LOCUS       DAOSGP010000001        16052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_2986_length_16052_cov_19.354066, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000001 DAOSGP010000000
VERSION     DAOSGP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 16052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16052
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2986_length_16052_cov_19.354066"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..282)
                     /locus_tag="PLV49_00005"
     CDS             complement(118..282)
                     /locus_tag="PLV49_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97252.1"
                     /translation="MAGVSEKAVIQDANEAALRKFEEGVRRSIRQADKGLVKTFKTKE
                     EFLNHLKNLE"
     gene            complement(342..1457)
                     /locus_tag="PLV49_00010"
     CDS             complement(342..1457)
                     /locus_tag="PLV49_00010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG97253.1"
                     /translation="MEIAYVYDAVYPWVKGGAEKRIYELSRRLAARGHVVHCYGMKWW
                     QGEDEIEREGVRFHGICEPRPLYVNGKRSIGEAAYFAARLLAHPSQEEVVDCQNFPYL
                     SCFSARLLSSWRKKNQKLFITWHEVWKDYWHEYLGRKGEMGRITELIAARLTDRNLAV
                     SKRTARDLEGLGARGVQVVPNGIDWQKIEGIAPSPKSSDIVFAGRLAAHKNIHLLIQA
                     VSIMKSELPDVRAAIIGDGPEMERLRSLTEKLGLEKNIEFCGFLENYDDALALMKSSR
                     AFASPSTREGFGMAALDANACGLPVVTVEHRMNAVMDLVTENTGIICPPAAQDLAEGL
                     LLALQESPRMREPCLKLAQGYDWERICDLVEKIYEVE"
     gene            complement(1461..2585)
                     /locus_tag="PLV49_00015"
     CDS             complement(1461..2585)
                     /locus_tag="PLV49_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG97254.1"
                     /translation="MPRISLIIPTRNESQTIRQCIHRALEAFEKAGLEGEIIVADSST
                     DQTADIAASCGAKVVFPQRLGYGNAYLLGFQEAGGDYIFLMDGDLTYDPETILDMLPC
                     LESGEYDLVMGSRLKGRIIPGAMPALHRYVGNPILTWILNRLFSAGISDAHCGLRGIT
                     RDALYKLNLRSGGMEFASEMVIEAASRGLRIAEVPITYYPRQGDSKLNSFTDGWRHMR
                     FMMLYRPGPFLLVPGLVALLLGLILAVVVYIEGGSRMHSLILGGLLLIIGYQMLLAGM
                     HFRAFGAAWGLSKSSGMKKLISYHSLEKELLIGLALLAAGIVIGLVVLMRWRGAGFGA
                     LDAAQNAMVAMILTILGIQTIFSGMFISLLLLYNGHQDGE"
     gene            complement(2664..2900)
                     /locus_tag="PLV49_00020"
     CDS             complement(2664..2900)
                     /locus_tag="PLV49_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOG97255.1"
                     /translation="MEAPIGKSVYNFIDLVGTSNESWEDAVQSAINTANRKLTNVRIA
                     EVTRMDTRLREGGIMEYRVRVTLSFKHEVEPETL"
     gene            complement(2962..3591)
                     /locus_tag="PLV49_00025"
     CDS             complement(2962..3591)
                     /locus_tag="PLV49_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG97256.1"
                     /translation="MEEEKKMVENKPEENKTEEKAEPVITQSMREEACPTCGATEPSM
                     PAVLPDMSWSIEKLIAATKDKHMLVRSNAIMLLSKRDISESLEALIEALKDEEYLVKS
                     NAMVAIAGYGKQVSDRMIEALEDADKDVRAGAAWVIGELKENRAVAALEKVAKDDYPL
                     ARIQAKASLIAMGRGPKKPEAQPPVAQTSEDSTEEGDKEAEKSEEKKAE"
     gene            complement(3609..4196)
                     /locus_tag="PLV49_00030"
     CDS             complement(3609..4196)
                     /locus_tag="PLV49_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOG97257.1"
                     /translation="MRTSECDMVIVGVGGQGVILISDVIGRAGVLAGKPVRGAETHGM
                     AQRGGSVINHTRIGCRFSPLVAPGSADLLIALEPAEALRFGHFLSPEGVALINNQPVL
                     PVTVTTGRARYPSLDDLIEPLCGQCQRVLALDATALARKAGTAQAMNVVMLGALSQYI
                     PLSEEHIIQALVEVVPPKHLEANKRAFALGKAEVE"
     gene            complement(4193..5977)
                     /gene="iorA"
                     /locus_tag="PLV49_00035"
     CDS             complement(4193..5977)
                     /gene="iorA"
                     /locus_tag="PLV49_00035"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOG97258.1"
                     /translation="MREYLLGNAAIAKGLLEAGIGLAAGYPGTPSSEIIEALVELKGD
                     LPLHIEWSVNEKVALEVAAGASWTGCRSVATMKHVGLNVAADPFMTLAYLGVGAGLLV
                     ICADDPFCHSSQNEQDSRRFAQFASIPCLDPADPQEARDMAIYGLELSEKFNIPVMLR
                     PTTRVSHARADVEVSQDRPIPVERRFVKNPARRVALPSHARPLHHELLAKQPAIEEEL
                     EGCPWNRLTLRGETGVIASGIAGLYAEEALMDLDLDHTLLRIGAYPAPKKLICRMLEH
                     VSRVLVIEELEPVVEEQVAMLAGKINPSIQILGKEKEIPREGELDLITVRNALARMNG
                     LGNMERASFAAAEIVPPRPPALCPGCSHRATYYAMRRVYGKKAIYPSDIGCYTMAVGM
                     GTVDTCLCMGASITLASGIRFGGETEGICASLGDSTFLHGGMTGLLNAAYNKARITVT
                     ILDNSTTAMTGHQHHPGTGVTATGEPTAQVSLEGLARALGAGLVETVDPYDLEGTMEG
                     FQRAKDFPGLSVVIARQPCVIKARRSGTRPRAFQVSDECQGCRICVEYGCPAIEFEGE
                     MAKINNLCTGCGICAQICPSSAILEVER"
     gene            6126..7172
                     /locus_tag="PLV49_00040"
     CDS             6126..7172
                     /locus_tag="PLV49_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG97259.1"
                     /translation="MLQWQSDSVGSIYNYLSPGCQICRQGAGLVLFVTGRCERGCFYC
                     PISEDRRGKDVAYADEQPVGELADILSEGRAIGALGTGITGGEPLLRLDYVLDCIRAL
                     KEEFGSEHHIHLYTGILPNRSVLERLVRAGLDEIRFHPPDKEWSDPVGLKEVLEEAKA
                     LGLQAGVEIPAYKPAPQIVHAVREADAFLNLNELEFSETNFSRLMEEGFLPLDLGCGA
                     EGSEEMARGYLLDDIKVHYCPSRFKDAVQLRERLRRRAERTSRPLDYITEEGTIIHGI
                     IEGKEGDLESALGIIDGLEVPAEMYSCLEGRIEIAAWILEEICPDLEGCKCDLCIIER
                     YPLQDGPVVERIPL"
     gene            7211..7513
                     /locus_tag="PLV49_00045"
     CDS             7211..7513
                     /locus_tag="PLV49_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HOG97260.1"
                     /translation="MESAEERIQERLRNYLKRDGVGIRKAVLKLFLSDGAYTTEDVFT
                     YLAKEGFDVSYRGVSAMVGLMNTRLGILSIDVSGDHNVYSLKGDHKGVVRSVLENY"
     gene            complement(7679..9181)
                     /locus_tag="PLV49_00050"
     CDS             complement(7679..9181)
                     /locus_tag="PLV49_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG97261.1"
                     /translation="MAQFPGKRIVLTSDTTMMSSYHGGVMLGFAAIMPRATLPDWIFQ
                     NFFCPPVPALEDGSAATAPCGMRKVEAALLESGFSRDEVMVAHPLHLDRAIGPETRIV
                     GITHDDPMGKIALREIEDIIRRGPPFNRSRFIELLSHPLIKEHRPRVVLGGNGAWEVM
                     GEKVDVDHVYLGEGESDFPEICRQIIRGESVPEVLRGSAVPGENIPVNRGATIGGIVE
                     IGRGCWRGCAFCSPTMRSVRHRPIENILKDVRVNLECGQEDIILHSEDVFSYGSKGTR
                     PNEKKMTELVAEVKKLGPKTMDFSHLSLASVHSNQGLLREISKIVGIGSDQNHMSAWI
                     GIETGSCRILKMHMPQKALPGETENWPQIVGDCYRLFDEESWLPVASLVLGLPGETAD
                     DVIRTTELVESLKDYTGLMLPLFFTTIAETKLGGTRGFSKADALPEHWHLVGLCLEYN
                     LKHLKRLHSLYKERMTAGPLVHAALTGVNLIADVALNKYLKRMKRGEPPN"
     gene            9271..10773
                     /locus_tag="PLV49_00055"
     CDS             9271..10773
                     /locus_tag="PLV49_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOG97262.1"
                     /translation="MQEKSLSEINERIRNGSARVVTAEEMPDIVDELGTAGAVREVDV
                     VTTGTFGAMCSSGVFLNLGHSDPPIKIARAYLNRVEAYGGIAAVDLFLGATQPSEDRG
                     IEYGGAHVMEDLVIGKQIEVEAHGSPSDCYPQMDLETSLRLEDFNQATMVNPRNAYQR
                     YNAATNSSDRTMHTYMGTLLPGLGNVHYSGAGVLSPLSNDPGFEYIGVGTRIFLAGAQ
                     GYVIGSGTQHSPENGFSTLMVTGDLKRMNNSFLRAATFHKYGPSLYLGIGIPIPMLNE
                     KLAKSTAVQDSDITVPVQDYGIQRRDKPSVRMVSYEDLKSGTIDLGGKEVSCSPLSSF
                     QMAREVARTLKEQVERGEFLLSQAAEPLSRVGSCRPMKQTKESPNVGDVMSRDVVTVF
                     EDIPVEKAAKLIISGSFDHLPVVSRDGRLIGIITAWDISKAVASGKPSRIAEIMTRRV
                     HSVRLDEPIELAARTLDTHSISALPVVDREHKVIGMITSNHLSRLLARRR"
     gene            10785..11162
                     /locus_tag="PLV49_00060"
     CDS             10785..11162
                     /locus_tag="PLV49_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG97263.1"
                     /translation="MLHVAPGIVRHPLIASVILETEALVNIERASINAVSGEIVLEVP
                     PEKCQEVMEAFERRGVRVVPLEIPVIRDESECVNCGACVSICPTGTFRFEDWRVVADS
                     GKCIQCGACVVGCPQRALKLVLK"
     assembly_gap    11477..11576
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11577..15395
                     /locus_tag="PLV49_00065"
     CDS             <11577..15395
                     /locus_tag="PLV49_00065"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG97264.1"
                     /translation="FAEMLSDPEGYDPERLKSLSVGVPSLLSSFIEAMLSSDEDVMKR
                     SLTDLEDYASVVNSVQAWFNLGRLAHHLFFHQAAIEYYSNASDLAQILSDDDSLASIL
                     LSLGALYGEEEDWARASQCYEKALVAIDMVKRPRLLPQVLERLGRARRQQGEYHQAQI
                     CYSRILEVLDPRDKKGRMEATSNLAELCQIQGDMEKAEKLYQTILKERELSDDPVGAA
                     RALASLANIYSQTGNGKRAQISLQSASHLLADLKDQSGAAEMQCRLADIYFHEGRLKE
                     ALELYGQSLAALQKGHPSLASKALCRMGQCFLEDGNWILAEDHLQRAISIMKEHKDSS
                     SLARALVILAGVHHKQGELEMALQCSRESLKIREGSKDQSGSAEALNCMARICCDCSD
                     WPEAIECYQRAADLFMEIGEVTAASEALSNLGGVYHLKGDLDVALQIYSHAYDSFCQQ
                     GDRQGASMVQSNIGLVYQSRGDLHLAGEFLEKSIATKEDLGDQIGAAGNRLSLGLIYQ
                     LGGEWDQAQSCFEWAARAFEDNGDLRGLAAAENNLGNLYSDRGDLMHAILCYRQGLEA
                     RRVLNDGHGMAVTLGNLAGVYLQSGEEERAEELYQQSIDGFKELGDARGEARSLYGLA
                     RSMAERGDLEGALECYRKCLDLQIIGGDSRSMIEALESMVNLLLRRGRWQEAQTRYAQ
                     VLEALRSRDDLSAVAAVQCALGNLHADLGQWDEALEDYNESLAIMEGLGDEYSVSVAR
                     NNLGNMCYRQGDWAAALENYRLSLEGFGKAGDMESEASVLSNVAGLYYKRGQWADALD
                     SYSKGLSLFEKLEDSAGIAQVLGNMGNLYHRQGERDLAAEHFQRALEIYRRLKDQAGA
                     AEMQASIDMIRAGRGEISPDIEEYKRQLEELEETGDLSGQGEVLSALAGSHTDSGQWA
                     EAIACYKKSLERFDRTGEVYNRGQVLFNMALVYKDMGDLIRARTLFDEAMSIFQDLDA
                     QPCIASAKLNLGIISGQMGRSDEAIELFSQSIGILEGLGALPDLCEGYIAAALFNIQE
                     GHSMQSRFYLSQAETQVSRTDYPPLKIQLYRAQGESFLKDMLYPEAKSCFEKALALSR
                     KLSLSYEEAKAISSLGRLALASGDYEDALRRMKQALVIFGRLGASLDLIEVYRDMTLL
                     FLAQDDYQRAEEMAMLRQRQARILGHADLSLLATVDLAECEARTGKKEEARADYAGAL
                     RLAGKEGDRIPSSTIRQISEKAIEFLEAEASQPEDDRQASLLRERLGEEDYEKLLEEL
                     PHKLECRRMG"
     gene            complement(15392..16042)
                     /locus_tag="PLV49_00070"
     CDS             complement(15392..16042)
                     /locus_tag="PLV49_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97265.1"
                     /translation="METMQDLLGKVQEFASHSFTKEEARNIFGWDVSGLLVSGSKSDE
                     SSIRITFENGYNLLIKYFLNLDPTETDDTCEFVFSLKTDLTSRIKYGIHYGNYIHGQG
                     YVRLRIADTKNRMLQVMLAEFYAPAIKNVYKPIILQFKGFYDKDFFGVEVNGSGAEIY
                     YAPVRSRSEHKGARLGDVIGRLAELDLLLKESNIRQDLAKVDLQLSLLPSMMGSGL"
BASE COUNT         3403 a         4407 c         4469 g         3673 t
ORIGIN      
        1 gattatcaat aatctggctg attttcttaa gaaagcgttc ttccaatgcc ttattttttc
       61 tggtgagctt tgaaaactgc tcctcaaact caggagttga ctcaaagtcc atatggtcta
      121 ctcaaggttt ttcaagtggt tcaagaactc ttctttggtc ttgaaagtct taactaaacc
      181 tttatctgct tgcctgatac tccttcttac accctcttca aacttcctta gggcggcctc
      241 attggcatcc tggattacgg ctttctcgct tactccggcc atagtaggta ttcttctctg
      301 attagctata aggattgcga atatgcatct tgccctccga tctactccac ctcatagatc
      361 ttttccacca gatcgcagat cctctcccag tcgtagccct gtgccagctt caggcagggc
      421 tcacgcatcc tcgggctctc ttgaagtgcc aaaaggaggc cttctgccag atcctgggcc
      481 gcgggcgggc agataattcc ggtattttct gtgaccagat ccatcaccgc attcatcctg
      541 tgctcgacag tcaccaccgg caggccgcag gcattggcat ccagggcggc catgccgaat
      601 ccctctctgg tggagggtga ggcgaaggcc cgggaggact tcatcagggc caaagcatca
      661 tcatagttct ccagaaatcc gcagaactcg atatttttct ccagccccaa tttctctgtc
      721 agcgatctca gcctctccat ctccggcccg tcgccaatga tcgccgcccg cacatccggg
      781 agctctgact tcattatgct caccgcctgg atcaggaggt ggatgttctt atgggcggcc
      841 agccttccgg caaaaacgat atcagaactc tttggggagg gcgctatccc ctcaatcttt
      901 tgccagtcga tgccgtttgg caccacctgc acccctcttg cccccaggcc ctccagatcc
      961 ctggccgtcc tcttcgatac tgccagattg cgatcggtca gccgggcagc gatcaattcc
     1021 gtgatcctgc ccatctctcc cttccttccc aggtactcat gccagtaatc tttccagacc
     1081 tcatgccagg tgataaagag cttctggttc ttcttcctcc aactgctcaa gagacgagcg
     1141 gaaaagcagg acagataagg aaaattctgg cagtcgacta cctcctcctg ggaaggatga
     1201 gcaagcagcc tggcggcaaa gtaggctgct tctccaatcg atctctttcc attgacataa
     1261 aggggcctgg gctcgcatat gccatgaaag cgaacgccct ctctttcgat ctcgtcctct
     1321 ccctgccacc atttcatgcc gtagcagtgg acgacatgcc cgcgcgccgc cagccttcgg
     1381 gagagctcat agatcctctt ctccgcccct cctttgaccc agggatagac ggcatcgtac
     1441 acataagcga tctccaccta ttattcgcca tcctggtgcc cattgtaaag caatagcagg
     1501 ctgatgaaca tcccggagaa tatggtctga attccaagaa tggtgagtat catggccacc
     1561 atagcgttct gggcggcatc caatgcacca aagcccgccc ctctccacct catgaggacc
     1621 acgaggccaa tgacaattcc cgctgccagc agagccagcc ctattaagag ctctttttcc
     1681 agggaatggt agctgataag cttcttcatt ccgctcgact tggaaaggcc ccaggcagcg
     1741 ccaaaagctc tgaaatgcat cccggcaaga agcatctgat agccgatgat cagcagtagt
     1801 ccccccagga tcaaggaatg catcctcgag ccaccctcaa tataaaccac cactgccagg
     1861 atcaggccga ggaggagtgc taccaggccc gggacgagca gaaagggccc tggccggtag
     1921 agcatcatga atcgcatatg cctccagcca tcggtgaacg agttcagctt ggagtctccc
     1981 tggcggggat agtaggtaat gggaacctca gcaatcctca agccgcggga ggcggcctcg
     2041 atcaccatct ccgaggcgaa ctccattccc ccggacctga ggttcagctt atagagggca
     2101 tccctggtta tgccacgcag accgcaatgg gcgtcggaga ttccggcaga gaagagccgg
     2161 ttcaaaatcc aggtcaaaat aggatttcct acataacgat gaagggcggg catcgctcct
     2221 ggtatgatcc tgcctttgag cctcgatccc atgaccaggt catactcgcc gctctccagg
     2281 cagggaagca tatccaaaat ggtctcgggg tcgtaggtga ggtctccgtc catgagaaat
     2341 atatagtccc ctccagcctc ctggaaaccc aataggtatg cattgccata ccccaacctc
     2401 tgggggaata caaccttagc cccgcaggag gcagcgatat ccgccgtctg gtcggtggaa
     2461 ctgtccgcaa cgattatctc tccctcaagg cccgccttct cgaaggcctc cagagcccta
     2521 tggatacact ggcgtatggt ttgggattcg tttctggtgg ggatgatcag gcttatccgg
     2581 ggcacatttt cccttacttt agtttagctg ttaagccttt ccttttatag atcatctccc
     2641 ggaatccggg gattattttg agctcagagc gtctcgggct cgacctcatg cttaaaggag
     2701 agggtcaccc ttaccctgta ctccattatc cctccctctc taagcctggt gtccatcctc
     2761 gtgacctcgg caattcttac gttggtcagt tttcggttcg cagtattgat tgcagattgg
     2821 actgcgtcct cccagctttc atttgaggtt cccacgagat cgataaaatt gtacacgctc
     2881 ttgcctattg gggcttccat aacatattct atgctctaat gattgatata ttttgtctga
     2941 aaagaaaaga gattcgcaga tctattctgc cttcttctct tcgctcttct ctgcctcttt
     3001 gtccccttct tcggttgaat cttcagacgt ctgagccaca ggaggctgag cctctggctt
     3061 cttgggtccc ctgcccatgg cgatcaaaga ggcctttgcc tggatcctgg ccaggggata
     3121 gtcgtccttg gccaccttct ctaaagcagc caccgcccgg ttctccttca gctcaccgat
     3181 cacccaggcc gctcctgcac gcacatcctt gtctgcgtct tccagggcct cgatcatgcg
     3241 atcggatacc tgctttccat atccggcaat ggccaccatg gcattgctct ttaccagata
     3301 ctcctcgtct ttgagggcct caattaaagc ctcaagggac tcagatatat cccgtttaga
     3361 gaggagcatg atggcgttgg agcgcacaag catatgcttg tccttggtgg cggcgatcag
     3421 cttctctatg gaccagctca tgtcggggag gacggcaggc atggagggct ctgtcgctcc
     3481 gcaggttggg caggcctctt ccctcatgct ctgggtgatt acaggctctg ccttctcctc
     3541 agttttgttt tcttctggct tgttctcaac catttttttc tcttcttcca tagactggtc
     3601 tatctcccct attctacttc agcttttccc aatgcaaatg ccctcttatt ggcctccaga
     3661 tgctttggcg gcacaacctc gaccagggct tggatgatat gctcctctga gaggggaata
     3721 tactgggaaa gggcgccgag catcaccacg ttcatggcct gagcggttcc cgccttccgc
     3781 gccagggcgg tggcatccag ggccaggacc ctctggcatt ggccgcaaag cggctctatc
     3841 agatcatcta aagagggata ccgggctctt ccggtggtga cagttaccgg caggaccggc
     3901 tggttgttga tcagggccac cccttctggg gagaggaaat ggccaaatcg cagggcttca
     3961 gccggctcca gggcgatcaa gagatcggcg ctgcccggag cgaccaaggg gctgaagcgg
     4021 catcctattc gggtgtgatt gatcacgctc ccccctctct gggccatgcc gtgggtctcc
     4081 gcgcctcgca ccggctttcc cgccaggacc cctgccctgc cgatcacgtc tgatatcaat
     4141 atcactccct ggccgccgac gccgacgatc accatatcgc attctgaggt tctcatctct
     4201 ccacctccaa aatggcagag ctggggcaga tctgggcgca gatcccgcag ccggtgcata
     4261 gattgttgat ctttgccatc tctccctcaa actcaatggc cgggcagccg tactccacac
     4321 agatcctgca tccttgacac tcatcgctca cctggaaggc ccgtggccgt gtcccgcttc
     4381 tgcgggcctt gatcacgcag ggctgtctgg cgatgactac cgataggccg ggaaaatcct
     4441 tagccctctg gaatccctcc atcgtcccct caaggtcgta gggatccacg gtctcgacca
     4501 gccctgctcc caaagccctg gccagcccct ccagcgagac ctgggccgtg ggctcgccgg
     4561 tggcagtaac cccggttccc ggatgatgct gatggccggt catggcggtg gtggagttgt
     4621 ccagaattgt caccgtgatc ctggccttat tgtaagcggc attcagcagg ccggtcatgc
     4681 ctccatgcag gaaggtggag tctccgaggc ttgcgcagat cccctccgtc tcaccgccaa
     4741 atcttatgcc gcttgcgagg gtgatgctcg cccccatgca aagacaggta tcaaccgtcc
     4801 ccatgcccac tgccatggta tagcagccta tatcgctggg atagatcgcc ttcttgccat
     4861 agacccgtct catggcatag taggtggcac ggtggctgca gcctggacat aaagccggag
     4921 gccggggtgg gactatctcc gctgctgcaa aggatgctct ctccatatta cccaggccgt
     4981 tcatcctggc cagggcattt ctcaccgtaa taaggtccag ttctccctcg cgagggatct
     5041 ccttttcctt gcccaggatc tgtatggagg gatttatctt ccctgccagc atcgccacct
     5101 gttcttcgac caccggctcc agctcttcta tcaccagcac cctcgagaca tgctcgagca
     5161 tccggcatat cagcttcttt ggggcgggat aggctccaat cctgaggaga gtgtggtcaa
     5221 ggtccagatc catcagagcc tcctcggcat acagaccggc aatccccgag gcgatcacac
     5281 ccgtctcccc ccggagggtt agcctgttcc aggggcaccc ctccagctcc tcttctattg
     5341 ctggctgctt ggccaaaagc tcgtggtgga gcggccgggc atggcttggc agggccaccc
     5401 gtcgcgcagg attcttgacg aaacggcgct cgactggaat tggtcgatcc tggctcacct
     5461 ccacgtccgc ccgtgcatga gagacgcggg tggtgggccg gagcattacc gggatgttga
     5521 atttctccga caactccagg ccatagatcg ccatatcccg ggcctcctgg ggatcggcag
     5581 gatccagaca gggaatggag gcgaactggg cgaagcgtcg tgagtcttgc tcattctggg
     5641 atgaatggca gaaggggtcg tcggcacaga tcaccagcag acctgctcct acccccaggt
     5701 aggccagggt catgaagggg tcggcggcca cgttcaggcc cacatgcttc atggtggcca
     5761 cggagcggca gccggtccaa gaggcacctg ccgccacctc cagcgccacc ttctcgttca
     5821 cagaccattc tatatgtagg ggcagatcgc ctttcagctc caccagggcc tcaatgatct
     5881 ctgaggaggg agttcccggg tagccggcgg caagtccgat ccccgcctcc agcaggcctt
     5941 tggcgatagc tgcatttccg aggagatatt ctctcacaaa gaaccatcct tttaaatata
     6001 ttttaattat attctttctc tgaaaggatc aatgcccttc tctctgataa ccgttttgat
     6061 gccgctgccc ccgcgctcca gcccattcca caaaattgat aatccaaccc acctttcctg
     6121 caatcatgct ccagtggcaa tccgattctg tgggatcgat ctacaactac ctctcccctg
     6181 gctgccagat ctgtcggcag ggggcgggcc tggtcctctt cgtcactggc cggtgcgaac
     6241 ggggctgctt ttactgccca atctccgagg atcgaagggg aaaggatgtg gcatatgccg
     6301 atgagcagcc ggtgggagag ctggctgata tcctgagcga gggcagggcc atcggcgcct
     6361 taggcacggg gatcacggga ggagagcccc tcttaaggct ggactatgtc ttggattgca
     6421 tccgtgcatt gaaggaggag ttcggaagcg agcaccatat ccacctctac accggcattc
     6481 tgcccaacag atctgtcctg gagaggctgg taagagccgg cctggatgag atccgcttcc
     6541 atccgcctga taaggaatgg tctgatccag tcggactaaa ggaggtcctg gaggaggcaa
     6601 aggcattggg attgcaggct ggggtggaga tcccggcata taagccagcg ccacagatcg
     6661 ttcatgcggt ccgagaagca gatgcctttc tgaacctaaa tgagctagag ttctcggaga
     6721 ccaacttctc tcgtctaatg gaagagggtt ttttgcccct ggacctgggc tgcggtgcag
     6781 aaggcagcga agagatggcc cgaggctatc tattggatga catcaaggtg cattactgtc
     6841 cctcccgctt caaggatgct gtgcagctga gagagaggct gcgacgccgg gcggagagga
     6901 catcccggcc gctggactac atcacagaag agggaaccat aatccatggc ataatcgagg
     6961 gcaaggaggg tgatcttgag tcagcactgg ggatcatcga tgggctggag gtgcctgctg
     7021 aaatgtactc ctgcctggaa ggaaggatcg agatcgccgc ctggattctg gaggagatat
     7081 gcccggatct cgagggctgc aaatgcgatc tttgtataat tgagaggtat cctctgcagg
     7141 atggaccggt cgtggagaga atacctttat aatccttaaa tccattcttt cgaggcggtg
     7201 attttgccgg atggagagcg cggaagaacg aatccaggag agactgagga attatcttaa
     7261 gcgggatggc gtgggaatcc ggaaggcggt cttgaagctt ttcctgagcg atggtgccta
     7321 taccacggag gacgtcttca cctatctcgc gaaggagggc tttgacgtca gctaccgagg
     7381 ggtttcggcg atggtcgggc tcatgaacac tcgcctgggc attttgagca ttgatgtctc
     7441 tggggatcac aacgtttact ccctaaaggg ggaccacaaa ggagtggtga ggtctgtttt
     7501 ggagaattat tgatttaata tccccgaatc cggatgcgcg ctcggcaatt ccgacatgtc
     7561 aatggcaaaa tatttgtgtt tgcatatgaa tgactctagt actataatgg cggcaagatg
     7621 ctgatgctac aacgtctctt ccgcttctca gctccatatg ccaccgctag gcaggggctc
     7681 agttgggcgg ctcgcctctt ttcattctct tcagatattt attaagtgcg acgtctgcga
     7741 tcaagttcac tccggtcaag gcagcatgaa ccagcggccc tgcggtcatc ctttccttat
     7801 ataggctatg cagtctcttc aaatgtttga ggttgtactc cagacacaga cccaccagat
     7861 gccagtgctc gggcagggca tcggcctttg aaaaacctct ggtgccgccc agtttcgtct
     7921 cggcgatggt ggtgaaaaag aggggcagca ttaggccagt gtagtctttc agagactcca
     7981 ccagctcagt ggtccggatg acatcatctg ccgtctctcc aggcaggccc aagaccaggc
     8041 tggccacggg aagccaggat tcctcgtcga agaggcggta gcagtcccct acgatctggg
     8101 gccagttctc cgtctctcca ggaagcgcct tttggggcat atgcatcttc aggatgcggc
     8161 agcttcccgt ctctatccct atccaggcag acatgtggtt ctgatccgag cctatgccca
     8221 cgatcttgga gatctctctt aatagaccct ggttggagtg gaccgaggcc aggctgagat
     8281 gggagaagtc catagtcttc ggccccagct tcttgacctc tgccacgagc tcagtcatct
     8341 tcttttcatt cggccttgtg cctttggagc cataggagaa gacgtcctcg ctgtggagga
     8401 tgatatcctc ttggccgcat tccaggttca cccgcacgtc tttaagaata ttttcaatag
     8461 gcctgtggcg cacagagcgc atggtgggcg agcagaatgc gcatcctctc cagcagcccc
     8521 tgccgatctc cacaatcccg ccaatggtcg ctcctctgtt taccggtata ttttctccag
     8581 gcaccgccga tccccgcaac acctctggaa cgctctctcc tcttatgatc tgccggcaga
     8641 tctcgggaaa atcgctctcg ccctctccca ggtagacgtg gtcgacatcc accttctccc
     8701 ccatgacctc ccaggcgccg tttccaccca ggaccactcg tggccggtgc tcttttatca
     8761 aggggtggct caaaagctcg ataaaacggg agcggttgaa gggcgggccg cggcggatga
     8821 tatcctctat ttcccggagg gcgatctttc ccatggggtc atcatgggtt atccccacga
     8881 tcctggtctc cggtccgatg gcccggtcca ggtgaagggg atgggccacc atcacctcat
     8941 ccctggagaa tccggactcc aggagtgccg cttccacctt tctcatgccg cagggggcag
     9001 ttgccgccga tccgtcctcc agcgccggca ctggcgggca gaagaagttc tggaagatcc
     9061 aatcgggcag ggttgccctg ggcatgatgg cagcaaagcc gagcatcact ccgccatgat
     9121 agctgctcat catggtggta tcgctggtga gaacgattct cttccctgga aattgcgcca
     9181 tctatcgacc tctgattctc ttttggcaga taacgttatt ggcagtgcta gcccaataag
     9241 tttttatcgg atcgcccccc ctttgccgat gtgcaagaga agtcgttatc ggagataaac
     9301 gaacgcattc gcaacggctc tgcccgagtc gtcacggcag aggagatgcc agatatcgtg
     9361 gatgagctgg gcacggcggg agcggtgcgt gaggtggatg tagtcaccac tggtaccttt
     9421 ggcgccatgt gctcttctgg ggttttcctc aatttgggcc atagcgatcc tcccataaag
     9481 atcgcccgcg cctatctgaa cagggtggag gcctatgggg gcatcgccgc cgtcgacctc
     9541 ttcttaggag cgacccagcc ctctgaggac cgggggatcg agtatggcgg ggcgcatgtt
     9601 atggaggacc tggtaattgg aaagcagatt gaggtcgagg ctcatggatc tccctcggac
     9661 tgctaccctc agatggatct ggagaccagc ctcaggctgg aggacttcaa ccaggccacc
     9721 atggtcaatc cccgcaatgc ctatcagcgt tacaatgccg ctaccaactc ctccgaccgg
     9781 acaatgcaca cctacatggg caccctcctt ccgggtcttg gcaatgtcca ttacagcggt
     9841 gcaggggtgc tctctcccct ctccaacgat ccgggcttcg agtacatcgg ggtaggtacg
     9901 cgcatcttcc tggcgggagc tcagggctat gtcataggct cgggaaccca acatagtcct
     9961 gagaatggct tctccactct gatggtcacc ggcgacctaa agaggatgaa caacagcttc
    10021 ctgcgggcag caacatttca taaatatggc cccagccttt acctggggat aggaatcccc
    10081 ataccgatgt taaacgagaa gctcgccaag agcactgccg ttcaggacag cgatatcacc
    10141 gtcccggtgc aggactatgg catccagcgc agggataagc cctcggtgag gatggtcagc
    10201 tatgaggatc tgaagtcggg tacgatcgac ctggggggca aggaggtctc ctgctctcct
    10261 ctatccagct tccagatggc aagagaggtt gcccggacat taaaagagca ggttgagcgg
    10321 ggcgagttcc tgctcagcca ggcggctgaa cccctatcac gggtgggaag ctgccggccc
    10381 atgaagcaga ccaaggagtc gcccaatgtg ggagatgtca tgagcaggga tgtggttacc
    10441 gtctttgagg atatcccggt ggagaaggcg gcaaagctga tcatcagcgg cagcttcgac
    10501 catctgccgg tggtctcccg tgatggaagg ctgatcggca ttataactgc ctgggatata
    10561 tccaaagcgg ttgccagcgg aaaaccatcc cgcatagcgg agatcatgac ccgtcgggtc
    10621 cattcggtcc gcttggatga gccaattgag ctcgctgccc gcaccctgga tactcatagc
    10681 atctctgccc tgccggtggt ggacagagag cataaggtga taggaatgat aaccagcaat
    10741 catctctctc gcctattggc ccggaggcga tagccttgaa gctgatgctt catgtggctc
    10801 cgggcattgt ccgccatccc ctcatcgcct cagtgattct ggagactgag gctctggtta
    10861 acatcgagag ggcgagcatc aatgcagtga gcggcgagat agtcctggag gttccgcctg
    10921 agaagtgcca ggaggtaatg gaggcttttg agaggcgggg agtaagggtc gttcctctgg
    10981 agatcccggt gatcagggat gagtccgaat gcgtgaactg cggtgcatgc gtatccatat
    11041 gccccaccgg gacgtttcgc tttgaggatt ggcgggtggt cgccgactcg ggaaagtgca
    11101 ttcaatgcgg ggcctgcgtt gtgggctgtc cgcagagggc cctaaagctg gtcttgaagt
    11161 agctccccaa acgggagctg agctttttgt cttttgtttt atatgttaat tcaaatatta
    11221 atattaaggt gcacacattt taggattaac attatgaaga aatagaagaa tttatatggt
    11281 cggacagtat atagtggtgt ggtatgtcgg ccgaagaatt ctaaaacgat tgtggggatg
    11341 acataatatg ttatttgatg tattcaggca tgcagcagtc ctcaaatcga ataaatatcc
    11401 ttcaatttcc catcctatct gggggggtga atatggactc gagctctgag agcgtcgtct
    11461 cctccctcgg ctcattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttg
    11581 ccgagatgct ctccgatccc gagggctacg atcccgagag gctgaagagc ctatccgttg
    11641 gcgttccatc tttattatcc tctttcattg aggccatgct ctcctccgat gaggacgtta
    11701 tgaaaaggag cctcaccgac ctggaggatt atgcctctgt ggtcaacagc gttcaggcat
    11761 ggttcaatct gggaaggctt gctcaccatc tcttctttca tcaggcggca attgaatact
    11821 acagcaatgc ttcagatctg gcccagattc tctctgatga tgattcattg gccagtatcc
    11881 tcctatcctt gggagccctc tacggtgagg aggaggactg ggcccgggcc agtcagtgtt
    11941 atgagaaggc tttggttgct atcgatatgg ttaaaaggcc acgtcttttg cctcaggtcc
    12001 tggagaggct gggccgcgcc cgccgccagc agggtgagta ccatcaggcc cagatatgct
    12061 actcccgcat cctggaggtg ctcgacccaa gggacaaaaa agggcggatg gaggctacaa
    12121 gcaacctggc agagctctgc cagattcaag gcgatatgga aaaggcggag aagctgtatc
    12181 agaccattct gaaggagagg gagctgtcgg atgatcccgt gggggcagct cgggctcttg
    12241 cctctctcgc taacatctac tctcagaccg gcaacggcaa gagggcccag atcagcctgc
    12301 aatcagccag ccatctgctg gccgatctga aagatcaatc cggagcggca gagatgcagt
    12361 gccgtcttgc tgatatctat tttcatgagg gaaggctaaa ggaggctctg gagctatatg
    12421 gccagagctt ggcggcattg cagaagggcc atccctctct cgcctctaag gccctctgcc
    12481 gtatgggtca atgcttcttg gaggacggga attggatcct ggcagaggac caccttcaaa
    12541 gggcaatatc gatcatgaaa gagcataaag attcctcctc ccttgcccgg gctctggtta
    12601 ttctggcagg tgttcaccat aagcaggggg agctggaaat ggccctgcaa tgctcccggg
    12661 agagcctgaa gatcagggag ggatccaaag atcagagcgg atctgcagag gctctgaact
    12721 gcatggccag gatctgctgc gactgctctg attggccgga ggcgatcgag tgctaccaga
    12781 gggcggcgga tctcttcatg gaaatcggcg aggtgacagc agcgtctgag gccttatcca
    12841 atctgggcgg cgtatatcat ctgaaaggag atctggatgt tgccctgcag atttattctc
    12901 atgcctatga ttcattctgc cagcagggag acagacaggg cgcatcgatg gtccagtcta
    12961 acatcggcct tgtctatcag agcaggggcg atcttcacct tgctggggag ttcttggaaa
    13021 agagcattgc cacaaaggag gatctggggg atcaaattgg cgctgccggc aacaggctga
    13081 gcctggggct gatctatcag ctcggagggg agtgggatca ggctcagtcc tgttttgaat
    13141 gggcggccag ggcatttgag gataatgggg acctgcgggg tctggctgca gcggagaaca
    13201 acctgggaaa cctctactcc gatcgagggg atctcatgca tgccatctta tgctaccgtc
    13261 agggcctgga ggcgaggcgt gttctgaacg atgggcacgg catggcagtt acccttggaa
    13321 atctggcagg cgtctatctt cagtctgggg aggaggagcg agcagaggaa ctgtaccagc
    13381 agagcataga tggcttcaag gagctgggtg acgctcgagg agaggctaga tcgctctatg
    13441 gcctggcaag gagcatggcc gagagaggcg atctggaggg agcactggag tgctaccgaa
    13501 aatgtctcga tctgcagatt ataggagggg actccaggtc gatgatcgag gcgctcgagt
    13561 ccatggtaaa tctgctcctg agaaggggta gatggcagga ggctcagacc aggtatgctc
    13621 aggttctgga ggccctccgt tccagagatg atctttcggc agtagcagca gtccagtgcg
    13681 ctctggggaa tctccatgcc gatcttggcc agtgggatga ggccctggag gactacaatg
    13741 agagcctggc gattatggag gggctggggg acgagtactc cgtttcggta gccaggaaca
    13801 acctgggcaa tatgtgctac cggcagggtg actgggccgc cgccttagag aactatcgct
    13861 tgagcctgga gggcttcggg aaggcagggg atatggagag tgaggcctcg gtcctgagca
    13921 atgttgcagg cctctactat aagaggggtc agtgggcgga tgctctggat agctatagca
    13981 agggcctgag cctctttgaa aagctggagg actcagctgg cattgcccag gtgctgggaa
    14041 acatgggaaa cctctaccac cgccaaggag agcgcgatct agcagcagag cactttcaga
    14101 gggctctgga gatataccgg cgcctcaagg atcaagcggg cgcggcggag atgcaggcga
    14161 gcatcgatat gatccgggca ggacggggcg agatttcccc tgatatcgag gagtacaaga
    14221 gacagctgga ggagctggag gagacaggcg acctctccgg ccagggggag gtcctttcag
    14281 ccctcgccgg cagccacaca gatagcggcc agtgggcaga agccattgcc tgctataaga
    14341 agagcctgga gagatttgac cgaactggag aggtctacaa ccgcggccag gttctcttca
    14401 atatggccct ggtctacaag gatatgggag acctcatccg ggccagaacg ctatttgatg
    14461 aggcgatgag catcttccag gatttagatg cccagccctg tatagcttca gccaaattga
    14521 acctgggaat catctccggc cagatgggca gatcggatga agcaattgag cttttctccc
    14581 agtcaatagg cattctggag ggcctggggg cattgcccga tctatgtgag ggttacatcg
    14641 ccgccgccct gttcaatatt caagaggggc attcgatgca atcccgcttc tatctctccc
    14701 aggcagagac tcaggtctct cgcactgact atccgccttt gaaaatccag ctttatagag
    14761 ctcagggaga gagctttttg aaggatatgc tctatccgga ggcaaagagc tgctttgaga
    14821 aggccctggc tttatcccgg aagctgtctc tctcctatga ggaggcaaaa gccatctcat
    14881 cccttggtcg gctagccctg gccagcgggg attatgagga tgctctaaga aggatgaaac
    14941 aggctcttgt gatcttcggc cggctgggtg catcgctgga tctcatagag gtctaccgcg
    15001 atatgaccct cctcttcctg gcccaggatg attatcagcg agcagaagag atggccatgt
    15061 tgagacagcg ccaggccagg attctgggac atgctgatct cagtctcctg gctaccgtcg
    15121 atctggcgga gtgcgaagcc cgcactggca agaaggagga ggcacgggcg gactatgccg
    15181 gtgcattgcg gctggccggg aaggaggggg atcggattcc ttcatccacc atccgccaga
    15241 tctcagaaaa ggcgatagag ttcctggagg ctgaagcgtc tcagccggag gacgaccgtc
    15301 aggccagcct cctgcgagag aggcttggag aagaggatta tgagaagctt ctggaggagc
    15361 ttccccataa gctggaatgc aggagaatgg gctagaggcc tgagcccatc atggagggga
    15421 gaaggctgag ctggaggtcg accttggcca ggtcctgcct tatattggat tccttgagga
    15481 gcagatcaag ttcagccagc ctgccgatca catcgcccag ccgggctccc ttgtgctcgc
    15541 tccgggagcg aactggtgca taatatatct ctgctcccga gccgttcacc tccactccaa
    15601 agaaatcctt gtcatagaag cccttgaact gcagaattat tggtttgtag acattcttta
    15661 tggccggggc gtagaactcc gccagcatca cctgcaacat tctgttcttg gtatcagcta
    15721 tcctgaggcg gacatatccc tggccatgga tataatttcc ataatgaatt ccatatttta
    15781 tgcgagaggt cagatccgtc ttcaggctaa atacgaactc acaggtatca tccgtctccg
    15841 ttgggtcgag gttgagaaag tacttgatga ggagattata gccgttttca aaggttatcc
    15901 ggatgctgga ctcatcgctc ttcgagccgc tgaccaggag gcctgaaaca tcccagccaa
    15961 agatgtttct tgcctcttcc tttgtaaaag agtgtgatgc aaactcttga acctttccca
    16021 ggagatcttg catggtttcc atttttattc ac
//
LOCUS       DAOSGP010000002        15649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_3078_length_15649_cov_17.670065, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000002 DAOSGP010000000
VERSION     DAOSGP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 15649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15649
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3078_length_15649_cov_17.670065"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLV49_00075"
     CDS             complement(<1..746)
                     /locus_tag="PLV49_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOG97266.1"
                     /translation="MKYPDSEKAFVEPAAVVLAGIEERYLACPDCPPEPNLDKTRGCR
                     DLPGRVKRCSSCGRATLDAVMLDALHVLHDFGLRDERETLRSVGSPLVAVGYPLAYSP
                     RLGPDSLIIQGENISREAAQAMVQRIPEIRGVILFQGVPGMHKKGTAPLENALLAGSD
                     LRADVCQSLFGELVIYKSQSKIHIEFSRQSAPKMRIIERLMLQGKASRVVDGLCGPGT
                     LGLMCALAGAREVVLNDVWLPAVQNVLLNL"
     gene            858..2123
                     /locus_tag="PLV49_00080"
     CDS             858..2123
                     /locus_tag="PLV49_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HOG97267.1"
                     /translation="MAQRGIREYDAKNLLARYLPEYLDDFSYKGNLALVGPETDIEEL
                     EAENPWLKTTRLVVKPDQLFGKRGKLGLVLLDADWEEAKEYLNEKMGLEVTIGGITGR
                     LSYFLIEPFTPHKEEYYVAISSDYEGDNIFFSMDGGVGVEENWEKVISIHVDSLEGID
                     ALDVGSKLPAELGDKRALVEEFITALWRFYSDTGFAYLEINPFTFLGREIVPLDMVAK
                     LDDAEEYWQKKRWSDLAFPEPFGRTPSKEELFIKEIDSKTGASLKLTILNPEGRVWTM
                     VAGGGASVIYADTICDLGHAYEMANYGEYSGDPNTEETYHYTCTILDLMTRSKNPKGK
                     VLLIGGAIANFTDVAKTFKGVVMALEEYQQKLQEAGVEIYVRRGGPNYEQGLKLMRDL
                     GKRLGVPIQVHGPETHMTRIVPLALEENQ"
     gene            2120..3988
                     /locus_tag="PLV49_00085"
     CDS             2120..3988
                     /locus_tag="PLV49_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOG97268.1"
                     /translation="MSRKDYVLFDRNTKAFVYGYQTNAIQRMLDFDYVCKRSSPSISA
                     IINPSRAGIHKAFWGTKEIILPMYKTIPLAALAYPEADVMVNFASHRSAFETTMEALQ
                     EDTIRIVAVIAEGVPERQSRVMAATARKLDKIVIGPATVGGMTAGAFRIGNTAGTIEN
                     IIASKLYRPGCVGFVSKSGGMLNEAFNIISRNSDGIYEGVAIGGDRYPGSNMLDHILR
                     YERNPAIKMIACLGELGGEDEYMIIQALKEKKITKPLVAWVTGTCSPYLPASVQFGHA
                     GAKANTEKETAQAKNDAFRQAGAYVPRSFDDYGEMVRQVYDMLLIQGIVQKFEEPEVP
                     RIPTDYSKALATGDIRKPTTFICTISDDSGEELLYAGKKLSDVLDRKMGIGGVIGLLW
                     FKKELPEYAAHFIELVIQIVADHGPAVSGAHNAIVASCAGKDLISSLCSGLLTIGPRF
                     GGAIDDAAREFKRAQETGLAPEQFVGEMKKKGINIPGIGHKIKSVKNPDKRVQLLISY
                     ARANFPSTELLNYALQVEELTTAKKGNLILNVDGCIGILFIDLMSSCGAFSKEEIDEV
                     VRLGYLNGLFALGRSIGLIGHILDQKRLGSRLYRHPAEDIAYMMPSEEEIQCKRDR"
     gene            4056..5303
                     /locus_tag="PLV49_00090"
     CDS             4056..5303
                     /locus_tag="PLV49_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOG97269.1"
                     /translation="MATISQKILSRASGYDGMAEPGEIVEAEIDYAMSHDGTSVLAIR
                     SFREMGSEKVWDPDRVVVPYDHIVPANNETAADLQREVRNWAREQNISHFFDCGTGVC
                     HQVFPEQGFALPGTLLVGADSHSCTYGAFGAFGTGVGATDMAEIYSAGRLWFRVPETI
                     GIKVEGRLAPFVSAKDIALQVIGDLGADGATYKSVEYIGPAIEGLSISGRMTLCNLGV
                     EMGAKAAIVPPDEKTDEWLRGRARQPYARVFSDPDSYQQQYDYDIAELEPQVAAPFRV
                     DNVRPVSELAGLKIDQAFIGTCTNGRLEDLEAAARILKGKRVRARTLVIPASREVLLD
                     ALKRGIIQTLVEAGAMIGPPGCGPCLGAHMGVLAEGEVCVSTSNRNFPGRMGKGGLVY
                     LASPATVAASALQGRLAVPGVSK"
     gene            5300..5965
                     /locus_tag="PLV49_00095"
     CDS             5300..5965
                     /locus_tag="PLV49_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG97270.1"
                     /translation="MRFDLHVHSNCSDGRDDIRTILRAAARRDLDGLSITDHDTLRGS
                     QQAMKIIREDKLDLILIPGAEVTTSEGHLLVLGIEDLPPRGLGPEETTDLAREQGGIA
                     IVPHPYHPFRHAIGRIPECDAVEVYNSKHLFGIANARALIGAKRRHLPAVAGSDSHFA
                     ATVGLGVTEIEAADAFEAIEAIQAGKTRIIGKRTPPKFFIGNTFKSIYLILTTGGGRR
                     RKA"
     gene            5994..6356
                     /locus_tag="PLV49_00100"
     CDS             5994..6356
                     /locus_tag="PLV49_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP-dependent riboflavin kinase"
                     /protein_id="HOG97271.1"
                     /translation="MRGRVVGGLGQGQYYISRKGYRHQFSHKLGFVPFPGTLNIKLDE
                     PFNPGPHQILIEGFREEERSFGECKCYKIKLNGLEGAIIRPDRSSYPPDLVEIISPLR
                     LRDELRLDDGDIVELILQ"
     gene            6579..>7112
                     /locus_tag="PLV49_00105"
     CDS             6579..>7112
                     /locus_tag="PLV49_00105"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG97272.1"
                     /translation="MNYSQNGSYPLALERINLSLEIDDELAEAWLLKGQILFQLGYLQ
                     EAIRSLDQVLRIDQSLDEAWSLKGEIMMETGRYRMAQLCFDSALRLDPGNMTLYNRLA
                     QSQLMLEDYDHALRSYKKALSLEANNTEILFNQGDLFLTLARYPEALNSFTLLLEQNK
                     SDILALKGRAECYRQLGQ"
     assembly_gap    7113..7212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7213..8692
                     /locus_tag="PLV49_00110"
     CDS             <7213..8692
                     /locus_tag="PLV49_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG97273.1"
                     /translation="SDILALKGRAECYRQLGQVDRAEEDYYTVLEKNPEDFESWRGLG
                     MVRYATEDYSSSLQAFDQALKINPHDIQTILGKGDLLLAMGNYSSALESYSAALLLAK
                     DNVSALYGKGLALSSLGREKEANECYRRILELEPDNIMALQKIADDLLERNESIQAAE
                     HYGLILGQDPKNARALLGMAKAQLDLGDLDQALQSYEELLGHDSNSSAAWIGRGEILL
                     LQTNIDPAIESFNRALDIEPQNPDALMGLAEALHQNGKLEEARSYYEAALAEEPSVRG
                     YRGLGNILCAQGEYGQSIPLFESALSQEPSDTESLMGKGLALAATGNSSGALQCFNEI
                     VSLNPEDSAAWSNRGSIFAALGRYDEARESLQKAAGISSSSADVWYNLGQVYRLMDRH
                     NQSRQAFENATRLSPDDPVLWLELGLAQERTGEAKLALKSLQRAVVLDPENEFAQYSL
                     ALALAGQGRFQEALQAFERVLEINPKNDLAKYSINVTSSQLY"
     gene            complement(8772..9239)
                     /locus_tag="PLV49_00115"
     CDS             complement(8772..9239)
                     /locus_tag="PLV49_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97274.1"
                     /translation="MRIRIEIEDGGFKSSHEFESQSIDKEALRDKIIGFLSASGIFDS
                     KDERGAIAPARDYRSSGTLMDRLEHFIKFEFSNTWFTSQELRERYETVADDIKLSTVS
                     TYLSRMNRDGLLEKRGNRNNRQYRLIEMIEPELIPSHNYQRQNSYEKRGAEGR"
     gene            complement(10585..10707)
                     /locus_tag="PLV49_00120"
     CDS             complement(10585..10707)
                     /locus_tag="PLV49_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97275.1"
                     /translation="MDEDLSEVFQLIAEDERRRRYLKEVGDWLIDEYFELHQAS"
     gene            complement(10717..11559)
                     /locus_tag="PLV49_00125"
     CDS             complement(10717..11559)
                     /locus_tag="PLV49_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG97276.1"
                     /translation="MTTTESEPTTESERTFSQLDIAPTVARLLGIQLPLNDGRAIDLV
                     EGWGCRNAAIIIVDSLGYDLLMHLLPCLKNTSRLIDDGILLRAKAVSNHTTPAIASIL
                     CGLVPEHHKIFDKAGAKASSILSLPEIASAAGLRSAVIMETNGAEVYEGLIEIIHGIS
                     DRIPPEEFDREACSMTLQSLREGPRLLVSYFIGIDKSVHMGREWSGIRSAVLHIDRCI
                     GDITEEADEKTLLIICGDHPIHAGPLKRSSEPYYVALILGRGRSKDNKEQNIDSIGLN
                     AASE"
     gene            11677..12087
                     /locus_tag="PLV49_00130"
     CDS             11677..12087
                     /locus_tag="PLV49_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97277.1"
                     /translation="MMIKKTSQIIVAALLILVMGDAIASNLNIDYSHNVAGTGTVMTN
                     FEMGSPENTKAIGEVHGTGEILNKYIFSTNSSENITILDQFLFTKAQPSNVTTIESYP
                     VMERMPGSFRLLGTSWSGQINAERISSSPRAKDL"
     gene            12112..>12340
                     /locus_tag="PLV49_00135"
     CDS             12112..>12340
                     /locus_tag="PLV49_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecJ"
                     /protein_id="HOG97278.1"
                     /translation="MAKGADAMDISLKLDELKELCKDCTDFICRQKEILVVSHVDADG
                     LTAAAIICTALDRQNIDYKPLFFRQLDENALE"
     assembly_gap    12341..12440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12441..13712
                     /locus_tag="PLV49_00140"
     CDS             <12441..13712
                     /locus_tag="PLV49_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOG97279.1"
                     /translation="NIDYKPLFFRQLDENALEEVADQGADLVIFTDLGSGMIQEILDR
                     NLQAIVADHHKPKACPAQPLAHINPHLVGADGANQLSGSGSAFLLARALASSPGANDD
                     LAALAVVGAVGDLQDMASGQLIGLNRYILDIGSRAGAVHFSRDIKLFGKQTRPVYKML
                     EYSQDPYLPGLSGKEDACIAFLKEVGISLGGEKWRRWIDLSQEEKASLVTAIVQKGLR
                     NGISNIRLERLIGEVYILLKEREGTELRDASEYSTLLNATARYGHAGVGLEVCLGDRD
                     KAFDEAHTLLGQHRQNLVAGLKLVSKRGITPLNSIQYFDAGDAILDTIIGIVAGMSFQ
                     MADRSRPILAFAQNVEGDLKVSARGTQDLVRSGLDLAHALSLSAQAVGGVGGGHNVAA
                     GATIPLQAKQEFLEMMDRIVAEQLSYKNNIK"
     gene            complement(13746..14186)
                     /locus_tag="PLV49_00145"
     CDS             complement(13746..14186)
                     /locus_tag="PLV49_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97280.1"
                     /translation="MAGLSGLVILLVLLVPINADHYLMEIGSKSNPNAHLFSYDQKFR
                     ESTTVKNYPLQMTATKEFTDATRLSTKMELRTEANLTEIVIDADFIGIGRLGYLVLDP
                     EKGDLKHEQSRINHLFIGNFSIEEQIMVAKDHMNECGYGSLPCA"
     gene            complement(14215..15114)
                     /gene="dapF"
                     /locus_tag="PLV49_00150"
     CDS             complement(14215..15114)
                     /gene="dapF"
                     /locus_tag="PLV49_00150"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOG97281.1"
                     /translation="MKLAEISEVELKDDPFLPHSLGFAKLHGNGNDFILVDEISRELV
                     AESAKRRFAIACCRRSFGIGADGVLFLASSQRADLGMRLFQPDGSEAEMCGNGIRCLA
                     KYAWESGYVGQSFDVETLAGIIPIQVRERDGSFWARVDMGVPRFNRPSIPACGSGDLM
                     DEQMGEFRVCAVNTGVPHAVIFVDDLDIDIYEAAPPIRNCSVFPEGANVDFVKPGSSL
                     QIRTFERGVEGETFSCGTGAVASASVARRMGLVGDEVLVETKGGPLIINFEKDKAFME
                     GPAVTVYSAEMDEEYWWSLFREM"
     gene            15195..>15649
                     /locus_tag="PLV49_00155"
     CDS             15195..>15649
                     /locus_tag="PLV49_00155"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOG97282.1"
                     /translation="MDLLELDRSIQIAKEAVLGGADWIEAGTPLIKSEGMDSVREMKK
                     ALPGTKIVADMKTVDTGAMEVEMAAKAGADIVALLAASDNSTVEDALRAARKYGVQIM
                     MDLLTVPDPVGRSRELEELGVDYICVHVGIDQQMTGKDTIDFLKQIVKEV"
BASE COUNT         3782 a         4113 c         4133 g         3421 t
ORIGIN      
        1 tttaggttga ggagaacgtt ttgcaccgcg ggcagccaga catcgttcag tactacctcc
       61 ctggcgccgg ccagggcgca catcagcccc agcgttcccg ggccgcacag cccatccacc
      121 acccgagatg cctttccctg cagcatgagc cgctctatga tcctcatctt gggagcgctc
      181 tgcctggaga actcgatatg aatcttcgac tggctcttgt agatgacaag ctcgccaaag
      241 aggctctggc agacgtcagc cctcagatcg gatccggcta atagtgcatt ctccaggggc
      301 gccgtgcctt ttttatgcat tccaggaacg ccctggaaga ggataacccc tctgatctcc
      361 ggtatccgtt gcaccattgc ctgggcagcc tcacgggata tgttctctcc ctggatgatc
      421 aggctgtccg gccccagacg gggggagtag gccagaggat agcccacagc caccagcggc
      481 gatccgacgc ttctcagcgt ttcccgctcg tcgcgcagac cgaaatcatg caagacatga
      541 agggcatcca gcattaccgc atccagcgtc gctcttccgc aggaggagca cctttttacc
      601 cttccgggca ggtcccggca tcccctggtt ttgtccagat tcggctctgg cgggcagtcg
      661 gggcaggcta aatacctctc ttctatccca gccaagacca ctgcggcggg ttcaacaaac
      721 gctttttcgg aatcaggata cttcatattc ggccagtccc ctggaagtat ctcaatattt
      781 tcccgaaaca aataacttat cccggagaaa agtttgtaaa taagaaaagc attagggcgg
      841 cgaaggtggt ttttgacatg gcacaaagag gcatcaggga atatgacgca aagaatctgc
      901 tagctagata ccttcccgag tacctcgatg atttctcata taaagggaac ctggcattag
      961 ttggtcctga gacggatata gaagagcttg aggcagaaaa tccgtggctg aagacgacca
     1021 ggcttgtggt caagcctgac cagctctttg gaaagagagg gaagctgggc cttgttcttc
     1081 tggatgcgga ctgggaggaa gcaaaggaat acctcaatga gaaaatgggc ctagaggtta
     1141 ccataggagg aataaccggg aggctgtcct atttcctgat agagcctttc acaccccaca
     1201 aagaggagta ctatgtggct atcagctcgg attatgaagg ggacaacatc ttcttctcaa
     1261 tggatggcgg cgtcggggtg gaggagaact gggagaaggt aatatctatc catgtggact
     1321 ccctggaagg aatcgatgct ctggacgtag gctccaagct gccggcagag ctgggagata
     1381 agagagcgct ggtggaggaa ttcatcaccg ccttatggcg gttttatagc gataccggct
     1441 ttgcctacct ggagatcaat cccttcacat tcctgggccg cgaaatcgtc ccgctggata
     1501 tggtggcgaa gctggatgac gcagaagagt actggcaaaa gaagagatgg tcggacctgg
     1561 cattccctga accattcggc aggactcctt ccaaggagga gctcttcatc aaggagatcg
     1621 actccaagac cggagcctct ctgaagctca ccatcctcaa cccggaggga agggtctgga
     1681 cgatggtcgc cggcggcgga gcgagcgtga tctatgccga taccatctgc gatttgggtc
     1741 acgcctatga gatggccaat tatggtgagt acagcggtga ccccaatacc gaggagacct
     1801 accactacac ctgtaccatt ctggatctga tgaccaggag caaaaatccc aaagggaagg
     1861 tgctcctcat cggtggggcc attgccaact tcaccgatgt ggccaagacc ttcaagggag
     1921 tggtcatggc cctggaggag taccagcaga agctgcagga ggccggtgtt gagatctatg
     1981 tgcgccgggg aggtccgaat tacgagcagg gcttgaagct catgcgggat ctgggcaaaa
     2041 ggcttggcgt tcccatccag gttcacggac cggagaccca catgacgaga atcgtccccc
     2101 tggcactgga ggagaatcaa tgagcaggaa agattacgtt ctctttgaca gaaatacaaa
     2161 agcattcgtc tatggctatc agaccaatgc catccagcgc atgctggact tcgattatgt
     2221 ctgcaagcgc tcgtcgccca gcatctcggc catcataaat ccaagcagag ccgggatcca
     2281 taaggcattt tggggaacca aagagataat cctgcccatg tacaagacca tccccctggc
     2341 agctcttgcc tatccagagg cagatgtgat ggtcaacttt gccagccacc gatcagcttt
     2401 tgagaccacc atggaagcgc tgcaggagga tactattcgc atcgtcgccg tcatcgccga
     2461 aggcgtgcct gagaggcaat ccagggtgat ggccgccaca gccaggaagc tcgacaagat
     2521 cgtcatcggt ccggcgacgg taggggggat gactgcaggt gccttccgca tagggaacac
     2581 cgccggtacc atcgagaaca tcatcgcctc caagctatat cgaccgggat gcgtgggctt
     2641 cgtctccaaa tcgggtggca tgctcaatga ggcattcaat ataatctcca gaaactccga
     2701 tggcatctat gaaggagtgg ccatcggagg agaccgttat cccggatcga acatgctcga
     2761 ccacatcctc cgctatgaga gaaatccagc catcaagatg atcgcctgcc tgggcgagct
     2821 gggcggtgag gacgagtaca tgatcatcca ggccctcaaa gagaagaaga tcaccaaacc
     2881 tctggtggcc tgggtcacag gcacctgctc gccctacctt ccggccagcg tccagttcgg
     2941 ccatgccggt gccaaggcca acaccgaaaa ggagactgct caggccaaaa acgatgcctt
     3001 ccgccaggca ggagcatatg tgcccaggtc tttcgatgac tatggagaaa tggtgcgcca
     3061 ggtctacgat atgctcctca ttcagggtat agtgcaaaag ttcgaggagc cggaggttcc
     3121 ccgcatcccc acggactaca gcaaggctct ggccacgggc gatatccgca agcccaccac
     3181 attcatctgc acgatatccg atgacagcgg cgaggagctg ctgtatgcag gaaagaagct
     3241 atccgatgtc ctggaccgga agatgggaat aggtggggtc atcggcctgt tatggttcaa
     3301 gaaagagctc ccagaatatg ccgcccactt cattgagctg gtcatccaga tcgtggcgga
     3361 ccacggcccg gcggtctcgg gagcacataa tgccatagtt gcctcctgtg cgggaaagga
     3421 tctgatctca tcgttatgca gcgggctttt aaccatcggc cccaggtttg ggggggcaat
     3481 cgacgatgcc gcccgggagt tcaagcgggc acaagagacc ggcctcgccc cggagcagtt
     3541 tgtgggcgag atgaaaaaga agggtattaa catccctggc ataggccaca agatcaagag
     3601 cgtaaagaac ccggacaaga gagtccagct gctcatcagc tatgcccggg ccaacttccc
     3661 ctcgaccgag cttctgaact atgccctgca ggtagaagag ctcaccaccg ccaagaaagg
     3721 taacctgata ctgaatgtcg acgggtgcat cggtattctg ttcatagacc tgatgagttc
     3781 ctgcggcgcc ttcagcaaag aggagatcga tgaggtggtg cgcctgggat atctgaacgg
     3841 cctctttgct ctgggccgca gcatcggcct gataggtcat atcctggatc agaagcgact
     3901 cggctcccgg ctctaccgcc acccggcaga ggatatcgcc tatatgatgc cctcggagga
     3961 agagattcag tgcaaaagag atcgataaca gatgcctgag ataatggagg atcgccattg
     4021 aatgagagca taagcagcac tgtggagaaa gggacttggc gactataagc cagaagatac
     4081 tctcccgcgc ctccggctat gacgggatgg cagagcctgg ggagatagtg gaggcggaga
     4141 tagattatgc catgtctcat gacgggacat cggtgctggc catcagatcc ttccgggaga
     4201 tggggtcaga aaaggtttgg gacccggata gagtggttgt gccatacgat cacatcgtcc
     4261 cggccaacaa tgagaccgct gccgatctgc aaagagaggt gagaaactgg gcccgcgagc
     4321 agaatatctc ccacttcttc gactgcggca caggggtctg ccatcaggtc ttcccagagc
     4381 agggcttcgc ccttcccggc acactgctgg tgggagcgga ctcacattcc tgtacctatg
     4441 gcgcttttgg agccttcggc acaggcgttg gggcaacgga catggccgag atctactccg
     4501 ctggccggtt atggttcagg gtgcccgaga ccataggaat aaaggtggag ggaagactcg
     4561 ctccattcgt ctctgccaag gacattgctc tccaggtgat aggagatctg ggtgcagatg
     4621 gcgctaccta caagtcggtc gagtatatag gtcccgccat tgaaggtctg agcatatcag
     4681 gaaggatgac cctctgcaac ctgggggtgg agatgggggc taaggcggcc atcgtcccgc
     4741 cggatgagaa gacagatgag tggctccgtg gccgggcgcg ccagccgtac gctcgggttt
     4801 tctctgatcc ggattcatac cagcagcaat atgattacga tatagctgag cttgaacccc
     4861 aggtggcggc ccccttccgg gtggacaacg tccggccggt ctctgaattg gcggggctaa
     4921 agatagatca ggccttcata ggcacctgca ccaacggcag attggaggac ctggaggcgg
     4981 ctgccaggat actgaaggga aagagggtcc gagccagaac cctggtaata cccgcttcaa
     5041 gagaggttct gctcgatgcg cttaagaggg gaatcatcca gactctcgtg gaagcgggtg
     5101 ccatgatcgg tcctccggga tgcggtccct gcctgggagc ccatatgggg gtcttggcag
     5161 aaggagaggt gtgcgtctcc acctccaacc ggaacttccc aggaagaatg ggaaaaggag
     5221 gattggtcta tctggccagc cctgctacag ttgccgcttc cgctctccag ggccgtctgg
     5281 ccgtgcccgg ggtttctaaa tgaggtttga tctgcacgtc cattccaact gctcagatgg
     5341 acgggacgat atcaggacta ttctgagggc tgccgctcgc cgggacctcg acggcctgtc
     5401 gattaccgat cacgatacat tgagaggatc ccagcaggcg atgaagatca tccgagagga
     5461 taagctggat ctgatactca tacccggagc agaggtcaca acctcagaag gccatctgct
     5521 ggtcctgggc attgaggatc tgcctcccag agggctcggc ccggaggaga ccacagacct
     5581 ggctcgcgag cagggaggaa tagcgatagt cccccatcca tatcacccct tccgccatgc
     5641 catcgggcgc ataccggagt gcgatgctgt ggaggtctac aactccaagc acctcttcgg
     5701 catagccaat gcccgggcgc tgatcggagc aaagaggcgc caccttcctg cggtcgcagg
     5761 gagcgactcg cattttgccg ccactgtggg cctcggagtc acggagatag aggcagcaga
     5821 tgcattcgag gcgatagagg ccatccaggc gggaaagaca aggatcatag gcaagagaac
     5881 acctcccaag ttcttcatcg ggaatacgtt caagtcgata tacctgatcc tgaccacggg
     5941 aggaggacga aggagaaagg cctaaattct ttgcggagga gagaaccacg gtgatgcgag
     6001 gaagagtggt gggcggactg ggccaggggc agtactatat ctcgcgaaag ggctatcgcc
     6061 atcagttctc ccataagctg ggatttgtgc cctttcccgg cacgcttaac ataaagctcg
     6121 atgagccctt caatcccggt cctcatcaga tcctcataga gggatttcga gaggaggaga
     6181 ggtcttttgg ggaatgcaag tgctataaga taaaattaaa tggcctggag ggggcaatca
     6241 tccggccaga caggagctct tatcctccgg atctggtgga gatcatatct cccctgcggc
     6301 tgcgggatga gctgaggctc gatgatggcg atatagtgga actgatactg caatgatact
     6361 catggcgggg aaaaaaactc ctcttttgaa ggttaatttt atcccataag cgatgacaaa
     6421 tgatcctgat gccaatggat aaacgctcct tccggaatgg gatcctgtca atctgccttc
     6481 tcttgacctg tgctttgatc ctcttcgaat ccgctcctac ctcagccacc accggcggta
     6541 cctccatccc taactctgac ttttatttga gagaggctat gaattacagc cagaatggct
     6601 catatcccct ggccttggag agaatcaacc tatcgctgga gatcgatgac gagttggccg
     6661 aagcatggct gctcaaagga cagatcctct tccagctggg gtatcttcaa gaagcaattc
     6721 gctccctcga ccaggttctg aggatagatc agtccctcga tgaggcctgg agcctgaaag
     6781 gagagataat gatggagaca ggaaggtaca gaatggccca gctatgcttc gacagcgccc
     6841 tgcgcctcga tcccgggaac atgaccctct ataaccgcct tgctcagtcc cagttgatgc
     6901 tcgaggacta cgatcacgcc ctgagaagct ataagaaagc gctgagcctg gaggccaaca
     6961 acacagagat cctgttcaac cagggagatc tgttcctcac cctggctcgc tatcctgagg
     7021 ccctgaatag cttcaccctc ctgctggaac agaataagag cgacattctc gccctgaagg
     7081 gcagagcaga atgctaccgc cagctcggcc agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nngagcgaca ttctcgccct gaagggcaga gcagaatgct accgccagct
     7261 cggccaggtc gatagggcag aagaggacta ttatacggta ctggagaaaa acccggagga
     7321 cttcgaatca tggagaggac tgggcatggt ccgctatgcc actgaggatt acagctcctc
     7381 actccaggcc ttcgatcagg cccttaagat caatccccat gatatccaga cgattcttgg
     7441 caaaggcgat cttctcctgg ccatggggaa ctattcgtca gctctagaga gctattccgc
     7501 tgccctcctg ctcgcgaaag ataacgtctc cgccctttat ggaaaagggc tggctcttag
     7561 cagtctgggc agagaaaagg aggcaaatga gtgctaccgc cggatcctgg agctggagcc
     7621 cgacaacatc atggccctgc agaagattgc agacgacctt ctggagagga atgaatcaat
     7681 tcaagcagca gagcattatg gcctaattct gggacaggac ccgaagaacg caagagcgct
     7741 gctgggaatg gcaaaagcgc agttggatct cggggacctc gaccaggccc tgcagtctta
     7801 tgaggagctt cttggacacg attctaatag ctccgccgcc tggataggaa gaggagagat
     7861 tctcctattg cagacgaata tagatccggc tatagagagc ttcaatagag cattggatat
     7921 cgagccgcaa aatccggatg ctctcatggg attagcagaa gctctccatc aaaatggaaa
     7981 actggaagag gcccgcagct actatgaggc cgctttggcg gaagagccca gcgtccgggg
     8041 ctacagagga ctggggaaca tcctctgcgc ccagggggag tacggccaat ccattcccct
     8101 ctttgaaagt gctcttagcc aagagccaag tgacaccgag agcctgatgg gaaaagggtt
     8161 ggctctggca gccacaggca atagctctgg agccctgcag tgctttaatg agatcgtctc
     8221 cctcaatccg gaggactccg ccgcttggtc taatcggggg agcatttttg ctgccttggg
     8281 aagatacgat gaggcaagag aaagcctgca aaaagcagct gggatcagtt catcctcagc
     8341 agacgtctgg tacaacctgg gccaggtgta cagattgatg gacagacata accaatccag
     8401 gcaagccttc gagaatgcaa ccaggctatc tcctgatgat cccgttctgt ggctggagct
     8461 gggcctggcc caggagagga ctggagaagc taagctggct ctaaagagcc tccagagggc
     8521 cgtcgtcctc gatccggaga atgaattcgc tcaatactcc ctggccctgg cacttgctgg
     8581 gcagggccgt ttccaggaag cactccaggc cttcgagaga gtgctggaga taaacccaaa
     8641 gaacgatctg gctaaatata gcatcaatgt gaccagtagc cagctttatt gattcagagc
     8701 agaaaggccg gaattacagt ggaaacccgg aggcggtgga aaagggttga tgctcttccg
     8761 gccaatattt tttatctacc ctctgctcct ctcttctcat agctgttttg gcgctgataa
     8821 ttatgacttg gtatcaattc aggctcgatc atctcaatca gtctatactg cctgttgttt
     8881 ctgtttcccc ttttttccag cagaccatcg cggttcattc gcgatagata agtagagact
     8941 gtgctgagct tgatatcgtc cgctacggtc tcataccgct ccctcagctc ctgagatgta
     9001 aaccaggtat tggagaattc gaatttgatg aagtgctcta ggcggtccat gagagtgccg
     9061 ctgctgcggt aatcacgtgc aggcgctatt gctccccttt catctttgct atcgaagata
     9121 ccggaagctg agagaaagcc tatgatctta tcccttaagg cctccttatc tatgctttgc
     9181 gactcaaatt catgagatga tttgaatccg ccatcttcta tctcaatccg gatcctcatg
     9241 ctctcctccg cttatccttc cagtagtatt gccatcagga tatcaattcg aaagctggga
     9301 gggttgattc ttcaccagag gagcaatacc gcttgagatt gatgcccttg ctgtctctaa
     9361 gcctggagag cctcgattga tcagatttct ccaggttcat atgcaatgta aatagcttga
     9421 tctgtcctct caattgtatc cccctccgca aatccatctc gcttcgttct gctctctgtc
     9481 acttgaccgg atcagcaaga ttgctcacct tcctgatccg atccacagct tctcgtgcca
     9541 tcccttcatc cgacagatag gctgagcaag accatttgcc tggtctccct ttaaacaagg
     9601 tcaaacttcc cttgctcgca taccttgcct tgctgcacct cgatcccagc tctgccaaca
     9661 gctgctcatt gccgtccctt tccagccacg agcatcttga tctatccagg cagtggatca
     9721 tcccatcgcc cgttcacaag ccgtcccttc ccagctgtga acagtcattc agaaagatga
     9781 tcaaccagga tccttatgca ctccttcttg ctaccccttg tgccatttgt atatgagaag
     9841 cggactaatt gtgaagcaat tatacaacca tcgaaagacc attttgagat catcgatatc
     9901 cccttcattg ctactcccga tgccatccct attggtagac gttccagtcg gattgtggtg
     9961 agcttgccat caagaacttt tagccctacc acaattgttg tgatggagca aatcgctccg
    10021 atgccccaga tatgctcact cgacactgct tctacttctc agatatactg caaaggttca
    10081 tccatcccct cagacgaata agccctccgt ccgccctgtc aatatgtatt gagacaggtt
    10141 gtctagccat tttggcacaa tgtaaacctg gttttagtgg tatataaaac tttccaatag
    10201 tcgatagttg tagttagttg cttaaaacat ctattaaagc ttatttttga gaataaatcc
    10261 agaatagtag tcagaatagt atttcacaca atggccacat gactgagaaa tcttgttaaa
    10321 actggttgtg aaaagtgcgt tcaggcgatt actactatca taaaagatat cagtaggcat
    10381 catctttttg cgcggaggtt tttattctct gtgaaaccat ttcacaatgg cagccaatat
    10441 cgaaaatttt agaagaagac tcatgagacg attggtttga taaaaaaatt tttagaagtt
    10501 gtgcgcgcgg gacgcgcggc atagaggcga taatgtgata ggagatgatt gatgagattt
    10561 atgatgtgaa atattgtgaa ttgtctaaga cgcctggtgg agctcgaagt actcatctat
    10621 caaccagtct ccgacctctt taagatatct gcgtcttcgc tcatcttcag caattaattg
    10681 aaaaacttca gagagatcct cgtccatcag agcatgttat tcgcttgcgg catttaaacc
    10741 tatcgaatct atgttctgct ctttattatc cttggatctt cccctcccca ggatcagagc
    10801 aacgtaatag ggctcagagc tcctcttcaa tggcccggca tgaataggat ggtctccgca
    10861 aataatcaac agtgtctttt catctgcctc ttctgtgata tcgccaatgc agcgatcgat
    10921 atgcaaaaca gcgctacgaa tgccgctcca ttcccgaccc atgtgcacgc ttttgtcgat
    10981 tccaatgaaa taagagacga gaagcctggg accctctcgc agggattgca gggtcatgct
    11041 gcaggcctct cgatcaaact cctccggagg aatgcggtcc gatattccat ggatgatctc
    11101 gatcagaccc tcgtagacct cagctccgtt tgtctccatt atcaccgcag atcgcagacc
    11161 tgcagcgctg gcgatctcag gcaggctgag gatgcttgag gcctttgccc ctgccttatc
    11221 aaagatctta tgatgctcgg gcacaaggcc gcagagaatg ctggcgatgg caggagtggt
    11281 gtgattggag actgctttgg ctctgagaag aattccatca tctattagac gagatgtgtt
    11341 cttcagacag ggcaagagat gcatcagcag gtcatatccc aggctgtcta ctattatgat
    11401 tgcggcattc ctgcagcccc agccctccac cagatcgatt gccctgccat cattgagagg
    11461 caactggatt cccagcaatc gagcgacagt gggcgctata tcgagctggc tgaatgttcg
    11521 ctcggattcg gtagtcggct ctgattcggt ggtggtcatg aacatcaatg cctcctacta
    11581 tctcaactca tataatatcc cacccagtct gcctggttaa tagcagacag ctcctcgtcg
    11641 gtcttaaata agaaaagaga aaacgcaaag gagaccatga tgataaagaa aacctcccag
    11701 atcatcgttg cagcgttgtt gatcctggtt atgggagatg ctatagcatc gaatttgaac
    11761 atcgactaca gtcataacgt tgccggaacc ggcaccgtca tgaccaattt cgagatgggc
    11821 tctccggaga acaccaaagc cattggagag gttcatggaa caggtgagat attgaacaag
    11881 tacatattct ccaccaacag ctctgagaac atcaccattc tggatcaatt cctgttcaca
    11941 aaagcacaac catccaatgt taccaccatt gaaagctatc ccgtgatgga gagaatgccg
    12001 ggcagcttca ggcttctggg aacgagctgg tcgggacaga ttaatgctga gaggatatca
    12061 tcttcgcccc gggccaaaga cttataaatt attcatcaaa tagaaagtga catggcaaaa
    12121 ggggcagatg ccatggacat ttccctgaag ctcgatgagc tgaaggagct atgcaaagac
    12181 tgcacggatt ttatctgcag gcaaaaggag attttggttg tctcccatgt agatgcagat
    12241 gggctcaccg ccgctgccat catatgcacc gccctggata ggcagaacat cgactacaag
    12301 cccctcttct tccggcagct tgatgagaac gctctggagg nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn aacatcgact acaagcccct cttcttccgg cagcttgatg
    12481 agaacgctct ggaggaggtt gccgaccagg gtgcggatct ggtcatattc accgatctgg
    12541 gcagcgggat gatccaggag atcctggaca ggaacctgca ggccatcgta gccgatcatc
    12601 ataagcccaa agcctgccca gcacaacccc tggcccacat caatcctcat ctggtgggag
    12661 cagatggagc aaaccagctt agcggcagcg gtagcgcttt tcttctggcc agagccctgg
    12721 catccagccc tggcgctaac gacgatctgg cagctttagc ggtggtgggg gcggtgggag
    12781 acctgcagga tatggccagc ggtcagctta tcggtctgaa ccgctacatc ctggacatag
    12841 gctcaagagc gggagcagtc catttctcca gagacatcaa gctctttggt aaacagaccc
    12901 gcccggtcta caagatgctg gagtactctc aggatccata ccttcccggc ctctcaggaa
    12961 aagaggacgc ctgtattgct tttcttaagg aggtgggaat aagcctgggt ggagagaagt
    13021 ggaggcgctg gatagatctc agccaggagg agaaggcctc attggtcaca gccatcgttc
    13081 agaaagggct gcgcaatggc atttccaata tcaggctgga aaggctgatc ggagaggtct
    13141 atatcctgct gaaggagagg gagggaaccg agctcagaga cgcctctgag tactccaccc
    13201 tgctgaacgc caccgcccgt tatggtcatg ccggggtggg gctggaggta tgcctaggag
    13261 atagggataa ggccttcgat gaagcccata ccctcctggg ccagcaccgc cagaacctgg
    13321 tggctggctt gaagctggtt tcaaaaaggg gaatcacccc attgaacagc atccagtact
    13381 tcgacgccgg agatgcgatt ttggatacga taataggaat cgtagccggc atgagcttcc
    13441 agatggcaga ccgcagccgg cccatcctcg cctttgctca gaatgtggaa ggggatctca
    13501 aggtatctgc ccgggggacc caggatctgg tccggtccgg cctggacctg gcacatgcct
    13561 tatccctctc cgcccaggcg gttggaggag taggcggagg ccataacgtc gccgccggag
    13621 cgaccattcc tctgcaggca aaacaggaat tcctggaaat gatggaccga atcgtggccg
    13681 agcagctttc ctacaaaaat aatataaaat aaaatatata cagtacgaat tatttcagtt
    13741 aacactcatg cgcagggaag agagccataa ccacactcat tcatatggtc cttggccacc
    13801 attatctgct cctcgatgct gaagttgcct atgaagaggt ggttgatccg cgattgctca
    13861 tgcttcaaat cccccttctc aggatccagg actagatagc ctaagcgtcc aatgccaatg
