg tgttcatcat caatgccgtt aatttaaaac tcgatgaacg caaagaagtt
      481 gtgaagagat taaaaaaagt cgcccaaatt agcgacaaca acgaactgac agaagttgag
      541 ttttatggtt gggttaaacg gcaatgtgtc attaacgatg ttgatattaa agatgatgcc
      601 acccgggttt tctatcaact agctggaaaa accttgatga atgcgaaaaa cgaattggat
      661 aaattgatta attatgtggg tgataaaggc ctgattacgg ttgatgttgt caacaaagta
      721 gtggtgaaag aaatccaaaa tgatgttttt gctttagcca atgccattat tgatcataac
      781 cgccccaaag taatcaattt gtatcgagac ttaaccaaaa tcggtaatga tgtcaatttt
      841 ttgtttgctt tggttgcgaa aagcatgcgt gaattattag ccgtcagttt aatgcttaaa
      901 aatggctata aacagcaaga agttgctaac actttgaatg tgtcttccgg gaaagccttt
      961 tatttagtta agaacgcccg cagcatgaat ttatctacca ttgaaaaata tgttcaaaaa
     1021 ttaggcgaat tggattataa aatcaaaagc ggattaatag atgcgaaaac cggttttgaa
     1081 ttttttcttt ttgaaatata ggcgctatta agcgcttttt gtataaaaaa acacccatcg
     1141 aacaagtgtc ttgggtgatt tacgctaaag tatttactaa tttggaaagt cgggacttat
     1201 tgcgcgccac atagttttta tggaataatc ccttaacaat ggatttatct aatttggcgc
     1261 tcgctaattg gtaagctttt tttgcttctt caagatcctt tttttggaca gccaattcca
     1321 catttttgat ggccgttcgc aaagaagatt tgaaagaggc attttgcagt cttttctttt
     1381 catttgttag attacgttta gcttgtgatt taatgttagc catgatttat acctccaaat
     1441 gatacatgtc atattatact aaaaatcgtt tacaaaagca agtaaaatta cgattaaaat
     1501 ttttgtaaac gatttttagt
//
LOCUS       DAOSGN010000249         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_86382_length_1519_cov_2.572404, whole genome shotgun sequence.
ACCESSION   DAOSGN010000249 DAOSGN010000000
VERSION     DAOSGN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86382_length_1519_cov_2.572404"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="PK087_03785"
     CDS             <1..178
                     /locus_tag="PK087_03785"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945845.1"
                     /note="catalyzes the formation of acetyl phosphate from
                     acetyl-CoA and phosphate; can also act with other
                     short-chain acyl-CoAs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOH18426.1"
                     /translation="GNVFYKTSVFLGRAESAGLVLGAKCAIVLTSRADSAQSKLYSIA
                     LAAVYQNGLSHLSY"
     regulatory      349..450
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            555..>1519
                     /locus_tag="PK087_03790"
     CDS             555..>1519
                     /locus_tag="PK087_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOH18427.1"
                     /translation="MENFFKLKENGTTWSKELVAGATTFFAMVYIIFVNPAMLEQTGM
                     PIGGVFLATIFATVIGTLVMGLFANVPYALAPGMGLNAFFTYTVCFGFGFSWQEALLL
                     VFVCGLINIVITVTKVRKAIIRAIPKSLQHAIGGGIGLFIAYIGFKNAGLLKFTADPG
                     HWTESFGTAFIDSGVVPALTDFNTLVPVLGIIGIVIMVVLLLLKVRGAILIGIIVTAI
                     IGIPMGITNLKGINFDIAAPFRDLGDTFLAAFGPKGLGTLIGDVKRWPMALLAIFAFS
                     LTDTFDTIGTFIGTGRRSGIFTDEDLLAVEDGKGFATKMEKALFA"
BASE COUNT          388 a          264 c          322 g          545 t
ORIGIN      
        1 ggggaatgtc ttttataaaa cttccgtctt tttaggaagg gcggaatccg ccggtctcgt
       61 tttaggagcg aagtgtgcga ttgtcttaac cagtcgggcc gactccgccc aatcaaaatt
      121 atattccatt gctttagcag cggtttatca aaacggctta tcccatctta gttattagct
      181 gtacttggtc cctaaaaatg ctttgttttt agatgatcaa ttgctttgat tcttttaaag
      241 ctaatgctaa tattaagctt gtttattgtt tttttaccaa tgaaaacctt tacttagggt
      301 tgcattattg ctgattttgt agtattatct agacatggtt ttgtgaccgt gaatttaata
      361 gcattcgcca atataatatg gataatatgg ttccagagtt tctaccaagc gaccttaaat
      421 tgcttgacta ttggttaatt caccacagtg ttttggcatg tggttttatt ggcgagtata
      481 gaaatatact tgctttttct tttacgctca aaactaaata tgagagaaag gtcatcaaag
      541 attagatgaa caaattggaa aactttttca agttgaaaga gaatggaaca acttggtcaa
      601 aagaattagt tgccggtgca accaccttct ttgccatggt ttacattatc tttgttaacc
      661 ctgccatgct tgaacaaacc ggaatgccta ttggtggtgt gttcttagca acgattttcg
      721 caacggttat tggaacttta gtaatgggtt tattcgccaa tgtcccctat gccttagccc
      781 caggaatggg attaaatgcc tttttcactt atacagtttg ttttggtttt ggattttctt
      841 ggcaagaagc attgctattg gttttcgttt gcggattaat caacatcgtc attacggtca
      901 ctaaagtaag aaaagctatc atccgcgcga ttcccaagag tttgcaacat gccattggcg
      961 gcgggattgg tctttttatt gcctacatcg gttttaaaaa tgccgggctt ttaaagttca
     1021 ctgctgatcc gggtcattgg acggaatcat tcggcaccgc ttttattgat tctggtgttg
     1081 tccctgcttt aaccgatttt aacactctag ttcctgtttt aggaattatt ggaattgtta
     1141 ttatggtagt tttattgtta ttaaaagtaa gaggtgctat tttaattggt attattgtaa
     1201 ccgcaatcat cggtattccc atgggaataa ccaatttaaa gggcattaat tttgatattg
     1261 cggcgccttt tagagattta ggcgatactt tcctagctgc gtttggcccg aaaggtttgg
     1321 gaacattaat cggcgatgtt aagcgttggc ccatggcttt attagctatt tttgctttta
     1381 gtttaacgga tacctttgat accattggca cctttatcgg tactggtcgt cgttcgggga
     1441 tctttaccga tgaagattta ttagctgttg aagatgggaa aggctttgct actaaaatgg
     1501 aaaaagcctt atttgcgga
//
LOCUS       DAOSGN010000250         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_86384_length_1519_cov_2.509563, whole genome shotgun sequence.
ACCESSION   DAOSGN010000250 DAOSGN010000000
VERSION     DAOSGN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86384_length_1519_cov_2.509563"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1519)
                     /locus_tag="PK087_03795"
     CDS             complement(<1..>1519)
                     /locus_tag="PK087_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18428.1"
                     /translation="LVVSLGPDDVRGEAEIKAFVRAVKVVLGESIDLNNINVDPDVLL
                     DLSNERGGADDDVGVIMSSTIITDTIIKKIIDLAEGENSSIVINLTQEDDRWYDSETE
                     DGEIRKLIRAIQIIFSGEGKSLNNPDIDPNVILNLSDGSVDPSDDQIGDILASIIASD
                     SMIKVIYDLGQGDDSALVINLTLTDERWYDTELSDGELKRFIRAVKIILGENPDLSNV
                     NVDPDILLDLSNHRGGADDDVGVIMSSTIVTDTIIKEIIDLGSAENSSIVVNLTREDE
                     RWYDKETEDGEIRRLIRAIQILFSGEGKSLNNPDIDPNVILNLSDGSVDPSDDQIGDI
                     LASIIASDSMIKVIYDLGQGDDPVLIINLTVNDARWYDDGDTRGELRHFIEAVKVILG
                     ENPNLNNPNVDANVLKTLSLGDVDPADDDVKTIQKSIIARDTIINKIIEIAHPTEGEA
                     TLVVNLEADDPRWLDSETEDGEIRKLIRAIQIIFKDPEDDINNPNIGPNIVLELTN"
BASE COUNT          477 a          351 c          232 g          459 t
ORIGIN      
        1 cattagttaa ttctaaaaca atattggggc caatattggg attattaata tcatcttccg
       61 gatctttaaa gatgatttga atggcgcgaa ttaattttct aatttcgcca tcttcggttt
      121 cactatcgag ccatcttgga tcatcggctt ccaaattaac aactaaagtc gcttcacctt
      181 cagtcggatg agcaatctcg ataatcttat tgataatagt atcccgagca ataatggatt
      241 tttgaatcgt tttaacatca tcatcagccg ggtcaacatc acctaaactt agggttttta
      301 aaacattggc atcaacattc ggattattta aattcgggtt ttcacctaaa ataactttaa
      361 cagcctcaat aaagtgccgc agttcacccc tagtgtcacc atcatcatac catctggcat
      421 cattaacagt taaattgata atcaagaccg gatcatcgcc ctgacccaaa tcataaataa
      481 ctttaatcat tgagtcggaa gcaataatcg aagctaaaat atcaccgatt tggtcatcac
      541 ttggatcaac tgagccatcg gataaattca aaatcacatt cggatcaata tccggattat
      601 ttaagctctt tccttcaccg ctgaagagaa tttgaatggc gcggattaat cttctgattt
      661 caccatcttc cgtttctttg tcataccaac gttcatcttc ccttgttaag ttcacaacaa
      721 tcgaagaatt ttcagcactg cctaaatcaa tgatttcctt aataattgta tcagttacaa
      781 tggtggatga cataatcaca cccacatcat catcagcgcc accacgatga ttagataaat
      841 ccagcaagat atccggatca acattgacat tggataaatc cgggttttcc cctaaaatga
      901 ttttaacagc ccgaataaag cgtttcaatt cgccatcgct caattccgta tcataccaac
      961 gctcatcagt gagtgttaag ttaataacaa gggccgaatc atcgccctga cccaaatcat
     1021 aaataacttt aatcattgaa tcggaagcaa taatggaagc taaaatatca ccgatttggt
     1081 catcacttgg atcaactgag ccatcggata aattcaaaat cacattcgga tcaatatccg
     1141 gattatttaa gctctttcct tcaccgctaa agataatttg gatagcacgg attaattttc
     1201 tgatttcccc gtcttccgtt tcgctgtcgt accaacgatc atcttcttgc gttaaattaa
     1261 tgactattga tgagttctca ccctcagcta aatcaataat ctttttaata attgtatcag
     1321 taatgatggt ggatgacata atcaccccga catcatcatc ggccccacca cgctcattgg
     1381 ataaatccaa taaaacatct gggtcaacat tgatattatt taaatcaatg ctttcgccga
     1441 gcacaacttt cactgcccgc acaaaagctt tgatttccgc ttccccgcga acatcatcgg
     1501 gacctaaaga aacgactaa
//
LOCUS       DAOSGN010000251         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_86806_length_1514_cov_2.828650, whole genome shotgun sequence.
ACCESSION   DAOSGN010000251 DAOSGN010000000
VERSION     DAOSGN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86806_length_1514_cov_2.828650"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..966
                     /locus_tag="PK087_03800"
     CDS             139..966
                     /locus_tag="PK087_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH18429.1"
                     /translation="MKLLTFGELILLAAIVIVPIVYLVGSSFAKTSGLPTTFWPSKMT
                     WDNYKKLFTETKFWYWYKNTITIAVLNMVFGVLFITGAAYVFARFKFKGKKVGLITIL
                     VLQAFPSFMGLIAMFTLFDTFGLLNKPWALTILYVGGAIPFNLWLIKGYLQQIPKDLD
                     ESAMLDGANKWQIFSKIILPLSVPILSFVAVSQFMAPWMDYMLPNYLLNSPDYSGDIQ
                     EVWTLAIGLFSMISGQDKANYTTFAAGALIVALPITLLYMAFQRFLIQGITAGATKG"
     gene            1016..>1514
                     /locus_tag="PK087_03805"
     CDS             1016..>1514
                     /locus_tag="PK087_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH18430.1"
                     /translation="MDKNWWKEAFGYQIYLRSFFDGNNDGIGDLPGIIAKLPYLAHLG
                     VNLLWICPFYTSPMDDNGYDVANFYRVAREYGTLKDLRSLIKSAHQLQMKVIIDLVLN
                     HTSDEHPWFKASRKSLNNPYRDYYIWKKPRIIDGQQTEPTNWASFFGGSCWEFDVRTQ
                     EYYMKI"
BASE COUNT          390 a          299 c          314 g          511 t
ORIGIN      
        1 aaaggttatt caagatatcg cggtgccctc tggtctttaa ttcctattta tggtataata
       61 cattgttttc gggctccggc cattaaagct gttcaaaaag gcggattgaa acaatttttt
      121 tccgtcaaag aaatcttcat gaagttactt acttttgggg aattaatttt gctggcggcg
      181 attgtcattg tgccgattgt ttatttggtc ggctcatcct ttgcgaagac gagtggttta
      241 ccgacgacct tttggccctc gaagatgact tgggacaatt acaaaaaatt atttacggaa
      301 actaaattct ggtattggta taaaaacacg attaccattg ccgttttaaa catggttttt
      361 ggggtcttgt ttattaccgg tgcggcctat gtttttgccc gctttaaatt taagggcaag
      421 aaagttggct taattactat tttggttctc caagcttttc cgtccttcat gggtcttatt
      481 gctatgttca ctttgttcga tacctttggt cttttaaata agccctgggc cctaaccatc
      541 ctctatgtcg gtggtgctat tccctttaat ttatggctta ttaaaggtta tttgcaacag
      601 attcccaaag acctggacga aagtgcgatg ctcgatgggg ccaacaagtg gcaaattttt
      661 tccaaaatta ttttgccctt atcggtgccg attctctcgt ttgtggcggt ttcccaattc
      721 atggctccat ggatggacta tatgttaccg aattatttat taaacagccc ggattattcc
      781 ggtgatattc aagaagtttg gactttagca atcggattat tctcgatgat ttccggtcag
      841 gataaagcca attatacgac ctttgcggcg ggggccttaa ttgttgcctt gccaatcaca
      901 ttattatata tggctttcca acgcttcctc attcaaggaa ttacggcggg agctacaaaa
      961 ggttaattag gaaagacatg gtggtgtcac catgtcttcc tttttaggag cacttatgga
     1021 caaaaattgg tggaaagaag cctttggcta tcaaatatat ttacgcagtt tttttgatgg
     1081 caataatgac ggcatcggtg atttaccggg gattattgcg aaacttcctt atctagctca
     1141 cttaggcgtt aatcttcttt ggatttgtcc tttttatact tccccgatgg acgacaatgg
     1201 ttatgatgtc gctaattttt accgagttgc aagggaatac ggcaccttaa aggatcttcg
     1261 ctcccttatc aaaagtgctc accaattaca gatgaaagtc attattgact tggttttaaa
     1321 ccacacttcg gatgaacatc cctggtttaa agccagtcgt aagtccctta acaaccctta
     1381 tcgtgattat tatatttgga aaaaaccccg aataattgac ggccaacaaa ctgaaccaac
     1441 caattgggcc tcttttttcg ggggcagctg ttgggaattt gatgttcgca cccaagaata
     1501 ttatatgaaa attt
//
LOCUS       DAOSGN010000252         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_87102_length_1511_cov_2.077610, whole genome shotgun sequence.
ACCESSION   DAOSGN010000252 DAOSGN010000000
VERSION     DAOSGN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87102_length_1511_cov_2.077610"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PK087_03810"
     CDS             complement(<1..487)
                     /locus_tag="PK087_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOH18431.1"
                     /translation="MFNNVTDLITWIEAQKRHSPKVSMEKMRKLSAIYGNPHEGIKYI
                     HVGGTNGKGSTVSFIKGILRAAGFNVGSYISPYVISFNERISYNDEFIADSDLLEIGN
                     YLLAKYPMIEQQEIELPTFFEFVTLIAYVYFSRIKNLDFVTLEVGLGGRLDATNIVTP
                     LV"
     gene            complement(508..>1511)
                     /locus_tag="PK087_03815"
     CDS             complement(508..>1511)
                     /locus_tag="PK087_03815"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH18432.1"
                     /translation="DSLRYFLTTSSTPGQDIRYSEEKIEAAWNFINKLWNISRYVGIS
                     FASAKFKNEPINIEFLNEIDKWIMSKLNEVILAADENLEKYEFGEAAKAIYKFTWNDF
                     ASWYIEMSKVVFQEGTPEQKINTCAVLKHVLVAILKLLHPFMPFVTEEIYQAFNEGSI
                     VISKWPEIYQALPDVNARYVDMLFEVISTIRLVRAEKNVPNSKPIDIEIQFTDKKAFS
                     FFDDNSHYLKKFSNYAHLTLSTAELETSKKIVHVLNGLILMIPASQLVDVEQEKEKLL
                     LNQERLKKEILRCEGMLNNPHFLNKAPIDKINSEKSKLFDYQKQLEETNKLLQEFDE"
BASE COUNT          494 a          274 c          238 g          505 t
ORIGIN      
        1 ataccaaggg ggtgacaata ttggttgcat ctagtcttcc ccctaatccc acttcaaggg
       61 tgacaaaatc caaatttttg atgcgcgaaa aataaacata ggcgattaaa gttacaaatt
      121 caaaaaaagt cggtaattca atttcttgtt gttcaatcat tggatatttc gcaagcaaat
      181 aattgccgat ttctaacaaa tcggaatcgg cgataaattc atcattataa gaaatccgtt
      241 cattgaaact aatcacatag ggagatatat aggagccgac attaaaaccg gccgccctta
      301 atattccctt aatgaaagaa accgttgaac ctttgccatt ggtaccaccc acatggatat
      361 atttaattcc ttcatgagga ttaccataaa tggcactcaa ttttcgcatt ttttccatcg
      421 acactttagg tgaatggcgt ttttgcgctt caatccaagt aattaagtcc gtaacattat
      481 taaacatatc atcctttttc tttgatgtta ttcatcaaac tcttgaagta atttgtttgt
      541 ttcttcaagt tgtttttgat aatcaaataa tttgctcttt tcactgttga ttttatctat
      601 tggggcttta ttgagaaaat gaggattgtt gagcatgcct tcacagcgaa ggatttcctt
      661 ctttaagcgc tcttgattaa gtaataactt ttctttttct tgttcgacat caaccaactg
      721 ggatgcagga atcattaaaa ttaaaccatt taaaacatga acgatttttt tagacgtttc
      781 cagttccgct gttgaaaggg ttaaatgagc ataattggaa aattttttaa gataatggga
      841 attgtcgtca aaaaaggaaa aagctttttt atccgtaaat tggatttcaa tatcaatcgg
      901 tttcgaattg ggaacatttt tttccgctct tactaatctg attgtggaaa taacttcgaa
      961 caacatatca acataacgag cattaacatc gggtaaagct tgataaattt ccggccattt
     1021 actgataaca atactgcctt cattgaaagc ttggtagatt tcttcggtaa caaagggcat
     1081 gaaaggatgg agtaatttta atatcgccac tagaacatgc tttaaaaccg cacaagtatt
     1141 gattttttgt tcaggagtac cttcttgaaa aaccacttta gacatctcaa tataccaaga
     1201 cgcaaagtcg ttccaagtaa atttataaat cgccttggcc gcctctccaa attcatattt
     1261 ttccaagttt tcatcagcag ctaaaataac ttcatttaat ttgctcataa tccatttgtc
     1321 aatttcattt agaaactcaa tgttaattgg ttcattctta aatttagcac tagcaaaact
     1381 aataccaaca taacgagaaa tattccacaa tttattaata aaattccaag ctgcttcgat
     1441 tttttcctct gaataacgaa tatcttgacc gggagtagat gaagtcgtaa gaaaataacg
     1501 aagactgtca a
//
LOCUS       DAOSGN010000253         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_87328_length_1508_cov_3.029594, whole genome shotgun sequence.
ACCESSION   DAOSGN010000253 DAOSGN010000000
VERSION     DAOSGN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87328_length_1508_cov_3.029594"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK087_03820"
     CDS             complement(<1..211)
                     /locus_tag="PK087_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18433.1"
                     /translation="MRNFWILLKYNLINTLNLNKLNSKTKEGKLSLLMVILFIVGGLF
                     SLGLAFLYMFMIGSALAEGGFPVAQG"
     gene            complement(204..917)
                     /locus_tag="PK087_03825"
     CDS             complement(204..917)
                     /locus_tag="PK087_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18434.1"
                     /translation="MLEIKNLSKTYPGGKLAVDDVSLTINDGEIFGFIGHNGAGKTTT
                     LKCAVGILDFEKGDILLDGKSIKKQAIECKQEIAYIPDNPDLYEQLTGIQYLNFICDI
                     YRVSTADRNVLISQYADMFEIKKNLGDLISSYSHGMKQKLALISALVRKPKLFVLDEP
                     FVGLDPKAAFQVKELMKELCANGSCVFFSTHVLEVAEKLCHRVAIIQKGKIVAYGTME
                     EVKGDKSLEDVFLELVNNA"
     gene            complement(1004..1381)
                     /locus_tag="PK087_03830"
     CDS             complement(1004..1381)
                     /locus_tag="PK087_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOH18435.1"
                     /translation="MKTIMTNQAPKAVGVYSQGVVAHNMLFVSGQIPFVPATMTLVSQ
                     DIKEQTKQCLENVLAIVEAAGMSKTDIVRCTVFMTNLNDFPLMNEVYEAFFQDHKPAR
                     AAVGVVALPKNVQIEIDAIAVKS"
BASE COUNT          478 a          325 c          245 g          460 t
ORIGIN      
        1 gcccctgggc tacgggaaac ccgccttcgg ccaacgccga accaatcata aacatataga
       61 ggaaagctaa accaagacta aacaaacccc caacaatgaa taagattacc attaataaag
      121 ataatttacc ttctttcgtt ttggagttta gtttatttaa atttaaagta ttaattaaat
      181 tatactttaa taaaatccaa aaattacgca ttgttaacca actccaagaa aacatcttct
      241 agggatttat cacccttaac ttcttccatc gtgccgtaag cgacaatttt tcctttttga
      301 ataatcgcca cccgatggca taatttttcc gcaacttcca aaacatgggt tgagaaaaag
      361 acacaggagc cattagcaca taattctttc atcagttctt tcacttggaa agcggctttc
      421 ggatctaaac cgacaaaggg ttcatccaaa acaaatagtt tcggtttacg gactaaggcg
      481 gaaattaatg ccaatttttg cttcatcccg tgtgaatacg aagaaataag atccccgagg
      541 ttcttcttga tttcaaacat atccgcatac tgagaaatca acacattgcg atccgccgta
      601 gaaacccgat aaatatcaca aatgaaattc aaatattgaa tccccgttaa ctgttcatat
      661 aaatcgggat tatccggaat ataagcaatt tcttgcttac attcaatcgc ttgcttttta
      721 atggatttgc catccaaaag aatatcgcct ttttcaaaat ccagaattcc gaccgcacat
      781 tttaaagtgg ttgttttgcc ggcaccatta tggccgatga agccgaaaat ttcgccatca
      841 ttgatggtta aagaaacatc atcgacagct aatttcccgc cgggataagt tttagataaa
      901 ttctttattt ctaacatagg atacctcttt tctttcgtaa tattatagca ttattcttgc
      961 cttttgtcat tatcttctat gattaattat aatggaagcg attttatgat ttaacagcaa
     1021 tggcatcaat ttcaatttgc acattcttgg gtagagccac caccccgact gcagcccgcg
     1081 ccggtttatg gtcttggaaa aaagcttcat aaacttcatt catgagggga aaatcattaa
     1141 gattagtcat aaaaaccgta caacgaacaa tgtcagtttt actcatgccc gccgcttcta
     1201 caatcgctaa gacattttct aaacattgct tggtttgctc tttgatatct tgactgacta
     1261 aagtcatggt tgccggaaca aacggaattt gaccggagac aaacaacatg ttgtgagcca
     1321 caacaccttg gctataaacc ccaaccgctt tcggagcttg attggtcata attgttttca
     1381 tagaatcctc cttcttttaa aactcaatta attctaccat aagcggtgct ttttttcatc
     1441 attttccctt aaaaaggaaa atgcaattca ctagggggag tgaattgcat ttaggcccta
     1501 aaaggagc
//
LOCUS       DAOSGN010000254         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_88256_length_1497_cov_4.321775, whole genome shotgun sequence.
ACCESSION   DAOSGN010000254 DAOSGN010000000
VERSION     DAOSGN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88256_length_1497_cov_4.321775"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PK087_03835"
     CDS             complement(<1..742)
                     /locus_tag="PK087_03835"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HOH18436.1"
                     /translation="MQKIMMLVNGFWLVSIYVVVSLLLVVIGLAVGYLVFKRQYEKAG
                     KSAAKLIEDAKKVAEENRKASLIEFKQETFKLKQESEKEIRNLKQETEREIKEKRRAT
                     AELEQKLSQREERLDNRMSNLDKREENLNQKEQKIEQRKNDLDKQYSKAEELIEAQNK
                     KLLEISGLSVEDAKKIIIERVEEDMAMEVAQIIKDAEEKAKLEVNRKAQCLLANAIQQ
                     YAGEIVSETTVSVVPLPNDEMKGRIIGRE"
     gene            complement(922..1497)
                     /locus_tag="PK087_03840"
                     /pseudo
     CDS             complement(922..1497)
                     /locus_tag="PK087_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275914.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
BASE COUNT          383 a          334 c          250 g          530 t
ORIGIN      
        1 cttcacgacc aataatccga cctttcattt catcgtttgg tagtggcact accgaaacgg
       61 tggtttcgct gacgatttca ccggcatatt gttggatcgc attcgccagt agacattggg
      121 ctttgcgatt cacttcaagc ttggcttttt cttcagcatc tttgataatt tgggcaactt
      181 ccattgccat gtcttcttca acacgttcaa taataatttt tttggcatct tccacactta
      241 agccagaaat ctctaataat ttcttatttt gagcttcaat gagttcttcc gctttgctgt
      301 attgtttgtc gagatcgttc ttcctttgtt caattttttg ctctttttga tttaaattct
      361 cttcacgctt gtcaagattg gacatccgat tgtccaaacg ctcttcgcgt tgggataact
      421 tctgttctaa ctccgctgtg gctcggcgtt tttctttaat ttcacgttca gtttcttgtt
      481 ttaagttgcg gatttctttt tcgctctctt gctttaattt aaaagtttct tgtttaaact
      541 caattaaaga agctttgcga ttttcttcag caactttttt cgcatcttcg attaacttgg
      601 cagcggattt ccccgctttt tcatattgtc ttttgaaaac caagtaaccg acggcaagac
      661 cgataacgac tagcaaaagg gagacaacaa cataaatgga gaccagccaa aaaccattga
      721 ctaacatcat gattttttgc attcttttat gttcctccat aggtaattat ataattataa
      781 caattatagt ctactggttt attatacctt aaaaagcctt ttaggtcaat gaaaatatat
      841 ggtcaatttc cggcaaaatt aaaacaggca gctttctttc tgcctgtttt tggtttattc
      901 ttgcgaatga agctttcctt ttaacttctc gcggattttc atttcaattt ctttagttaa
      961 gttaggatta ttctttaaat actccttcac attttcccga ccctgaccca gtttttcgtc
     1021 attataggaa aaccacgctc cacttttatt aatgatgtcg gaggaaactc ccaaatcaag
     1081 aacttcacct tcataagaga ttcctttgcc atagattaaa tcgacttcca caaccttaaa
     1141 tggtggggcg actttgttct tgaccacttt aatgcgcgtt acattaccga taatttccgt
     1201 tcccaattta atggcatcaa tacgacggat atccaagcgc aaggaagcat agaattttaa
     1261 agcgcgcccg cccgaagtcg tttccggcga accgaacatg acaccaactt tttcacgaat
     1321 ttggttaata aagatggcaa tcgttttgct tttggaaatc gctccgctta atttgcgcat
     1381 agcttgcgac atcaatcggg cttgcatgcc gatgtgagca tcacccatat caccgtcaat
     1441 ttccactttc tgaactaaag ctgctaccga gtcaataacg ataacatcga tggcatt
//
LOCUS       DAOSGN010000255         1496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_88405_length_1496_cov_2.394171, whole genome shotgun sequence.
ACCESSION   DAOSGN010000255 DAOSGN010000000
VERSION     DAOSGN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88405_length_1496_cov_2.394171"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PK087_03845"
     CDS             complement(<1..547)
                     /locus_tag="PK087_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOH18437.1"
                     /translation="MLIKEIVQYLEQLYPPQLAIESDFNRIGFIIGDLNFEVSNVLMA
                     LDLTLEVAQEAVLKNANLIITHHPFFFQPLDKIMFSSEKGQILNLLFTHRLSLFVMHI
                     NLDVGNNGVNDTLARLIGLIDFKVINNEVGKGNFLRYGEIRPLTLASLALKLKNDLDL
                     TGVRVVGDPQKIIKRVGIVGGS"
     gene            complement(541..1182)
                     /locus_tag="PK087_03850"
     CDS             complement(541..1182)
                     /locus_tag="PK087_03850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH18438.1"
                     /translation="MISNRLQAIAAFIPPAKRIVDVGCDHGYIIIEAFQKYQIESAVA
                     LDNKAGPLSKAFNNIRGYGFFDRVNFVLSEGLEQFYAQADAFILSGMGGLNIINIINN
                     GLDKLGQARLIIQANRHAFEVRTYLTNQGYYLVNEELIFEGGKYYEIELFEKTEEPVF
                     YHEDELYFGPLLLARKSEIFMNKLRHDLRILKAISHPMPKHLKTIKRIEEILC"
     gene            complement(1251..>1496)
                     /locus_tag="PK087_03855"
     CDS             complement(1251..>1496)
                     /locus_tag="PK087_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH18439.1"
                     /translation="PLDYTINEKYKEEIDVVLRTLTPREEKVLRLRFGLTDGKPHTLE
                     EVGKEFGVTRERIRQIEAKAIRRLKHPTRKNRLNQFK"
BASE COUNT          493 a          295 c          239 g          469 t
ORIGIN      
        1 cactcccacc aacaatcccc accctcttaa taatcttttg cggatcccca actaccctga
       61 ctccagttaa gtccaaatca tttttaagtt ttagcgctaa actggctaaa gttagtgggc
      121 gaatttcacc ataacgcaaa aagtttcctt tgccaacctc attatttatg actttaaaat
      181 ctataagccc aattaaacgg gctagagtat cattaacacc attgtttccg acatctaaat
      241 tgatatgcat aacaaacaaa gaaaggcgat gtgtaaataa caaatttaaa atttgtcctt
      301 tttcacttga aaacatgatt ttatctaagg gctgaaagaa aaatgggtga tgggtgatga
      361 ttaaattagc attttttaaa accgcctctt gagctacttc taatgttagg tcaagtgcca
      421 ttaaaacatt gcttacttcg aagtttaaat ccccaatgat aaaaccaatg cggttaaaat
      481 cgctttcaat cgccaattgg ggcgggtata gttgttctaa atattggaca atttctttaa
      541 ttaacataat atttcctcaa ttctttttat tgtttttaga tgcttaggca ttggatggga
      601 aatagctttt aaaattcgta aatcatggcg aagtttattc ataaaaattt ccgattttcg
      661 tgctaataag agcggaccaa aataaagttc atcttcatga taaaaaaccg gttcttccgt
      721 cttttcgaac aattcaatct cataatattt cccaccttca aaaattaatt cttcattgac
      781 aagataatat ccttgattag ttaaataagt tcgaacttca aacgcatgcc ggttggcttg
      841 aatgattaaa cgggcttgac ctaatttatc caatccatta ttaatgatat taatgatgtt
      901 taacccgccc atgccggata aaataaaggc atcggcttgg gcataaaatt gctctaaacc
      961 ttcgcttaaa acaaaattaa ctcgatcaaa aaaaccgtat ccgcgaatat tgttaaaagc
     1021 ttttgaaaga ggaccggctt tattatctaa agcaaccgct gattcaatct ggtatttttg
     1081 gaaagcttcg ataatgatat atccatgatc acaaccaaca tcaacaatcc tttttgcagg
     1141 aggaataaaa gccgcaatgg cctgtaaacg attggaaatc ataaacacct cacgacttaa
     1201 ttatacaaaa aaagccctat gaaagcaatg aaaaacgtcc acatggacgt ttatttaaac
     1261 tggtttaaac gatttttacg agtcggatgc tttaggcgac gaatagcttt ggcttcaatc
     1321 tggcgaatgc gttcacgcgt aactccgaac tcttttccca cctcttctaa agtgtggggt
     1381 tttccatctg ttaacccaaa gcgtaaacgg aggacttttt cttcacgagg agtcaatgtt
     1441 cgtaaaacaa catcaatttc ctctttatac ttttcattaa tggtgtaatc aagagg
//
LOCUS       DAOSGN010000256         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_88961_length_1489_cov_3.755230, whole genome shotgun sequence.
ACCESSION   DAOSGN010000256 DAOSGN010000000
VERSION     DAOSGN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88961_length_1489_cov_3.755230"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..513
                     /locus_tag="PK087_03860"
     CDS             67..513
                     /locus_tag="PK087_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18440.1"
                     /translation="MKKFSLFILLIIANFVFLSASNIDASGLEKIKIEEKINYQSIED
                     DIIIFANIEDLSLLKDAKELISPDHIVIIYNLEGDVVDLLYNQEYKNTFAYIYYYNSL
                     GTLVRMRVSGTNLSKNSRKIELKKHLNLYYQKYELEKREKEMFIAS"
     gene            complement(691..>1489)
                     /locus_tag="PK087_03865"
     CDS             complement(691..>1489)
                     /locus_tag="PK087_03865"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894848.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOH18441.1"
                     /translation="FPVTVRYLDPPLHEFLPTDPEDIKDLAKELNISEEKLNQKIASL
                     HEFNPMMGHRGCRLSVSYPELAEMQTRAVMEAYVNVKKRNPKYEAVPEIMIPLIGDER
                     ELRFVKEIVVRVADEILKQNKMSVDYKVGTMIEIPRAALRSDHIAKEADFFSFGTNDL
                     TQMTFGFSRDDAGSFLPHYYEKRILESDPFARLDQAGVGSLVKLSCDLGRSVKPDLKL
                     GVCGEHGGEPSSVEFFHRVGLDYVSCSPFRVPIARLAAAQAVIKEKK"
BASE COUNT          504 a          267 c          264 g          454 t
ORIGIN      
        1 caataaaagt taagatggaa aactattatg tgacacggta ataaaaccaa tattaaggag
       61 gaaaacatga aaaaattttc gctttttatt ttgctaatta tcgcgaattt tgtattttta
      121 agtgcatcaa atatagatgc ctctggttta gaaaagataa agattgaaga gaagattaat
      181 taccaatcaa ttgaggacga tataattata tttgcaaata tagaggattt aagtttactt
      241 aaagatgcta aagaattaat atcgccagat catatcgtta ttatttataa tcttgaagga
      301 gatgtcgtag atttgctcta caatcaagag tataaaaata catttgctta tatatactat
      361 tataactcct taggaacctt agttaggatg agagtttcag gaacaaactt atcaaaaaac
      421 tcaaggaaga ttgagttaaa aaaacacctt aatttatatt atcaaaaata tgaacttgaa
      481 aaaagagaaa aagagatgtt tatagcaagt tgaaattgac caccccttgt attaaataca
      541 cttatattaa agtcaatgtt aacttggttt taatggggtt gttctaataa tcgtcactag
      601 aattcttgat cggtgccctt atcattaaaa tccttgactt accaaatatt aaaagagctg
      661 ttggaaatta atccgacagc tctttttatt ttactttttt tctttaataa cagcttgagc
      721 agcagccaag cgcgcaattg gaactctaaa cggtgaacaa gatacataat caagcccaac
      781 gcggtggaag aattccactg aagaaggttc gccaccatgt tcgccacaaa cccctaattt
      841 aaggtccggt ttaacactgc gtcctaaatc acaggacaac ttgactaagg aaccgacacc
      901 ggcttgatca aggcgagcaa agggatcact ttcaagaatg cgtttttcat aataatgagg
      961 caggaacgag ccggcatcat cacggctaaa accaaaagtc atttgcgtta ggtcattcgt
     1021 cccgaaactg aagaaatcag cttctttcgc aatatgatcg gaccgtaaag ctgcccgcgg
     1081 aatttcaatc attgttccga ctttataatc aacgctcatt ttgttttgtt ttaagatttc
     1141 atcagccaca cggacaacta tctctttcac aaaccgaagt tcgcgttcat caccaataag
     1201 aggaatcatg atttccggaa ctgcttcata tttaggatta cgtttcttca cattaacata
     1261 ggcttccatc actgctctgg tttgcatttc cgctaattcc ggataggaaa cagaaagacg
     1321 gcagccacga tgtcccatca ttggattgaa ttcatgtaaa ctggcaattt tttggtttag
     1381 tttttcttca gaaatattta attctttcgc taagtcttta atatcttccg gatcagtcgg
     1441 taagaattca tgtaacggcg gatcaagata gcgaactgtg accgggaag
//
LOCUS       DAOSGN010000257         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_89653_length_1482_cov_1.864751, whole genome shotgun sequence.
ACCESSION   DAOSGN010000257 DAOSGN010000000
VERSION     DAOSGN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89653_length_1482_cov_1.864751"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PK087_03870"
     CDS             complement(<1..605)
                     /locus_tag="PK087_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH18442.1"
                     /translation="MKLKLIFLFALLMVLMGCQAPRLEISIDGPTTIFEGETIQYAIT
                     FNPATTQKKLNWESSNPEVATVNENGQLIALVAGEIRLFARLQDNRQVYAYIDVTIKP
                     LTMTIVGPREISAYESVSYTITFNPDSLQKTVNWESSNPKIATVNENGQLTGLKAGQV
                     RLVARLVDNSNVNTYIDVTIKPLVIEEIMITGKTTLAWKES"
     gene            complement(615..>1482)
                     /locus_tag="PK087_03875"
     CDS             complement(615..>1482)
                     /locus_tag="PK087_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18443.1"
                     /translation="LDDFIDRLIENHGFHPGTLINIINQTLSDYICFHTLYNRKPLLT
                     AATKQKMKAILVDEFYELEELKGILEKQGVQLQDYQYFSNLWLDEFGYKTHDVNYVIK
                     KTYSSIKTLFFEKVLSHDEYVITPKDRQMRETTLILFIETLRFSYSMFLIDNERLISM
                     KKLAKQGVSEQSLRNYVAALAEYLPHDEYFTYQSLVNEQYYLHHPAFQIVEDYHLHPD
                     IIIGLIRNVKGIKKTTKGNLFKISSQPSIIGEFLKRLQKQHQFADVDQLQQFVKDHYG
                     LVVKKTSFSTNK"
BASE COUNT          448 a          259 c          283 g          492 t
ORIGIN      
        1 aaactttctt tccacgccaa ggtggttttt cccgtaatca ttatttcttc aatcacgagc
       61 ggtttaatcg tgacatcgat ataagtatta acattgctgt tatcaacaag ccgagccact
      121 aaccgcactt gcccggcttt tagaccggtt aattgtccgt tttcattaac ggtggctatt
      181 ttcggattac ttgactccca attaactgtt ttctgaaggg agtcgggatt aaaggtgatg
      241 gtatacgaaa cgctttcata tgccgatatt tccctaggac cgacgatagt catcgtcagg
      301 ggtttaatcg tcacatcaat atacgcataa acttggcggt tgtcctgaag ccgggcaaat
      361 aagcgtattt ccccggctac taaagcgatt aattgcccgt tttcattaac ggtggctact
      421 tccggattac ttgattccca attaagtttt ttttgggtag tggccggatt aaaggtgatg
      481 gcatattgaa tggtttcgcc ctcgaaaatg gtcgtgggac cgtcaatgct tatttcaagc
      541 cttggcgctt gacagcccat taataccatt aatagggcaa aaagaaaaat caattttagt
      601 ttcataatat cctcttattt gttagtacta aaagatgttt ttttgacaac caatccataa
      661 tgatccttaa caaattgttg taattgatcg acatcagcaa attggtgttg tttttgtaag
      721 cgtttaagga attccccaat gattgatggt tgggaagaaa tcttaaacaa attgcctttg
      781 gtggtctttt tgatgccctt gacattacgg attaagccga tgatgatatc ggggtgaaga
      841 tgataatctt ccacaatctg aaaagccgga tgatgtaaat aatattgttc attgactaaa
      901 ctttgatagg taaaatattc atcatgcgga agatattccg caagagcggc aacataattg
      961 cgtaaacttt gttcggaaac tccttgtttt gctaatttct tcatcgaaat taatcgttca
     1021 ttgtcaatca aaaacatgga ataagaaaat ctcaaggttt caataaataa tattaaagtt
     1081 gtttcccgca tttgacggtc tttaggagta atcacatatt catcatggga aaggactttt
     1141 tcaaaaaaga gcgttttgat ggatgaataa gtttttttga taacataatt gacatcatga
     1201 gttttataac caaattcatc caaccataag ttagaaaaat attgataatc ttgaagttga
     1261 acaccttgct tttcaagaat gccttttaac tcctctaatt cgtaaaactc atcaacgagg
     1321 atggctttca ttttttgttt tgtagcggcc gttaaaagtg gtttacggtt atataaagta
     1381 tggaaacaaa tataatcact taaggtttgg ttgatgatgt taataagcgt tcccggatga
     1441 aaaccatgat tttcaattaa gcgatcgata aaatcatcta aa
//
LOCUS       DAOSGN010000258         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_89764_length_1480_cov_4.327018, whole genome shotgun sequence.
ACCESSION   DAOSGN010000258 DAOSGN010000000
VERSION     DAOSGN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89764_length_1480_cov_4.327018"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PK087_03880"
     CDS             <1..631
                     /locus_tag="PK087_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOH18444.1"
                     /translation="NARFKAKFFARRGVGYVGAEENKVFCKDKNGNTIISRIATDAIY
                     TPVTKVRYDVEKTRVDENVDYEKLTMEVWTNKSITAANAASLASKFLIDHFVVVARLN
                     EIVIEQDYMYEREEKITNRKLEKKIEELDLSVRSYNCLKRAGINTVGELAQKAEEEMM
                     RVRNLGRKSLKEVVQKLREIGLDLRRSYDSDYDYSDFDDDSDDSSDDLN"
     gene            658..1026
                     /gene="rplQ"
                     /locus_tag="PK087_03885"
     CDS             658..1026
                     /gene="rplQ"
                     /locus_tag="PK087_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOH18445.1"
                     /translation="MALGYRRLGRNSAQRKALLRDLVTDLIINEKIETTYHKAKELQK
                     LADKMVTLGKANTLSSRVKAAQMVRFEFVDEKTEQYAIQKLFSDIAVRYKDRKGGYTR
                     VLKLMPRLGDSAPMAIIEFV"
     gene            1184..>1480
                     /locus_tag="PK087_03890"
     CDS             1184..>1480
                     /locus_tag="PK087_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18446.1"
                     /translation="MKKFDIIVISIVFLFAAFLYFMYFSSFGRPDNLTVEVYYRNMVI
                     YSIDIDEDTDVTVEITADDYLLTVKIGDLTKTYDITSDKHLINTIHITKPLVEMT"
BASE COUNT          493 a          239 c          306 g          442 t
ORIGIN      
        1 aaatgctcgt tttaaggcta agttttttgc tcgtcggggc gttggttatg ttggtgccga
       61 agaaaacaaa gttttctgca aagacaaaaa cggtaacacc atcatcagtc gcatcgccac
      121 ggatgccatt tatactccgg tgacgaaagt aagatacgat gtggaaaaaa cccgggttga
      181 tgaaaatgtt gattatgaaa aactaaccat ggaagtgtgg actaataagt ccattaccgc
      241 tgctaatgcc gcatcattag cctcgaagtt cttaatcgat cattttgtag tggtagcaag
      301 attaaatgaa attgtcattg aacaagatta tatgtacgaa cgcgaagaaa aaatcacgaa
      361 caggaaattg gaaaagaaga ttgaggagtt ggatttatcg gttcgttctt ataactgctt
      421 aaagcgcgct ggcatcaata ccgttggtga gttagcccaa aaggcggaag aagaaatgat
      481 gcgagtacgt aatttaggtc gtaaatcact gaaggaagtt gtacaaaaac tacgagaaat
      541 tggtcttgat ttacgccgtt catatgattc ggattacgat tattcagatt ttgatgatga
      601 ttcagatgat tcgtcagatg accttaacta aaactattcc tgaaggagga aactaatatg
      661 gcattaggat atcgtcgatt aggacgtaat tccgcccaac gcaaagcgtt gttgcgtgat
      721 ttggttacgg atttaatcat taacgaaaag attgaaacaa catatcataa agccaaggaa
      781 cttcaaaagt tagcggataa aatggtgaca cttggtaaag ccaatacttt atcatcccgt
      841 gtcaaagcag cgcagatggt tcgtttcgaa tttgttgacg agaaaaccga acaatatgca
      901 attcaaaaac tattttccga tatcgctgtg agatataaag accgcaaagg cggatacacc
      961 cgcgttttaa aacttatgcc gcgtcttggt gattctgctc cgatggctat tattgaattt
     1021 gtatgagaaa aacccctgaa aaggggtttt ttgtttggaa gaaattaata tttttaatta
     1081 ataattttta gccaaaacaa tttaacacta tcctatatta gcgattgact tgtaaaaaga
     1141 ggaaaaattt ggtaaaatat gacattgtaa aaggtgataa aatatgaaaa aattcgacat
     1201 catcgttatt tccattgttt ttttgtttgc cgctttttta tattttatgt atttcagttc
     1261 ctttgggcgt cccgataatt taacagtaga agtttattac cgcaacatgg tgatttattc
     1321 catcgatatt gatgaagata cggatgtgac ggtggaaatc acagcggatg attatctatt
     1381 gacagtaaaa atcggtgatt taacgaaaac ttatgatatt acttctgata agcatctcat
     1441 taatactatc cacatcacca aaccgcttgt ggaaatgaca
//
LOCUS       DAOSGN010000259         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_90084_length_1477_cov_2.193390, whole genome shotgun sequence.
ACCESSION   DAOSGN010000259 DAOSGN010000000
VERSION     DAOSGN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90084_length_1477_cov_2.193390"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..820
                     /locus_tag="PK087_03895"
     CDS             293..820
                     /locus_tag="PK087_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOH18447.1"
                     /translation="MEINRQFKELMQDYIISEQNEEIEEVLVALLRKRNLTIAFGESC
                     TGGMLAAKLINVPHASDVIKESFVVYSNEAKIKYLHVNEATITQYGAVSEQTIEQMLN
                     GLFLETKAQVCVTVSGIAGPSGGSSEKPVGLVYYGIKINNQQIIEHRHFKGNREMVRQ
                     RTTLFVLFRLWQLLK"
     gene            890..>1477
                     /locus_tag="PK087_03900"
     CDS             890..>1477
                     /locus_tag="PK087_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOH18448.1"
                     /translation="MEEKNYINEQLLNKIATEQNVKINQIQAVLKLIEEGGTVPFIAR
                     YRKEVTGGLDEEQIRAIFQEWEYGQKLAERKEDIMRLIDEKGKLTEELKQEIIKCNKL
                     AELEDIYRPYKEKKKTRATDAKQKGLEPLALYLLSFPTEGDVLLEAEKYVTKEVTEEL
                     EKNNLVVKNAKEALQGAMDIIAEIVSDEPKYRRWIR"
BASE COUNT          523 a          216 c          323 g          415 t
ORIGIN      
        1 cggtcgatgg tgcgattatt gaataagatg ggaagattta tattgtctta gtcggaccgt
       61 cttatgaaat gaatcccatg gtcattgatt cggttattcc ttatttaaaa actaaaacca
      121 atatggtaat ccaagcaaaa gattatctcg tgatgggaat ggtgaatctt tttttgaaga
      181 gttattaaag cctctcatta gtcaaacccc ctatgtcagt atttcaccct atgccagttt
      241 aggaaagatt agataccaaa ttgttgggac gggtaatttt caagaagctt ttttggaaat
      301 aaaccgccaa tttaaagagc taatgcagga ttatatcatt agtgaacaaa atgaggaaat
      361 tgaagaagtc cttgttgcac ttttaagaaa gcgcaacttg accatcgctt ttggtgaaag
      421 ttgtaccggg ggcatgttag cagcaaaatt aattaatgtt ccccatgctt ccgatgtaat
      481 caaagaaagt tttgttgttt atagtaatga agccaaaatt aaatacttac atgtcaatga
      541 agcaaccatc actcaatatg gtgctgtcag tgaacaaacg attgaacaga tgttgaacgg
      601 tttattttta gaaacgaagg cccaagtttg tgtaactgtg agcggtattg ccggaccgag
      661 tggtggaagt agtgaaaaac cggtggggtt ggtatattat ggcatcaaaa tcaacaatca
      721 acaaattatc gaacatcggc acttcaaagg caatcgggaa atggtaaggc aaagaacaac
      781 gctttttgtt ttattccgat tatggcagtt gctaaaataa aaaaaagttg ccttgaaagg
      841 ccaaaaatgg tataataatt atgttgtaaa tttgatggag gatataaaaa tggaagaaaa
      901 aaattatatc aatgagcaat tattaaataa aattgcgacg gaacaaaatg tcaagataaa
      961 ccaaattcaa gccgttttaa agctgattga agaaggtggg accgttcctt tcattgctcg
     1021 ttatcggaaa gaagtaaccg gcggccttga tgaagaacaa atccgcgcta tttttcaaga
     1081 atgggaatat gggcaaaaat tagctgagcg caaagaagat atcatgcgct tgattgatga
     1141 aaaaggaaag ttgacggaag aattaaaaca agaaattatt aaatgtaata aattagcgga
     1201 attagaagat atttatcggc cttataagga gaaaaagaaa acccgagcga ctgatgctaa
     1261 acaaaaaggt cttgaacctt tggcacttta tttgttaagt tttcctactg aaggcgatgt
     1321 tttgctggaa gccgaaaaat atgttactaa agaagtaacg gaagagctag agaaaaataa
     1381 tttggttgtg aaaaacgcta aggaagctct tcaaggagca atggatatta ttgccgagat
     1441 tgtttctgat gagcccaaat atcggcgctg gattcgc
//
LOCUS       DAOSGN010000260         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_90320_length_1474_cov_3.424242, whole genome shotgun sequence.
ACCESSION   DAOSGN010000260 DAOSGN010000000
VERSION     DAOSGN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90320_length_1474_cov_3.424242"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1474
                     /locus_tag="PK087_03905"
     CDS             <1..>1474
                     /locus_tag="PK087_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOH18449.1"
                     /translation="DWTIMQAADSANIEIPRLCFVKDINETSACRICVVEIEGIKSLK
                     NSCTVKVADGMKVKTNTERVKKAVRNNLELLAANHHFECWRCPREHNCEFLHLLRRYN
                     VPNEMGEDPFHERKAVISNITDALVLDSSKCILCGRCISACEKLAGTAVLNFNERGFV
                     TFVGPAMNHPLDDAGCIYCGKCIQYCPTGALKEKDDVDLVLDLLEKGEHFVVVQTAPA
                     VRAALGEEFNYEIGTNVEGKMFRALHDLGFNDITDTNFAADVTILEEGHEFVERLKKH
                     LANEPTPLPMFTSCSPGWIRYIEIYYPEFLDHLSTCKSPQQMQGALIKHYYAKKLNIP
                     VEKIRVVSIMPCIAKKAEAKLPQMEVDGIRDVDYVLTTRELARMIKRRGIDFHKLEPL
                     TPSSPMASYTGAGAIFGATGGVMEAALRTVKEQLENKPFDLTALAGLRGVDDGIKEVE
                     VEVTGLKLTVAVVHGTKNVPEMLRRVKENPGKYVFIEIMAC"
BASE COUNT          437 a          271 c          362 g          404 t
ORIGIN      
        1 gattggacga ttatgcaagc agcggattca gcaaatatcg aaattccgcg gctgtgtttt
       61 gtaaaggaca tcaacgaaac atcggcatgc cggatttgcg ttgtggaaat cgaaggcatt
      121 aaaagtttaa agaactcttg tacagtcaaa gtggctgacg gtatgaaggt gaaaaccaat
      181 accgaacgag tcaaaaaagc tgttcgcaat aatttggagt tattagcggc gaaccatcat
      241 tttgaatgtt ggcgctgtcc gcgtgaacat aattgtgagt ttttgcattt attaagacgc
      301 tataatgttc ctaatgaaat gggcgaagat cctttccatg agcgaaaagc ggtcattagc
      361 aatatcaccg acgctttagt tcttgacagt tccaaatgta ttttatgcgg tcgttgtatt
      421 tcggcttgtg aaaaactggc cggaacagcg gtgttaaatt ttaacgaacg cggttttgta
      481 acctttgtgg gaccggcgat gaatcatccc ctcgatgatg cgggctgtat ttactgtggg
      541 aaatgcattc aatattgccc aaccggcgct cttaaagaaa aagacgatgt tgatttagtg
      601 cttgaccttt tggaaaaagg ggaacatttt gtagttgttc agacggcacc ggctgttcgg
      661 gctgctttag gtgaagaatt caactacgaa attggcacga atgtggaagg caaaatgttt
      721 agagctttgc acgatttagg gttcaatgat ataactgata ccaactttgc ggcggacgta
      781 accatccttg aagaaggtca cgaatttgtt gagcgtctaa agaaacattt agcgaatgaa
      841 ccaacacctt taccgatgtt tacatcttgt tcaccgggat ggattcggta tattgaaatt
      901 tattatccgg aattccttga ccatttatca acatgtaaat cgccacaaca aatgcaagga
      961 gcattaatca aacattacta tgcgaagaaa ctgaacattc cggttgagaa aattcgcgtg
     1021 gtttccatta tgccgtgcat tgccaagaaa gcggaagcta aattgccaca aatggaagtt
     1081 gatggcattc gcgatgttga ttatgtttta acaaccaggg aattagcgcg aatgattaaa
     1141 cgtcgcggca tcgatttcca caaacttgaa ccattgactc caagcagccc catggcttct
     1201 tacaccggag ccggtgctat ttttggagca acgggtggcg tgatggaagc agctttaaga
     1261 acggtcaaag agcaattgga aaacaaacct tttgacttaa cggcacttgc cgggttgcgg
     1321 ggagttgatg acggcattaa agaagtggaa gtggaagtaa ccggcttgaa attaacggtt
     1381 gccgttgtcc atggaacgaa aaatgttcct gaaatgctaa ggcgagttaa ggaaaatcct
     1441 ggcaagtatg tgtttattga aatcatggct tgta
//
LOCUS       DAOSGN010000261         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_90416_length_1473_cov_3.506347, whole genome shotgun sequence.
ACCESSION   DAOSGN010000261 DAOSGN010000000
VERSION     DAOSGN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90416_length_1473_cov_3.506347"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PK087_03910"
     CDS             complement(<1..456)
                     /locus_tag="PK087_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18450.1"
                     /translation="MKKITKLLFVFTSILLAFGTSLSLKAQNIPNWEFLPLGYNYLDP
                     DNFNVSGSYSFFEVSNFNSFRVKPNTEYTLFQTFPTETFFGNHQVTAYDANSVLITSD
                     FVFEYTHYDGNYSTITFTTPANTKYLDIDFEIIYDGSEPCDFYPLDEYIV"
     gene            complement(569..>1473)
                     /locus_tag="PK087_03915"
     CDS             complement(569..>1473)
                     /locus_tag="PK087_03915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOH18451.1"
                     /translation="TNYIREVVTQQNNDPTRAVLEIIPAVNAKSYVHIDDMYWRCYRF
                     VTGAKTYEIVENPELFFEVGKAVGTFQKQLMDFPIEKLSITIPNFHNTPARFHRFKEV
                     VNEDPKRRALEVFNEIKFLYNREEYVNNITKHLETGEIKTRVTHNDTKLNNIMIDERS
                     NEAICVIDLDTVMPGSVLYDFGDAIRVGASTALEDEKDLSKVKINHVLFEAFSQGFLE
                     KTHDILTEKEIENLVESARIITLECGMRFLTDYLEGDNYFSIHYETHNLDRARTQFKL
                     VEEIENDYEDLEEIIQNILKDLRK"
BASE COUNT          505 a          271 c          241 g          456 t
ORIGIN      
        1 aacaatatat tcatccagcg gataaaaatc acagggctcc gaaccatcat aaataatctc
       61 aaaatcgatg tctaaatatt tagtattggc cggagtcgta aaggtaatgg tcgagtaatt
      121 accgtcataa tgcgtgtatt cgaaaacaaa atcggaagta ataagaacgg agttggcgtc
      181 ataagcagtt acttgatgat tcccgaaaaa agtttccgta ggaaaggttt gaaacaaagt
      241 gtattccgta ttaggtttaa ctcggaaaga attgaagtta gatacttcaa agaaggaata
      301 cgacccactg acattaaaat tatcgggatc taaataatta tagccgagcg gtaaaaattc
      361 ccaattagga atgttttgag ctttgaggga caaacttgtc ccaaaagcca ataaaatgct
      421 tgtaaaaaca aataatagtt ttgtaatttt tttcattatt ttctcctttt cgaaaataat
      481 tcaaaaaatt aaacacgcca ataaaattat aacaaaaagc aagacgaaaa caacgagaaa
      541 atttattttt caaacaagca aaaaagactt acttacgcaa gtctttcagg atattttgaa
      601 ttatttcttc taaatcctca taatcatttt caatctcttc gactagtttg aattgtgtcc
      661 gcgctcgatc caaattatga gtttcataat ggatgctgaa ataattatcg ccttctaaat
      721 aatccgtcaa aaagcgcatt ccacactcta aagtgataat tcgcgccgat tcaactaaat
      781 tttcaatctc tttttcagtt aggatatcat gagttttctc taaaaaacct tggctgaaag
      841 cttcaaataa aacatgatta atttttactt tacttaaatc tttttcgtcc tcaagggccg
      901 tcgatgcccc gacacgaata gcatcaccaa aatcatataa caccgagccc ggcataacag
      961 tatccaaatc aattacacaa atggcttcat tgcttctttc atcaatcatg atgttattta
     1021 atttggtatc attatgagta acacgggttt tgatttcccc cgtttctaag tgcttagtaa
     1081 tgttattgac atattcttca cggttgtaaa gaaatttaat ttcattgaac acttccaaag
     1141 ctcttcgttt cggatcttca ttaacaactt ctttaaaacg atggaaacga gccggggtgt
     1201 tgtggaaatt gggaattgtg attgacaatt tttcaatcgg aaaatccatt aattgctttt
     1261 gaaaggtccc aacggctttg ccaacctcga aaaataattc cgggttttcc acaatttcat
     1321 aagttttagc tcccgtcaca aaacgataac aacgccaata catatcatca atgtgaacat
     1381 aggatttcgc attaacagca ggaatgattt ccaagacagc acgggtggga tcattatttt
     1441 gttgggtgac gacttcccga atataattgg taa
//
LOCUS       DAOSGN010000262         1470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_90721_length_1470_cov_2.602827, whole genome shotgun sequence.
ACCESSION   DAOSGN010000262 DAOSGN010000000
VERSION     DAOSGN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90721_length_1470_cov_2.602827"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK087_03920"
     CDS             complement(<1..207)
                     /locus_tag="PK087_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18452.1"
                     /translation="MKSYTVKISILFSAILIIVALVVYGAYYFIADHFIEGKAAENML
                     YINQKIALNVDLKINYDYDKLTDVI"
     gene            351..424
                     /locus_tag="PK087_03925"
     tRNA            351..424
                     /locus_tag="PK087_03925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Thr,seq:ggt)
     gene            complement(468..>1470)
                     /locus_tag="PK087_03930"
     CDS             complement(468..>1470)
                     /locus_tag="PK087_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOH18453.1"
                     /translation="AFSIGNELKLSYFKKVGTRPIVIAIFESLFAVLFVFGGLIVFFA
                     IKGTLDLTNIRFSLVLSAIAAATAPAATLMVVRQYWASGPLTEMLMSVVALDDSVALM
                     LFGINVAIANGLGGNGDSSLFIQIIKPIWEIIFSLGIGAFFGIVLNFACKWFSNRGNR
                     ISLTLAFVFLTIFLTDYLGGSVLLACMAFGGLFANISKDYDEVNGLTHYITPPIFMMF
                     FVLSGAELKLSILKIVGIIGLVYIITRVAGKIFGAWFGSKVTHADPKIAKYLGFTLIP
                     QAGVAIGLSLIATQILNPEMGSQIRTIVLAATLVYELVGPIITKITLKKAQEIPNLA"
BASE COUNT          506 a          310 c          271 g          383 t
ORIGIN      
        1 aataacatca gttaatttat cataatcata attgatcttt aaatcgacat taagggcaat
       61 cttttgatta atgtaaagca tgttttccgc cgctttacct tcgataaaat gatcggcaat
      121 gaaataataa gctccataaa cgaccaaagc gacgattatt aagatggcag agaacaaaat
      181 cgatatttta acggtataag acttcatcgc ttggctcctc tttaactaaa ttaacttaat
      241 tatacactaa aagttgctaa ttgcaaggta tctttcatca agaatgatag tttccgttac
      301 aaaaaggttg aaatcttcaa tcttttttgt tataatacta tctagaaaaa gctcctatag
      361 tttagtggta aaacaaagca atggtaatgc ttagatccaa gttcgattct tagtaggagc
      421 accattgact aatttaaaca accccttttg ataggggttg ttttttttta ggctaaatta
      481 ggtatttcct gggccttttt caaagttatt ttggtaataa tcggaccaac cagttcataa
      541 accaaggttg ctgccaacac aatcgtgcgg atttgggaac ccatctcagg attaagaatt
      601 tgggtcgcaa tgaggcttaa accgatggcc accccggctt ggggaatcaa agtaaaacct
      661 aagtatttcg caatcttagg atccgcatgg gttactttgc tgccaaacca agccccgaag
      721 attttaccgg caacccgggt gatgatataa acaagaccaa taataccgac aatcttgagg
      781 attgatagct ttaattccgc cccacttaaa acaaaaaaca tcatgaaaat cggtggggta
      841 atataatggg ttagtccgtt cacctcatca taatccttgc tgatgttggc aaaaagaccg
      901 ccaaaggcca tgcaggccaa caaaaccgaa ccgcctaaat aatcagttaa aaagatagtt
      961 aaaaagacaa aggctaaagt tagagaaatc cgattaccgc gattggaaaa ccacttacaa
     1021 gcaaagttta acacaatccc aaaaaaggcg ccaatgccca aagaaaagat gatttcccaa
     1081 atcggtttaa tgatttgaat gaacagggaa ctatcaccat tgccccctaa accgttggca
     1141 atcgccacat tgatgccaaa cagcattaag gccaccgaat catcaagggc cacgacgctc
     1201 attagcattt ccgtaagcgg tccggaagcc cagtattgcc tcaccaccat taaggttgcg
     1261 gccggtgcgg tcgccgcagc aatcgccgat aacaccaagg aaaagcgaat attcgttaaa
     1321 tcgagcgttc ctttaatagc aaagaaaaca attaagccgc caaaaacaaa caacacggca
     1381 aataatgatt caaagatggc aattacaatc ggtctcgtcc cgactttttt aaaatagctg
     1441 agctttaatt cattgccaat ggaaaaggca
//
LOCUS       DAOSGN010000263         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_90973_length_1467_cov_3.184136, whole genome shotgun sequence.
ACCESSION   DAOSGN010000263 DAOSGN010000000
VERSION     DAOSGN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90973_length_1467_cov_3.184136"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PK087_03935"
     CDS             complement(<1..191)
                     /locus_tag="PK087_03935"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387652.1"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HOH18454.1"
                     /translation="MNNWFTEFWTNHIKFSIRIKEHLFSQQTPFQKLDFYDSYELGRF
                     FTLDDIMMANEKDEFIYHE"
     gene            complement(188..994)
                     /gene="speD"
                     /locus_tag="PK087_03940"
     CDS             complement(188..994)
                     /gene="speD"
                     /locus_tag="PK087_03940"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOH18455.1"
                     /translation="MVRKQHPIKLYGFNNLTKTLSFNIYDICYTGDEFERKAYIEYIN
                     QQYNSNRLKKILTEVTKIINAKILNIATQDYEPQGASVTVLVSEYHDVNGLNEVPKKD
                     SVVGHLDKSHLTVHTYPESHPGNPISTFRVDIDVSTCGEISPLKALHYLISQFDSDII
                     TIDYRIRGFTRDLNGEKHFLDHSIDSIQDYIPDEILSKYTTYDVNLAYTNIFHTKMML
                     NTLVLDNYLFNLNSADLTEEKVAKIRQAINSEMAEIYYGMNIPRMKLEKK"
     gene            complement(1278..>1467)
                     /locus_tag="PK087_03945"
     CDS             complement(1278..>1467)
                     /locus_tag="PK087_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18456.1"
                     /translation="STTAFDFAFLWLNYKDTSIKEITVKLPSDETQAQKLKNSLLQQI
                     ESDNVIRYLGNDFVINFE"
BASE COUNT          486 a          248 c          225 g          508 t
ORIGIN      
        1 atttcatgat aaataaactc atctttttca ttggccatca taatatcatc taaagtaaaa
       61 aaccgaccca attcataaga atcataaaaa tcaagttttt gaaacggggt ttgttgtgaa
      121 aagagatgtt ctttgatgcg aatagaaaat ttaatatgat tagtccaaaa ttcagtaaac
      181 caattattca ttttttctcc aatttcatcc ggggaatatt catcccataa tatatttccg
      241 ccatttcgga attaatcgcc tgcctgattt tcgcaacctt ttcttcggtt aaatcggcac
      301 tgttaaggtt aaataagtaa ttatccagca ccaaagtatt caacatcatt ttggtgtgaa
      361 agatattagt ataagctaaa ttgacatcat aagttgtgta tttcgataaa atttcgtccg
      421 gaatatagtc ttgaatcgaa tcaatactat gatcaaggaa atgtttctca ccatttaaat
      481 cacgggtaaa acctctaatc cgataatcaa tggtaatgat gtcggaatca aattgactga
      541 ttaaataatg taaagccttc aatggcgaaa tttccccgca tgtggaaaca tcaatgtcca
      601 cccgaaaagt gctgatggga ttcccgggat gactttccgg ataagtgtga accgttaaat
      661 ggcttttatc caaatgacca acgacggagt cttttttagg aacttcattt aatccattca
      721 catcgtgata ttcagatact aaaacggtaa cgctggcccc ttgtggttca taatcctgcg
      781 tcgcaatatt taaaattttc gcattaatta ttttcgttac ttcagttaaa atctttttta
      841 aacgattgct gttatattgt tggttaatat attcaatata agcttttctt tcaaattcat
      901 ccccggtata acaaatatca taaatattaa aactaagggt cttagttagg ttgttgaaac
      961 catagagttt aatcgggtgc tgtttgcgaa ccatgggaaa cctcctggaa agaaatgatt
     1021 gttcatatga actacttatc atattatatt ataatcatgg tcatttttca accttaataa
     1081 taattgtttt tcttaaagat cttttcgaca tctcgatacc ataaatcatc aaaaagaagc
     1141 ttcaattttt gacttgagat ctttgtttct aaaaagaaaa agctttggtc ccaaagttat
     1201 actttgacca aagctaagga acaattgtat agcgaagtta ttgttagtga ataaatgccc
     1261 attaaatatt gtttttgcta ttcaaaatta atgacaaaat catttcctaa atagcgaata
     1321 acattatccg attcaatttg ttggagcaaa ctatttttca atttctgggc ttgtgtttca
     1381 tcacttggca gtttaaccgt aatctctttg attgatgtat ccttataatt caaccaaaga
     1441 aacgcaaaat caaatgctgt tgttgaa
//
LOCUS       DAOSGN010000264         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_90980_length_1467_cov_2.975212, whole genome shotgun sequence.
ACCESSION   DAOSGN010000264 DAOSGN010000000
VERSION     DAOSGN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90980_length_1467_cov_2.975212"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PK087_03950"
     CDS             <1..266
                     /locus_tag="PK087_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOH18457.1"
                     /translation="IAMYNEHYPMIEYLLKKKANVNEIFYGRSYLYRAILRNNYEIAK
                     LLVDYGADVNYIDNRHRTIFSYAIDYASDEIIDLLVTKKASFV"
     gene            288..875
                     /gene="rdgB"
                     /locus_tag="PK087_03955"
     CDS             288..875
                     /gene="rdgB"
                     /locus_tag="PK087_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009410674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOH18458.1"
                     /translation="MKILFASHNLHKLKEIQSLLPSFELLSLKELHDNDDVEEDGNTF
                     AANAFKKAHYYFQKYHLPVFADDSGLIVSALNGEPGVKSARYSGLDADYHRNNLLLLK
                     NMEGIKERTAFFVTVICFIEESGQVHFFEGKWKGIIHEQLQGDNGFGYDPLFYLPELD
                     KTAAELTIMEKNQFSHRALAFQQFSRFLKKHYPCF"
     gene            973..1158
                     /gene="rpsU"
                     /locus_tag="PK087_03960"
     CDS             973..1158
                     /gene="rpsU"
                     /locus_tag="PK087_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOH18459.1"
                     /translation="MAKTIVRENETIEDAIKRFKRDVSRNGVLAETRKRQYFLKQSEI
                     RKEKKRAARKNAYRRSN"
     gene            1247..>1467
                     /locus_tag="PK087_03965"
     CDS             1247..>1467
                     /locus_tag="PK087_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18460.1"
                     /translation="MSEYQTIYHFKNANNEAKILGPNNQYWRMLEDYYQTEVLIRHSE
                     VKIALNHPLSALLERYFNVVETILENKVP"
BASE COUNT          482 a          233 c          283 g          469 t
ORIGIN      
        1 taattgccat gtataatgaa cactacccga tgattgaata tttgttaaag aaaaaagcca
       61 atgttaatga aattttttat ggccgcagtt atttgtatcg agcgattttg cggaataatt
      121 atgagatcgc caaattgtta gtggattatg gtgcagatgt caattacatt gataatcgtc
      181 atcgaaccat tttttcgtat gcaattgatt atgccagcga tgaaatcatt gatttattgg
      241 taaccaaaaa agcttcattt gtatgaagct ttttctgaag gtgtcctatg aaaattcttt
      301 ttgcttcaca caatcttcat aaattaaaag aaatccaaag tttacttccg tcttttgaat
      361 tgctgtcttt aaaagaacta catgataatg atgatgtgga agaagatggc aatacatttg
      421 ccgcaaacgc ttttaaaaaa gcgcattatt attttcaaaa gtaccatctc ccggtttttg
      481 ccgatgattc cggtttaatt gtttccgctt taaatggtga accgggtgta aaatcggctc
      541 gttattcggg cttggatgct gactatcatc gcaacaattt gttattgctt aaaaatatgg
      601 aaggaataaa ggagcgaacg gctttttttg tgacggtgat atgctttatc gaagagtccg
      661 gtcaagtgca cttttttgaa ggtaagtgga agggaataat ccacgaacaa cttcaaggcg
      721 ataatggctt tggctatgat ccgctttttt acttgccgga attggataaa acggcggctg
      781 aattaacgat tatggaaaaa aatcagtttt cacatcgcgc tttggcgttt caacaatttt
      841 ctcgattctt aaaaaaacat tatccatgct tttaaaggtc gattagggta aaaaaatatt
      901 tttctttaca attgtaatag tttatagtat aatattgtag gaattattcg tgtggaagga
      961 gggacgagaa caatggcaaa aacgatagtc cgtgaaaatg aaacaattga agatgcaatc
     1021 aagcgcttta aacgcgatgt gtcaagaaat ggcgtccttg cggaaacacg caaacgacaa
     1081 tattttttga agcaaagcga aattcgtaaa gaaaaaaagc gtgcggctcg taagaatgct
     1141 tatcggcgtt caaactaaga aaagccttcg ggcttttttt tgttgcgatt atttacttaa
     1201 caattagtta tatttatgtt acaattaacg aaaaaggaag gaataaatgt cggaatatca
     1261 aaccatttac cattttaaaa atgccaacaa tgaggctaaa atcctcggac ctaataatca
     1321 atattggcgc atgcttgagg attattacca aaccgaagtt ttaattcgtc attcggaagt
     1381 aaaaatagcc cttaatcatc ccttatccgc tcttttagaa cgctacttta atgttgttga
     1441 aacaatttta gaaaataaag tcccctt
//
LOCUS       DAOSGN010000265         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_90996_length_1467_cov_2.618272, whole genome shotgun sequence.
ACCESSION   DAOSGN010000265 DAOSGN010000000
VERSION     DAOSGN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90996_length_1467_cov_2.618272"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="PK087_03970"
     CDS             complement(<1..1417)
                     /locus_tag="PK087_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOH18461.1"
                     /translation="MILDVFEAYRLNAVIFQVRPTNDAFYSSTINPWSRFLINVEGQY
                     PGWDPMAWMIERCHERNMEFHAWLNPYRASLGVLPDTGDYQSELNSYLQTLSPLNFAR
                     KHPDLLVRGIHAPGDARILLNPAEPLVRKHIYDTIEEIVRKYNVDAIHFDDYFYNGVA
                     DAEDNANYQAYLKNNPYLSKGDWRREQVNILIKGIHDLLENLNSSLNKQVQFGISPAG
                     VWAPADNMGCGAYGQPGGMTDIACYSYSSYVDLFADTKKWVNEGWIDYLVPQNYGNLN
                     GNHPAITRWWANEVSKGDVKLYIGLAPYQYINSGWSIPEMDNQMMLGYSYPSVSGYVF
                     FSIRILRHPTNNNLTKALEKLQERWSHGALPPLVFPKAVSDYSKPTLSAWRNANHINL
                     SFDANPNAYGYLLYRLMKDEVFNAQTTPIFDVVANPKKAFTVRHQADNLTDYTYYVQT
                     VFNDGTMSTDYQVLSTGIYDNN"
BASE COUNT          459 a          322 c          284 g          402 t
ORIGIN      
        1 cattgttatc ataaatcccc gtggaaagca cttgataatc cgtgctcatc gttccatcat
       61 tgaagacggt ttgcacataa taagtataat cagtgagatt atccgcttga tgacgcaccg
      121 taaaagcttt tttagggtta gcaacaacat cgaaaattgg ggttgtttgc gcattaaaaa
      181 cttcatcctt cataagtcga taaagaagat atccataagc attcggattg gcatcaaaag
      241 ataaattaat gtgattggca ttgcgccaag cagacaaagt cggcttggaa taatcagaca
      301 ccgctttcgg aaaaacaagg ggcggtaggg ctccatgcga ccatcgttct tgcaattttt
      361 ccaaagcttt tgttaaattg ttattcgttg gatggcgcaa aattctaatt gagaaaaaca
      421 catagccact aacggacgga taggaatagc ccagcatcat ttggttgtcc atttctggaa
      481 tcgaccaacc tgaattaata tattgataag gcgctaagcc gatgtacagt ttcacatcac
      541 ctttgctgac ttcattggcc caccaccgcg tgatggctgg atgattacca tttaaattac
      601 catagttttg gggaacaaga taatcaatcc aaccttcatt aacccatttt ttcgtgtccg
      661 caaataaatc cacataagaa ctataactat aacaggcgat gtcggtcatg ccccccggtt
      721 gtccgtaagc cccgcaaccc atattatcgg ccggagccca aactccagcc ggactgatac
      781 cgaactgaac ctgtttgttt aaggaggaat tcaagttttc taataaatcg tgaattcctt
      841 taattaaaat attaacctgt tcacgacgcc agtcaccttt tgataaataa ggattattct
      901 ttagataggc ttgataatta gcattatctt ccgcatcagc aacgccgttg taaaagtaat
      961 catcaaaatg aatggcatca acattatact tacgcacaat ttcttcaatg gtatcgtaga
     1021 tatgcttacg caccaaaggt tccgcagggt ttagtaaaat ccgtgcatcg ccgggggcgt
     1081 gaataccccg aaccaataaa tcgggatgtt ttcttgcgaa attaagcggt gacaaagttt
     1141 gcaaataact gtttaattca ctttgataat caccggtatc cggaagtaca ccgagcgatg
     1201 ctcgataagg attcagccag gcatgaaatt ccatattgcg ttcatgacat ctctcaatca
     1261 tccaggccat gggatcccaa cctgggtact gtccctccac attaattaag aagcgactcc
     1321 aaggattgat ggttgaactg taaaaagcat cgttggtcgg ccgcacttga aaaataacgg
     1381 cgttcaaacg ataagcttca aagacatcca aaatcaatca tgttccctaa attagcctat
     1441 ctaaaacaac gaacaacgag taaagtt
//
LOCUS       DAOSGN010000266         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_91035_length_1467_cov_1.260623, whole genome shotgun sequence.
ACCESSION   DAOSGN010000266 DAOSGN010000000
VERSION     DAOSGN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91035_length_1467_cov_1.260623"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..306)
                     /locus_tag="PK087_03975"
     CDS             complement(118..306)
                     /locus_tag="PK087_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH18462.1"
                     /translation="MKITLKAARANKDLTLAEAAKLIGIDTKTLWNYEKSNTYPEVPV
                     IKKIEEVYGLDFKDINFG"
     gene            complement(671..886)
                     /locus_tag="PK087_03980"
     CDS             complement(671..886)
                     /locus_tag="PK087_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18463.1"
                     /translation="MDPAIITALIVACGSVITQVLIAAAGKRATEKIINYRLDALTEK
                     VDKHNHLMERMALAENDIKNIKEVICK"
     gene            complement(886..1092)
                     /locus_tag="PK087_03985"
     CDS             complement(886..1092)
                     /locus_tag="PK087_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18464.1"
                     /translation="MEILIKNLANLLKVKSLITLAMTFGMVMLLSGKWIPPAEIIALF
                     SASYGSVITYFFKKDDKTKIEEGE"
BASE COUNT          358 a          368 c          285 g          456 t
ORIGIN      
        1 atactctcgt aacatcaaaa ggtgttattt ggacatatag attcttgtga tattcttggc
       61 cgactcccta gcctgcttgt cccgcacaag ggtttgcttg tccgacgctt gtccccctta
      121 tccaaaatta atgtctttga agtctagccc gtatacttct tcgatttttt taatcactgg
      181 cacttcggga taagtatttg atttttcgta gttccataaa gttttagtgt ctatccctat
      241 aagcttcgct gcttccgcaa gtgttaagtc tttatttgct cttgctgctt ttaaagttat
      301 tttcatgctt gtccacctcc tttcttccat atcttgccta tataataacc cattggattg
      361 gtaatgtcaa ccattatttt gggaaatatt tcatttctat ataaaaaaag attaagccga
      421 agcccaacct ctccctttcg acgcaacgct taagtggaaa atctgtggaa aataattgtg
      481 gtggcgttaa aaccactcgg caaaactgta taaaaacacc ccccctctaa aatcgccgat
      541 ttaagggggg tttattttgc gacaggatac atagcaccta aacaatactt gccttttcgt
      601 ccgctttggt tccggggcga aacgagcgga atagaaccga acccaataaa aaaagccggg
      661 tttccccggc tcatttgcat attacttctt ttatgttttt aatgtcgttt tcggccaacg
      721 ccatccgctc catcaggtga ttgtgcttgt ccaccttctc cgtgagcgca tccagccggt
      781 aattgatgat tttctccgtc gctcgttttc cggcagccgc tataaggacc tgagtgatta
      841 cacttccaca tgctacgatc agggcggtga tgattgctgg gtccattatt cgccctcctc
      901 aatttttgtc ttgtcatcct tcttgaaaaa gtatgtaatc accgacccgt aggaggccga
      961 aaagagcgct atgatctcgg ccggcgggat ccatttaccc gatagcagca tgaccatgcc
     1021 gaatgtcata gcaagggtta tcagtgattt aacctttaat aaattcgcta aatttttaat
     1081 caatatttcc atcctttcac ctccttgata ccgcaatagt taaccgtcca atatggcgcg
     1141 gccagatgcg ggaccactct cctttcatcg tttgtctcgg atttgaaata ccctgttccc
     1201 gaacttgtcc tcatgatctg tgaaatgcaa accccaacta tataggttca ctccgccgac
     1261 cttcccatga atcgagcaga ttacctgcca tgcgattata aaatcgtcga acctgtcttt
     1321 agggatattg gctatatcgc acgccctccc gtcaaggtgg gcggaaatcg gagaggaacc
     1381 tttcagggtg gcgttaaatg atttagtccg gtaccaactc gacacagtca ttggcttccc
     1441 gtaccagtcc cgtaattcct gcatcat
//
LOCUS       DAOSGN010000267         1458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_91817_length_1458_cov_2.620813, whole genome shotgun sequence.