    13921 aaatccgcat cgatgacgat ctcggtgaga ttggcctctg ttcgcaattc catctttgtc
    13981 gagaggcggg tggcatcggt aaactccttg gtcgccgtca tttgcagggg ataattttta
    14041 actgttgtgc tctcgcgaaa cttctggtca taggagaaga ggtgggcatt aggattgctc
    14101 ttgctgccta tctccatcaa ataatgatct gcattgatcg gaaccaggag gacaagcaga
    14161 atcaccaacc cggagagtcc cgccacgatc cagcctcttg ccgctatgat attatcacat
    14221 ctccctaaat aggctccacc agtactcttc atccatctct gcagaataga cagtcactgc
    14281 cggcccttcc atgaaggctt tatccttctc aaagttaatg atcagcggcc cacctttcgt
    14341 ctccaccagc acctcatccc cgaccagccc cattctgcgg gcaacgctgg cagaggccac
    14401 cgccccggtt ccgcaggaga atgtctcgcc ctctacccct cgctcgaatg ttctgatctg
    14461 cagtgatgag cccggcttga cgaaatcgac gtttgctccc tccggaaaaa cgctgcaatt
    14521 ccttattggt ggtgccgctt cgtagatatc tatgtccagg tcatccacaa atataacagc
    14581 atgaggaaca ccagtattca ctgcacagac cctgaattcc cccatctgct cgtccatgag
    14641 atctccagaa ccgcaggcgg gaatggatgg acgattgaac ctgggaacgc ccatatccac
    14701 cctggcccag aagctgccat ccctctcccg gacctgaatc ggtattatgc ccgctaaggt
    14761 ctccacatca aaggattgac ccacataccc cgactcccag gcgtacttgg caagacagcg
    14821 tatgccattg ccgcacatct ccgcctctga gccgtccggc tgaaataatc tcatgcccag
    14881 gtccgcgcgc tggctgcttg ccagaaacag gacaccatct gcacctatgc cgaagctacg
    14941 acggcagcaa gcgatcgcaa atctcctctt cgcactctca gccacgagct cccttgagat
    15001 ctcatccacc agaatgaagt cgttgccgtt cccatgcagc ttggcaaagc caaggctgtg
    15061 tggtagaaag ggatcgtctt tgagctctac ctcagaaatc tctgccagct tcattttata
    15121 taccatgcat cctttttcta aagcttgtta tgttgtggtg gtgatttggt attaaaccca
    15181 ttctacaggt ggcaatggat cttctggagc tagacagatc gattcagata gccaaagagg
    15241 ccgtccttgg tggagcggac tggatcgagg ccggaacacc cctcatcaag agcgagggca
    15301 tggactctgt gcgcgagatg aagaaagccc ttcccgggac gaagatcgtg gcggatatga
    15361 aaaccgtcga caccggtgcc atggaggtag agatggcggc caaggccggg gcagatatag
    15421 tggccttgct tgcagcatca gacaactcca ctgtcgagga tgccctgcgg gcggcgcgca
    15481 agtatggcgt gcagataatg atggatctgc tcacggtacc cgatccggta ggccgttccc
    15541 gtgagctgga agagctggga gtggactaca tctgcgttca tgtgggaata gaccagcaga
    15601 tgaccggcaa ggacaccata gattttctta agcagatcgt caaagaggt
//
LOCUS       DAOSGP010000003        14913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_3292_length_14913_cov_19.591062, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000003 DAOSGP010000000
VERSION     DAOSGP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 14913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14913
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3292_length_14913_cov_19.591062"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /gene="pyrG"
                     /locus_tag="PLV49_00160"
     CDS             complement(<1..1306)
                     /gene="pyrG"
                     /locus_tag="PLV49_00160"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HOG97283.1"
                     /translation="MRYIVVTGGVMSGLGKGITAASIGRLLMNRGYKVTAIKIDPYIN
                     IDAGLMSPFQHGEVYVLKDGGEVDLDLGNYERFLDVELTRDHNITTGKVYSTVIEKER
                     RGEYLGKTVQIIPHITEEIKKRIRQVSRDGGCEICLVEVGGTVGDIESMPFLEAMRQL
                     KYEESGNIFFVHVTLAPSTTDGEQKTKPTQHSVKVMRELGLQPDMIVVRCENPLLPET
                     KHKIAQFCDVPAEAVISAHNSDDIYKVPLQMEAEGIARYIMKEMRLFPLEEKKDWDQF
                     VRKMGEVSGSVKVAIVGKYTVGQQCADPMEDAYLSIRESLKHAGIEAGVRPEIVWIDA
                     EELEHGSADLILREADGILVPGGFGSRGTEGKIKAVQYAREMKVPYLGICFGMQLAVI
                     EYARNVCGLDGAGSTEFGDTPHPVIAILPEQEKIRQMGATMRL"
     gene            complement(1309..>1597)
                     /locus_tag="PLV49_00165"
     CDS             complement(1309..>1597)
                     /locus_tag="PLV49_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97284.1"
                     /translation="REPLGSVEGEENSTEQFESDQIRSEEEAGEVAEDEDGLRAIGAM
                     QIIPAVIFLGLVSALVLTFITESIPEFPGALVSSILIVTLIIYGAATLLKA"
     assembly_gap    1598..1697
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1698..2326)
                     /locus_tag="PLV49_00170"
     CDS             complement(<1698..2326)
                     /locus_tag="PLV49_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97285.1"
                     /translation="MDHIVRINEGSDTKTPIVLHLLPGEETLFNLVVINDGEPSDVSL
                     QASDPVFKAVRFRRSDDYVDGKQIIPIFARMPDDTDRLDGEIILKSSIGDSRVPITIL
                     RDSIVSKSDQLPGTQPEGIGRQSPSDRLGRIPSRARIRDIASAEEAQTEDGWEEERNP
                     DEDEIGTDGIDPEIIDPEGMDDGGEDEDRSPYESSEDLDTEFVPAKDGR"
     gene            complement(2475..2657)
                     /locus_tag="PLV49_00175"
     CDS             complement(2475..2657)
                     /locus_tag="PLV49_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HOG97286.1"
                     /translation="MKRDLLDILACPMCKGDLILEVFEENESEILAGKLCCRACNEIY
                     PIEDGIPNMLPPELRE"
     assembly_gap    2850..2949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3090..4217)
                     /locus_tag="PLV49_00180"
     CDS             complement(3090..4217)
                     /locus_tag="PLV49_00180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOG97287.1"
                     /translation="MAGSDDRKSSERALRDSRSKKAEENLLRLADSLYGQFQRGVVPH
                     ISLPSRTKGNIEFSTKDDVWIYGDQETMRSVKTVRGAKTLLKTVHLTELLIDEHLKNN
                     RGSTLREIYYISENWDIAKFHEQAESDRLIEDLEIVTDLHREDFHVRPEEDGAAVFGP
                     LRLKEQTRRGEKEMHCQEDVGEAGYQIPFNVDNIEFINHDARMVMAIETGGMYARLIE
                     NGFDEEYNAILVHIKGQPARSTRRIIKRLNTELNLPVVVFTDGDPWSYRIFASIAYGA
                     IKSAHLSDHLVTPKARFLGVQPSDIVDYNLSTDKLTDRDLQALKSELSDPRFANPYWE
                     KQIHLQIDLKKKAEQQAFAGKGLDFVTKTYLPDRLTEMGII"
     gene            complement(4265..6100)
                     /locus_tag="PLV49_00185"
     CDS             complement(4265..6100)
                     /locus_tag="PLV49_00185"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOG97288.1"
                     /translation="MEIAEELAKQQKAISVAEFFEKNRQILGFDSAPRALITCVKEAV
                     DNSLDACEDAGILPDIFVQIKKSGEYFQVIVEDNGPGIVPEEIPRVFAKLLYGSRFHT
                     LRQSRGQQGIGISAGVLYSQLTSGRPTRVISKIAPDRPAYYCELMINTSKNEPEIIKA
                     EEVDWERPRGTRVEMEMEGSYVRSRRQSVYGSLKSAAIVNPHARLTLVEPEGNVEVFE
                     RATESLPKKAYAIQPHPEGIELGELIKLLRYTDKKKLRVFLKETFSSIGTISAQELCK
                     KADLDPEAPPASLDHDEAVRLLLAFKQIRVKSPPVDCLSPIGEELIRAGLEKEFRLDF
                     IATTTRPVSVYSGNPFLVEVGLGYGGELAREGRIEILRFANRVPLVYQQGACAIAHAV
                     ESVSWKNYSLSQSAGSGIPVGPAVLLVHIASTNIPFTSESKDAVADMPEILVEIDLGL
                     KEVARKLRRYLSRQSLLSERREKEEIIRKILPRIARKLSDVLDRPEPDIGPVVAKIMG
                     NLLVYRESEQRDSRISVRLVVENHSDLVRSFKLHEILGEKAEAVSPPAKVISLGDGYD
                     HHWKLSVGPGQKAIISYQIGANSNGPAKPVVEGLDPEIVTGARAI"
     gene            complement(6120..6350)
                     /locus_tag="PLV49_00190"
     CDS             complement(6120..6350)
                     /locus_tag="PLV49_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOG97289.1"
                     /translation="MVIGVTMVKVVPGQEKTVYNALQEIDGIKDVYHVFGEFDFVVII
                     EVEGLSMLNKLVDVIREIDNVNATQTVVGAEL"
     gene            complement(6399..7289)
                     /locus_tag="PLV49_00195"
     CDS             complement(6399..7289)
                     /locus_tag="PLV49_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97290.1"
                     /translation="MAGFSREVARRIFAEELKSSNYSFREGEDQHQYAPQYLLTPTGA
                     KCNRVFFVGTLTEKDDIGTDVEYWRGRVVDPTGSILIYAGQYQPEAAQILASMDAPAF
                     VSIVGKPNLYQTEDGNVIISLRAESIQRVDEATRNQWIMDTARRTLERLSLLEKTEPA
                     PLSSDFATADSAYPSTNQQDAARAREHYHTDIERYRKMVARALASLKADLAKTVGDDS
                     GSLASSAGSEWMGEENGDELYEPIEEGFEAKREAKQKEMPEEKQPNKKIKPKKGGSLD
                     SWPEAGGDEEVETFNLGKKS"
     gene            complement(7291..8520)
                     /locus_tag="PLV49_00200"
     CDS             complement(7291..8520)
                     /locus_tag="PLV49_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein A"
                     /protein_id="HOG97291.1"
                     /translation="MKEIIDQISARLREQNILVPDEEIEVRLKKLIDDFRVPEGEARR
                     SVLNYFQKEHGIMPQARASSEKVKLSEIKESGRWVDLEVKVLDLWEPATEAISQTGLV
                     GDSSGAMKFVKWKKSDLPDLVLGQSYSLKKVITDEFQGRFSIKLNRTSQIEPLENEVE
                     ARSVSKAAQEMKVIEISEPGLWVDLKVKVAQLWEPNSDSISQSGLIGDETGSIKFVKW
                     TKADLANLEEGKSYLLRNLVTDEFQGRFSVKLNRTSQIEPLDVDIEIGSQAAEFSGAL
                     VDVQKGSGLIKRCPVCKRSLAKGVCTEHGKVDGTYDLRIKAVLDDGRRVQDVLINRET
                     TERLVGLTLDEARMMAMEALDHEVVRSLIESKLVGRYFAIAGPRVDRYLLVETINELM
                     PVTESSVDELMSRVEAI"
     gene            8734..9627
                     /locus_tag="PLV49_00205"
     CDS             8734..9627
                     /locus_tag="PLV49_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG97292.1"
                     /translation="MEIATWFGRIDSETRSVFLAEDGGRMIDALLKKPLPAGPSVQPD
                     LRRLALMHGYAADDIEYNARLREIALGLVRRQLAQLATTEQDLLQAVEAIDDMTEAIN
                     LLDERLYEWHRLHHQRIVHGKDLAESLCEDRIMGPFARSILRLMESRKSMEEEVSFRA
                     EELAPNLSALAGPILAARLISRAGGLSRLAKMPSSRVQVMGAEKSLFKHLDGRAPSPK
                     HGIIFRHPAVMGAPRRLRGKVARALAGKLALAARLDYYGASPSPDLAASLERRLNDIK
                     RRGGNRKESIRWAGERNEQQG"
     gene            9770..10306
                     /locus_tag="PLV49_00210"
     CDS             9770..10306
                     /locus_tag="PLV49_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97293.1"
                     /translation="MTEHSWIYNQGYSDDMRIYQTTSGLHGQKLVTGTRGTGTISRSI
                     DAHVYGGFEDGFNEIWANEWGVYQNKPSLYSEPITKSDLRNALCAKNYEVGSIYSESY
                     SEIRDLVKDTSVSQTNENSVYSIHTEADGTARVGARVQRSTSSTPAYIMTGSYQGYTN
                     MRMQLESGNASIMTLPCP"
     gene            complement(10358..>11151)
                     /locus_tag="PLV49_00215"
     CDS             complement(10358..>11151)
                     /locus_tag="PLV49_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HOG97294.1"
                     /translation="VTEATEPVIEWCPLFDKVRGIKKVTKESAAANMEFRIKEHGMFS
                     ARRKLEMDTFVGFGASESMMTGLMRDIIDAAVTVCDGAGTVITSNGSLVQGMGGYISG
                     LVETDPIPEVIKGIKSRDGHVLSPESGRIDQLEGAAYAISAGYCRFAVTVADAQTAER
                     LRELERSTGVRIMIIGVHLTGISKDDARRLLKAADIVTSCASRHIREEAVPLVQVGTA
                     VPLFGLTPWGKELLVERAKDVSSPLLINTMPLPVLPEKKQPRPLV"
     assembly_gap    11152..11251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11279..11689
                     /locus_tag="PLV49_00220"
     CDS             11279..11689
                     /locus_tag="PLV49_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOG97295.1"
                     /translation="MLSFIMRLVKALNTSASADIAAERDEFASILMKIGLKRNVAKVL
                     TYLAGVPEATSREIEIGSDLRQPEVSIAMREIRRLDWVAERDERNPGKGRPYRIYKLN
                     RSLPDIVQYLEAENAKEAERVMKQIEKLKSLKSN"
     gene            11981..13093
                     /gene="ftsZ"
                     /locus_tag="PLV49_00225"
     CDS             11981..13093
                     /gene="ftsZ"
                     /locus_tag="PLV49_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG97296.1"
                     /translation="MKYIIDDAMGRAEAESRVKPSSMIKSDEELVSVLEELTTVIRVI
                     GCGGGGSNTIDRLAECGIQGAELFAVNTDAQHLLHINADRRFLIGRRTTRGLGAGSLP
                     AIGEEAAQEDIDEIRAAVDGADMVFVTCGLGGGTGTGASPVVAEAAREAGALTISIVT
                     IPFSAEGTIRMQNAEAGLNRLREVSDTVIVVPNDRLLDVVPNLPLQAAFKVADEVLMR
                     SVKGITELITRPGLINLDFADVRTVMTNGGVAMIGMGEAQGEEKARDSVSKALRSPLL
                     DVDVSCATSALVNVVGGSDMTITDAETVVDEVYQKINPEARIIWGAQIDQNLDHTLRT
                     MLVVTGVSSPQILGKDMSKRVTSRHGIDFLSRRARY"
     gene            13310..13618
                     /locus_tag="PLV49_00230"
     CDS             13310..13618
                     /locus_tag="PLV49_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOG97297.1"
                     /translation="MISILTEKVSGELEMLQRHLLILKHVVENEPIGILKLAEETSIP
                     SHKVRYSLRILEQEGLIKATAPGATTTHETGPFLKELDSMIEELSKRADELKKIKIAR
                     "
     gene            13619..>14913
                     /locus_tag="PLV49_00235"
     CDS             13619..>14913
                     /locus_tag="PLV49_00235"
                     /inference="COORDINATES: protein motif:HMM:NF016553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97298.1"
                     /translation="MLYRQIAQSIEELSSAPRSCKAELCSRLIAEMKPELLCPAIRLL
                     LGQLWPPWEGREMGVGPEGIAAALEEISEENISRLRDRMGEMGLVAEAALAEKGQHSL
                     SSEPLQSMSVYEQLRRISLIRGKDSEQRKNALLRGLFQDASPLEGKYIARTVLGNMLA
                     GIGDKTMLAAFALAFCCDPDQIQRAYSLLPDPGKIAIMAAGNHLERASIQPGIPIKPM
                     TLCALKAEDLKTVREVGEESVEWAVLPRYHALRVQVHNTGDGAYIFSSRQKNITTSLN
                     GLAQRLSGMDDEFIIDADLIGFHEGKMCSQSDMLRYINRRRLSRRSSISPALLAYDLI
                     YISGEDTISLPFQERRKRMLEALGEPRSMPFQGISATREWIVLEKDLKKDSIQSHLDR
                     MVEEGGVGLMVRSLLGLYRPGECSRCDFFVGREMSISAV"
BASE COUNT         3175 a         4166 c         3864 g         3408 t
ORIGIN      
        1 ccagccgcat ggtcgctccc atctgcctga tcttctcctg ctcgggcaaa atagcaatca
       61 ccggatgggg ggtgtctcca aactcagtgc ttcccgctcc atccaggccg cagacgttcc
      121 tggcatactc gatcaccgcc agctgcatgc caaagcagat acctaaatag gggaccttca
      181 tctctcgggc atactgcacc gccttgattt tgccctctgt gccccgggag ccaaagcctc
      241 caggcaccag aatgccgtcc gcttcccgca ggatgagatc tgcagatcca tgctccagct
      301 cctcggcatc gatccagacg atctccggcc ggacgccggc ctcaatcccc gcatgcttca
      361 gggactcccg aatggagagg taggcatcct ccatcggatc ggcgcactgc tgacccacgg
      421 tgtacttgcc cacgatcgcc accttcaccg accctgatac ctctcccatc ttccttacaa
      481 actggtccca gtccttcttc tcctccagtg gaaagagcct catctccttc atgatgtacc
      541 tggcaatgcc ctccgcctcc atctgcaggg gcaccttgta gatgtcatca gagttgtggg
      601 cgctgatcac tgcctctgca ggcacatcgc agaactgggc gatcttgtgc ttggtctctg
      661 ggagcagggg attctcacat cgaaccacga tcatgtccgg ctgcagaccc agctccctca
      721 tcaccttcac gctatgctgg gtgggcttgg tcttctgctc cccatcggtg gtggaggggg
      781 ctaaggttac atggacgaag aagatgttcc ccgactcctc gtacttgagc tggcgcatgg
      841 cctccaggaa gggcatggac tcgatgtcgc ccactgtgcc ccccacctcg accagacata
      901 tctcgcagcc cccgtctcgg gagacctgac ggatgcgctt tttgatctcc tcagtgatgt
      961 ggggaataat ctgcaccgtc ttgcccagat actccccacg ccgctccttc tctatcactg
     1021 tgctgtagac cttgccggtg gtgatgttgt ggtctcgggt cagctcaaca tcaaggaatc
     1081 gctcatagtt ccccaggtcc aggtccacct ctcccccatc cttgagaaca tagacctctc
     1141 catgctgaaa ggggctcatg aggccagcat cgatattgat gtagggatcg atctttatgg
     1201 ccgtgacttt atagcccctg ttcatgagca gcctgccgat ggaagccgcg gttatgcctt
     1261 tcccaaggcc gctcataacc ccgcctgtga ccacgatata tctcattttt acgccttcag
     1321 gagcgtagcc gctccgtata tgataagggt gacgatcaaa atcgacgata caagggctcc
     1381 gggaaactct ggaatactct cggtgatgaa ggtcagaacc aatgcgctaa ccaggcccaa
     1441 gaagatgact gcaggaatga tctgcatcgc tccgatcgct cttaagccat cctcatcctc
     1501 agctacttca cccgcctcct cttcagaacg aatctgatcg gattcaaact gctctgtgga
     1561 attctcttcc ccttcgaccg atccgagtgg ctctcttnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnntcg cgtccatcct tcgccgggac gaattccgta tccagatcct
     1741 cggaggactc gtatggactg cgatcctcat cctccccgcc atcatccatg ccctcgggat
     1801 caattatctc tggatcaatc ccatctgtac cgatctcatc ctcgtcagga ttcctttcct
     1861 cttcccagcc atcctcagtc tgcgcctcct ctgccgaggc gatgtcccgg atccgagctc
     1921 gagaggggat cctgcccaac ctatcagatg ggctctggcg gccgatcccc tcgggctggg
     1981 tgccggggag ctgatcggac ttggagacta tggaatcccg caggatggtt atgggcaccc
     2041 ggctatcgcc aatgctgctc ttcaggatga tctctccatc cagccggtct gtatcgtcgg
     2101 gcattcgggc gaagatcggt atgatctgct ttccatccac atagtcatcc gatcttctga
     2161 agcgaaccgc cttgaagacg ggatcgctgg cctgaagcga gacatctgaa ggctctccat
     2221 cgttgatgac cacgaggttg aagagggtct cctcgccagg aagaagatgg aggacgatgg
     2281 gtgtcttggt atcgcttccc tcgttgatgc gaacaatatg atccaatccc aaaacccctc
     2341 actcgctatt gttacataat attgtataaa tatttcgcct gtgaatccca agccccttct
     2401 ctgcccccat ccaccattct ggggctctta gcgcggattc ggccaggcgt ccatctggtc
     2461 aatctagaat atgatcattc tcgaagctct gggggcagca tatttggaat cccgtcctca
     2521 atgggataga tctcattgca agcacgacag cagagcttgc cggctaatat ctccgactcg
     2581 ttctcttcga atacctccag gatcaggtct cctttgcaca tagggcaagc cagaatatcg
     2641 agaagatcgc gtttcatcgc tggcaacagc tgttcttata aagtataaat ctttgtctca
     2701 catccgaagc atccggtcct ggagacctga catgatggga atgggcttaa ttttaccagc
     2761 ggtcacttca ataaaatgga ggcattcctc cagagtatgg cctctttctc tccctctgat
     2821 agatccagcc tctggattat ctccactgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnc cggaacggct catgtgcccc aagaggtcct ggggcgctcc gcttcccggc
     3001 acctctacct tcagctttct tttggctgcg ggcaggatct tggctgccac cggcggagga
     3061 tagatatggg catgaaagtc gatgatcatt cagatgatcc ccatctccgt cagcctgtcc
     3121 ggcagatagg tcttggttac aaagtccagc ccctttccgg caaaagcctg ctgctcggcc
     3181 ttcttcttga ggtctatctg caagtggatt tgcttttccc agtagggatt ggcaaacctc
     3241 ggatcagaaa gctcgctctt cagggcctga agatccctgt cagtgagctt gtccgtggat
     3301 aggttataat ccacgatatc gctgggctgc actcccagga agcgggcctt gggcgttacc
     3361 agatgatcgg agagatgggc gctcttgatc gctccatagg ctatgctggc gaagatgcga
     3421 taggaccagg gatcgccatc ggtgaagact acaaccggga ggttcagctc cgtgttcagc
     3481 ctctttatga tccgccgggt ggacctggca ggctggcctt tgatgtgaac caaaatggca
     3541 ttgtactcct catcaaagcc gttttctatc agccgggcgt acattccgcc ggtctcaatg
     3601 gccatgacca tccgggcatc atggttgatg aactcgatgt tatccacgtt gaagggtatc
     3661 tgatagccgg cctcgccaac gtcctcctgg cagtgcatct ctttctcccc ccggcgggtc
     3721 tgctctttca ggcgaagcgg cccgaagaca gccgctccgt cctcctccgg acggacgtga
     3781 aaatcctccc ggtgcagatc ggttacaatc tccaggtcct cgatcagccg gtcgctctct
     3841 gcctgctcat ggaacttggc tatatcccag ttctcggaga tgtaatagat ctctcttaat
     3901 gtcgagcccc gattgttctt gagatgctca tctatcagca gctccgtcag gtggacggtt
     3961 ttaagaaggg tcttggctcc ccgaaccgtc ttgacgctgc gcatcgtctc ctggtctccg
     4021 tagatccaga catcgtcttt agtggagaac tcgatatttc ccttggtccg ggagggcaga
     4081 gatatatgag gtacaactcc ccgctgaaac tggccataga gcgaatcggc cagacggaga
     4141 aggttctctt ctgccttttt cgaccgggaa tccctcaacg ctctctccga gctcttcctg
     4201 tcatctgatc ctgccatgcc ctcactccaa tcgctgggat cctgtcatct atcagatctc
     4261 cctatcagat cgctctggcg ccggtgacga tctccgggtc cagaccctct accaccggct
     4321 tagctggccc gttgctattc gccccgatct ggtagctgat aatggccttc tggcccgggc
     4381 ctacggagag cttccagtgg tggtcgtatc catcccccag agaaattacc ttggcagggg
     4441 gactgaccgc ctccgccttc tcccccaaaa tctcatgcag cttgaaggat cttacaaggt
     4501 cgctgtgatt ctctaccacc agtcggaccg agatccggct gtcccgctgt tcgctctccc
     4561 tgtaaaccag aaggttgccc ataatcttgg ccaccaccgg accgatgtcc ggctcggggc
     4621 ggtccaggac atcggagagc ttccttgcta tccggggaag gatctttctg atgatctcct
     4681 ccttctcccg tctctcggat agcaggctct gcctgctcag gtagcggcgc agctttcgag
     4741 ccacctcctt gagccccagg tcgatctcaa cgagaatctc cggcatatcg gccaccgcat
     4801 ccttgctctc cgaggtgaag gggatattgg tggaggcgat gtgcaccagc agcactgccg
     4861 gaccgacggg aatgccgctt cctgcggact ggctcagaga atagttcttc caggagaccg
     4921 actccacagc atgagctatg gcgcaggccc cctgctggta gaccagaggg acgcgattgg
     4981 cgaatcggag tatctctatg cggccttctc gggccagctc accgccatag cccagtccca
     5041 cctcaaccag gaagggattg cctgagtaga cggataccgg cctggtggtg gtggcgatga
     5101 agtccagccg gaactccttc tccagccccg cccggatcag ctcctctcct atgggtgata
     5161 agcagtccac cggaggcgac ttaacccgga tctgtttgaa ggctaaaagc aagcgcaccg
     5221 cctcatcgtg gtccaggctt gcgggaggag cttcagggtc cagatccgct tttttgcaca
     5281 gctcctgggc ggagatcgtt ccgatggagg agaaggtctc cttaagaaat acccggagct
     5341 tcttcttgtc ggtatagcgc aagagcttga tcagctcccc aagctctatt ccctctggat
     5401 ggggctggat agcataggcc ttcttgggaa ggctctccgt cgcccgctcg aagacctcca
     5461 catttccctc cggctccacc agggttaagc gggcatgggg gttgacgata gcagcgctct
     5521 tcaatgagcc atagaccgac tgcctgcggc tgcggacata agagccctcc atttccatct
     5581 ctacccgcgt tccccgaggc ctctcccagt ccacctcctc agctttaatg atctcaggct
     5641 cgtttttgga ggtgttgatc atgagctcac agtaatatgc aggcctgtcc ggagcgatct
     5701 tggatataac tcgcgtcggc ctgccgctgg tgagctggga gtagagcacc ccggcggaga
     5761 ttcctatgcc ctgctggcct ctgctctggc gcagagtatg aaatcgagaa ccatagagca
     5821 gtttggcaaa gacgcgcggg atctcctccg gcacaatccc cgggccgttg tcctccacta
     5881 tgacctgaaa gtattcgccg ctcttcttta tctgcacgaa tatgtccggc agaatgcccg
     5941 catcctcgca ggcgtccaaa gagttgtcca ccgcctcttt cacacaggtg attaaggcgc
     6001 gaggcgcgga gtcgaatcca aggatctgac ggttcttctc aaaaaattca gctacggaga
     6061 tggccttctg ctgtttagcc agctcctcag cgatctccat aagaggggga tgacggggct
     6121 taaagctcgg ccccgaccac cgtctgggtg gcattgacgt tatcaatctc tctgatcaca
     6181 tcgaccagct tgttgagcat gctcaggccc tcgacctcaa tgatcaccac gaaatcgaat
     6241 tcgccgaaga catgatatac atccttgatg ccgtcgatct cctgcagagc attgtagaca
     6301 gtcttctcct gtcctggcac caccttgacc atcgttacac caattaccat attgactcac
     6361 ctctgctcta tgagcttctg tagattatat aagacttttc agctcttctt tccaaggttg
     6421 aaggtctcca cttcttcgtc ccccccggcc tcgggccagg agtccagcga tccacccttc
     6481 ttgggcttga tctttttatt gggctgcttt tcctctggca tttccttttg ctttgcttcc
     6541 cgcttcgcct cgaacccctc ttcaatgggc tcgtatagct catctccatt ctcttccccc
     6601 atccattcgg acccggcgga agaggcaaga gacccggaat catctcccac tgtcttggcc
     6661 aggtccgcct tcagagaggc aagggcccga gcgaccattt tgcggtagcg ctctatatcg
     6721 gtatgatagt gctcacgggc acgagccgca tcctgctgat tggtggaggg ataagcgctg
     6781 tctgcggtgg caaagtcgct tgacaatgga gctggttcag tcttctcgag gagtgagagc
     6841 ctctcaagag tgcgcctggc ggtatccatt atccactggt ttctggttgc ctcatccacc
     6901 ctctggatgg actcagcgcg cagagatatg atgacattgc cgtcctccgt ctggtagagg
     6961 ttgggctttc ccacgatgga gacaaaagca ggtgcatcca tgctggccag tatctgagcg
     7021 gcctctggct ggtactgccc ggcatagatc aggatgctgc ccgtcgggtc caccactcgc
     7081 ccccgccagt actccacatc tgtaccgata tcgtccttct cggtcagcgt tcccacgaag
     7141 aagacccgat tgcatttggc cccggtaggg gtgagaaggt attgaggagc atattgatgc
     7201 tggtcctcac cttcccggaa ggagtagttg gaactcttca gctcctcggc gaatatcctg
     7261 cgcgccacct ctctggaaaa tcccgccatc ctagatcgcc tccacccggc tcatgagctc
     7321 gtccacactc gattctgtca ccggcatgag ctcatttatg gtctccacca atagatagcg
     7381 atcgaccctt ggcccagcga tggcaaagta tctgcccacc agcttgctct cgatgaggga
     7441 gcgcaccacc tcgtgatcga gcgcctccat ggccatcatc ctggcttcat ccagggtcag
     7501 ccccaccagc cgctcagtgg tctcccggtt gatgaggaca tcctgcaccc ggcggccatc
     7561 gtccagcacc gccttgatgc gcagatcata tgtgccatcc acctttccgt gctcggtgca
     7621 tactcccttg gctaaggagc gtttgcatac cgggcagcgc tttatcaatc ccgagccctt
     7681 ctgcacatcc accagagccc cggagaactc tgccgcctgg gagccgatct ctatgtcaac
     7741 atccaacggc tcgatctggc tggtgcggtt cagcttcacg ctgaacctcc cttggaactc
     7801 atctgtgacc aggtttcgaa gaaggtagct cttcccctcc tccaggttgg caagatccgc
     7861 tttcgtccat ttcacgaact ttatagagcc ggtctcatcg ccgataaggc ccgactggga
     7921 gatggaatcg ctgttcggct cccagagctg agccaccttg accttcagat cgacccatag
     7981 tccgggctcg ctgatctcga tcaccttcat ctcctgcgct gccttgctga ccgatcttgc
     8041 ctccacctca ttttccaggg gctctatctg actggtgcga ttcagcttta tgctgaaccg
     8101 gccctggaac tcatccgtga tcactttctt cagagaatag ctctggccta agaccagatc
     8161 gggcagatcg gacttcttcc atttaacaaa cttcattgcc cccgaactgt cgccgacaag
     8221 gccggtctga gagatggcct cggtcgccgg ctcccagaga tccagaacct tgacctccag
     8281 atccacccac cggccggatt ccttgatctc ggagagcttg accttctctg agctggccct
     8341 ggcttgaggc ataattccat gctccttttg gaagtagttc aggaccgatc ttcttgcttc
     8401 tccttccggc acgcgaaaat cgtcgattag cttctttaaa cgaacctcaa tctcctcatc
     8461 agggacaagg atgttctgct ccctgagacg agccgatatc tgatctatta tttctttcat
     8521 tacccgtatg ccccctgttt ttcattgaga ggtgatagta acttggctct tgaactattt
     8581 aaggaagatc tgtgcagaga gaaagtaatc tccggagatt tcataaacaa gacttgcagc
     8641 tttcagaaaa cgggatgaaa tggaagagga taatatagat taaagagaag ataaagagaa
     8701 aacaaatggc ttattgccgg gaggtatggt tgtatggaga tcgcgacctg gtttggaagg
     8761 attgattcgg agactagatc agtattctta gctgaagatg gggggaggat gatagacgcc
     8821 cttttaaaga agcctcttcc tgccggccca tccgttcagc ccgacctgcg ccgcctggcc
     8881 ctaatgcatg gatatgcggc agatgatatc gaatataacg cccgcctcag agagatcgcc
     8941 ctgggcctcg ttcgccgcca gcttgcccag ttggctacca cagaacagga cctcctccag
     9001 gcggtagagg ccatagacga tatgactgag gcgataaatc tgctggatga gaggctctat
     9061 gagtggcatc gcctgcacca tcagaggatc gttcatggaa aggatctggc ggagtcgctc
     9121 tgcgaggatc ggattatggg accctttgcc aggtccattc tccgattgat ggaatcgagg
     9181 aagagcatgg aagaggaggt gagctttcga gcagaggaac tcgccccaaa cctctccgct
     9241 cttgccggac cgattctggc agcgaggcta atttccaggg caggaggcct gagccggctg
     9301 gcaaagatgc cctcctcccg ggtgcaggtc atgggagcgg agaagtccct cttcaagcat
     9361 cttgacggcc gcgccccttc ccccaagcac gggataatct tccgccatcc agcagtcatg
     9421 ggagccccaa gaaggctgcg gggaaaggtg gcacgcgctc tggctggaaa gctcgccctt
     9481 gccgcccggc tggactacta tggagctagc ccctcacctg acctggcagc ctccctggag
     9541 aggcggctga atgacatcaa acgcagggga gggaatagaa aggagagcat cagatgggct
     9601 ggagaaagga atgagcagca aggatagaat caggatcaaa tttatattcg attgagatga
     9661 agttgcgcat atgaaatgtt ggcagtccgg gtttctgatg atatctgcgc ttagtatcat
     9721 cgcctgcttg ggccttctgg ttcccccggc agcgggagtt gctcagaata tgactgagca
     9781 ctcctggatc tacaatcaag gatactcgga tgatatgcgc atctaccaga caaccagcgg
     9841 cttgcacggc cagaagttgg tcactgggac tcgaggtaca ggcacaatat cccgctccat
     9901 tgacgctcat gtctacggcg gatttgagga tggattcaat gagatctggg ccaatgaatg
     9961 gggagtctac cagaacaagc catctttata cagcgagccc atcaccaaga gcgacctgag
    10021 aaatgccctc tgcgccaaga actacgaggt gggatcgatc tattccgaga gctactctga
    10081 gattcgggac ctggtcaaag acacctcagt gagccagacc aatgagaact cagtgtacag
    10141 catccacacc gaagcggatg gaacggcaag ggtgggagcg cgggtgcaga gaagcaccag
    10201 ctccactcct gcctacatca tgaccggaag ctatcagggc tacacaaata tgaggatgca
    10261 gctggagagc ggcaacgcct ccattatgac ccttccctgc ccatgaggca gctatatttt
    10321 tcagagtctc tttgctgagg attccattat ccaagatcta gaccagaggc ctgggctgct
    10381 ttttctccgg gaggacagga aggggcatgg tgtttatcag cagcggcgaa gagacatcct
    10441 ttgccctctc caccagcagc tcctttcccc agggggtcag gccgaagaga ggcaccgcgg
    10501 ttcctacctg caccaggggt actgcctctt ctcggatatg cctggaagcg cagctggtga
    10561 cgatatccgc cgccttcaag agccttctgg catcatcctt gctgatccct gtgagatgga
    10621 ccccgattat cattatccgc actccagttg atctctccag ctccctcagc ctctctgcag
    10681 tctgggcatc tgctaccgtc acagcgaacc ggcagtatcc agcgcttatg gcataggccg
    10741 cgccctccag ctgatcgatc cttccactct cgggagagag gacatgccca tccctggatt
    10801 ttatgccctt tatcacctct gggatggggt ccgtctccac cagtccggag atgtatccgc
    10861 ccatgccctg gaccagcgat ccattggagg tgatgaccgt tccagcgcca tcgcacacgg
    10921 taacggcagc atcgataata tccctcataa ggccggtcat catcgattcg gatgccccaa
    10981 agccgacgaa tgtgtccatc tccagcttcc ggcgggcgga gaacattcca tgctccttaa
    11041 tgcgaaactc catattggca gccgcagact cctttgtcac cttttttatt ccccggacct
    11101 tatcgaaaag aggacaccat tcgatcaccg gctcggtggc ttccgttacc tnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaattattt ttgacgaaac ttatatatat
    11281 gttatcattc attatgcgcc tggtgaaagc tttgaataca tcagcatctg ctgatatcgc
    11341 tgccgagcgc gacgagtttg catccatatt gatgaagata ggcctgaaga ggaacgtggc
    11401 caaggtcctg acctatctag cgggagtgcc cgaggcaacc agccgggaga ttgagatcgg
    11461 atccgatctg cgccagcctg aagttagcat tgccatgaga gagattagaa ggctggactg
    11521 ggttgcagag agggatgaga ggaatcctgg aaaaggtcga ccttatcgca tctataagct
    11581 gaaccgcagc cttccggata tcgtgcagta tctggaggcg gagaatgcaa aggaagcgga
    11641 gagggtgatg aagcagattg agaagctcaa gtctttgaaa tcgaattgat tttatatatc
    11701 aaaggaaaaa ggtgcgcccg agctggctcg atgtgaaatc ggcatacaga gctattacaa
    11761 aagtttattt ttaatatttt gatattaatt aagtaatgaa attttctgat atgcatgcag
    11821 atgattttca catacttcaa gttgattatg cataaccaaa ttcaaatgca ctcacctaaa
    11881 catctatata atatttcaag atgcgccaat ttactctatg caaaaatcta tatactaata
    11941 gattactaga atatggcaga ataaagaaga ggggatggat atgaagtaca tcatcgacga
    12001 tgccatgggc agggctgagg ctgagagcag agtcaagcct tccagcatga ttaagagcga
    12061 tgaggagcta gtgtcggttc tcgaggaact gaccacggtc atcagggtaa ttggttgtgg
    12121 tggaggaggc tcgaacacca tcgaccgcct ggcagaatgc ggcatccagg gggcagagct
    12181 ctttgcggtg aacaccgatg ctcagcacct tttgcatatc aatgccgatc gccgtttcct
    12241 gatcggcagg cgtacaactc gcggcctggg cgcgggcagc ctgcctgcca tcggtgaaga
    12301 ggctgctcaa gaggatatag atgagatcag agcggcagtg gatggggcgg acatggtctt
    12361 cgtcacctgc ggccttgggg gcggcacagg cactggcgcc tcgccggtgg tggcagaggc
    12421 ggcccgtgag gcgggcgctc ttaccatctc catcgttacc attcccttca gcgcagaggg
    12481 gacgataaga atgcagaatg cagaagcggg cttaaatagg ctgcgagagg tctcggacac
    12541 cgtgatcgtg gtgccaaacg atcgactttt ggatgtcgtt cccaaccttc ccctgcaggc
    12601 ggccttcaag gtggcggatg aggtgctgat gcgctcggtc aaggggatta ctgagctcat
    12661 aacccgtccc ggtctgatca acctggattt cgccgatgtt agaacggtga tgacaaacgg
    12721 cggagtggct atgatcggaa tgggtgaggc acagggagag gagaaggcgc gggactcggt
    12781 atcgaaagcc ctgcgaagtc ccctcctgga tgtggacgta tcctgcgcga catccgccct
    12841 ggtgaacgtg gttgggggtt ctgacatgac catcaccgat gctgagaccg tggtggatga
    12901 ggtctatcaa aagataaatc ctgaggctcg catcatctgg ggagcacaga tagaccagaa
    12961 cctcgaccat actctgagga ccatgctggt ggttacagga gtctcgtctc cccaaatact
    13021 gggaaaggac atgagcaaga gggtgacctc aaggcatggc atagacttcc tatcccgccg
    13081 ggcacgttat tgatggagct tacataggag gtgccactgc ctccctatag ctctgccttc
    13141 cccttttgct cgcccatatt tgcatctttt ctcgttttct ttcgattgcc cctcaaagcc
    13201 cgttgctgca tttgatcatc tctgttagcc gtgctagcat gcaccatgtc aatcgatgga
    13261 attataagtg cgctccccct atcagtatct gtttatctat tgaagggatg tgatatccat
    13321 tctgaccgag aaggtgagcg gggagcttga gatgttgcag aggcacctgc tcatactcaa
    13381 gcatgtggtg gagaacgagc ccattggtat tctcaagctg gcggaggaga cgagcatacc
    13441 cagccataag gtgcgttatt ctctaagaat actggaacaa gagggtctga tcaaggcaac
    13501 cgctcccggc gcgaccacaa cccacgagac cggtccattc ctcaaggagc tggactcgat
    13561 gatcgaagag ctttccaaga gagcagatga actgaaaaag atcaagatcg cgaggtaaat
    13621 gctctatcgc caaatagccc agtccataga ggagctatcc tctgccccgc gcagctgcaa
    13681 ggcggagttg tgctcccgcc tgattgcaga gatgaaacct gaattgctct gccctgcgat
    13741 ccgtctcctg ttgggccagc tctggccgcc ctgggagggc agagagatgg gggtggggcc
    13801 agaggggatc gctgccgctc tggaggagat ctcggaagag aatatatccc ggctcagaga
    13861 tcgaatgggg gagatggggc tggtggcgga agctgcactg gcggaaaaag gccagcattc
    13921 gctctctagt gagcctctac agtccatgtc cgtatatgaa cagctaaggc ggatctcttt
    13981 gattcggggc aaggactccg agcagagaaa gaacgctctt ctccggggcc tcttccagga
    14041 tgccagtccc cttgagggaa agtatatcgc tcgcacagtt ctaggaaata tgctggccgg
    14101 aatcggcgat aagaccatgc ttgcagcttt tgctctggcc ttctgctgtg acccggatca
    14161 gattcagagg gcctatagcc tcctgccgga cccgggcaag atcgccatca tggctgccgg
    14221 aaaccatttg gaaagagcgt ccatccagcc gggcattccc ataaagccga tgacgctgtg
    14281 cgccctgaag gcagaggatc taaagaccgt aagagaggtg ggagaggaat cagtggaatg
    14341 ggcggtttta cccaggtatc atgccctgag ggtgcaggtt cataatacag gagatggggc
    14401 ttatatcttc agctcccgcc agaagaacat caccacctcg ttaaacggcc tagcccaaag
    14461 gctgagcggc atggatgatg aattcataat cgatgccgat ctgatcggct ttcatgaggg
    14521 aaagatgtgc tctcaatcgg atatgctcag gtatatcaac cgccgccgcc tctcccgaag
    14581 aagcagcata tcgcctgccc tgctagccta cgatcttatc tacatttctg gcgaggacac
    14641 catctcccta cccttccagg agaggcgaaa gaggatgctg gaagctttgg gcgagcccag
    14701 gtccatgccc ttccagggaa tatctgcgac ccgtgaatgg atcgtcctgg agaaggatct
    14761 aaagaaggac tctattcaga gccatctgga ccggatggtg gaagagggtg gagtgggctt
    14821 gatggtgcgg agccttctgg gcctctaccg gcctggagaa tgcagcagat gcgatttctt
    14881 tgtgggaaga gagatgtcca tatcagccgt tat
//
LOCUS       DAOSGP010000004        14807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_3324_length_14807_cov_19.350122, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000004 DAOSGP010000000
VERSION     DAOSGP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 14807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14807
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3324_length_14807_cov_19.350122"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1748)
                     /locus_tag="PLV49_00240"
     CDS             complement(<1..>1748)
                     /locus_tag="PLV49_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG97299.1"
                     /translation="DRTYLMEHESKAILEELGISTTGSVVARTEEEAVEIFKSLASPV
                     ALKVLSPDVVHKSDAGGVKLHLNEENDVRRAYRDIVSSFQGKLVEGISVQKMAPTGIE
                     AIVGVANDPTFGPVLMFGLGGVFVEVLKDVSFRSIPITRSDAQEMIEEIRGYSLLKGS
                     RGHSADIEALKDLLMRISDFVNLEPSIWEMDLNPVFLYPHGCTVVDARIILGEPRASP
                     PAGKEKQDLHDLFYPQSIAVIGASGTPGKLGWNVFTNLVSHKFKGMLYPINPRAEEIH
                     GIKAYPRISAVPEPVDVAIILVSAGMTADVVEECCQCGVRYIVVESAGFAELGPEGKK
                     IELQMKEIADRYGVRLLGPNCSGIINTHCSMVQSIGIVDALSQGNIGLVSQAGVCAAG
                     MLWGLRHIMDFGIIATIGNKLDINETDIMEAVSKDENINVICMYLESVKGGRRFIDQA
                     RQITMKKPIVVLKSGRTDAGKKAVASHTASLAGNDQIYSAVFRQSGIIRARDYEHMFN
                     LSRAISKQPLPDREGVFIITYAGSLGVIAADAIIDNGMRLSELEEPLKKRLMDLLPDY
                     VCGMNPVDYTFSQDAR"
     assembly_gap    1749..1848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2024..2767
                     /locus_tag="PLV49_00245"
     CDS             2024..2767
                     /locus_tag="PLV49_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HOG97300.1"
                     /translation="MEKDIVQVILEKELTVKNPILIEGFPGIGLVGNIASQYIVNDLK
                     MTYIGAMNSKYFPPLAVLLGGVVNMPVRIYEDASKGVVVLTADIPIHPLASYEVGREI
                     VAWAESINAQELICLAGITVMDDQNRVFGAVSRQELLDKIKGKAEIFEIGTITGITGS
                     IMNECRIRNLPAVCLLGETASAEPDPRSAIAAIETINKIYGLGVNTAKLAEQAEEIEV
                     QMAQLAQQMKAAAPEEQQTQLPKEFPMYG"
     gene            2774..3163
                     /locus_tag="PLV49_00250"
     CDS             2774..3163
                     /locus_tag="PLV49_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HOG97301.1"
                     /translation="MIYTSLTGLSRHVIKEILAARVRTVEIRSPNNFFAVCNCQPGER
                     IFITDSSALDVVPGTTGLITTIKALQVITHRTIQSGENYYEESESQAARLQLQLVGVG
                     RVRRIASIEPATPLTLDVDEVRYCGAR"
     gene            3218..4669
                     /locus_tag="PLV49_00255"
     CDS             3218..4669
                     /locus_tag="PLV49_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase-like protein"
                     /protein_id="HOG97302.1"
                     /translation="MKVLLVTGRLASEQVRRSACGADVLVLDVDVAAFITPEMLCRAG
                     PRGYDLILIPGAITADFRGAEMALGTRIRLGPKHAVDLISLLPRLDEVELSTTVPACV
                     LFEAKRRQEAFIQLERQEAWAPGQLTIKGVKIGGSSRMKVLAEVVDATRLRDEDLVER
                     IRYFEEQGADLIDLGASLDATPASVKRALKKAREVTTLPISIDAVRPELICAGIEAGA
                     DMILSLNGENLPLVGSRVAEAGIPAVVIPGPGPVTLEENLNRAKDYAIQTIADPVLDP
                     PLMGLARSMIRYISLREADPDIPLFFGVGNVTELLDGDSQGANLLLSVLAMEVGASLL
                     FTPEYSAKAKGSIHELAVASRMMVLAKGRQTPPKDLGLDLLILKEKRRIPEEVPPQEL
                     VEASADHKFEADPAGSFRIFLSEGRIMAGNGRITVAGTSARKMLNTLIDRGLIGRLDH
                     AGYLGRELERAEIALRLGRSYIQDEPLWPIEKG"
     gene            4698..5555
                     /locus_tag="PLV49_00260"
     CDS             4698..5555
                     /locus_tag="PLV49_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97303.1"
                     /translation="MNQKAFAIFVGALMVLSGIAYFLPLGTNQKEIAVTSDANSPETF
                     GVKGSLVDWSFDGLRDVLEMAPQNTDMAYWINISSSQNLTDAASIALPQSIGLVYGDQ
                     LYSTRIERLGEVRFNNTWAEFHWIEPYPMGYNSLVIPYEGYMMIPMGTDFVMAMGRPT
                     LFGPQEAVQQSIDVISGGQPSENFTLVDDSGADLQVAALGRGGASMPMAGGYKEFNLR
                     ITSSRSGEDGFDIVARYIQPTADTSRKASDLAEKSDLEYSSSGSEGELSGFVSEENIQ
                     FVLMGLLGP"
     gene            5669..6037
                     /gene="rpl7ae"
                     /locus_tag="PLV49_00265"
     CDS             5669..6037
                     /gene="rpl7ae"
                     /locus_tag="PLV49_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HOG97304.1"
                     /translation="MARPIYVRFDVPADLATKSLEALELARDTGRIKKGTNEATKAIE
                     RGVARLVIVGEDVQPPEIVAHIPALCEEKKTPYIYVKKQSELGAAAGLGVKSAAAAIV
                     EPGKGKEILDEIVQKIGSLK"
     gene            6048..6260
                     /locus_tag="PLV49_00270"
     CDS             6048..6260
                     /locus_tag="PLV49_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HOG97305.1"
                     /translation="MEDDNGIPAEVIEVIGVTGMHGEAMQIKCRILEGNNKGRIITRN
                     SMGPIRIGDVLMLLETAREAKKLTSR"
     gene            6266..6436
                     /locus_tag="PLV49_00275"
     CDS             6266..6436
                     /locus_tag="PLV49_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HOG97306.1"
                     /translation="MEERKCSFCNKTIEPGTGKLYAKKDGTVFYFCSAKCENNVALGR
                     VTRRIKWARKGA"
     gene            6449..6898
                     /gene="ndk"
                     /locus_tag="PLV49_00280"
     CDS             6449..6898
                     /gene="ndk"
                     /locus_tag="PLV49_00280"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOG97307.1"
                     /translation="MERTFVMVKPDGVQRGLVGKILQRIEEKGFKIVAMKLAAMPEAK
                     AKEHYAEHVGKKFYQDLVDFITSGPSVSLVVEGRGAIAAMRKMNGATNPVEAVPGTIR
                     GDWGIETGRNVVHGSDSPASASREIALHFDPSELVTYKRIDEEWLYE"
     gene            6997..8805
                     /gene="infB"
                     /locus_tag="PLV49_00285"
     CDS             6997..8805
                     /gene="infB"
                     /locus_tag="PLV49_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG97308.1"
                     /translation="MQQKSKKAGKAKGKQSNLRTPIVVVMGHVDHGKTTLLDKIRGTA
                     VAKGEAGLITQHIGATEVPLDVVQDFCGSHFGKEIEIPGLLFIDTPGHHAFTSMRSRG
                     GSLADLAVLIVDVNEGFQPQTIESLSILKRFKTPFVVAANKMDRIGGWHTTTNAPFAK
                     SLKVQSERVSEILDTRLYELVGELYKYGFDADRYDRITDFTRTVGIVPISAITGEGVP
                     DLLMILVGLAQRFLKDNLQLTATGPGVGTILEVKEEKGLGTTLDVILYNGEFFAGDTV
                     IVGTSREPLVTKIRALLKPKPLAEIRSEERFLPVKHVSAASGVKVSAPKLDNALAGST
                     IRVVSNPDEVEALSLELKSELDAVRIDTESEGLIIKSDTIGSLEALVGELKARDIHIH
                     YADVGPISRRDVIRAAAINDPLLSAILGFNVDILPDALNEIQKTNVPVFSSDIIYTII
                     ESYERWVEDQKNKMEQERLEAVIRPGAVRILPDCVFRQSKPAVVGVQVIGGIVRTQVN
                     LMREDGANVGVIKGIQAHNENQGSATVGQEVAVSIDGPTVGRQIHEGDILYVNIPEKH
                     ARIVELELKPKLAEDEREVLENFLEIKRKKDPFWGR"
     gene            8849..9448
                     /locus_tag="PLV49_00290"
     CDS             8849..9448
                     /locus_tag="PLV49_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOG97309.1"
                     /translation="MAMPLNEIIIPLIAIGLAEMGDKTQLSVILLSSRTREYIPLLAG
                     VMLAFLITDGFAILIGSWMTGIIPLDLLKLISGGVFILFGALILRGDQKEAEEERELS
                     SGNALLSGFSLIFLSEWGDKTQIASALFATEYNPIMVFIGVMAALFILSVMAIYLGQI
                     ISQKVDRKLVSRIAGTIFLIIGIAIILSLLAPYTFAIIS"
     gene            complement(9535..9729)
                     /locus_tag="PLV49_00295"
     CDS             complement(9535..9729)
                     /locus_tag="PLV49_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOG97310.1"
                     /translation="MVDGTVKFFNRVKNFGFIAGDDGKDYFVHVTGLKPGVAIDEGDK
                     VSFDVIEGEKGPKADKVEKV"
     gene            9905..11575
                     /gene="thsA"
                     /locus_tag="PLV49_00300"
     CDS             9905..11575
                     /gene="thsA"
                     /locus_tag="PLV49_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HOG97311.1"
                     /translation="MAGMGGTPVIILRDTRRESGNEAISNNIMAARAVANAVRSSMGP
                     KGMDKLLVDSIGDVTITNDGVTILKEMDVQHPAAKMLIEAAKTQDKEVGDGTTTVAVL
                     AGELLRKAEVLLDQKVHPTMIVQGYRMAADEAVKIAKSIAFEAQESDRVLLERIARTA
                     MTGKLADSPDSKMAGYAVDLVLNATEDYGDKRVFDMDRVNVEKKVGESTADSEIIEGV
                     VIDKEIVHQNMPRKVTDAKVALLSCPIESKDTETKTEVQITSSDQFQMFMEHEKKRVK
                     EAADKVIASGANVVFCQKGIDDLAQHYLAQAGVLALRRVIKKDLDKLAKATGANIVTS
                     LDELKSSDLGSAALVEERRVGSGIMTFITGAKNSAATLLLRGGTQQVLDGLERALDDA
                     LHAVADVVEDKKLVVGGAAPEIEIALRLREYAATLKGREQLAVAKFAEAMEIVPKTLA
                     ENAGFDAIDKVVELKSRHSSNKNIGLNAYTGETVDMYELGVVEPLRVKIQAILSATDA
                     ACLILRIDDVLASTKEPPARGPGGMPPGMGGMGGMPGMGMGGMPPGMF"
     assembly_gap    11710..11809
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11810..12367
                     /locus_tag="PLV49_00305"
     CDS             <11810..12367
                     /locus_tag="PLV49_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOG97312.1"
                     /translation="EEELKKTKRSAEYNSSERLQYLESYLARQEQNPSAIFLGTFPPL
                     RLHSIEDRLCRMLETARFEVVVVSPWIKRQTWNRIAAPLQKFFRRGGRLVVFMRGRES
                     DYSSGMSDDIQDEVRRLGGELLFVRPLHAKIYMIDRKEAIVTSANLSQGGIDDNYEAG
                     VWLNDPQVIHDICSYVDGLYRCRQN"
     gene            12409..12972
                     /locus_tag="PLV49_00310"
     CDS             12409..12972
                     /locus_tag="PLV49_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG97313.1"
                     /translation="MCSGFGSCEVYHPDEDTFLLLQAAIKEARPKDHVLEVGCGSGLV
                     SLELVRQVESLVALDINPHAVRATRERGVEVIRSDLFGGIRGKFDLIIFNPPYLPTER
                     AERSDQWINYALDGGESGRETIGRFLLNLAGYLWPGGRALLLISSLTGPEEVEMMAGD
                     AGLITERVASKGCFFEKLMVLRITVRN"
     gene            complement(12956..13903)
                     /locus_tag="PLV49_00315"
     CDS             complement(12956..13903)
                     /locus_tag="PLV49_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOG1 family protein"
                     /protein_id="HOG97314.1"
                     /translation="MFERLPTIPTSQELLDKSFRRASRAADNESMVLNAGNILSDNLA
                     NLIRKFPSFENLPPFYREMVEITVGVDKIRISLSRLRWASRQTRLITREYVGKIRRAR
                     GTDSTPLRKSAFGRFSSVMRSIEKDMLFLNSARNLLRKMPTIDPEVPTILIAGYPNVG
                     KSSFIIRITGAKPEVASYPFTTRGIIVGHFVRDDKRYQVVDTPGLLDRPMSERNEIER
                     QTVAALSHLQGVVLYILDPSEHCGYPLDSQLRLAEDLSNWIKLPMLIVANKVDIKRYS
                     DLAEMSTETGEGVSLVLERLIALLDSKGDGLTGELNCGL"
     gene            14057..14341
                     /locus_tag="PLV49_00320"
     CDS             14057..14341
                     /locus_tag="PLV49_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HOG97315.1"
                     /translation="MKRLGTALHVVQNKLIVQSEEIVGSETAIPRGNSWVVDQKRTRV
                     GKVFDIFGPIDRPYIIVRPNRGTDAAAHIGKKLYIDEAPGRDSKWKKLKS"
     gene            14320..>14807
                     /locus_tag="PLV49_00325"
     CDS             14320..>14807
                     /locus_tag="PLV49_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HOG97316.1"
                     /translation="MEEIEKLREIERAEPEKLKERTRLRREKEKVDEFEKFTVECPEC
                     KSHTLVRDYERAELVCSDCGLVIDENFIDQGPEWRAFDHDQRMKRSRVGAPMTYTIHD
                     KGLSTMIDWRNRDSYGKTISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELDRMA
                     SAL"
BASE COUNT         3553 a         3748 c         4199 g         3107 t
ORIGIN      
        1 tcctggcatc ctggctgaag gtgtagtcca ccgggttcat gccgcagaca tagtccggca
       61 aaaggtccat cagcctcttc ttcagaggct cctccagctc ggagaggcgc atgccattgt
      121 cgatgatggc atcggcagct atcaccccca acgagccggc ataggttatg atgaaaaccc
      181 cctcccggtc aggcaggggc tgcttggata tagctctgga aaggttgaac atatgctcat
      241 agtcccgagc gcggatgatc cccgactggc ggaagacggc gctatagatc tggtcgtttc
      301 cagccaggga agcagtatgg gaggccacgg ccttctttcc cgcatcggtc cggccggact
      361 tgagcaccac tatgggcttt ttcatggtga tctggcgagc ctggtctatg aacctcctgc
      421 cgcctttcac cgactccaga tacatgcaga tgacattgat gttctcgtcc ttgctcaccg
      481 cctccatgat atcggtctca ttgatgtcca atttgttgcc tatggtggct atgatgccga
      541 agtccatgat atggcgcaga ccccagagca tcccggctgc gcacacccct gcctgggaga
      601 ccaggccgat gttgccctga ctcaaggcat ccacaatacc tatggactgc accatgctgc
      661 agtgagtgtt gatgatcccc gagcagttcg gtccaagcag ccgcacgcca tacctatctg
      721 cgatctcttt catctgcaat tcgatcttct tgccctctgg ccccagctcc gcaaacccgg
      781 ccgactcgac cacgatatag cgaacgccgc actggcagca ctcctccact acatccgcgg
      841 tcatgcctgc agagaccaga atgatggcca catccaccgg ctcgggcacc gcgctgatgc
      901 ggggataggc cttgatgcca tgaatctcct ctgctcgggg gttaatggga tagagcatac
      961 ccttgaactt gtggctgacc agattggtga agacattcca gcccagcttt ccgggcgtac
     1021 ccgaagcccc gatcaccgct atgctctgag gatagaagag gtcatgcaga tcctgcttct
     1081 ctttcccggc gggtggactg gccctgggct cgcccaggat gattctggca tcgaccacag
     1141 tgcaaccatg aggatagagg aacacgggat tcagatccat ctcccagatg ctgggctcca
     1201 agttcacaaa atccgatatc ctcatcagta gatccttcaa ggcctcgata tccgcagagt
     1261 ggccccggct gcccttgagc agggaatacc cccggatctc ctcgatcatc tcctgtgcat
     1321 ctgagcgggt gatggggatg gagcggaagc tgacatcctt caagacctca acgaataccc
     1381 cccccaggcc gaacatcaga accggcccga aggtggggtc gtttgccact cccacgatgg
     1441 cctctattcc cgtaggggcc atcttctgca cagagatacc ctccaccagc tttccctgaa
     1501 atgagctgac aatatcccgg taggctcgcc ggacgtcgtt ctcctcattc aaatggagct
     1561 tgaccccacc tgcatcggat ttatggacta catcaggaga gagaaccttc aaggccactg
     1621 gactggccag agacttgaat atctccactg cctcctcttc ggttcttgct accacggagc
     1681 cggttgtgga tatccccagc tcctccaaaa tggccttgct ctcatgctcc atgaggtaag
     1741 ttctgtctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ccagctcctc
     1861 caaaatggcc ttgctctcat gctccatgag gtaagttctg tctactgatt ttgcctgatc
     1921 gataatgtct ttgattactt ctgccatttt caaccccaga ttgctctttt tcgttctgct
     1981 ctgaagcgat gatcttatct tagataaaag gttattatct ttcatggaaa aggatattgt
     2041 ccaggtcatc cttgaaaagg agctcactgt caaaaaccct attctgatag agggattccc
     2101 gggaatcggc ctggtaggta acatcgccag ccagtacata gtcaacgatc tgaagatgac
     2161 ctatatcgga gcgatgaact ccaaatactt cccgccactg gcagtccttt tgggaggagt
     2221 agtgaacatg ccggttcgca tctatgagga tgcgagcaag ggggtagtgg ttctgaccgc
     2281 cgatattccc atccatcccc tggcctccta tgaggttgga agggagatag tggcctgggc
     2341 agagtccatc aatgcacagg agttgatctg cctggcagga ataaccgtga tggatgatca
     2401 aaaccgggtc tttggcgctg taagccgcca ggagctgctg gataagataa agggcaaagc
     2461 agagatcttt gagataggaa caatcaccgg aattaccggt agcatcatga atgagtgccg
     2521 cataagaaac ctccctgcgg tctgcctact gggggagacg gcatcagcag aacctgatcc
     2581 ccgctcggcg atcgctgcca tcgagaccat taacaagatc tatggcctgg gagtaaacac
     2641 tgccaagctc gctgaacagg cagaggagat cgaggtgcag atggctcaac tggcccagca
     2701 gatgaaagcg gccgcacccg aggaacagca gacccagttg ccgaaagagt tcccaatgta
     2761 cgggtgatgc cagatgattt acacaagcct aaccggatta tccagacatg tgataaaaga
     2821 gattctcgcc gccagagtga ggacggtgga gatcaggagc ccgaacaatt tctttgccgt
     2881 ctgcaactgc cagcctggcg agaggatctt catcaccgat tcaagcgccc tggatgtagt
     2941 tccaggcaca accggcctta tcaccaccat aaaggccctt caggtcataa cccacagaac
     3001 catccagtct ggagagaatt attatgagga gagcgagtct caggcagcaa ggctgcagct
     3061 tcagctggtg ggcgtcggtc gggtgagaag gattgcgtcc attgagccag caacgcccct
     3121 cactttagac gtggatgagg tgaggtactg cggggcacgt taaacgcccc cttttttcca
     3181 aagtagaacg cctgagaaga gtggcattgc ccaagcgatg aaggttcttc tcgtcaccgg
     3241 aaggctggcc tctgagcagg tcagaagatc agcctgtggc gcggatgtgc tagtgctgga
     3301 tgtcgatgta gcggcgttta tcaccccgga gatgctctgc cgggcagggc ctcgaggata
     3361 cgacctgatt ctgatcccag gagccataac cgccgatttc cgaggggcgg agatggctct
     3421 tggcaccagg atccgcctag ggccgaagca tgctgtagac ctgatatcgc tcttgccccg
     3481 cctggatgag gttgagctct ccactactgt tccggcctgc gtcctatttg aggcgaagag
     3541 aaggcaggag gccttcattc agctggagcg gcaggaggca tgggccccgg ggcagttgac
     3601 catcaaaggc gtcaagatcg gcggcagctc gcggatgaag gtcctggcgg aggtagtcga
     3661 tgccaccagg ctccgggatg aggatctggt ggagcggatc agatacttcg aggagcaggg
     3721 tgccgatctg atcgacctgg gcgcatcctt ggacgccacc cccgccagcg tcaaaagagc
     3781 gctgaagaag gcaagggaag taactaccct tcccataagc atagatgccg ttcggccgga
     3841 gctcatctgt gccggcatcg aggccggggc ggacatgata ctcagcctga acggcgaaaa
     3901 cctgcccctg gtggggagca gggtggcaga agcgggcata ccggcagtgg tcataccggg
     3961 acccggaccc gtcaccctgg aggagaacct gaacagagca aaggattatg caatccagac
     4021 tattgccgat ccggtcctcg atcccccctt gatgggcctg gcccggtcga tgatcagata
     4081 catcagcctc cgtgaggccg atcctgatat ccctcttttc ttcggggtgg gcaatgttac
     4141 tgagctcttg gatggagaca gccagggggc caacctgctc ctatccgtcc tggcaatgga
     4201 agtgggggcc tcattgctct tcaccccaga atacagcgcc aaggcaaagg gctccattca
     4261 cgagctggct gtggcctcga ggatgatggt tctggccaaa gggcgccaga cccctccaaa
     4321 agacctgggc ctggacctat tgatactcaa agagaagagg agaattccgg aggaggttcc
     4381 tccccaggag ctggtggagg cctcggcaga ccataaattc gaggctgacc ctgccggaag
     4441 cttcaggatc ttcctctccg aagggaggat catggctgga aacggcagga tcacagtagc
     4501 tggcacgagc gcccggaaga tgcttaacac cctcatcgac cggggattga taggaaggct
     4561 ggaccatgcc ggctacctgg gcagggaact ggagcgtgcg gagatagccc tgcgcctggg
     4621 ccggagttat atacaagacg agcccctatg gccgatagag aaaggttaaa taataattat
     4681 ttcgtgaggg agagaggatg aatcagaagg ctttcgctat cttcgtaggc gctcttatgg
     4741 tgctctcagg aattgcatat ttcctgcccc tgggcaccaa ccagaaagag attgctgtta
     4801 catcagatgc caattcccct gagacctttg gcgttaaggg gagcctggtg gactggagct
     4861 tcgatggact gagagacgtg ctggagatgg caccgcagaa cactgatatg gcatactgga
     4921 taaatatcag ttcatctcag aatctgaccg atgcggcaag tatcgccctc ccgcagtcca
     4981 taggcctggt ctatggggac cagctttatt ccaccaggat cgaaaggctg ggagaggttc
     5041 gcttcaataa cacctgggcg gagttccact ggattgagcc ctatccaatg ggctacaaca
     5101 gcctggtcat tccctatgaa ggctatatga tgatccccat gggcaccgac tttgtcatgg
     5161 ccatgggcag gccaactcta ttcgggccgc aagaagcagt acagcagtcc atcgatgtca
     5221 tatccggtgg ccagccatcg gagaacttca ccctggttga tgacagcgga gccgacctgc
     5281 aggtagcagc cctcggtcgc ggtggagcga gcatgcccat ggctggcggg tataaggagt
     5341 tcaacctgag gatcacatcg tccaggagcg gtgaggatgg cttcgacatc gtcgcccggt
     5401 atatccagcc aacagcagat actagccgga aagcgagcga tctagctgaa aagagcgacc
     5461 tagagtattc cagcagtgga tcagaaggag agttatccgg atttgtttcc gaggaaaaca
     5521 ttcagtttgt gctgatgggg cttcttgggc cctagcgcaa cctttttaca tgtggaacta
     5581 atggcagatc tagtcttgag gtataatcca aaaggcgctt ttgcgatgaa tgatgatggg
     5641 cgttgtcgcc cggaaggaga attaagttat ggcaagacca atatatgtaa ggtttgatgt
     5701 tccagcagat ctggccacaa aatcccttga ggccttagag ctggctcgcg ataccggccg
     5761 aataaagaag ggcacaaacg aggccaccaa ggccatagag aggggtgtag cccgtctggt
     5821 aatagtaggc gaggatgttc agccccctga gatcgtcgct catattcccg ctctatgcga
     5881 agagaagaag accccctata tctacgtcaa gaagcagagc gagcttggtg ccgctgcagg
     5941 ccttggcgtt aagagcgcag ccgcagcgat cgtggagccg gggaaaggaa aagagattct
     6001 ggatgagatc gtccaaaaaa ttggatctct gaagtaggtg atatcctatg gaggacgata
     6061 acggaatacc ggcagaggta atcgaggtca tcggcgtcac cggcatgcat ggggaagcaa
     6121 tgcagatcaa gtgccgtatt cttgagggca ataacaaggg gagaatcata accaggaaca
     6181 gcatgggacc catacgaatc ggcgatgtcc ttatgctgct ggagacggca agagaggcaa
     6241 agaagcttac cagcaggtga ggaagatgga agaaagaaag tgcagcttct gcaataagac
     6301 cattgagcct ggcacgggca agctctatgc caagaaggac ggaacggtat tttacttttg
     6361 ttctgccaaa tgcgagaaca acgttgccct tggccgggta actagaagaa taaagtgggc
     6421 ccgcaagggg gcataaggcc tggcggatat cgagagaaca ttcgtcatgg tcaagcccga
     6481 tggtgtgcag agaggcttgg tgggcaagat cctgcaacgg attgaagaga agggcttcaa
     6541 gatcgtggcc atgaagctcg ctgcgatgcc tgaagcgaag gctaaagagc attacgcaga
     6601 gcatgtgggc aagaagttct accaggatct ggtggacttc atcacctcag gaccatccgt
     6661 ctccctggtg gtcgagggaa ggggtgccat tgccgccatg agaaagatga acggcgccac
     6721 aaatccagtg gaggccgtac cgggaaccat tcgcggtgac tggggcattg agacgggacg
     6781 gaatgtggtg cacggctcag actccccggc gtcagcatct cgcgagatcg ctcttcactt
     6841 tgatccctca gagctggtga catacaagcg aatagatgaa gaatggctct atgagtagct
     6901 cctggcagat gcttctgcca agccctcatg ggagccttta aattcccaaa aaataaatca
     6961 cttttttagg gctaattctg tgagaagcga tatttcatgc agcagaaatc caaaaaggcc
     7021 ggcaaggcaa agggcaagca atcaaacctc aggacaccga tagtagtggt gatggggcat
     7081 gtggaccatg gcaagaccac actcctggat aagatccgag gtaccgcagt agccaaggga
     7141 gaggccggcc tgataaccca gcacattgga gccacagagg ttcccttgga tgtggttcag
     7201 gatttctgcg gatcgcattt cggaaaggag atcgagatac cgggactgct gttcattgat
     7261 acgccgggcc accatgcatt cacatccatg cgcagccggg gcggctccct ggccgatctg
     7321 gcagtgctga tcgtggatgt aaacgagggc ttccagcccc agaccatcga atccctgagc
     7381 attctcaagc gcttcaagac cccatttgtg gtcgctgcca acaagatgga ccgcattggc
     7441 ggatggcata ccaccaccaa tgctcctttt gccaagagcc tgaaggtgca gagcgagagg
     7501 gtctcagaga tcctcgatac caggctctat gagcttgtgg gtgagctcta caagtatggc
     7561 tttgacgcag accgctatga tcgcatcacc gatttcaccc ggacggtggg catcgtgccc
     7621 atcagtgcca taaccggaga aggggttcct gaccttctca tgatcctggt gggcctggcg
     7681 cagagattcc tcaaggataa cctgcagctc accgccaccg gtccaggggt gggcaccatt
     7741 ctcgaggtca aggaggagaa ggggttgggc actacattgg atgtcatact ctacaatggg
     7801 gagttctttg ccggggatac tgtgatcgtc ggcacctcgc gcgagcccct ggtcaccaag
     7861 atcagggctt tgctcaagcc caaaccgttg gctgagatca ggagcgagga gaggttcctt
     7921 ccggtaaagc atgtatctgc cgcttctggg gtgaaggtct ccgctccaaa gctggataac
     7981 gccctggccg gatcaaccat ccgggtggtc tccaatcccg acgaggtaga ggccctctcc
     8041 ctggaattga agtccgagtt ggacgcagtc aggatcgaca ccgagagcga ggggttaatc
     8101 atcaagtcgg acaccatcgg ctcgctggag gccctggtgg gggagcttaa ggcaagggat
     8161 attcacattc actatgcaga tgtggggccc atatccagaa gggatgtgat aagggccgct
     8221 gccatcaacg atccccttct ttccgccatc ctgggattca atgtggatat actgcccgat
     8281 gccttgaacg agatccagaa gacgaatgta cctgtcttct ccagcgatat catctacacc
     8341 atcatcgaga gctatgaacg ctgggtggaa gatcagaaga ataagatgga gcaagagcgc
     8401 cttgaagcgg tcatccgacc gggggcagtg cgcatacttc cagattgtgt cttccggcaa
     8461 tctaagcccg ctgtggtggg agttcaggtg atcggcggga tcgtcaggac ccaggtcaat
     8521 ctaatgagag aggacggggc caatgtagga gtgatcaagg gaatccaggc ccacaatgag
     8581 aaccagggat cggcaaccgt cggccaggag gtggccgtat ccatcgacgg gcccactgtg
     8641 ggccggcaga tccatgaagg tgatatcctc tatgtcaata tcccggaaaa gcatgcccgc
     8701 attgtggagc tggagctgaa gccgaagctg gccgaggatg agagagaggt gctggagaat
     8761 ttcctggaga tcaaacgaaa gaaggaccca ttctggggaa ggtaggatat gaccttgcag
     8821 cgaactgagt tcaggcggga tgacgccgat ggccatgccg cttaacgaga tcataattcc
     8881 cctcatcgcc atcggcctgg cggagatggg cgacaaaact cagctctctg tgatactcct
     8941 ctcctcccgc acccgggagt acatccccct cctggccgga gtcatgcttg cttttctgat
     9001 cacagatgga tttgccattc tcataggctc ctggatgaca ggcataattc ccctcgatct
     9061 gctaaaactg atatctggcg gggtgttcat tctgttcgga gcattgattc tcagaggaga
     9121 tcaaaaggag gcagaagaag agagggagct atcgtccgga aacgctcttc tatccggatt
     9181 ttcccttatc tttctatcgg agtggggaga caagacccag atcgcatccg ccctctttgc
     9241 cacggagtat aatccaatca tggtcttcat aggggttatg gcagcgctat tcatcctctc
     9301 cgttatggca atctatctgg gacaaatcat ctctcagaag gtcgacagga agctggtatc
     9361 caggatagcg ggcaccatat tcttgataat aggcatagct atcatcctct ctctgttggc
     9421 gccatatacg tttgctatta tatcataatg gcccaataaa atagattata gcaataggaa
     9481 gggcctttgc cctgctatcg ctttgctgaa tttatgtagt ggctatatta ttatctaaac
     9541 cttttctacc ttatcggcct tggggccttt ctcgccctcg atgacatcga agctcacctt
     9601 gtcgccctca tcgattgcga caccgggctt caaaccagtc acatgaacga aatagtcctt
     9661 accatcgtca ccagcaatga atccaaaatt cttgactcga ttgaaaaact taactgttcc
     9721 atctaccatt ttttattcct caataagatt agaccttctg gaggagcttc aactacttaa
     9781 agttgctggc agcttccctt caggaggcaa ataacagcca tgtgaagccc aatcgataag
     9841 aaaaggtttt atagaagagc aaatactgct taaccgagaa tcatttgctg gaggattatt
     9901 atacatggca ggaatgggtg gaacaccagt tatcatttta agagataccc gcagagaatc
     9961 gggaaacgaa gccatttcga ataatattat ggccgcccgc gcagtggcca acgccgtgcg
    10021 cagctcaatg ggccccaaag gcatggacaa gctgctggtg gattccattg gcgacgtcac
    10081 catcaccaac gatggggtga ccatcctcaa ggaaatggat gtgcagcatc ccgctgccaa
    10141 gatgctcatc gaggccgcca agacccagga taaagaggtg ggtgacggca ccaccactgt
    10201 agctgtgctg gcgggggagc ttctcaggaa agcggaggtg ctcttagacc agaaggtcca
    10261 ccccactatg atcgtgcagg gctatagaat ggcagctgat gaagcagtaa aaattgcaaa
    10321 gtcaatcgcc ttcgaggccc aggagtcaga tcgggtgctg ctggagagaa tcgcccggac
    10381 ggctatgacc ggcaagctgg cagattctcc tgacagcaag atggccggct atgccgttga
    10441 tctggtcctc aatgccacag aggactatgg ggataagagg gtcttcgaca tggacagggt
    10501 caacgtggag aagaaggtgg gcgagagcac agcagactca gagatcatcg agggagtggt
    10561 gatcgataag gagatcgtcc accagaacat gccccgcaag gtcacagatg ccaaggtggc
    10621 cctgctctcc tgtcccatcg agtccaagga cactgagact aagactgagg tccagatcac
    10681 ctcctccgat cagttccaga tgttcatgga gcatgagaag aagagggtaa aggaggcggc
    10741 agataaggtg atcgccagcg gcgcaaatgt ggtcttctgc cagaagggca tcgacgacct
    10801 ggcccagcat taccttgcac aagcgggggt cctcgccctg agaagggtga tcaagaagga
    10861 cctggacaag cttgccaagg ccacgggagc taacatcgtc accagcctgg atgaattgaa
    10921 atcctcggac ctcggatcag ccgctctggt ggaggagcgg agggtgggaa gtgggatcat
    10981 gaccttcatc accggcgcca agaatagcgc tgcaacccta ctcctccggg gcggaacgca
    11041 gcaagtcctg gatggcctgg agagggccct tgacgatgcc ttacatgcgg tggcagacgt
    11101 ggttgaggac aagaagctgg tggtgggcgg tgcagccccg gagatcgaga tcgcattgcg
    11161 tctgcgcgaa tatgccgcca ctctgaaggg gagggagcag cttgccgtcg ccaagttcgc
    11221 tgaggcgatg gagatcgtgc ccaagactct ggccgagaac gctggctttg atgccatcga
    11281 taaggtggtt gagctgaaga gcaggcactc cagcaacaag aacatagggc tcaatgccta
    11341 taccggggag acggtggata tgtatgagct gggcgtggtc gagcccctgc gggttaagat
    11401 tcaggctatt ttgtcagcca ctgatgctgc ttgcttgata ctgcgcatcg atgatgtctt
    11461 agcctccacc aaggaacctc ctgcacgcgg tccaggcggc atgcctcctg ggatgggggg
    11521 catgggagga atgcccggaa tgggaatggg aggaatgcct cccgggatgt tctgatcatc
    11581 ccgaaaccat ttttttaggc acagacatac tcttttggtt tattttggtt tgttgaggat
    11641 gccgaatgga agggtattgg atctggctgg catttctggc tttagcggtg atatccctca
    11701 gcatcatccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aagaggagct
    11821 caagaagacg aaaagatctg cagaatacaa ttcgtcggaa aggctgcagt acctggagag
    11881 ctaccttgcc cgccaggagc aaaatccttc agccatcttc ctgggcacct ttccacccct
    11941 tcgcctccac tccattgagg accgactctg caggatgctg gagacggcca ggttcgaggt
    12001 ggtggttgta tccccctgga taaagaggca gacctggaat cggattgctg ctccattgca
    12061 gaagttcttc cgccggggag ggaggctggt ggtgttcatg agagggcggg aatcggacta
    12121 cagctcagga atgagcgatg acatccagga tgaggtaaga aggttgggag gagagctcct
    12181 tttcgtccgg ccgctccatg ccaagatcta tatgatcgac agaaaagagg cgatagtgac
    12241 ctctgccaat ctctcccagg gagggatcga tgacaactac gaggcagggg tatggttgaa
    12301 cgatccccag gtcatccatg atatctgctc ttacgtggat ggcctgtaca ggtgccggca
    12361 gaattgaaaa aggaaaggat tatttaggcg agttggagga ttgagataat ttgttctgga
    12421 ttcgggtctt gtgaggttta tcatccggac gaggatacat ttctcctctt gcaggcggcc
    12481 ataaaagagg cccgcccaaa ggatcatgtg ctcgaggtag gctgcggttc gggtctcgta
    12541 tccctggaat tggtcagaca ggtggagagc cttgtcgccc tggacataaa tcctcatgcc
    12601 gtcagggcga ccagggagag aggggtggag gtgataagat ccgacctctt cggaggaata
    12661 agagggaaat tcgacctgat catcttcaat cccccttacc ttcccaccga acgggcagag
    12721 agaagcgatc aatggatcaa ctatgccctg gacggagggg agagcggcag ggagaccata
    12781 ggccggttcc tgctgaatct ggctggctat ctttggccgg ggggaagagc actgctgctc
    12841 atatccagcc tgaccggacc ggaagaggtg gagatgatgg caggggacgc cgggcttatc
    12901 acagagcggg tggccagcaa aggatgcttt tttgaaaagc tcatggtgct gagaatcaca
    12961 gtccgcaatt gagttctcct gtaagtccat cgcctttact atccaggaga gcgatcagcc