ACCESSION   DAOSGN010000267 DAOSGN010000000
VERSION     DAOSGN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91817_length_1458_cov_2.620813"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PK087_03990"
     CDS             complement(<1..807)
                     /locus_tag="PK087_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOH18465.1"
                     /translation="MKYLFVINPAAGKKDRSRSIIEQIRALGNLENTEIYLTKGINDG
                     RDFVHQYCLNHGDAPLRIFSCGGDGTLNEVVNGAFGFNNVEIACYPSGSGNDFVKYFG
                     EITDFLDFTQQINGKSMAVDLIRFNNRYAINIFNLGFDADVVVRMQKYKRWPLMSGKS
                     SYIMGVLVSFLNKMGYQFTLTVDDLFLYEGKALLCAVANSICYGGGFYCAPHAKIDDG
                     IIDVILVKKISRFAFIRMISTYKAGRHLETPKVAENIVYGQGKKVVIKCSQ"
     gene            complement(811..>1458)
                     /locus_tag="PK087_03995"
     CDS             complement(811..>1458)
                     /locus_tag="PK087_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18466.1"
                     /translation="GVALKNTAVKHLRFLKKRLDYLNKYGHILYFTETYFVAPQIVCV
                     FEDGLHFDFYVTKMDNINYYDNIAIIYDPHHLLDDYQKKPLSLNSQETANIVHSFCFA
                     AVEFNAAYQRQDLLFAFRLANHMFADLACYLRTVIEPDFARIGLKGFYQNLSEPYRSK
                     YLEIAKYLSLENCLLSVKMMLGYFDQLIINVPIRVAEFLNFEFFQFAKNLLMGME"
BASE COUNT          513 a          280 c          237 g          428 t
ORIGIN      
        1 ttgtgaacac ttaataacta ctttcttgcc ttggccatag acgatgtttt ccgcaacctt
       61 cggggtttca aggtgtcgtc ctgctttata agtactaatc atcctaataa aggcgaatcg
      121 ggaaatcttc ttaactaaaa tgacatcaat aatgccatca tcaattttcg catgaggcgc
      181 acaatagaaa ccaccaccat aacatatgga attagcgacc gcacaaagta aagcttttcc
      241 ttcatacaaa aagagatcat caaccgtcaa agtaaattga taacccattt tatttaaaaa
      301 ggataccaat acgcccataa tataggagct tttccccgac attaggggcc agcgtttata
      361 tttttgcatt ctcaccacaa catcggcatc aaagccgaga ttaaagatgt taatcgcata
      421 acgattatta aaacggatta aatcgacagc cattgatttg ccatttattt gttgggtaaa
      481 atcgagaaaa tcggtaattt caccaaagta ttttacaaaa tcattcccgg aacccgatgg
      541 atagcaagca atttccacat tattgaagcc aaaagcaccg ttgacaactt catttaaagt
      601 gccatcccca ccacagctga atatccgaag aggagcatca ccatggttaa ggcaatattg
      661 atgaacaaaa tcgcgaccat cattaattcc ttttgttaag taaatttcgg tattttctaa
      721 attacctaaa gcacggattt gttcaatgat tgaccgactg cgatcttttt tcccagcagc
      781 gggattaatg acaaataagt atttcatatt ttattccatc cccataagca aatttttagc
      841 aaactgaaag aattcgaaat ttaaaaattc agcaacccta attggtacat tgataattaa
      901 ttggtcaaaa taaccaagca tcattttgac tgataaaaga cagttttcca aactaagata
      961 ttttgcgatt tcaagatact tacttcgata aggttcggac aaattttggt aaaacccctt
     1021 taacccaatt ctagcaaaat caggttcaat aacggttctt aaataacaag ctaaatcagc
     1081 aaacatgtga ttggccaaac gaaaggcaaa taataaatct tgacgttgat aggcggcatt
     1141 aaattcaacg gcagcaaagc aaaaagagtg gacaatattg gccgtttctt gactgtttaa
     1201 acttaaaggc tttttttgat aatcatcaag gagatgatgg gggtcataga taatggcaat
     1261 attgtcataa taattgatgt tgtccatttt agttacataa aaatcaaaat gcaaaccatc
     1321 ttcaaaaacg caaacaattt gcggggccac aaaataagtt tccgtaaaat ataaaatatg
     1381 accgtatttg tttaaataat ctaatcgttt tttaagaaat ctgagatgct tgacggcggt
     1441 attttttaac gctacgcc
//
LOCUS       DAOSGN010000268         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_92403_length_1452_cov_3.396564, whole genome shotgun sequence.
ACCESSION   DAOSGN010000268 DAOSGN010000000
VERSION     DAOSGN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92403_length_1452_cov_3.396564"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK087_04000"
     CDS             <1..233
                     /locus_tag="PK087_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18467.1"
                     /translation="YLDDLEETLADKDLLTDERKEEIREMYGIIEEKLINKTSFDISL
                     FDDFDFRLQGIIPDGLMTTMEIFSMIVEELQL"
     gene            complement(259..>1452)
                     /gene="lysS"
                     /locus_tag="PK087_04005"
     CDS             complement(259..>1452)
                     /gene="lysS"
                     /locus_tag="PK087_04005"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOH18468.1"
                     /translation="EEQFETFKKGDIGDIVGVEGFIIRTDTGELTVKASNYTHLSKAL
                     RPLPEKFHGLQDKEEARRHRYVDLIMNEEAKRIAFLRPKIIRAFQKFFDQKGFVEVET
                     PILQPILGGANARPFITHHNALDMDFYLRIATELPLKRLIVGGMEAVYEIGRLFRNEG
                     MDATHNPEFTTVEAYLAYSDMAGMMDLVEECFRFLTKEVLNKQVITYQGHELRFDVPF
                     RRVHMVEAIKEQTGIDFFKLTSLDEAIDLANKHEIELTKHERAFGHIVNLFFEKYVED
                     TLIQPTFVFGHPLEISPLAKKSVDPRFTERFELFINKKEFANAFSELNDPLDQRERFE
                     NQIKEKALGNEEAAEMDDDFVKALEYGMAPTGGLGIGIDRTVMLLTGADSIRDVLLFP
                     HMRPK"
BASE COUNT          406 a          290 c          312 g          444 t
ORIGIN      
        1 tttaccttga tgatttggaa gaaaccttag cggataaaga cttattaact gatgagcgta
       61 aagaagagat tcgggaaatg tacggaatta tcgaagaaaa actaattaac aaaacttcgt
      121 tcgacatttc gctatttgat gattttgatt ttcgcttaca agggattatt cccgacggtt
      181 taatgacgac catggaaatc ttcagtatga ttgtggaaga actgcaacta tagaaaaaac
      241 caccgccgtg gtggtttttt attttggtcg catatgtggg aataacaaga catcacggat
      301 ggaatcggcg ccggttaata acataaccgt gcggtcaatg ccaatgccta atccgccggt
      361 tggagccatt ccgtattcca aggcttttac aaaatcatca tccatttctg cagcttcctc
      421 gtttccgagg gctttttctt tgatttgatt ttcaaatctt tctctttggt caagcggatc
      481 atttaattcc gagaaagcgt tggcgaattc ttttttattg ataaataatt caaagcgttc
      541 ggtaaagcgc ggatcaacac tctttttggc taaagggctg atttccaagg gatgcccgaa
      601 aacaaaggtt ggttgaatga gggtatcttc cacatacttt tcaaagaata aattgacaat
      661 atggccaaag gcccgttcgt gtttagttag ttcaatttca tgcttattag ctaagtcaat
      721 tgcttcgtcc aaggaagtta gcttaaagaa gtcaatgccg gtttgttctt tgatggcttc
      781 aaccatatgc actcgccgga agggaacatc aaagcggagt tcatgacctt gataagtaat
      841 tacttgtttg tttaaaactt ctttagttag aaagcggaaa cattcttcaa ctaagtccat
      901 catcccggcc atgtccgaat aagctaaata agcttcaacg gtagtaaatt ccggattatg
      961 agtggcatcc atgccttcgt ttcggaatag tctgccgatt tcataaacgg cttccatacc
     1021 gccgacaatc aagcgtttta aaggaagttc ggtggcaatc cgcaagtaga aatccatatc
     1081 taaagcattg tgatgcgtta tgaacggccg cgcattggca ccgccgagga tgggttgtaa
     1141 aatcggggtt tccacttcca caaatccttt ttgatcaaag aacttttgaa aagcgcggat
     1201 gatttttggc cttaaaaaag cgattctctt agcttcttca ttcatgatta aatcaacata
     1261 acggtggcgc cgggcttctt ccttatcttg aagaccgtgg aatttttccg gaagcggtcg
     1321 caaagctttc gatagatggg tgtagttgct ggctttgacg gttaattcgc cggtatccgt
     1381 ccggatgatg aaaccttcaa caccgacgat atcgccgata tcaccttttt tgaaagtttc
     1441 aaattgttct tc
//
LOCUS       DAOSGN010000269         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_92412_length_1452_cov_3.062992, whole genome shotgun sequence.
ACCESSION   DAOSGN010000269 DAOSGN010000000
VERSION     DAOSGN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92412_length_1452_cov_3.062992"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1452
                     /locus_tag="PK087_04010"
     CDS             <1..>1452
                     /locus_tag="PK087_04010"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOH18469.1"
                     /translation="PFFGCATTTDDGYAMIPDGSGVIIDYNNSRSFAMPYQQRVYGKE
                     LAVNARVKENAAEKISFPLFGMKRNKQGFIAIGEDGVEMASILARTSTEDSPYNQAYY
                     RYQFRESEVFVFSAIQSSTNITKWTDWYSLANFKVHYQFLHEDNASYAAMAKKYRQYL
                     LDNQSLQSIDTTKEVMVDLTLLGGYVDKDNFIGIPYSQVRSLTTANEVQIIIEELAQL
                     GINDVRVIYSGWGNDGIKPSYMGKINYNRLVGKKADLEALAKRYPKIIYWEANVAKVF
                     TAQNFNEKDLAVRDVFGKVVVNYAYNEATLYADTSTMSEYLLKPTTYEKTLDNLQKAF
                     QKRGFSNLGLTDFGAYIYGSYEKKETILRPDSLALIKAAGEKLASFEQVIMRNPNVYG
                     LSYLTAITDFPTYGTNYQIVERSVPFAQLVLSGLVDYSAKSFNLDDRYDFSWHKMKAI
                     ETASNISLTWSYEPTIKLTNTEYSHYYSTYYQNW"
BASE COUNT          433 a          267 c          311 g          441 t
ORIGIN      
        1 ccttttttcg gttgtgctac gaccactgat gatggttatg cgatgattcc cgatgggagc
       61 ggggtcatta ttgactacaa taacagccgc tccttcgcca tgccctatca gcaacgcgtc
      121 tatggcaaag aattagctgt taacgcccgt gttaaagaga atgcggctga aaaaatcagt
      181 tttccgctgt ttggcatgaa gcgtaacaag caaggcttta ttgccattgg tgaagacggt
      241 gtggaaatgg cttccatttt agcgcgcacg agtacggaag atagtcctta taatcaggcc
      301 tattaccgtt atcaatttcg tgaaagcgaa gtctttgtct tttctgccat ccaaagttcc
      361 actaatatta cgaagtggac ggattggtat tccctagcca attttaaagt ccactatcaa
      421 tttcttcatg aagataacgc cagttatgcg gcgatggcaa aaaaataccg tcaatattta
      481 ctagataatc aaagtttaca atcaattgat accaccaaag aagtgatggt cgatttaacg
      541 cttttgggcg gatatgttga taaagataat tttatcggta ttccttattc ccaagttaga
      601 agcttaacca cggccaacga agtacaaatc atcattgaag aattagcaca attgggaatc
      661 aatgatgtcc gcgttattta ttccggttgg ggtaatgacg gcattaaacc gagttatatg
      721 ggaaagatta actataaccg tttagttggg aaaaaagctg atttagaagc tttggctaaa
      781 cgatacccca agattattta ttgggaagcg aatgtggcga aagtttttac ggctcagaac
      841 tttaatgaaa aagacttggc ggtgcgtgat gttttcggta aagttgtcgt taattatgct
      901 tataatgaag cgacgctcta tgccgatact tccaccatga gtgaatactt attaaaaccg
      961 actacttatg aaaaaacttt agataattta caaaaggctt ttcaaaagcg cggtttttcc
     1021 aaccttggat taacggattt tggggcctat atctatggtt catatgaaaa gaaggagacg
     1081 attttgcggc cggattcctt ggctttaatt aaagcggccg gagagaagtt agcgtctttt
     1141 gagcaagtga ttatgcgtaa tccgaatgtt tacggcttaa gttatctaac agccattacc
     1201 gattttccaa cttatggtac taattatcag attgtggaaa ggagtgttcc gtttgcgcaa
     1261 ctggttttaa gcggcttggt ggattacagt gcgaaatcct ttaatctcga tgatcgttat
     1321 gacttttctt ggcataaaat gaaagctatc gaaacagctt ccaatatcag tttgacttgg
     1381 agctatgaac cgaccattaa attaaccaat acggaatatt cccattatta ttccacatat
     1441 taccaaaatt gg
//
LOCUS       DAOSGN010000270         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_92598_length_1450_cov_2.792832, whole genome shotgun sequence.
ACCESSION   DAOSGN010000270 DAOSGN010000000
VERSION     DAOSGN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92598_length_1450_cov_2.792832"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK087_04015"
     CDS             <1..430
                     /locus_tag="PK087_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18470.1"
                     /translation="NVFSLIATLRDSSVPLVYVQGLNLEHEVIAKAAAQNYLDFYQQE
                     ITTRQNLFYPPFCEINRLVIIGPYRNIYHYANYFKKVFRRISRGEALGPVYLPAVRGV
                     QLILKHEDFPKVSQLIDEVNEKFKPQKLIVNFERYPLSFR"
     gene            439..1365
                     /gene="fmt"
                     /locus_tag="PK087_04020"
     CDS             439..1365
                     /gene="fmt"
                     /locus_tag="PK087_04020"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOH18471.1"
                     /translation="MKIIFMGTPLFAVNILESLATQHEILMVVTQPDQGTKNKPLIPA
                     VKTWALNHHFELRQPPKIATLTEEILSLPVDLIVTAAYGQFIPLKILHHPRFQSINVH
                     GSLLPKYRGGAPIQRAIMNGDKETGISIIRMVKRMDAGPILAQQALPILDSDNQDSLF
                     SKLSELGAKMILRTIEELQNNPLLGQAQDETKATYAFNLQKEDEKLDFSQTALTCFNR
                     IRGLCSNPGAFFQLDGLNIKVYNSVVSPLIPNQAPGVIVEKTKKHFTISCGGNTALSM
                     LEVQLPSGKRMATQDFLNGKGKDLIVLHKEIK"
BASE COUNT          441 a          284 c          277 g          448 t
ORIGIN      
        1 aaatgtcttt tcattaattg ccactcttcg tgattcttcg gtacccttag tttatgttca
       61 aggcttaaac cttgagcacg aagtgattgc gaaagctgct gcccaaaatt atcttgattt
      121 ctatcaacaa gaaattacca ctcgacaaaa tttattctat ccgccttttt gtgagattaa
      181 tcgcttggta attatcggtc cttatcgcaa catctatcat tatgctaatt attttaaaaa
      241 agtatttcga cgcatcagtc gcggtgaagc tttggggccg gtttatcttc cggcagttag
      301 aggagttcaa ctcatactca agcatgaaga ttttcccaag gtttcgcagc tcattgatga
      361 agtgaatgaa aaatttaaac cgcaaaagct gattgttaat tttgaaagat atcctctttc
      421 atttcgttag gagcctttat gaaaattatt tttatgggaa cacccttatt tgctgttaat
      481 attttagaaa gcttggctac ccaacatgaa atcttgatgg tcgttactca acctgaccaa
      541 ggaacaaaga ataaaccctt gattccggcg gttaaaacct gggctttaaa tcatcatttt
      601 gaacttagac agcctccgaa aatagcgaca ttaaccgagg aaattttatc tctccccgtt
      661 gatttaatcg taacggctgc ttatggtcaa tttattcctt taaaaattct tcatcatccc
      721 cgttttcaaa gcattaatgt tcatggctcc ttgttaccca aatatcgggg tggggcccct
      781 attcaaagag ccattatgaa tggggacaag gaaacgggca tttccattat tcgtatggtt
      841 aaaagaatgg atgcgggacc aattttggcc caacaagccc tccccatttt ggattctgac
      901 aaccaagatt ccctgttttc taaattaagc gagttaggag caaagatgat tttgcggacg
      961 attgaggaat tacaaaacaa tcctttatta gggcaagctc aagatgaaac aaaggctact
     1021 tatgctttca atcttcaaaa ggaagacgaa aaactggatt tttcccaaac agccttgacc
     1081 tgctttaatc gcattcgggg cctatgttcc aatccgggcg ctttttttca acttgatgga
     1141 ttaaatataa aggtttataa tagtgttgtc agccccttaa tcccgaatca agcccccggt
     1201 gtaattgttg aaaaaaccaa aaaacacttt acgataagtt gtggtggcaa caccgcttta
     1261 tcgatgcttg aagttcagct accttcggga aaacggatgg caactcagga ctttctaaat
     1321 ggcaaaggga aagatttaat tgtattgcat aaggagatta aataatgaaa ccaagaattt
     1381 atttttcacg tgaagaaatg tttaaactaa accgtgagcg cttattggag cgtggtgtta
     1441 aaatcgaaga
//
LOCUS       DAOSGN010000271         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_92790_length_1448_cov_3.225413, whole genome shotgun sequence.
ACCESSION   DAOSGN010000271 DAOSGN010000000
VERSION     DAOSGN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92790_length_1448_cov_3.225413"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..341
                     /gene="rpsS"
                     /locus_tag="PK087_04025"
     CDS             63..341
                     /gene="rpsS"
                     /locus_tag="PK087_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010307968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOH18472.1"
                     /translation="MARSLKKGPFCDDHLMKKVLAQTATNKKVIQTWSRRSTIFPEFI
                     GLTIGVYNGKSHIPVYVTEDMVGHKLGEFAPTRTFRGHGKDKKATDKK"
     gene            353..688
                     /gene="rplV"
                     /locus_tag="PK087_04030"
     CDS             353..688
                     /gene="rplV"
                     /locus_tag="PK087_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOH18473.1"
                     /translation="MEAKAIAKVVRIAPRKVRLVIDLVRGKKLAEANAILRLTPKAAS
                     RVVEKVIKSAAANAVNNYKLDQGKLFVKEAYVNEGPTMKRFLPRAKGSASAILKRTSH
                     ITVVLAEKE"
     gene            700..1356
                     /gene="rpsC"
                     /locus_tag="PK087_04035"
     CDS             700..1356
                     /gene="rpsC"
                     /locus_tag="PK087_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOH18474.1"
                     /translation="MGQKVNPIGLRVGIIRDWDSKWFANKLEVPALLHEDLKIRKYLS
                     NFYKKAYVSKVEIERTKTKIMLTIYTAKPGVVIGREGATKTQAVEELQKLTGKTVFLS
                     VVEIRNPDLDAVLVARNIAEQLENRASFRRVQKMAIQKTMRAGAKGIKTKISGRLGGA
                     EMAREEGYSEGTVPLHTLRADINYACDEAKTTYGKLGVKVWICLGEVLPEKKNKQGDK
                     "
     gene            1359..>1448
                     /locus_tag="PK087_04040"
     CDS             1359..>1448
                     /locus_tag="PK087_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOH18475.1"
                     /translation="MLMPKRTKFRRPHRVSYEGKAKGGKEINFG"
BASE COUNT          495 a          262 c          334 g          357 t
ORIGIN      
        1 caagaaaaag tcctctaatt taattattag acgacgagta agcaattagg aggaaaaagt
       61 ttatggcacg ttcattaaaa aaaggcccgt tttgcgatga tcacttaatg aaaaaagttc
      121 ttgcgcaaac cgccaccaac aaaaaagtga ttcaaacatg gtcgcgtcgt tctaccattt
      181 tccccgaatt catcggctta accattggtg tttacaatgg gaaatctcat attccggttt
      241 atgtaactga agatatggtc ggtcataaac taggagaatt cgctcctaca agaactttcc
      301 gtggccatgg aaaagataaa aaagccacgg ataagaagta aggaggaaga atatggaagc
      361 caaagcaatt gcgaaagttg ttcgtatcgc tcctcgtaaa gttcgcctcg ttatcgattt
      421 agtccgtggc aaaaagcttg cggaagctaa tgcgatttta cgactgaccc cgaaagccgc
      481 cagcagggtt gtggaaaaag taatcaaatc ggcagctgct aatgcggtta ataattataa
      541 attagatcaa ggcaagttgt tcgttaaaga agcatatgtt aacgaaggac caacgatgaa
      601 aagattttta ccaagagcaa agggaagtgc ctcagccatt ctgaaaagaa cgagtcatat
      661 taccgttgtt ctggctgaga aagaataagg aggaatctca tgggacaaaa agttaatcca
      721 attggcttaa gagtcggcat cattcgtgat tgggattcta aatggttcgc taataaatta
      781 gaagtaccgg ccttattaca tgaagattta aaaattcgta aatacttaag caatttttat
      841 aagaaagctt atgtatcaaa agtagaaatt gaacggacca aaaccaaaat catgttaacg
      901 atttataccg ctaaacccgg tgtggtcatt ggtcgtgaag gtgcaaccaa aacccaagcg
      961 gtggaagaat tgcaaaaatt aaccggcaaa acggttttct taagtgttgt agaaatcagg
     1021 aatccggatt tggatgcggt tttagttgct cgcaatattg cggaacaact ggaaaaccga
     1081 gcttccttcc gtcgtgtcca aaaaatggcc attcaaaaaa ccatgcgggc cggagctaaa
     1141 gggattaaaa ccaaaatttc cggtcgctta gggggagcag aaatggctcg tgaagaaggc
     1201 tacagtgaag gcaccgtccc gcttcatacc ttgcgggcgg atatcaatta tgcctgtgac
     1261 gaagccaaaa ccacttatgg gaaattaggc gttaaagttt ggatttgctt gggggaagtt
     1321 ttaccggaaa agaaaaacaa gcagggggat aaataattat gttgatgccc aaaagaacaa
     1381 aattccgccg ccctcatcgc gttagttatg aaggtaaagc gaaaggcgga aaagaaatta
     1441 atttcggt
//
LOCUS       DAOSGN010000272         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_92847_length_1448_cov_2.185930, whole genome shotgun sequence.
ACCESSION   DAOSGN010000272 DAOSGN010000000
VERSION     DAOSGN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92847_length_1448_cov_2.185930"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PK087_04045"
     CDS             <1..632
                     /locus_tag="PK087_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18476.1"
                     /translation="RFAYEDHLILEDINLEFEKGKKYLIVGPSGGGKSTILKLLRKYF
                     YPEQGSILIDGIPLKDIKKEQYFSHIANVEQNVFLFEDTIRHNLTLYKEYTDEEINEA
                     IAKAGLKDFIAKLPQGLETMIYDNGKNISGGERSRLAIARGLINQADIIFLDEAFANL
                     DAEKAKAIEKSLLDLKDVTIINVSHIVFKEHRPMYDDVIVVKNKMATSL"
     gene            complement(738..>1448)
                     /locus_tag="PK087_04050"
     CDS             complement(738..>1448)
                     /locus_tag="PK087_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOH18477.1"
                     /translation="NNPALKQSRILTNFGGDIGDIITLAHELGHAYHGEQIFKEALLN
                     SHYTMPVAETASTLCETITKRAAYREATSKDEKIHILEQELQDDTQVIVDIYSRFLFE
                     QAVFSAREQSIPSAKELNAMMKDAQKAAYGKALDANTLNGGMWINKSHYYRGGLSFYN
                     FPYAFGLLFAKGIYAKYMEVGSSFVEKINQLLRATGKMNCEDVAKLIDIDITKTEFWE
                     AGLKMIAEDVDEFIALTK"
BASE COUNT          451 a          283 c          270 g          444 t
ORIGIN      
        1 ttcgttttgc ttatgaagat catttaatct tggaagatat taacttggaa tttgaaaaag
       61 gcaagaaata tttaattgtt gggccttccg ggggcggaaa gtcaacgatt ttaaaattgt
      121 tgcgcaaata tttctacccc gaacaaggaa gtattttaat tgatggtatc cctttaaaag
      181 acatcaaaaa agaacaatat ttttcccata ttgctaatgt tgaacaaaat gtctttttgt
      241 ttgaagatac gattcgccat aacttaacat tgtataagga atatactgat gaagaaatca
      301 acgaagcgat tgcgaaagcg ggactaaagg attttattgc caagctgccc caaggtttag
      361 aaacaatgat ttatgataac ggcaaaaaca tcagtggcgg agaacgaagc cgtttagcga
      421 ttgctcgcgg cttaatcaat caagctgaca ttattttcct ggatgaagcc tttgctaatt
      481 tagatgcgga aaaggccaaa gccattgaaa aaagccttct cgatttaaaa gatgtgacca
      541 tcattaatgt cagtcacatt gtctttaaag aacatcgccc catgtatgat gatgtcattg
      601 ttgtgaagaa caaaatggct accagtttat agccaaagag acggtaaaga aataatctaa
      661 taaaagtcac aaccatataa aaagccaagt aaatgtcaga attcatctga tccacttggc
      721 ttttttactg tatctgatta tttggttaaa gcaataaact catcaacatc ttccgcaatc
      781 atctttaagc ccgcttccca aaattccgtt ttggtaatgt cgatgtcaat tagtttcgca
      841 acatcttcac aattcatttt tccggttgct cttaacaatt ggttgatttt ttcgacaaac
      901 gagcttccca cttccatgta tttcgcataa atccccttcg caaacaacaa gccaaaagca
      961 taagggaaat tataaaaact taagccgcca cgataataat gcgatttgtt gatccacatg
     1021 ccgccattta aggtattggc atctaaagct tttccataag ccgctttttg ggcatctttc
     1081 atcatggcat taagttcttt agcactcgga atcgattgtt cccgggcact gaaaaccgct
     1141 tgttcaaaca agaaacgact ataaatatca actatgactt gggtgtcgtc ctgtagttct
     1201 tgttcgagaa tatggatttt ctcatccttg cttgtggcct cccgataagc tgcccgtttg
     1261 gtaatcgttt cacataatgt ggaagccgtt tccgcaaccg gcatcgtata atggctgttt
     1321 aacagtgctt ctttaaagat ttgttcacca tgataagcat gaccaagttc atgggctaaa
     1381 gtaatgatat caccgatatc cccaccaaaa ttcgtgagaa tccggctttg ttttaaggcg
     1441 gggttgtt
//
LOCUS       DAOSGN010000273         1446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_93018_length_1446_cov_2.980590, whole genome shotgun sequence.
ACCESSION   DAOSGN010000273 DAOSGN010000000
VERSION     DAOSGN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93018_length_1446_cov_2.980590"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PK087_04055"
     CDS             <1..1371
                     /locus_tag="PK087_04055"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOH18478.1"
                     /translation="YIHMSIVDIYDIETLVKVDSYQITGYIEGSRLIKDNLYLISNYQ
                     PNYYDLKNEETDIRPWYIHNGEAKYFDYSEIKYLPDSFYQAFNIITTINLGKERSIAN
                     DVFLSASYWGQIYVSPTAIYFAANYYTRSFINDLFGTWKQKGLLFSFQINSKTGEVSF
                     GGAGEFEGIVINQFAMDEYNGYMRLATTDGWGEDVVNRLYVFERKLVDEAYKLEVVGK
                     IDSGLGKPGESIQSVRFNKDIATIVTFLRTDPFYTVDLKDPYNPKIIGALEIPGFSTY
                     QHPWTDTLILGIGFDAVDGRTTGMKLALYDITDQENPVEVGKPLILSNGQNSWSYSEA
                     TWNHKAIMIDKTRNCFGFTLWRSNWDNYYYSSTNDYVIFSVDETKETPITLKYSFSHI
                     NYFQQNKSMYEGYYWRYDFAIKRAFRVDDYLYVISGEVATSHNLLGDLSVVDDIIFEK
                     AFVDAK"
BASE COUNT          456 a          270 c          259 g          461 t
ORIGIN      
        1 tacattcaca tgagtattgt tgatatttat gatattgaaa cgcttgtgaa agtcgacagt
       61 tatcaaatta ccggctatat tgaaggttct cgcttaatta aagataattt atatttaatt
      121 tccaattatc agcccaatta ttatgattta aaaaatgaag aaaccgatat ccgaccatgg
      181 tatattcata acggtgaagc caaatacttt gattattcgg aaatcaaata tttgcctgat
      241 tcgttttatc aagcctttaa cattattaca accattaatt taggtaaaga aagaagcatt
      301 gctaatgatg ttttcttatc agcatcttat tggggacaaa tttatgtgtc accaacggcc
      361 atttatttcg ccgccaatta ttatacaaga agttttatca acgatttatt tggcacttgg
      421 aaacaaaagg gcttattatt ttctttccaa atcaacagca aaacgggcga agtttccttc
      481 ggcggtgccg gtgaatttga aggcatagtc attaaccaat ttgctatgga tgaatacaat
      541 ggctatatgc gtttagctac caccgatggt tggggcgaag atgtcgttaa ccgtctatat
      601 gtttttgaac gaaagttagt tgatgaagcc tataaattag aagttgtcgg taaaattgac
      661 tccggacttg gaaaaccggg cgaaagcatc caatccgttc gtttcaataa agatatcgct
      721 accatcgtta ccttcttaag aaccgatccc ttctacaccg ttgatttaaa ggatccctat
      781 aatccgaaaa ttattggtgc tttagaaatc cccggtttca gcacctacca acatccttgg
      841 accgatactt taattttagg aattggcttt gatgctgttg atggcaggac cactggcatg
      901 aagttagcac tttatgatat cactgaccaa gaaaacccgg ttgaagtcgg taaaccctta
      961 attttaagca acggtcaaaa ttcttggtcc tacagtgaag ccacctggaa ccataaagcg
     1021 attatgattg ataaaacccg taactgcttc ggctttacat tatggcgttc aaactgggat
     1081 aattattatt actcaagcac gaatgactat gtgattttct ccgttgatga aactaaagaa
     1141 acaccgatta cacttaagta ttctttcagc catatcaatt atttccaaca aaataagagc
     1201 atgtatgaag gctactactg gcgttatgat ttcgctatca aacgcgcttt ccgtgtcgac
     1261 gattatctct atgtcattag tggtgaagtc gcaacttcgc ataatttgct tggtgattta
     1321 tccgttgttg atgatatcat tttcgaaaaa gccttcgttg atgctaaata agaataaacc
     1381 aaattatcgt taatcccacc tcataattga ttaggaatga gcaaaatgat ataattaaca
     1441 taaccc
//
LOCUS       DAOSGN010000274         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_93249_length_1444_cov_2.325414, whole genome shotgun sequence.
ACCESSION   DAOSGN010000274 DAOSGN010000000
VERSION     DAOSGN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93249_length_1444_cov_2.325414"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PK087_04060"
     CDS             complement(<1..430)
                     /locus_tag="PK087_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18479.1"
                     /translation="MLSLDNWYKLNKIYATNSAFIKDNSYTKLGTFNSDFKRDFKRIR
                     EVMLVLTKENYEKRKTDAGLNRIRARKKFYQNIKLYEENNIKPTSFGDFHKSFKEWLY
                     LEEIAVKKSNKPQNMAYINELKGFLNTIDIIKGKKADINQD"
     gene            396..>1444
                     /locus_tag="PK087_04065"
     CDS             396..>1444
                     /locus_tag="PK087_04065"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="catalyzes the formation of alpha-D-glucose
                     1-phosphate and UDP-galactose from UDP-glucose and
                     alpha-D-galactose 1-phosphate in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HOH18480.1"
                     /translation="MFNLYQLSKDSNYIQTKRISQNVHWQEKLEYGELAMTINLSKPE
                     KDPKLIALQKTNKSSSYPKCQLCVENEGYRGHLSYDARSNMRIIPITLNKEPWYFQYS
                     PYSYFNEHAIVLHEEHKPMIVDGSTFRKLLDFLDLFPTYFIGSNAGLPIVGGSILNHE
                     HYQAGKHHFPIEKAQASFVRRVGDVTVERLIWPLSTIRLLSPNKEQIITMATHILEKW
                     QSYQNPALSLLNPSFGFHHTLTPIARLEQKQYVFDLILRSNYVNQEYPDGVFHPHPDC
                     HHIKKENIGLIEAMGMGILPPRLKFELDLVGKVLQGKDELRRDEGLQKHADWLNYLQT
                     IYDGKEDVQTFIYKQT"
BASE COUNT          445 a          270 c          236 g          493 t
ORIGIN      
        1 tatcctgatt tatatcggcc tttttaccct ttataatatc tatcgtgttt aaaaaacctt
       61 ttaattcgtt aatataagcc atattttgcg gtttgtttga ttttttcacc gcaatctctt
      121 ctaaatagag ccattcttta aaagatttat gaaaatcccc aaaagaagta ggttttatat
      181 tgttttcttc atataatttt atattttgat agaatttttt tctcgctcgt atcctgttta
      241 aacctgcatc tgtttttctt ttttcataat tttcttttgt aaggacaagc ataacctctc
      301 tgattctttt aaaatcgcgt ttaaaatcac tgttgaaagt tccaagtttt gtatatgaat
      361 tatcttttat aaacgcactg ttagttgcat atatcttgtt taatttatac caattatcca
      421 aagacagcaa ttatatccag accaaacgga tttcgcaaaa tgttcattgg caagaaaaac
      481 ttgaatatgg tgaactagca atgacgatta atttatcgaa accggaaaaa gatccgaaat
      541 taatcgccct tcaaaaaaca aataaatcat caagctatcc caaatgtcaa ttatgtgtcg
      601 aaaatgaggg ctaccgcggt catctttcct atgatgctcg cagcaatatg cgcattattc
      661 ctattactct gaacaaagaa ccatggtatt ttcaatattc cccttattcc tattttaatg
      721 aacatgccat tgttttacat gaagaacata aaccgatgat tgttgatgga agtacttttc
      781 gaaaattact ggatttttta gatttgtttc cgacttattt catcggttcc aatgccggct
      841 tgcccattgt tggaggttcg attttaaacc atgaacatta ccaagccggt aaacatcatt
      901 ttccgattga aaaagcccaa gctagttttg tgagaagagt tggggatgtg acggttgaaa
      961 ggctaatttg gccgcttagt acgattcgtt tgctttcgcc caataaagag caaattatca
     1021 ccatggccac gcatattttg gaaaaatggc agagttacca aaatcccgct ctttccttgc
     1081 ttaatccctc atttggtttt catcacacct taactccgat tgcccgcctt gaacaaaaac
     1141 aatatgtttt tgatttgatt ttacgttcaa attatgtcaa ccaagaatat cccgatggcg
     1201 tttttcatcc gcaccccgat tgccatcata ttaaaaaaga aaatatcggt ttgattgaag
     1261 ctatgggcat gggcattttg ccaccaaggt tgaagtttga actggattta gttggaaaag
     1321 ttctccaggg aaaagatgaa ttaagaaggg acgaagggct tcagaagcat gccgattggc
     1381 tcaactattt acaaacaatc tatgatggaa aagaagatgt ccaaaccttc atttataaac
     1441 aaac
//
LOCUS       DAOSGN010000275         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_93997_length_1436_cov_3.196959, whole genome shotgun sequence.
ACCESSION   DAOSGN010000275 DAOSGN010000000
VERSION     DAOSGN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93997_length_1436_cov_3.196959"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PK087_04070"
     CDS             complement(<1..465)
                     /locus_tag="PK087_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18481.1"
                     /translation="MKNFKRVFKYMKGRYHLLVFSVILIIIVQLLGFLTPLIVKTILD
                     DYILGIEYPWVEVAREDEKTVTYNHRFFKQERFLDSDDVSLGAASIVINKTSFYLVYD
                     RVESGNRTFENKQIVVQQANGLVFRYDATKLDRMAVFLFYQPMVDKLIFWIVF"
     gene            complement(452..>1436)
                     /locus_tag="PK087_04075"
     CDS             complement(452..>1436)
                     /locus_tag="PK087_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18482.1"
                     /translation="SYFVAIAYGSYMVIKSQISTGELVTFLVYVGMLYGPLVALSNVL
                     NTSNNIIISDERFYEIMDLQPEVKDEVEAQPILSFKTIEFKNVSFKYPFDNFHVIENI
                     SFTINSGETIGIVGPTGAGKSTLIRQLLREFNITGRQILFDGVNINRLKMEDARNLVG
                     YVPQNHVLFRRSVDDNILIGNPKASPAEVASAVSLADFKKDLKELPLGMETMVAELGG
                     SLSGGQRQRLSIARALVKNPEILILDDSLSAVDALTESNIIRNLKQARSGKTNIIIAH
                     RFSAVAEADKIIVLQNGKISAIGTHLELLKYDNWYKMQYLNQIKGDRYEEL"
BASE COUNT          458 a          313 c          251 g          414 t
ORIGIN      
        1 gaacacaatc caaaagatga gtttatcgac catcggttga taaaacaaaa agacggccat
       61 acgatcaagt ttcgtagcat cataacgaaa aactaaacca ttagcttgtt ggacaacaat
      121 ttgcttgttt tcaaaagtgc ggttgccact ttccacacga tcgtaaacaa ggtaaaaact
      181 tgttttatta ataacgatac tggccgctcc taaactaaca tcatcgctat caaggaaacg
      241 ttcctgttta aagaaacgat gattataagt aacagttttt tcgtcctccc ttgcaacttc
      301 cacccaagga tattcaattc ctaaaatata atcatccaag atcgttttca caatcagcgg
      361 cgtcaaaaac cctaataact gaacaataat aatcagaata acggaaaaaa ccagtaggtg
      421 ataacgccct ttcatatact tgaaaacccg tttaaagttc ttcatagcga tcacctttga
      481 tttggttaag atattgcatc ttataccaat tatcatattt taataattct aaatgtgtgc
      541 cgatggcact tattttcccg ttttgtaaga caatgatttt atcggcctcc gcaacagctg
      601 aaaaacgatg ggcgatgatg atattcgttt ttccgctgcg agcctgtttt aaattgcgaa
      661 taatattgct ttcagttaag gcatcaacag cactgagaga atcatcaaga attaggattt
      721 ccgggttttt cacaagcgct ctggcaatcg acaacctttg cctttgacca ccggaaagtg
      781 atccccccaa ttccgcaacc atcgtctcca tccctaaagg caattctttt aagtccttct
      841 tgaagtcggc caaagaaacg gcactggcta cttctgccgg actggctttc ggattgccga
      901 ttaaaatatt atcatcgacg gagcgccgga aaaggacatg gttttgggga acataaccca
      961 caaggttgcg ggcatcttcc atctttagtc gattaatatt aacaccgtca aaaagaattt
     1021 gcctaccggt gatattaaac tcccgaagca attggcggat gagagttgat ttccccgctc
     1081 cggtcggacc gactatcccg attgtttccc cgctgttaat cgtaaaagag atattctcaa
     1141 tgacatggaa attatcaaag ggatatttaa aactgacatt cttaaattct atcgttttaa
     1201 aggagagaat cggttgcgct tctacttcat ccttaacttc cggttgcaaa tccataattt
     1261 cataaaaacg ttcatcggaa ataataatat tattgcttgt gtttaaaaca ttgcttaatg
     1321 cgactaaggg tccatagagc atgccgacat aaaccaagaa cgttactaat tccccggtcg
     1381 aaatttgtga tttaatcacc atataactac cataagcaat cgctacaaaa taggaa
//
LOCUS       DAOSGN010000276         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_94503_length_1431_cov_3.745640, whole genome shotgun sequence.
ACCESSION   DAOSGN010000276 DAOSGN010000000
VERSION     DAOSGN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94503_length_1431_cov_3.745640"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1431
                     /locus_tag="PK087_04080"
     CDS             39..>1431
                     /locus_tag="PK087_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18483.1"
                     /translation="MYFEKDDIYIQQISRPVENSLLISFLMSIKNINISVPDKLDNIF
                     AMDRFCMSFTKHEELELKYENYLTFAFLRENEIKIKHDKFISAKSMPDYLHDQLFDDA
                     NADNYYLFALNRSQRYVVQTAQEGNNFVISGRLGTGKTTALVNVAINAMSQGKRVLYL
                     SNMKTTLDEVFSLLESKNLSQYASNFTNLFSNIHKWEQTISPKAYSAKEEFSALIDNY
                     QFVQDYEQKMGERILDQRFIDIVNALIMLKQKPHQFLEIDDLQHLYKAEFLEIVNALE
                     IIQQSLGKMASFKNNIWRDIPIYNSIKYPNQIVSLIYQIHKCFKILEAEKSVLENDFG
                     FKTIANYAYLKNIIRSINNLNIHEVPEAWKEPSNQSFFQANELYKQLKQEIYQLQERE
                     RFLEHKYDYFDTINIKQDLANIEGHYFNESESEEINQIFANRMDIIVLVNKCQMQEDV
                     FRKTAEKVRTMLSW"
BASE COUNT          506 a          232 c          244 g          449 t
ORIGIN      
        1 aagaaatctt ttctcccata attctcattc cggtgaattt gtattttgag aaagatgata
       61 tttacatcca acaaatttcc cgcccggtgg aaaactccct tttaatcagt tttttaatgt
      121 caatcaaaaa cattaatatt tccgttcccg ataaacttga taatattttt gccatggatc
      181 gtttttgtat gagctttaca aaacacgaag aattagaact gaaatatgag aattatttga
      241 cctttgcttt tttaagagaa aatgaaatta aaattaaaca tgacaaattt atttctgcca
      301 aaagcatgcc tgattattta catgatcaat tgttcgatga tgccaatgcg gataattatt
      361 atttgtttgc ccttaatcgc tcccaaagat atgttgtgca aactgcccaa gaaggaaaca
      421 attttgttat ctcgggtcgt ttaggaacgg gaaaaacgac ggctttagtt aatgtggcca
      481 tcaatgctat gagtcaaggt aagcgggtcc tatacctttc caatatgaaa acgaccttgg
      541 atgaggtgtt ttcgcttttg gaaagcaaaa acctctccca atatgcaagc aattttacca
      601 atttgttttc taacattcat aaatgggaac aaaccatcag tccgaaagct tatagcgcta
      661 aagaagaatt ttctgcctta atcgacaact atcaatttgt tcaggattac gaacaaaaaa
      721 tgggggaaag aattttagat caacgtttta ttgatattgt taacgccttg attatgttaa
      781 agcaaaaacc tcatcagttt ttagagattg atgatttgca acatctatat aaggctgaat
      841 tcttagaaat cgttaacgct ttagaaatca tccaacaatc gcttgggaaa atggcgagtt
      901 ttaaaaacaa tatttggcgg gatattccca tttataacag cattaaatat cctaaccaaa
      961 ttgtgtctct tatctatcaa attcacaaat gttttaaaat tttagaagcg gaaaaaagtg
     1021 ttttagaaaa tgattttggg tttaaaacga ttgccaatta tgcctattta aaaaatatca
     1081 ttcgcagtat taataattta aatattcatg aagttcccga ggcatggaag gaaccaagca
     1141 accaaagctt tttccaagcc aacgagcttt ataaacaatt aaaacaagaa atctatcaac
     1201 tccaagaaag agagagattt ttagagcata aatatgatta tttcgatacc atcaatatca
     1261 agcaagattt agctaatatt gagggccact attttaatga aagcgaaagc gaagagataa
     1321 accaaatttt tgctaatcgg atggatatca tcgttttagt taataagtgc caaatgcaag
     1381 aagatgtctt taggaaaacg gcggaaaaag tccgaacgat gttgagttgg g
//
LOCUS       DAOSGN010000277         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_95139_length_1426_cov_1.691466, whole genome shotgun sequence.
ACCESSION   DAOSGN010000277 DAOSGN010000000
VERSION     DAOSGN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95139_length_1426_cov_1.691466"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1326
                     /locus_tag="PK087_04085"
                     /pseudo
     CDS             119..1326
                     /locus_tag="PK087_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004417810.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
BASE COUNT          409 a          237 c          359 g          421 t
ORIGIN      
        1 atatgaataa atattcatat tgtccttttt cgatttattt attttttgtc tattcagctt
       61 tttctaatag gcatttattt attaatcgat gtgaaagcgt tttattaatg gaggtattat
      121 gagtaacaaa gtttacattg ttggggccaa acgcagtccc aacggctcgt tccttggagc
      181 ttttaaggat gttcatccgg ccgaattagg cgctcaagtg ttaaaaaaat tgttggcgga
      241 aagccgtgtg ccggtggcaa aaatcgatga agtgattgtc ggccaagttt tatctgctgg
      301 tgttggtcaa ggttttgcca gacaagtctc gttaaaagcg ggggtgccga ttgaagttcc
      361 ggcatattct cttaatatgg tttgcggcag tggatgaaat caattatgaa tgcttatgcc
      421 agcattaaag ctaagatggc gaatttaatc gttgccggtg gtgttgaatc gatgagtcaa
      481 gccccttatt tggtgcctgc taaagctcgt gatggcgttc gcatgggcga cttaaattta
      541 gctgatcatc ttttaaaaga tgccttagtt gatgtcttta ataattatca catgggcgtt
      601 acggcagaaa atattgtaaa gaaatatggc atcagccgtg aagaacaaga tgcctttgct
      661 ttaaaatcgc aagaaaaagc cattaaggcg gttgatagtg gtgcttttga tccggaaatt
      721 gtcccgattg aagttaaaac aaggaaagaa acactgctta tcagtcgtga tgaatatcct
      781 aatcggacaa cgaatgcaga aaaaatggct aaactacgtc cggccttttt accggatgga
      841 acggttaccg ctgggagctc ctcagggatt aatgatggcg ctagctttgt gattgttgct
      901 tctgaagaag cggtcaaaca atatcaatta ccggtgcttg cggaaatcat cagtgttggc
      961 caaggcggcg ttgatccatc cgtcatgggt ttgggtccgg ttcccgccat tagagaagct
     1021 ttaaataatg ccaagatgac tttaaaacaa atggaattag tggaattaaa tgaagctttt
     1081 gcggctcaaa gtttaggtgt tgtgaaagaa ttagtggaag aacatcagat tgatcgcgat
     1141 gagcttttaa ataaaaccaa tattaacggt ggcgcgattg cccttgggca tccactgggc
     1201 aatagtggga accgcattac tgttacctta ctgtatggat taatggcgac caaacgtact
     1261 tacggtctag caagcttatg tattggtggc ggtatgggaa cagccatcgt gattaagaga
     1321 gtataagagg agggaaaaaa atgaaattac aaggaaaagt tgctgttgtt actggtggtg
     1381 ccaaaggaat tggtgccgaa attgcgaaag cctttgcttt agaagg
//
LOCUS       DAOSGN010000278         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_95912_length_1418_cov_2.966985, whole genome shotgun sequence.
ACCESSION   DAOSGN010000278 DAOSGN010000000
VERSION     DAOSGN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95912_length_1418_cov_2.966985"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..651)
                     /gene="mscL"
                     /locus_tag="PK087_04090"
     CDS             complement(163..651)
                     /gene="mscL"
                     /locus_tag="PK087_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOH18484.1"
                     /translation="MRKFLKEFGQFIQRGNVLDLAVGVIIGGAFGKIVSSLVKDVLMP
                     LIGLFVGGNLAEMSWELTPAVMNGAEIVKPATTLNYGLFIQTVIDFLIISLVIFTLLK
                     AIMKARKIAEKAKADREAKAAHEKAQVEAVAEPVPAKPTTEELLTEIRDLLAKPKPKP
                     KA"
     gene            complement(799..>1418)
                     /locus_tag="PK087_04095"
     CDS             complement(799..>1418)
                     /locus_tag="PK087_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18485.1"
                     /translation="FFNRYDYRDLAEDELRKQFSIVFQDFQIYAVTIGENVLMREIKT
                     LEDENLVWEALDKVGLRNFVENLPDGLNTLVTREFVQDGVVFSGGDRQKLAIARVFAS
                     DAQIYILDEPTSALDPYSEKAINDLIITNAANKTIIIIAHRLSTVVDVDHIILIEHGQ
                     IVESGTHEQLINLQQRYYHLFSTQASMYKKNNDNQIIESDMEDTI"
BASE COUNT          445 a          286 c          241 g          446 t
ORIGIN      
        1 agaccaagta accttgctta gcaaggtatt cccccgttac taaaccaatt cctgaagaag
       61 caccagtaat aactatcgtt ttcatctttt tcaccttaat taattataaa gaaacaatgc
      121 ttaaaatgca agtaaaaagc caccaagcgg tggcctaatg atttacgcct ttggttttgg
      181 tttaggctta gccaataaat cacgaatttc ggtaagtaat tcttccgttg ttggtttggc
      241 aggaaccggt tccgctacgg cttcgacttg ggccttctca tgtgcagctt tggcttcacg
      301 gtcagcctta gccttttccg caatctttct cgctttcatg atggctttaa gaagcgtgaa
      361 aatgactaaa gaaataataa gaaaatcaat aactgtttga ataaataagc cataatttaa
      421 ggtggtcgcc ggtttcacaa tctcggcacc gttcatgact gccggcgtta attcccaaga
      481 catttcagct aaattaccac caacaaacaa accgattaag ggcattaaca catctttgac
      541 caaactggaa acgatttttc cgaaagctcc accgataata acaccaacgg ccaaatccaa
      601 aacatttcct ctttgtataa attgaccaaa ttctttaaga aatttacgca taattcctcc
      661 tttattaaga tgaacaaatt ataccacttt tattatcttt gcacaactta ttaaagattc
      721 aagttttctt tcttttatta agtttgattg tataaaatag taaatgtgta aattagtcct
      781 aagcaaaaaa caccatcctt agatggtgtc ttccatatcg ctttcaatga tttgattatc
      841 attgttcttc ttatacatcg acgcttgggt agaaaataag tgataatagc gttgctgtag
      901 attaattaat tgttcgtgag tgccactttc aacgatttgc ccatgttcaa ttaaaatgat
      961 gtggtcaaca tcaaccactg ttgataggcg gtgggcgatg atgatgatgg ttttattagc
     1021 cgcattggtg atgataagat cgttgatggc tttttcggaa taaggatcaa gcgcagatgt
     1081 tggttcatcc aaaatataaa tttgggcatc ggaagcgaaa acacgggcaa tcgctaattt
     1141 ttggcgatca ccgccactaa aaacgacgcc gtcttgaacg aattctctag ttaccaaggt
     1201 atttaaacca tcgggtaaat tctctacaaa atttcttaag ccgaccttat ctaaagcttc
     1261 ccaaactaaa ttttcatctt ctaaagtttt aatttctcgc attaagacat tttcaccaat
     1321 ggtgacggca taaatttgaa aatcctgaaa gacaattgaa aattgttttc gtaactcgtc
     1381 ttctgccaaa tcccggtaat cataacgatt aaagaaca
//
LOCUS       DAOSGN010000279         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_96556_length_1412_cov_2.684598, whole genome shotgun sequence.
ACCESSION   DAOSGN010000279 DAOSGN010000000
VERSION     DAOSGN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96556_length_1412_cov_2.684598"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /gene="argS"
                     /locus_tag="PK087_04100"
     CDS             complement(<1..398)
                     /gene="argS"
                     /locus_tag="PK087_04100"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOH18486.1"
                     /translation="MINTILNNIKLAITKIISAYNLEIDEEIFFEKPKDPSFGDYATN
                     IAMRLAKSLRRSPLIIAKDIVEKIDYPRLHISKVEVAGSGFINFFIDRLYLASVIFSI
                     ITENEHFGDLQLGRGEKINLEFVSANPTGSL"
     gene            complement(422..1411)
                     /locus_tag="PK087_04105"
     CDS             complement(422..1411)
                     /locus_tag="PK087_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003213322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOH18487.1"
                     /translation="MLLPTEISQVAIVWESSHPQVVDNKGTVFRNRDDQTATLTAHLT
                     YKNYQKQVSFSLIVKKMTFELDPYYRGAEGLTKTALKNFLHDLIDDHQEYSYSFAKTA
                     LQKTDEDPNNPNNIILFYTGRSQAKTTYNSGNDGWNREHVWAKSHGGFGDNPPAGTDL
                     HHLRPTDMTVNSIRGNLDFDNGGKKIDETYGAGSSFCYYDNDSFEPRDQVKGDVARIL
                     FYMAVRYDGSDGVADLELNDFTGNGSVPFMGKLSTLLVWNNLDPVDDFERNRNEVIFG
                     YQKNRNPFIDYPHFADLIWGTNSVFTSNYNNGEAVIIQLFVIDWLDFKKQKYC"
BASE COUNT          471 a          286 c          228 g          427 t
ORIGIN      
        1 agtgagccag ttgggttagc actaacaaac tctaaattga ttttttcacc tcgacctaat
       61 tgtaaatcac caaaatgttc attttccgta ataatggaaa aaatcaccga agcaagatat
      121 aaacgatcaa taaagaagtt aataaagcca ctaccagcga cttcaacttt agaaatatgc
      181 aatctagggt aatcaatttt ctcaacaata tccttagcaa ttattaaagg gctacggcgg
      241 agcgatttcg ccaaacgcat ggcaatatta gtagcataat caccaaaact aggatccttc
      301 ggtttttcaa aaaatatttc ttcatctatt tctaaattgt acgctgaaat aatctttgta
      361 attgctagtt taatattatt taaaatcgta tttatcatta attaccactc atttcctcaa
      421 attaacaata tttttgcttt ttaaaatcaa gccaatcaat tacaaacaat tggataataa
      481 ctgcttcccc attgttataa tttgatgtaa aaacactatt tgttccccaa atcaagtccg
      541 caaaatgagg ataatcaata aagggattcc ggtttttctg ataaccaaaa atgacttcat
      601 tgcgatttcg ttcaaaatcg tcaacgggat ccaaattatt ccaaacaagc aaagttgata
      661 atttacccat aaaaggaaca ctaccattgc cggtaaagtc gttaagttcc aagtcagcaa
      721 caccatcact gccatcataa cgaacggcca tgtaaaataa aatccgggcc acatcgcctt
      781 taacttgatc gcgaggttcg aaagaatcgt tatcgtaata gcaaaacgaa gagcccgcac
      841 cataagtttc gtctattttt ttgccaccat tatcaaaatc aagattacca cgaatgctgt
      901 taaccgtcat atccgttggt cgaagatgat gcaaatccgt tcccgcaggc gggttatcgc
      961 caaaaccacc atgagatttt gcccaaacat gttcccgatt ccaaccatcg tttcccgaat
     1021 tataagtcgt tttggcttgt gaacgaccag tgtaaaacaa aatgatatta ttcgggttat
     1081 tggggtcttc gtcagttttt tgcaaagcgg ttttagcaaa actataacta tattcttgat
     1141 gatcatcaat taaatcatgt aaaaagtttt ttaaggccgt tttggttaaa ccctcggctc
     1201 cacgataata aggatctaat tcgaaagtca tcttcttaac aattagagaa aatgaaacct
     1261 gcttttgata gtttttatat gttaagtggg cggtaagcgt cgctgtttga tcgtcacgat
     1321 tacgaaaaac cgtcccctta ttatcaacaa cttgcggatg acttgattcc caaacgattg
     1381 cgacctggga aatttccgtt ggtaaaagca ag
//
LOCUS       DAOSGN010000280         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_96637_length_1411_cov_2.792035, whole genome shotgun sequence.
ACCESSION   DAOSGN010000280 DAOSGN010000000
VERSION     DAOSGN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96637_length_1411_cov_2.792035"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1411
                     /locus_tag="PK087_04110"
     CDS             <1..>1411
                     /locus_tag="PK087_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH18488.1"
                     /translation="NKGKDRPSDLVPGEDDLAISSLTGFNPIFTSYQSPAGDEYYKYN
                     QGTATDPSFVYINVKDENDILTANDIINFDQLERTGNIYRNNDYTKFYVEVLVNKNGV
                     LSPLRYDNIINTTERIMKDDGEAANLSKIAKVSDALFYSETSVYGFFNPEGRGGKYRS
                     TVFSKYLSQYFVDFQKVYKTNTPEDFAKFKLRVYFEMNPEVKQNFEKTYDNFFNDRAG
                     FFLKGIWSIITLGTAPKNDYYQIDKLSKAIDVADIGDSVLTNLPIQGHISSYLLIRGL
                     ISTLLLLYFVIIYLWNIHDAYKTSYEYANNHVFVKDREYFKKMYESSFEYIVLMPALF
                     VLTFISIMPILFGFLIAFTSYNGNSADVGLFDWVGFKNFVLIFNFGSNVGIPFGRIFW
                     RVFLWTVIWAIFSTATCFFGGFFQAVIINSERVPLKKFWRTLLILPWAVPAIISQMVF
                     ANFFNESGVVNAFLQRLGVY"
BASE COUNT          389 a          247 c          274 g          501 t
ORIGIN      
        1 caataagggt aaggaccgtc cttcggattt agttcccggt gaagatgatc ttgccattag
       61 ttcattaaca ggcttcaatc cgattttcac aagctatcaa tcaccggccg gggatgaata
      121 ttataaatat aaccaaggga cagcgactga cccgagtttt gtttacatca atgttaagga
      181 tgaaaatgat atcttaacgg ctaatgatat tattaatttt gatcaacttg aacggactgg
      241 gaatatctat cgtaataatg attatacaaa attttatgtt gaggttttgg ttaataaaaa
      301 cggtgtttta agcccccttc gttatgataa tatcattaat acgacggaac gaattatgaa
      361 agacgacggg gaagcggcta atttaagcaa aattgcgaaa gtttccgatg ctttattcta
      421 tagtgagaca agtgtttacg gtttctttaa tccggaaggt cggggtggaa aatatcgttc
      481 cacggttttt tcgaagtatt tatcgcaata ttttgttgac tttcaaaagg tctataaaac
      541 caataccccg gaagattttg caaaattcaa attgcgagtt tattttgaaa tgaacccgga
      601 agttaaacag aattttgaaa aaacctacga taatttcttt aatgaccgtg ccggtttctt
      661 tcttaaaggc atttggtcaa tcatcacttt agggacagcc cctaaaaatg attattacca
      721 aattgataaa ttatcaaaag cgattgatgt tgctgatatc ggtgattcgg tattaaccaa
      781 tttgccgatt caagggcata tttcatccta tttgttgatt cgtggtttaa tttccacatt
      841 attactacta tattttgtga ttatttatct ttggaatatc catgatgctt ataaaactag
      901 ctacgaatat gccaataatc atgtgtttgt gaaagaccgt gaatatttta agaagatgta
      961 tgaatcttcc ttcgaatata tcgtcttaat gccggccctc tttgtgctaa cctttatttc
     1021 cattatgccg attttatttg gttttttaat tgcctttact tcctataacg gtaattccgc
     1081 cgatgtcggc ttattcgatt gggttggctt caaaaatttt gtcttaatct ttaatttcgg
     1141 ctccaatgtc ggcattccct ttggaagaat cttttggcgg gtcttcttat ggacagttat
     1201 ttgggctatc ttttcgacgg caacttgttt ctttggcggt tttttccaag ccgttattat
     1261 caattcagaa cgggttcctt tgaagaaatt ctggcggact ttactcattt tgccgtgggc
     1321 ggttccggcc attatttccc agatggtttt tgccaacttc tttaatgaaa gcggggttgt
     1381 caatgccttc ttgcaacgcc taggcgttta t
//
LOCUS       DAOSGN010000281         1407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_97042_length_1407_cov_3.104290, whole genome shotgun sequence.
ACCESSION   DAOSGN010000281 DAOSGN010000000
VERSION     DAOSGN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97042_length_1407_cov_3.104290"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PK087_04115"
     CDS             <1..1046
                     /locus_tag="PK087_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18489.1"
                     /translation="NLSYGYASRTEQFTFIVLLDPATSPVNTWHFASVWTEPIAGEAL
                     DPATPNCFAGAVYRKVVSSEDYWLGIEAVVTIPEFTPDSQRFDDTKLSYYLDNSSLYL
                     GGHANYESDVGIGWSIAYENPLSSTLSHSGIAFRPFWRYITRKEECPNNNCYRNADVR
                     DYEYYYYPGDKIRMSVFCPKRGYLQMRIELLELTKNTKFIHARDAYNLGENFNRIFIT
                     PMFPSEGMGAVKTEFKRVNAIDQVANEGKPTLTTNAKVENAIWHEVYLYRKINEVIYK
                     VPMTENRSAHMRCPLGSNVNGNFSNAFIITYNNVDRTLGGEEVTINPRNGTGKLYNTK
                     AILPKKEDYELAV"
     gene            1050..>1407
                     /locus_tag="PK087_04120"
     CDS             1050..>1407
                     /locus_tag="PK087_04120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH18490.1"
                     /translation="MIKPVLASSVWAWLLKHWNEEFHNSFPLSQRLGNEKVIQANPNY
                     QGDVFTYELKGEVVGVLILKYYLRDDFPKHLYLSLLYVLPTKRHQGVGGQLLNFAIAY
                     AKKHGFRKVITGSDPSC"
BASE COUNT          393 a          284 c          324 g          406 t
ORIGIN      
        1 tcaacttaag ttacggctat gccagtcgga cggagcagtt tacttttatt gtccttcttg
       61 atcccgccac ttcaccggtt aacacttggc atttcgcaag cgtttggacg gagccaattg
      121 ctggtgaagc ccttgatccg gcgactccga attgttttgc aggggcggtt tatcggaaag
      181 tcgtttccag cgaggattat tggctgggaa ttgaagcagt ggtgaccatc ccggaattta
      241 cgcccgattc gcagcgcttc gatgatacga aactaagtta ttatctcgat aacagctcac
      301 tttatttggg tgggcatgcc aattatgaaa gcgatgttgg tataggatgg tcgattgctt
      361 atgaaaaccc tctttcctca acgctttcgc atagcggcat tgcttttcgt ccgttttggc
      421 gttacattac gcggaaagaa gaatgtccga acaacaattg ttaccgtaat gccgatgtaa
      481 gggattatga atattattat tatcccggtg ataaaattcg catgagtgtt ttttgcccta
      541 agcggggata tttacaaatg cgcattgaac tgttggaatt aacgaaaaac acgaagttta
      601 ttcatgctcg cgacgcttat aatcttggtg aaaacttcaa ccggattttc ataacgccga
      661 tgtttccttc ggaaggtatg ggagctgtga agacggaatt taagcgcgtt aacgccattg
      721 accaagtagc caatgaaggc aaaccgacat tgacgacgaa tgcgaaagtt gaaaatgcga
      781 tttggcacga agtgtattta taccgaaaaa tcaacgaggt gatttataaa gtaccgatga
      841 cggaaaatcg cagtgcgcac atgcgttgtc ctttaggcag taatgttaac gggaatttct
      901 ccaatgcctt tattattact tataataatg tcgaccgcac tttaggcggt gaagaagtaa
      961 cgattaaccc ccgtaacggc accgggaaat tatataatac caaagccatt ttgcctaaga
     1021 aagaagatta cgaactggcg gtctaaacca tgattaaacc ggttttggct tcatcagttt
     1081 gggcatggtt gttaaagcat tggaacgaag agtttcataa ctcttttccc ctttcccagc
     1141 gtttggggaa tgaaaaagtt atccaggcca atcccaacta ccaaggcgat gttttcacat
     1201 acgaattaaa aggggaagtt gtcggtgttt tgattcttaa atattattta agagatgatt
     1261 ttcccaagca tctctatctt tccttgttat atgtattacc aacaaagcgc caccaaggcg
     1321 tgggcgggca actgcttaat tttgccatcg cttatgcaaa aaagcacggg tttaggaaag
     1381 tcataaccgg tagtgatccg tcatgct
//
LOCUS       DAOSGN010000282         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_97250_length_1405_cov_2.922963, whole genome shotgun sequence.
ACCESSION   DAOSGN010000282 DAOSGN010000000
VERSION     DAOSGN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97250_length_1405_cov_2.922963"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PK087_04125"
     CDS             complement(<1..389)
                     /locus_tag="PK087_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOH18491.1"
                     /translation="MKLLNVKSEIGKLKKVLVHRPGKELDNLTPKMLEQLLFDDIPWL
                     DMAVSEHNHFAALLQNKGVEVLYLSELVAQTLDTSREIRDLFIKQFITEANVTSETLA
                     SVLTDYLNSFTSTLAMVTFLVRPAVRQV"
     gene            573..968
                     /locus_tag="PK087_04130"
     CDS             573..968
                     /locus_tag="PK087_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOH18492.1"
                     /translation="MAADNIKLDFTNTFNGTMIAPKGQILIGTQEGGMRPYNLLFGAL
                     GACFYATFLSIVEKKRLSFASASVEVSGRHRDDQIGTLEYVQIKITIRKPSDEKQFLR
                     SVELGAKYCSIHETISKVAQMEEVVVFED"
     gene            1049..>1405
                     /locus_tag="PK087_04135"
     CDS             1049..>1405
                     /locus_tag="PK087_04135"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOH18493.1"
                     /translation="MWDSVFSSLNDQPVLKLVLECLLIVVARIIEVSMGTLRIILINK
                     GFRKPGVVLAFFEVLMWVFVASQVIAGINEQPIKAVVYSFGFAAGVYVGSRLENYLAF
                     GQGLVQVISDVRMGTII"
BASE COUNT          406 a          224 c          299 g          476 t
ORIGIN      
        1 acctgcctaa cggccggacg aaccagaaat gtgaccattg ccaaggttga ggtaaacgag
       61 tttaagtaat ccgttaaaac cgatgccagg gtttcactgg tgacattggc ttccgtaata
      121 aattgtttga taaacaaatc ccgaatttct cggctggtgt caagtgtttg ggcgactaat
      181 tccgataagt agaggacttc aaccccttta ttttggagta gtgctgcaaa atgattatgt
      241 tcggaaaccg ccatatccaa ccaaggaatg tcatcaaaga gtaattgttc caacatttta
      301 ggtgttaaat tgtcgagctc cttacccggt cggtgaacaa ggactttttt taatttacca
      361 atctccgatt ttacatttaa taatttcatg ataccgtcct ttctttggtc actttatcct
      421 tttttattat atcataaaag cgttttctta gaaacaggaa aatgattaat cgttttcatc
      481 tccaagcatt ttatgacttt gttcttttat ttatgttata ataaattatg tttttagtga
      541 acgatattat aaaaaaaata aaggagatta atatggcagc agataacatt aaacttgatt
      601 ttaccaatac ttttaacgga acgatgattg ctccaaaagg gcagatttta attggtactc
      661 aagaaggcgg catgcgtcct tacaatttat tattcggtgc tttaggagct tgtttttatg
      721 cgacattctt atcgattgtg gaaaagaaac gcttaagctt tgctagtgca agcgtggaag
      781 tctcgggccg tcatcgtgat gatcagatcg gcaccttaga atatgtgcaa ataaaaatta
      841 cgattcggaa gccgagtgat gaaaaacaat ttttaaggag tgtggaatta ggggccaagt
      901 attgctccat tcatgaaacc atcagcaaag tggcgcaaat ggaagaagtc gttgtttttg
      961 aggattagag aatgctttca ttggttttgg cttgattaaa aataagaata tggtataata
     1021 atcatagtga aatgaaggta atattgacat gtgggatagt gtttttagtt ctttaaatga
     1081 tcaaccagtt ttaaaattag ttttggaatg tctgttaatt gttgttgcca gaatcattga
     1141 agtttcaatg ggcacactac ggattattct tattaataaa ggttttcgca agcccggcgt
     1201 tgtgttagct ttttttgaag ttttgatgtg ggtttttgtg gcgtcacagg ttattgccgg
     1261 catcaacgaa caaccaatta aggctgttgt ctacagcttt ggtttcgctg cgggtgtata
     1321 tgttggttcc cgcttagaaa attatttagc tttcggtcaa ggtttagttc aagtcatttc
     1381 cgatgtgcgc atggggacca tcatt
//
LOCUS       DAOSGN010000283         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_97345_length_1404_cov_3.487769, whole genome shotgun sequence.
ACCESSION   DAOSGN010000283 DAOSGN010000000
VERSION     DAOSGN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97345_length_1404_cov_3.487769"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..>1404
                     /locus_tag="PK087_04140"
     CDS             155..>1404
                     /locus_tag="PK087_04140"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOH18494.1"
                     /translation="MNRSIKLLQLDEDIITKLKDHNICQVNHLINFHYEKEGFSQVET
                     KMIVDAFDLYKQEELTKELRGRVNYFPLEYLDEKYSFGELGLSLRAFNGLTRNQITTV
                     SQILKTIIHFEIYELDHLGAKSIAQIMTIVKDIVAREDLAKYLPIYASLHRFDDFTLT
                     EIGFFEQMVKKLSALGFDTLGKLRFGYLNGTLGTLFNYKTMQSFTRKLAEYFSFTADD
                     DFQFFQLYLVDNCFGKMSWTDILSIIGTKDPLYLEQLKKRLQGCCELLVNEEGVRLPY
                     FREKIKNSSLKEETKAILEARFQGMTLQKIASLFHKTRERIRQIIRDRMAQIPLYLEK
                     ALVKEYNRFVWHPEVFKRVYAIDDFSFNVVKYLGERISFKEKYEYPQAYIQSLMDQGR
                     IQAFSLAGFQEYFPKQFAPKMLIYG"
BASE COUNT          442 a          236 c          275 g          451 t
ORIGIN      
        1 gatcatctcg gtgaaaacat cggcgattta atcaaggaat ggcaagaaga tccgcgccaa
       61 ttaagttaat tatgatgaca tttatcagca ataatatcat tgttttattc gcctaattta
      121 tgtataatgt aagtagaaag agaggtgttt ggatatgaac cgctccatta aactgctgca
      181 actggatgaa gatatcatta caaaattaaa agatcacaac atctgtcagg tcaaccattt
      241 aattaatttc cattatgaaa aagaaggctt ttcccaagtc gaaaccaaga tgattgttga
      301 tgcttttgac ttatataaac aagaagaatt gacaaaagaa ttaaggggaa gggtaaatta
      361 ttttccgttg gaatatttgg atgaaaaata ttcatttggg gaattgggcc tttcccttcg
      421 tgcatttaac ggtttgacgc gcaaccaaat aacaactgtt tcgcaaatct taaaaacaat
      481 tattcatttt gaaatatatg aattagatca tttaggggct aagtcgattg cgcaaatcat
      541 gaccattgtt aaggacattg ttgcccggga agacttagca aaatatttac caatttacgc
      601 ttcccttcat cgctttgatg attttacttt aacggaaatt ggcttttttg agcaaatggt
      661 caaaaaactt tccgcccttg gtttcgatac attggggaaa ctccgttttg gctatctcaa
      721 tgggaccttg gggacattat tcaattataa aacgatgcaa agttttacgc gtaaacttgc
      781 tgaatatttt tcgtttacgg ccgatgatga tttccagttt ttccaattat atcttgtcga
      841 taattgcttt ggtaaaatgt cctggacgga tattttaagc atcatcggaa ctaaagatcc
      901 cctttattta gaacaactta aaaagcgttt acagggatgc tgtgaattgc ttgttaatga
      961 agaaggcgtg cggctaccat attttcggga gaagattaag aacagttctt tgaaagaaga
     1021 aacgaaggct attttggaag ctcgttttca gggcatgacg ctccaaaaaa ttgcttcctt
     1081 attccacaaa acgcgggaac gcatccgcca aattattcgg gaccgcatgg cgcaaatccc
     1141 tttatatttg gaaaaagcct tggttaagga atataatcgc tttgtttggc atccggaagt
     1201 ttttaaacgg gtttatgcca ttgatgattt ttcttttaat gtggttaaat atttaggtga
     1261 aagaatcagt tttaaagaga aatatgaata cccccaagct tatattcagt ccctaatgga
     1321 tcaaggtcgg attcaagctt tttccttagc tggcttccaa gaatattttc cgaaacaatt
     1381 tgctccgaaa atgctgattt atgg
//
LOCUS       DAOSGN010000284         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_97647_length_1402_cov_1.891611, whole genome shotgun sequence.
ACCESSION   DAOSGN010000284 DAOSGN010000000
VERSION     DAOSGN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97647_length_1402_cov_1.891611"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PK087_04145"
     CDS             <1..223
                     /locus_tag="PK087_04145"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559582.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     glyceraldehyde 3-phosphate from fructose 1,6,
                     bisphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HOH18495.1"
                     /translation="KAATLGVTKINIDTDGRLVWTRVHREFFRDHPEGFDLRSPGKVF
                     IAEYAKFIVEKSNYLGSANQLAEVRKLVS"
     gene            344..1291
                     /locus_tag="PK087_04150"
     CDS             344..1291
                     /locus_tag="PK087_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOH18496.1"
                     /translation="MRFEYEDGTLIEEQTIDYGNDAIAPTPKNVLGYRFVGWSSSFSN
                     VVSNLKVVAQYEILSFEVIYLDFDNTILKQETVAYGQNGTAPTEPTRTGYNFIAWDQE
                     FTQVTRNLTVRALYEEEAFGLVYVLDDEVYYVHGYTGTNNEIVIPTTYNNFPVVGIYE
                     KAFLNNDTLSKVTISEGIIEIGAQAFSGCVELATVILPNTLESIGEEAFNACSRLAAI
                     TIGAPTIGVAAFSGCTGLKTIALLNTVESIGGWGFWGNSSLKTLYIPESVVFIGKHAF
                     SWCTNLLTIYTPEANVEHLKTLLAEAENIYVKYTVKARE"
BASE COUNT          417 a          266 c          296 g          423 t
ORIGIN      
        1 aaaagccgca acgctgggtg tgaccaaaat caatattgat accgacggcc ggctggtctg
       61 gacgcgtgta catcgcgaat tcttccgcga tcatcccgaa gggttcgacc tgcgcagccc
      121 cggcaaggtt ttcattgccg aatatgcgaa gttcattgtc gaaaagagca attacctcgg
      181 cagcgccaac caattggcgg aagtgcgcaa actggtatct tagcctaaga aacaaactta
      241 cacaaagatc cggtctgcct ccccgctgcc ggatcttttt ttatggtgat accatagtgc
      301 gtataataca atcataaaaa aatggaaaaa aattacattt tcagtgcgct ttgaatatga
      361 agatggaacc ctaatagaag aacaaacgat tgattatggg aacgatgcca ttgcgcctac
      421 tcctaaaaat gttctcggat atcgttttgt tggctggagt agttcctttt ctaatgttgt
      481 tagcaactta aaggttgttg cccaatatga aatcttaagt tttgaagtta tttaccttga
      541 ctttgataac actatcttaa aacaagaaac cgttgcctat ggtcaaaacg gaaccgcgcc
      601 tacggaacca acaagaaccg gatataactt tattgcttgg gatcaagaat ttactcaagt
      661 tacgagaaac ctaactgtcc gcgctttgta tgaagaagaa gcctttggtt tggtttatgt
      721 ccttgatgat gaagtttatt atgtgcacgg ttatacgggt actaacaacg aaatagtgat
      781 tcctaccact tataataact ttcccgttgt tgggatttat gaaaaagctt ttttaaacaa
      841 tgataccctt agcaaagtca ccattagtga aggaatcatt gagattggcg cacaagcttt
      901 ttccggttgt gtggaacttg ctaccgtaat tcttcctaat actttagaaa gtatcggtga
      961 agaagcgttt aacgcttgtt cgcgtttagc cgcgattaca attggcgctc ccaccattgg
     1021 tgttgccgct ttttccggct gtacgggttt aaaaacgatt gcgcttttaa acaccgtgga
     1081 aagtattggc ggttggggtt tttgggggaa tagcagttta aaaacactct atattccgga
     1141 aagtgtcgtt ttcatcggta aacacgcttt tagttggtgc actaatttat taacgattta
     1201 tactccggaa gccaatgttg agcatttaaa aaccttgtta gccgaagctg aaaacattta
     1261 tgtaaaatat acggtcaaag ctagagagta ataatccggt ttgaggtggg tttcatgaaa
     1321 gatgcttatc ggcatttaaa atggttcttt aaaattgaat ataaaaaatt ctaataattt
     1381 tacattttaa atttcaagac ta
//
LOCUS       DAOSGN010000285         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_97927_length_1399_cov_2.668155, whole genome shotgun sequence.
ACCESSION   DAOSGN010000285 DAOSGN010000000
VERSION     DAOSGN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97927_length_1399_cov_2.668155"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1399)
                     /locus_tag="PK087_04155"
     CDS             complement(<1..>1399)
                     /locus_tag="PK087_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOH18497.1"
                     /translation="DPKKTFDNFGINHLAYYDNVYGANKKMSIPALEEYAKYSIDKGL
                     TMKALLGELRKQLAAIDCLSLYEMELQLSFVLSRIEQNGLLIAVDKLEMVGVELVKKA
                     DHIAQDIYFIAGEDFNINSPKQLGEVLFEKLHLPHGKKNKLGYSTNVDVLEKLAKDYE
                     IAKKVLEYRAYTKLISTYINGLKEAMDGNHFIHPLYKQALTLTGRLSSVEPNIQNMPV
                     RTEEGQVIREFFVSRFPDGAILSADYSQIELRVLAHMADDPVMIAAFSQAFDFHTQTA
                     STIFEVPVEQVSKEMRRVAKAINFGIIYGMSAWGLSETIDISPLEANLFINKYFFTFQ
                     KTKAFLDAVVANVKKDGYTKTILGRRRYIPEAVSENNNLRSFGERTAMNAPIQGSAAD
                     IIKIAMIRIDERMKNEQLKSLMIAQVHDELLFDCPKAEVEIMRGLVKEEMEQAFSLKV
                     KLQAEVNVGKTWAEAK"
BASE COUNT          410 a          314 c          242 g          433 t
ORIGIN      
        1 tttggcttcg gcccaagttt tgccgacatt gacttcggct tgtaatttaa cttttaaaga
       61 aaatgcttgt tccatttctt ccttcacaag tcctctcata atctcaactt cggcttttgg
      121 acaatcaaac aatagttcat catgcacctg cgcaatcata agcgacttta gttgttcgtt
      181 cttcatccgt tcatcaatgc gaatcatcgc tattttaatg atatcggcgg cactgccctg
      241 aatcggggca ttcatcgctg ttcgttcgcc aaaactgcgc aaattattgt tttcagacac
      301 cgcttccgga atatagcgcc ggcgccctaa aatagttttt gtatagccgt cttttttcac
      361 attagccaca acggcatcca aaaaagcctt tgttttttgg aaagtgaaaa aatatttatt
      421 gataaataaa ttcgcttcca aaggtgaaat atcaatggtt tcacttaaac cccaagcact
      481 cattccataa ataatcccga aattaatggc tttcgccacc cgccgcatct ctttagaaac
      541 ttgttcgaca ggaacttcaa aaatcgtact cgccgtttgc gtatgaaaat caaaggcttg
      601 agagaaagca gcaatcatca ccggatcatc agccatatgg gccaaaacgc gaagttcaat
      661 ttgcgaataa tcggcactta aaatcgcacc atcgggaaaa cgggaaacaa agaattcgcg
      721 gatgacttgc ccttcttccg tacgaacggg catattttga atattaggtt caaccgatga
      781 taatcgcccc gtcaatgtca aggcttgttt ataaagcgga tgaataaagt gattgccatc
      841 catcgcttct ttcaacccgt taatataggt agaaatgagt ttagtgtaag cgcgatattc
      901 caaaactttt ttcgcaatct cataatcctt ggctagtttt tctaaaacat cgacattggt
      961 tgaataaccc aatttgttct ttttgccgtg aggtaaatgt aatttttcaa acaatacttc
     1021 acctaattgc tttggtgaat tgatgttaaa gtcctcgccg gcaataaaat agatgtcttg
     1081 cgcaatatga tcagcttttt tcacaagttc cacgccgacc atttccaatt tatccacagc
     1141 aatcaataaa ccattttgtt cgatacgcga caaaacaaag gataattgta attccatttc
     1201 ataaagcgaa agacagtcaa tggccgctaa ttgcttcctt agttccccca gcaaagcttt
     1261 catcgtcaag cctttatcaa tgctgtattt cgcatactct tctagggcgg gaatgctcat
     1321 ttttttatta gccccataga cattatcata ataggctaaa tggttaatgc caaagttatc
     1381 aaaggttttt ttcggatca
//
LOCUS       DAOSGN010000286         1393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_98580_length_1393_cov_2.171151, whole genome shotgun sequence.
ACCESSION   DAOSGN010000286 DAOSGN010000000
VERSION     DAOSGN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98580_length_1393_cov_2.171151"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="der"
                     /locus_tag="PK087_04160"
     CDS             <1..1188
                     /gene="der"
                     /locus_tag="PK087_04160"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOH18498.1"
                     /translation="IYSKASWLNQDFRVIDTGGIILKDEPFSKEIRAQAELAIDEADV
                     IVMVVDARSGLTVEDEDVISLLQKSHKPLIVAANKVDDNRYVDMIYDFYSLGVSEVLP
                     ISTLHGIGIGNLLDRLIELLPVVKKDQYEQTTIKFSLIGQPNVGKSTLANAILGEERT
                     IVSDIPGTTRDSIDTIFTRNNEKYVVIDTAGIRKAGKIYENAEKYSLFRALQAIERSD
                     IAVVILDGSQPITEQDKRIAGYAREYNRGIIICVNKWDLVVKDDKTMKEFTEKIRKEF
                     LFLDFAPVVFLSAKNNARIHLLLDEIDHVYRNFTKRIPTNLLNDCLLDATLLNQPPVY
                     KGNRLKLSYATQEETMPPSFVLFVNSEKHMHFSYLRYLENQIRATFDFQGSPLKFTLR
                     KKE"
     gene            1188..>1393
                     /locus_tag="PK087_04165"
     CDS             1188..>1393
                     /locus_tag="PK087_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOH18499.1"
                     /translation="MEKLVIIGAGSWGSGLARIIGDNGYEIMMYDINQKQVDEINQFH
                     TNRAKLPVGVLNESVRATSNLKEA"
BASE COUNT          458 a          231 c          284 g          420 t
ORIGIN      
        1 atttatagca aagcttcatg gttaaatcaa gatttcagag taattgatac tggagggatc
       61 atcttaaaag atgagccctt ttctaaagaa atccgggcac aagccgaatt ggcaattgat
      121 gaagctgatg tcatcgtcat ggttgtcgat gctcgcagtg gtttaacggt tgaagatgaa
      181 gatgttattt cgctattaca aaaaagtcat aagccgctaa ttgttgcggc taacaaagtt
      241 gatgataatc gctatgtaga tatgatttat gatttttata gtttaggagt ttcagaggtg
      301 ctccccatta gcactttaca tgggattggg attggtaatt tacttgatcg tttaattgaa
      361 ttacttccgg ttgtgaagaa agatcaatac gaacaaacaa cgattaaatt ttccttaatc
      421 gggcaaccga atgttggaaa atcgacctta gccaatgcca ttcttggtga agagcgcaca
      481 attgtcagtg acattcccgg aacaactcga gattccatcg ataccatatt tacgcgaaat
      541 aatgaaaaat atgtggttat cgatacggca ggaattcgta aagcggggaa aatttatgag
      601 aatgcggaaa aatatagcct atttcgtgct ttacaagcta ttgaacgaag cgatattgcc
      661 gttgttattc tcgatggatc gcaaccgata acagaacagg ataagcgaat tgccggttat
      721 gcccgggaat ataatcgcgg aattatcatt tgtgttaaca aatgggattt agttgtaaaa
      781 gacgataaaa cgatgaaaga atttacggaa aaaatacgca aagaattttt atttttagat
      841 tttgcgcccg tggtttttct ctcggccaaa aataatgcgc ggattcattt gttgcttgat
      901 gaaattgacc atgtttatcg taatttcaca aagcgcatac cgaccaattt acttaatgat
      961 tgtttgcttg atgcgaccct cttaaatcaa ccgccggttt ataaaggaaa tcgtttaaaa
     1021 ttatcttatg ccacccaaga agaaacgatg ccgccttcat ttgttttatt cgttaatagt
     1081 gaaaaacata tgcatttttc ttatttacgc tatttagaaa atcaaattag agcaacattt
     1141 gattttcaag gttcacccct caaattcact ttaaggaaaa aagaataatg gaaaaattag
     1201 taattattgg cgccggcagc tggggcagcg gtttagctcg aattatcggc gataatggtt
     1261 atgaaataat gatgtacgac attaatcaaa aacaagttga tgaaatcaat cagtttcata
     1321 ccaaccgtgc caaattaccg gtaggtgttt taaacgaaag tgttcgtgca acaagcaatc
     1381 taaaagaagc cat
//
LOCUS       DAOSGN010000287         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_99386_length_1386_cov_2.034560, whole genome shotgun sequence.