    13021 tctccagcac tagagagacg ccttctccgg tctcggtgga catctctgcc agatcactgt
    13081 atctcttgat atcaacctta ttggcaacga tcagcatggg cagcttgatc caattggata
    13141 gatcctcggc cagacgaagc tgtgaatcca gaggatagcc gcagtgctcg cttgggtcca
    13201 gaatgtatag cactaccccc tgcagatggc tgagggcagc cacggtctgc ctctcgatct
    13261 catttcgctc cgacattggc cggtcgagca atccgggagt gtccaccacc tgatacctct
    13321 tatcgtcacg aacgaagtgg cccacaatta tcccccgagt ggtaaaagga tagcttgcca
    13381 cctctggttt ggccccagtg atgcggatga tgaagcttga tttccccaca ttgggatagc
    13441 cggcgatgag aatggttgga acttccggat ctatggtcgg catcttgcga agcagattcc
    13501 gggcagaatt caggaacagc atatcctttt caatcgatct catcaccgat gagaagcgcc
    13561 caaaggccga ctttcggagg ggagtgctgt ccgtccccct ggcccgccgg atctttccca
    13621 catactctct ggttatcagt cttgtctgcc tggaggccca gcgcaggcga gataaactga
    13681 tacggatctt atctacgcca accgttatct ccaccatctc cctgtaaaaa ggaggtaggt
    13741 tctcaaagga gggaaacttc cggatcaaat tggcaaggtt atctgagagg atattgcctg
    13801 cattcaggac catggactca ttgtcggcag cacgggaggc gcgccggaat gatttatcta
    13861 aaagctcctg cgatgtgggg atagttggaa gacgctcaaa catcagtgag gacttgccgg
    13921 cattaacaga taaagcttcg gtccggcctt ctgtctcctt tggggcatgc gcggcgtgga
    13981 ttgcgggatt catttaataa atggttttcg gaaggcttaa atagtacttc tttatctata
    14041 tagatgaggt gaccttttga agaggctagg caccgcgctt catgtcgtgc agaataaact
    14101 gatcgtccag agtgaagaga tcgttggaag cgagaccgct atccccaggg ggaactcctg
    14161 ggtggtggat cagaaaagga ccagggtcgg aaaggttttc gatatattcg gtccaatcga
    14221 tcgtccatat ataatcgttc ggccgaatag aggaacagat gccgctgccc acatcggaaa
    14281 gaagctctat atcgatgagg cgccggggag agatagcaag tggaagaaat tgaaaagctg
    14341 agagagatag agagggcgga gccggagaag ctcaaggagc gtacccggct cagacgcgag
    14401 aaagagaagg tagacgagtt tgagaagttt acagtcgagt gtccagagtg caagagccac
    14461 accctggtgc gcgactatga acgagcagaa ctggtttgct ccgactgcgg cctggtgatc
    14521 gatgagaact tcatagatca ggggccggaa tggagagcct tcgaccacga tcaacgcatg
    14581 aagaggtcga gggttggggc gcccatgacg tacaccatcc acgacaaggg actctccacc
    14641 atgatcgact ggaggaacag ggactcatat ggcaagacca tatcctccaa gaatagggct
    14701 cagctctacc gcttgagaaa atggcagagg cgcatccggg tcagcaatgc cacagagcga
    14761 aacctagcct ttgccctgtc cgagctggac cgaatggcat cagctct
//
LOCUS       DAOSGP010000005        14273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_3515_length_14273_cov_16.418484, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000005 DAOSGP010000000
VERSION     DAOSGP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 14273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14273
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3515_length_14273_cov_16.418484"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..105)
                     /locus_tag="PLV49_00330"
     tRNA            complement(join(4..39,68..105))
                     /locus_tag="PLV49_00330"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Met,seq:cat)
     gene            207..1403
                     /gene="coaBC"
                     /locus_tag="PLV49_00335"
     CDS             207..1403
                     /gene="coaBC"
                     /locus_tag="PLV49_00335"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOG97317.1"
                     /translation="MPSDISASVSETLSGKTIVVGVTGSIAAVRVIDLIRDLIRSGAE
                     VHCTMSHAAGQILHPYALEYASNHPVITEITGRVEHVQFCGVGGKADLFLVAPATANT
                     IGKMANGIDDTPVTTFATTALGSGKPVAVVPAMHEAMYRHPAVIRNLQILREMGVALI
                     DPRIEEGKAKFADNQRVVQEVERLLGPADLKGKRILITSGSNAESIDPIRILTNRASG
                     KTGVALALEARRRGADVTLVHRFIQDLPVHQVYAQSASDMLDAVMAELKKGCDALISA
                     AAVADYTLDPSEEKIKSGLELNLRLKPTRKIIKAVREAYPDLKIVGFKAETDTGDEEL
                     LARARASLEGAGLDLVVANDVSRGGMGTDDNRVLILSRSGSHSEVEGKKSLIARRIID
                     SLAEIL"
     gene            1424..>1812
                     /locus_tag="PLV49_00340"
     CDS             1424..>1812
                     /locus_tag="PLV49_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOG97318.1"
                     /translation="MTTRAWVPSHITGFFAARREEDPLQSGSIGCGLCLSPGATTIIE
                     SSPDIEDSKIILNGRVTDAPVSRFVIERLARSPVRVKTDMNMPFGSGFGASGAGALGC
                     AYALNHHFDLGLTADQTASVAHAAEVV"
     assembly_gap    1813..1912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1913..2424
                     /locus_tag="PLV49_00345"
     CDS             <1913..2424
                     /locus_tag="PLV49_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOG97319.1"
                     /translation="GLTADQTASVAHAAEVVNRTGLGDVIAQNTGGLVIRLEPGAPGI
                     GRIDRIPVAPQPVHCVVRGPISTREVLSDQGTMSSINEAGRAALKDLLKKPTLEEFMR
                     LSRRFTVQTGLASSWALDMIEAVEAEGGRASMIMLGDAVYAFGGIEALQGSGQVYTTF
                     ISQRGANLD"
     gene            2408..3181
                     /locus_tag="PLV49_00350"
     CDS             2408..3181
                     /locus_tag="PLV49_00350"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HOG97320.1"
                     /translation="MLILTEIPKSHPRYASLVARERVAEGVKKGYTSTQGLIAQGRGE
                     CFDYLLGERTTASAAAAIRAGAALLLLAERPAISVNGNVAALVSEEMVALARALNIPL
                     EVNLFHRSEDRVKKIADLLREKGAEDVLGERPDTSVPGLDHARALATRGGIYDADVVL
                     IPLEDGDRCEALLAMGKRVIAIDLNPLSRTAKKATVSIVDNILRAVPQLTEEVRQLSN
                     KPRPDLEKILNEYDNQETLAGSVQEIRDHLDEQFRKGSD"
     gene            complement(3178..5901)
                     /locus_tag="PLV49_00355"
     CDS             complement(3178..5901)
                     /locus_tag="PLV49_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOG97321.1"
                     /translation="MDTIIEAHSIPAYDAIEALKANPEGGLGKDEVAERLRIYGPNSL
                     PKPKPTSIAVYFLRQFYSPLIYILLLAAAISLLLGDWQESIFIMIVLLLNSIIGTIQE
                     YSAEKSAEALKNMVRTKTRVIRDGEEFEIDSEELVPGDIVLLESGGKVPADIRLIEVQ
                     DLVVDESLLTGESIPDAKDPDLILDRRTPLGDRENMAFAGTIVVSGRGRGVVVATASN
                     TAVGGLAASLAGKPETKPPLILRMEKFNSKIALGVAVAVAVIFAVEIYRGEPIYDIFL
                     QSVALAVAAVPEGLPVAMTVALSIGMSRMAKRNVIVRRLVAVEALGSCNFIASDKTGT
                     LTVNQLTVTRAVIPGRDSWNVIDLAKSPREVAEEAAGKMAAEGPVGSQSAGQTSALKA
                     LARASVLANEAFSGFRDGSWDYHGDAVDVALLMMAQETGVTQQEEQSNYPRIAQIPFE
                     SKRQFSATLHQGPDGRIAFAKGSPEKILKMSSRMATPDGEIPLDPQGADKVAEALATA
                     GFRLLALASGRFPENQEFAEDRLEGLTFLGLVGMIDPLRPEAKAAVEACKSSGVQVAM
                     VTGDHPATAFAISRELGLADSVDQVVTGMELKDAEAKGPEAVDALTRKGRVYARVEPQ
                     QKVLITNSLVRNGKLVAVTGDGANDAPALHAAHVGVAMGKGGTDVAKESADLIITDDN
                     FATIVSGIEEGRIVYNNLRKVIFFSISTGVAEIFLFILALIFNMPLPLLAVQLLWLNL
                     VTNGIQDVALAFEPAEGGEMNRPPRPANEAIFNRLMVEKVALVAVVVGGLTYAIFHWQ
                     MSMGRSIEEARNIAMIMMVLFENAVVFASRSELKSAFSSSPLRNPLLVFGTIAAILIQ
                     VAAMYMPGLSDVLQIHPVSLYQWVELVGVALVAFAAIELHKLIRRRFPVIG"
     gene            6061..6930
                     /locus_tag="PLV49_00360"
     CDS             6061..6930
                     /locus_tag="PLV49_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG97322.1"
                     /translation="MTIGLHIWPDNSRVVGLITKIIIILLVCLSFVSLSPCEALPEDV
                     TIMGSSTVMPLAEAAAEVFNLEQNDYLVSVTAGGTGAGILGIVERKYDIAMASRAITE
                     EEKQMFGDSFQEFKIGMDGISIAVSDEIYQEGVRDLSRDQVMRIYSGEITNWNEVRGP
                     DKDIYVISREYGSGTRDDFNEAVIGDIGTESPGVDTVAYSGAEVKTAISGSDRAIGYL
                     GFNYLGGGVQGIAFNGIVPSYENIKLDFYELKRNLYFYTFGDPTPGAKAFIDFILGPE
                     GQKIAAEEGFIAN"
     gene            6998..8041
                     /locus_tag="PLV49_00365"
     CDS             6998..8041
                     /locus_tag="PLV49_00365"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOG97323.1"
                     /translation="MHLLSGELPLCIPEPKTIWWDEGLWLIDQTQLPLKLVPLKIESI
                     RQLVAAIKSLSVRGAPALGAAGAYSIALAAERSHATNAFDMIAELEMAADMIRNSRPT
                     AINLSWGVDRALQAARGGESTEEIREAALRSAEGIAEEDIRVNRAIGKNGAKLLEDGD
                     TILTHCNAGRLACVGWGTALGVVRSAVEAGKKIEVYACETRPLHQGSRLTAWELAEDK
                     IPVTLICESMSGSLMRRGKIDKVIVGADRITRDVVFNKIGTYNHAVLAKHHKIPFYVA
                     APLSTFDRIYGEEDITIEERDPEEICTLAGVRLAPLGINVYNPAFDATPLELVSALIT
                     EKGVFRPPLMPPV"
     gene            8085..8840
                     /locus_tag="PLV49_00370"
     CDS             8085..8840
                     /locus_tag="PLV49_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOG97324.1"
                     /translation="MDAEIAVIGGVGFTLEGIADEVDTAYGPVPAVRSGIKSQRLAFI
                     PRHGTGHLPPHKVDYRAIISAAKKIGAQWVISVNSVGAMASQPVGSFFFPYDFVEFTK
                     CRSNTFYEARAVHIDLSEAYCPYLRGHLLAAAKSLDLETSEGVYVCAEGPHLETPAQI
                     RMMRQFGDVVGMTGYPEVALAREAQLCYASICIITNPAAGMGESESLSASDIPEQVKK
                     CHHDVREIIYQAAQKLDGKRSCHCRESLSEAAL"
     gene            complement(8863..>9065)
                     /locus_tag="PLV49_00375"
     CDS             complement(8863..>9065)
                     /locus_tag="PLV49_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97325.1"
                     /translation="SFEGSAKGGEGDLSYEWSSSIDGKLSTSRSFKQNPIELEKGGHI
                     IILKAMDESGKSGQGSVQVEVM"
     assembly_gap    9066..9165
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9166..9413)
                     /locus_tag="PLV49_00380"
     CDS             complement(<9166..9413)
                     /locus_tag="PLV49_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97326.1"
                     /translation="MKLVIILAICLALFSAGCMEKEDSGGAKDSAAGASEPVGHADTD
                     RIKVTITSPDAGQIIKGDEDISFEGSAKGGEGDLSYEW"
     gene            complement(9614..10246)
                     /locus_tag="PLV49_00385"
     CDS             complement(9614..10246)
                     /locus_tag="PLV49_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HOG97327.1"
                     /translation="MARRSQRKADSRKAKQWYKVVSPEMFGRVPFGETIANDPERIVG
                     RIIETTLGDLTNNFSKQNTKLKFRVDRVAGDSAYTKFLGHEMTTDYVRSLVKRRTSRI
                     DSIVDVTTTDGYTVRIKPSCFTVKRARANQVKCIRDLSKSVVMERSKNLDLNQLIQDV
                     VGGKISLDIYKEAKMIYPLRRVEVRKTEIRTEPALSMPEPAAPTAPPASS"
     gene            complement(10404..10679)
                     /locus_tag="PLV49_00390"
     CDS             complement(10404..10679)
                     /locus_tag="PLV49_00390"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOG97328.1"
                     /translation="MNASLNVEFTLSLESNPTTGYVWEATFDRAFLELKKKEFKESDG
                     ESVGSGGTETLTFVPIKAGRTEITMVHKRQWESIPLERRSFPIEITE"
     gene            complement(10728..11642)
                     /locus_tag="PLV49_00395"
     CDS             complement(10728..11642)
                     /locus_tag="PLV49_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOG97329.1"
                     /translation="MMTILLKDLGMKSKNGEKTKGMGWRPELPDLRDYTFQTKEVADI
                     LGPLKLSKVAVSTLPTSVDLKKWFPPIEDQGDLGSCTANAGVGVFEYFERRAFGEHID
                     ASRLFLYKVTRNLMQESGDTGAYIRTTMGAMALFGVPPERYWPYDISKFEEEPSAFLF
                     GFADNYKALKYVNLDPPGTTPIKLLSNIKTNIAAGIPSIFGFTVYDSISQAGSTGKIP
                     YPCSNESVQGGHAIIAVGYDDKMKIKNSNCGAETTGALMIRNSWGELWGDEGYGWLPY
                     DYVLTGLADDWWTLIKGSWIETGQFEYA"
     gene            11951..>12749
                     /locus_tag="PLV49_00400"
     CDS             11951..>12749
                     /locus_tag="PLV49_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97330.1"
                     /translation="MQKDTIIEVWETGTAAARAFEIRIAVDGEVVMNRRLNPVEAMEA
                     REISSQYASFFSNGKKGKNSYLHLLGESIFRLFFQPGWQELGPKILSGGRLIVVSSIP
                     QILLLPWELLRLPGFEDMADGSSPDFAVLRVPMEGGRPSGSCLPAPFLSQPSELRRGP
                     LRMLFFSTNLQHFEEEELSLLKAVQGLDIHLEMGETASFEELKSSIESFKPHLVYLSG
                     EAKLSGEVSGGEARLAFMDPAGKLDLRSAEEIAPVLKSGGVECIIIGG"
     assembly_gap    12750..12849
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12933..>14273
                     /locus_tag="PLV49_00405"
     CDS             12933..>14273
                     /locus_tag="PLV49_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97331.1"
                     /translation="MTEGHARGFLDRRKDLLRLYPDLRQGAVNALIITGELGRGKSAL
                     ANFLARMLAASGYFVLPLGGTAHNPMSSARLIEAAGLQLAAAGEYQGLEGTKRLLDSS
                     LPVNERLAVLMELLRSNRILIFWDDLDLEEKTGRISDPHLGELFCQMTKGLHSSRAII
                     SCPKVPANASILPRLARNWKLDRLSRAAFLRFMLQDRIIADGYRKGEIAFDALSDLYS
                     LYERDPSKLEQIRSALARGLPNDGDPLARLAECLSPESLLALQCIAVYDVAMSPAGFA
                     AAAGTDLESIAESLPLWEELSLCYKTGNLWAVHSSARPALREGLSPEEIREAHKRAGR
                     FLEEMAESGRSPELFLFRLDGLLEARGHYLEAGDLAEARRVTARISSYLDRRGYYTEL
                     IRLNREILELEPDKDSMNWIAQAYAHQMDLENAAQWYKKALDMAPDAFSYQGLGM"
BASE COUNT         3164 a         3865 c         3806 g         3138 t
ORIGIN      
        1 aggtgccggg ggcgggattc gagcccgcga cttcgagatg actcaggcgc gttcttcgca
       61 attaccctat gagtctcgcg ccctaaccag ctaggccacc ccggcacggc atagtccctc
      121 gggtgaatag ctaattaagg tttttgctta atccccttca agggaacggg catcctttaa
      181 aagctcaaag tgcaaaaggg atgagtatgc cctctgatat ctcagcctcg gtgagcgaga
      241 ccctatccgg aaagacgata gtggtgggag tgacgggaag catcgctgct gtccgggtga
      301 tagacctcat tcgcgacctc atccgcagcg gggcagaggt tcattgtacc atgagccatg
      361 ccgccggaca gatactgcat ccctatgccc tggagtatgc ctcaaaccat ccggtgataa
      421 ccgagatcac cggccgggtg gagcatgtcc agttctgcgg cgtgggcgga aaggcagacc
      481 tcttcctggt cgcgccggcc accgccaaca ccattggcaa gatggccaat ggcatcgatg
      541 atactccggt gaccaccttt gccaccacgg ctctcggaag tggcaagccc gtggcagtcg
      601 tccctgccat gcatgaggcc atgtaccgcc atcctgcagt catacggaat ctgcaaatcc
      661 tgagggaaat gggcgtggcc ctcatcgatc cgcgcatcga ggagggcaag gccaaattcg
      721 ccgataacca gcgggtggtg caggaggtag aaagactgct gggcccggcc gaccttaaag
      781 ggaagaggat cctgatcacc agcggctcga atgccgaatc catcgacccc atcagaattc
      841 tcaccaaccg ggcctcggga aagaccggtg tcgccctcgc cctggaggca cgaaggcggg
      901 gggcggatgt aaccctggtc caccgcttca tccaggacct gccagtccac caggtctatg
      961 cccagagcgc cagcgatatg ctggatgcgg tcatggcaga gctcaagaag ggctgcgatg
     1021 ccctgatcag cgccgcggca gtggcggact acaccctcga cccgagcgag gagaagatca
     1081 agtccggcct ggaattgaat ctgcggctca agcccaccag gaagataatc aaggcggtgc
     1141 gagaagccta ccctgatctg aagatcgtgg ggttcaaggc cgagaccgat accggcgacg
     1201 aggagctcct ggccagagct cgtgcatccc tggagggcgc ggggctggac ctggtagtgg
     1261 ccaatgacgt ctccagaggt ggcatgggca cagatgataa ccgggtgctg attttgagcc
     1321 ggtccggcag ccatagcgag gtcgagggaa aaaagagcct gattgcgaga aggatcatcg
     1381 actctctggc ggagattcta tgaatccccc caattcgaat catttgacaa ccagagcctg
     1441 ggtcccatcc cacatcaccg gctttttcgc ggccaggcgt gaggaagacc ctctgcagtc
     1501 cggctctatc ggctgcggcc tgtgtctttc ccccggcgcc acgaccatca tagaatcctc
     1561 tcccgatatc gaggattcaa agatcattct caatggcaga gtgacagatg ctcccgtctc
     1621 caggttcgtc atcgagcggc tggccaggag cccagtccgg gtgaaaaccg acatgaatat
     1681 gccctttggc tccggttttg gagcaagcgg ggcgggggcc ttgggttgcg cttatgccct
     1741 aaaccatcac ttcgacctgg gactcaccgc agatcagacg gcatctgtag cccatgcggc
     1801 agaggttgtc aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgggactc
     1921 accgcagatc agacggcatc tgtagcccat gcggcagagg ttgtcaaccg cactggcctt
     1981 ggggacgtca tcgcccagaa caccggcggc ctggtgattc gtctggagcc cggggcgcct
     2041 ggcatcggca gaatcgatcg aatacccgtt gcaccacaac cggttcactg cgtggttcgg
     2101 ggcccgatct ccacccggga ggtgctctcc gaccaaggca ccatgtcatc cataaatgag
     2161 gctggccggg ctgccctgaa ggatctattg aaaaagccca cattggagga gttcatgcgg
     2221 ctctcccgcc gtttcaccgt ccagacgggt cttgccagca gctgggcgct ggatatgata
     2281 gaggcggtgg aggcggaggg gggaagggcc agcatgatca tgctaggaga cgcggtctat
     2341 gcctttggcg gaattgaggc cctccagggc tcaggccagg tctatactac attcatctct
     2401 cagagaggtg ctaatcttga ctgaaattcc caaatcccat cccagatatg cttccctggt
     2461 ggcgcgggag cgcgtggcag aaggagtgaa aaaaggatat accagcaccc aggggctgat
     2521 tgcccagggc agaggagagt gcttcgacta tctcctgggc gagaggacga cggcttccgc
     2581 tgcagcagcg atccgggcgg gagcggcatt gctcctctta gccgagaggc cggcgatctc
     2641 ggtcaacggc aatgtggccg ccctcgtttc agaggagatg gtggcgctgg ctagagcact
     2701 caatatcccc ctggaggtga acctatttca ccgcagcgag gatagggtga agaagatcgc
     2761 cgatctgcta agggagaagg gggcagaaga tgttctgggc gagaggccgg acacttcagt
     2821 accaggcctt gatcatgccc gggccctggc cacccggggc ggaatctacg atgccgatgt
     2881 ggtgctcatt cccctggagg acggcgaccg ttgcgaggcc ctgctggcca tgggaaaaag
     2941 agtcattgcc atcgacctca accccctctc ccgcacagca aaaaaggcca ctgtaagcat
     3001 tgtggacaac atactccggg cggttcccca gctgacggaa gaggtcaggc agctctccaa
     3061 taagcctcgc ccggatctgg agaagatatt aaatgaatac gacaaccagg agactctggc
     3121 cgggtcggtg caggagatcc gggatcatct ggacgagcag ttcaggaagg ggagcgatta
     3181 acctatcact ggaaacctcc tgcgtatgag cttatgcagc tcaatggcgg caaaggccac
     3241 cagggccact cccaccagct ccacccactg atacagagag acgggatgaa tctgcagcac
     3301 atcggataga ccgggcatgt acatggcagc cacctggatc agaatggcgg ctatggtgcc
     3361 gaacaccagc agaggatttc taagtggact ggagctgaaa gcgctcttga gctctgatcg
     3421 gctggcgaag accacagcat tctcaaagag gaccatcatg atcatagcga tgtttctcgc
     3481 ctcctcaatg ctccggccca tggacatctg ccagtggaat atggcatagg tcaggccgcc
     3541 tacaaccact gccaccagcg caaccttctc caccatcaaa cggttgaaaa tcgcctcgtt
     3601 cgccggtctc ggcggccggt tcatctcccc gccctcggcg ggctcgaagg ccagggctac
     3661 atcttgaatc ccgttggtga ccaggttcag ccagaggagc tggacggcaa gcagaggaag
     3721 gggcatattg aagatcaggg ctaagatgaa taggaatatc tccgccactc cggtggagat
     3781 ggaaaagaag atcacctttc gcaggttatt gtagacgatc ctcccctcct ctatccccga
     3841 gactatggtg gcaaagttgt catcggtgat gatgaggtcg gcgctctcct tggccacatc
     3901 ggtcccgccc ttgcccatgg ccacgcccac atgagcggca tgcagcgcgg gtgcatcgtt
     3961 ggctccgtca ccggtaacag ccaccagctt tccgtttctc accagagagt tggtgatgag
     4021 caccttctgc tggggctcaa cccgggcgta aacccggccc ttcctggtaa gagcatcgac
     4081 tgcttccggg cctttggcct cagcatcctt cagctccatt cccgtgacca cctggtcgac
     4141 gctatccgcc agccctagct ccctggaaat ggcaaatgcg gttgccggat gatcaccggt
     4201 gaccattgcc acctggacgc ccgagctctt gcatgcctcc actgccgctt tggcctccgg
     4261 ccggagggga tcgatcatcc ccaccagtcc caaaaatgtg agcccttcca gccgatcctc
     4321 ggcgaattcc tggttctctg ggaacctgcc agaggccagg gccaggagtc tgaaacctgc
     4381 agtggccaga gcctcggcca ctttatctgc accctgtgga tccagaggaa tctcgccatc
     4441 gggagtggcc attctgctgc tcatcttcag gatcttctct ggcgagcctt tggcaaatgc
     4501 aatcctccca tccggcccct gatgaagggt ggctgagaac tggcgcttgg attcaaaggg
     4561 tatctgagcg atcctgggat aattgctctg ctcctcctgt tgagtgactc cagtctcctg
     4621 agccatcatg agtagggcca cgtccactgc atccccgtgg tagtcccagc tgccgtctct
     4681 gaagccggaa aaggcctcat ttgccagaac cgaggctctg gccagggcct tcagggcaga
     4741 tgtctggccg gctgactggc tcccgaccgg gccttctgct gccatcttcc ctgctgcttc
     4801 ctctgccact tcccgagggc ttttcgccag atcgatcaca ttccagctat ccctgccggg
     4861 aatgacagct ctggtaacag taagctggtt gacggtaagg gttcccgtct tgtccgaggc
     4921 gatgaagttg catgatccca gggcctccac cgctaccagc cggcggacga tgacatttct
     4981 ctttgccatg cgagacatgc ctatggacag agccacagtc atggccactg gcagaccctc
     5041 gggcactgcc gccaccgcca aagccaccga ctgcaggaag atatcataaa ttggctctcc
     5101 ccggtagatc tcaaccgcaa agatcaccgc cacagccacg gccacaccca gagctatctt
     5161 ggagttgaac ttctccatgc gcaggatcag aggaggcttg gtctcgggct tgccagccag
     5221 agacgctgcc agacctccta ctgcggtatt cgaggcggtg gcgaccacga cacctctacc
     5281 cctccctgat actacaatgg tgccggcaaa ggccatgttc tccctatcgc ccagtggggt
     5341 tctccgatcg agaatcagat cggggtcttt ggcatctggt atcgactctc cggtgagcag
     5401 agactcgtcc accaccagat cctggacctc tatcagtctg atatcggctg gaacctttcc
     5461 tcctgactcc aggaggacta tatcgccggg aacaagctcc tcggagtcta tctcgaactc
     5521 ctcgccatcc cggatcaccc gggtcttggt tcggaccata ttcttcaatg cttcagcgct
     5581 cttctcggct gagtactcct ggatggtgcc aatgattgag ttgagcagca gcacaatcat
     5641 gataaatatg ctctcctgcc agtctccaag caacagagag atagcggctg ccaggaggag
     5701 aatgtagatc aagggactgt aaaattggcg gagaaaatag accgcaatgc tcgtcggctt
     5761 gggtttggga aggctgttcg gcccatagat cctcaacctt tctgcaacct cgtcctttcc
     5821 cagtccgcct tcggggtttg ccttcaatgc ctctattgca tcgtatgcag gaattgaatg
     5881 ggcttctatt atcgtatcca actcaatgcc tccaccaaaa gctcctgtct ggccaggagt
     5941 tccatgggct caaattcatg aaatttttgt tatatgatct cgttgattac agtttcggta
     6001 tataagcaag atacagtcgc cttacttaat tacatgatgt gggagcgatc ggattgagct
     6061 atgacgatag gcttacatat ctggccggac aattctcgag tggtggggtt aattacaaaa
     6121 ataattataa tcttgctggt ttgtctctca tttgtatccc tctcgccctg tgaggcacta
     6181 ccggaagatg tcacaatcat gggatcctca acagtgatgc cgctggccga ggctgctgca
     6241 gaggtcttca atctcgagca gaatgattat ttggtcagtg tcacggcagg tggaaccggc
     6301 gcgggaattt taggcattgt ggagcgcaag tacgacatag ccatggcttc gcgggctata
     6361 actgaggaag aaaaacagat gttcggggac agcttccagg agttcaagat cggcatggac
     6421 ggcatcagca ttgccgtaag cgatgagata taccaggaag gagtacggga cctgagcagg
     6481 gatcaggtta tgaggatcta ttccggtgag atcaccaact ggaatgaggt gagaggaccg
     6541 gataaggata tctatgttat ttccagagaa tacggctcgg gcacaagaga cgacttcaat
     6601 gaagcggtga taggggacat agggacagaa agccctggag tcgataccgt ggcctacagc
     6661 ggagcagagg tcaagacggc aatttccgga agcgataggg ccataggcta cctgggattc
     6721 aattatttgg gaggaggcgt ccaggggata gccttcaacg gcatcgtgcc cagctatgag
     6781 aacataaagc tggattttta tgagctcaaa aggaacctct acttctatac attcggcgac
     6841 cctactcctg gagctaaagc attcatcgat ttcatcctgg ggccagaggg acagaagatt
     6901 gcggcagaag agggtttcat agcgaactga tacttttaag gagagaaaag cactctaatc
     6961 ttgcacagga gcaagcaaaa aataataata ccagtatgtg catctactat ctggtgaact
     7021 tcctttgtgc atacccgagc ccaagaccat ctggtgggat gaaggcctct ggcttatcga
     7081 tcagacccag cttcctctaa agcttgttcc cctgaagatc gagagcataa gacagctggt
     7141 agccgctata aagtctcttt ccgttcgcgg cgcccctgct cttggagccg ccggtgctta
     7201 cagcatcgct ctggctgcag aaagatccca tgccaccaac gctttcgata tgatcgccga
     7261 gctggagatg gccgccgata tgatccggaa ctcacggccc acggcgataa acctctcctg
     7321 gggtgtggac agagccctgc aggcggccag agggggggag agcactgaag agattcgcga
     7381 ggcagcgcta cgatcggcag aggggatcgc agaagaggac atccgggtca atcgggccat
     7441 aggaaagaac ggagcaaagc tcttggagga cggggacacc attctcaccc actgcaatgc
     7501 cggaaggctg gcctgcgtgg gctggggaac tgctctggga gttgtgcgct ccgcggtcga
     7561 agccgggaaa aaaatagagg tctacgcctg cgagacccga cctctgcacc agggctcccg
     7621 cctgaccgcc tgggaattgg cagaggataa aatcccggta accctgatct gtgagagcat
     7681 gtccggcagc ctgatgcgca gagggaagat cgacaaggta attgtgggag cagaccgcat
     7741 aaccagggat gtggtcttca ataagatcgg gacctacaat catgccgtcc tggccaaaca
     7801 ccacaagatt cccttttatg tggccgcacc cctctccacc tttgaccgga tttatggcga
     7861 ggaggacatc acaatagagg aacgtgatcc tgaggagatc tgcaccctgg ccggagtgcg
     7921 cctggccccc ctgggaataa acgtctacaa tccggcattc gatgccactc ctctggagct
     7981 tgtctctgca ctgataaccg agaagggcgt cttccggccc ccactcatgc ccccagtata
     8041 gattctacag aaaggtagtt taagtttgaa gagcagctca actcatggat gcagaaatcg
     8101 ccgtcatcgg gggagtggga ttcaccctgg aggggatagc agatgaggtg gacacagctt
     8161 acggtcccgt ccctgccgtt cgcagcggga taaagagcca gaggctcgcc ttcatccccc
     8221 gccatggcac agggcacctg ccgcctcata aggtagacta ccgggccatc atctctgccg
     8281 ccaagaagat tggcgctcaa tgggtcatat cggtcaatag cgtgggagca atggcatctc
     8341 agcccgtggg aagcttcttt ttcccttatg attttgtcga gttcaccaaa tgccggtcca
     8401 acacattcta tgaggcccga gcggtgcata tagacctgag cgaagcctac tgcccctatc
     8461 tccgaggcca tcttcttgca gctgcgaagt cgcttgatct ggagacctcg gaaggagtct
     8521 acgtctgcgc tgaaggcccc catcttgaga cgcctgccca gatcagaatg atgcgccagt
     8581 tcggggatgt agtgggtatg accggctatc cagaggtagc tctggccaga gaggctcagc
     8641 tgtgctatgc ttctatctgc attatcacca atcccgccgc cggaatgggg gaatctgaga
     8701 gcctttccgc cagcgatatc cctgagcagg tgaaaaagtg ccatcatgat gtccgggaga
     8761 taatctatca ggcggcccaa aagctggatg gaaaaagatc ctgccattgc agggaatccc
     8821 tatccgaggc cgcgctctag aaaagcccct ggaactgtag ccctacatca cctcgacctg
     8881 gacgctgccc tgtccggatt tgccgctctc atccatggcc ttgagaatga tgatatgtcc
     8941 acccttttcc agctctattg gattctgctt gaaggaccgg ctggtggata gctttccatc
     9001 aatgctcgag ctccattcgt agctcagatc tccttcgcca ccttttgcag agccttcaaa
     9061 gcttannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctcca ttcgtagctc
     9181 agatctcctt cgccaccttt tgcagagcct tcaaagctta tatcctcatc acccttgatg
     9241 atctgcccgg catcaggtga agtaatggtc acttttatcc tatcagtgtc agcatgtcca
     9301 acaggctctg aggcccctgc agcgctgtct tttgcgccgc cactgtcttc cttctccatg
     9361 caccctgccg agaaaagggc aaggcatatt gccaatatga tgaccagctt catgaaacga
     9421 atgatccctt agtagaatat atcttcttct atagaagatg tctttatcag aataaaagca
     9481 aagctgacca cacttatggc cgagaaattc tcaagctagc gcccttcccg caatgtacaa
     9541 gaggggcgcc aagctgatgc agattcgatc cgattgaatc atagatccaa gatggatctg
     9601 gccacaataa gccttaagat gatgccggcg gagcagttgg tgccgctggc tcaggcatag
     9661 acaatgcggg ctcagttctg atctcagtct ttcgtacctc tacccttctc aaggggtaga
     9721 tcatcttagc ttccttataa atgtccagag agatctttcc acccaccaca tcctggatca
     9781 gctgatttaa gtccaggttc ttcgacctct ccatgacgac gctctttgag agatctctga
     9841 tgcacttgac ctggtttgcc cgtgcccttt taactgtaaa gcagctgggc ttgatcctga
     9901 cggtgtagcc gtcggtagtg gttacatcta cgatggaatc aatgcgagag gttcttctct
     9961 tgaccagaga gcgcacatag tctgtggtca tctcatggcc caggaacttc gtatatgccg
    10021 agtcaccggc tacacggtcc actctgaact ttagcttggt attctgcttg gagaagttgt
    10081 tggtgaggtc tcctaaggtg gtctcgatga tccttcccac aatcctctcg ggatcgttgg
    10141 cgatcgtctc tccaaaggga acccttccga acatctccgg acttacaacc ttataccact
    10201 gtttggcctt tctgctatca gcctttctct gcgatcttct agccaataaa catcctccga
    10261 gataatatta atccgaactc cgttcaagct cactgtattt aaaccgatcg cccaaaacag
    10321 gcatgatcct tacgaaggca gtcttcgtag cggaaccttt ccatttcgag atcgcctttg
    10381 tgatgcgatt ccgctgctgt ctttcactct gtgatctcga ttgggaatga tcttctttcc
    10441 agagggatgc tctcccactg cctcttgtga accatggtta tctctgtcct tcctgcctta
    10501 atcgggacaa aggttaatgt ctctgttccg ccggatccaa ccgattctcc atcggattct
    10561 ttgaattcct ttttcttcag ctcaagaaag gctctgtcga atgttgcctc ccatacataa
    10621 ccggtagtgg gattcgattc gagcgataag gtgaactcca cgttcaagct cgcgttcatt
    10681 ggatcaccaa tgataatagg cgctaaaaag ggaaatatct ttctatatta tgcatattca
    10741 aactgcccgg tctcaatcca tgatcccttt atgagagtcc accaatcatc agccagtccg
    10801 gtcagaacat aatcatatgg caaccatcca tatccctcgt cgccccatag ctctccccag
    10861 gagtttctta tcatgagagc accggtggtc tcggcaccgc aattgctgtt cttgatcttc
    10921 atcttatcgt catagcccac tgcaattata gcatggccgc cttgcacgct ttcattgctg
    10981 cagggatagg ggatcttgcc ggtgcttccc gcctggctta tcgaatcgta aacagtgaat
    11041 ccgaatattg agggaattcc cgctgctata ttcgtcttga tgttagacag aagcttgatg
    11101 ggggtggttc cgggcggatc gaggtttaca tacttgagag ctttgtaatt gtccgcgaaa
    11161 ccaaagagga atgctgaggg ctcctcttcg aactttgata tatcgtatgg ccagtatctc
    11221 tccggtggga cgccaaatag tgccatcgca cccatggtcg tccggatgta agcaccagta
    11281 tctccgctct cctgcatcaa atttctcgtc accttataga gaaaaagcct cgaggcatcg
    11341 atatgctctc cgaatgccct tcgctcgaaa tactcaaaga ctcctacgcc agcgtttgca
    11401 gtgcatgagc ccaggtcgcc ttgatcctct atcggtggga accatttttt gagatcaacc
    11461 gatgtgggaa gggtggagac cgccactttg gaaagcttaa gagggccgag gatatctgct
    11521 acctcctttg tctggaaggt atagtctctt aagtccggaa gctctggtcg ccagcccatt
    11581 ccttttgtct tctcgccgtt ctttgatttc attcccagat ctttcaagag tatcgtcatc
    11641 atctcaacca ccgcataatg attaaagttc tccgatccta tcgcgccgtc tcatagtcga
    11701 ttcgatctga atatctgctt attttatcca atccctgagc ctttatggta atcctaatac
    11761 tattaaaata tttctaagca tatgcacgtc taaaataaca tgagctacga tcaattgcag
    11821 atcgcccatg tttttcgata aaccaattgg agaaggaatc gttacaggat aactcctgat
    11881 gctgatttat attatgcagc caactgcagg ctgagaaatt atatatcgca tccagccata
    11941 tcatccatga atgcaaaagg atactatcat tgaggtttgg gagacgggca ctgctgccgc
    12001 cagggccttt gaaataagaa tagcagtgga tggggaggtg gtcatgaaca gacggctcaa
    12061 cccggttgag gctatggagg ccagagagat atcctctcag tatgcgtctt tcttttctaa
    12121 cggcaaaaaa gggaagaata gctaccttca tctcctgggc gagagcatct tccgcctatt
    12181 ttttcagccg ggctggcagg agctggggcc gaagatccta tctgggggac ggctgattgt
    12241 ggtatcgagc ataccccaga tcctgctgct cccctgggag cttctccgcc ttccgggttt
    12301 tgaggatatg gccgatggct catccccgga ttttgccgtc ctgcgcgtgc caatggaagg
    12361 tggccggccc tccggctcat gccttcctgc accatttctc tctcagccct ctgaactgag
    12421 aagaggcccc ttgagaatgc tctttttctc caccaatctg cagcattttg aggaggagga
    12481 gctgtccctt ctcaaggcgg tgcagggatt agatatccac ctggagatgg gcgagaccgc
    12541 ctccttcgag gagctgaaaa gcagcattga gtcttttaag cctcatctgg tatacctttc
    12601 cggtgaggca aaactgtccg gcgaagtctc cggcggagag gcaaggctag ccttcatgga
    12661 tcctgcgggc aagcttgacc tgagatctgc agaggagata gctcccgttc tgaagagtgg
    12721 cggggttgag tgcataatta ttggaggggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnng agagccaaca ggtctcttcg atcccaataa gaagagagag gagataaaaa
    12901 caagatacca agagcttcct ccaatgcctg ggatgacgga aggacatgcc aggggttttc
    12961 ttgacagaag aaaagatttg cttcgactct atcccgattt gagacagggt gcggtcaacg
    13021 ctctgatcat cactggtgag cttggccgag ggaagagcgc tcttgccaat ttcctggcca
    13081 gaatgctggc cgcatcaggc tactttgtgc tgcctctggg gggcactgcc cacaacccca
    13141 tgagctctgc ccgactgata gaagcagcag gcctgcagct ggcagcggca ggagagtatc
    13201 aaggactgga ggggacaaag aggctcttag attcatcgct cccggttaat gagaggctgg
    13261 ctgtcttgat ggagcttctc cggtccaacc gcatactcat attctgggat gacctcgatc
    13321 ttgaggagaa gacgggcagg atctcggatc ctcatcttgg cgagcttttc tgtcagatga
    13381 caaaaggcct ccattccagc cgggcgatca tctcctgtcc gaaggttcct gcgaatgcct
    13441 ccattctgcc ccgtctggcc agaaactgga agctggatcg gctctcccgg gcagctttcc
    13501 tgaggttcat gctccaggat agaattatag ctgatggcta ccgcaaaggc gagatcgcat
    13561 tcgatgccct gtcggacctc tattccctct acgagagaga tccgtctaaa ttagagcaga
    13621 tccgcagcgc tctggccaga ggtctgccaa acgatggcga tcctctggcc aggctcgccg
    13681 agtgcctcag ccccgagtct ctcctggccc tccaatgcat agcggtctac gatgtagcaa
    13741 tgagcccggc aggctttgcc gccgcagccg ggaccgatct ggagagcata gccgagagct
    13801 tgcccctctg ggaggagctg tcgctctgct ataagaccgg caatctgtgg gcggttcatt
    13861 cctccgctcg ccctgccctt cgagaggggt tgagcccgga ggagataaga gaggctcata
    13921 agagggcagg ccgatttctg gaggagatgg cggaatcggg ccgctccccg gagcttttct
    13981 tgttccgtct ggacggcctc ttagaggcgc gggggcacta tctggaggcc ggcgacctgg
    14041 ctgaggcgag gagggtgacc gccaggatca gcagctacct ggatcggcgg ggatactaca
    14101 ccgagctgat caggctgaac agagagattc tggagctgga gccggacaag gattcaatga
    14161 attggatcgc ccaggcctat gctcatcaga tggatcttga gaatgctgcc cagtggtaca
    14221 aaaaggccct ggatatggct ccggatgcct tcagctatca agggctgggc atg
//
LOCUS       DAOSGP010000006        13640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_3738_length_13640_cov_17.328230, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000006 DAOSGP010000000
VERSION     DAOSGP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3738_length_13640_cov_17.328230"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..964)
                     /gene="dph5"
                     /locus_tag="PLV49_00410"
     CDS             complement(143..964)
                     /gene="dph5"
                     /locus_tag="PLV49_00410"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HOG97332.1"
                     /translation="MLHFVGLGLYDERDISLKGLEAVRAADCIYAEFYTSRLMGTTLE
                     KMEALYGRKIHPLSRQEVEVDPSWLEEGRNKDIVFLSGGDAMVSTTHLDLRLRAIKMG
                     IETRVIHSSSIVTAVSGLCGLQNYRFGRSTTIPFPYVSRGRRIIPQTPYQVLRENQKR
                     NLHTMLFLDIQMEPVERYMTANEGTALLMEMEANAGESGLMEAIAVGIARAGAPDASV
                     KADLLFRLQGYDLGGPLHILIIPARLHFMEAEALRILADAPADAVQCEVDVDTKR"
     gene            1051..1704
                     /gene="mcrC"
                     /locus_tag="PLV49_00415"
     CDS             1051..1704
                     /gene="mcrC"
                     /locus_tag="PLV49_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase I operon protein C"
                     /protein_id="HOG97333.1"
                     /translation="MIGRTTQVVDCRESMGLAKGGGLAQRGTLSEATKPDVIAIAMSP
                     GRRHITKPVCEMTYGLRREGIQTSVLVLEAGTGVPESFPQGSRGYGPTFGLSEKEVEQ
                     IARHKIAVFHLGNVRSHVIFKAKEILSQVDIPAVVVAQCPMDLEDFAKEGVRTRLVKP
                     SRQKTQTLGEVVDLVTGITRGATCNRVKLNQLAKVLNKHLAEISEREARELAKKQKR"
     gene            1743..2918
                     /locus_tag="PLV49_00420"
     CDS             1743..2918
                     /locus_tag="PLV49_00420"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOG97334.1"
                     /translation="MKLMVILGDGMADLPLGELQGKTPLKVAKKPNMDLLARLGRSGL
                     ALTVPDGFPPGSDVANLSVVGYDPRRYYTGRAPLEAASMGVHLGESDIAFRCNLVTIE
                     DGIMRDYSAGHISTQEASELIKALKPLMPKERLYAGVSYRHLLVLQKGAGAVCTPPHD
                     ISDQPVADYLPQGVDAEVLLEFMESAKSVLSGHPVNRKRIQENKRPANAIWLWGQGPG
                     PSMPSFLSLYGLTGAMISAVDLLRGIAVYAGLEVIDVPGATGTIDTNYSGKVKGALEA
                     LSRHDFAYLHVEAPDEASHEGDLEGKIRAIELLDQMVVGPVIEGLEKSGEDWRILLLP
                     DHATPISIKTHSRDPVPFVIIGRGIEPDGLDCFDEQSAKQGGYGRVDATALVRMLAA"
     assembly_gap    2983..3082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3088..4038
                     /locus_tag="PLV49_00425"
     CDS             3088..4038
                     /locus_tag="PLV49_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOG97335.1"
                     /translation="MDKLDRFRGCLLGGAAGDALGMPTEGYTAGEIQSIFGRIVDMMP
                     PPEGHFHAGLRAGQFTDDTEETLLLAESLIEHSGFSADGFSSKLASWGSSWCLNERLR
                     SGVGFATRSAIMSMIAGTPWQESGISIPTCGSAMRAAPIGLLYHADLNLVRSYADLQG
                     LPTHSAPASRAGAIAVAVGVALCLIDLPKEMILESAARQAGRVDRELAERLIWVQSLL
                     DLPPEEAMGLIRNSPLSKETVVSAFYCFLRFRPEEGLIAAASGGGDTDSIASIAGNLF
                     GAAYGASWIPQRWLVSLEQRERIEGIAKELFHLCESCCPQ"
     gene            4078..5103
                     /gene="argC"
                     /locus_tag="PLV49_00430"
     CDS             4078..5103
                     /gene="argC"
                     /locus_tag="PLV49_00430"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOG97336.1"
                     /translation="MIDVGIIGGSGYTGGELLRLLANHPEVDVKLVTSRKLSGKPVSL
                     VHPHLKSIYALNFEALNAKEAAERCDIIFTGVPHGTAMDWVPDLLDAGTRVIDLSSDY
                     RLPVDVFEKTYGMKHRAPRDAIFGLPELHSEVAEAKLVGNPGCYPTGAILALAPLARA
                     GLVDRVVYDSKSGISGAGIEPTETSHYPNMSQNVIPYKITTHRHVPEIKQELGLLSPG
                     IKVNFTPHVIPSVRGILTTAHVFAKEEAETTLQDREAVARIYRDFYKDAPFVRLADGA
                     PRLSAVRGSNFCDISFEPEKGSDRIVVISAIDNLVKGASGQAIQNMNLMAGLDQRTGL
                     WFPGMAP"
     gene            5135..5617
                     /locus_tag="PLV49_00435"
     CDS             5135..5617
                     /locus_tag="PLV49_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG97337.1"
                     /translation="MKVRDVMNVMPVTVQASANVSEAARLLRENKISGMPVLDGERLV
                     GIVSESDLLRLLSVEDESEGSLWLPSPFEIFEVPFRDLVKWERMRSSLKEIPEKEVAD
                     VMSRNLHEIGPDDSIEEAASIMTRHRINRLPVVEDGRLVGIVTRGDIISGLGMAHAED
                     "
     gene            5604..6767
                     /gene="argJ"
                     /locus_tag="PLV49_00440"
     CDS             5604..6767
                     /gene="argJ"
                     /locus_tag="PLV49_00440"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOG97338.1"
                     /translation="MQRIEGGICAIDGVRACGVRAGKYGLALIAASGTAAGMFTTNRV
                     KAAPLAVTAEHLAKGHLDGIIANSGCANAYTGPRGVEDARRMASLLAGFLKSDEERIA
                     VASTGVIGRYLDMDIIGRLFEEARGHLRSDGEAGLEAERAIMTTDTREKEVAVEHQGL
                     RVAGIVKGAGMIEPNMATMLCFLFTDADFSPEVLKSCLTDAVRDSFNMLTVDGDTSTN
                     DIVLLTATGRAQGRIEDFQQALRYVCVDLARQMARDGEGASKFFETLVTGARNEADAR
                     LAAKAVAKSSLVKTAVYGADPNWGRIICAVGYSGAEMDPDLVTLGLEGSGLKVDLVWK
                     GRIAEEVLDKAREIMSGEEIIINIDLGLGMASARSFGCDLTHEYVNVNANYTT"
     gene            6769..8235
                     /gene="argH"
                     /locus_tag="PLV49_00445"
     CDS             6769..8235
                     /gene="argH"
                     /locus_tag="PLV49_00445"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOG97339.1"
                     /translation="MFRDQRLGEISEDILDYLSSRKADQRIFLADLLVDRAHLVMLKE
                     RGLISEEVALKIIAALDEIEEGSALRAGEDVHEAIEAAVIVLVGPEGGRMHTGRSRND
                     EVATCIRIALRDDLLDLMAEELSFIKTLVRLAANHRESVIPGFTHTQHAQPTTLAHHL
                     LAHADAALRDLGRLKDAYRRVNKSPLGAAAFASTGFDIDRARTADLLGFDGLVENSMD
                     AVSSRDFVLEVLAALSIAMVNCSRLAEELILWSSSEFGYLELDNLYAATSSIMPQKKN
                     PDTAELARAKTGSVIGSLVAALSICKALPMSYNRDLQEVTPHLWRSLDWTQSTVRILD
                     GCLSSLRFNLERLKESSVLGFSTATELADSLVRITGMPFRTAHSIVGRIAAGKGEPTM
                     RTLDEISLEIWGSPASERGFSNDHLLRALDPVSNVALRANAGGPAPEETARMIEERMR
                     LIAEEEKNLAGMRSRVDGAIEVLRAIGRSDVLRDLKGD"
     gene            8241..9149
                     /locus_tag="PLV49_00450"
     CDS             8241..9149
                     /locus_tag="PLV49_00450"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG97340.1"
                     /translation="MSTLVTGGAGFIGSNLVEDLLRAGEDVLVLDNMHTGSAENLAGL
                     EGRLELIQKSCNDLSTLDIHPQKIYHLGIPSSSPMYKRNPYLVGEAINGFISVFELAR
                     RSGSRVVYASSSSLYNGILPPHREDMAIGVADYYTEARLAMERMAELYQRLYGIASVG
                     LRFFSVYGPREEAKKQYANMVTQFLWDMKAGKRPLVYGDGTQTRDFTYVLDVVRAMRL
                     AMDSDYHGILNVGTGEAWSFNEVIELLNRMMDLSLEAQYTENPIKNYVLQTLADTAKA
                     ERTIGFKSRFGLKEGIGELIRNFLPP"
     gene            9146..9850
                     /locus_tag="PLV49_00455"
     CDS             9146..9850
                     /locus_tag="PLV49_00455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG97341.1"
                     /translation="MNLLSLYHNVDLENRKTVLDLIDVNERAKIMDLGCSDGEFALRI
                     AQKAKTLNIFGVEFLKEAAQRARSRGIRVCQADLNEILPLAAESFDVVHANQVLEHLS
                     ETDRFIKEVHRILKPGGYAVISTPNLAGLHNILSLVFGKQPFTAHVSNEVIVGNSLDP
                     KCGMLHASRGEVHLRIFTPESLRELFRYHGFDVEQLIGVGYYPFPCPLASVLSRLDKT
                     HSVYLTMKVRKAIKMI"
     gene            9852..10952
                     /locus_tag="PLV49_00460"
     CDS             9852..10952
                     /locus_tag="PLV49_00460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG97342.1"
                     /translation="MKILQTPARIFATGGVESYVRNLSRELVGMGHDVGVICADGPGE
                     NDIDQRIHTKTLKSRGKIANTSINPYLPLALYREDFDILHTHLPTPWSADWSGLISRL
                     KKRPLVLTYHNDIVGEGWAGQIAWIYNKTALKLLMKSAGGIIVTRNRYVSPYLKDYAG
                     KVFFVPLGIDDDAFRPKEAPPKGDIFFLSVLDEFHKYKGLEVLFAALKIMKQELPDVR
                     LVVGGRGCMFDHYRRMADSLGIGDNVSFAGFIPSKKLIEYYNGCKLFALPSTDPRREG
                     FGIVPLEAMACERPVVVTEIMGMAGDINECGAGMVARCNDKEALASSMLAILKDDDLA
                     RRMGAEGRKLAIGKYSWRRAAEQIERVYRELL"
     gene            10955..11899
                     /locus_tag="PLV49_00465"
     CDS             10955..11899
                     /locus_tag="PLV49_00465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG97343.1"
                     /translation="MELISVIVLNYNGKGFLNSCLSSLASQTYSDFEVIVVDNGSRDG
                     SPEYIEENYPWVRLAKNDENLGFAGGTNVGIRAAKGKFIITLNNDSMADRRFIEELIK
                     PMADPEVGVCAAKMLFPDGRINSAGICISRSGAAWDRGMFEPDRGQYEFVEEVFGACA
                     GAALYRREMLDEIGLFDEDFFLYLEDVDLAFRARLAGWKCLYVPGARVIHHHGGTAGV
                     GSDLAVYYGNRNIVWYPIKDFPFRLLITSLPFIVARNLAVIPYYALRGQGGVILKSKL
                     DALKGVVKMMEKRKDVVRRADYSQINRFVEIWGRMKGH"
     gene            11917..12678
                     /locus_tag="PLV49_00470"
     CDS             11917..12678
                     /locus_tag="PLV49_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG97344.1"
                     /translation="MWIFEYRELIKILTISDLKVKYQSSVLGFAWSLLNPLLMMLVLY
                     LVFSNVFNANQDNFALYLLIGIVSWRFMAIGSSAAMGAIVGKPSLVTKIYIPRQVLVT
                     SVVLSNLISSILEFLVLVPLLIILGAGISPYILLFPFIHMIYFLIVYGISLILAALYV
                     YYRDLNQIWDVLIQIGFFLSPIVYPLSTVPPKYLDYYMLNPMTALIQMYRDVLLYHQL
                     PAPQDVAITIAAGLFIMVVGTLVFKKLERRFAEEI"
     gene            12681..13505
                     /locus_tag="PLV49_00475"
     CDS             12681..13505
                     /locus_tag="PLV49_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG97345.1"
                     /translation="MPLPLAEINKGLDKLNLVKGGIGRGTNGNAIVVEGISKNFRIPK
                     EKKLTVFEHLAGLVGRGTSSYDEFMALQNVSFAVKHGETFGVIGPNGCGKSTLLKVLA
                     GVLYPDSGRVEINGKIAPFLELGVGFQPELSARDNVYLYGAIMGLTHKEVDQRYDEIM
                     EFSELQRFENMKLRNFSSGMYVRLAFATAIQTNPDVMLVDEVLSVGDESFQKKCSDKI
                     DEIRKAGKTILLVSHSLATVRSLCSRCMLMNRGQVVALGETGGVLAEYERMRGAGT"
BASE COUNT         3007 a         3399 c         4120 g         3014 t
ORIGIN      
        1 gtgaccttgg gcataaatcc tctctatgga aatcgatctt gggcgcaagc tggcaaggcg
       61 ccttcttgca gcatggccca aagaatactc cgggcttatt aatagctttt catggcggca
      121 gataagatcc cgattaatgg gctcatcgct tcgtatccac atccacttcg cactggacgg
      181 cgtctgcggg tgcatccgcc agaatccgca gagcctctgc ctccatgaag tgcagtcttg
      241 cagggatgat caggatatgc aatggaccgc cgaggtcata gccctgcaga cgaaagagga
      301 ggtcagcctt cactgaggcg tcaggggcgc ctgccctggc gatgcccaca gcaatcgcct
      361 ccataagacc gctttctcct gcattggcct ccatctccat gagcagagct gtcccctcat
      421 ttgccgtcat gtacctctca accggctcca tctggatgtc gaggaatagc atggtatgga
      481 gattgcgctt ctggttctcc ctcaacacct ggtatggtgt ctgaggaatg atcctgcggc
      541 cccgggagac gtaggggaag ggaatggtgg ttgatcggcc gaaacggtag ttttgcagtc
      601 cgcacaggcc ggacacggct gtgacaatgg agctggaatg aatcacgcga gtctcaattc
      661 ccatcttgat cgctctgagc ctcaggtcta ggtgggtggt ggagaccatg gcgtcgcccc
      721 cggagagaaa gactatatcc ttatttcggc cttcctccag ccaggatggg tccacctcca
      781 cctcctgtcg cgagagggga tggatctttc gcccatagag cgcttccatc ttctccaatg
      841 tggttcccat cagccgggag gtatagaact cggcgtagat gcagtctgcg gcgcggaccg
      901 cctccaggcc tttgagagat atatctcttt catcataaag cccgagacca acaaagtgga
      961 gcatggttct tcctggggtc gccggtttaa aaaaactccc tctggaggca gttcgtcaga
     1021 tctatattcc atccctgtag agatggaggt atgattggca ggacaactca agtagtcgat
     1081 tgccgggaga gcatgggtct ggccaagggg ggcggattgg cccagagggg cacactctct
     1141 gaggcgacaa aaccggatgt catcgctata gctatgtcac cgggacggcg ccatataact
     1201 aagcccgtct gcgagatgac atatggcctg cgcagggagg ggatccagac cagcgttctg
     1261 gttttagagg ccgggaccgg ggttccggag agctttcctc aagggtcgcg aggttacggc
     1321 ccgaccttcg gcctgtccga gaaagaggtg gagcagatag cacgccataa gatcgccgtc
     1381 ttccatctgg gaaatgtgcg atcccatgtg atctttaagg cgaaggagat cctctctcag
     1441 gtggacattc ccgcggtggt ggtggcccag tgtccgatgg acctggagga cttcgccaag
     1501 gagggggtga ggaccaggct ggtcaagccc tcccgccaga agacgcagac ccttggggag
     1561 gtggtggatc ttgtcaccgg catcaccagg ggggcgacct gcaaccgggt caagctcaac
     1621 cagctggcca aagttttaaa caagcatctg gcggagataa gtgagcgcga ggctcgagaa
     1681 ctggccaaaa agcaaaagcg atgaatggat tttaatacta ttttttcata gggatggcgc
     1741 gaatgaagct gatggtgatt ttaggggacg gtatggccga tctccccctg ggggagctgc
     1801 agggaaaaac tcccctaaag gtggcaaaaa agcccaatat ggacctactg gcacggctgg
     1861 ggagaagcgg actggcattg acagtcccgg atggatttcc accgggcagc gacgtagcca
     1921 acctctcagt ggtcggttac gatcctcgca ggtattatac cggccgcgct cctctggagg
     1981 cggcctccat gggagtgcat ctaggtgaga gtgatatcgc ttttcgctgc aacctggtca
     2041 ccatagaaga tggcataatg agagattaca gcgccgggca catcagcacc caggaggcca
     2101 gtgagctgat caaggcttta aagcccctga tgccgaaaga gagactatat gccggtgtta
     2161 gctaccgaca tcttctggtc ctgcaaaagg gggcgggagc agtctgcaca ccgcctcatg
     2221 acatcagcga ccagccggtg gctgactatc tgcctcaggg ggtggatgca gaggtccttt
     2281 tggagtttat ggagtcggcg aaatccgtgc tgtccggcca tccggtgaac cggaaaagga
     2341 tccaagagaa taagaggcca gccaatgcca tctggctgtg gggccagggg ccaggaccat
     2401 cgatgccctc cttcctgagc ctatacggcc tgaccggggc catgatctct gccgtggatc
     2461 tcctccgggg catcgccgtt tatgccggat tggaggtgat agatgttccc ggagccacgg
     2521 gaacgattga caccaactac tccggcaagg tgaagggagc tcttgaggct ctctcccgtc
     2581 atgacttcgc ctatctgcat gtcgaggccc cggacgaggc cagccacgag ggcgacctgg
     2641 aggggaagat ccgggctatt gagcttctgg accagatggt ggtgggaccg gtgatcgagg
     2701 ggctggaaaa gagcggagag gactggcgca ttctcctcct gcccgatcat gctaccccca
     2761 tatccataaa gacccacagc cgggatccgg ttccttttgt catcatagga cggggcatcg
     2821 agccggacgg cttagactgc tttgatgagc agtcagctaa gcagggtggg tatggcaggg
     2881 tcgatgctac cgctctagtc cgaatgctgg ccgcataatg gctctgctgg atatggataa
     2941 gctggatcgc tttcggggct gtctgttggg gggtgcagca ggnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nntggatatg gataagctgg atcgctttcg gggctgtctg
     3121 ttggggggtg cagcaggaga cgcactgggg atgcctacag aagggtatac tgcaggcgag
     3181 atccaatcca ttttcggtcg gatagtggat atgatgcccc ctcctgaggg gcattttcat
     3241 gctggcctcc gggccggcca gttcactgat gatacagaag agactctgct cctcgccgag
     3301 tcgctaattg agcattccgg cttctcagca gacggttttt ccagcaagct cgccagttgg
     3361 ggatctagct ggtgcctgaa tgaaaggctc agaagcggag tgggatttgc cacccgttct
     3421 gcaataatga gcatgatcgc cggcacaccc tggcaggagt cgggcatcag catacccaca
     3481 tgcggatcgg ccatgcgcgc ggctcccatc ggtctgttat accatgccga cctgaacctg
     3541 gtcagaagct atgccgatct gcagggcctt cccacccatt ccgcacccgc ctcacgggca
     3601 ggtgcgatcg ctgtagcagt gggcgtcgcc ctctgtctta ttgacctccc caaggagatg
     3661 atccttgaga gtgccgcccg ccaggccggc cgggtggatc gggaactggc agagcgtctc
     3721 atttgggtcc aatcccttct cgatcttccg ccagaggagg ccatgggcct gatcagaaat
     3781 tctcctctat ctaaagagac ggtggtctct gcattttatt gctttttgcg attccggccg
     3841 gaggaggggc ttatcgctgc agccagcggc ggaggtgaca ccgattccat cgcctccatc
     3901 gctggaaacc tattcggtgc agcttatggt gccagctgga taccgcagcg ctggctcgtg
     3961 agcctcgagc agagggagag gattgaaggc atcgcaaagg agcttttcca tctttgtgaa
     4021 tcctgctgtc cccaataggc gcggcgaatt tatatcagta gctctctggg aaccttcatg
     4081 atagatgtag gaattatcgg tggatcgggc tacaccggtg gggagctctt gcgtctcctt
     4141 gcaaaccatc ccgaggtcga tgtgaagctt gtcacctccc gcaagctctc gggaaagcct
     4201 gtgagcctgg ttcatcctca tcttaagagc atttacgctt tgaatttcga ggcgctcaat
     4261 gcaaaagagg cggctgagcg ctgcgatata atcttcaccg gggttcccca tgggacggca
     4321 atggattggg tgcccgatct cctggatgcc gggacgcggg tgatcgatct atcctccgat
     4381 taccgcctgc cagtggatgt tttcgagaag acctatggca tgaaacaccg cgctccaagg
     4441 gatgctatct ttggcctgcc ggagcttcat tctgaggtgg cagaggcaaa gcttgtcggc
     4501 aaccctggct gctatcctac gggagccatc ctggccttgg cacctctcgc ccgtgccggg
     4561 ctggtggatc gggtggtcta tgactccaaa tcggggatct ctggcgcagg cattgagccc
     4621 acagagacca gccactatcc caatatgtcc cagaacgtta ttccctataa gatcactacc
     4681 cacaggcatg tcccagagat aaagcaggag cttgggcttc tctcgcctgg gatcaaggtg
     4741 aacttcaccc cccatgtcat tccctcagtc cgcggtattc tgaccactgc acacgtattt
     4801 gccaaagagg aggcggagac caccctgcag gatcgtgagg ccgtggcccg gatataccgg
     4861 gatttctata aagatgcccc cttcgtccgc ctggcagatg gtgccccgcg cctcagcgct
     4921 gttcggggat ccaacttctg cgatataagc ttcgagccgg agaagggcag cgacagaatc
     4981 gtggtcatat ccgccataga caacctggtg aagggcgcat ccggccaggc catccagaac
     5041 atgaacctca tggcgggact ggatcagagg actgggttgt ggttcccggg aatggcgcca
     5101 taaaaagagg caaacggaga agaggtggat acaaatgaag gtcagagatg tgatgaacgt
     5161 catgccggtt acggtgcagg catcggcgaa cgtgagcgag gcagccaggc tcctcaggga
     5221 gaacaagatc agcgggatgc cggttttaga tggtgagagg ctggtgggaa ttgtatcaga
     5281 gtcggacctt ctccgtctct tatcggtgga ggacgagtct gaggggagtc tgtggctccc
     5341 cagccccttc gagatattcg aggttccctt tcgggacctg gtgaaatggg agcggatgcg
     5401 gtccagtttg aaagagatcc ccgaaaagga ggttgctgat gtgatgagcc ggaacctgca
     5461 tgagatcggg ccggacgact ccatagagga ggcggcaagc attatgaccc gccaccggat
     5521 caaccgcctt ccggtggtcg aggacggtcg gttggtaggt atagtgaccc gtggcgatat
     5581 catatccggt ctggggatgg cccatgcaga ggattgaggg cgggatatgc gccattgacg
     5641 gggtgcgcgc ctgcggagtg cgggcgggca agtacggcct ggctctaatt gctgcttctg
     5701 gaacagctgc cggcatgttc accaccaacc gggtaaaggc tgctccgctg gctgtcaccg
     5761 cagagcatct ggccaaaggc catctggatg gcataatcgc taatagtggc tgcgcgaacg
     5821 cctacaccgg gccgcggggg gtagaagatg ccagaaggat ggcctctcta ttggccggat
     5881 ttctgaagag cgatgaggag cggatcgccg tggcctcgac tggggtcata ggccgttatt
     5941 tggatatgga tatcatcggc cgcctcttcg aggaggcaag agggcacttg aggtctgatg
     6001 gggaggccgg cctggaggcg gagagggcga tcatgaccac cgacaccagg gagaaggagg
     6061 tggctgtaga gcaccaggga ctccgggtgg caggaatagt caagggtgcg ggcatgatcg
     6121 agcctaatat ggctactatg ctatgctttc tctttaccga tgccgacttt tccccggaag
     6181 tgctcaagag ctgcctgact gatgccgtcc gagatagctt caatatgctc accgtggatg
     6241 gagatacaag caccaatgat atagtcctcc tcaccgcgac cggacgggcg cagggcagga
     6301 tagaggactt ccagcaggct ttgcggtacg tctgtgtgga tctcgcccgc cagatggcca
     6361 gggacggaga gggagcgagc aaattcttcg agaccctggt taccggggcc cgcaatgagg
     6421 ccgatgcccg gctggcggcc aaagcggtgg caaagagcag ccttgtcaag accgcggtct
     6481 atggggcgga tcccaactgg ggaaggatca tctgcgctgt ggggtactcc ggggcagaga
     6541 tggacccgga cctggtcacc ctggggctgg aaggctcggg gctgaaggtc gatctcgtct
     6601 ggaagggccg gattgcagag gaggtcctgg acaaggcccg agagatcatg tccggggagg
     6661 agatcataat caatatagac ttgggcctgg gaatggcaag cgcgcgcagc tttggctgcg
     6721 acttgaccca tgagtatgtg aatgtgaacg ccaactatac cacctgatat gttccgggat
     6781 cagcgtttgg gggagatcag cgaggacatt ctcgattatc tgtccagccg taaggctgat
     6841 cagaggatat tcctggccga tctgctggtg gacagggccc acctggtgat gctgaaagag
     6901 aggggcctga tatccgaaga ggtcgcattg aagatcattg cagctctgga tgagatcgaa
     6961 gagggatctg cccttagggc tggagaggac gttcatgagg ctattgaggc tgcagtcatt
     7021 gttctggtgg gtcctgaagg ggggcgaatg cataccggcc ggtctcgtaa cgatgaggtg
     7081 gcgacatgca ttcgcatcgc actgagggat gatctgctcg atctgatggc agaggagctc
     7141 tctttcatca agacgctcgt ccgcctggcg gcgaatcaca gggagtccgt catccctgga
     7201 ttcactcaca cccagcatgc tcagcccacc accctggccc atcatctact ggcccatgcc
     7261 gatgctgctc ttcgggatct gggtaggcta aaggatgcct ataggagggt caataagagc
     7321 cccctgggcg cagctgcctt cgcctccact ggatttgata tcgatcgtgc ccggactgct
     7381 gatctcctgg gatttgacgg cctggtggag aacagcatgg atgctgtctc cagccgggat
     7441 ttcgtcctgg aggtcctggc tgcactctcc attgccatgg tcaattgcag ccgcctggca
     7501 gaggagttga tcctctggtc ctcatccgag ttcggctatc tggaattgga caatctctat
     7561 gctgccacca gctccatcat gccccagaag aaaaatccgg atacggcgga gctggcccgc
     7621 gccaagaccg gctcggtgat aggatctcta gtggccgctt tatccatctg caaggctctg
     7681 ccaatgagct ataaccggga ccttcaggag gtgacccccc atctgtggcg aagcctggac
     7741 tggacgcaga gcacagttag gatcctggat ggatgcctat cctcattgcg gttcaacctt
     7801 gagcgtctta aagagagttc agtcttgggt ttttccaccg ctaccgagct ggccgactca
     7861 ctggtgagaa tcacaggaat gccatttcgc accgcacata gtatcgtggg ccggatcgcg
     7921 gccggaaagg gtgagcccac aatgaggacc ttagatgaga tcagtctgga gatctggggg
     7981 tctcctgcca gcgaacgggg cttctccaat gaccatctgc tgagggcgtt ggacccggtg
     8041 agcaatgtgg ctctgcgagc gaacgccggc ggccctgctc cggaggagac tgcaaggatg
     8101 attgaagaga ggatgagact gatcgcagag gaggagaaga atctggccgg aatgcgaagt
     8161 cgagtggacg gagcgattga ggttctgcgg gccattgggc ggtcggatgt cctaagggac
     8221 ttgaaaggag attgatgact atgagcacac tggtgacagg tggcgcgggt tttatcggct
     8281 cgaatctggt ggaggatctc ttgcgggcag gagaggatgt gcttgtactg gataatatgc
     8341 acaccggaag cgctgagaac ctggcgggtt tggagggccg cctggagctg atccagaaga
     8401 gctgcaacga tctgtccacc ctggatattc atccccagaa gatctaccat ctgggaatac
     8461 cctcatcctc gcccatgtac aagaggaatc catacctggt gggggaggcc attaacggct
     8521 ttatctcagt cttcgagctg gcccgaaggt caggatcgag ggtggtctat gcctcatcct
     8581 cctctcttta caatggtatt cttcctcccc accgcgagga tatggccatt ggggtggccg
     8641 actactacac cgaggcccgg ctggccatgg agcgcatggc agagctttat cagaggcttt
     8701 acggcatagc ctcggtgggc ttgaggttct tttccgttta cggtccccgg gaggaggcaa
     8761 agaagcagta tgcgaacatg gtcactcagt tcctctggga tatgaaagcg ggcaaaaggc
     8821 cgcttgtata cggagatggc actcagacca gggatttcac ctatgttctg gatgtagtgc
     8881 gggcaatgcg gctggccatg gattcggact accatgggat actgaatgtg ggaacgggcg
     8941 aggcctggag cttcaatgag gtgatagagc tcttgaaccg gatgatggat ctgtccctgg
     9001 aagcgcagta caccgaaaat cccataaaaa actatgtcct gcaaaccctg gctgataccg
     9061 ccaaagcgga aaggacgatt ggattcaaat cgaggttcgg cctgaaagag ggaatcggag
     9121 agctgatccg gaattttctg ccgccatgaa tctgttatct ttataccata acgtcgatct
     9181 agagaaccgc aagactgtac tggacctgat cgatgtgaac gagagggcga agataatgga
     9241 tttggggtgc tctgatggcg agtttgccct gaggattgcc cagaaggcga aaacgttgaa
     9301 catcttcgga gtcgagtttt taaaggaggc tgcgcagcgg gccaggagcc gaggaatcag
     9361 agtctgccag gcggatctga acgaaatcct gcccctggca gcagagagct ttgatgtcgt
     9421 tcatgccaat caggtgcttg agcacctgtc cgagacagat cggttcatca aagaggttca
     9481 cagaattctc aagcccggtg gttatgctgt catatctaca cccaatctcg ccggtctgca
     9541 taacatatta tccttagttt ttggcaagca gccgttcact gcccatgtga gcaatgaggt
     9601 gattgtgggc aactctctag accccaaatg cggaatgctt catgcgagca ggggtgaggt
     9661 tcatttgagg atcttcaccc cagaatctct gcgagagctg ttcaggtacc atggatttga
     9721 tgtggagcaa ctgattggag tggggtatta tccctttccc tgtccgttgg cctcagttct
     9781 ctcgaggctg gataagactc attccgtcta tctgacaatg aaagtgcgca aggcaataaa
     9841 aatgatctaa catgaaaata ctacagaccc cggcgaggat atttgccacc ggtggagtgg
     9901 agagctatgt tcgcaacctc tccagagagc tcgtcgggat ggggcatgat gtgggggtga
     9961 tatgcgcgga cgggccgggg gaaaacgata ttgatcagcg tattcacaca aaaacactga
    10021 aaagcagggg gaaaatagcc aataccagca tcaacccgta tttgccattg gccttgtata
    10081 gggaggattt cgatatcctt cacactcatc ttcccactcc ttggagcgct gactggagcg
    10141 gcctgatctc acggttaaag aaaaggcctc ttgtcctgac atatcataat gacatagtgg
    10201 gtgaaggatg ggcaggccag atcgcctgga tctacaataa aaccgctctg aagctgctga
    10261 tgaagtccgc cggcggcatc attgtgaccc gaaaccggta tgtatcgcca tatcttaagg
    10321 attatgccgg caaggtcttc ttcgtacccc tgggtataga tgatgatgcc ttcaggccca
    10381 aggaggctcc cccgaaaggc gatatatttt tcctcagcgt cctggatgag ttccataagt
    10441 acaaagggct ggaggtcctc tttgctgcct tgaagatcat gaagcaagaa ttgcccgatg
    10501 tgcgactggt ggtaggaggg aggggctgca tgttcgacca ttataggcgt atggccgact
    10561 ctctggggat cggtgataat gtgagcttcg ctggctttat cccctccaag aagctcatcg
    10621 agtactacaa tggatgcaag ctttttgccc tcccctccac cgatcccaga agggagggtt
    10681 ttgggatcgt tcccctggag gcaatggcct gtgaaaggcc ggtggtcgtc accgagatca
    10741 tgggcatggc cggagacatt aatgaatgtg gggcaggaat ggtggcaaga tgcaatgata
    10801 aagaggctct ggcatcgtcc atgctcgcca tcctgaagga tgatgacctg gcaagaagga
    10861 tgggagccga aggcaggaag ctcgcaattg gaaagtatag ctggcgaaga gcagcagagc
    10921 agattgaaag ggtataccgg gaattgctct agagatggaa ttgattagcg taatcgtgct
    10981 gaattataat ggcaaaggat tcctgaatag ctgcctctcc tccctcgcct cccagaccta
    11041 ttcggatttt gaagtgatag tggtggataa tggatcgagg gacggcagtc ctgagtatat
    11101 cgaggaaaat tatccctggg tcaggctggc caaaaacgat gagaacctgg ggtttgccgg
    11161 tggaaccaat gtcggaatca gggcagcgaa agggaaattc atcatcaccc ttaacaacga
    11221 ttccatggcg gaccgccgat tcatagagga gctgatcaag cccatggccg atcccgaggt
    11281 cggagtttgc gctgcaaaga tgctgtttcc cgatgggcgc atcaactctg ccgggatatg
    11341 catctccagg agcggagctg cctgggaccg ggggatgttc gagccggatc gagggcagta
    11401 tgaattcgtg gaagaggtat ttggagcctg tgcgggagcg gccctctaca gaagggagat
    11461 gctggatgag atcggtcttt tcgatgagga tttcttttta tacctggagg atgtggatct
    11521 ggcctttcgg gcccgtctgg ccggctggaa gtgcttatat gtgcctggtg ccagggtaat
    11581 tcaccaccat ggaggaactg ccggagtggg atcggatctg gccgtctatt atggcaacag
    11641 gaatattgta tggtatccga tcaaggattt tcctttcagg cttctgatca cctctctgcc
    11701 ttttatagtg gccagaaatt tggcagttat cccctactat gctctgcgcg gccagggtgg
    11761 ggtcatcctt aaatcgaagc tcgatgctct gaagggcgtc gtaaagatga tggaaaagcg
    11821 aaaggatgtc gtgcgccggg cagattattc ccagatcaat aggttcgttg agatctgggg
    11881 caggatgaag gggcattagg agaaagaggc attaagatgt ggatctttga atatagggag
    11941 ctgatcaaga ttctcaccat cagcgacctt aaggtaaagt accagagctc cgttttgggc
    12001 tttgcctggt cgctcttgaa tccgcttctt atgatgctgg tcctctatct ggtattcagc
    12061 aatgtgttca atgctaatca ggacaatttc gctctttacc tgctgatcgg catcgtaagc
    12121 tggcgattca tggccatagg aagctcagcc gccatggggg ctatcgtggg caaacccagc
    12181 ctggtgacca agatatatat cccccgccag gttctggtga cgagcgtagt tctgtccaac
    12241 ctgataagct caattctgga gttcctggtg cttgtgcccc tgctcattat ccttggcgcg
    12301 ggaatctcgc cttatatcct gctcttccct tttatacata tgatttattt ccttatcgta
    12361 tatggaataa gcttaatcct ggcagctctc tatgtctatt accgcgatct taaccagata
    12421 tgggatgtgc tcattcagat cggatttttc ctctcgccga tagtctatcc cctcagcact
    12481 gtgccgccga agtatctgga ctattatatg ctcaatccca tgaccgcatt gatacagatg
    12541 tatagggatg tgctgctcta ccatcagctg cctgcaccgc aggatgtggc cataacaatt
    12601 gcagctggtc tgttcatcat ggttgttggc actttagtct tcaagaagct ggagcgcagg
    12661 tttgcggagg agatctgaat atgcctctgc ctctcgccga gattaataag ggcctggata
    12721 agttgaacct ggtgaagggc ggcataggcc ggggcactaa cggcaatgca atagtggtgg
    12781 agggcatctc caagaatttc agaataccga aagagaagaa gctcaccgtc ttcgagcacc
    12841 tggccgggct ggtggggagg ggcaccagct cttatgatga gttcatggcc ctgcagaatg
    12901 tcagcttcgc tgtgaaacat ggcgagacct ttggggtgat cgggcccaac ggctgcggca
    12961 agagcactct gctcaaggtt ctggcgggag ttctctatcc ggatagcggc agggtggaga
    13021 taaacggaaa aatcgcgcct tttctggaat tgggagtcgg ctttcagccg gagctctcag
    13081 cccgggataa cgtctaccta tacggggcaa taatgggcct gacccataaa gaggtagacc
    13141 agagatacga tgagataatg gagttctctg agctgcaacg cttcgagaac atgaagctgc
    13201 gcaacttctc ctcggggatg tatgtgcggc tggcctttgc cactgccatc cagaccaatc
    13261 cggatgtcat gctggtggat gaggtcctat cggtggggga tgagtccttc cagaagaaat
    13321 gcagcgataa gatagatgag attcgcaaag cgggcaagac catcctcctg gtctctcact
    13381 ctttggccac ggtgcgttcg ctatgcagcc gctgcatgct catgaaccgc ggccaggtag
    13441 tggccttggg ggagactggg ggggtcttgg cggagtatga aaggatgagg ggggctggca
    13501 cataaggcat atgaatgagt cccctggaag gtgaagggat gcagctatgg ccagacagcc
    13561 ggtgattctg tcacaaaaat cctcggcctg gggagatatc cacttttcgt ctccggtctc
    13621 cctctcgtct catctcgatg
//
LOCUS       DAOSGP010000007        12964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_4031_length_12964_cov_19.374235, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000007 DAOSGP010000000
VERSION     DAOSGP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12964
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4031_length_12964_cov_19.374235"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1366
                     /locus_tag="PLV49_00480"
     CDS             296..1366
                     /locus_tag="PLV49_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HOG97346.1"
                     /translation="MALRSIEITLPEGKKGQIEELLSERKEALDVRMQHIIGVWKHPV
                     KGVMYSRLSEEQILVKILVFAEESESLLKSLQERFSDEDGFRINIIPVEASLPAEEMA
                     VKSTDAADLSGDKENKSFRLSREELYEDISTATKLTRVYVVLVILSAIVAAIGLWNNS
                     VAIIIGAMVIAPLLGPNVALSLAAALGDINLAKNALKTNVVGLAIAAALSIAMGYFLA
                     VDPTIPEIESRTAVGLDEVLLALVSGCAGTLAFTSGAPATLIGVAVAVALLPPLVTSG
                     LLLGAGYTTLAYGAFLLFLVNIASINLAGVGTFLVQGISPRDKPGDRWSKQHLVFTAL
                     VIISLLVILSVTILHLWESWIG"
     gene            1439..2086
                     /locus_tag="PLV49_00485"
     CDS             1439..2086
                     /locus_tag="PLV49_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HOG97347.1"
                     /translation="MDAGDYLLYFVYVFSTIFVIVNPIEATMVYVTLTIGLPPLEKSR
                     IYKRATLVAFIIAILFAMAGDLVLRLFGITTDSLSVAGGVLLFLVAIDMLRGVHQQKK
                     VTEEELKDANSREDISVFPLATPLLTGPGAITTVVVLMGSAGSIAEKSIVILGISLTY
                     TVTLIVLKFSDYFDRVLGITGVMVLNRVMGLILGAVAVNFVAVGAWNIYQSMAGG"
     gene            complement(2100..>2539)
                     /locus_tag="PLV49_00490"
     CDS             complement(2100..>2539)