ACCESSION   DAOSGN010000287 DAOSGN010000000
VERSION     DAOSGN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99386_length_1386_cov_2.034560"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /gene="nadE"
                     /locus_tag="PK087_04170"
     CDS             <1..851
                     /gene="nadE"
                     /locus_tag="PK087_04170"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOH18500.1"
                     /translation="YVGVTKTIIGVSGGLDSTLALLSLCYAYDRYQLKRQDIIAVRMP
                     SKENSPTSNQNAQELVDKLGVTSLVIPIQEDVNRQLAILKHDLENKDTTYENVQARFR
                     TYTLMNLANHHKGLVVGTSDMSEIALGWSTFNGDHMAMYGLNAGITKTVVIETVRFYK
                     TIFPEVRKILDLIIDAPISPELSGSKQRTEDVIGKFAINDFILYHFLVDGDDAERISF
                     LLTKAFQMDIKKAQAAVDNFNKRFYSQQYKRLTMPEGAKVLGISLSPRTELKLNGDIY
                     RIKVEE"
     misc_binding    890..1094
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1132..>1386
                     /locus_tag="PK087_04175"
     CDS             1132..>1386
                     /locus_tag="PK087_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH18501.1"
                     /translation="MKLKDLPIKYDFQKVEQGKYQKWLKKGFFTAGDKDKIPFTIVIP
                     PPNVTGKLHLGHVLDTTMQDIIIRRKRMQGFDVLYLPGMDH"
BASE COUNT          446 a          219 c          292 g          429 t
ORIGIN      
        1 attatgttgg cgtcactaaa acgataattg gtgttagtgg cggccttgac tcgactttag
       61 ccttgttatc tttatgttat gcctatgatc gctatcaatt aaaacgccag gatattattg
      121 ctgttcgcat gccttcaaaa gaaaacagtc ccactagcaa tcaaaatgct caagaattag
      181 ttgataaatt gggggtgacg agcttagtca ttcccattca agaagatgtc aatcgccaac
      241 tggcgattct caagcatgat ttggaaaata aagatacgac ttatgaaaat gttcaagcac
      301 gttttcgaac ttatacttta atgaatcttg ctaaccatca taaaggtctt gttgttggga
      361 cttctgatat gagtgaaatt gcgctagggt ggtcgacctt taatggtgat catatggcaa
      421 tgtatggctt aaatgccgga attactaaaa cggtagtgat tgaaaccgtc cgtttttata
      481 aaacgatttt tccggaagtt cgcaaaatct tggatttaat tattgatgca ccgatttcac
      541 cggaattatc gggaagtaag caaaggactg aagatgtaat tggcaaattc gcgattaatg
      601 actttatcct gtatcacttt ttagttgatg gtgatgatgc cgaacgcatt agctttttgc
      661 taaccaaagc cttccaaatg gatatcaaga aggcacaagc ggctgttgat aattttaata
      721 agcgctttta ttctcaacaa tataaacgtt taacgatgcc ggaaggggct aaagtattgg
      781 gaatatcttt atcgccaagg acggaattaa aacttaatgg cgatatttac cgaattaaag
      841 ttgaagaata gcttagttgt tgacttttaa ataatgaagt tatataattt ataaaacgaa
      901 gaagaggaag agtaagagct tacttttgat ttagagaagg acggtcggtg agaagtccaa
      961 tcaagtaact ttgaaggtcg ccttggagtt gatgagatga actttagtag tcctgtccgt
     1021 attcctgcgt taaaggtaat gagagcaatg taattgttga aataaggtgg taccacggtt
     1081 aattcgtcct taagtgaaag cttaaggact ttttttgaag ggagattaga aatgaaatta
     1141 aaagatttac caattaagta tgattttcaa aaagttgaac aaggtaaata tcaaaaatgg
     1201 cttaaaaaag gattttttac tgcaggtgac aaagataaaa ttccgtttac gattgtcatt
     1261 ccgcccccca atgttacggg taaactccat ttaggtcatg ttttagatac aaccatgcaa
     1321 gatatcatta ttcgccgcaa acgaatgcaa ggcttcgatg tcctttatct tccaggaatg
     1381 gatcat
//
LOCUS       DAOSGN010000288         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_99527_length_1384_cov_3.437923, whole genome shotgun sequence.
ACCESSION   DAOSGN010000288 DAOSGN010000000
VERSION     DAOSGN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99527_length_1384_cov_3.437923"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1384
                     /locus_tag="PK087_04180"
     CDS             <1..>1384
                     /locus_tag="PK087_04180"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH18502.1"
                     /translation="LGVNIVRTSHYPVSEHFLNACDELGLLVMEEIPGWQHLGGEQFQ
                     NNVLKNTQAMITRDRHHPSIILWGVRINESADLDSLYRETNKLAKKLDPTRPTGGVRN
                     FANSHMFEDVYTFNDFTHAGKKAALQKASKIKKGKPYLVTEHNGHMFPTKPFDDEKHR
                     VSQALRHEAVLKAAQKQAQIAGCIGWCMNDYQTHPQFGSGDKICYHGVLDMFRLPKYA
                     AFLYAAQQDKQAVLEVLSTLNPGDYPKSYLNEVYIATNCDCVKIYKDDYFLGEYHPNK
                     PTAFLKPLLKVTDFIGNYLVEKYHFTKFDARLAKQIFKQIQKNPNLPFIFRLKMLYIM
                     LKYRLSRQKATEVFYQLTGGANTFRFEAYQKGTLVKQVIKEKQTSSKWLLEADDDKLV
                     IAETYDVVRVVVKKVDQNHQVLPYAFDAFTISVQGGISLIGPALIALRAGQAAFWVRT
                     KGKAQDGKITI"
BASE COUNT          434 a          273 c          282 g          395 t
ORIGIN      
        1 attgggagtt aacatcgtcc gtacttccca ttatccggtt tccgaacatt ttttaaatgc
       61 ttgtgatgaa ctgggtttgt tggtaatgga agaaattccc ggatggcaac atcttggcgg
      121 cgaacaattc caaaacaatg ttttaaaaaa cacacaggcg atgattactc gtgatcgcca
      181 tcacccttct attattttgt ggggggtgcg gattaatgaa tccgccgatt tagattcttt
      241 atatcgagaa acaaacaaac tggcgaaaaa actcgaccct accagaccaa cagggggagt
      301 ccgcaacttt gctaacagcc acatgtttga agatgtttat actttcaatg attttacgca
      361 tgcagggaaa aaagcggccc tccaaaaagc atccaaaata aaaaagggaa aaccgtattt
      421 agtgaccgaa cataatggtc atatgttccc aacaaaacct ttcgatgatg aaaaacatcg
      481 cgtcagccaa gctttaaggc atgaagcagt tttaaaagcg gctcaaaaac aagcccaaat
      541 tgccggttgc atcggttggt gcatgaatga ttatcaaacc catcctcagt ttggcagcgg
      601 tgacaaaatt tgctatcacg gtgtgttaga tatgttccgc ctccccaaat atgcggcttt
      661 tttgtatgcc gcccaacagg ataaacaagc agttttggaa gtgttatcga cccttaatcc
      721 cggtgattat ccgaaaagct atttaaatga agtctatatt gccacaaatt gtgattgtgt
      781 gaaaatctat aaggatgatt attttcttgg tgaatatcat ccaaataagc cgacagcgtt
      841 tttaaaaccc ttattgaaag taacggattt tatcggtaat tatttagttg aaaagtacca
      901 ttttacgaaa tttgatgcgc gcttagcgaa gcaaattttc aaacaaattc aaaaaaatcc
      961 caatttgccg tttattttcc gccttaaaat gttatatatt atgcttaaat atcgcctttc
     1021 ccgtcaaaag gcgaccgaag tcttttatca actaactggt ggagctaata ctttccgctt
     1081 tgaagcttat caaaaaggga ctttggttaa acaagtcatt aaagaaaaac aaacaagcag
     1141 taagtggctt ttagaagctg atgatgataa acttgttatt gcggaaactt atgatgtcgt
     1201 gcgcgtggtt gtgaaaaaag ttgatcaaaa tcatcaggtt ctcccctatg cttttgatgc
     1261 tttcacaatc tcggttcaag gcggaatcag tttaatcggg ccggcgctta tcgctttaag
     1321 agccggacaa gcggcttttt gggtgcggac caagggtaaa gcccaagacg gaaaaatcac
     1381 catt
//
LOCUS       DAOSGN010000289         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_99547_length_1384_cov_2.738149, whole genome shotgun sequence.
ACCESSION   DAOSGN010000289 DAOSGN010000000
VERSION     DAOSGN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99547_length_1384_cov_2.738149"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PK087_04185"
     CDS             <1..1077
                     /locus_tag="PK087_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18503.1"
                     /translation="LQEFQEKNKQLKKSSLKQIFIFGIFRPSIYVLYIATVMLILWFG
                     SNNVILQAGLTFEVIIIFHQYIDRLFNPIQQLAEQFNILQSAFAASEKIFAVLDQQPE
                     IVDAPDAIELSDVKGEIEFKNVWFSYLPDEWVLKDVSFKIKANETVAFVGATGSGKTT
                     ILSLIVRNYDIQKGQILIDGIDIRKIKIASLRSQIGQMLQDVFLFSGTINSNIKMRED
                     SITDDDVKQACEYVNADKFINSLPNAYQEEVRERGNNFSAGQRQLLSFARTMAHQPKV
                     MILDEATANIDTETEVLIQDSLAKMMNVGTMLVVAHRLSTIQHADKIIVLQKGEIVEE
                     GNHQELLKKRGQYYKLYQLQYQHQ"
     gene            complement(1118..>1384)
                     /locus_tag="PK087_04190"
     CDS             complement(1118..>1384)
                     /locus_tag="PK087_04190"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOH18504.1"
                     /translation="DKTAWSRFKANLIITVVGHKYRHLKSSSLQSLERGQLTEIDYFN
                     GYIVRKADEIGIDVPINRQLVAMIKKIEAKQLPIQLSNLDDVQI"
BASE COUNT          432 a          267 c          266 g          419 t
ORIGIN      
        1 ctccaagaat ttcaagagaa gaacaagcaa ttaaaaaaga gctctttaaa acaaatcttt
       61 atttttggga ttttccggcc ctccatctat gttctttaca ttgcgaccgt gatgttaatt
      121 ctttggtttg gtagcaataa tgttatcctg caagccggcc ttacctttga agttattatt
      181 atctttcatc agtacatcga tcgtttgttc aacccgattc aacaattagc ggaacaattc
      241 aacattttgc agtcggcatt tgcggcctcc gaaaaaatct ttgcggtttt ggatcaacaa
      301 ccggaaatcg ttgatgctcc cgacgccatc gaactaagcg atgtcaaggg cgagattgag
      361 tttaaaaatg tttggttttc ttatcttccc gatgaatggg tcttaaaaga tgtctctttt
      421 aaaatcaaag ccaatgaaac cgtggcattt gtgggggcga ccggcagcgg caaaaccacg
      481 attctttcct taattgttcg caattacgat attcaaaaag gccaaatcct aattgatggt
      541 attgatattc gaaaaattaa aattgcttca ctccgttccc aaattggaca aatgttgcaa
      601 gatgtcttct tgttcagtgg gaccattaac tcaaatatca aaatgcggga agacagcatt
      661 accgatgatg atgttaaaca agcctgtgaa tatgttaatg ctgacaaatt tattaacagc
      721 ctgcccaatg cttatcaaga agaagttaga gaacgaggaa acaatttttc agccgggcaa
      781 aggcaattat tatcctttgc gagaaccatg gctcatcaac ccaaagtgat gattcttgat
      841 gaagcaacag cgaacattga tacggaaacg gaagttctca ttcaagattc cttagcaaaa
      901 atgatgaatg tcggaacaat gttggtggta gcgcatcgct tatcaaccat tcagcatgcg
      961 gacaaaatta ttgtcctgca aaaaggtgag attgtggaag aaggcaatca ccaagagttg
     1021 ttgaaaaaac gtggtcaata ttataaacta tatcaacttc aatatcagca tcaataaaaa
     1081 aagagcaatc atagttcaat ctatgttgct cttgttttca tatttgcaca tcgtctaaat
     1141 tgcttaattg aataggcagt tgtttggctt caatcttttt aatcatggct actaattgcc
     1201 gattaatcgg aacatcaatc ccgatttcat cggcctttct tacaatataa ccgttgaagt
     1261 aatcaatttc cgttagttgt ccgcgttcta aggattggag actggaagac tttaaatggc
     1321 ggtatttgtg accgaccacg gtaataatca aattggcttt aaaccgactc catgctgttt
     1381 tatc
//
LOCUS       DAOSGN010000290         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_99873_length_1381_cov_3.384615, whole genome shotgun sequence.
ACCESSION   DAOSGN010000290 DAOSGN010000000
VERSION     DAOSGN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99873_length_1381_cov_3.384615"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PK087_04195"
     CDS             complement(<1..546)
                     /locus_tag="PK087_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH18505.1"
                     /translation="MGILFSKVAFAYYRPRKKQYLRYILEDINLNIAEQDEMIAIVGH
                     TGSGKSTLVQMMNALLLPTAGEVTLFKTKITPTVKLKPIRQKVGLVFQFPEYQIFEEN
                     VKKEIMFGPKNFKMPNLEQTALEAAQVMGITDLLERSPFTLSGGQLRKVAIASILATN
                     PDVLILDEPTVGLDPLTKRELL"
     gene            complement(522..1325)
                     /locus_tag="PK087_04200"
     CDS             complement(522..1325)
                     /locus_tag="PK087_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOH18506.1"
                     /translation="MIKVEHINFSYRPNEPVIRDVSFTIKKGEFVCILGHNGSGKSTI
                     AKLLTGLLSVDSGAIYIKGLLLNENNINEIRQQVGIVFQNPDNQFVGVTVKDDIAFGL
                     ENRQFPRETMLELIDKYSKLVNMENFLDYNPENLSGGEKQRVAIAGVLAANPEIIIFD
                     ESTSMLDPKGVKEVTDIINTLKGHKTIIAITHNLNEAVFADRVIVLSQGGVILNDTPE
                     VVFTHKELLKNSSLDILESMKLMELIEKNEHLKNKQEIEAMLWEYSFQK"
BASE COUNT          408 a          285 c          252 g          436 t
ORIGIN      
        1 gagtaattcc cgtttagtta aaggatccaa acccactgtc ggttcatcta aaatgagtac
       61 atccggattg gttgctaaaa tggaagcaat cgccacctta cgaagttgcc cacccgacaa
      121 cgtaaagggg ctgcgctcca ataagtcggt aatgcccata acttgggctg cttcaagagc
      181 tgtttgttcc aaattcggca ttttaaagtt tttcggtcca aacatgattt ctttttttac
      241 attttcctca aaaatctgat attccggaaa ctgaaaaacc aaaccgacct tttggcgaat
      301 cggttttaat tttactgtcg gcgtgatttt agttttaaaa agcgtcactt cgcccgcagt
      361 cggcaaaagc aaagcgttca tcatttgcac caaagttgat ttaccgctgc cggtatgacc
      421 gacaatggca atcatttcat cttgttcggc aatgtttaaa ttgatatctt caagaatata
      481 acgaagatat tgtttcttac gaggtcggta ataggcaaaa gctacttttg aaaagagtat
      541 tcccataaca tcgcctcgat ttcctgttta ttttttaaat gctcattttt ttcaattaat
      601 tccattaatt tcatgctttc caaaatatcc aaagaactgt tttttaggag ttctttgtga
      661 gtaaaaacaa cttcaggagt atcatttaaa ataactccac cttgtgataa gacaatgaca
      721 cggtccgcaa aaacggcttc gtttaaattg tgagtaatgg caataatcgt tttgtgacct
      781 ttgagggtgt tgatgatgtc ggtgacttct ttgaccccct tgggatcaag catcgatgtg
      841 gattcatcaa aaatgataat ttccggattg gcagccaaca ctccggcaat cgcaactctt
      901 tgtttttcgc cacccgataa attttccgga ttataatcca aaaaattttc catattgact
      961 aatttagaat acttatcaat taactcaagc atcgtttcac gcggaaattg gcggttttcc
     1021 aaaccgaaag caatatcgtc tttgacggtt acaccgacaa attgattgtc cggattttgg
     1081 aaaacaatgc cgacctgctg gcgaatttcg ttaatattgt tctcattgag caataagccc
     1141 ttaatataaa tcgcaccgct gtcaaccgaa agaagtccgg ttaacaattt cgcaatcgtc
     1201 gatttcccgc tgccgttatg gccaagaatg caaacaaact cgcctttttt aatggtaaaa
     1261 gaaacatcac gaataaccgg ttcgttaggg cgatatgaaa aattaatgtg ttctacttta
     1321 atcattttag acctcgataa tgatttcttt tgtgacataa cgaaccatga tgccgttagt
     1381 a
//
LOCUS       DAOSGN010000291         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_100127_length_1379_cov_2.758308, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000291 DAOSGN010000000
VERSION     DAOSGN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100127_length_1379_cov_2.758308"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PK087_04205"
     CDS             <1..1031
                     /locus_tag="PK087_04205"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH18507.1"
                     /translation="PYEEEVIITQSLVYPNTINRMRPGTGLVGKKMPGGVKYIVIHDT
                     GMSGATGTAVGVKDYIHAQANSVTGRVASWHYTIDDKDCFQHVPDDEIAWHAGDGSSA
                     YGSTYFNKEYQAWSIGGGNQNGIGIETCINQGGNYQRTLQRTAKLVASLLIKHHLGLD
                     AIKQHNDFSGKNCPAVIRSSSGRWSEFLKMVEWHLMMNSFDANYSVKTSISNPSAIGK
                     DGIVKQNLINDENVLLSFDITLGSDVLHYEYHVLAKGMTTSEKFSSLYLYLYNNVIPR
                     ETKESLLLPLWNEDYQAKLSWESSHPELLSNDGHYTPPKTAKLVSLWVTIEMDGQTET
                     KTFKIYVG"
     gene            complement(1192..>1379)
                     /locus_tag="PK087_04210"
     CDS             complement(1192..>1379)
                     /locus_tag="PK087_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18508.1"
                     /translation="LFVYLLLHLFYLVFKDLIIPVFYPKKPYFSGCAFLGQPLVLYYK
                     GNDLSNKIVFILLGASS"
BASE COUNT          425 a          256 c          300 g          398 t
ORIGIN      
        1 tgccctatga agaagaagtt atcattaccc aatcgcttgt ctatccgaat actattaatc
       61 gaatgcgtcc gggtacgggc ttggtgggta agaaaatgcc gggcggtgtc aaatacatcg
      121 ttattcatga tacggggatg agcggtgcca ccggtaccgc tgttggtgtg aaagattata
      181 ttcatgcgca agccaattcg gttaccggcc gcgtcgcttc ttggcattat acgattgatg
      241 ataaagactg cttccaacat gttcccgatg atgagattgc ttggcatgcc ggagacggca
      301 gcagtgccta tggctccact tatttcaata aagaatatca agcttggtcc atcggtggtg
      361 gcaatcaaaa cggcattggc attgaaacct gcatcaacca aggcggcaat tatcaacgga
      421 ctttgcaaag gacggcgaag ttagttgcct cattattaat caaacatcat ttaggtttgg
      481 atgccattaa acagcataac gacttttccg ggaaaaattg ccccgccgtc atcagaagtt
      541 ccagtgggcg ctggagtgag ttcttgaaaa tggtggaatg gcatttaatg atgaacagtt
      601 ttgatgccaa ttattctgtg aaaacaagca ttagtaatcc ttcggctatt gggaaagacg
      661 gtatagttaa gcaaaacctt attaacgatg aaaatgtttt gcttagtttt gatattactt
      721 taggaagtga tgtcctccat tatgaatatc atgttcttgc gaaagggatg acaacaagcg
      781 aaaagttctc gagtttatat ctctatttat ataataatgt tatacctcga gaaacaaaag
      841 agtcgctgtt attgccgctt tggaacgaag attatcaagc aaagctctca tgggagtcga
      901 gccatcccga actattatcc aacgacggtc actatacacc cccgaagaca gctaagcttg
      961 tttcgctttg ggtcaccatt gaaatggatg gccaaacaga aaccaaaaca tttaaaattt
     1021 atgttggttg ataccatcta aagccgccaa ggcggctttt ttggtgtaaa agcgttgact
     1081 tttaattttg aattaggtat aatatccaag caattatgaa gaattaggga attgatagtt
     1141 tctagacggt gttttcacaa tcgataattt cccatttttt tatgtttttg tttaggaaga
     1201 tgcccccaac aaaataaaca cgattttgtt tgacaaatca tttcctttgt agtataatac
     1261 caaaggttgc cccaaaaatg cgcaaccgct aaaatacggt ttttttggat aaaaaacagg
     1321 aataataagg tctttgaaaa ctaaatagaa caggtggagc aggagataaa caaataatg
//
LOCUS       DAOSGN010000292         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_100676_length_1374_cov_3.214556, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000292 DAOSGN010000000
VERSION     DAOSGN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100676_length_1374_cov_3.214556"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1057)
                     /locus_tag="PK087_04215"
     CDS             complement(104..1057)
                     /locus_tag="PK087_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HOH18509.1"
                     /translation="MKKRNTFWIMIGIGVVIIILLILVSSILAVGERLRTISPYLEYT
                     FYALAILLVYFLIINPIRIILFAPAFSLTAVLDGQEKNSRRVKKRITRKLIKSEEVKA
                     EDKKLLVDSLKDQTQLDEQLKIYFQKTLKQEINKIVLRNAKTVMISTAISQNGRLDLI
                     TVLVVNLKMIKEIVLKSGFRPSYAKLGKLSANVLGTALIAEGLEGLDFNDIFPASTTN
                     FLSEIPLIKPLASSIIQGISNALLTIRVGIITRKYLYAEYKNYSKNEVRRDSIKESIK
                     LLPSVIKDAITFLPNRIAKLFQKRKLTPEEQAVYDALQSEA"
     gene            complement(1120..>1374)
                     /locus_tag="PK087_04220"
     CDS             complement(1120..>1374)
                     /locus_tag="PK087_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18510.1"
                     /translation="NLLLPRFDYDSDVKAVKQGMSVLMTMLTGFVTVTIVIVAGVLGV
                     VFFTPIVAYIFAFIAASLIAIVFISLVQTHGVKLFNRING"
BASE COUNT          435 a          262 c          234 g          443 t
ORIGIN      
        1 aagtattttt acttttttgt gcacaaagga agttttatct ttcatatcaa taaaaaagca
       61 tcttataatc tcttagcatc aaaagaaaac aatttctttg tctttaagct tcgctttgta
      121 aagcgtcata aacagcctgt tcttccggtg ttagtttgcg tttttggaat aatttcgcaa
      181 tcctattcgg taaaaatgtt atcgcatctt tgataacgga aggtaaaagt ttaatcgatt
      241 ctttgataga atcacgacgg acttcatttt tagagtaatt tttgtattcc gcatacaaat
      301 atttccttgt gataataccg acgcgaatcg ttaacaaggc attggaaata ccttgaatga
      361 tactggaagc taagggttta attaacggga tttctgacaa aaaattggtt gtggaagcgg
      421 gaaaaatatc attgaaatcc agtccttcca agccctccgc aatcaaggcc gtaccaagca
      481 catttgctga taatttcccc agtttcgcat agctcgggcg aaagccgctt tttaggacaa
      541 tttctttaat catttttaaa ttaaccacca aaaccgtaat taaatcaagg cgaccgtttt
      601 gggaaatcgc tgtcgatatc attaccgttt tcgcattccg taaaacaatc ttgttaattt
      661 cttgtttcaa agtcttttgg aaatatattt ttagttgttc atccaattgc gtttggtctt
      721 tcaaactatc aactaataat tttttatctt ccgctttaac ttcttcgctc ttgattaact
      781 ttctagtaat tcgtttttta acccgccgac tatttttttc ttgaccgtcc agcaccgccg
      841 ttaaggaaaa ggccggtgcg aacaaaataa tccgaatcgg attgataatt aaaaaataaa
      901 ctaataggat tgctaaagca taaaaagtat attccaaata gggagagatt gttcttaagc
      961 gttccccaac ggctaaaata ctgcttacta aaataagtaa aatgatgatt acgaccccaa
     1021 tgccaatcat aatccaaaat gtattgcgtt ttttcataat aattctcctt tttggtttat
     1081 tataacataa aaaccactta gtgataagtg gtttttggct tatccgttga tacggttaaa
     1141 taacttgact ccgtgggttt gcactaaact gataaagacg atggcgatta acgaagcggc
     1201 gataaaggcg aagatgtaag ccacaatcgg ggtgaaaaac acgaccccca aaacaccggc
     1261 gacgatgacg atagtaactg ttacaaaccc ggtcaacatc gtcattaaga cggacattcc
     1321 ttgtttaaca gccttcacat cgctatcata atcaaagcgc ggtaataata aatt
//
LOCUS       DAOSGN010000293         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_100888_length_1372_cov_3.074412, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000293 DAOSGN010000000
VERSION     DAOSGN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100888_length_1372_cov_3.074412"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="PK087_04225"
     CDS             <1..156
                     /locus_tag="PK087_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic transporter"
                     /protein_id="HOH18511.1"
                     /translation="TPIGASANIAGIGILRKEGYEVKNSDFFKIGIPFTLAAVITGYL
                     LLWFIWS"
     gene            240..755
                     /locus_tag="PK087_04230"
     CDS             240..755
                     /locus_tag="PK087_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOH18512.1"
                     /translation="MLNEKVTKLLNEQINKEFYSAYLYLDMANFYQNKGLEGFANCFN
                     VQALEEQSHAMLFIKYLQNNGFNVTLDAIAKPHLVYKELKEPLHGALEHEQFVTASIH
                     HIYDVALSLKDYRTIEFLNWFVKEQGEEEMNAENLISAYENFGKHGGSLYALDRELKA
                     RQFVAPSLNLD"
     gene            862..1197
                     /locus_tag="PK087_04235"
     CDS             862..1197
                     /locus_tag="PK087_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18513.1"
                     /translation="MKLLVYVMNNANLLDRFVHELKDAQIRGATILNSTGMGRKLVES
                     DNFDIIGSLKAIFDTPRTDSRVILMALEDEQVPRVLDIIDRLAGDLSKPNSGIVFTLN
                     IEYVKGYKK"
BASE COUNT          429 a          199 c          285 g          459 t
ORIGIN      
        1 acaccgattg gcgcttctgc caacattgcc gggattggga ttttgcggaa agaaggttat
       61 gaagtcaaaa actccgattt ctttaaaatc ggaattcctt tcaccttggc agcagttatc
      121 accggttatt tattgctttg gttcatttgg agctaaaagc atttcagttg aattattaaa
      181 aataaatatg ttataatctt tatgtaagga aaaaccttat agaaaggtga gaaatgaaaa
      241 tgttaaacga aaaagtaaca aaattattaa atgaacaaat aaacaaagaa ttttattcag
      301 cttatttata tttagatatg gctaatttct atcaaaacaa aggtttggaa ggatttgcca
      361 attgtttcaa tgtccaggct ttagaagaac aaagccatgc gatgttgttt atcaaatatt
      421 tacaaaacaa tggttttaat gttacgctgg atgcgattgc gaaaccgcat ttagtttata
      481 aggagttgaa agaacccctt catggtgcct tggaacacga acaatttgtt accgcctcca
      541 ttcatcatat ttatgatgtg gccttgtcct tgaaagatta tcgcaccatt gaattcttaa
      601 actggtttgt gaaagaacag ggtgaggaag aaatgaatgc tgagaattta atttccgctt
      661 acgagaactt tggaaaacat ggcgggagcc tttatgccct tgatcgagaa ttgaaagcgc
      721 gtcagtttgt tgctccatca cttaatttag attaatgttc atattattaa agctaagaaa
      781 tccgttagtt atttcttagc ttttttttat tgctatcaag ctaacatcgg ggtataatga
      841 ataaagacaa gtgaggtaaa tatgaaactg ttagtttatg tgatgaataa tgctaatctt
      901 ttagaccgct ttgttcacga gttgaaagat gcgcaaatta gaggcgccac gattttaaac
      961 agtacgggga tgggtagaaa attagttgaa agcgataact ttgatattat cgggtcctta
     1021 aaagccatct ttgatacgcc aaggacggat tcacgagtta ttttgatggc tttggaagat
     1081 gaacaagtgc caagggtttt agatataatt gatcgtttag ccggtgattt atccaaaccg
     1141 aattctggca ttgtttttac gcttaatatt gagtatgtaa aagggtataa gaagtagcta
     1201 tggatatttt attatatgcg gggattgttt tagtaaccgg acttttgttt ggtaaattag
     1261 ctaaatactc acatttaccg aatgttaccg gttatttagt tggtggttta attattggtc
     1321 ccagtgtcct cggtttggtt ggcgaagatc aactgctttc cttaaatttg gt
//
LOCUS       DAOSGN010000294         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_100905_length_1372_cov_2.747153, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000294 DAOSGN010000000
VERSION     DAOSGN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100905_length_1372_cov_2.747153"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1372)
                     /locus_tag="PK087_04240"
     CDS             complement(<1..>1372)
                     /locus_tag="PK087_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH18514.1"
                     /translation="SLNSEAAAASSAHVTAQQNWLRNTIKNNPAEFIIAFTHRPFYTG
                     DGKNAGQARDMRTWFQTIFDEYGVDLVLSGHNHVYARSFPILNGAKTATPGQGTVYIT
                     GTQIGDRYTSDPGTPMPEVDFAQAGKLDACNVITVTDQSLTVKLVNSNGDTLTTATFY
                     SKATLDSETFQSKMTVTSDPEDFSKATITFPNTNIARINKLEVIGKDEQLLASLVYPN
                     NQTTLQLTNIPKDAVEYPLNIKVLFRDGTTAQKEVILINEAKRFGTITNVRIEDNSDD
                     VSGLYWDSELVEGKIEKFELYIDDVLYASALPDENSILMPDVTPYEEHRLELRALYAD
                     KTVAFSEVLSFGEDTESVLLAYDFPSRTLKVGDKHTPTYVLVANETYPLEFKSSNEAV
                     AIVSDTGEITAIAVGNATITMNIKKWWSVNSSIEITVEAVEEPDPDPDPDPDPDHCGR
                     AADRLSC"
BASE COUNT          360 a          283 c          334 g          395 t
ORIGIN      
        1 gcaagataac cgatcggccg cgcggccgca atgatcgggg tcggggtcag gatccgggtc
       61 ggggtccggt tcttcaaccg cttcgacggt aatttcaatt gaagaattga cactccacca
      121 ttttttaata ttcattgtga tggtggcatt accaacagca atcgccgtaa tctcgcccgt
      181 atcggaaaca atagctaccg cttcattact tgatttaaat tctagagggt aagtttcatt
      241 ggcaactaag acatatgttg gggtatgttt atcgccgact ttcagcgttc ttgatggaaa
      301 atcgtaagct agtaggacgg actcggtatc ttcaccaaaa cttagtactt cggaaaaggc
      361 aacagtttta tcggcataaa gagcccttag ttctaaacga tgctcttcat aaggagtaac
      421 atccggcatt aaaatactgt tttcatcagg caaagcactc gcataaagaa catcatcgat
      481 atagagttcg aatttctcaa tctttccttc cacaagctca ctatcccaat aaaggccgga
      541 aacatcatca ctgttgtctt caatgcggac attagtaatc gtcccgaagc gtttggcttc
      601 gttaattaag atgacttctt tttgggcggt agtgccgtcg cggaacaaaa ctttaatatt
      661 taggggatat tcaaccgcat ctttgggaat attagtaagc tggagtgttg tttggttatt
      721 aggataaact aatgacgcca ataattgctc atctttgccg attacttcca gcttattgat
      781 gcgggcaatg ttggtattag ggaaggtaat cgtagctttt gaaaagtcct cagggtcact
      841 ggtaacagtc attttggatt gaaaggtttc ggaatcaagg gtcgctttgg aataaaaagt
      901 ggccgttgtc agggtatcgc cgttactgtt aactaattta acggttaaac tttgatcggt
      961 gacggtaatg acattgcaag cgtccagttt tcctgcttgt gcaaaatcaa cttccggcat
     1021 cggtgttccg gggtcgctag tataacggtc accgatttgc gttccggtaa tataaacggt
     1081 tccctgtccg ggggtggctg ttttcgcgcc gtttaaaatc gggaagctgc gggcataaac
     1141 atggttgtgg ccggataaaa ctaaatcgac accatattca tcgaaaatcg tttggaacca
     1201 tgtgcgcata tcgcgcgctt ggccggcgtt tttcccatca ccggtataaa agggtcgatg
     1261 agtgaaggca ataataaact cagcgggatt atttttgatg gtattgcgta accagttttg
     1321 ctgggccgtt acatgggcgg aagaagcggc ggccgcttcc gaattaagcg aa
//
LOCUS       DAOSGN010000295         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_100926_length_1372_cov_2.337889, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000295 DAOSGN010000000
VERSION     DAOSGN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100926_length_1372_cov_2.337889"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..>1372)
                     /locus_tag="PK087_04245"
     CDS             complement(97..>1372)
                     /locus_tag="PK087_04245"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOH18515.1"
                     /translation="DFETMNQSFADQALRVLAVGYQVWDSENQVIGLKHDELEHNLTL
                     LGLFGMIDPARPEVKDAIALTKQAGIITIMITGDHKNTAVAIAKELGILQKEHLTLTG
                     KELDAMTDEEFEQKLPQIRVYARVSPENKVRIVTAWKKTGLIVAMTGDGVNDAPSIKR
                     ADIGIAMGISGTEVAKGAADMILTDDNFATIVGAVSEGRAIFANIKKAIHFLLSCNIG
                     EIITIFLGVTIGLILFPNEIQLQILTAAQLLWVNLVTDSFMAIGLGLEPKEPDIMNYP
                     PRDNSKSIFSGGLGLKIAWQGIMIGLLTFLAFIIGYYMPGGGLRHAQTLSFMVLAISQ
                     LFHAFNVRSEHFSTFSLAKNNFLNMAFIGCLLLQLATVLVPTIANKVFGIDDVWHWRF
                     VDWLIVLALSVTPLVIVEMVKLVNKFSKKKVA"
BASE COUNT          420 a          294 c          249 g          409 t
ORIGIN      
        1 atgccaattg gcttaggagc attttttaaa aaagtctatc ttaaatttaa ataactatcc
       61 aagctagact ctttatatta ttacttaatt aatgttttaa gcaactttct ttttggaaaa
      121 cttattaaca agtttaacca tttctacaat aacaagcgga gtaactgata aagccaaaac
      181 gattaaccaa tcaacaaatc gccaatgcca cacatcatca attccaaaca ccttattagc
      241 tatagttgga actaatacgg tcgctaattg tagcaacaaa cagccaatga aagccatgtt
      301 taagaaattg ttttttgcca gtgagaaagt tgaaaagtgt tcggagcgaa cattaaaagc
      361 atgaaataat tgggatattg ctagcaccat aaagctcaag gtttgagcat gtcttaaacc
      421 gccaccgggc atatagtaac caattataaa ggctaagaaa gttaataaac caatcataat
      481 tccttgccaa gcaatcttaa gtcctaaacc accactaaag atactcttgg aattatctcg
      541 gggtggataa ttcataatat ccggttcttt cggttccaac cctaaaccga tggccataaa
      601 cgagtcggtt acgagattaa cccaaagcag ttgcgcggct gtaagaattt gtaattgaat
      661 ttcattaggg aataaaatta aaccaattgt tacacctaag aaaatggtaa tgatttcacc
      721 aatattgcat gataagagga agtgaatggc tttcttgata ttagcaaaaa tggctcgtcc
      781 ttccgatacc gccccaacga ttgtggcaaa attatcgtcg gttaagatca tatccgccgc
      841 ccctttcgca acttccgtac cgcttattcc catagcaatc ccgatgtccg cccgtttaat
      901 ggagggggca tcattaactc catccccggt catcgcgaca attaaaccag ttttcttcca
      961 ggcggtgaca attcgcactt tattttccgg tgatacccgc gcatagacgc gaatttgtgg
     1021 taatttttgt tcaaattctt cgtcagtcat cgcatctagt tctttaccgg ttaatgttaa
     1081 atgctctttt tggagaatac ccagttcttt cgcaatcgcc acggcggtat tcttatggtc
     1141 gccggtaatc ataatagtaa ttattccggc ttgtttggtt aatgcaatgg cgtctttaac
     1201 ttccggtcgg gcagggtcaa tcattccaaa gaggccgagc aaagttaaat tgtgctctaa
     1261 ttcatcgtgt ttcaaaccga taacttggtt ttctgaatcc catacttgat atccgacggc
     1321 cagcacccgc agggcttggt ccgcaaagct ttgattcata gtttcaaaat ct
//
LOCUS       DAOSGN010000296         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_101569_length_1366_cov_3.197559, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000296 DAOSGN010000000
VERSION     DAOSGN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101569_length_1366_cov_3.197559"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /gene="hflX"
                     /locus_tag="PK087_04250"
     CDS             <1..936
                     /gene="hflX"
                     /locus_tag="PK087_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOH18516.1"
                     /translation="RRAKTKEALLQVEIATLRYRLPQLIGLRSYMARQRGTGSLSHGR
                     GIGETKLELDKRMIYKRIATAKKELNALTSLRKQQRQKRQKQQMKIVALVGYTNSGKS
                     STLNALLKQSVAIKKEVTEKDMLFTTLETATRLIKTKHNLQFLVTDTVGFISKLPHQL
                     IEAFKSTLEEITEADLIVHVVDGSNPQFEKQIEVTNTVLSEIGVSNIPMIYAFNKSDL
                     HSDYFYVPFKYEKALRISAIKNTNIDCLIALIEEQLFKTNLIVDYKIPFNEGQMMNYL
                     HENAIILETKQNENDYFIKAKVSPLVLEKMKPYEK"
     gene            complement(949..>1366)
                     /locus_tag="PK087_04255"
     CDS             complement(949..>1366)
                     /locus_tag="PK087_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18517.1"
                     /translation="SIMFPKLAYLKQRTTSKVYYQEYLYVLLRTEEQIFLFKKAKHLG
                     GFVYKLKKTDYHDDVLKTLCTKWGVKTSIMNQGKMIINQEKTIYHAYLLFVSKPMESA
                     NWLGYSIKDPSQWPNDPFEREMMLRLNLQEPFKIEK"
BASE COUNT          452 a          282 c          233 g          399 t
ORIGIN      
        1 cgacgggcca aaaccaaaga agctctgctt caagtggaaa ttgccacctt acgttaccgc
       61 ctgccccaat taatcggact gcgcagttat atggcccgcc aacgcggaac cggaagttta
      121 tcccacggtc ggggcatcgg cgaaaccaaa ctcgaactgg acaaacgcat gatttataag
      181 cgcattgcca ccgccaaaaa agaattaaac gccttaacca gtttacgcaa acaacaacgg
      241 caaaaacgcc aaaaacaaca aatgaagatt gtcgctttgg tcggttacac caactccgga
      301 aagtcatcaa ccttaaacgc ccttttaaaa cagtcggtgg cgattaaaaa agaagttacc
      361 gaaaaagata tgctctttac aaccctcgaa acagcgacgc gtttaattaa aaccaagcac
      421 aacctccaat ttttagttac cgataccgtt ggctttatta gtaaactacc ccatcaatta
      481 attgaagctt ttaaatcaac ccttgaagaa attactgaag cggatttgat tgttcatgtt
      541 gtcgatggtt ccaacccgca gtttgaaaaa caaattgaag taacgaatac tgtcttaagc
      601 gaaatcggcg ttagtaatat tcccatgatt tatgccttca ataaaagtga tttacatagc
      661 gattacttct atgtgccgtt taaatatgaa aaggctttgc gaatcagcgc catcaaaaac
      721 actaatattg attgtttaat cgccttgatt gaagaacaat tgtttaaaac caatctcatt
      781 gttgactaca aaatcccttt taacgaaggc caaatgatga actatttaca tgaaaacgcc
      841 attattttgg aaactaagca aaatgaaaac gactacttca ttaaagctaa agtttctccc
      901 ttagtcttag aaaagatgaa accatatgaa aagtgacccc gaatgaggtc acttttcaat
      961 tttaaacggt tcttgtaaat taagtcttag catcatttcc cgttcgaaag gatcattagg
     1021 ccattggctc gggtctttta tcgaatatcc cagccaattg gcactttcca ttggtttaga
     1081 aacaaacaat aaataagcgt gataaatcgt tttttcctga ttaataatca ttttcccttg
     1141 gttcataatt gatgttttta ccccccattt tgtacaaaga gtttttaaaa catcatcatg
     1201 gtaatcggtt ttctttaatt tgtaaacaaa gccgcccaag tgtttggctt tcttaaagag
     1261 gaagatttgt tcctcagttc gtaataacac atataaatat tcttgatagt aaactttact
     1321 cgttgttcgt tgttttagat aggctaattt agggaacatg attgat
//
LOCUS       DAOSGN010000297         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_101818_length_1364_cov_2.399542, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000297 DAOSGN010000000
VERSION     DAOSGN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101818_length_1364_cov_2.399542"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..697)
                     /locus_tag="PK087_04260"
     CDS             complement(62..697)
                     /locus_tag="PK087_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18518.1"
                     /translation="MQNDVVELVFILDRSGSMAGLEKQTIDGFNEMIAKQKSLPGEAV
                     VSTVLFDHEIILLHNRVNLQMIEPLSEEHYVVRGMTALYDAIGKSIIRTERVYRELYD
                     NEKPHKTLFVITTDGMENASTEFRLPEIKRMIEKHERLNRWEFLFLGANIDAIKTAES
                     FGIPSKNAANFRADAQGIKHKYVVLHDAIACFRKDVKSLGKDKTDDYFSSK"
     gene            complement(778..>1364)
                     /locus_tag="PK087_04265"
     CDS             complement(778..>1364)
                     /locus_tag="PK087_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNase III inhibitor"
                     /protein_id="HOH18519.1"
                     /translation="LKDALKATKQFLENHELTIFIVADKAVRASITVPYLRSIKNYLQ
                     VCYPSQEIFSSKMSVIIDDDFLQSKTKRLLVDLEGQKGETFVQALFRMIDEKGLDDVF
                     VYKKANIDRKLFSKIKSDWDYQPSKNTAISFALALELNLDETTDLLQKAGYALSDSHL
                     FDLLIKYFIEHERYDLYEINEVLVYYHQKTLGTW"
BASE COUNT          405 a          279 c          232 g          448 t
ORIGIN      
        1 ggatgtggac accattttac gcctgcttcg ttctcttaaa cggaagattc ctttttttta
       61 gctatttaga tgaaaaataa tcatccgttt tgtctttccc caatgactta acatctttgc
      121 ggaaacaagc aatggcatcg tggaggacaa catatttgtg tttgattcct tgggcatcag
      181 cacggaaatt agcagcgttc ttgcttggga tgccaaaaga ttccgctgtt ttaatagcat
      241 cgatatttgc tcctaaaaat aagaattccc agcggttaag gcgttcatgt ttttcaatca
      301 tcctttttat ttcagggaga cgaaattccg tgctggcatt ttccatacca tccgtcgtaa
      361 tcacaaacaa agtcttatgc ggtttttcgt tgtcatacag ttcccgataa acccgttcag
      421 tgcggataat acttttacca atggcatcat acaaagcggt catcccgcgc acaacataat
      481 gttcctcgct taaaggttca atcatttgca gattaactcg gttgtgaagg aggatgattt
      541 catgatcaaa caacacggtg gataccaccg cttcccccgg caaactcttt tgtttcgcaa
      601 tcatttcatt aaaaccatca atggtttgtt tttccaaacc ggccattgag ccgctgcggt
      661 ctaaaataaa aaccaattcc acaacatcat tttgcattta tataacctcc ttatggttta
      721 caacttcatc ttaccataag aataattaaa aaaggtcgct tgccaagcga cattttttca
      781 ccatgttcct aaggtttttt gatgataata aaccaagact tcgttgattt catataaatc
      841 atatctttca tgttcaatga aatatttaat aagtaaatca aagagatggc tgtcagataa
      901 agcataaccg gctttttgta ataagtccgt tgtttcatcc aaattcagtt ccaaggctaa
      961 agcaaaacta atggccgtat ttttactcgg ttggtagtcc caatcactct tgattttgga
     1021 aaacagttta cggtcgatgt tggctttttt gtaaacaaaa acatcatcga gtcctttttc
     1081 gtcaatcatt cgaaacaaag cttgtacaaa agtttcgcct ttttgacctt ctaaatccac
     1141 taataagcgt tttgtcttgg attgaaggaa atcatcgtca atgataactg acatttttga
     1201 agagaatatt tcctggctag ggtagcaaac ctgaagataa tttttaatag atcttaaata
     1261 aggtacagta atgctcgccc taaccgcttt gtcagcaaca ataaaaattg ttaactcatg
     1321 attttctaaa aactgtttcg tggccttaag agcatctttt aagc
//
LOCUS       DAOSGN010000298         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_101828_length_1364_cov_2.182582, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000298 DAOSGN010000000
VERSION     DAOSGN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101828_length_1364_cov_2.182582"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PK087_04270"
     CDS             complement(<1..642)
                     /locus_tag="PK087_04270"
                     /inference="COORDINATES: protein motif:HMM:NF017689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HOH18520.1"
                     /translation="MFIKSKGTHISEHRGHKEMSLKNPYVEFLNPSYVYIPLVEQNTP
                     CDVNVKVGDLVKLGQIIANKTGTFCLPIHASVSGEVISINEKMWHASGKMVATVKIKN
                     DFTESKCEAIKPQAFQDFTQEQIVEAVFNNGVVGLGGSGFPTYFKLKVKQAMAMVIIN
                     AAECEPYITTDYALIKDHLDQLIRGMKYLMKAVSAPKGFIAIKENKKTLIQKIK"
     gene            complement(755..1333)
                     /gene="recO"
                     /locus_tag="PK087_04275"
     CDS             complement(755..1333)
                     /gene="recO"
                     /locus_tag="PK087_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOH18521.1"
                     /translation="MDKNPFSNFRSPSKRVSDFNYGEVVQNFSATKTNIDKMRMVIVI
                     LDMINQLANHIDDKQTLFSFCNQILDLINNSFHPLYLQIFRLKLLYLLGVGPIFSKCV
                     NCGTSIKQGTFDLNNGGMVCVHCQNQTLTFFDPDVIEVIKFLYLTKLVFLSPPVLNQI
                     PNYLLEISQFLDHYYEHFLGFNSRAEAILKKL"
BASE COUNT          457 a          234 c          221 g          452 t
ORIGIN      
        1 ttttatcttt tgaataaggg tctttttgtt ttctttaatc gcaataaagc ctttgggagc
       61 agaaacagcc ttcatcaaat atttcattcc ccgtattaat tgatctaaat gatctttgat
      121 taaagcataa tcggtcgtta tataaggctc acattcagcc gcgttaatga taaccatcgc
      181 cattgcttgc ttaactttca atttaaaata agtgggaaaa ccacttccac ctaaaccaac
      241 aacaccatta ttgaaaactg cttccacaat ttgttcttgg gtaaaatctt gaaaagcttg
      301 cggcttgatg gcttcgcact ttgattcggt aaaatcattt tttattttaa ccgttgccac
      361 cattttaccg gaagcgtgcc acattttttc attgatggaa ataacttcgc cactgactga
      421 agcatgaatc ggcaaacaaa atgttcctgt tttattggca attatttgtc caagcttgac
      481 taaatccccg actttaacat taacatcgca gggtgtattt tgttcaacaa gggggatata
      541 gacataacta ggatttaaaa actccacata ggggtttttt aaagacattt ctttatgtcc
      601 ccgatgttca gaaatatgcg ttcctttact cttaataaac acgatgacct cctctaattt
      661 actatttaaa ccttgtttat tataacatag gctcattaaa aaaggaaaca ctttttaagc
      721 gcttatagaa gaaaaaaagc gaacttcata tcgcttaaag tttcttaaga atagcttctg
      781 ctcgactgtt aaaacctaaa aaatgttcgt aataatgatc taaaaattga ctaatttcta
      841 ataaataatt aggaatttga ttaagaacgg gtggggatag aaacactaat ttcgttaagt
      901 ataaaaactt gataacttca attacatcag gatcaaaaaa tgttaaggtt tggttttgac
      961 aatgaacaca aaccatcccg ccattgttta aatcgaatgt tccttgctta atgctcgtgc
     1021 cgcaattgac acatttggag aaaatggggc caactcctaa taaatagagt aattttaaac
     1081 ggaatatttg caaataaaga ggatgaaagg aattattaat taaatcaaga atttgattgc
     1141 aaaaggaaaa caaagtttgc ttgtcatcaa tatgattagc caattggtta atcatatcaa
     1201 ggataactat gaccattctc attttatcaa tatttgtttt agtagcggaa aagttttgca
     1261 caacttcgcc atagttaaaa tcggaaaccc tttttgaagg cgatctgaag tttgaaaacg
     1321 gatttttgtc aattcattgc tataggcata gttatgactt ttgg
//
LOCUS       DAOSGN010000299         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_102466_length_1358_cov_4.016884, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000299 DAOSGN010000000
VERSION     DAOSGN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102466_length_1358_cov_4.016884"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PK087_04280"
     CDS             complement(<1..1340)
                     /locus_tag="PK087_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOH18522.1"
                     /translation="MDGIVYEGESSLDVSALTGESIHKDVRPGHPVLAGSINTNGTLK
                     VKVTSIYEDSMVAKILNLVENASSHKTKSETFISKFARYYTPTVVILAVVLAIVLPFI
                     LPTYDLSWTNGFKRSILLALIFLVISCPCALVLSVPLGFFGGIGGASRQGILIKGSNY
                     LEALNTVDTIVFDKTGTLTTGKFVVSDVLSFGKLSPEQILEYAAHCELNSNHLIAKSI
                     VEAYGKERLTLSRIASPKQMPGLGVSNFVDGLKIVVGKKSFLEQEGLSVPAIDNAATK
                     VYVAVGDKTEGCIIVKDQIKQNTHRAIADLKAMGIKRTVMLTGDTEEIAQEVATEVGI
                     DEVFAELTPLQKVEKLNDIKAQSDPHKKVAFVGDGINDAPVISSADIGIAMGGLGSDA
                     AIQVADIVLMTDDLSKLPIAKKIAKKTHNIVVQNITLALGVKFIVLILALIEPF"
BASE COUNT          393 a          308 c          272 g          385 t
ORIGIN      
        1 ataaacggtt caatcaaagc aaggattaaa acaataaatt taacaccgag ggctaaggta
       61 atgttttgga caacaatgtt gtgcgttttt ttcgcaatct tcttggcaat cggtaatttg
      121 gataaatcat ccgtcattaa cacaatatcc gctacttgaa tcgcggcatc ggaacctagc
      181 cctcccatgg caataccgat gtcggcacta ctgataaccg gggcatcgtt aatgccgtca
      241 ccgacaaaag cgactttttt gtgaggatcg ctttgggctt taatgtcgtt tagtttttcg
      301 accttttgca agggtgtcag ttccgcaaac acttcatcaa tcccaacctc agtggcaact
      361 tcttgcgcta tttcttcagt atctccggtc agcatcaccg ttcttttaat tcccatggct
      421 tttaaatcgg caatggctct gtgggtgttt tgctttattt ggtcctttac aataatgcaa
      481 ccttccgttt tatcacccac cgccacataa actttcgtgg cggcattgtc aatggcggga
      541 acgcttaatc cttcttgttc taaaaatgat ttcttcccta cgacgatttt taaaccgtcg
      601 acaaaattgg aaacacctaa gccgggcatt tgtttaggag aagcaatccg tgacaaagta
      661 aggcgctctt ttccataagc ctcaacaatt gatttcgcaa ttaaatgatt ggaattaagt
      721 tcacaatggg cggcatattc caaaatttgt tcgggtgata atttcccaaa acttaaaaca
      781 tcgctaacga caaatttccc cgttgtaagc gtccccgttt tatcaaaaac aatggtgtca
      841 accgtattta aagcttctaa atagttgctg cctttaatga gaatcccttg gcggctggcg
      901 cccccaattc ctccaaaaaa accaagcggc accgatagaa ccaaggcaca gggacaagag
      961 ataaccaaaa aaatgagggc caataaaatt gatcttttaa aaccgttcgt ccaactaagg
     1021 tcataagtcg gtaaaataaa gggtaaaaca atggctagaa caacggctag aatgaccact
     1081 gtcggagtgt aataacgagc aaatttggaa atgaaagttt ccgatttcgt tttgtggctg
     1141 ctcgcgtttt ccacaagatt caaaatcttg gcgaccattg agtcttcata aatgctcgta
     1201 actttaactt ttaaagtgcc gttggtgtta attgaacccg ccaaaaccgg atggccggga
     1261 cggacatctt tgtgaatgct ttcgccggtt aaggcggaaa catctaagga actttcacct
     1321 tcatagacaa ttccgtccaa aggaaggcgt tcacccgg
//
LOCUS       DAOSGN010000300         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_102601_length_1357_cov_3.428571, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000300 DAOSGN010000000
VERSION     DAOSGN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102601_length_1357_cov_3.428571"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1357)
                     /locus_tag="PK087_04285"
     CDS             complement(<1..>1357)
                     /locus_tag="PK087_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH18523.1"
                     /translation="GETTITAKLTAYPLITATISVRVFPFALTLTGSDEVFVGEEISL
                     TAIDRNKPDNIVVWESLNPNIATVDQNGKVTGHASGLATIRISSTVSKATLEKNIIVK
                     DRPFALTLTGSDEVFVGEEITLIGTDDNNPDNIVFWESLDPHIATVDQNGKVTGQASG
                     LVTIRISSFVSTETLEKTITVLVPAPVAIEVNAKTAGPYKSFGNVTLKHQVLPSGADQ
                     SVTWLSSNPEIATVDAQGKVSFLKIGEVEITAISQVNNSIIGAITMNIEVDPVALIKS
                     LNVTNPIVKYVTTYGNTEKQQWVYGSVSKYYPGELNLKENIIAITPTIDGQPNAYIGL
                     SYTSAIRAATEFKTVRSGVLKTAISDIIYHDTGNNNVGANADMHAKYIVGSANFNTYR
                     ARSWHYTVDDKEVIQHLPDNEVAWQGDTELAYSTTIGVETCVDFGSDLYTTWHRTAKL
                     MA"
BASE COUNT          375 a          317 c          261 g          404 t
ORIGIN      
        1 aagccatcag tttcgccgtc cgatgccaag tagtatataa atcactcccg aaatcaacac
       61 aagtttctac accaattgtt gtgctgtaag ccagttcggt atcaccctgc caagcaactt
      121 cgttgtcggg taaatgttga ataacttctt tatcatcaac cgtataatgc caagatcttg
      181 ccctataggt attaaagtta gcggacccga caatgtattt cgcatgcata tcggcattcg
      241 caccaacatt gttattaccg gtatcatgat aaatgatatc ggaaatcgct gtttttaaaa
      301 caccgcttct aaccgtctta aattcggtgg cagcccgaat cgccgatgta tagcttaagc
      361 cgatataagc attgggttga ccgtcaatgg tcggggtaat cgcaataatg ttttctttta
      421 aattcaattc tcccggataa tatttagaaa cactcccgta cacccattgt tgtttttccg
      481 tattgccgta agtcgttacg tatttcacaa tcggattagt gacatttaaa cttttaatta
      541 aagccaccgg atcaacttca atattcatcg tgattgctcc tataattgaa ttgttgactt
      601 gggagatagc cgtaatttcc acctccccga tttttaagaa actgacttta ccttgggcat
      661 caactgtggc tatttccgga ttactggata accaagtaac gctttgatcc gcaccgctgg
      721 gaagaacctg atgtttcaaa gtcacattcc cgaagctttt gtaaggtccc gccgttttcg
      781 cattcacttc aatggccacg ggcgccggaa caagcaccgt aattgttttt tccaaagttt
      841 ctgttgatac aaaggaggaa attcgaatcg tcaccaagcc actagcttgg ccggtaactt
      901 tcccgttttg atcaaccgtg gcaatatggg ggtccaagga ttcccaaaaa acgatgttat
      961 cggggttatt atcatccgtt ccaattaaag taatttcttc accaacaaag acttcatcgc
     1021 taccagtaag cgttaacgca aagggtcgat ctttaacaat gatgtttttt tctaaagtag
     1081 cttttgatac ggtcgatgaa atcctaatgg tcgccaaacc actggcatga ccggtaactt
     1141 tcccgttttg gtcaacggtc gcaatatttg ggtttaacga ttcccaaaca acaatgttgt
     1201 caggcttatt acgatcgatc gccgttaaac ttatttcttc accaacaaaa acttcatcgc
     1261 taccggtaag cgttaacgca aaggggaaaa ccctaacgct aattgtagcg gtaatcaagg
     1321 gataagccgt cagcttcgcc gtaatcgtcg tttcccc
//
LOCUS       DAOSGN010000301         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_102612_length_1357_cov_3.097542, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000301 DAOSGN010000000
VERSION     DAOSGN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102612_length_1357_cov_3.097542"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PK087_04290"
     CDS             <1..630
                     /locus_tag="PK087_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18524.1"
                     /translation="YYAPTLGTLTNAQITALIDLIVAGVPVEEIATEMEMTKAEAQAV
                     VDLGEGLIRKVLPNLHTLVKSLMAYVVTNDMITKIKTLEATIDSYEGEDFEEYDHNMT
                     AIFISTHLSAYLTNANQSLIRGIITDLATFAKNESIYPLLGATSLTDVTEMETMVNGT
                     FDQIVSLAGEIKDYKIATLTQAQKAKIEEFGSLVAFLFDGPDQDDGPVK"
     gene            954..>1357
                     /locus_tag="PK087_04295"
     CDS             954..>1357
                     /locus_tag="PK087_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18525.1"
                     /translation="MLKKFLFIFLLLFSVFGLAACNDDNKPKETESVDVPVNLVISDK
                     VLTWDAVAKATGYIVYVNDVEKATVTTTSYDFSALSEEKLVFQVVAKAPKGMTNSQKS
                     AALSYTTAAVLSAPANLAFKGKVLTWDAVKQAT"
BASE COUNT          422 a          227 c          274 g          434 t
ORIGIN      
        1 tattatgccc caactttagg aactttaaca aatgcgcaaa ttacggccct tattgatttg
       61 attgttgccg gtgttccggt agaagaaatt gcgaccgaaa tggaaatgac caaagccgaa
      121 gcacaagcgg tagttgatct tggggaagga ttaatccgta aagttcttcc taatcttcat
      181 actttagtta aaagcttaat ggcttatgtt gttacaaacg acatgattac caaaattaag
      241 acattggaag caacgattga tagttatgaa ggtgaagact ttgaagaata tgatcacaat
      301 atgacggcca tctttatttc gactcattta agtgcctact taacaaatgc taaccaaagc
      361 cttattcgtg gtattattac cgatttagcc acctttgcca agaacgaatc tatttatcct
      421 ttgttgggag ctacttccct tactgatgtt acggaaatgg aaacgatggt aaatggtact
      481 tttgatcaga ttgtttcttt agcgggcgaa attaaggatt acaagattgc aacattaact
      541 caagcccaaa aagccaagat tgaagagttt ggatcacttg tggcattttt gtttgatgga
      601 ccggatcaag atgatggacc ggttaaataa aacccctaga ccgaaaggcg actttcggtc
      661 ttttttttta caattatgaa aaccttttta aaaaaattat ctaaaataat ggtattgcta
      721 agttaagcta tggtataata agcaagacat aatcatgggt ttaaaaaaca atcaggtgtt
      781 aaactaaaga tggttttgtc cattgctttg ttttctggta atctttaatt tctaatcgct
      841 taaggttagg gatatcattg atgacatttt acttttatac aaattgatgt caattatttc
      901 cttaaagaaa gatgaattta gtaacgaaag caattaattt agaggaggga tttatgttga
      961 aaaaattttt gtttattttt cttttgcttt tctccgtttt tggtttagcg gcttgtaacg
     1021 atgacaataa accaaaggaa acggaaagtg ttgatgttcc cgttaatctg gtgatttccg
     1081 ataaagtgtt aacttgggat gcggttgcca aagccaccgg ctacatcgtt tatgtgaatg
     1141 atgtggaaaa agcaacagtc accaccacat cttatgattt ttccgcttta agtgaggaaa
     1201 agttagtttt ccaagtggtt gcgaaagctc cgaaagggat gactaactcc caaaaaagcg
     1261 ccgcccttag ctatacgacg gccgctgtct taagtgctcc cgctaacctt gcttttaagg
     1321 gtaaggtatt aacttgggat gcggttaaac aagccac
//
LOCUS       DAOSGN010000302         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_102862_length_1355_cov_2.879231, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000302 DAOSGN010000000
VERSION     DAOSGN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102862_length_1355_cov_2.879231"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK087_04300"
     CDS             <1..212
                     /locus_tag="PK087_04300"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOH18526.1"
                     /translation="AGYVVEIDYRNTSMKPQFRLAETKNARFIIIIGEEELQNNTYTI
                     KNTILKTQVSIAQSDIFKYLKENQK"
     gene            209..>1355
                     /gene="aspS"
                     /locus_tag="PK087_04305"
     CDS             209..>1355
                     /gene="aspS"
                     /locus_tag="PK087_04305"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOH18527.1"
                     /translation="MRTHHNNQLRMQNVGEVVTLVGWVQKKRHLGGLIFVDLRDREGI
                     TQIVCKPNNPNYAHLEKVRNEFVILVKGEVVERESKNFNLPTGEIEVEISELKVLSEA
                     EQPPMIIADETDALEDLRMKYRYLDLRRPIMQKNLMLRHRLTKTIREYFDNLGFVDVE
                     TPVFGKSTPEGARDYLVPSRVHPGKFYALPQSPQIYKQLLMIAGFEKYYQIVKCFRDE
                     DLRADRQMEFTQIDMEMSFVDEEDIYLLIDGMLAKVMKDIKNVNLPLPLPRLTFEEAM
                     SRFGTDKPDTRFAMELVDIHKAVNHLDFQVFQNTLKEQGLIKAINVKNGAQVYSRKEI
                     DRLTQEVKKYRAQGLMWLKYENQQFSGSIVKYLDEKAETQLIKLLNIQ"
BASE COUNT          476 a          243 c          256 g          380 t
ORIGIN      
        1 tagcgggata tgtcgttgaa atcgattatc gtaacaccag catgaaaccc caatttaggc
       61 ttgcagaaac gaaaaatgct cgtttcatca tcattattgg cgaagaagaa ttacaaaata
      121 atacctatac tattaagaac actatcttaa aaacacaagt ttccattgct caaagcgata
      181 tttttaaata tttaaaggag aatcaaaaat gagaacgcat cataacaatc aacttagaat
      241 gcaaaatgtt ggtgaagtag ttactttggt gggctgggtc caaaaaaagc gccacttagg
      301 cggtttaatc tttgtggact tacgagaccg tgaaggaatt acccaaattg tttgcaaacc
      361 taataaccct aattatgctc atttggaaaa agttcgcaat gaatttgtaa ttcttgtgaa
      421 aggagaagtt gttgagcgtg aaagtaaaaa ctttaattta ccaaccggag aaattgaagt
      481 agaaattagt gaattaaaag ttttaagtga agctgaacaa ccaccaatga ttattgctga
      541 tgaaactgat gctttggaag atttacgcat gaaatatcgc tacttggact taagacgacc
      601 gattatgcaa aaaaatctaa tgcttcgcca tcgcttaacc aaaacaatcc gtgaatattt
      661 tgataatctt ggttttgttg atgtggaaac gcctgttttt gggaagtcga ccccagaagg
      721 agcccgtgat tatttagtcc cttcccgcgt tcatcctggg aagttctatg cgcttccaca
      781 atcaccacaa atatacaagc aattactcat gattgccgga tttgaaaaat attaccaaat
      841 cgtaaaatgc ttccgtgacg aagatcttcg ggctgatcgc caaatggaat tcacacaaat
      901 tgacatggaa atgagttttg tggacgaaga agacatttat ttgctcattg acggaatgtt
      961 agcaaaagtc atgaaagaca tcaaaaatgt caatcttccg cttccgcttc cacgccttac
     1021 ttttgaagag gcaatgagcc gatttggaac agataaaccg gatacccgtt ttgctatgga
     1081 gttagttgat attcataaag cagtaaacca tcttgatttt caagtgttcc aaaacactct
     1141 caaagagcaa ggcttaatca aagccattaa tgtaaaaaac ggggctcagg tctattcacg
     1201 caaagaaatt gatcgtttaa cgcaagaagt taaaaaatac cgagctcaag gattgatgtg
     1261 gttaaaatac gaaaatcaac aattttcagg ttccatcgtc aaatatcttg atgaaaaagc
     1321 ggaaactcaa ttaattaaac ttcttaacat tcaag
//
LOCUS       DAOSGN010000303         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_102956_length_1354_cov_3.036952, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000303 DAOSGN010000000
VERSION     DAOSGN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102956_length_1354_cov_3.036952"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK087_04310"
     CDS             <1..240
                     /locus_tag="PK087_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOH18528.1"
                     /translation="MNMLGMGPAMIRSIMKKKNVDSLPTLMKNAMAMGVKVIACAMSL
                     DIMGIKPEELIDGVEIAGVATYLADTTEANHNLFI"
     gene            262..1269
                     /locus_tag="PK087_04315"
     CDS             262..1269
                     /locus_tag="PK087_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOH18529.1"
                     /translation="MINLCYTDDHQETKFINNTQKEFSEALILPQTWLYLSEPTEEEI
                     SLVAKTTKINEMLIRSLLDAEESAHIDEEGKVTVVVVDTPIVRMESKYPEVEHYTTIP
                     LANLINDEFVVTICTQKDIVIPNLIKNNKNLSPQKPVRLIIQILFRNAQIFVSKLKEV
                     DKGSEFIQTKLQEALKNSDLFELLNLGKSLVYLSTGLNSNQVVLEKLKNHPLFLQFQD
                     GLALIDDTIIENRQAMEMCAIHRDILNGLMDAYASVISNNVSSIMKTLTVLTIVLTIP
                     MLFASLFGMNFDLPLAAHNGFWITIVISLVLSLVLGYFLAIYTSKLRFRFSSKKKKTR
                     K"
BASE COUNT          435 a          235 c          256 g          428 t
ORIGIN      
        1 atgaatatgc tgggaatggg accggccatg attaggtcga ttatgaagaa aaagaatgtt
       61 gattctttgc caaccctaat gaaaaatgcc atggccatgg gggttaaggt tatcgcttgt
      121 gcgatgtcct tagatattat gggaatcaaa ccggaagaat taattgatgg cgttgaaatt
      181 gccggagtgg ctacttactt agccgatacc accgaagcta atcacaattt gttcatctag
      241 catgttgctg ggagaaataa aatgattaat ctatgttata ctgatgatca tcaggaaaca
      301 aaattcatca ataataccca aaaggagttt tcggaagcat tgattcttcc ccaaacttgg
      361 ttgtatttaa gcgaaccaac agaagaagaa attagcttgg ttgcgaaaac aactaagatt
      421 aatgaaatgt taattcgttc actgctcgat gcggaagaat ccgcacacat tgacgaagag
      481 ggcaaagtaa ccgttgtggt cgttgatacg ccgattgttc gcatggagtc caaatatccg
      541 gaagttgaac attatacaac tattccgctt gctaatctta tcaatgacga atttgtagtc
      601 accatttgta cacagaaaga tattgtcatt cctaatttaa tcaaaaacaa taaaaatctt
      661 tcgccacaaa aacccgttcg cttaatcatt caaattctct tccgcaatgc ccaaatcttt
      721 gtttccaaac ttaaagaagt ggacaaaggc agtgaattta tccaaaccaa acttcaagaa
      781 gctttaaaaa acagtgattt gtttgaattg ctgaacctag gaaaatcttt agtttaccta
      841 tcaaccggtc ttaattccaa ccaagtggtt ttggaaaagt taaaaaacca tccactgttt
      901 ttacaattcc aagacggatt ggcgttaatt gacgatacaa ttattgaaaa tcgccaagcc
      961 atggaaatgt gtgccatcca tcgggatatt ttaaatggtt tgatggatgc ttatgccagt
     1021 gtcatttcca ataatgtcag ttcgattatg aaaactttga ctgttttaac gattgtttta
     1081 accattccga tgctgtttgc cagtttgttt ggaatgaact ttgatttgcc tttagctgcc
     1141 cataatggtt tttggataac tattgtcatt tctttagtgt taagccttgt gcttggttat
     1201 tttttagcca tatatacgag taaattacgt tttcgcttca gcagtaaaaa gaaaaaaaca
     1261 cggaagtagt aaaagtcgcc acaagcgact tttattatta taatatttct ttaatcgact
     1321 tggatatgag cttaaatgtt gtttgttaat aata
//
LOCUS       DAOSGN010000304         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_102964_length_1354_cov_2.894534, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000304 DAOSGN010000000
VERSION     DAOSGN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102964_length_1354_cov_2.894534"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="PK087_04320"
     CDS             <1..123
                     /locus_tag="PK087_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011183057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18530.1"
                     /translation="VSKKTSLVVCGSDAGSKKTQAEKLNVKIINEAEFKALIGE"
     gene            125..>1354
                     /locus_tag="PK087_04325"
     CDS             125..>1354
                     /locus_tag="PK087_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOH18531.1"
                     /translation="MQKYIEIKGARENNLKNISLKIPKEKFVVMTGLSGSGKTSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNVNKPDVDSIEGLSPAIAIDQKSANKNPRSTVGTV
                     TEIYDYLRLLYARIGKSYCPEHHVEISAQTIPQMLDKIYEAAEESRIIIFSPAVFGQK
                     GTQEKTLDLIRKEGFSKVRVDGILYDLDEDIKLDKNKKHHLDIVVDRVKLKPEARSRL
                     FNSLETALKFGDGKVIVSVEGAETMYSEHLACPYCDFSIPKLEPRLFSFNSPFGACPE
                     CKGLGVLQKVDVNLLVTNPELSIRQGAIKYLRNIVDTENIEWQMFEKMSNHYGIDLNK
                     PYQDLSDEEKEIILVGSKEPITYQITTRSANTFRKTQYIEGIATLIERRYLETTSNFS
                     REYYNSYLADYECPECHG"
BASE COUNT          436 a          250 c          292 g          376 t
ORIGIN      
        1 gtgagtaaaa aaacctcctt ggttgtttgt ggtagcgatg ccggaagcaa gaaaacccaa
       61 gccgaaaaac tcaatgtcaa gattatcaat gaagcagaat tcaaagcatt aataggtgaa
      121 tagcatgcaa aaatatatcg aaatcaaagg tgctcgcgaa aacaatttaa aaaacatcag
      181 tttaaaaatt ccgaaggaga aattcgtcgt tatgacgggt ttatccggaa gtgggaaaac
      241 atctttggct ttcgatacga tttatgcgga agggcagaga cgctatgtgg agagcttatc
      301 cgcttatgcc cgccagtttt taggtaatgt taataaaccc gatgtcgatt ccattgaagg
      361 cttatcgccg gcgattgcca tcgaccaaaa gagtgccaac aaaaatcccc gttcaactgt
      421 gggaacggtt acggagattt atgattatct gcgtttgtta tatgccagaa tcggaaaaag
      481 ctattgtccg gaacatcatg tggaaataag tgcgcaaacg attcctcaaa tgctcgataa
      541 aatctatgaa gcggctgaag aatcccggat cattattttt agtccggccg tttttgggca
      601 aaaaggcact caggaaaaaa cccttgattt aatccgcaaa gaaggttttt cgaaagttcg
      661 ggttgatggc atcttgtatg atttggatga agacattaaa ctggataaaa ataaaaaaca
      721 tcatttagat atcgttgttg accgagttaa gttaaaaccc gaagctcgtt cccgtttatt
      781 taattcgctg gaaaccgctt taaaatttgg cgatggcaag gtcattgttt ctgttgaagg
      841 tgcggaaaca atgtatagtg aacatttggc ctgcccatat tgcgattttt ccattccgaa
      901 attagaacca cgcttgtttt cctttaattc gccctttggg gcttgcccgg aatgcaaggg
      961 tttaggagtc ttacaaaaag ttgatgttaa tttattagtc accaatccgg agttatcgat
     1021 ccgccaaggg gctattaaat acttacgcaa tatcgtcgat actgaaaata ttgaatggca
     1081 gatgtttgag aagatgagca atcattatgg cattgactta aacaaacctt accaagattt
     1141 aagcgatgaa gaaaaagaga ttattttagt cggttccaaa gaaccgatta cttaccaaat
     1201 aacgacaagg agcgccaaca ccttccgaaa aacccaatac attgaaggca ttgcgacctt
     1261 aattgaacgc cgttatttag aaaccacttc caatttttcg cgagagtatt ataattccta
     1321 tttagcggat tacgaatgtc cagagtgtca tggg
//
LOCUS       DAOSGN010000305         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_102984_length_1354_cov_2.471132, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000305 DAOSGN010000000
VERSION     DAOSGN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102984_length_1354_cov_2.471132"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..455
                     /gene="rplM"
                     /locus_tag="PK087_04330"
     CDS             33..455
                     /gene="rplM"
                     /locus_tag="PK087_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOH18532.1"
                     /translation="MANANTVEHKWFVVDATDLVLGRLSTEVATLLRGKGKPTYTPHV
                     DCGDYVIIVNAEKVALTGNKLDDKFYYRHSGYSGGLKSRSARQMLDIQPQKVLEKAIR
                     GMLPKTKLGDDMYRKLFVYVGPEHPHQAQKPEVYTLKG"
     gene            466..858
                     /gene="rpsI"
                     /locus_tag="PK087_04335"
     CDS             466..858
                     /gene="rpsI"
                     /locus_tag="PK087_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOH18533.1"
                     /translation="MSQVSYYGTGRRKSSVARVRLIPGSGVITINNRSFNDYVPSAAV
                     RLDVLQPLVLTNTSTSYDVVVNVNGGGISGQAGAIRHGITRALMEVNADFRPVLKRAG
                     LVTRDPRAKERKKYGLKKARRSPQYSKR"
     gene            991..>1354
                     /locus_tag="PK087_04340"
     CDS             991..>1354
                     /locus_tag="PK087_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH18534.1"
                     /translation="MDKMIKIYNSLTRTIEEFKPIHENQVMMYVCGPTVYDHIHIGNS
                     RPIIFFDVVARFFKYLGFEVTYVSNYTDIDDKIINRAKEEGLSEAEVSEKYIAEISNT
                     VRALNCLPHDANPKVTENM"
BASE COUNT          425 a          235 c          287 g          407 t
ORIGIN      
        1 tcatatacag gaggacaaaa tgagtaaatc ttatcgctaa tgccaacacc gttgaacata
       61 aatggttcgt cgttgacgca actgatttag ttctaggacg tttatcgacg gaagtggcaa
      121 ctttattacg cggaaaagga aaaccaacct atacgcccca tgttgattgt ggtgattatg
      181 tcatcattgt caatgctgaa aaagttgctt taaccggcaa taaactcgat gataaatttt
      241 actatcgcca ttcaggatat tctggtggtc ttaaaagtcg gagtgctaga caaatgttag
      301 acattcagcc gcaaaaagtt ttggaaaaag ccatccgcgg gatgcttcct aaaacaaaat
      361 taggcgatga catgtatcga aaacttttcg tttatgtagg gcctgaacat ccacatcaag
      421 ctcaaaaacc ggaagtttat accttgaaag gataggagtt aaaaaatgtc acaagtaagt
      481 tattatggaa cgggcagacg taaaagttcc gttgcccgcg taagattaat tcctggaagt
      541 ggtgtcatca ccattaataa tcgtagtttt aacgattatg tgccatcagc tgctgttcgt
      601 ttggatgtct tacaaccttt agttttaacc aatacttcca caagttatga tgttgttgtt
      661 aatgtcaatg gcggaggaat tagtgggcaa gccggagcca ttcgtcatgg cattacccga
      721 gcgttaatgg aagtcaatgc cgattttcgg cctgttttaa aacgagccgg tttggtaacg
      781 cgggatccgc gggccaaaga acgcaaaaaa tatggtttga agaaagcccg tcgttcacca
      841 caatattcaa aacgttaatc ggatctagag caagtcaatg ggcttgcttt tttcttagct
      901 tagttatgcg aaaatttgaa aaaaagcttt tgttctttac tttaaacatg gcaatgtata
      961 taataataat gcgtatttaa taatttggag atggataaaa tgattaaaat ttacaatagt
     1021 ttaacaagaa caatcgaaga atttaagcct attcatgaga atcaagttat gatgtatgtt
     1081 tgtggaccaa cggtctatga tcatattcat attggtaatt cgcgccccat tattttcttt
     1141 gatgtggttg ctcgtttttt taaatacctg ggctttgaag ttacctatgt ttccaattat
     1201 acggatattg atgataaaat cattaatcga gctaaagaag aagggctttc ggaagcggaa
     1261 gtttccgaga aatatattgc tgaaatcagc aatacggttc gtgctctcaa ttgtcttcca
     1321 catgatgcca atccgaaagt gacggagaac atgg
//
LOCUS       DAOSGN010000306         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_103469_length_1350_cov_1.800000, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000306 DAOSGN010000000
VERSION     DAOSGN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103469_length_1350_cov_1.800000"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1350
                     /gene="pulA"
                     /locus_tag="PK087_04345"
     CDS             <1..>1350
                     /gene="pulA"
                     /locus_tag="PK087_04345"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOH18535.1"
                     /translation="IDEGTTYQGITTGFDHIKELGVTHVQLLPFFDFGVVDETKLNEP
                     KYQAKGIFNWGYMPLNFNVPEGSYSSDPYDGTKRITELKQVTTAFTKNNIGLIMDVVY
                     NHTGLSADSNFHLILPGYFHRLTPTGVFSNGSGTGNETASERYMVRKFIVDSTVFWAT
                     EYNISGFRFDLMALHDVETMKAVVSALKAIDQNILIYGEPWNGGATPLPPSMAADKVN
                     LAKMPEVGAFNDEIRDGIRGSVFTTAEGGFVQGGTSPKIMNRTKYGVVGGVAYPGLDL
                     KDLSYQTVWHTQPSKTINYVSAHDNNTLYDKLRLSTTYQQKQYLDEMQKQANAIVLTS
                     QGVPFLHAGVEFLRSKPDGSGGYDHNSYESPDSVNQLRWDLKAEALNMSVFTYYKGLI
                     ALRKAHPAFRMATAKEVIANVKFVYEDKPGILAYTIANYANNDSWGTILVIHNNGNF"
BASE COUNT          384 a          319 c          289 g          358 t
ORIGIN      
        1 aattgacgaa ggcactactt atcaaggcat caccaccggc tttgaccata ttaaagaatt
       61 aggcgtaacc catgttcaac tactaccctt ctttgatttt ggggtcgttg atgaaacaaa
      121 actgaatgag ccgaaatacc aagctaaagg aatttttaac tggggctata tgccgcttaa
      181 cttcaatgtt cctgaaggtt cttattcttc cgatccttat gacggcacga aacgcatcac
      241 ggagttaaaa caagttacta cggcctttac caaaaataac atcggtttaa tcatggatgt
      301 ggtttataat cataccggtt taagcgccga ttccaatttt cacttaatcc ttccgggtta
      361 tttccaccgc ctaaccccaa cgggggtctt ttccaatggg tcaggaaccg gtaacgaaac
      421 cgcttcggaa cgctatatgg tgcgcaaatt tattgtcgat tccaccgttt tttgggccac
      481 ggaatataat atttccggat tccgttttga cttgatggct ttacacgatg tggaaaccat
      541 gaaggccgtt gtcagcgcct tgaaagcgat tgatcaaaac atcttaatct acggtgaacc
      601 gtggaatggg ggagcgaccc cgcttcctcc tagcatggca gcggataaag tcaatctcgc
      661 caagatgccc gaggtcggag ctttcaacga cgaaatccgc gacggcatta gagggtccgt
      721 ctttaccaca gcggaaggcg gttttgtaca agggggaact tcaccaaaaa tcatgaaccg
      781 caccaaatac ggtgttgttg gtggtgtggc ttacccaggg ctggatttaa aagatctttc
      841 ttaccaaact gtttggcata cccagccaag caaaaccatc aactatgtca gcgcccacga
      901 caataatact ttgtacgaca aattgcgttt atccaccacc tatcaacaaa aacaatatct
      961 tgacgaaatg caaaaacagg ccaacgccat tgtcctaact tcacaaggtg ttcccttcct
     1021 tcatgcggga gttgaatttc tacgttccaa accggatggc agcggcggtt atgatcacaa
     1081 ttcttatgag tcgccggaca gcgtcaacca actccgttgg gatttaaaag cggaagcgtt
     1141 aaatatgtca gtctttactt attacaaagg attgattgcc ttacgaaagg cccatccggc
     1201 cttccgaatg gcgacggcca aagaagtaat tgctaatgtg aaatttgttt atgaagacaa
     1261 accgggaatt ttggcctaca ccattgccaa ttatgccaat aacgattctt gggggacgat
     1321 tctcgtcatt cataacaacg gcaatttcat
//
LOCUS       DAOSGN010000307         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_103774_length_1347_cov_3.116873, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000307 DAOSGN010000000
VERSION     DAOSGN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103774_length_1347_cov_3.116873"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="secY"
                     /locus_tag="PK087_04350"
     CDS             complement(<1..875)
                     /gene="secY"
                     /locus_tag="PK087_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOH18536.1"
                     /translation="MALKNAKVIKMLLFTALILLIWRLAYHIQLPLINRDFLGTESSI
                     FGFLNVFSGGALANYSIVALGISPYITASIVIQLLQMDIVPALKEWAEEGETGKRKLN
                     QLTRYLALGLAFVQALTITLGIAAYYGDIFKLGVETNIFTYVYLALVMTAGTAFVLWL
                     AEKITLKGIGNGTSMFIVAGIVASFPRMISDLIKKFIAAPEGNSVGDIFIFFGVLLLM
                     IAIVVGVVFMEAAQRKIPIQYANRPAAAAFRGKSESNIPIKLNSASVIPVIFASTLMS
                     LPTTIVNFLDQSNNVV"
     gene            complement(876..1316)
                     /gene="rplO"
                     /locus_tag="PK087_04355"
     CDS             complement(876..1316)
                     /gene="rplO"
                     /locus_tag="PK087_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003216888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOH18537.1"
                     /translation="MKLHELKPVPGARRSKNIVGRGTSSGMGKTSGRGHKGQNSRSGG
                     GVRPAYEGGQTPLFKRFPKRGFTNHTRKEYEIVNLSQLNVFKEGTKVNHDKLFKQGLI
                     DKKESIVKILGNGTLTIKLTVIANKFSEAAKKGIVDAGGKIEVI"
BASE COUNT          416 a          294 c          247 g          390 t
ORIGIN      
        1 acgacattat tgctttgatc taagaagtta acaatcgtcg ttggtaagct cattaaggta
       61 cttgcaaaga tgacgggaat aaccgaagcg gaattaagct taatgggaat attcgattcc
      121 gatttaccgc ggaaagctgc cgccgccgga cgattagcat attgaatagg aatttttcgt
      181 tgcgcagctt ccataaaaac aaccccaaca acgatggcaa tcattaataa taagacccca
      241 aaaaagatga aaatatcacc aacggaattg ccttcaggag cagcaataaa ttttttaatt
      301 aagtccgaaa tcatccttgg gaaactggca acaatcccgg ccacgatgaa catcgaggtt
      361 ccgttgccaa tccctttcaa agtgattttt tccgcaagcc acaagacgaa agccgtcccg
      421 gctgtcatca ctaaagcgag ataaacatag gtgaagatat tagtttccac accgagtttg
      481 aaaatatcac cataataagc ggcaatgcct aaagtaatgg ttaaggcttg aacaaaagct
      541 aaacctaaag ctaagtatct cgtcagttgg ttcagttttc gttttccggt ttcaccttct
      601 tccgcccatt cttttaaagc cggaacgata tccatttgca ataattgaat gacgatggaa
      661 gcagtaatat agggcgaaat acctaaagca acaatcgagt aattggctag cgctcccccg
      721 ctaaagacat ttaagaaacc gaaaatcgag ctttctgttc ctaaaaaatc acggttaatt
      781 aacggtagtt ggatatgata agccaaacgc cagattaaga gaatcagggc ggtaaacaag
      841 agcattttaa ttacttttgc gttcttcaat gccaattaaa tcacctcgat ttttccgcca
      901 gcgtcaacaa tccctttttt agcagcttct gaaaatttat tagcaatgac ggttaatttt
      961 attgttaaag tcccatttcc taaaattttt acaatgcttt cttttttatc aattaatcct
     1021 tgtttaaata atttatcatg attaacttta gttccctctt taaacacatt taattgtgaa
     1081 agattgacaa tttcatattc cttacgggta tgattagtga aaccgcgttt cgggaaacgt
     1141 ttgaataagg gtgtttgtcc accttcataa gccggacgca caccaccgcc ggaacgagag
     1201 ttttgacctt tatgaccacg accgcttgtt tttcccatgc cgctggatgt ccctcgccct
     1261 acaatattct tgctacggcg cgccccaggt acgggtttta attcatgtag tttcatgtca
     1321 tcctcctatt gaacctcaac caaatga
//
LOCUS       DAOSGN010000308         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_103986_length_1345_cov_3.849612, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000308 DAOSGN010000000
VERSION     DAOSGN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103986_length_1345_cov_3.849612"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PK087_04360"
     CDS             <1..346
                     /locus_tag="PK087_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA (cytidine-2'-O-)-methyltransferase"
                     /protein_id="HOH18538.1"
                     /translation="VHRIKAFLNDAYTIFGNREVVIARELTKKYEEYIRGHLADVINR
                     EWELKGEFVVMIKGSKQALLAVELNKKTVEEHYQFYLDQNIDAKEAMKKVAKDRQMAK
                     SEIYRELLKPSK"
     gene            422..>1345
                     /locus_tag="PK087_04365"
     CDS             422..>1345
                     /locus_tag="PK087_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH18539.1"
                     /translation="MKVKHLNFNWLFCPYYQDEHLRDYANHEGFLVVDLPHSSQEYAY
                     NYLNEPACFGKYTYKYCLKKEELDLSTDVCYSLLFKGVAHQAEVYLNDYKLICHQGGY
                     DEFTVDLTKALKKDTDNWISVIVDESENPDIPPFGGMMDYLGYGGIYREVQLLTKNPD
                     CFQEVIIKSNEQMDGFVLEAEINGQADYIQGTVFFHKSPVETFQVELRNSATSFFGKI
                     NNLQKWDLQNPNLYDFHLQLIKDKNIVDETRIRFGFRKIKFTRRGFYLNDEPLKLRGL
                     NRHQSYPYVGYAMPKSAQVQDVKILKNQLGVN"
BASE COUNT          455 a          202 c          275 g          413 t
ORIGIN      
        1 cgtgcaccgc attaaggcct ttttaaacga tgcttatacc atcttcggca atcgggaagt
       61 tgtgattgcc agagaactga ccaagaaata tgaagagtat attcgtggcc atttagcgga
      121 tgttattaat cgggaatggg aattaaaagg cgagtttgtg gtgatgatta aaggaagcaa
      181 acaagcttta ctggcggtgg aacttaataa aaaaactgta gaagaacatt atcaatttta
      241 tctagatcaa aatattgatg cgaaggaagc catgaagaaa gtggcaaaag accgccaaat
      301 ggcaaaaagt gaaatttatc gagaactcct aaaaccatca aagtgatggt ttttttcaag
      361 aaagcatttt tgactttatt aaccattaat ttaatgctat aatatgaaaa ggtgatacat
      421 gttgaaagtt aaacacttaa actttaattg gctcttttgc ccttattatc aagatgagca
      481 tttgcgtgat tatgcgaacc atgaaggttt tttggtcgtt gatcttccgc atagcagcca
      541 agagtatgct tacaattatc ttaacgaacc agcttgtttt gggaaatata catataaata
      601 ttgtttaaaa aaagaagaac ttgatttatc tactgatgtt tgttattcgt tattatttaa
      661 aggggttgcc caccaagcag aagtttattt aaatgattac aagcttattt gtcatcaggg
      721 tggatatgat gaatttaccg ttgatcttac gaaagctctt aagaaggata ccgacaattg
      781 gattagcgtg attgttgatg aaagcgaaaa ccccgacatt cctccctttg ggggtatgat
      841 ggattatttg ggttatggcg gtatctatcg tgaagtgcaa ttgcttacta aaaatcctga
      901 ttgttttcaa gaagtcatca tcaaaagcaa tgagcaaatg gatggttttg tgttggaggc
      961 agaaattaat ggccaagccg attacattca aggaacggtt ttttttcaca aatctccggt
     1021 ggaaactttt caagttgaac taaggaactc agcaacaagc ttttttggaa aaattaacaa
     1081 tttacaaaaa tgggatttac aaaaccctaa tttatatgat tttcatttac agttaatcaa
     1141 agataaaaac attgttgatg aaacaaggat tcgtttcgga tttcgcaaaa tcaaatttac
     1201 aaggcgtggc ttttatttaa acgatgaacc gttgaaacta agaggcctta atcgccatca
     1261 aagttatccc tatgtcgggt atgcgatgcc gaaaagtgca caagttcagg atgtgaaaat
     1321 cttgaaaaat caattgggag ttaac
//
LOCUS       DAOSGN010000309         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_104269_length_1343_cov_2.633540, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000309 DAOSGN010000000
VERSION     DAOSGN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104269_length_1343_cov_2.633540"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..>1343)
                     /locus_tag="PK087_04370"
     CDS             complement(144..>1343)
                     /locus_tag="PK087_04370"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOH18540.1"
                     /translation="YELIPRDMTNDNVVNLNFLGAFEKRAIALGFVYNEERSLTTFSQ
                     ARAIVDDLMQEGLTSLSVAYTAWTNDEMEPAATASVKASGVLGRDRGFKSFNEYLEKN
                     NIALYPQVNITSNKGYDFLLGNLRYTAKSIGSTYASHRAYVLATGAPNSTIEPISMLS
                     PRFYESLARKYFNSYDRFHINGAFFSDLGNLRLGDYGAKKIIFAENGMDYQIATMDYL
                     AGKVDNMMLSAPFDYALKYTTFAVDVPLKSSMLGYYDYSIPFYQLVVSGLFDYTGPAV
                     NYDSERSMEWYLLKALETGSNLNFMISYEDTRNLLDTNYTMFYNAYYANWKSRILYMN
                     NVINDAKIHNNAILVGHKILQDNVYQVEYSNGAVLVINYNDTIYYDHVNGFSVRPNWF
                     TIVKEGM"
BASE COUNT          424 a          272 c          261 g          386 t
ORIGIN      
        1 agataaccaa aaaccttctt taaaaagagc attggtttgg tctgcataaa atcataacgc
       61 cattggctta gtttgtttaa aagacggccc caaaaggctt taatggaggc aaatgagcgt
      121 ttaaagacat tcgttttttc catttacatt ccctccttta caatcgtaaa ccagttcgga
      181 cgcacagaga agccgttgac atggtcataa taaatcgtgt cattatagtt gatgaccaaa
      241 acggcaccgt tgctgtattc gacttggtaa acattgtctt gtaagatttt atgaccgaca
      301 agaatcgcat tgttgtgaat tttagcatca ttgataacat tattcatata caaaattcga
      361 ctcttccagt tggcataata agcattgtaa aacatcgtat agttggtatc taataaatta
      421 cgggtatctt cataagaaat catgaagttt aaattggagc ccgtttctaa ggcttttaac
      481 aaataccatt ccattgagcg ttcgctgtcg tagttgacgg ccggtccggt gtaatcgaat
      541 aagccgctca caactaattg atagaaggga atcgaataat cgtaataccc gagcatagat
      601 gatttcaaag gcacatcaac cgcaaaagtg gtatatttta aagcataatc aaagggggcc
      661 gataacatca tattatcaac ttttcctgct aaataatcca tcgtggcaat ttgataatcc
      721 atgccgtttt ctgcaaaaat aatcttcttc gctccataat cacccaagcg tagattgcca
      781 agatcactaa agaaagcacc attaatatgg aaacgatcat aggaattaaa gtatttgcgg
      841 gctaaacttt cataaaagcg tggtgacaac atcgagattg gttcaattgt tgagttcgga
      901 gcaccggttg ctaaaacata agcacgatgg gaagcatagg tgctaccaat gcttttagcc
      961 gtatagcgta agttcccgag caagaaatca tagcctttgt tgctggtgat attgacttgc
     1021 ggatagagag caatattatt tttttctaaa tattcattaa aggacttaaa gccccgatcc
     1081 cgaccaagaa caccggacgc tttaacggag gccgtagccg ccggttccat ttcatcattt
     1141 gtccaagcgg tgtaagcgac gcttaaggac gtcaaaccct cctgcattaa atcatcaacg
     1201 atggctcttg cttggctaaa ggtcgttaaa ctgcgttcct cgttataaac aaagcctaaa
     1261 gcgatggccc gcttttcaaa agctcccaaa aagtttaaat taaccacatt gtcattggtc
     1321 atgtctcgag gaattagttc ata
//
LOCUS       DAOSGN010000310         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_105118_length_1336_cov_2.136612, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000310 DAOSGN010000000
VERSION     DAOSGN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105118_length_1336_cov_2.136612"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1186
                     /locus_tag="PK087_04375"
     CDS             272..1186
                     /locus_tag="PK087_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOH18541.1"
                     /translation="MFAFFKKRNVHVVMGMIIGTIIYCISVVWFLDLGEFYAGGVTGV
                     SQLISVSLSKLGINISKTIFISLLNIPLFVIGWKGVSKRFAVLSLASVLLQVVTIASL
                     EYFARQGFNPFLIIIQNPDASPKQGGMITLSILGGLLCGVGSGISLRAGASTGGMDII
                     SQYVSFKKNISFAKFSLLVDLGIIVVSDFVGSVEIAAYTVIRLIITVLVLDRIHTVYN
                     YLRVEIVTTEKEAMRLALISRFNHGITIFPAVGGFSNQEKWVLQTVVSSYEAEEYKNL
                     AMEIDSKSFITYTSVKQIYGFFNRNVIT"
BASE COUNT          354 a          208 c          275 g          499 t
ORIGIN      
        1 catggttttt tgagtgattt atcgggtgga gaatatattt taggagcttt tataatcgga
       61 acctttgtcc gctattccgg tttttctcgc aaaattgttt ccggaattag ccaaattttt
      121 tacagtgttt tttcccccat gtttttatct ttattttgag gaagcgatta tcttagcgag
      181 tatcttatta tgtgtgatag gaacgatttt tttaaaatca agtttccttt caatgaatac
      241 gccaaggaag aatgaggagg aagtaaggac gatgtttgcc ttctttaaaa aacgtaatgt
      301 tcatgttgtt atggggatga ttatcggaac cattatttat tgtataagcg ttgtttggtt
      361 tttggatttg ggagaatttt atgccggagg agtaaccggg gtttcgcaat taatttcggt
      421 ttctttaagt aaattgggga ttaacatcag taaaaccatt ttcattagtt tgcttaacat
      481 cccacttttc gtcatcgggt ggaagggtgt tagcaagcgt tttgccgtcc ttagcttagc
      541 atcggtatta ttacaagtgg ttacgattgc gtctttagaa tactttgctc gccaaggctt
      601 caatcccttt ttaattatta tccaaaaccc tgatgccagc cccaaacaag gagggatgat
      661 tactttatct attttaggtg gccttctttg tggtgttggt tcagggattt ctttgcgagc
      721 cggagcatca accgggggga tggatattat cagtcaatat gtatccttta agaaaaatat
      781 ttcgtttgct aaattttcat tattagttga tttaggaatt atcgttgttt ccgactttgt
      841 tgggagcgtt gaaattgccg cttacaccgt tattcgttta attattacgg ttttagttct
      901 tgatcgaatt catacagttt ataattatct gcgtgtggaa attgtgacga ctgaaaaaga
      961 agctatgcgg ctggctttaa ttagtcgctt taatcatgga ataactattt tccctgctgt
     1021 tggtggtttt tccaatcaag aaaaatgggt tttacaaacc gttgtttcca gttatgaggc
     1081 tgaagaatac aaaaatttag ccatggaaat tgacagtaaa tcgtttatta cctacacttc
     1141 ggtaaaacaa atctatggtt tttttaaccg caatgttatt acatgattac cgatttaggt
     1201 tccttagatt gaggggagga atttcttatt tttcttgact ttttttggtc aatgtagtat
     1261 tatagacaag gtacatttta aacccacaca aagccctgtg atttcaagaa tccacacttg
     1321 tttaatcata tacagg
//
LOCUS       DAOSGN010000311         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_105372_length_1334_cov_2.004691, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000311 DAOSGN010000000
VERSION     DAOSGN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105372_length_1334_cov_2.004691"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PK087_04380"
     CDS             <1..447
                     /locus_tag="PK087_04380"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOH18542.1"
                     /translation="ILVDIEIKEAGYEEEIVDLIHQYLSNDEFMIRSFEDQSVLAVKK
                     YDPQIITALLLGKDKPKHVVLTRLSELFPGFRLRRCRADYVSPHYRLLRFGFIKRMKW
                     LKKAVSVWTLNDENLIRKYLKMGVDSLVTDYPNLGLKLRDELSKKG"
     gene            complement(474..1208)
                     /locus_tag="PK087_04385"
                     /pseudo
     CDS             complement(474..1208)
                     /locus_tag="PK087_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
     gene            complement(1136..>1334)
                     /locus_tag="PK087_04390"
     CDS             complement(1136..>1334)
                     /locus_tag="PK087_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18543.1"
                     /translation="YLQDVMGFDRNRGLMKESEHPLHRVLGPAMFASPSTIMKTTSLQ
                     QSFPPSMKWVMPFMNNKLIRL"
BASE COUNT          398 a          224 c          284 g          428 t
ORIGIN      
        1 attctcgttg atattgaaat caaagaagcc ggttatgaag aggaaatcgt cgatttaatt
       61 catcagtatt tgagtaatga tgaatttatg attcgttctt ttgaagatca atccgtttta
      121 gccgtcaaaa aatatgatcc acaaattatt acggccttat tactggggaa agataaaccg
      181 aaacatgtgg ttttaacgcg gttgtcggag ttgtttcctg ggtttaggtt acgccggtgc
      241 cgtgctgact atgtttcccc ccattataga ttgttgcgtt ttgggtttat caagcggatg
      301 aaatggctta aaaaagcggt ttcggtttgg acgcttaatg atgaaaacct aatccgcaaa
      361 tatttaaaaa tgggtgtaga ttcattagtc actgattatc cgaatttagg tttgaaatta
      421 agagatgaat tatcaaaaaa gggttaacta ttttaaagct aaccctttta gtattaaaga
      481 ttgtagattt gtgaatattt ctcgattaag taattgatat aatattgggg ctggaatttc
      541 tccttaacga cattttcaaa taaggctttt ggatgttggg tagccccata gcggtggagt
      601 cgctctttaa accaagaatt gatggcttta gtagtacctt gttttagaga tttaaaaacg
      661 gggaaatctt ttttcatcgt tgctaacatt tgggcggcat aagccgaacc taaagcatag
      721 gaagggaaat aaccgaaccc gccaaacgcc caatgaacat cttgtaagac gccttctttg
      781 tcattaggaa catctaaatc taaatattct ttcatttttt cattccaaac ccgcggtaaa
      841 tcattaaccg taatttcgtt attgaaaagt gctttttcga tgtcatagcg tagcataata
      901 tggatagcat aggttaattc atcggcctca atgcgaatca atgaagcttg accacgattg
      961 atggactgat ggaaatcatt tagagtaata ccttttagtt gttttggaaa ggttttttta
     1021 agttttggaa aatgaacttt ccaaaattct ttactacggc cgatgatgtt ttcatagaag
     1081 cgggattggg attcatgcaa ggccatggaa gcaccaccgc cggaattggt ttgttctaaa
     1141 gccgaatcaa cttgttgttc ataaatggca tgacccattt catggatggc ggaaaagatt
     1201 gctgaagtga ggttgttttc ataatagtgg acggtgaagc gaacatcgct ggacccaaaa
     1261 cccgatgtaa cggatgctca ctttctttca tcaagccgcg gttgcgatca aaacccatca
     1321 catcttgtaa atag
//
LOCUS       DAOSGN010000312         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_105672_length_1331_cov_2.894984, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000312 DAOSGN010000000
VERSION     DAOSGN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105672_length_1331_cov_2.894984"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PK087_04395"
     CDS             <1..249
                     /locus_tag="PK087_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HOH18544.1"
                     /translation="PQVGIAKLEGTYLLWLDLRFLNMKGEDICHGLKALGMIVNEGQN
                     YGRECNGFIRINLACPQSYLEKGLLILEKFINSQTKTS"
     gene            477..779
                     /locus_tag="PK087_04400"
     CDS             477..779
                     /locus_tag="PK087_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18545.1"
                     /translation="MSIKNKVVAITPFICSIAFFLIGFLTGKWHPAWMVFLLVPLMPF
                     IVGEKKIRFSVPLVIAIIYVVASFITGLWHPLWVIFLSIPVFHIILTPTKKEPKDN"
     gene            927..1082
                     /locus_tag="PK087_04405"
     CDS             927..1082
                     /locus_tag="PK087_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HOH18546.1"
                     /translation="MKISKTLFIVLLLRVFLGKRFGNILKNKAILLGGIILLIIGLKI
                     LIEGLLK"
     gene            1091..>1331
                     /locus_tag="PK087_04410"
     CDS             1091..>1331
                     /locus_tag="PK087_04410"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOH18547.1"
                     /translation="MKIGFIGVGVMGSSMVKNLLKKAYAVEIYARHPEKVKEVVQAGA
                     TLIPSIATLAAQNELIITMVGFPKDVESIYFDDDKI"
BASE COUNT          411 a          192 c          271 g          457 t
ORIGIN      
        1 ccccaagtgg ggattgcgaa actggaggga acatatttat tgtggcttga tttacggttt
       61 ttaaatatga agggtgagga catttgtcat ggcttaaaag cactggggat gattgtcaat
      121 gaaggccaaa actacggacg agaatgcaat ggctttattc gaatcaattt agcttgccca
      181 cagtcttatt tagaaaaagg cttgttaatc ttagaaaaat ttattaattc acaaacgaag
      241 acgtcttaaa taattttctt tatcgttgat atttcttaaa aatggcgttt attttcattt
      301 agttgttttg caataattta taagaaaact taagaaaaaa cgagccagca gcaaatttag
      361 cgtgaattaa gcgctttatc gaagtaaggc ccgtttttat taaggaaaaa ggttttatat
      421 aaataatatt tgataaatga tgaaaaaaag agtataatcg aagaggagga atatttatgt
      481 cgataaaaaa caaagttgtt gccatcactc cttttatttg ttcaattgcc tttttcttaa
      541 ttggcttttt aacgggtaaa tggcaccccg catggatggt ttttttactg gtccccttaa
      601 tgccttttat tgttggtgaa aagaaaattc gtttttctgt tcccttagta attgccatta
      661 tttatgtggt agccagtttc atcaccggtc tttggcaccc gctatgggtt atttttctgt
      721 caattccggt ttttcatatt attttaacac cgactaaaaa agaacccaaa gacaattaaa
      781 atcatttttt taaagcaaaa aaagagatgt catctctttt ttcattagtg agtagatatg
      841 aattatggtg agattattat tctggagtgg gcttgttcat gagtgcggtg gcaatttctt
      901 tatgccatgg gctaatggaa acaaaaatga agataagcaa aaccctgttc atcgttttgt
      961 tattgagggt ttttctcggc aaacggtttg gaaatatctt aaaaaacaaa gccattttgc
     1021 ttggtggcat tattctttta atcatcggct taaagattct aattgaaggg cttttaaaat
     1081 aggagaacat atgaaaattg gttttattgg tgtcggggtt atgggtagtt ccatggttaa
     1141 aaatctgctt aaaaaagctt atgccgtgga aatctatgct cgtcatccgg aaaaagtaaa
     1201 ggaagttgtg caagccgggg cgaccttaat ccctagtatt gctactttgg cggcacaaaa
     1261 tgagcttatt atcacgatgg tcggctttcc taaggatgtg gaaagtatct attttgatga
     1321 tgataaaatc t
//
LOCUS       DAOSGN010000313         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_105820_length_1330_cov_2.432941, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000313 DAOSGN010000000
VERSION     DAOSGN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105820_length_1330_cov_2.432941"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PK087_04415"
     CDS             <1..561
                     /locus_tag="PK087_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOH18548.1"
                     /translation="VLIEKFQNIMVFAKDLRVKKLTMHLGYVNVELARDQYIKHILSV
                     LKKLCTFAASLGLALMIENMPSNSELGYAPEEIRTIIEQVGADNLGFIYDTGHAHVSE
                     FPDLSYLDVLGKYLWHCHLNDNHGQKDEHLAFGKGNFDFSSFFKKTINLGYQGLYCLE
                     ILFNDANDLRSYANSFDAIAKTLILQ"
     gene            621..>1330
                     /locus_tag="PK087_04420"
     CDS             621..>1330
                     /locus_tag="PK087_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH18549.1"
                     /translation="MVKIITDCTSDLSTEMYQQLDIEVIPLYVTIGGKTYQDGVDLTM
                     EELYQKIQQFGELPKSSAVSPAVMLEHFKKHYDKGDDVLFMGIGGQLSSTYQTARLAA
                     SEFPSNRVRVVDSQNLSTGIGLLLLKAAKFRDQGDDLETIVNKVEKIVPFVRTQFAIN
                     TLEYLHKGGRCSGTARIFGTWLKIKPIITVIEGQLYVAKKPRGKFTVALEVLLDYLRA
                     DKDRVDEDCLFITHSLAT"
BASE COUNT          404 a          211 c          278 g          437 t
ORIGIN      
        1 gttctcatcg aaaagtttca aaacatcatg gtttttgcaa aggatttgag agttaaaaaa
       61 ctgacgatgc atttaggata tgttaatgtt gagcttgctc gtgaccaata tataaaacat
      121 attttatcgg tattaaagaa actatgtaca tttgcagcgt ccttgggatt agctttaatg
      181 attgagaata tgcctagtaa tagtgaactt ggctatgctc cggaagaaat tcgaacgatt
      241 atcgaacaag ttggtgctga taatttgggc tttatttatg ataccggtca tgctcatgtt
      301 agtgaatttc cggatttaag ttatttagat gtcttaggaa aatatttgtg gcattgtcat
      361 ttaaatgaca atcatggaca aaaagatgaa catttagcct tcgggaaagg taattttgat
      421 ttttcttctt tctttaagaa gaccattaat cttggttatc aaggtcttta ctgtttagaa
      481 atcttgttta atgacgctaa tgacttgcgt tcatatgcca atagttttga tgccatcgca
      541 aaaactttaa ttcttcaata attaaagttg agaaaacaaa aagaatattg tataattatt
      601 ttgtatgtga ggtgaaagtg atggtaaaaa taattaccga ttgtactagt gatttaagca
      661 cagaaatgta tcaacaatta gacatcgaag tcattcctct ttatgtgact attgggggaa
      721 aaacttatca ggatggtgtc gatttaacta tggaagaact ttaccaaaaa attcagcaat
      781 ttggtgaatt gccgaagtca tccgctgtta gtccggccgt tatgcttgag cattttaaaa
      841 agcattatga caagggcgat gatgtcttat tcatgggcat tggtggtcaa ctttcttcaa
      901 cttatcaaac agcgcgatta gccgcttccg aattccccag caaccgtgta cgggttgtgg
      961 attcgcaaaa cctttccacc ggcattggtt tgttgttatt aaaagcggct aaattccgcg
     1021 atcaaggtga tgatttagaa acgattgtga ataaagttga aaaaatcgtt ccctttgttc
     1081 ggacccaatt tgcgattaac acccttgaat accttcacaa agggggtcga tgctcgggga
     1141 cagcccgtat ttttggaact tggttaaaaa ttaaacccat catcacggta attgaaggtc
     1201 aattgtatgt tgcgaaaaaa cctcgtggga agtttaccgt tgctttagaa gttttgctgg
     1261 attatttgcg ggcagacaaa gaccgtgttg atgaagattg tctctttatt acgcattcat
     1321 tggccacgga
//
LOCUS       DAOSGN010000314         1326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_106339_length_1326_cov_1.949646, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000314 DAOSGN010000000
VERSION     DAOSGN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106339_length_1326_cov_1.949646"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PK087_04425"
     CDS             complement(<1..925)
                     /locus_tag="PK087_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOH18550.1"
                     /translation="MTISFLDFINQLFANPILMVTVLLTLAVILINGWTDAPNAIATC
                     ISTKAISPRNAIIMATIFNFLGVFVMTLFNATVAQTIYKMVDFGGNAQDALAALCAGL
                     VGIVIWATLAWMFGIPTSESHALIAGISGAAIALQNGLAGINPNEWIKVLYGLLLSTV
                     LGFGSGWLMVKLIEGIFRKTDRNKTYVFFKNAQIAGGAAMAFMHGAQDGQKFMGVFML
                     GIFLAQGNPNAISFHIPIWLMILSSLVMALGTSIGGYRIIKTVGMDMVKLNQYEGFAA
                     DFSAATCLLISSIAGIPVSTTHMKTTAIMGVG"
     gene            1140..>1326
                     /locus_tag="PK087_04430"
     CDS             1140..>1326
                     /locus_tag="PK087_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18551.1"
                     /translation="MNQKKSFSIDEFFKRLFDKWVLKCPESQANSMSYRIALLNVNRL
                     QGIAVFLLFGVFFSLITD"
BASE COUNT          395 a          258 c          275 g          398 t
ORIGIN      
        1 tgccaacacc catgatggca gttgttttca tgtgggttgt cgaaacggga atcccggcga
       61 ttgatgatat caacaaacaa gtggcggctg agaagtccgc tgcgaaacct tcatattggt
      121 ttagtttaac catatccatg cccacggttt taataatgcg gtaaccacca attgatgttc
      181 ctaaggccat gactaaagaa cttagaatca ttaaccaaat cggaatatga aaactgatgg
      241 cattgggatt gccttgagcc aagaaaatgc ccagcatgaa gacccccata aatttttgcc
      301 cgtcttgggc accatgcata aaggccatgg cggctccacc ggcaatttgg gcatttttga
      361 aaaagacata ggttttattg cggtcggttt ttcgaaagat gccttcaatg agcttaacca
      421 ttaaccagcc actgccaaag ccaagaacgg tggacaaaag taaaccatag agaactttaa
      481 tccattcgtt gggattaatg ccggccaaac cgttttgtaa agcaatggcg gccccggaaa
      541 tcccggcaat taaagcatga ctttcgctgg tgggaatccc aaacatccaa gctaaagtgg
      601 cccaaataac aatccctacc aagcccgcac aaagggcagc tagggcatct tgggcattac
      661 cgccgaaatc gaccattttg taaattgttt gggcgacggt tgcattaaat aaagtcatga
      721 caaagacgcc aaggaagtta aaaattgttg ccatgatgat cgcatttctc ggtgaaatgg
      781 cttttgtaga gatgcaagtg gcaatcgcat tcggggcatc cgtccacccg ttaatcaaga
      841 tgacagctaa agttaagagg acggtcacca ttaaaatggg gtttgcgaaa agctgattga
      901 tgaaatctag aaagctaatt gtcataattg ctcctattta tatatttacc cttgtttatt
      961 ataccatttt tttatgatgg aacaaagatg attttccata ataaaacctt ttcataactt
     1021 tattaaagat gaaaatgctt tgatagttat tgatttcttc tctttttaca aattctttat
     1081 ggaattgttg gtaaatttat gttacaattt tagtaattat tattgatgtg aggttggtca
     1141 tgaaccaaaa aaagtctttt tccatcgatg agttttttaa acgtttattt gataaatggg
     1201 ttttaaaatg cccggaaagt caagctaata gcatgtccta ccgcattgcc cttctcaatg
     1261 tcaatcgctt acaagggatt gctgtttttt tgttgtttgg cgtgtttttt tccttgatta
     1321 ctgacg
//
LOCUS       DAOSGN010000315         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_106422_length_1325_cov_2.785827, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000315 DAOSGN010000000
VERSION     DAOSGN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106422_length_1325_cov_2.785827"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..436
                     /locus_tag="PK087_04435"
     CDS             17..436
                     /locus_tag="PK087_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOH18552.1"
                     /translation="MNELLVEVFNNILSIEEDVLKSRGIKLSMKEVHILETVRNTPVP
                     TMSAVARKLRITTGTLTASIDRLVRKKYVRRGQEEKDRRKVIIELTEPAFAVLKIHDE
                     FHNEMIEAIFADMKIDEDEILLKSLENISNYFKEKYR"
     gene            615..>1325
                     /locus_tag="PK087_04440"
     CDS             615..>1325
                     /locus_tag="PK087_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOH18553.1"
                     /translation="MDIFKKCEQVAHIEAVKARGVYPYFNPLETGQDTEVIINGRKTL
                     MIGSNNYLGLTSHPEVIQAGIDAIKKYGSGCSGSRFLNGTLDIHVALEKELSDFLHRE
                     ASLTFSTGFQSNLGIISALAGRNDVIVGDKENHASIYDACRLSYAKLLRNEHNDMDDL
                     EKILKSIGPEKGILIVTDGVFSMTGDICPLPEIVRLAKKYGARVMVDDAHGLGVLGKY
                     GRGTAEHFGLEEEVDVVMG"
BASE COUNT          424 a          211 c          290 g          400 t
ORIGIN      
        1 caagtcaaaa gctttaatca atgaattact cgttgaagtt tttaacaaca ttctaagcat
       61 tgaagaagat gttttgaaaa gtcgagggat taaactttcg atgaaagaag ttcatatttt
      121 agaaacggtg cggaatactc ccgtgccgac gatgtcggca gttgctagaa agttgcgcat
      181 taccaccgga accttaacgg cctccattga tcgattggtt cgcaagaaat atgtccgtcg
      241 aggtcaagaa gaaaaagatc gccggaaggt catcattgaa ttgacggaac cggcttttgc
      301 tgtgctcaaa attcacgatg aatttcataa tgaaatgatt gaagccattt ttgcggacat
      361 gaaaatagat gaagatgaaa tactcttaaa atctttggaa aatatttcta actattttaa
      421 agaaaagtac cgttaattgg ataataataa gcgataattt tgaagttaaa ttaaacatta
      481 aaaagctgat agtaccaatc agcctttcat atgttttccc atcaaattga tgttggaaca
      541 acagggcaag ttattaatta acttgaatct attcgcgatt gtgttataat aaaagaaatt
      601 atttggagga caatatggat atatttaaga agtgcgagca agttgctcat attgaagctg
      661 ttaaagcccg tggcgtttac ccctatttta accctcttga aacaggacag gataccgaag
      721 taataatcaa tggtcggaaa actttaatga ttggctccaa taattactta ggattaactt
      781 ctcatccgga agtgattcaa gcaggaattg atgccatcaa aaaatatggg tctggttgct
      841 cggggtcccg ctttttaaat ggaactttag atattcatgt tgctttagaa aaggaattat
      901 cggatttttt acatcgggaa gcatccttaa cttttagtac cggttttcaa agtaatttag
      961 gaattatcag tgctttagct ggccgcaatg atgtcattgt cggggataaa gaaaaccacg
     1021 cgagtattta tgatgcttgc cgcttaagtt atgctaaatt actgcgcaat gaacataatg
     1081 acatggatga tcttgaaaaa atcttaaaat ccattggtcc agagaaaggt attttaattg
     1141 taaccgatgg agttttctcc atgacaggtg atatttgccc tctgccggag attgtgagac
     1201 tggccaaaaa atatggcgct cgcgtcatgg ttgatgatgc gcatggttta ggcgtccttg
     1261 ggaaatatgg tcgtggtacg gcggaacatt tcggcttgga agaagaagtt gacgttgtta
     1321 tggga
//
LOCUS       DAOSGN010000316         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_106683_length_1323_cov_2.412461, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000316 DAOSGN010000000
VERSION     DAOSGN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106683_length_1323_cov_2.412461"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK087_04445"
     CDS             <1..430
                     /locus_tag="PK087_04445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOH18554.1"
                     /translation="GINTLKLVSEDIDNMGFAIPSNVVSEVVKDLEKGKQPFRRILGI
                     TILDVYAILNPEDFTNSDSFKNYTIPENITSGLYVESVANNQLASGYLFAGDIILAVD
                     EQKIYYVYDFRKLLNSIPVGQTLNLEVYRDNQIVKVNIPV"
     gene            441..>1323
                     /locus_tag="PK087_04450"
     CDS             441..>1323
                     /locus_tag="PK087_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH18555.1"
                     /translation="MNIFKKIFVFIFVLLGSSLLIGCDGSLFPNGNPIVVDDNQPTGV
                     TITVPPHGSVIEVGKTVQLTATVAPQTADQSITWTSNNEEVATIDSNGLVSGVGPGRV
                     KITATATATANSSKPKKAYAYFKVVLAPVEVTAITISGPIEVYVDGTIKLNYSLTPEN
                     ATTEVTWSVDNPDLATIDANGKLTGKAVGLVKVTATSKNKVTNTHQVNVLARTIPPEE
                     IIILGRNEIAIGKTVQLVVRTTPVGSINSVTWASSNPDVASVSETGLVHGISEGTTQI
                     SAVSTLNNSLQATFAIRI"
BASE COUNT          408 a          232 c          277 g          406 t
ORIGIN      
        1 tggtatcaac actttaaaat tagtatctga agatattgat aatatgggat ttgctattcc
       61 cagcaatgtt gtttcggaag ttgttaaaga tttagaaaaa gggaaacaac cgtttaggcg
      121 aatactagga atcacgattt tggatgttta tgccatttta aatccggaag attttactaa
      181 ttccgatagc tttaaaaatt atacaattcc tgaaaatatt acatcaggtt tatatgtgga
      241 aagtgttgct aacaaccaat tagcctccgg ttatttattt gccggtgata ttattttagc
      301 ggttgatgag caaaaaattt attatgtata tgattttcga aaactgttaa acagtattcc
      361 tgttggtcaa accttaaatt tagaagttta tcgcgacaat caaattgtca aggtcaacat
      421 tcctgtatga tggaggaaat atgaatatat ttaaaaaaat ttttgttttc attttcgttc
      481 ttttgggaag ttcactgtta atcggttgtg atggttcctt attccctaat ggcaatccga
      541 ttgttgtgga tgacaatcaa ccgactgggg taaccattac tgttccgcct catggctcag
      601 tcattgaagt tggaaagaca gtacaattaa cagcgaccgt tgctccgcaa actgctgatc
      661 aatcgattac ttggacaagc aacaatgaag aagttgcgac gatagatagt aatgggctgg
      721 tgagtggtgt tggtcctggc cgtgttaaaa ttacggcaac agccacagca acggctaatt
      781 catccaaacc gaaaaaggct tatgcttatt tcaaggttgt tttagctcct gttgaggtca
      841 ctgcgattac gattagtgga ccaattgaag tttatgttga tgggacaatc aaactgaatt
      901 attccttaac tccggaaaat gctacaacgg aagtcacctg gtcagtcgat aatcctgatt
      961 tggctacaat cgatgccaat ggcaagttaa ctggaaaagc ggttggcttg gttaaagtaa
     1021 ctgcaacaag caaaaataaa gttactaata cccaccaagt taatgttctt gcgagaacta
     1081 ttcctccaga agaaataatt attttgggta gaaacgaaat tgcaattggc aaaacggtgc
     1141 aattagttgt tagaactaca ccggtgggca gtatcaattc ggtgacatgg gccagctcca
     1201 accctgatgt tgcttctgtt tcagaaaccg gcttagtgca cggaatatcg gaaggaacaa
     1261 cgcaaatcag tgctgtttcg actttaaaca actcgcttca ggccactttc gctattcgga
     1321 tta
//
LOCUS       DAOSGN010000317         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_106830_length_1322_cov_2.164167, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000317 DAOSGN010000000
VERSION     DAOSGN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106830_length_1322_cov_2.164167"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PK087_04455"
     CDS             <1..655
                     /locus_tag="PK087_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH18556.1"
                     /translation="SLFYNGGGEEKMELLTYRTPRTKTGEKTFNKIIQAGKELFAQNG
                     FHATSVNLIIERAQIAIGTFYIYFDNKLMLYLYLLQQYKISIRQASREAIVGLTSRYD
                     IEKAGIKAFLSYVHKDPLAYKVIWESLFVDSNIFVDYYSDFSKSYIHQLEKHVPEEIN
                     KTIDLETLSFVLMGISNFVGLQVLFKKECTDEEIDRIADEVMKILSFGIFNKNANGV"
     gene            complement(695..>1322)
                     /locus_tag="PK087_04460"
     CDS             complement(695..>1322)
                     /locus_tag="PK087_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017473246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH18557.1"
                     /translation="VDFNAKKAIKLYLDEENTKLSDAAIRDYYQNLGITASEATDEQK
                     KAALELGQKAGVYYVDLMDTLVSSINTVINSISYVLIAFTAVSLVVSSIMIGIITYIS
                     VLERTKEIGILRSIGARKKDISRVFNAETLIIGFTAGVFGVGITLLLIIPINLILNHL
                     VDIPHLAALRVYHGLVLIIISMILTLVAGIIPSRIAAKRDPVEALRSE"
BASE COUNT          442 a          281 c          218 g          381 t
ORIGIN      
        1 ctcattgttt tataatgggg gcggtgagga aaaaatggaa ctattaacat atcgaacgcc
       61 acggaccaaa accggggaaa aaaccttcaa caaaatcatt caagccggca aagaactgtt
      121 tgctcaaaac ggtttccatg ctacatccgt caatttaatc attgaaagag cccaaatcgc
      181 gattggcact ttttatattt attttgataa taaattaatg ctctacctct atttattgca
      241 acaatataaa ataagcatcc gccaagcgtc ccgcgaagcc attgtgggct taacaagccg
      301 ctatgacatt gaaaaagccg gtatcaaagc ctttttaagc tatgttcata aggatcccct
      361 cgcttacaaa gtcatttggg aatctttatt tgttgattcc aatatttttg ttgattatta
      421 ttccgatttc tccaaatcct atattcatca actggaaaaa catgttcccg aggaaattaa
      481 caaaactatc gacttagaaa ccctttcctt tgtgctcatg ggcatttcca atttcgtcgg
      541 tttacaagtc ctgtttaaaa aagaatgtac agacgaagaa atcgaccgca ttgctgatga
      601 agtgatgaaa atcctatcct tcggtatttt taataaaaat gcgaatggag tttaacttta
      661 accgttaaac tccatttttt cttgaaaaca tcccttattc cgagcgtaag gcttcaaccg
      721 gatcgcgttt agcagcaatc cgcgacggaa taatgccggc aactaaagtt agaatcatcg
      781 aaatgataat taaaaccaaa ccatgataaa cccttaaagc cgccaaatgc ggaatgtcaa
      841 ctaaatgatt taaaatgagg ttgatgggaa taatcaaaag aagggtaatc cccaccccaa
      901 aaacaccggc cgtaaagcca ataatcaatg tttccgcatt gaaaacacgg gaaatatctt
      961 tttttctagc gccaatactg cgtaaaatcc caatttcctt cgttctttct aaaacggaaa
     1021 tataagtaat aatgccaatc ataatggaag acaccaccaa cgaaacagcc gtaaaagcaa
     1081 ttaacacata gctgatggaa ttaatcaccg tattgatgga actaacaagg gtatccatta
     1141 aatccacata ataaacaccg gctttttgac caagttctaa agccgctttc ttttgttcat
     1201 ccgtggcttc gctagccgta atccctaagt tttggtaata atccctaatg gccgcatcgc
     1261 tgagtttagt attttcttca tctaagtata acttaatagc ttttttcgca ttaaaatcaa
     1321 cc
//
LOCUS       DAOSGN010000318         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_106891_length_1321_cov_4.056872, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000318 DAOSGN010000000
VERSION     DAOSGN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106891_length_1321_cov_4.056872"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PK087_04465"
     CDS             <1..1111
                     /locus_tag="PK087_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOH18558.1"
                     /translation="RLLVKAAYAKKAANVIVRWGDDLINKMYYENVSEAVLQEVPHYL
                     VEQFKYFVDQKAAVITISAPTPGLLKDIDAKRLQLSAMATQKAIDFYRKHMMSNGSQW
                     CVIAYPTEAWAQKVFPGPKAFERLLDSILVASRVRDDNDPVAEWHEHMETLARHNAML
                     NQYNFLSLHFQNSLGTDLSVGLVENHLWGGGGEYTKDGIYFAPNIPTEETFTMPHKLK
                     VNGKVVATKPLNYQGKLIEDFWLVFKDGKVVDYDAKKEKEALKNLLEFDEGSSYLGEV
                     ALISFNSPISNMNVLFYNTLFDENASCHLALGNAYTMSIQGGNEMEESELLAKGYNKS
                     MTHVDFMFGSSDMKIIGITHEQKRITVFENGNFSF"
     gene            1170..>1321
                     /locus_tag="PK087_04470"
     CDS             1170..>1321
                     /locus_tag="PK087_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH18559.1"
                     /translation="MNQAKIYDIAGAAGVSLATVSRVLNHPEKVKESTRNKVLRIIRE
                     KGYKPNA"
BASE COUNT          408 a          234 c          301 g          378 t
ORIGIN      
        1 acgccttttg gttaaggctg cttatgcgaa aaaagccgct aatgttattg ttcgttgggg
       61 tgatgattta atcaataaga tgtattacga aaatgtttcg gaagccgttt tacaagaagt
      121 tccgcattac cttgttgagc aatttaaata tttcgttgac caaaaagccg ccgtcattac
      181 catcagtgcc cccacaccgg gcttacttaa agacattgat gctaagcgct tgcaattaag
      241 tgcgatggcg acgcaaaaag cgattgattt ttaccggaag catatgatgt ctaacggttc
      301 gcaatggtgc gtcattgctt atccgactga agcatgggcg caaaaagttt tccccggccc
      361 caaagctttc gagcgcttgc tcgacagtat tttagtggct tcaagagttc gtgacgataa
      421 cgatccggtt gcggaatggc acgagcatat ggagacatta gcccgccata atgcgatgtt
      481 aaaccaatat aattttttat ccctgcattt tcaaaatagt ttagggaccg atttgagcgt
      541 cggcttagta gaaaatcacc tttggggtgg gggcggtgag tatacaaaag acggtatcta
      601 ctttgctccg aatattccca ccgaagagac ttttacaatg ccgcacaaat tgaaagttaa
      661 cggaaaagtc gtggctacga aaccacttaa ttaccaggga aaattgattg aagatttttg
      721 gttggtcttt aaagacggga aagttgtcga ttacgatgcc aagaaagaaa aagaagcctt
      781 aaagaatttg ttggaatttg atgaaggcag ttcctactta ggtgaagttg cgcttatttc
      841 ctttaattca ccgatttcca atatgaatgt tttattttat aataccttgt ttgatgaaaa
      901 tgcttcctgc catttagctt taggcaatgc ctacaccatg agcattcaag gcggtaatga
      961 gatggaggaa agtgaactgt tagccaaggg ctataacaaa tcgatgactc atgttgattt
     1021 catgtttgga agtagcgaca tgaagattat cgggataacc catgagcaaa aacgaataac
     1081 tgtttttgaa aacggcaatt ttagctttta attttaggat tgaaaatatt ttcattgtat
     1141 gatataatca cataaaaaga ggtgagatga tgaaccaagc caagatttac gatattgccg
     1201 gagcagcggg tgtcagttta gcaactgttt cgcgggtttt aaaccacccg gagaaagtaa
     1261 aagaatcaac gagaaataag gtgttgcgca ttatcagaga aaaaggttat aaaccgaatg
     1321 c
//
LOCUS       DAOSGN010000319         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_107553_length_1316_cov_2.435369, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000319 DAOSGN010000000
VERSION     DAOSGN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107553_length_1316_cov_2.435369"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PK087_04475"
     CDS             <1..907
                     /locus_tag="PK087_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOH18560.1"
                     /translation="DAWAQKRLKLPLTVGHEFSGEIVKVGDKVTRVKIGDLVSAETHI
                     ICHECEFCLRGEGHICENTKVIGVDTDGCFARYVKIPEHNCFINSPTMNPLHLSVQEP
                     LGNAVHTIAHFDVNNKDVVILGCGPIGLMGVDVAKALGAKKVIAIEVKPYRAQLAQAI
                     GADVVINPLQEDVIARVFAETNGRGADVIGEFSGNKVAIEQAFKYLKAGGGMSMLGIP
                     SQKIELDFATEVVFKGISIYGVTGRRIYDTWYQVRDLLEKNLLHLDKIITHIFPLREI
                     NQAAEIMGSGNSGKIVLIPEDSIDE"
     gene            900..>1316
                     /locus_tag="PK087_04480"
     CDS             900..>1316
                     /locus_tag="PK087_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH18561.1"
                     /translation="MNNLQFLEDKIAELKEQGVYRQLPVNTGPCANVIYLKGQEIVNL
                     SSNNYLGFANHPRLIQAAKAATDKYGAGAGSVRTIVGNQDLLEELENLLALFKQEEAV
                     TCFQSGFNCNLGTIPAITEAGDLIVSDEKNHASIIDG"
BASE COUNT          373 a          235 c          326 g          382 t
ORIGIN      
        1 tgatgcttgg gcccaaaaac gtttaaaatt acctttaacg gttggccacg aattcagtgg
       61 cgagattgtg aaagtggggg ataaagtaac gagggtcaaa attggcgatt tggttagtgc
      121 cgaaacccat attatttgtc atgaatgtga gttttgtttg cggggggaag gccatatttg
      181 tgaaaacacg aaagttatcg gtgttgatac tgacggctgt tttgctcgtt atgtgaaaat
      241 tccggaacat aattgtttca ttaatagtcc cacgatgaat cctttgcatt tgtctgtgca
      301 agaacctttg ggaaatgcgg tccatacgat agctcatttt gatgtgaaca acaaagatgt
      361 tgtgatttta ggttgtgggc cgataggttt aatgggggtt gatgttgcga aagcccttgg
      421 cgctaaaaaa gtcatcgcta ttgaagtgaa accttatcga gcgcaattgg cccaagctat
      481 tggggccgat gtcgttatca atccgcttca agaagatgtc attgcccggg tttttgcgga
      541 aaccaatggt cggggagccg atgttatcgg tgaatttagc ggtaataaag tcgcaattga
      601 acaggccttt aaatatctta aagccggtgg ggggatgtcg atgttaggga ttccttccca
      661 aaaaattgaa ctggattttg cgaccgaggt ggtttttaaa ggcatcagca tttatggagt
      721 caccggtcgt cgcatctatg atacttggta tcaagtgcgt gatttattag agaaaaacct
      781 tttacattta gataaaatca ttacccacat cttcccctta cgggaaatta accaagctgc
      841 ggaaatcatg ggcagtggca acagtgggaa aatcgtctta attccggagg acagtattga
      901 tgaataattt acaattttta gaagataaaa ttgcggaact aaaggaacaa ggtgtctatc
      961 ggcaactacc ggtgaatacg ggtccctgtg ccaatgtcat ttacttaaag ggccaagaaa
     1021 ttgtcaattt atcaagtaat aattatctag gttttgccaa tcatccgcgg cttattcaag
     1081 ctgctaaagc ggctactgat aaatatggcg ctggcgccgg cagtgttcgc accattgttg
     1141 gcaatcaaga tttgttggaa gaactggaaa acctcttagc tttgtttaaa caagaagaag
     1201 cggtaacttg ctttcagtcc ggtttcaact gtaatttagg aacgattcct gccatcaccg
     1261 aggccggtga tttaatcgtc agtgatgaaa aaaaccatgc ctcgattatt gacggc
//
LOCUS       DAOSGN010000320         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_109137_length_1303_cov_3.274840, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000320 DAOSGN010000000
VERSION     DAOSGN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109137_length_1303_cov_3.274840"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="trmFO"
                     /locus_tag="PK087_04485"
     CDS             complement(<1..972)
                     /gene="trmFO"
                     /locus_tag="PK087_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOH18562.1"
                     /translation="MQAVNVIGAGLAGSEATYQLIKRGVKVNLWEMRPSKMTEAHQTA
                     LFSELICSNSFRAASIENAVGLLKEEMRRLDSLIMRSADANQVEAGGALAVDRTMFAE
                     MITKTIKQNPLVNIINQEAISIPEGPTIIASGPLTSLAFSEAIKAFVQEDYLYFYDAI
                     APIVYLDSVDKSRVYLKSRYDKGTAAYYNCPFTKEQFDYFYEELLHAKRVVPHEFEMK
                     IFEGCMAIEDMAARGKETLLFGPMKPVGLRNPFTNELPYAVVQLRQDDAAKTMYNLVG
                     FQTHLTFPEQKRILRLIPGLERCEIARYGVMHRNTFINAPQLLNCYYQ"
     gene            complement(1033..>1303)
                     /locus_tag="PK087_04490"
     CDS             complement(1033..>1303)
                     /locus_tag="PK087_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH18563.1"
                     /translation="LLYKDVYQDYKKAQYYYLEGISQDKNNSILWYNLACLYVVMNDF
                     ENAYNCFLYANLKDKSIFKYMENDEELDMFRQTETYQKLKQNLKG"
BASE COUNT          395 a          283 c          228 g          397 t
ORIGIN      
        1 ttgataataa caattaagca actgcggtgc attaataaag gtattgcgat gcatcacccc
       61 atagcgagca atctcgcaac gttctaaacc gggaataagc cgtaaaatcc gtttttgttc
      121 ggggaaggtc aaatgggttt gaaagccaac taaattgtac atcgttttag cagcatcatc
      181 ctgacgcaac tgcacaacgg catagggtaa ttcatttgta aaaggatttc gtaaaccgac
      241 cggtttcatc ggtccaaaca ataaggtttc tttcccacgg gcagccatat cctcaatcgc
      301 catgcaccct tcaaagattt tcatttcaaa ttcatgggga actacccgtt tggcatgaag
      361 caattcttca taaaaataat caaattgttc ttttgtaaac ggacagttgt aataagctgc
      421 cgtcccttta tcataacgag atttcaaata aacccgactt ttgtcaaccg aatcaagata
      481 aacaatcggg gcaatcgcat cataaaaata taaatagtct tcctgaacaa aagctttaat
      541 ggcttcactg aaagccaaac tagttaacgg accgctggca ataatggtgg gtccttcagg
      601 aatcgatatt gcttcttgat tgatgatatt aactaagggg ttttgtttga tggttttcgt
      661 aatcatttcc gcaaacatcg ttcgatcaac cgccaaggcc ccgcccgctt ccacttgatt
      721 ggcatcggca gagcgcataa tcaaagaatc caagcgccgc atttcttctt tcaagaggcc
      781 aacggcgttt tcgatgctcg ctgcccggaa ggaattggag caaattaatt cggagaacaa
      841 agccgtttgg tgggcttccg tcatcttact aggacgcatt tcccacaaat taactttcac
      901 acctcgtttt attaattgat aagtcgcttc actaccggct aaaccggctc ctataacatt
      961 gacagcttgc attttttcac ctgttttcgc ttattattat accatattcc caaccaaaac
     1021 aaaaaggctg attcagcctt ttaaattttg tttcagtttt tgataagttt ccgtttggcg
     1081 aaacatatcc aattcttcgt cattttccat atatttaaag atggatttat ccttgagatt
     1141 agcatataaa aaacaattat aagcattctc aaaatcattc attacaacat aaagacaagc
     1201 gaggttgtac cacaaaatgc tgttgttttt atcttggcta atcccttcta aataataata
     1261 ttgcgctttt ttataatcct ggtaaacatc tttgtaaagc aat
//
LOCUS       DAOSGN010000321         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_109290_length_1302_cov_3.039294, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000321 DAOSGN010000000
VERSION     DAOSGN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109290_length_1302_cov_3.039294"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /gene="buk"
                     /locus_tag="PK087_04495"
     CDS             complement(<1..1051)
                     /gene="buk"
                     /locus_tag="PK087_04495"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOH18564.1"
                     /translation="MMLEHLILVINPGSTSTKIAVFKNELSLMEIKISHPTEVIQSFP
                     SVASQFTFRKEMIIKSLQDKNFDLKTLSAVCGRGGMLAPMESGTYLINEKMLNFLKEA
                     KRGEHASNLGALIAHDIGLSYHIPAFIVDPVAVDEMEAIARISGMPLIERRCIFHALN
                     HKAIGRRVAQDHGRRYEDLNLIVAHLGGGISVGAHQKGRVIDVNDALDGDGPMSPERS
                     GSVPLGPLYRLCLSGKYTLEDIKRMNYGKGGLVAYLGTNDAKEVVKRIEQHDEHAKLI
                     YEAMIYQIAKEIGACATIFKGQFDFIVLTGGLAYEPYLVTHLIERIKFLGQVLVYPGE
                     NEILSLALGALRVLNQ"
BASE COUNT          389 a          300 c          228 g          385 t
ORIGIN      
        1 cttggtttaa aactcgcaaa gcccccaggg ccaatgacag tatttcattt tcccccgggt
       61 aaacgagaac ttgaccaaga aacttaatcc tttcgattaa atgcgtgact agatatggtt
      121 catatgctag tcctccggtc agaacaataa aatcaaattg gcctttaaaa atcgttgcgc
      181 aggcgccgat ttctttcgca atttggtaaa tcatggcctc ataaatgagt ttcgcatgtt
      241 catcatgctg ctcaatccgc ttaacaactt ctttcgcatc attcgttcct aaatatgcaa
      301 ctaaacctcc tttcccgtaa ttcattcgtt taatgtcttc aagagtatat ttccctgata
      361 aacataagcg atatagaggc cccaaaggca cggacccact gcgttccggt gacatcggtc
      421 catcaccgtc caaagcatca ttgacatcaa ttacgcgccc tttttgatgg gcccccacac
      481 tgatgccccc acctaaatga gcaacaatta aatttaagtc ttcgtagcgt cgcccatggt
      541 cctgggctac ccgccggccg atggccttat gatttaaagc atgaaaaatg cagcgccgtt
      601 caatcaaggg cattcccgaa atacgggcaa ttgcttccat ttcatcaacg gcaactggat
      661 caacgataaa agcgggaata tggtaggaaa gaccgatatc atgggcaatt aaggcgccca
      721 gattagaggc atgttcgcca cgcttggctt cttttaaaaa gttaagcatt ttttcgttga
      781 ttaagtaagt accgctttcc atcggtgcca acattccccc ccgaccacaa acagcactta
      841 aagtttttaa atcaaagttt ttatcttgta aactcttgat aatcatttct ttacgaaagg
      901 taaattggga agccacactt gggaaagatt gaatcacttc cgtcggatgg ctgattttga
      961 tttccataag actgagttca tttttaaata cggcaatttt tgtggaagtt gaaccagggt
     1021 taataactaa aattaagtgc tccaacatca tccctccttc ttggagtaaa aaagaaaccg
     1081 tttccataga tattatactt gaaacaaagc aatttctcaa tggctttata aagaaaattt
     1141 taaaacagct taagcttttt ccaactttcc ccgttcacaa aaacagtggt tgatttcaat
     1201 tgtgtcatca ttatgtggtt gcttttatcc tcatccccaa gtataataaa ccttggagac
     1261 aaaacgatga ttattaattt tttaaccttt aacattcaac ac
//
LOCUS       DAOSGN010000322         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_109839_length_1298_cov_2.903459, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000322 DAOSGN010000000
VERSION     DAOSGN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109839_length_1298_cov_2.903459"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK087_04500"
     CDS             <1..296
                     /locus_tag="PK087_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOH18565.1"
                     /translation="IEEEQLKVGIINARFIKPLDLETLKALSQTRVIIYEEVVLNGSL
                     ASLIIEANTLHALELKIQAIAIPNQFVSAGSVKSLKKDLGLDIRDILKTHLRG"
     gene            300..>1298
                     /locus_tag="PK087_04505"
     CDS             300..>1298
                     /locus_tag="PK087_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH18566.1"
                     /translation="MLISLNVKNFAIIDNMQIEFQDGMTCLTGETGAGKSLIIDAIGL
                     LFGKRASTDLIRFNETKATIEGVFTKLKPEMTEIIELNPDDDVLIIKREIYANGKSLC
                     KVNNQTVTLNQLNDLAALIGDIHTQYDTQGLFNPKNYLKFIDNSEVLGLIPSYQENLK
                     KYREFQNDYQKLLKRSIDDSQRLEFLKYQIKELEQAQLSLEEEGQLQQQANYLSNFEQ
                     ISTNINDVKEIFSEQQLLDHLYEALSLLKKVSQFDERYDIIQKKMEEIYYNLSDQIGE
                     INKLSKSLDFDPAELDRINERLGLYSELKRKYKKNLTDLLLFQKQISEEINLIENFD"
BASE COUNT          495 a          188 c          213 g          402 t
ORIGIN      
        1 ttattgaaga ggaacaacta aaggtcggga ttatcaatgc tcgatttatt aaacctttag
       61 atttagaaac gttaaaagca ttatcacaga caagagtaat catttatgaa gaagtcgttt
      121 taaacggctc tttagcgtca ttaataattg aagcgaatac tttacatgct ttagagctaa
      181 aaattcaggc gattgccatt cctaatcaat ttgttagtgc cggatcggtt aaatcattaa
      241 aaaaagattt aggattagac attcgggata ttttaaaaac acatttaaga ggataaatta
      301 tgttaatttc attaaatgta aaaaactttg cgattattga taacatgcaa attgaattcc
      361 aagatggcat gacatgtttg accggagaaa ctggggcggg aaaatctttg attatcgacg
      421 ctattggctt attgtttgga aaacgtgcct ctactgattt aattagattc aacgaaacta
      481 aagccacgat tgaaggtgta ttcacaaaat taaaaccgga aatgactgaa atcatcgagc
      541 ttaatcctga tgatgatgtt ctcatcatta aaagagaaat ctatgccaac gggaaaagtt
      601 tgtgtaaagt taataaccaa acggttacat taaatcaatt aaatgattta gcagctctaa
      661 ttggcgatat tcatacccaa tatgatactc aaggtttatt caaccccaag aattacttaa
      721 aatttattga taattcggaa gttttaggac ttattcccag ttatcaagaa aacctaaaaa
      781 agtatcgtga attccaaaat gactatcaga aattattaaa gcgcagtatt gatgattccc
      841 agcgtcttga gtttttaaaa tatcaaatta aagaacttga gcaagcacaa ttatcactag
      901 aagaagaagg acaattacaa caacaagcta attatttaag taattttgaa caaattagca
      961 caaacattaa tgatgtaaaa gaaatttttt ctgaacaaca actccttgac catttatacg
     1021 aagctctttc tttgcttaaa aaggtcagcc aatttgatga gcgttatgat atcatccaaa
     1081 aaaagatgga agaaatttat tataatttaa gcgatcaaat cggtgaaatc aataaattat
     1141 ctaaatcgct tgattttgat ccagcggaat tagaccgcat taatgagcgt ttgggtttat
     1201 attccgaatt gaaacggaaa tacaagaaga atttaactga tttattactt tttcaaaaac
     1261 aaatcagtga agaaatcaat ttaattgaaa actttgat
//
LOCUS       DAOSGN010000323         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_109907_length_1298_cov_1.858407, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000323 DAOSGN010000000
VERSION     DAOSGN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109907_length_1298_cov_1.858407"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..>1298
                     /locus_tag="PK087_04510"
     CDS             98..>1298
                     /locus_tag="PK087_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOH18567.1"
                     /translation="MLILVIYIKAYCKRRKIVIELLAPAGDLEKLKVAILYGANAVYI
                     GGKKFSLRARASNFSLEEIKEAVIFAHHHQAKVYITMNIVPHNDDFDGLEDYLQTLDA
                     YGVDGIIVTSLSIAQVAKEITPRLERHLSTQFSASNSSTVNLFKSWGFSRVILAREVS
                     LQQIKAIVEKTDLELEVFIHGGMCVSYSGRCMLSNHMTNRDANRGGCAHSCRLIYQLF
                     DHNLPISEDQDFAMGSTDLCGLRAIPTLIDMGISSLKIEGRMKSIHYIATVVRSYRML
                     IDEYYSTGTIKDFTIYEQEIAKAENRTTGTGFLFHKPGIAEQVYNNNELFPTKEFIGT
                     VLEYNQETGFVKVEQRNFFAVGDVLEIFGPNKAHFQQKVLSLQDELGNEITEAPHPLQ
                     IVYFQVSK"
BASE COUNT          395 a          224 c          252 g          427 t
ORIGIN      
        1 caggcccagt tattagccat tactaagctt tataaacaag gccttaatac tttttttact
       61 gatactcttt atcatgattt agttgctatt tgtcctcatg ttgatactgg ttatttacat
      121 aaaggcttat tgcaaaagga ggaaaatcgt gattgaatta ttagctcccg ccggtgattt
      181 agaaaagcta aaagttgcca ttttatatgg tgccaatgcc gtttatatcg gtgggaaaaa
      241 gtttagccta agggctcgag ccagtaattt tagcctcgaa gaaatcaaag aagctgttat
      301 ttttgcccat catcatcaag ccaaggttta catcaccatg aatattgttc ctcataatga
      361 tgattttgac ggcttggaag actatttaca aacccttgat gcttatggtg tcgatggaat
      421 cattgtcact agtttatcta ttgctcaagt tgctaaagaa ataacgcccc gattagaaag
      481 acatttgagt acccagtttt ctgcttccaa ttccagtact gtcaatttat ttaaatcatg
      541 ggggttttcg cgagttatct tagcaagaga agttagtttg caacaaataa aagcaatagt
      601 agagaaaact gatttagaat tggaagtttt tattcatggc ggcatgtgtg tttcctactc
      661 tgggcgctgt atgctcagca accatatgac gaatcgcgat gctaatcgcg gtggctgtgc
      721 ccattcgtgt cgtttaattt atcaattatt tgatcataat cttccaatta gtgaggacca
      781 agattttgct atgggctcga cggatttgtg tggtctaaga gcgattccca cccttattga
      841 tatggggatt tcctctttaa aaatcgaagg acgaatgaaa tcgattcatt atattgcaac
      901 ggttgtccgc agttatcgga tgttaatcga tgaatattat tcaacaggaa ctatcaaaga
      961 ttttactatt tatgaacaag aaatcgccaa agctgaaaac cgaacgacgg gaacaggttt
     1021 tcttttccat aaacccggga ttgcggaaca agtatataat aataatgaat tatttccgac
     1081 taaagaattt attggcacgg ttttagaata taatcaagaa acaggttttg taaaagtaga
     1141 acaacgcaac ttttttgcgg ttggtgatgt tttagaaata tttggtccca ataaagccca
     1201 tttccaacaa aaagttctat ctttgcaaga tgaattaggt aatgaaatta ctgaagctcc
     1261 tcatcctttg caaattgttt attttcaagt ttctaagc
//
LOCUS       DAOSGN010000324         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_110643_length_1292_cov_3.605497, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000324 DAOSGN010000000
VERSION     DAOSGN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110643_length_1292_cov_3.605497"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>1292
                     /locus_tag="PK087_04515"
     CDS             36..>1292
                     /locus_tag="PK087_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOH18568.1"
                     /translation="MQSAFFKLNEQIMPYEEAKELMKKYAHKSYIKRGQHIVNQNYLA
                     IDKGAESLKEIKVDPAWSKLPAEEKVVDLKVPPFVTKIANPVNALRGDDLPVSVFNGY
                     EDGTMENGTAAFEKRYIAPFVPMWIEENCIQCNQCAFVCPHAVIRPFLSTADEVKNAP
                     EQAKYLDPVGKGLEGLKYSIVVSVADCTGCEVCVNQCPGKKGEKALKMVPLDEQVHQG
                     QDQVAKYFHEHVSYKDNILDKFANPKNSQFAKPLFEFSGACAGCGETPYVRLLTQLFG
                     DHMMVANATGCSSIYSGSFPTTPYTKNAEGKGPAWANSLFEDNAEFGYGMRIATETIR
                     HRMGVMMEKILEKEGQANRMHELLQAWLDKRDNTVETEKIAQELVPLLEKAKGEQAKE
                     LFKLKDYLVKRSQWIIGGDGWAYDIGF"
BASE COUNT          408 a          232 c          296 g          356 t
ORIGIN      
        1 tggaattagg acttggaaat cgcacgaaca caattatgca atcagcgttc tttaaactga
       61 atgagcaaat tatgccttat gaagaagcta aggaattaat gaagaaatat gctcataaat
      121 catatattaa aagaggccaa catattgtca atcaaaacta tttagcaatt gacaaaggtg
      181 ctgaaagctt aaaagaaatt aaagttgatc cagcttggag taaacttccg gcggaagaaa
      241 aagttgttga tttaaaagtg ccgccttttg ttacgaagat tgccaatcca gtcaatgctt
      301 tgcgaggcga tgatttaccg gtttcggttt tcaatggtta tgaagacgga acaatggaaa
      361 acgggacagc cgcttttgaa aaacgctata ttgcaccttt tgttccgatg tggattgaag
      421 aaaactgtat tcaatgtaat caatgtgctt ttgtttgtcc gcatgcggtc attcgtccgt
      481 tcttgtcaac agctgatgaa gtaaaaaatg cccctgaaca agcaaaatat ctggatccgg
      541 ttggcaaagg cctagaaggc ttaaaatatt ccattgttgt ttccgttgct gattgcacgg
      601 gttgcgaagt ttgtgtcaac caatgtccgg gtaaaaaagg tgaaaaagcc cttaagatgg
      661 tgccgcttga tgaacaagtg catcaaggcc aggatcaagt tgccaagtac ttccacgaac
      721 atgtttctta taaagacaat attttagata aatttgccaa cccgaagaat tctcaatttg
      781 cgaaaccctt gtttgaattt agcggtgctt gtgccggttg cggagaaact ccatacgttc
      841 gcttattaac acaattattc ggggatcaca tgatggtggc taatgctacc ggatgttctt
      901 cgatttatag tggctccttc ccaaccactc cttataccaa gaatgcggag ggcaaaggtc
      961 cagcttgggc caattcctta tttgaagaca atgcggaatt cggttatgga atgcgcattg
     1021 ccactgaaac catccgccat cgtatgggtg tgatgatgga aaagattctt gaaaaagaag
     1081 gccaagcaaa tcggatgcat gaattattgc aagcatggtt ggataagcgc gacaacacgg
     1141 tggaaaccga aaagattgcg caagaattag ttccattatt agaaaaagcc aaaggcgaac
     1201 aagcaaaaga gttgtttaaa ttaaaagact atttggtaaa acgctcgcaa tggattatcg
     1261 gtggtgatgg ttgggcctat gatatcggtt tt
//
LOCUS       DAOSGN010000325         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_112754_length_1276_cov_2.661753, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000325 DAOSGN010000000
VERSION     DAOSGN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112754_length_1276_cov_2.661753"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PK087_04520"
     CDS             complement(<1..805)
                     /locus_tag="PK087_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HOH18569.1"
                     /translation="MINQNSYQAVMARKNEIMKKAVGIDYSLYETGKISFDYEKLMRD
                     NGYPLSGIEAIQKAHGVGSTPLKELHNITALARHYCPKGFGARIFIKDEALNDSGSFK
                     ARRASVAVYRAKTLGYKGVIAATSGNYGAAVASQAAKYGLKCIVIQECYDSHQVDQPE
                     IIEKARACEAYGAEVIQLSVGPELFYTFLRLLEETGYFNASLYTPFGIAGIETLGSEL
                     VQDCLVQTKKLPNMVVCTNAGGGNLSGTARGIKKATDKAITIVGASVNLK"
     gene            complement(798..1103)
                     /gene="ortA"
                     /locus_tag="PK087_04525"
     CDS             complement(798..1103)
                     /gene="ortA"
                     /locus_tag="PK087_04525"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HOH18570.1"
                     /translation="MVIKKNTWVQIEKVVLKPEERAANVPLDTKQVPLLMWVKGYLQN
                     DAQSGDLVSITTLSGRTEEGLLVAENPAFKHNYGDFVPEILQINQMVKKILFGDSHD"
     gene            complement(1100..>1276)
                     /locus_tag="PK087_04530"
     CDS             complement(1100..>1276)
                     /locus_tag="PK087_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HOH18571.1"
                     /translation="VNLRINPEIDGGKGTIAICVNMIPHVINAKPGLKTMLDLPVPRA
                     IMGDVRQMIDEEER"
BASE COUNT          334 a          285 c          258 g          399 t
ORIGIN      
        1 ctttaaggtt gacactggcg ccgacaattg taatcgcttt gtccgttgct ttcttaatgc
       61 cacgggccgt tccgctaaga ttacccccac cggcattggt acaaacaacc atattgggaa
      121 gtttttttgt ttgcactaaa caatcttgga ctaattcgct tcctaaagtt tcaattccgg
      181 caatcccaaa gggggtgtat aacgaagcat taaaataacc tgtttcttct aacagtctta
      241 aaaaggtata gaataattcc ggaccaacac ttaattggat aacttccgct ccataagctt
      301 cgcaagcgcg agctttttca atgatttccg gttgatcaac ttgatggctg tcataacatt
      361 cttggatgac aatacatttt aaaccatatt tggcggcttg tgaggcgacc gccgccccat
      421 agttaccgct agtggcggcg ataacaccct tgtaacctaa agttttagcc cggtaaacgg
      481 caacagatgc ccggcgcgct ttaaaacttc ccgaatcatt taaagcttcg tctttaataa
      541 aaatgcgggc tccaaatcct ttggggcaat aatgtcgtgc taaagcggtg atgttatgaa
      601 gttctttaag cggggtactg ccgacgccat gagctttttg aatggcttcg attccagaga
      661 gcggatagcc attatcacgc attaactttt cataatcaaa gctgattttt ccagtttcgt
      721 ataatgaata atcaatgccc accgcttttt tcatgatttc gtttttacga gccataacgg
      781 cttgatatga attttgatta atcatgggaa tcaccaaata atattttttt aaccatttgg
      841 ttgatttgta agatttcagg cacgaaatcg ccataattat gtttgaaggc cgggttttcc
      901 gcaacaagaa gaccttcttc agttcgtccg cttagagttg ttatgctgac taaatcaccg
      961 ctttgggcat cattttgcaa atagcctttg acccacatta aaagcggaac ttgtttcgta
     1021 tcgaggggaa cattggcggc gcgctcttcc ggttttaaaa cgactttttc aatttgtacc
     1081 catgtgtttt tcttaatcac tatctttcct cctcgtcaat catttggcgg acatcaccca
     1141 tgatggcgcg gggaaccggt aaatcgagca tcgtttttaa gcctggtttt gcgttgataa
     1201 catggggaat catattgaca caaatggcaa tcgtcccttt tccgccgtca atttccgggt
     1261 taatgcgcaa attaac
//
LOCUS       DAOSGN010000326         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_112911_length_1275_cov_2.520492, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000326 DAOSGN010000000
VERSION     DAOSGN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112911_length_1275_cov_2.520492"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK087_04535"
     CDS             <1..372
                     /locus_tag="PK087_04535"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOH18572.1"
                     /translation="FGQSLLYIGYIIVSCIQLGATIDYGILYASRYLHYRGDYDVKEA
                     TNYAYEDSKTTVLTSGLILICAGYLLGMVSSIPSIAIFGTLIGRGALISTLLVLFVLP
                     QTLAMLDPFVKVTTYRKKRKS"
     gene            494..>1275
                     /locus_tag="PK087_04540"
     CDS             494..>1275
                     /locus_tag="PK087_04540"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH18573.1"
                     /translation="MNDDLLLQIKTIIETSTDPENLREQLVNFHPYDLAAAFKLLDED
                     LRQKIYQTLTPEQLADLFEYIESDETADFLEEMGPQKSASVLAEMEADDATDVLQEIQ
                     GEQKEAYLSQIEPETKEELTYLTSHPEDTAGAVMTTNYIALERTLDVKQAMKILVKEA
                     NESEIIDPLFIIDQDHLVGTVSLKDLIIARSPKMLEEIMTPNPITVDVGDEAKLAVNK
                     INDYALEALPVLDQGKMVGIITIDDAMDVIDDSASEVYSLFAG"
BASE COUNT          416 a          244 c          244 g          371 t
ORIGIN      
        1 ttcggccaaa gccttctcta tattggttat atcattgtca gttgtatcca actaggagca
       61 accattgatt atggaatctt gtatgctagt cgctatttac actaccgcgg tgattatgat
      121 gttaaagaag cgacgaatta tgcctatgaa gattcgaaaa caaccgtttt aacttccggt
      181 ttaattttaa tctgtgcagg atatttatta ggaatggttt ccagtattcc atccattgcc
      241 atctttggca cattaatcgg ccgtggggct ttaatctcca ctttattagt gctgtttgtg
      301 ctaccacaaa ctctagcaat gcttgatccg tttgtgaagg tcactactta ccgaaaaaaa
      361 cgaaaaagct aagttaataa gataaaccga aacgagttct ttttctcaag aaaacttgtt
      421 tcggtttttt tattttctac tggaaaaaaa acaaaaaaag gtttataata ggtaaataaa
      481 ggaggaaata gccatgaatg atgacttgtt attgcaaatt aaaaccatta ttgaaacaag
      541 tacggaccca gagaatttac gtgaacaact agtgaatttc catccttacg atttagctgc
      601 tgcttttaag cttcttgatg aagatttacg ccaaaaaatt tatcaaacct tgacgcctga
      661 acaattagct gatttattcg aatatatcga gtcggatgag actgccgatt tcttagaaga
      721 gatgggaccg caaaaaagcg cctccgtact tgcggaaatg gaagctgacg atgccaccga
      781 tgttttacaa gaaatccaag gcgaacaaaa agaagcttat ttatcacaaa ttgaaccgga
      841 aacaaaagaa gaattaactt acctaacttc ccaccctgag gatacggccg gtgcggtgat
      901 gaccacaaat tatatcgctc tagaaagaac gcttgatgtt aaacaagcca tgaaaatttt
      961 ggtaaaagaa gccaatgaga gcgaaatcat cgatcctctt tttattatcg atcaagatca
     1021 cttagtcgga actgtttctt taaaggatct catcattgcc agaagcccaa aaatgttaga
     1081 agaaatcatg actcctaacc cgattacagt cgatgttggt gatgaagcca aattagccgt
     1141 caataaaatc aatgattatg ctcttgaagc tctacccgtc cttgatcaag gaaaaatggt
     1201 cggcattatc accatcgatg atgccatgga tgtcattgac gacagcgctt cagaggtcta
     1261 tagcttattt gctgg
//
LOCUS       DAOSGN010000327         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_113088_length_1274_cov_2.058244, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000327 DAOSGN010000000
VERSION     DAOSGN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113088_length_1274_cov_2.058244"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..>1274)
                     /locus_tag="PK087_04545"
     CDS             complement(109..>1274)
                     /locus_tag="PK087_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18574.1"
                     /translation="RVFFLKDAMADLKTTAIEALLLKNHHLVSQELVKVYDHYALRKT
                     IWEFLGSVLIAFIYPGVLGIVAYYAFIDLQIAAFASLTVAATTIANLISRLVNILANI
                     QSEMIESRVPFIFLSMTSKIEGRGGQRVGDFQSLKIKHLDFGYDEKIVLHDLNMEIKQ
                     GDKIAIVGANGAGKTTLVKLLLRLYDCSKGEIVYNGEPYPSLDPSSLRKKVGAVFQNP
                     EVYSVTIGENVLLKPVETKEEEQLVIDALKFSGLYDDVMRYEEGIQTLVTREFQIKGA
                     IFSGGQMQKLAIARGFAQNYQLFILDEPSSSLDPLAEAKLYQNMLELGKDKTLLFISH
                     RLSTTINCDYIYLFENGIIVEAGKHDDLMKLKGQYWAMFNSQSEKYLGGEQID"
BASE COUNT          422 a          270 c          213 g          369 t
ORIGIN      
        1 aaaaccgcac tgagggcatt agcagcaata taaaaaagcg aaataacgac acaaagcgga
       61 caaaaggaaa agacataacg gatgattttc acattcttaa aaaaggattt aatcaatttg
      121 ttcacctccc aaatactttt cggactggga gttaaacatg gcccaatatt gtccttttaa
      181 tttcattaaa tcatcatgct tccccgcttc cacaattatc ccgttttcaa acaagtagat
      241 atagtcacaa ttaatcgttg tgctgagacg atgagaaatg aataataggg ttttgtcttt
      301 acctaattcc aacatatttt ggtaaagttt ggcttccgcc aaggggtcca aagaacttga
      361 gggttcatca agaataaaca gttgatagtt ttgcgcaaac ccacgggcaa ttgccagttt
      421 ttgcatttga ccgccggaaa aaatagcgcc tttaatttga aattctcgtg ttaccaaagt
      481 ttgaatgccc tcttcataac gcataacatc atcataaaga ccgctgaact ttaaagcatc
      541 aataactaat tgttcttctt ctttcgtttc caccggtttc aaaagaacat tttcgccgat
      601 tgtcacacta taaacttctg gattttgaaa aacagcgccg acttttttgc gtagtgatga
      661 aggatcaaga gaaggatatg gttcaccatt ataaacgatt tcacccttgc tgcaatcgta
      721 taagcgcagc aacaacttca caagcgtcgt tttcccggcg ccattagccc caacaatggc
      781 aattttatcc ccttgtttaa tttccatatt gagatcgtga aggacaatct tttcatcata
      841 gccaaaatca agatgcttga tttttaaact ttgaaaatca ccaacccttt gaccaccccg
      901 gccttcaatt ttcgatgtca ttgataaaaa aataaagggt actcggcttt caatcatttc
      961 actttgaatg tttgctaaaa tattgactaa acgggaaatt aaattggcaa tcgtcgtcgc
     1021 tgccaccgtc agtgaagcaa aagcggcaat ctgtaaatca ataaaagcat agtaagcaac
     1081 aatccctagc acaccaggat aaataaaggc aattaaaaca cttcctaaaa actcccaaat
     1141 tgttttccta agggcataat ggtcatagac tttaactaat tcttgactga caagatgatg
     1201 atttttaagg agcaaagcct caatggcagt tgtttttaaa tccgccatcg catcctttaa
     1261 aaagaacact cgat
//
LOCUS       DAOSGN010000328         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_113090_length_1274_cov_2.023790, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000328 DAOSGN010000000
VERSION     DAOSGN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113090_length_1274_cov_2.023790"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PK087_04550"
     CDS             complement(<1..237)
                     /locus_tag="PK087_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOH18575.1"
                     /translation="MLNKDGVPSLDQIKTCFPNLESLQKPKAIIECYEEIPCNPCSTS
                     CPFKAIYIGPNINKRPQVDFNKCTGCGVCVYSCPG"
     gene            complement(227..1274)
                     /locus_tag="PK087_04555"
                     /pseudo
     CDS             complement(227..1274)
                     /locus_tag="PK087_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
BASE COUNT          365 a          316 c          238 g          355 t
ORIGIN      
        1 acccgggcaa ctgtaaacac acacgccaca acccgtacat ttattaaaat cgacttgggg
       61 acgcttatta atgttggggc caatataaat cgccttaaaa gggcaactgg tactacaagg
      121 attgcaggga atttcctcat agcattcaat gatggcttta ggcttttgta agctctctaa
      181 attaggaaaa caagttttga tttgatctag gcttggaaca ccatctttat ttaacatctt
      241 ctaaacctcc cagtttggcc aagccttggc gaattttgac cccaaaaggt ccttcccgaa
      301 ggatgcgaag ttgttcttga tagtcagcaa ctaatttttg atgatgagga tggcttttac
      361 cgagtttata acaagctgct aaaccggcta aatttccctc gacaatcgca ctgctggctt
      421 cttcaatgcc cgcggcatca ccacaaacaa ataaatgggg aatgctggtt tcatagtagt
      481 cattggtcat cggcactaag ccaccaagtt aactgacata tttcatcttc acacccatca
      541 tccctaaaac attggtgagt ggcgtcaaac caacagcaat acaaaccgca tccacggcga
      601 actcacgctc acttccagga atctcttgcc acttctcatc taaggcaacc gtgattattt
      661 tctcaacagc ttctttgcca agggcttttt taatagttcg tgaagtatag atttccacac
      721 ccaagcgtct aagtttcgaa gcatggactt tatagccgcc aatggtcggc gctgcttcca
      781 agatacatac tacctcaaca ccggcttgca ataattgata actgacaatt aggccgatgt
      841 tgccactccc caccatcacc acttggtgcc ccggtttaac cccatagaca ttcattaagg
      901 tttggactgc accagcgcca taaattcccg gtaaatcatt attttcaaaa gccagcactt
      961 tttcactggc accggtagcg acaatgatgg cttccgcttg aactttcaaa tattgattat
     1021 ggcgataaac cgttacaaca aaatcaggat ataaaccgac aaccgtggca tcggtccaaa
     1081 tttcgaccaa ttgcgaggct tgttccaatt ccgctaggaa gtttcgcgca atttgaaaac
     1141 ctcttgtttt agcatattgt ttttctgaac caaaaaacat atgcgtttgt ttgattaact
     1201 ggccacctaa ataaggattg cgatcaatta acaacaccgg ttgaccggct tcagcagcaa
     1261 ttttggctgc acat
//
LOCUS       DAOSGN010000329         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_113176_length_1273_cov_3.027915, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000329 DAOSGN010000000
VERSION     DAOSGN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113176_length_1273_cov_3.027915"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PK087_04560"
     CDS             <1..945
                     /locus_tag="PK087_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH18576.1"
                     /translation="LEKRIIPVALNIATLCYDDETGSFECAAGAEQRLFAVPISIESV
                     TIYYNKALVQEADLPKTFEDFLADSAAYRAAHEGQRYFATGSHWADAYFLQGFYGAFG
                     WTPFGPNLNDATQVGFEDPKSLRAMEWMINNLKPEVTGTGTHNSINGSTLFEEGKLPF
                     ILTGPWNVPQYLKAGIDLGNITLPSINGVNPKTFAGAQMVSVYKYSKNADAAMKFVEF
                     LQTDQAAEILYRTSGDCPALKDEILATITGIKDDKLISVMLDQLSTSVPMPTIPEVTY
                     YWGPAESMMKNIWNADGVIATEAFTAEKAYKASRDMAS"
     gene            1008..>1273
                     /locus_tag="PK087_04565"
     CDS             1008..>1273
                     /locus_tag="PK087_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18577.1"
                     /translation="MKGKIIHILKAIASGIIWGLGQVFNRQYWKALFFFAFFGILVGI
                     EVGTSRYLSGYSPYEKVPGVDFNQNFINRFVSSYKVDCFDGMPA"
BASE COUNT          364 a          248 c          275 g          386 t
ORIGIN      
        1 ttggaaaaac ggattatccc ggttgcttta aatattgcga cattatgtta cgatgatgaa
       61 actggtagct ttgaatgtgc tgccggggcc gagcaacgac tctttgcggt cccaatttcg
      121 attgaatcag tcaccattta ctacaacaaa gctttagttc aagaagctga tttaccgaaa
      181 acctttgaag attttttagc tgattcggct gcttatcgag ctgctcacga aggacaacga
      241 tattttgcta ccggctcaca ctgggcggat gcttatttct tgcaaggctt ttatggagcg
      301 tttggctgga caccgtttgg accgaatctc aatgatgcga ctcaagtggg ctttgaagat
      361 ccaaaatccc tccgtgctat ggaatggatg atcaataatt taaaaccgga agtaacggga
      421 acaggaacac acaactccat caacggttca actttgtttg aagaaggaaa attacccttt
      481 attttgactg gaccatggaa tgttccgcaa taccttaagg ccggaattga tttaggaaat
      541 attacgcttc cttccatcaa cggtgttaat ccaaaaacct ttgctggtgc gcaaatggtt
      601 tccgtttaca aatattctaa aaatgccgat gcggccatga agtttgtgga gttcttacaa
      661 accgatcaag ccgctgaaat cctctatcgt acttcgggcg attgcccagc tttaaaagat
      721 gaaatcttag caactattac cggtattaaa gatgataaat taatttccgt catgttggat
      781 caattatcga cttccgtacc gatgccaacg attccagaag taacttatta ttggggtccg
      841 gcggagtcca tgatgaaaaa catttggaac gctgatggag taattgcgac agaagcattt
      901 acggctgaaa aagcttataa agccagtcga gatatggcat cttaatgaac aaaagtgaaa
      961 taaggtggcc ttaattggcc acctatttcc ttatttggag gtaaagagtg aaaggaaaaa
     1021 taattcacat tcttaaagcc attgcttcgg gaataatttg gggtttaggc caagttttca