                     /locus_tag="PLV49_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HOG97348.1"
                     /translation="QNLDLSQDPGILEELTLTRRYTKSTGSYKNKQPHIQLVEKMRER
                     GGKVPTVGDRVPFVIIRGKGGKKNKTLFVDRAEDPEFVLENNISLDTEYYVNKQILPP
                     VMRIFESFGVNKDRLCSSRAQSSLFSFAGECNKPQKQKSLFDF"
     assembly_gap    2540..2639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2640..4627)
                     /locus_tag="PLV49_00495"
     CDS             complement(<2640..4627)
                     /locus_tag="PLV49_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HOG97349.1"
                     /translation="MIFQILDANYAYDSGRKPLVQLFGTTPEGQSVTVKAAGFRPYFY
                     ASAIEGCLESAADDLKAMGLEVEVLERFEPIGYQKKPKKMLQITTNDPKEVRNLRERV
                     RSLPGIRAVYETDILFKNRFLIDKSLGGMGWVEAPIPEWTLSQNAGSVQSARDLPPVD
                     AASIRPVQHEANAPLRFMSFDIECLPDHGAMPKAEKSPVILISMAFEPEYQGNKDLVL
                     VGKEIDCPRPDTRACRNEYELLNQFAAIIRDYNPDIIAGYNSNDFDFPYLQERAKQQQ
                     LDFRVGRDGSSWYIKKIVNRSDVSITGRVVVDLLPIIRSSFSLKQYTLRNVAAELLSM
                     EKYDVNPQEIEELWAEESNGEGLKRFISYSRRDSVLALRLLLDLRLMDKYIALSRASG
                     SLLQDIVNGGQSGMVENLLLRRFREISRVVPPKPDSGLSDERYVENEELKGGAVLVPE
                     KGLVEDVVILDYKSLYPTIMMAHNLCYSTVVLADSPDKKDIITSPSGGSFVSPNVSPG
                     VMPRVLRELLDQRTETKRLMKKASEEERRFLDAKQYALKILLNSFYGYSGYARARLYS
                     LALANAVTSFGRENILRTKKIIDDIGSVYMIEGRAVFKDELRLGAPTGKGFDLNVVYG
                     DTDSVFVRLSPHNEPVTLEDAGLIGKKIAETVTSSLPQP"
     gene            complement(4785..5336)
                     /locus_tag="PLV49_00500"
     CDS             complement(4785..5336)
                     /locus_tag="PLV49_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HOG97350.1"
                     /translation="MESTINIENVVASTKLAEEFDLHKIEAELEGAEYNKEKFPGLVY
                     RVKAPRAAFLIFTSGKVVCTGAKNVEDVRTVITNMAQTLKSIGFENIDLEPEIHVQNI
                     VASADLKTDLNLNAIALGLGLENIEYEPEQFPGLVYRIKTPKVVVLIFSSGKLVVTGG
                     KSPEECEEGVKIVREQLENMGLL"
     gene            complement(5479..5567)
                     /locus_tag="PLV49_00505"
     tRNA            complement(join(5479..5514,5529..5567))
                     /locus_tag="PLV49_00505"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5530..5532),aa:Thr,seq:cgt)
     gene            complement(5621..6856)
                     /locus_tag="PLV49_00510"
     CDS             complement(5621..6856)
                     /locus_tag="PLV49_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HOG97351.1"
                     /translation="MTEIADIEMEGHLIDSQMLTRVLDKIMDMGGEFEIKTFRIGEKK
                     NDTSYVQITVTGEDSAHLDRILLELNALGARLLEMEDAKTEIAPGDMVVPRGFYSTTN
                     HPTYVRYHGAWVEVVANHMDCLIVLHDGKAVCTPIGHIKKGDLVVIGTTGVKVVPPER
                     PREKSFFGFMSNEVSSERPSGELVRQLACEIIRTKNGGGKIAVVCGPALVHTGAAPSL
                     AKLIREGYVDVLLAGNAVAVHDIERQLFGTSLGMNCSGNAISGGHRNHLYAISEIMAS
                     GSIKKAIEEGKLKGGIMYEAITRGIDFILAGSIRDDGPLPEVITDTVKAKDAMGRVLQ
                     GVDMTIMLGTMLHSIAVGNLLPSYVKTICVDINPSTVTKLMDRGSAQAIGLVTDIGIF
                     LPQLAEEIQKQKKEIIRAA"
     gene            complement(6868..>7023)
                     /locus_tag="PLV49_00515"
     CDS             complement(6868..>7023)
                     /locus_tag="PLV49_00515"
                     /inference="COORDINATES: protein motif:HMM:NF037211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase"
                     /protein_id="HOG97352.1"
                     /translation="ARGFEYHLRRCGIRAIFDQPLPDDDGYTIGITKIIMSTNDKTEA
                     LLNGEMW"
     assembly_gap    7024..7123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7124..7815)
                     /locus_tag="PLV49_00520"
     CDS             complement(<7124..7815)
                     /locus_tag="PLV49_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase"
                     /protein_id="HOG97353.1"
                     /translation="MNLQRIADKLGITVDRLQSLQDTKISESSWPEPQLLRFDKEISG
                     VEAGTVIFKNGEIVWGYPSIRRPLMLEAGIKRHFIDSVAVEEKMNGHNVRITSIDGDI
                     VALTRGGFVCPYSTEVATRLLPEDIFEEKPNLVLCAEMVGPENPYVSLDVYPTDSIDL
                     YLFDVAQKNQRGTHGVHKTHEIAEDFGLKGTAFFGEFPRETAHKRIKEIVIKLGRQGR
                     EGVVLKDPENRVA"
     gene            complement(7812..8591)
                     /locus_tag="PLV49_00525"
     CDS             complement(7812..8591)
                     /locus_tag="PLV49_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG97354.1"
                     /translation="MQVKDIMTEPVTIDKSERLGHALDIMEKNDLRRLLVTNKGKIGG
                     IITTRQIARVLGARKSLGMPASSLHVASATQDSVIKVLPDMDMLDAMLLLQKTSVLVA
                     VDGEKILGWVRPREILEKANVSGLASEAMRYPLTANPNDRLVHARRTILDRDVGRLPV
                     VEAGRLVGILTEGDVAKALRAFRDLNDTASKQHTRIYNILVSDVMTHDVKYVYTDTPL
                     EEVKRIILTDNIGGLPVLNHREEVAGMITRRSILDYLVRTG"
     gene            complement(8591..9451)
                     /locus_tag="PLV49_00530"
     CDS             complement(8591..9451)
                     /locus_tag="PLV49_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG97355.1"
                     /translation="MIIKKVKDYMSTPVNVIERNEPIQRARNLMFKYSIGRLPVMDNG
                     KLVGIVTKYDITNRISQAAPEWRRRPIDKVPIQVVMTEKPITIFPDATMPQAAELMIE
                     NDISGLPVEKDGEIVGVITSRDMVRHFSEQDISSTVQELMSKNILNVHRHHTIGHVLE
                     EMNVQGISRALVYEDNRTPVGIVTRSGLTFSDIMGPKDDMETKNIKMTRKESTAGRKQ
                     NRYVRQMPFVAEDVMTSPILSLTPETNAVEASKTLAEKHIIGMPVVKDNDVVGYFSAD
                     EIIAEIGRWK"
     gene            complement(9448..10365)
                     /locus_tag="PLV49_00535"
     CDS             complement(9448..10365)
                     /locus_tag="PLV49_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG97356.1"
                     /translation="MPRAPGSGERGPLQFDSRHAKHISEVLSAASPDVVTVPPTTTIM
                     GAIKTMTFYGFSRLPIADAGTKRLLGFVTSVDVVDFLGGGLRHNLLQEKYQGNIFTAI
                     NADIREIMSSKLIYASENTSLHDVLKLMYEKNVGGLPIVDEDSRIKGIITEEDFVRSC
                     RGVDTGLVVESFMSPNVVTAPAQTTIEKMTRMIIQKGFRRMPVVQDGVLMGMVTASDI
                     MKYLGSGDAFEKVVTGDIGEVMNQPIKSLIKRSLITIEKKMDLGHAARKMMDNEIGSM
                     PVMDRGSLAGILTERDFVRALAENRGILP"
     gene            complement(10428..>10821)
                     /locus_tag="PLV49_00540"
     CDS             complement(10428..>10821)
                     /locus_tag="PLV49_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG97357.1"
                     /translation="IGEIMSTKVVTARLDEPVTKVWDRMVERDFTGLPMVRDEKPMGI
                     ITRFDILKRGWARISKESETRPVERMQLPAEKLMSSPLYSLGPTASVAEAIELMLKHD
                     VGRVSVVDEGRIMGIVDRSDLIKCYLGE"
     assembly_gap    10822..10921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11039..11593)
                     /locus_tag="PLV49_00545"
     CDS             complement(11039..11593)
                     /locus_tag="PLV49_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG97358.1"
                     /translation="METEIPVRDIMTRPVITADADLDVLSAAKRMGSANVGSLIIVSR
                     DKTTGILTERDLVRKVIAQGVDPRNLKVREIMSSPVTAIEPDASIRDAANLMLRAGVK
                     RLPVILKGKLMGIITDTDLVSGCSVGLNDILSDLLEMHRENIHFEQPRGTVSGICEVC
                     GQLSDNLENVNGELLCWSCRDGNR"
     gene            complement(11698..12180)
                     /locus_tag="PLV49_00550"
     CDS             complement(11698..12180)
                     /locus_tag="PLV49_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOG97359.1"
                     /translation="MDMILDVVVASTSRYTKFGSVRDPDRAEDLSGKVILEKIRTAGY
                     TGNYLLIPDGVEPIQRAIKTSSADAMVICGGTGLTALDLTIEAAEPLFEKTLPGFGEI
                     FRWQSMKEVGTRAMLTRATAGVFMKKPIFCLPGSPGAARLGMDLILAEIEHILKHVME
                     "
     gene            12264..>12964
                     /locus_tag="PLV49_00555"
     CDS             12264..>12964
                     /locus_tag="PLV49_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HOG97360.1"
                     /translation="MTLLAVDVGAGTQDILLYQEGVPIEGSTKMVLPSQTMIVGNRIS
                     RARMAGRDIFLHGPIMGGGASTMAVRWHLAAGLRVFATPSAAATINNDLGQVEALGVI
                     IQEDAPASAEIVETGDIDIPSLQRAFQLFDISLPKDIAVAVQDHGYSPGKSNRQVRFE
                     HMVAAIRSGGSLDDFAFKEYPQEMTRMVAVRDTLIKEGFEPFIMDTGPAAIFGAALDP
                     RMEDPALIINFGNSHT"
BASE COUNT         2662 a         3616 c         3144 g         3242 t
ORIGIN      
        1 agaccagtat gatctctcct ggggcagcag aatctatcgc cttctgaatt cggtcgcctg
       61 aatggacggt gatatcacca tttgcagggc ccgtgaacag gatgaacgca agcgatactg
      121 ccaataaaat ggatcccttt tctgccatgg ccttcacacc gccgatgaac tgattttttg
      181 agtataaaac gccatccctc acgcacgctt cctatttggc ccaacaaggc agattttata
      241 agcaagatgc ctctattaaa acaggaggac aagaaggcgg ctgctggagt gaataatggc
      301 cctgcgttca attgagataa cccttcctga agggaagaaa gggcagatcg aagagctgct
      361 cagcgaaaga aaggaagctt tggacgttag aatgcagcat atcatagggg tctggaagca
      421 tccggtaaag ggtgtgatgt acagccgcct ctcagaagaa caaatcctgg tgaagatcct
      481 cgtctttgcc gaggagagcg agagtctgct caagtccctg caggagaggt tctcggacga
      541 ggatggtttc cggataaata taatccctgt cgaggcctcc cttccagcgg aagagatggc
      601 agtgaaatcc actgacgccg ctgacctctc cggggataag gagaacaaga gcttccggct
      661 gagcagggag gagctttacg aggatatctc cactgccaca aagctgaccc gtgtctatgt
      721 ggttctggtg atcctctctg ctatagttgc tgccattggt ttatggaata acagcgtagc
      781 aataataatc ggtgccatgg tcatagcccc tttgctgggt cccaatgtcg ccttatcttt
      841 agccgccgca ctgggtgata tcaatctggc caagaatgca ttaaagacga atgtggttgg
      901 tctggccatt gccgccgccc tttccattgc aatgggctat tttttggccg tcgatcccac
      961 cattcctgag atcgagtcca gaacggccgt gggcctggac gaggtcctcc tcgccctggt
     1021 ctcaggctgc gcgggaacgc tggccttcac ctctggcgcc ccggcaactc tgatcggagt
     1081 agcagtggca gttgccctcc tccccccgct ggtgacctcc ggcctgctct taggggctgg
     1141 atacaccact ctagcttatg gagcgttcct cctcttcctg gtgaacatcg ccagcatcaa
     1201 cctggcaggg gttggcacat ttctggtgca gggcatctct ccccgggata agccagggga
     1261 cagatggtca aaacagcatc tggtcttcac tgccctggtt atcatcagcc ttttggtcat
     1321 actctccgta accatccttc acctctggga gagctggatt ggatgagatg atctatttaa
     1381 ggcaagggcc acatatgagc tataggccgc agaacaaaat ggccaaaagg gaaattcaat
     1441 ggatgcaggc gattacttac tctacttcgt atatgtcttc agcaccatct tcgtgatagt
     1501 caatcccatc gaggccacca tggtctatgt caccctgacc atcggcctgc cccctctgga
     1561 aaagagccgt atctataaga gggctacact ggtcgccttt atcattgcca ttctcttcgc
     1621 catggcaggc gatctcgttc tccgcctctt cggcatcacc acggacagcc tatcagtggc
     1681 tggaggagtt ctcctcttcc tggtggccat agacatgctc agaggagtcc accagcagaa
     1741 gaaggtaacg gaagaggaat tgaaggatgc caatagcagg gaggatatct ccgtctttcc
     1801 cctggccacg ccccttctca ccggaccggg tgcgatcacc acggtggtgg tcctgatggg
     1861 atcggcagga agcatcgcag agaagtcgat cgtcatcctg ggaatatccc tgacctatac
     1921 tgtgaccctg atcgtcctca agttctccga ctacttcgat cgggtgctgg gcatcaccgg
     1981 ggtaatggta ttgaaccgag taatgggcct gatcctggga gcagtggcgg tgaacttcgt
     2041 ggctgttgga gcctggaaca tctatcagtc aatggctggt ggctgaaggc caaacctccc
     2101 taaaagtcga agagagactt ctgcttctgt ggcttgttgc attcgccggc aaagctgaac
     2161 aggctgctct gcgcccggct ggagcagagc cggtccttgt tcaccccgaa gctctcgaat
     2221 atgcgcatca ccggcggcag gatctgcttg ttcacatagt actccgtatc cagagagata
     2281 ttgttctcca gcacgaattc cgggtcctcc gcccggtcca cgaaaagggt cttgttcttt
     2341 ttgccgccct tgccccggat gatcacaaag ggcactctgt cgccgacagt gggaaccttg
     2401 ccgcccctct ctctcatctt ctccaccagc tggatgtgag gctgcttgtt cttgtacgag
     2461 cccgtgctct tggtgtacct cctggtgagg gtcagctcct ccagtattcc cggatcctgg
     2521 gataggtcca ggttctgcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     2641 gctggggaag gctggatgtg acagtctcgg cgatcttctt tccgatcagg cccgcatcct
     2701 cgagcgtcac cggctcatta tggggactga gacggacgaa gacgctgtca gtatccccat
     2761 agaccacatt gaggtcgaat ccctttcctg tcggggctcc tagcctcagt tcgtccttga
     2821 agactgccct gccttcgatc atatagaccg atcctatatc gtctatgatc ttcttcgtcc
     2881 ggagaatgtt ctcccggcca aaggaggtga ccgcattggc caaagccagg ctgtacagcc
     2941 tcgcccgggc gtaacccgag tagccataga agctgttcaa cagtatcttc agtgcatact
     3001 gcttggcatc taaaaatcgc ctctcctctt cgctggcctt cttcatcaac ctcttggtct
     3061 ccgtcctctg atcgagcaat tcccttaaga cccggggcat tacccccggt gaaacattcg
     3121 gggagacgaa acttcctccg gaaggcgagg ttatgatatc ttttttgtcg ggagagtcag
     3181 ccagcaccac cgtcgagtag cagaggttat gagccatcat aatggtggga tatagcgact
     3241 tgtagtctaa aatgaccaca tcctccacca gccccttctc aggcaccagc actgctcctc
     3301 ctttcagctc ttcgttctcc acatatcgct cgtccgagag accggaatct ggcttggggg
     3361 gcaccacccg ggatatctcc cggaagcggc gcaggagcag gttctcaacc atccccgact
     3421 gtccgccgtt gactatgtcc tgcaagagcg agccgctggc ccgtgagagg gcgatgtact
     3481 tgtccatcag ccgaaggtcc aatagcagac gaagggcgag gacagagtct cggcgggagt
     3541 agctgataaa ccgcttcaga ccttctccat tgctctcttc tgcccagagc tcctcgatct
     3601 cctggggatt cacatcgtac ttctccatcg aaagaagctc cgcagcaaca ttcctcaggg
     3661 tatactgctt caggctgaac gaggagcgta tgatgggcaa tagatcgacc accacccggc
     3721 cggtgatgga gacatcgctt cggttcacta tcttttttat gtaccaggag ctgccatccc
     3781 ttcctaccct gaaatcgagc tgctgctgct ttgccctctc ctgaagataa ggaaaatcga
     3841 aatcattgga gttgtagcca gcgatgatat cgggattgta gtccctgatt atggctgcaa
     3901 actgatttag aagctcatac tcgttcctgc aggctctggt gtccggacgg gggcagtcga
     3961 tctcctttcc taccagtacc aggtctttat tgccctgata ctccggctcg aaggccatgc
     4021 tgatcagtat caccggggat ttctctgcct tgggcatggc accatgatcc ggaaggcact
     4081 cgatgtcaaa gctcatgaaa cgcaagggtg cattggcctc atgctgcacc ggacggatgg
     4141 aggcagcatc caccggaggc aagtctcttg ctgattgcac tgatccggca ttctggctca
     4201 aggtccattc cggtattgga gcctcgaccc atcccattcc tcccagcgat ttgtcgatca
     4261 gaaatctgtt cttgaagagg atatccgtct cgtagaccgc ccggatgccg ggaagggagc
     4321 gcaccctctc cctcaggttt cttacctctt tgggatcgtt agtggtgatc tggagcatct
     4381 ttttgggctt cttctgatag cctatgggct cgaagcgctc cagcacttcc acctccagac
     4441 ccatggcctt caggtcgtcc gccgcacttt ccagacagcc ttctattgcg ctggcataaa
     4501 aatagggcct gaatcctgct gccttgactg tgacgctctg cccttctgga gtggtgccaa
     4561 atagctgcac caagggcttt cgccctgaat catatgcgta gttagcgtcc aggatctgga
     4621 agatcatcgc ttataaatgg aaacttttgt ttatataact aatcatggcc aggggcaaaa
     4681 gaggcggaaa aaatgcccat atttcccagt atttgcagaa gtctggagac aaaaaaggga
     4741 ttgatccctg ctttcaatag atctcatcct tctgggcaat tcagttatag cagccccata
     4801 ttctccagct gttccctcac gatcttgact ccctcttcgc actcctctgg cgacttgcca
     4861 ccagtgacca ccaattttcc ggaactaaat atgagcacta ccacctttgg ggtttttatc
     4921 cggtagacca ggccgggaaa ctgctccggc tcgtattcga tattctccag tcccaggccg
     4981 agggctatgg cattgaggtt caggtctgtc ttcaaatctg cagatgccac aatattctga
     5041 acgtggatct ccggctccag atcgatattc tcgaagccta tggacttgag agtctgggcc
     5101 atattggtta taaccgtgcg aacgtcctcg acgttcttgg cgccggtgca taccactttg
     5161 ccggaggtga atatcagaaa agcggctctg ggggctttta cccggtatac caggccggga
     5221 aacttctctt tattgtattc tgcgccctcc agctcagcct cgatcttatg gagatcgaac
     5281 tcctcagcca gcttggtgga tgccaccacg ttctctatgt ttattgtgga ctccatactc
     5341 aagtatctcc agttcatact tacaaactgg gggtatatat atgtgagtat ttaagattta
     5401 taaagcgctt ataaactgat tatatactgc ttataaacca ccggcaaatc ccaagatcgg
     5461 caggatttaa aaataaaaag ccgccgatgg gattcgaacc catgaactgc tgatctttcg
     5521 gaccatctta cgaatcagcc gctttaaccg ggctaagcca cggcggccct ctgggactct
     5581 actagcaggg gcatatgcga ataaaagatt tgcgatccag tcaggcagct cttatgatct
     5641 cttttttctg cttctggatc tcttcagcca gctggggcaa gaatatgcca atatcggtca
     5701 caaggcctat ggcctgggcc gatcccctat ccatcagctt tgtcactgtg gagggattga
     5761 tgtcaacaca gatggtcttg acatatgagg gcaagagatt gcctacagca atggaatgga
     5821 gcattgtgcc cagcattatg gtcatgtcca ccccctgcag aaccctgccc atggcatcct
     5881 tggccttgac ggtgtcggtt atgacctcgg gaaggggtcc atcatcgcga atggagcccg
     5941 ccaggatgaa gtcgatccct ctggtgatcg cctcatacat tattccaccc ttgagcttgc
     6001 cctcctctat ggccttcttg atggagccgc tggccatgat ctcgcttatg gcgtacagat
     6061 ggtttctgtg tcctcctgat atggcatttc ccgagcagtt catgcccagg ctggtgccaa
     6121 agagctggcg ctcgatatca tgtaccgcta ccgcatttcc tgccaggagg acatccacat
     6181 atccctctcg gatgagcttg gccagggagg gggcggcacc ggtatgaacc aacgctggtc
     6241 cgcagaccac agcaatcttt cctcctccat tcttggtcct gatgatctca caggccagct
     6301 ggcggacgag ctctccgctg ggcctctctg aggagacctc attggacatg aatccgaaga
     6361 agctcttctc cctcgggcgc tcgggcggta ccaccttgac ccctgtggtt ccaatcacca
     6421 caaggtcccc cttctttata tggccgatgg gcgtgcatac cgcttttccg tcatgcagga
     6481 cgatgaggca gtccatgtgg ttcgccacaa cctccaccca ggccccatga tagcgcacat
     6541 aggtgggatg attggtggtg gagtagaatc cccgggggac taccatatct ccaggagcga
     6601 tctcggtttt ggcgtcctcc atctccagga ggcgcgctcc cagggcattc agctccagga
     6661 gtatcctgtc caggtgagct gaatcctctc cggtcaccgt gatctgcaca tagctggtat
     6721 cgttcttctt ctcccctatc cggaaggttt tgatctcaaa ctctcctccc atgtccatga
     6781 tcttgtccag gactcgtgtt agcatctggg agtctatgag atggccttcc atctcaatgt
     6841 cagcaatctc tgtcataagc tctgccttca ccacatctct ccattcaaga gggcttcagt
     6901 cttatcattt gtgctcatga ttattttggt gattcctatg gtatagccgt catcatcagg
     6961 cagcggctgg tcgaatattg cccggatccc gcatctgcgc aggtgatact caaatccccg
     7021 ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngctaccc tgttttccgg
     7141 gtccttcaag accaccccct cccttccttg ccggcccagc ttgatcacta tctctttgat
     7201 ccttttgtgc gccgtctccc ggggaaattc tccaaaaaaa gcggtccctt tcaggccgaa
     7261 atcctctgct atctcgtggg tcttatgcac cccatgggtc cctcgctggt tcttttgggc
     7321 aacatcgaag aggtataaat cgatgctgtc agtgggatag acatctaaag atacataggg
     7381 gttctccggg cccaccatct ccgcgcaaag gaccagattg ggcttctctt cgaaaatatc
     7441 ctccggcagg agccttgtcg ccacctctgt ggagtaaggg cagacaaaac cacctctggt
     7501 cagggccacg atatccccgt ctatggatgt tatgcggaca ttgtggccat tcatcttctc
     7561 ctccactgcc acagaatcga taaaatgacg ttttatcccc gcttcaagca tcagaggacg
     7621 gcggatgctg gggtagcccc agacaatttc tccgttttta aaaatcaccg tcccggcctc
     7681 aacccctgag atctccttat caaatctcag cagttgaggc tcgggccagc ttgattcaga
     7741 tatcttcgta tcttgcaggc tttgcagccg atctacagtt attcccagtt tatctgcaat
     7801 cctttgcaga ttcatccagt cctcaccaga taatccagta tggacctcct ggtgatcatg
     7861 cccgctacct cttctctgtg gttgagcacg ggcaggccgc ctatgttatc cgtgagaatt
     7921 attctcttga cctcctccag aggggtatcg gtgtagacgt acttgacatc atgggtcatc
     7981 acatcagaga ccagtatgtt gtagatgcga gtatgctgct tgctggcggt gtcattgaga
     8041 tccctgaagg ctcgcagggc tttggccaca tctccctctg ttaatatccc caccagacgt
     8101 cctgcttcga ccacgggaag ccttcccaca tctctgtcca gaattgtgcg ccgggcatga
     8161 accagacgat cgttggggtt cgcggtgagg ggatacctca tggcctcgga ggccaggccg
     8221 ctcacattgg ccttctccag gatctccctg ggcctgaccc agcccaggat tttctcgccg
     8281 tccactgcca ccagcaccga tgtcttctgt aagaggagca tggcatctaa catgtccatg
     8341 tccggcagga ccttgataac gctatcctga gtggctgatg ccacatgcag agaggaggcc
     8401 ggcatgccca gggattttcg ggcaccaaga acgcgggcaa tctgtctggt agtgattatt
     8461 ccgcctatct tgcctttatt ggtcaccaga agcctgcgca gatcgttctt ctccattata
     8521 tccagcgcat ggcccaaccg ttccgattta tcgatagtaa cgggctctgt cataatgtct
     8581 ttaacctgca tcacttccac ctgccaatct ccgctatgat ctcatcggct gagaaatagc
     8641 ccaccacatc attgtccttt accaccggca tgccgatgat atgcttttct gcaagagtct
     8701 tgctggcctc gaccgcattg gtctccgggg tcaggctgag tatgggcgag gtcatgacat
     8761 cctcggccac gaagggcatc tgccttacat atcgattctg ttttctgccc gcagtgctct
     8821 ccttccgggt catcttgatg ttcttggtct ccatatcatc cttcggcccc atgatatcag
     8881 agaacgtaag gcctgagcgg gtcactatgc ctacaggagt cctgttatcc tcatagacca
     8941 gggcccggct gatcccctgc acattcatct cctccagaac atggcctatg gtatggtggc
     9001 ggtgaacatt gaggatgttc ttgctcatca gctcctgcac agttgaggat atatcctgct
     9061 cggagaagtg cctcaccata tccctggagg tgatcacgcc cacgatctct ccgtcctttt
     9121 ccaccggcag gccgcttata tcgttctcga tcatcagttc cgctgcctgg ggcatggtgg
     9181 catcagggaa tatggttatc ggcttctcgg tcatgaccac ctggattggg accttgtcta
     9241 tgggcctcct cctccattct ggggctgcct ggctgatccg gttggtgata tcgtatttgg
     9301 tgactatgcc caccagcttg ccgttatcca taaccggaag cctgccgatg gagtacttga
     9361 acatcaggtt ccttgctctc tgaatgggct cgttccgctc gatgacattt acgggggtgc
     9421 tcatgtagtc tttgactttc tttatgatca tggcagtatt cctctgttct cagctaaggc
     9481 cctcacgaaa tctctttctg tcagaatgcc tgctaatgaa cccctgtcca ttacgggcat
     9541 cgatccaatc tcgttgtcca tcatctttct ggccgcatgg cccaggtcca tcttcttctc
     9601 gatggtgatt aagcttctct tgatcaggct ctttatgggc tggttcatca cctcgccgat
     9661 atcgcccgta accacctttt cgaatgcatc accgctgccc aggtatttca ttatgtccga
     9721 tgcggtcacc atgcccatga gaacgccatc ctgcaccaca ggcatccttc ggaagccctt
     9781 ctggatgatc atcctggtca tcttttcgat ggtggtctga gcgggtgcag ttacaacatt
     9841 ggggctcatg aaggattcaa ccacaaggcc cgtatccacc cctcgacaag agcgcacgaa
     9901 gtcctcctcg gtgattattc ccttgatgcg ggaatcctca tccactatcg gaaggccgcc
     9961 cacgttcttc tcgtacatga gtttcaagac atcatgcaat gaggtgtttt cgctggcata
    10021 gatgagcttg ctgctcatta tctcccttat atctgcatta atggcggtga agatattccc
    10081 ctgatacttc tcctgcagaa ggttgtgccg aagccctccg ccaaggaagt ccactacatc
    10141 caccgatgtc acaaagccaa gcagcctttt tgtgcctgca tctgcgatgg gaagcctgga
    10201 gaagccgtag aaggtcatgg tctttatggc ccccatgatg gtggtagtag gcgggactgt
    10261 gaccacgtcc ggcgatgctg cgctcagcac ctcgctgatg tgtttggcat gcctggagtc
    10321 gaactgcagc ggtcccctct ctcctgagcc cggagctctt ggcattctat cgttcctctg
    10381 cggtatcctc gttatctcct ttccactagt tggaccattg ctcattttca ctcccccagg
    10441 taacatttga tcagatcgct tctatccact atccccataa tccttccctc gtctaccacc
    10501 gataccctgc ccacatcgtg cttgagcatc agctctatgg cctcggcaac gctggccgtc
    10561 ggcccaaggc tgtagagagg ggagctcatc agcttctcag ccggcaattg cattctttcc
    10621 accggtctgg tctcgctctc tttgctgatc ctggcccagc ctctcttcag gatatcgaat
    10681 ctcgtgataa ttcccatggg tttctcatcc ctcaccatag gcagaccggt aaaatctcgt
    10741 tccaccatcc ggtcccagac cttggtcacc ggctcatcca ggcgggcggt taccaccttt
    10801 gtgctcatga tctcgccaat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 ngtggagtcc tccacataaa gaggatctct agagaaaatg tcttcgacta gcattccgac
    10981 tcccctgctg tcccgcgatt gccatattgg aatttgccat atcgcagtta ttctaaggct
    11041 atcgatttcc gtccctgcag ctccagcaga gcaactcgcc attcacgttt tccagattat
    11101 ctgatagctg gccgcagact tcgcagattc cgctcacagt tcctcggggc tgctcgaaat
    11161 gaatgttctc ccggtgcatc tccaacagat cgcttaagat atcattcaat ccgacggaac
    11221 agccagagac caggtcggta tctgtgatta tgcccatcag cttgcccttg aggataacag
    11281 gcagccgttt tactcctgcc cggagcatga ggttggctgc atctcttatg cttgcatcgg
    11341 gttcgattgc agtgacaggt gagctcatga tctccctcac cttcagattt ctgggatcaa
    11401 ccccttgggc tatgaccttc ctgaccaggt ccctttccgt taaaatgcct gtggttttgt
    11461 ctcgggatac aattatcaga cttcccacat tggccgagcc catccttttg gcagcgctca
    11521 atacatctag atcggcgtca gcggtgatca ccggtcgggt catgatatct cgcactggaa
    11581 tctctgtctc cattggccaa gtctccgtga ttagctctat gtgcttatta gtgtactaat
    11641 gatataaaag gctactccgg ggcaatagtt tttgattcga ttaatattat ttttccccta
    11701 ctccatcaca tgtttcagga tatgctcgat ctctgctaat atgaggtcca ttcccaagcg
    11761 agctgccccg ggagagcctg gcaagcagaa tattggtttt ttcataaaga ctccggctgt
    11821 tgcccgggtt agcatggccc gggttccaac ctccttcatg ctctgccaac gaaagatctc
    11881 cccaaacccc gggagcgtct tttcaaagag cggctctgca gcctctatgg ttaggtccaa
    11941 agcggtgagt cctgtgccgc cgcagatcac catggcgtca gcgctgctcg tcttaattgc
    12001 acgctggatg ggctctacgc cgtccgggat gagcaggtag ttgcctgtat accctgccgt
    12061 ccttatcttc tccagtatca ccttcccgga caggtcctct gccctatccg gatcccgaac
    12121 cgagccgaac ttagtatagc gagacgtgct cgcgaccacc acatctaaga tcatgtccat
    12181 tcctctctgg atgaggataa aatttttccc gtagcccatc caattgggac cagacaaaag
    12241 ctctctaggg gaaggaatga tctatgacgc tcttagcggt tgatgttggg gcagggactc
    12301 aagacatcct gctttaccag gagggggtgc ccatcgaggg ctcgaccaag atggttctgc
    12361 ccagtcagac catgattgtt ggaaacagga ttagccgggc caggatggcg ggacgggaca
    12421 tttttttgca tggccccatc atggggggcg gagcctccac catggcagtg aggtggcacc
    12481 tggcagcggg actgagggtt tttgccaccc cctcagccgc agccaccatt aataatgacc
    12541 tgggccaggt tgaggccttg ggagttatca tccaggagga tgcaccggca tcagcagaaa
    12601 tcgtagagac gggagacatc gacattccct ctcttcagcg ggctttccag ctattcgaca
    12661 tatctctgcc caaggatatt gccgtggcgg tgcaggacca cggctactct cccggcaagt
    12721 caaaccgcca ggtccggttc gagcatatgg tcgcagctat ccgttcgggc ggaagcctgg
    12781 atgatttcgc ttttaaagag tacccacagg agatgaccag gatggtggca gtgcgggata
    12841 ccttgataaa agagggattt gagcccttta taatggacac cggtcctgca gccatcttcg
    12901 gtgccgccct cgacccgcgg atggaggacc cggcattgat catcaacttt ggaaacagcc
    12961 atac
//
LOCUS       DAOSGP010000008        12580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_4197_length_12580_cov_18.651497, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000008 DAOSGP010000000
VERSION     DAOSGP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12580
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4197_length_12580_cov_18.651497"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1144
                     /locus_tag="PLV49_00560"
     CDS             41..1144
                     /locus_tag="PLV49_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HOG97361.1"
                     /translation="MDVLVLCIDRDNDLGRKGGVITPVVGREANIEAALKLGMSDPED
                     SDVNTIFGGIKTFDEIAASGRDVEIASIAGDLKVGLISDSKLASQLEDLLARLHPQGV
                     IVVSDGAEDEAILPIITSRIKVEGVRRILVKQSPNLESTYYLLKQLFTDPKLSHTIFI
                     PPALALLMFAFFSLIGYPSWAIVAITGSVGLYLLFRGLGLDDSMDEAKQTLKKSLYAR
                     KIAFITYILAAMLVVIATVNGVAYFWQYFHEPTFPGYFTLIMIYLYESIWWYMAAALC
                     VNLGKVMDMYMEGIRDARTISFLFFILATGLVIWSTSSFILASNADLEFGIGMVESVQ
                     KLAISAIGAILITVIGINVSKRIATDNAFLPSK"
     gene            1152..2042
                     /locus_tag="PLV49_00565"
     CDS             1152..2042
                     /locus_tag="PLV49_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tributyrin esterase"
                     /protein_id="HOG97362.1"
                     /translation="MLQILASGEKCLGDFTFDFYWQHQGSRLDMDQKIGEASLRQIVQ
                     SLRKGERVNISGDVGSRLGSSLGVDLIRLGGKGGPIEGTGSIVVEGDVGRRMGISMLR
                     GAIYVSGRVSEPMGNVIEVDSDLTGYRMFVSITEALEKGWDVTEPNHLDQQGLFIEDG
                     IIRDTLGARNPADKTIKLEGDAGMSTGILMRSGQIIVEGCAQENTAVLLRGGRILVRG
                     STKDFTGAEMRGGEVFIEGDAGSFTCARMKGGVVYARQALPLPPAKRHPLSPSERTVV
                     ARALELGPMQALMYSRFGLQ"
     gene            complement(2036..3145)
                     /locus_tag="PLV49_00570"
     CDS             complement(2036..3145)
                     /locus_tag="PLV49_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG97363.1"
                     /translation="MLNVLILGVGQCGNRILDAVNKEAMGGGGASKIAKYCLKPKFPS
                     RVETVAINTAINDLKELRYTTAKDRLHVPNLHGMGANRNVGKQAFMDNRDSIMGEIEK
                     RGDFDLAFVLTSTSGGTGSSFSPLLINELKRQYPNITVVTVAILPFREEGSIYLQNAA
                     FSMRELMELDADGIILADNQYMKRFSGDIASAYDKINSTIAKRLLFLIESLDSEMLSV
                     TDLGDFKTVMNGGLRIGTMGYYQADGKNNSVKDAIENSLKPNNLLYPANVADDAARAM
                     VIIQGSKEHLDVDQITKEVEKITASAGHVFKGIVVKKGHPRVLSVFTLAAAPELESIY
                     AQAARGMQDEKEKRSRARKQLDDAFAHIEDLEEIY"
     gene            complement(3177..4442)
                     /locus_tag="PLV49_00575"
     CDS             complement(3177..4442)
                     /locus_tag="PLV49_00575"
                     /inference="COORDINATES: protein motif:HMM:NF017561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97364.1"
                     /translation="MKSGERREKSLKRTIDLQDRDMGKKQHLDILRLVCLAVLILLLS
                     TAFIGAVQGADDDDDDEVTYNDFTLGIGDRIDISNYRAELIEIQSVKDGIAVLQVSRT
                     GGGLDEQRAFLENRANNFAGGAEKGGITLTVVDILDDESARVRIEYQESLGTPSRRAS
                     ERPVSSGDKPALTVQKSFDKSEMSVGDEVKVTVTIMNKGTGKAQNIKLDDAPPLPEFS
                     YVAGYPPKIKDTLDPGQSDSAVYMMNAIKEGTIRVSAIQVSYTDSKDNVLLNSSEPFD
                     TVINPKSVADLAIRMEVPGPLPLGGTGMANISIENVGKAPATRIEAKGDIKPPVGLEA
                     SGFERSFFEIPPGGEVNYSVMLSGKDSGNYTIHLKTTFDGGDGSAIREGSAEVVVLKR
                     EYKYEYLLLLIPIIIIAAWLYRRHKEYKY"
     gene            complement(4408..5256)
                     /gene="nadC"
                     /locus_tag="PLV49_00580"
     CDS             complement(4408..5256)
                     /gene="nadC"
                     /locus_tag="PLV49_00580"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOG97365.1"
                     /translation="MLRSELERFIGEDLGEWDDSSTLIPELTACGIIIAKEDCIISGL
                     AEAVEIFQYFGLKSELLFDDGEFVPAMSTVLEVVGSARKILQSERLVLNFMARMSGIS
                     TLTRECVLLAGGVRVAATRKTTPGFRLFEKRAVYLGGGDTHRFNLSDAILIKDNHIMI
                     LGLKECLRRAKDKSSFTKKIEVEIESLEDMLIAAREGADIIMFDNMDPAKIAEGVDVL
                     KKEGLRERVILEASGGITLDNIKDYGRSGVDVISLGALTKNARWIDLSLEIENDEEWR
                     AKREIA"
     gene            5297..6109
                     /locus_tag="PLV49_00585"
     CDS             5297..6109
                     /locus_tag="PLV49_00585"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HOG97366.1"
                     /translation="MPLKVGLVGCGAIGAEIAKAIDSGVVDAELIAVCDHNPQTALAL
                     IESLQHKTKKADLAELVRLSDVVVEAASQKAVPAVARAALAAGKRLMIMSVGALADMD
                     LFREIKSLAKSHCSQVYIPSGAISGLDALKSARAGRIERVTLTTTKNPSGLRGAPYIM
                     ENRIDLDSLSGPTQIFSGSAIEAVRAFPANVNVAATLYLAAGEKEVLVKVFADPNIRV
                     NRHEIEVEGDFGRFFTRVENVPSPRNPKTSYLAALSAISTLRSIVEPIKIGT"
     gene            complement(6121..7242)
                     /locus_tag="PLV49_00590"
     CDS             complement(6121..7242)
                     /locus_tag="PLV49_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HOG97367.1"
                     /translation="MIYLYYSEKFQGYNFGPEHPFNPARLMLTFSLMEESGLIDEKVC
                     RLEPRPASEEDILRVHTPEYLAAVKLEEPDLAFGLGSDDTPVFAGICESSRMLAGASI
                     DAARRIIAEDCSAFNIGGGLHHALPTVASGFCVFDDPALAIRVLRDRFDRVLYIDIDG
                     HHGDGVQQIFYTDPSVLTISMHESGLYLFPGTGFIEETGDGEGKGYSVNIPMPMYSGD
                     EQYLRAFDQIVPILFEWFRPQAVVAQLGIDTHYSDPLTSLNVTLNGYTGLVRRIVDLA
                     RQHSGSRLLALGGGGYNMEVVPTAWTSVLHIMRGEPLPEYLPPYWVELFMNLTGHEPL
                     SNPDIEMRVGTNTENRINKELSGTLQGLKEKMNEVHGLF"
     gene            complement(7239..7436)
                     /locus_tag="PLV49_00595"
     CDS             complement(7239..7436)
                     /locus_tag="PLV49_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97368.1"
                     /translation="MADEDHAVLEHFIPFCPKCGNECEHKDLRRALDADEWKKIVIET
                     IYYCKNCDNYWSDGLIEKGYK"
     gene            complement(7513..8361)
                     /locus_tag="PLV49_00600"
     CDS             complement(7513..8361)
                     /locus_tag="PLV49_00600"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HOG97369.1"
                     /translation="MLSFLVAVQLIALAITPAISATDNRVFEDPASISNPIIYMLLIL
                     IFTAILLLAIKLKGDWLVRGFILISIALTIFYVISAIMPMWLALLPTLAVMLLLHYYP
                     EWYILDAFGILVCAGVTSLLGVSMTVMPVIILMVILAVYDAISVYKTRHMVALAEGAI
                     KMKAPLLFVVPKSRDYSFRRKDAVSISMGNDKKKGDRGAYFLGMGDAIIPSILVISAS
                     WSYPPGDILGLNLPALGAMLGTYAGFILLMTTSRDRPQAGLPFLNSGSILGFLAGCLA
                     AGIQPF"
     gene            complement(8424..8948)
                     /gene="cyaB"
                     /locus_tag="PLV49_00605"
     CDS             complement(8424..8948)
                     /gene="cyaB"
                     /locus_tag="PLV49_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOG97370.1"
                     /translation="MIEVEVKARAREDTKERIAALGATPIGVENHLDLYFNSPLRDFK
                     ESDEALRIRIKEDGARLTYKGPKLDQQTKSRRELTIRIDDPTQMRDVLSLLGFVLSAK
                     VRKKRTKYSYQGMVIALDEVEGLGTFLEVEAQAEANWENEKDRVLSVLKRLELEDTIR
                     RSYIELLEEKRADL"
     gene            complement(8945..11521)
                     /locus_tag="PLV49_00610"
     CDS             complement(8945..11521)
                     /locus_tag="PLV49_00610"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOG97371.1"
                     /translation="MSELSKEYNFKQVEEKWERSWDSSIYYFDWESKKPQYIIDTPPP
                     YPTGNFHIGNALNWCYIDFAARYKRMRGYNVMFPQGWDCHGLPTEVKVEQLNHITKNQ
                     VPREEFRRLCEKLTEEAIDRFHKSMGRLGLSIDWSNEYATMKPEYYVKTQTSFVRMYQ
                     KGQIYREDHPVNWCPRCGTAIALAEVEYDSRTSTLNYMHFCAEDGEIEIATSRPELLP
                     ACVAVAVHPEDQRYQKYIGKTVKVPLFNYSVPVLADPAVDSSFGSGFVMICTFGDKQD
                     VRWWMEHHLPLRQAIDRGGRMMPIAGPYSGLTVEETKEKITQDLTEQGIIFKQEPLEQ
                     NVGLCWRCKTPIEILSEQQWFVRIDAPEIKKVAEEIEWIPPHMRVRLENWADSMEWDW
                     CISRQRIFATPIPAWYCRHCHEALVAEESWLPLDPNLTQPKKKCKCGSSEFIPEQDVL
                     DTWMDSSISALAVAGWPDRTDLRMPTQLRPQGHDIIRTWAFYTILRTGALEGIRPWDA
                     ILINGMALGEDGHKMSKSLNNFIRPEEVFESNGADALRQWAAIGGSPGNDIPFQWKEI
                     TAASRFQQKLWSIFRFSLPLIAPADAAKGQVDRWLSAELDQLITKTTEAMDRFQFDEA
                     LKAIRAFAWDVLADNYIELVKARLYGQDGPEKRAAQDTLFTALETLARLMAPFTPFLS
                     EEIYHTLTGQSVHVQSWPVARGQPMDRAGLAIKEVASTLRRYKAEKGVALNSPLPGIV
                     VYSDLDLETADLAGVANSPVESRTGKPSLEMKPIAVKPQMKVIGPRFRDRSQRIIMAL
                     MGMDPADIARQKEAGSIAVDLDGEILELPPEAAEVEIQTLSAGEAVDILKTKEATVLV
                     RR"
     gene            11597..12088
                     /locus_tag="PLV49_00615"
     CDS             11597..12088
                     /locus_tag="PLV49_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HOG97372.1"
                     /translation="MELVIYHANQCDPRKCSGKKLARFDLVRLTHHISQLRPYIVLSP
                     FSEKALSPEDKGARGLAALDCSWAHAEEVFARFKLQERALPFLVAANPVNWGKPFKLS
                     TVEALAAGLVILGEKSQAELILSKFNWGHVFLELNREPLSEYAAARDSAEVVRIQNEY
                     MSE"
     gene            12128..>12580
                     /locus_tag="PLV49_00620"
     CDS             12128..>12580
                     /locus_tag="PLV49_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOG97373.1"
                     /translation="MTAGLIPAEELRKLQFRMNKLMDELGLDALGSKYIDELGRMQKR
                     MGDLMEEVEGPNLKKGVIRPLADVHETEEALVVTMDMPGVEKQDINISVVEDELQISA
                     QRKSETEANEQDYHRRERTFTRFERRVLLPESIKTEEARATLANGVLQI"
BASE COUNT         2694 a         3455 c         3449 g         2982 t
ORIGIN      
        1 cacagaatcc tttaagtaca atcttttcaa atagtagacc atggatgtcc ttgtgctctg
       61 catagatcgg gacaatgacc tgggccgcaa gggtggggtg ataacccccg tcgtgggcag
      121 agaggccaat atagaggccg ccctgaagtt gggcatgtct gacccggagg actcggacgt
      181 caacaccata ttcggaggca tcaaaacctt tgatgagatt gctgcatcag gcagggatgt
      241 agagatcgcc tctattgcag gagacctgaa ggtcggcctg atctcagaca gcaaacttgc
      301 ctctcagctg gaggatctgt tggcgcgctt gcatcctcaa ggagtgattg tggtctctga
      361 cggggcggag gacgaggcca tcctgcccat catcacctcc cggatcaagg tagaaggagt
      421 gcgccggatt ctggtcaagc agagccccaa cctggagagc acctactacc ttttaaagca
      481 gctcttcacc gaccccaagc tcagccatac catattcatt cctcctgctt tggctctgct
      541 catgttcgca ttcttcagcc tgatcggcta cccaagctgg gcgatagtgg ccatcacagg
      601 ctcggtgggt ctgtatctct tattccgggg gctgggcctg gacgactcca tggatgaggc
      661 aaagcagacg ctcaagaaat ccctttatgc ccggaagata gcctttataa cctatatcct
      721 ggcggcaatg ctggtggtca tagccaccgt aaatggagtg gcatacttct ggcagtactt
      781 ccatgagccc acctttcctg gatacttcac tttgatcatg atctatctat acgagagcat
      841 ctggtggtac atggctgctg ccttatgcgt gaatctgggc aaggtcatgg acatgtatat
      901 ggagggcata agagacgctc gcaccatatc tttcctcttc ttcatcctgg ccaccggcct
      961 ggtcatctgg tcgaccagca gctttatcct ggccagcaat gccgatctgg agtttggcat
     1021 tggcatggta gaatcggtac agaagctggc catctcagct attggggcta ttctgattac
     1081 cgttatcggg atcaatgtat ccaaacgcat tgcgaccgat aatgccttcc ttccatcgaa
     1141 gtgatatttc aatgctccag atcctggcct ccggggagaa gtgccttggc gacttcacat
     1201 ttgactttta ctggcagcat caaggctcaa ggctggatat ggatcagaag atcggggagg
     1261 ctagcctcag gcagatcgtg caatcgctcc gcaaggggga gagggtgaat atctccgggg
     1321 atgttggcag ccgcctgggc tccagcctgg gcgtcgatct catccggctg gggggaaagg
     1381 gcgggccgat agaggggacg ggcagcatag tggtggaggg agacgtgggc cggcgcatgg
     1441 ggataagcat gctcagaggc gcgatctatg tctccggcag ggtcagtgag cccatgggga
     1501 atgtaatcga ggttgatagc gatctgactg gatatcgaat gttcgtctcc atcaccgagg
     1561 ctctggaaaa ggggtgggat gtgacggagc cgaatcatct ggaccagcag ggtctgttca
     1621 tcgaggacgg gattatcagg gataccctgg gggcaagaaa tcccgccgac aagaccatta
     1681 aactggaggg agacgcaggc atgagcacgg gcatactcat gcgctccggc cagatcatcg
     1741 tcgaaggatg cgctcaggag aacacagcag ttctattgcg aggaggaagg atcttggtta
     1801 gaggcagtac gaaggacttc acaggtgcag agatgagagg gggcgaggtc ttcatagaag
     1861 gagatgccgg cagcttcacc tgtgcccgga tgaagggtgg agtggtctat gcccggcagg
     1921 ctctgcccct gccaccagca aagaggcatc cactgagccc ttccgagagg actgtggtag
     1981 ccagggccct ggagctgggc ccaatgcagg ccctgatgta tagccggttc ggccttcagt
     2041 agatctcttc cagatcctct atatgggcga atgcgtcgtc tagctgcttt cttgcccttg
     2101 acctcttctc cttctcgtcc tgcatccctc ttgccgcctg agcatagatg gattcaagct
     2161 ccggtgcagc agccaatgtg aatacagaga gcactcttgg atggcccttc ttgaccacaa
     2221 ttcccttgaa tacgtgcccc gcgctggcgg tgatcttctc cacctctttg gttatctgat
     2281 ccacatccag atgctccttg gagccctgga tgatgaccat tgccctggcg gcgtcatccg
     2341 ccacgttcgc cggatagagg aggttgttag gctttagact gttctcgatg gcgtccttga
     2401 ccgagttgtt cttcccatct gcctgatagt atcccatggt gcctatgcgc aggccgccgt
     2461 tcatgaccgt cttgaagtca cccagatcgg taacggagag catctcactg tccagagatt
     2521 cgatcaggaa aagcaatctt ttggcgatgg tgctgttgat cttgtcatag gcgctggcaa
     2581 tgtcgccgct aaaccttttc atatactgat tgtctgccag gatgatgcca tcagcgtcca
     2641 gctccatcag ctccctcatg ctgaaagcag cattctggag gtagatcgag ccctcctccc
     2701 ggaagggaag aatagccaca gtgaccacgg tgatgttcgg gtactgcctc ttcagctcat
     2761 ttataaggag cggcgagaac gaggagccag tgcctcctga ggttgaggtc aggacaaagg
     2821 cgaggtcgaa gtcgcccctc ttctcgatct ctcccatgat gctgtcccgg ttgtccataa
     2881 aggcctgctt gcccacgttc ctgttggctc ccatgccgtg caggttcgga acatgcaatc
     2941 tgtctttggc ggtggtgtat ctcagctcct tgagatcgtt tatcgccgtg ttgatcgcca
     3001 ccgtctctac ccggctgggg aatttgggct tgagacagta cttggcgatc ttcgaggcac
     3061 ccccaccacc catcgcttcc ttgttcactg cgtccagtat tctatttccg cattggccta
     3121 caccaaggat aagtacgtta agcatgatat ccgcctcact ttactgtgga attttattaa
     3181 tatttatatt ctttgtgccg cctatacagc caggcggcga taattatgat tgggataagg
     3241 agtagtaaat attcatattt atactctcgt ttcagtacca ctacctctgc agatccttct
     3301 ctgatcgcag agccgtctcc tccatcgaat gtggtcttga ggtgaatggt atagttacca
     3361 gagtccttgc ccgaaagcat tactgagtaa ttgacctcgc cgcctggcgg tatctcaaag
     3421 aagctcctct cgaagcccga tgcctccagc cccactggcg gttttatgtc ccctttggcc
     3481 tcgattctgg tggcaggcgc ctttccaaca ttttctatgc ttatgttcgc cattccagtg
     3541 ccgccaagag ggagcggccc aggtacttcc atccttatcg ccaggtctgc cacgctcttt
     3601 ggattgatga cggtatcaaa aggctcagaa ctgttcaata ggacgttatc cttggagtcg
     3661 gtgtagctga cctgtatggc cgagacgcgt atggtgccct cttttatggc attcatcata
     3721 tatacagccg agtcggactg gccagggtcc agggtatcct tgatcttggg cggatagccg
     3781 gccacatagg agaactccgg caggggtggt gcatcatcca attttatgtt ctgtgctttg
     3841 cctgtgccct tgttcatgat ggtgactgtg accttgacct catcccccac actcatctcg
     3901 ctcttgtcaa agctcttctg gactgtgagg gctggcttgt caccggagga tacaggccgt
     3961 tccgaggctc ttctgctggg cgttcccagg ctctcctggt actcgattcg tactctggca
     4021 gactcatcat ccaggatatc tactaccgta agggttatac cccctttctc cgctcctccg
     4081 gcgaagttat tggcccggtt ctccaagaat gctctctgct cgtccagtcc gcctccggtt
     4141 ctggaaacct gcaggacagc tatgccatct ttgaccgact ggatctcaat gagttctgct
     4201 cgatagttgc tgatgtctat tctgtctccg atccccaggg tgaaatcgtt ataagtgacc
     4261 tcatcatcat catcgtcatc cgccccctgg accgctccaa tgaaggccgt tgacagcaaa
     4321 agaataagga cggccaggca gaccaggcgc agaatatcga ggtgctgctt cttgcccata
     4381 tctctatcct gcaagtcaat ggttctttta agcgatttct ctcttcgctc tccactcttc
     4441 atcgttctct atctccaggc tcaaatcaat ccatctagca tttttggtga gagcaccaag
     4501 cgatataaca tctaccccac ttcttccgta atccttgatg ttatccaggg tgatgccccc
     4561 tgacgcctcc agaatcactc tctctcgtag accctccttc ttcaagacat caactccctc
     4621 ggctatcttc gcggggtcca tgttatcgaa catgatgata tccgcaccct ctcttgccgc
     4681 tatgagcatg tcctcaaggc tctcgatctc cacctcgatc ttcttggtga agctggattt
     4741 atctttagct cggcgcaggc attctttcag gccgaggatc attatatgat tatcttttat
     4801 gaggatggca tcggatagat taaatctatg ggtgtcgccg cctccaagat agaccgctct
     4861 cttctcgaat aatcgaaatc ctggggtggt cttgcgcgtg gctgccactc gcaccccccc
     4921 ggccaggaga acgcactccc gggtgagggt ggatatgccg ctcatgcgag ccatgaagtt
     4981 taggaccagc ctttcggact gcaggatttt tctcgcgctg cccactacct ctaaaacagt
     5041 gctcatcgct ggcacaaact cgccatcatc gaatagcagc tcggatttca ggccaaaata
     5101 ctggaagatc tccaccgcct ccgccagccc ggagatgatg cagtcctcct tagcgatgat
     5161 gatcccgcaa gccgtcaatt ctggaatgag agtgctggaa tcatcccatt cgcctaggtc
     5221 ctcacctata aatcgctcca gctctgacct tagcataccg caaggatttt gatccttaat
     5281 tgatttataa cctgcgatgc ctctcaaggt cgggcttgtg ggatgcggtg ccattggggc
     5341 cgagatcgcc aaagcgatag attctggcgt ggttgatgca gagctgattg ctgtatgcga
     5401 tcataatcct caaactgccc tcgctctcat cgagagcttg cagcataaga cgaaaaaagc
     5461 cgatcttgcg gaattggttc gcctttcgga tgtggtggtg gaggcagcct cccagaaggc
     5521 ggtcccagca gtcgccaggg cagcattggc cgcgggcaaa aggctgatga taatgagcgt
     5581 tggggctctt gccgatatgg atctattcag agagatcaag agcctggcca aatctcactg
     5641 ctctcaggtc tacataccat ctggagccat atccggcctg gatgccctca agtccgctcg
     5701 cgccgggagg attgaaaggg ttaccctgac caccaccaag aatcccagcg gcctgagagg
     5761 ggcgccctat atcatggaga ataggatcga cctggatagc ctctccggtc ctacccagat
     5821 cttttccggc agcgcaatcg aggcggtaag ggccttcccc gcgaatgtga atgttgccgc
     5881 caccctttat cttgctgcgg gggagaaaga ggtgctggtg aaggtattcg ccgatccgaa
     5941 tataagagta aaccgccacg agatagaggt ggaaggcgat tttggcaggt tttttacccg
     6001 ggtggagaac gtcccctcgc cgcgaaatcc gaaaaccagc tatttggccg cactctccgc
     6061 catatccaca ttgcgctcta ttgtagagcc gatcaagatc gggacctgat cgaagacgat
     6121 ctaaaagagg ccgtgaacct cattcatctt ttccttcagg ccctgaaggg tcccagagag
     6181 ctctttattg atcctgttct cggtattcgt gccgaccctc atctctatat ccggattaga
     6241 gaggggctca tgcccggtga ggttcatgaa aagctctacc cagtaagggg gcagatactc
     6301 aggcagcggc tcgcctctca tgatatgaag gacactggtc caggcggtgg gcaccacctc
     6361 catattatag ccccctccgc ccagggccag cagccgggac cctgaatgct ggcgggccag
     6421 atccactatc cggcggacga ggccggtata gccgttaagt gtcacgttaa ggctggtgag
     6481 gggatcggaa tagtgggtgt ctatccccag ctgggctacc actgcctggg gccggaacca
     6541 ttcaaaaagg attggaacga tctgatcgaa ggcccggagg tattgctcgt ctccggagta
     6601 catgggcatg gggatgttca cgctgtatcc ttttccctca ccatctcccg tctcctctat
     6661 gaatcccgtg cctggaaaga gatacaatcc ggattcatgc atggagatgg tcaaaacact
     6721 gggatcagtg tagaatatct gctgcacccc atcgccgtga tggccatcga tgtcgatata
     6781 cagcacccga tcaaatctat ctcttaaaac ccggatggcc agggctggat cgtcaaagac
     6841 acaaaacccg gaggcgactg tgggcagggc atgatgcagt cctccgccga tgttaaaagc
     6901 gctgcagtcc tcagctatta tgcggcgggc ggcatcgata gaggcccctg ccagcattct
     6961 ggatgactca catatgcccg cgaagaccgg ggtgtcgtcg ctccccaggc caaaggccag
     7021 atccggctcc tccagcttca ccgccgccag gtactcgggg gtgtgcactc ggagaatatc
     7081 ctcttcgcta gccgggcggg gctcaagcct gcataccttc tcgtctatta gaccagactc
     7141 ctccatgagg ctaaaggtga gcatcaggcg agcgggattg aagggatgct ccggaccgaa
     7201 gttataccct tgaaatttct cgctgtaata aaggtagatc atttatagcc tttctctatc
     7261 aggccgtcgc tccagtagtt atcacagttc ttgcagtaat agatcgtctc gatcacaatc
     7321 ttcttccatt catctgcgtc cagtgcccgc cggaggtctt tatgctcgca ctcgttgccg
     7381 catttggggc agaatggtat gaagtgttcc aggactgcgt ggtcttcatc agccatctga
     7441 tattcctctt tgaggcagag tgtcgcggga taaggcaaaa agctgtcggt gccagaacta
     7501 taccgtgccg ggttagaagg gctggatgcc tgcggcaaga cagccggcga gaaatccgag
     7561 gatggatccc gagttcagga agggaaggcc agcctgaggt cggtccctcg atgtagtcat
     7621 cagaaggata aagccggcat aggttccgag catggctcca agagcaggaa ggttcaggcc
     7681 gaggatatca cctggtggat atgaccagct cgcagatatc accaggatgg atggtatgat
     7741 ggcatcgccc atgcccagga aataggctcc acgatccccc ttcttcttgt cgtttcccat
     7801 tgagatcgag actgcatctt ttcgcctaaa gctgtagtcc ctgctcttgg ggactacaaa
     7861 aagcagtggt gccttcatct ttattgcgcc ctctgccaaa gcgaccatgt ggcgggtttt
     7921 atagacggag atggcatcgt agaccgccag gatcaccata aggatgataa ccggcatcac
     7981 ggtcatgctc actcccagga gggaggtaac tcctgcgcac accaggattc caaatgcatc
     8041 cagaatatac cattccgggt agtagtgcaa gagcagcatc accgccaaag ttggcaggag
     8101 ggcaagccac atgggcatga tggcggagat cacatagaag atggtgaggg caatggagat
     8161 gagtatgaat cctctcacca gccaatctcc cttcagtttt atggccaaaa ggagaatggc
     8221 ggtgaatatg aggatgagca gcatatagat tatgggattg gagatcgatg ccggatcctc
     8281 gaatacccga ttgtctgtgg cggatatggc cggagttatg gccagggcta tcagctgcac
     8341 cgctaccagg aacgagagca tgaccagcac atggaatttc gagcttatgg acaagatatc
     8401 atttccatta tatcgtcatc ggtctataga tctgccctct tctcctctag caattctatg
     8461 tatgagcggc ggatggtgtc ctccaattcc agccgcttca acacggagag gacccgatcc
     8521 ttttcgttct cccagttcgc ttctgcctga gcctccacct ccaaaaaggt gccaagccct
     8581 tccacctcgt ccagagcgat gaccattccc tgataggagt acttcgtcct ctttttgcga
     8641 acctttgccg ataaaacaaa acccaaaagg gataggacat ccctcatctg ggtgggatcg
     8701 tcgatcctta tcgtcagctc ccggcgcgac ttggtctgct ggtcgagctt gggcccctta
     8761 taggtgagcc tggccccatc ctccttaatg cgaatgcgca gagcctcgtc gctctccttg
     8821 aagtccctta atggcgaatt gaaataaagg tccagatgat tctccacccc gatgggggtg
     8881 gctcccaggg cggctattct ctcttttgtg tcctccctgg cccgggcttt gacctccacc
     8941 tcgatcatct tcggaccagc accgtggcct ctttcgtctt cagtatatcc accgcctcgc
     9001 cagcagagag agtctggatc tccacctccg ccgcctcggg cggaagctcg aggatctcgc
     9061 catccagatc gactgcaatc gagcctgcct ccttctggcg agcgatatca gccggatcca
     9121 tgcccatcag ggccataatg atccgctggc tcctatccct gaatcgaggg ccgatgacct
     9181 tcatttgcgg tttgaccgct atgggcttca tctccagcga gggctttcct gttctgctct
     9241 ccaccgggga gttggccaca ccggccaggt ctgctgtctc caggtcgagg tcggagtata
     9301 ccacgattcc cggaagaggg gagttgaggg ccactccctt ctccgcctta tagcggcgca
     9361 gagttgaagc tacctctttt atcgccaggc cagccctatc catcggctgc ccccgggcca
     9421 cgggccagct ttgcacatga acgctctggc cagtcagggt gtggtagatc tcctcggaga
     9481 ggaagggggt gaatggagcc atgagtcggg caagagtctc cagggcggtg aaaagggtgt
     9541 cctgcgctgc ccttttctcc ggcccgtcct ggccgtacag ccgggccttg accagctcga
     9601 tgtagttgtc ggccagaaca tcccaggcga aagcccggat ggccttcaag gcctcatcga
     9661 actggaatct gtccatagcc tccgtggtct tagtgatcag ctgatccagc tctgcagaga
     9721 gccagcggtc gacttgccct ttagcggcat cagccggggc gatcaggggc agagagaacc
     9781 ggaatatgga ccagagcttc tgctggaatc tgctggcggc agttatctcc ttccactgga
     9841 atggtatgtc atttcccggg ctgccgccga tagccgccca ctggcggagg gcatcagctc
     9901 cgttgctctc gaatacctcc tccgggcgga tgaagttgtt cagggattta gacatcttat
     9961 ggccgtcctc gcccaacgcc atgccattga tcagaatggc atcccagggc ctgatccctt
    10021 ccagggctcc ggttctgaga atggtgtaaa aggcccaggt gcgaatgatg tcgtgcccct
    10081 gcggccggag ctgggtgggc atacgcagat cggtcctgtc cggccagccg gcgacggcta
    10141 gggcggatat ggagctgtcc atccaggtgt ccagaacatc ctgctccgga atgaattccg
    10201 agctgccgca tttgcatttc tttttgggct gggtcaggtt ggggtccagg ggaagccagc
    10261 tctcctctgc caccagtgcc tcatgacaat gacggcagta ccaggcgggt atgggggtgg
    10321 caaagatcct ctgcctggat atgcaccagt cccattccat ggagtcggcc cagttctcca
    10381 ggcggacgcg catgtggggg gggatccatt ctatctcctc tgccaccttt tttatctccg
    10441 gggcatcgat tcggacaaac cactgctgct cggacaggat ctcaatgggg gtcttgcagc
    10501 gccagcagag ccctacgttc tgctccagag gctcttgctt gaagattatc ccctgctcgg
    10561 tcagatcctg ggtgatcttc tctttcgtct cctccacggt caggccggag taagggccag
    10621 caataggcat catcctgccg ccgcggtcta tggcctggcg gaggggaaga tgatgctcca
    10681 tccaccatct gacatcctgc ttatccccga aggtgcagat catgacgaac cccgagccga
    10741 agctcgagtc cactgccggg tcggcaagaa cgggaacgga atagttgaat agcggcactt
    10801 tgaccgtctt gccgatgtat ttctgatagc gctggtcctc aggatgaacc gccaccgcca
    10861 cacatgcggg caggagctca ggccgggagg tggcgatctc tatctcgccg tcctcagcac
    10921 aaaagtgcat ataattcagt gtcgaggtgc gggagtcgta ctccacctcg gccagggcta
    10981 tggccgttcc gcagcgggga caccagttta ccgggtggtc ctctcggtag atctggcctt
    11041 tctggtacat gcgcacaaat gaagtctggg tcttgacata atactccggc ttcatggtgg
    11101 catattcatt ggaccaatct atggacaggc ccagccggcc catggactta tggaaacgat
    11161 ctattgcctc ctcagtaagt ttttcgcaga gccgtctgaa ctcctccctg ggaacctggt
    11221 tcttggtgat atggtttagc tgctccacct tcacctcggt gggcaggccg tggcagtccc
    11281 agccctgggg gaacatcaca ttgtagcccc gcatgcgctt gtaccgggcg gcgaaatcaa
    11341 tgtagcacca gtttagagcg ttgccgatat ggaagtttcc cgtgggatag ggcgggggtg
    11401 tgtcgattat atattggggc ttcttcgact cccagtcaaa atagtaaatg gaactatccc
    11461 agctccgctc ccatttctct tctacctgct tgaaattgta ctctttggac agctcgctca
    11521 ttaaatggct ccctgctatt ggctgctagg ggaaggaaca caatgttaat aggattatcg
    11581 gtagtatcct ccatgaatgg aacttgtgat ctatcatgcc aatcagtgcg acccccggaa
    11641 gtgttcagga aagaagcttg cccgcttcga tcttgtccgg ctgacccacc atataagcca
    11701 gctccggccg tacattgtcc tcagcccgtt ctcggaaaag gccctatccc cggaggataa
    11761 aggggcccgc gggctggcag cccttgactg cagctgggcg catgctgagg aggtcttcgc
    11821 ccggttcaag ctgcaggaga gggctctgcc ttttctagtg gctgccaacc cggttaactg
    11881 gggaaagccc ttcaagctct ccaccgtcga agccctggct gcaggattgg taatactggg
    11941 agagaaaagc caggcagagc tgatcctctc caagttcaat tgggggcatg tcttcctgga
    12001 gctgaaccgc gagcccctca gtgagtacgc cgctgccagg gattcagctg aggtggtcag
    12061 gattcagaat gaatatatgt cggaatagat ttaagcgcca aaggcccata atattatggt
    12121 gttgaggatg accgcaggat tgattccggc agaagaattg cgaaagctgc agttcaggat
    12181 gaataaattg atggatgagc tgggcctgga tgcgctcgga tccaagtata tcgatgagct
    12241 cgggcggatg caaaagcgca tgggcgacct tatggaggag gtagagggcc cgaacctgaa
    12301 gaaaggggtg ataaggcccc tggccgatgt tcatgagact gaagaggcgt tggttgtgac
    12361 catggatatg cccggtgtgg aaaagcagga tatcaacatc tcagtggtgg aggatgagct
    12421 gcagatctcg gcccagagaa agagtgagac cgaggcaaat gagcaggact accaccgccg
    12481 ggagaggacc ttcacccggt tcgagcgaag ggtgctcctg ccggaaagca taaagacaga
    12541 ggaggcgcgg gcgactcttg cgaacggcgt tctgcagata
//
LOCUS       DAOSGP010000009        12018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_4487_length_12018_cov_12.589401, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000009 DAOSGP010000000
VERSION     DAOSGP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12018
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4487_length_12018_cov_12.589401"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1310
                     /locus_tag="PLV49_00625"
     CDS             132..1310
                     /locus_tag="PLV49_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97374.1"
                     /translation="MERLMPLPPEETESKERKRIGLIGSGFISKGLVVLIEPRHDLQI
                     HRILTRSRIEERQDFPRPDLLTNSYEELLQDADLILEASGDIFSSTAVLEMALERSIP
                     VITMNAELQITTGSCLARKGFITEAEGDQPGCLAALREEVLQMGLSPLVYGNLKNFLN
                     RNPTLEEMQFWAAKSGNSVENITSFTDGTKVQIEQALVANGLGAAIIKPGLCGLASDS
                     VEAGGIELAEKAKAMGTTVSDYLLSAKIPPGVFVTAECDPCHKVYLRYYKMGEGPYYT
                     FVRNYHLCHFEAMKTIDRVLGGGDVLINNSLQPRISVAAIAKRDLLAGHRIKKAIGSF
                     ELRGEAVNIRDQPNHIPIGLLQDARLKEDIRAGETVTFDRVSIPESLALQFWQKGAGI
                     "
     gene            complement(1323..2093)
                     /locus_tag="PLV49_00630"
     CDS             complement(1323..2093)
                     /locus_tag="PLV49_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOG97375.1"
                     /translation="MIDAAQAIFLGILQGITEWLPISSEGQNMLVMIGWLNISPQEAF
                     SCSIFLHLGTMFAVLIRFKDEFINALKNTGSQMTRILIISTICTAITGIPLYLLFREG
                     FQGGMPATLLIGALLILTGLLLRLKGSGARSIQDMNALDMVILGLAQGFSILPGVSRS
                     GTTLTVLLMRNLKQDDSLAISFMISVPAVMGALILDHSLGQMSLASAFLALLASFGAG
                     YLTMDLLIAYAKKVNFSGFCITMGLLTLFLAYIFKAAG"
     gene            2342..3328
                     /locus_tag="PLV49_00635"
     CDS             2342..3328
                     /locus_tag="PLV49_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97376.1"
                     /translation="MELVIIIGIVISFLATWLTTRKWIRKAPEIGILGFDMNKPGLPK
                     VAEMGGIGVVFGFGTAMLLYIGLITFYLEKSNYVSILAVLCTVLISCIIGMMDDLLGW
                     KKGLRQWQKPIFTFFAALPVMVINAGQSTMNLPLIGSIDFGILYPLLIIPIGIVGASN
                     VYNMVAGYNGLEAGMGAIILSTMGYFAFISHSQNAMILAFCMVSALIAFLYFNWFPAK
                     VFPGDTLTYSLGALVACVAILGDMERMAIILFLPYALDFAMQAAGGFGKEAFARVNPD
                     GSLEKPYARLFHLTHLAIAVLKRLKAKVYERDVVLLLCGTEAAIAGLCIYIY"
     gene            3355..4179
                     /locus_tag="PLV49_00640"
     CDS             3355..4179
                     /locus_tag="PLV49_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOG97377.1"
                     /translation="MVDEIDLRDIVRVLSKRRLMIIGVFVFSILVAGVISFAVPPVYK
                     VSSIIAIGYFDDPAYTSQASMKNVMISDEFLLEVFEAISPNGSSGDFRDFKENVEVEP
                     VKDSDRLIEISIETEKRKEGLNAVEIMIERYSELSENSYNKQKKILEDQMANTEQRLE
                     VIDMEINQTRETLKEIEERSGSSAVQAEMRFSRTLDILNGKESQRSALLDRRMELQKQ
                     LLLIRNLEVIQPPREPISPVWPRKALIIAIAGMLGLMGGVLAAFLQEGLGGRPAEQ"
     gene            4194..5105
                     /gene="rfbD"
                     /locus_tag="PLV49_00645"
     CDS             4194..5105
                     /gene="rfbD"
                     /locus_tag="PLV49_00645"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOG97378.1"
                     /translation="MIAARPGGDALSQAQEHKGNMRVLVLGSNGQLGSEICRVMQEDD
                     IIPARRCDADITQLSQVVQFACHLRPDVIINAAGYTDVDRCESGRERAFLDNAIGARN
                     AAIASYKAGAELIHVSTDYVFDGNKDGPYIECDLPQPLNIYGWSKLLGERMAMEQNPN
                     TFILRTAWLYGSVGRNFPKSILALAQEKDEISVVNDQWGTPSYAGDVARQIKLLMDTK
                     SYGLYHCTSQGGCSWYDYAREIIRLAGLQVRVVPVASSDYPRPARRPANSVLDNLMLR
                     AQGLDVMPDWKDSLAQHIGEIMESLKK"
     gene            5353..6924
                     /locus_tag="PLV49_00650"
     CDS             5353..6924
                     /locus_tag="PLV49_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOG97379.1"
                     /translation="MGNPELTENKNQVQGIQISGSLIARNAVLNLIGQALPLLVAVIA
                     MPFVVRGLGTERFGLLSLAWVVMGYFTIFDLGLGRATTKFVAEALGKGEGSRVSQIIW
                     TAVTVQAIMGAIGALALYGATDYLVQRVLNIPPALIYEAEVTFHLLAFAIPVVMLTSS
                     FSGVLEATQRFSLVNAIRIPSSTLTYLLPLVGLYLGMGLHGIVALILLARLAALIAFV
                     AINLHIRPELRFYSASFKLLSRLASFGGWITVTGIVGPILVYLDRFLIGSLLTIAAVT
                     YYTAPYEAVTRLFIISGSLTMTLFPAFSALEGKKNSHRLGALFARSIKYILITTGAFA
                     VMIWLFAEEILQIWLGADFVSESTASLQILALGVLINSLSYAPFALLQGLGRPDLPAK
                     FHMIELPAYIIVVWILISNFGISGAAGAWTLRVALDALLLFWAALKVSNLSLNVMENN
                     GAILAGASLVILAGVGYVMKTVTGDLSLYAQSFLVLGLLIPFAWMVWSRVFDGSDRRV
                     VLNIIKAQEIAGSTQ"
     gene            6921..7985
                     /locus_tag="PLV49_00655"
     CDS             6921..7985
                     /locus_tag="PLV49_00655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG97380.1"
                     /translation="MIENEGIRVEETRSCLLCENEGNLLYGGLRDFLFGAPGIWSLMR
                     CPNCGLVWLNPRPLPDDIGKLYSQYYTHQIPEHPQKNLAGIRKRAKASILQSCFGYHM
                     DGSNRILGSVLGRIGLLEEIIGAGVRYLKAGDKGRLLDVGCGNGLFLDRMRQYGWDVM
                     GVETDGCAVSVAREKFGLEVFHGSLEEAMFPEGCFDAITVNHVIEHISDPIRLLKECC
                     RLLRPYGKLAIITPNMDSLGAHLFCECWRGLEIPRHQFLFTQKTLRACIEAAGLDVQD
                     ARTTANLACFIWAASCSIKKKEESLERPLDVPSNMVRLEALAFQILEYGLCWLKEYGE
                     EIVVLASKKNESGIDKAMRK"
     gene            8064..9206
                     /locus_tag="PLV49_00660"
     CDS             8064..9206
                     /locus_tag="PLV49_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HOG97381.1"
                     /translation="MICIVLGLAAVRRCSLYSPLVINASIWLIVFLAGLFFENRFYPI
                     QEKAFIAWLIWFMVTSLMFFLLYPYRAKYNWIGNEIRKIPIDYSLLLLVLIVWLGYRI
                     WVVGSSGPEHFFLNLRLSSMNLEGIPSLGLILRFYPLVFTLFLFEEVYACRENRHLRI
                     LLWCFMILYAMAMMGKSSILTPFFSWAIIQGIKGRLKAKMIVILVLVVFASMISLHVI
                     RSSTSYVFSTVDILAIYIYSPLVALGYMNIDSSLPVGAYVFRFFYVIGNILDIASHPA
                     STITPYVAVPELTNVYTVMQPFYHDFGMLGVLLEAVIYGLFFSILYFLSVKRGGFWLI
                     LFSGYSIVLVMQFFADLLISRLSLNLQLLLYALIIFWTSRKVQYAR"
     gene            9285..10193
                     /locus_tag="PLV49_00665"
     CDS             9285..10193
                     /locus_tag="PLV49_00665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG97382.1"
                     /translation="MKQVEVLLATYNGSQYVVPLMNSLLNQDYKNIKIIVRDDGSNDN
                     TLEVLNRYTSYENVSIIEGEHEGVPESFFSLLQLSSSDSHYFAFSDQDDVWQRDKISR
                     AIGFLGRLPQELPSMYCSRVLIVDEMLNPIDYSPIPKRAPSFENAIVENIATGCTIVI
                     NKAARDLLLNNSPKNALMHDWWTYLVVSAFGEVFYDPEPRILYRQHSSNVVGCKIGFI
                     NRWSSRVERFLKYSSLRRPTRQAMEFDEIYGASLPKDKKLVLDRFIDGRNTHMGRVLY
                     ALNGEIYRQSIIDSIILRVLILLNRI"
     gene            10326..11480
                     /locus_tag="PLV49_00670"
     CDS             10326..11480
                     /locus_tag="PLV49_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG97383.1"
                     /translation="MNSRIIICVIGNQAFSLLNFRGSLIAEMVAGGAKVLALAPDYDE
                     HTRAEVRAFGADPVDFSLSRTGMNPIRDVKDMLRLAFLLRKLKPDISLGYAIKPVIFG
                     TLAAWLARVPRRFAMIEGLGYIFTPAEGADQFKRRVLKGIAIMLYAMALKPANLVFFL
                     NKDDIDEFSKKRIISPKKAFLLGGIGVDLNEWVSTSQVMKPVTFLLAARLLREKGVME
                     YAKAARLIKQKHPNTRFVLLGDIETNPGSLSCDLIKAWDAEGILEWLGHVPDVRFWME
                     RASVFVLPSYYREGVPRSIQEAMAMARPIITTDAPGCRDTVIDGLNGFLVPVRDVCAL
                     ADSMERFILQPELIDEMGKASRSLAEERFDVHKANKIILHEMGLDELNII"
     gene            11642..>12018
                     /locus_tag="PLV49_00675"
     CDS             11642..>12018
                     /locus_tag="PLV49_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97384.1"
                     /translation="MNEANNRYVWTKLALLLIILPIFIGLVCVIKFGVNVPYWDEWDI
                     QTKTLKNYYEDSLSLNDLFSQHNESRPFFPRIVLLVIDLITNYNIVAEMIILFLIYCL
                     SFLIIFLMFRKDNLGNKSYLWLF"
BASE COUNT         3066 a         2608 c         3086 g         3258 t
ORIGIN      
        1 ttataggaat aaatccgcca gagcggaaag aattggttga agatgagcaa gaatgtattc
       61 tatattttct atcacctcat gatgcctgct gcgaagctat ttaatcgagc agaaaagagg
      121 cagatcctga tatggagaga ttgatgcccc tgcctccgga agagaccgaa agcaaagagc
      181 gaaaaaggat cggcttgatc ggctcaggat tcatatccaa gggcctggtg gtgctcatcg
      241 agccgaggca tgacctgcag attcacagga tcctgaccag gtcgcgcatt gaggagcgcc
      301 aggactttcc ccggcccgat cttctcacca actcttatga agagctgcta caggacgcag
      361 acctgatact ggaggccagc ggagacatct tcagctccac cgccgttctg gagatggctc
      421 tggagaggtc cattcctgtg atcaccatga atgccgagct gcagatcacc accggctcct
      481 gtctggccag gaagggcttt ataaccgagg cggagggaga ccagccaggc tgcctcgccg
      541 cattgcggga agaggtgctg cagatgggcc tctcacccct ggtttacggc aacctgaaga
      601 attttctcaa ccgtaatccc acattggagg agatgcagtt ctgggctgca aagtcgggaa
      661 acagcgttga gaacatcacc tccttcaccg atgggaccaa ggtccagatc gagcaggcac
      721 tggtggccaa cggcctggga gcagctatca taaagccggg gctgtgcggt ctggccagtg
      781 acagcgtgga ggcggggggc attgagctgg cggagaaggc caaggccatg ggcacgaccg
      841 tctccgacta tctcctctcg gccaagattc ctcccggcgt ctttgtcact gccgagtgtg
      901 atccctgcca taaggtgtac ctgagatact acaagatggg agagggcccc tactacacct
      961 tcgtgcgcaa ctatcacctc tgccacttcg aggccatgaa gaccatcgac agggtgcttg