     1081 atcgccaata ctggaaagct ttattctttt ttgctttttt tgggattctt gtgggcattg
     1141 aagttggcac gagtcgttat ctatccggct attctcctta tgaaaaagtt cccggtgtcg
     1201 attttaacca aaactttatt aatcgtttcg ttagttccta taaagtcgat tgttttgacg
     1261 gcatgccggc aat
//
LOCUS       DAOSGN010000330         1267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_113979_length_1267_cov_2.397690, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000330 DAOSGN010000000
VERSION     DAOSGN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113979_length_1267_cov_2.397690"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PK087_04570"
     CDS             <1..665
                     /locus_tag="PK087_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOH18578.1"
                     /translation="LLEAKTKMDEAYQETLKPANIISVLRQGLAKTLVAQIITNLNDL
                     QLRNTSFEIIFSNPKTLVFNQDGIDNVDFLVSFNPGEPLKPLSKVASGGELSRFMLAL
                     KTIVCDKLALQTIIFDEIDHGVSGAIAFSIANKIKTIAKNAQVLCVTHLPQVAAIADQ
                     HLHITKHLEDGRTLTKISQLDNEDRTLEIAKMISNGIATTASLNLAKELLCSHQIKNG
                     SR"
     gene            complement(685..861)
                     /locus_tag="PK087_04575"
     CDS             complement(685..861)
                     /locus_tag="PK087_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOH18579.1"
                     /translation="MEPKEQVYQAMQQAKQAVSVGEIAKATGLDRKVVDKAFNDLKKD
                     GKIVSPIRCKWQAK"
     gene            complement(889..1104)
                     /locus_tag="PK087_04580"
     CDS             complement(889..1104)
                     /locus_tag="PK087_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18580.1"
                     /translation="MKKVKKTEKAPKKTPFIKTKVRPNNSVEVEIQKNPAQTLLGKIL
                     IYVLVIGMTLLGAVALIYLMIQVGKTI"
BASE COUNT          378 a          239 c          211 g          439 t
ORIGIN      
        1 tgttgcttga agctaaaaca aagatggatg aggcttatca ggaaacttta aagccagcta
       61 acatcattag cgtccttcgc caaggattgg caaaaacatt agttgcgcaa ataattacta
      121 atttgaatga tttgcaactc cgtaatacct cttttgaaat tattttttcc aatccgaaaa
      181 cccttgtctt taatcaagac ggaattgaca atgttgattt tctcgtttca tttaatcctg
      241 gtgaaccact taaacccctt tctaaagttg cttccggtgg tgagttgtcc cgtttcatgt
      301 tagctttaaa aactatcgtt tgtgacaaat tagctttaca aactattatt tttgatgaga
      361 ttgatcatgg tgtttcgggg gcgattgctt ttagtattgc taataaaatt aaaaccattg
      421 caaaaaatgc acaggttctt tgtgtcactc atcttccgca agtagcggcc attgctgatc
      481 aacatttgca tatcacgaaa catttggagg acggtcgaac cctaaccaaa atttcgcaac
      541 ttgataatga agaccgaacg ctagagattg caaaaatgat ttctaatggt atagcaacaa
      601 cggccagtct taatcttgcg aaagaactat tatgttcgca ccaaataaaa aacggatcaa
      661 gataacttga ttcgttttgt ttgctcattt ggcttgccat ttacaacgga ttggggaaac
      721 aattttgccg tcctttttta aatcgttaaa agccttatca actactttac gatctagtcc
      781 agtcgctttt gctatttcac cgacactaac agcttgttta gcttgttgca tggcttgata
      841 tacttgttct tttggttcca tgataattcc tccaatcttt ggtaattatt aaattgtttt
      901 gcctacttga atcattagat agattagagc tacggcgcct aataaggtca taccaattac
      961 caaaacataa attaagattt ttccgagtaa agtttgtgcg ggatttttct gaatttctac
     1021 ttccacagaa ttattcggtc taactttggt tttaataaac ggggtttttt ttggagcttt
     1081 ttccgtcttc ttcacttttt tcacttttct cacctcgaca tttatgatat aataataaca
     1141 tgtttattgt atcacacaat ccccaaaaag gaaataaaaa aacatgaaaa gccccaaaat
     1201 tattttccat atcgatttaa atagtttctt tgcttcttgt gaaatggcgg aagaccccga
     1261 gttaata
//
LOCUS       DAOSGN010000331         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_114284_length_1265_cov_2.657851, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000331 DAOSGN010000000
VERSION     DAOSGN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114284_length_1265_cov_2.657851"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /gene="grpE"
                     /locus_tag="PK087_04585"
     CDS             <1..461
                     /gene="grpE"
                     /locus_tag="PK087_04585"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOH18581.1"
                     /translation="AQEQIKKLNDLYLRSLADAENFKKRINEERIRERKYGSQALLEK
                     LVEALDVFDRVVSIKTDDEKLKSFLQGFEMINQQLKQIVSDEGVKKLESLYKPFDPAF
                     QHAVEVGSNPNYDDNIVISEIQSGYMFKDRVLRPALVVVNKKNQEEKKNE"
     gene            454..>1265
                     /locus_tag="PK087_04590"
     CDS             454..>1265
                     /locus_tag="PK087_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003393891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOH18582.1"
                     /translation="MSKIIGIDLGTTNSVVSVMEGGEPKIIVNADGGRTTPSVVAFKD
                     DEILVGEVAKRQAITNLNTVSSIKRLMGSNERIEVNGKKYTPQEISAMVLQNLKATAE
                     AYLGEKVTDAVITVPAYFNDAQRQATKDAGKIAGLNVRRIINEPTAAALAYGMDKKDK
                     EQTVLVYDLGGGTFDVSILSLADGTFEVISTAGNNRLGGDDFDKVVMDYLIDEFRKHE
                     GIDLSKDRMALQRLKDAAEKAKKELSGITKAEISLPFISMRDGNPVHLNSSLT"
BASE COUNT          426 a          195 c          282 g          362 t
ORIGIN      
        1 aagctcaaga acaaataaaa aaactgaatg atctttattt gcgttctctt gcggatgcgg
       61 aaaacttcaa aaaacggatt aatgaagaac gcattcgtga acgaaaatat ggatcgcaag
      121 ctttgcttga gaaactagtg gaagcccttg atgtgtttga tcgagttgtt tccataaaaa
      181 ccgatgatga gaaattaaaa tcttttcttc aaggttttga aatgatcaat caacaattaa
      241 aacagattgt cagtgatgaa ggtgtgaaaa aactagaatc gctttataaa ccatttgatc
      301 ctgcttttca acatgctgtg gaagtgggat ccaaccctaa ttatgacgat aacattgtta
      361 tcagtgaaat ccaaagtgga tatatgttta aagatcgggt tttgcgcccc gctttagttg
      421 tagtaaataa aaagaaccag gaggaaaaaa agaatgagta aaattatagg aattgatttg
      481 ggaacaacca attcagttgt atcagtaatg gaaggtggag aaccgaaaat aattgttaat
      541 gccgacggtg gaagaacgac cccatcggtt gttgctttta aggatgatga gattttagtt
      601 ggtgaggttg ctaagcgaca agccattact aatttaaaca ctgtttcatc aattaaaaga
      661 ttaatgggtt ccaatgaacg gattgaagta aatggaaaaa aatatacgcc tcaagaaatt
      721 tcggcgatgg ttttacaaaa tttaaaggct actgcggaag cctatttggg agaaaaagtt
      781 accgatgcgg ttattaccgt tccggcttac tttaatgacg cccaacgtca agcaacaaaa
      841 gatgctggga aaattgccgg tttaaatgtt cgtcgcatca tcaatgaacc aaccgctgcc
      901 gctttagctt atgggatgga taaaaaagat aaggaacaaa ctgttttggt ttatgacctt
      961 ggcggtggaa cttttgacgt ttccattttg tctttggcgg atggaacatt tgaagttatt
     1021 tcgacggcag gaaataaccg tttaggtggt gacgattttg ataaagtcgt tatggattat
     1081 ttaattgatg aatttagaaa acacgaaggc attgatttaa gcaaggaccg catggcctta
     1141 caacgtctca aagatgctgc tgaaaaagcg aaaaaagagt tgagtggaat tacgaaagcg
     1201 gaaattagtt taccgtttat ttcgatgcgt gatggcaatc ctgttcacct aaactcttcg
     1261 ttaac
//
LOCUS       DAOSGN010000332         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_114434_length_1264_cov_2.647643, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000332 DAOSGN010000000
VERSION     DAOSGN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114434_length_1264_cov_2.647643"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PK087_04595"
     CDS             <1..1192
                     /locus_tag="PK087_04595"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOH18583.1"
                     /translation="KKVTWKRCVDLNDRALRKVMVAMESKKEVPREDGFNITVASEIM
                     ASLCLSSNYHDLRERLDRMIVAYNLDDQPVTIKDLGITGSLLVLLKNALKPNLIQTLE
                     NNLFICHGGPFANIAHGCNSVLATKLALKLADYVVTEAGFGVDLGAEKFLNIKCQEAK
                     LNPSLVVIVATVRALKYHGGMAVADLKTPNVEALKKGIVNLERHLETIRKFNLPAVVA
                     LNRFASDSEEELSFIMEWGKAHEVDVALSEVFAKGSAGGEDLTNKVLKNMAPRRKYRP
                     LYHISESLFTKIERICVEIYHATNIEYAPEVFEQISKYNQGPYHDFYVCMAKAQTSIT
                     DNPKIVGVPKNHTIHIKEVRLATGSKFLICLTGTIMTMPGLNKEPQAKKIDMDDDGHI
                     TGLM"
BASE COUNT          383 a          232 c          287 g          362 t
ORIGIN      
        1 taagaaagtc acttggaagc gctgtgtgga tttaaatgat cgtgctttaa gaaaagtgat
       61 ggtcgcaatg gaatccaaaa aagaagtccc acgggaagat ggttttaata tcaccgttgc
      121 ttcggaaatc atggccagtt tatgtttatc aagtaattat catgatttac gcgaacgttt
      181 ggaccggatg attgtggctt ataatctcga tgatcaaccg gtaactatta aagatttagg
      241 gataaccggc tccttgttgg tcttattgaa aaatgccttg aaacctaatt taatccaaac
      301 cttggaaaat aatctcttca tttgtcatgg cggtccgttt gcaaacatcg ctcatggctg
      361 caattcggtg ctcgccacta aattagccct caaattagcc gattatgtgg ttacggaagc
      421 cggtttcggt gttgatttgg gtgctgagaa attcttgaat atcaaatgtc aagaagctaa
      481 actgaacccg agtctcgttg tgattgtggc gacggttcgg gcccttaaat atcatggcgg
      541 tatggcggtt gcggatttga aaacgccaaa tgtggaagcc ttgaaaaaag ggattgtcaa
      601 cttagagcgt catttagaga caatacgtaa atttaatttg ccggccgtgg ttgctcttaa
      661 ccgctttgcc tctgatagtg aagaagaact tagcttcatc atggaatggg gaaaagccca
      721 tgaagttgat gttgctttaa gcgaagtctt tgcaaagggc tctgccggtg gggaagactt
      781 aacaaataaa gttttaaaga atatggcccc gcgccgtaaa tatcgtcctc tatatcatat
      841 ctctgaatcc ttatttacca aaatcgagcg catttgtgtg gaaatttatc atgccaccaa
      901 tattgaatat gctccggaag tctttgaaca aatcagcaaa tataatcaag gaccctatca
      961 tgatttctat gtgtgcatgg ctaaagcaca aacctcgatt accgataatc cgaaaatcgt
     1021 cggggtgccg aagaaccata ccatccacat caaagaagtt cgtttggcca ccggttccaa
     1081 attcttgatt tgtttgaccg ggacgattat gacaatgccg ggattaaaca aagaacccca
     1141 agctaagaag attgatatgg atgatgatgg tcatattacc ggattaatgt aagcagtgaa
     1201 aacggttaac aaaccgggta gtgtaaagtt ttattgggta aataataaat gagatactta
     1261 ttag
//
LOCUS       DAOSGN010000333         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_114599_length_1263_cov_2.548013, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000333 DAOSGN010000000
VERSION     DAOSGN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114599_length_1263_cov_2.548013"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..113
                     /locus_tag="PK087_04600"
     CDS             21..113
                     /locus_tag="PK087_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18584.1"
                     /translation="MAIINHKVGDRVLVKTETGNEFYVTIKKIS"
     gene            124..>1263
                     /locus_tag="PK087_04605"
     CDS             124..>1263
                     /locus_tag="PK087_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOH18585.1"
                     /translation="MKYVPSLDKQFIPMIIKLNEFQKQVQAATVKQKVVICVERNNGF
                     NHIYQIDTFKDFTGHDLENYAVVERIIKTLLWLVGGFKIYIAGSEYLFEAIHKDYQPG
                     GKRDFDFHFLANAYEHPFSVVKVDYHEIPPLKQEQISFDANLNGCRIGFDAGGSDRKV
                     SAVVDGKVVYSEEVAWLPKLNADPSYHYQGILEAMNTAASHMPRVDAIGVSSAGIYVN
                     NKVMVASLFLKVPKDLFDAHVKNMYIDIAKTFGDVPLVVANDGDVSALAGAMELKDNQ
                     ILGIAMGTSEAAGYVDEHGSLKGWLNELAFVPVDFNPASMVDEWSGDYGCGVKYFSQD
                     SVIKLALAGGIHFDEQLTLAQKLKYIQKLNDENHPVAQQIFESIGV"
BASE COUNT          381 a          210 c          269 g          403 t
ORIGIN      
        1 ccaactaagc tcctttagga atggccatca tcaaccataa agttggtgat cgtgttcttg
       61 taaaaacgga aacaggtaac gaattttatg ttaccataaa aaaaattagt tgaggtgaga
      121 aagatgaaat atgttccgtc gctagataaa caatttatac caatgattat taagctgaat
      181 gaatttcaaa aacaagttca agcagcaact gtcaaacaaa aggttgttat ttgtgttgaa
      241 aggaataatg gttttaacca tatttatcaa atcgatactt ttaaagactt tacgggacat
      301 gatttagaaa attatgccgt tgttgaaaga atcattaaga ctttgctgtg gttagtcggt
      361 ggttttaaaa tctatattgc cggttcggag tatctttttg aagctattca taaagattat
      421 caacccggag gaaaacgcga ttttgacttt cattttcttg ccaatgctta tgaacatcct
      481 ttttcggttg tcaaagttga ttatcacgaa attcctcctc ttaaacaaga acaaatttcc
      541 ttcgatgcta atttaaatgg ttgtcgaatt ggctttgatg ctggcggttc ggatcgcaaa
      601 gtttccgccg ttgttgatgg taaagtcgtt tacagtgaag aggttgcttg gcttccgaaa
      661 ttaaatgcgg acccaagtta ccactatcaa ggaattttag aagccatgaa cacagcggcc
      721 agccacatgc cacgcgttga tgccattggt gtttcttcag caggaattta tgtcaataat
      781 aaagtgatgg tcgcttcgtt gtttttgaaa gttcccaagg atctttttga cgctcatgta
      841 aaaaatatgt atattgatat tgcaaaaact tttggcgatg tccctttagt agttgccaat
      901 gatggtgatg tttccgcact tgccggagca atggaattaa aagataacca aatattgggg
      961 attgccatgg ggacatcgga agctgccggt tatgtcgatg aacatggttc attaaaaggc
     1021 tggttaaacg aattggcctt tgttcctgtt gattttaatc ctgcaagtat ggttgatgag
     1081 tggagcggcg attatggctg cggtgtcaaa tatttttctc aggattcggt tatcaaactt
     1141 gctttagccg gtgggattca ttttgatgaa cagttgacgc tagcacaaaa actaaaatat
     1201 attcaaaaat taaacgatga gaaccacccc gttgctcaac aaatctttga aagcattggt
     1261 gtt
//
LOCUS       DAOSGN010000334         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_114706_length_1262_cov_3.454847, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000334 DAOSGN010000000
VERSION     DAOSGN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114706_length_1262_cov_3.454847"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /gene="ruvX"
                     /locus_tag="PK087_04610"
     CDS             <1..318
                     /gene="ruvX"
                     /locus_tag="PK087_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOH18586.1"
                     /translation="ERDLAFALNETEKIIREKGIQKIVLGLPKHMSGDVGTLGDYVYE
                     FKKMLEDKLQLEVVLIDERLTSKLANQTMLLADLSRQKRKTKVDKIAATFILQTYLDM
                     KKK"
     gene            335..694
                     /locus_tag="PK087_04615"
     CDS             335..694
                     /locus_tag="PK087_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004438126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOH18587.1"
                     /translation="MENENNNRLTFIDENGNEILCEILFTFESEEFKKNYVLFYPVGS
                     DEEEIEIMAASYVDDENGEGELTAVETEEEWAMIERVLQQFEEEQGYNRRGCEDCDCE
                     DDDCEADEDGHCHCSDK"
     gene            complement(740..>1262)
                     /locus_tag="PK087_04620"
     CDS             complement(740..>1262)
                     /locus_tag="PK087_04620"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOH18588.1"
                     /translation="NRFGIELMSTEFFAEIGKPVIWRGAMLNSMINNFFYEVNWSKDI
                     EYMIIDAPPGTGDIALDLRTIVPSAEVIIVTTPHLAASHVAIKAGVASKMLKHELIGV
                     IENMSYYWNPHSKTKDFIFGKGGGQEVAEKLETELIAQIPINQPLHHLSLYESDEEIG
                     RIYDDLATLLIIR"
BASE COUNT          430 a          201 c          264 g          367 t
ORIGIN      
        1 gaacgcgatt tagcttttgc acttaatgaa acggaaaaaa tcatccgcga aaaagggata
       61 cagaaaattg ttttgggctt acccaaacat atgagtggcg atgtgggaac tttgggtgat
      121 tatgtctatg agtttaagaa gatgctcgaa gataaattgc aattggaagt tgttttaatt
      181 gatgaacgat taacttcgaa attggctaat caaacgatgt tgttggctga tttaagtcga
      241 caaaaacgga aaaccaaggt tgataaaatt gcagcgacct ttattttgca aacttattta
      301 gatatgaaaa aaaagtagaa aggagaatga aaaaatggaa aatgaaaaca acaaccgctt
      361 aacctttatt gacgaaaatg gcaatgaaat tttatgcgaa atattattca cttttgagtc
      421 ggaagaattt aagaagaatt atgtcttatt ttatccagtt ggttccgatg aagaagaaat
      481 tgaaattatg gccgcctctt atgttgatga cgaaaatggt gaaggcgaat taacagctgt
      541 agaaaccgaa gaagagtggg cgatgattga aagagtcctt caacaatttg aagaagaaca
      601 aggatataat cgtcgtggat gtgaagattg tgattgtgaa gatgatgatt gcgaagcaga
      661 cgaagatggt cattgtcact gtagcgacaa ataaaaaaaa tgcttaacga ttgattcgac
      721 atcgttaagc ttttttaaat tatctaataa ttaatagagt ggctaaatca tcataaatcc
      781 gaccgatttc ttcatcactt tcgtacaaag ataaatgatg aagtggttga ttgataggaa
      841 tttgcgcaat caattccgtt tccagctttt cggcaacttc ttgcccgcca cctttaccaa
      901 aaataaaatc tttggttttg gaatgagggt tccaataata ggacatattt tcaattacac
      961 caattagctc atgtttaagc attttggaag caacaccggc tttgatggcg acatggctgg
     1021 cggctaggtg cggcgtcgta acaatgatta cttcggcgct cgggacaata gtgcgcaaat
     1081 ccaaagcaat atcacccgtt cccggtggtg catcaataat catgtattca atatctttac
     1141 tccaattgac ttcatagaag aagttattaa tcatggagtt taacattgcc cctcgccaaa
     1201 taaccggttt gccgatttct gcaaaaaatt cagttgacat taattcgatg ccaaaccgat
     1261 ta
//
LOCUS       DAOSGN010000335         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_115138_length_1259_cov_3.705150, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000335 DAOSGN010000000
VERSION     DAOSGN010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115138_length_1259_cov_3.705150"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PK087_04625"
     CDS             complement(<1..868)
                     /locus_tag="PK087_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH18589.1"
                     /translation="MNEKFLYDFHNTYVTNAYEFLGCHLLEENKYVFRVYAPHADAVS
                     VVGDFNHWDHSKNILKRLTKEGIWEGEITGITDFTNYKYWIKNKNKEIYKQDPYGFHH
                     ETNGSTCSKVYQLNSFSFSDEKWQKSKSHQKIYAGPINIYEVNLGSWRKYPDNEPFNY
                     RKLARELIPYVKKMGYTHIEVMPIMEYPFGGSWGYQVTGYFSVTSRYGLPSDFMYFVN
                     YAHKNGIGVILDWVPAHFPKDDFGLFEFDGQDVYEEPNPLRKEHENWGTRIFNYQKPE
                     VKSFLISSALFFL"
     gene            complement(988..>1259)
                     /locus_tag="PK087_04630"
     CDS             complement(988..>1259)
                     /locus_tag="PK087_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOH18590.1"
                     /translation="DARFIAQLTYLGTHSGRNEDKVGKVGFKPLLCGESVTFEEAKVT
                     FLCKKIYHQNLDEHQIAASIKERYYPRDDFHRFYIGEILKTFVNE"
BASE COUNT          425 a          241 c          184 g          409 t
ORIGIN      
        1 ctaaaaagaa caaagcactg gaaataagaa aggatttaac ttccggtttt tgataattaa
       61 aaatacgcgt tccccaattt tcatgctctt ttcgtaaggg attaggttct tcataaacat
      121 cctggccatc aaattcaaac agtccaaaat catctttagg aaagtgagca ggaacccaat
      181 ctaaaatgac tccaatccca tttttgtgag cataattaac aaaatacata aaatcactcg
      241 gtaaaccata gcgtgaagtg acactgaaat aaccggttac ttggtatccc catgaaccgc
      301 caaaaggata ttccataatt ggcatcactt caatatgggt atagcccatt tttttcacat
      361 agggaattaa ttcacgagca agttttcgat aattaaatgg ttcattatca gggtattttc
      421 gccaagaacc taaattaacc tcataaatat tgattggacc agcataaatt ttttggtggc
      481 ttttggactt ttgccatttt tcatcggaaa aggaaaaaga attaagttga taaaccttgg
      541 aacaagtact tccattcgtc tcatgatgaa aaccataggg gtcttgttta taaatttctt
      601 tattcttatt tttaatccaa tatttataat tagtaaaatc cgtaatccca gttatttctc
      661 cttcccaaat tccttcttta gttaagcgtt taaggatatt tttactgtga tcccaatgat
      721 tgaaatcgcc aacaacgcta acagcatcag catgaggcgc ataaacccga aagacatatt
      781 tattctcttc aagcaaatga cagcctaaga attcgtaggc gtttgtgaca taagtattat
      841 ggaaatcata taaaaacttt tcgttcattt agttctcctt cttaagtcca ttatactatt
      901 taaaccgttt caattcaaca aaaaccaaca agacattttc gaataaataa aagacatgat
      961 attctcatgc cttgtagtat atttttgtta ttcattaaca aaggtcttta aaatctcacc
     1021 aatataaaaa cgatgaaaat catctcgagg atagtagcgt tctttaattg atgccgctat
     1081 ttgatgctca tcaagatttt gatgataaat ctttttacat aaaaaggtca ctttggcttc
     1141 ttcaaaagtc acgctttcac cacaaagaag tggtttgaaa ccaactttcc cgactttatc
     1201 ttcatttctt ccactatgcg tacccaaata agttagctgc gctataaagc gagcatcat
//
LOCUS       DAOSGN010000336         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_115193_length_1259_cov_2.392857, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000336 DAOSGN010000000
VERSION     DAOSGN010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115193_length_1259_cov_2.392857"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1259)
                     /locus_tag="PK087_04635"
     CDS             complement(<1..>1259)
                     /locus_tag="PK087_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18591.1"
                     /translation="NKLYSTYKEMIDYQSEIENYLKRTMKTSYESSTDTVLSSTSSWY
                     TPTLRSYNSGTLHCFILKIAEEVAPNKDDVVDEIKAALFEKELTDTYVATEMVKLRAK
                     NDLLFYDPDLEDAYVKTAKSYKETHTESKAESKTLVAKIGSTEYSADALFNLLDKYYG
                     ITLAYAEINYERMLSNLEFNKIYDYHLQDASDKVRILNADKWESIIIEANNLKNNFVA
                     NAYATYGFSSTYGWKNFIRDVYGANSDHELLYALLYQKVKADYAIKLGDVADISDADA
                     LWTTYTKKMQKMVDEYYKVKGIQMLISVTDTEGNRVDPADWNANQSALAKELYEQIWT
                     YMSEIAGESETKFQDIVKAFQEGPRFLATVAQDLASQPAGFNYVYREIIEVAKFKTAG
                     LNVEFSNLGEYTNTSAVDLAPVEAAKT"
BASE COUNT          363 a          270 c          220 g          406 t
ORIGIN      
        1 cgtttttgca gcctcaaccg gagctaaatc cacagcacta gtattggtat attctcccaa
       61 attactaaat tcaacattta aaccggctgt tttaaatttc gcaacttcaa tgatttcgcg
      121 ataaacataa ttaaatcctg ccggttgcga agctaaatct tgggcaacag tggctaagaa
      181 gcgcggacct tcttggaagg ccttcacaat atcttggaac ttagtttccg attcgccggc
      241 tatttcactc atataagtcc agatttgttc atataattcc ttggccaaag cgctttgatt
      301 ggcattccaa tcagccggat caacgcggtt accctcagta tcggtcacac taattaacat
      361 ttgaatgccc ttaactttgt aatactcgtc caccatcttt tgcatcttct tggtataagt
      421 cgtccataaa gcatcggcat cgctaatatc ggcgacatca cctagtttta tagcgtaatc
      481 agctttcact ttttgataca ataaagcgta caaaagctca tggtcactat tggctccgta
      541 aacatcacga atgaagttct tccacccgta agtggaactg aaaccataag tagcataggc
      601 attggcaaca aagttgtttt tcaagttatt ggcttcaata ataatgcttt cccatttatc
      661 cgcattaaga atgcgaacct tatcggaagc atcttgtaaa tgataatcat aaatcttatt
      721 aaactctaaa ttgcttaaca tgcgctcata gttgatttcc gcataagcca aggtaatccc
      781 gtaatactta tctaaaagat taaacaaagc atcggcggaa tattcggttg aaccaatttt
      841 ggctactaag gtcttgcttt ccgctttgct ttcggtatgg gtttctttat atgatttagc
      901 tgttttcaca taagcatctt ctaaatccgg atcatagaat aagagatcgt ttttagcacg
      961 cagtttaacc atttccgtgg caacataggt atcggttaat tctttttcaa ataaagcggc
     1021 tttaatttcg tcaaccacat catctttgtt cggagcaact tcctccgcaa tctttaaaat
     1081 aaaacaatgc agtgtcccgg agttataact gcggagcgtt ggtgtatacc atgaggaagt
     1141 cgatgacaaa accgtatccg tgcttgattc ataggaagtc ttcattgtcc gcttaagata
     1201 gttttcaatc tcactttgat aatcaatcat ttccttgtaa gtggaataaa gcttgtttt
//
LOCUS       DAOSGN010000337         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_115651_length_1256_cov_2.393006, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000337 DAOSGN010000000
VERSION     DAOSGN010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115651_length_1256_cov_2.393006"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK087_04640"
     CDS             <1..526
                     /locus_tag="PK087_04640"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HOH18592.1"
                     /translation="HESIPGHLLQHVVLKNSDLCNLRKYLSFTSYAEAWAVYVEEFVG
                     KYTKVDPDFIEAYNINKRLTYVILCVADIKINYFGDSITDFGNYLATYFGDLPLEDLE
                     EMYYQLVEEPTNFFEYFFSYYRLMALKDNFMVQAADKNYENPDLEFHKFYLLTGPAPF
                     YILEEQIPIYLNHN"
     gene            618..>1256
                     /locus_tag="PK087_04645"
     CDS             618..>1256
                     /locus_tag="PK087_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18593.1"
                     /translation="MRKIIIGILGLLAISSLKVNALETIVAVEGLGSDEDLIEENYTL
                     VENKVSWDVPGQYQVEYFNYLDNLYETRDVIITNLDNLQQGQSFLSQKTMDIDYSVQG
                     IMKIDSQKTLALGGKNTGYYPYQTQNETVFPYLVLYVNGQVYWERTISDLYYGMIKDA
                     VVTERGITIIGDYDTPNQGRNVFIHEYSFAGQLLLKKEIHGENDDFAHRLFYI"
BASE COUNT          401 a          187 c          248 g          420 t
ORIGIN      
        1 tcatgagtcc atcccgggcc atttattgca gcatgtggtc ttaaaaaaca gcgatttatg
       61 taatttacga aaatacctta gttttacgag ttacgccgaa gcgtgggccg tttatgttga
      121 agaatttgtt ggtaaatata ccaaagttga tcccgacttc attgaagcat ataatattaa
      181 taaacgctta acatatgtca ttttatgtgt tgcggatatt aaaatcaatt atttcggcga
      241 ttctataacg gattttggga attatttggc gacttatttt ggcgacttgc ctttagaaga
      301 tctcgaagaa atgtattatc aactagttga agaaccaact aatttctttg aatatttctt
      361 cagttattac cgattaatgg ccttaaagga taatttcatg gtccaagccg ccgataaaaa
      421 ttatgaaaac ccggatttgg aatttcataa attctatttg ttaactggcc cggctccttt
      481 ctatattttg gaagagcaaa ttcctatcta cttaaaccac aactaaacca aactaaccgt
      541 ttggtttttt taaacgaaaa gacctttttt aacgaaaagg ccgatgatga taataatata
      601 ttggtggagg gattgaaatg agaaagatta tcatcggtat tttgggattg ttggccatta
      661 gttcgctaaa agtgaatgct ttagaaacaa tcgttgcggt ggaaggcttg gggagtgatg
      721 aagatttaat tgaagaaaat tatactcttg tggaaaacaa agtgtcttgg gatgttcctg
      781 gccagtatca agttgaatat tttaactatc ttgacaattt atatgaaaca agggatgtga
      841 ttattaccaa tcttgataat ttacaacaag gtcaaagttt tctttcacaa aaaaccatgg
      901 atattgatta ttcggttcaa gggataatga aaattgactc gcaaaaaacc ttggccttgg
      961 gaggaaaaaa tacgggttat tatccctatc aaacgcaaaa cgaaacggtt tttccctatc
     1021 ttgtgcttta tgtcaatggt caggtttatt gggaaaggac cattagtgat ttatattatg
     1081 gaatgattaa ggatgctgtg gttactgaac ggggaattac gattattggt gattatgata
     1141 ctcccaatca aggacgaaat gtttttattc atgaatattc atttgctggg caattgcttt
     1201 taaagaaaga aattcatggt gaaaatgatg acttcgccca tcgactcttt tatatt
//
LOCUS       DAOSGN010000338         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_115664_length_1256_cov_2.223147, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000338 DAOSGN010000000
VERSION     DAOSGN010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115664_length_1256_cov_2.223147"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PK087_04650"
     CDS             <1..688
                     /locus_tag="PK087_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOH18594.1"
                     /translation="LYGLAENYCKAFTNKKIIITGSGNVGSFCAVKAKALGGKVIAMN
                     DISGMVYDEKGLDVDLVVELKAKHLNLDAYAKMHPQVKFSHNLKDIWDIPGDLVFPCA
                     TQNEIGMEEAKKLVDNKTLLVCEGANMPTTNEATQYLLEHGVLFAPGKAANAGGVATS
                     GLEMSQNAMRFSWTFAEVDQRLKDIMKNIYQNVYQTSLKMGQPGNLVVGANVAGFLKV
                     YEAMKAQGVI"
     gene            690..1073
                     /locus_tag="PK087_04655"
     CDS             690..1073
                     /locus_tag="PK087_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOH18595.1"
                     /translation="MAKKMTDANLKSYSRLVKEPKYLDYLGEFLIDSEQIVDSFKSAK
                     EGVVFTNKRIIIISVSGAFGKKRQFTSYPYHRITTFVVSTAKDLESNAVLDLGYFGTP
                     NLRFEFSGKSEIKTIMKYITEAVIK"
BASE COUNT          402 a          207 c          285 g          362 t
ORIGIN      
        1 tctgtacggt cttgcggaaa actactgtaa agccttcact aataaaaaga ttatcattac
       61 cggtagtggc aatgtcggta gtttctgtgc ggtcaaggct aaagccctag gggggaaagt
      121 tattgccatg aacgatattt cggggatggt ctatgatgaa aaaggcttgg atgttgattt
      181 agtagtggaa ttaaaagcta aacacttgaa tttagatgct tatgcaaaga tgcaccccca
      241 agttaaattc tcgcacaatt taaaggatat ctgggatatt cccggtgatt tagttttccc
      301 ctgtgccacc caaaatgaaa tcgggatgga ggaagctaag aagcttgttg ataacaaaac
      361 gcttttggtt tgtgagggcg ccaatatgcc cactaccaat gaagcgacac aatatttatt
      421 ggaacatggc gtcttgtttg cgccggggaa agcggccaat gccggtggtg tagccaccag
      481 cggtttggaa atgagtcaaa atgcaatgcg cttttcgtgg acatttgcag aagtcgatca
      541 acgtttaaaa gacattatga aaaatattta ccaaaatgtt tatcagactt cccttaaaat
      601 gggtcaaccg ggaaatctcg ttgttggcgc caatgttgcc ggttttctta aagtatatga
      661 agcaatgaaa gcacaaggag tgatttaaaa tggctaaaaa aatgaccgat gctaatctca
      721 aaagttattc acgcttagtg aaagaaccta aatatttgga ctatttaggg gaatttttaa
      781 ttgacagtga gcaaattgtt gattcgttca aatccgccaa agaaggtgtt gtgttcacca
      841 acaaacggat tattattatc agtgtttccg gagcttttgg aaaaaagcgc cagtttacct
      901 cctatcctta tcatcggatt actacttttg tagtgtcaac cgctaaggat ttagaaagca
      961 atgccgtcct ggatctaggt tatttcggaa cgccgaattt gcgttttgaa tttagtggta
     1021 aatctgaaat caaaacgatt atgaaataca ttaccgaagc tgtgataaaa taagagaaag
     1081 gattggattt aatgttatca gatattgaaa ttatcaatca agcacagctt gttgatatga
     1141 aaaggattgc gaggcgcaat ggcattaaag aagatgaact gtatttaatt ggcaagcatc
     1201 ttgcgaaagt ggaattttca gcttttgaac gtttaaaaga tcgcaaagac ggaaaa
//
LOCUS       DAOSGN010000339         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_115680_length_1256_cov_2.044130, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000339 DAOSGN010000000
VERSION     DAOSGN010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115680_length_1256_cov_2.044130"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1256
                     /locus_tag="PK087_04660"
     CDS             <1..>1256
                     /locus_tag="PK087_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18596.1"
                     /translation="VYVLNFTKQQSPEALVTSFVFNNQVVTLSNSETSYTSEIPYGIF
                     YRSTDSQTNLVNFSNLSSISNVYYNNLTGANLPSYLNALEISPYATLTSISLNVSMYD
                     TYRHRYAITYNIRAENGNTATFTHYLLEKEVNLNVVESFVDGNAVESPYNVVAFERED
                     SPTVRLNYNFANVYIPDSSYLTVAPSFSGSGTAVEDLHYFIDTFRTYGFEVDFSADAP
                     VGAYHFTMSFANSAQITPSINVSWNLTFQEVTITKKLNNNSHLSQINFVTDTVFTGLD
                     TVMDIALLTDVSYQGYLDDRTSREIIVLPTSGISYNDYLNYQEYWVIGQVQRTNLSYY
                     APTFTLPLGASIYRIIDEDNADDPLLQSTDFSTDFNPIGDGTTFNFVHYRVYAEDYDK
                     DNPTYNTHYTDYHVAVQDVTNNIRFE"
BASE COUNT          350 a          247 c          238 g          421 t
ORIGIN      
        1 gtctatgtcc tcaattttac caaacagcaa agcccggaag ctctcgtcac cagttttgtc
       61 ttcaacaatc aagtggtgac attatccaat tcagaaacat catatacttc ggaaatccct
      121 tatggtatct tttatcgtag cacggacagt caaactaatt tggtcaactt tagtaatttg
      181 tccagcattt ccaatgttta ttataataat ctaacgggtg ctaatttacc aagttattta
      241 aatgctttag agatttctcc ttatgcgact ttgacttcaa ttagtttaaa tgtcagcatg
      301 tatgatactt atcgtcaccg gtatgcgatt acctataata tcagggccga aaatggcaat
      361 acggccactt ttacccacta cttattggaa aaagaggtta atcttaatgt tgtggaatcg
      421 tttgtggatg gtaatgctgt ggagtcaccg tataatgttg ttgcctttga gcgggaagat
      481 tcaccaaccg ttcgcttaaa ctataatttt gctaatgttt atattcccga ttcttcctat
      541 ttaacggttg cacctagttt ttcaggctcg gggacggcgg ttgaagatct tcattacttt
      601 attgatactt tccgtaccta tggttttgaa gtcgatttca gtgctgatgc tccggtgggg
      661 gcatatcact tcaccatgag ttttgctaat tccgcccaaa ttactcctag catcaatgtt
      721 tcttggaact taactttcca agaggttacg attacgaaaa aactcaacaa caacagtcac
      781 ttatcacaaa tcaactttgt aacggatacg gtctttaccg gtttggacac ggtgatggat
      841 atcgccttgt taacggatgt aagctatcag ggttatttgg atgatcgaac ctcacgggaa
      901 attattgttt taccgaccag tggtattagt tataatgatt atttgaatta tcaagaatat
      961 tgggtgattg gtcaggttca aagaactaat ttaagttatt atgccccaac ctttaccctg
     1021 cctttagggg ccagtattta tcgcattatt gatgaagaca atgcggatga tcctttgctt
     1081 caatcgacgg attttagcac ggactttaat ccaatcggtg atgggacaac tttcaatttc
     1141 gtccattacc gggtttatgc ggaagattat gataaagata atccgactta taatacccat
     1201 tacaccgatt atcatgttgc tgttcaagac gtcaccaaca atattcgctt tgaaat
//
LOCUS       DAOSGN010000340         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_115797_length_1255_cov_2.291667, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000340 DAOSGN010000000
VERSION     DAOSGN010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115797_length_1255_cov_2.291667"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PK087_04665"
     CDS             complement(<1..757)
                     /locus_tag="PK087_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18597.1"
                     /translation="MENLIKQYENLRKKMKAYQFANYLASWDASTEAPKGCFANRSIQ
                     MGVLSEEAYKLQTSEETSNIVDELFAKKEQLEPILKHEIEETKRSLDNMKKIPMNEYV
                     EFMTILSDSQQIWAEAKKAADWSKFAPTLEKIVAYLKKYVQYLETDRLKGYDVLLNDF
                     ERDFLMEDCDLFFDALKRDLVPFVKKVLKKPLVYQNHFENATYPVEKQKQFIYYLQDV
                     MGFDRNRGLMKESEHPFTSGFGSSDVRFTVHYYE"
     gene            867..>1255
                     /locus_tag="PK087_04670"
     CDS             867..>1255
                     /locus_tag="PK087_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18598.1"
                     /translation="MKDIVFAFKSLGLLIKKYPITLLSEILITLSSLAMTLIPIYVVQ
                     TIVTDYQAGASISTILEKIGLYLVALIGLSLIQLLLSFVTIYVARNFRVTVATMLFQK
                     LKHIDYDFHENTTFLNNYTRSLEEGPDR"
BASE COUNT          359 a          234 c          221 g          441 t
ORIGIN      
        1 tttcataata gtggacggtg aagcgaacat cgctggaccc aaaacccgat gtaaacggat
       61 gctcactttc tttcatcaag ccgcggttgc ggtcaaaacc catcacatct tgtaaatagt
      121 atataaactg tttttgtttt tcgacggggt aggtcgcatt ttcaaaatgg ttttggtaaa
      181 cgaggggttt tttaagcact tttttcacaa acggcactaa atcgcgtttt aaggcatcaa
      241 agaataaatc acaatcttcc atcaagaagt cacgttcaaa atcgttcagt aaaacatcat
      301 aaccttttaa acggtcagtt tctagatact gcacatattt ttttaaatag gccacgattt
      361 tttccaaagt gggagcaaat ttagaccaat cggcagcctt tttggcttct gcccaaattt
      421 gttgactatc agataaaatt gtcatgaact ctacatattc attcatggga atttttttca
      481 tattatctag actgcgtttt gtttcctcaa tttcatgttt taaaatcggc tccaactgtt
      541 ctttcttggc aaacagttca tcaacaatgt tactggtttc ttctgaagtt tgtagtttat
      601 aagcttcttc gctcaaaacc cccatttgga tggagcggtt agcaaaacag cccttggggg
      661 cttcggtgct ggcatcccaa ctggcaagat aattggcaaa ttggtaggct ttcatctttt
      721 ttcgtaggtt ttcatattgc ttaattaagt tttccatatt ttcctccgct cattattata
      781 tcataattct tttgattgct ttaaaaaaac tgaaaattgg ttgtaaatga aagttataat
      841 cagtataata attaataggt gtgattatga aagatattgt ttttgccttt aaatcattgg
      901 gattgctcat aaaaaaatat ccgattactt tattaagcga aatcttgatt actttaagca
      961 gtttagcaat gaccttaatt ccgatttatg ttgtacaaac aatcgttacc gattatcagg
     1021 cgggtgcaag tatttccacc attttggaga aaatcgggct gtacttagtc gctttaattg
     1081 gcctttcgct gattcaatta cttctctcct tcgttactat ttatgtcgct cgcaactttc
     1141 gggtaacagt agcaacgatg ttgtttcaga aattaaagca tattgattat gattttcatg
     1201 aaaacaccac tttcttgaat aattatacgc ggtccttaga agaaggcccg gaccg
//
LOCUS       DAOSGN010000341         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_115930_length_1254_cov_2.342786, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000341 DAOSGN010000000
VERSION     DAOSGN010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115930_length_1254_cov_2.342786"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..769
                     /gene="deoD"
                     /locus_tag="PK087_04675"
     CDS             59..769
                     /gene="deoD"
                     /locus_tag="PK087_04675"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOH18599.1"
                     /translation="MATPHIEVNDSSLIAKTVLMPGDPLRAKFIADTYLKDVQLINSV
                     RNMLGYTGYYKGKKITVMGSGMGMPSIGIYSYELYQVYGVEQIIRVGSCGSYSPIINV
                     YDVIIVTDAYSESSYAKTAYNMDENTLPGDFDLIKDLNNSAKMLGYPVFNEIIHSSDV
                     FYRADSHEFKQIRDQFGCAAVEMESFALFANAIYLNKKAACILTVSDSLVTNQATTAQ
                     ERQVSFNKMMEIALEAAK"
     gene            807..>1254
                     /locus_tag="PK087_04680"
     CDS             807..>1254
                     /locus_tag="PK087_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18600.1"
                     /translation="MEIQKYVLNQVPVTLIKTSKFKTTVVEMMFLGSFSKANATKHSL
                     LLRLLRASTKRYPTKKALTNRLFELYDASVAIHSLPSFATNVTFVSMELINDQYASKD
                     SSLLEAGINLLAEILFNPNLDGSRWNQHDFLEQKRILKAHIENVYNQ"
BASE COUNT          392 a          224 c          237 g          401 t
ORIGIN      
        1 taaagcgggt gcaacccgga ttggcgcttc atcgggaaag gatttagtgt gaatatttat
       61 ggcaacacca catattgaag ttaatgattc tagtttaatc gctaaaacgg ttttaatgcc
      121 cggcgatccc ttgcgggcta aatttattgc tgatacttat ttaaaggatg tgcaattaat
      181 taattccgtt cgcaatatgc tcggctatac cggttattat aaaggcaaaa aaatcaccgt
      241 gatgggttcg gggatgggaa tgccgagcat cggcatttat tcctatgaac tttatcaagt
      301 ttatggtgtc gagcaaatca ttcgggttgg ttcttgtggg tcctattccc caattatcaa
      361 tgtctacgat gtcattattg ttactgatgc ttacagtgaa tcttcatatg cgaaaacggc
      421 ttataatatg gatgaaaaca cgcttcccgg tgattttgat ttaatcaaag atttaaataa
      481 tagtgctaaa atgctggggt atccggtttt taatgagatt attcattcat ccgatgtctt
      541 ttatcgtgcc gacagccacg aatttaaaca aattcgtgac caatttggct gtgctgccgt
      601 cgaaatggag agctttgctt tatttgccaa tgccatctat ttgaacaaaa aagcggcttg
      661 cattttaaca gtatcggatt ctttagtaac aaaccaagca acaactgccc aagagcgaca
      721 agtttctttt aacaaaatga tggaaattgc tcttgaagca gcaaaataga gtggttagcc
      781 actctttttt ttagaaaaga gataatatgg aaatacagaa atatgtctta aatcaagttc
      841 ccgtgacttt gattaaaact tctaaattta aaacaaccgt tgtagaaatg atgtttttag
      901 gatccttttc gaaagctaat gccaccaaac acagtttatt gttaaggttg cttagggctt
      961 ccacaaagcg atatccaact aaaaaagccc ttactaatcg tttgtttgaa ctatatgatg
     1021 ccagtgtggc catccattcc ttgccatcct ttgcaaccaa tgttaccttt gtatccatgg
     1081 aattgattaa tgatcaatat gcttcaaaag attcatcatt attagaagca ggaataaact
     1141 tgttagcgga gatcttattt aatccaaatc ttgatggttc tagatggaat caacatgact
     1201 ttttagaaca aaaacgcatt ttaaaagccc acattgaaaa tgtttataac caaa
//
LOCUS       DAOSGN010000342         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_116187_length_1252_cov_2.947368, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000342 DAOSGN010000000
VERSION     DAOSGN010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116187_length_1252_cov_2.947368"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..763)
                     /locus_tag="PK087_04685"
     CDS             complement(56..763)
                     /locus_tag="PK087_04685"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOH18601.1"
                     /translation="METFYLGGLGPKYKKIIIFDVETTGFNPIIDELLEIAMIVLTLD
                     DVGNVIKKEELDELIQLAPGKTIPDEIVQLTKITNDLLIQKGSNPLKVFKQVSSFMVG
                     QEELALLAYNASFDLQFLFAFFKKMKMEHILNLSHIDYYDCLTMYKDRAPYPHKLQDA
                     INFYGLKEEVKNSHRALDDARATLVLFQTMMNQCDDGLNYRNLFGYNPKYPRRHHPIH
                     KIKFRPQAYGASKKIYK"
     gene            complement(953..>1252)
                     /locus_tag="PK087_04690"
     CDS             complement(953..>1252)
                     /locus_tag="PK087_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HOH18602.1"
                     /translation="GLSFSGGEPFEQAKMFAELAKMAKALKLSIVTYTGYTYEEIIAR
                     KNPGWEELLSQTDLLIDGKYDISRKNLLLRFRGSENQRIIDLNKTMKEKTLVLWD"
BASE COUNT          387 a          217 c          205 g          443 t
ORIGIN      
        1 ccattgctta gaaagaatgc aagattgctt gttctcaata ttatgcaaag aaattttatt
       61 tatatatttt tttactggca ccataggctt gcggtcggaa ttttattttg tgaataggat
      121 gatgacgacg cggatatttg ggattatagc caaataaatt gcgataattt aagccatcat
      181 cacattgatt catcatcgtt tggaaaagga cgagggtcgc tctagcatca tcaagggcac
      241 ggtgggaatt ttttacttcc tcttttaatc cataaaagtt aatggcatcc tgtaatttat
      301 gggggtaggg ggcacggtct ttatacatcg ttaagcaatc ataataatca atgtgggaaa
      361 ggtttaggat gtgttccatc ttcattttct taaagaaagc gaacaaaaac tgtaaatcaa
      421 aactggcatt ataggcgagg agggccagtt cttcttgacc aaccataaag gaactgactt
      481 gcttgaaaac tttaagagga ttgcttcctt tttgtataag caaatcattc gtgattttag
      541 ttaattgaac aatttcatca gggatggttt ttcccggtgc tagttgaata agctcatcta
      601 attcttcttt ttttataaca ttaccgacat catccaaagt taggacaatc atagcaattt
      661 caagtagttc atcaataata ggattaaatc ccgttgtttc cacatcaaag atgattattt
      721 ttttgtattt agggcctaaa ccaccaagat aaaaagtttc catggtgttt tctccttcat
      781 agcttttata gtttctttgg ttttctcttc atggataatt aaagccaata aagagtttcc
      841 tttttgttat aaattttgct aaaaacacat agaaaggaaa ctcttcattt catgataaca
      901 caaaacatct aaaattggca ttaatatttt aaatatcatc acctttttat tattaatccc
      961 aaagaacaag agttttttcc ttcatcgtct tatttaaatc aataattctt tgattttctg
     1021 aaccacgaaa tcgtaaaagc aaatttttcc tactgatatc atactttcca tcaattaaaa
     1081 gatcggtttg ggaaagcaat tcttcccatc cgggattctt tcttgctatg atttcttcat
     1141 aagtatatcc tgtgtaggta acaatactaa gttttaatgc tttggccatc tttgctaatt
     1201 ccgcaaacat cttggcttgt tcaaagggtt caccaccgct aaagcttaaa cc
//
LOCUS       DAOSGN010000343         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_116943_length_1247_cov_2.180369, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000343 DAOSGN010000000
VERSION     DAOSGN010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116943_length_1247_cov_2.180369"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..708
                     /locus_tag="PK087_04695"
     CDS             19..708
                     /locus_tag="PK087_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18603.1"
                     /translation="MAITKLFNYFKTGFDEFNNFVIANALYETDRRLLRDEFISDWNQ
                     VFATNWTELDALSFFNSAKLGTTSSTDMANSNLYRFFNNSTMKKKWAWLLDYLDSYAM
                     DNIEITNQIEAMQGNGTNNGKNLFNASHLIYSITNFFNKTNETGSYSALDFTVDAYEK
                     YQTIALYNNAVYTNYLHYQFVQKGSSIMIPKGAEIEGYRFVGYYGAAVAGANYTVTDT
                     IAFVPRYILND"
     gene            920..1192
                     /locus_tag="PK087_04700"
     CDS             920..1192
                     /locus_tag="PK087_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HOH18604.1"
                     /translation="MKTSVFKALSDPIRRHILLELKKGRLNAGELAEMFQISPQALSY
                     HLRLLKEADLVMEYKVKNYIYYEWNATVFDEIYLWFKQMRGGDNEK"
BASE COUNT          421 a          180 c          229 g          417 t
ORIGIN      
        1 tccgtttcgt aaagagcgtt ggcgataaca aaattattta attatttcaa aaccggtttt
       61 gacgaattta ataattttgt tatcgccaac gctctttacg aaacggatcg aagattgttg
      121 agagatgagt ttataagtga ttggaatcaa gtttttgcta ctaattggac agaacttgat
      181 gctctaagtt tctttaacag tgccaaattg ggaacaacga gttcaacgga tatggccaat
      241 agtaatttat atcgtttctt taacaattca acgatgaaga aaaaatgggc ttggttattg
      301 gattatttag atagttatgc catggacaac atcgagatta caaaccaaat tgaagcaatg
      361 caaggaaacg gaaccaacaa tggtaaaaac ttgtttaatg catcacattt gatttattca
      421 atcactaatt tctttaataa aactaatgaa acaggctcat attcggcact tgacttcact
      481 gttgatgctt atgaaaagta tcaaacgatt gccttgtata acaatgctgt gtacacaaat
      541 tatcttcatt atcaatttgt acaaaaaggc agtagtataa tgattccgaa aggagccgaa
      601 atcgaaggtt accgctttgt tgggtattat ggagctgctg tggcgggggc caattataca
      661 gtcaccgaca cgattgcttt cgtaccgcgt tatatcctta atgattaaaa aaagcgcttc
      721 atacatgaaa atgtgtatgg agcgtttttt atcgtactgt aaaaatggat attctttctt
      781 tttaggaggt acataagtat ctcttttttt aaaaaaagaa ctttaaaaaa aactatttaa
      841 aaataatttt aaatagttgt tgacaatctc acttatttat attaaaatct atttaaagat
      901 attttgaaat aggtataata tgaaaacaag tgtatttaaa gctttaagtg accccatccg
      961 tcgtcatatt ttgctagaac ttaaaaaagg ccgtcttaat gccggtgaat tggcggaaat
     1021 gtttcaaatt agtccccaag cactgtccta ccatttaaga ctattgaaag aagccgattt
     1081 agtcatggaa tataaagtca agaattatat ctattatgag tggaatgcga cggtttttga
     1141 tgagatttat ttatggttta aacaaatgcg aggtggagat aatgaaaaat aaggttttgt
     1201 tattattggc agttttaccc tttttgatca caatcttgtt gttacgg
//
LOCUS       DAOSGN010000344         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_117571_length_1243_cov_2.315657, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000344 DAOSGN010000000
VERSION     DAOSGN010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117571_length_1243_cov_2.315657"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PK087_04705"
     CDS             complement(<1..257)
                     /locus_tag="PK087_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOH18605.1"
                     /translation="MDLQVKDGWIEVISGSMYAGKTEELIRRVKRIEYARKEVIVFKP
                     LIDNRYSSNEVVSHDNGRTKSIDISNPEEIYQHLNGRLPYAV"
     gene            472..690
                     /gene="rpmE"
                     /locus_tag="PK087_04710"
     CDS             472..690
                     /gene="rpmE"
                     /locus_tag="PK087_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOH18606.1"
                     /translation="MKKGIHPKYQNVTVVCTTCGNTFETGSVLKEMRVDTCSKCHPFY
                     TGQQKFVQAAGRVERFNKRLNQKQSANK"
     gene            complement(803..>1243)
                     /locus_tag="PK087_04715"
     CDS             complement(803..>1243)
                     /locus_tag="PK087_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOH18607.1"
                     /translation="QLKRRFDLIPNLVETAKGYANLEKGIFEEFAKARGLYAQAAQAG
                     NVEGMAQANQSLGGTLSRLLMVQEQYPELKANTNFQDLMAQLKDTEDKISFNRQFYND
                     TVLGYNNKIELFPSNIIANIFRFQAAQFFEVKDEAQREAPKVKF"
BASE COUNT          396 a          230 c          198 g          419 t
ORIGIN      
        1 accgcataag gcaaacgacc gtttaaatgt tgatatattt cttcgggatt actgatatca
       61 atggatttcg tgcggccgtt atcatgtgac accacttcat tactgctata gcgattatca
      121 attaagggtt taaaaacaat cacttcttta cgagcatatt caattcgttt gactctccga
      181 atcagttctt cggtttttcc cgcatacatg cttcccgaaa taacttcaat ccatccatcc
      241 tttacttgta aatccatctt tttcacctct aaaatattat accttattaa aagtagtttt
      301 taaagttttt ttgtgttttc atgataatat ttttgataaa tcattataaa agtaagcaaa
      361 caaacacggg caaattaatg ttgtttttgt tgacttccac aagcttttat aatataatat
      421 acaaggtaaa cttactatag gtttaaaaac ctatggcgga aggagataat gatgaaaaaa
      481 ggaattcacc ccaaatacca aaatgtaacc gtcgtttgta caacttgtgg aaacactttt
      541 gaaactggtt cggttttaaa agaaatgcgt gttgacactt gttcaaagtg ccaccccttt
      601 tataccggcc aacaaaagtt cgttcaagcg gccggtcgtg ttgaaagatt caataaacga
      661 ctcaatcaaa aacaaagcgc aaacaaataa gtttgcgttt ttttattgct cgcttgattt
      721 aattgtaaaa atgttaacaa tcttagtatt ttaaaaaagc ttttttgaaa agaaatcaaa
      781 aaagcttatt aacaatattt gtttaaaatt taactttcgg agcttcccgt tgagcttcat
      841 ctttaacttc aaagaattga gctgcttgga aacggaagat attagcaata atattgcttg
      901 ggaataattc aatcttgtta ttgtagccca aaacggtgtc attgtaaaac tgacggttga
      961 aagaaatttt atcttccgta tcttttaatt gagccattaa atcctggaag ttggtattag
     1021 cctttaattc cggatattgt tcttgcacca ttaataaacg gctcagcgtg ccacccaagg
     1081 attggttagc ttgcgccatt ccttccacat tcccagcttg agcagcttga gcatataaac
     1141 ctctagcctt tgcaaattct tcaaaaatgc ctttttccaa attagcatag cccttggctg
     1201 tttccactaa attagggatt aaatcaaaac gacgttttag ttg
//
LOCUS       DAOSGN010000345         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_118387_length_1237_cov_2.954315, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000345 DAOSGN010000000
VERSION     DAOSGN010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118387_length_1237_cov_2.954315"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1051
                     /gene="rsmH"
                     /locus_tag="PK087_04720"
     CDS             122..1051
                     /gene="rsmH"
                     /locus_tag="PK087_04720"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOH18608.1"
                     /translation="MQKHIPVLLKETIEGLNIKENGIYVDCTMGGAGHSAEILRRLSK
                     NGHLYCFDQDDYAIYRGQARLLEIGPNFTVIPDNFVNLKENLKKIDISQVDGVLYDLG
                     VSSFQFDVGERGFSYNFDSRLDMRMNQKQDLSAFDIVNYYSYDELKKIFYLYGEERFA
                     PGIAKRIVKEREKQPIETTFELVEIVKKSIPAFARRTGGHPAKQVFQALRIAVNDELN
                     VFEKSLSDAISLLKSQGRIAVITFHSLEDRICKNVFKKHVENQFPSKLPLRANEIKPK
                     FRLVNNKVITPTENELQSNHRAHSAKLRILEKL"
BASE COUNT          431 a          188 c          241 g          377 t
ORIGIN      
        1 tcatcggtgt tggctcccga attgaaattt gggataaagc cgaatggcag aagtattact
       61 tagatcgcca gtcagttatt gacacaatta gcgaagaaat cgagcttgat atttaatgga
      121 aatccaaaag cacattccgg ttttactaaa ggaaaccatt gaaggactaa atatcaaaga
      181 aaatggaata tatgttgatt gcacaatggg cggggcaggc cattcagcag aaattttaag
      241 gagactatca aaaaatggac atctatattg ctttgatcag gatgattatg ctatttatcg
      301 cggccaagct cggcttttgg aaataggccc caatttcacc gttattcccg ataactttgt
      361 caatttaaag gaaaatttaa aaaaaattga tattagtcaa gtggatggcg ttttatatga
      421 tttaggagtc tcttctttcc aatttgatgt tggtgaaagg ggcttcagtt ataattttga
      481 ttcacgccta gacatgcgca tgaatcaaaa acaagattta tcagcttttg atattgtgaa
      541 ttattatagt tatgatgagc ttaaaaagat tttttatctt tatggagaag agcgatttgc
      601 acctggaatc gctaaaagaa tagttaaaga acgagaaaaa cagccgattg aaacaacttt
      661 tgaacttgtt gaaatagtta aaaagagtat tcccgcattt gctagaagaa cgggtggtca
      721 tcccgctaaa caggtttttc aagctttaag gattgcggtt aatgacgaat taaatgtttt
      781 tgaaaaaagt ctttccgatg cgatttcatt attaaaatca cagggaagga ttgccgtaat
      841 cacctttcat tctttagaag atcgtatttg taaaaatgtt tttaagaaac atgttgaaaa
      901 tcagttccca agtaaacttc cacttcgagc taatgaaatt aagccaaagt ttcggcttgt
      961 taacaataaa gtaattacgc caacggaaaa cgaattacaa tcaaatcatc gtgcccatag
     1021 tgccaaatta agaattttag aaaaattata ggaggtatat catgagcaac tttcgacctt
     1081 taccaaaacc agtttgccca cgtccaacga agtaatgaac aatgtgaaga aaacacatgt
     1141 caaggtagga actttttccc tgctggtgca tgtgtttttt gtttggtaga ttgtaaatag
     1201 aaagtagaaa aaaaatttat gataaacata tttgtaa
//
LOCUS       DAOSGN010000346         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_118397_length_1237_cov_2.781726, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000346 DAOSGN010000000
VERSION     DAOSGN010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118397_length_1237_cov_2.781726"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1237)
                     /locus_tag="PK087_04725"
     CDS             complement(<1..>1237)
                     /locus_tag="PK087_04725"
                     /inference="COORDINATES: protein motif:HMM:NF039260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HOH18609.1"
                     /translation="GLIHAQLPYQGVKIKINPDQQERDEVLKQASHYQQVVIFTYNAF
                     SNPEQALLVKQLNQVVPQLFVVSTRNPYDILALSGVANYYCLYEYTPNSVKTAMRFLK
                     GDLLATGQLPVSLKPRMKIGASVYVGLEQPLSANLAYLDVLKAHNIELLFISAHMPEM
                     RPDFTDELREIVTKANHLGFKIILDVSKPMLEDFVIPDIYSLRLDYGFSLEEMLELAN
                     KYPFFLELNASTVSEQQLKTLLMMGLDLRRLRVSHNFYPKPYTGLTQEQVLKQNLMFK
                     RYGLTVMMYIPSQNEKRGPVYEGLPSVEDHRYLPLEAVLSEMAFLQTDEVVFGDSFAS
                     SQELQTAINFSFSQIVIPIALSTAISQEEQTQLLLSHRERADANAFGIRSSTRLKSGL
                     ILPNHTVERKVLAVTIDNH"
BASE COUNT          392 a          235 c          258 g          352 t
ORIGIN      
        1 aaatgattat caattgtgac cgccaacact ttccgttcaa cggtatgatt cggaagaatg
       61 agaccgcttt ttagtcttgt ggaagaacgg ataccaaagg cgttggcatc ggcacgttca
      121 cgatgggaaa gcaacagctg ggtttgttct tcttgggaaa tggccgtgct tagggcaatc
      181 ggtattacaa tttgggaaaa ggaaaaattg atggccgttt gtaattcttg agaactggca
      241 aaactatcac caaagacaac ttcatcggtt tgcaagaacg ccatttccga taaaacagct
      301 tctaaaggca aatagcgatg gtcttcaacg ctcggcaaac cctcataaac cggaccacgt
      361 ttttcgtttt gggacggaat atacatcatc actgttaagc catagcgttt aaacataagg
      421 ttttgtttta agacctgttc ttgagttaaa ccggtatagg gtttcggata aaaattatgg
      481 ctgacgcgta agcgtcgcaa atctaatccc atcattaaga gggtcttaag ttgttgttcg
      541 cttaccgttg aagcattcag ttctaaaaag aacggatatt tattagccag ttcaagcatt
      601 tcttccagtg aaaatccata atccaaacgc agtgaataaa tatcaggaat aacaaaatct
      661 tcaagcatcg gtttagaaac atccaaaatg attttaaaac cgaggtgatt ggccttcgta
      721 acaatttccc ttaattcatc agtaaaatca ggccgcattt ccggcatatg agcggaaata
      781 aacaggagtt caatattatg agcttttaaa acatccaaat aagcaaggtt agcgcttaag
      841 ggttgttcta aaccgacata aaccgacgct ccgattttca tccttggttt aagcgaaacc
      901 ggtaattgac cggtggctaa taaatcacct tttaaaaagc gcattgccgt cttaacggaa
      961 ttaggcgtat attcatataa acaataataa ttggcaacgc cactaagagc caagatatca
     1021 tagggattac gtgtggaaac aacaaagagt tggggcacaa cttggtttaa ttgtttaaca
     1081 agcagcgctt gctcaggatt actaaaagca ttataggtaa agatgacgac ttgttgataa
     1141 tgagaagcct gttttaacac ttcatctctt tcttgttggt cgggattgat tttaatcttc
     1201 acaccttgat agggaagttg agcatgtatt agcccat
//
LOCUS       DAOSGN010000347         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_119347_length_1231_cov_3.198980, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000347 DAOSGN010000000
VERSION     DAOSGN010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119347_length_1231_cov_3.198980"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1231
                     /locus_tag="PK087_04730"
     CDS             <1..>1231
                     /locus_tag="PK087_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH18610.1"
                     /translation="RGVAKGSAVITVSLSKYPNIKATVNITVTEEDQPIVEPSVPNMM
                     GQDFIIMVNKASTTDPRSEAYVGNWKKEKVALIAEVEKKYNVKVVYKTYPADAVWGGG
                     RERFITTNSVNNTPQAHIYEMPSYSIAVLAEGGAVLPLDALIEKYGNQGYWPEKAAFG
                     TVLGKQYAYDDAYPLVGQGIFYNIDLLETYLGEGKGTLPSDLWMAGEWDWNAFKNICN
                     QLLPGIAGLNVEGAAVIGGRPYNWAYFMLGANGTHVVSSDLKSELATQAGIDTLTYLN
                     SLISIPGMWEFESAALSNATCPQFKGGNIAFHNGEPYWLYDATKWLGYTFRMGFVPYP
                     VGPNVKSDLSNYYINEVYGKTSYLVSSSYSLELVPPGYENIAFHEESIFRIWAYLQYF
                     PDIDPKTGKYCKSAQILK"
BASE COUNT          345 a          233 c          297 g          356 t
ORIGIN      
        1 aggggggtag caaaaggcag tgcggtaatc acggtttcgc taagtaaata ccccaatatt
       61 aaagcgaccg ttaatattac ggtgacggaa gaagaccaac cgattgttga accatcggtt
      121 cccaatatga tgggccaaga tttcattatc atggttaata aagcctctac taccgaccca
      181 cgtagtgaag cttatgttgg taattggaaa aaggaaaaag tcgccttaat tgcggaggtt
      241 gagaaaaaat ataatgtgaa agtggtttat aagacctatc ccgccgatgc tgtatggggt
      301 ggtggtcggg aacgcttcat taccaccaac tcggttaata ataccccgca agcccatatt
      361 tatgaaatgc cgtcctattc cattgccgtt ctggcggaag gtggggccgt tttaccgctc
      421 gatgctttaa ttgaaaaata cgggaatcaa ggatattggc cggaaaaagc ggctttcggc
      481 accgtcttag gtaaacaata tgcctatgat gatgcttatc ctttagttgg tcaaggcatt
      541 ttctacaaca ttgatttatt agaaacttat ttgggtgaag gtaaaggaac tttgccgagc
      601 gacttatgga tggctggtga gtgggattgg aatgctttca aaaacatttg caatcaactg
      661 ttaccgggga tcgctggact taatgttgaa ggggcagctg ttattggcgg acgtccttac
      721 aactgggctt attttatgct cggggctaac gggacccatg tagtttcttc ggatttgaaa
      781 tcggaattag cgacgcaagc gggaattgat acattaacat atttaaatag cttaatcagc
      841 attccgggaa tgtgggaatt tgaaagcgcc gctttatcga atgccacttg cccgcaattt
      901 aagggtggga acatcgcttt ccataatggc gaaccttatt ggttatatga tgcgaccaaa
      961 tggttgggct atactttccg gatgggcttt gttccgtatc cggtgggacc gaatgttaag
     1021 agtgatttaa gcaattatta catcaatgaa gtttacggta aaaccagtta tttagtttcg
     1081 tcttcctatt ccttggaact ggttcctccc ggttatgaaa acattgcttt ccatgaagaa
     1141 tctattttta gaatttgggc ctatttgcaa tacttcccgg atatcgatcc gaaaaccggg
     1201 aagtattgca aatcggccca aattctaaaa a
//
LOCUS       DAOSGN010000348         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_119681_length_1229_cov_2.749574, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000348 DAOSGN010000000
VERSION     DAOSGN010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119681_length_1229_cov_2.749574"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PK087_04735"
     CDS             <1..678
                     /locus_tag="PK087_04735"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH18611.1"
                     /translation="TLEAYLKSQGKTFVRNLFFNPINLEWLVPGYDHHEHPGAPAIPY
                     NTPYYFLMMANGYNTNGQLDAYHLDLKKFRLSEDVKSRIAKNEADGYTITYYDPKLHY
                     GFDELFDALKNEGWREVVKNNLAKAKPDPMLIVQKEGEILGWTGPVITQASGRGYLGG
                     IGVHPKTQGHGLGKSLFCMLCQCSKDNGATFMTLFTGADNKAREIYLYAGMRIVQSFA
                     VLRKELH"
     gene            678..>1229
                     /locus_tag="PK087_04740"
     CDS             678..>1229
                     /locus_tag="PK087_04740"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOH18612.1"
                     /translation="MKTLMSIGAHVGDAELSTGCVLAHQSLKGDKIITVALTAGERGN
                     PKHLSVADYRKQKVKEAQEFAKMLKGEAIVLDYPDGELPDNDEVRVQVANLIRQYRPH
                     YLFTHWEKSMHKDHALTHKIVVEAAFWAALDMGDKITGTPHWAPVYFSENWEDREGFE
                     PYLFVNCTDGYELWKEAISQHWFI"
BASE COUNT          345 a          240 c          298 g          346 t
ORIGIN      
        1 actttggaag cttaccttaa gtcccaaggg aaaacctttg ttcgcaatct ctttttcaat
       61 ccgattaatt tagaatggtt ggttccgggt tatgatcacc atgaacatcc gggagctccg
      121 gccattccct ataatacccc ttattatttc ttaatgatgg ccaatggtta taataccaac
      181 gggcagcttg atgcttacca tttagattta aagaagtttc gcttatcgga ggatgtgaaa
      241 agccgaatag ctaaaaatga agccgatggc tatacgatta cttattatga ccccaagctt
      301 cattatggct ttgatgaact gttcgatgcc cttaaaaacg agggttggcg agaagttgtc
      361 aagaacaatc tagccaaagc taaacctgac ccgatgttga ttgtacaaaa ggagggtgaa
      421 attctgggct ggacgggacc ggttattaca caagcaagcg gtcgtggtta tttagggggg
      481 attggcgttc atccgaaaac ccaaggtcat ggcttgggta aatccttatt ttgcatgctg
      541 tgccaatgtt caaaagacaa cggtgcgacc tttatgacgc tcttcacggg tgcggataat
      601 aaagctcgtg aaatttatct ttatgccggg atgaggattg tccaatcatt tgccgtgtta
      661 agaaaggagt tacattgatg aaaaccttga tgtcaattgg tgcccatgtt ggggatgcgg
      721 aattaagcac gggttgtgtt ctagcccatc aaagtttaaa aggcgataaa atcattacgg
      781 tcgctttaac ggccggagaa cgcggtaatc ccaagcatct aagcgtggct gattaccgga
      841 agcaaaaagt aaaagaagcg caggaatttg ccaagatgtt aaagggggaa gccattgtcc
      901 ttgactatcc cgatggtgaa ctacccgata atgatgaagt tagagtgcaa gtcgccaatt
      961 taattcgcca atatcgcccc cattaccttt ttacgcattg ggaaaaatcc atgcataaag
     1021 accatgcttt gactcacaaa attgttgttg aggccgcttt ttgggcggct ttggatatgg
     1081 gtgataagat tacgggaacg ccgcactggg ctccggtcta tttttcggaa aattgggaag
     1141 accgtgaagg ttttgaaccc tatctttttg ttaactgtac ggatggttat gaactgtgga
     1201 aagaagccat ttcccaacac tggtttatt
//
LOCUS       DAOSGN010000349         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_120547_length_1224_cov_1.374679, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000349 DAOSGN010000000
VERSION     DAOSGN010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120547_length_1224_cov_1.374679"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PK087_04745"
     CDS             <1..872
                     /locus_tag="PK087_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOH18613.1"
                     /translation="GPLAQVSGALVLAASQLYELTKDEKYLDVAVLSAQEVMDCQEVN
                     LPNWQKPLRGFFYEDSDHKHYLSYEHRGHEQSPITGIIKLMAVAPHHPQFSKWKQTVT
                     LYQEYIEKTKDFILPYGLLPAHIYEKDKINLDRFTLPNRDLLDSYLRQLHEQIETGIA
                     LDDEVFLRRFPIAIQRRGFHATLLSKTKAVSAISQSLNSARMRQVAINQVEWILGKNP
                     FATSTMYGEGYNYHPLYVAFSQQLLGALPVGIKTKGSADVPYWPLVNNAVYKEVWGHT
                     TAKFLWVLADLLL"
     gene            937..>1224
                     /locus_tag="PK087_04750"
     CDS             937..>1224
                     /locus_tag="PK087_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18614.1"
                     /translation="MMGNYWLPYLLQTNIYSIIILVIILISLRQYKESLNLGNNIFKT
                     IIIVDIILMALDIFSCLIEGRPGAWVTGFYYATQVISLVIAPSIGVLWFYFI"
BASE COUNT          352 a          209 c          263 g          400 t
ORIGIN      
        1 ggggacccct tgcccaagtc agtggagcct tagttttggc ggccagccaa ttatatgaat
       61 taactaaaga tgaaaaatat ttggatgtag cggttttgtc cgctcaagaa gttatggatt
      121 gtcaggaggt caatttacca aattggcaaa agcccttacg tggtttcttt tatgaagata
      181 gtgatcataa acattattta agctatgagc atcgcggtca tgaacaatca ccgattacag
      241 gaattattaa attgatggcg gtagctcctc atcatcccca atttagtaag tggaagcaaa
      301 cggtgacttt ataccaagaa tatattgaaa agacgaaaga ctttatttta ccctatggtt
      361 tgttgcccgc ccacatttat gaaaaagata aaatcaatct tgaccgtttt acgctgccta
      421 atcgcgatct tcttgattct tatcttagac agttacatga gcaaattgaa accggaattg
      481 ccttggatga tgaagtcttc ttaagaagat ttccgattgc cattcaaaga cgaggttttc
      541 atgccacctt attgagtaag acgaaggcag tcagtgccat tagccaaagt ttgaatagtg
      601 caaggatgcg acaagtggca attaaccaag tggagtggat tttaggaaaa aatccctttg
      661 cgacttcaac gatgtatggc gaaggttata actaccatcc tttatatgtc gctttttcgc
      721 agcaactctt gggagcccta ccggttggca ttaaaacgaa gggttccgcc gatgttccgt
      781 attggccgct tgtcaataat gcggtttaca aagaggtgtg gggacacaca acggctaaat
      841 ttttgtgggt tttagcagat ttattgttgt aattattact tcacttttaa aaaatgtgat
      901 ataatgatgg taattcttag atgaaagcca ggtgatatga tggggaatta ttggctacct
      961 tatctacttc aaaccaatat ttatagtatc atcattttag tgattatctt aataagtctt
     1021 agacagtata aggaatcgct taatttggga aataatattt tcaagaccat cattatcgtg
     1081 gatatcattt tgatggcctt ggatattttc agttgcttga ttgaaggacg accgggggct
     1141 tgggttactg gtttttatta tgctactcaa gtcattagtc ttgtgattgc tccttcaatt
     1201 ggggttttgt ggttctattt catt
//
LOCUS       DAOSGN010000350         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_121016_length_1220_cov_3.048927, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000350 DAOSGN010000000
VERSION     DAOSGN010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121016_length_1220_cov_3.048927"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /gene="metG"
                     /locus_tag="PK087_04755"
     CDS             <1..998
                     /gene="metG"
                     /locus_tag="PK087_04755"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOH18615.1"
                     /translation="IYPHEIIDRYGLDAFRLFMTKEMPLGNDMVFTYERFFEKYNVDL
                     ANDLGNLVSRTIAMANKYFNGQVEKPIIHPLDVELELEQLAQEVFENYQSAFSGFRFQ
                     SGLMEVWNLINRTNKLIDETLPWDLAKDPLKKDVLNSVLYHLLESIRVIALMLLPVIP
                     DTVKIIFHELGLEESDFSTLSFGLTNTFQVVKKARVLFKRLDIEEELKFQASKQTPIR
                     KTSPIKAEITIDEFNKVDLRVGVVVDAKHLEGSNNLLVLQVQVEDKIRQIVSGIAQDY
                     NPSQLIGKKIVVVANLKPAKVRGAISEGMLLAASGDGLPFEVVELQNHDSYSKVS"
     gene            1000..1185
                     /locus_tag="PK087_04760"
     CDS             1000..1185
                     /locus_tag="PK087_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18616.1"
                     /translation="MFLSTTFSTEFFIPIIILLGIATIFTFLIKVLNLKFLPVFALFK
                     TKLGECLKKTAKESIIY"
BASE COUNT          369 a          187 c          243 g          421 t
ORIGIN      
        1 ttatttatcc gcatgaaata attgatcgtt atggcttgga tgcttttcgt ttgtttatga
       61 ccaaagaaat gcccttgggt aatgatatgg tttttactta tgagcgtttt tttgaaaaat
      121 ataatgttga tttagccaat gatttgggaa atttagtcag tcgaacgatt gccatggcta
      181 ataaatattt taatgggcaa gttgaaaaac cgattattca tcccttggat gttgaacttg
      241 agttggaaca attagctcag gaagtgtttg aaaattacca aagtgccttt tccggctttc
      301 gttttcagag cggcttgatg gaagtgtgga atttaattaa tcgcaccaat aaattgattg
      361 atgaaacttt accgtgggat ttagccaaag atcccctcaa aaaagatgtt ctaaattctg
      421 ttctttatca tctcttagaa agtattcgag tgatagcctt aatgttacta cccgttatcc
      481 ctgataccgt caaaatcatt ttccatgaat taggtttaga ggaatccgat ttttctacat
      541 taagttttgg tttaacgaat acctttcaag ttgtaaaaaa ggcaagggta ttatttaagc
      601 gcttggatat tgaagaagag ttaaaattcc aagcctctaa acaaacccct atacggaaaa
      661 caagccccat caaagcagaa atcacgattg atgaatttaa taaagtcgat ttgcgtgttg
      721 gagttgttgt tgatgctaaa catttggagg gatcgaataa tctattggtt ttacaagtgc
      781 aagtggaaga taaaatccgg caaattgttt ccggaatagc ccaagactac aatccaagcc
      841 aacttattgg taaaaaaatt gttgttgttg ccaatttaaa accggctaaa gttcgcggtg
      901 ccatctcgga aggaatgttg ttggctgcca gtggtgatgg acttccattt gaggttgtgg
      961 aattgcaaaa ccacgattcg tattctaagg tgagctaagt tgtttttaag tacgacattt
     1021 tccacggagt tttttattcc catcatcatc ttattaggga tagcgactat ctttactttc
     1081 ttaattaagg tgttaaattt aaagttttta cctgttttcg ccttatttaa aacaaaatta
     1141 ggagaatgtt tgaaaaaaac agcgaaggaa tcgattattt actgatacgg attatttttg
     1201 ttttttgttg tccatggttt
//
LOCUS       DAOSGN010000351         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_123469_length_1204_cov_2.286336, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000351 DAOSGN010000000
VERSION     DAOSGN010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123469_length_1204_cov_2.286336"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PK087_04765"
     CDS             complement(<1..381)
                     /locus_tag="PK087_04765"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOH18617.1"
                     /translation="MSYKNVVYGKNRVRRNYSRVQTNVELPDLIEVQTKSFSWFITTG
                     IKELFKELSPIRDHGEKLELYFEGFEFDEPKYTVSQAKRNDVNYSRPLKVDVRLVNKE
                     TGDIKEQKIFLGEIPMMTPWGTFII"
     gene            complement(482..1051)
                     /locus_tag="PK087_04770"
                     /pseudo
     CDS             complement(482..1051)
                     /locus_tag="PK087_04770"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT          380 a          247 c          178 g          399 t
ORIGIN      
        1 aataataaat gttccccaag gtgtcatcat cggaatttca ccgaggaaaa tcttttgttc
       61 tttgatatca cccgtctctt tgttaacaag gcgaacatca acttttaagg gtcgtgagta
      121 attaacgtca ttcctttttg cttgagaaac ggtatatttt ggttcatcaa attcgaaacc
      181 ttcaaaatac aattcgagtt tttcgccatg gtcgcgaatt ggagataatt ctttaaacaa
      241 ttctttgatg ccggttgtaa taaaccagct aaatgatttg gtttggacct caattaaatc
      301 cggtagctca acattagttt gcactcgcga ataatttctt cgcacacgat tttttccata
      361 aaccacattt ttatagctca aaatattcca cctcactaaa atttaagcaa aaaaggcgaa
      421 tgtgaaagaa atttccacat tcttccgcat tctattatgt tatcatattc ctgtcaaatt
      481 gtcaagttgt tttttttggc attttttcgc tctctaaaac caaatatccg ctttttttat
      541 taataatttc ggcattcaaa aagacagttt ccattttctc tttcaaggac aaagcacctt
      601 gttttttttg gatgacaaac aataattttc ccccgtcatg aagtttatga taagcctggt
      661 caacaatcgc atgaaccact tttttaccag cgcgaatcgg cggattggaa ataatcatat
      721 caaaggaatt caatacattc gctaaaagat tactttccat aatagtcaca ttaacgatgc
      781 cattttgttt agcattaatt tgcgaaagct caacggcccg agtcacgaca tccaccattg
      841 taaccactaa atccggatgc gttttcgcaa tcgacaaacc gataacacca tatccacagc
      901 cgacatccaa aatgtttttt taattatcaa gacgctcttt aatcgtttcc agcaagacat
      961 gggtcccaaa atcaatttgt tgacgactaa aaacacccgc atctgttaaa aatgtgagtt
     1021 gttctccttg atactgataa ttaattttca tcagtttttc ttttaaatta ggatcattaa
     1081 gataataatg actcataact tgtcctttcc caagaaaaaa gatctcatgc agcgagatct
     1141 tttccttttc ttaattggat aattaagaga gtaatcccag ctaaaaacat cacggcggaa
     1201 agaa
//
LOCUS       DAOSGN010000352         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_124084_length_1200_cov_2.783406, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000352 DAOSGN010000000
VERSION     DAOSGN010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124084_length_1200_cov_2.783406"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..>1200
                     /locus_tag="PK087_04775"
     CDS             105..>1200
                     /locus_tag="PK087_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH18618.1"
                     /translation="MHDEIINLIKTTTDLNSLRIQLNNYHQRDLAESFRLLDEDQQEK
                     VYALFSNEELADFFSYLDAEDVSEITETISEEKLASIIQEMEPDDAADLLAEYSDDRV
                     EMIYQFIDEDTKEEISELLEYEENTAGALMNSNYLVIEQGKDIKDLMKKLVKEAPDLE
                     SINTTFVVDANNQLLGTIPLKKVIMAKAPCPVESIMNPNFKAVNVDTPIEDAVNIVKN
                     YDIFDLPVLESGQLKGIITIDDAMDTVVEEAEEDYAKLAGLTEAEEITESTFTSVKKR
                     IPWLAFLLIMDLFVAVVISQYDYLFALDKMTVFVFFQPIILGLAGNFGTQSLAITIRK
                     ITDHEFDYRHRILKHLIKETALGLAMGLLLG"
BASE COUNT          387 a          218 c          223 g          372 t
ORIGIN      
        1 ctctggacca tttatcacca ctgtcaatga tgttttttcg acccttgtat actttggttt
       61 agcttattta ctcttcagtt atctttagaa aggagactta ggtcatgcat gatgagatta
      121 ttaatctaat aaaaaccaca actgatttaa acagtttacg aatccaacta aataattatc
      181 atcagcgtga tttagccgaa agcttccgtt tgcttgatga agaccaacaa gaaaaagtat
      241 atgctctttt ttcaaatgag gaattagctg attttttttc ttatctcgat gccgaagatg
      301 tttcggaaat caccgaaacc atcagcgaag aaaaactcgc ttccattatt caagaaatgg
      361 aacccgatga tgccgccgac ctcttggctg aatacagtga tgatcgagtg gaaatgattt
      421 atcaattcat cgacgaggat acgaaagaag aaataagtga attactagaa tatgaggaaa
      481 acacggccgg tgcccttatg aactccaatt atttggtgat tgagcaaggg aaagacatca
      541 aagatttaat gaaaaaactt gtaaaggaag caccggactt agaatcaatt aataccactt
      601 ttgttgtcga tgctaataac caattactgg gaacaattcc tttaaaaaaa gtaattatgg
      661 cgaaagcacc ctgtcccgtt gaaagtatca tgaatccgaa ttttaaagcc gtcaatgttg
      721 acacgcccat tgaagatgct gtcaatattg tgaaaaacta tgatattttt gatttaccgg
      781 ttttagaatc tggtcaatta aaaggcatta tcaccattga cgatgccatg gacaccgtcg
      841 ttgaggaagc cgaagaagac tacgctaaat tagccggttt aaccgaagcc gaagaaataa
      901 cggaatcaac ttttacatct gtaaaaaaac ggattccgtg gttggctttt cttttaataa
      961 tggatttatt tgtagcagtt gttatttccc aatatgatta cttatttgct cttgataaaa
     1021 tgaccgtttt tgtcttcttt caaccgatta ttttaggctt agccggtaat tttggaacgc
     1081 aatctttagc cattaccatt cgaaaaatta ctgaccatga gtttgattat cggcatcgta
     1141 ttttaaaaca cttaatcaag gaaaccgccc tcggtttagc catgggttta ctcttaggag
//
LOCUS       DAOSGN010000353         1189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_125795_length_1189_cov_3.414462, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000353 DAOSGN010000000
VERSION     DAOSGN010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125795_length_1189_cov_3.414462"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PK087_04780"
     CDS             complement(<1..619)
                     /locus_tag="PK087_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOH18619.1"
                     /translation="MARSYFFIKAHHHFILHNPHVYEADVIIINQNDAAFVFSHRNEF
                     PLLMEYLKTKEGELYVKVDDVDRKQFKANMKIIAGSLIKGWAIPKASCSLIKTMTIKV
                     RDFEQSHQLEFGSLNFIAIIDTPEGVLSYRKIANFERVKALFFDEEKYRTFLGWSQDS
                     NIDFVFQQVSFAASISKKPLIDRIKPSDSELIADLKRGKNLGCQFT"
     gene            complement(735..>1189)
                     /locus_tag="PK087_04785"
     CDS             complement(735..>1189)
                     /locus_tag="PK087_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH18620.1"
                     /translation="LGGFMSAKASVIEYVKHVSRPFIFSASITPASVQSARAALKILA
                     REPKRVKDLADISEYMRQGLINLGINIIKSSTPIIPIYTYEDEKTFLACKMLLNRGVY
                     VNPVISPATPVGQSLLRTSYTATHVKSQMDFAMKQIKEVLDILNPNEQ"
BASE COUNT          392 a          220 c          195 g          382 t
ORIGIN      
        1 tagtgaattg acagcctaag tttttaccac gctttaaatc ggcaatcaat tcactatcac
       61 ttggctttat tcgatcaatt aaaggttttt tactaattga agcagcaaaa gaaacttgtt
      121 gaaagacaaa atcaatatta ctatcttgac tccaaccaag aaaggtgcga tatttctcct
      181 catcaaaaaa caaagcctta actcgttcaa aattggctat ttttcgatat gaaagaacac
      241 cttcaggagt atcaataatg gcaataaaat taagtgaacc aaattctagt tgatggcttt
      301 gctcaaaatc acgaacttta atggtcattg tttttatcaa tgaacaagaa gctttcggaa
      361 tcgcccaacc tttaatcaaa gatccggcta ttattttcat attggcttta aactgttttc
      421 tgtcaacatc atcaactttt acatatagtt ccccttcctt cgtttttaga tattccatca
      481 ataaaggaaa ttcattgcga tgagagaaaa caaaagccgc atcgttttga ttaatgataa
      541 tgacatcagc ctcatataca tggggattat gaagaataaa atgatgatgt gctttgataa
      601 aaaaataact ccgagccatt tatatgccta ctttcgaaaa caatttttct taataaaaat
      661 catgttgcct ttatttttca tattatacca tagaataata ttcgtttcta atgaaaataa
      721 acctcgacgg aggtttattg ttcattcgga tttaaaatgt ctaaaacttc tttgatttgc
      781 ttcattgcaa agtccatttg tgatttaaca tgggttgccg tataggaagt tcttaacaag
      841 ctttgcccca ccggagtggc tggtgaaata accggattaa cataaacacc ccgattcaac
      901 aacattttac aagctaaaaa agtcttttca tcttcataag tataaatggg aataatgggc
      961 gttgaacttt taatgatatt aatccccaaa ttaattagtc cttggcgcat atattcgcta
     1021 atatccgcta aatccttaac tcgtttaggt tcacgggcta agatttttag agcggctcgt
     1081 gcactttgaa cactggctgg ggtaatagag gcactgaaga taaacggacg ggaaacgtgc
     1141 ttgacatatt cgatcactga cgctttggcg gacatgaacc ctcctaaag
//
LOCUS       DAOSGN010000354         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_125947_length_1188_cov_3.096205, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000354 DAOSGN010000000
VERSION     DAOSGN010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125947_length_1188_cov_3.096205"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..864)
                     /locus_tag="PK087_04790"
     CDS             complement(88..864)
                     /locus_tag="PK087_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH18621.1"
                     /translation="MKFCTIASGSKGNMTFVSTKATKIVIDAGISHLEAKKRLAGKDI
                     DLQGLSAVLITHEHGDHVKFLVTFLKKTQAHLYISALSYQKLDEEIKQQLTSFSVHFM
                     EANKRYQIGDLEFLTLKLSHDSVDILGFVFIEGSKRLAYVADTGFFPLHYLDLLKEVD
                     GLIIESNHDVEMLLESDRSTALKERILSPFGHMSNFICQQILSAVINDRHRVILLAHI
                     SEECNDFALIKRDIIDSLPSHEQLEIRVAGQWEASKVYEL"
     gene            complement(968..>1188)
                     /locus_tag="PK087_04795"
     CDS             complement(968..>1188)
                     /locus_tag="PK087_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18622.1"
                     /translation="TAAMKSAVSFFFTPVQNEITGSYYPNIQLFTQMKNLDISFKMNN
                     YSRAEFNQYLDLQIASSYDNLDYFTIGE"
BASE COUNT          375 a          230 c          208 g          375 t
ORIGIN      
        1 tccgcagcca atgccgacga tgataagcaa taataacaag aatgtcaata gtattttttt
       61 catatttttc tcctagcctt taactaatta taattcgtaa actttggaag cttcccattg
      121 tccggccacc cttatttcta attgttcgtg ggagggaagg gaatcaatga tatcgcgttt
      181 gataagagca aaatcattac attcctcact gatgtgagct aataaaataa cacggtgacg
      241 gtcattgata acggcactga gaatttgttg gcaaatgaaa ttggacatat gcccgaaagg
      301 tgacaaaatg cgttctttca aagccgttga acgatcactt tccaaaagca tttccacatc
      361 atgattggat tcaataatta aaccgtcaac ttctttcaac aaatctaaat aatgaagcgg
      421 gaaaaaaccg gtatccgcaa cataggccaa gcgctttgaa ccctcaatga acacaaagcc
      481 caaaatatca acactgtcgt gggataattt taaggttaaa aactcaagat cgccaatctg
      541 atagcgttta ttggcttcca taaaatgcac ggaaaacgaa gttaattgtt gttttatttc
      601 ttcatccagt ttttgataag aaagcgcact gatatataaa tgagcctgtg tttttttaag
      661 aaaggtcact aaaaatttta catgatcgcc atgctcatgc gtaatcaaca cggccgataa
      721 accttgtaaa tcaatgtctt tgcctgctaa tctctttttt gcttccaagt gggaaatgcc
      781 agcatcaatt acaattttcg tcgccttagt cgaaacaaag gtcatattac cttttgaccc
      841 gctggcaatc gtacaaaatt tcatcttcgt tcctcgtttt tactcttaac tattatacac
      901 aaattaatgg tttatttcaa gatttattta aaaagacctt gggtgttccc caaggtctta
      961 cttgctgtta ttcaccaatg gtgaaataat ctaaattatc gtaacttgaa gcaatttgta
     1021 agtcgagata ttgattaaat tcggctcttg aataattatt catcttaaag ctaatatcca
     1081 agtttttcat ctgggtgaat aattgaatgt tcggatagta ggaaccggtg atttcgtttt
     1141 gcaccggcgt aaagaaaaag ctgactgccg acttcatcgc tgctgtga
//
LOCUS       DAOSGN010000355         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_126020_length_1188_cov_2.043248, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000355 DAOSGN010000000
VERSION     DAOSGN010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126020_length_1188_cov_2.043248"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PK087_04800"
     CDS             complement(<1..553)
                     /locus_tag="PK087_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOH18623.1"
                     /translation="MAKIIRKETLNANVTLMDVEAPLIARKALAGQFIILRVDDHGER
                     IPLTIASADPHCGLVSVIFQIVGLTTAKLNLLNVGDDILDFVGPLGKPTNIKGIKKAC
                     VVAGGVGTAICYPLAQALFKQGTELDIIIGFRNKNLIILEEEFRRLSKNTFFITDDGS
                     YLRKGLVTDVLKEQLELGVRYDEV"
     gene            complement(537..>1188)
                     /locus_tag="PK087_04805"
     CDS             complement(537..>1188)
                     /locus_tag="PK087_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH18624.1"
                     /translation="MLHPDKTLNITTFISQALDANCYLVSDDAKMLVIDPCVSYDEVI
                     RHHQLPIAAVLLTHAHFDHFMELPSYLNKGIKLYLHRAALAKLADPLKSYSFYFNPLK
                     IDLLPSQYEVVFDYSRIFILSQSILVIETPGHTDCSLCFIIENNMFSGDTVFYHNIGR
                     TDLYSGNMVEMLKSLQKIRDFKQNYQIYPGHGQKTTLEQEIKSNPYFKRSFNGKNY"
BASE COUNT          387 a          226 c          221 g          354 t
ORIGIN      
        1 aaacctcatc atagcggact cctaactcca attgttcttt taatacatca gtgacaagcc
       61 ccttgcgaag ataggaacca tcatcagtga taaaaaatgt gtttttgctt aaacggcgga
      121 attcctcttc aagaatgatt aagtttttat tgcgaaagcc aatgataata tctagttcag
      181 tcccttgttt gaataaagct tgggcaaggg gataacaaat ggctgtaccc accccccctg
      241 cgacaacaca agctttttta atgcctttga tatttgttgg tttccccaac gggccaacaa
      301 aatctaaaat atcatcgcca acattcaaca aattgagctt agccgtcgta agcccaacaa
      361 tttggaaaat aacgcttact aaaccgcagt gagggtccgc actagcaatc gtgaggggaa
      421 ttctttctcc atgatcatca acacgcaaaa taataaattg accggctaaa gctttccttg
      481 caattaaggg agcttcaaca tccattaaag tgacattagc atttaaagtt tcttttctaa
      541 taatttttgc cattaaaact ccttttaaaa tatggattac tcttgatttc ttgttccaat
      601 gtcgttttct gtccatggcc aggataaatt tgatagtttt gtttaaaatc gcggattttt
      661 tggagtgact taagcatttc caccatattt ccggaataga ggtccgttcg tccaatattg
      721 tgataaaaaa ccgtatcacc actaaacata ttattttcaa taatgaagca aagcgaacaa
      781 tccgtatgac cgggtgtttc tatcactaaa atggattgag aaaggatgaa aatcctagaa
      841 taatcaaaaa ctacttcata ttgtgatggc agcaaatcta tttttagggg gttaaaataa
      901 aaggaatagc tctttagggg atcagccaat tttgctaggg cagcacgatg aagatataat
      961 tttattcctt tgttcaaata agaaggcaat tccataaaat gatcaaaatg cgcatgggta
     1021 agtaagacgg cagcaatcgg caactggtgg tggcggatta cttcatcata gctaacacag
     1081 gggtcaatca ctagcatttt tgcgtcatca gaaactaaat aacaattagc atcgagggct
     1141 tgtgatataa aagtcgtaat attaagggtt ttatcgggat gcagcata
//
LOCUS       DAOSGN010000356         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_126624_length_1184_cov_2.527901, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000356 DAOSGN010000000
VERSION     DAOSGN010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126624_length_1184_cov_2.527901"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..654
                     /locus_tag="PK087_04810"
     CDS             145..654
                     /locus_tag="PK087_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18625.1"
                     /translation="MNEKKLTYIVLSVAAFAILLVILLSIPWGGDKAAIDKEYDSLNG
                     RDHVFTTISFDSVMEKIAAGETFQVYIGSAALPTAEQFVYETNKLAKSLNVATIYYLR
                     SDKLTEDQITKIKIESSMAISFPTLIYWENDGATSTAFNISSLKDFSGSYQSNWSILL
                     SEYFNDCYK"
     gene            672..>1184
                     /locus_tag="PK087_04815"
     CDS             672..>1184
                     /locus_tag="PK087_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOH18626.1"
                     /translation="MKPKGFTNLVKWAKPYAWLVVFIILTTVLNPLLYSYVPQFIKYV
                     VDVIFGGTTEGSITLPSFLLDFFGSFNNQLTAVLVVGMSLLLFQIGRGSIMFFDGYNK
                     GRLAENIAYDMRKQMYSHIQQLPLSYHNNVDTGDLIQRCTSDIDTIKSFLSSQLPQIL
                     FIVGNFLAGAW"
BASE COUNT          332 a          202 c          242 g          408 t
ORIGIN      
        1 atgactgtta atcatggggt cataggttca agtcctattg ggggcgccat tttttatgct
       61 ttttcctttt ttcgagcatt gcaaacgatt agggcttatg ttataataat tgaggcttat
      121 caatcatgaa agcggaggat ccaaatgaat gaaaaaaaat taacttacat tgttttaagc
      181 gttgccgctt tcgcaatcct gctcgttatt ttgctttcca tcccctgggg tggagataaa
      241 gcggctattg ataaggagta tgacagcctt aatgggcgcg atcatgtgtt cacaacgatt
      301 tcttttgata gtgtgatgga aaaaattgct gctggtgaaa ccttccaagt ttacatcggt
      361 tctgccgctt taccaacagc ggagcaattt gtttatgaaa ccaacaagtt ggccaaaagc
      421 ctaaatgttg cgacgattta ttatttacga tctgataaac ttactgaaga tcaaattaca
      481 aaaatcaaaa ttgaatccag catggccatt agttttccta ctttgattta ttgggaaaat
      541 gacggtgcca cgagcacagc ttttaacatc agtagtttga aagatttttc cggaagttat
      601 caatcaaatt ggtcaatttt attaagcgaa tattttaatg attgttataa ataataggag
      661 aatttaatat aatgaaaccc aaaggattta ctaatttggt gaagtgggcc aaaccttatg
      721 cttggctggt tgttttcatc atcttaacga ctgttttaaa tcctttgttg tattcctatg
      781 tgccgcaatt cattaagtat gttgttgatg ttatttttgg aggtacaact gagggaagca
      841 tcacgcttcc ttctttttta ttagattttt tcgggtcctt taataatcag ttaacagcgg
      901 tcttagtggt cggaatgagt ttgctcttgt ttcaaattgg gcgcggttca attatgtttt
      961 ttgacggata caataaaggt cgcttagcgg aaaatattgc ctacgatatg cgtaaacaaa
     1021 tgtattccca catccagcaa ttaccacttt cttatcacaa caatgtcgat accggggatt
     1081 taattcagcg atgtacttcg gacatcgata ccatcaagag tttcttaagt tcgcagttgc
     1141 cgcagatttt atttattgtc ggtaatttct tggcgggagc ttgg
//
LOCUS       DAOSGN010000357         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_128391_length_1174_cov_1.814120, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000357 DAOSGN010000000
VERSION     DAOSGN010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128391_length_1174_cov_1.814120"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..880
                     /locus_tag="PK087_04820"
     CDS             137..880
                     /locus_tag="PK087_04820"
                     /inference="COORDINATES: protein motif:HMM:NF017001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH18627.1"
                     /translation="MKRTAFNRYNAGFMVNDNRRAKANKIMRVLNEAFGKREPTVSKD
                     TLLDIGTGNGEIASYLSAVYEVTSVDITDQRKIKNGFKFVKLIDTKLPFADQSFDIVI
                     SNHVIEHVKDAENHLSEIKRVLKDNGLVYLATPNRFWPWEVHNKIFFLHYLPKNTFNS
                     VLRNFGRHHEDIFPLTWWELRQRAKRHFSINVVSDRICKWPSRYHMQCHPCLGKLLAL
                     IPLNFYRILTFIHPTLIVVLKKSSDLSVR"
     gene            complement(857..>1174)
                     /locus_tag="PK087_04825"
     CDS             complement(857..>1174)
                     /locus_tag="PK087_04825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOH18628.1"
                     /translation="LLFPTVREGFGLAAAEAMACGLPVVATDCSSLPELIDEGNGGFL
                     CPLGDVDAFTYKINLLAENPQLRREMGEYNRAKVEKMFTLERMVNQYKELFEEVLSDR
                     KIT"
BASE COUNT          380 a          234 c          229 g          331 t
ORIGIN      
        1 aggtcaaagc gccttaaatt atgttcggca atactttaca tggaaagtga ttgcagaacg
       61 atatctgaat gtattttatg aagcgctgta ccaacaacct ccaaagaaat taatgtaaca
      121 aataagattt atgcaaatga agcgaacagc attcaatcga tacaacgccg gtttcatggt
      181 gaatgataat cgtcgtgcaa aagccaacaa aatcatgagg gtgctgaatg aagcctttgg
      241 aaaaagagaa ccaaccgttt ccaaggacac attgcttgac attggcaccg ggaatggtga
      301 aatcgccagt tatttaagtg ccgtttatga agttacaagt gttgatatta ctgatcagcg
      361 taaaataaaa aacggattta agtttgtaaa attaatcgat acaaagctgc catttgctga
      421 ccagtcattt gatatagtca ttagtaacca tgttatagaa catgttaagg atgcagaaaa
      481 tcacttatcg gagattaaga gagtcctaaa ggacaacggg ctcgtttatc tggccacccc
      541 aaatagattt tggccatggg aagttcataa caaaatcttt tttctccatt atttgccaaa
      601 gaacacattt aatagtgttc taagaaattt tggcagacac catgaagata tcttcccctt
      661 aacatggtgg gaactaagac aaagagcaaa aaggcacttt tcgataaatg ttgtgagcga
      721 ccgaatatgc aagtggccat cacgatatca tatgcaatgc catccttgtt tgggaaaatt
      781 gttggcttta atacctttaa atttttaccg tattttaact tttattcatc cgaccttgat
      841 tgtggtcttg aaaaaatcaa gtgatctttc tgtccgataa aacttcttca aacagttctt
      901 tgtactgatt taccatcctt tccagagtaa acatcttttc gacctttgcc cggttatatt
      961 ctcccatttc tcgacgtagt tgaggatttt cggcaagaag attaatttta taggtaaaag
     1021 catcgacatc acccagagga cataagaaac caccattgcc ttcgtcgatc aattccggca
     1081 aagacgaaca atcggttgca acaactggga gaccgcaggc catggcttca gccgccgcaa
     1141 gaccgaatcc ctcccgcacc gttgggaata gcag
//
LOCUS       DAOSGN010000358         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_128515_length_1173_cov_2.292487, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000358 DAOSGN010000000
VERSION     DAOSGN010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128515_length_1173_cov_2.292487"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PK087_04830"
     CDS             complement(<1..820)
                     /locus_tag="PK087_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18629.1"
                     /translation="MALNKIKSLLAAKIHGSHVKKHRQLVLRTHYLKGLSQENKLEDE
                     KRLEARYQMIETYYQEAIAPYQRQLSGITKLDDQKTKKILKFDEKAYWARRRYKTKQA
                     KTLDESKLEASLAQYDQRKRDERQAYLGKLEEKYPHVDLNEAELKQLQSQIDRQTMLR
                     DARKARALAQQTKKHAQFLKLKTTIAKKVEELKQQIKLLSEKIENYNQQKVKADILRR
                     EELLQAHKGLEANHHLPLHYKQKKLDVLASQIDLIDTTNLVLEDKTIHLAVSKLK"
     gene            complement(807..>1173)
                     /locus_tag="PK087_04835"
     CDS             complement(807..>1173)
                     /locus_tag="PK087_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18630.1"
                     /translation="LAFVISGVLGSIAGVLSMMYIRNASVSQIGLTFALNILAAVIIG
                     GTKSIWGILMGTFVIFGLDLAVFQRLELGNLSLIINGILIILVVMFYPGGLIQLVGDL
                     KKQFKIIKKKLREKIYGPE"
BASE COUNT          308 a          237 c          215 g          413 t
ORIGIN      
        1 tttttaattt cgataccgct aggtgaatcg ttttatcttc taaaactaaa ttagtcgtat
       61 caatcaaatc aatttggctg gctaaaacat ccagtttctt ttgcttataa tgcagcggaa
      121 gatggtgatt tgcttccagt cccttgtggg cttgtaataa ttcttcccgc cgcaaaatgt
      181 ccgctttaac tttttgctga ttgtaattct caattttctc acttaaaagc ttaatttgct
      241 gttttagttc ctcaactttt ttcgcaatcg tcgttttaag tttcaaaaat tgggcatgtt
      301 ttttcgtttg ttgagctaga gctcgggctt tacgagcatc acgaagcatt gtttggcgat
      361 caatttggct ttgaagctgc ttaagttccg cttcgtttaa atcaacatgg gggtatttct
      421 cctccagttt ccctaaatag gcctggcgtt catcccgttt tctttgatca tattgggcta
      481 agctagcttc caacttactt tcgtctaaag ttttggcttg cttggtttta tatcttcggc
      541 gcgcccaata ggctttttca tcaaatttaa gtattttttt agttttctga tcatcgagct
      601 tcgtaatacc actaagttgt ctttgataag gggcaatcgc ttcttgataa taagtttcaa
      661 tcatttggta acgagcttct aaacgttttt catcttccag tttgttttct tgggaaagac
      721 cctttaaata atgagttctt agcactaatt gacgatgttt ttttacatgt gatccatgaa
      781 tttttgctgc caaaaggctt ttgattttat tcagggccat agattttctc tcttaatttc
      841 tttttaatta ttttaaattg cttttttaaa tccccaacga gctggatcaa accgccggga
      901 taaaacatta ctactaaaat aattaaaata ccgttgataa tgagggataa attccccagt
      961 tccaaacgtt gaaaaacggc gaggtcaagc ccaaagataa cgaaggttcc catgagaatc
     1021 ccccatatcg acttggtgcc cccgataatc accgccgcta aaatgtttaa agcaaaggtt
     1081 aagccaattt gggaaacact cgcattgcgg atatacatca tggagaggac accggcaatc
     1141 gaccctaaga cgccggagat aacgaaggct agc
//
LOCUS       DAOSGN010000359         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_128797_length_1171_cov_3.399642, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000359 DAOSGN010000000
VERSION     DAOSGN010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128797_length_1171_cov_3.399642"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PK087_04840"
     CDS             complement(<1..667)
                     /locus_tag="PK087_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOH18631.1"
                     /translation="MNKINDALMCDFYEFTMSNGYFKFGLKDRICYFDVFFRSVPDGG
                     GFALACGLEQVIKYIQELHFSKDDIEYLRKKKIFSEEFLAYLLSFKFSGDIWAVPEGT
                     PIFPNEPIMTVRAKAIEAQLIETFVLTTLNHQSLIATKASRIVRAANGRAVFEFGARR
                     AHGPMAATLGARAAYIAGASGSSCTLTDYLFKTQAVGTMGHSWIQMFPDELTAFKRYC
                     ELYP"
     gene            complement(758..973)
                     /locus_tag="PK087_04845"
     CDS             complement(758..973)
                     /locus_tag="PK087_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOH18632.1"
                     /translation="MEYVKAFNGLSKLAKFLLTLFLDPIIAGIYRLIKGRYLIGAIWI
                     FTLGLFGIGWIIDLVTVVLDNKYSFLV"
BASE COUNT          414 a          244 c          198 g          315 t
ORIGIN      
        1 cgggatatag ttcacaataa cgcttaaaag ctgttaattc atcagggaac atctgaatcc
       61 aagaatggcc cattgtccct acagcctgcg tcttgaataa gtaatcagtt aaagtacatg
      121 aagacccgga agcaccagca atataggcgg cacgggctcc taaagtcgcc gccatcggtc
      181 catgagcccg gcgagcgcca aattcaaaaa cggcacgccc attagcagcc cgaacaatgc
      241 gggaagcctt ggtagcaatt aaggattgat gattgagagt tgttaaaaca aaggtttcta
      301 ttaactgagc ttcaatggct ttagctctaa ccgtcataat tggttcattc gggaaaatgg
      361 gagttccttc gggaacagcc caaatatctc cactgaattt aaatgataat aaatatgcta
      421 aaaactcttc cgaaaaaatc ttcttctttc gtaaatattc gatatcatcc tttgaaaaat
      481 gcaattcctg aatgtatttg atgacttgtt caagcccgca agcaagagca aaaccaccgc
      541 catccggaac agaacgaaaa aagacatcaa aatagcaaat gcgatccttt aagccaaatt
      601 taaaataacc attgctcata gtaaattcat aaaagtcaca cattaaggca tcattaattt
      661 tgttcatggt aagctccttt ttttaatgat tataccatat ttgggaaagg ttttgaatgc
      721 ttaaattaaa aaaaccggcc aagccggctt tagaaaatta tacaagaaaa gagtacttgt
      781 tatcaagaac taccgtcaca agatcaataa tccaaccgat tccaaaaaga ccaagagtaa
      841 aaatccaaat agcaccaatc aaatatctgc cttttataag tcggtaaatt ccggcaataa
      901 tcgggtcaag gaataaggtt aataaaaatt tagctaactt gcttaaacca ttaaatgctt
      961 ttacatattc catttgtgct cctccttttc taaatattca tttattcatc taagctttca
     1021 taagcttcaa tggtcttagt gggaattggt tttgaatcac catgttttac aaacaacatc
     1081 gttataaaag ccaagacaca gaaaaccgca ctataagcaa acaaggactt catcccaatt
     1141 aaatccatca tcgctccgga taaaatgggc g
//
LOCUS       DAOSGN010000360         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_128901_length_1171_cov_1.720430, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000360 DAOSGN010000000
VERSION     DAOSGN010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128901_length_1171_cov_1.720430"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1171)
                     /gene="dnaK"
                     /locus_tag="PK087_04850"
                     /pseudo
     CDS             complement(1..1171)
                     /gene="dnaK"
                     /locus_tag="PK087_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421611.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
BASE COUNT          338 a          243 c          190 g          400 t
ORIGIN      
        1 cttgattctt taagcatttt ttcggtcgtg aaaataaccg cttcggtttg atttttaatt
       61 tcaatttctt cttttttctt tttatcatct tcagcatgca tttccgcttc ttttttcata
      121 cgttcaattt cttctttgct caaaccagaa gaagcagtaa tagtagtatt ttgttgttta
      181 ttagttgcct tatcagtagc ggaaacattt aagatgccgt tttgatctaa atcaaatttt
      241 acttcaattt gcggaattcc gcgcggtgca ttaggaatgc catccaaaat aaaacgacct
      301 aaagttttat tatcacttgc taaaggacgt tcaccttgaa caatatgaat ttccacactg
      361 gtttgaccat cagcggcagt ggaaaatact tgtgatttag aagtgggaat agtagtgttt
      421 ctttcaatta acggtgtagc gactccgccc aaagtttcaa ttcctaaagt aagcggggta
      481 acatcaagta ataaaacatc tttcacatca ccttgtaaaa cacccgcctg aacagcggcg
      541 cccaaagcta ctacttcgtc cggattaaca tttaagttag gttctttgcc aaaaaattct
      601 ttaacttttt gttgaaccaa gggcatacga gtcatacctc caactaatag cacttcatta
      661 atctcgttaa tttgaaaacc ggaatcagta attgctttac gacaaggttc aatggttttt
      721 tccactaaat caactaccaa agattctaat ttggaacgat taatttcatg accaaatgtt
      781 ttggcccatt aacatcagtg gtgatgaagg gttgattgat ttctgtttct tgggcagttg
      841 ataattcaat cttggctttt tcggcggctt ctttaattct ttgcaaagcc aaagaatcct
      901 tagacaaatc aattccctga tcgcgtttaa attcctcaat aatccaatca atgattcttt
      961 ggtcgaaatc ttcaccacct aagtgagtat caccattagt cgctttcact tcaactgtat
     1021 cagcggaaat atcaagcacg gagacatcaa atgttccacc gcctaaatca tagacaacaa
     1081 ttttttgccc attcttttta tcaaaaccat aggctaaagc agccgccgtt ggttcgttaa
     1141 tgattctttt tacttccaaa ccggcaattt g
//
LOCUS       DAOSGN010000361         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_129842_length_1165_cov_4.686486, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000361 DAOSGN010000000
VERSION     DAOSGN010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129842_length_1165_cov_4.686486"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /gene="rpsQ"
                     /locus_tag="PK087_04855"
     CDS             <1..226
                     /gene="rpsQ"
                     /locus_tag="PK087_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOH18633.1"
                     /translation="VVLDKADKTIKVLYTTFHRHPLYGKRVEVSKKLTAHDEKNEAKI
                     GDTVRIVETRPLSKTKHFRLLEVIERAEIV"
     gene            238..609
                     /gene="rplN"
                     /locus_tag="PK087_04860"
     CDS             238..609
                     /gene="rplN"
                     /locus_tag="PK087_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOH18634.1"
                     /translation="MIQQETRLKVADNSGAKEILTVKVLGGTSRRYANIGDIIVATVK
                     SATPGSAVKKGELVKAVIVRTKSGLRRKDGTYIKFDDNAAVLIRDDKTPKGTRIFGPV
                     ARELRDVTEFMKIISLAPEVL"
     gene            620..931
                     /gene="rplX"
                     /locus_tag="PK087_04865"
     CDS             620..931
                     /gene="rplX"
                     /locus_tag="PK087_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOH18635.1"
                     /translation="MKIKKGDTVKIIAGNSKGKTGKVLQVLPKENKVVVEGIAVVSRH
                     TKPSNAHPDGGIIKKEAAIDLSNVQYFDTKGNVSTKVGHKFVDGKKVRVAKKTGNVLD
                     K"
     gene            942..>1165
                     /gene="rplE"
                     /locus_tag="PK087_04870"
     CDS             942..>1165
                     /gene="rplE"
                     /locus_tag="PK087_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOH18636.1"
                     /translation="MNRVQKDYIENVKPKLMKKFGYTSIMQVPKLEKIVVNMGVGDAI
                     SNAKLLDAAVDDLTTISGQKPVISKAKKSIA"
BASE COUNT          404 a          208 c          263 g          290 t
ORIGIN      
        1 agtagtattg gataaagcag ataaaacgat taaagtgctt tatactacct ttcatcgcca
       61 tcctttatat ggaaaacgcg ttgaagtttc taaaaaattg acggctcatg atgaaaagaa
      121 tgaagctaaa atcggcgaca ccgtgcgcat tgtggaaaca agaccgcttt caaaaacaaa
      181 acatttccgc ttgcttgaag ttatcgagcg cgctgagatt gtttagggag gataatcatg
      241 attcaacaag aaacaagact gaaagtggcc gataattcgg gtgctaaaga aattctaacg
      301 gtcaaggttt taggcggaac atcgcgccgt tatgccaaca ttggtgatat tattgtcgcc
      361 acggtaaagt cggcaactcc cggcagtgcg gttaaaaaag gtgaactagt aaaagcagta
      421 attgtgagaa ccaaatcagg attaagaaga aaagatggaa catacatcaa gtttgatgac
      481 aatgccgccg ttttaattcg tgatgacaaa accccgaaag ggacacgaat ttttgggccc
      541 gttgctcgcg aactacggga tgtaaccgag ttcatgaaaa ttatttcttt agctcctgag
      601 gttctataag gaggacctca tgaaaataaa aaaaggtgat accgttaaaa taattgccgg
      661 aaacagcaaa ggcaaaaccg gcaaggtttt acaagtgctt ccgaaagaaa ataaagttgt
      721 agttgagggc attgcggtcg tctcacgcca caccaaacct tccaacgctc atcccgatgg
      781 tggaattatt aaaaaagaag cggccattga tctttccaat gtccaatact ttgataccaa
      841 agggaatgtg tcaaccaagg ttggacataa gtttgttgat ggcaaaaaag taagagtcgc
      901 caaaaaaacc ggcaatgtct tagataagta ggaggaagac gatgaatcga gttcaaaaag
      961 attatatcga aaatgtcaaa cccaaattaa tgaaaaagtt tggctatact tccattatgc
     1021 aagtgccgaa actggaaaaa attgttgtta atatgggtgt tggcgatgct attagcaatg
     1081 ctaaattatt ggatgcggct gtcgatgact taacgacgat ttccggtcaa aaaccggtta
     1141 tttcgaaagc taagaaatcc attgc
//
LOCUS       DAOSGN010000362         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_130535_length_1161_cov_3.494575, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000362 DAOSGN010000000
VERSION     DAOSGN010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130535_length_1161_cov_3.494575"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PK087_04875"
     CDS             <1..400
                     /locus_tag="PK087_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOH18637.1"
                     /translation="IIAMRLQLKIGAPIYYVERLRHAQNIPLAYERVYLPVNMYDNIT
                     PDKFIDSFYDYVQNELNWKIRNCVRAIEARGLTKNVADLLHQKEGEPSLYMSSVTYLD
                     NGRAFVYTRCYFHAEHFRFKHNFSRAENNH"
     gene            400..>1161
                     /locus_tag="PK087_04880"
     CDS             400..>1161
                     /locus_tag="PK087_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOH18638.1"
                     /translation="MYEMSSLTWFNPLNEAAMSLYGFNWLKDDQVYRRLPLASKESIK
                     QACPGINDLAEKTSGGQLHFYTNSDCLYIRAKTKGQAQMAGMTAVACGGFDCYAGKNY
                     TDLRFYNTAYFDSHRQGYEAMVFENEPGFKLLVINFPLYGGIEHLEIGLKTGSKIKKP
                     EPLQPGRIVIYGTSITQGGCASRPGLSFTNFLSRRLNQEFLNFGFSENAFGEIALASI
                     FKTIDNVSLFIIDYEANAGTNGKMAQTLEAFIKELR"
BASE COUNT          336 a          213 c          258 g          354 t
ORIGIN      
        1 aatcattgcc atgcgtctcc agttaaaaat cggggccccg atttattatg tggagcgttt
       61 gcgtcatgca caaaacattc cgctggcata tgaacgagta tatttaccgg ttaatatgta
      121 tgacaatatt actccggata aattcattga ttccttctat gattatgtac aaaatgaatt
      181 gaattggaaa ataagaaact gtgttcgggc cattgaagcc cgcggtctaa ccaagaatgt
      241 ggctgattta ttgcatcaaa aggaaggaga accgtccttg tatatgagta gtgtgactta
      301 tttggataac ggtcgggcgt ttgtttacac acgctgttat ttccatgcag aacatttccg
      361 ttttaagcac aacttttcac gggcggaaaa taatcactaa tgtatgaaat gagttcatta
      421 acatggttta atccccttaa cgaagcagcg atgagccttt atggatttaa ttggttgaaa
      481 gatgaccaag tttatcgtcg cttacctttg gcttcaaagg aaagcattaa acaagcttgt
      541 ccgggcatta atgatttagc ggaaaaaact tccggcgggc aactgcactt ttataccaat
      601 tcggattgtc tttatatccg tgccaagacg aaagggcaag cccaaatggc cgggatgacg
      661 gcggtggctt gtggcggttt tgattgctat gccgggaaaa attatacgga tttgcgtttt
      721 tataacaccg cttattttga ttcgcatcgg caaggatatg aagcgatggt ctttgaaaat
      781 gaaccgggat ttaagctttt ggtcatcaat tttcctttgt atggggggat tgaacattta
      841 gaaatcggtt taaaaacggg aagtaaaatt aaaaaacccg agcctttaca accgggtcgg
      901 attgttattt atggaacttc cattactcaa ggtggctgtg ccagccgtcc gggtctttcc
      961 ttcaccaatt ttttaagtcg ccgcttaaac caagaatttt taaatttcgg gttttccgaa
     1021 aatgcttttg gggaaatcgc cttggcaagc atttttaaaa cgattgacaa tgtttcactt
     1081 tttatcattg attatgaagc aaatgccgga acaaacggca aaatggccca aacgctggaa
     1141 gcttttatta aagaattacg g
//
LOCUS       DAOSGN010000363         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_130865_length_1159_cov_3.836957, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000363 DAOSGN010000000
VERSION     DAOSGN010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130865_length_1159_cov_3.836957"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..>1159)
                     /locus_tag="PK087_04885"
     CDS             complement(275..>1159)
                     /locus_tag="PK087_04885"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOH18639.1"
                     /translation="IPLAECTLTRFADGEVNINIAETVRGHHVFVFQSTSEPVNDHYM
                     ELLIMMDALRRASARTINVIMPYYGYSRQDRKALARQPISAKLVADLLTVAGATRVVS
                     MDLHAGQIQGFFNIPIDNFEAAPVMVRYIKEKKLEDIVIVSPDHGGTTRARKFALNFN
                     APIAIIDKRRPRPNVAEVMNIIGEVEDKNCIIVDDIVDTAGTITIAADALKAAGAKDV
                     YVIATHPILSGDACNRIQNSQITELITTNSIRLKECKKIPKIRQLSVARIFAQGIMNI
                     IEDKPISDLFNYNPLNKF"
BASE COUNT          346 a          252 c          211 g          350 t
ORIGIN      
        1 cattatttgt ttatctcctg ctccacctgt tctatttagt tttcaaagac cttatgctta
       61 gctcttcttt agagcgtggt attattatac aataatcacc actttattgt caacaacttt
      121 aactgctttt cccgtaaaac ggcttagttg ttgtccttaa ccttagatat tatactacaa
      181 accacttttt atgtcaaaca aaatcgtgct ttttcgctcg tgacaaagac attaaaaaaa
      241 gcggttaaaa accgcctttg aactgttaat tcaactaaaa tttatttaaa ggattgtaat
      301 taaataaatc actaattggt ttatcttcaa taatgttcat aatgccttga gcaaaaatac
      361 gagccaccga taactggcgg attttcggaa tctttttaca ttccttcaaa cgaatagagt
      421 ttgtagtaat cagttccgta atctgtgaat tttgaatacg gttgcaagca tcaccagaaa
      481 gaatcggatg cgtcgcaatg acataaacat ctttggcgcc agccgctttc aaagcatctg
      541 ctgcgatggt aatcgttccc gccgtatcga cgatatcatc aacaataata cagtttttat
      601 cttcaacttc cccaatgata ttcatcactt ccgcaacatt gggtcgcggg cgacgtttat
      661 caataatggc aatcggagca ttgaaattta aagcaaattt gcgggctcta gtcgttccgc
      721 catgatcggg agaaacaatg acgatgtcct ccagcttttt ttctttaata tagcgaacca
      781 tgaccggagc ggcttcaaaa ttatcaatcg ggatattaaa gaagccttga atttggccgg
      841 catgaagatc catgctgaca accctcgttg ctccggcaac cgtcaacaaa tcggcaacta
      901 acttggccga gatgggttga cgagcaaggg ctttacggtc ttgccgtgaa taaccataat
      961 aaggcatgat gacattaatg gttcgggctg atgctcgtct taaagcatcc atcataatca
     1021 acagctccat gtaatgatca ttgaccggtt ccgatgttga ttgaaaaaca aacacatgat
     1081 gtccacgcac tgtttccgca atattgatgt taacttcacc atcagcaaag cgtgttaaag
     1141 tacactccgc caagggaat
//
LOCUS       DAOSGN010000364         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_131400_length_1156_cov_3.372389, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000364 DAOSGN010000000
VERSION     DAOSGN010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131400_length_1156_cov_3.372389"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..>1156)
                     /locus_tag="PK087_04890"
     CDS             complement(83..>1156)
                     /locus_tag="PK087_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOH18640.1"
                     /translation="TFAIRIIDNSISIDFQQAVIDVIYQTKSSILGVSNYVFDSTLNQ
                     YKKSSIGSGFVYEVWFELTDKSIVHSIDDLVTFDDVEKYCYYLVTNKHVVEGSDALKI
                     YLSEEDREIPATLMQYDDKVDLAVVYFEHNRYLKPLVLGDSDLMQSGEFAIAIGNPSG
                     YEYSSSATFGIISHPKRYISDDTDDDGINDWDAEYIQHDVAINPGNSGGPLLNLRGEV
                     IGVNTMKFAAADIDNMGFSIPSNVVSGLVDLLEQGLKPTRAKIGVTVMEVVALRESPQ
                     PDIVLPDEATFGLFVAEVSPNSVGAKGGILPNDIILTFNGVELFRSLALRAELGKIIV
                     GSGQEIEVTVLRNGEILTLTLIF"
BASE COUNT          402 a          236 c          191 g          327 t
ORIGIN      
        1 caaaacttag aatgtcataa aaaacaaacc ccatgcgaaa atggggtttg tttcatgatg
       61 actatcaaaa agtcagttat tattagaata tcaaagtaag ggtcaaaatt tccccgtttc
      121 ttaaaacagt aacttcaatt tcttgaccgg aaccaacgat tattttacca agttccgctc
      181 ttagagcaag ggatctaaat aattcaacac cgttaaatgt caaaatgatg tcattcggta
      241 aaatgccgcc tttagcaccg acactattag gagaaacctc agccacaaaa agaccaaagg
      301 tcgcttcatc gggcaaaact atatccggtt gtggcgattc acgaagagca acaacttcca
      361 ttacagtgac accaatttta gcacgcgttg gttttaagcc ttgttctagt aaatccacta
      421 acccagaaac gacattgctg gggatggaaa atcccatatt atcaatatcg gcagcagcga
      481 atttcatggt attaacaccg atgacttcgc ctcttaaatt taataatggt ccaccactgt
      541 tacctggatt aatggcaaca tcgtgttgaa tgtattcagc atcccaatca ttaatgccat
      601 catcatccgt atcatcggat atataacgtt tgggatggga gataatgccg aaagtggcgg
      661 aagaagaata ctcatacccc gaaggattac caatagcaat ggcaaattct ccactttgca
      721 ttaaatcaga atcccctaaa acaagcggtt tgaggtaacg gttgtgttca aaataaacaa
      781 ctgccaaatc aactttatca tcatattgca ttaaggtagc cggaatttct ctgtcttctt
      841 ccgataaata aatttttaat gcatcggatc cttccacaac atgtttattg gtgaccaaat
      901 aatagcaata tttttctaca tcgtcaaacg tgaccaaatc atcaatgcta tgaacaatac
      961 ttttatcagt taattcaaac caaacttcat aaacaaaacc actaccaatg gagctctttt
     1021 tatattggtt tagagttgaa tcaaatacat aattagagac cccaagaata gaacttttgg
     1081 tttgataaat gacatcaata acggcttgtt gaaaatcaat gctaatggaa ttatcaataa
     1141 tccgaatagc gaaagt
//
LOCUS       DAOSGN010000365         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_131402_length_1156_cov_3.355132, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000365 DAOSGN010000000
VERSION     DAOSGN010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131402_length_1156_cov_3.355132"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PK087_04895"
     CDS             <1..342
                     /locus_tag="PK087_04895"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HOH18641.1"
                     /translation="LVYEIGMTPITRPLVIKSTIRNTHYLSGIILIAQSHISLHYDYE
                     NKVIYFDIFSCASFDFSMVTNVLSDLGKVTSYEVVCRGTKHYSKVKHEIDNTEAIASE
                     KWQKNIYNDCL"
     gene            complement(456..>1156)
                     /locus_tag="PK087_04900"
     CDS             complement(456..>1156)
                     /locus_tag="PK087_04900"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH18642.1"
                     /translation="KLHSLLIVSGAFGLFHRPTLINAGGYLTSSGIYKKDTVGEDMEL
                     VVRLSRKALEMKFKYHVDYVYNAVCYTELPNDWKSLLKQRNRWHRGLIDILSYHRKLI
                     FNPRYKHVGTIGFPYFLIYETIGPLFEVQGFLMLLVSLVFGLLNLEIILSLFIVTIMM
                     GIIISLLALYVTERESKYLGFRDTLLLILFSFLENLGYRQFISLDRVRGFFAALNETG
                     AWGTATRAGFKKTS"
BASE COUNT          399 a          208 c          215 g          334 t
ORIGIN      
        1 ctggtttatg aaattggcat gacgccgatt acacgtccat tggttattaa aagcaccatc
       61 cgcaacactc actatttatc ggggattatc cttattgcac aaagtcatat ttcactccat
      121 tatgattatg aaaacaaagt catttatttt gacatttttt cctgtgcttc ctttgacttc
      181 tcaatggtta ctaatgtatt aagtgattta gggaaggtaa cttcctatga agttgtgtgc
      241 cgaggcacga aacattattc aaaggtcaaa catgaaattg acaatactga agccattgct
      301 tcggaaaaat ggcaaaaaaa catttataat gattgcttat aaaaaacacc accaaaaggt
      361 ggtgaaccgt tctcttgaag aacggttttt tgtttagggt gtgggtttta aaaaacacta
      421 acaatcgtta gggaagatga tttaataagg aatttttaag aggttttttt aaagcctgcc
      481 cgcgtcgcag ttccccatgc gccggtttca tttagggcag caaaaaagcc ccgtacacgg
      541 tccaaactga taaattggcg atagcctaaa ttttctaaaa agctgaataa aatcagcaac
      601 aaagtatcgc gaaatcccaa atatttcgat tctcgctccg ttacatacag ggctaatagg
      661 gaaatgataa ttcccatcat gatagtaaca atgaacaaag aaagaatgat ttctaggttt
      721 aacaaaccaa aaactaaaga aaccaacagc attagaaatc cttggacttc aaacaagggg
      781 ccaattgttt cataaattaa gaaatacgga aaaccgatgg ttccaacatg tttatagcga
      841 ggattaaaga ttaatttgcg atgataggat aaaatatcaa ttaatccacg gtgccaacga
      901 ttgcgttgtt ttagtaaact tttccaatca ttagggagtt ccgtataaca aacggcatta
      961 taaacataat cgacatggta tttaaacttc atttccaaag cttttcggga gaggcgaaca
     1021 acgagttcca tatcttcacc aacagtatct tttttataaa tgcctgaact ggttaaataa
     1081 ccgccggcat tgattaaagt tgggcggtgg aacaatccaa aagctccgga aacaattagt
     1141 aaagagtgta atttgg
//
LOCUS       DAOSGN010000366         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_131416_length_1156_cov_2.834696, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000366 DAOSGN010000000
VERSION     DAOSGN010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131416_length_1156_cov_2.834696"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PK087_04905"
     CDS             complement(<1..456)
                     /locus_tag="PK087_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH18643.1"
                     /translation="MKIRIVVDSTFRLPPEYVAKHQLEVVPLNVIIDNQTLTDEVDID
                     LGGVLKAVYDGRKVSTSQPSPAAFKRAFHKLAQAGATDILCVTISSTLSGTFNAAQLA
                     AQEITNVNIHVFDSLSTSIGGEMLVKILVEQVELGKTIPEAIEYCLPFQA"
     gene            complement(761..>1156)
                     /locus_tag="PK087_04910"
     CDS             complement(761..>1156)
                     /locus_tag="PK087_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18644.1"
                     /translation="NIGQAGTDFSGALSTNSYQEYGLNITISHNVFETCTNYLWVRNN
                     ATADNHANYPWVGKVEYNAFLGEPLVYYHHNRNATDTEITNPALIEFAYNFFGDASGK
                     VISLDMNKFLAVKSVTNNFNSLEELNAAS"
BASE COUNT          385 a          233 c          203 g          335 t
ORIGIN      
        1 agcctgaaac ggtaaacaat attcaatggc ttcgggaatt gtttttccca actcgacttg
       61 ttcaaccaat attttcacaa gcatttcgcc accaatcgaa gtggaaagcg aatcaaacac
      121 atgaatattg acattagtaa tctcttgggc cgctaattgt gctgcgttaa aagtcccgga
      181 tagggtggaa gaaatcgtta cacataaaat atctgtcgcc ccggcttgcg ccagtttgtg
      241 aaaggcacgc ttaaaagcgg ccggggaagg ttggctggtt gagactttcc ggccatcgta
      301 aacagctttt aaaaccccac ccaaatcaat atcgacttca tccgtcagcg tttgattatc
      361 aataatgaca ttgagcggaa caacttccaa ttggtgtttg gccacatatt ccggtggtaa
      421 acgaaaagta ctatcaacaa caatgcgaat tttcatggtt ttctcctatt attccaagtt
      481 tcatcatata tcttattata gacgatattt attttttttc tttaaaaatc caccttaaaa
      541 aatgacaatt tcccctaata tgttaagaaa aaacgaagtt catcaagatg aaggcttaaa
      601 tcatcacttt tcagcaaaaa aggtttccgc ttttaacgaa aaccttttaa actgaattat
      661 cacaaaatct ttagacgact ttataaagct ataaagaagc aaaagtttcc aaaacaacaa
      721 taactaccat ccattaaaga agttaaaact ttaataatcg ctacgaagcc gcattcaatt
      781 cttcaagcga attgaaatta ttagtaacac ttttaactgc caaaaattta ttcatatcaa
      841 ggctaatcac tttaccactg gcatcgccga agaaattata agcaaactcg attaaggccg
      901 gattggtaat ttcggtatcc gtagcattgc ggttatgatg ataataaact aagggttcac
      961 ctaggaaagc attgtattca accttcccca cccagggata attggcatgg ttgtcagcag
     1021 ttgcattatt gcgcacccat aaataattag tacaagtttc aaagacatta tgagaaatgg
     1081 tgatattaag accatattct tggtaggagt ttgtggaaag ggcgccggag aaatccgtcc
     1141 ccgcttgacc aatatt
//
LOCUS       DAOSGN010000367         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_131430_length_1156_cov_2.613079, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000367 DAOSGN010000000
VERSION     DAOSGN010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131430_length_1156_cov_2.613079"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..>1156
                     /locus_tag="PK087_04915"
     CDS             191..>1156
                     /locus_tag="PK087_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18645.1"
                     /translation="METSDKKVKSAHSRTAGTWFKDFLSTIGGFFVRLGKGIVNFFID
                     TGIAIKNTFVNLWKMIKNFFVSVYTHFVKGGWRTKLSHFIMGSGQMSMGRPIKGIIYL
                     LIQVAYFIFMFVPGGGLAWLIKFRTLGEVAVEYMAECTWPPGAPIEDCDPDFITYEKP
                     IFKDNSLLIMLFSVATILITVFFIVIYFVQVRGTFKAEKAYNEAKAKYELAKSEGSEE
                     AFKNPLPKFTDELKSLLNERFHFTTLSLPAITLTMFTILPLVFMILLAFTNYNRDTAP
                     PNKLFTWVGLDTFKNLFSTKGASATNATFGSAMVRILKWTFTWAFF"
BASE COUNT          365 a          212 c          212 g          367 t
ORIGIN      
        1 acttcttatg gaagggtcca gcaagtgaag aatcaccaca aacacttcaa gcaacacttg
       61 atgcctttgt ggatgcctta acaaaataat ataaaattat taaacacata taatcgatta
      121 atttttaatt aattttggga gccagttcat cgctggctct cctatctttc tagaaatgag
      181 gccataaaat atggaaacaa gtgataaaaa agtcaaatcg gcccactcac gtacggctgg
      241 cacttggttt aaagatttct tgtccacaat tggtggtttc tttgtgcgtt taggcaaagg
      301 aatcgtcaat ttttttattg atacaggaat tgccattaaa aacactttcg ttaatttatg
      361 gaaaatgatt aaaaactttt ttgtatcagt ttacacacac tttgtcaaag gaggatggcg
      421 cactaaactc tcgcacttca taatgggaag tggacaaatg tcaatgggcc gtccaataaa
      481 aggcattatt tatcttttaa tccaagtagc gtatttcatt ttcatgttcg tcccaggagg
      541 aggactagca tggttaatta aatttagaac actcggagaa gttgcggttg aatatatggc
      601 ggaatgtact tggccaccag gtgctcctat tgaagattgt gacccagatt ttattacata
      661 tgagaaacct atctttaaag ataactcgct cttaattatg ttattctcgg tcgcaacaat
      721 tttaattacc gtctttttca tcgtcattta ctttgttcaa gttcgcggta cttttaaagc
      781 agaaaaagct tataacgaag caaaagcaaa gtatgaactt gcgaaaagtg aaggtagtga
      841 agaagcattt aagaacccac ttccaaaatt tactgatgaa cttaagtcat tattaaatga
      901 aaggttccac ttcacgacac tctcactccc ggcaataaca ttaacgatgt ttacgatatt
      961 accgcttgtc tttatgattt tgctcgcgtt tactaactat aaccgcgaca ctgccccgcc
     1021 gaataaatta tttacttggg ttgggttaga cacatttaaa aatttattta gtacaaaagg
     1081 cgcttccgct actaatgcga catttggtag tgcgatggta agaatcttga aatggacatt
     1141 tacctgggca ttcttt
//
LOCUS       DAOSGN010000368         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_131951_length_1153_cov_2.677596, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000368 DAOSGN010000000
VERSION     DAOSGN010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131951_length_1153_cov_2.677596"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..209
                     /locus_tag="PK087_04920"
                     /pseudo
     CDS             48..209
                     /locus_tag="PK087_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
     gene            294..845
                     /locus_tag="PK087_04925"
     CDS             294..845
                     /locus_tag="PK087_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOH18646.1"
                     /translation="MKTSTIPEGVVEIAENAFEYARFKTITIPNTVTSIGDGAFAYSS
                     ALEDITIPDSVTNLGADAFRSCSALKTVTLGSGLKSIKTWTFNWCENLTSVTMTDSIT
                     YIGDSAFTGCVLLASITLPDSLVLIDDSAFAGCAVLATIKLPSSVESIGSGAFNGCDA
                     LMSLLIPPASNIFLEKYPIVKII"
BASE COUNT          349 a          203 c          232 g          369 t
ORIGIN      
        1 aatgttcatc ctgattgtaa gattattgcc ggtggagctt ttatcataat aagttggaaa
       61 gtgttacctt ccctgaaggg ctgatgcata ttggtgatga agcattaggt tggtgtcata
      121 atttaaaaac gaaagtaatc atccccaaga gtgtgaaaac aatcggttcc aagttattaa
      181 gtgcttgttc caatttaaca gaacttgaag ttgccgccgg gaatactgtc tatgattcga
      241 gagataattg caatgccatt attgaaacag ctaccaataa gctaattgca ggaatcaaga
      301 catcaacgat tccggaaggg gttgtggaga ttgcggaaaa tgcttttgaa tatgctcgct
      361 ttaaaacaat tacgattcct aacaccgtta caagtattgg cgatggcgcc tttgcttatt
      421 cttcggcttt ggaagatatt accatccccg acagtgtcac aaacttagga gctgatgctt
      481 ttagaagttg ttcggctctt aaaacagtta cccttggcag tggtcttaaa tcaattaaaa
      541 catggacctt caattggtgt gaaaacttaa catcagtaac gatgacggat tcaattacct
      601 atattgggga cagcgccttt accggatgtg ttttattagc atcaattaca cttcctgata
      661 gccttgtttt gattgatgat tcggcttttg ccggttgtgc cgttttagca acaattaaac
      721 tccccagcag tgtggagtcg attggcagtg gtgctttcaa tggctgtgat gctctaatgt
      781 ccttgcttat tcctccagcg tcaaacatat ttttggaaaa atatcctata gtaaaaataa
      841 tttagaaaca ttttattgtg aagcaacatc tacaccacag ggcttctttg ctgattggca
      901 taacgaaaaa actgtttatt ggtatagcga aacagaaaac aaagacggtg ctcattggcg
      961 ttatgttgat ggtgaaccaa gcatttggtc ttaaaactgt tatcgtaaaa taagtgattt
     1021 gtccttcagg tagtaactac aaacaaatta tttaattaag tttgcttatt ttctcaattc
     1081 ccattataat aagcttacta caaatgaatg agggactcac aaatgtcatt taaattaaag
     1141 tttatccatg atg
//
LOCUS       DAOSGN010000369         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_132666_length_1149_cov_2.382998, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000369 DAOSGN010000000
VERSION     DAOSGN010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132666_length_1149_cov_2.382998"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PK087_04930"
     CDS             <1..1010
                     /locus_tag="PK087_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOH18647.1"
                     /translation="FTCLLPISQVAVPEEERGNLIGQTIEVAPIRIDYGRIRLIVSQN
                     VANAIKKRNIKKEFLEQIQVGQIFEGTVKNVETYGAFVELGNGVEGLLHISEYDHVRI
                     FKLDRVVKPGDKVQVQVIKIDNEHVGLSRKALIPNFWQEYTESKEVGQVINGKVIEIN
                     NAGLVMELSPEVTGFLPKSEFAYERDVYISDTVNVGDQIDAKIIEIDPNKRRIILSKK
                     QLTANPWDDLKIKAGDNVEVLVTKELKDGFKVELNGATGYLPKSNIFNYPVEDIVAGS
                     QLKVKVRVFDPEKTRLVVGLKVETERIERESYNKFMKAQDKLTNNFGDLLANKFKDSH
                     K"
     gene            1018..>1149
                     /locus_tag="PK087_04935"
     CDS             1018..>1149
                     /locus_tag="PK087_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOH18648.1"
                     /translation="MRGVVAIVGRPNVGKSSLFNRIVGERLSITDDASGITRDRIYSK
                     "
BASE COUNT          402 a          172 c          236 g          339 t
ORIGIN      
        1 aatttacttg cttgctcccc attagccaag tagctgttcc tgaagaagaa cgtgggaact
       61 taattggtca aacgattgaa gttgccccca ttcgcattga ttatggacgc attcgtttaa
      121 tcgtttctca aaatgttgct aatgctatta aaaagcgaaa tattaaaaaa gaattcctag
      181 aacaaattca agttggtcaa atttttgaag gcactgttaa aaatgttgaa acttatggag
      241 cttttgtgga attaggcaat ggtgttgaag gcttattaca tatttctgaa tacgaccatg
      301 taagaatatt caaacttgac cgtgttgtaa aaccgggtga taaagttcaa gtgcaagtca
      361 taaaaattga caatgaacat gttggcttat ccagaaaagc tttaattcct aatttctggc
      421 aagaatacac cgaaagcaaa gaagttgggc aagtaattaa tggcaaagtc attgaaatca
      481 ataatgccgg tttagtcatg gagttgagtc ctgaagtaac agggttttta ccaaaaagtg
      541 aatttgccta tgaacgcgat gtttacatta gtgatacggt taatgttggc gaccaaattg
      601 atgctaaaat tattgaaatt gatcctaata aacgtcgtat tatcttatct aaaaaacaat
      661 taaccgctaa cccgtgggat gatttaaaga ttaaagcggg cgacaatgtt gaagttttag
      721 tcacaaaaga actaaaagac ggtttcaaag ttgaattaaa tggggcgact ggttatttac
      781 caaagagcaa tatttttaat tatccagttg aagatatcgt tgctggtagt caattaaaag
      841 ttaaagtccg tgtttttgat ccggagaaaa cccgcttggt tgtcggctta aaagttgaaa
      901 cggaacgaat tgaacgagaa tcttacaata aatttatgaa agcacaagac aaactaacta
      961 acaattttgg cgatttatta gcgaataaat ttaaagattc tcataagtag gaagcatatg
     1021 cgaggagtag ttgctattgt gggccgacca aatgtcggta aatccagttt atttaatcga
     1081 attgtgggcg aaagattatc catcactgat gacgcaagcg gaataactag agaccgaatt
     1141 tatagcaaa
//
LOCUS       DAOSGN010000370         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_133251_length_1146_cov_2.076994, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000370 DAOSGN010000000
VERSION     DAOSGN010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133251_length_1146_cov_2.076994"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PK087_04940"
     CDS             <1..593
                     /locus_tag="PK087_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOH18649.1"
                     /translation="HALITGFDVMDPVVFFGLLVGSAVPAVFSALLMLGVNRNAERMV
                     GEIHRQFQEIPGLLEGKDGVSPEYDKCIAIATTGALRELIPAGLFAIVITILVGFVGG
                     VAAVGGFLVGNIVSGLLLALFMSNSGGLWDNGKKYVEAGNFGGKGSPAHKAAVVGDTV
                     GDPFKDTAGPSINTQITVVSLLASLLSTIFLTLSIF"
     gene            825..>1146
                     /locus_tag="PK087_04945"
     CDS             825..>1146
                     /locus_tag="PK087_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18650.1"
                     /translation="MVATLIISLLTIILLVLAIMFKQSIKIRNHYFETFWMVAVFGVV
                     LLLLFGRISFKEIFLGITEASSINPLKILVLFLSMTILSVILDELGFFKKLAYLALKK
                     AQGKQ"
BASE COUNT          305 a          177 c          259 g          405 t
ORIGIN      
        1 gtcatgcttt aattaccggt tttgatgtta tggatccggt ggttttcttc ggtctcttgg
       61 tcggatcagc tgttccagcc gtcttcagtg ctttgttaat gctcggtgtt aaccgcaatg
      121 cggaacgaat ggttggcgaa attcatcgtc aattccaaga aattccgggt ctattagaag
      181 gaaaagatgg tgtttctccg gaatatgaca aatgtatcgc tattgcgaca acgggcgctt
      241 tacgggaatt aattccggcc ggattgtttg cgattgtcat tacgattttg gttggttttg
      301 ttggtggtgt ggctgccgtg ggcggtttct tagttggtaa tatcgtcagc ggtttattgc
      361 ttgccctctt tatgagtaat agtggtggtt tatgggataa cggtaaaaaa tatgtggaag
      421 ccggcaattt tggtggcaag ggaagtccgg ctcacaaagc tgctgttgtt ggtgataccg
      481 ttggtgatcc atttaaggat actgcgggcc caagcatcaa tacccaaatt accgtggtta
      541 gtttactggc cagcttatta agcacgattt tcttaacctt atcgattttc taaacatttt
      601 aatataaaaa tgaaagtctc cattagtgat atgatggaga cttttattta gcggaaaaaa
      661 ttagatttta ttggctgctt cgacttacaa aagaccaaaa aaagggtctt ttttgtttat
      721 gagaacaatg aaaataactg cagggatttt gtcttgaaaa aaatcatgat aagttgtata
      781 atttgttatc atttaccaag gatttgttta gaaaaggata aaatatggtc gcgacgttaa
      841 ttatttccct attaacgatt atcttattag tcttagcaat catgtttaaa caaagcatca
      901 aaattcgtaa tcattatttt gaaacatttt ggatggttgc ggttttcggg gttgtgcttt
      961 tgttgctgtt tggccggatt tcttttaaag aaatcttttt agggattacg gaagcttcct
     1021 ccattaatcc gctgaaaata ttggtattgt ttttatccat gacgatttta tccgttattc
     1081 ttgatgaact gggctttttt aaaaaactgg cttatttggc tttaaagaaa gctcaaggaa
     1141 agcaaa
//
LOCUS       DAOSGN010000371         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_133540_length_1144_cov_3.456382, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000371 DAOSGN010000000
VERSION     DAOSGN010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133540_length_1144_cov_3.456382"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..661
                     /locus_tag="PK087_04950"
     CDS             23..661
                     /locus_tag="PK087_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOH18651.1"
                     /translation="MFSWNLLYIVPVALIAIMVHEVSHGLVSTWLGDPTPKAQGRLSF
                     NIFKHIDVFGTLSLIFFGFGWAKPVEIDPRYYKNIKTGTALVAIAGPISNFLLAFISA
                     FVYLLIFKILGWDLSGFALIVSNFFYYLTIINLGLGIFNLFPFPPLDGSKVLAVVLPD
                     QWYRKLMYYERIGMFLLVILIVLNVLDRPLNWLITNTLELLYNLVAFILNIN"
     gene            675..>1144
                     /locus_tag="PK087_04955"
     CDS             675..>1144
                     /locus_tag="PK087_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOH18652.1"
                     /translation="MNQCTETTIDISGETYPVSIIRKNYSRLSLKIRFSLRVFVSCPN
                     HVSDEEAIAFMHANMDWLEINMSKIQWKLSLFPLREMMAGKKILWFGEVLPVVKNEEL
                     SVPFKFFNGKLYVPESYYESLREIENYAKSIVTEKYQIILSKMVSDTQSVLPLS"
BASE COUNT          321 a          179 c          213 g          431 t
ORIGIN      
        1 taattcttaa aaaggattat ttatgtttag ttggaattta ttatatatcg ttccagtggc
       61 attaattgcc atcatggttc atgaagtttc tcatggcctt gtttctactt ggctgggaga
      121 cccaaccccc aaagcccaag ggcggctttc ttttaatatt tttaaacaca ttgatgtttt
      181 tggaactctc agtttaattt tttttggttt tggatgggcg aaaccggtag agattgatcc
      241 gcgttactac aaaaatatca aaacaggaac ggctttggta gcgattgcgg gtcctatcag
      301 taattttctt ttagccttta ttagtgcctt tgtttatttg ttaattttta aaattcttgg
      361 ttgggattta agcggtttcg ctctaatcgt tagtaatttc ttttattatt taacgataat
      421 caatcttggt ttaggaattt ttaatttatt tccctttccc cctttggatg gatcgaaagt
      481 tttggccgtt gttttacccg accaatggta tcgaaaacta atgtactatg aaagaattgg
      541 gatgtttttg ttagttattt taattgttct taatgttttg gatagacctc ttaattggtt
      601 aataaccaac accttagaac ttctatataa tttggtcgca ttcatattaa atattaacta
      661 aaggagatgt cttgatgaac caatgtactg aaacaacgat tgatattagc ggtgaaacct
      721 atccggtttc tattattaga aaaaattatt cgcgtctttc cttaaaaatt cggttttcat
      781 tgcgggtttt cgtctcttgc cccaaccatg tgagtgatga agaagcaatt gcattcatgc
      841 atgccaatat ggattggtta gaaattaata tgtccaaaat ccaatggaaa ttatcgcttt
      901 ttcctttgcg ggaaatgatg gctggtaaaa aaatcctttg gtttggggaa gtgcttccgg
      961 ttgtgaaaaa cgaagagtta agcgtccctt tcaaattttt taatggtaaa ttatatgttc
     1021 ctgagtcata ttatgaatcg cttcgagaaa ttgaaaatta tgcaaagagt attgttacgg
     1081 aaaaatacca aattatcctt tccaaaatgg ttagtgacac tcaatcagtt ttgcccttgt
     1141 catt
//
LOCUS       DAOSGN010000372         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_133765_length_1143_cov_2.604779, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000372 DAOSGN010000000
VERSION     DAOSGN010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133765_length_1143_cov_2.604779"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /gene="xseA"
                     /locus_tag="PK087_04960"
     CDS             <1..1090
                     /gene="xseA"
                     /locus_tag="PK087_04960"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOH18653.1"
                     /translation="HKNGTYNLTVSEMSQAGLGESYLNFLRLKEKLQKEGLFDQDKKL
                     PIPAFVEHIAVITSETGDAINDITSTIFKRFPLTKVYLYPALVQGSQAPKSLIAALNK
                     ANQDRLAQVIIIARGGGSVEDLSCFNDEELARAIFASKIPTVSGVGHEADYTICDFVA
                     SLRAPTPTGAAVLLTRSQSEIAQNINEKTQLLNYYYKRILEQKYFVFHNLVNTYHFRN
                     FVETIKAKENYLNQLETKLKASSPEAKIVNLLTRIDNVNQRLLAYNFPSKIEQKMVLL
                     ADKLAKMTQIWQTSINDRMNYTALLTEKLDLLNPLNLMKKGYTLTYQQNKLIMKASDV
                     QLEKALKVIYYDGEVETMPVKKNQRKEG"
BASE COUNT          404 a          189 c          211 g          339 t
ORIGIN      
        1 tcataaaaat ggaacataca atttaacggt tagtgaaatg tcgcaagccg gccttggtga
       61 aagttactta aattttttac gtttaaaaga aaaattacaa aaagaaggtt tattcgacca
      121 agataagaaa ttacccattc cggcttttgt tgaacatatt gcggtcatta cttccgagac
      181 tggtgatgcg attaatgata taacttccac catttttaag cggtttccgt taactaaagt
      241 ctatttatat ccggctcttg ttcaaggttc tcaagcaccg aaatcattga tagccgcttt
      301 aaataaagct aaccaagatc gcttagccca agttattatt attgcgcgcg gtggtgggag
      361 cgttgaggac ttatcatgtt tcaatgatga agagctcgct agagcgattt ttgctagtaa
      421 aattcctact gttagtggtg tcggccatga agctgattac acgatttgtg attttgttgc
      481 ttcccttcgg gctccaaccc ctacgggagc agccgttttg cttaccagga gtcagagtga
      541 aattgctcaa aatattaacg aaaaaacaca attgttaaac tattattata aacggattct
      601 tgaacaaaaa tattttgttt ttcataacct tgtaaatacc taccatttcc gtaattttgt
      661 ggagacaatc aaggcaaaag aaaattactt aaatcaactg gaaactaaat taaaagcttc
      721 ctcaccggag gctaagattg ttaatttatt aacgcgtatc gataatgtaa accaacgctt
      781 acttgcttat aattttccca gcaaaattga acaaaaaatg gttttacttg ctgataaact
      841 ggcaaagatg acacaaatat ggcaaacatc gattaatgac cgcatgaatt atacggcttt
      901 actaacggaa aaactggatt tattaaatcc cttgaatttg atgaaaaaag gatatacttt
      961 aacatatcaa cagaataaat tgattatgaa agctagcgat gttcaattag aaaaagcatt
     1021 aaaagtaatc tattatgacg gtgaagtgga aacgatgccc gtcaaaaaga accaacggaa
     1081 agaaggataa aagatgtacg aagaattaat taaagaatta gaagagattg taaaagcttt
     1141 gga
//
LOCUS       DAOSGN010000373         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_134284_length_1140_cov_3.276498, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000373 DAOSGN010000000
VERSION     DAOSGN010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134284_length_1140_cov_3.276498"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1140
                     /locus_tag="PK087_04965"
     CDS             <1..>1140
                     /locus_tag="PK087_04965"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317988.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HOH18654.1"
                     /translation="IGYIKQIDNDEVYITLETEFVGMCPDANEDGIIRRKLNEVDKPL
                     EIFYEQICHRVANNLAQGEKLETVRDFYHELHSLNLIPAGRVLFGAGSNSAVTYFNCF
                     VMPFVTDSRGGISIHRQKVMEIMSRGGGVGTNGSSLRPKNSLAKGVSGKSSGAVSWLH
                     DLSELTHLVEQGGSRRGAQMIMLADWHPDIIEFIISKMQNPRILQFLINNLSDEDIIK
                     EAKNKLKFIPLTAEQQEMYEWIVESANQNPTAISKATVEKAKEVLLAGGFYEVNNPNF
                     LSGANISVAITKEFMNAVENDLEYSLRYPDIDNYSLGEKAAYDEKWHNVGDVRIWEKM
                     GYAIKTHKVIRAKELWNLINICATYSAEPGIFFIDKANDKTNAKGY"
BASE COUNT          351 a          236 c          249 g          304 t
ORIGIN      
        1 attggttaca ttaagcaaat tgacaacgac gaagtttata ttactttgga aacggaattt
       61 gtggggatgt gccccgatgc taacgaagat ggcatcatcc gccgcaaact gaacgaagtt
      121 gataaaccat tagagatttt ctatgaacaa atttgccacc gcgttgccaa caatttagct
      181 caaggtgaaa aacttgaaac tgtccgtgat ttctatcacg aattacatag tttaaattta
      241 atcccggccg gccgcgtctt atttggggcc ggttccaatt ccgccgttac ttatttcaat
      301 tgttttgtta tgccctttgt gaccgattca cgcggcggca tttccatcca tcgccaaaag
      361 gtaatggaaa tcatgtcccg tgggggtggt gtcggcacca acggatcatc cttgcgtccg
      421 aaaaactccc ttgcgaaagg agtaagcggt aaatcaagcg gggcggtcag ttggcttcac
      481 gacttatccg aactcaccca tctcgttgaa caaggtggct cccgaagagg ggcgcagatg
      541 attatgttag cggattggca tccggatatc attgagttca tcatcagcaa aatgcaaaat
      601 ccacggattt tacaattcct catcaataat ttaagcgatg aagatatcat caaagaagcc
      661 aaaaataaat taaaatttat tccgctgacg gctgaacaac aagaaatgta tgaatggatt
      721 gtggaaagtg cgaaccaaaa cccaactgcc atcagtaaag caacagtaga aaaagccaaa
      781 gaggtcttat tagccggtgg cttttatgaa gtcaataacc cgaacttttt gtcgggagcc
      841 aatatttccg tcgccattac caaagaattt atgaatgcgg tggaaaatga tctcgaatat
      901 tccttacgct atccggacat tgataattat tccctaggtg aaaaagccgc ttacgatgaa
      961 aaatggcata atgttggtga tgtgcgcatt tgggaaaaaa tgggctatgc cattaaaacc
     1021 cataaagtca tccgcgccaa agagctttgg aacttaatta atatttgtgc cacttattcg
     1081 gcggaaccgg gtattttctt tattgataaa gccaacgata agactaatgc gaaaggctat
//
LOCUS       DAOSGN010000374         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_134627_length_1138_cov_3.870729, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000374 DAOSGN010000000
VERSION     DAOSGN010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134627_length_1138_cov_3.870729"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PK087_04970"
     CDS             complement(<1..780)
                     /locus_tag="PK087_04970"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOH18655.1"
                     /translation="MKIFDVHSDLLYDLYTKKKEGKVNRFALYHAPQLRISDIKGAIW
                     TMYSPDDFDLVESIEIALGEIDIHLLPDFQVLLGLEGLRNLPKATDLKKVYDLGIRHA
                     MLTWNEENQYATGVAGDKNRGLMPEGKTLLSMMEELDMIIDVAHLNEKSFYDVLNYTQ
                     KNIIFSHGNVKSLCEHRRNLSDEQLKALRSVDGLFGLTLANNFVSPNKQEQNLDHLLN
                     HLEAAIDIMDIDHIMFGFDFMDYLSEFPNANLIDLPNASQTK"
     gene            complement(782..>1138)
                     /locus_tag="PK087_04975"
     CDS             complement(782..>1138)
                     /locus_tag="PK087_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOH18656.1"
                     /translation="FIDGGMADNIRPALYQAKYTVGVANRFNQPLSNQALSYDIVGKC
                     CESGDVIAKDVVLEQVEEGDTIIVFATGAYCYSMSMNYNGLTRGEVVFIKDDQIKVVI
                     EKESLDQLVSTCVFGG"
BASE COUNT          344 a          248 c          203 g          343 t
ORIGIN      
        1 tttagtttgg gaagcattcg gcaaatctat taaattggca ttcggaaatt cgcttaaata
       61 atccataaag tcaaaaccga acataatatg atcgatatcc atgatgtcga tggcggcttc
      121 caagtgatta agcaaatgat caagattttg ttcttgtttg tttggtgaaa caaaattatt
      181 cgctaaggtt aggccaaata aaccatccac actccgcaag gcttttaact gctcatcgct
      241 taaattgcgc cggtgctcgc acaaactttt cacatttcca tgggaaaaga tgatgttttt
      301 ttgcgtatag ttaaggacat cataaaaact tttttcattg aggtgagcca catcaataat
      361 catatcgagc tcttccatca tggaaagtaa ggttttccct tccggcatga gaccacggtt
      421 tttatccccg gccacccctg tcgcgtactg gttttcttcg ttccaagtga gcattgcatg
      481 gcgaattcct aaatcataca cttttttaag gtcggtagcc tttggtaaat tgcgcagtcc
      541 ttccagtccc aacagcactt gaaaatcagg aagcaagtga atatcaattt ctccaagagc
      601 aatttcaatg gactccacaa gatcaaaatc atcgggggaa tacatcgtcc aaatagctcc
      661 cttgatatca gaaattcgta gttgcggggc atggtaaagg gcaaaacggt ttacttttcc
      721 ttcctttttt ttagtatata aatcatagag taaatcacta tgaacatcaa agattttcat
      781 gttagcctcc aaaaacacat gtggaaacaa gctggtctaa ggactctttt tcaatcacca
      841 ctttaatttg atcgtcttta ataaagacga cttctcctct tgttaaaccg ttatagttca
      901 tcgacatcga ataacaataa gcaccggttg caaaaacaat aatcgtatca ccttcttcta
      961 cttgttctaa taccacatct ttcgcaatca catcgcctga ttcacaacat tttcccacaa
     1021 tatcataaga aagggcttga ttggataaag gctgattaaa gcggttggca acaccgaccg
     1081 tatatttagc ttgatagagg gctgggcgaa tattgtccgc catcccccca tcaatgaa
//
LOCUS       DAOSGN010000375         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_135921_length_1131_cov_2.791822, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000375 DAOSGN010000000
VERSION     DAOSGN010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135921_length_1131_cov_2.791822"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>1131
                     /locus_tag="PK087_04980"
     CDS             13..>1131
                     /locus_tag="PK087_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18657.1"
                     /translation="MEKATVTSNSYDFSALTGDKLLFQVVAKAPKGMADSPKSASITY
                     TAAIVLSAPVNLAFNGKVLTWDAVKQATEYIVYVNDVEKATVTTTSYDFSSLSGDNLV
                     FQVVAKAPKGMVNSPKSAALTYSTTAAEISAPVNLEISGKVLTWDAVKQATGYIIYVN
                     DVEKATVTTTSYNFSALTGDKLVFQVVAKAPKGMNNSAKSVMIAYIANPTAEITAVKN
                     LLDEIMPEVSDIAAEELVRKGMTGADVEALKAAVETLLADMEAADGDPVLSNAALKTF
                     LATKFNIEALVSTGLVLAGPGIDEEIANIEEEIDWYQNEIDQYGPSDYYQSRIDELTN
                     EKEMLINMKAMIASSRDEMVLVATKTVDYLITLQTKVTD"
BASE COUNT          345 a          207 c          257 g          322 t
ORIGIN      
        1 tatgtgaata atgtggaaaa agcaacggtt acaagtaatt cttatgattt ttccgcttta
       61 accggtgaca aattactttt ccaagtagtt gcgaaagccc cgaaagggat ggctgattcc
      121 cctaaaagtg cttcgattac ctatacggcc gctattgtct tgagtgcccc tgttaatctt
      181 gctttcaacg ggaaagtatt aacttgggat gccgttaaac aagctaccga gtacatcgtt
      241 tatgtaaatg atgttgaaaa agcaacagtc accaccacat cttatgattt ttcttctttg
      301 agtggagaca atttagtttt ccaagtggtt gcgaaagccc cgaaggggat ggttaactct
      361 ccaaagagtg ccgcgcttac ctatagtaca acggcagctg aaataagtgc tccggttaat
      421 ctagagattt ccggtaaagt gttaacttgg gatgcggtta aacaagccac tggatacatc
      481 atctatgtaa atgatgttga aaaagcgaca gttactacca catcttataa cttttccgct
      541 ttaactggcg acaaattagt tttccaagtg gttgcgaaag ccccgaaggg gatgaacaac
      601 tctgctaaaa gtgtaatgat tgcctatata gctaatccga cagcggagat tacggcggtt
      661 aaaaatcttc ttgatgaaat tatgccggag gtttcggata ttgctgcgga agaacttgtt
      721 cgcaaaggga tgaccggtgc ggatgtggaa gctcttaaag cagctgttga aaccttattg
      781 gcggatatgg aagccgccga tggtgatcca gtcttatcca atgccgcctt aaagacattt
      841 ttagcgacca agtttaatat tgaagccctt gtcagtacag gattagtgtt ggctggcccc
      901 ggcattgatg aagaaattgc caacattgaa gaagagattg attggtatca aaatgagatt
      961 gatcaatatg gtccaagtga ttattatcaa agccgaatag atgaattaac aaatgaaaaa
     1021 gaaatgttaa tcaacatgaa ggcaatgatt gcgtcttcgc gtgatgaaat ggtcttagtt
     1081 gccacgaaga cggttgatta tttaattact ttacaaacaa aagttaccga t
//
LOCUS       DAOSGN010000376         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_136868_length_1126_cov_2.488329, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000376 DAOSGN010000000
VERSION     DAOSGN010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136868_length_1126_cov_2.488329"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PK087_04985"
     CDS             complement(<1..458)
                     /locus_tag="PK087_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HOH18658.1"
                     /translation="MAQETKKARLCSFCGLPESPERHLIEGDNAFICDVCVGVCHQMF
                     NLSLDEEEKSAPKTTDFALNLTPREIYERLSEYVIGQQYAKKVLSVAVYNHYKRVQNN
                     LFEKSEIDIDKSNILLLGATGSGKTLFARTLAKILNVPFAIADATTLTQAG"
     gene            complement(477..>1126)
                     /locus_tag="PK087_04990"
     CDS             complement(477..>1126)
                     /locus_tag="PK087_04990"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18659.1"
                     /translation="ERDLKVKFPDDYQAENLKGKEAVFKVKLHEIKKRVLPELTDEFV
                     VSLNIEPKTVNDYRTYQSDKIKTEKTEASDNKFEHDLLKKVCDDAKVNVPDVLVNRRV
                     DSMLKQEEDRAKAYGLTFEQLLKYQGMTKEQYQEMIKAPARFDVLKELVINKIIEQEQ
                     LSLSDEDYEYHYQEFAKMSNQDVEAVKKQYPKNQVAYHFLMLKTIEEMKKTAIIK"