     1021 gaggcggaga tgtgctcatc aacaactccc tgcagccacg gatcagcgtg gctgctatag
     1081 ccaagaggga ccttttagcc ggacatcgga taaagaaggc cataggcagc ttcgagctgc
     1141 ggggagaggc ggtgaacatc agagatcagc caaatcacat tcccatcggc ctgctccagg
     1201 acgccaggct gaaggaggat atccgggccg gggagacggt cacctttgac cgggtcagca
     1261 ttcccgagag cctggccctg caattctggc agaagggggc ggggatttag ttttccccac
     1321 cctcaccctg ctgctttaaa gatgtatgcc agaaaaagag ttaatagccc catggtgatg
     1381 cagaatccgg agaagttgac cttttttgca tatgctatca gaaggtccat ggtgagatac
     1441 ccggccccaa aggaggccag cagagccaga aaagcagatg caagggacat ctgccccagg
     1501 gaatgatcca gaattagtgc ccccatcacc gccggcacgc tgatcataaa ggatatggcc
     1561 agagagtcat cctgtttaag gtttctcatc aagaggacag ttaatgtggt cccggatctt
     1621 gagacgcctg gcagaatgga gaatccctgg gcaagaccca aaatgaccat atccaaggca
     1681 ttcatatcct ggatactgcg ggcacctgat cctttaagcc tgagaagcag tccggtgagg
     1741 atcagaaggg caccaataag caatgtggcc ggcatgcctc cttgaaatcc ttcccgaaag
     1801 agaagataaa ggggaatgcc ggtgatagcg gtgcagattg tggagatgat caggattctg
     1861 gtcatctgtg agccggtgtt cttcagagca ttgatgaatt catccttgaa ccggatcaat
     1921 actgcaaaca tggtgcccag atgaagaaag atggagcagg aaaaggcctc ctggggagag
     1981 atgttcagcc agccgatcat aacgagcatg ttctggcctt cgctgcttat gggcagccat
     2041 tctgtgattc cctgcaaaat gccgaggaag atggcctggg cggcatcgat catcaggcta
     2101 agagatttga agcatatatt tgaaagtatg caggatagcc cgatttcatt gcagggcgaa
     2161 ttctcagaat gatttaggat tataatatat aaataattaa taagataata agatgtcatc
     2221 atcaagtgaa tcccaatcaa taaaccgata gagacagaag atgatgatat gagctttgct
     2281 ctgtttttac ttaatttttc gggaaggctt atatagtttt caatggatac cttttgacga
     2341 tatggagctt gttataataa ttggcatagt gatcagcttt cttgccacct ggctcacgac
     2401 caggaagtgg atacgcaagg ctccggagat cggcattctg ggttttgaca tgaacaagcc
     2461 gggcctgcct aaagtcgctg agatgggcgg gatcggcgtt gtatttggat ttgggacagc
     2521 catgctctta tacataggat tgatcacttt ttatcttgaa aaatcgaatt acgtctcgat
     2581 tctggccgtc ctctgcactg ttctcatatc ctgcatcata gggatgatgg atgatctgct
     2641 gggatggaag aaggggctgc gacagtggca gaagccgatc tttaccttct ttgccgccct
     2701 gccggtaatg gtgatcaatg ccggccaatc caccatgaac cttcctctta tagggtccat
     2761 cgattttggg atcctctatc cgcttttaat aatccctatc ggcattgtcg gagcctcgaa
     2821 tgtctacaat atggttgcag gctacaacgg cctggaggcg ggcatgggag ctataatact
     2881 gtccacaatg ggctatttcg catttatttc ccacagccaa aatgccatga tcctggcctt
     2941 ctgcatggtc agcgctctga ttgcatttct atatttcaac tggtttccag caaaggtctt
     3001 tcctggagac accctgacct actccctggg tgctctggtg gcatgcgttg ctatattagg
     3061 ggatatggag aggatggcga tcattctctt cctgccctac gccctggact tcgcaatgca
     3121 ggcggcggga ggattcggca aggaagcatt cgccagggta aatccggacg gaagcctgga
     3181 aaagccttat gcccggctat ttcacctcac ccacctggct attgcggtgc tgaaaagact
     3241 taaggctaag gtttatgaaa gagatgtggt gctgctctta tgcgggacag aagcagccat
     3301 agccggatta tgtatttata tatattgaaa ttgagactag cctgaggcgg tataatggta
     3361 gatgagatcg atctgcgcga tatagttcgt gtcctgagca agaggcgcct tatgatcatc
     3421 ggcgtctttg tgttttccat actggtggct ggggtgataa gcttcgctgt gcctccggtc
     3481 tacaaggtct cctccatcat cgccatagga tattttgatg atcccgccta caccagccag
     3541 gcctcgatga agaatgtcat gatcagcgat gaattcctgc tggaggtctt tgaggccatc
     3601 agccccaatg ggagctctgg ggatttcagg gattttaagg agaacgtcga ggtagagccg
     3661 gttaaagaca gcgacaggct tattgagatc tcaattgaga cggaaaagag gaaagagggg
     3721 ctgaatgcag ttgaaattat gatcgagcgc tattctgagc tcagcgagaa tagctacaat
     3781 aagcagaaaa agatcctgga agatcaaatg gcaaatacag agcagcgact tgaggtcata
     3841 gatatggaga taaatcaaac ccgagagacg ctgaaggaga ttgaagagag atccggttca
     3901 tcggcagtcc aggccgagat gcgcttctcc cgcactctgg atatcttgaa tggcaaggag
     3961 agccagcgct ctgccctcct ggaccgcaga atggagctgc aaaagcaatt gctgctgata
     4021 agaaatctgg aagtaatcca gccgcccaga gagcccatca gtccggtatg gccgcgcaaa
     4081 gccctgatca tagcaattgc cggcatgctg gggctgatgg gcggggtgct ggccgcattt
     4141 ctgcaggagg ggcttggcgg gcggccagcg gagcaatgag atgatgagct ggattgattg
     4201 ccgcccggcc cggcggggat gccctctccc aggcacagga gcataagggc aatatgcggg
     4261 tattggttct gggctctaac ggccagcttg gatctgagat atgcagggtc atgcaagaag
     4321 acgatattat acctgcaagg cgctgcgatg ctgacatcac ccaattatcc caagtggtcc
     4381 aattcgcctg ccatctccgc cccgatgtca taatcaatgc agcggggtat accgatgttg
     4441 accgctgcga gagcggcaga gagagggcct ttctggacaa tgccataggc gccagaaatg
     4501 cggccatagc gtcctataag gccggagcgg agttgattca tgtcagcacc gattatgtat
     4561 ttgatggaaa caaagatggc ccgtacatag aatgcgatct gcctcaaccc ctgaacatct
     4621 acggctggtc gaaactcctg ggggagagga tggctatgga gcagaaccct aataccttca
     4681 tcctacgtac cgcctggctc tatggttcag tgggccgaaa tttccccaag agcattctgg
     4741 ccctggccca agagaaggat gagatcagtg tggtcaatga tcaatggggc actcccagct
     4801 atgccggaga tgtggccagg cagatcaagc tcctcatgga tacaaagagc tacggcctgt
     4861 accactgcac ctcgcaggga ggatgcagct ggtatgatta cgccagagag atcattcgtc
     4921 tggccgggct gcaggtcagg gtcgtgcctg tggccagctc tgactatccc cgcccggctc
     4981 ggcggcctgc caacagcgtc ctggataatc tcatgctccg ggcgcagggc ctggatgtca
     5041 tgccggattg gaaggattcc ctggcccagc acatcggtga gatcatggag tctctcaaga
     5101 aatgatccag caggacgcat tgaccagctg cctctggagg atgatttgcg ccattctccg
     5161 gtctgtgcca gcatcgtggg ggagccgaaa tgcaggccct ctggggggaa gttcgggatt
     5221 agcctgtccc agtggcaaag ctctccccca gactcagaaa gccaggatct gatgtcaggg
     5281 caatctcaga cgagggcatc tggttaagca gaaggatcag cagcaaaaag gtgagaggag
     5341 acggctggcg atatgggcaa cccggagctg acggagaata aaaatcaagt ccaggggatc
     5401 cagatcagcg gcagcctgat tgcccgcaat gctgtattaa acctgatcgg ccaggcgctg
     5461 ccgctgttgg tggcagtgat tgccatgcct ttcgtggtgc gggggctggg aacggagcgg
     5521 ttcgggcttc tctcgctggc atgggtggtc atgggctact tcaccatatt cgatctgggc
     5581 ctggggaggg ccaccaccaa gtttgtggct gaggcactgg gaaaggggga gggaagccgg
     5641 gtctcgcaga tcatctggac ggcggttacg gttcaggcga ttatgggcgc aataggcgca
     5701 ctagctctat acggagctac tgattattta gttcaaagag tgctgaacat cccgccggcg
     5761 ctaatttacg aagcggaagt tactttccac cttctggcat ttgctatccc tgtggtaatg
     5821 cttaccagct catttagtgg ggttctggag gcaacacagc gattcagcct ggtaaacgca
     5881 ataagaatcc cttccagcac tctaacctat cttctcccgc tggtgggact ttatctggga
     5941 atgggtttgc acggaatcgt tgctttgatt ctgctggcta ggctcgcagc cttaattgct
     6001 tttgtggcga tcaacctgca cattcgtcct gagttgagat tttattctgc ttcatttaag
     6061 ttattgtctc gcctggcttc ctttggagga tggattactg taaccggtat tgtcggtccc
     6121 attctagttt atctggaccg attcttgatt ggctcgcttc ttacgatcgc tgctgtaaca
     6181 tactatacgg ctccatatga ggcagtgacg cgcctcttca taatctctgg tagccttacc
     6241 atgaccttat tcccggcgtt cagtgctcta gaaggcaaaa aaaatagtca taggctaggc
     6301 gcgctttttg cccgctccat taagtatatt ctgataacca caggtgcctt tgcagtcatg
     6361 atatggcttt ttgcggaaga gattctgcag atatggcttg gagccgactt tgtctcagag
     6421 agcacggcgt cgcttcaaat actcgcctta ggtgtcctga tcaattcgct ttcttatgca
     6481 ccctttgcac tccttcaagg cttaggacgg ccagatcttc cggctaagtt tcatatgatt
     6541 gaacttcctg cttatatcat cgtggtctgg atattgataa gcaattttgg aatcagtgga
     6601 gctgctgggg catggacgct tcgtgtggcg ttagacgcgc tcttgttgtt ttgggccgca
     6661 ttgaaagtct ccaatttatc gcttaacgta atggagaata atggagctat cttagcaggt
     6721 gcctctctcg ttattctggc tggagtcggc tacgtaatga agacggttac aggagatctt
     6781 tccctttatg cacagtcttt tcttgttcta ggacttctga tcccatttgc ctggatggtt
     6841 tggagtcgtg tttttgacgg ctcggaccgt agagtagttc tcaatataat aaaggctcaa
     6901 gagatagctg ggagcacgca atgattgaga atgaaggcat tcgggtagag gagactcgaa
     6961 gttgcctgct gtgcgaaaat gaagggaacc tgttgtacgg aggtctgcga gatttcctct
     7021 tcggagcgcc aggcatctgg tcgctcatgc gatgtcctaa ttgcgggtta gtatggctta
     7081 atcctcgccc gcttccagat gacattggaa aactctattc tcaatactac actcaccaga
     7141 ttccagagca tccccaaaaa aatctagcag gcattcgtaa gagagcaaag gcaagcatct
     7201 tgcagtcctg ctttggctat catatggatg gctcgaatcg gattcttgga tcagttttag
     7261 ggagaatcgg cctgcttgaa gaaatcattg gagctggcgt tcggtattta aaagccggtg
     7321 ataaagggcg tcttcttgat gttggatgcg gaaatggatt gttccttgat cgaatgaggc
     7381 aatatggatg ggatgtaatg ggcgttgaga ccgatggatg cgccgtatcg gttgcacgag
     7441 agaagtttgg gctggaggta ttccatggat ctttagaaga ggcaatgttt ccagaggggt
     7501 gctttgatgc aattactgtg aaccacgtca tcgagcatat ttcagatcca attcgacttt
     7561 tgaaggaatg ctgtcgtttg ttacggccat atgggaagct agcgatcata acacctaaca
     7621 tggatagctt gggagcgcat ttattttgcg aatgctggcg aggattagag ataccacgcc
     7681 atcagttcct cttcacccaa aagacactgc gggcttgcat agaagcagca ggattggacg
     7741 ttcaagatgc aaggacgacg gctaatcttg cttgttttat ctgggctgcc agctgctcca
     7801 tcaagaagaa ggaagagagc ctcgaaagac ctcttgatgt ccctagcaac atggtcagat
     7861 tggaagctct agcatttcaa attttagagt acggtttgtg ctggcttaaa gagtacgggg
     7921 aagagattgt ggtattagcg agcaagaaga atgaatctgg aatagataaa gctatgcgga
     7981 agtgataatg tcttggcatt cttgaaatac ttcggatccg actaggtggt tgaatgattt
     8041 tttaccacct atccgcccta gtattgatct gcatagtgct tggcctagct gcagtgcgcc
     8101 gttgttccct ttattctccg ttggtgataa atgcatctat ctggttaatc gtgtttcttg
     8161 ctggtctttt ctttgaaaac cgcttctatc caattcagga aaaagcattc attgcctggc
     8221 ttatttggtt catggttact agcctaatgt tttttttgct ttacccatat cgtgcaaaat
     8281 ataattggat aggaaatgaa atccgcaaga ttcctataga ttactcattg ctgctattgg
     8341 tgctgattgt ctggttgggt tacaggatat gggtagtcgg aagcagcggg ccagagcatt
     8401 ttttcctcaa ccttcgtcta tcctcaatga acttggaagg tattccatcc ctcggattga
     8461 ttttgcgatt ttatccgcta gttttcactt tgtttctgtt cgaggaagtc tatgcatgca
     8521 gggaaaatag acatcttcgg attctgcttt ggtgctttat gattttatat gccatggcta
     8581 tgatgggcaa atcatcgata ttgacaccat ttttttcatg ggcaattatt caaggaataa
     8641 agggcagatt gaaagctaaa atgatcgtaa tattggtact agttgtattt gcatcgatga
     8701 tatccttgca cgtcatccgt tcatctactt cttatgtttt ctcaactgtg gatatattag
     8761 ctatttatat atattcccca ttggtagcat taggttatat gaatatcgat agctcactgc
     8821 cggtcggtgc atacgtattt cgtttcttct atgtaatagg taatattctt gatattgcat
     8881 cgcatcctgc aagtacaatc accccctatg ttgcagttcc agagttaaca aatgtttaca
     8941 ctgtcatgca accattttac cacgacttcg ggatgttagg cgttctttta gaagcagtca
     9001 tttatgggct ttttttctca attttatatt ttctttctgt aaagagaggt ggattttggt
     9061 tgattttgtt ttcaggctat tctatagttt tggtgatgca attttttgcc gatctcttaa
     9121 tctctcggtt atctcttaat ttgcaattac tgctttatgc tttaatcatt ttttggactt
     9181 ctaggaaggt tcaatatgcc agatagagaa ttgcagataa ggtccattaa aaactcgata
     9241 tctaaaagag gtacttttgc atggtttccg tagcagagtc acagatgaag caagtcgagg
     9301 ttctactagc cacatacaac ggttcgcaat acgtagtacc tctcatgaat agtcttctca
     9361 accaagatta taaaaatata aaaatcattg tgagagatga tgggtccaat gataataccc
     9421 tagaagttct taataggtat acatcatatg agaatgtgtc aataattgag ggagagcatg
     9481 aaggagttcc ggagagtttt tttagcttgc tccaattgtc ctcatcagat tcgcattatt
     9541 ttgctttcag cgatcaggat gatgtttggc aaagagataa aatatctcgt gcaataggtt
     9601 tcttagggag attgcctcag gaattgcctt caatgtactg ctccagagtc ttgatagttg
     9661 atgaaatgtt aaatccaatt gactattctc caatcccaaa acgtgcacca tcttttgaaa
     9721 atgcaatcgt cgagaatatt gcaacaggat gcacgattgt gataaataaa gctgctaggg
     9781 atttgctatt gaataatagt cctaagaatg ctttaatgca tgattggtgg acatatttgg
     9841 tggtctcagc cttcggagaa gtattttatg atcctgagcc aagaattcta tatagacagc
     9901 attcatccaa tgttgttggc tgcaaaatag gatttatcaa taggtggagt tcaagagttg
     9961 aaagatttct caaatacagc agcctgcgtc ggcccaccag gcaagcaatg gagtttgacg
    10021 agatttatgg cgctagcctg cccaaagata aaaagctggt attggataga tttattgatg
    10081 ggagaaacac ccatatgggc cgagtgctat atgcccttaa cggtgaaata tatcgccaat
    10141 cgattataga tagtattatt ttgcgggtat taatcctatt gaaccgtatc tagactcgga
    10201 tttctctgtt tgccagtgga tatgaaaatg gaatggaagt ccttatagag tggtagccta
    10261 aatctagctt tggatgtgta ttaatctatg attacaggat ctatctgcca gacatgtctc
    10321 ataaattgaa ttcccgcata ataatatgcg ttataggaaa tcaggccttc tcactattga
    10381 acttccgagg atcacttata gcagagatgg ttgcaggagg cgctaaagtc ttggccctag
    10441 ccccggacta tgacgagcac acaagggcag aggtgcgtgc ttttggtgca gatcccgtag
    10501 acttttctct ctctcgcaca ggcatgaatc caattaggga cgttaaagat atgctgcgtt
    10561 tggcctttct cctgagaaag ctgaagcctg atatctccct aggctatgcc attaagcctg
    10621 tgatcttcgg aactcttgcc gcttggctgg ctagggtacc gcggcggttt gccatgattg
    10681 aggggcttgg ctacatattc actccggcag aaggggcaga tcaatttaag cgtcgagttt
    10741 taaaggggat tgcaatcatg ctctacgcta tggcactcaa gcctgcaaac ctggtctttt
    10801 ttctgaataa agatgatatt gatgaattct caaaaaaacg gattatatct cctaaaaagg
    10861 cctttctatt gggtggaatc ggagttgatc taaatgagtg ggtatcaact tcacaagtca
    10921 tgaagcctgt gacatttcta ctggcggcgc ggttgctgag agaaaaaggg gtaatggagt
    10981 atgcgaaggc tgccagactt atcaagcaaa agcatcccaa caccaggttc gttcttttgg
    11041 gcgatatcga gaccaatccc ggttcgctat cctgtgatct gattaaagca tgggatgcag
    11101 aaggaattct ggaatggcta ggtcatgtcc cagatgtgag attttggatg gaaagagcaa
    11161 gcgtatttgt gctaccatcc tactatcgcg agggtgtacc ccgcagcatc caagaagcta
    11221 tggccatggc ccgccctata atcaccactg atgctcctgg ctgcagggat actgttattg
    11281 atggcctaaa cggcttcctc gtgcctgtgc gtgatgtctg cgcacttgct gattcaatgg
    11341 agcgatttat attgcagccc gaactaattg atgagatggg aaaggctagc agatccttag
    11401 cagaagaacg ttttgatgtg cacaaggcaa acaagatcat tctacatgag atgggtttag
    11461 atgagttaaa cattatttaa agtatattta aaatatatta tttaataatt ttaaaattgg
    11521 gcttttaaca atagtaatct ctttccttgg tccaatttag caaaatcctc ataaggtgaa
    11581 attgtctcca aagcttattt atagatataa ttaaaattct cctttggagt gaaagtcctt
    11641 tatgaatgaa gcgaataatc gctatgtttg gacaaaatta gctctcctat tgataatatt
    11701 accgatattt attggattag tatgcgtaat caaatttggc gttaatgttc cctactggga
    11761 tgaatgggac atccagacca aaactttaaa aaattattat gaagatagtt tatctctaaa
    11821 tgatctattt tcccaacata acgaatctag gccctttttc ccacgaatag ttttgcttgt
    11881 tattgattta attacaaatt ataatatcgt agctgaaatg ataatattat ttctaatata
    11941 ttgtttgtca ttcttaatta tattcttgat gtttagaaaa gataatttag gaaataaatc
    12001 atacctctgg ctatttat
//
LOCUS       DAOSGP010000010        11801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_4585_length_11801_cov_19.834412, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000010 DAOSGP010000000
VERSION     DAOSGP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4585_length_11801_cov_19.834412"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..573)
                     /locus_tag="PLV49_00680"
     CDS             complement(109..573)
                     /locus_tag="PLV49_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HOG97385.1"
                     /translation="MARVAVGGTFDPIHDGHLALLRRAFELSGDGEVVIGLTSDEMAR
                     SSRTRSVRKYEVREKNLRAVIKKCFGIDDVHITKITDQCGPSIYESFDFIVVSPETLP
                     MAEKINRLRAKKNLPPLQISEIEYQMAQDKVRISSTRISEGKIDRHGRVLVG"
     gene            complement(615..1046)
                     /locus_tag="PLV49_00685"
     CDS             complement(615..1046)
                     /locus_tag="PLV49_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
                     /protein_id="HOG97386.1"
                     /translation="MTLVVATGTFDLLHPGHVLYLERSKALGDELVVIVARDVNVRHK
                     AKPILPEEQRRRMIESLKPVDRAILGEVGDIFRTIEDLRPDIITLGFDQHFDPLRLEH
                     DLFRRGLHPRIVRIEEQEPCELCSSRRIVNKILERFRGRRI"
     gene            complement(1081..1497)
                     /locus_tag="PLV49_00690"
     CDS             complement(1081..1497)
                     /locus_tag="PLV49_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HOG97387.1"
                     /translation="MRRKRIAGIARDTLNFILEASRSTAPSEFAGLLQADGEIITEVL
                     ILPGTESSRMSALVRLYMLPNMQVAGSVHSHPSGALRPSEPDLLFFSRTGDYHIIVGP
                     PFDEQSWVCYNASGVRRDLPILDVKFDDDDFDDDLD"
     gene            complement(1494..2363)
                     /locus_tag="PLV49_00695"
     CDS             complement(1494..2363)
                     /locus_tag="PLV49_00695"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG97388.1"
                     /translation="MAAYGGDASSHEARRRSELSYGPHLVDRELAPLDKDRILSCLGT
                     RWLGRDLRLFAQLASTNELAISLAQKGESGTVILAEVQTRGRGRLSRHWESPSGGIWM
                     SLILRPDIPIALAYQINMAICVAVCRAISNLLGLNPGIKWPNDLLIGERKVGGILMEV
                     QAEGERLEYAVVGVGINANIDPSAFPDDWMATSLSQEGGSEVSRTALIQRTLLEIERA
                     YENLGSMEIYDEWRRRSVTIGRMVRISANSGELVGEVEDLAEDGALLLRRDDELVRVL
                     AGDCIHLRGMGGL"
     gene            complement(2366..>3330)
                     /locus_tag="PLV49_00700"
     CDS             complement(2366..>3330)
                     /locus_tag="PLV49_00700"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719188.1"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOG97389.1"
                     /translation="EEMLPALTSSQSVARNAFGDDTIYIEKYLTEPRHIEFQILADEL
                     GKTIYISDRECSIQRRHQKLIEESPSPVMSDELRERMGSIAVKAAKAIGYASAGTVEF
                     MYSRGDFYFLEMNTRLQVEHPITEMVTGVDLAKEQILIAAGQPLNYDQSDMQIRGWAI
                     ECRINAEDPLNDFSPAPGRIKGYRSPGGPGIRVDSGVYSGYLIPPYYDSLISKLVAHG
                     KDRREAIARMERALYEYIIVGVKTNLIFHKAVLRNARFRSGDFNTGFIKEENIAEAVK
                     RVAAEDYDRNQSLASALGADNRKVAAIAAAVGSYLSQQKLEEKS"
     assembly_gap    3331..3430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3431..3729)
                     /locus_tag="PLV49_00705"
     CDS             complement(<3431..3729)
                     /locus_tag="PLV49_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOG97390.1"
                     /translation="MFNKILIANRGEIAIRVMRACRELGIPSVAVYSEADKNALFAKY
                     ADEAVYIGPSPSSQSYLKIDNIIKAALETGAEAIHPGYGFLSENTVFASTCKENG"
     gene            3804..4232
                     /locus_tag="PLV49_00710"
     CDS             3804..4232
                     /locus_tag="PLV49_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG97391.1"
                     /translation="MKPRELSDDECIALLSSARYGRLGLSREDQPYVVPMSFVYHEGK
                     IYLHSRGMGRKVQYAAENPRICFQVDHLEKGKWTSVNAFGRAELSDDIEAKQRMFDAF
                     TKKGLGGHGGKSFQRQDLERMDMTIWEIVIDELTGREGVW"
     gene            4233..>5435
                     /locus_tag="PLV49_00715"
     CDS             4233..>5435
                     /locus_tag="PLV49_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HOG97392.1"
                     /translation="MPAPLKWTEKYRPASLKQVLGNGKAIDDLRVWALSWERGEPITG
                     AVILYGPAGTGKTSAALALAHDFDWDYIEMNASDARTAGMIEKIAVPASRSQTFSGKP
                     RLVILDEADNLHGTADRGGAAAMLRLVRNTLQPVILIANEYYEIDKSLRDATRGIQFR
                     SVRSTTIAQALRAICQAEGIDCDPDLLVTIAERAGGDMRSGINDLQAAAEGQETLREE
                     DLATAQRDVKSSIFRVLEVIYKGNNAKEALEASYNLDESPEDLINWVDENLPLAYSGQ
                     DLLRGFETLARSDIFLGRVRRRQNYGLWRYASYLMTGGVQAAKKERRHGYVAFRPPSL
                     WRRMGQTRKARSIRDSAARKIGRHCHVSLAFARSELMDFVGMLLKDKKGAPQLAARLD
                     LTAEEIALL"
     assembly_gap    5436..5535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5668..6366
                     /gene="gvpD"
                     /locus_tag="PLV49_00720"
     CDS             5668..6366
                     /gene="gvpD"
                     /locus_tag="PLV49_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD"
                     /protein_id="HOG97393.1"
                     /translation="MKSGIPGFDEMVGGGFHQGTVNTITGSSGTGKTVFASQFIHHGI
                     GQGEKGMIITPSESAEYMRREMMSSFGWDFGALEKDGKLSLIDVTDPVLRLQKSIDVD
                     PVDFLINFRKLVERKLHEEKPKRLFIDDLTAFFMAVEAPFKIRSLTDDLFGTIRSSGV
                     TCLVTIGTAFGTNQFLEYGADSATMLNRERIGNTLIRSIYVMKMRGSKIANSIRVLDI
                     SDDGISVSTLSPYP"
     gene            6374..8158
                     /locus_tag="PLV49_00725"
     CDS             6374..8158
                     /locus_tag="PLV49_00725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG97394.1"
                     /translation="MIERWIILAAEEAGPVSNKMGGIWNVVDAEATTLARLVAKGELD
                     SEFRILVVGPNYPTAGSDWNTGRNRVTDISALNRLDMGEELALTLAELNSLGMESVTG
                     QRVEESVPIGYVLFNTNYYQSRLVRWKDREMTLNNAIKTEAFDLLGLDSMRFEKEHYG
                     WEYNHYLNLSYAVSELVRILAQSPEESTKKYADKAVVEFARSVMPRVRVSLHCHEFGI
                     FYAAARLEKLGIPVRTLCTFHATVPGRTAGYRSLEKIRKNDSKMEPGTPLGFAALEAL
                     ARYADGVTFVGDSTMKEAMLFYRMKGVVIRNGIDVRGDRIDWNKKERCRARIQDFIIN
                     NLYKNCEGSCPERENIIPVFSISRIEIENKGYPQLLDALVLQDHLLRHRMMGQRHADQ
                     TRVVCFLIAAHGPKSKDKLPEGFPVNLPIEVLLGDEIRLENMINDRRLNKDELISGRR
                     SVAAVLYPQWLGPNDGGLGMRTDEVMAGCVAGIFPSQYEPFLLTALEAGREGTPSIVS
                     RSAGFSDALKKVEHRVPGLGGVVVVDNIELQPQETVMDYALALDYFSRTYLEDDVKYR
                     LLCEESFSLARQFSWDQPVMEYYKNLTV"
     gene            8223..9461
                     /locus_tag="PLV49_00730"
     CDS             8223..9461
                     /locus_tag="PLV49_00730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG97395.1"
                     /translation="MRIAYFSTEYPPLVYGGLGVYADNISRSLVALGQKVTVFSWGSK
                     ESPRHEMLVGVDVFREMPLAMRDGMEIFLSPQTLRWGQGLDYLLNLFSYNQLALDHLK
                     EEDRFDLLVSHDWLALPGGMAARREGTPLIYHVHGLETGRSSSPNPQIVALEHKGSEV
                     ADLVITVSEAMKRELAGQGISEDKIRVCYHGVDCEFFDPEKVDRESLVALRERYGFDG
                     DDVVILFVGRLEPVKGIRELFSALPSVQSEYNRVKLLVLGVGSLEGWAKIEAERQSNI
                     TLVTDFVDAEEKRLHYALADLCVFPSLYEPFGIVALEAAAMGKAAVVGAAGTSGLAEI
                     VKNPAEDEPTGVHVNARDPADLAWGINLALEDKERLLSWGKNARKRAQISFSWQKAAE
                     STLAIYEEAIALFRKRSEGL"
     gene            9464..10447
                     /locus_tag="PLV49_00735"
     CDS             9464..10447
                     /locus_tag="PLV49_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HOG97396.1"
                     /translation="MPEIRRHYFLEEYCIIAAERNKRPSDFLVAKAVPGDEKDCPFCP
                     GHEDRTPPTIAAYTDDGILLQDETGSELWQIRVFLNAYPTMIPRPEPPAAEWISLPGQ
                     GYHEVIVDSREHRKNPRDFGKKHLDMLVSAFTDRYRHYSTHEDVKYITIFKNWGKESG
                     ASLSHSHSQLITLPFLPPQMKREEEAISRSSFCPYCNIVDRESASCRLINENDHWILF
                     APFYSLMPYETWILPKRHLGNLTDLRDEEQKSLASILAELLRILYSTLDDPPYNLMIF
                     QLPKEYHFNIRIQPIVSRIAGFERSSGIYVNPVPPELAAAELKNFCLPPKD"
     assembly_gap    10566..10665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10690..>11801
                     /locus_tag="PLV49_00740"
     CDS             10690..>11801
                     /locus_tag="PLV49_00740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG97397.1"
                     /translation="MRIGMLSWESLYSIKVGGVAPHVSELSEALARRGHEVHIFTRRG
                     DFESYDKINGVHYQRADVDDHGDIVDQMNRMCDALYHRFGAVQQLFGNFDVVHGHDWH
                     PVLALTRIKSDYHLPFLLTMHSTEWGRNGNTFGYGISEEISHREWQGCYEAAKVIVTT
                     RRMQDELMQIYSLPKDKIPIIPNGIIVGKMRRGVDAGRIKEKYGIHPLAPVVLFCGRM
                     SVQKGPDLLVEAIPNVLRNRPDVCFVFIGEGGMRSECEQKARYLGVADSCRFLGYTSS
                     ADKQMLINACDMMCVPSRNEPFGVVVLEAWDACKPVVATDAVSIINNFQDGLLAYIQP
                     ESIAWCINRIISNPKEMKHLARAGWTRIESEFSWDH"
BASE COUNT         2700 a         3078 c         3205 g         2518 t
ORIGIN      
        1 ggaagacctg cattggagtg cccaccaggc tgatctactc tctgaagagc tggatcgata
       61 agtcatttat caagagattt aagtaaaata tagtgtttca atccgtcttc agccaactaa
      121 gacccgacca tgccggtcga tctttccctc ggatatcctg gtggagctta tccggacctt
      181 atcctgggcc atctgatact ctatctctga tatctgcagg ggcggcaggt tcttcttggc
      241 ccggaggcgg tttatcttct ccgccatagg cagggtctca ggggatacca caataaaatc
      301 gaagctctca tagatcgatg gaccgcattg gtctgtgatc ttcgtgatgt gaacatcgtc
      361 aatcccaaag catttcttaa ttacagcgcg caggttcttc tctcgtacct catatttgcg
      421 caccgatctg gtcctgctcg acctggccat ctcgtctgag gttaggccta taaccacctc
      481 tccatctcca ctcaactcaa aagcgcgccg gagcagggct aaatgaccgt catgaatggg
      541 atcgaaggtg cctcctaccg cgactctggc catacaggcc cgcaaactat cagtgccgta
      601 taagtacttg gcggctagat gcgacgaccc ctgaatcgtt caaggatctt gttgacgata
      661 cgcctggagc tgcacagctc acagggctcc tgctcctcta ttctcacaat cctgggatgc
      721 aggccgcgac ggaagagatc atgctccagg cgtaagggat cgaagtgctg gtcaaagccc
      781 agggtgatta tgtccggccg gaggtcctct atggtccgga agatatcgcc cacctcgccc
      841 agaatcgctc tatccaccgg ctttaaggat tctatcatcc tccgcctctg ctcctcgggc
      901 aggatgggtt tggccttatg gcgcacattt acatccctgg cgactatcac caccagctca
      961 tctcccaggg cttttgatcg ctccagataa aggacatgcc cgggatggag aaggtcgaag
     1021 gtgccggtgg caactaccag cgtcactgct caatctccgt tcccggcttg tgccgcctct
     1081 tcaatcgaga tcatcatcaa agtcatcgtc atcgaacttt acatccagga tgggaaggtc
     1141 tcgcctcact cctgatgcat tatagcagac ccagctctgc tcatcgaacg gcgggccgac
     1201 gatgatatgg tagtcgccgg tgcgagagaa gaacaggaga tccggctctg aggggcggag
     1261 cgctcctgag ggatggctgt gaacagaacc cgcaacctgc atattgggaa gcatatagag
     1321 ccggaccaga gcgctcatgc gggagctctc tgtgccaggc agtatcagaa cctcggttat
     1381 gatctcacca tccgcctgga gaagtccggc gaactccgac ggggctgtgg accgcgatgc
     1441 ctccagtata aaattcagag tgtctctggc aatgccagct atccgcttcc ttctcatagt
     1501 cctcccattc cccgcagatg gatacagtcc cctgccagaa ctcgaaccag ctcgtcatcc
     1561 ctcctcaaca ataaggcccc atcttccgcc aggtcctcca cctcacccac cagctctcca
     1621 gagtttgcag agatgcgcac catcctgcca atggttacgg agcggcggcg ccactcatca
     1681 tagatctcca tcgatcccaa attctcatat gccctctcta tctccaggag agttctctgg
     1741 atcagagcag tgcgagagac ctcagatcca ccctcttggg acaaagatgt cgccatccag
     1801 tcatctggga aagcggacgg atcgatattg gcgttgatgc ccacacccac aacggcatat
     1861 tccagccgtt ccccctctgc ttgaacctcc atcaatatgc ctcccacctt cctctccccg
     1921 atcagaaggt cgttgggcca cttgattccc ggattgaggc cgagcaaatt ggatatggct
     1981 ctgcagacgg caacgcagat tgccatattg atctgatagg cgagagctat ggggatatcc
     2041 ggcctcagaa tgaggctcat ccagattccg cctgatggcg attcccagtg tctggagagc
     2101 ctgccccgcc ccctggtctg aacctcagcc aagatgactg tgccactctc gcccttttga
     2161 gccagcgaaa tggccaattc attggtggag gcaagctggg caaagaggcg caagtccctt
     2221 cccagccacc tggtaccgag gcaggagagg atccgatctt tgtccagtgg agccaactct
     2281 ctatccacga gatgagggcc gtaactcagc tcggacctcc ggcgggcctc atgggatgat
     2341 gcatcgcccc cgtaagcagc cactatcagc tcttctcctc cagcttctgc tggctgagat
     2401 atgagcctac cgcggcggcg atcgctgcca ctttgcggtt atcggcccca agagcagaag
     2461 ccaaagactg attccgatca taatcctctg ctgctaccct cttcaccgct tcggcgatgt
     2521 tctcctcctt gatgaagccg gtattgaagt caccacttct gaacctggca tttctgagca
     2581 ctgccttatg gaagatgagg ttggtcttaa cccccacgat gatatactca tagagggccc
     2641 gctccattct ggcaattgct tctctacggt ccttgccatg ggcgacgagc ttggagatca
     2701 aggaatcgta gtaaggagga atcaggtagc cggaatatac cccgctgtcc accctgatcc
     2761 ccggaccgcc gggactcctg tatcccttga tcctcccagg agcaggagag aaatcgttta
     2821 aggggtcttc tgcatttatt cggcactcta ttgcccagcc ccgtatctgc atgtcgctct
     2881 gatcgtagtt caggggctgg ccggcggcga tgaggatctg ctctttggcc aggtccaccc
     2941 cggtcaccat ctcggtgatg ggatgctcaa cctgcaggcg ggtgttcatc tccaggaagt
     3001 agaagtcccc ccgggagtac atgaactcca ccgtccctgc gctggcatat ccaatggctt
     3061 tggccgcttt gacggcgatg gagcccatcc tctcgcgcag ctcatcgctc atcaccgggg
     3121 agggagactc ctctatcagc ttctgatggc ggcgctgaat ggagcactcc cgatcggata
     3181 tgtatatcgt ctttcccagc tcatccgcca ggatctggaa ctcgatatgc cttggctctg
     3241 tgaggtactt ttcaatatat atggtatcat cgccgaaggc attgcgggcc acagactggg
     3301 atgaggtcag agcgggcagc atctcctccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn atgccgtttt ccttgcaggt ggaggcgaag acggtgttct cggataaaaa
     3481 gccatatcca ggatggatag cctcggcacc ggtctccagg gcggccttga ttatgttgtc
     3541 tatcttcagg tagctctggc tggatggaga tggtcctata tagaccgctt catcagcgta
     3601 cttggcgaag agggcatttt tgtccgcctc ggagtagacg gccacgctcg ggatgcccag
     3661 ctcccggcag gctcgcataa cccggatggc gatctctccc cggttggcta tcaggatctt
     3721 gttgaacatc cgcatatgaa agactctgcc agggtaaaac ctttttggca aaagataaaa
     3781 acctggagga tcaaatgcag atcatgaaac ccagggagct atcagatgat gaatgcatag
     3841 cactcctctc ctcagccaga tacggcaggc taggactatc cagagaagac cagccttatg
     3901 tggttccaat gtcctttgtg taccacgagg gcaaaatcta ccttcactcc cgagggatgg
     3961 gaagaaaggt ccagtatgca gctgaaaatc cccggatctg ctttcaggta gaccatctgg
     4021 agaagggaaa atggacgagc gtcaatgcct tcggcagggc agagctatcg gatgatatag
     4081 aagccaagca gaggatgttt gatgccttca ccaaaaaggg tttgggcggc catggaggca
     4141 agagctttca acgccaggac ctggagagga tggatatgac catatgggag attgtcatcg
     4201 atgagctgac gggaagagag ggcgtctggt agatgcccgc acctttaaag tggacggaga
     4261 agtatcgtcc agcctccctc aagcaggttc ttggcaatgg caaggcgata gatgatttgc
     4321 gtgtgtgggc actctcctgg gagagaggcg agcccatcac cggcgcggtc attctctacg
     4381 gcccggcggg aacgggaaag acttcggcgg ctttagccct ggcacatgat ttcgattggg
     4441 attacattga gatgaatgcc tcagatgctc gcactgcagg catgatcgag aagattgccg
     4501 ttccagcatc gcgaagtcag acattctccg gaaaaccgag gctggttatt ttagatgaag
     4561 cagacaacct ccatggcact gccgaccgag gcggagcagc ggccatgctc cgcctggtca
     4621 ggaatactct ccagccagtg atactaattg ccaatgagta ctatgagatc gacaagtccc
     4681 tccgagatgc gacccgagga attcagttcc ggtcggtgcg ctcaaccacc atcgcccagg
     4741 cattgagggc gatctgccag gctgagggca tagactgcga tcccgatctg ctggttacga
     4801 ttgccgagag ggcaggggga gacatgcgct ccgggatcaa cgatctgcag gcagccgccg
     4861 aaggccagga gacgcttagg gaagaggatc tggccactgc ccagagggat gtcaaatcct
     4921 ccatcttccg ggtgctggag gtgatctaca agggcaataa cgccaaggag gccctggaag
     4981 cgtcctataa tctggacgag agcccggagg atctgatcaa ctgggtggat gagaacctgc
     5041 ccctggcata ctccggccag gatctcctcc ggggcttcga gaccctggct cgaagcgaca
     5101 tatttctggg cagggtacgg cggaggcaga actacggcct gtggcgctat gccagctatc
     5161 tcatgaccgg cggagtgcag gcggcaaaga aggagcgcag gcatggttat gtggccttcc
     5221 gtccacccag cctgtggcga aggatgggcc agacccgcaa agccagatcg ataagggact
     5281 ctgccgccag aaagatcggc aggcactgcc atgtatcgct cgccttcgcc cgctcggagc
     5341 tgatggactt tgtgggaatg ctgctcaaag acaagaaagg cgctccccaa ctggccgccc
     5401 ggctggatct gaccgcagag gagatcgctc tgctcnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnntaacc agcaagctta accgaaaggg ctaactagca taattgagaa
     5581 aataaactgc agcgggcgct gcaaaccaaa gcgcaagagc tgcaaggggt gttaattatg
     5641 gcagtacaag gcagcaaatt tcctagaatg aaatccggca tacctggttt tgatgaaatg
     5701 gtaggtggcg gatttcatca gggaactgta aataccataa ccggttcctc aggcaccggc
     5761 aagactgttt tcgccagcca gttcattcac cacggcatcg gacaggggga gaagggaatg
     5821 atcatcactc cctctgagag cgcagaatat atgagacggg agatgatgtc ctcctttggc
     5881 tgggattttg gcgcattgga aaaagatggg aagctgagcc tcatagacgt tacagacccg
     5941 gttctccggc tgcagaagag catcgatgtc gatccggtgg attttctcat caacttcaga
     6001 aagcttgtgg agagaaagct gcatgaagaa aagcctaaaa ggctattcat cgacgatctg
     6061 actgcatttt ttatggcggt agaagcgccc ttcaagatcc gttcgctgac ggatgacctc
     6121 ttcggcacca tccgttccag cggagtgacc tgcttggtca ccataggcac cgcttttggc
     6181 accaatcagt tcctggagta tggagcagac tccgccacca tgctgaaccg ggagagaata
     6241 ggcaatacgc tcatcagatc gatctatgta atgaagatga ggggctcgaa gatcgctaat
     6301 agcatccggg tgctggacat atcggacgat ggcatctccg tatcaacgct ctctccttat
     6361 ccatgaggtt agaatgatag aaaggtggat catcctggca gcagaggaag cgggccccgt
     6421 ctccaataag atgggaggca tatggaatgt ggtggatgcc gaggccacga ccctcgcccg
     6481 gctggtggct aaaggggaat tagatagcga atttcgtatc ctagtggtcg gccccaacta
     6541 tcccactgcc ggttcggact ggaataccgg ccgaaaccgg gtgacggaca tatcggcact
     6601 aaaccggctg gacatgggtg aggagctcgc cctaaccctg gcggagttga actccctggg
     6661 aatggagagc gtcaccggcc aacgggtcga ggaaagcgtt cccatcggct atgttctgtt
     6721 taataccaat tactatcagt ctcgattggt gcgctggaag gaccgagaga tgacattgaa
     6781 taatgccatt aagacagagg ccttcgatct attgggactg gactcaatga gatttgagaa
     6841 ggagcactat ggctgggagt ataatcacta tttaaacctc tcctatgcgg tctcggagct
     6901 ggtgcgtatc ctcgctcagt cccctgagga gagcacaaag aagtatgcag ataaggcagt
     6961 ggtcgagttt gcccggtcgg ttatgcccag ggtgagggtc tcactgcact gccatgagtt
     7021 tgggatcttc tatgccgccg ccaggctgga gaagctgggc attccggtaa gaactctctg
     7081 caccttccat gcaaccgttc ccggcaggac ggccggctac cggtcgctgg agaagatcag
     7141 aaagaatgac tcaaaaatgg aacccggaac ccctctgggc tttgccgctc tggaggcgct
     7201 ggcccgctat gccgatgggg tcacattcgt gggcgactcg acgatgaagg aggcaatgct
     7261 cttttaccgc atgaaagggg tcgttattcg caatggaatc gatgttaggg gcgatcgaat
     7321 cgattggaat aagaaggagc gctgccgggc acggattcag gatttcatca tcaataacct
     7381 ctataagaac tgcgagggca gctgcccgga gagggaaaat atcattcccg tattctccat
     7441 ttccaggata gagatagaga acaaaggtta tcctcagctt ctcgatgccc tggtgcttca
     7501 ggatcatctc ctgcggcaca gaatgatggg acagcgacat gcagatcaga ctcgggtggt
     7561 atgcttcctc atcgccgccc atgggcccaa atccaaggat aagcttcccg agggcttccc
     7621 cgtcaacctt cccatagagg ttctgttggg cgatgagatc cgcctggaga atatgatcaa
     7681 tgatcgacgg ctcaacaagg atgaactgat ctccggccgg aggtcggtgg ctgccgttct
     7741 gtatccccaa tggctgggac cgaatgacgg cggcctggga atgagaacgg acgaggtcat
     7801 ggccggctgc gtcgctggca tcttcccctc ccagtacgaa ccctttcttc tcaccgcatt
     7861 ggaggcggga agggaaggaa cccccagcat cgtcagccgg agcgcaggat tcagcgatgc
     7921 cctgaaaaag gtcgagcatc gggtgccagg gctgggtggg gtcgtagttg tggacaacat
     7981 cgaacttcag cctcaggaga cggttatgga ctatgccctg gctctggatt acttctcccg
     8041 gacctatctg gaggacgatg tgaaataccg gctgctgtgc gaggagtcgt tctccctggc
     8101 caggcagttc agttgggacc agcccgtaat ggaatattat aaaaatctga ccgtctgacc
     8161 tcggatataa ttccggtctg aattaaataa acaatctttg ggagtgacat cgattgaatt
     8221 caatgcgaat cgcctatttc agcactgagt acccccccct agtctatggt gggctggggg
     8281 tctatgcaga taacatatct cgctctttgg ttgcccttgg ccagaaggtc accgtcttct
     8341 cctggggttc gaaagagtct cccaggcatg agatgctggt cggggtggat gtcttccgag
     8401 agatgcccct tgcgatgagg gacggaatgg agatctttct ctccccccag accttgcggt
     8461 ggggtcaggg cctggattat ctgctcaacc tgttcagcta caaccagctc gctcttgacc
     8521 atctgaaaga agaggatcgg tttgatctgc tcgtctccca tgactggctg gccctcccag
     8581 gtggcatggc cgccaggaga gaagggactc cactgattta tcatgtccac ggcctggaga
     8641 ccggccggtc ctcaagtccg aacccgcaaa tcgtggcttt agagcacaag ggctcagagg
     8701 tggctgacct ggtgataacc gtatccgagg ccatgaaaag ggagctggcc ggtcagggga
     8761 tttctgagga caaaatccgg gtctgctatc atggagtgga ctgcgagttc ttcgatcctg
     8821 aaaaggtgga tcgagagtca ctggttgctc tgagagagag atacggcttt gatggggatg
     8881 atgtcgtaat tctcttcgta ggacggctgg agccggtcaa aggtatcaga gagcttttct
     8941 ccgccctgcc cagtgttcag tcggagtaca atagggttaa gctcctggtg ctgggggtcg
     9001 gcagcctgga gggatgggcc aaaatagagg cggagcggca gagcaatata actctcgtca
     9061 ccgatttcgt cgatgctgag gagaagaggc ttcactatgc ccttgccgac ctatgcgtat
     9121 ttcccagcct ctatgagccg ttcggaatag tggcccttga ggcagcggcc atgggcaagg
     9181 cggctgtggt aggggcggca ggcaccagcg gcctggcgga gatcgtcaag aatccggctg
     9241 aagatgagcc gacaggggtt catgttaatg cccgcgatcc agccgatctg gcctggggaa
     9301 tcaacctcgc cctggaggat aaagaacgtc tcctgtcctg gggaaagaac gccagaaaga
     9361 gagcccaaat ctccttcagc tggcagaagg cggcagagag caccctggcc atctacgagg
     9421 aggcaatagc gcttttcaga aagagatcag agggattata ggcatgcccg agatcagacg
     9481 gcactatttt ctggaagagt actgcatcat cgccgccgaa cgcaacaaaa ggccctcaga
     9541 tttcctggtg gccaaggcgg tcccaggaga cgaaaaggac tgcccttttt gcccgggcca
     9601 cgaggatagg actccgccca ccatcgctgc ctacaccgac gatggcatcc tgctccagga
     9661 tgaaactgga tcagagctct ggcagatcag agtgtttctc aatgcttatc ccaccatgat
     9721 ccccaggccc gagcctccgg cggcagaatg gatatccctg cccggccagg gctatcacga
     9781 ggtgattgtg gattcgagag agcaccgcaa aaatccgagg gatttcggca aaaaacacct
     9841 ggatatgctc gtttcggcct tcaccgatcg ttaccgacac tattccaccc atgaggatgt
     9901 gaaatatatc accatcttca aaaactgggg caaggaatcg ggggcctcgc tatcccacag
     9961 ccattctcag ctcatcaccc ttccctttct acccccgcag atgaagagag aggaggaggc
    10021 catatccagg tcatccttct gcccttactg caatatcgtg gaccgagaga gtgcgtcatg
    10081 ccggctcatc aatgaaaacg atcactggat cctatttgca cccttctact ctcttatgcc
    10141 ctatgagacc tggattcttc ctaaaaggca tttgggaaac ctgacggatt tgagggacga
    10201 agaacaaaaa agcctggcca gtatactggc agaactcctt cggatcctct attccaccct
    10261 ggatgatcca ccctacaacc tgatgatctt ccagcttcca aaagagtatc acttcaacat
    10321 caggatccag cccatagtct ccaggatagc aggctttgag aggagttcag gcatatatgt
    10381 gaaccccgtt cccccggaat tggcagcagc agaattgaaa aatttctgcc tgccgccgaa
    10441 agattaatga attatcggac ttaagtgatt taaggattag atcagcctga aatgctttta
    10501 aacaggcaaa caataagaaa cgacagcaaa aaaatggtcg tactgataat aatttggcat
    10561 cctttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccttt agggatgaat
    10681 ggtgaataga tgcgaattgg aatgctctcc tgggagagtc tgtattcaat aaaagtaggt
    10741 ggcgttgccc ctcatgtctc agagctatcg gaagcgctgg ccaggcgggg ccatgaggtc
    10801 catattttta cccgtagagg ggactttgag tcctatgaca agataaatgg ggtgcactac
    10861 cagcgggctg atgtagatga ccatggcgat atcgtcgatc agatgaaccg gatgtgcgat
    10921 gccctctatc atcgtttcgg tgcggtgcaa cagctcttcg gcaacttcga tgtagtgcac
    10981 ggccatgact ggcatcccgt cctcgccctg acccgaataa agagcgacta tcatctaccc
    11041 ttcctgctga ccatgcacag cactgaatgg ggtaggaacg ggaacacctt tggatacggc
    11101 atatccgagg agatatctca tcgagagtgg cagggatgct atgaggcggc taaggttatt
    11161 gtgaccacca ggaggatgca ggacgagctg atgcagatct acagcctgcc caaggacaag
    11221 attcccatca ttccaaatgg cattatcgtg ggcaagatga ggagaggcgt agacgccggc
    11281 cggatcaagg agaagtacgg catccatccc ctggcccctg tggtgctctt ctgcggcagg
    11341 atgagcgtcc agaagggtcc agacctcctg gtagaggcga tacccaatgt gctgagaaat
    11401 agaccggatg tctgcttcgt cttcataggt gagggtggca tgagatcgga atgcgagcaa
    11461 aaggctcgct atctgggcgt cgctgattcc tgccggttcc tggggtacac ctccagcgcc
    11521 gataagcaga tgctgatcaa tgcctgcgat atgatgtgcg ttccctctcg caatgagcca
    11581 tttggagtgg tcgtgctgga ggcatgggat gcctgcaagc cggtagtggc cactgatgcg
    11641 gtgagcataa tcaacaactt ccaggatggc ctgctggctt atatccagcc cgagtccata
    11701 gcctggtgca taaaccggat tatcagcaat ccgaaagaga tgaaacacct ggcccgggcc
    11761 ggttggacca ggatagagag cgagttctcc tgggaccaca t
//
LOCUS       DAOSGP010000011        11554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_4692_length_11554_cov_16.677885, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000011 DAOSGP010000000
VERSION     DAOSGP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 11554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11554
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4692_length_11554_cov_16.677885"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1318)
                     /locus_tag="PLV49_00745"
     CDS             complement(86..1318)
                     /locus_tag="PLV49_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG97398.1"
                     /translation="MRTTTNMRLMMTMALCVLFSIQSGIAYNFALDIFGNANMDDTID
                     ESDIAFIREIIGGTQKATAFADANYDGRIDEKDITQIQSIIMGNETNLTIVDGVGRNV
                     TVSVPIENIVTIAGSYGPEMLLALGIKPLAISESKSHAVQLQDMLKDVPTVGSSNEPD
                     SEAILGLNPEIVHCYENFYKQGSYEKMEKALNSRGIGLIAMDFHKPGNFDNAIRIMGY
                     LLGRQERADSLIDFEESNMNLIQERTKNLTDEERTYLYFESSKDFATRGPGDNTYDAI
                     VLCGGRSIFDDIAEPYPTVDPEAIIQRDPHVVIKTPDGFAATNNAEPLIEVLNSLKNR
                     TGWQDLNAVKDGRLYFLNGGTRSVHNSIFCLFLAKALHPDRFNDVDPESIYRQWYNKF
                     LGIDNNLVMVYPPSESWQ"
     gene            complement(1473..2216)
                     /locus_tag="PLV49_00750"
     CDS             complement(1473..2216)
                     /locus_tag="PLV49_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein NifH"
                     /protein_id="HOG97399.1"
                     /translation="MKKIAIYGKGGIGKSTTAANLSAALAKQGRRVMQIGCDPKADST
                     CLLMNGKSVPTILDTLRTKATAVSLEDMVYPGFAGVLCAECGGPTPGVGCAGRGIIAS
                     FEKLEALKAYETYRPDIVLYDVLGDVVCGGFAMPLRRGYADEVYIVTSGEKMSLFAAR
                     NIAQAVGEFKGRGYAQLKGIILNARGIVDELEIVRRAAAEMDTKVVAEIPRDPTIQAC
                     EERNATVIEGAPDTSLAERYRMLARSIVG"
     gene            complement(2213..3523)
                     /locus_tag="PLV49_00755"
     CDS             complement(2213..3523)
                     /locus_tag="PLV49_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOG97400.1"
                     /translation="MDLKHNGFPVPSDYFGVLWALSGIKDLLILEHGSTGTCNFNAFN
                     YMIMNRQSPKGKLFSSGMDEDDVVMGRDEKIREAIVELDRKYHPRIIALVATGVTSMI
                     GLDLKGIIEEIQPTTSARMLSFPSGGFAGDYTTGIREVFGSLARNIAEDCTKEARSND
                     ASSWANPCTVNIIGPTIDSFNIISDLAELERMLGLMGVRVNTVFTCNTDVEAIKNLPC
                     ASLNLVTRDIGLEAAAILKERFGTPYYYGLPFGVKGSIDWMKDIAGILGIGLDKRLVR
                     AELERYGYSILELVNFLQNIDHLRAAISCPFDYSSGLAAMMRDEWGINPSLIVLPASP
                     IGPQRLNREANFGLGKDVRLLIEPDSWTLQDAISSMNPHIVFGNSYDLQISRNVPIRI
                     HAAFPAFDHIYRYDGTPFVGLRGHAYLTQTIVNLLNQNPEVFRN"
     gene            complement(3527..4840)
                     /locus_tag="PLV49_00760"
     CDS             complement(3527..4840)
                     /locus_tag="PLV49_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOG97401.1"
                     /translation="MSIENKCLRLSELPDDGSLAATAHAKYPGQHCSFRAVAGTVPLI
                     KNSYALLFGPAICLYNAKLSMNMRSLTSDPRPNNLLFVNYSQDDIIFGFQEKVRVAIF
                     QADQEYHPEVLFLITSCLQEIVGEDFDACIDEIRPSVKAKLLTIHTENFTCEGAAPGV
                     ENMFKSLFELMKEQPQEERTANILGLWTSTARKTELVRLLSGKGVRIKNVIPSYCTPS
                     ELEEAPGVQLNIVLDQYGLPLARMMKEKFGTNYIYCRKPYHPDSIEEWYQNIASALNI
                     DLSVEVADLKKKTEALREKARAALAGMTCAVSGISGRPFDLALLLTSIGIEPKAMLMQ
                     TIQPEDRPDIQSLLNAGVDPIVFRGDNSLQNEELLAELSPDISVGAFERSSLARMGIR
                     PVILSTSNYLLGFESTLEVMRLLMEGIPGIEALLYKEKILAGGEC"
     gene            complement(4880..8581)
                     /gene="cobN"
                     /locus_tag="PLV49_00765"
     CDS             complement(4880..8581)
                     /gene="cobN"
                     /locus_tag="PLV49_00765"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HOG97402.1"
                     /translation="MAVRSGEDLNDAAQREQFEHFARSCHIAIFNLHGGKKSLSSFDE
                     LVQRLQDSSVSIYAQSASNEPEIELMKLSTVDDAVYRKVSQYLDYGGRENFYNLLLYL
                     ANYFIGSNYEFSEPARPIWEGIYHPDFDHVPTLQEYLQSKCVAGRPTVGLWFYQSLWQ
                     AGNTVFIDRLIEEIEGQGANVIPVFLHAAKDVERGTNGAEWVVENLFMKDGRPIIDVL
                     ISTLMFSLSIKPWEGSDTGEGQEVARSEEWFIKRLNVPVLKAIVTYNTLAGWNESLQG
                     CSPMDISMGIAMPEFDGMLITVPVAARERTDIDPLTGARVIRFEPLPERTNKIVRLSL
                     NWAKLRHIPNSQKKVAIIFHNYPPRDDRIGTAFGLDSPVSVLNIMKAMDDAGYTIERM
                     PENGQALIEDVKSRLTLDRRWRSPEELAKRAIDSVTEDDYRAWFEQLPVAVQEKMTSA
                     WGEAPGKLFVHKKNLIIPGVINGNIFIGLQPPRGFLEDPAAIYHSPDHPIPHHYYAYY
                     RWIRDVFRADLVMHIGKHGSLEWLPGKSVGLSDSCFPDIAISDLPNIYPYIINNPGEG
                     TQAKRRSYCCIIDHLVPVMHNADAYDEMAELEVMLADYYQAASEDPSKLPTQKKMIWE
                     KVCEAKLDHDLEIKEEKAFLDFDKFLEKLHEYLHEMADTQIRDGLHILGEPPEGSRLD
                     EFLVALTRLANGQVPSLRQSLAEAMGYDYDYLLSNRGRIVSGSKTCGQVIEDLNSLAL
                     QLMAGLHEQGFAIETIPELVEEILGKRNPKIEKVLDYIATTLAPNIDATVDELSAILC
                     ASDGGFVSPGPSGAPTRGMADILPTGRNFYSVDPQAIPSQAAWKVGVAQADALLERYL
                     EDEGCYPESLGMVIWGSPTMRTKGDDIAEVLCLMGVRPVWEERSGRVTGIELIPIEEL
                     QRPRIDVMLRISGFFRDAFPNIVHLVDRAVELVAEQKEPPEQNFLAKHVSADISEKTA
                     AGIDLEQAKTLASYRIFGCRPGAYGAGVSDAIDSKNWKDEKDLAEIYVKWGGYAYGRK
                     NFGATVPDEFRRRLSRLDLTVKNEDTREYDMLDGDDFYSYHGGMIAAVKALKGEIPRS
                     YCGDSSDPDRVKTRSTVEETKHIFRARILNPKWIESMKRHGYKGAGDISRMVDIAFGW
                     DATAEVLEDWMYEELANKYALDRDMQEWLKKVNPHALQNIAERLLEAVERGMWQATEE
                     MKEELRDVYLDIEGWIEDDQPQSNADPGN"
     gene            8791..10515
                     /locus_tag="PLV49_00770"
     CDS             8791..10515
                     /locus_tag="PLV49_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOG97403.1"
                     /translation="MTPNCNKMPCEELKVALVHASISWKEKDKNIAKLLALNREAAQN
                     GARLIVNTELATTGYAFLSRKEIAPLAESIPGPTTEAFGLIANEFGCYICIGLAEHDM
                     RTGIFYNSAALLGPTEGLAARYRKLSPAFRENLWAAKGNLPVPVVQTEFGALAMVICA
                     DSYSYRPARIAALQGARVLLVPANWPPEHHNPERFWRARSLENGIYVLACNRTGMDKT
                     MDCNGAQSFIVNPKGETVAQMSSPDDTIIYGSLPLNGVTTQNALSGRRPQCYGNITLD
                     PYSHISIDFLLGLPQAADFTAATLQSCSEPRNVVENAKRAIRLIDDALAKAAREKGTT
                     INLVVLPELSTSGAIFGAEVAEICSEEIPGKVTDLFSQKAGERDLFIVLGMAERDELG
                     FYNSSVLIGPDGIVGKYRAVHLSWRDRNWASPGDGGFAAYDLPFARIGMLIGHDLLFP
                     EAADSLAKLGADMLCVPALWDDAKSRFIWEARLSEQMHLAVANQWGDCGGLQAIGESL
                     LCSYSRFQEKVIRLSSPPTGDAINIARLGTKDSREKRFLENIDYDVLLDLSNAGKSSN
                     GSQRRPKK"
     gene            10512..>11554
                     /locus_tag="PLV49_00775"
     CDS             10512..>11554
                     /locus_tag="PLV49_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HOG97404.1"
                     /translation="MKIAQFYDGIELYREEKIVYARLMTPHRVLSTCRSSAGGMHDDL
                     MYLYNHQSCEPAGGHMNARMYRLAVDSPEDYKREVADRHNLPFQKCATLGTAANMNNA
                     AICIERFCDLEVVTVCTGGVEGNAGRAGDPATYYEPQDDSAKGQIDLECERRPGTINA
                     MIFVNRELTPGAMVTAVITATEAKTAALQELVVGSKYSDGLATGTGTDQIAVASKLGG
                     KAISFAGKHSKVGELIGRTMHDAVLETLALQNGLTPAGQCSSLAHLERLGTDSREMCQ
                     GIGEFLSRDNADLFEKSFSNIIKDPLTVAAVAALVHLRDKFVWGTLPKSCMPEVMALY
                     GAQISAAVSGKSHR"
BASE COUNT         2662 a         3103 c         2952 g         2837 t
ORIGIN      
        1 taagaagtaa ttgacgaaat tatataagat aaatttattc attaaaattt aaaaattaaa
       61 aaatattaga tattatttac cccgattatt gccagctttc cgaaggagga tagaccatca
      121 ccagattatt gtctattcct aagaatttat tgtaccattg tctgtaaatc gattccggat
      181 cgacatcatt gaaccgatct ggatggagag ctttggctaa gaaaaggcaa aagatgctgt
      241 tatgaactga cctcgttccg ccgttgagga agtataagcg cccatccttg actgcattga
      301 gatcctgcca accggtgcga ttcttaaggc tgtttagtac ctcaatcaat ggctctgcgt
      361 tatttgtcgc cgcaaatccg tccggagtct tgattaccac atgcggatct ctctggatta
      421 tggcttcagg gtccacagtg ggataaggtt ctgcgatgtc atcgaagatc gatcgaccgc
      481 cgcatagaac tatcgcgtca taggtattgt cgccagggcc tcgggttgca aaatccttgc
      541 tgctttcgaa atagagatat gtcctctctt cgtcggtcag gtttttcgtt ctctcctgga
      601 tgaggttcat attggactct tcaaagtcga taagtgaatc agctctctcc tgcctgccga
      661 gcagatatcc cattattctg atggcattat cgaagttgcc gggcttgtgg aaatccatgg
      721 ctataaggcc aattcccctg gagttcagtg ctttctccat tttttcatag gaaccttgtt
      781 tgtaaaagtt ttcgtaacag tgcacgatct ctggattcaa tccgaggatt gcctcggaat
      841 caggctcatt gctgctgccc accgtcggaa catcctttag catatcctgc agctggacgg
      901 catggctctt cgattcgctg atagccagcg gcttgatgcc cagggcaagc agcatctcag
      961 gaccatagct tccggctata gtaacgatat tctctattgg cacgctaacg gttacatttc
     1021 ttccaacgcc gtcgacgatc gtgaggtttg tttcattgcc catgatgatc gattggatct
     1081 gagtgatatc tttttcatcg atccgtccgt cgtagttggc atctgcaaat gccgtggcct
     1141 tttgcgtgcc gccgatgatc tcccgaataa aagcgatgtc cgattcatca attgtatcat
     1201 ccatattggc gtttccgaag atatctagag cgaagttgta tgctattcct gattgaatcg
     1261 aaaacaaaac gcatagcgcc atagtcatca tgaggcgcat attagtggtg gtcctcatac
     1321 atcgtctcct tgccattgaa tccaatacct ccccaacttt gactctccgt cttttatcaa
     1381 tattttttgc cttaaagcaa atttatttga gttaaatagc tttcggtggt ctgataagat
     1441 cattgaattg aaattttaaa aaaaattagc aatcaaccca caatcgaacg cgccagcatc
     1501 ctgtatctct ccgccaggct cgtatcggga gcgccttcga tgactgtggc gttcctctct
     1561 tcgcaggcct gaatggtggg atctcgggga atttcggcta cgacctttgt gtccatctca
     1621 gcagccgccc tccgcacgat ctccaactca tcgacaattc cccgcgcatt caggattatt
     1681 ccttttagct gagcatagcc tcgccctttg aattcgccca ccgcctgagc aatgtttctg
     1741 gcggcaaaaa gggacatctt ctcaccggac gtgacgatgt aaacctcgtc ggcatagccc
     1801 ctgcgaagcg gcatggcgaa acccccgcag accacatcgc ccaggacatc gtaaaggact
     1861 atatcgggtc ggtaggtctc gtaggccttc aatgcctcca gcttctcaaa ggaggcaatg
     1921 ataccccgtc cggcgcagcc cactcccggc gtcggccctc cacattcggc gcataaaact
     1981 ccggcaaaac cgggatagac catgtcctcc agagataccg cagtggcctt ggtcctcaat
     2041 gtgtcaagga tggtaggaac gcttttcccg ttcattagga gacaggtgga gtccgccttc
     2101 gggtcgcagc cgatctgcat cacccttctg ccttgctttg ccagcgctgc agagagattg
     2161 gcagcagtgg ttgattttcc aattccgccc ttgccgtaga tagcgatttt tttcaatttc
     2221 ggaacacctc aggattttga ttaagcagat tgacgatcgt ttgtgtaaga taagcatggc
     2281 cgcgcaggcc gacaaacggg gtcccatcat agcggtaaat gtgatcgaaa gccgggaacg
     2341 cggcatgaat ccttattggt acattcctgg atatctgcag gtcgtaggaa ttgccaaaga
     2401 caatatgcgg attcatgctg gagatagcat cttgcagggt ccagctatcc ggctcgatca
     2461 gaagcctcac atccttcccc aggccaaaat tcgcttcccg gttcaatctc tgcggcccga
     2521 taggggatgc aggcaggaca attaaagatg gattgatgcc ccactcgtcc cgcatcattg
     2581 cagcaaggcc agacgaataa tcgaagggac atgatatcgc cgctctcaag tgatcgatat
     2641 tttgcagaaa attgaccagc tccagtatgg aatatccgta tctttccagt tcagccctca
     2701 ccaatcgttt atccaggcca attcccagaa tgcctgctat atccttcatc cagtcaatgg
     2761 aacctttgac tccgaaggga aggccgtagt agtagggagt gccgaagcgc tctttcagta
     2821 ttgctgcggc ctcaagccct atgtctcttg tcacaaggtt caaagaggcg catggcaggt
     2881 ttttgattgc ctccacatcg gtattgcatg tgaatacggt gttgacccga actcccatga
     2941 ggcccagcat cctttccagc tctgccaggt cggatatgat gttgaaggaa tcgatagtcg
     3001 ggccgattat attaactgtg cagggatttg cccatgagga tgcatcattt gaccttgctt
     3061 cttttgtgca atcctctgca atatttctgg ccagagagcc aaagacctct cttattcctg
     3121 tggtgtagtc tccggcaaaa ccgccgctgg gaaatgatag cattcgagca ctggtggttg
     3181 gttgaatctc ctcgatgatc cccttcagat ccaggccgat catggatgtg acccccgttg
     3241 ctaccagggc gattattctg ggatggtatt tgcgatccag ctcaacgatt gcttctctga
     3301 tcttttcatc cctgcccatg accacgtcgt cctcgtccat tccgctggag aagagcttgc
     3361 ctttgggtga ctggcggttc atgatcatgt agttgaaggc gttgaagttg caggtgccgg
     3421 tggagccgtg ttcaagtatc aggaggtctt tgatgccaga aagcgcccaa agaactccga
     3481 aatagtcgga aggaaccgga aatccattat gcttcaggtc cataaatcag cactctccgc
     3541 cggccagtat cttctctttg tataacaaag cctcaatgcc cgggatccct tccatcagca
     3601 gccgcataac ctcaagcgtg ctttcaaagc ccaaaagata attggaggtt gaaaggatta
     3661 caggccgaat tcccattctg gccaatgaag agcgctcaaa ggcgccgact gagatgtccg
     3721 gagagagttc tgccagaagc tcttcatttt gcagggaatt gtctccccga aaaacgatcg
     3781 gatcgactcc tgcatttaaa agagattgga tgtcgggccg gtcttcaggc tgaatcgttt
     3841 gcatcagcat tgcttttggc tcgatgccga tgctcgttaa gagcagcgct aggtcaaagg
     3901 gcctgccgga gatgccggag actgcacagg tcatgccggc gagagcagca cgggcttttt
     3961 cccgcaaagc ttctgtcttt ttcttcaagt ccgcgacctc gaccgacaga tcgatattta
     4021 gagcagatgc aatattttga taccactctt ctatcgagtc tggatgatag ggttttcggc
     4081 agtaaatata attcgtgcca aacttttctt tcatcatcct ggccaatgga aggccatact
     4141 ggtccaaaac gatattgagc tggaccccag gagcctcttc gagctcagag ggcgtgcagt
     4201 aggatgggat gacgttcttg atccggaccc ctttgccgct tagcaatctt acaagctccg
     4261 tctttctggc agtagaggtc cacaggccca ggatgtttgc ggttctttcc tcctgcggct
     4321 gttctttcat cagctcaaag agggacttga acatgttctc aactcccggt gctgctccct
     4381 cgcaggtgaa attttcagta tgaattgtca gcagcttggc cttgacagag ggccggatct
     4441 catcgatgca agcgtcaaag tcctcgccca cgatctcctg cagacaggag gtgatcagga
     4501 acaaaacctc tgggtgatac tcctggtcgg cctgaaagat ggccactctc accttctcct
     4561 gaaagccaaa tatgatatca tcttgagaat aatttacaaa tagaagatta ttggggcgtg
     4621 ggtcagaggt gagcgacctc atgttcatag agagcttggc attgtaaaga catattgccg
     4681 ggccaaacag cagcgcatag gaattcttga tcagaggcac agttcctgcc accgccctaa
     4741 aagagcagtg ctggccggga tactttgcat gcgcagttgc cgccagagat ccgtcatcag
     4801 gaagctcgct taatctaagg catttgttct cgatactcat ttattttcac agcccaaaca
     4861 atcatttcag gtcaggtcct caatttcctg gatcagcatt gctctgtggt tgatcatcct
     4921 cgatccaccc ctcgatatcc agatagacgt cccgaagctc ctccttcatc tcctcagttg
     4981 cctgccacat ccccctctcc actgcctcga gcagcctctc ggcgatgttc tgcagagcat
     5041 gggggttaac cttcttcagc cactcctgca tatccctatc cagagcatac ttgttcgcca
     5101 gctcctcgta catccagtcc tcaagaacct cggcagtggc atcccagccg aaggcgatgt
     5161 ccaccatcct ggatatgtcg ccagcgccct tgtaaccatg acgcttcatg ctctcgatcc
     5221 atttggggtt gaggatgcgg gcccggaaga tatgcttggt ctcctcaaca gtgctcctgg
     5281 tcttcacccg atccgggtca gagctgtctc cgcagtacga acggggaatc tcgcctttca
     5341 gggccttgac ggctgcgatc atcccgccat gataggagta gaaatcatcg ccgtccagca
     5401 tgtcatactc cctggtgtcc tcgttcttga cagtcaggtc gagacggctg aggcgccttc
     5461 gaaactcgtc gggaaccgtc gctccgaagt ttttgcgacc gtaggcataa ccgccccatt
     5521 ttacgtagat ctcggccagg tctttctcat ccttccagtt cttggagtcg atggcatctg
     5581 agacgcctgc accgtaggct ccggggcggc agccgaatat gcggtagctg gccagagtct
     5641 tggcctgctc caagtcgatt ccagcggccg tcttctcgga aatgtctgca gatacatgct
     5701 ttgccaggaa gttctgctcc ggcggctcct tctgctctgc caccagctct actgctctat
     5761 caacaagatg cacgatattt ggaaatgcat ccctgaaaaa tccgctgatg cgcagcataa
     5821 cgtctatccg aggacgctgc agctcctcta tgggaatgag ctcaatacct gttactctgc
     5881 cgctcctctc ctcccagacc ggccgaacgc ccatgaggca gaggacctcg gcaatgtcgt
     5941 ctcccttggt ccgcatggtg ggactgcccc agataaccat tcccagggat tctggatagc
     6001 agccctcgtc ctccagatag cgctcaagca gggcgtctgc ctgggcgacg cccaccttcc
     6061 aggccgcctg agatggtatg gcctggggat cgactgagta gaaatttctt cccgtgggca
     6121 atatgtccgc cattccccgg gtgggagccc cggagggtcc gggcgagacg aatcctccat
     6181 ctgaggcgca aaggatggct gaaagctcgt ccactgtcgc atctatgttg ggcgcgagcg
     6241 tcgtggctat gtaatccagc accttttcaa tcttggggtt tctctttccc aggatctctt
     6301 ccaccagttc tggaatcgtc tcaatggcaa agccctgctc atggagacct gccatcagct
     6361 gaagggcgag agaattcaaa tcctcaatca cctggccgca ggtcttcgac ccggaaacga
     6421 tccgcccccg gtttgacaaa agataatcgt aatcgtagcc catggcctct gccagagact
     6481 gccggagaga gggtacctga ccgttggcaa gcctggtgag cgcaaccagg aactcgtcca
     6541 ggcgcgagcc ctcgggcggc tcgccaagga tatggaggcc gtccctgatc tgagtgtccg
     6601 ccatctcgtg cagatattcg tgcagcttct ccaggaattt atcgaaatcc aagaacgctt
     6661 tttcctcttt aatctccagg tcgtggtcca gctttgcctc gcagaccttc tcccagatca
     6721 tcttcttctg tgtgggcagt ttggatgggt cctcggaggc ggcctggtag taatcggcga
     6781 gcatcacctc cagctccgcc atctcatcat aagcatcggc attatgcatg acaggaacca
     6841 gatggtcgat gatgcagcag tagctcctcc tcttggcttg agtcccctcg ccggggttgt
     6901 ttatgatata gggatagatg ttgggcagat ctgatattgc gatgtctggg aagcatgaat
     6961 ccgaaagccc aaccgacttt cccggaagcc attccaagga cccatgcttt ccgatgtgca
     7021 tcacgagatc ggcccggaag acatctcgaa tccagcgata gtaggcataa tagtggtgag
     7081 gaatgggatg atcggggctg tggtagatgg cggccgggtc ctccagaaag ccgcgcgggg
     7141 gctgaaggcc gatgaagata ttgccgttga tgacccccgg aattatcagg tttttcttat
     7201 gaacgaaaag ctttcccggc gcctctcccc aggcggaggt catcttctcc tgtacggcca
     7261 cgggaagctg ctcgaaccag gctctgtaat catcctcagt aacggagtct attgccctct
     7321 tcgccagctc ttccggactc ctccagcgcc ggtcgagggt aagcctgctc ttcacatcct
     7381 cgatcagggc ctggccattc tcgggcattc tctcgatcgt atatcccgca tcatccatgg
     7441 ccttcatgat gttcaagacc gatacgggcg agtccaggcc gaatgctgtg cctatgcggt
     7501 cgtcccgggg agggtagttg tggaagatga ttgccacctt cttttgggaa ttgggaatat
     7561 gccgaagctt tgcccagtta aggctcaggc gtacgatctt gttcgtgcgc tcgggaagcg
     7621 gctcgaaccg gatgaccctc gccccggtca gaggatctat gtccgttctc tccctggccg
     7681 ctacagggac ggtgatcagc attccgtcga actcaggcat ggcgattccc atggagatgt
     7741 ccatggggct gcagccctga agcgactcat tccagccagc aagagtgttg taggtcacaa
     7801 tggccttaag cacaggcaca ttcagcctct ttatgaacca ctcctcagat ctggcgacct
     7861 cctgcccctc gccggtgtcc gatccttccc agggcttgat ggagagcgag aacatgaggg
     7921 tgctgatcaa gacgtcaatt atgggccggc cgtccttcat gaagaggttt tccaccaccc
     7981 actctgctcc attcgtcccc cgctcgacgt cctttgcggc atgaaggaag acgggaatga
     8041 catttgcgcc ttgcccctcg atctcctcaa tcagcctgtc gatgaagacc gtatttcccg
     8101 cctgccagag gctctggtaa aaccacaggc ccacagtcgg cctgccggca acgcatttgc
     8161 tctgcagata ctcctgcagc gttggtacgt ggtcgaaatc cggatgataa atgccttccc
     8221 agatgggcct tgcgggctcg gagaattcat aattcgatcc tatgaaatag ttggccaggt
     8281 agaggaggag gttgtagaag ttctcccgtc caccataatc aagatactga gacactttcc
     8341 tgtagacggc atcgtctact gttgacagct tcattagctc aatctcaggc tcatttgaag
     8401 cagactgcgc atagatggag acactgctat cttgaagcct ttggaccaat tcgtcaaagg
     8461 aggaaaggct cttcttcccc ccgtgcagat tgaagatggc tatatggcag cttctggcaa
     8521 aatgctcgaa ctgctcccgt tgggcggcgt catttaggtc ttccccggat ctgactgcca
     8581 cctccgctat ctctccatac tggcgtatca gctcttttaa ggcagatatg agagggaaga
     8641 cgtcgcttgc ttcctgcgtg gttatatagg caattttgac cttcttcgta ataatccatc
     8701 cataacgaaa cattttttta gttagagcta gttgaattga ttaaataaag ctttcgagag
     8761 acatcaaata attcagcgag aatgaatcaa atgactccaa attgcaataa aatgccctgt
     8821 gaagagctga aagttgccct cgttcatgca tcaatttcct ggaaggagaa agataaaaat
     8881 attgccaaac tattggcttt gaacagagag gcggctcaaa atggcgcccg tctcatcgtg
     8941 aataccgagc ttgccactac cggctatgcc tttctaagca gaaaggagat tgctcctctc
     9001 gcagagagca ttcccgggcc cactactgag gccttcggcc taattgcgaa tgagtttggc
     9061 tgctatatct gcattggcct ggctgagcac gacatgagaa cgggcatatt ctataattca
     9121 gcggccttgc tggggccaac cgaaggttta gcggccagat accgcaaatt atctcccgct
     9181 tttcgggaga atctatgggc cgctaagggc aacctcccag ttcccgtggt ccagaccgaa
     9241 tttggcgcgc tcgctatggt gatctgcgcc gactcatact cctatagacc ggcgaggatt
     9301 gctgccctgc agggagctag ggtcttgctg gttccggcta actggcctcc tgagcaccac
     9361 aatcctgaaa gattctggcg ggccagatcc ctggaaaacg gcatttacgt cctggcatgc
     9421 aaccgaacgg gaatggacaa gacgatggac tgtaatggtg cacaatcctt catagtaaat
     9481 cctaaaggag agacggtagc tcagatgagc tctcctgatg ataccattat atacggctct
     9541 ttgccgctaa atggcgtcac gacgcaaaat gctttgagcg ggagaaggcc tcagtgctat
     9601 ggaaacataa ccctggatcc atactctcac atcagcatcg atttccttct cggtttgccg
     9661 caagccgccg attttacagc cgcgacactg cagtcctgct ctgagccccg gaatgtagta
     9721 gaaaatgcaa aaagggcgat taggctcata gatgatgccc tggccaaggc tgccagggag
     9781 aagggaacga cgatcaacct ggtcgtcctg cctgagctct ccacctcggg ggcgatcttc
     9841 ggtgcagagg tggcagagat ctgctcggag gagataccag gcaaagtcac cgatctattt
     9901 tcccaaaagg caggtgaaag agatctcttc atcgttctgg gcatggctga gagggatgaa
     9961 ttgggattct acaacagctc cgtcctgatc ggtcccgatg ggattgtggg caagtacagg
    10021 gcggtccatc tctcttggcg cgataggaac tgggcctccc ccggcgacgg cggttttgca
    10081 gcatatgatc tcccctttgc caggatagga atgctcatag gccatgacct cctgtttccg
    10141 gaggctgcag actcgctagc aaagcttggc gctgacatgc tctgtgtgcc cgccctctgg
    10201 gacgatgcta aatccaggtt catatgggag gcgaggctga gcgagcagat gcatctggcc
    10261 gtcgccaatc aatggggaga ctgcggcggg ctccaggcca ttggagagag ccttttatgc
    10321 agctactccc gatttcagga aaaggtcatc cgtctctctt ctccgccgac tggggatgcc
    10381 atcaatatcg caaggctcgg aacgaaggac tccagagaga agaggttcct ggagaacata
    10441 gactacgacg tgcttttgga tttatcaaat gctggaaaat ccagtaatgg ttcacaaagg
    10501 aggccaaaaa aatgaagatt gcacagttct atgatggaat tgagctatat cgagaggaga
    10561 agatagttta tgccaggctc atgactcctc accgggtcct gtccacctgt cgcagttccg
    10621 ctggcgggat gcatgacgac ctcatgtatc tttacaacca ccagtcctgt gagccggcag
    10681 gagggcacat gaacgccagg atgtaccggc tggcggttga cagtccggag gattacaaga
    10741 gagaagtggc cgaccgccac aacctgcctt tccaaaagtg tgcgacgctt ggaaccgcag
    10801 ctaacatgaa caacgccgcc atctgcatcg agaggttttg cgatcttgag gtcgtgacag
    10861 tatgcaccgg aggggtagag ggaaatgcag gcagggcagg ggatcccgcc acctattacg
    10921 agccgcagga tgatagcgca aaaggacaaa tagatctgga atgcgaaaga agaccgggaa
    10981 caataaacgc catgatcttc gtcaaccgag agctgacacc gggcgccatg gtcaccgcag
    11041 tcatcaccgc cactgaggcc aagactgctg cacttcagga gctagtggtt ggatccaaat
    11101 attcagatgg tcttgctacg ggcactggaa cagaccagat tgcagtggca tcaaaacttg
    11161 ggggaaaagc aatatcgttt gccggcaagc actcgaaggt gggcgagctc atcggtcgca
    11221 ccatgcacga cgccgtctta gaaacgctcg cccttcaaaa cggcctgact cccgcaggcc
    11281 agtgctcctc actggcccat ctggagaggc tgggaactga cagcagagag atgtgccagg
    11341 gaattggaga gtttctgtcc agggacaacg ctgatctctt cgagaagagc ttctcgaata
    11401 taatcaagga tcctctcacg gtagcggctg tggcggctct cgtccatcta cgggacaagt
    11461 tcgtatgggg cactcttccc aaaagctgca tgccagaggt catggccctt tacggcgcac
    11521 agatttctgc ggccgtttcg ggaaaaagcc accg
//
LOCUS       DAOSGP010000012        10830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5117_length_10830_cov_18.272204, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000012 DAOSGP010000000
VERSION     DAOSGP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5117_length_10830_cov_18.272204"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLV49_00780"
     CDS             <1..254
                     /locus_tag="PLV49_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF61 family protein"
                     /protein_id="HOG97405.1"
                     /translation="PRFLWSRLRLPMLIEMSPDFGSGGARIQGEAEVEMVSKILGKER
                     PLNKQIVIYLPDVRELRRKLPTTTQYAFITNLRDRNISD"
     gene            293..844
                     /locus_tag="PLV49_00785"
     CDS             293..844
                     /locus_tag="PLV49_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97406.1"
                     /translation="MAEDKLAEGARQFREKMNAGAYKEAAKIKSDLGLPNSMLQDAVK
                     SAYDANMKKGDYSLAAELAKQYDLPSDHRLEAAQRSFYRKIDSEFYRAAAEYAKEFGL
                     PEDMVRQAAIQAFNKSMSMGMVKNAAEIADDFDLPRPMKQEAAKKSFEQHMQAGLYRK
                     ALKIAQKYDLPEEMVAEAEKKIS"
     gene            905..2254
                     /gene="thiD"
                     /locus_tag="PLV49_00790"
     CDS             905..2254
                     /gene="thiD"
                     /locus_tag="PLV49_00790"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOG97407.1"
                     /translation="MSKVVLTIGGSDSGGGAGIQADIKTFSVLGLHGTSALTAITAQN
                     TQGVQHVYALSPEEVRAQLESITEDFSIAWAKTGMLHSAQIVDTVADHLQANDIPLVL
                     DPVIEAEAGGRLLRPDAVSALKERLIPLAGVVTPNIFEAEALSGIRVRDMDSAAHAAR
                     GIMSLGAQAVIVKGGHLDCTDLLMVRNKSTLLPSERISGENHGIGCTFSAALTSFLAL
                     GCSIENAARNAKEFAKSALRGSLRVGKGVGPVNQSAALRVEASRYRVLCDLEVAVDLL
                     ENSHELFDLMLKGGAGPAMAIPGARCPEDVAALVGGLTKIEERVCRFGGFRFGGRSDS
                     ASVILAAMKIDPLAKAAIDLEPNALLSCRELGLPIIDRARLDELAAIGQCHLKGEEEL
                     SEGEGSALLPAAIWDEGVLGKEPRLYLLGASASLLVELLASLPASRAWGILEDDSKI"
     gene            2304..2519
                     /locus_tag="PLV49_00795"
     CDS             2304..2519
                     /locus_tag="PLV49_00795"
                     /inference="COORDINATES: protein motif:HMM:NF002242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HOG97408.1"
                     /translation="MGSVKPSYIKNFAMDLLRTYESSFSPDFEQNKLRVSEYTDIKNK
                     TIRNRVAGYISNVMRQRSTRREVEVDV"
     gene            2512..3387
                     /gene="dapA"
                     /locus_tag="PLV49_00800"
     CDS             2512..3387
                     /gene="dapA"
                     /locus_tag="PLV49_00800"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOG97409.1"
                     /translation="MYSGVLPAIITPFHEDKSLDEEGLKRNVEYLSKTGIAGMVPCGT
                     TGEAATLTFEEHKRVIEIAVENSTVPVIAGTGSNNTREAVELTCHAAEAGADAALLIT
                     PYYNKPNDRGMFEHFKTVAEKCDIPIVLYNVPKRTGIDLKPELVAKLSRIKNIVAIKE
                     ASGSLSQLSQIIEQTRGSDFSVLCGDDDLTLPAMALGAKGVISVVANVAPRKTVAMVD
                     AMMKGDLEKARSLHYELAPLVRAMFLETNPIPVKTAHKYLGLAGGPLRLPLGEMAADK
                     EKMLKELLVTLGERA"
     gene            3384..4169
                     /gene="dapB"
                     /locus_tag="PLV49_00805"
     CDS             3384..4169
                     /gene="dapB"
                     /locus_tag="PLV49_00805"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOG97410.1"
                     /translation="MTRIAITGALGRMGTLVIQEAARIEGMELVAGLDAVGAGKPLQG
                     GVLVKDASQLEQVLKETRPDVLIDFTIAKAAVKNVCIAASLGVDLVVGTTGISSEQHS
                     EMREAIEGNVAAVITPNFSVGVNLFWKLVADAAAALKGYDFDIEIIEAHHNQKKDAPS
                     GTALATLEAIRRARGESDLSEVVYGRKGIMPRGKEIGVHAVRAGDIVGDHTVLLAGPG
                     ERLEIRHQAHSRAAFASGAVRAAAWVVGRKPGIYGMADVLESR"
     gene            4166..4591
                     /locus_tag="PLV49_00810"
     CDS             4166..4591
                     /locus_tag="PLV49_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97411.1"
                     /translation="MNQLTEKGMFLLFNGERHNPLSQSRNVIGFCSTCGSDLESLAYY
                     STASEWLVSAQCAKGHLALIRYGRDWSWLEDLPLEFLKEEVKVADLPREKLDAIFTPA
                     EIRDMIACQEGSPYVRQNIYRARTKYERFEKLFGIKIDI"
     gene            complement(4598..5437)
                     /locus_tag="PLV49_00815"
     CDS             complement(4598..5437)
                     /locus_tag="PLV49_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG97412.1"
                     /translation="MFIQLEEINSRPEPFGFYTAADLWTDEHTSEQMLAYHLNADIDV
                     SSRRGEFIDRSVEWIASRFSIGADTRIADFGCGPGLYANRLAKVGASVIGIDFSKCSI
                     KHAREVAREVGLTIEYINQNYLDFETEHRFDLILMIMCDFCALSPSQRRIMLAKFHSM
                     LLPGGSVLLDVYSQSAFKQRKEASSYGVNLLDGFWSAEKYYGFLNTFKYDDAMVVLDK
                     YTIVESARKRTVYNWLQYFTPESLWREFEVAGFAEQELYADVAGAPFDKMASEFAIIA
                     QKK"
     assembly_gap    5870..5969
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5970..6497
                     /locus_tag="PLV49_00820"
     CDS             <5970..6497
                     /locus_tag="PLV49_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97413.1"
                     /translation="QQNMRNAMRLSMDLELQDVPGQLLLAMQPLSDNKANIISVVHHR
                     DRKTPRGMIPVRFVVEMDRSRIEAVQARLQESGISVVRAGEDRFIEAVSVVLVGHVLD
                     SDLGDTINRIDSTGYAEVMDLSLTMPGVNEHSSAYLKIRATGKAEIQKALTILRNVGE
                     EKKLLVIEPIETEAV"
     gene            6494..7501
                     /locus_tag="PLV49_00825"
     CDS             6494..7501
                     /locus_tag="PLV49_00825"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOG97414.1"
                     /translation="MRDIKVSLVGYGIVGHGVVDVLTRKREILRDMGLNLMLVSVTDH
                     TGTVLSEDGVDANKILGERTLANVATSSMKGKEAINAIDSDLMIEVTPTNIVHGQPGL
                     GHMEAALASGKHVVTSNKGPLVVSYGSLKKLAEDNGVMLKFEATVGGTMPLISLVERT
                     LVGNTILGVRGIFNGTCNYILTRMTEEGIPYAHALSEAQDMGIAEADPSYDVEGVDTA
                     GKVLILANSLFNMNVKYSDVNVTGITGVTPEALALAKKRGYAIKLIGDVPALTVRPTL
                     VPLDSPLAVAGTLNSAAIYTDLSGTITVSGLGAGSIETASAILTDIISIYRTKRIDAI
                     F"
     gene            7523..9193
                     /locus_tag="PLV49_00830"
     CDS             7523..9193
                     /locus_tag="PLV49_00830"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOG97415.1"
                     /translation="MSSFLSFAELCQRVEGVSGSLEKIDLVAAFLKNLDDDELAVASG
                     FIMGDLFSPSLDLVMGVGPSILYEAMARACGCSTERISDMLRATGDPGLVASAAVDKK
                     KPIGFAAFIKDEPLSIKEVYQRLVAVARASGKGSQDAKVKNLQYLFSQATPLEAQYIA
                     RLAIEDMRIGIGEGGVRDAIAQAFSQDASKVERAYNLTNDIGLVAVNARKGTLADLNV
                     LINHPIKMMLAQVGEGISASMQELGSAAIEWKYDGARVQIHKDGKRVRIFSRRLEDVT
                     RSLPEIVRLAQEVRADKAILDGEVVAIGKDSRPLAFQEILKRFRRKYNVEKQAKETPL
                     HLFLFDLIYLDGQSTVDLPLTRRRELLEGIADPSILADQVISDSVQKAEEIYHQALEK
                     GHEGLILKNSMSVYAPGKRGKNWLKIKPVMETLDLAVIGAKWGEGRRASFLGSYRLAC
                     LDEATSKLLDIGWVATGLTDEALAELTELFRELILVQNGMEVELKPAVIFEVAYEEIQ
                     KSQSYSSGYALRFPRLVRVRDDKSLEEADSLERVESLYRVQRGRNNSR"
     gene            9220..10506
                     /gene="hisD"
                     /locus_tag="PLV49_00835"
     CDS             9220..10506
                     /gene="hisD"
                     /locus_tag="PLV49_00835"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOG97416.1"
                     /translation="MITKRLSELEDEDLRSLLSRDVGIQDVLPAVNEIIMEVGSKGDE
                     ALFKYTEKFEGAHLDDLRVTQEEIDLSYDLVEDRLIEALSSAADHIFYFHNEQKEHDL
                     WMSEASPGVLVGQKVVPLDSVGAYVPGGRAAYPSSALMTVIPAKVADVPRIVVCTPPQ
                     KDGSINPLTLAAADMAGADEIYKLGGAQAIAAMALGTDSIERVDKIVGPGNVYVTAAK
                     MLTRGSVEIDFPAGPSEVLILADRTADPGVIAADMIAQGEHDPKSISVFVSLDDLLAS
                     AVAEELNIQAPGAARSDIVSESLEHSAILLADDMDEALDFVNAFAPEHLEIITKEPMN
                     VLRSIRNAGSVFLGKFTPVAAGDYASGTNHVLPTSGYARIFSGLNVDHFTKKMTLQMI
                     TDEGLLGLEETITTLAEAEGLLAHAESVRRRLEPRE"
     gene            10538..>10830
                     /locus_tag="PLV49_00840"
     CDS             10538..>10830
                     /locus_tag="PLV49_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HOG97417.1"
                     /translation="MSEHLLEMAGARVRIRDGKVEVLTEPAIRSCPLRQDLYGIKVES
                     KETVKRVLEEHIAELGMYGPKRVLELEDKPVSFGASEILSDALTEGLIDAAVLV"
BASE COUNT         2626 a         2667 c         3185 g         2252 t
ORIGIN      
        1 ttcccagatt cctgtggagc cgcctcagac tgccaatgct cattgagatg tccccggatt
       61 ttggcagtgg tggtgcccgt attcagggtg aggcagaggt ggagatggta agcaagatac
      121 tgggcaaaga gaggccgctg aacaagcaga tcgtaatcta ccttcccgat gtgcgagagc
      181 ttcgccggaa gcttcctaca accacacagt atgcattcat taccaatctc cgggacagaa
      241 atatcagcga ctgaaggatc gatcaaatca aaagatagcg gtggaggatt atatggcaga
      301 agataaactg gcagaaggcg caagacagtt tagagagaag atgaacgcag gggcctacaa
      361 agaggcagca aagatcaagt cggatctggg ccttccgaac tcgatgcttc aggatgcggt
      421 taagtcggca tacgatgcca atatgaagaa gggggattat tctctggctg ccgaactggc
      481 caaacaatat gatcttccca gcgaccatcg tctggaagcc gctcagaggt cgttttatag
      541 aaagatagac agcgagttct accgggcagc ggcggagtac gccaaggagt tcgggctgcc
      601 agaggatatg gtccggcagg cggccatcca ggctttcaat aagagcatga gcatgggcat
      661 ggtcaagaac gctgctgaga tcgccgatga cttcgacctc cccagaccaa tgaaacagga
      721 ggcagcaaag aagtcctttg agcagcatat gcaagccggc ctctaccgca aagccctgaa
      781 gatcgcccag aagtacgacc ttccggaaga gatggtggca gaggcagaga agaagatatc
      841 ctgaaagata tacccaagcc agataaatct gcttgaagcc gtcctgattt tcaattccca
      901 agatatgtcg aaagtagtgc tgactatagg cggatcggat tcagggggag gcgccggaat
      961 ccaggcggac atcaagacat tttctgtcct gggcctgcat gggacatccg ccctgaccgc
     1021 catcaccgcc cagaacaccc agggagtgca gcacgtctat gcactctctc ccgaagaggt
     1081 aagagcgcag ctcgaatcga tcacagaaga cttttccatt gcctgggcca agaccggcat
     1141 gctccactcg gcccagattg tggatacagt agccgatcac ctccaggcaa atgatattcc
     1201 tttggtcttg gatccggtga tcgaggctga ggctggcggc aggctccttc ggcccgatgc
     1261 ggtatctgcc ctgaaagagc gcttgatccc cctggcaggg gtggtcaccc ccaacatctt
     1321 cgaggcagag gctctttctg gaatccgcgt tcgcgacatg gatagtgctg ctcatgccgc
     1381 ccgtggtatc atgagcctgg gggcgcaagc ggtgatcgtc aagggagggc acctggattg
     1441 caccgatctg ctcatggtgc gcaacaagtc cactcttctg ccctcagaaa ggatatccgg
     1501 agagaaccac ggcattggat gtaccttctc tgccgcttta acctcttttc ttgccctggg
     1561 ctgctcaatt gaaaacgccg cccggaatgc aaaggagttc gcaaagagcg cccttcgcgg
     1621 gagcctcagg gtgggaaagg gagtgggccc ggtaaaccag tctgcagccc tgagagttga
     1681 agcgagccgt taccgggttc tgtgcgatct ggaagtggcg gtggatctgc tggaaaattc
     1741 tcacgagctc ttcgatctca tgctgaaggg aggagcaggg cctgccatgg ccattcccgg
     1801 cgccagatgc cctgaggatg tggcagcgct ggtgggagga ttaacaaaga tcgaggagag
     1861 agtatgccgt tttggaggat ttcgattcgg tggcaggagc gatagcgctt ctgtgatcct
     1921 tgccgcaatg aaaatagacc ctctggcaaa agcggccatc gatttagagc caaatgccct
     1981 cctatcctgc agagagcttg gcctcccgat aatagatcgc gctcgattgg atgaactggc
     2041 agcaattggc caatgccatt tgaagggcga ggaggagctg agcgaagggg aaggctcagc
     2101 tctactgccc gctgcgatat gggatgaggg cgtcctgggt aaagagccca ggctttatct
     2161 gctgggggct tcagcatcct tgctggtcga actgttggcc agcttgccgg cgtccagggc
     2221 ttggggcatt ctagaggatg atagcaagat ataaattgtg gagcaaggat gcacaaagga
     2281 ttgatctagc tggagtgttg attatgggaa gcgtaaagcc tagttatatt aagaatttcg
     2341 cgatggacct gttgagaacc tacgagagct catttagccc tgactttgag cagaacaagc
     2401 tgagggtatc ggagtatacc gatatcaaga acaaaaccat ccgcaaccgc gtagcaggct
     2461 acatatccaa tgttatgagg cagagaagta cgagaagaga ggtcgaggtt gatgtatagc
     2521 ggcgtgcttc ctgcgataat cacgccattt catgaggaca agagcctgga cgaggagggt
     2581 ctgaagagaa acgtagagta cctgagcaaa acaggaatag caggcatggt tccctgcggc
     2641 actaccggtg aggcggcaac tctgaccttc gaagagcata agcgagtgat agagatcgca
     2701 gtggagaact ccacggttcc ggtgatagca ggaactggtt ccaataacac ccgcgaggcg
     2761 gtggagctga cctgtcatgc cgcagaggcg ggcgcagatg ctgccctgct gatcacccca
     2821 tactataata agcccaatga tcgcggcatg ttcgagcact tcaagaccgt agccgagaag
     2881 tgcgacatcc ccatagtgct ctacaatgtt cccaagagga ccggaatcga cctcaagccg
     2941 gagcttgtgg caaagctctc tcgaataaag aatatcgtgg ccataaagga ggccagcgga
     3001 agcctttccc agctatctca aattattgag cagacccggg gaagcgactt ttccgttctc
     3061 tgtggcgatg atgatcttac gcttccagcc atggccctgg gtgcaaaggg cgtgatctca
     3121 gttgtggcca atgttgcccc cagaaagact gtggcaatgg tggacgccat gatgaagggc
     3181 gacctggaga aggcaagatc cctgcactat gagcttgctc ccctggttcg agcgatgttc
     3241 cttgagacca accccatacc tgtgaagact gctcataagt acctgggatt ggcaggcggc
     3301 ccattgcgcc ttcccctggg ggagatggct gcagataaag agaagatgct aaaggagctg
     3361 ctggtgaccc tgggcgagag agcatgactc gaattgcgat tactggagcg ctggggcgca
     3421 tgggcaccct ggtcatccag gaggcggcca ggattgaggg gatggagctg gtggcaggcc
     3481 tggatgcggt cggcgcggga aagccgcttc aagggggcgt tctggtaaaa gatgcttcac
     3541 agctggagca ggtcttgaaa gagaccaggc cggatgtgct catcgacttc acaatagcca
     3601 aagcggcagt gaaaaacgtc tgcatagcgg cctctctagg tgtcgatctg gtggtgggaa
     3661 ccacaggcat ctcttcggaa cagcattccg agatgagaga ggcaatagaa ggcaatgtgg
     3721 ccgcggtcat aacccccaac ttcagcgtgg gagtaaacct gttctggaag ctggtggctg
     3781 atgctgcggc tgctctcaag ggctacgatt tcgatataga gatcatagaa gcccatcaca
     3841 atcaaaagaa ggacgcaccc agcggcactg ctctggccac attggaggct ataagaagag
     3901 ctcggggaga gtcggatctc tcggaggtcg tctatggccg gaaggggatt atgccgcgag
     3961 gcaaagagat tggagttcat gccgtgcggg caggtgacat cgtaggagac cataccgtcc
     4021 ttttggcggg tcctggcgag aggctggaga tccggcacca ggcccatagc agagccgctt
     4081 ttgccagcgg tgcagtgcgt gcagccgcct gggtggttgg caggaagcct ggaatttacg
     4141 gcatggcgga cgttctagaa tccagatgaa tcagttgacg gagaaaggga tgttccttct
     4201 cttcaatgga gagaggcata atcccctttc ccaatcaagg aatgtgattg gcttctgcag
     4261 cacctgtggc tcggatctgg aaagcctggc ctattatagc actgcttcag agtggctggt
     4321 ttcggcccaa tgtgcaaaag ggcacctggc actgattcgc tatggcaggg actggagctg
     4381 gctagaggat ctgccgcttg aattcctaaa ggaagaggtg aaggttgctg atctgccccg
     4441 ggagaagctg gatgccatct tcaccccggc cgagatcagg gatatgatcg cctgccaaga
     4501 gggatcgccc tatgtcaggc agaacatcta ccgcgccagg acaaagtacg aaaggttcga
     4561 gaagctcttc ggcattaaga tcgacattta ataatcacta tttcttttgc gcaatgatgg
     4621 caaactcaga ggccatttta tcaaacggcg cgcccgcaac gtcagcatag agctcttgct
     4681 ctgcgaaacc tgccacctca aactcgcgcc acaacgactc gggagtgaag tattgcagcc
     4741 agttgtagac agttcgtttt ctcgccgatt cgacgatggt gtacttatcc aaaaccacca
     4801 ttgcgtcgtc gtacttgaaa gtgttcagaa atccatagta cttctccgcg gaccagaatc
     4861 catcgagaag attcactcca tacgagcttg cctccttgcg ttgcttgaaa gcactttggg
     4921 aataaacatc gagaaggaca gacccacctg gcagaagcat ggagtggaac ttagcgagca
     4981 taatcctgcg ctgcgacggg cttaaagcgc agaagtcgca catgatcatc agaatgagat
     5041 cgaagcggtg ttctgtttcg aaatccaggt agttctggtt tatgtattca atggtcaatc
     5101 ccacctctcg cgcgacttct cgggcatgct tgattgagca cttagagaaa tcaataccaa
     5161 ttacactagc accgaccttc gccagccgat ttgcatagag cccgggacca cagccaaaat
     5221 cggcgattcg ggtgtcggcg ccaatactaa agcgggatgc aatccattcc accgatcgat
     5281 cgatgaactc gcctcgacgg gatgatacat cgatatcggc attgagatga tatgcgagca
     5341 tttgctctga ggtatgttcg tcggtccaca ggtctgccgc tgtgtagaac ccaaatggtt
     5401 caggtcgact attgatttcc tccagctgaa tgaacatatc tctccaggtt gtaaatgaat
     5461 ccacacttcc ggatagtaat gttggtatat aaaaatatcc gtactactcg caatggttta
     5521 cagaacccac taacgtccct tattagacct aattgctata aaataaatag aatagcccct
     5581 tggcccgcct gactatcaaa acaggctggt caagaacacc aggggcacta aaaacaatac
     5641 tacgaaaccg ataagcatca atccaattgc cagaccattt ccacctgcca tgaagatcct
     5701 cctactttcg ttggcaactg cttggatgag gtaatattaa ttactatcgg acagacgccc
     5761 accctcggat cccatctgca caaggtttat ttaatttaac cagcaggaga attccagcaa
     5821 aatatgagga atgctatgcg actttccatg gatttagagc tgcaggatgn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc agcaaaatat gaggaatgct atgcgacttt
     6001 ccatggattt agagctgcag gatgttcccg gccagctgct ccttgcgatg caacccttga
     6061 gtgataacaa ggccaacatc atcagcgtgg ttcaccacag ggaccgcaag actccccggg
     6121 gcatgatccc ggttcgattc gtcgtggaga tggaccgctc aagaatcgag gctgtccagg
     6181 ctaggctcca agaaagcgga atctccgttg tccgggcggg agaggaccga ttcatcgaag
     6241 cagtatcagt tgtgctagtg ggccatgtgc tggatagcga tctgggagac accataaacc
     6301 ggatcgactc caccggctac gctgaggtga tggacctttc cctgaccatg cccggcgtga
     6361 atgagcactc ctctgcctac ctcaagattc gcgccaccgg caaggctgag atccagaagg
     6421 ctttgaccat tctgcgcaat gtgggcgagg agaagaagct gcttgttatc gagcccatag
     6481 agacggaggc agtatgagag atattaaggt ctccttggtt ggctacggca ttgtgggcca
     6541 cggagtggtt gacgttctca ccagaaagag agagatcctc cgggatatgg gcctgaacct
     6601 gatgctggtc agcgtcaccg atcacacggg aacggtattg tccgaggacg gtgtggatgc
     6661 gaacaagatc ctgggcgaga ggaccctggc caatgtggcc acatcgagca tgaaggggaa
     6721 agaggccatc aatgccatag actccgacct gatgatcgag gtcactccca ccaacatcgt
     6781 tcacggccag cctggtctgg gccatatgga agctgcgctc gcctcgggca agcatgtggt
     6841 tacatccaac aaagggccac tagtggtatc ctacggcagc ctgaagaagc tggcagagga
     6901 taacggcgtg atgctgaagt tcgaggccac agtcggcggc accatgcccc tgatcagcct
     6961 ggttgagcgc accctggtag gcaatacgat cctcggcgtc aggggcatat tcaacggcac
     7021 ctgcaactat attctaaccc gcatgactga ggagggcata ccctatgctc acgccttgag
     7081 cgaggctcag gatatgggca ttgccgaggc cgaccccagc tatgatgtag agggggtgga
     7141 taccgctgga aaggtgctaa tcctggccaa ctccctgttc aacatgaatg tcaagtactc
     7201 cgatgtgaat gtgaccggca tcacaggagt cactccagag gctcttgctc tggccaagaa
     7261 gagaggatat gccataaagc tgatcggaga tgtgcccgca ctcacagtca ggcccactct
     7321 ggtgcccctg gacagcccac tggccgtggc cgggactctg aactccgctg ctatttatac
     7381 cgatctatcc gggacgatta ctgtatcggg tcttggtgca ggctccatag agacggcctc
     7441 ggccattctt accgatatca tcagcatcta ccggactaaa cgcatagatg ccatattcta
     7501 gatcaatgac tagggtcaga taatgagcag cttcttgagc ttcgccgagc tctgtcagcg
     7561 agtggagggg gtctccggct ctctggaaaa gatcgaccta gtggcagcat tcctgaagaa
     7621 tttggatgat gatgagctgg cagttgcctc cggattcatc atgggggatc tcttctcccc
     7681 cagcctggac ctggtgatgg gcgtggggcc gagcatcctt tacgaggcga tggcacgcgc
     7741 ctgtggatgc tcgacggaga ggatatcaga tatgctgcgc gccaccgggg acccggggct
     7801 ggtggcatct gcagccgtcg ataagaaaaa gcccatcgga tttgctgcct tcatcaaaga
     7861 tgagccgctc tccatcaaag aggtctatca gaggcttgta gccgtggccc gggcctcggg
     7921 aaagggaagc caggatgcca aggtcaagaa cctgcagtat ctcttcagcc aggccactcc
     7981 tcttgaagcg caatatatcg cccgcctggc gatagaggat atgagaatcg gaataggtga
     8041 aggaggagtt agagatgcca ttgcccaggc tttttcccag gatgcgtcaa aagtggagag
     8101 ggcctacaac ctgaccaacg atatcggcct ggtggcagtc aatgcccgca aagggacgct
     8161 cgcggatctg aatgtgctga tcaaccatcc catcaagatg atgctggccc aggtgggaga
     8221 gggaatttcc gcctccatgc aggagctggg gtctgcggct atagagtgga agtacgatgg
     8281 tgccagggtg cagatccaca aggatgggaa gagggttcgc atcttctccc gccggctgga
     8341 ggatgtgacc aggtccctgc ccgagatcgt ccgtctggcc caggaggtca gggcggataa
     8401 ggccatcctg gacggagagg tggtggccat tggcaaagac tccagacccc tggccttcca
     8461 ggagattttg aagcgctttc gcaggaagta caatgtggaa aagcaggcca aagaaacgcc
     8521 tctgcactta tttttattcg atctgatata tcttgatggc caaagcacag tggatctgcc
     8581 cctgaccaga cggcgggagc tgctggaggg tatcgccgat ccatcgatac tggcagatca
     8641 ggtcatatcc gacagcgtcc agaaggcaga agagatctac caccaggccc tggaaaaagg
     8701 ccacgagggg ctgatcctca agaattcgat gtcggtatac gctccgggaa agagaggcaa
     8761 gaactggctt aagataaagc cggttatgga aaccctggac ctggcagtga taggggcaaa
     8821 atggggcgag ggcaggagag ctagcttcct gggatcatac cggctggcct gcctggatga
     8881 ggctacgagc aagctcctgg atataggctg ggtggccact ggcctaacgg acgaggctct
     8941 ggcggagcta actgagctgt tccgagagct gattctggtg cagaatggga tggaggtcga
     9001 gctgaagccg gcggtgatct tcgaggtggc ctatgaggag atacaaaaga gccagagcta
     9061 ctcatcggga tatgcacttc gttttcccag attggtgagg gtgagggatg acaagtccct
     9121 ggaggaagcg gatagcctgg agagagtgga atccctctac cgggtgcagc gaggaaggaa
     9181 taactcccgc tgaaagggta ttatgaagga ggttttaaag tgataactaa aaggctgagc
     9241 gagctcgagg atgaggatct gcgttcactt ctctccaggg atgtgggcat tcaggatgtt
     9301 cttcctgcag taaatgagat catcatggag gtcgggtcga aaggagatga ggctctattc
     9361 aagtacactg agaagtttga aggggcacac ctcgatgacc tgcgcgtgac ccaggaggag
     9421 atcgatctat catacgacct ggtagaagac aggcttattg aggcgctctc ctcggcagcg
     9481 gatcatatct tttatttcca taatgaacag aaagagcatg acctctggat gagcgaggca
     9541 tctcctgggg tgctggtggg ccagaaggtc gttcctctgg attctgttgg agcttacgtt
     9601 cccgggggca gggcggccta ccccagctcg gccctgatga ccgtcattcc cgccaaggtt
     9661 gctgatgtgc ccaggattgt ggtctgcacc ccgccccaaa aggatggatc gatcaatcca
     9721 ttgacgctgg ctgctgcgga tatggcagga gctgacgaga tctataagct cggaggggct
     9781 caggcgatag cggccatggc tttgggcaca gatagcattg aaagggtgga caagatcgtg
     9841 gggccgggca atgtctatgt cactgccgcc aagatgctta cccgaggctc ggtggagatc
     9901 gattttcctg ctggtcccag cgaggtcttg atcctggccg atcggacagc ggatcctggg
     9961 gtgatagctg ccgatatgat cgctcaggga gagcacgatc ccaaatcgat ctccgttttc
    10021 gtatccctgg atgatctcct ggccagcgcc gtggcggagg aattgaacat tcaggctcct
    10081 ggggctgcac gcagcgatat tgtctctgag agcctggagc acagtgccat tctgctggcg
    10141 gacgatatgg atgaggcgct tgattttgtc aatgcttttg cccctgagca tctggagata
    10201 atcaccaagg agcccatgaa cgttctgagg tccattcgca atgcagggag cgtgttcctc
    10261 ggaaagttca ccccggtggc ggcaggggac tatgccagcg ggacgaatca tgtgcttccc
    10321 acctcgggat atgctcgcat cttctccggc ctgaatgtgg accacttcac caagaagatg
    10381 accctgcaga tgatcaccga tgagggattg ttggggctgg aggagacgat taccactctg
    10441 gccgaggcgg aggggctgct cgcccatgcc gaatcggtga gaagaaggct ggagccgcga
    10501 gaatgaaccc cgcggcctga tatctgtctg accagaagtg tcagagcatc tgctggagat
    10561 ggccggggcc cgggtcagga ttcgggacgg gaaggtcgag gttctaactg agcctgccat
    10621 tcgctcatgt cccttgcgtc aggatctgta cggcatcaag gtcgagagca aggagacggt
    10681 gaagagggta ttagaagagc atatagcaga gcttggcatg tacggcccga agagagtcct
    10741 ggagctggaa gataagccgg tgagctttgg agcttcggag atcctctcag atgccctgac
    10801 agaagggcta atagatgccg cagtgctggt
//
LOCUS       DAOSGP010000013        10555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5305_length_10555_cov_19.452857, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000013 DAOSGP010000000
VERSION     DAOSGP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10555
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5305_length_10555_cov_19.452857"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..647)
                     /gene="fae"
                     /locus_tag="PLV49_00845"
     CDS             complement(141..647)
                     /gene="fae"
                     /locus_tag="PLV49_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HOG97418.1"
                     /translation="MSQSFQRVLVGEALVGEGPEVAHIDLVMGPRDSPVEKAFVRALA
                     SPSRGHTPLLAVLAPNLPVKPSTLMVNKVTIKNAQQAMMVFGPVQAAVARAVMDCVES
                     GVLPEELADDLFIIVSVFVEWDAKDKDKVYEYNYQATKIAIERAANGEPKPVDILAAK
                     STAKHPFA"
     gene            738..1283
                     /locus_tag="PLV49_00850"
     CDS             738..1283
                     /locus_tag="PLV49_00850"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HOG97419.1"
                     /translation="MRVIVINNYGQFNHLIRRSIRDKVDCDLASNQTPPEDIDADGLI
                     LGGGPSLDRAGNCRDYLLKLDIPILGICLGMQIMGTTFGGQVTAGSIGGYAQVDVELI
                     DEDDILKGFPAKFKTWASHADQVSLLPSQFQRLATSRICDIEAMKHRNRPLYGVQWHP
                     EVVHTEHGEELLDNFINICKR"
     gene            1368..1988
                     /locus_tag="PLV49_00855"
     CDS             1368..1988
                     /locus_tag="PLV49_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOG97420.1"
                     /translation="MPDKNLLLADLEESYAAARRLSARSLAGQIERIGFQCLGCGQCC
                     RGDDNSVVVFPFEVRRIMAKRGLDWLEVAQPPEEGEWDNNGNFHTLEWRLKKEGLRCR
                     FYDEGQCTIYGQRPLLCRTYPFYLVEGELRCSECPGLGMRINEDAAQEIAGQLILRHI
                     TEIVEAIALTKKYQDFQRGGCKEGNCCIIHDSEGEHKIPLFTAPRS"
     assembly_gap    2029..2128
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2129..3234)
                     /gene="dnaJ"
                     /locus_tag="PLV49_00860"
     CDS             complement(<2129..3234)
                     /gene="dnaJ"
                     /locus_tag="PLV49_00860"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOG97421.1"
                     /translation="MPDKKDYYDVLGVSKDASEKDIKTAYRKLAMKHHPDRSDDPGAE
                     EMFKELSEAYAVLSDPDKRQKYDQFGHAGINSQYSQEDLFRGVNFEDLLRGFGAGGES
                     IFDMFFGGGGGRRGPARGRDLRYDIDLTLEQASRGLETTIEVPRTENCPKCQGSGAKP
                     GTSPVTCSTCRGSGQVTRAQNTPFGQMVTSSTCPKCGGRGELITSPCEDCSGTGRARK
                     ARKINIKIPAGVEDGQHLKLRGQGDSAGPKGVSGDLYVVINIQPHRLFQRLDSDLIME
                     TPISFTQAALGADLEVPTLNGRARIKVPAGTQTGTVFRLRGKGMPRLHGTGSGDLHVR
                     VKINTPASLTARQRQLLEELAEEFGEAKRSSTEK"
     gene            complement(3308..5176)
                     /gene="dnaK"
                     /locus_tag="PLV49_00865"
     CDS             complement(3308..5176)
                     /gene="dnaK"
                     /locus_tag="PLV49_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOG97422.1"
                     /translation="MSKIIGIDLGTSNSAAAVLIGGRPTIIPSAEGTSIGGKAFPSYV
                     AFTKDGQVLVGEPAKRQAVTNPDGTVQGIKRKMGTDYKVRVFDKEYTPEDISAQVLRK
                     IKTDAETYLADSVDKAVITVPAYFNDNQRQATKDAGTIAGLEVVRIINEPTAAALAFG
                     LDKVGEHKIMVFDLGGGTLDVTIMEIGEGVFEVLSTSGDTQLGGRDMDDRLTNYVLDR
                     FKQESGVDLRGDSMAMQRLREAVEVAKIELSSVLQTNLNLPYITADASGPKHLSMTIA
                     RSKLEELVADVLERCRGPMIQALKDSKLEKSDIGKIILVGGPTRMPMVQEFVKNFLGK
                     DIERGVDPMECVAMGAAIQAGVLGGEVKDLLLLDVTPLSLGIETLGSVTTKLIERNTT
                     IPTRKSQIFTTAADNQTSVEVNVLQGERPMAQDNVSLGRFTLVGIPPAPRGIPQIEVT
                     FDIDANGIIHVSAKDLATKKEQRITITAPHKLSQQDIDAKIKDAERFAADDLKRKEEI
                     EVKNAAESLIYASEKMLKEAGDIATSEQKEKIEKAISDLKNAQTGGEISEIKAKTESL
                     QNAIYELSAAMYQKAAQDQQAQQGQAGQGSGPEAGQDSTGQDQTVDADYEVVNDKK"
     gene            complement(5265..5786)
                     /locus_tag="PLV49_00870"
     CDS             complement(5265..5786)
                     /locus_tag="PLV49_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOG97423.1"
                     /translation="MDDDTAVSAQDEEEDESSLTARIDELEGKLVEMQILSEERLDQL
                     MRCRADLDNLMKRSVREKEDTVRYASEKLVQKLLPVLDSLEQAAKHDEGQKVLHMQLL
                     GVLFTEGLVPIEAVGKKFDPYRHEALFQVKKDDLEEDIVAEEIQKGYLFNSRVIRFSK
                     VAVNKPLKAEGCK"
     gene            complement(5855..>6505)
                     /locus_tag="PLV49_00875"
     CDS             complement(5855..>6505)
                     /locus_tag="PLV49_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOG97424.1"
                     /translation="DVMQLRPEDLGSAALVEERRVGKEKQMIFVEGCEKARAISIILH
                     GVSDQLLEEMERALDDSLNVVMDVIRSGKIVPGGGAPEILVAENLRQYASTLEGREQL
                     AIRAFADAVEAIPFTLAENSGFDPVDSLAALRAKQGEGKIYGLDIASGKPADMMAQGV
                     VEPLKVKTQAIKSAAEAATMVLRVDDVIAAKREEMTPKPGQSPHDYTMPQMPMPHY"
     assembly_gap    6506..6605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6606..7261)
                     /locus_tag="PLV49_00880"
     CDS             complement(<6606..7261)
                     /locus_tag="PLV49_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOG97425.1"
                     /translation="MAGLSGVPVIILKEGSERESGKNAQHRNILAAKAVAEAVRTTLG
                     PKGMDKMLIDGSGDATITNDGATILREMDIENPVAKMIVEVAKAQDDEIGDGTTTAVI
                     IAGKLLEKAEALLEQDVHPTVIVQGYKQAAAKAQEVLKKMAIDVSGDQEMLLKIARTS
                     IRGKGTEMALDRLSQISVDAARAVVGFEGKDIEENIKMVHIPGGRIEESSINYGIVLE
                     "
     gene            7441..8187
                     /gene="pstB"
                     /locus_tag="PLV49_00885"
     CDS             7441..8187
                     /gene="pstB"
                     /locus_tag="PLV49_00885"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOG97426.1"
                     /translation="MTSKIVSENLNLWYGEKQALKDISLKIPEKQITALIGPSGCGKS
                     TFIRCLNRMNDLVTSVRIEGKVFYDDMDIYGKDTDVVELRKRIGMVFQKPNPFPMSIY
                     DNIAYGPRIHGQKNVSKIVERSLKESALWEEVSERLDQPALGLSGGQQQRLCIARTLA
                     MRPDVILFDEPCSALDPISTGKIESLMESLKEEYTQIIVTHNMQQAARISDITAFFLL
                     GELIEVGETKHVFEMPEMKSTEDYITGRFG"
     gene            8209..8862
                     /gene="phoU"
                     /locus_tag="PLV49_00890"
     CDS             8209..8862
                     /gene="phoU"
                     /locus_tag="PLV49_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOG97427.1"
                     /translation="MIPYRQRYRKDLSDLKSLTKDLADHVGEAIEKSVIALSDSNVDL
                     AREIIKNDQEVDDLSIKIENLCMELLVLQQPMARDLRRIIGILKIGIDLERIGDLAVD
                     IARVAAQSHNEITKLEFIPRMAEISRKMLRESMVALMDDDADTARQITKWDYEIDGLY
                     VKARDKLLKIIIDRPEVIKEGTSLLMVNRHLERTGDHICNICETIVYIVEAKREHLN"
     gene            8870..9874
                     /locus_tag="PLV49_00895"
     CDS             8870..9874
                     /locus_tag="PLV49_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOG97428.1"
                     /translation="MDTRKVQRTGKSTFIVSLPKIWATKNGIAAGSIVYINQGDNGAL
                     TLSTDRSERDLRVKLDIGEKTGDDLIRDIIGCYVGGYRIIEVTSPHMSSAQKKDLHQI
                     VNKLIGPEILEETINKVVIQDLLSSEELQSEKALRRIRTVVKSMIHDSFTSLLNNNQD
                     ELAMDVIQRDDDVDRLNLLISRQFTEILRTGSVKQETQNPIHAFNYMQAASNLERIAD
                     HAHRIAQIAREYNCTLADDLKEELMDMEVQLCGFIDDAISYLLQTNSDKANEMIDLIR
                     EIQKSAARITVTSDNLSKSEMLERLVLAASLERMFDYIMNIGELTINLSHSTKQAEKG
                     "
     gene            complement(9903..10346)
                     /locus_tag="PLV49_00900"
     CDS             complement(9903..10346)
                     /locus_tag="PLV49_00900"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOG97429.1"
                     /translation="MTKGAFVTALDLYIIVECSGQKVKRIYLSQESPDEPSHLAERIA
                     AHLEKGAPCPSPDLDLSRSTDFQKRVYSVVRGIGRGKTLTYGEVAFLAQCPKGARAVG
                     SAMATNPFAILVPCHRVVARKGLGGFAWGLEVKERMLALEREMTE"
     gene            complement(10343..>10555)
                     /locus_tag="PLV49_00905"
     CDS             complement(10343..>10555)
                     /locus_tag="PLV49_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG97430.1"
                     /translation="LASQLYDVLVRGHNHIFEVSSHGRTLLVNPGEIWGHLTGYSTVA
                     ILDTASMEVERVELGRFKTYREIIEG"
BASE COUNT         2315 a         2892 c         2645 g         2503 t
ORIGIN      
        1 tcatttgata tctcacagat cttccatcag gagattatga acttattttt cacatagagg
       61 ctctatcaag acctcttcgg ggctcaatct catgtgaaac ctctgtgatt cccgtataga
      121 tctagagcag atggatgatt tcaagcaaag gggtgcttcg cagtgctctt cgctgccaga
      181 atatccactg gctttggctc tccattcgct gcgcgttcta tggcaatctt cgtggcctgg
      241 taattatatt cataaacctt atctttatct tttgcatccc actccacaaa tacagacact
      301 attataaaaa gatcgtccgc caactcctca ggcagtactc ccgactcgac gcaatccatc
      361 acagctcgtg ccactgctgc ctgcacaggg ccaaagacca tcattgcctg ctgggcattc
      421 tttatggtga ccttgttgac catcagggtc gagggcttaa ccggcagatt gggggcaagg
      481 acggccaaca atggcgtatg gccccgggat ggcgatgcaa gtgccctcac aaatgccttc
      541 tccaccgggc tatcccttgg tcccataacc agatctatat gcgctacctc gggcccctct
      601 ccgaccagtg cttcaccaac aagtaccctc tgaaaagatt gcgacataac caaccttttg
      661 gtctttttca tataaatctg taatctaagc agtcggccaa aaattatatc atcataatgc
      721 aatgcattta tgcattaatg cgagtgatcg taatcaacaa ctatggacag ttcaatcacc
      781 tcatcaggcg cagcatacgg gataaggtgg actgtgacct cgcctccaac cagacgccac
      841 cagaagatat cgatgccgac ggcttgatac tgggaggagg accatccttg gatcgcgccg
      901 gaaactgtcg cgattatctt ttgaagctgg atatccccat cctgggaata tgcctgggca
      961 tgcagatcat gggcaccacc tttggtggtc aggttacggc aggaagcatt ggaggctatg
     1021 cccaggtgga tgtggagctg atcgatgaag acgacatcct gaaaggcttt ccggcaaagt
     1081 tcaaaacctg ggcatctcat gccgaccagg tgtcactgct tccttctcag ttccagaggc
     1141 tcgccacctc caggatctgt gatatcgagg ccatgaagca tcgcaatagg cctctgtatg
     1201 gagtgcagtg gcatccagag gtggtgcaca ctgagcatgg tgaagagctt ctggataatt
     1261 ttataaacat atgcaaaaga tgattccgac ggagtcccgg ggggatgact gcacgcccag
     1321 aaccgtttct taagtgacgg gacgtccaat atggatgtgt gatcttcatg cccgataaga
     1381 atcttttgtt agcagatctt gaggaatcat acgccgcagc taggcgcctc tcggcccggt
     1441 ccctggccgg ccagatcgag aggatcggat ttcagtgcct gggctgcggc caatgctgcc
     1501 ggggcgatga taacagcgtg gttgtctttc cctttgaggt aagacgaatc atggcgaaga
     1561 gggggctgga ttggctggag gtagcccagc cgccggaaga gggcgaatgg gacaacaacg
     1621 gcaacttcca taccctggag tggaggctca aaaaagaggg attgcgctgc cggttttatg
     1681 atgaggggca atgcactatc tatggccagc gcccccttct ctgccgcacc tatccattct
     1741 acctggtgga gggcgagcta cgctgctcgg aatgccccgg cctggggatg agaataaatg
     1801 aggatgctgc ccaagagatc gccgggcagc tcatcttgcg ccatatcact gagattgtgg
     1861 aggcaatagc gctcaccaaa aaataccagg acttccagcg gggcgggtgc aaagagggca
     1921 actgctgcat catacatgat agcgagggag agcacaaaat cccccttttc actgcaccgc
     1981 gctcttaacc tcgtctacga tcttatcgat gaggcttttc tccttctcnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc cttctcagtg gaagatcgct tcgcctcgcc
     2161 gaactcctct gccagctcct ccaatagctg cctttgcctg gcggtgagag aggcgggagt
     2221 gttgatcttg acccgtacat gcagatcgcc tgagccggtg ccatgcagcc tgggcatgcc
     2281 ttttcctctc aagcggaaga ccgttccggt ctgagttcct gcaggcacct tgatcctggc
     2341 ccgaccgttc agggtgggaa cctccagatc cgctcccaag gcagcctgag tgaagctgat
     2401 gggagtctcc atgatcagat cgctatccaa ccgctggaag agtctgtgag gctggatatt
     2461 tatgactaca taaagatcgc cggagacgcc cttggggccg gcagaatccc cctggccgcg
     2521 aagcttgagg tgctggccgt cctccacgcc cgccgggatc ttgatattga tcttgcgggc
     2581 ctttcttgcc cgtcccgtgc cgctgcagtc ctcgcagggg ctggtgatga gctctcccct
     2641 accgccgcac ttgggacagg tggaggaggt gaccatctgg ccgaaggggg tgttttgggc
     2701 ccgggttacc tggcccgagc cccgacaggt gctgcaggtc acgggacttg tgccgggctt
     2761 agccccgctg ccctggcact tggggcagtt ttccgtccgc ggaacctcta tagtcgtctc
     2821 caggccgcgt gacgcctgct ccagtgtcag gtcgatatca tagcgcagat cccggcccct
     2881 ggccggcccc cgccgtcctc ccccaccgcc gaagaacata tcgaagatgc tctctccgcc
     2941 cgcgccaaag ccccgaagaa gatcctcgaa gttcactccg cggaacaggt cctcttgcga
     3001 gtactgactg ttgattcctg catggccgaa ctggtcgtac ttctgccgct tatccggatc
     3061 ggacaggacc gcataagcct cagaaagctc cttgaacatc tcctccgccc cgggatcatc
     3121 tgaccggtcg gggtggtgtt tcatggcgag cttgcggtag gcggtcttga tatccttctc
     3181 gctggcatcc ttggataccc caagcacatc gtagtagtcc ttcttatccg gcatggtaca
     3241 gtcagttcct tcttctggtt tccctgtctt ccagcaccct tttcttcctt atagtttatt
     3301 ctccaattta cttcttatcg tttactacct cgtaatctgc gtccaccgtc tgatcctgcc
     3361 ctgtggaatc ctgacccgcc tccgggcctg agccctggcc tgcctgtccc tgctgggcct
     3421 gctgatcctg cgccgccttc tggtacatgg cggcagacag ctcgtagatt gcgttctgca
     3481 ggctctccgt cttggccttg atctcggaga tctctccgcc ggtctgggcg ttcttcaggt
     3541 cgctgatggc cttctcgatc ttctccttct gctcgcttgt ggctatgtcg cctgcctctt
     3601 tgagcatctt ctccgaggca tagatcagag attcagcagc attcttgacc tcaatctcct
     3661 cttttctctt caggtcgtcg gcggcaaacc tctcagcatc cttgatcttg gcgtcgatat
     3721 cctgctggct cagcttgtgg ggagcggtga tggtgatcct ctgctctttc ttggtggcaa
     3781 gatccttggc tgagacatga atgatgccat tggcatcgat atcgaatgtg acctcgatct
     3841 gaggtatgcc tcttggcgca ggagggatgc cgaccagggt gaaccttccc aatgagacat
     3901 tatcctgggc catgggcctc tctccctgaa ggacattgac ctcaacgctg gtctggttat
     3961 cggcagcggt ggtgaatatc tggctctttc tggtgggaat ggtagtgttc ctctcgatca
     4021 gcttggttgt gacgcttccc agggtctcta tacccaaaga gagaggcgtt acatctaata
     4081 gcaatagatc cttgacctct ccacccagga cgcccgcctg aatcgctgcc cccatagcca
     4141 cgcattccat gggatccact cctcgttcga tatcctttcc aaggaagttc ttaacgaact
     4201 cctggaccat tggcatcctg gtcggtccgc ccaccaggat gatcttgccg atatcggact
     4261 tctccagctt ggagtcttta agagcctgga tcatgggacc acggcagcgc tccagcacat
     4321 ctgctaccag ctcctccagt ttggagcggg ctatggtcat gctcagatgc ttggggccgc
     4381 tggcatcggc ggtgatatag ggcagattga ggttggtctg caggacgctg gatagctcta
     4441 tcttcgctac ctccactgct tccctcaggc gctgcatggc catggagtcg ccacgcagat
     4501 cgactccaga ttcctgcttg aacctgtcca gcacatagtt ggtcagccgg tcatccatat
     4561 cccgaccacc cagctgggtg tcgcccgatg tggacaggac ctcgaaaact ccttcgccga
     4621 tctccattat ggtgacatcc agcgtgcctc cgcccagatc gaagaccatg atcttatgct
     4681 cgcccacctt atccaggccg aaggcgaggg cggctgctgt aggctcattt attatgcgaa
     4741 cgacctccag gccagcgatg gtgccagcat ccttcgtcgc ctgcctctgg ttgtcattga
     4801 agtaggcggg aacggtgatc accgctttat ccacgctgtc agccaaatag gtctcggcgt
     4861 cggtcttgat ctttcgcaag acctgggcgg agatatcctc aggggtgtac tccttgtcga
     4921 aaaccctgac cttgtagtcc gtgcccatct tcctttttat tccctggacg gttccatctg
     4981 gattggttac agcctgcctc tttgccggct cgccgaccag aacctgacca tctttggtga
     5041 aggcgacata ggatggaaaa gccttgccac cgatgcttgt cccttctgcg ctgggaatta
     5101 ttgtgggccg gccgccgatc agaactgctg ctgccgagtt gctggttcca agatcaatac
     5161 caataatttt tgacaagatt tcatctcctg aacttagtaa atgctactac tctgtaaata
     5221 tttaaaccta tcatcatatc tttccaattg tatctcaacc cgccctattt acagccttct
     5281 gccttcaggg gcttattcac agcgaccttg gagaagcgaa tgacccgaga gttgaagaga
     5341 tagcctttct gtatctcttc ggccactatg tcctcttcca gatcatcctt cttcacctga
     5401 aataaggctt catgccggta gggatcgaac ttcttgccaa ccgcttcaat gggaacgaga
     5461 ccttccgtaa agagtactcc cagaagctgc atatggagca ccttttggcc ctcgtcatgc
     5521 tttgccgcct gctcgaggct gtcgagcacg gggagaagct tttggacgag cttttctgat
     5581 gcatatctaa ctgtatcctc cttctcccga acagatcgtt tcatcagatt gtcgagatct
     5641 gcacggcagc gcatcaattg atcgaggcgc tcctcagaca ggatctgcat ctccaccagc
     5701 ttcccttcca gctcatctat tctggcagtt aaggagcttt catcctcctc ttcatcctga
     5761 gcgcttactg ctgtatcgtc atccatttgc tccacccgaa gtatcattgt tcatttgatc
     5821 aaaagggagg ataggcaaaa gccgcctggt tccctcaata gtgaggcatt ggcatctggg
     5881 gcatggtgta gtcatgaggg ctctgaccgg gtttgggcgt catctcctcc ctcttggcgg
     5941 cgatgacgtc atccacccga aggaccatgg tagcggcttc cgctgcagac ttaatggcct
     6001 gggtcttcac ctttaaaggc tcgaccactc cctgggccat catatccgct ggcttgccgg
     6061 atgcgatatc caatccatag atcttgccct cgccttgttt ggctctcaga gcggcaagag
     6121 aatccacagg atcgaatcct gagttctcgg ccagggtaaa tggaatggcc tccacggcat
     6181 cggcaaaggc tctgatggcc agctgctccc tcccttccag agtggaagcg tactgcctca
     6241 gattctcagc caccaggatc tccggggctc ctcctccagg gacgatcttt ccggaccgaa
     6301 tgacatccat gaccacattg agcgaatcgt ccagagccct ttccatctcc tccaagagct
     6361 gatcagagac cccatgaagg atgattgaaa tagctctggc cttctcgcag ccttcaacga
     6421 agatcatctg cttttccttg cccacccgtc tctcctctac cagggctgca gatcccaaat
     6481 cctctggccg cagctgcatt acatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnttttc cagcacaatg ccgtagttta tggatgattc ctcaattctg cctccaggaa
     6661 tatggaccat cttgatattc tcctcaatgt ccttgccctc aaatcccacc acggcccgcg
     6721 cggcgtccac ggatatctga gagagcctat ctagggccat ctcagtgcct tttcccctga
     6781 tagaggtccg ggcgatcttc aggagcatct cctggtctcc ggagacatct attgccatct
     6841 tcttcaatac ttcctgcgcc ttggcggccg cctgcttgta tccctgcaca ataaccgtgg
     6901 ggtggacatc ctgctccaag agtgcctccg ccttctccag cagctttccg gcaattatca
     6961 ctgcggtggt ggttccgtcc ccaatctcat catcctgggc ttttgccacc tctacgatca
     7021 tcttggccac gggattttct atatccatct ctctgaggat tgtagctccg tcattggtta
     7081 tagtcgcatc accgctgcca tcgatgagca tcttatccat gcccttcggc ccaagagtag
     7141 tccgcacagc ctctgcgact gcctttgctg ctaaaatatt cctgtgctga gcattcttcc
     7201 cgctctctcg ctcgcttcct tccttcaata tgattactgg tactccgctc aaacctgcca
     7261 aaccttagtc ctccccgact ttttcttagt acgagtttaa agttctatat aaagaattcg
     7321 cgtgattgct atcttcattg ttatttagct cagttaacat atagtctata taggaagaac
     7381 tatatagcga gtcctcaatc aaggatgcac atatgcagat aacgctaaaa gggatatgct
     7441 ttgacctcta agatagtctc ggaaaacctg aacctctggt atggggagaa gcaggctctc
     7501 aaggacatct ccctgaagat accggaaaag cagataactg ctctcatcgg cccatcaggc
     7561 tgcggaaagt cgacatttat tcgctgcctg aaccggatga acgatctcgt gactagcgtt
     7621 cgaatcgaag gcaaggtctt ttatgatgat atggacatct atggaaaaga tacagacgtg
     7681 gtagagctgc gcaagagaat aggtatggta ttccaaaagc ctaatccatt tcccatgtcc
     7741 atttatgaca acatcgccta cggcccacgg attcatggac aaaaaaatgt tagcaagata
     7801 gtggagcgca gcctgaagga gtccgccctc tgggaggagg tcagcgagag gctggaccag
     7861 ccggctctgg gcctatccgg tggccagcag cagaggttat gcatcgccag gaccctggcc
     7921 atgcggcctg atgttatctt gttcgatgaa ccctgcagtg cattagatcc gatctcaacc
     7981 ggcaagatcg agagcctcat ggagagcctc aaggaagagt acacccagat aatagtcact
     8041 cacaacatgc agcaagctgc caggatctct gacatcactg ctttcttcct tctcggcgag
     8101 ctgatcgagg taggagagac aaagcatgtc tttgaaatgc ccgagatgaa gagcaccgag
     8161 gattatatca ccggacggtt tggatgagtc ctggtgggga aggagcgaat gatcccttat
     8221 agacagcggt atcgcaaaga tctttcagat ctaaaaagct tgacaaagga tctcgccgat
     8281 catgtgggcg aggccataga aaaatccgtt atcgccctgt ccgattcaaa tgttgacctg
     8341 gcacgagaga tcataaagaa tgatcaggaa gtggacgacc tgagcatcaa gatcgagaac
     8401 ctatgcatgg agctcctggt gcttcagcag cccatggcca gggatctgag gaggatcata
     8461 ggaatcctga agataggcat agatttggag agaataggag atctggctgt ggacatagct
     8521 cgagttgccg cccagtccca taacgagata accaagctgg agttcatacc gcgcatggcg
     8581 gagatcagca gaaagatgct cagggaatcg atggtggccc tgatggacga cgacgccgat
     8641 acggcccggc agatcaccaa gtgggattat gagatcgatg ggctgtatgt caaggcaagg
     8701 gataagcttc tgaaaatcat aatcgatcgg cctgaagtga taaaggaggg gacctcgctg
     8761 ctcatggtga acaggcatct ggagaggacg ggagaccaca tctgcaacat ctgtgagaca
     8821 atcgtctaca tagtggaagc aaaaagggaa cacctgaatt aggaggttca tggacaccag
     8881 aaaggtgcag aggaccggca agtccacatt catcgtatcc ctccccaaaa tctgggccac
     8941 gaagaacggg attgccgctg gctcaatagt ctacatcaat cagggggata acggggcttt
     9001 aaccctatcc accgatcgct cggagaggga tctgcgagtg aagctggata taggggagaa
     9061 gacgggagat gatctgatca gggacatcat cggatgctat gttggcggct acaggatcat
     9121 tgaggtcaca agccctcata tgtcatctgc ccagaagaag gatctgcatc agatcgtaaa
     9181 caagctcatc gggcctgaga ttttagagga gaccatcaat aaggtggtaa ttcaggacct
     9241 tttgagctca gaggagcttc agtccgagaa ggccttaagg aggatccgga cggtggtcaa
     9301 atccatgatt catgattctt ttaccagcct cttgaacaac aaccaggatg aactggcaat
     9361 ggacgtgatt cagagggatg acgatgtgga ccggctcaac ctcctcatct cccgccagtt
     9421 caccgagata ctccggaccg gttcggttaa gcaggagaca caaaacccca tccatgcgtt
     9481 caactacatg caggcggctt caaacctgga acgcattgcc gatcacgccc ataggattgc
     9541 ccagatcgcc agagagtata attgcactct ggctgatgac ctgaaagagg agctgatgga
     9601 tatggaggtt cagctgtgcg gatttatcga cgacgccata tcttatctat tgcagaccaa
     9661 ctcagacaag gccaatgaga tgatagatct gatccgagag atccaaaaga gcgccgcgag
     9721 gataactgta acctcggata acctgagcaa gtcggagatg ctggagcgtc ttgtcctggc
     9781 tgccagcctg gagaggatgt tcgactatat catgaacatc ggtgagctga ccatcaacct
     9841 cagccactct acgaagcagg cggagaaggg ttgattgcgg aacagatcgc tctgcctcat
     9901 tttcattctg tcatttccct ctccagggcc agcatcctct ctttgacctc cagaccccag
     9961 gcaaagcccc ccaagccttt ccgggcgacg acacgatggc agggaacgag tatggcgaaa
    10021 ggatttgtgg ccatggcact tcccacagct cttgcgcctt tcgggcattg ggcaagaaag
    10081 gcaacctccc cataggtcag agtctttccc cgccctatcc cccgcaccac tgagtatacc
    10141 ctcttttgaa aatcagtgct tctggaaaga tccagatccg gagagggaca gggcgcaccc
    10201 ttctccaaat gagcggcaat tctctccgca aggtgagagg gctcgtccgg gctttcttgg
    10261 gagagataga ttcgtttgac cttctgcccc gagcattcca caatgatgta gaggtcaaga
    10321 gcagtgacaa aagcgccttt agtcatccct cgatgatctc cctataggtc ttaaaccggc
    10381 cgagctccac ccgctccacc tccatgctgg cggtgtccag gatggcgacg gtagagtagc
    10441 cagtgaggtg gccccagatc tctcccggat tgaccagcag ggtcctgcca tgactggaga
    10501 cctcaaagat gtggttatgc ccacggacga ggacgtcata gagctgggat gccag
//
LOCUS       DAOSGP010000014        10434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5404_length_10434_cov_17.949513, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000014 DAOSGP010000000
VERSION     DAOSGP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5404_length_10434_cov_17.949513"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLV49_00910"
     CDS             <1..252
                     /locus_tag="PLV49_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696205.1"
                     /note="Sliding clamp subunit. Responsible for tethering
                     the catalytic subunit of DNA polymerase to DNA during
                     high-speed replication. Proliferating cell nuclear antigen
                     homolog; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase sliding clamp"
                     /protein_id="HOG97431.1"
                     /translation="YMEAKGDIDSLKLKIPSTELLGMKPGEARSLFSLDYLQDMSKSI
                     SKAGEVTLEMGIDYPLRVTFKLGQSVDINYLLAPRIEQE"
     gene            249..1274
                     /locus_tag="PLV49_00915"
     CDS             249..1274
                     /locus_tag="PLV49_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase large subunit PriL"
                     /protein_id="HOG97432.1"
                     /translation="MKVSDYPFTQEAAEAVRSRNISLDSLLDKSLFQSVRSRAAERLR
                     GAVGGEAIPEGRASSESEQLFELLSYPLARVMVSCLNDPILLRRYALAEAKLAFRRMQ
                     HEKEGLVVLANDLGINPQGQGPWKIHFSEYIRAAHRMHSPKWKLTNRDLSEGYLTVTD
                     VELRRLLEEAIREKVLKGLPVSVDERICQGLEEYLAPVKRDLEALAAKQKIDLGEVKE
                     GAFPPCIKKMLSEVSKGINLAHSARFALVSFLLQINMTPDEVIALFATSPDFDQDRTR
                     YQVEHIAGATGTRYKPPACATMATYGNCPGEDDLCRRIRHPLSYYERRSIMLKPGAIS
                     AAKEGQT"
     gene            complement(1243..3012)
                     /locus_tag="PLV49_00920"
     CDS             complement(1243..3012)
                     /locus_tag="PLV49_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HOG97433.1"
                     /translation="MPKEFRSLISLQEAKSIISDHLPPAREKAVALGSSLGCILAEKV
                     ISSQDVPGFGRASMDGYAVISQDTIVAREDRPVSLRLAGSVPMGRRPEIEISQGEAAE
                     VSTGSMMPKGADAVVMIEYSLAQEGIVYIRRPAFVGENVQAAGSDISFGEAVLFPGTP
                     IAAREIGVLAALGRESVRVRSLDVGLASTGAELIPPGRELLIGQIYDINSYTIAAGVE
                     DCGARPRSYGILPDDKEQMTRTLLKMAEECDMILVSGSTSAGAGDMIYQVIEEVGELI
                     FHGVNLKPGKPTIFGIIRGKPCIGLPGYPTSALTVFAELAGPAIRSALGRGHSENKTA
                     GRLAGPLRTEGRQQMLAVGLSGDLVYPVDKGSGSITTLALADGVIEIPSGVEFLEGGS
                     PVQVRLFSPAQPPCLVVAGENSLFLERLAEDLPWRLMLLNTGSYRGRIYLEDGIADLA
                     AVSGPLEEAPEGEAKVVWSGKRELGLIYRDPSALVDPASQRIVGWPRDSAMKEAFEQA
                     LKGMGIGAPVYVRLAKTHTAVAAIIASDRADLGFGEKEAASQAGLGFKPVVEDELYLL
                     AGPEGLGNPWIKSLMSALPLPPI"
     gene            complement(3045..>4000)
                     /gene="aroC"
                     /locus_tag="PLV49_00925"
     CDS             complement(3045..>4000)
                     /gene="aroC"
                     /locus_tag="PLV49_00925"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOG97434.1"
                     /translation="DRRRPGQSRASTQRKEMDRVEILSGIFEGKTTGTPISMLIWNRD
                     ADSASYDLLRNRPRPGHADYAYQMKYGLRDHRGGGRSSARETAGRVAAGAVAKKLLQH
                     SGVEVAAFVTELGGIESRIDSVDLAELCARAEANPIRCPDPNAAMRMMEQLDLVRQEG
                     DSLGGMVTIIARGVPPGLGDPVFDKLDADLAKALMSLGAVKAVEIGAGIGCAGMRGSE
                     MNDPLRIEGGKIGFESNNAGGILGGISTGMDIVCRIAVKPTPSIAKKQHTVDLAAGMD
                     AEIEIKGRHDPAIPPRMVPVAEAMVAIVLADHLLRQRMSRL"
     assembly_gap    4001..4100
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4101..5279)
                     /locus_tag="PLV49_00930"
     CDS             complement(<4101..5279)
                     /locus_tag="PLV49_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG97435.1"
                     /translation="MAGPYRILIIEDDPEHAELIKMGFRRHDEFFLNFAATGENGLEM
                     ISQDRYDLISVDLVLPGIGGLDVLVRIRRLDPDIPVVMVSGHGTTEMAVVAFENRATK
                     YVVKSLESFKSLPYVFENLIQEARFKANERQMRECIERSERIHRNVVENALAGIYILQ
                     EGVFKLVNPRLGEIFGCSADSLLGMPFWQLITPDDIQCAIRLEKDRQSGVPIYESRVL
                     RKDGTCRWIEFRTMPIEHEGQRAVLGNLLDITDRKEREIEILRANRELEAMDRISEIF
                     FSSKEDLDKVLADSLAFIVSGAGGADVGGIFLQENRGLVLRALHGSVEVLIEFVNDIQ
                     AGCDLLGKPLVHEVKDGSRGCWVSAPIISGAQKKGALVLACEELECLHFLERASLRIG
                     Y"
     gene            5398..7536
                     /locus_tag="PLV49_00935"
     CDS             5398..7536
                     /locus_tag="PLV49_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HOG97436.1"
                     /translation="MRPVAAFIIISILLASSVQARVLIVDPGDSSDAKTISAAVFKAS
                     PGDTIQIQPGRYPGAIIDRSLEIYGQEGVSIEGPLSINAPGCEIYDLKINTSRSDASL
                     SLLSGDCRIVRCTISGEKAAVSSEGSNNTIANCSISSPAGIEIFAPDNRVLGCHIQGS
                     GIGVRINQTSGCWVEDCTITALQGVLIEDSDRNGVVNNTILTDGLGLVLTRSDGNVAS
                     GNNLSGAFVSGIDMLNSRFNDIAANRIEGGKVGISLRVTEYCNVTSNWCLGNERAGIY
                     GNEASGLMLAENDLIENGNGILLSASADNIIRSNLASHNVYGISLRGSAKNLLQDNLM
                     ELNRYNLRIDDGDGADLSLQDYYDQDIDRSNTVDGRPIYYLVGKSGMNVPEDCAFLGL
                     VSCRDISARNLTISNSSVGMLLVNSSGCRIQNSSIDLSEEGILLRASDDCIISDCRVR
                     ECNVGFDSQDSRGIQFVADHARDCAAEGFRVDGSLNLLLLKCNSTSSNSGISLHSSRL
                     CRVQDCSSARNAKEGLLLSLSHRCSLVGNQATSNDRGIALTGSNACTLESNFAISNQK
                     DGISIEQLSDAEVSGNNATNNGQGIFVQSSKRSKISGNVLCNNSRYGLRMSTSTSCNI
                     TGNEICSNQIAGINLVDCTDNFLYHNIIRDNLIQNAADNGNNHWDAGAEVGGNYWSDH
                     AVVGDPGSVPRTIPAQGQDLYPFQHPGGWS"
     gene            7536..7994
                     /locus_tag="PLV49_00940"
     CDS             7536..7994
                     /locus_tag="PLV49_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOG97437.1"
                     /translation="MDELDLELLKVAQDGLDLTERPYRVWGEVLGMGEEEIIYRLRAM
                     EEEGIIRRFAATIGHRALGILANALIAWRVPPEKVDEAGAIFAAAEEVTHCYERATAQ
                     DWPYNIYTMVHSRSREECLQIAERLSSRSGISDHIVLFSEKEYKKISARI"
     gene            8018..8719
                     /locus_tag="PLV49_00945"
     CDS             8018..8719
                     /locus_tag="PLV49_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOG97438.1"
                     /translation="MVAPDDCSYRPFLPLFLDMSQRLVVIFGGGKVAERKALLFSDYG
                     PVQVVSQSFTPKLLASKDRWELVECDLTADFKRYLQGAFIAIPATSNSQLNRSIEEEA
                     IRVGILVNRVEGRGEVVVPSIIRKGQISIAISTESPGLTRYLRLRLEEELTEDYQGMA
                     KLLGRLRPELKELIPEQEDRARAIRSILEDKEVWRLLGHSYEKAYMRARSHACQDERD
                     SLDAGYSPHRLHRRD"
     gene            8661..9908
                     /gene="hemA"
                     /locus_tag="PLV49_00950"
     CDS             8661..9908
                     /gene="hemA"
                     /locus_tag="PLV49_00950"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOG97439.1"
                     /translation="MSEIASMLVTHRTASIDEIERAWHGDVETILKWVMSQDRVEECA
                     VLKTCNRVEVYVVAPRGEKVLFDLAKKARVSSRIIDFHDHDESLLHLLRLASGLESMI
                     IGEDQILGQMKELTQIAMKAGTTGWMLETAFKKAIQVGKRVRKETRINERSVSVGSAA
                     VDLAEEILGDLKGKSVLVIGAGETGELISRALLSREIGSLAVTNRTLSSAVCLAESLG
                     GEAVPFDDMPCRLHIADVVISATSAPHYILLKSDIEMAMAGRSNKLLIIDIANPRDVD
                     QAAAEVSGVELHNIDSLKNISRENMMQRMAEAARVEEIITEELQLLRAKYKRKEAEEL
                     LARLYSQAELIKDQEVRKAMNKLSARHTLGEIEQQVLCDMSRSIVNKILSEPTKSLKS
                     AAERGDLEILKSLCELFRLEEKL"
     gene            9905..>10434
                     /locus_tag="PLV49_00955"
     CDS             9905..>10434
                     /locus_tag="PLV49_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOG97440.1"
                     /translation="MRRMRRLRVPGIREMVSETDLGIQNLVQPIFVDERISEPRMIES
                     MPGQYHQSIESVAAEAGALEDLGVPAVLLFGLPSTKDEMGSAAADPDGVIQKAVRAIK
                     ECTSLLVITDLCLCEYTSHGHCGLVRGERVLNDETLPLLGEVAVSQAQAGADIVAPSG
                     MMDHMVRAIRTSLDWEG"
BASE COUNT         2313 a         2980 c         2894 g         2147 t
ORIGIN      
        1 tatatggagg ccaaggggga tatcgactcc ctcaagctca agatccccag cactgagctc
       61 ttaggcatga agccaggcga ggccaggagc ctcttctctt tagactatct gcaggatatg
      121 tcaaagtcca tcagcaaagc aggagaggtc acgctggaga tgggcataga ctaccccctg
      181 cgggtaacct tcaagctagg ccagtcagtg gacatcaact atctcctcgc tccccgaatt
      241 gagcaggaat gaaggtctcg gactatcctt ttactcagga ggcggcagaa gcagtccgca
      301 gccgaaatat ctccctggat agccttctgg ataaatccct tttccagtcg gtgcgatcca
      361 gagcggcaga acgcctgcga ggagcggttg gcggtgaagc cattcccgag gggcgggcga
      421 gcagcgagtc tgagcagctc tttgaacttc tcagctatcc actggccagg gtcatggtct
      481 cctgtctgaa cgatcccatt ctcctgcgcc gatacgctct ggcagaggcg aagctggcct
      541 tccggaggat gcaacatgaa aaggaagggc tggtcgtcct ggccaacgac ctggggatca
      601 atcctcaagg gcaggggccc tggaagatcc atttttctga gtacatccgg gcagcacacc
      661 gcatgcatag ccccaaatgg aagctcacca accgcgatct gtctgaggga tatctcacgg
      721 tcaccgatgt cgagctcagg cggctgttgg aggaggcgat cagagagaag gtcctcaagg
      781 gtctgccagt atctgtggac gagaggatct gccagggcct ggaggagtat cttgccccgg
      841 tcaagagaga cctagaggcc ctggctgcca agcagaagat cgatttggga gaggtcaaag
      901 agggcgcttt tcccccctgc atcaaaaaga tgctctctga ggtctcaaaa ggcatcaacc
      961 tagctcatag cgcccggttt gctctggtca gcttcctctt gcagatcaat atgaccccgg
     1021 atgaggtgat cgcccttttt gccaccagcc cggacttcga ccaggatcgc acccgctacc
     1081 aggttgagca tatcgccgga gcgacaggaa cccggtacaa gccgcctgcc tgtgctacca
     1141 tggccaccta tggcaactgc cctggggagg acgacctctg ccggaggatt cgccatcctc
     1201 tcagctatta tgagagaagg tcaatcatgc tcaaaccagg agctatatcg gcggcaaagg
     1261 aagggcagac ataagcgatt ttatccaggg atttcccagg ccctcgggcc ctgccaagag
     1321 gtacaattcg tcctctacta ccggcttgaa tcccagtccg gcctggcttg ctgcctcctt
     1381 ttccccaaat cccaggtccg cccgatcgga ggcaatgatg gcagccacgg cagtgtgggt
     1441 cttggccagg cgcacgtaaa caggagcgcc gatgcccatc cccttcaggg cctgctcgaa
     1501 tgcctctttc atagcggaat ccctcggcca gcccactatc ctctgactgg ccgggtcgac
     1561 cagagctgaa gggtcccggt agatcagccc tagctctctt ttgccgctcc agacgacctt
     1621 cgcctcgccc tcgggagcct cttccaatgg gcctgaaacc gctgccaggt ctgcaatgcc
     1681 atcctccaaa tagatccgtc cccggtacga accggtgttc aagagcatca gcctccaggg
     1741 cagatcctct gccagccgct cgaggaatag ggagttctct cctgccacca ccagacaggg
     1801 gggctgggct ggcgagaaga gtcggacctg gaccggtgat cccccctcta agaactccac
     1861 tccggaggga atctctatca ctccgtcagc cagggccagg gtagtaatcg agccgctgcc
     1921 cttgtccacc ggatagacca gatccccaga taagcccact gctagcatct gctgccttcc
     1981 ttccgtgcgc agagggccgg ccagccgtcc ggcggttttg ttctcagaat gcccacggcc
     2041 cagggcagag cgtatggccg ggccggccag ttcggcaaag acggtcagag cgctggtggg
     2101 ataacctggc agtccgatac atggcttgcc ccggattatc ccgaagatgg tgggctttcc
     2161 cggcttgagg tttaccccgt gaaatatcag ttctccgacc tcctctataa cctgatagat
     2221 catatcccct gccccggcgg aggtgctgcc gctcaccagg atcatatcgc actcttctgc
     2281 catcttcagc agggttctcg tcatctgttc cttatcgtcc ggcagaatgc cgtagctcct
     2341 gggacgggcg ccgcagtcct ccacccccgc agctatggtg tagctattga tatcatagat
     2401 ctggccgatc aagagctccc ggccgggggg aattagctct gctccagtgg aggccaggcc
     2461 cacatccagg cttcgtaccc ggacgctctc ccttcccaga gcggccagaa cgcctatctc
     2521 acgggctgca atcggcgttc cgggaaagag taccgcctcc ccaaaggata tgtcgcttcc
     2581 agccgcctga acgttctccc ccacaaaggc tggcctgcgg atgtaaacta tgccctcctg
     2641 agccagggag tactcgatca tgaccaccgc atctgcacct ttgggcatca tggagccggt
     2701 ggagacctcc gctgcctcgc cctgtgagat ctcaatctca ggtcttctgc ccatgggcac
     2761 cgatcccgcc aggcggaggg atacaggccg gtcctctctc gctacgatag tgtcctgaga
     2821 gatgaccgca taaccgtcca tagaggcgcg gccaaagccg ggaacatcct ggcttgatat
     2881 caccttctcc gccaggatac agccaagaga cgagcccagg gcaacggcct tttcccgggc
     2941 tggtggcagg tggtctgaga tgatcgattt ggcttcctgt aatgagatga ggctgcgaaa
     3001 ctccttgggc atgccgcccc aataagcccc gaaaagatat aaaatcagag ccttgacatt
     3061 ctctgacgca gaaggtggtc tgccaggact attgcgacca tagcttcggc caccggcacc
     3121 attctggggg gtatggccgg gtcatgcctc cctttgatct ctatctccgc atccattccg
     3181 gcagctaggt ccacggtatg ctgctttttg gcgatggacg gggtgggctt gactgcaatc
     3241 cggcagacta tatccattcc agtggatatt ccgcccaaaa tgccaccggc attgttgctc
     3301 tcaaagccta tcttccctcc ctcaattcgg agaggatcgt tcatctcgct tccccgcata
     3361 ccggcgcagc caatacccgc ccctatctcc accgccttca ccgcccccag gctcatgagc
     3421 gctttggcca ggtcagcatc cagcttgtcg aagaccggat ctcccaggcc cggcggcact
     3481 ccccgggcga tgatcgttac catgcccccc aggctatcac cctcctgccg gacaagatcc
     3541 aattgctcca tcatcctcat tgcagcatta ggatccgggc agcgtatggg gttggcctcc
     3601 gcccgggcgc aaagctcagc caaatctacc gaatcgatcc tggactcgat ccctccaagc
     3661 tcggtcacaa aggctgcgac ctccaccccc gaatgctgca gcagcttctt ggccaccgct
     3721 cccgcagcca cccggccagc cgtctctcga gcggacgacc tgcccccgcc ccgatggtcg
     3781 cgcaggccat acttcatctg ataagcataa tcggcatgac ctggccgggg cctgttgcgc
     3841 aagaggtcgt agctggccga gtctgcatcc ctgttccaga tcagcatgga tatgggggtg
     3901 ccggtggtct ttccctcgaa gatgccggag aggatctcca cccgatccat ctcctttctc
     3961 tgggtggatg cccggctctg gcccggacgc ctgcggtcca nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn atatccgatg cgcaacgatg ctctttccag aaaatgcagg
     4141 cattcaagct cctcgcaggc caggaccagc gcccccttct tctgcgcgcc ggagattata
     4201 ggcgctgata cccagcatcc tctcgatcca tctttaacct cgtgaaccag aggcttgccg
     4261 agaaggtcgc agcctgcctg gatatcattg acgaactcga tcaagacctc caccgatcca
     4321 tgcagagccc tcagcaccag gcccctgttc tcctggagga atatgccccc gacatccgcc
     4381 ccgcctgccc cagagacgat aaaggccagg gaatcggcta gcaccttgtc cagatcctcc
     4441 ttggaggaaa aaaagatctc agatatccga tccattgctt ccagttcccg gttggccctg
     4501 aggatctcga tctccctctc ctttctatcc gttatatcca ggagatttcc caggaccgcc
     4561 ctctgtccct cgtgctctat gggcatcgtc cggaactcta tccagcgaca tgttccgtcc
     4621 ttgcgcagaa cccttgattc ataaatcggc acgcctgact gccggtcttt ctccagccgg
     4681 atcgcgcact gaatgtcatc aggcgtgatc agctgccaga agggcattcc taggaggcta
     4741 tccgccgagc aaccgaagat ctctcccagc ctcggattga ccagcttgaa gaccccctcc
     4801 tggaggatgt aaatgccagc cagggcattc tccacaacat ttctgtgaat tctctccgac
     4861 ctctcgatgc actccctcat ctgcctctca tttgccttga accgtgcctc ttgtatgagg
     4921 ttttcaaaga cataggggag gctcttgaag ctctccaggc tcttgaccac atatttagtc
     4981 gcccgattct cgaaggcaac cactgccatc tccgttgtgc cgtggccgga gaccattacc
     5041 acggggatat ctgggtccag ccttctgatc ctcactaaaa catccagccc tccaatcccc
     5101 ggcagaacca agtccacaga tatcagatcg tatctatcct gggatatcat ctccaggccg
     5161 ttctctcctg tggcggcaaa attcaagaag aactcatcat gccttcggaa gcccattttg
     5221 atcagctctg catgctctgg atcgtcctca atgatgagaa tgcgataagg ccctgccatt
     5281 agaaaccatc cttgtcaaga agaaggatgc tttatcagta ttttatatct ttctgccaca
     5341 gcccctgctg attcactgca ttaggtttaa taggcggcag gggcataaac aataacgatg
     5401 agaccagttg ccgccttcat tattatttcg attctcctgg caagctcagt ccaggcccgc
     5461 gtcttgatcg tcgatcctgg cgacagtagc gatgctaaga ccatttccgc tgccgtattt
     5521 aaagccagtc ccggagatac aattcaaatt cagcccggca gataccctgg agcaatcatc
     5581 gaccgaagcc tcgagatcta tggacaagag ggcgtctcca tagaggggcc gctcagcatc
     5641 aatgcaccag gatgcgagat atacgacctc aagatcaaca ccagcagaag tgatgcctct
     5701 ttaagcctgc tttcaggtga ctgtcggatc gtccgctgca ccatatcagg ggaaaaagcg
     5761 gcagtaagct cagagggtag caacaataca attgctaatt gcagcatcag ctcccccgcg
     5821 ggaatagaga tcttcgcccc ggacaaccgg gtccttggct gccacatcca gggcagtggg
     5881 attggggtaa ggataaacca gacctctggc tgctgggttg aagattgcac aatcaccgct
     5941 ttgcagggag tgctaatcga ggactcggat aggaatgggg tggttaacaa caccattctc
     6001 acagacggac tgggcctagt cctcacccgg tcggatggaa atgtggcatc gggaaataac
     6061 ctcagcgggg cttttgtgag cggcatagat atgctcaatt cccggtttaa cgatatcgcg