BASE COUNT          298 a          236 c          196 g          396 t
ORIGIN      
        1 ccggcttgag ttagggttgt agcgtcggca atcgcaaagg gaacatttaa aattttggct
       61 aaagttctag caaagagcgt tttcccgctt ccggttgctc ccagcaataa gatattactc
      121 ttatcaatgt caatttcgct tttttcaaac aaattatttt gtactcgttt atagtgattg
      181 taaacggcca cggacagcac ttttttcgca tattgttgac cgataacata ttcgcttaag
      241 cgttcataaa tttcccgagg agtcaaattt aaagcaaaat cggtggtttt gggggccgat
      301 ttttcttctt catcaaggct caaattaaac atttgatggc atacaccaac acaaacatcg
      361 caaataaaag cattgtcacc ttcaattaag tgccgttccg gactctccgg caacccgcag
      421 aatgaacaaa gacgggcttt tttggtttct tgggccatgc ttacacctca attcatttat
      481 ttaatgatgg ctgttttctt catttcttca atcgttttta acattaagaa atggtaagca
      541 acctgattct tcggatattg ttttttaacg gcttccacat cctgattgga cattttcgca
      601 aattcttgat aatgatattc gtaatcctca tcacttaggc tcaactgttc ttgttcaata
      661 atcttattaa taactaattc tttgaggaca tcaaaacgcg ccggagcttt aatcatttcc
      721 tgatattgtt ctttggtcat accttggtat ttcagcagtt gttcaaaggt aagcccataa
      781 gctttggctc ggtcttcttc ttgtttcagc atgctgtcaa cccgacgatt tactaacaca
      841 tccggcacat tgactttagc atcatcgcag acttttttca acaaatcatg ttcaaacttg
      901 ttatcggaag cttcagtttt ttccgttttg attttgtcgc tttgataagt gcgataatca
      961 ttaacggttt tgggctcaat gtttaaggaa acaacaaatt catcggtcaa ctccggtaaa
     1021 acgcgttttt taatttcgtg cagtttaact ttaaaaacgg cttccttgcc ctttaagttt
     1081 tccgcttgat aatcatccgg gaatttaacc tttaaatcgc gttctt
//
LOCUS       DAOSGN010000377         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_137334_length_1124_cov_1.668849, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000377 DAOSGN010000000
VERSION     DAOSGN010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137334_length_1124_cov_1.668849"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1124)
                     /locus_tag="PK087_04995"
     CDS             complement(<1..>1124)
                     /locus_tag="PK087_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18660.1"
                     /translation="GFSAFSLYQELTITVYSLETAEEIAINQLAQTILASIPNETMQD
                     LNLITYDETSKATITYQSNQEEALTNLGKITRTTDDVRVKLKITIKINDTSGFFEKTV
                     VVRKLTALNLTGDFTLTLGEISVVSASVESSRLVYSSSNPIIFTVDEVGKITALAPGK
                     AVLTVALSDDLSSFVTHEITVVDPLNEIGAYLRDAIPDTIENDVVFLYQHPDYDINVS
                     YQTNSLYIDNEGKVTKDAENRQVTINIHVTLGNRSKTFYKSAVVTMIAVETQLANTRA
                     WFKNLALVSNEEGLLPEVDGVYGRSIFWIGSDPGMIINQHLFPSLEAKTVKLQAFFTI
                     AHRKYLYEYEYISKGVNPMEKLSALKRFMDGYFPSIAGKR"
BASE COUNT          355 a          233 c          198 g          338 t
ORIGIN      
        1 ggcgctttcc ggcaatgctg gggaaatagc catccataaa ccgctttaaa gctgataatt
       61 tttccatggg attaactcct tttgatatat actcatattc atagagatat ttgcgatggg
      121 cgattgtgaa aaaggcttgc agtttaactg tttttgcttc caaactaggg aacaaatgct
      181 ggttaataat catccccgga tcgctcccaa tccaaaaaat gcttcgcccg taaaccccat
      241 ccacctccgg aagcaatcct tcctcattgg aaacaagggc caaattctta aaccaagctc
      301 gggtattagc cagctgtgtt tcgaccgcaa tcatggtgac gaccgccgat ttatagaaag
      361 ttttactgcg attaccaagg gtaacatgaa tattaatcgt cacttgacgg ttctccgcat
      421 ccttggtaac tttaccctca ttatcaatat ataaagaatt ggtttgataa ctgacattaa
      481 tatcataatc cggatgttga tataaaaaca caacatcatt ttcaatggta tcaggaatgg
      541 catcccgtaa ataagcacca atctcattaa gaggatcaac aacggtgatt tcatgagtta
      601 cgaacgatga caaatcatcg cttaaagcga cagttaacac cgctttgcca ggagccaaag
      661 ccgtgatttt accaacttca tcaaccgtaa aaattatcgg attggatgaa gaataaacca
      721 accttgaaga ctccaccgat gcactcacaa cagaaatttc tcctaatgtt aaagtaaagt
      781 ccccagtcaa attaagtgcg gttaatttac gaacaacaac tgttttttca aaaaaaccgc
      841 tcgtatcatt aatcttaata gttattttaa gtttaaccct aacatcatcc gtagttcttg
      901 taattttccc taaatttgta agagcttctt cttgattgga ttgataagtg attgttgcct
      961 tactagtttc atcataagtt attaagttaa ggtcctgcat cgtttcatta ggaatactag
     1021 ctaggatggt ttgcgcaagc tgattaatag caatttcttc tgctgtttct aaagaatata
     1081 cggtaatcgt taattcctga tagagagaga atgctgaaaa gcca
//
LOCUS       DAOSGN010000378         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_137873_length_1121_cov_1.986867, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000378 DAOSGN010000000
VERSION     DAOSGN010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137873_length_1121_cov_1.986867"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PK087_05000"
     CDS             complement(<1..612)
                     /locus_tag="PK087_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH18661.1"
                     /translation="MDYASVRNRLIRAAAKTKTPMVGAFELTPSCNLACKMCYVRESF
                     PKKELSTAEWKTLILEAKNAGLLFCLFTGGEIFLRSDFQDIYEFTYDLGMRISLFTNG
                     TLISDEVVNYLKKRKPDLVAITLYGINPHQYQEITGSAQAFSQVSSAIKRLKEAKITV
                     VVRTIAVQTIYRHLDDVINYLKELHLPVSYSLYVGPKRSCLGSS"
     gene            complement(629..892)
                     /locus_tag="PK087_05005"
     CDS             complement(629..892)
                     /locus_tag="PK087_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOH18662.1"
                     /translation="MKVKQGFMLREIANNYVVVPVGKAAIDFNGIITLNQTGAFLWEQ
                     LQEEISLASLQQKLMAEYEVDEKTALADIKDFIDHLKTNNLLE"
     gene            complement(889..>1121)
                     /locus_tag="PK087_05010"
     CDS             complement(889..>1121)
                     /locus_tag="PK087_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18663.1"
                     /translation="KWKYLWKRAFPPYKTMKLLYPSLTKNAIFLPFYYLHRLFKGFLT
                     GKVATKTKQLRAVRKEDVQLNQNVKQKTGVQK"
BASE COUNT          348 a          227 c          196 g          350 t
ORIGIN      
        1 agaacttccc aaacaagagc gtttaggccc aacatataag ctgtaagaaa ccggcaagtg
       61 gagctctttt aaatagttga taacatcatc gagatggcga taaatcgttt gaacggcaat
      121 cgtcctaaca actaccgtta tcttcgcttc ctttaagcgt ttaatagcgg atgaaacttg
      181 tgaaaaggct tgggcagaac cggttatttc ttgatattga tggggattaa tgccatataa
      241 agtaatcgct accaaatcgg gtttccgctt ttttaaataa ttaacaactt catcactaat
      301 caaggtccca ttggtaaaaa gggaaatcct cattcctaaa tcataagtaa attcatagat
      361 gtcttgaaaa tcagatctta aaaagatttc cccacctgta aacaagcaaa aaaggaggcc
      421 cgcattttta gcctctaaaa tcaaagtttt ccattcagcg gtcgaaagct cttttttagg
      481 aaaactttcc ctcacataac acatcttgca agctaaattg caggaaggtg ttaactcgaa
      541 agcccccacc attggtgttt tcgttttagc agccgctcta ataagccggt tgcgaacgct
      601 ggcataatcc attaagcctc ctcgttagct attccaacaa attattagtt ttcaaatgat
      661 caataaaatc tttgatatca gctaaagccg ttttttcatc aacctcatat tcagccatca
      721 atttttgttg caaagaagcc aaagatattt cttcttgcaa ttgttcccac aaaaaagcac
      781 cggtttggtt caaagtaata atgccattaa aatcaatggc ggctttacca accggaacca
      841 caacataatt gttggcgatt tctcttaaca taaatccttg ttttactttc atttttgtac
      901 tcctgttttc tgcttgacat tttgatttaa ttggacatct tcttttcgaa ctgctcttag
      961 ttgtttggtt ttggtcgcta cttttcccgt tagaaagcct ttaaataaac gatgaagata
     1021 ataaaacgga aggaagatag cgtttttggt gagagaggga tacaaaagct tcatggtttt
     1081 atagggagga aaggcccgct tccaaagata tttccactta c
//
LOCUS       DAOSGN010000379         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_138389_length_1118_cov_2.492004, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000379 DAOSGN010000000
VERSION     DAOSGN010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138389_length_1118_cov_2.492004"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PK087_05015"
     CDS             complement(<1..943)
                     /locus_tag="PK087_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH18664.1"
                     /translation="MKRLIIHHIRQLVTVSGPNRLRRGAEMGDLKIIENGFLVVEAGK
                     IIALGSNRDYLAFVNTEQCELINGEDFIVLPGFIDSHTHLVHYGSRENDLERQAKGES
                     YLDILKSGGGIHQTVSQTQNASFSQLYEKAKASLKTMLAFGVTTVEAKSGYGLDFASE
                     KKQLEVGKALNKELGPLVVNTFMGAHAFPKTCEHRDTYLSNLLKWMEVFRKEELAEFV
                     DVFCEEGVFSLAETKLILERAQALGYGLKMHADEMVSLGGTSLAVSLNCHSVDHLLVT
                     KDEDIDLLATSTTVATILPLTSFYLNKPMARARFMIDK"
BASE COUNT          323 a          263 c          201 g          331 t
ORIGIN      
        1 ctttatcaat cataaagcga gctctagcca tcggtttatt taaataaaac gatgttaaag
       61 gtaaaattgt tgccacggtt gtgcttgtcg ctaacaggtc aatatcctca tccttagtca
      121 ccaataaatg gtcaacggag tgacaattta aggaaactgc taagctcgtc ccccctagtg
      181 aaaccatttc atcggcatgc atttttaaac cgtaacccaa ggcttgtgct ctttctaaaa
      241 taagtttagt ttccgcaagc gaaaaaacgc cctcttcaca aaagacatcc acaaattccg
      301 caagctcctc ctttcggaaa acttccatcc atttaaggag attagaaaga tatgtatcgc
      361 gatgttcaca agttttcgga aaggcatgag cccccataaa ggtattaacc acgagcggtc
      421 ccaattcctt atttaaggct ttccctactt ccagttgttt tttttcgctt gcgaaatcaa
      481 gcccataacc gcttttagct tccacggtcg tcacgccaaa agccagcatc gtttttaaac
      541 tggctttggc tttttcgtat aattgggaaa aactggcatt ttgcgtttgt gaaacggttt
      601 ggtgaatgcc accgccgctt tttaaaatat ctaaataact ttccccttta gcttggcgct
      661 ctaaatcatt ttcccgtgaa ccataatgaa ctaaatgggt gtgggaatca atgaaaccgg
      721 gaagcacaat gaagtcttct ccgttaatta attcgcattg ttctgtgttt acaaaggcta
      781 aataatccct attactgcca agagcaataa tcttccccgc ttccacaacc aaaaaaccat
      841 tttcaataat tttaaggtcc cccatttccg caccacgacg taaacgatta ggcccggaaa
      901 cggtcactaa ctggcgaata tggtgaatga tgaggcgttt catgatgatt gttttatcct
      961 atcccgcagt gtttttaata acttatcatc gaccaaataa ggaattgtca aatgatcatg
     1021 attattttgg ttatgcataa tagccgtttc gatggcatga ggattgcgcg cccaagcacg
     1081 tcttcccaca ccgaccatga catcccaact aatggctt
//
LOCUS       DAOSGN010000380         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_139108_length_1114_cov_2.735600, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000380 DAOSGN010000000
VERSION     DAOSGN010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139108_length_1114_cov_2.735600"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1114)
                     /locus_tag="PK087_05020"
     CDS             complement(<1..>1114)
                     /locus_tag="PK087_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH18665.1"
                     /translation="DITELLDKHAPNITGSVSEEVWKTATFDGKIYGVPQKNPSNNIN
                     SALFLRKDIFTKNQVALPTTLTEFKEALVALKTKLGSSFVPFSASSHNIEPIRGAFGI
                     VTDWVPQSDGKLKYWTETPAFQDYANYMKELVNLGVMQDGYLTTPNYGANSRAGLQNG
                     TVGVTLDAWWSGNGIINGIAASEKQTEEYVIAHAGDYLHFIDSLLGDANYGTPGKGQA
                     KMDYSVTYFYAIPKYMNKTAVAVIKWMNQKMDPVNFKRLTIGEENVHHKVVDGKYFPI
                     LTPDAKGEIPFNSFNKGDYFLTGIREEDYSLYWQCRARKNPRQQFVWEGMNTAAQKAS
                     VGIYDRVGLAPGFLIYGANKQKLAQFTTEYLVSLITG"
BASE COUNT          320 a          227 c          204 g          363 t
ORIGIN      
        1 gaccggtaat tagacttact aagtattcag tcgtaaattg tgccaatttt tgtttgttag
       61 caccataaat taagaaaccc ggagctagac caacacggtc ataaattcca acactggctt
      121 tttgggccgc cgtattcata ccttcccaaa caaattgttg acgaggattt ttacgagctc
      181 ggcattgcca gtataatgaa tagtcttctt cacgaattcc tgttaagaaa taatcaccct
      241 tgttgaacga attaaacgga atttcacctt tggcatccgg tgttaaaatc gggaaatatt
      301 ttccatcaac gactttgtga tgaacatttt cttcaccaat tgttaaacgt ttgaagttaa
      361 ctggatccat tttttgattc atccatttaa ttacagctac tgcggtttta ttcatatatt
      421 taggaatagc atagaaataa gtaacggaat agtccatttt ggcttgacct tttcctggtg
      481 ttccatagtt ggcatcacct aataaggaat cgataaaatg taagtaatcg ccagcatgag
      541 caattacata ttcttctgtt tgcttttcag aagcagcgat accgttaata atgccattcc
      601 cgctccacca tgcgtcgagg gtaacaccga cagttccgtt ttgcaatcct gcccggctgt
      661 tggcgccata attaggagtt gttaagtaac catcttgcat aacacccaaa ttaaccaatt
      721 ctttcatata attagcgtaa tcttggaaag caggagtttc ggtccaatat tttaatttac
      781 catcgctttg gggaacccaa tccgtaacaa ttccaaatgc tcctctaatt ggttcaatat
      841 tatgcgaact agcgctgaat ggaacaaaac ttgagccaag tttagttttc aaagccacta
      901 aagcttcttt gaactccgtt aatgtggttg gaagagcaac ttgattcttt gtgaagatgt
      961 cttttcttaa gaataaggca ctgttgatgt tgtttgatgg attcttttga ggaacaccat
     1021 aaattttgcc atcaaaagta gccgttttcc atacttcttc actgaccgat ccggtaatgt
     1081 tgggagcatg cttatctagc aattccgtaa tgtc
//
LOCUS       DAOSGN010000381         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_139313_length_1113_cov_2.437618, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000381 DAOSGN010000000
VERSION     DAOSGN010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139313_length_1113_cov_2.437618"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PK087_05025"
     CDS             complement(<1..315)
                     /locus_tag="PK087_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18666.1"
                     /translation="MFKKLLFIFLLIFSVFGLAACDGDDTPDVDKTESVDVPINLAIS
                     GKVLTWDAVEKATGYIVYVNDVEKKTVTTTSYDFSSLSGENLIFQVVAKAPKGMNNSA
                     KSV"
     gene            complement(325..939)
                     /locus_tag="PK087_05030"
     CDS             complement(325..939)
                     /locus_tag="PK087_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18667.1"
                     /translation="MVGFYKGYSEILAFTNLHLSDKYQEAPLVNTLFINSDDSHLQKD
                     LIKRYSNRMYFILDAQDLFLQEVERLVAVANFLTFIVWVLVGCFVLVIMNNSLLVFYS
                     LKADYAKIKILGCKDGELFVNILKEIIIIMALALVASFVNVLIVFALFPSLMIMVGYY
                     FQYQYHISDVLWYLLIGILVFSLSYVNYFFKVRNMNLVTETINL"
BASE COUNT          412 a          226 c          162 g          313 t
ORIGIN      
        1 cacactttta gcagagttgt tcatgccctt cggggctttc gcaaccactt ggaaaattaa
       61 attttctcca cttaaagatg aaaagtcata agaagtggtt gtgaccgtct tcttttccac
      121 atcattcaca taaacgatgt atccggtcgc tttttcaacc gcatcccaag ttaatacttt
      181 accggaaatt gctaagttga tgggaacatc aacactttcc gttttatcaa catcaggagt
      241 atcgtctccg tcacaggccg ccaagccaaa aacggagaaa atcaaaagga aaataaacaa
      301 taatttttta aacataaaaa cctcctataa attaattgtt tctgttacta agttcatatt
      361 tctcacttta aagaaataat taacataact tagtgaaaag actaaaattc cgattaataa
      421 ataccaaagc acatcactga tatggtattg gtattgaaaa taataaccaa ccataatcat
      481 cagtgacgga aacaacgcaa aaacaattaa gacatttaca aaactagcta ctaaagccag
      541 cgccatgata ataattatct ctttcaaaat attaacaaac aattccccgt ccttacatcc
      601 aagaatcttg attttcgcat aatccgcttt taaagaataa aagactaaca acgaattatt
      661 cataatcaca aggacgaaac agccaactaa cacccaaaca ataaaagtca agaaattagc
      721 aacagcgacc aaacgctcaa cttcttggag aaacaaatct tgggcatcca aaatgaaata
      781 catgcggttg ctatatcgct taattaaatc cttttgcaaa tgcgaatcat cgctattaat
      841 aaataaggtg ttaacaagcg gggcctcttg atatttatcg cttaaatgaa gattggtaaa
      901 ggcgagaatt tcactatagc ctttgtagaa accgaccacc tcaaattgtt cttcgggaat
      961 ggtccgacct aaagacagta ttactacatc gcctttttta attcgaccga ttttttcata
     1021 ctggtacggt aaaacaattg ctaagcgatt cgttaattgt aaatcattaa gactatcatc
     1081 aagaaattga atacaagtgc aactatgaga cta
//
LOCUS       DAOSGN010000382         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_139452_length_1112_cov_3.786187, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000382 DAOSGN010000000
VERSION     DAOSGN010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139452_length_1112_cov_3.786187"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PK087_05035"
     CDS             complement(<1..660)
                     /locus_tag="PK087_05035"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH18668.1"
                     /translation="MRLQFIIDKDDVVKNFLKRNHISKRLGRKIKWNGTIYINGLEAG
                     NWFSIHPGDVLEIHFDEEINSEIVASPWPVEVLFEDEYLLVVNKPANLASQPSHKYYE
                     DNLISRLKFYFEKNNINSNVHLVNRLDLSTSGLVLVAKNGFVHHLLMKTAIDKKYLAV
                     VEGKMPTASGVIALPIERDNHPIKRKVSAEGKHSVTEFRVIKSEDQSLLELTLRTGRC
                     HQ"
     gene            complement(697..>1112)
                     /gene="fusA"
                     /locus_tag="PK087_05040"
     CDS             complement(697..>1112)
                     /gene="fusA"
                     /locus_tag="PK087_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242251.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH18669.1"
                     /translation="FPVIGVKATLYYGSYHPVDSNEISFKLAAGLAFKNAVEKIRPTI
                     LEPICQVNVVVKDEFVGDIMGDMTKRRGRVLGMEQRNGVQEIIAEVPEAEITKYTVDL
                     KAMTQASGRFSRKFLRYEEVPEMLVKKIIEEYKKN"
BASE COUNT          338 a          242 c          189 g          343 t
ORIGIN      
        1 ttgatgacaa cgccccgttc gcaaggtcag ttccaataaa ctttggtctt cacttttgat
       61 aacccgaaat tccgtaactg aatgcttccc ttccgcactt acttttcttt tgatgggatg
      121 attatcgcgt tcaatcggta aagcaattac tcctgaagct gtcggcatct tcccttccac
      181 aacagctaag tattttttgt caatcgcggt tttcattaaa aggtggtgaa caaaaccgtt
      241 tttcgcaacc aaaaccaagc ccgaagtact caagtctaaa cgattgacta aatgaacatt
      301 gctgttgata ttgtttttct caaagtagaa ctttaaacga ctgattaaat tatcttcata
      361 atatttatga gagggctgac ttgctaagtt tgccggtttg ttcacaacta gtaaatattc
      421 atcttcaaaa aggacttcta caggccaagg tgaagcgacg atttccgaat taatttcttc
      481 atcaaaatgg atctctaaga catcaccagg atgaatgcta aaccaatttc cggcttctaa
      541 tccattaata taaatagtgc cgttccattt aatttttcga ccgaggcgct tagaaatatg
      601 atttcttttt aaaaagttct taacaacatc gtctttgtca atgataaatt gtaagcgcat
      661 agaaacccca acgagaaaag agtcgaataa tcgaccctaa ttctttttat attcttcaat
      721 gattttctta actagcattt caggaacttc ttcatagcgt aagaatttac ggctaaaacg
      781 accactggct tgggtcatag cttttaaatc gaccgtatat ttggtaattt ccgcttccgg
      841 aacttccgca ataatctctt gaacgccatt acgttgttcc ataccgagaa cacgaccacg
      901 acgtttggtc atatcaccca tgatatcacc aacaaattcg tctttcacaa ccacattgac
      961 ttggcaaatc ggttccaaaa tagtggggcg aattttctca accgcattct taaaagctaa
     1021 gccggcggct aatttaaagg aaatttcatt tgagtcaacc ggatgataag aaccatagta
     1081 aagggtggct ttcacaccaa tcacggggaa tc
//
LOCUS       DAOSGN010000383         1111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_139677_length_1111_cov_2.650568, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000383 DAOSGN010000000
VERSION     DAOSGN010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139677_length_1111_cov_2.650568"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..818)
                     /locus_tag="PK087_05045"
     CDS             complement(72..818)
                     /locus_tag="PK087_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007950705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOH18670.1"
                     /translation="MKMVSWNVNGLRAAMTKGFEDFFEKCNADFFAIQETKMQEEQKT
                     FAFPGYHEYWHSALKKGYSGTLIYTKQAPLSVTYGLDNAHMDEGRIITLEYPDFYFVT
                     SYSPNSQPELARLDYRMAFEDDFRAYLMKLDQHKPVIVCGDLNVAHREIDLKNPKANE
                     RNPGFSIEERTKFQALLDSGFTDSFRYFYPEKIKYTWWSYRANSRANNVGWRIDYFLV
                     SNRLASSLHDSYIHDEVMGSDHCPVSIEIF"
BASE COUNT          367 a          192 c          192 g          360 t
ORIGIN      
        1 aagaacacac tatgtttttg cttgcttttt taagtgttgg ttgaggttca aatcctcttt
       61 aggatttcat actaaaaaat ctcaatagaa accgggcaat ggtcacttcc catcacttca
      121 tcatgaatat aactatcatg taggctggaa gcaaggcggt ttgacaccaa aaagtaatca
      181 atacgccaac cgacattatt agcacgggaa ttggcccgat aactccacca agtatattta
      241 attttctccg gataaaaata acggaaagaa tcagtaaacc cactgtcaag aagagcttga
      301 aattttgttc gttcttcaat ggaaaaaccg ggattccgtt cattggcttt gggattcttt
      361 aaatcaattt cgcggtgggc gacatttaaa tcaccacaaa caatcaccgg tttatgttga
      421 tctaatttca ttaaataagc cctaaaatca tcttcaaatg ccatgcggta atcaaggcga
      481 gccaattcgg gttgtgagtt tggtgagtat gatgttacaa aataaaaatc aggatattct
      541 aaggtgatga tccgaccttc atccatatga gcattatcta aaccataagt tacgcttaaa
      601 ggtgcttgtt ttgtataaat caaagtgccg gaataacctt ttttaagagc ggaatgccaa
      661 tattcgtgat aaccgggaaa agcaaaagtt ttttgttctt cttgcatttt cgtttcttga
      721 atggcgaaaa aatccgcatt gcatttttca aaaaaatcct caaaaccttt agtcatcgcc
      781 gcccgtaagc cgttgacatt ccatgagacc attttcatat taattcctct tttctaattc
      841 ggtaaaccga tcatattatc ttgctttaaa acaaactaat tatttggtag ttttgcttga
      901 tgagaatcct aagttaaata atagtttata tcataactaa attgtaacac ataactaaag
      961 catcagcttt agtaatgtga cagttgttta agtgctttgt ttctttgggg ctattatatc
     1021 aagattaaag aagataagca aacaatgttc attgattgat agtgtcaaat tgttttgggt
     1081 aaaaataaat aaattaaaaa atggtgaaat t
//
LOCUS       DAOSGN010000384         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_140028_length_1109_cov_3.235294, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000384 DAOSGN010000000
VERSION     DAOSGN010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140028_length_1109_cov_3.235294"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PK087_05050"
     CDS             <1..401
                     /locus_tag="PK087_05050"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOH18671.1"
                     /translation="EKEKSDLELAILEVKDLHHEKIVIYNGTGGRLDHFIAILNVLIK
                     YSEANIEVVDEHNLMRIISQPTKLVKRDYKYFSLFALEEGTIISLQGFKYPLTNYHLA
                     IHDNLCLSNELVEDGFLEVNNKKILVIESK"
     gene            complement(442..633)
                     /gene="rpmB"
                     /locus_tag="PK087_05055"
     CDS             complement(442..633)
                     /gene="rpmB"
                     /locus_tag="PK087_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOH18672.1"
                     /translation="MPRKCYITGLGTVTGNNRPHSLVATRRSWKANLQKVRIVDTDGK
                     VKRVYVSAKALKSGLVQRA"
     gene            879..>1109
                     /locus_tag="PK087_05060"
     CDS             879..>1109
                     /locus_tag="PK087_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOH18673.1"
                     /translation="MEIFKLDGLLFKTLIVNGAENLKINYKQVDALNVFPVPDGDTGT
                     NMRMTLEGGINEIYNLDESNIGELAKRLQRGML"
BASE COUNT          362 a          165 c          210 g          372 t
ORIGIN      
        1 aggaaaaaga aaaaagcgat ttggaactcg ctattttaga ggtgaaagac cttcatcatg
       61 agaaaattgt tatttataac ggtacgggcg ggaggctgga tcatttcatc gccattctca
      121 atgtcttaat caaatattcc gaagcgaata ttgaggtggt cgatgaacat aatttaatgc
      181 ggattattag ccaaccgact aaactggtga agcgtgatta taaatatttt tctttgtttg
      241 ccttagaaga aggaacgatt atttctttac aaggttttaa atatccctta actaattatc
      301 atttagccat tcatgataat ttatgtttaa gcaacgaact ggttgaagat ggttttttgg
      361 aagttaataa caaaaaaata ttagtcattg aaagcaagta aaaccataaa aaaaaggctt
      421 taacagccct tttcggaatc attaagcgcg ttgcactaaa cctgacttca atgcttttgc
      481 ggaaacataa acgcgtttca ctttaccatc agtatcaacg attctgactt tttgtaaatt
      541 tgctttccaa ctacggcggg tagcaacaag tgagtgggga cgattatttc ctgtaactgt
      601 gcctaagccg gttatataac atttacgtgg catgatgaac ctccttaaaa atgtattgac
      661 tactttttac ttttaatatt ataccattaa ggccatttaa aggcaagtaa tatttacttt
      721 ttcgcctttt tattgttgat ttgtggggtt ttcatctgtt caaagcacat ttttcttgct
      781 agaaagcaag atttgtggta taacccttta atgtattagt atttgtaggt ggaatttaat
      841 aaaaatatat gaattttgat taattttcgg aggatttaat ggaaatcttt aaattagatg
      901 gattattatt caagacccta attgttaatg gggcagagaa tttaaaaata aattataaac
      961 aagtggatgc tttaaatgtt tttcccgtcc ctgatggtga taccggaacc aatatgcgga
     1021 tgaccttgga aggcggaatt aatgaaattt ataatctgga tgaatccaac atcggtgaat
     1081 tagccaaaag actgcaacgc ggaatgtta
//
LOCUS       DAOSGN010000385         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_140224_length_1108_cov_3.606838, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000385 DAOSGN010000000
VERSION     DAOSGN010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140224_length_1108_cov_3.606838"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PK087_05065"
     CDS             complement(<1..1001)
                     /locus_tag="PK087_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH18674.1"
                     /translation="MINKLNALLEDAIKDGVFPGVNYCLIADQVYFGSLGKRSLFPQP
                     EDNNLETIYDLASVSKVISTTTCIMKLLEQGKLRLYDQVVKYLPRFRHQDIVVWDLLT
                     HTSGLQADLPKASQLASREEALDKVFACDPIYPKNEKIVYSDIGFILLGMIVESISGL
                     SLDEFARLHVFEPLGMVDTCYNPLDKNRCAPTELRQDNIYQGYVRGHVHDEKAYILGG
                     VAGHAGVFSTVADLAHFMGMILNEGKYQGKVILSKPTIDLLFRKQVEVATGVSLDSDA
                     RGLGWIVRGNFCSAGDLVSDQTILHTGFTGTNIFIDRKNRIAFCMLSNRVHPTRDNV"
BASE COUNT          337 a          248 c          209 g          314 t
ORIGIN      
        1 ttcacattat cccgtgtcgg atggactcga ttagataaca tacaaaaggc aatccggttt
       61 ttgcgatcaa taaagatatt cgtaccggta aaaccggtat gtaaaatggt ttggtcgctg
      121 actaaatcac cggccgaaca aaagttaccg cggacaatcc accctaaacc ccgagcatcg
      181 ctatctaaag agactcccgt cgcaacttcc acttgcttac ggaaaagtaa gtcgatggtc
      241 ggttttgata aaattacttt cccttggtat tttccctcat ttaaaatcat ccccatgaaa
      301 tgagctaaat ccgcaacagt ggaaaaaacc ccggcatggc cggccacacc acctaaaata
      361 taagcttttt cgtcatgaac atgaccgcgg acataacctt gataaatatt gtcctgccgt
      421 aattccgttg gcgcacagcg gtttttatca agcggattat aacaagtatc gaccatgccc
      481 aagggttcaa aaacatgcaa acgggcaaat tcatctaatg ataaccccga gatgctttcc
      541 acaatcattc ccaacaaaat aaaaccaatg tcggagtaaa caattttctc gttttttgga
      601 taaatcgggt cacaagcaaa cactttatcc agcgcttctt cccgactggc taattgtgaa
      661 gctttaggaa gatcggcttg aaggccggaa gtgtgggtta acaaatccca aacgacgatg
      721 tcctgatggc gaaatcgtgg taagtatttc acaacttggt cgtaaagccg cagttttcct
      781 tgttccaata gtttcataat acaggtcgtt gtgctgataa ctttgctgac tgaagctaag
      841 tcgtaaatcg tttccaaatt attgtcttcc ggttgaggaa ataaacttcg ttttcctaaa
      901 ctgccgaaat aaacttggtc ggcaattaaa caataattga caccgggaaa aaccccgtcc
      961 ttaatagcat cttctaataa agcatttagt ttattaatca tgattttacc tcaaattgat
     1021 gaatatgacg atgatagcgt tgatagaaat tacgataaca gattttttcg atttctgttt
     1081 cttggtagcc acgctcccgc attttgct
//
LOCUS       DAOSGN010000386         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_140465_length_1107_cov_2.577947, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000386 DAOSGN010000000
VERSION     DAOSGN010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140465_length_1107_cov_2.577947"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PK087_05070"
     CDS             <1..862
                     /locus_tag="PK087_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOH18675.1"
                     /translation="CETKHNNLLSELGMKIKNDIGNIRLIAIAGPSSSGKTTFSHRLR
                     IELMTRGIKTVKISIDDYYLNREDAPLDEYGEPDFEHIGALDIDLFNQNLLSLIQGEE
                     VDTPVFNFEQGKRVGWHRVKLAKNTPIIIEGIHALNDQLTSLIPKNQKFKIYISPTSQ
                     INIDNHNPISPTEIRMLRRIVRDAKYRNTHPSVTFSMWASVRRGEFKWIYPFQEQADF
                     IFNTELTYELGVMKKYALPTLEKIDRDDEYFIPANRLIKFLKYFKDIDDRFVPCNSLL
                     REFIGESCFY"
     gene            840..>1107
                     /locus_tag="PK087_05075"
     CDS             840..>1107
                     /locus_tag="PK087_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOH18676.1"
                     /translation="MAKVVFIDMKESKDFQFTENLFKNLGQDYALLTAGVENDFNTMT
                     VGWATFGVIWGKNVITCYVRPSRFTYEFMEKNDYFTLSFYDARFI"
BASE COUNT          347 a          189 c          212 g          359 t
ORIGIN      
        1 gtgtgaaacc aaacataata atttattatc ggaattagga atgaaaatca aaaatgacat
       61 cgggaacatc cgcctgattg ccattgccgg accttcatca tccgggaaaa ccaccttttc
      121 ccatcgcttg cgcattgagt tgatgacgag aggcattaaa acagtaaaaa tttccattga
      181 tgattattat ttaaatcgtg aagatgctcc cttggatgaa tatggagagc ctgattttga
      241 acacatcgga gccttggata ttgacttgtt taatcaaaat ttattgtcat taatccaagg
      301 agaggaagta gatactcccg ttttcaattt tgaacaaggg aaacgagttg gctggcatcg
      361 ggttaaatta gcgaaaaaca cgccgattat cattgaagga atccatgctc tcaatgatca
      421 gttgactagt ttaattccca aaaatcaaaa gtttaaaatt tacatcagtc ctacttcaca
      481 aataaatatt gataatcata atccgattag tccgacggag attcgcatgt taagaagaat
      541 tgttcgcgat gcaaaatatc gtaatactca tccttccgtt actttttcca tgtgggcttc
      601 ggttcgaaga ggtgaattta aatggatata ccccttccaa gagcaagctg attttatctt
      661 taatacagag ttgacttatg agttaggggt tatgaaaaaa tatgcccttc cgaccttgga
      721 aaaaattgat cgtgatgatg aatactttat tcctgctaac cgtttaatta aattcctcaa
      781 atactttaaa gatattgatg atcgttttgt tccttgtaac tctttgttgc gagaatttat
      841 tggcgaaagt tgtttttatt gatatgaaag aaagcaaaga ttttcaattt acagaaaatt
      901 tatttaaaaa ccttggtcaa gattatgcct tattaacggc gggagtggaa aacgatttta
      961 acaccatgac cgtcggttgg gcgacttttg gtgtgatttg gggtaaaaat gtgattactt
     1021 gctatgttcg gccgtcccgc tttacctacg aatttatgga aaaaaatgac tattttaccc
     1081 tttcctttta tgatgctcgc tttatag
//
LOCUS       DAOSGN010000387         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_141481_length_1102_cov_3.097421, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000387 DAOSGN010000000
VERSION     DAOSGN010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141481_length_1102_cov_3.097421"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PK087_05080"
     CDS             <1..453
                     /locus_tag="PK087_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18677.1"
                     /translation="MAGFVLKEFSHNLFFGQQLLAQNYESMAILYYLLLSYLGQKDLY
                     GALALIKKSRLLQQKEYSAFHNPENANYAQLLNLPDADLYERLAILVMLYWESLGREF
                     SYDNCQDEALLLVRWFDLLNTLYELGYPKEMMDELQKVASIVFPFEEK"
     gene            468..>1102
                     /gene="tig"
                     /locus_tag="PK087_05085"
     CDS             468..>1102
                     /gene="tig"
                     /locus_tag="PK087_05085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOH18678.1"
                     /translation="MNVNIERSPEDKVLLTVELTKKEFAFYYEEELKKLMAKAELKGF
                     RKGKVPREIYLKRFGEAQVYQNALDSALTASYYQAVEDNKVQVIDEPKIDIDFELFDK
                     KKTLKYKAEVLVYPEVVLGKYFGVEVKKDSTEVEESEVEAAINRDLRSKSDLELVEEG
                     SLELGHTAVFDFEGRVDGVLFEGGTAKNYSLEIGSGQFIPGFEEQMVGMKQ"
BASE COUNT          352 a          171 c          252 g          327 t
ORIGIN      
        1 atggccggtt ttgttttaaa ggagttttcc cataacttgt tttttggtca acaattgttg
       61 gcccaaaatt atgaaagcat ggcgatactt tattatctgc ttttgtctta tttaggtcaa
      121 aaggaccttt atggtgcttt agctttgatt aaaaaaagcc gtttgttgca acaaaaagaa
      181 tattccgctt ttcacaatcc ggaaaatgcc aactacgctc aattgttaaa cttacccgat
      241 gctgatcttt atgagcgctt agccatttta gtaatgcttt actgggaaag tttagggcgg
      301 gagtttagtt acgataattg tcaagatgaa gctctcttgc ttgtgcggtg gtttgatttg
      361 ttgaatacgc tttatgagct gggatatcca aaagaaatga tggatgaact gcaaaaggtg
      421 gcatccatcg tctttccatt tgaagaaaaa tagaggagaa ataaataatg aatgttaata
      481 ttgaaagaag ccccgaggat aaagtcctct taacggttga attgactaaa aaagaattcg
      541 ccttttacta tgaagaagaa ttaaaaaaat taatggccaa agctgaactt aaaggttttc
      601 gaaaaggaaa agttccccgc gaaatttatt taaaacgctt tggtgaagcc caagtttacc
      661 aaaatgcttt ggacagtgcc ttaacagctt cttattatca agcggttgaa gacaacaaag
      721 tacaggtcat cgacgaaccg aaaatcgata tcgactttga attattcgat aaaaagaaaa
      781 ccttgaaata caaagctgaa gtcctcgttt atccggaagt ggtcttaggg aaatactttg
      841 gagtggaagt aaaaaaagac agtactgagg tcgaggaaag tgaagttgaa gccgccatca
      901 atcgcgattt aaggagtaaa tcggatttgg aactggtgga agaaggtagt ttagaacttg
      961 gtcatacggc ggttttcgat tttgaaggtc gtgttgacgg cgttttgttt gagggcggaa
     1021 cggccaaaaa ttattctttg gaaattggtt ccggccaatt cattcccggt tttgaagagc
     1081 aaatggtggg catgaaacaa ga
//
LOCUS       DAOSGN010000388         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_141674_length_1101_cov_3.173040, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000388 DAOSGN010000000
VERSION     DAOSGN010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141674_length_1101_cov_3.173040"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1101)
                     /gene="mutL"
                     /locus_tag="PK087_05090"
     CDS             complement(<1..>1101)
                     /gene="mutL"
                     /locus_tag="PK087_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOH18679.1"
                     /translation="KLCVLPHATSKIRNEKDLFKIATLGFRGEALPSIVAVSLFKLKT
                     SADGVRGLMYSLKAGEIVSEAMIAFPKGTEITVRNLFFNTPARLQNLQSASIELGYIL
                     DYVSKMALAHPNIAFSVINNDRQVLQTFGHNRLLEVITNIYKDEVAKSMIEFFTNNGY
                     FQISGLISKLNITRSSRHHITIIVNGRVVKNNKLVQAVIDAYEERLMGGRYPIAIINI
                     AVDLALVDVNIHPAKLEVRFSSEEELIALLKQEVSQTLAKTNLIVDLSDSKPRENDKI
                     DELSLDEALPPLTETSSQSQEQLLKEPIFIKQTLETDFVKEEAVPAYQLPPLEKIEKT
                     IESFSQQQYTLKDDQIRDDGELKQAHLPLLNYI"
BASE COUNT          340 a          212 c          203 g          346 t
ORIGIN      
        1 aatgtagttt aaaagcggca aatgggcttg ttttagttcg ccatcatccc taatttggtc
       61 atcttttaaa gtatattgtt gttgggaaaa actttcaatc gttttttcaa ttttttccaa
      121 aggaggaagc tggtaagccg gcacggcctc ttcttttaca aaatccgttt ccaatgtctg
      181 tttaataaaa ataggttctt ttaaaagctg ttcttgtgat tgtgaagatg tttcagtcaa
      241 gggaggcaaa gcctcatcta gcgataactc atctatttta tcattttccc taggtttaga
      301 atcgcttaaa tcaacaatta agttggtttt cgccagcgtt tgtgaaacct cttgcttcaa
      361 taaagcaatt agttcttctt cactggaaaa acgaacctct aatttagccg gatgaatatt
      421 gacatctacc aaggctaagt caacagcaat attgatgata gcgataggat aacgcccgcc
      481 cattaaccgt tcttcataag catcaatcac cgcttgcacg agtttattat ttttgactac
      541 ccgaccatta acaataatgg tgatatgatg acgggatgaa cgcgtgatgt ttaatttaga
      601 gattaagccg gaaatttgaa aatagccatt gtttgtgaaa aactcaatca tcgattttgc
      661 gacttcatct ttataaatgt tggtgataac ttctaaaaga cgattatgcc caaatgtttg
      721 cagtacttgt cggtcattat taatcacact aaaggcaata tttgggtgcg ccaaagccat
      781 tttggaaaca taatctaaaa tatatcccag ttctattgaa gcggattgta agttttgcaa
      841 acgagcgggc gtattaaaaa ataagttgcg aacggtaatt tccgttcctt tcggaaaagc
      901 aatcatcgct tccgaaacga tctcaccagc ttttaaagaa tacatcaaac cacgaacacc
      961 atcagcgctg gttttcagtt taaacaagga aaccgccaca attgaaggta gagcttcccc
     1021 ccgaaatccc aaggtcgcaa ttttaaacaa atctttttca ttgcgaattt tactggtagc
     1081 atggggaaga acgcataatt t
//
LOCUS       DAOSGN010000389         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_141919_length_1100_cov_2.433493, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000389 DAOSGN010000000
VERSION     DAOSGN010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141919_length_1100_cov_2.433493"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1100)
                     /locus_tag="PK087_05095"
     CDS             complement(<1..>1100)
                     /locus_tag="PK087_05095"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOH18680.1"
                     /translation="IKVKSYDAEITLDNNGDMFISETWVVDWPTGKHVSFRDIAYQKY
                     NPDNPLYQEETNVALFDNDSVNVAVFGKDGLALPKSQYKVGFSFKGDVDELGQPVECY
                     PSRSNCESIFIYVDDGMQSTMTFKYDYKIIGAVTKYQDTNELNWVLLEYFESGVDDAM
                     VTVNIPGVAKKDLQAWGHGLSKGKVIISDEKVVLDIDRIKPSEALEFRILINDDVFNV
                     KPINFIDRAQYDEIVDYEVEWAKKTNLSIFLSFVVWVLAAAILIATVFVGVRVYIKYD
                     KEYEPQFQGKYFRELPAEYSPAEMSYLYYFKKIQNEDVTATLLDLVRRKYLILDNNTS
                     DINDKNPAFKITKNLAMNEDDLLPHEKHLISW"
BASE COUNT          344 a          233 c          183 g          340 t
ORIGIN      
        1 aaccaggaaa ttaaatgctt ttcatgagga agtaaatcat cctcattcat cgctagattt
       61 ttcgttattt taaaggcagg gtttttatca ttgatatcgg aagtgttgtt gtcaaggatt
      121 aagtatttgc ggcgcaccaa atctaaaagc gtggccgtaa catcttcgtt ttgaattttt
      181 ttgaaataat aaagataact catttccgcc ggactgtatt ccgccggtaa ttcccggaag
      241 tatttccctt ggaattgcgg ttcgtattcc ttatcatatt taatataaac ccgaacacca
      301 acaaagacgg ttgctattaa aatggcagct gctaaaaccc aaacgacgaa agacaaaaaa
      361 atacttaaat tagttttctt agcccattca acttcataat cgacgatttc atcatactgt
      421 gctctatcaa taaagttaat aggcttgaca ttgaaaacat catcattgat gagaatccta
      481 aattctaagg cttcacttgg tttaatgcga tcaatatcca gaacaacttt ttcatcactg
      541 atgataacct tcccttttga taatccatga ccccaagctt gtaaatcctt ttttgctaca
      601 ccggggatat tgacggtcac catggcatca tcgacacccg attcaaaata ttccaataaa
      661 acccagttca actcattggt atcttgatat ttggttaccg caccgatgat tttataatca
      721 tacttgaaag tcatcgtgct ttgcataccg tcatcaacat agataaagat tgattcacaa
      781 ttagaacgcg agggataaca ttcgaccggt tgccctaatt catcaacatc acctttaaaa
      841 gaaaatccga ctttatattg gcttttcgga agggcaagtc cgtcctttcc aaacacggcg
      901 acattaacgg aatcattatc aaataaagcg acattggttt cttcttggta aagaggattg
      961 tccggattgt atttttgata agcaatatcc cggaaggaaa catgcttacc cgtgggccaa
     1021 tcaacaaccc aagtttccga aataaacata tcaccgttat tgtccagcgt gatttccgca
     1081 tcataactct tgactttgat
//
LOCUS       DAOSGN010000390         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_142713_length_1096_cov_2.375600, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000390 DAOSGN010000000
VERSION     DAOSGN010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142713_length_1096_cov_2.375600"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..570)
                     /locus_tag="PK087_05100"
     CDS             complement(151..570)
                     /locus_tag="PK087_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18681.1"
                     /translation="MDNQKQPNFWLRGKRETKKFYEIRLNRSTSIATFIFGFLLAAVI
                     VLPIGILIYQFLVIFGYNLAVFGLYLTLIWLALMFFNGLSNYLTVKIAQASVKDMLNL
                     QAIEAGYIFWYQLLNIGFGLFSLIIIIISAIQILGAR"
     gene            complement(563..>1096)
                     /locus_tag="PK087_05105"
     CDS             complement(563..>1096)
                     /locus_tag="PK087_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18682.1"
                     /translation="DLNQFMTLLNAQNNSDFFSLSVIRNNKEVVIQSRMIALVDEQQN
                     TIYRLGVEALPDYKIIDKFPKYKLNDKIDSIGGSGGAMLALAIYNSLLAEDITMGKTI
                     VGTGTINIDGKIGDIGAVSQKIVTASLFNVDYFFVNPEDYEEAYQKYQTINPSFELVK
                     VNTFQDILIYLEGEKHG"
BASE COUNT          387 a          197 c          160 g          352 t
ORIGIN      
        1 tttctaacaa aacaaattta aatattccgg gaatagcttt gcgattttgc aaatctgcaa
       61 aatttttaaa agcaattagt tttgtgattt gatcaataat gattcccata acaattaatg
      121 ataaaccaat taacatttgt tttcctttca tcatcgtgct cctaaaattt ggatggcact
      181 aataataatt attattaggc tgaacaaacc aaagccaatg ttcaataatt gataccaaaa
      241 aatataacct gcttcaatgg cttgtaagtt taacatatct ttaacactgg cttgagcaat
      301 tttaacagtt aaataattgg atagtccatt aaaaaacatt aaagctagcc aaataagcgt
      361 taaatataaa ccaaaaaccg ctaaattata gccaaagata actaaaaatt gataaattaa
      421 aatgccaatt ggtagaacaa tcaccgccgc taaaagaaag ccaaagataa aagtggctat
      481 tgaagtggaa cgattcaaac ggatttcata gaattttttg gtttctcgct ttccgcgtag
      541 ccaaaagtta ggttgttttt gattatccat gcttttcacc ctcgagatat attaaaatgt
      601 cttgaaacgt attaactttt acaagttcaa aagacggatt aatggtttga tatttttggt
      661 aagcttcctc ataatcttct gggttaacaa aaaaataatc cacattaaat aaactcgccg
      721 tcacaatttt ttgggacact gcgccgatgt cacctatttt cccatcgatg ttaatcgtgc
      781 ccgtccccac aatagtcttg cccatagtga tatcttccgc taataacgaa ttataaatag
      841 ccaaagcaag catcgctccc cctgagccac caatagaatc aattttatcg tttaatttgt
      901 atttagggaa tttatcaata attttataat ctgggagggc ttcaacgccc aaacggtaaa
      961 tggtattttg ctgttcatcc acaagggcaa tcatccgtga ttgaataaca acttctttgt
     1021 tatttcttat aacgcttaaa ctaaaaaaat cggaattatt ttgggcatta agtaaagtca
     1081 taaactgatt caaatc
//
LOCUS       DAOSGN010000391         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_142863_length_1095_cov_3.112500, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000391 DAOSGN010000000
VERSION     DAOSGN010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142863_length_1095_cov_3.112500"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..540
                     /locus_tag="PK087_05110"
     CDS             235..540
                     /locus_tag="PK087_05110"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH18683.1"
                     /translation="MDNKQYALVFKALGDENRIQMVLMLSQKELCARQFYEKLGISQA
                     MVSHHLRILREAKIIKGRKAGKGIYYSLNKDMIAEMLLFFDFLDFGNMQCACQEGFS"
     gene            678..>1095
                     /locus_tag="PK087_05115"
     CDS             678..>1095
                     /locus_tag="PK087_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOH18684.1"
                     /translation="MFNSPYLKRVYQDLLTKSGHEPEFLQAVNEFLASMDLLVDSDPE
                     IERLGIIERLVEPERMIIFRVPWLDDQGKIHVNRGFRVQYNSAIGPYKGGIRFDRSVN
                     QSIMKFLAFEQTFKNALTGLPMGGAKGGADFDPVGKS"
BASE COUNT          320 a          165 c          237 g          373 t
ORIGIN      
        1 gaccaccctc agtaatttga aattgaccac ctagcggttt aaccactccc ctttttcatt
       61 ttctaataat aagtgataga ttactcggca aggttactcg gtaaaaaatt gcattttaat
      121 atttttgaat tgttttaatt tatcttgcta tcatacagtg agttattctc tttgtagatt
      181 caaattaaat gcaacaatat caatatataa ttaatcatca tagttggagg aattatggat
      241 aataagcaat atgcattagt gtttaaagct ttgggggatg aaaatcgcat ccaaatggtc
      301 ttgatgttat cccaaaaaga gctttgtgct cgtcaatttt atgagaaatt ggggatttcc
      361 caagcgatgg tctcgcatca tttgcgaatt ttgagggaag cgaaaattat caaaggtcgt
      421 aaagcgggta aagggattta ttattcgctc aataaggaca tgattgcgga aatgttgttg
      481 ttttttgatt tccttgattt tgggaatatg caatgtgctt gccaagaagg cttttcatga
      541 tgatttagta agattaaaac attaacttaa gtcaactaaa gcgggaatga tttctcgctt
      601 tttttccttt gccattttca tattgtcaac gatttttatt tgtggtagaa taaaagagtg
      661 aagaaaggaa ttattcgatg tttaatagtc cttatttaaa gagagtatat caagatttat
      721 taactaaaag cggccatgaa ccggaattct tgcaagctgt taatgagttt ttggcttcga
      781 tggatttatt ggtggatagt gatcccgaaa ttgaacgttt gggtattatt gagcgtcttg
      841 ttgaaccgga gcgcatgata attttccgag tgccttggct tgatgatcaa gggaaaatcc
      901 atgttaaccg agggtttcgg gtgcaatata attctgccat tggtccttat aagggcggaa
      961 tccgcttcga tcgctccgtt aaccaatcga ttatgaagtt tttagccttt gaacaaactt
     1021 ttaaaaacgc tttaaccggg cttccgatgg ggggcgctaa gggcggagct gattttgatc
     1081 cggtgggaaa aagtg
//
LOCUS       DAOSGN010000392         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_142977_length_1095_cov_1.783654, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000392 DAOSGN010000000
VERSION     DAOSGN010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142977_length_1095_cov_1.783654"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1095)
                     /locus_tag="PK087_05120"
     CDS             complement(<1..>1095)
                     /locus_tag="PK087_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH18685.1"
                     /translation="IVWNSAQPELISAMGKVTQPEETTKVSMRAEITGLPFAVSFQYD
                     LQINGLNTVEPVEIYFIDLGKYGQSDSGESIYFRIGAYDVLVDAGDNYKTSNQAIKEV
                     IDKYSEDRIIDLVIATHPDADHIGGMSFVFSEYQVLNLLQFHGTHTTQLYEKYVSSYQ
                     AEGLVKECLITDAMNNANGCSQIIEIAPSVTINLIETGYYETVEPNGRSIVFILEAYG
                     TKVLLTGDADNNDGRTVEANYQQAVGDIDILKAVHHGTSHGSTLEFLKSSRPEVVIIT
                     NGNYFGNKHGHPTADAINRIYQANNQTQIYAVVGGDVLDCTVTASNSYQCNVTDRFLD
                     RNGTINITIDNHGYEIQVEYFDMPLELSKTQ"
BASE COUNT          312 a          236 c          212 g          335 t
ORIGIN      
        1 ctgtgtcttt gataattcca aaggcatatc gaaatattcg acttgaatct cgtaaccgtg
       61 attatcaatc gtaatattaa tcgtcccatt gcgatctaaa aagcgatccg ttacattgca
      121 ttggtaacta ttggaggctg tcaccgtaca atccagaaca tcacccccaa caaccgcata
      181 aatttgtgtt tgattgttcg cttgataaat gcggttgatg gcatcggcgg tcggatggcc
      241 atgtttgttt ccaaaataat tcccgttggt aatgatgacg acttccggtc ttgatgattt
      301 taaaaattcc aaagtcgaac catggctggt gccatgatga accgctttaa gaatatcaat
      361 atcaccaaca gcttgttgat aattagcttc aaccgtacgg ccgtcgttat tatcagcatc
      421 accggttaat aaaacttttg tgccgtaggc ttccaaaata aagacaatac tgcgaccgtt
      481 cggttcgacc gtttcgtaat aaccggtttc aattaagtta atggtaaccg agggcgctat
      541 ttcaatgatc tgtgaacagc cattggcgtt attcatggca tcggtaataa ggcattcttt
      601 aactaaaccc tccgcttgat aactgctgac atacttttca tataattgcg tggtatgtgt
      661 tccgtggaat tggagcaagt ttagcacttg gtattcacta aaaacaaaac tcataccacc
      721 aatatgatcg gcatcgggat gggtagcaat cactaaatca ataattcgat cctcactata
      781 cttatcaatg acttctttaa tcgcttgatt cgatgtttta tagttatcac cggcatcaac
      841 taagacatca taagcaccga tgcgaaagta aattgattct ccggaatcgc tttgcccgta
      901 ttttcctaaa tcgataaaat aaatttctac tggttccacg gtatttaaac cgttgatttg
      961 cagatcatat tgaaaactaa ccgcaaaggg taagccggta atctcggccc tcatcgaaac
     1021 tttggtcgtt tcttctggtt gggtgacttt ccccatagcg gaaataagct cgggttgcgc
     1081 actgttccaa acgat
//
LOCUS       DAOSGN010000393         1086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_144822_length_1086_cov_2.167798, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000393 DAOSGN010000000
VERSION     DAOSGN010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144822_length_1086_cov_2.167798"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..>1086)
                     /gene="dapF"
                     /locus_tag="PK087_05125"
     CDS             complement(271..>1086)
                     /gene="dapF"
                     /locus_tag="PK087_05125"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOH18686.1"
                     /translation="TMKFAKYHGLGNDFIIIEDGPYDYSQLAVDLCDRHIGVGADGLI
                     IVNREPLVMGFYNMDGSEGTMCGNGLRCFAQYCMDNGIVTADEFIVRTKAGLRKMQYL
                     GNNLWQTSLGKPEFDPKLMDIATKAPQFINEPMLGVRVSAVFTGTTHAVVFVLNINSA
                     EVSDLGERISNHSLFLKQTNVNFVQVLDRMNFSVRTYERGVGWTLACGTGAAAGFVMG
                     HLKDYCDDAVNIHFALGHLHVQQTENGELLLTGPAEKICTGDFLKTKRLKRLI"
BASE COUNT          360 a          238 c          164 g          324 t
ORIGIN      
        1 cgcaacattt tctttaattt ccatttttat acctcttttt ttgttggggt atccgcccaa
       61 ttatcttatt cttattatac ctttttaatt ggtaaaaagc aattattttt cttattttga
      121 aaaaaggaca aaaaatacca ccaaaaaaaa cacattacga tgaaaaaaac aagtgttttt
      181 tatctttata tcaaacgaaa taaaaaaatg acaccaccaa agtgctttga aactaacttt
      241 aagttttttc aagcatttac ttgggaagta tcatatcaat ctttttaagc gttttgtttt
      301 taaaaaatcg cccgtgcaaa ttttttcggc cggaccggtt aacaatagtt caccgttttc
      361 cgtttgctgc acatgcaaat gcccgagggc aaaatgaata ttgacggcat catcacaata
      421 atcttttaaa tgccccatca caaaaccggc agcagccccg gttccacaag ccaaagtcca
      481 gcctacaccc cgttcataag tccgaacaga aaaattcatt cgatccaaaa cttgtacaaa
      541 attaacattg gtttgtttta aaaagaggga atggttgctg attctttcac ctaaatcgct
      601 aacttcagca gagttaatat ttaagacaaa gacgaccgca tgcgtggttc cagtaaaaac
      661 agcactaaca cgaaccccga gcatgggttc attaataaat tggggcgcct tggtggcaat
      721 atccattagt ttaggatcaa attccggttt gcctaagcta gtttgccata aattgtttcc
      781 taaatattgc atttttctta aaccagcttt agttcgaaca ataaactcat cagcagtgac
      841 aatcccgttg tccatgcaat attgggcgaa gcaacgcaaa ccattaccgc acattgttcc
      901 ttccgaaccg tccatattat aaaaacccat cacgagtggt tcacgattaa caatgattaa
      961 gccatccgca ccaacaccaa tatggcgatc acataaatca actgccaatt gcgaataatc
     1021 gtaaggacca tcctcaatga taataaagtc atttcctaaa ccatggtact tcgcaaattt
     1081 catagt
//
LOCUS       DAOSGN010000394         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_145010_length_1085_cov_2.226214, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000394 DAOSGN010000000
VERSION     DAOSGN010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145010_length_1085_cov_2.226214"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PK087_05130"
     CDS             <1..612
                     /locus_tag="PK087_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH18687.1"
                     /translation="PAVASVDETGRVVALSAGTTTITAHSTIAPVVSTAEITVLPVEQ
                     RSVVTTYSYTPISFTGARKMYVSDLVTLATTGYNLGDILWTSSNNAVAAVSMYDGRVE
                     ALAKGNTVIKATLIANPQIYTTVSIQVAEVTPSITFSENSLDRLNSLKTVYINAINPN
                     SVTWKGTLKLRKDAKIYVPAQNYDAYLKVWKDYADMIYIKENE"
     gene            695..>1085
                     /locus_tag="PK087_05135"
     CDS             695..>1085
                     /locus_tag="PK087_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18688.1"
                     /translation="MTKTDFNQLYNNFTNIAMEARISFRIKKLLFELPSSEDGFNKLI
                     TTIQETNITKAQALNDIEDFFLTMKYCYSGYEYFSRRIDFDTLKRNIIDLLEKSSNAY
                     ISSIALGEMIFNQLSGHLNDGHFSIIGF"
BASE COUNT          338 a          203 c          215 g          329 t
ORIGIN      
        1 cccgctgtcg cttcggttga tgaaacggga agagtagttg ctttgagtgc gggaacaaca
       61 acgattaccg cccattccac aatcgctccg gtagtttcca ccgctgaaat taccgtgttg
      121 ccggtcgaac aacgaagtgt cgttacgact tattcatata caccgatcag ttttacgggc
      181 gcccgcaaaa tgtatgtttc cgatttagtg acattagcaa ccaccggcta taatcttggt
      241 gatattctat ggacgagttc caacaatgcc gtggccgcag tatcaatgta tgatggacga
      301 gttgaagcct tagcgaaggg caatactgtt atcaaagcga ctttaattgc caacccgcaa
      361 atttatacaa cggtcagcat tcaggttgcg gaagttactc cgtcaattac tttttcggag
      421 aattctttag accgtttaaa cagtttaaaa acggtttata tcaatgccat taatcccaat
      481 agcgtcactt ggaagggcac tttaaaactg cgcaaagatg cgaagatata tgttccggcc
      541 caaaactatg atgcttacct aaaggtttgg aaagattatg cggacatgat ttatatcaag
      601 gaaaacgaat aaacacccca acgggtgttt ttccatttcc ccaagaagat gattttgtca
      661 tcattgatga ttgatttaac gggatggaga taagatgact aagacggatt ttaatcagtt
      721 gtataataac tttaccaata ttgccatgga agcaaggatt agttttcgca ttaagaaact
      781 actttttgaa ttgccttctt cggaagacgg ttttaataaa ttaataacaa caatccaaga
      841 aactaatata accaaggccc aagccctaaa tgatatcgaa gactttttct taaccatgaa
      901 gtattgttat agtggttacg agtattttag tagaagaatc gattttgata ctttaaaaag
      961 gaatatcatt gatttattag aaaagagttc aaatgcttat atttcttcaa ttgctttggg
     1021 agaaatgatt tttaatcaac tttctggcca tcttaacgat ggacactttt caatcatagg
     1081 ttttg
//
LOCUS       DAOSGN010000395         1083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_145433_length_1083_cov_2.137160, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000395 DAOSGN010000000
VERSION     DAOSGN010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145433_length_1083_cov_2.137160"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>1083
                     /locus_tag="PK087_05140"
     CDS             71..>1083
                     /locus_tag="PK087_05140"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH18689.1"
                     /translation="MKATLKNTTFTAEVTFTVTPTPVELFDMFHEENPIIKNITVFGW
                     ETNGTYNYDMYSSVSKYLAEPLVITEAMISPSLDNRPGIIKQSTEYITVHDTASAAAS
                     AGAVAHSSYVTNGGGGTSWHYTVGTDGIFHHIPNNEVAYHARDGSRLYAMSQTNNDSG
                     IVASGKNKPLLTITTDGYYALNGQKTRILAPTNNGAILTTAHINDYGIHTIVGPNGNW
                     WIGNSWYSSTYKLIGNAGGNRNSVGIESCVNKGSDVFLTWQLLAKLVAFLLEEQNLGL
                     DRVVQHHYYSGKDCPMTMRHAGMWDYFLEMVYAEYLVRTVLKDYTITFQSSNPELISA
                     NGR"
BASE COUNT          318 a          262 c          220 g          283 t
ORIGIN      
        1 gagcaatgac gccgtggcca ccgtggatgc ggacggcaaa gttaaaccag taggaccagg
       61 aaaggtaact atgaaagcta ctttaaaaaa tacgactttt accgccgaag tgacatttac
      121 tgtaactccg acaccagttg aattattcga tatgttccat gaagaaaacc ccatcatcaa
      181 aaacatcacc gttttcggtt gggaaactaa tggcacttat aactacgaca tgtattctag
      241 tgtcagcaaa tatttagctg agccgctcgt tatcactgaa gcgatgattt ctccctcact
      301 tgataaccga ccgggcataa tcaaacaaag cacggaatat attactgtcc acgataccgc
      361 ttcagccgct gcctcagccg gtgctgtcgc ccatagttcc tatgtcacaa atggtggtgg
      421 tggaacctct tggcattata ccgttggtac ggatggtatc ttccatcata tccccaataa
      481 cgaagttgct taccatgccc gtgatggctc ccgcctttat gctatgagcc aaaccaacaa
      541 cgatagcggc attgttgctt ccggaaaaaa caaacccctt ctcaccatta ctacagatgg
      601 ctattatgct ttaaacggtc aaaaaactcg catcttagct ccaaccaaca acggtgcaat
      661 tctcacaact gctcacatta acgattacgg cattcacacc attgtcggtc ctaacggcaa
      721 ctggtggatt ggtaattctt ggtacagttc cacttataaa ttaattggca atgccggtgg
      781 caaccgcaat agcgtcggca ttgaatcctg tgtaaacaag ggaagcgatg ttttcctaac
      841 ttggcaactt ttagcaaaac tagtcgcctt cttattggaa gaacaaaact tgggtcttga
      901 ccgcgtcgtt caacatcatt actacagcgg caaagattgc ccgatgacaa tgagacatgc
      961 cggaatgtgg gattatttcc tagaaatggt ttatgcggaa tacttagtga gaactgtttt
     1021 gaaagattac acaattactt tccaatccag caatccggaa ttaatcagtg ccaatggccg
     1081 cat
//
LOCUS       DAOSGN010000396         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_146931_length_1076_cov_2.175318, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000396 DAOSGN010000000
VERSION     DAOSGN010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146931_length_1076_cov_2.175318"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1076
                     /locus_tag="PK087_05145"
     CDS             <1..>1076
                     /locus_tag="PK087_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18690.1"
                     /translation="KANSTLFRSRDYDLLMSLPPKPSTIIASKLVYILAINYLLFAFI
                     YFPTIIVYTVFNTTDLWFWLLVLPTFFLVPLLPIAVSSLLAYLFGFITPKIKYKNLTS
                     ILFSLLILFLIMYASFQSSSIEEDPSAFALFMKNALVKIYYPGQIAFLGMLGSWQDYL
                     LFTLISVVPFVGFVWLLSKNYMGANSRGNSSYINKNYEMKMMQSSDQKKAMIKKELKR
                     YFGSSVYVLNTIMGPMMSTFLILFIFFGEDSLSATIPAGTITPDIISMIIVGIAIFTM
                     GMTSTTASSISIEGKQFWILKSLPLSPQQIFNGKLFVNYLISFPFVIINGIIALVAFR
                     FSLWSALFMFLIPGLVVVIMSYAG"
BASE COUNT          266 a          207 c          190 g          413 t
ORIGIN      
        1 cgaaagccaa cagtactttg tttagaagcc gtgattatga tttgttaatg agtttaccgc
       61 ctaaaccatc aacaattatt gctagcaaat tggtttatat tttagcaatc aattatttac
      121 tgtttgcctt tatttatttt cctacgataa tcgtttatac ggtttttaat accactgatt
      181 tatggttttg gcttttagta ttgccgacct ttttcttggt tccgctcctc cccattgcgg
      241 tctcttccct gctggcttat ttatttggct ttattactcc gaagattaaa tataaaaatt
      301 taacttccat tttgttttcg ttgttaatct tgttcttaat catgtatgcc agttttcaat
      361 cctcgagcat cgaagaagac ccaagtgcct ttgccttgtt tatgaaaaat gctttggtaa
      421 aaatttatta tcccggtcag attgcttttt taggtatgct ggggagttgg caagattatc
      481 ttcttttcac attaatttcc gttgtaccgt ttgtaggatt tgtgtggttg ttaagtaaaa
      541 actacatggg ggctaattct cgtggcaaca gtagttatat caacaaaaac tatgaaatga
      601 aaatgatgca atcttccgat caaaagaaag ccatgattaa gaaggaatta aagcgttatt
      661 tcggctcttc cgtctatgtt cttaatacta taatgggacc gatgatgtca acctttttaa
      721 ttttgtttat cttttttggt gaggattccc tttccgcaac cattcccgcg gggacaatta
      781 cccccgacat catttccatg attattgtgg ggattgcgat tttcaccatg ggcatgactt
      841 ccacaaccgc tagttcgatt tccattgaag gtaagcagtt ttggatttta aagagtctac
      901 cgctttcacc ccaacaaatc tttaacggta aattgtttgt caactattta atctcgtttc
      961 cctttgtcat cattaatggc atcattgcct tagtagcttt tcgcttttcc ctttggagtg
     1021 ctttatttat gttcctcatt cccggtttag tagtcgttat aatgagttat gccggc
//
LOCUS       DAOSGN010000397         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_147152_length_1075_cov_2.114706, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000397 DAOSGN010000000
VERSION     DAOSGN010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147152_length_1075_cov_2.114706"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..>1075
                     /locus_tag="PK087_05150"
     CDS             67..>1075
                     /locus_tag="PK087_05150"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOH18691.1"
                     /translation="MADKTQDTVKEVIEDIYQPPVTLASEFATYEEVSVNVNPKVPAY
                     IVNQDLSNVTNVGYFYFSDAAKEMLVKNAFVVVPSYHDEFFTLYETNRYSYIPSLITT
                     DSILHNYHLMFDFLLRQLEEQKLAVELKQLNANLLVQSLSQYNNLQGTEWENAAKRNV
                     GFFAVGSKLLDSSVDVPSIVANEVNQELALIEAHQGIEKSPVMNIGGGLDVMIDTPQG
                     PQALKALKEDYSQYIPRGHYDKTEELKAYFKSMMWYGRLTFRMKNEDEIKSALLITLA
                     LNQGDNQNSWNKIYEPVNFFVGKSDDITYYQFKDLAEKIYGPNVAIESFLDDKDKFAS
                     FV"
BASE COUNT          330 a          176 c          234 g          335 t
ORIGIN      
        1 ttaggagcaa ttattatcat tgtcgccggg ggagcttatt ttattttacc gaaattaatt
       61 caaaaaatgg ctgataaaac tcaggatact gtaaaggaag ttattgaaga tatttatcag
      121 ccgcccgtta cccttgcttc ggaattcgcg acttatgaag aggtgtcggt aaatgttaat
      181 cccaaagttc ccgcttatat tgtgaatcaa gatttgagca atgtcactaa tgtgggctat
      241 ttttattttt ctgacgcggc aaaagaaatg ctagttaaaa atgcttttgt ggtcgtgcct
      301 tcttatcatg atgaattttt tactctttat gaaacaaatc ggtattcgta tataccgagc
      361 cttattacga ctgactcgat actccataat tatcatttga tgtttgattt tttattgaga
      421 cagctggaag aacaaaaatt ggcggtcgag ttaaagcagc ttaatgctaa tttattggtc
      481 caatcgttaa gccagtataa taatttgcaa ggcactgaat gggaaaatgc cgccaagaga
      541 aatgttggat tttttgccgt gggcagcaag cttttagatt cttcggttga tgttccgtcg
      601 atagtggcca atgaagtcaa tcaggagctg gctttaatcg aagcccatca gggcatagaa
      661 aaatcaccgg tgatgaatat tggcggcgga ttggatgtaa tgatagacac cccgcaagga
      721 ccgcaagcct taaaagcgtt gaaagaagat tattcgcagt atattcctcg ggggcattat
      781 gacaaaaccg aagagttgaa agcgtatttc aaatctatga tgtggtatgg ccgtctcact
      841 ttccggatga aaaatgagga tgaaattaaa tcggcattgt tgataacttt agctcttaat
      901 cagggggaca atcaaaattc ttggaataaa atttatgagc cggtgaattt ttttgtcggc
      961 aaaagtgacg acatcactta ttatcagttc aaggatttgg ccgaaaaaat ttatggtcca
     1021 aatgtagcaa tagaatcatt tttagacgat aaagataaat ttgcctcttt cgtta
//
LOCUS       DAOSGN010000398         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_147191_length_1075_cov_1.771569, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000398 DAOSGN010000000
VERSION     DAOSGN010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147191_length_1075_cov_1.771569"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1075
                     /locus_tag="PK087_05155"
     CDS             <1..>1075
                     /locus_tag="PK087_05155"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOH18692.1"
                     /translation="ICTLIEKRLEIAKQIGLFKLKNQLPIVSLEREQEINKRLQKRKY
                     GEQLSSIYEKIFQLSKELQQIDFFLVGKKLDYSLSPLIHLLMGNLRYGLWETEHFESI
                     QKLPFQALNVTNPFKTAAFECCDESSEEAKITKTCNLLVRNNGIIRGFNTDYGAFLAT
                     LDFYHQNVKSLKVVIIGNGATSRTIAQALIARGCDRIIYLVREIRNKDEFLIADYLRF
                     SDFQMIINTTPYGTNQQDDLLPLFPLKDFVNLQFVMDVVYNPPLTALLLAAKQLSIPH
                     ANGLLMLVTQAALGLQIVKQKSYHNLIQPVFQQLKKELTNLVLIGMPYSGKTTYGRLI
                     SKALGRPFFDVDLELEKEGHDLST"
BASE COUNT          355 a          182 c          192 g          346 t
ORIGIN      
        1 atatgtacct taattgaaaa acgcttggaa atagccaagc aaattggcct tttcaaatta
       61 aagaaccaat taccgattgt ttctttagaa cgtgaacaag aaataaataa acgtcttcag
      121 aaaagaaaat atggcgaaca actaagcagt atttatgaaa aaatttttca attaagtaag
      181 gaattacaac aaattgattt ttttcttgtt ggtaagaaat tagattattc cttatcaccg
      241 ttaattcatt tattaatggg aaatctgcga tatggtcttt gggaaacgga acattttgaa
      301 tccatccaaa aacttccttt ccaagctcta aatgttacta atccttttaa aactgccgct
      361 tttgaatgct gtgatgaaag cagtgaagaa gccaaaataa ctaaaacatg taatttatta
      421 gtccgaaata acggcatcat tcgtggcttt aatacggatt atggggcctt tttagcaacg
      481 ctcgattttt atcatcaaaa tgttaaatca ttgaaggtcg tcattatcgg gaatggtgcc
      541 acaagccgaa cgattgccca agctttaatt gctcggggtt gcgatagaat aatctattta
      601 gtgcgcgaaa taagaaataa ggatgagttt ttaattgctg attatttgcg ttttagtgat
      661 tttcaaatga ttataaatac aaccccttac ggaacgaatc agcaagatga tttgttgccg
      721 ctctttcccc taaaggattt tgttaatttg caatttgtga tggatgtcgt ttataatccc
      781 cccttgacag cattattgct ggcggctaaa caactttcta ttccccatgc caatggtcta
      841 ctaatgttag ttactcaggc cgctttagga ttgcaaatcg ttaagcaaaa gagttatcat
      901 aatttaatac aaccggtttt ccaacagtta aaaaaagaac taaccaatct tgttttaatt
      961 ggaatgcctt attccggaaa gactacttat gggcgactaa tcagtaaagc actgggaaga
     1021 cctttttttg atgttgattt agaacttgag aaagagggac atgacctttc aacct
//
LOCUS       DAOSGN010000399         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_147259_length_1074_cov_3.371933, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000399 DAOSGN010000000
VERSION     DAOSGN010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147259_length_1074_cov_3.371933"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PK087_05160"
     CDS             complement(<1..916)
                     /locus_tag="PK087_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOH18693.1"
                     /translation="MRYRIEHDALGAKQIPAEAYYGIGSYRSKEAFNITKHGLLRQMI
                     KAFASLKKAFAKTNLDLGYLDKKVANVISLSCDEILNGRLHGQFVTDIVQGGLGLAMD
                     KNANEVIANRANEMLGGEKGNYEFVSLQAVALNQLPKDNVIIAGRISAIRLSKKLLME
                     AKKLSNALEDRKHNPSFSPIKKEWEAFNVILEKDMKRVDEALNHLKFIPVFVDLSKTE
                     ESNLAYAKKFVKNISQACGEDLELAKNMVENRRNLDGFAWLSSALKQMMMDLSKIAKD
                     LELMHKQKQLKLPQIEVDDYFSALEVIKQ"
BASE COUNT          314 a          229 c          177 g          354 t
ORIGIN      
        1 tttgcttaat tacttctaaa gcgctaaaat aatcatcaac ttctatttga ggaagtttta
       61 attgcttttg tttgtgcatc aattctaaat ctttagcaat cttggataaa tccatcatca
      121 tttgtttgag cgctgatgac agccaagcaa agccgtctaa attgcgacgg ttttcaacca
      181 tattcttggc taattccaaa tcctcaccgc aagcttggga aatgtttttc acaaactttt
      241 tcgcataagc caaattagat tcttccgttt ttgataaatc cacaaacacc ggaataaatt
      301 ttaaatggtt gagagcttca tcaacccgtt tcatgtcctt ttccaaaatg acattgaaag
      361 cttcccattc ttttttaatt ggtgaaaaac taggattatg tttgcggtct tccaaagcat
      421 tggataattt tttcgcttcc atcagcagtt ttttgcttaa gcggatggcg gaaatccggc
      481 cggcaataat gacattatct ttaggaagtt ggtttaaagc tactgcttgt aacgacacaa
      541 attcatagtt gcctttttcc ccacccaaca tttcattggc acggttggca atcacttcat
      601 tggcattttt atccatggct aaacccaaac cgccctgaac aatatcggtt acaaactgac
      661 catgtaaacg gccgtttaaa atttcatcac aacttaaact aatgacattg gccactttct
      721 tatctaaata ccctaaatcc aagttggttt tagcgaaagc tttttttaag gaggcaaaag
      781 ctttaatcat ttgtcttaac aagccatgtt tagtaatatt aaacgcttct ttcgaacgat
      841 aactgccgat tccgtaataa gcttcggcgg gaatttgttt ggcacctagg gcatcatgtt
      901 caatccgata ccgcattatt cgtccctcgt attgataacg gcttcacaaa cggcatcaac
      961 aaccttgtta ttaaacactg acggaataat atattccggg cttaattcgt ctttagaaat
     1021 taaagaagca atagcatagg ctgccgccac acaaattttt tgggtaatct tccg
//
LOCUS       DAOSGN010000400         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_148196_length_1070_cov_2.603941, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000400 DAOSGN010000000
VERSION     DAOSGN010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148196_length_1070_cov_2.603941"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PK087_05165"
     CDS             complement(<1..747)
                     /locus_tag="PK087_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18694.1"
                     /translation="MKKKDKFTMPQEKINITIPETPSGKRKPQVHDDEIYFERVVFTN
                     MGNNINKLPDLKSKIAKIKDTLKKFEEKGEIVWHQFYFLKLFSRARGKKAEVINLVHE
                     FDKQVTRMRRILDDLKNKVDLFQYMEKIDDQELEDSYTKVLDLFVFLDDLSKELSSFQ
                     NKYFKKMKMTSYSICNDKNYQELEALNRNIALLLDGFKSFAEAHDYIYYHSGELITNT
                     IKALVHCLETSKLTNYSTTYNYYYFLSTDAV"
     gene            complement(876..>1070)
                     /gene="eno"
                     /locus_tag="PK087_05170"
     CDS             complement(876..>1070)
                     /gene="eno"
                     /locus_tag="PK087_05170"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOH18695.1"
                     /translation="HRSGETEDTTIADIAVALNAGQIKTGSMSRTDRIAKYNQLLRIE
                     DELGSTAVFNGLKALYSIKR"
BASE COUNT          342 a          204 c          151 g          373 t
ORIGIN      
        1 tactgcatcc gtacttagaa aatagtaata gttataggta gttgaataat tagtgagttt
       61 actagtttct aaacaatgaa ccaaggcttt aatcgtattg gtaatcaact caccactatg
      121 gtaataaata tagtcatggg cttccgcaaa cgatttaaaa ccatcaagta ataaagcaat
      181 attacgattg agggcttcta attcctgata atttttatca ttacaaatgc tataagaagt
      241 cattttcatt tttttaaaat atttgttttg aaaactagaa agttctttac ttaaatcatc
      301 taaaaagaca aataaatcca acactttggt atagctatct tccagttctt ggtcatcaat
      361 cttttccatg tactggaata aatcaacctt gtttttcaag tcatcaagga ttcttcgcat
      421 ccgcgtaact tgtttatcaa attcatgaac taagttgatt acttcggctt ttttacctcg
      481 cgctcgcgaa aacaatttaa gaaaataaaa ttgatgccaa acgatttcac ctttttcttc
      541 gaacttcttt aaagtatcct tgattttcgc aatcttcgac ttcaaatcag gcaatttatt
      601 aatattattg cccatattag tgaaaacaac tcgctcgaaa taaatttcat catcatgaac
      661 ttggggtttt cttttcccac tcggcgtttc gggaatcgta atattaattt tttcttgagg
      721 catggtaaat ttgtcttttt tcttcatggc gctacacttc tctactttaa atattattat
      781 accattattc ttcaaaaaaa tgaatacaaa ccagccttat ttagccaaat aaaaaggagc
      841 aattctttaa aaaggaaatg ctccatttaa tttacttatc tcttaatact ataaagagct
      901 ttaagaccgt tgaaaacagc ggttgaacca agttcgtctt caatcctcaa caattgattg
      961 tatttcgcaa tccgatccgt ccggctcatg gaaccggttt tgatttgacc agcattaaga
     1021 gcaacagcaa tatccgcaat cgtcgtatct tccgtttccc cggaacgatg
//
LOCUS       DAOSGN010000401         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_150189_length_1061_cov_2.659046, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000401 DAOSGN010000000
VERSION     DAOSGN010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150189_length_1061_cov_2.659046"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK087_05175"
     CDS             complement(<1..297)
                     /locus_tag="PK087_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOH18696.1"
                     /translation="MQAIKRFNQEGYITYYQSQEKVLIPAVYQEKKQELRGFWFSTVN
                     NIDIPKMTSIAQYQEYLLGVIAKAKEYHFNTIIFQVRPTNDAWYQSKLNPWTSFI"
     gene            complement(284..>1061)
                     /locus_tag="PK087_05180"
     CDS             complement(284..>1061)
                     /locus_tag="PK087_05180"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOH18697.1"
                     /translation="GYLAGSDELRTSDLHEMFLDEEVKAILCLKGGYGCSRLVDKLDY
                     ELIKQHPKLLIGFSDITVLINAIYQQTGLPMMHGAVGIYLGSPSFDASSLDDFAHMLF
                     SPQLGRVLKNPHDDAITLNPGLSSGVLVGGNLSLIAALQGTAWDIPFKNKIVLIEDVD
                     EAPYRLDRFFASLRLSGKLREAVGFVFGYFTGCEPKDPSVWDVNELIDQYFKPLGVPV
                     VKNFASGHNYPFLNLPIGLQVELNADTKTITILEELYASH"
BASE COUNT          337 a          226 c          203 g          295 t
ORIGIN      
        1 aataaaactg gtccacgggt ttaacttaga ttgataccaa gcatcattgg tgggacgaac
       61 ttggaaaata atcgtattaa agtggtattc tttggctttc gcaatcaccc ccagtaaata
      121 ctcttggtac tgagcaatgg atgtcatttt cggaatatcg atgttattga cggtggaaaa
      181 ccaaaaaccg cgcagttctt gcttcttttc ttggtaaaca gccggaatca aaaccttttc
      241 ctgactttga taataggtaa tataaccttc ttgattaaaa cgtttaatgg cttgcatata
      301 attcctccaa aatggtaatg gtttttgtat cggcattcag ttcgacttga aggccaatcg
      361 gtaaattgag aaagggataa ttgtgaccgc tggcaaaatt tttaactacc ggcacgccca