     6121 gcgaatagga tcgaaggggg caaggtgggc atcagcttga gagtgacgga atactgcaat
     6181 gtgacctcca attggtgcct gggaaatgag agggcaggga tctatggcaa cgaggcaagc
     6241 ggattgatgc tggcagagaa tgaccttatt gaaaacggca acgggatact cctctctgcc
     6301 tcagccgaca acatcatccg gtccaatctc gcctctcata acgtctacgg tatttcattg
     6361 cgcggatcgg cgaaaaacct cctccaggat aacctcatgg aattgaaccg ctacaacctg
     6421 cgcattgacg acggcgatgg agcagacttg tctcttcaag actattatga tcaggacata
     6481 gaccggagca atacggtcga cggcaggcct atctattatc tggtgggaaa atcggggatg
     6541 aatgtgccgg aagactgtgc ctttttggga ttggtatcat gccgggatat atctgctagg
     6601 aatctgacca tatccaatag cagcgtaggc atgctgctgg tgaactcctc tggctgccgc
     6661 atccaaaaca gcagcatcga tctatccgag gagggcattc tcctccgggc cagcgatgac
     6721 tgcatcatct ccgactgtcg ggtccgggag tgcaatgtcg gcttcgacag ccaggactcg
     6781 agaggaattc agtttgtggc tgaccatgcc cgagactgcg ctgccgaggg cttcagggtg
     6841 gacggctcgc ttaacctgct tctgctcaag tgcaactcca cctcatctaa tagcggcata
     6901 tcccttcaca gctcccgact atgcagagtg caagactgca gctcagctcg aaatgcaaag
     6961 gaagggctcc ttctgagcct ctcccatcgg tgttctctgg tgggcaacca ggccacctca
     7021 aacgatcggg gcatcgccct taccggcagt aacgcctgca ctcttgagtc taatttcgcc
     7081 atctctaatc agaaagacgg catatcaata gagcagctat ccgatgcaga ggtttcaggc
     7141 aataatgcca ccaataatgg tcaaggcatc tttgtccagt cgtccaagag gtcgaagatc
     7201 agcggaaacg tcctctgtaa caatagccgc tacggcctgc gcatgagcac cagcactagc
     7261 tgcaacatca ccggaaatga gatctgctca aatcagatag ctggcatcaa tctggtcgac
     7321 tgcactgaca acttcctcta tcataacata atcagagata acctcatcca gaatgccgcg
     7381 gacaatggca ataaccactg ggacgctggg gctgaagtgg gaggcaacta ctggagcgat
     7441 catgcggtag tgggcgatcc gggaagcgtg ccccgcacca ttcccgccca gggccaggat
     7501 ctttatccat tccagcatcc aggaggttgg agttgatgga cgagttggac ctcgagcttc
     7561 tcaaggtcgc tcaggatgga ttggatctga cggagaggcc gtaccgggtt tggggtgagg
     7621 tcttggggat gggagaggag gagatcatat accgtctccg cgctatggag gaggagggca
     7681 tcatccgccg ttttgctgcc accatagggc acagagccct gggaattctg gccaacgccc
     7741 ttatcgcctg gagggtgccg ccagagaagg tggatgaggc aggagcgatc tttgctgcag
     7801 ctgaggaggt cactcactgc tatgaaaggg caaccgctca ggactggcca tataatattt
     7861 acactatggt ccattctcgc agcagagagg aatgcctcca gattgcagaa cggctctctt
     7921 cgcggtccgg cataagcgac catatcgtcc tcttcagcga gaaggagtac aagaagataa
     7981 gcgcaaggat atgatgccaa tatgatgcag gagtcttatg gttgcaccgg atgactgctc
     8041 ttataggccc ttcctccccc tttttctgga tatgagccag aggctggtgg tgatcttcgg
     8101 tgggggaaag gtggcggaga gaaaggctct cctcttttcc gattacgggc cggtgcaagt
     8161 ggtcagccag agtttcaccc ccaagctcct ggcatcaaag gatcgatggg agctggtgga
     8221 gtgcgatctg acggctgatt tcaagagata tctgcagggc gcattcatcg ccattcccgc
     8281 caccagcaac tcccagctaa accggtcgat agaggaggag gcgatccggg tgggaattct
     8341 ggttaaccgg gtggagggca gaggggaggt ggtggtgcct tccattatca gaaagggcca
     8401 gatctccatc gccatttcta ccgagtcccc cggcttgacc cggtacctcc gcctccggct
     8461 ggaggaggag ctgaccgagg actaccaggg tatggcaaag cttcttggcc ggctgaggcc
     8521 tgaactgaaa gagctcatac ccgaacagga ggatcgcgcc cgggcaatca ggagcatcct
     8581 ggaggacaaa gaggtctgga ggcttttggg ccactcctat gaaaaggcct atatgagagc
     8641 ccgctcacat gcatgccagg atgagcgaga tagcctcgat gctggttact caccgcaccg
     8701 cctccataga cgagattgag agagcctggc acggtgacgt agagaccatt cttaaatggg
     8761 tgatgtctca ggacagggta gaggaatgcg ctgttctcaa gacctgcaac cgggtcgagg
     8821 tttatgtggt ggcaccgcga ggggagaagg tgctcttcga cctggccaag aaagcgcgcg
     8881 tctcatctcg aatcatcgat tttcatgatc atgacgaatc cctgctccat cttctccgcc
     8941 tggcctcggg cctggagtcc atgatcatcg gagaggatca gatcctggga cagatgaagg
     9001 agttgaccca aatcgcgatg aaggcgggaa caactggctg gatgctggag accgctttta
     9061 agaaggccat tcaggtgggt aagagggtgc gcaaagagac ccgcatcaat gagcgctcgg
     9121 tgtcggtagg ctctgcagct gtcgacctgg ctgaggagat cctgggtgac ctgaagggca
     9181 aatcagtcct ggtgatcggc gctggcgaga ctggggagct aatatcccgg gcactgctca
     9241 gccgggagat cggatcgctg gcagtcacca atcggaccct tagctcagcg gtctgtctgg
     9301 cggagagcct ggggggagag gcagttccat ttgatgatat gccatgccgc cttcatatcg
     9361 cagatgtggt gatcagcgcc acctctgccc cccattacat cctcctcaag agcgacatag
     9421 agatggccat ggccggcagg agcaacaagc ttctcataat cgatatcgcc aaccccaggg
     9481 acgtggacca ggctgcggcc gaggtttcag gggtcgagct gcataacatc gacagcctca
     9541 agaacatcag ccgggagaac atgatgcaga ggatggcaga ggcggcacgg gtggaggaga
     9601 tcataacaga agagctgcag cttctccggg ccaagtacaa gaggaaggag gcggaagagc
     9661 tgctggctcg gctttactct caggcggagc tgatcaagga ccaggaggtt agaaaggcca
     9721 tgaacaagct ctcagccagg cataccctgg gcgagatcga gcagcaggtc ctgtgcgata
     9781 tgagccgctc cattgtgaat aaaatattat cggagccgac caaatcgctg aagagcgctg
     9841 cggagagggg agacctggag atactgaaat cattgtgcga gctgttccgg ctggaggaga
     9901 agttatgagg agaatgaggc ggctgagagt gccggggatc agagagatgg tttccgagac
     9961 ggatctgggc atccagaacc tggtccagcc catatttgta gatgagagga tatccgagcc
    10021 gaggatgatc gaatccatgc ccggccagta tcatcagagc atagagagcg tggcagcaga
    10081 ggcaggagcg ctggaggatc tgggggtgcc agcggtgctg ctattcggcc tgccctccac
    10141 caaggatgag atgggctcgg ctgcagccga tcccgatggc gtgatccaga aggcggtgcg
    10201 ggcaatcaag gaatgcacca gtcttttggt cataaccgac ctgtgtctgt gcgagtacac
    10261 cagccacggc cattgcggtc tggtccgggg agagcgagta ctgaacgatg agacccttcc
    10321 cctgcttggc gaggtggcag tgagccaggc ccaggcggga gcggatatag tcgcccccag
    10381 cggcatgatg gatcatatgg tgcgggccat tcgcacctct ttggactggg aggg
//
LOCUS       DAOSGP010000015        10410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5424_length_10410_cov_20.126702, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000015 DAOSGP010000000
VERSION     DAOSGP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5424_length_10410_cov_20.126702"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLV49_00960"
     CDS             complement(<1..618)
                     /locus_tag="PLV49_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOG97441.1"
                     /translation="MQFATWEPFYNQILQDFGFSSARDEEGAHLLAELLQDSGDCRPS
                     AEERIRGRKAVIFGNAPSLDRELDALPCMDAARIAADGAAAVLLRRGIVPDVVVSDLD
                     GPFPAILEACQKKAIIVVHAHGDNLDALARYVPQLKNVIGTCQCRPSGGLYNFGGFTD
                     GDRSVFLAVELGASSIELVGFDFEDQSVTPRKRKKLAWAKRLIEIA"
     gene            complement(650..>1700)
                     /locus_tag="PLV49_00965"
     CDS             complement(650..>1700)
                     /locus_tag="PLV49_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97442.1"
                     /translation="AKGNQQIEIGDLTKRKEGIFNGNGNIRVDGGIDLKARLAALTAM
                     ILIAFTGNSLAATDNDINEGYKYTNAILQKNITFDFEQSVQGNGYFMTYIYAKAGNTA
                     VKNYAHGSGSLNSESLLTLQDMDRLTHDANADYNDYYQNCIEFKEDVSMVYAPMRIAV
                     GTGYYAANPLDYSSLLKEKTWVKNYRAGTSMHHEVEYAHALDKELNIVVKEVFNKTYD
                     PEIFGIGYTQMTINEDMTDGKAHIGVLQAADNYASLDDGDFTENLAIRKTAWRKPAIE
                     IDEDYWGTYHIEKNMTLETPYRLVQKNEDWLPCCFSGFDSMTSAEKKYIKSATGIFDC
                     TCFKAPTQAQFQRTY"
     assembly_gap    1701..1800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2094..2954
                     /locus_tag="PLV49_00970"
     CDS             2094..2954
                     /locus_tag="PLV49_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil protein"
                     /protein_id="HOG97443.1"
                     /translation="MLAELEEKRGKLRQESLAFKDRRSQLNAEASKWAAKRNELNKAT
                     KELIDKAQELKKLRDENNKNVAESKKHRDEFNEKTNQLYARIDDIRKKYNLTGDRSIR
                     DLRREIDHLEFRQQTEVLSPDKEKQLVDKIASLHAEFKSRKEQLEKNEELRKLLDEAQ
                     ALRDEASKHHDDVTKYAELAQEYHDQMIATFKEADQTRAEADAAHKEFVKAQEAADEQ
                     HKEFIRTQREVRDFEKVIMGLKKKNKDIKEDRAKEVAKREAEEILTHFRQGEKLNTAD
                     LLRLQRAGLV"
     gene            3143..3652
                     /gene="cgi121"
                     /locus_tag="PLV49_00975"
     CDS             3143..3652
                     /gene="cgi121"
                     /locus_tag="PLV49_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HOG97444.1"
                     /translation="MNEIRLLFGRPAIPDRKDLISAIKDLQHRYGCIVQALDADKVVS
                     NRHILFATEKALSAFSQRRNIAKDVGMEIMRYASGERQIERALNMGVSDKTERIALVL
                     ASLEGQCNWPDEIELSRLLKPDGLGCSCRYNAVKEVFNISSAELDSVGEDRIEDLVIE
                     RVALADTYR"
     gene            3669..4280
                     /locus_tag="PLV49_00980"
     CDS             3669..4280
                     /locus_tag="PLV49_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2150 family protein"
                     /protein_id="HOG97445.1"
                     /translation="MSSTMKEEFYTQKRWLNWMNKVKDSNFKLTESDDKNSGAVFIYI
                     MDDVVLACLKVIARFEREIISAEEALDDIEAIRSIVFQEHESLGEDADLMIESLKTSL
                     AAVFVSSQRYITGSFDRESPLDELVARAVAAEEKENLDEAFDLLSQVGARVIGGENLP
                     EIGELPYCMTAELMDGIDAISAAMIGDDSYKDEDGSEDTGEGD"
     gene            complement(4340..5224)
                     /gene="map"
                     /locus_tag="PLV49_00985"
     CDS             complement(4340..5224)
                     /gene="map"
                     /locus_tag="PLV49_00985"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HOG97446.1"
                     /translation="MMDAEILDNYRKAGRILAEVLQEAKPKVDVGVPLLEVAEFVEEA
                     IRSKGGLPAFPCNISLDRSAAHYTPSPKDESVFAENMVKLDVGVHVDGYIADAAITID
                     LSGHEDLVLASRAGLDAALELVGPGVSTADIGKAIEEAIVGYGYKPVYNLTGHGLSRY
                     IAHDLPAVPNKRMDKGVTLKEGDVIAIEPFATNGSGRISEAPITEIYGFSGSRPQRLP
                     AARALMKEIVESYKTLPFARRWLKAERAEYALMHLVRSGAVHGYPVLWEVEGALVSQA
                     EHTVLILENGCEVTTRIG"
     gene            complement(5272..6417)
                     /locus_tag="PLV49_00990"
     CDS             complement(5272..6417)
                     /locus_tag="PLV49_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOG97447.1"
                     /translation="MSSDILRFLDSSNIDGFLFVGDSISNSDMYYLSHFLAGDRFAIL
                     FQEKTSILISSMEKGRAKKEACADEVLSTRDYHIMEKLKSCGKPELAYIEALKDFLRD
                     HGVLRLGVAYSFPIGIFQPLSRHFDIQILESPARRARSKKSPQEIDCITTAQRACERA
                     MQRAVELIACSEPRGDILYLQGQPLTAEQIRSAIDISLLEDGCEAEDTIVAGGTQAAE
                     PHARGTGPLPANSPIVIDIYPRSKTNRYYADMTRTVLRGEAPGEVKELYHAVLAAQEA
                     GIGAIRSGISGEEVHAAVSAVFQEMGYSERDGCGFTHSTGHGVGLDVHELPSLSEGGE
                     ILKSSQVVTVEPGLYYPDIGGVRLEDLLVVRDKGCENLTRCRKDLVL"
     gene            complement(6424..7182)
                     /locus_tag="PLV49_00995"
     CDS             complement(6424..7182)
                     /locus_tag="PLV49_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOG97448.1"
                     /translation="MTFEMGRVELHLRDAVKKHGAGHLTLIDPDSQTPQEAGRMAQAA
                     SQGGTDAIMVGGSVGATGALLHQTVAEIKKVTDLPVILFPASVAGLCENADAVFFMSL
                     LNSRSPNYLIENQAMGAPLVLRYRLEALPMAYIIIEPGGTVGYVGDARLIPRKKPELA
                     CAYALAGKYLGMRLVYLEAGSGADSPVPASMVALTKKLLGDVLIIVGGGIRSGAAAAE
                     LVAAGADLIVTGTAVEKSQDVASFISELTSSIRR"
     gene            complement(7190..7339)
                     /locus_tag="PLV49_01000"
     CDS             complement(7190..7339)
                     /locus_tag="PLV49_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HOG97449.1"
                     /translation="MARFPEAENRILNLKICMRCNARNPVKATRCRKCGYEGLRMKSK
                     ENKTA"
     gene            complement(7395..7545)
                     /locus_tag="PLV49_01005"
     tRNA            complement(join(7395..7408,7440..7490,7524..7545))
                     /locus_tag="PLV49_01005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7476..7478),aa:Ser,seq:cga)
     gene            7617..>8749
                     /locus_tag="PLV49_01010"
     CDS             7617..>8749
                     /locus_tag="PLV49_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOG97450.1"
                     /translation="MSLREEALAFHEAVMGKIAVHSKVPCATSRDLSLAYTPGVAAPC
                     REIERNPDDVYRYTAKGNLVAVVTDGTAVLGLGDIGALASIPVMEGKAILFKNFAGID
                     AFPIAVASKDPEDIVRTVAMIEPVFGGINLEDISAPRCFEVEERLKKALTIPVFHDDQ
                     HGTAVVALAALINALRLVGKDFRDLKVAVSGAGAAGVAVTKFLLSFGAKDVILCDSRG
                     VIHKARPDLNPSKQEIAALTNPRNVTGTLADAIRGADVFLGLSVAGIVTPDMVSSMAG
                     DAVVFAMANPEPEIMPELALEAGAVVVATGRSDYPNQVNNVLGFPGIFRGALDVRASD
                     INEQMKMAASRAIAGLVENVTRECIIPSPLDRRVVPAVAEAVAR"
     assembly_gap    8750..8849
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8951..9934)
                     /locus_tag="PLV49_01015"
     CDS             complement(8951..9934)
                     /locus_tag="PLV49_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOG97451.1"
                     /translation="MSGQGSKRVAVIAGDGIGPEVVESALEVLRAAGASLDLVHFEIG
                     LGRWKRTGEAMDEDDLEEISGCDCILLGAITTPPDPNYRSVLLRLRRALDLYANIRPF
                     QSHDLDFIIVRENTEGLYSGVEEVGVEESRTLRVITRRGSERIAEAACDLAARRRRLT
                     IIHKSNVLRSDRLFLQTCREVAERRGILYEDMLVDAAAYNLVTNPQRFDVLVTTNLFG
                     DILSDEAAGVVGSLGLCASANLGKSRALFEPIHGSAPDIAGKGIANPVGAIRSAAMMM
                     RWLGELEAAGRIEEAVKRALAAGVRTPDLGGRCSTSEVTGAVAGYLMAEES"
     gene            complement(9907..>10410)
                     /locus_tag="PLV49_01020"
     CDS             complement(9907..>10410)
                     /locus_tag="PLV49_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97452.1"
                     /translation="PDPGQVLKIEVMDVSPPDPPWLICTLQGLEDCGVLGDLMVRFVP
                     RILNLERFYCPSVYYPCRAGGLGRSLDCDLVVHERPRIVGCEVSREIFLANNPGKEHE
                     FINVCPIHCREREFQPQGPFITRCCRSERRGRTEKCGQPGIVVHWGDGAWEIAEAVRC
                     LVKDLRG"
BASE COUNT         2268 a         2875 c         2741 g         2326 t
ORIGIN      
        1 ggcgatctct attagcctct ttgcccaggc cagcttcttt ctttttcggg gggtcacgct
       61 ctggtcctca aagtcaaagc ccaccagctc aattgaggac gccccgagct cgaccgccag
      121 gaatacggac cgatctccgt ctgtgaatcc tccgaagttg tacagtccac ctgatggccg
      181 gcactggcag gtgccgatga cattcttcag ctggggtaca tatctggcta aggcatccag
      241 attgtctccg tgagcatgaa cgacgatgat cgctttcttc tggcaggcct ccagaattgc
      301 aggaaagggg ccgtccaggt cggaaaccac aacatccggc acaattcccc ttcttaagag
      361 aactgctgcc gccccgtccg cagcgatgcg ggcagcatcc atacagggca acgcatcgag
      421 ctcgcggtca agggatggag cattgccaaa tatcaccgcc tttctcccac gaatccgttc
      481 ctctgccgat gggcgacaat ctccggaatc ctgcaaaagc tctgccagta gatgcgctcc
      541 ctcttcatcc ctggcagaag agaagccgaa gtcctgcaat atctgattgt agaatggctc
      601 ccacgtagcg aactgcatat taaaaaaaag cggagagttt ctccctcact tagtaggttc
      661 tttggaactg ggcctgggtt ggtgccttaa agcaggtgca atcaaagata ccggttgcag
      721 acttgatgta cttcttttcg gcagaggtca tgctgtcaaa gccgctgaag cagcagggca
      781 gccagtcctc attcttctga accagacggt agggagtctc cagcgtcatg ttcttttcga
      841 tatgataggt tccccagtag tcttcgtcta tctcaatcgc tggctttctc caggcagttt
      901 tcctgatcgc gaggttctcg gtaaagtctc catcgtccag gctggcatag ttgtctgcgg
      961 cctgcagaac accaatgtga gcctttccgt cggtcatatc ctcatttata gtcatctggg
     1021 tatagccaat gccgaagatc tccggatcgt aggtcttatt gaagacctct ttgacgacga
     1081 tgttcaactc cttatccagg gcatgggcat actcgacctc atgatgcatg ctggtgccgg
     1141 cgcggtagtt ctttacccag gtcttctcct tgagcaggga gctgtagtcc aggggatttg
     1201 ctgcataata tcctgttcca actgcaatcc tcatgggcgc gtagaccatg gagacatcct
     1261 ctttgaactc gatgcaattc tgataataat cattgtagtc tgcgttggca tcgtgggtaa
     1321 ggcggtccat atcctgtaat gtcaagaggg actcgctgtt caatgatcca ctgccgtggg
     1381 cgtagttttt gactgcggta ttgcccgctt tggcataaat ataagtcatg aagtagccgt
     1441 taccctgcac gctctgctcg aagtcgaatg tgatattctt ttgcaggata gcgttggtgt
     1501 atttataccc ctcattaatg tcattatcgg ttgctgctaa tgaattccct gtgaaagcaa
     1561 tcaaaatcat tgctgtcagc gctgctagtc tggccttcag atcaatccct ccatctacgc
     1621 gaatattgcc atttccatta aatattcctt cttttctttt ggtcaaatca cctatttcaa
     1681 tctgttgatt gccttttgca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 tttctcgata ggtattaata tgctcgctcc tgttgatctt atggaaaggc catttttgat
     1861 cataccgctt gcatcaaaca actgagcagt ggaataatct gggatgagaa tggtgcacct
     1921 ttgtgcactg gcctggcaat tccatttgat gaacgagttg ggcgtataaa aaacccatat
     1981 gtatcgcgcc atagaaaagc gcttcgccat ccaggcgaag gattcccggg ttttttggtt
     2041 ccggagcggg accaaacccc agccgtgggc tggaacagga ggataaacgg gggatgctgg
     2101 cagaactaga agagaaaaga ggcaaactca ggcaggagtc ccttgccttt aaggatagac
     2161 gcagccaact gaacgcagag gcgagcaagt gggcagccaa aagaaatgag cttaacaagg
     2221 ccaccaaaga gttgatcgac aaggcccaag agctcaagaa gctgcgggat gagaataaca
     2281 agaatgtggc tgaatccaaa aagcacaggg acgagttcaa cgagaagacc aatcagctct
     2341 atgccaggat agacgatatc cgcaagaagt acaaccttac tggcgatcgt tcgataagag
     2401 acttgcgccg ggagattgat catcttgagt ttaggcagca gactgaggtc ttgagcccag
     2461 ataaagagaa gcaactggtg gacaagattg cctccctgca tgcggagttc aagagtcgca
     2521 aggagcagct tgagaagaac gaggagctga ggaagcttct ggatgaggct caagcattga
     2581 gggatgaggc atcaaagcac cacgatgacg tcacaaagta cgcagagctg gcccaggaat
     2641 accatgatca gatgattgcg accttcaagg aagccgatca gacccgtgcg gaagcagatg
     2701 ccgctcacaa ggagtttgta aaggcccagg aggctgcaga tgagcagcat aaggagttca
     2761 tccgcaccca gcgagaggtt cgcgacttcg aaaaagtgat catgggcttg aagaagaaga
     2821 ataaggatat caaagaggat cgcgccaaag aggttgccaa gcgcgaggca gaagagatcc
     2881 ttacccactt ccggcagggc gaaaagctga atacggcaga tctgctaagg ttgcagcgtg
     2941 ccggcctggt ctgaaaggct cgaaaatttc cacataattg ggcagataaa actgcccttt
     3001 ctcagatttt agcatttctt gatttatgct tgggcttgcc cgatgttttc gatccagtca
     3061 agtggcagcg ttttgcccat tctgctggga atcgatttac aggaccgcaa gcttttagag
     3121 cagcagatcc gatctcggag tgatgaacga aatccgcctc ctcttcggaa ggccggccat
     3181 ccctgataga aaggatctca tatcggcaat aaaggatctg caacatcgat atggttgtat
     3241 tgtgcaggct ctggatgcag ataaagtggt aagcaatagg catatactct tcgccacgga
     3301 aaaggcctta tccgccttct ctcagagaag aaacatcgcc aaggatgttg gcatggagat
     3361 aatgagatat gcttccggag agaggcagat tgagagggcc ctaaatatgg gtgtatcaga
     3421 taagaccgag cgcatagccc tcgtcctcgc cagcctggag ggccaatgca actggccaga
     3481 tgaaatagag ctgtctagac ttctaaagcc ggatggactg ggctgctcct gccgatacaa
     3541 tgcggtaaaa gaggttttta atatctcttc tgcagagctt gattcggtgg gagaggacag
     3601 gatagaggat ctggttatcg agagagttgc cctggcggat acataccgct gaattgtata
     3661 gagaaaggat gagttctaca atgaaggaag aattctatac ccagaagcgt tggcttaact
     3721 ggatgaacaa ggtgaaggac agcaacttca agctcacaga atccgatgat aagaactcgg
     3781 gagcggtctt catctatatc atggacgatg tggttttggc ctgtctcaag gtcattgccc
     3841 gctttgaaag agagatcata agcgctgaag aggctttgga cgatatcgag gccatccgtt
     3901 caattgtctt ccaggagcac gaatctcttg gagaggatgc cgatctgatg atagaatcgc
     3961 tcaagacctc gcttgctgcg gtattcgtct ccagccagcg ctatatcacc ggaagcttcg
     4021 atcgggagag ccccctcgat gagctggtgg caagggcagt agcggcagag gagaaggaga
     4081 acctggatga ggccttcgat ctgctcagcc aggtgggggc cagggtgatc ggtggggaga
     4141 atcttcccga gatcggcgag ctgccttatt gtatgactgc cgagctgatg gacggcattg
     4201 atgccatctc cgcggccatg ataggcgatg acagctataa ggatgaggac ggatcggagg
     4261 atacaggtga gggtgattag tctcgcttaa ttctgccctg tttttccttc gtgagaaaat
     4321 aaaggccaaa aagaagcctc tagcctatcc tggttgtcac ctcacatcca ttttccagaa
     4381 tcaggaccgt atgctctgcc tgggagacca gcgctccctc aacctcccat agtaccgggt
     4441 atccgtgcac agcaccgctc cgcacgagat gcatcaatgc atattccgcc ctctcggcct
     4501 tcaaccaccg tctggcgaaa ggtagcgtct tatagctctc cacgatctcc ttcatcaggg
     4561 ctctggccgc gggaagcctc tggggccggg acccgctgaa tccatagatc tcagtgatgg
     4621 gcgcctcgct gatcctccct gagccattgg tggcgaaggg ctcaatggcg atgacatccc
     4681 cctcttttag ggtcactccc ttatccattc tcttattcgg caccgctggc aggtcatgag
     4741 cgatatagcg agagagtccg tgcccggtca gattgtagac cggtttgtag ccgtagccaa
     4801 ctatggcttc ctctatcgcc tttccaatat cagcagtgct cacccccggc ccgaccagct
     4861 ccagggcggc atccagacct gcccgggagg cgaggaccag atcctcgtgg ccgctaaggt
     4921 ctatggtgat ggcagcatcg gcgatgtagc cgtccacatg cactcccaca tccagcttca
     4981 ccatattctc ggcaaatacg ctctcgtcct ttggcgaggg tgtataatga gccgcgctcc
     5041 ggtccaaaga tatgttgcag ggaaatgcag gcaggccgcc ctttgacctt atggcctcct
     5101 ccacgaactc ggccacctcc agcaggggca cgcctacatc caccttcggc tttgcctcct
     5161 gcagcacttc ggccagaatt cgtccggcct tccggtaatt atctagaatt tctgcgtcca
     5221 tcattatcac caaatggcaa gccagctata aatgctctgt cgagctcttt ttcagaggac
     5281 gagatccttt ctgcagcggg tgaggttctc gcatccttta tctctcacta ccagaagatc
     5341 ctccagtctc actccaccga tatctggata gtataggccc ggctctacgg tcactacctg
     5401 cgagcttttc aatatctccc ctccttcgct cagggagggc agctcatgca catccagccc
     5461 cacgccgtgc ccggtggaat gggtgaagcc acagccatcc ctctccgaat agcccatctc
     5521 ctggaagaca gcggagactg cggcatggac ctcctctcct gatatgccgc ttcgaatggc
     5581 accgatccct gcctcctggg cggcaagaac ggcatggtag agctctttta cctccccagg
     5641 agcctctcct cgaaggacgg tgcgagtcat atctgcgtag tagcggttag tcttggaccg
     5701 gggatagatg tcgattacaa tgggggaatt ggccggcagg ggacccgtcc ctcgcgcatg
     5761 gggttcggca gcctgggtgc cgccggcaac gatggtgtct tcggcctcgc agccatcctc
     5821 aaggagggag atatcaatgg ccgatcgaat ctgctcagcg gtaaggggct ggccctgaag
     5881 gtagaggata tctcctcgag gctcagagca ggcgataagc tccactgctc gttgcatcgc
     5941 cctctcgcag gccctctggg cagtggttat gcaatcgatc tcctgggggc tcttcttcga
     6001 ccttgcccgc ctggccgggc tctccaggat ctggatgtcg aaatgcctgg ataaaggctg
     6061 gaatatccct atcgggaagc tgtatgcaac tcctaagcgc aatacgccgt gatccctcaa
     6121 aaaatccttc aaggcctcta tgtaggccag ctccggcttt ccgcagctct tcagcttctc
     6181 catgatatgg tagtcccggg tgcttagaac ctcatctgca caggcctcct tctttgctct
     6241 gcccttttcc atggaggaga taaggatcga agtcttttcc tggaagagga tggcgaacct
     6301 gtctccagca agaaaatggg agaggtaata catatccgag ttgcttatgc tatctcccac
     6361 aaagagaaag ccatcaatgt ttgatgaatc gagaaatctc agaatatccg atgacacgaa
     6421 gtgctacctg cgaatggatg aggtcagctc agagataaaa gaggccacat cctggctctt
     6481 ctcaacagcg gtgcccgtga ctatcagatc ggctccggcg gccaccagct ctgcggctgc
     6541 tgcaccgctt cttatgccac cgcccacaat gatcagcaca tcgcccagca gcttcttcgt
     6601 cagtgccacc atgctcgcgg gcaccggcga gtccgcccct gaaccggcct ccagatagac
     6661 cagcctcatt cccaggtact tccccgccaa agcgtatgca caagccagct ccggcttctt
     6721 tctgggtatc aggcgggcat cgcccacata gcccaccgtc ccaccgggct cgatgatgat
     6781 ataggccatg ggcagggcct ccaggcggta gcgcagcacc agaggcgctc ccatagcctg
     6841 gttctcaatg aggtagttgg gagagcggct atttagcagg ctcatgaaga agactgcatc
     6901 ggcgttctcg cacagcccag ctacacttgc cggaaagagg atgaccggaa gatcggtcac
     6961 cttcttgatc tcggccacgg tctggtgcag gagggcaccc gtcgccccta ccgagccacc
     7021 taccatgatg gcgtccgtcc ccccctggct tgccgcctgg gccatcctcc ctgcttcctg
     7081 gggggtctgg gaatcaggat cgataagggt taagtggccc gctccgtgct tttttacagc
     7141 atccctcagg tgcagttcta ccctgcccat ctcaaaagtc atttttgatc taagcggtct
     7201 tgttctcctt ggacttcatc ctcaggcctt cgtagccgca tttgcggcag cgtgtagctt
     7261 ttaccgggtt tcgcgcattg cagcgcatgc atatcttgag gttcaagatt ctgttttctg
     7321 cttctggaaa tctagccata ataatcagct cttcgtcgca acttactctg atatgaaact
     7381 ttgcatattt ttagcgccgg ggttgggatt ttcctggcgg gtctcttgcg ccccgtcatt
     7441 ttgaacccaa gtgtccctgg aagggacaac aggtctcgag cctgccgcgt ttgaacttcc
     7501 gccggccttt agccgcccgg aatttgtccg ctctgccacc ccggctcagg ttagacaccc
     7561 tctggcccat gctttatatc tgttgctccc ctgaatgcct cccacaggag tatgttatgt
     7621 cccttcgaga agaagctcta gcctttcatg aagccgtcat gggaaagatt gccgtgcaca
     7681 gcaaggtgcc ctgcgccacc agccgggatt tgagcctggc ttataccccg ggagtggccg
     7741 caccctgtcg cgagatcgag aggaatccgg atgacgtcta tcgctacact gccaagggaa
     7801 atctggtggc agtggtaacg gacgggactg ccgtgctggg attgggcgat ataggagctc
     7861 tggcctccat tccggtgatg gagggcaaag cgatactgtt caagaacttc gccgggatag
     7921 atgcctttcc aatagctgtc gcctctaagg atcctgagga tatagtgagg accgtggcca
     7981 tgatcgagcc ggtcttcgga gggataaacc tggaggatat cagtgctccc cgctgctttg
     8041 aggtggagga gaggttaaag aaggccctga cgatcccggt atttcatgac gatcagcatg
     8101 ggacggcagt ggtcgccctg gcagctctga tcaacgccct ccgcctggta ggaaaggact
     8161 ttcgggatct aaaggtggca gtgagcgggg caggggctgc aggggttgcc gtcaccaagt
     8221 tccttttaag cttcggagca aaggacgtca tactctgcga tagcagagga gtgattcata
     8281 aagctcgacc ggacctgaac ccctcaaagc aggagatcgc agctcttacc aatccacgca
     8341 atgtcaccgg cactctggcc gatgccattc gtggggcgga cgtctttttg ggcctctctg
     8401 tggcaggaat agtgacccca gacatggtga gcagcatggc cggggatgcc gtggtatttg
     8461 ccatggccaa tcccgagccg gagatcatgc ccgagctggc actggaggcg ggagcggttg
     8521 tggtggccac cggccgctcc gactatccca atcaggtgaa caatgtcctc ggctttcctg
     8581 gcatattccg gggtgctctg gacgtgcggg ccagcgatat caacgaacag atgaagatgg
     8641 ctgcctctcg ggctatagcc ggtctggtgg agaacgttac ccgggagtgc atcatcccct
     8701 ctcccctgga cagaagggtg gttcccgcgg tggcggaggc agtggcccgn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cctggtgcct gtcccgctgg tttgcccgcc
     8881 gcatggggcg cagaaagtag atcgaatgaa cttttttttg attagattga atcttgcgcc
     8941 ggccgccttt tcagctctct tctgccatca gatatcctgc cactgcccct gttacctctg
     9001 aggtagaaca tctgccgccc agatccggcg tcctcactcc cgcagccagc gcccttttca
     9061 ccgcctcctc tatcctgcct gccgcctcca gctcgcccag ccacctcatc atcatggcag
     9121 cgctgcggat cgctcctacc ggattggcaa tcccctttcc agcgatgtca ggagcgctgc
     9181 cgtgaatggg ctcgaacaga gcgcgagact tcccaagatt ggcgctggcg cacagcccca
     9241 ggcttcccac cacccccgcc gcctcgtcgc tcaggatgtc tccgaagagg ttggtggtga
     9301 ccaggacatc gaacctctga ggattagtca ccaggttgta ggcggccgcg tccaccagca
     9361 tatcctcata aagaatgccc cgccgctccg ccacctcccg gcaggtctgc aaaaagagcc
     9421 gatcgcttcg cagcacattg gacttatgga tgatagtaag acggcggcgt ctcgccgcca
     9481 ggtcgcatgc agcttctgcg atcctctccg aaccgcgacg ggttatcacc ctcagggtac
     9541 gcgattcctc cacccccacc tcctccactc cggagtagag cccctcggtg ttctctcgca
     9601 cgatgatgaa atcaaggtca tgggactgga agggccggat gttggcataa aggtccaggg
     9661 cccgccgcag ccttaagaga acgctccggt aattgggatc gggtggggtg gtgatcgcac
     9721 ccaaaagaat gcagtcgcag ccagaaatct cttccagatc atcttcgtcc atcgcttccc
     9781 ccgtcctctt ccagcggccc aggccgatct caaaatggac cagatcgagt gacgctcccg
     9841 ctgcccgcag cacctccagg gcgctttcaa ccacttccgg gcctatgccg tccccggcaa
     9901 tcactgctac cctcttagat ccttgaccag acaccttacc gcctccgcta tctcccaggc
     9961 accatctccc cagtggacaa caattcccgg ctggccgcac ttctccgtcc tcccccttcg
    10021 ctccgaccgg cagcagcgag tgatgaatgg cccctggggc tgaaattctc tttcccggca
    10081 atggatggga cagacattga tgaattcgtg ctcctttcct ggattgttgg ccagaaagat
    10141 ctctcgcgat acctcgcagc ccactatcct cggccgctcg tggaccacga ggtcgcagtc
    10201 caaagatcta cccagccccc ctgcccggca gggataatag acggacgggc agtagaacct
    10261 ctccaggttc aggatcctgg gcacgaacct caccatcagg tcccccagca ctccgcaatc
    10321 ctccagcccc tgcagggtgc agatcagcca gggcggatct ggtggggaga cgtccatgac
    10381 ctcgatcttc aggacctggc cggggtcggg
//
LOCUS       DAOSGP010000016        10264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5546_length_10264_cov_19.533941, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000016 DAOSGP010000000
VERSION     DAOSGP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5546_length_10264_cov_19.533941"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PLV49_01025"
     CDS             <1..1168
                     /locus_tag="PLV49_01025"
                     /inference="COORDINATES: protein motif:HMM:NF016765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nre family DNA repair protein"
                     /protein_id="HOG97453.1"
                     /translation="FVIKGKEAYLKKLTASMYIPSQEVGKELDGSTPPSVFIGSWNYP
                     KVLAGPMISPLHGDTMVMDHPESWIPENKTQEEIIRYRLNLIRGKRSVKVTDIENRFI
                     EKLRDVSLSSSSIESEAQFIQAPKGMSFDDVHTPFGPSAPLERFEIGNSRWDRDLEKV
                     YFDGDLKAADAVEDLHQRGLPFSSIQKAFSVGAMGIKKKRKLVPTRWSITACDSALGN
                     RLLQRVRDYDIIDCCQIREFASLNNYYAVLLLPTAWQYEWMEAFLRIMGNEELIFSDF
                     ETNGGKKGYSRVGGCYYSCKMAVLEALERERRQAGAIILREAYAGYVPLGVFNVRENV
                     RNAMHQKPLEFEDMRAALGYISTRLELPVKSFIRQSDLLRGELKSRQTTLSSFL"
     gene            1221..2096
                     /locus_tag="PLV49_01030"
     CDS             1221..2096
                     /locus_tag="PLV49_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97454.1"
                     /translation="MPFEATRIMSGDMPPELRERVDRIAEEFMAIVDRLVDERHLQKG
                     ITRIQLPCSMVFILEAQWLYILGQFRMENRITGYYNRPDKSLTLQKVASNARWEFGFD
                     DAFVIQLPADLLEKSTEERKSSLQEIASKHLDAEFLRLAQLLNLIRTRPIFGQATQPV
                     QPGSLLLLLAGDEAARMNADSILRAIGASGLAGDEAEDIRSGKSAVRDLWRSINQAAL
                     IIADLTGADPQVMYALGIAHTLGKDTILIHPQGSKYLIDIPKTQSIEYEDSIQGREKL
                     EEQISETLRSMTASL"
     gene            2353..3384
                     /locus_tag="PLV49_01035"
     CDS             2353..3384
                     /locus_tag="PLV49_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOG97455.1"
                     /translation="MHYDINGYRAGDIDVVAAFDIDARKVGKDVSQAIFAKPNCTNIF
                     YSDIPQKNVEVKMGPVFDGVAPHMENYPEERRFVVADAEPVDVTRELVDSGADVLVNY
                     MPVGSENATRFYAQAALDAGVGFVNCMPVFIASNPEWAKKFQVAGIPIVGDDIKSQIG
                     ATIVHRALTQLFKDRACVMDRTYQLNIGGNTDFLNMLNRERLKSKKISKTEAVQSILD
                     TPLCDDDIHIGPSDYVPWQKDNKVCFLRMEGRIFGDTPINLELRLSVEDSPNSGGSSI
                     DAIRICKLAKDRKIGGPLLGISAYTMKHPPVQFPDELAKEMVEQFIRGEVSEQEIARP
                     KQRQAATAR"
     gene            3425..3748
                     /locus_tag="PLV49_01040"
     CDS             3425..3748
                     /locus_tag="PLV49_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG97456.1"
                     /translation="MFYRLNEAPGVQMLPGLIRRTLVSGKALMICRFDLESGVQIPEH
                     SHPHDQAGYVVSGRIQITINGNSLELGPGDSYCAPPGVLHSARALEATVVVDTFSPPR
                     EDYLS"
     gene            3818..5005
                     /locus_tag="PLV49_01045"
     CDS             3818..5005
                     /locus_tag="PLV49_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG97457.1"
                     /translation="MVTLEKIAHLAEDCAFDSLGPGMNINPKKLSTLGEGTRHDVCAS
                     TYSPRESPLPGICHAFTQDGRCVSLFKTLYTNHCSHQCNYCINATNCSRKVQTFSYTP
                     DELARITLSLYRSNYIEGLFLSSGAGRDEDLIMERMLETVQKLRKQYDFPGYIHLKIL
                     PGSSKAHIQQAMELADRVSINLEAASASQLNEICATKSYENDILQRQRYIRDLRTDEN
                     LPAGQTTQLVVGAAGESDQDIFKRILYEYKDIGVKRAYYSAFSPQNGTAFESREGQPL
                     WREHRLYQMDWLYRIYHFPPADIHMAFDENGFLSNSDPKLAIARELLDSPVDPNSAAY
                     RELIRVPGIGPRSAKRIVGLRMRKNIVSKRELEALGVVIKRAAPFLKINGWRDTTLEM
                     WSG"
     gene            5002..5634
                     /locus_tag="PLV49_01050"
     CDS             5002..5634
                     /locus_tag="PLV49_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4130 domain-containing protein"
                     /protein_id="HOG97458.1"
                     /translation="MRVPDNYCRYLVMHAKSNERLLARTEGLTALDLEVSASPEAVSL
                     RRMVYAVMSEVHRMKAFIRLRPLGDLVLWGYMKPRHRIGEHTSEHFARRNPGTIIVLG
                     NGSESWTAFFRQGRMMGIHGAGLDETLDRLKSALKISEEEKGADAEGAKANDQDVDDI
                     WQTYYDSQYCPERKNLQAFRQRMPARDQEAAGLQLMQKKREMTLEDFWQQ"
     gene            5889..6200
                     /locus_tag="PLV49_01055"
     CDS             5889..6200
                     /locus_tag="PLV49_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97459.1"
                     /translation="MSALIGDLKKIPQEKGPEVELAVFGDEFYARYENKGGYAAIYDE
                     EIGLFTYAEVIDGKYVSTGVAITKEPPGNLKPHLRESDEVMREKAQRRFEKREPPSLS
                     G"
     gene            6269..8506
                     /locus_tag="PLV49_01060"
     CDS             6269..8506
                     /locus_tag="PLV49_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOG97460.1"
                     /translation="MSAIKGRVVEVPQENGPAVKLWMSGDEFYVRYENLEGYTVVYDG
                     KLGLFTYSVLVNGELVSSGVPISNPAPEGLKKHEREDPSVRQRKFELKYARMLPPITR
                     TMDERPRTIGEQHGLLRGRLLHEGKIKGLTILVEFPDEKINVSAANISEMLNKTGYNE
                     GGNFCSVRDYYLKMSNGKLDYTNEVIGPITLKKNKMYYHFNLFVEEAMDAVVGMGIDL
                     SRFDSKGVGLIDALSFLYAGNAVYDGELHPHNSYIDLKFGDIKTYLYMLSNLGTSKDD
                     LAIGTICHETGHLLCRFPDLYDYGRRDEDLVESAGLGLYCLMSVGDHLDDWKTPSPVC
                     AYFRNLAGWCDNVVDLNKPGKYEAKHGDYRTVMKYTTDRANEYFLVENRSQMGLDRAL
                     PSSGLAVYHCDILGSNEWQEGSPSKHFQCALLQRDGSLHLERGLNYGDGTDLYKKVNG
                     VALSYDTNPSSKIWDRSDSGFIISDISEPGEVISFVVGPVVSSDTVKGEANVSIAIPD
                     NDVHGVSSSLNISEEGRASRIYLSLDISHSYISDLKVELISPSGQRAMIHNREGGSEG
                     NLIKTYDSNSLPSLGDLVGSPINGEWSLTVADVAAIDKGVLNRWSIEIEKEASEMEVS
                     KDLEPNELIPDNDPRGASVAMNLDKKGIVQRVVTMVDITHPFIGDLRVEILSPSGKTA
                     IIHDRGGFDQDNLVKTYDSQINPELQAFVGQPAEGNWILRAMDLSRLDKGIINKWNMR
                     VTYSG"
     gene            complement(8592..>8865)
                     /locus_tag="PLV49_01065"
     CDS             complement(8592..>8865)
                     /locus_tag="PLV49_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG97461.1"
                     /translation="VQIPPEKGYGNYKEERAITMDKKDFPSDLVPVEGMSLEICTSEG
                     IYVPAQITDVTETTVTLDANHPLVEQTLFFDIMLLEITKSMEKSAQ"
     assembly_gap    8866..8965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9025..9903
                     /gene="htpX"
                     /locus_tag="PLV49_01070"
     CDS             9025..9903
                     /gene="htpX"
                     /locus_tag="PLV49_01070"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOG97462.1"
                     /translation="MRWKADRGLEARMLLTMILLAVIYLAFLAVLVSQGVDNIVIILF
                     IGGFMLLQYYYSDKMILSTMGAKMVSESEAPELHQIVSRLCAIADLPMPKIAVVNSSM
                     PNAFATGRNQKNAVVAVTTGIMQKLDHSELEAVLAHELTHVKNRDMMVMTIATFLSSM
                     AQILVRSMPFMGSGGGRGRDSGGSFIVVFVVSLAVWVLSFILIQALSRYREFAADRGA
                     AIITGQPSNLVSALMKISGFRVPTEDLRKVEGPVSALFIVPAFSRSSIMNLFSTHPTL
                     EARIEALQRIEQGLES"
     gene            9906..>10264
                     /locus_tag="PLV49_01075"
     CDS             9906..>10264
                     /locus_tag="PLV49_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97463.1"
                     /translation="MGFLDSIMGKTRLPEAKIDRLFAISTAAVTMDANLDLVPDGQAG
                     LCFKPMESSYYESASKEIEDLLKLSFQESGTSHSLQKDEYGYIWVILTDQDFEDLVAN
                     IQMICQILMEQGFGTQL"
BASE COUNT         2673 a         2345 c         2765 g         2381 t
ORIGIN      
        1 ctttgtgatc aagggcaagg aagcctatct aaagaagctg actgccagca tgtatatccc
       61 ttcccaagag gtaggaaagg agctggatgg cagcacccca ccttctgtct tcatcggcag
      121 ctggaattat cccaaagtac tcgccggacc tatgatttct cccctgcatg gagataccat
      181 ggtcatggac catcctgaat catggatacc ggagaataaa acccaggagg agatcataag
      241 gtaccggctg aacctgatac gtggcaagag atctgtcaag gtcactgata ttgagaatcg
      301 ctttatagaa aagcttcgcg acgtctccct atccagttcg tccattgaga gcgaggccca
      361 gttcattcag gcccccaaag gcatgtcctt tgacgatgta catacgccct ttggtcccag
      421 cgctcctctg gagaggtttg aaatcggcaa ctctaggtgg gatcgagatc tggagaaggt
      481 ttacttcgat ggggatctga aggcggcgga tgcggtggaa gatcttcacc agcgtggcct
      541 tcccttctcc agcatccaga aggccttctc agtgggtgca atgggcatta aaaagaaaag
      601 gaagctggtt cctacccgct ggtctatcac cgcctgtgac agcgcccttg gcaatcgtct
      661 tcttcaaagg gtcagggatt atgatatcat cgactgctgc cagataaggg agtttgccag
      721 cctcaacaac tactatgccg ttcttctcct ccccactgcc tggcagtatg agtggatgga
      781 ggcattcctg cgcatcatgg gaaacgagga gctgatattc tccgattttg agacaaatgg
      841 cggcaagaag ggatactccc gggtgggagg atgctattat tcctgcaaga tggcggtcct
      901 ggaggcgctg gagagggaga gaaggcaggc aggggcgata atactcaggg aagcctatgc
      961 cggttatgtg cccctggggg tctttaatgt tcgggagaat gtgagaaatg caatgcacca
     1021 gaagcccctg gagttcgagg acatgagggc tgctcttgga tacatatcca caaggcttga
     1081 gcttccagta aagagtttta tccgtcagag cgatcttctc cggggggagt taaagagccg
     1141 ccagaccaca ttgagctcct ttttatgata ttctcatttt gctggaaacc tactttaagt
     1201 cggtgattga attaccttct atgccattcg aagctaccag gattatgagc ggtgatatgc
     1261 ctccagagct cagggagaga gtggacagaa ttgcagagga gtttatggcc atcgtcgatc
     1321 gcctggtcga tgaacgccat ctccagaagg gaataaccag aattcagctg ccatgcagca
     1381 tggttttcat ccttgaagct cagtggcttt acatcctggg tcagttcaga atggagaaca
     1441 ggattacagg ctactacaat agaccagata aatcattgac cctccagaaa gtagccagca
     1501 acgctaggtg ggagtttggt tttgatgatg cttttgtgat ccagcttcca gctgatctgc
     1561 tggagaagag cacagaggaa aggaaaagct cattgcagga gatcgcctca aagcaccttg
     1621 atgcagagtt tctgaggctg gcccagcttt taaacttgat acgcacccgt cccatatttg
     1681 ggcaggccac ccaaccggta cagccaggaa gccttctatt gttgctagct ggggatgaag
     1741 cggcaagaat gaatgcagat tcgatcctca gggcgatcgg tgccagcggg ctggcaggcg
     1801 atgaggctga ggacatcaga agcggaaagt ctgccgtccg ggatttatgg cgctctatca
     1861 atcaagcggc gttgattatt gccgacctga cgggagcaga tccccaggtc atgtacgcct
     1921 taggcatcgc tcatactctg ggcaaggata ccatcctcat ccacccgcaa ggctcaaaat
     1981 acctgattga tattcctaaa acacaaagca ttgagtatga ggatagcatt cagggcaggg
     2041 aaaagctgga ggagcagata agcgaaactc tgcgttccat gacggcatcc ctataagatt
     2101 gataagactt gagagatcaa taggattccg ggacaggcca tattcaccga agcctataac
     2161 gttatatatg tcttgccttt atacgaagct ccgaatccta actcaggtct ccaggcttca
     2221 catgacccag gaggggaaaa gagtttgaag aagataaggt tggcaatcgc gggcatagga
     2281 aactgcgcaa gctccctagt tcagggaatt gaatattata aacatgttga taaagaaagt
     2341 tgtattgggc tgatgcatta tgatatcaac ggctatagag ctggtgacat tgatgttgtc
     2401 gctgcctttg acatagatgc tcgaaaggta gggaaggatg tgagccaggc tatatttgcc
     2461 aagcccaatt gcaccaatat cttctactct gatattcccc aaaaaaatgt tgaggtcaag
     2521 atgggacctg tttttgatgg cgttgcgcct cacatggaaa actatcctga ggagaggagg
     2581 ttcgttgtgg ctgatgccga gccggtggat gtaacccggg agctcgtcga cagcggtgcc
     2641 gacgtcctgg taaactacat gcccgtaggg tcggagaatg ccactcgctt ctatgctcag
     2701 gcggctttag atgccggggt gggatttgta aactgcatgc ccgtattcat cgcatctaat
     2761 cccgaatggg caaagaagtt ccaggtggca gggattccaa tagtgggaga tgacataaaa
     2821 tctcagatcg gcgccaccat tgtgcaccgt gctctaactc agctcttcaa ggaccgggcc
     2881 tgcgtcatgg acaggactta ccagcttaac atcggcggca acaccgactt tttaaatatg
     2941 ctcaatcgcg agaggctcaa gtccaagaag atatccaaga cggaagcggt ccagtccatc
     3001 ctggacacgc cactgtgcga tgatgacatt catataggac cctcggatta tgtgccatgg
     3061 cagaaggaca acaaggtctg cttcctgaga atggagggca ggatattcgg agatacgcca
     3121 ataaatctag agctgcgtct ctcagtcgag gactctccca atagcggagg aagctccatc
     3181 gatgccataa ggatctgcaa gctggcaaag gatagaaaga ttggagggcc actgctgggc
     3241 atatccgcct ataccatgaa gcacccccca gtgcaatttc ctgatgagct ggccaaagag
     3301 atggtggagc agttcatccg gggtgaggtc tctgagcagg agattgccag gccaaagcag
     3361 cgccaggcgg caactgctcg atgactggga ctttttgctt ttagcctatt ctgagatgaa
     3421 taaaatgttc tatcggttga atgaggctcc cggagtgcag atgttgccag gcctcataag
     3481 gcgcaccctt gtcagcggaa aggctttgat gatctgccgg ttcgatctgg aaagtggggt
     3541 ccagatccct gagcattctc atcctcacga ccaggcgggc tatgtagtct caggcagaat
     3601 tcagattaca ataaacggaa atagcctcga gctggggcca ggggacagct actgtgctcc
     3661 accaggagtg ttgcacagcg ccagggctct ggaggcgaca gtggttgtgg atactttcag
     3721 cccgcccaga gaggactatc tctcctaata gcctctttcg ctcactttcg cttcgcaagc
     3781 acatcggtta taatcgatga ggcccaatta tatttgaatg gtcaccctgg agaagatcgc
     3841 tcacctggct gaagattgcg cttttgactc cctcgggcca ggcatgaata tcaatccgaa
     3901 gaaactctca accctaggag agggaacccg gcatgatgtg tgcgcatcca cctattcccc
     3961 tcgcgaatcg cccctgcccg gaatctgtca tgccttcacc caggatggca ggtgtgtgag
     4021 cctcttcaag accctctaca ccaaccactg cagccatcag tgcaactact gcatcaatgc
     4081 caccaattgc agccgaaagg tgcaaacctt ctcctatact ccagatgagc tggccaggat
     4141 caccctctcc ttatatcgct ccaactatat cgaaggactc tttctcagtt cgggcgctgg
     4201 cagggatgag gatctgatca tggagaggat gctggagact gtgcagaagt tgcgcaagca
     4261 atacgacttt ccgggataca tccatctcaa gattctgccc ggctcatcca aggcccatat
     4321 tcagcaggct atggagctgg cagaccgggt gagcataaac ctggaagctg ccagtgcctc
     4381 gcagctgaat gagatctgtg ccaccaagag ctatgagaat gatattctgc aaaggcaaag
     4441 atacattcgc gacctgagaa cggatgagaa tctgcccgca ggccagacca ctcaattggt
     4501 ggtgggggct gcgggagaga gcgatcagga catctttaaa agaattctat atgagtataa
     4561 agatattgga gtaaagcgag cttactatag cgctttttct ccccagaatg gaacagcatt
     4621 tgagtctcga gaaggccagc ccctctggcg ggagcaccgc ctctatcaga tggactggct
     4681 ctaccggatc taccatttcc ctccggctga cattcacatg gcatttgatg agaacggctt
     4741 tctatccaac tccgatccca agctggccat agccagggag ctattggatt cgcctgtgga
     4801 tcctaactcg gccgcctacc gggagcttat ccgggtgcca ggcatcgggc cacggagcgc
     4861 gaagagaata gtcggcctga gaatgaggaa gaatatcgtc tcgaaaaggg agcttgaggc
     4921 tctgggggtc gtgatcaaga gagcagctcc cttcttgaag atcaacggct ggagggatac
     4981 caccctggag atgtggtcag gatgagggtg ccggataact attgccgcta cctggtcatg
     5041 cacgctaaat cgaacgaaag gcttcttgcc aggacggagg gcttaaccgc tctggatctg
     5101 gaggtctcgg ccagtcctga ggctgtgagc ctgagaagga tggtctatgc cgtgatgagt
     5161 gaggtgcaca ggatgaaggc attcatcagg ctcaggccat tgggagacct ggtcctctgg
     5221 ggctatatga agcccaggca caggatcggg gagcacacga gcgagcactt cgcccgccgg
     5281 aatcctggca cgataatagt cctgggaaat ggcagcgaga gctggacggc attcttccgg
     5341 cagggcagga tgatgggaat tcatggggcg ggattggatg agactctgga tagattgaaa
     5401 tctgccctga agatatcaga agaagaaaaa ggggcggatg ctgagggtgc caaggcaaat
     5461 gatcaggatg ttgatgatat ctggcagacc tattatgaca gccagtactg cccggaaaga
     5521 aagaacctgc aagcatttcg ccagagaatg ccagcgcggg atcaggaggc tgccgggctg
     5581 cagttgatgc agaagaaaag ggagatgacc ctggaggatt tctggcagca atagtcggtt
     5641 ctctaaattg gagaggtttg ctcttctcca ttaacttggg ctaatcgctt gtttaatgga
     5701 cgaaagttta ataggtgcga atggcatagt acttatatct ttaactggca gaatattctg
     5761 ctattaaaaa tccagaatcc agagggctta ctacgagaga cgaaagcaag aaatatcgtc
     5821 aatattttcg caggagaatg ggataatctc ctcaatctga atgagcctaa tgatgcgagg
     5881 cgatttagat gtctgcatta attggagatc tgaaaaaaat accacaagaa aagggacccg
     5941 aagttgaact agccgtcttc ggggatgagt tttatgctcg ctatgaaaac aaaggtggtt
     6001 atgcagcgat ctacgatgag gagattggtc tgttcaccta tgccgaggtt atcgatggca
     6061 aatatgtctc cacaggagtt gccattacaa aagagcctcc tggaaatctc aagcctcatc
     6121 tgagagagtc agatgaggtt atgagagaga aagcgcaaag acggtttgag aaaagagaac
     6181 caccgagctt gagcggatag ctcttgaagc tgaaaatatg agcgaaattt tctttttttt
     6241 aatcgatcaa atatggagat gagagcacat gagtgcaatt aaaggaaggg tcgtcgaggt
     6301 tcctcaagag aatgggcctg ctgtaaagct ttggatgtca ggagatgaat tctatgtcag
     6361 atacgagaat cttgaaggtt atacggtggt ctatgatggc aagctgggcc tgttcaccta
     6421 cagcgttctc gtcaatggag aactggtatc aagtggagtg cctatttcaa atcccgcccc
     6481 agaaggatta aaaaagcacg agagagagga tccatccgtt cgtcagagaa aattcgaact
     6541 caaatacgcc agaatgctgc caccaattac ccgaaccatg gacgagcggc cacgaactat
     6601 aggcgagcag catggactgc tgagaggcag gctccttcat gaaggaaaga taaagggtct
     6661 cacaatcctg gtagagttcc ccgatgaaaa gatcaatgtc tctgcagcca atatatcaga
     6721 aatgctcaat aagacaggat acaatgaggg tggaaacttc tgctctgtcc gggattatta
     6781 ccttaagatg tccaatggaa agctcgacta tacaaacgaa gtgatagggc cgataacact
     6841 gaaaaaaaac aaaatgtatt atcacttcaa cctctttgtt gaggaggcca tggatgcagt
     6901 cgtgggcatg ggtatagacc tatcccgatt cgactccaag ggagtcggct taatcgatgc
     6961 tttgagcttc ctttatgcag gaaacgccgt ttacgatggc gagcttcatc ctcataactc
     7021 gtacatcgat ctgaaattcg gcgatatcaa gacctacttg tacatgctct ccaatctggg
     7081 aacgagcaaa gacgatctgg caataggcac catctgccat gaaacaggac acctgctatg
     7141 tcgcttcccg gatctctatg attacgggag aagggatgaa gatcttgttg agagcgcagg
     7201 acttggttta tattgcctga tgagcgttgg agatcatctg gacgattgga agacgccatc
     7261 tcctgtttgt gcctatttta gaaacctggc aggatggtgt gacaatgtgg tggatctcaa
     7321 taagccaggg aagtatgaag ccaagcatgg cgattatcga accgtgatga aatacacgac
     7381 tgacagggca aatgagtact tcctggtgga aaaccgctct caaatgggtc tggacagggc
     7441 tcttccctcc agtggattgg cggtctatca ctgcgacatt cttgggtcca atgagtggca
     7501 ggagggttcg ccatccaagc attttcaatg tgctctgctt cagagagatg gcagtctgca
     7561 tcttgaaagg ggactcaact atggtgatgg aacggatcta tacaagaagg tcaatggcgt
     7621 tgccctatcg tatgatacca atccatcttc aaagatatgg gatcgctccg attcaggttt
     7681 tataatttca gatatcagtg aaccaggaga ggtaatatcc tttgttgtgg ggccggtggt
     7741 ctcttccgac acggttaaag gggaggcaaa tgtatccatt gccatacctg acaatgatgt
     7801 tcatggagtg agcagctcat tgaatatctc tgaagaagga agggcgtcca ggatctattt
     7861 aagcctcgat atatctcatt cctatatcag cgacttgaag gtggagctca tctcgccatc
     7921 cggtcagaga gcgatgattc ataatcgcga ggggggaagt gaaggcaatc tgataaagac
     7981 atatgactcc aattcgctgc cttctttagg ggatctggta ggttcgccaa tcaatggcga
     8041 gtggtcactg accgtagcag atgtagcagc cattgacaag ggcgtgctca atagatggtc
     8101 aattgagatt gagaaagaag cctctgaaat ggaagtgagc aaagatctgg agccgaatga
     8161 gctaatacca gataatgatc cgagaggggc tagtgtggcg atgaatcttg ataagaaggg
     8221 aatcgtccag agagttgtga ccatggtgga tataactcat cccttcattg gagatttgag
     8281 ggtggagata ctgtctcctt ctggaaagac tgccattatc catgatagag gaggcttcga
     8341 tcaggataac ctggtcaaga cctatgactc gcagataaat ccagaattgc aggctttcgt
     8401 aggccaacca gcggaaggaa attggatact cagggctatg gatctgtcaa ggctggataa
     8461 aggcattata aacaaatgga atatgagggt cacatattct ggatagatac gcttttttcc
     8521 gggaaaatgg ggcataatcc tgcccctccc cccaaaatct atggctcttc gctgaatggc
     8581 caaatcttcc ctcattgagc agacttttcc atgctcttcg ttatctctaa tagcataata
     8641 tcgaaaaaaa gcgtctgctc gacgagggga tgatttgcat ccagagtgac tgtggtctct
     8701 gtaacatctg taatctgcgc agggacataa ataccctcgg aggtgcagat ctcaaggctc
     8761 attccctcga cgggaacgag gtctgaaggg aaatcctttt tatccatggt tatagcccgc
     8821 tcttccttgt aattgccata gcccttttcc ggagggatct ggaccnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnagtag cgacaaatgc tggctgatgc aatcaaggta
     9001 tatcaagggt gaattcaaga tatgatgaga tggaaggcag atcgagggct tgaagccaga
     9061 atgcttctga ccatgatcct tttggcggtc atctacctgg cgtttctggc agttctggtc
     9121 tcccagggcg tcgataatat agtaattatt ttattcattg gcgggttcat gctccttcaa
     9181 tactactatt ccgataagat gatcctgtcg accatgggcg ccaagatggt ctcggagagt
     9241 gaagctccag agcttcatca aatcgtctcc cggctctgcg ccatcgccga tttgcccatg
     9301 cccaaaatag cagtggtaaa cagctcaatg ccaaatgcct tcgctaccgg caggaaccag
     9361 aagaatgcgg tggtggcggt gaccacgggc attatgcaaa agctcgatca cagcgagctg
     9421 gaggcggtgc tggcccatga attgactcat gttaagaaca gggatatgat ggtgatgacc
     9481 atagccacgt tcctctcctc catggcccag atcctagtca gatccatgcc gttcatgggc
     9541 agtggaggcg ggcgcggtcg cgactcagga ggcagcttca tagtggtatt cgtggtctcc
     9601 ctggcggtgt gggtcttgag cttcatactg atacaagcac tctccagata ccgggagttt
     9661 gccgccgacc gcggagctgc aatcatcacc gggcagccct ccaacctggt gagtgcattg
     9721 atgaagataa gcggctttcg ggtgcccacc gaggatctga gaaaggtgga ggggcccgta
     9781 agcgctctgt tcatagtgcc tgccttcagc cgctcttcca tcatgaacct cttctcgacc
     9841 caccccactc tggaggccag gatcgaagct ctgcagagga ttgagcaggg actggagagc
     9901 tgaagatggg atttctggat tccattatgg gaaagacgcg cctgcccgag gccaagatcg
     9961 accggctatt tgccatctcc accgctgcgg tgacaatgga tgccaacctc gatcttgtgc
    10021 cggatgggca ggctgggtta tgcttcaagc ccatggagtc ctcttactat gaatctgcca
    10081 gtaaggagat tgaagacctc ctcaagctga gcttccagga gtcgggcaca tcgcacagcc
    10141 tccagaagga tgagtacggc tatatctggg tgatcctgac cgaccaggat tttgaggatc
    10201 tggtggccaa tattcagatg atctgccaga tattgatgga gcagggattc ggaactcagc
    10261 tctt
//
LOCUS       DAOSGP010000017        10146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5639_length_10146_cov_19.280943, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000017 DAOSGP010000000
VERSION     DAOSGP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5639_length_10146_cov_19.280943"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..87)
                     /locus_tag="PLV49_01080"
     tRNA            complement(13..87)
                     /locus_tag="PLV49_01080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Arg,seq:cct)
     gene            complement(171..>1292)
                     /locus_tag="PLV49_01085"
     CDS             complement(171..>1292)
                     /locus_tag="PLV49_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOG97464.1"
                     /translation="GGAGFAGVTFEGQADLRNTDFGGQASFLSAQFSEDASFRNSQFA
                     GDGIFLNSSFARDVDFDFAQFQRLASFADASFVNVSFLETQFGGHTSFLNAKFHGNAA
                     FAATRFAGSVVFREAGFLLGSTFGLSSFGGLADFTNVYFNKTAYFGGGKFTDLAYFVN
                     ARFDGDLNMEDSRLYNMRLDNVSLQEDSKINLNNADFTKLEVRWAVIKDRLVYNGAAY
                     LALVKNYKSLEWFDDADDCYYQYRRIGQDQAPWGWGKISDLISWLSCGYGVRVSYTAF
                     WCLFTILFFGVIFWAGKGMNKFEIEGMELPGDHRLRPTARVSFTDALYFSIAMFTTSQ
                     APVNTYPVGFYRHLAMAEGILGWFFLGLFVVVLSGVLIR"
     assembly_gap    1293..1392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1461..2387
                     /gene="twy1"
                     /locus_tag="PLV49_01090"
     CDS             1461..2387
                     /gene="twy1"
                     /locus_tag="PLV49_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HOG97465.1"
                     /translation="MQDTMAEKTLARQGYHLVGSGAVKPCLWLNRSMRGGDQCYKNHF
                     YGIASHRCVQMTPTLECNHLCLHCWRPIDDPIPGKEPMEPAELLEGILRGQQRFISGY
                     GGSSTTDPVRLVEAREPKHMAISLMGEPTLYPYLKEFIDLVSRRGMTSFLVSNATRPE
                     VLAELRPTQLYLSLNAPDEERYRRICNPTKDLWPRILESLELLKEHRCRSVIRMTLVR
                     GQNMVGLEDYARLIGDAEPDFVELKAYMHLGRSRTRLERTAMPQHSEILALADSLAGL
                     LGYHLEADVPLSRVALLSRGRISRFIEMRDHK"
     gene            2581..2973
                     /locus_tag="PLV49_01095"
     CDS             2581..2973
                     /locus_tag="PLV49_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG97466.1"
                     /translation="MDREIKIILVEDNAEDIAFTRRVLKHNELNGNLIVATNGKEALE
                     ALQSMNGSDLPELILLDINLPDISGIDLLTLIKKEQRLKDIPVVILTGSNEDQDIQKC
                     YDLGASSYLVKPISNDALMLVVEKLFDQ"
     gene            complement(2959..3813)
                     /locus_tag="PLV49_01100"
     CDS             complement(2959..3813)
                     /locus_tag="PLV49_01100"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOG97467.1"
                     /translation="MLRLGMHVSIAGGLEKAADRAREKGCDAFQIFTTNPRSWRSKPI
                     AQQSIDFFSFRLKEYDLHPVVSHMPYLPNLASPRDEVYSRSVQALTDELVRCRLLGIP
                     YLVTHLGSNLGAEKKDGRERIAEALKKAIADSGGDVMVLLENGAGTKNSMGSTFKDIA
                     SIIESLPGQSRSLGVCLDTCHLFAAGYDLRTPRALERTLEEFDCCISSNRLKLVHLND
                     CQGTLGSHLDRHEHIGLGQIGLEGFRSIVCHPSLRELPMIMETPVDSRRDDKANLLVV
                     RALASLIE"
     gene            complement(4001..4309)
                     /locus_tag="PLV49_01105"
     CDS             complement(4001..4309)
                     /locus_tag="PLV49_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97468.1"
                     /translation="MYSDILTICWSIKEVNRNLSDRQATSDYSIRYLKKGCSDLALMM
                     RELGRALPDDKIEVIDRNGQKKSFSINEVSDMLYDTKKILEFNLIDNISRWAEARKLA
                     "
     gene            complement(4377..4579)
                     /locus_tag="PLV49_01110"
     tRNA            complement(join(4377..4412,4542..4579))
                     /locus_tag="PLV49_01110"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4543..4545),aa:Trp,seq:cca)
     gene            complement(4590..4663)
                     /locus_tag="PLV49_01115"
     tRNA            complement(4590..4663)
                     /locus_tag="PLV49_01115"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4627..4629),aa:Lys,seq:ctt)
     gene            complement(4837..>6184)
                     /gene="glnA"
                     /locus_tag="PLV49_01120"
     CDS             complement(4837..>6184)
                     /gene="glnA"
                     /locus_tag="PLV49_01120"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOG97469.1"
                     /translation="RAGDDNRMYSQEQMLKIIQEKNVEFLRLQFTDISGIVKNVAIPA
                     TQMGKALKSGISFDGSSIEGFARIQESDMVLRPDLSTFSLLPWSTKDDVNEARLICDV
                     HLPNGLPFEGAPRRVLYRQLERAKEMGFKMNVGPELEFFLFEKGNGGSATTPHDFGGY
                     FDLGPVDLAEDVRREITRALIKMGFTIEASHHEVARGQHEIDFVYDDAIKNADKVVTF
                     KYVTKTIAMRQGLRATFMPKPVYGAAGSGMHVNISLFRGLENAFYDPETPLNISDLAR
                     FFVGGLIEHASAITAIANPLINSYKRLVSGFEAPVYITWSGPNRSSLIRIPSGRGLST
                     RLEFRSPDTSCNPYLTFAVVLAAGLDGIKRGIDPGDPVDLNVYHLTAAERKSLGIQTL
                     PSNLKEALDHLEGDKVIREALGEHVYENVMRLGMLEWEEYNKFVHPWEIDRYINTY"
     assembly_gap    6185..6284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6287..7714
                     /gene="purD"
                     /locus_tag="PLV49_01125"
     CDS             6287..7714
                     /gene="purD"
                     /locus_tag="PLV49_01125"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOG97470.1"
                     /translation="MNLMSGNRVLVVDAGGRGNAIAHAFARSGTVERVYVAPGNAGSE
                     MMDKCRLAAIEGKVPRSISELLAFVKKEKIDLTFVGPEGYLSEGIVDIFQEDGQRILG
                     PKREAAVLENSKCQTKDLLKKLRVPVPPCQSFSDPQEAKEYVQQRCSEEPGRGLVVKA
                     DGLAAGKGSIVCATPEEAASAIERIMVSPRLFGEAGDRIEIEDRLEGRELMFFALSDG
                     HTVQAMQSALDYKQAFSPDESVAIRLFNKLAGNPNQDNNPNTGGMGGFSPHPWLDEEL
                     TERIMKKIALPTVRGFKESSGQEYRGVIYFGLMISEDREPAVLEINVRLGDPEAEVIL
                     PRLQTDFYQLSMAVVDGRLDELPLQWSDDFCLGICAISGRAIKPLSSTGPERPGYPGE
                     HYTNMPINGLERVDPEVIIYHNGTAWGTDSAGNRRLYTTGGRVFTLVARGKSLAEARA
                     KAYENIKRVRFNGMRYRKDIGLRYI"
     gene            7715..8713
                     /locus_tag="PLV49_01130"
     CDS             7715..8713
                     /locus_tag="PLV49_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HOG97471.1"
                     /translation="MDRGARSRMIIFGLEGTAWNLSAALVDESGVIYEKSATYTPARG
                     GIHPREASQHHAEHMRAVVGDVLAQARQRGLKLEGVAFSQGPGLGPCLRTVATAARAL
                     SLRFDIPLVGVNHCVAHIEVGKWQSGARDPAVIYVSGANSQVLALRQGRYRIFGETLD
                     ISVGNAIDKFARSVGLAHPGGPKVEELARKAKNYIPLPYTVKGMDLSFSGLSTAATEA
                     AGKHDLEDVCYSLQETAFAMLVEVTERAMAHAEKREAMLVGGVGANARLGEMMRIMCH
                     ERGAEFFLPPRSFMGDNGSMIAYTGLLMLKSGISTPLDQSHVRPGYRTDEVPVSWA"
     gene            complement(8723..9418)
                     /locus_tag="PLV49_01135"
     CDS             complement(8723..9418)
                     /locus_tag="PLV49_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97472.1"
                     /translation="MNEVSRGILYPALAVLLAGLISVGLVDAVQADQMEGLDGGNDQM
                     SPERRGGEDLYRNPDIPMGLPVDLLFSGHGFAIRGNESHPIRLKVEVLLPVQPEQIRE
                     LVASNKSLKEIRDDIRENVGDEVHRGSLIFDRRLYAAINVSSLPGSNNSTLLEADLAD
                     MNSLSAGNDSAILGRINLTISSCIEGTTGRGDLEIDRPPHEGRYFVLLDMEPPKEENW
                     RSRSQAEGPEPGK"
     gene            complement(9546..10136)
                     /locus_tag="PLV49_01140"
     CDS             complement(9546..10136)
                     /locus_tag="PLV49_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97473.1"
                     /translation="MNELNWRMALIALAASCLLISGALSMPMDRGMGMHARGMMISAP
                     LNVTSEELGNMTLGEIKELEMSALNSSLEEEMPFCGRMNARGGEWMDFRGASPAGFCG
                     FGILGCGFTPISLMDDVTSEELGEMSLNQILDLYDQKTNELENMTLNEIRDQWKTKVE
                     AQNNMTINELVDEAKDMQAKEGIINWVSIQYRRLVA"
BASE COUNT         2302 a         2769 c         2760 g         2115 t
ORIGIN      
        1 agaaaaagca agcgggcgca gagggattcg aacccccgat ctacagctta ggaggctgcc
       61 gccttatcct gactaggcca cgcgcccttc ttttggactc tcctggttta accaacagat
      121 atatcaacct ttccggatcg gcggctcaga ttttgctcac caattccatt ttagcggatg
      181 agaacgccgc tcaatactac tacgaacaga ccgaggaaga accatccaag gattccctct
      241 gccatggcca ggtggcggta gaagcccaca ggataagtgt tgaccggtgc ctgggaggtg
      301 gtgaacatgg cgatggaaaa gtaaagggca tcggtgaaag ataccctcgc tgtgggtctc
      361 aggcggtggt ctccaggaag ctccatcccc tcgatctcga acttgttcat cccctttccc
      421 gcccagaaga tcaccccgaa gaacagaatg gtaaagaggc accaaaaggc ggtatagctc
      481 acccgaacac catagccgca ggagagccag gagatcagat cagagatctt tccccagccc
      541 cagggtgcct ggtcctgccc tattctgcgg tactgatagt agcagtcgtc ggcatcgtca
      601 aaccactcca ggctcttgta gttcttgacc agggccagat aagcggcacc attgtaaacc
      661 agccgatcct ttatcaccgc ccagcgaacc tccagcttgg tgaaatcggc gttgttgagg
      721 ttgatctttg agtcttcctg aaggctcacg ttgtccagac gcatattata gagcctggaa
      781 tcctccatgt tcaggtcgcc atcaaaccgg gcattgacga aataagccag gtcggtgaac
      841 ttgccccccc cgaagtaggc tgttttgttg aaatagacat tggtaaaatc tgccagcccc
      901 ccgaagctgg agaggccaaa ggtgctcccc aagaggaatc cggcctcgcg gaagaccaca
      961 ctgccagcaa atctggtggc ggcaaaggcc gcattgccat gaaacttggc attcagaaat
     1021 gatgtgtggc cgccgaattg ggtctccaga aagctcacat tgacaaaact ggcatctgca
     1081 aaagaggcca gccgctggaa ctgagcgaaa tcgaagtcca catcccgggc aaagctgctg
     1141 ttgaggaata tgccgtctcc agcaaactgg ctgttcctaa agctggcgtc ttcagagaat
     1201 tgagcgctca gaaatgaggc ctgcccgccg aaatcggtgt tgcgaagatc tgcctgaccc
     1261 tcgaaggtga cccctgcaaa tccggccccc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nngggatatt tcgaccactg cgcaatagtt gagccgtcag ggatttatga
     1441 ctttgtattg gagggatatc atgcaagaca caatggcaga gaagaccctt gcccgccagg
     1501 gctatcacct tgtgggttct ggcgcggtca agccctgcct ctggttgaat cgctccatgc
     1561 ggggtggcga tcagtgctat aaaaaccact tttacggcat agccagccac cgctgtgtgc
     1621 agatgacacc aacacttgag tgcaatcacc tctgcctgca ctgctggagg ccgatagatg
     1681 acccaatacc aggaaaggag cccatggagc cggctgagct cctggaaggg atactgcgag
     1741 gccagcagag gtttatttcc ggctacggcg gatcttcaac caccgatcca gtgaggctgg
     1801 tggaggctcg cgagcccaag catatggcta tatccctgat gggagagccc accctctacc
     1861 cttatctgaa agagttcatc gatctggtct caaggcgggg catgacatca tttctggtca
     1921 gcaatgccac ccgtcccgag gttttggcgg agctaagacc aacccagctc tacctcagcc
     1981 tcaatgcccc agatgaagag aggtatcgcc ggatatgcaa tcccacaaag gacctctggc
     2041 cgaggatcct ggagtccctc gagctcctga aggagcaccg atgccgcagc gttatccgca
     2101 tgaccctggt tcgaggccaa aacatggtgg ggcttgagga ttatgcccgg ctgatcggag
     2161 atgctgagcc ggatttcgtc gagctgaagg cctatatgca ccttggccgc tcccgaaccc
     2221 ggctggagag gacggccatg ccccagcact cagagatcct ggccctggcg gattccttgg
     2281 ccgggctctt gggctaccat ctcgaggctg atgtgcccct gagcagagtg gctcttctct
     2341 cccgcggaag gatctccagg ttcatagaga tgagggacca taagtaggca ggagattttc
     2401 ccgcgcgggt gttggatagt ggttcatcat atttttcact gtggacttgc caatagcttg
     2461 cctggcaacc ctggctctgc ccttcagtgg gataactatt tttgcaagta gtttcaatta
     2521 tggacccgag gtttaaatcg aatatagggt gcatggtcat gggtcagcat gggtgagtta
     2581 atggatcgcg aaattaagat catactggta gaggataatg ccgaggacat cgcatttacc
     2641 aggcgggtct tgaagcacaa tgagctaaat ggaaacctta tcgttgccac caatggaaag
     2701 gaggcactag aagccctgca gagcatgaac ggaagcgatc tgcccgagct catcctgctg
     2761 gacataaacc tgcccgatat cagcggcatc gaccttctca cccttatcaa gaaggagcag
     2821 aggctcaaag atatacctgt ggtaattctc accggctcga acgaggatca ggatatacag
     2881 aaatgctatg acctgggggc gagcagctat ctggtcaagc ccatatccaa tgatgccctg
     2941 atgctcgttg tagagaagct attcgatcag tgacgcaaga gctctgacca ctagaaggtt
     3001 tgctttatcg tcccgtcgcg agtccaccgg cgtttccatt atcatcggca gctctcgcag
     3061 agaggggtgg cagacgatcg accggaagcc ttccaggccg atctgtccca ggcctatgtg
     3121 ctcatgtcgg tccaggtgcg aaccaagggt gccctggcag tcgttcagat gtaccagctt
     3181 gagccgattt gagcttatgc agcaatcgaa ctcctccaaa gtcctctcca gggcgcgggg
     3241 ggtgcgaaga tcgtatccag cagcaaaaag gtggcaagtg tccaggcaca ccccaaggct
     3301 gcgggactgg ccgggaaggg actcgatgat ggaggcaata tccttaaagg tgcttcccat
     3361 gctatttttt gtcccggcgc cgttctccag gagcaccatc acatcgcccc cggaatcagc
     3421 gatggccttc ttcaaggcct cagctatcct ctccctgcca tctttctttt ctgcgcccag
     3481 attgcttccc aggtgggtca ccagatatgg tatacccaga agtcggcagc gcaccagctc