      421 agggtttaaa gtattgatca atcagttcat taacatccca aacggaggga tctttcggtt
      481 cacagccggt aaaataccca aacacaaaac caactgcttc ccgaagctta ccgcttaagc
      541 gtaaactggc gaaaaaacga tccaagcgat acggagcttc atcgacatct tcaatcaaaa
      601 caattttgtt tttaaaaggg atgtcccaag ctgttccttg taaagcggca atcaaactta
      661 aattgccacc cactaaaaca ccggaagaaa ggcccgggtt aagcgtgatt gcatcatcat
      721 ggggattttt taaaacgcgt ccaagttgcg gtgaaaacaa catgtgggca aaatcatcca
      781 aggatgaagc atcaaacgat ggactgccta aataaatccc taccgcccca tgcatcatcg
      841 gtaagccggt ttgttggtaa atggcattga ttaaaacggt aatatcacta aaaccgatta
      901 ataatttcgg gtgttgtttt attaattcat aatccagttt atcaactaag cgcgaacaac
      961 catagccccc tttcaaacaa aggatggctt ttacttcctc atctaaaaac atttcatgaa
     1021 ggtcgctagt tcttagttcg tcactcccgg ctaaataacc a
//
LOCUS       DAOSGN010000402         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_150609_length_1059_cov_3.121514, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000402 DAOSGN010000000
VERSION     DAOSGN010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150609_length_1059_cov_3.121514"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PK087_05185"
     CDS             <1..707
                     /locus_tag="PK087_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOH18698.1"
                     /translation="GNNTKMNPYDLYALETALRFKEVNGAHITVITMGPPSSKAVLEE
                     ALWMGADDAVLLTDRKFAGADVVATSYTLSSGIKTIGAFDLIICGKQTTDGDTAQVGP
                     EIAECLHIPHISYVDKIIKIKDEAVLVSVSMDKTTEQWEITYPGLITVDKGIFTPRLP
                     SYKRSKQLKDEQIKVLTFADFADQDESHYGLKGSPTQVERMFPPQKNTDKEVWTGTNQ
                     ELSQQLFQKMKDLKIL"
     gene            715..>1059
                     /locus_tag="PK087_05190"
     CDS             715..>1059
                     /locus_tag="PK087_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOH18699.1"
                     /translation="MGRIIIDHTKVTKEIGEAAVKICPFNAIEYNGILSINAGCRMCK
                     ICVRKGPAGVFSVEEDVVRAIDKSKWVGVAVFVEHNKHEIHPVVFELIGKAKELAKVI
                     NHPVQAVLFTDDA"
BASE COUNT          318 a          194 c          259 g          288 t
ORIGIN      
        1 atggcaacaa cacgaaaatg aacccctatg atttatatgc tttagaaacg gcattacgct
       61 ttaaggaagt taatggggct catattaccg tgattaccat ggggccacca tcatccaaag
      121 cggttttgga agaagcgctg tggatggggg cggatgatgc ggtgttactc accgaccgga
      181 aatttgctgg ggcggatgtc gtcgctactt cctatacgct ttcttccggg attaaaacta
      241 tcggcgcttt tgatttaatt atttgtggca agcaaaccac tgatggtgat acggcccaag
      301 taggaccgga gattgcggaa tgtttgcaca ttccccatat ttcctatgtt gataaaatta
      361 ttaagattaa ggatgaagcc gttttagtca gtgtcagtat ggataaaact accgaacaat
      421 gggaaattac ctatcctggt ttaattaccg ttgataaggg catttttacg ccacgtttgc
      481 cttcttataa acgcagcaag caattaaaag atgagcaaat taaagtctta acttttgcgg
      541 actttgcgga tcaggatgaa agccattatg gcttaaaagg ttcacccacg caggtggagc
      601 gcatgtttcc gccccaaaag aataccgaca aagaagtatg gaccggaacc aatcaggaac
      661 taagccaaca gttattccaa aaaatgaaag atttgaagat attgtaggac gactatggga
      721 agaataatta ttgatcatac caaagtaacg aaagaaatcg gtgaagcggc tgttaagatt
      781 tgtcccttca atgccattga atataatggc attttaagca ttaatgccgg ttgtcggatg
      841 tgtaaaatct gtgtgcgtaa agggccggcc ggagttttta gtgttgaaga agatgttgtt
      901 agggctattg ataaaagcaa atgggtcgga gtggcggtgt ttgtggagca caataagcat
      961 gaaatccatc ccgttgtctt tgaactgatt ggcaaagcca aggaactggc taaggtaata
     1021 aaccatcccg tccaggcggt gttatttacc gatgatgct
//
LOCUS       DAOSGN010000403         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_150692_length_1059_cov_2.120518, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000403 DAOSGN010000000
VERSION     DAOSGN010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150692_length_1059_cov_2.120518"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1037)
                     /locus_tag="PK087_05195"
     CDS             complement(9..1037)
                     /locus_tag="PK087_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOH18700.1"
                     /translation="MMLSSSTHLMNEALRRVVGEHVYQQGSRVGSDNFHFDFNHYALL
                     TASQIIAIEDLVNQQIQAAHSVVIKEMPLSEAKEMNVQAVFGEKYGDIVRVVNMDFSK
                     ELCGGCHVRNTKDIKQFVILSVESKGSGIYRIEATSGTNAEYELQKTVVNIVNEIDDL
                     RLKIHDLNVKAAEEKINFEVSAIPRTPFVVSYRYIVKLREESMLLREQLKELEKHYVK
                     FKKDAFVIDLDSFLNQVINIQSYRILITKTAFLEIDHAKDAVDRFADKLGESLIFFAN
                     LINERLVFICKSKIAALHAGNLVKMAATFTNGNGGGKSDFAQAGGKDLSKVDDALVLI
                     KQEVVSKL"
BASE COUNT          347 a          218 c          167 g          327 t
ORIGIN      
        1 caacaatttc ataatttact gactacttcc tgcttgatta acaccaaagc gtcatcaact
       61 tttgataaat ccttccctcc ggcttgcgca aaatcgcttt tgccaccacc attaccattg
      121 gtaaaggtcg ctgccatttt cacaagattt cccgcatgca aagcggcgat tttactctta
      181 caaataaaga caaggcgttc attaatcaaa ttagcaaaga aaatcaggga ttcaccaagt
      241 ttatcagcaa agcgatcaac ggcatcctta gcatgatcaa tttccaaaaa ggctgttttc
      301 gtaattaaaa tacgataaga ttgaatatta attacttgat taaggaagga gtccaaatca
      361 attacgaaag catctttttt aaatttcaca tagtgttttt ctaattcttt taactgttct
      421 ctaagaagca tagattcttc ccgaagtttg acgatataac gataactaac aacaaacgga
      481 gtccttggaa tggcagaaac ttcgaaattg attttttctt cagcagcttt gacatttaaa
      541 tcatgaattt ttaaccgcaa atcatcaatt tcgttcacaa tattgacaac agttttttgt
      601 aattcatatt cggcattagt ccccgaagta gcttcaattc ggtaaattcc cgagccctta
      661 ctttcaacgg ataaaataac aaattgttta atgtctttag tgttacgaac atggcagcca
      721 ccgcataatt ctttagagaa atccatgttg acgacacgaa caatatcacc atatttttca
      781 ccgaatacag cttggacatt catttctttg gcttcactaa ggggcatttc cttaataacg
      841 accgaatgag ctgcttggat ttgttgatta actaaatctt caatagcgat aatttgtgaa
      901 gcggtaagca aagcatagtg gttaaagtca aagtgaaagt tatcgctacc aactctggac
      961 ccttgttgat aaacatgttc cccgacaacc cgacgcaaag cttcattcat taaatgggtg
     1021 gatgaagaca gcatcatcaa aattatatta aacacctat
//
LOCUS       DAOSGN010000404         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_151168_length_1057_cov_1.737525, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000404 DAOSGN010000000
VERSION     DAOSGN010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151168_length_1057_cov_1.737525"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..981
                     /locus_tag="PK087_05200"
     CDS             61..981
                     /locus_tag="PK087_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18701.1"
                     /translation="MIALAIPLAIALKMSSDEDYPLAESQALYDSSDEVYGFIATSVV
                     AMIYSTNDVFAGENLDKQGNSDLLVFNELSFLNRYLSPIEIMLAYDHNHFYRQASDNR
                     YYTNKIVYDGIGLLDENLHYDIYYNEYLVANNRLEINGFVRAEAKTYTLKGSKEIQNQ
                     KTILQMTYYINEDSTNDYVRVSKNLNVGLDEYEFVVVKDGLVLNESKLSLTNGQEIYA
                     TLENLLEKTATFKIYASKENTFDIDYTIAVATPISYEPYMNEGDGKNISSEPFIESGK
                     IAFDVEKNSSSYFISSNDVEDVIIINNPHD"
BASE COUNT          343 a          203 c          182 g          329 t
ORIGIN      
        1 cgctagttcc caaaaaaatg gcttaccttc tcttggcgag tagccctaag ctctatcttg
       61 gtgattgccc ttgccattcc cttagcgatt gccttaaaaa tgtcttccga tgaagactat
      121 ccgcttgctg aaagccaggc cttatatgat tcatccgatg aagtctatgg atttattgca
      181 acttccgttg tcgccatgat ttacagcacc aatgatgtat ttgccggtga aaacttagat
      241 aaacaaggaa atagtgattt attagttttt aacgaactta gtttccttaa tcgttatcta
      301 tcaccgattg aaatcatgct ggcctacgac cacaaccact tttaccggca agccagtgat
      361 aatcgttatt atactaacaa aattgtttac gacggcatcg gtttattgga tgaaaatctc
      421 cattatgaca tttattacaa tgagtattta gtcgccaaca atcgcttgga aataaatggt
      481 tttgttcgtg ctgaagctaa aacttacact ttaaaaggca gcaaagaaat ccaaaaccaa
      541 aaaaccatcc tccaaatgac ttattacatc aatgaagatt caacgaatga ttatgttcga
      601 gtctccaaaa acttaaatgt tggcttagat gaatacgaat ttgtggttgt gaaagacggt
      661 ttagtgctaa atgaaagtaa attatcctta acgaatggcc aagagatata tgcaacttta
      721 gaaaacttgc tggaaaaaac ggcaactttc aaaatctatg cttcaaagga aaacaccttt
      781 gatattgatt atacgattgc ggtggcaaca ccaattagtt atgaaccgta tatgaatgag
      841 ggtgatggca agaatatctc ttccgaacca ttcattgaat caggaaagat tgcctttgat
      901 gttgagaaaa acagttcatc ctattttatc tcatctaatg atgtcgaaga tgtcatcatt
      961 atcaacaatc cccacgatta agatctatgg aaaacgacac cttaaaagat gtcgtttttt
     1021 tgtttcctat tcttactatt atgaaaaacc ttttcct
//
LOCUS       DAOSGN010000405         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_152203_length_1052_cov_3.560682, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000405 DAOSGN010000000
VERSION     DAOSGN010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152203_length_1052_cov_3.560682"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PK087_05205"
     CDS             complement(<1..787)
                     /locus_tag="PK087_05205"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOH18702.1"
                     /translation="MPFIKEVYAREVLDSRGNPTVEVEVYTESEAFGRAIVPSGASTG
                     EHEAVELRDGDKKRYLGKGVLNAVDNVNDVIAPEIIGMNVFDQVGIDKLMLELDGTAN
                     KGKLGANAILGVSMAVARAAAELSGLSLYNYLGGFNAKELPTPMMNILNGGSHADNNV
                     DFQEFMIMPVGAPSFKEALRMGTEVFHHLKKVLKARGMNTAVGDEGGFAPNLSSNEEA
                     IIVILEAIKNAGYVPGKDVVLAMDVAASEFYHKESKTYVLDGEG"
     gene            complement(793..>1052)
                     /locus_tag="PK087_05210"
     CDS             complement(793..>1052)
                     /locus_tag="PK087_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018660883.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOH18703.1"
                     /translation="ITDLGGTLMIIADHGNAEKLVDELGKPFSAHTTNPVPCIITKKG
                     LKLRQGGILADVAPTMLQLMNLPQPPVMTGKSIIESFEERK"
BASE COUNT          300 a          264 c          183 g          305 t
ORIGIN      
        1 cgccttcacc atcaagcaca taagttttgc tttccttgtg ataaaattcc gaagcggcca
       61 catccattgc taaaacaaca tccttacccg gaacataacc ggcattcttg atggcttcca
      121 aaataacgat gatggcttct tcattgctgg aaagattagg agcaaaacca ccttcatcac
      181 caacagcggt attcatccca cgcgctttta agactttttt aagatggtgg aagacttccg
      241 ttcccattct taaagcttct ttaaacgaag gtgcaccaac gggcataatc ataaattctt
      301 ggaaatcgac attgttgtca gcgtgagaac caccatttaa aatgttcatc atcggtgtcg
      361 gtaattcttt cgcattgaat ccgcctaaat aattgtataa ggacaaaccg cttaattccg
      421 ctgcggcccg ggccaccgcc atcgaaacac ctaaaatggc attggcgcct aattttcctt
      481 tgtttgctgt gccgtcaagt tccagcatta atttgtcaat cccgacttga tcaaaaacat
      541 tcatcccgat aatttccgga gcaataacat cattaacatt gtcaacggca tttaagacgc
      601 cttttcctaa ataacgcttt ttatcgccat cacgcaattc aacggcttcg tgttcaccgg
      661 tcgaagcccc actaggaaca atggcccgcc caaaggcctc actttcggta taaacttcca
      721 cttcaacggt gggattccca cgagagtcca atacttcacg ggcataaact tctttaataa
      781 atggcataat ttttatttcc tttcttcaaa tgattcaata attgattttc cagtcatcac
      841 tggtggctgt ggtaaattca tcaattgcaa catggtcgga gccacatcag ctaaaatacc
      901 accttgacgt aattttaaac cttttttcgt aataatacaa ggaacgggat ttgtggtatg
      961 agcagaaaac ggttttccca attcatcaac cagtttttcc gcattaccat ggtcggcaat
     1021 aatcataagc gtccccccaa ggtccgtgat tt
//
LOCUS       DAOSGN010000406         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_152968_length_1049_cov_2.553320, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000406 DAOSGN010000000
VERSION     DAOSGN010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152968_length_1049_cov_2.553320"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PK087_05215"
     CDS             <1..781
                     /locus_tag="PK087_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOH18704.1"
                     /translation="LFPYSNQNKLAPKGTYLPAKMFYGRDYHLVVKDKLQEFVVKWEL
                     PDAVIGVDTSPLDERLCAYLAGLGTFGDHNLLINPTLGTFFAIGTIMTSAVFHEYQQP
                     IAELCTHCGACLKACPTQALTGGFHKERCLSNLSQKASSDFSLYDLMHHTCYGCDLCQ
                     DACFYNSHPDYSLPELAWDGLSVLTWEGFLQLDAKQYFDYYRDKSWSWIAYERMLRNL
                     MVLRHHDQALSEQEVIQLATKTTKPWFLKHLQYLRGQHGKS"
     gene            768..>1049
                     /locus_tag="PK087_05220"
     CDS             768..>1049
                     /locus_tag="PK087_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOH18705.1"
                     /translation="MGNLNIVLFEPEIPQNTGNIMRTCIASNTVLHLIEPLGFKLSDR
                     AIKRSGVNYISEVKYFVYKNLDDFFEKNTGRYLYLTRYAQQNFFDCHLTD"
BASE COUNT          292 a          198 c          213 g          346 t
ORIGIN      
        1 cttgtttcct tattccaacc aaaataaatt agctcctaaa ggaacatatc ttcctgccaa
       61 aatgttttac ggaagggatt atcacttggt tgttaaagat aaattgcaag aatttgtggt
      121 gaagtgggaa cttcctgatg ccgtcattgg tgtggacacc agccccctcg atgaaagatt
      181 atgtgcttat ttggcgggct tagggacttt tggcgatcat aatttgctga ttaatcccac
      241 tctcggcact ttttttgcca ttgggacgat tatgaccagt gcggtttttc atgaatatca
      301 acaaccaata gcggaattat gtacccattg cggggcctgc ttaaaggctt gtccgacaca
      361 ggcacttaca ggcggttttc acaaagaacg ctgtttatcg aatttatcgc aaaaggctag
      421 tagtgatttc tctctttatg atttaatgca tcatacttgc tatggttgtg atctttgcca
      481 agacgcctgc ttttataact cccatcccga ttattccttg ccggaattag cttgggacgg
      541 tttatcagtg ctgacttggg agggtttttt acaacttgat gctaagcaat actttgatta
      601 ttatcgcgat aagagttgga gctggattgc ttatgaacgg atgttacgta atctcatggt
      661 attaagacat cacgatcaag cacttagtga gcaagaagta atccaattag ccaccaaaac
      721 cactaaacca tggtttttaa agcatttaca atatttacga ggtcaacatg ggaaatctta
      781 atatcgtttt gtttgaaccg gaaattcccc aaaatacggg taatatcatg cggacctgta
      841 ttgctagcaa tacggtttta catttaattg aacctctcgg ctttaaactt agtgatcggg
      901 ccattaaaag gtccggggtc aactatattt ctgaagtaaa gtattttgtt tataaaaatt
      961 tggatgattt ttttgaaaaa aataccggtc gctatttata tttgactcgt tatgcccaac
     1021 agaacttttt tgactgtcat cttacggat
//
LOCUS       DAOSGN010000407         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_153154_length_1048_cov_2.911380, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000407 DAOSGN010000000
VERSION     DAOSGN010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153154_length_1048_cov_2.911380"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1048
                     /locus_tag="PK087_05225"
     CDS             <1..>1048
                     /locus_tag="PK087_05225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOH18706.1"
                     /translation="RDITEIKKVEEESLRMEMQWDKLIQEMPLGFNLREMIWDEKKNP
                     VDYRFLDINDAYQAMTGLKKADIIGKRATEILPEIEFSWIESYAQVVLKKQTTAIESY
                     SRALKKYFRVVSYPYIDDKFIVIAEDITDRKHQEKALIEKESEKGRIIDNLPGVYYQC
                     KFDNNRTMLHMTEQWERITGYKPVDLINNKRISFNDLVLPKYRTHLMKTWLESKKHNR
                     TNTLEYEILKKDGTTAWIWEKGKAFSRDGEWFIEGFLMDNTDQKQNEEKVIYASKHDF
                     LTGLPNRRYFDDKIKEFDKPENYPLLIAVIDIDGLKLINDTYGHHVGDQTIKQVANLL
                     AESWLPPAIISRIGG"
BASE COUNT          376 a          164 c          230 g          278 t
ORIGIN      
        1 agagatatta ctgaaatcaa aaaagttgaa gaagaaagcc tacgaatgga aatgcaatgg
       61 gataagttaa tccaagagat gccactgggc tttaatttaa gagaaatgat ttgggatgaa
      121 aagaaaaacc cggtggacta ccggtttctt gacatcaatg atgcttacca agcgatgacg
      181 ggcttaaaaa aagcggatat cattggtaaa cgagcaaccg agattcttcc ggaaattgag
      241 ttttcgtgga ttgaaagcta tgcacaagtc gtgttaaaaa aacaaaccac agcgattgag
      301 tcttattcgc gggctttaaa aaaatatttt cgggtggttt catatccata tattgatgat
      361 aagttcattg tgattgcgga agacattacg gatagaaagc atcaggaaaa agccctcatt
      421 gagaaagaaa gcgaaaaagg gcggattatt gacaacctcc ccggagttta ttatcaatgt
      481 aagttcgata acaataggac aatgctccat atgactgagc aatgggaaag aatcaccggc
      541 tataaacctg tggatttgat aaacaataaa agaatatcat ttaatgattt agtccttcct
      601 aagtatcgga ctcatttgat gaaaacttgg ttggaatcaa agaaacataa tcgaacaaat
      661 acccttgagt atgaaatcct caagaaagac ggaacaacag cttggatttg ggaaaaaggg
      721 aaagccttta gtcgagatgg agaatggttt attgaaggct tcttaatgga taatacggat
      781 caaaaacaaa atgaagaaaa agtgatttat gcaagtaaac atgatttttt aacgggctta
      841 cccaatagac gctattttga tgacaaaatt aaagaatttg ataaaccgga aaactatccc
      901 ttattgattg ctgtgattga tattgatggc ttgaagttga ttaacgatac ctatgggcat
      961 catgtcggtg atcaaaccat caaacaagtt gctaacttgc ttgccgagtc gtggttaccc
     1021 cccgccatta tttcaaggat tggtgggg
//
LOCUS       DAOSGN010000408         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_153401_length_1047_cov_2.401210, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000408 DAOSGN010000000
VERSION     DAOSGN010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153401_length_1047_cov_2.401210"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PK087_05230"
     CDS             complement(<1..255)
                     /locus_tag="PK087_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOH18707.1"
                     /translation="MFIGEFHFNIDDKGRLFIPSDLRKDLGIEVVVARGIEACLYVYP
                     LEAWKVIADKLSSLSFTKKNNREFSRLFLSGAYQKDIDTKG"
     gene            complement(604..768)
                     /gene="rpmF"
                     /locus_tag="PK087_05235"
                     /pseudo
     CDS             complement(604..768)
                     /gene="rpmF"
                     /locus_tag="PK087_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
     gene            complement(788..>1047)
                     /locus_tag="PK087_05240"
     CDS             complement(788..>1047)
                     /locus_tag="PK087_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18708.1"
                     /translation="HLFDDCFTFALHIVGNMTLEDARTLDPVIFPIDIHITETFNTQL
                     FDDDSRLIEKNTVDLRPVVWEDIYLEKPMRVVKEEIQNNQN"
BASE COUNT          304 a          210 c          152 g          381 t
ORIGIN      
        1 tcctttagtg tcaatatctt tttggtaagc acccgataaa aacaaacgac taaattcacg
       61 attgtttttt ttcgtgaagg aaagtgaaga cagtttatca gcgattactt tccaagcctc
      121 aagcggataa acatataaac aggcttcaat cccacgagca acaacaactt caatccccaa
      181 gtcttttcgt aaatcactag gaataaatag tcgtcctttg tcatcaatat taaaatggaa
      241 ttcaccgata aacataattt cctccacttt tccccacttc gccccacaaa tatgataaca
      301 taccttattt tttaatgcaa gagctttttt aaaaatattt taaaaaaatt aaaaagcact
      361 caattttgag tgcttttcat caagtaaaaa tttagaatga tgtcgttttt actttccttc
      421 aggttttttg gctgctttaa tcggggattt tacttgagat tttgtctctt ttgtaacttt
      481 cccagcacct tttttggcat cttttaaagc ttttacctct ttacgagcag ttttagtttt
      541 ggcttctgct tttgtttcag cggcttcttc tttttctgct gtcttcttaa ctttaatctc
      601 ttttacaagt tttccatcat agtaaccaca ttctttacaa accgtgtgag ctaatttcat
      661 ttctccacaa ttagggcaaa ccatcattcc tggtaactct aatttaaaat gggagcgacg
      721 aaggcgtttt ctagttttgg aagttcttct ctgagggaca attgccattt cttctattca
      781 cctcttttta gttttgatta ttttggattt cttctttcac aactcgcatt ggtttttcca
      841 aatagatatc ttcccaaaca acgggccgta aatcaaccgt gtttttttca attaaccggg
      901 aatcatcatc aaataattgc gtattaaatg tttcagtaat atggatgtca atggggaaaa
      961 ttaccgggtc caaggtcctg gcatcctcta aagtcatatt tccaacaatg tgaagagcaa
     1021 aagtaaaaca atcatcaaat aaatgtt
//
LOCUS       DAOSGN010000409         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_153873_length_1045_cov_2.123232, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000409 DAOSGN010000000
VERSION     DAOSGN010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153873_length_1045_cov_2.123232"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..762)
                     /locus_tag="PK087_05245"
     CDS             complement(121..762)
                     /locus_tag="PK087_05245"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOH18709.1"
                     /translation="MKLIAMPLKMFLDEIDAPTAIPGGGSAAAFGVAMGISLLGMVGK
                     LTMTKKKFLQLDEKVKDDFYKQLSTLGALKQEALTLVESDAGSYQQIMEAYRLPKESD
                     EHIAIRKQAIDQATIHATEVPLLTAKTAFTALEIALPLFPVIVKSTGSDFGVAVLMLE
                     AGLVGAVFNVRTNMAGFMDAKIADTYLTKAKEYEEKGKAIAKQALFLVNELWK"
     gene            complement(775..>1045)
                     /locus_tag="PK087_05250"
     CDS             complement(775..>1045)
                     /locus_tag="PK087_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH18710.1"
                     /translation="TSPSENLQLAMQMAFLKGKLTPEEIITAVTINAAVSLGLEKRKG
                     SFEVGKDADLVFLKIPNLAYLLYHFGINHVHSVYIGGQKLIERET"
BASE COUNT          297 a          255 c          175 g          318 t
ORIGIN      
        1 taaaaaaacc ggcaaggatg gcagaaataa gtaatttccc caacggtttt cggactttat
       61 tttcccccac tttaatcgct tcctcaacaa cttcttctgg agaatacaac aaatgattgt
      121 ttatttccat aattcattca ccaaaaacaa agcttgctta gctatcgctt ttcccttttc
      181 ttcatattcc ttagctttag ttaaataagt atccgctatc ttagcatcca taaaaccggc
      241 catattagtg cgcacattga aaactgcccc caccaaaccc gcttccaaca tcaacacagc
      301 gaccccaaaa tccgagcccg tgcttttcac aatcaccgga aacagcggta aagcgatttc
      361 caaagcggtg aaagccgttt ttgccgttaa cagcgggact tccgtggcat gaatagtcgc
      421 ttggtcgatg gcttgttttc ggatagctat gtgctcatcg ctttctttcg gtaagcggta
      481 agcttccata atctgctgat aagaaccagc atcactttca accaaagtta aagcttcctg
      541 ctttaaagcg cccaatgttg atagttgttt ataaaagtca tctttcacct tttcgtcaag
      601 ttgaagaaat ttctttttcg tcatggtcag ttttccaacc attcccaata aactaatgcc
      661 catggcaaca ccgaaggcgg cagcacttcc gccaccggga atcgctgttg gcgcatcaat
      721 ttcatctaaa aacattttca ggggcatggc aattaatttc atcttgttct ccttttatgt
      781 ttctctttca atcagctttt gaccgcctat ataaacacta tggacatgat taatcccaaa
      841 atggtagagc aaataggcta aattaggaat tttaagaaaa accaaatcgg catcttttcc
      901 gacttcgaaa gagcccttcc ttttttctaa acctaaacta acagccgcat tgatggtaac
      961 agcggtgatg atttcttccg gtgttaattt tccctttaaa aaagccattt gcatcgccaa
     1021 ctgcaaattt tctgaaggac tggtt
//
LOCUS       DAOSGN010000410         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_155605_length_1038_cov_1.899288, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000410 DAOSGN010000000
VERSION     DAOSGN010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155605_length_1038_cov_1.899288"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PK087_05255"
     CDS             <1..777
                     /locus_tag="PK087_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOH18711.1"
                     /translation="KCLQQYVNTIENKQLRQAIDYALFPGGKRLRPLLLLLTLADFGI
                     NQEMGIYIAVALEMIHTYSLIHDDLPAMDDDDFRRGKASLHKQFSEAVAILAGDALLS
                     DAFGFITKSPLEATTITKIVALISQKTGSNGMVQGQMMDISDNHLLNEQFITLMNSRK
                     TGDLFMVALQGAGLIAKVDEKFYLQLGEIALSLGLAFQIKDDLDDYSHEQAFEPNTFV
                     ALFGQAKARDLFIYYRELTFERIKSYLGENNLYDFAKRIL"
     gene            774..>1038
                     /locus_tag="PK087_05260"
     CDS             774..>1038
                     /locus_tag="PK087_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOH18712.1"
                     /translation="MINIEQITCPSDIKKMNIKELEQLAGQVRQFIIENVSKTGGHLS
                     ANLGVVELSIALHYVFNSPHDKLIFDVGHQTYTHKILTGRSAQF"
BASE COUNT          325 a          167 c          218 g          328 t
ORIGIN      
        1 aagtgtttgc agcaatatgt aaacactatc gaaaataagc aactgcggca agcgattgac
       61 tatgccttat ttcccggagg aaaacgcctt cgtcctttgt tattgctgtt aactttggcg
      121 gatttcggaa ttaatcagga aatgggaatt tatatagctg ttgctttgga aatgattcat
      181 acatattcat taattcatga tgatttacca gctatggatg atgatgattt tcgtcgaggt
      241 aaagcaagtc tccataaaca attttcggaa gcggtggcca ttttggcggg cgatgctttg
      301 ttgtccgatg cttttgggtt tataaccaaa tcgcctttgg aagcgacaac aataactaaa
      361 attgtggctt taatatcgca aaaaaccggt agtaacggga tggttcaagg tcaaatgatg
      421 gacatttccg ataatcatct tttaaatgaa caatttatta ctttaatgaa ttcacggaaa
      481 accggtgatt tattcatggt ggctttgcaa ggagcggggt tgattgcgaa ggtggatgag
      541 aagttctatc ttcaattagg agaaattgct ctatctttgg gtttggcttt tcaaattaaa
      601 gatgatcttg atgattattc ccacgagcaa gcttttgaac caaatacttt tgtggccttg
      661 tttggtcaag ccaaagccag agatttattc atatattacc gagaactaac ttttgaaaga
      721 attaaaagct atttaggaga aaataatctt tacgattttg caaagaggat tttatgatta
      781 acattgaaca gataacttgc ccatcggaca ttaaaaagat gaatattaaa gaattagaac
      841 aattagcggg gcaagttcgc caatttatta ttgagaatgt cagcaaaacc ggtggacatt
      901 tgtcagctaa tttaggggtt gtcgaactaa gcattgcttt gcactatgtt tttaacagcc
      961 ctcatgataa attaatcttc gatgtcggac atcaaactta cacccataaa atattaacgg
     1021 gacgaagcgc gcaatttg
//
LOCUS       DAOSGN010000411         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_156002_length_1036_cov_2.327217, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000411 DAOSGN010000000
VERSION     DAOSGN010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156002_length_1036_cov_2.327217"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PK087_05265"
     CDS             <1..733
                     /locus_tag="PK087_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOH18713.1"
                     /translation="ISPAAMNNFQIAVNFASLLAIIIYYRHLLKELIVGCWQFLFKKN
                     KDQKPAFLYLVYIILATIPAGIVGLIIKLMDWDQYFTNILVVGISLLITGSLLMWIAK
                     KQTTALRETITWKDALLMGGAQVVGLLPGISRSGITSAVGVSNKLQLEKALRFSFMMY
                     IPASIAAAFLGVYDLIKPDTTATEPVFWLGYLGGFLASLISTYIAITVFFKLVKHKNI
                     KYFGFYCLAVATIVLILIATGVFKW"
     gene            727..>1036
                     /locus_tag="PK087_05270"
     CDS             727..>1036
                     /locus_tag="PK087_05270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH18714.1"
                     /translation="MVKYKICKTKNQIIDHFWVRKTVFVEEQKVTMEEEFDLEEKNHT
                     MFVVYDNHHPIGAARLKIMGHQAKIQRVCILKTYRFLGYGYSLMMELHKYCQAYGVSE
                     I"
BASE COUNT          288 a          210 c          210 g          328 t
ORIGIN      
        1 tatttccccg gcagcgatga ataattttca aattgctgtg aactttgctt ccctattagc
       61 aattattatt tattaccgac atttgcttaa agaattgatt gttggttgtt ggcaattcct
      121 ttttaaaaaa aacaaagacc aaaagccggc ctttctctat cttgtttata tcatcctagc
      181 caccattccg gcgggtatag tcggtttaat catcaagtta atggattggg atcaatactt
      241 tactaatatc ctagtggtgg ggatttccct tttaataacc ggttcactat tgatgtggat
      301 tgcgaaaaag caaaccaccg ccctaagaga gaccattact tggaaagatg ctctcttgat
      361 ggggggcgca caagtggtcg gtttgttgcc ggggattagc cgttccggca ttacttccgc
      421 cgtcggtgtt agcaataagc tgcaactgga aaaagccttg cgtttttctt ttatgatgta
      481 tatccccgcc agtatcgcag ccgctttcct tggggtttac gatttaatta aacccgatac
      541 gacagccacg gaaccggtgt tttggcttgg ttaccttggc ggctttcttg ccagccttat
      601 tagcacttac atcgccatta ccgtcttttt taaattggtc aaacataaaa atatcaagta
      661 tttcggcttt tattgtttag ccgtcgctac cattgtttta atcctcatcg ctacgggagt
      721 ctttaaatgg taaaatataa aatttgtaaa acaaagaatc aaatcatcga ccatttttgg
      781 gtccgaaaaa ccgtgtttgt tgaggaacaa aaagtaacaa tggaagaaga gtttgacctg
      841 gaagaaaaaa accacaccat gtttgtggtc tatgataatc atcatccaat cggtgcggcc
      901 cgccttaaaa tcatgggcca ccaagccaaa attcaacgtg tttgcatttt aaaaacttat
      961 cgcttcttag gatacggcta tagtttaatg atggaactgc ataaatattg ccaggcttat
     1021 ggggtttcgg aaatta
//
LOCUS       DAOSGN010000412         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_156006_length_1036_cov_2.319062, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000412 DAOSGN010000000
VERSION     DAOSGN010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156006_length_1036_cov_2.319062"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1036
                     /locus_tag="PK087_05275"
     CDS             <1..>1036
                     /locus_tag="PK087_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOH18715.1"
                     /translation="KVRGLIAHIQLLKKNPSSPGHFFSNEEFFKSIFNTTTAALFVID
                     LEGNIKFCNLICRRLLGYERESDLLGKNAHLLFHHHNVLNQIIPVEECGITQVFKTKA
                     GFYSDKEVFWRKNRTSFSVEVFAHPLFQQGQITGVVVTFFDITRRKAVEKQLKESVHS
                     HSVLLSNLPGMAYKCNFDRNWTMQFVSEGCFELTGHRPENIINNRDLSFNELIAPEFQ
                     EHLWQVWGDAVKNRQAVREEFVIVTATGERKWVLEQGQPVYNEMGFVESLEGLILDIS
                     EQKSKQIEIQYMSEHDMLTGLYNRGYFDKERARLDHLQYLPLSIIVADINGLKLTNDA
                     FGHDEGDYLIK"
BASE COUNT          317 a          188 c          214 g          317 t
ORIGIN      
        1 aaaagtaagg ggtttaattg ctcatattca attgttaaag aaaaacccat catcccccgg
       61 tcattttttc agcaacgaag aattttttaa atccattttt aataccacca ctgccgccct
      121 ttttgttatt gatttggaag gaaatattaa attctgtaat ctcatttgtc gtcgcttgct
      181 tggttacgaa cgggaaagtg atttgcttgg caaaaatgcc catttactct tccaccacca
      241 taatgttctc aatcaaataa ttcccgttga agaatgtgga attacacaag tctttaaaac
      301 caaagccggt ttttactcag acaaagaagt attttggcgg aagaaccgca cgagtttcag
      361 tgttgaagtt tttgctcatc cccttttcca acaaggtcaa attaccggcg tcgtggttac
      421 tttctttgat attacgcgac gtaaagccgt tgaaaaacag ttgaaagaaa gtgttcacag
      481 tcatagtgtt ttgttatcta atttgccggg aatggcttat aaatgcaatt ttgatcgcaa
      541 ttggacgatg caatttgtgt cggaaggatg tttcgaatta accggccatc gcccggaaaa
      601 catcatcaat aatcgggatc tttcgttcaa tgaattaatt gcccccgagt ttcaagaaca
      661 tttgtggcaa gtgtggggtg atgccgtcaa aaatcggcaa gcagtgcggg aagagttcgt
      721 cattgtcacc gccactggag aacgaaaatg ggttcttgaa caagggcaac cggtttataa
      781 tgaaatggga tttgtggaat ctttagaagg acttatcctt gacattagtg aacaaaaaag
      841 caaacaaatt gaaatccaat atatgagtga acatgatatg ttgacagggc tttataatcg
      901 ggggtatttt gataaagaac gcgcccgctt agaccatcta cagtatttac cattgtcgat
      961 tattgttgct gatatcaatg gtttaaaact aaccaatgat gcctttggtc atgatgaagg
     1021 tgattactta ataaaa
//
LOCUS       DAOSGN010000413         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_156177_length_1035_cov_3.427551, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000413 DAOSGN010000000
VERSION     DAOSGN010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156177_length_1035_cov_3.427551"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..>1035)
                     /locus_tag="PK087_05280"
     CDS             complement(42..>1035)
                     /locus_tag="PK087_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOH18716.1"
                     /translation="PTDVDNFSILSEIALSIIAFSIGSEFVMRDMLKVGKKIFWITLL
                     EVIGAIGVVFFVMFVLFKQPFAFSIVIASMSAATAPAATLMVIRQYRAYGPLTKTILP
                     VVALDDVLGIVAFGIALAVAKLTVSAEPVKVADMFLKPLIEVGGSFILGFILGVILSL
                     VSKKAENRDDHQILSIAAIGVATGISKWLGFSPLLTNIVLGTVVVNMVRKSHRVFGSV
                     NDFASPFFVIFFTVAGASLDLAILKTVGLVGLAYILARAGGKFLGSYVGAVVTKSEKT
                     VQKYLGFAMLPQGGISIGLSVLVRQQLPEYAMAITTIIMFSVLIYETTGPIYKR"
BASE COUNT          359 a          260 c          190 g          226 t
ORIGIN      
        1 caggtaacag agccatggtg accagcctgt caacatggat atcaccgctt atatatcggc
       61 cccgttgttt catagattaa aacactgaac ataataatcg tcgtaatcgc catggcatat
      121 tcggggagtt gttgtctgac caagacggat aaaccgatgg aaattccgcc ttgaggtaac
      181 atcgcaaaac ctaagtattt ctggaccgtc ttttcggact tcgttaccac ggccccgaca
      241 taactaccta agaatttgcc accggctctt gctaaaatat aagcaagacc gactaaaccg
      301 accgttttta aaatggctaa atccaaactg gcaccggcaa cggtaaaaaa gataacaaag
      361 aagggggaag caaaatcgtt aactgagcca aaaacgcggt gcgatttacg aaccatattg
      421 acaacaaccg ttcctaaaac gatattcgtt aacagcggcg aaaagcccaa ccatttcgaa
      481 attcccgtcg ccaccccaat cgccgcaata cttaaaatct ggtgatcatc acgattttcg
      541 gcttttttgg aaaccaaaga taaaatcact cctaggataa agcctaaaat aaaagatcca
      601 ccaacttcaa tcaagggttt aagaaacata tcggctactt taacgggttc agcggaaacc
      661 gttaacttcg ctaccgccaa agcaattcca aaagcgacaa tcccaagtac atcatccaaa
      721 gcgacgaccg gcaaaatggt cttcgtcagc ggtccataag cccggtactg ccgaatgacc
      781 atcagtgttg ccgccggagc agttgccgcg gacatggaag caatcacaat cgaaaacgca
      841 aacggttgtt taaacaaaac aaacataaca aagaaaacaa ccccaatcgc cccaatcact
      901 tccaataaag taatccaaaa aatcttcttt ccaactttaa gcatatctct cattacaaat
      961 tccgaaccga tggaaaaagc aataatcgat aaggcaattt cgctaagaat ggaaaagtta
     1021 tcaacatccg tcggt
//
LOCUS       DAOSGN010000414         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_156914_length_1032_cov_2.235415, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000414 DAOSGN010000000
VERSION     DAOSGN010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156914_length_1032_cov_2.235415"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PK087_05285"
     CDS             <1..630
                     /locus_tag="PK087_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOH18717.1"
                     /translation="SLILKTGSHYQKREFTVEGDFSNSACWLAAAYFNPQLRVSGLNP
                     LSRQGDARFFKFLEKMKVKYRYEDGDYFYLNGKIASAEIDISETPDLAPILAAVAALG
                     TGTVIISGIDKLQYKESNRALAIMEGLNRLGGSVEIIDNRMVIHGKALLDGGCEVDSY
                     NDHRMVMAFAILGNQVRSPLIIKNFAAVNKSYPNFFNVFQQIGGKTEVV"
     gene            630..>1032
                     /locus_tag="PK087_05290"
     CDS             630..>1032
                     /locus_tag="PK087_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOH18718.1"
                     /translation="MQTLITNYSRKQVKIYLEDGLISRLTAFLNPNLRYFIITDERVA
                     SLYLDTVINQLPNFDLYLLPSGESSKNLEEVNRIINYMLLADIQKNDYLLALGGGVIG
                     DVTAFVAAIYKRGINYLSVPTTLISQVDSCLG"
BASE COUNT          299 a          179 c          221 g          333 t
ORIGIN      
        1 tctttaattt taaaaactgg tagtcattat caaaaacggg aatttactgt tgaaggtgat
       61 ttttctaatt cagcttgttg gctggctgcg gcttacttta acccgcagtt acgggttagt
      121 ggtcttaatc cactatcacg acaaggtgat gcccggtttt ttaagttcct cgaaaagatg
      181 aaagtaaaat atagatatga ggacggcgat tatttttatt taaatggaaa aatcgccagt
      241 gctgaaattg atatctcaga aacgcctgat ttggccccaa ttttagccgc cgttgctgct
      301 ttagggacgg ggacggtcat cattagcgga atcgacaaac tccaatacaa agaaagtaat
      361 cgagcgcttg caattatgga gggtttgaac cgccttggcg gaagtgttga aatcattgat
      421 aatcggatgg ttattcacgg caaagccttg ttagatggtg gttgtgaagt tgatagttat
      481 aatgatcatc ggatggtgat ggcttttgcc attttaggga accaagtccg ttcaccatta
      541 ataatcaaaa attttgctgc tgtaaataaa tcatatccta atttctttaa tgttttccaa
      601 caaattggcg gaaaaaccga ggtggtttaa tgcaaacctt aataactaac tattctagaa
      661 aacaagttaa aatctattta gaagacggtt taatcagtcg cttgaccgcg tttttaaatc
      721 ccaatcttcg ctattttatc attacagatg aacgcgtggc ttcattatat ttagatacgg
      781 taattaacca actccccaat tttgatcttt atcttttgcc ttctggggaa tccagtaaaa
      841 atcttgagga agtaaatcgg attattaatt acatgctatt ggcggatatc cagaagaacg
      901 attatttatt agcccttggt gggggcgtaa ttggcgatgt aacggcattt gtggcggcca
      961 tttataaacg gggtattaat tatctttccg tcccaacaac tttgatttcg caagtcgaca
     1021 gttgcttggg tg
//
LOCUS       DAOSGN010000415         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_156989_length_1032_cov_1.747185, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000415 DAOSGN010000000
VERSION     DAOSGN010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156989_length_1032_cov_1.747185"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..698
                     /locus_tag="PK087_05295"
     CDS             258..698
                     /locus_tag="PK087_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HOH18719.1"
                     /translation="MNGMTYQEIIDKYGGFVYYPVGTSMLPLIREGLDSVKLVAINDN
                     IKKYDVLLYKRNNHQFVLHRVVKVHHDSYDFMGDNQWVIEKNIKRSQIIARMEEVYRQ
                     EKTISLTTFNYQIYVRFRVWFRCLRKLKYLIKKRLFKKQDKRTK"
     gene            695..>1032
                     /locus_tag="PK087_05300"
     CDS             695..>1032
                     /locus_tag="PK087_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18720.1"
                     /translation="MKTKILFLLSLLFLVMIKCSIGKENSPQMEIKEITLSEEGLDVL
                     LQLKLEQDDKQVLKVGVLYGLVEEEELRVENALDKVELPLHDLLQPNDYLFSFHEIQE
                     SHFNKELRLQP"
BASE COUNT          362 a          130 c          204 g          336 t
ORIGIN      
        1 ttgtgtttac tttggatgat tgaagttttt gcttgtgatt gtgagtattc ggaaaaggaa
       61 gtcaatgcga agattaaagc catatatccg gactttgtga ccgttagacg cgattttgtg
      121 gattacggtt ttatgaagcg gaccgatgac ggagccaaat attggcggat aaaatgattt
      181 ttaaacaatg ctgtttaata atcattgaga aatcatgatg gctgtgttag aattattaca
      241 atactggaga gcaactcatg aatggaatga catatcaaga aataattgat aaatatggcg
      301 gctttgttta ttatcctgtc ggcacaagta tgctacctct gattcgtgaa ggattggatt
      361 cagtaaaatt agtggccatt aatgataata ttaaaaagta tgatgttctt ttatacaaac
      421 gcaataacca tcaatttgtt cttcatcgag ttgttaaggt tcatcatgat tcatatgatt
      481 ttatgggtga taatcaatgg gtaattgaaa agaacattaa aagaagtcag attattgcgc
      541 ggatggaaga agtttatcgg caagaaaaaa caatatcatt aacaactttc aattatcaaa
      601 tatatgttcg tttccgagta tggtttcgat gcttacggaa attaaagtat ttaattaaaa
      661 aaagattatt taaaaaacaa gataagagga caaaatgaaa actaaaatac tatttttatt
      721 atctttgctt ttccttgtga tgattaaatg cagtatcggc aaggaaaact ccccacaaat
      781 ggaaataaag gaaataactc tttctgaaga aggattggat gttttgttgc agcttaagct
      841 tgaacaggat gataaacaag ttttaaaagt cggggtttta tatggattgg ttgaagaaga
      901 agaattaagg gttgaaaatg ctctcgataa agtcgaatta ccattgcatg atttgcttca
      961 accaaatgat tatttatttt cttttcacga aattcaagaa tcacacttta ataaggaact
     1021 aaggcttcaa cc
//
LOCUS       DAOSGN010000416         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_157143_length_1031_cov_2.535861, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000416 DAOSGN010000000
VERSION     DAOSGN010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157143_length_1031_cov_2.535861"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK087_05305"
     CDS             complement(<1..198)
                     /locus_tag="PK087_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18721.1"
                     /translation="MAKVLTFIKDFLLPTLLSLGMMLLKIIVIITLILVTIEVLKALK
                     VLQWINKKIYVITKYLGISPSA"
     gene            complement(198..635)
                     /locus_tag="PK087_05310"
     CDS             complement(198..635)
                     /locus_tag="PK087_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOH18722.1"
                     /translation="MEQGISLSEALKNGLKQSLKVIIKIIKIMVPVYIIAQLLDYYEI
                     LAQISVYLEPLMRWFGLPGSAAIVLLLANGINIYAGIGALGALSLTSKQITILAIMVT
                     TSHSLIIETAILKTIQIPRWFHLLFRIGMMLVLGFVLNLVWRS"
     gene            complement(644..>1031)
                     /locus_tag="PK087_05315"
     CDS             complement(644..>1031)
                     /locus_tag="PK087_05315"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOH18723.1"
                     /translation="NAWDYAEALNALNLPGVVFRPASFTPTFSKHANTLCHGVQVHVI
                     DRATFQAVKTGWALVDVVRDMYPNDFTILNPNTKLNTMNLLTGGNFITKDLYTLEQQF
                     ALIIADTETFSATRNKYLLYIEEPQA"
BASE COUNT          346 a          193 c          194 g          298 t
ORIGIN      
        1 cgccgatggt gatattccta aatatttggt aataacataa atttttttat taatccactg
       61 gaggactttt aaagctttta aaacttcaat cgtcactaaa ataagggtga tgatgacgat
      121 aatcttaagc agcatcattc ctaatgataa taaggtgggg agtaagaagt ctttgataaa
      181 agttaaaacc ttagccatta cgacctccaa acgagattaa gcacaaaacc caaaaccaac
      241 atcatgccaa ttctaaacaa aagatgaaac cagcgaggaa tttgaatggt ttttaagatg
      301 gccgtctcaa taatcaaaga atgtgaggtt gtcaccataa tggctaaaat cgtgatttgt
      361 ttgcttgtta agcttaaggc ccccaaggca ccaatacccg catagatatt aatcccatta
      421 gcgagcaaaa gcacaatggc agcggatccc ggcaagccaa accaacgcat aagcggttct
      481 aaataaaccg atatttgagc taagatttca taataatcta atagttgcgc gatgatataa
      541 accggaacca tgattttaat gattttaata atgactttca gggattgttt taaaccattt
      601 ttaagagctt cactaagaga aattccttgt tccataatca tccttatgct tgaggttctt
      661 cgatataaag taaatatttg tttctagtag cactaaaagt ttctgtgtcc gcaataatta
      721 aagcaaactg ttgttctaaa gtatataaat ccttggtaat gaagttgcca cccgtcaaga
      781 gattcatcgt attcagttta gtgttcggat tgagaatggt aaaatcattc ggatacatat
      841 ctctaaccac atccactaag gcccaaccgg ttttaaccgc ttggaaagtc gctcgatcta
      901 ttacatgtac ttgcactcca tggcataggg tgttggcatg tttcgagaaa gttggtgtaa
      961 atgaagcggg ccgaaaaaca acaccgggta aattgagggc atttaaggct tccgcataat
     1021 cccaagcatt g
//
LOCUS       DAOSGN010000417         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_157219_length_1031_cov_1.821721, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000417 DAOSGN010000000
VERSION     DAOSGN010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157219_length_1031_cov_1.821721"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PK087_05320"
     CDS             <1..956
                     /locus_tag="PK087_05320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOH18724.1"
                     /translation="LIYTLIIVISLFTIIFIRFFVKKKDYPLKMLKWFYHLFWLINML
                     LVITVYLMDAYSSGKTASLFGFYLVLAAVPILSFWEIFLFLLLPNALMVGVWGFNILT
                     LKEAIYTIFIYEALALVISQATYQTFLRMLDNEIKLHQSAKKLELMSLQDPLTGLNNR
                     FGLTRAFNELKAGAKHNDLLFVIAIDIDFFKRFNDQHGHLVGDECLVHLANNIRTVFC
                     DNNSAVGRIGGDEFIILYKTTFFKEVEWKMEMMYASLDELSQLLPLEETPISLSSGIA
                     FAELKQLTTWTYLYEDADSLLYKIKNSGRNQFLFATKKEAE"
BASE COUNT          280 a          171 c          203 g          377 t
ORIGIN      
        1 atctaattta tacattgata attgttatta gtttatttac catcatcttt attcggtttt
       61 ttgtgaagaa aaaagactat cctttaaaga tgttaaaatg gttttatcac ttgttttggc
      121 tcattaatat gcttttggtt attactgttt atttaatgga tgcttacagt tccggcaaaa
      181 ccgcttcctt atttggtttt taccttgttt tggcggcagt gccgatttta tctttttggg
      241 aaattttctt gtttttgctt ttaccgaatg ctttaatggt gggtgtttgg ggctttaata
      301 ttttgacctt aaaggaagcg atttacacga tttttattta tgaagccctg gccttggtca
      361 tttcccaagc gacttatcaa accttcctaa ggatgctgga taatgaaatt aaactgcatc
      421 aatccgctaa aaaactggaa ttgatgtcct tgcaagatcc attgaccggc ttaaacaatc
      481 gttttggttt aactagagct tttaatgaat taaaagccgg cgctaaacat aatgatttat
      541 tgtttgtgat tgcgatcgat attgatttct tcaaacgctt taatgaccaa catggtcatt
      601 tagttggtga tgaatgctta gtgcatcttg ccaacaatat tcgcaccgtt ttttgtgata
      661 acaatagtgc cgtcggtcgg attggcggtg atgaattcat tattttatat aaaaccacct
      721 tctttaaaga agttgaatgg aaaatggaaa tgatgtatgc ctctttggat gagctttctc
      781 agttgttgcc gcttgaggaa acaccgattt ccttatcgag tgggattgct tttgcggaac
      841 ttaagcaatt gacaacttgg acttatcttt atgaagatgc ggattccctc ttgtataaaa
      901 ttaagaatag cggtcgcaac caatttttgt ttgcgactaa gaaagaagca gaatgaagac
      961 catctttgct taccaattcc ctattggcac gctgtggatt agcgaacaag gtggaaacat
     1021 catcgctatt g
//
LOCUS       DAOSGN010000418         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_157368_length_1030_cov_2.715897, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000418 DAOSGN010000000
VERSION     DAOSGN010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157368_length_1030_cov_2.715897"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PK087_05325"
     CDS             <1..589
                     /locus_tag="PK087_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOH18725.1"
                     /translation="NDLWLTKMTSSYWDLVHRGVEDAHRLDIPTVIFHVSSTSTPPEI
                     SDIGLKRFSELLKKCEVYRINLALENLRRLDYLDALFAKFSSPYLKFCFDSGHANAFT
                     KNIEQFPWEKYQSYLHCLHLHDNNGLRDQHLPPFLGTINWTLLISNLKRCQYQGPLTV
                     ESVKHPSLDLTEEAFLEQIKKAHVQMENLWLGNEN"
     gene            579..>1030
                     /locus_tag="PK087_05330"
     CDS             579..>1030
                     /locus_tag="PK087_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOH18726.1"
                     /translation="MKTELLAPVGSWDAFYAAIYNRADAVYLGGKAFGARAYADNFTN
                     EEIKDIIVMAHLHGVKVYVTINTLVYDHEFAQLIDFLDFLYLHDCDAVIVQDLGVAQL
                     ILHRYPKMALHASTQMNIHSLAQVQALKARGFKRVILAREVSLTEIIA"
BASE COUNT          291 a          186 c          204 g          349 t
ORIGIN      
        1 taatgatttg tggttaacaa aaatgacatc ctcgtattgg gatttagtcc atcgtggtgt
       61 tgaagatgct catcgtttag acattccgac agttattttt catgtttcca gtacttcaac
      121 cccaccggaa ataagtgata tcggcttaaa gagattttct gagttgttga aaaaatgtga
      181 agtctatcgc atcaatttag ctttggaaaa tctgcggcgc ttggattatt tagatgcttt
      241 gtttgctaaa ttttcgtcgc cttatttaaa gttttgtttt gacagtggtc atgccaatgc
      301 ttttaccaaa aacattgagc aattcccttg ggaaaaatac caaagctatt tacattgcct
      361 tcatcttcat gataataacg gtttaagaga tcaacacttg ccgccttttt taggaacgat
      421 taattggacg ctgttaatta gcaatttaaa gcgttgtcaa tatcaaggac cgttaacggt
      481 tgaaagcgtt aaacatccga gtttggattt gactgaagaa gcttttcttg aacaaatcaa
      541 aaaagcacat gtccaaatgg aaaatttgtg gttgggtaat gaaaactgaa ttattagccc
      601 cagttggcag ttgggatgct ttttacgccg ccatttacaa tcgtgcggat gccgtctatt
      661 tagggggaaa agcctttggg gcccgcgctt atgccgataa tttcaccaac gaagaaatca
      721 aagatatcat tgttatggct catcttcatg gtgttaaggt ctatgtaacc attaataccc
      781 ttgtttatga tcatgaattt gcccaactta ttgatttttt ggatttctta tatcttcatg
      841 attgtgatgc cgtcatcgtt caagatttag gcgttgcaca actaatctta caccgttatc
      901 ctaaaatggc tcttcatgct tccacgcaaa tgaatattca cagtttagct caagtgcaag
      961 ctttaaaagc taggggcttt aaacgggtta ttttagcacg tgaagtttcg cttactgaga
     1021 ttatcgctat
//
LOCUS       DAOSGN010000419         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_157792_length_1028_cov_4.471737, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000419 DAOSGN010000000
VERSION     DAOSGN010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157792_length_1028_cov_4.471737"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PK087_05335"
     CDS             complement(<1..530)
                     /locus_tag="PK087_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018580428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH18727.1"
                     /translation="MIALHHISKQYSVGKFRVQALEQINLQFRKNELVGIIGPSGCGK
                     TTLLNIIGGLDKASSGIMTVDGLDVSQYKSRDWDAYRNQRVGFVFQNYYLLPHLNVFE
                     NVELALSLLALPQKTRKTMVRAALEAVGLSNEALNMPNQLSGGQMQRVAIARAIVNEP
                     DVVLADEPTGALDSQTA"
     gene            complement(554..760)
                     /locus_tag="PK087_05340"
     CDS             complement(554..760)
                     /locus_tag="PK087_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOH18728.1"
                     /translation="MNKPINFRNGDIYEFKKTHPCGGKNWQLIRVGVDCKLACTTCKH
                     IIIIPRVELPKKIKRIVFHEEIPG"
     gene            complement(735..>1028)
                     /locus_tag="PK087_05345"
     CDS             complement(735..>1028)
                     /locus_tag="PK087_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18729.1"
                     /translation="EVDYQESIDRVIALLDEKLINLKDTFPQILEGPNVIGVIKVLRK
                     GFIIRIIIKTMPETHYSVEREVQKQVKELFEQNNIRLATEHLVIDREQTDQLS"
BASE COUNT          295 a          236 c          194 g          303 t
ORIGIN      
        1 gctgtttgcg aatccaaggc tccggttggt tcatcagcca aaacaacatc cggctcatta
       61 acaatcgccc gggcaatggc aacgcgttgc atttgtccgc ccgacaattg attaggcatg
      121 tttaaggctt cgttacttaa tcccaccgcc tccaaagctg ccctaaccat ggttttacgg
      181 gttttctgcg gtaaagccaa cagcgataag gccagttcaa cattctcgaa aacatttaaa
      241 tgggggagta aataataatt ttgaaaaaca aagccgaccc gttgattgcg ataagcatcc
      301 cagtcccggc ttttatattg ggaaacatca agcccgtcga ccgtcattat tcccgaagat
      361 gccttatcca aaccaccgat gatatttaaa agcgtggttt taccgcaacc cgacggcccg
      421 ataattccca ctaattcgtt tttacgaaat tgtaaattaa tttgttcaag ggcttgaacc
      481 cgaaatttac cgactgaata ttgcttggaa atatgatgca aagcaatcat ggcatcctcc
      541 tttcaaaaaa tccctaacca gggatttctt catgaaatac aatccgttta atttttttag
      601 gaagttcaac gcgggggata atgataatgt gtttgcaagt tgtacatgcc aacttgcaat
      661 cgaccccgac ccgaattaat tgccaattct tccccccgca agggtgggtt tttttaaatt
      721 cataaatatc gccattacga aagttgatcg gtttgttcac gatcaatcac caagtgctcc
      781 gttgccaagc gaatgttgtt ttgttcaaac aactctttca cctgcttttg cacttccctt
      841 tcgacagaat aatgcgtttc aggcatggtt ttaatgatga tgcgaataat aaagcctttt
      901 cttaagacct taataacgcc gataacattg ggaccttcta gaatttgcgg gaaagtatct
      961 tttaagttaa tcagtttttc atcaagaagg gcgataacgc gatcaatact ttcttgataa
     1021 tcaacttc
//
LOCUS       DAOSGN010000420         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_158534_length_1025_cov_2.928866, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000420 DAOSGN010000000
VERSION     DAOSGN010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158534_length_1025_cov_2.928866"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /locus_tag="PK087_05350"
     CDS             complement(<1..91)
                     /locus_tag="PK087_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH18730.1"
                     /translation="MKVTIREIAKKANVSVATVSRAINNKGYVH"
     gene            complement(160..>1025)
                     /locus_tag="PK087_05355"
     CDS             complement(160..>1025)
                     /locus_tag="PK087_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH18731.1"
                     /translation="TFATTPMMPKWNVSHPLVEAYLLKVGTYWVQEYQIDAWRLDVSD
                     EVSHEFWRKFKKTIQAINPEVYIVGENWDNANPWLLGDQFDAVMNYRLAYSLWNFLET
                     PAKIDALEFTYAINTLLFKYPKHVAPHMFNLVDSHDTPRILTRLHNNLGLMKIAYLFI
                     FTFTGSPALFYGDEIGLQGHHDPDCRRCMIWDDSKHNHELLAFFKWLINLRKRALDWQ
                     AVTIKWLYAQAKAFAYEKGAYVFVINADQHEHHSPLKGPFIDLVSQNLIKDELPTLLP
                     YSFLALQKTDC"
BASE COUNT          307 a          204 c          212 g          302 t
ORIGIN      
        1 catggacata acctttattg ttaattgccc gactgactgt agccacgctg acattggcct
       61 tcttagcaat ttctctaatc gttactttca tattaaagac ctctttagta aataaattat
      121 atcaaacaat tactttgaaa gcaaattaga aaaccacttt caacaatcgg ttttttgtaa
      181 agcgaggaaa ctgtaaggaa gaagggttgg cagttcgtcc ttgatgaggt tttggctcac
      241 taaatcgata aacggacctt ttaaagggct atggtgctcg tgttggtcgg cattaatcac
      301 aaaaacatag gctccttttt cataagcaaa cgccttagct tgtgcatata gccacttgat
      361 ggtgactgct tgccaatcta gtgccctctt tcgtaaatta atcagccatt taaaaaaggc
      421 taataattcg tggttgtgct tactgtcatc ccaaatcatg cagcgacgac agtcggggtc
      481 gtggtggcct tgaaggccga tttcatcacc ataaaaaagg gcggggctac cggtaaatgt
      541 gaaaataaac aaataagcaa ttttcattag accgaggtta ttgtgcaaac gggttaaaat
      601 tcttggggta tcatgggaat caactaaatt aaacatatgt ggagcgacat gtttaggata
      661 tttaaataac agcgtattga tggcataagt aaattctaag gcatcaattt tggctggggt
      721 ctccaagaaa ttccataaac tataagctaa gcgatagttc atgacggcat caaattggtc
      781 gcctagaagc caaggattgg cattgtccca attttcgccg acaatgtaga cttcagggtt
      841 aatcgcttgg attgtttttt tgaatttgcg ccaaaattca tggctgactt catcggaaac
      901 atccaaacgc caagcgtcaa tttggtattc ttgcacccag taagttccca cttttaataa
      961 ataggcttcc acaagcggat gactgacatt ccatttcggc atcatcggcg tcgtcgcaaa
     1021 agtcc
//
LOCUS       DAOSGN010000421         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_159067_length_1023_cov_2.526860, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000421 DAOSGN010000000
VERSION     DAOSGN010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159067_length_1023_cov_2.526860"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PK087_05360"
     CDS             complement(<1..263)
                     /locus_tag="PK087_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOH18732.1"
                     /translation="MKKCLIALIVVIGIFMVMIIIAKSFSRQFNNLEIAAIDLKTLND
                     GEYIGYYKIFPISAKVKVLVANHEIQNIEILEHNNGQGKPAEA"
     gene            complement(296..>1023)
                     /gene="rlmD"
                     /locus_tag="PK087_05365"
     CDS             complement(296..>1023)
                     /gene="rlmD"
                     /locus_tag="PK087_05365"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOH18733.1"
                     /translation="FMLVLVTNTKELPHANQLIKSLQNEFPLIKTIIQNINNEKTNVV
                     LGKKNLVHMGSGFLREELLGLTFAVSPHSFFQTNYEQTSKLYRQVLKYANPQLTDVIV
                     DGYCGVGTIAVFLARFCKRVVGIEIVKEAVQDARANALLNNISNVEFIVGKTEEEIYK
                     FSLGEINILIIDPPRKGCAPQLLQAIIEKQIPKIVYVSCNVATLARDLAFLSSNYEIK
                     DVTFFDMFPQISDVEICVLLQIT"
BASE COUNT          349 a          194 c          143 g          337 t
ORIGIN      
        1 atggcttccg ctggttttcc ttgaccgttg ttgtgttcta atatctcaat attttgaatt
       61 tcgtgattgg caacaaggac ttttactttg gcgctaatgg gaaaaatctt ataataacca
      121 atatattctc catcattaag cgtctttaaa tcaatggcag caatttccaa attattaaat
      181 tgccgggaaa atgacttcgc aataataatc atgaccataa aaatcccaat aacaacaatt
      241 aaagcaatta agcatttttt catatattta ctcctaaaaa acataaactt aataattatg
      301 ttatttgcaa taaaacacaa atttccacat cgctaatttg cgggaacata tcaaaaaaag
      361 ttacatcttt aatctcataa ttacttgata agaatgccaa atcacgagct aaagtagcaa
      421 cattacagga aacatatact attttcggaa tttgtttttc gataatggct tgtaataatt
      481 gcggagcaca ccctttccgt ggtgggtcaa taatcaaaat attaatttct cctaacgaaa
      541 acttgtagat ttcctcttcc gttttcccga caataaactc tacattacta atgttgttta
      601 gtaaagcatt ggctctagca tcttgaacag cttccttcac aatctctata ccaacaaccc
      661 gcttacaaaa cctagctaaa aaaacggcaa ttgttcccac tccacaataa ccatcaacaa
      721 ttacatcagt aagctgggga ttagcatatt ttaaaacttg gcgatacaat ttacttgttt
      781 gctcataatt ggtttggaaa aacgaatggg gcgaaacagc aaaagttaaa cccagcaatt
      841 cttcacgtaa aaagccgctt cccatgtgga caagattctt tttccctaaa accacattgg
      901 ttttttcatt gttaatattt tgaattatcg ttttaattag cggaaattca ttttgaagcg
      961 atttaattaa ttgattagcg tgaggcaatt ctttagtatt ggttactaat accagcataa
     1021 att
//
LOCUS       DAOSGN010000422         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_161753_length_1012_cov_3.565308, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000422 DAOSGN010000000
VERSION     DAOSGN010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161753_length_1012_cov_3.565308"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..701
                     /locus_tag="PK087_05370"
     CDS             138..701
                     /locus_tag="PK087_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HOH18734.1"
                     /translation="MQIIFGGSFNPPTIAHIKIAQYLLDNFPEAELIFLPTNNFHIKD
                     NLQDLRFRWQMLEIVCRFLNNRPTISDFELKLDRFYGTDYTLKHFPKAHFVIGADNLL
                     TLPSWLNFPNVVINHQFIVFPRHQLDMENIILQHPILKQYRHHFMLINDFSEMYISSS
                     DFRKTLDERLVIPEIAHFIKENHLYKE"
     gene            705..>1012
                     /locus_tag="PK087_05375"
     CDS             705..>1012
                     /locus_tag="PK087_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOH18735.1"
                     /translation="MYSQGFLKVAAACPTARVGDVMFNVKQMLTMLQEATSKDPAIIC
                     FPELCITGYSVGDLVFQKYLYDDSLKAINYLLEHNPFAGVIIFGSFIIINDTIYNCGF
                     V"
BASE COUNT          301 a          179 c          157 g          375 t
ORIGIN      
        1 ccctaacccc ctttgttctt taaatctttt ttctattttc attttacaat attcagtgtt
       61 ttttgtttgc acgaatttac ttaacaaaaa aaggctttta tgataaaata actatgacaa
      121 aacaaagagg aataaacatg caaattattt tcggtgggtc ctttaatcca cctacgattg
      181 ctcacatcaa aatagctcaa tatttgttag ataactttcc cgaagcggaa ttgatttttt
      241 taccaacaaa taattttcac atcaaggata atcttcaaga ccttcgtttt cgttggcaaa
      301 tgctggaaat tgtttgccgt tttctaaata atcgtccgac aatcagtgat tttgaattaa
      361 aattggatcg cttttatgga actgattata ctttgaagca ttttcctaag gcccattttg
      421 ttattggagc tgacaattta ttaacgcttc ccagttggct taattttcct aatgttgtca
      481 ttaatcatca atttattgtt tttccgcgcc atcaactcga tatggaaaat atcattcttc
      541 aacatccgat tttgaagcaa tatcgacatc attttatgct catcaatgat ttttccgaga
      601 tgtatatttc atcaagtgat tttcgaaaaa cacttgatga gcgcttagtg attcctgaaa
      661 tcgctcattt tattaaagaa aaccatttat ataaggagta aaaaatgtat tcacaaggat
      721 ttttaaaggt ggctgccgct tgtccaactg ctcgtgttgg tgatgtgatg ttcaatgtta
      781 aacaaatgtt aaccatgctg caggaagcga catccaaaga tcccgccatt atttgttttc
      841 cggaactatg tattactgga tattcagtgg gcgacttggt ttttcaaaaa tatttatatg
      901 atgattccct taaagccatt aattatttac tagagcataa tccctttgcc ggagtaatta
      961 tttttgggtc cttcattatc attaacgata ccatttataa ttgcggtttt gt
//
LOCUS       DAOSGN010000423         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_161828_length_1012_cov_2.333333, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000423 DAOSGN010000000
VERSION     DAOSGN010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161828_length_1012_cov_2.333333"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PK087_05380"
     CDS             complement(<1..462)
                     /locus_tag="PK087_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH18736.1"
                     /translation="MKMIKWLKKLFNKKQKKSSKKGGTNLDVKEIVKLILEGKQPNEE
                     QLQALKDFVGKDEQEQPSTEDNPGETNPDETKPDEETKPSETEEVSNEETPQEEATEK
                     KDNPDGEETEDGKEGGEAEETGQPPAQVQTEPTNTPPAVNQTLMNKCANFVI"
     gene            complement(473..763)
                     /locus_tag="PK087_05385"
     CDS             complement(473..763)
                     /locus_tag="PK087_05385"
                     /inference="COORDINATES: protein motif:HMM:NF022642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HOH18737.1"
                     /translation="MTFSEALEAVKNGKAITRKGWNGKNQFVFMTPGRTLELGKHDIW
                     TKHVQEVARQNGGKVVIQPYLNIKTVKNEIQIGWLATQSDMLANDWEIVETF"
BASE COUNT          204 a          224 c          177 g          407 t
ORIGIN      
        1 aattacgaag ttcgcgcatt tgttcatcaa agtttggttg actgcgggtg gggtattggt
       61 aggttcagtt tgcacttgag ccggtggttg ccctgtttct tcagcttctc caccttcttt
      121 tccatcttca gtttcttcgc cgtccggatt atctttcttt tccgttgctt cttcttgagg
      181 tgtctcttcg ttgcttactt cttctgtttc gcttggcttg gtttcttcat ccggctttgt
      241 ttcatcgggg tttgtttctc ctggattatc ttcggtcgat ggttgctctt gttcatcctt
      301 accaacaaaa tcttttaaag cttgcaactg ctcttcattt ggttgtttac cttccaaaat
      361 tagctttaca atttctttta catccaaatt ggtgcctcct ttctttgatg attttttttg
      421 ttttttatta aatagtttct tcaaccactt aatcattttc attcctccag tcttaaaatg
      481 tctcaacaat ttcccagtca ttagctaaca tgtcgctttg agttgctagc cacccgattt
      541 ggatttcgtt ttttaccgtt ttaatattca agtaaggttg aattacaacc ttaccgccgt
      601 tttgtcttgc aacctcttgg acatgtttag tccaaatgtc atgcttcccc agttctaatg
      661 tgcgtcctgg cgtcataaag acaaattgat ttttgccgtt ccatcctttt cgtgtaatgg
      721 ctttcccgtt ttttactgct tctaaagctt cgctaaatgt cattttttca cctccagtct
      781 gtataaaaaa agacccatta aataggtctt gtgaatacat ttattgcatt gtgagcgata
      841 tttaaaccaa ttttaggcat aagaaaagca cctttaacag gtgctttatg acttaattat
      901 gttgttaatt atttaacagg tcgtccctca cgccaagcct tacgggcttc gttaaggctc
      961 atttcgtttg gtccaccgct tggttcatcg gggttatcat gggcaacttc at
//
LOCUS       DAOSGN010000424         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_161875_length_1012_cov_1.894462, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000424 DAOSGN010000000
VERSION     DAOSGN010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161875_length_1012_cov_1.894462"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PK087_05390"
     CDS             <1..797
                     /locus_tag="PK087_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH18738.1"
                     /translation="EKNHASIIDGTRLSKAERTIYRHNDMDDLERILKEKRSQYRNVL
                     IITDGVFSMDGDLAKLPNIVALAKKYQALTYVDDAHGSGVLGRSGRGTVDHFHLHGQV
                     DFIIGTLSKAIGVVGGYVASKQIVKDWLLHRARTLLFSTALPPAAIGATIESVKMLME
                     SETYTQKLWENARYFKKALGNLGFDLGHSETPITPVMVGKESLTMELSRGLLAEGVFV
                     SGIVFPTVPAGQGRLRVMLSASHTKANLDFAIAAFAKVGKKLRIIN"
     gene            797..>1012
                     /locus_tag="PK087_05395"
     CDS             797..>1012
                     /locus_tag="PK087_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOH18739.1"
                     /translation="MKAFPKQYIVAHRGASGLVPHENTLEAFQKAIDVGADCIELDVR
                     KTKDQQIIVIHDDHLLEAVVKETNYADL"
BASE COUNT          284 a          216 c          238 g          274 t
ORIGIN      
        1 atgaaaaaaa ccatgcctcg attattgacg gcacccgttt atcaaaagcg gaacggacaa
       61 tctaccgcca caacgacatg gatgatttag agcgaatctt aaaagaaaaa cgttctcaat
      121 accgtaatgt gttaattatc actgacggtg tcttttccat ggatggcgat ttagccaaat
      181 tgcctaacat tgttgctctg gccaaaaaat accaagcttt aacttatgtt gatgatgccc
      241 acggtagtgg ggttttagga agaagtggcc gtggaaccgt cgaccatttc catttacatg
      301 gtcaagtaga tttcattatc ggaactctca gcaaagccat cggtgttgtc ggtgggtatg
      361 tcgcatccaa acagattgtg aaggattggt tattacatcg tgctcgcacc ttgctttttt
      421 ccactgccct accaccggcg gccatcggtg ccaccattga atctgttaag atgctgatgg
      481 aaagcgaaac ctacactcaa aaactatggg aaaatgcccg ttactttaaa aaggctctcg
      541 gcaatctcgg ctttgatcta gggcacagtg aaaccccgat tacccccgtt atggtgggaa
      601 aagaaagcct aacgatggaa ttatcgcgcg gtttattggc ggaaggcgtc tttgtttctg
      661 ggattgtttt tccgacagtt cccgctggac aaggacggtt acgcgtgatg ctgtcagcta
      721 gtcataccaa ggctaatttg gatttcgcca ttgccgcttt tgcgaaggtg gggaaaaaat
      781 tgaggattat caattaatga aagctttccc taaacaatat attgtggcac accgcggtgc
      841 ctcgggttta gtaccacatg aaaatacctt agaagccttc caaaaagcga ttgatgtcgg
      901 ggccgattgc attgaacttg atgtgcgaaa aacgaaggat cagcagatta tcgtcatcca
      961 tgatgaccat cttttggaag cagtggtaaa agagaccaat tatgctgatt ta
//
LOCUS       DAOSGN010000425         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_162344_length_1010_cov_2.181152, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000425 DAOSGN010000000
VERSION     DAOSGN010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162344_length_1010_cov_2.181152"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1010)
                     /gene="polC"
                     /locus_tag="PK087_05400"
     CDS             complement(<1..>1010)
                     /gene="polC"
                     /locus_tag="PK087_05400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOH18740.1"
                     /translation="FYKEPRILKTYLEAHREGLLLGSSCCNGEIFDLAWRESYETLLS
                     ALHFYDYIEIQPVDHYLHLFEDDDIEANKDLIKQILKKIIQASQECGKPVVATGDVHI
                     LNSDDYKLREIFINAPQVGGGIHKLFNKNNIATQHFMTTEEMLQAFSFLGDDLAKQIV
                     VTNSHLIADQIEYHPLFDDYLYTPSDDFMKDLGVPSFKQATIDLTYQSAHRRYGQNLP
                     LYILERIKKELNSIINNNFASIYYISHLLVKKSQDDGYVVGSRGSVGSSLVAFFMGIT
                     EVNSLPPHYYCSHCHFVALKLNNEEKKKYSQPNVTDIIYSLLQTAGTGFDLPDQKCPV
                     CG"
BASE COUNT          335 a          189 c          172 g          314 t
ORIGIN      
        1 tcccacaaac aggacatttt tggtcaggta aatcaaagcc cgtgcctgcg gtttgtaaga
       61 gagaataaat gatgtccgtc acattgggtt gtgagtactt ctttttttct tcattgttca
      121 acttaagagc tacaaaatga cagtgcgaac agtaataatg gggtggcaag gaattaactt
      181 ccgtaattcc cataaaaaag gcaactaaac tggagcccac cgaaccacgg ctaccaacaa
      241 cataaccatc atcctgtgat ttcttcacaa gcaagtggga tatgtaataa atggaagcaa
      301 aattattatt aataatgctg ttgagttctt tttttatccg ttccaatata tataatggta
      361 agttttgacc ataacgacga tgagcgcttt gataagtcaa atcaattgtt gcctgtttaa
      421 atgatggcac tcctaaatcc ttcataaagt catcgctggg ggtatataaa taatcgtcaa
      481 aaaggggatg atattcaatt tgatcagcaa ttaaatggga attcgtcacc actatttgtt
      541 tagctaagtc gtcacctaaa aaagaaaacg cctggagcat ttcttccgtc gtcataaaat
      601 gctgagtagc aatgttattt ttattaaata atttgtgaat tcctccccca acttgcgggg
      661 cattgataaa gatttcccgt aatttataat catcactatt taaaatgtga acatcaccgg
      721 tagcaaccac tggtttgcca cattcttgac tggcttgaat gattttttta agaatttgtt
      781 tgattaaatc tttattagct tcaatatcat catcttcaaa taagtgtaaa taatgatcaa
      841 ccggttgaat ttcaatataa tcataaaaat gaagtgctga taataatgtc tcataactct
      901 cacgccatgc aagatcaaag atttcgccat tacaacaact actccctaaa agcaaaccct
      961 cacgatgagc ttctaaatat gttttgagga tacgaggctc cttataaaaa
//
LOCUS       DAOSGN010000426         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1752
            NODE_164460_length_1002_cov_1.810982, whole genome shotgun
            sequence.
ACCESSION   DAOSGN010000426 DAOSGN010000000
VERSION     DAOSGN010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863281
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:11:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,082
            CDSs (total)                      :: 1,078
            Genes (coding)                    :: 1,063
            CDSs (with protein)               :: 1,063
            Genes (RNA)                       :: 4
            tRNAs                             :: 4
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164460_length_1002_cov_1.810982"
                     /isolate="AMR_MDS_1752"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..595)
                     /locus_tag="PK087_05405"
     CDS             complement(38..595)
                     /locus_tag="PK087_05405"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOH18741.1"
                     /translation="MKKEETVSQNYLYQGKVLNLRIDDVRLPNGDLGHREIVEHHGGV
                     CGLAVNKEGKILFVKQYRIALDDFILELPAGKIEIGENPEATIVRELEEEIGIKVNQI
                     EKAGIIYPSPGYTSEVIHLFFVRDFELTVNHLDEDEFLEIVPIDINTAFEMLDKNEIT
                     DAKSLVLLSKYRDELIKFSKLTKRQ"
     gene            complement(600..>1002)
                     /locus_tag="PK087_05410"
     CDS             complement(600..>1002)
                     /locus_tag="PK087_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein 8"
                     /protein_id="HOH18742.1"
                     /translation="GKTKTLNFFLINKKFYLVDAPGYGYVKHGREKIATFGSLIDQYL
                     LGNRNLKLAFLLVDTKVGPTGDDVLMLNYLRYLKIDYIVVATKSDQIGSTLLYRHLKR
                     IYEKLAITEKELIVTSSKEKMGFEKIHDLLK"
BASE COUNT          317 a          199 c          144 g          342 t
ORIGIN      
        1 ttctctttaa tgttgtttat ggctgtgatt tgctaaatca ctgtcttttc gttaatttac
       61 taaatttaat taactcatct cgatattttg ataacaatac caatgatttg gcatccgtaa
      121 tctcgttttt atcaagcatt tcaaaagcag tattaatatc aatcgggaca atttccaaaa
      181 actcatcttc gtctaagtgg ttgactgtta attcaaaatc acgaacaaaa aacaaatgaa
      241 taacctcact agtatatccc ggtgagggat aaataattcc cgctttttca atctggttga
      301 ctttaatacc gatttcttct tccaattccc gaacaatcgt ggcttcaggg ttttcaccaa
      361 tttctatttt tccggccggc agctcaagaa taaagtcatc aagagcgatg cgatattgtt
      421 tcacaaacaa gattttacct tccttgttga cggctaaacc gcaaacccca ccgtgatgtt
      481 caactatctc ccgatgacct aagtcaccat ttggtaaacg tacatcatca atccgtaaat
      541 ttaggacttt accttgatat agataatttt gtgatactgt ttcttctttt ttcataagcc
      601 tatttcaaca aatcatggat tttttcaaat cccattttct ccttagatga agtaacaatg
      661 agctcttttt cggtaatcgc taatttttca taaattcttt ttaaatggcg atataagagg
      721 gttgacccaa tttggtccga ttttgtagca acaacaatgt aatcaatttt taaataccgt
      781 aaataattaa gcatcaaaac atcatcaccc gtgggaccga ctttagtgtc aacaagtaaa
      841 aaagctagct ttaaattgcg attgccaagc aaatattgat caataagtga tccgaaagtc
      901 gcaatttttt ctcgtccgtg cttaacatag ccataaccgg gagcatcaac caaataaaac
      961 tttttattaa ttaaaaagaa attaagcgtt ttagttttac ca
//