     3541 atcggtcaga gcctgcaccg acctcgagta gacctcatct cgaggggagg cgaggttagg
     3601 aaggtagggc atatgagata cgactggatg cagatcatac tctttgagcc ggaaggagaa
     3661 gaaatctatg gattgttgag caatgggctt ggacctccag ctgcgaggat tggtggtgaa
     3721 gatctgaaag gcatcgcagc ccttctcccg tgctctgtcc gccgcctttt ccaggccgcc
     3781 ggcaatggag acatgcatac ccagtcgaag cataaactta gcagggatag agcaactgat
     3841 caaccttgcc caagggaaaa attcattata gaaatggata aaagataaaa cctggaccgc
     3901 aacgccgatg atgatcgagc tgttctgagt ggtgatgagc cctatgagtc ctgaggcgtg
     3961 cgattatctg atcgaggaac ttccgctttc tagatccagt tcatgcgagc ttgcgcgcct
     4021 cggcccaacg gctgatgttg tcaataaggt tgaactccag gatcttcttc gtatcataaa
     4081 gcatatccga cacttcgttg atggagaatg acttcttctg gccgttcctg tcgatcacct
     4141 cgatcttatc atccggaaga gcccgtccca actctcgcat catgagtgcc aggtcagagc
     4201 atcctttttt aagatacctt atggagtaat ccgatgtggc ttgcctgtcg ctgaggtttc
     4261 tattgacctc ttttatggac cagcaaatgg tgagaatatc actgtacatc tcggcacctc
     4321 ccagtcagtt catatcatag atatttaaac gctgtggaaa ggaccaggga aaaatgtggg
     4381 gctggtgaga ttcgaactca cgatcgacgg gtatctccga tactgaagtt cttacagcga
     4441 tttcaccgcc gtaacaaccc atttcagtgc tccaacggat catcaacgac ctccccgtcg
     4501 agagatctca gcagacccgt tgctcatcat taaatatacc gctggagccc atcgccctac
     4561 ctggctaggc cacagccccg gttttaaagc gggccgagag ggatttgaac ccccgaccta
     4621 aaggttaaga gcctttcgct ctgcctgact aagctaccgg cccggacccc ataatgatga
     4681 tgctaaatat atacgtttcg ggtgaagcag tgacatgagg ggctctgtgc cagatctgca
     4741 taatgctttt tcaggaaaag ctgctgaagg cttctggcag ggccatataa aaggccagaa
     4801 aaatgagcgg gcagaatccc tgcccttgaa attcactcaa taggtattga tgtatctgtc
     4861 gatctcccag ggatggacga atttgttata ctcctcccat tccagcatgc ccaatcgcat
     4921 gacgttctca tagacatgct cgcccagggc ctcgcgtatc actttatccc cctccaggtg
     4981 gtcaagagcc tccttcaggt tggagggcag tgtctggatt cccagggatt ttcgttctgc
     5041 tgctgtgaga tggtacacat tcaaatccac gggatcgccg ggatcaatgc ctctttttat
     5101 tccgtcaagg cctgctgcca ggacgacggc gaatgtcaga tagggattgc agctggtatc
     5161 aggggatcgg aactccaggc gggtggatag gccccggcct gaggggatgc gaataagcga
     5221 ggagcggttc ggcccggacc aggttatata gacgggagcc tcgaacccgg ataccagccg
     5281 cttataggag ttgatcaggg gattggcgat ggcggtaatg gcgctggcat gctctatcag
     5341 gccgcccacg aagaacctgg ccagatcgct gatgttcaac ggggtctcgg gatcataaaa
     5401 ggcattctcc aggccccgga atagagagat gttgacatgc attccgctgc ctgcagcgcc
     5461 gtaaaccggc ttgggcatga atgtcgccct caatccctgg cgcatggcaa tggtcttggt
     5521 gacgtacttg aatgtgacca ccttatcggc attcttgatc gcatcgtcat agacgaagtc
     5581 tatctcatgc tggccccggg caacctcgtg atgagaggcc tcgatggtga agcccatctt
     5641 gatcagagcc ctggttatct cccggcgcac atcctctgcc aggtccaccg ggccgaggtc
     5701 gaagtagccc ccaaagtcgt gtggagtggt agcagagccg ccatttccct tttcaaatag
     5761 gaagaactcc agctccgggc cgacgttcat cttgaatccc atctccttcg cccgctccag
     5821 ctgacggtac agcacccggc gaggagcacc ctcgaaaggc agtccattgg gaagatggac
     5881 atcgcagatc aatcgagcct cattgacatc gtctttggtg ctccagggca agaggctgaa
     5941 tgtggagaga tcgggccgga gaaccatatc cgactcctga attctggcga agccctcgat
     6001 ggatgaccca tcaaaggaga tgccgctctt caaagccttt cccatctggg tggcgggaat
     6061 ggcgacattc ttcactattc ccgatatatc tgtgaactgc aaccggagga actcaacgtt
     6121 cttctcctga atgatcttaa gcatctgctc ttgggaatac attcggttat catctcctgc
     6181 gcgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaatga atctgatgtc
     6301 cggcaataga gttctggtag tggatgcggg aggcagaggg aatgccatag cccatgcctt
     6361 tgcccggagt gggacggtgg agagggtcta tgtggcccca ggcaatgcgg gcagcgagat
     6421 gatggataag tgccgcctgg cggcgatcga gggtaaagtg cccaggagca tcagcgagct
     6481 gctcgctttt gtgaaaaaag agaaaatcga tctcaccttc gtgggtccag aaggctatct
     6541 atccgaggga atcgtcgata tatttcagga ggacggccag aggatcctgg ggccgaagag
     6601 agaggcagca gtgctcgaga acagcaaatg ccagaccaag gatctcctca aaaagctccg
     6661 tgtcccggtc ccaccctgcc agagcttcag cgatccccag gaggcaaaag agtacgttca
     6721 gcagcgctgc tccgaggagc cgggaagagg attggtggtc aaggcggacg gcctggcggc
     6781 gggaaaggga tccatcgtct gcgctacccc ggaggaggcc gcctccgcca tcgagcgcat
     6841 catggtaagc cccaggctct ttggcgaggc aggagatcgg atagagatcg aagatcggct
     6901 ggagggcaga gagctgatgt tcttcgccct gagcgacggc cacaccgtcc aggcgatgca
     6961 atcagcactg gactacaagc aggccttctc tccggatgag agtgtggcca tccgcctctt
     7021 caacaagctg gcaggtaatc ccaaccagga caacaaccca aacaccggtg gcatgggagg
     7081 cttctcccct cacccctggc tggacgagga gctgacggag aggataatga aaaagatcgc
     7141 tctccccacg gtgcgcggct tcaaagagag ctccggccag gagtacaggg gagtgatcta
     7201 cttcggcctg atgatctctg aggacaggga gccagcagtc ctcgagatca atgtgaggct
     7261 gggagaccca gaggcagagg tgatacttcc caggctccag acggacttct accagctttc
     7321 catggcagtg gtggatggaa ggctggatga gcttcctttg caatggtcag acgacttctg
     7381 cctgggcata tgcgccatat ccggccgggc gatcaagccc ctctcatcca ccggtccgga
     7441 gcggcccggt tatccgggcg agcactacac caatatgcct atcaacggcc tggagcgagt
     7501 ggacccggag gtcataatct atcacaatgg caccgcctgg ggcaccgact ctgcgggcaa
     7561 caggaggctg tacaccaccg gcggccgggt cttcaccctg gtcgcccggg gcaagagcct
     7621 ggccgaggcc agggcaaaag catatgagaa catcaaacgg gtccggttca acggaatgcg
     7681 ctaccgaaag gatatcggcc tgagatatat ctgaatggat cgaggagcaa gatcgagaat
     7741 gatcatattt ggcctggaag gaactgcctg gaacctcagc gccgcactgg tggatgagtc
     7801 gggggtgatc tatgagaaga gcgctactta caccccggcc agaggaggaa tccatccccg
     7861 agaggcctca cagcaccatg ccgagcacat gagggcagtg gtgggagatg tcctcgccca
     7921 ggctcggcag aggggactga agctggaggg ggtggccttc tcccagggac cgggcctggg
     7981 gccttgcctg cgcacagtag ccactgccgc ccgggccctc tcattgcgtt ttgatattcc
     8041 tctggtgggg gttaaccact gcgtcgccca tatcgaggta ggcaaatggc agtcaggtgc
     8101 ccgcgatccc gcggtgatct acgtgagcgg cgccaactcc caggtcctgg ccctgcgcca
     8161 gggaagatac aggatattcg gcgaaaccct ggatatcagc gtgggaaatg ccatcgacaa
     8221 gttcgcccgc tctgtgggcc ttgcccatcc cggcggcccg aaggtggagg agctggctcg
     8281 gaaagcgaaa aattacattc ccctccccta tactgtgaag ggcatggacc tctccttctc
     8341 cggcctgtcc accgccgcaa ccgaggcagc aggaaagcat gatctggaag acgtctgcta
     8401 tagcctgcag gagactgcct tcgccatgct ggtggaggtg acggagaggg ccatggccca
     8461 tgccgagaag agggaggcca tgctggtggg aggagtggga gcgaacgccc gcctggggga
     8521 gatgatgaga atcatgtgcc acgagagggg agcggagttc ttcttaccgc cccgctcgtt
     8581 catgggggac aacggcagca tgatcgccta caccggcctc ttgatgctca agagcggcat
     8641 ctctacccct ctggaccaat cccatgttcg gcccgggtac aggacggacg aggttcctgt
     8701 ctcctgggcc tgatcatcgg ccctatttcc ccggttccgg cccctctgcc tgcgatcttg
     8761 agcgccagtt ctcttctttt ggaggctcca tgtcaagcag gacgaagtat ctgccctcat
     8821 gcggagggcg gtcgatctcc agatcacccc ggccagttgt gccctcgatg caggacgata
     8881 tggtgagatt gatcctgccc agaatggcgg aatcattgcc tgcagagagg gagttcatgt
     8941 cagccaggtc ggcctcaagg agggtggagt tgttcgagcc gggaagagaa ctgacattga
     9001 tggccgcata aagtctccgg tcgaagatca ggctcccccg gtggacctcg tctcccacat
     9061 tctcccggat gtcatccctg atctccttca ggctcttgtt ggaggccacc agctcgcgga
     9121 tctgctcagg ctgcacgggc agcagaacct ccaccttcaa tcggatggga tgagactcat
     9181 ttcccctaat ggcaaacccg tggccgctaa acagcagatc aaccggcaga cccataggga
     9241 tatctggatt ccgatagaga tcctcgccgc ctcttctctc cgggctcatc tgatcattgc
     9301 cgccatctaa tccttccatc tgatcggcct gaaccgcatc cacaaggcca acagatatca
     9361 ggcctgccag cagcaccgcc agagccggat agagaattcc tctgctaact tcattcatcg
     9421 agattcgcct gccaaatacc tactgcaact caatcaattt aaaacatctc caaagggagg
     9481 aaaaagggat ggacggcaga ctgcaactct gccgtgcacc atttcggggc gaatgttatg
     9541 tacgctcagg ccacaagacg gcggtactgg atcgagaccc agttgatgat tccctctttg
     9601 gcctgcatat ctttggcctc atctaccagc tcatttatgg tcatattgtt ctgggcctcc
     9661 accttcgtct tccactgatc ccggatctca ttgagggtca tgttctcaag ctcattggtc
     9721 ttctgatcat atagatccag gatctgattg aggctcatct cgcccagctc ctcactagtg
     9781 acgtcatcca tgagcgatat aggggtaaag ccgcatccca agatgccaaa cccgcaaaat
     9841 ccggcaggcg aagcaccccg gaagtccatc cactccccgc ccctggcatt catccgtccg
     9901 caaaatggca tctcctcttc taaagagctg ttaagagcgc tcatttccag ctcctttatc
     9961 tcccccaggg tcatgtttcc cagctcctct gaggtgacat tgagtggagc agagatcatc
    10021 atcccacgtg catgcattcc cattcctcga tccatgggca tggataatgc cccagatatg
    10081 agcagacagg atgccgccag ggcaatcaga gccatcctcc aattaagttc gttcaacttc
    10141 atctac
//
LOCUS       DAOSGP010000018        10137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5644_length_10137_cov_17.142333, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000018 DAOSGP010000000
VERSION     DAOSGP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5644_length_10137_cov_17.142333"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(653..1576)
                     /gene="mtrH"
                     /locus_tag="PLV49_01145"
     CDS             complement(653..1576)
                     /gene="mtrH"
                     /locus_tag="PLV49_01145"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HOG97474.1"
                     /translation="MFRFKNEQTIVNVAGVRFGGQPGELPTVLCGTIFYQGHRIVEDD
                     ERGIFDRAAAERLVLRQSELSSETGCPFVLHIYARTVPALRKYLDFAEEVWSGPFIVD
                     SAERSTRSLALSLLSELGYADKAIYNSISIATDDAEARALLENEVDSAIILAYNPGEP
                     GVEGAMRILENGGLVRDEGLIPLAKRLGFSNILIDPGVVPMGSGAGSSLRFSVVAKSR
                     LGLPVGSGIHNAVSSWQWLKDRMKSNRSCCDAAAACMQLAMGGDFLLYGPIESGEVIF
                     PAAAMADILVAEAVKDLEVYPEATHPIFRLV"
     gene            complement(1581..2201)
                     /locus_tag="PLV49_01150"
     CDS             complement(1581..2201)
                     /locus_tag="PLV49_01150"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HOG97475.1"
                     /translation="MAEECKKIECDYANQGYLDPWPPVRGEYRLGDPRAGVAVVTLAS
                     VFNPRGTAISGPCKTENLGLEKIVANVISNSNIRFLIVCGVESKGHLPGNTLLALHKN
                     GIDEKGRIIGSKGAIPFIQNLSVQAVERFQRQVELIDRIGLEDMKEIERLVSLYNHLS
                     MPFPEEPYQVIKRKAARQEMLSGDSDLTFGARVSMDTTRWLVASEG"
     gene            2410..2811
                     /locus_tag="PLV49_01155"
     CDS             2410..2811
                     /locus_tag="PLV49_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HOG97476.1"
                     /translation="MASYKERILEVIGESEVGLTTVDVAKKARVSKTTVIKYLSVLSS
                     EGEVEFVEVGPSKLWRTRNTSARHGEGKEGAMNAEAFPMMDIDFHSGEEEIVYFSFRV
                     KTEQICKLMKRAKRCGCEATEIDGGYINAKQ"
     gene            2798..4168
                     /gene="gvpD"
                     /locus_tag="PLV49_01160"
     CDS             2798..4168
                     /gene="gvpD"
                     /locus_tag="PLV49_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD"
                     /protein_id="HOG97477.1"
                     /translation="MQSNDQPSIPKEVKEFFHIDKGQTLLIKGMPGTGKTTLALEIMS
                     SLCEERNGIYISTRVDPARLYATNPWIEKVLMPRNVVNATQTKLLGSLNKTSKDLSNY
                     YAVMDFFRLFFEETEEMDNPMIIIDSWDAVLNYTSHLIGTSQHSFEQNICEFARDLDT
                     HLVFVSEFADVMPLDYIVDGVVTMEQFNLPGHAGGNMRNRYVRQIKLEKLRGVEINQK
                     TYTVTLHNGRFRFFEPYREHNDARIMSDGVRVPDPSEDRISTGIPDLDIVTGGLKYGS
                     CNVLEINHGVGKRYYRMLTALASNAIKNGRGVFILPSIGYQLSSRDIFVPTNVIVSQP
                     EGDDAVAWGKDLLEKWDALRERTGRPILNIIGLDSIEFSFGYKESLNLTNLMIRNWKE
                     TNDINVLVVKSGQESMNMAIHTADSYFLVNELNGGLCLYGVIPRTEPYNMILEDRNRI
                     SLTPIV"
     gene            complement(4252..6237)
                     /gene="acs"
                     /locus_tag="PLV49_01165"
     CDS             complement(4252..6237)
                     /gene="acs"
                     /locus_tag="PLV49_01165"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOG97478.1"
                     /translation="MKSDTARTAVLQEETRIFNTPQWIIEHSNSYQWMKKKGFKTEDE
                     MRKWCSDNYIEFWDEMARTFVDWFKPYTQILDDSEKPHYKWFTGGKVNVAYNAVDRHA
                     HSEKRNKVAYYFVGEPVGDTKAITYYQLYREVNRLANALKSVGVEKGDRVVTYLPMIP
                     EQPITMLACAKIGAIHTLVFSGFSSGGLASRVNDAEAKVVVTADGFYRRGKPLPLKPN
                     VDEALKTSPSVKKVIVVKRTGVSVPMTEGRDVYYDDFVAGQSTKCETVPLDPEDRLFI
                     LYTSGTTGKPKGIEHAHGGYAVGAAQTTSWVLDVHDDDVYWCTADAGWITGHSYVIYG
                     PLILGASSILYEGSPDFPDLGRWWSIIEEYGVSVFYSAPTAVRMFMKSGEEFVKKYDL
                     SSIRILASVGEPLNPEAYMWFRKNIGSDQAPIMDTWWQTETGCHVVAPLAMTPEKPGS
                     VCFPLPGMNTDIYDEDANSVPLGYGGNIVQTAPWPSMLRAFFRDEVRYKKEYWDMYWQ
                     VKPGTYLAGDKATRDKDGYWWIQGRIDDVLKVAGHRISNAEVESAAVSHPKVAEAAVI
                     GEPDEVKGEKIVAFIILKEGVVEEESLKKDISTHVRKVLGPVAYPDKVYFVKDVPKTR
                     SGKIMRRVIKAKALGKDTGDVSALSNPEAVSSIPQIN"
     gene            6384..6599
                     /locus_tag="PLV49_01170"
     CDS             6384..6599
                     /locus_tag="PLV49_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOG97479.1"
                     /translation="MKQLSVLVEDDVLEAIEKRILASGRSKSDVVRDLLIIGLKKPPT
                     SAYRDLVMDAIAAHEKRFHATEKTEES"
     gene            complement(6628..7146)
                     /locus_tag="PLV49_01175"
     CDS             complement(6628..7146)
                     /locus_tag="PLV49_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG97480.1"
                     /translation="MKENREIFVRLERCMGCRSCEMACAVEHSTSKNLISSISEKPVP
                     VRLLYVEKAEGRNVPMICRHCEDAPCVAVCRTGALVQDSISGVVERIEENCMGCWMCA
                     MICPYGVVGRKKEVRMAVKCDRCKSLEVPACVAACPTGALIYSTQREFAEMMRKEAAA
                     RIAREARGLAKA"
     gene            complement(7152..9002)
                     /gene="cooS"
                     /locus_tag="PLV49_01180"
     CDS             complement(7152..9002)
                     /gene="cooS"
                     /locus_tag="PLV49_01180"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HOG97481.1"
                     /translation="MTNDVRSKSMDPACQEMLRACEKSGLETAWDRLEKQQPQCGFGE
                     LGICCRNCNMGPCRIDPFGEGASTGVCGATADIIVARNLLRSIAAGAAAHSDHGRDVA
                     HAFQLIVEGKAPSYDIKDQEKLDRLMKEYGVDSPALLAAAILAEFGKQDGPIQFTRRV
                     PPQRLELWKKLGIDPRGVDREIVECMHRTHIGVDNDYVNLILHGLRTSISDGWGGSMI
                     ATELQDVIFGTPQPIFSEANMGVLEPEKVNIIIHGHEPLLSEMIIAAASDPEMIALAR
                     NEGAEGINLAGICCTANEALMRQGVPVAGNFLSQELVIATGAVEAMVVDVQCIMPALG
                     TMAGCFHTKLISTSPKAKFPGATHMEFHEDTAGETAREIVRTAVKNFRNRDRSRVQIP
                     DIKTRCMAGFSVEAIVSALGGSLDPLIDAIKSGSIRGIGAVVGCNNPKITQDSGHIGM
                     VQELIKNNVLVLTTGCNAQACAKAGLLQPEAAEKAGDGLKGVCQALGVPPVLHMGSCV
                     DISRILVAAAALANALGVDISDLPAAGAAPEWMSEKAVSIGAYVVSSGVFTVLGTVPP
                     VMGSKAVADLLTSGAKDVVGACFAVEPDPVNAAALMIEHIDSQRKKLGLA"
     gene            complement(9160..9975)
                     /locus_tag="PLV49_01185"
     CDS             complement(9160..9975)
                     /locus_tag="PLV49_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HOG97482.1"
                     /translation="MIDLADWIYRYYIHPIIYDTSYNPVDTITWAVVLGLAILGLVKL
                     FRHYHLAIDERMVLSTLPYILAGSSLRVIEDAEIVQPPWSYLLITPLIFFLVFLVTAG
                     SLIITRRLLGDQFHHGYAAIGLAWTLFNLMLLFGVGLENGWVIGAVFLLGSALTGGLL
                     LSRRAISGLSFLDDRFNLMIVYAHMLDASSTYLGVDWFGYYEKHVVPTFLIDLAGTAA
                     VMYPLKLIVLLPALFMIDKAIDDRSLRNLTKLALITLGLAPAVRNTLRLALGV"
BASE COUNT         2320 a         2801 c         2689 g         2327 t
ORIGIN      
        1 tcttgcagct ccctgcggca gtcttccagg gttttgccgg ttgcccatac gccctgaagc
       61 tcgggcacct cgccataaaa gggctcaggg tcatcgatga tctcgtaagt ggcctggtcc
      121 attgctgcct ggatgtattc tgcgaacatc gcaataatat cttcagcgat cagataaagc
      181 ctttcgcatt ttaatcactt gctcatttac ctgccgcccg ggcgggcccc cgtgcacgtg
      241 cacgcgcgct cttgcgggag tgggtgccag gcattgtgcg gggcactcgt tcgggcactt
      301 acctatgccg ccatagtgaa tctagataaa gcgcaaaatc atttgacata acctttaatt
      361 atcataaaaa cctttgccgg cgatccggtt cacagcgttt gggcctatag cgagttgaac
      421 gggtaggcgg tcttgatcac ggtctaccgt ccagatccga atcgatggat cgactgcaaa
      481 gtgaggaaga aatgaatagc ataggtccaa gctggagcga aaagaaacag cggagttcca
      541 ggagatcgga gtctcatatc aggtagcatg agaccaaagg atagaccata ggtctcagta
      601 gctggctagg aatttcgccc ggcactgctc taaggaatgg atcatttcca atctaaacca
      661 ggcggaagat gggatgggtg gcctcgggat agacctccag atcctttacc gcctctgcca
      721 ccagaatatc ggccatggcc gctgccggaa agatgacctc gccgctctca atgggcccat
      781 agaggagaaa gtcgcctccc atggcaagct gcatgcaggc tgcagcggca tcgcagcagc
      841 ttcggttgct cttcatccta tccttgagcc actgccagga gctgactgca ttatgaattc
      901 ccgagccaac aggcagtccg aggcgtgatt tggcaaccac cgagaagcgc agagaagacc
      961 ccgctccgct gcccatcggc accacccctg gatcgatgag aatgttgctg aacccaagcc
     1021 tctttgccag gggtatcagt ccctcgtctc tcaccaggcc accattctcc aagattcgca
     1081 tggctccttc cacccccggc tcgccgggat tgtaagcgag gatgatggcg ctgtcaacct
     1141 cattctccag caatgcccgg gcctcggcat catctgtggc aatgcttatg ctgttgtata
     1201 tggccttgtc tgcgtacccc aattccgaga gcagcgatag ggctaaagag cgagtgctcc
     1261 tctctgccga gtcgacgata aacggccccg accagacctc ttccgcaaaa tccagatact
     1321 tccgcagggc gggaacggtc cgggcataga tatgtaggac aaaagggcaa ccggtctcac
     1381 tggaaagctc agattggcgc agaactagtc tctccgctgc ggcccgatcg aatatacccc
     1441 gctcatcatc ctccacaatc ctgtgcccct gatagaagat ggtgccgcaa aggacggttg
     1501 gcagctctcc aggctggcca ccaaacctta ccccggccac attcactatg gtctgctcgt
     1561 tcttaaaccg gaacatgatc tcatccctcg gaagcaacca accatctggt cgtgtccatg
     1621 ctgacccttg ctccaaatgt cagatcgcta tcgcctgata acatctcctg acgggccgcc
     1681 tttctcttga tcacctggta gggctcctct ggaaaaggca ttgagaggtg attgtatagg
     1741 gagactagcc tttcaatctc tttcatgtcc tctaggccaa tcctgtctat aagctccacc
     1801 tgccgctgaa aacgctcgac ggcctgaacg gagaggttct gaatgaaggg tatcgctccc
     1861 ttcgatccga ttatcctccc tttttcatcg attccatttt tatggagagc gagcagagta
     1921 tttcctggca gatggccctt cgactccact ccgcagacga tgaggaacct tatgttgctg
     1981 ttgctgatca cattggccac tatcttctcc aatcccaggt tctcagtctt gcagggtccg
     2041 gatatggctg tacccctggg gttgaagacg ctggctaaag tgaccaccgc cactcctgct
     2101 ctggggtcgc ctaaccgata ttcgcctcga accggcggcc agggatccag atacccctga
     2161 ttcgcatagt cacattcgat ttttttgcat tcctcagcca tatggccccc gcagatctga
     2221 tgatggattg atccgcatat ctttctgggt tgactgatcg gcatataatt gtttctgatt
     2281 tccacaagag tgtctcgtac ttaaatggct atgaaaaatt gaaatatcat gatacaggat
     2341 aaacatgcgt gatctgaaat ggaaactact tcgcaggaat ttggtcatac aaagagatgg
     2401 tggcactaaa tggcctcata caaggaaaga atactggaag tcatagggga gtctgaggtg
     2461 ggcctgacca cggttgatgt ggccaaaaag gcaagggtca gcaagactac agtgattaag
     2521 tatctctctg tgctcagctc cgagggagag gtcgagttcg tggaggtggg accttcaaag
     2581 ctctggagga cgagaaacac gagcgcaagg catggggaag ggaaggaagg ggcgatgaat
     2641 gcggaggcat ttcccatgat ggacatagat tttcatagcg gcgaggaaga gattgtatat
     2701 ttctcatttc gggtcaaaac ggaacagata tgcaaactga tgaagcgtgc caagagatgc
     2761 ggttgtgagg ccacagagat tgatggagga tatattaatg caaagcaatg atcagccctc
     2821 catacctaag gaggtcaaag aattctttca tatcgataaa ggccagacgc ttctcatcaa
     2881 gggcatgccg gggacgggca agaccaccct cgccctggag atcatgagca gcctgtgcga
     2941 ggagagaaac ggaatataca tatccactcg agtggatccg gcgcggctat atgccacaaa
     3001 tccctggatc gagaaggttc tcatgcccag gaacgtggtc aatgccactc aaaccaagct
     3061 cttgggtagc ctgaataaga cgagcaagga tctatccaac tattatgcgg tcatggactt
     3121 cttccggctc ttcttcgagg agacggagga gatggacaat cccatgatca tcatcgatag
     3181 ctgggacgca gtcctcaact acacctccca cttgataggc acctctcagc acagcttcga
     3241 gcagaacatc tgcgagtttg cccgtgatct ggacactcac ctcgttttcg tctccgagtt
     3301 cgctgatgtt atgcccctgg actacatcgt cgatggggtg gtgaccatgg agcagttcaa
     3361 tctgcccggc cacgccggag ggaacatgag gaaccgctac gtccggcaga tcaagctgga
     3421 aaagctgcgc ggggtcgaga tcaaccagaa gacctatacc gtcacattgc acaatggccg
     3481 gttcaggttt tttgagccct atcgggagca taatgatgcg cgcatcatga gcgatggggt
     3541 tagagttccc gatccctctg aagaccggat atctaccggc attccagacc tggatatcgt
     3601 caccggcggc ctgaagtacg gctcgtgcaa cgtcctggag ataaatcatg gagtgggcaa
     3661 gaggtactac cggatgctga ccgccttggc ctcaaatgcc atcaagaacg gccggggagt
     3721 gttcatcctg cccagcatcg gctatcagct tagctctcgg gacatatttg tgcccaccaa
     3781 tgtcatcgtc tcccagccgg agggggatga cgcagtggcc tggggaaagg atctgctgga
     3841 gaagtgggat gcattgcgag aaaggaccgg ccgtcccatt ctgaacataa tcggcctgga
     3901 ctccattgag ttctccttcg gctacaagga atcgctgaat ctgaccaacc tgatgatcag
     3961 aaactggaag gagaccaacg acatcaatgt gctggtggta aagagcggcc aggagagcat
     4021 gaacatggcc attcacaccg cagacagcta tttcctggta aatgagctga acggagggct
     4081 ctgcctctat ggggtcatac ccaggactga gccctacaat atgattttgg aggacaggaa
     4141 caggatcagc ctgacgccca ttgtataggt gggcggagca agtccagtta ttgcaggatt
     4201 ccatggaaaa ggctcgtttc aacggcgaaa aaaaggaggt atggtttctg gctaattaat
     4261 ctgtggtatg ctgcttaccg cctccggatt ggatagtgct gatacatcac ccgtatcttt
     4321 gcccagcgcc ttggccttga tgacgcggcg catgatcttg ccggacctgg tcttgggcac
     4381 atccttgacg aaataaacct tgtccggata agcaaccggg cccaggacct tccttacgtg
     4441 agtggagata tccttcttga gcgactcctc ctcaaccact ccttccttga gaataatgaa
     4501 tgcaacgatc ttctcgccct tgacctcatc gggctcaccg ataacagcgg cctctgcgac
     4561 cttgggatga gatactgcag cggactcaac ctcagcgttg gatattctat gtcctgctac
     4621 cttcagcaca tcatctattc tgccctggat ccaccagtat ccgtctttgt cacgggtggc
     4681 cttgtctcca gccaggtatg tgccgggctt gacctgccag tacatatccc agtactcctt
     4741 cttgtacctg acttcatctc tgaagaaggc gcggagcatg gacggccagg gggccgtttg
     4801 gacgatattg cccccgtagc ccagtgggac cgagttggca tcctcgtcgt agatatcggt
     4861 gttcatgccg ggcagcggaa agcagacaga acccggcttc tcaggtgtca tggccagcgg
     4921 tgcaacgaca tggcatcccg tctcggtctg ccaccaggta tccatgatag gtgcctggtc
     4981 ggagcctatg ttctttctga accacatgta ggcctctgga ttaagcggct cgcctacaga
     5041 tgccagaatt ctgatgctgg acaggtcgta cttcttgacg aattcctcac cggatttcat
     5101 aaacatcctt acagcggtgg gtgcgctgta gaatacagag acgccatact cctcaatgat
     5161 ggaccaccag cggcccagat ccgggaagtc gggagagcct tcgtacagga tgctggaggc
     5221 acccaggatc agcggaccgt agatcacgta ggagtgtccg gtgatccagc ctgcatcagc
     5281 cgtgcaccag tagacgtcat catcgtggac atccagcacc catgaggttg tctgggctgc
     5341 accaacagca tatccgccgt gagcgtgctc aatacctttg ggcttgccgg ttgtgccgga
     5401 ggtgtacaga atgaacagcc tatcctccgg atcaagcggg acggtttcac acttggtgga
     5461 ctggccagct acgaaatcgt cataatagac atctcggccc tcagtcatgg gcacagagac
     5521 tcctgtcctc ttgactacta tcaccttctt aacggagggt gaggtcttca aggcctcatc
     5581 gacattaggc ttgaggggca ggggttttcc gcgcctgtag aagccgtcgg cagtgacgac
     5641 gaccttggct tcggcatcgt tgaccctgct ggccaagccg cctgaggaga atccagagaa
     5701 caccagtgta tgaatagctc cgatcttggc gcatgccagc atggtgatgg gctgctctgg
     5761 aatcatagga aggtacgtaa caactcggtc tcccttctct actcccacgc tcttgagggc
     5821 gttggccagc ctgttgacct ctctgtagag ctgatagtag gtgatagctt ttgtatcgcc
     5881 cacgggctcg ccgacgaagt agtaagctac tttgttgcgt ttctcggagt gagcatgcct
     5941 gtcaactgca ttgtaggcca cattgacctt gccgccagta aaccacttgt aatggggctt
     6001 ttcggaatcg tcaagaatct gggtataggg cttgaaccaa tcgacgaagg tccgggccat
     6061 ctcatcccag aattctatgt aattgtccga gcaccacttg cgcatttcat cctcagtctt
     6121 gaagcccttc ttcttcatcc actgatacga atttgagtgt tcaataatcc attgcggcgt
     6181 attgaaaatc cttgtctctt cctggagaac agccgttctt gccgtatccg atttcatgat
     6241 ataacatacc ttattatggt atattaggat ttcgctccat ctcttatttc aaactattca
     6301 tcatccgaaa agaaacgtat ctgcagccgt ttctgcggcc atcacgaaaa ctttatcaat
     6361 caaatattat gttatattat cttatgaaac aattatctgt actagtggaa gatgatgttc
     6421 tagaggctat cgagaagcgc atattggcct ctggccgcag caagagcgac gtcgtcaggg
     6481 atctcctaat aatcggcctg aaaaaacctc cgacttccgc atacagagat cttgtaatgg
     6541 atgccattgc agcacatgaa aagagattcc acgcaacgga gaaaactgag gaatcatgaa
     6601 gaataagaga tgcaaaaata aaaaaattta tgccttggcc agccccctgg cctcgcgggc
     6661 tatgcgagct gcggcctcct ttctcatcat ctccgcaaac tccctctggg tggaatagat
     6721 caatgcaccc gtggggcatg cggccacaca ggcaggaacc tccaggctct tgcagcggtc
     6781 gcatttaacc gccattctca cctctttctt ccggcctaca actccgtagg ggcagatcat
     6841 ggcgcacatc cagcagccca tgcagttctc ttcaatgcgt tccaccacac cgcttatcga
     6901 gtcctgaacc agggctccgg tgcggcagac ggcaacgcag ggggcatcct cgcagtgccg
     6961 gcagatcatg ggcacattcc tgccctctgc cttttccaca taaagcaggc ggacgggcac
     7021 aggcttctcc gagatagaag aaatgaggtt cttggaggtg gagtgctcaa ccgcgcaggc
     7081 catctcgcag gatcggcagc ccatgcacct ctccaagcgg acgaatatct cccgattctc
     7141 cttcatcgca atcacgccag gcccagcttc ttgcgctggc tatcgatatg ctcaatcatc
     7201 aaggcagccg cgttcaccgg atcgggctcc acggcaaagc atgctcctac cacgtccttt
     7261 gcgccggagg tgagaagatc ggccactgct ttgcttccca tgacaggcgg aacggttccg
     7321 aggaccgtga acactccaga actgaccaca tatgcgccga tggataccgc tttctcgctc
     7381 atccactcag gagctgcccc tgctgccggc aggtcgctta tgtccactcc cagagcattg
     7441 gccagggcgg cggcagcgac cagaatgcgg ctgatgtcca cgcatgagcc catgtgaagg
     7501 accgggggga ctcccagggc ctggcagact cccttcaggc cgtcgcccgc cttctctgcc
     7561 gcttccggct ggaggaggcc cgccttggca caggcctggg cgttgcagcc cgtggtgagg
     7621 accaggacgt tgttcttgat cagttcctga accatgccga tatgtccaga gtcctgggtg
     7681 atcttgggat tgttgcagcc caccacggcg cctatgcccc tgatgctgcc gctcttgatg
     7741 gcgtctatca gcgggtctaa gctgcctccc aaagcggaga ctatcgcctc caccgagaag
     7801 ccggccatgc atcgggtttt gatatcgggg atctggaccc gggaacgatc gcggttgcgg
     7861 aagtttttca ccgccgtacg cacgatttcc ctggccgtct ccccagcagt atcctcatgg
     7921 aactccatgt gggtcgcgcc ggggaacttg gctttgggtg aggtggatat caatttggta
     7981 tggaagcagc cagccattgt gcccagggca ggcatgatgc actgcacatc caccaccatc
     8041 gcctccaccg cgcccgtggc tataaccagc tcctgactga gaaagttgcc tgctaccgga
     8101 acgccctggc gcatcagagc ctcgttggcc gtgcagcata tccctgccag gttgattccc
     8161 tctgccccct catttcgtgc cagagcgatc atctccggat ctgatgctgc agcgatgatc
     8221 atctcagaga ggaggggctc gtggccatgg atgatgatgt tcaccttctc cggctccagg
     8281 acacccatgt tggcctcgga gaatatcggt tgaggggtgc cgaagatgac atcctgcagc
     8341 tcagtggcga tcattgagcc tccccagccg tcagagatgc tggtcctgag gccatgcagt
     8401 atgaggttca cataatcgtt gtccacgccg atatgagttc ggtgcatgca ttccacgatc
     8461 tccctgtcca cgccccgggg gtcgattccc agcttcttcc acagctcgag cctttggggc
     8521 ggaacccggc gggtgaactg gatggggccg tcctgttttc caaattctgc caatatggct
     8581 gctgccagga gggcagggct gtccactccg tactctttca tcagccgatc gagcttctcc
     8641 tggtccttga tgtcatagga gggggctttg ccctccacta tgagctggaa ggcatgggct
     8701 acatccctcc cgtggtcgga gtgggctgct gccccggcgg caatgctcct cagtaggttt
     8761 cgcgccacga ttatgtctgc agtggccccg cagacgcccg tgcttgctcc ctccccaaat
     8821 ggatcaattc gacagggtcc catgttacag tttctgcaac atatgcccag ctcgccaaaa
     8881 ccgcattgag gctgctgttt ttccagtcta tcccaggctg tctccaggcc ggacttctca
     8941 caagcccgta gcatctcctg gcaggcaggg tccattgatt tgctgcgaac atcattggtc
     9001 attttccaat ccctctatag tgagcacaag tagcgacgga atcaccattt aactatttaa
     9061 tcatggtgtt ttggtcggag agaatttgct gaaacagcga ggagccaatt tattcactct
     9121 catagccttg ccacagcgga cttccatatc ttttccattt caaacaccca gcgccagccg
     9181 cagggtgttt cgcaccgccg gggccaggcc cagggtgatc agggccagct tggtgaggtt
     9241 tctcagggat cggtcatcaa tggccttatc gatcataaaa agggcgggca ggagcacgat
     9301 cagcttcagt gggtacatga ccgccgctgt tcctgccaga tcgatgagga acgtggggac
     9361 gacatgcttt tcatagtagc caaaccagtc cactcccagg taggtggagc tggcatcgag
     9421 catatgggca tagacgatca tgaggttgaa cctgtcatcc aaaaaggata ggccagatat
     9481 ggctctgcga gagagcagca atcctccagt cagggcggag ccgagcagaa atactgcccc
     9541 gatgacccag ccgttctcca ggcccacccc gaaaagcagc atgagattga acagggtcca
     9601 ggcaaggcct atggcggcgt agccgtgatg gaattggtcc cccagaagcc ttctggtgat
     9661 gatcagagat ccggcagtga ccaggaagac caggaagaat atgaggggag tgattaatag
     9721 atagctccag ggcggttgca cgatctccgc gtcctctatc accctcaagc tcgagccagc
     9781 caggatgtag ggcagggtag ataagaccat ccgctcatcg atcgccagat ggtagtggcg
     9841 aaagagcttt accagtccca gtatcgccag gcccagcacc accgcccagg tgatcgtgtc
     9901 cactgggttg tagctcgtat catagataat ggggtggatg tagtacctgt atatccagtc
     9961 tgctaaatcg atcacatcag gtcattgcac tctcacagta tttcagccga tcgaaatatg
    10021 gcgtgcaaaa agtatttata ttgcagaacg ccaagcatgc ttgtccattt tccgatttta
    10081 gctgaggtaa ttaaatggtc gaggtgttca aaggtacaac tacggtaggt gtactct
//
LOCUS       DAOSGP010000019        10079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5688_length_10079_cov_17.603252, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000019 DAOSGP010000000
VERSION     DAOSGP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5688_length_10079_cov_17.603252"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..62)
                     /locus_tag="PLV49_01190"
     CDS             complement(<1..62)
                     /locus_tag="PLV49_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG97483.1"
                     /translation="MLLPKTNCKKCGLPTCMAFAV"
     gene            191..1933
                     /locus_tag="PLV49_01195"
     CDS             191..1933
                     /locus_tag="PLV49_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HOG97484.1"
                     /translation="MPFSNPIALLGLLGIIPLVIIYLIRPQPKEIRFSSIQFLREGEA
                     KRTAVLSRLISDPLFWVQLLAICSLSLAAAGPYTNEQGPASSHLVVVLDGSASMQSSF
                     SPAQNIIRSYLDRYERISIILAKNVPQTVLTEGSPAEARDQLPRLVPVAVSADLSSAL
                     TQASILLGSTGGDILLVSDFISWTGDDPDVTRNLIEAGGRVGIVFADSYQGGNNLALT
                     EGWNVPGTGYVNHTALIHNYGPTRTIPITITGPGGKTSQAAAIPQDGDYYLSFTAYPG
                     VNKISLDIEDAISWDNQAYVYVPDQAKKDVLYLGEAGPALKALQSLPNVNVMTSGRYS
                     DFDLIVLARNASADGDLNRYIDGGRVIYLPFDLESPQYLPVRVTGKLPGPTRLWVRNF
                     GFAEGLHFDEIGIYSYPDAAARGGATTMVEANGVPILAYWRLGEGIVIYDGLEMDSDF
                     YLRPEYPIFWYQMINWMTGVPDIEDSNRKTGEIIATGEQSTIQTPSISLIASTIALDE
                     VGIYRFQGRTVAANMYNPTESSLLRSRDFEQGEFLGVVRNTIVQRDLSPWVIALAALA
                     IILELAIMRWRRET"
     gene            1930..4290
                     /locus_tag="PLV49_01200"
     CDS             1930..4290
                     /locus_tag="PLV49_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOG97485.1"
                     /translation="MIQDLYFARPELLWALPPLALLGLVYVHLRARNKILALSRLLIF
                     CLILAAAANPYFVQTQVVQSQKPSITVLDDKTGSMTVFDPDVAARVADLTGASLRTFS
                     GDSTPLGDRILQHALPGSTILLVTDGYSNSGRSLDDALALAGSSNSTVFALDIDPVKG
                     DAGVEISGPNTAVLGGDYPFRVMVRSSLYYSGPLSVYADEKKIYSDQIQANGSASIKI
                     SHSFLETGNHILRAVIAPDMQPVNDVYQKSIYVVPKPEVLLLTSSDSSLAQNLDDLYK
                     LTAASELPPSLDSYKSIILDDIEYSSSLDRLEDYVREGGGLVVVGGKNSYDLGEYRNS
                     TLERFLPVQSFPSRFEGGKTLIFVLDISFSLLSTLTKDGTTLLDYEKALAVELLHSPQ
                     FQDYNVGLVVFGTRAYNVIDPVPLSRSQNVLADRIATLAPTGTENSYLDSGLELARDM
                     IQASGGEGEMIVLSDGNLQNYGDVVVRSKELIREMNITTRLIQVQAIPGATGSFPDIA
                     SSTGSEFTYFVYPDSLTTISTPQPEETRPPEKNITSYDLAVVNQNHYITTDLELNATI
                     TGFNDVTPKPGSLRLVALTDGKPVLTVWRYGLGRVAALTTDNGISWAGLLYRSPSSQL
                     ISSVVNWAVGDPRPQEDRVEGEDGWQNTPLQITIASNSRPSLPGANVEKIGDNRYTAT
                     FTPNSSGIYYIGDYGVAVNYPLEYRYIGFNPDLPSLIMANGGKVFTEEEAGRSLIAEA
                     SRVSQRMTEERVSRRDALLLFALFIFLLEIVSRKINDIRRQGRTKP"
     gene            4360..4668
                     /gene="yciH"
                     /locus_tag="PLV49_01205"
     CDS             4360..4668
                     /gene="yciH"
                     /locus_tag="PLV49_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HOG97486.1"
                     /translation="MSSQMCAVCGLPQELCICEEVAKEQQRIVVKVQKRRYGKEVTVI
                     QGIDPHEIDLQELCTHLKSKLACGGTVKDSLIELQGNHIQRIKDILVKKGFGADQIST
                     "
     gene            complement(4782..5144)
                     /locus_tag="PLV49_01210"
     CDS             complement(4782..5144)
                     /locus_tag="PLV49_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOG97487.1"
                     /translation="MSVEEALDYIKSHPEGALQSDLWKALKIDSRKCSRIVAKLLKDR
                     HITREVESVDGIRTYRLFCVSRTSGRRFDPLMALDAFEPCAGCIEECIPEHCASLSEW
                     IFSIVMSSDEVAPSNAIQ"
     gene            complement(5154..>5342)
                     /locus_tag="PLV49_01215"
     CDS             complement(5154..>5342)
                     /locus_tag="PLV49_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HOG97488.1"
                     /translation="SKIQVEMKGSPRYVLEGVLNSVDEYLNLHLLETVELIGGEKTRS
                     LGSVILRGNNIILISPVE"
     assembly_gap    5343..5442
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5443..5628)
                     /locus_tag="PLV49_01220"
     CDS             complement(<5443..5628)
                     /locus_tag="PLV49_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOG97489.1"
                     /translation="MQNLNDEVEAALGKIREGLRVDGGDVELVDISDGIVTVRLQGHC
                     AGCPFSQMTLKNFIEKEL"
     gene            complement(5612..5962)
                     /locus_tag="PLV49_01225"
     CDS             complement(5612..5962)
                     /locus_tag="PLV49_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MJ1244 family protein"
                     /protein_id="HOG97490.1"
                     /translation="MKTMVKIFVDHEHLVRVINSLTELGITGFYLIEYQGMAPTNWKS
                     FQLSEDPDMALKAIRDHSEPGVMVNTVVGSDKCQQLIKELEEALKGIRFTIIGHQVNS
                     VKVKGDDNTHAKPK"
     gene            6058..6537
                     /gene="cobZ"
                     /locus_tag="PLV49_01230"
     CDS             6058..6537
                     /gene="cobZ"
                     /locus_tag="PLV49_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase CobZ"
                     /protein_id="HOG97491.1"
                     /translation="MPSRDIRDVLKSRGITEEEIISAAMELYFPHPGVETRERAEAVF
                     RREMNLALSDPNLALLIYAALLLELEGERRNLPNLKPSDYENDLTCLIADEVIGLSIA
                     KYVGGYKGLFDYVRYDKAKPGILSRLGPFLDDALAGLIGGVSANMYTRAAPDQGSGN"
     gene            6534..>7010
                     /gene="cobS"
                     /locus_tag="PLV49_01235"
     CDS             6534..>7010
                     /gene="cobS"
                     /locus_tag="PLV49_01235"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOG97492.1"
                     /translation="MKGLLFALKSGFGWLSTIPVGISMEGVEALMNHVYVFPLVGLVL
                     GAILGTVSFIALQAFPASLVAVVAIVAIYWLCGINHIDGLADFADGVIAHGTREKKIA
                     AMKDVYMGTGGGLYIVVLILAAFAVLSSLPERLLPLALLTAEVSAKEAMISFAAFSS"
     assembly_gap    7011..7110
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7111..7319
                     /locus_tag="PLV49_01240"
     CDS             <7111..7319
                     /locus_tag="PLV49_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOG97493.1"
                     /translation="RLGRRPDSLFNYRGQLIVPSGINVLDGGEIEREQEDYHLIMERI
                     ELAVNVNVAEDLRLCERILQGDFI"
     gene            7316..7678
                     /locus_tag="PLV49_01245"
     CDS             7316..7678
                     /locus_tag="PLV49_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97494.1"
                     /translation="MNDTITINGEKFSLGDLMLLTGEDEVKEPKGYILLVARALRNPM
                     RLPWLLKEICNLCIKEDEQRDMRLSLIRVQVDAELKMNQDIQRFQQRRYVAQVIEILL
                     FNDLMLAPREAVEEGDME"
     gene            complement(7863..8312)
                     /locus_tag="PLV49_01250"
     CDS             complement(7863..8312)
                     /locus_tag="PLV49_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97495.1"
                     /translation="MAKIFSIMVLATMLALIAPAIAQLPSEAVASNREDIPAINRSII
                     NQFDPTYVWMNPYALDLSGISPAVFGNIAFAKSAGGMSSALYTESINEFLSRDPDAGK
                     SNITVNAARVGLTTKAAFVGSSVRGSPSAPGSTAASPSVAEMQNETT"
     gene            complement(8463..9515)
                     /locus_tag="PLV49_01255"
     CDS             complement(8463..9515)
                     /locus_tag="PLV49_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOG97496.1"
                     /translation="MKTMGKTYSTGLTVSPAEIQHNMAMFVAMAKLLRAVEYPSSFDW
                     RSIEGKDYTTPIRDQGKCSSSVAFATIALMESMIKIANQDPKLQPDLSEAYLFPRGGG
                     KCESGAQFGRMLTAAESGVCDELCCPYAGDWMPCPDFKSRLTAITFSEMIFSPEQAKA
                     HIASTGPLMSGLAVYADFFDHDGGIYSQDNGDFVGNHAVLIVGYDEGEDCWIGKNSWG
                     PNWGEGGWFRIKQGECGIGSAFPFYSAAIGLSPAPTSPPSPDIAAPIDGTLYVTMTRA
                     GERDVILLVNNREIGALTLGETLTAGEFKRGDTIQFDLVGIAHKNVCFPSGWRIWTLR
                     MDGGKYEFRVLERDKR"
     gene            9794..>10079
                     /locus_tag="PLV49_01260"
     CDS             9794..>10079
                     /locus_tag="PLV49_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97497.1"
                     /translation="MTGRWAKVRRTDMQMVFVLMLLSCLSIQAIALDPGTIAVPSVTG
                     ANMDMPKPLITPPSMDMANSPTKVTVVEGKRDPFSDGSPFNETETSEADKV"
BASE COUNT         2280 a         2791 c         2603 g         2205 t
ORIGIN      
        1 acggcaaaag ccatgcaggt gggaagcccg cacttcttgc agttggtctt gggcaatagc
       61 atataaatct ccatggcgcc taccataacc agatacctca gtttgggaag gatacagcag
      121 taacatcaat ctcgatcata tttattgcct gcgcaaagct tttcatactt cttcaggaat
      181 tgtcttttca atgcccttca gcaatcccat tgcccttttg ggcctccttg gcatcatacc
      241 cctggtaata atctacctca tccggcccca gccaaaggag atccgcttct cctccatcca
      301 gttcttgcga gagggggagg cgaagcgcac tgccgtcctc agccgtctga taagcgatcc
      361 cctcttctgg gtgcagctct tggccatctg ctctctctcc ctcgccgccg ccgggcccta
      421 caccaacgag caggggccgg caagcagcca tctggtagta gtcctggacg gctctgcgag
      481 catgcagtcc tcattctccc ccgcccagaa catcatccgt tcatacctcg accgctatga
      541 gagaatcagc atcatcttag caaaaaacgt tccacaaacg gtcctcaccg agggcagtcc
      601 ggccgaggcc cgagatcaac tcccacgact tgtgcctgtt gcagtctcag cagacctctc
      661 cagcgcactg actcaggcga gcatactcct gggcagcacc gggggggata ttcttctggt
      721 ctcggacttc atcagctgga ctggagacga tccagatgtc acccgcaatc tcatcgaagc
      781 cggcggccgg gtgggaatag tctttgccga ctcataccag ggagggaaca acctggccct
      841 gacagagggc tggaatgtgc cgggaacggg atacgtaaac catactgctc tgattcacaa
      901 ctacggcccg acaagaacca tacccattac catcaccggc ccgggcggca agaccagcca
      961 ggcagctgca atcccccagg acggcgacta ctatctatcc ttcaccgcct atcccggggt
     1021 gaataagatc tctctggaca ttgaggacgc catctcctgg gacaaccagg cttatgtcta
     1081 tgtgccagac caggccaaga aggacgttct ttatctgggc gaggccgggc cagccctaaa
     1141 agccctccag tccctgccca atgtaaatgt tatgacctca ggcaggtact cggacttcga
     1201 tctgatcgtc ctggcaagga acgcctcggc cgacggcgat ctcaaccgct acatcgacgg
     1261 cggccgggtg atctacctcc ccttcgatct tgagagcccc cagtatctcc cggtgagggt
     1321 gacgggaaag ctccccggcc ccaccaggct ttgggtgaga aattttggtt ttgccgaggg
     1381 cctgcacttc gatgagatcg ggatttacag ctacccggac gctgctgctc gcgggggcgc
     1441 gaccaccatg gtggaagcca atggtgtccc catcctcgcc tactggcggc tgggcgaggg
     1501 gattgtgatc tatgacggcc tggagatgga ctcagacttc tatctgcgcc ccgaataccc
     1561 catattctgg tatcagatga tcaactggat gacgggggtg ccggatatag aagactccaa
     1621 ccggaagacc ggggagatca tagccacagg cgagcaatcc accatccaaa cccccagcat
     1681 cagcctcatc gccagcacta ttgccctgga cgaggtgggc atctatcgct tccagggacg
     1741 aacggtggcg gcgaacatgt acaaccccac tgagtcctcc ctcctccgca gccgagattt
     1801 cgagcagggt gaatttctag gagtggttcg caataccatt gtacagaggg atctatcccc
     1861 ctgggtgata gccttagccg ccctggccat cattctggag ctggccatca tgcgctggag
     1921 gagggagaca tgatccagga tctctatttc gctcggccgg agcttctctg ggccctgcca
     1981 cccctagccc tgctggggct ggtctacgtc caccttcggg ccaggaataa gatcctcgcc
     2041 ctctccaggc tgctgatctt ctgcttgata ctggcagcgg ctgcaaaccc ctatttcgtt
     2101 caaacccagg tcgtccaatc gcaaaagccg agcatcaccg tcctggatga caagaccgga
     2161 tcgatgacgg tattcgatcc cgatgtggcg gcacgagttg ccgatctgac cggggcttct
     2221 ttgcgcacat tctccggtga ctcgactccc ctgggggaca ggatcctcca gcatgccctt
     2281 ccaggaagca caatcctcct ggtgacggat ggctacagca atagcggccg atcgctggat
     2341 gatgccctcg ccctggccgg gagctcgaat agcactgtct ttgccctgga catcgatccg
     2401 gtgaaaggcg atgctggggt ggagatatca gggccgaaca ccgctgtcct gggaggggac
     2461 taccccttcc gggtgatggt tagaagctcc ctttactaca gcgggcccct atcggtctat
     2521 gccgatgaga agaagatcta cagcgaccag atccaggcca atgggagcgc ctcgatcaag
     2581 atctctcatt ccttcctgga gacgggaaac catatcctca gagcggtgat cgccccggat
     2641 atgcagccgg taaacgacgt ttaccagaag tcgatatatg tcgtccccaa gccggaggtt
     2701 ctcctcctca cctcaagcga ctcgtccctg gcccagaacc tggatgatct ttataagctg
     2761 actgcagcct ctgagcttcc ccccagcctg gatagctata agtccattat ccttgatgat
     2821 atcgaataca gctcgagcct cgaccggctg gaggattatg tgcgcgaggg aggcggcctg
     2881 gtggtagtgg gaggcaagaa ttcctacgac ctgggagaat accgcaactc gactctggag
     2941 aggttcctgc ctgtgcagtc ctttcccagc cgctttgagg gaggcaagac gctcatcttc
     3001 gtcctggaca tctccttcag cctgctgtcc accctcacca aggacgggac caccctgcta
     3061 gactatgaga aagccctggc ggtggagctg ttgcattccc cccagttcca ggattacaat
     3121 gtgggactgg tggtctttgg aacccgggcc tataatgtga tcgatcccgt gcccttatcc
     3181 cggagccaga acgtcctcgc tgacagaatc gctacccttg cccccacagg aacggaaaac
     3241 agctatcttg actctggcct cgagctggcc agagatatga tccaggccag cggaggcgag
     3301 ggggagatga ttgtcctctc cgatggaaac ctccagaact atggggatgt cgtagtacga
     3361 tccaaggagc tgatccggga gatgaacatc accaccaggc tgattcaggt ccaggccatc
     3421 cctggcgcga cgggtagctt cccggatatc gccagcagca cgggctcgga gttcacctat
     3481 ttcgtctatc cagactctct gaccaccatc agcacccctc agcccgagga gacgaggccg
     3541 ccggagaaga acatcactag ctacgacctg gcagtggtca accagaacca ctatatcacc
     3601 accgatctgg agctgaacgc caccatcact ggcttcaatg atgtcacccc caaacccggc
     3661 tctttgaggc tggtggcact gaccgatggc aagcccgtcc tcaccgtctg gcgttacggc
     3721 ctggggaggg tagcggccct gaccacagac aacggcattt cctgggccgg tctgctatat
     3781 cgcagcccca gctctcagct gatatcctcc gtcgtcaact gggcggtggg cgatccccgg
     3841 ccgcaagaag acagagtgga gggagaggat ggttggcaga acactcccct gcagatcacc
     3901 attgccagca actcccggcc cagcctgccc ggggcgaacg tggaaaagat aggagataat
     3961 agatacacag cgacgtttac tcccaacagc agcggcatct actatatagg agactacgga
     4021 gttgctgtca actaccccct ggagtacaga tatatcggat tcaatccaga tcttcccagc
     4081 ctgatcatgg ccaatggagg caaggtcttc accgaggagg aagcggggag gagcctgatt
     4141 gcagaagcat ctcgggtcag ccagaggatg acagaagaga gggtttccag aagagatgcc
     4201 ctgctcctct tcgccctctt tatattcctg ttggagattg tatcgagaaa gatcaatgac
     4261 atacgaaggc aggggcggac gaagccgtga ggtcaccatc agattttaat agcataatca
     4321 gcctgtagtg agatatatta tggaggtaac tgccaccaaa tgagttctca aatgtgtgcc
     4381 gtctgcggcc ttccacagga gctatgcatc tgcgaggagg ttgccaagga gcagcaaagg
     4441 atcgtagtca aagttcagaa gcgtagatat ggaaaagagg tgacagttat ccagggcatt
     4501 gatcctcatg agatcgatct gcaggagctg tgcactcacc tgaaatccaa gctggcctgc
     4561 ggcgggacgg taaaggacag tctgatcgag cttcaaggca atcatataca aaggatcaag
     4621 gatatcctgg tcaagaaggg attcggcgca gatcagataa gcacctgaat tccattttgc
     4681 aggtttttat cgatgggcag gcagatccta acttgcttat tttctttgct taaaaagatt
     4741 gattatgatg agggagagcg caatgcctgg ctgcctccat cctattgaat tgcattggaa
     4801 ggagccacct cgtctgagct cataacaatt gagaatatcc actcgctcag gctggcgcaa
     4861 tgctcgggaa tgcattcctc tatgcagccg gcgcagggct caaaggcatc cagagccata
     4921 agaggatcga aacgtctgcc gctagtcctg ctgacgcaaa agagcctata ggtcctgatg
     4981 ccgtcgactg actccacctc tcgggtaata tggcgatctt tgagcagctt ggccactatg
     5041 cgagagcatt tcctggaatc gatcttcagt gccttccata gatcgctttg cagggctccc
     5101 tcaggatgcg acttaatgta atctaatgct tcctcgacag acatccccta cactcactcc
     5161 actgggctga ttagaatgat gttattgccg cgcaggatga ccgatcccaa agagcgagtc
     5221 ttctcgcccc cgatcagctc cacggtctcc agcaggtgca ggttcaagta ctcatctaca
     5281 ctgttgagaa caccctccag cacgtatcgg ggtgatccct tcatctctac ctgaatcttg
     5341 ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngagctcct tctcgatgaa
     5461 gttcttcagg gtcatctggg agaatggaca gcccgcacaa tgcccctgca gccttacggt
     5521 cacaatgcca tcactgatat cgacgagctc aacatctccg ccgtccacgc gaaggccctc
     5581 ccggatcttt cccagggccg cctccacctc gtcatttagg ttttgcatga gtattatcgt
     5641 ctcctttaac ctttacggaa ttaacctggt gcccgattat ggtaaacctt atccctttta
     5701 aagcttcctc gagctctttt atgagctgct gacacttatc tgagcctaca acggtattga
     5761 ccatgactcc gggctctgaa tgatctcgga tggccttcaa tgccatatcg ggatcctcgc
     5821 tcagctggaa gctcttccag ttggtgggtg ccattccttg atactcaatg aggtaaaagc
     5881 cggtgattcc aagctccgtc aagctgttga tgactctgac gagatgctcg tgatctacaa
     5941 aaatcttgac catggtcttc atggagtttc ctcacccata tatcggctat aagaccttat
     6001 aaaacttcgc gataccggga tattctttta tcctacccag gctatgcata ccctaccatg
     6061 ccatccagag atatcagaga cgtgctgaag agcaggggaa tcactgagga agagatcata
     6121 tctgcagcaa tggagctata cttcccccat cccggagtgg agacgcgcga gcgagcagaa
     6181 gccgtattcc ggcgggagat gaacctagct ctgtccgacc ccaatctggc cctgctcata
     6241 tacgccgccc tcctcctgga gttggaggga gaaaggcgca accttcccaa cctgaaaccg
     6301 tccgactatg agaacgatct gacctgcctt atcgcggacg aggtcatagg cctgagcata
     6361 gccaagtacg tgggcggata taaggggctg ttcgattatg tgcgctacga caaggccaaa
     6421 ccgggaatac tctcccggct gggacccttc ctggatgatg ctctggccgg gcttatcggc
     6481 ggagtctcag ccaacatgta caccagagcg gcgccggatc aggggagcgg gaattgaaag
     6541 gcctcttgtt cgcccttaaa tccggtttcg gatggttgtc caccataccg gtaggcatat
     6601 ccatggaggg ggtggaagcc ctgatgaacc atgtctatgt ctttcccctg gtgggcttgg
     6661 ttttgggggc aatcctggga acggtatcct tcatcgctct gcaggcattt ccggcgagcc
     6721 tggttgccgt agtggcaata gtcgctatct actggctctg cgggataaac cacatcgatg
     6781 gcctggcaga ctttgctgat ggtgtgatcg ctcacggtac aagggagaag aagatcgctg
     6841 cgatgaagga cgtatacatg ggaaccggag gaggactata tatcgtcgtt ttaatcctgg
     6901 cggcatttgc cgtcctgtca agcctgccgg aaaggctctt gcccctggca cttctcaccg
     6961 cagaggtcag tgccaaagag gctatgatat cctttgctgc cttctccagc nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccgtctggg ccggaggccc gactccctct
     7141 tcaactaccg gggccagctg atcgtgccct cggggatcaa tgtgctggac ggaggagaga
     7201 tcgagagaga gcaggaggat tatcatctga taatggagag aatagagctg gcagtgaatg
     7261 tgaatgtggc agaggacctc cgcctctgcg agagaatact gcaaggtgat tttatttgaa
     7321 tgacaccata acgattaacg gcgagaagtt ctctctcgga gacctgatgt tgctcaccgg
     7381 cgaggatgag gtaaaagagc cgaagggcta tattctcctc gtggcccgag cattgcgtaa
     7441 ccccatgagg cttccctggc tgctcaaaga aatatgcaac ctctgcatca aggaggatga
     7501 gcagagggat atgaggctta gcctgatccg agtgcaggtc gatgccgagc tgaagatgaa
     7561 ccaggatatc cagagattcc agcagaggcg ttatgttgcc caggtgattg agatcctgct
     7621 gttcaacgat ctgatgcttg cccccaggga ggcagtggaa gaaggcgata tggagtagct
     7681 gatggctgcg gccagccacc ggtccatctg gcgcgcagga gcgaaaaacg atctgatttc
     7741 caggaggagt ccctgccgag aagcccttct ggccttaaag ccccatttct ggcatgggac
     7801 gacgcctgga tcatgaggca gcatgccggg cagaagcctg gcagatcctc acaaactcag
     7861 atctaagtgg tctcattctg catttctgcg acgctcggtg acgctgcagt gctcccaggt
     7921 gcggatgggc tccctcgcac actgcttcca acaaaagcgg ccttagtcgt caatcccact
     7981 ctggctgcat tgacggtgat attgctcttt ccggcatcag gatcgcggct caaaaactca
     8041 ttgatgctct cggtatatag tgccgatgac atgccaccag cgctcttggc gaaggcgatg
     8101 ttcccaaata ctgccggact gattccggat agatccaaag catatggatt catccataca
     8161 tacgtgggat caaattgatt tattatgctc ctgtttattg caggaatatc ttcgcgatta
     8221 ctagctactg cctcagaagg cagctgggca attgcaggcg ctatcaatgc cagcatggtt
     8281 gccaggacca ttatgctaaa aatctttgcc atattaatcc tcctaaatgc gcatcctgcc
     8341 ctcattccca ctccctggca gagatgcgat cggtggtaaa ttgatgctgc tatcgcggga
     8401 gctgaacttc atttcaacac cgcagatagc ggttcgaact ataatgataa taaaccttgt
     8461 ggttatcgtt tatcccgctc cagaacccgg aactcgtact ttcctccatc catcctgaga
     8521 gtccatatcc tccagcccga tgggaagcag acgttcttat gagcgattcc cacaaggtcg
     8581 aactggattg tatcacccct cttgaactcc ccagcagtca gggtctcgcc taaggtgagc
     8641 gctccgatct ccctattgtt caccagcaag atcacatcgc gttctccggc tctggtcatg
     8701 gttacataca aggtgccgtc gatcggggcg gcgatgtctg gcgacggtgg cgaggttgga
     8761 gcaggactca agcctatggc agcggaatag aatggaaaag ctgagcctat gccgcactct
     8821 ccctgcttga tcctgaacca tcctccttcc ccccaattgg ggccccagga gttcttccct
     8881 atccagcaat cctccccttc gtcatagcca acgatcagaa cggcgtggtt gccaacgaag
     8941 tccccattat cctgactgta gatcccgcca tcatggtcga agaagtctgc atagaccgcc
     9001 aggccgctca tgagagggcc ggttgaggcg atgtgggctt tagcctgctc cgggctgaag
     9061 atcatctcag agaatgtgat ggcagtcaat ctgctcttga aatccgggca tggcatccag
     9121 tctccagcat aaggacagca gagctcatcg caaacccctg actccgcagc cgtgagcatc
     9181 cgcccgaatt gagcaccgct ctcgcacttt ccgcctcctc ggggaaaaag ataggcctca
     9241 gagagatcgg gctgcaattt ggggtcctga tttgcgatct ttatcatgga ctccatcagg
     9301 gcgatggtgg cgaaggcaac ggagctgctg cattttccct gatcgcggat gggggtggta
     9361 tagtctttgc cctcaatact cctccagtca aaggaagagg gatactctac cgccctcaga
     9421 agctttgcca tggcaacaaa catggccata ttatgctgaa tctctgctgg cgatactgtc
     9481 agcccggtcg aataggtctt tcccattgtc ttcatctcca atttatggca attctgcccg
     9541 catcgctacc caccgcccag gctattggtg gctaagacga tttgtagtcg aaatatccaa
     9601 tagtataata ggctggatga aatatatact tggctatgaa gtacaaaatt ttttaacggt
     9661 tgggtggatg aactcataat cgaccataaa cgcttgaact ccttgggaat tgtccgcttt
     9721 ttccaggaat gccaaccgac tggacgtcga cgcctcttcc ccgggataac atttaaaatc
     9781 gtgacgaaga taattgacag gcaggtgggc aaaagtgaga cgaactgaca tgcagatggt
     9841 atttgttttg atgctgctat catgcttatc gattcaggcc attgccctgg atcccggcac
     9901 catcgcagtg cccagtgtca ccggagccaa catggatatg cccaagcccc tgatcactcc
     9961 ccccagcatg gatatggcca attcccccac caaagtcaca gtggtggagg ggaagagaga
    10021 tccgttctcg gatggatcgc cattcaatga gacagaaacc agcgaagcag ataaagtga
//
LOCUS       DAOSGP010000020         9938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5798_length_9938_cov_19.156228, whole genome shotgun sequence.
ACCESSION   DAOSGP010000020 DAOSGP010000000
VERSION     DAOSGP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5798_length_9938_cov_19.156228"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1269)
                     /locus_tag="PLV49_01265"
     CDS             complement(52..1269)
                     /locus_tag="PLV49_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOG97498.1"
                     /translation="MLVNLDLHIHSRYSMAVSGDMELGQIAKGAAHKGVKIVGTGDCL
                     HPLWLESIKKLPEDDGLRRQGDTLFLLSVEVEDSKRVHHLILLPDTGKAEELAEGFSP
                     HSGNLHQDGRPRLHMSGAQIADLVFEAEGLIGPAHAFTPWTGMYAYHRSLSECYQEKA
                     DRIRFIELGLSADSDYADRIAELSDKTFLSNSDAHSPRTNKLAREFNQMEVEGCSYQE
                     IALAICREKGRGPTLNAGFFPGEGKYNRTACTRCFHHFSAKEMNDLMGRCSHCQGQIK
                     LGVADRIDLLADYEGPQHPSHRPPYLHLIPLAEIIALALGYKSPMTVGVQRLYAAMTE
                     DKTEIEVLLNEHLGELEAAKVEPKVIGAIEAFRNGGVEVLPGGGGKYGEIRLLASKKA
                     GGEREPQRSLFDF"
     gene            1284..2123
                     /gene="larB"
                     /locus_tag="PLV49_01270"
     CDS             1284..2123
                     /gene="larB"
                     /locus_tag="PLV49_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOG97499.1"
                     /translation="MKIFSERDKPFMLMAPLPDEKMESNREILESYRRGEISLEEALK
                     QIRVRDYRTVGEVAKLDICRSNRIGIPEAILAEGKDKSDLLAISLAHLEETGSVIITR
                     VSSEQLVVLRSAKLPPKSVFEHNPRARTVVIRSADRPERVGIVAILAAGTADIPVAEE
                     ARVVAEEMGCQVLREYDVGVAGIHRLFPCLERMADADAFVVAAGREGTLPAVVAGLVN
                     APVIGLPVSTGYGIGGGGKAALYSMLQSCSVLTVVNVDAGFVAGAYAAKIARQKAHKE
                     KQS"
     assembly_gap    2233..2332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2383..3606)
                     /locus_tag="PLV49_01275"
     CDS             complement(2383..3606)
                     /locus_tag="PLV49_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG97500.1"
                     /translation="MATILETRGIEYAYADGTRALSNVSLTIEEGRKIALVGPNGAGK
                     STLMLMFNGILRPDAGEVLFRNERLDYDSRSLRKVRQRVGMVFQNSDDQLFAPTVYQD
                     VAFGPVNLSFPPEKVKRYVGYALQYVGLSGYERRPPHHLSGGEKKRAAIAGILAMEPE
                     VMILDEPTSNLDPATSEEIMEMLDELNLGGKTLIISTHDVDLAYRWADEVVLMKDGGV
                     LRRGSAEEVFGDLELVRDARLKPPMVVDLYQELVNRGLAEGKKPPKSILELCDSLEGG
                     ERRCAVQGAKRGTIYVCNADELLLENANELVVKVDADYTGAMGTRAKLMAEEAKIRVD
                     FTYGVIDKCILKALAGKSCLVLTSQGMMGQVQKRIEAYSQQSGEEINSVDLSSGSNGR
                     SLPIDRDFVGTKSLK"
     gene            complement(3609..4403)
                     /gene="cbiQ"
                     /locus_tag="PLV49_01280"
     CDS             complement(3609..4403)
                     /gene="cbiQ"
                     /locus_tag="PLV49_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOG97501.1"
                     /translation="MHDLLDDWAQCNALRETSAILKLFLGLGAIIICVSSVTPSAPLF
                     VAASMSLIILILAKIPIRIYSRLLLLPLSFALLSSTVVALMHGSGEMLYSMQLFGLEI
                     GVREDGAALALLLIARTFGGMCSLFFIALTTPMIEIFAVLKSLRIPESVIELSMMIYR
                     YIFVFLDQAAMIHSAQVMRLGNLGTRSALSSFSMLASVLFLRSWEQGERLIVAMDARC
                     YDGRLDLMEERKGAGALEIAATAAFLALALAISLMTGEEGILRMGG"
     gene            complement(4396..4719)
                     /locus_tag="PLV49_01285"
     CDS             complement(4396..4719)
                     /locus_tag="PLV49_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG97502.1"
                     /translation="MKLEIIVLVVVALFTAIFVYQSTSGEHEWAGADDQSEKVIDELT
                     GGAYEPWSSPLWEPPSGEIESLLFALQAAIGSLVIGYFLGYYRGLMRKKGEQRDGDGE
                     ARSDA"
     gene            complement(4716..5402)
                     /locus_tag="PLV49_01290"
     CDS             complement(4716..5402)
                     /locus_tag="PLV49_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOG97503.1"
                     /translation="MEGFLPHPWWEIWLIISLPVVAWGVYKMNKMVKENREILPLLAV
                     SGAFVFVLSSLKMPSVTGSCSHPTGTGMGAILFGPWITAVLSAIVLIYQALFLAHGGL
                     TTLGANIFSMGIAGPLIGYLVFVLAKRSGLNMYLSVFLAAMLADWTTYVVTSMQLALA
                     FPAASGGVVASFQAFMAIFAITQVPLAVVEGAVTALMFKYLVRLRGDILVKLNVASAS
                     AIKLLREAAT"
     gene            5530..>6419
                     /gene="xseA"
                     /locus_tag="PLV49_01295"
     CDS             5530..>6419
                     /gene="xseA"
                     /locus_tag="PLV49_01295"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOG97504.1"
                     /translation="MTQIFTVTNLNERIRERLENDPRLHDLWAQGEVSNFVHHRSGHM
                     YFTLKDRDSQISCVLFKGNIGQVETELRNGMNVLLFGDVAVYRPQGRVQLVARAIKRD
                     AGLGFRFLQFEALKKKLGEEGLFDQEQKRPLPLYPERIGIVTSLQGAALRDVVRTIGP
                     YPARIIISPARVQGEKAEESIVSALLALQGRTDLIIVCRGGGSAEDLWCFNSEVVARA
                     VFACDSPVISAVGHETDVTITDFAADMRAATPTAAAKIAVPDVADLKIRLEQSQVRIM
                     RALWTSLERKEERLEYIQRSL"
     assembly_gap    6420..6519
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6526..6657
                     /gene="xseB"
                     /locus_tag="PLV49_01300"
     CDS             6526..6657
                     /gene="xseB"
                     /locus_tag="PLV49_01300"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOG97505.1"
                     /translation="MSLEKSLELYERGMRLVRVCNLRLDSAQRRIECLTGELPSDLL"
     gene            6752..7936
                     /gene="trpB"
                     /locus_tag="PLV49_01305"
     CDS             6752..7936
                     /gene="trpB"
                     /locus_tag="PLV49_01305"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOG97506.1"
                     /translation="MMIDNFAVKFGRYGGLFVPETLVAPLADLSIAYDRLKKSPQFQD
                     ELKALQKDFAGRPTSLYLAPNLSRKLGCRVYLKREDLVHGGAHKLNNTLGQALVAKKM
                     GKTRLIAETGAGQHGVATAIVGAKLGFETQVYMGEVDIERQKMNVYRMELMGAEVIPV
                     RSGSRTLKDAINEALRDWASSFEHTHYLLGTAAGAHPFPSMVRDFQSVIGQEAREQIR
                     EAEGRLPDSIVACVGGGSNAMGIFAPFLKDDVRLIAVEAGGRGPGMSDKMADNGASLG
                     YGTDGILHGALTKILQDPYGQILESYSVAAGLDYPGVGPELAFLAEKGRVLPRLADDR
                     TALEGFRALSRLEGIIPALESAHAIGYALAEPESLGDLSIINVSGRGDKDLATVMDYE
                     TI"
     gene            7923..8699
                     /gene="trpA"
                     /locus_tag="PLV49_01310"
     CDS             7923..8699
                     /gene="trpA"
                     /locus_tag="PLV49_01310"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOG97507.1"
                     /translation="MRLSEAFEKGPLLIVYLCSGDPSPGATPELVRRLVRAGADIIEL
                     GLPHSDPIADGPTIQAAAQRAIAAGMNTDVYFQVAEEADVPVPKVFMGYYNMVYARGL
                     ERFARDCARCGITGMIVPDLPPEEAAPLKEACGKHGVDLIYLVAPNTPPARIDLIAEE
                     TGGFLYLVARTGVTGARSEVLQDTRDLIARVPGKKPKAVGFGISTPDQAAEVIRAGAD
                     AAIVGSACVDLIARGRIDELEALVGEMKKAVKAAGRARLQ"
     gene            8749..9366
                     /locus_tag="PLV49_01315"
     CDS             8749..9366
                     /locus_tag="PLV49_01315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOG97508.1"
                     /translation="MNEELRKYVERTRRDIESGADATIVCFGDSITAGYAVRRGFPSF
                     LLESLRQRFPDSKIEMINSGISGDTSQDGLGRLDWAVLSYEPDLVTINFGINDCVLGL
                     SLEEFEMNLVEMVRRIRAGPDSEILLLSSQPLESPPYDQRVLDYYQTVERVAKEMNVG
                     FVDVYGAWMKRVRAGMPLDSLILPGLDHPNEAGYKIIAEELISLF"
     gene            9454..>9938
                     /locus_tag="PLV49_01320"
     CDS             9454..>9938
                     /locus_tag="PLV49_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOG97509.1"
                     /translation="MLLIFFALSLGGAAAAPAGVGADIVKIISVNYQGADQWVEIANM
                     GSGLMNLDGWSLKNRENQSYAFPANFTLKAGSTARVHSKEGSNSSSDLYNSTLSWSEM
                     GDTATLEDIAGRIISQYRYPIEVSTPGSASDNKPLSLPNAFSSGGMNPPFLPAYRPPP
                     G"
BASE COUNT         2125 a         2742 c         2813 g         2058 t
ORIGIN      
        1 gataagagca atgaggcgat tacaatacag acgcgatccg tctcaatctt attaaaaatc
       61 aaaaagggac ctctgcggtt ctctttctcc cccggctttc ttcgaagcta aaagccggat
      121 ctctccgtat tttcctcctc cgccgggcag aacctccact ccgccgttgc ggaaggcctc
      181 aatggcaccg atcaccttcg gctcgacttt tgccgcctcc agctccccga gatgctcgtt
      241 cagcaatacc tcaatctccg tcttatcctc tgtcatggcc gcgtacagcc tctgcacccc
      301 caccgtcatc gggctcttgt agcccagggc gagggcgatg atctctgcca gaggaatgag
      361 gtgcaagtaa gggggccgat gagagggatg ctggggccct tcgtagtcgg ccaatagatc
      421 gatccggtca gccaccccca gcttgatctg cccctggcaa tgggagcagc gtcccatgag
      481 atcattcatc tcttttgcgc tgaagtgatg aaagcagcgg gtgcaggccg tgcggttata
      541 ctttccctct cccgggaaaa aaccggcatt gagcgtcggt cctcttccct tctccctgca
      601 gatggccaaa gctatctcct ggtagctgca cccctccacc tccatctgat tgaactctct
      661 ggccagctta ttggtgcgag gtgaatgggc atcggagttg gagagaaagg tcttgtccga
      721 gagctccgca atcctgtccg cataatcgct gtccgcgctc agtccaagct caatgaatcg
      781 aatccggtcc gccttctcct ggtagcattc agatagggac cgatggtaag catacatccc
      841 cgtccagggg gtgaaggcat gggcgggccc aatgaggccc tccgcttcaa acaccaggtc
      901 ggcgatctgg gccccgctca tatgcaaccg cggcctgccg tcctgatgca ggttgcccga
      961 atggggtgaa aatccctccg ccagctcctc cgctttgccg gtgtccggca ggagaataag
     1021 atgatggacc ctctttgaat cctccacctc cacgctcagg agaaagagag tatccccctg
     1081 cctcctcagc ccatcgtcct ccggcagctt tttgatggat tcgagccaca gagggtgcag
     1141 gcagtcgcca gtgcccacta tcttcacccc tttatgcgcc gccccctttg cgatctgccc
     1201 cagctccatg tcgccggata cagccatcga atagcgggaa tgaatgtgca gatcgagatt
     1261 gactagcatc ttcccaactc ctgatgaaga tcttctcgga gagggataaa ccctttatgc
     1321 tcatggctcc attaccggat gagaaaatgg agagcaacag agagatcctg gagagctacc
     1381 gccggggaga gatttccctg gaggaggccc taaagcagat tcgggtcagg gattatcgca
     1441 ccgtgggcga ggtggcaaag ctggatatct gccgatcaaa tcgcattggc ataccggagg
     1501 cgattctcgc cgagggaaag gacaagtccg atcttctggc catatccctt gcccatctgg
     1561 aggagacggg gagcgtgatc atcacccgtg tgtcctccga gcagctggtt gtattgagat
     1621 cggccaagct tccccccaag agcgtctttg agcacaatcc tcgcgccagg acggtggtca
     1681 tccgctctgc tgatcggccg gaaagggtgg gcatcgtggc catcctggcg gcaggaactg
     1741 cggacatccc cgtggctgag gaggcaaggg tggtagcgga ggagatgggc tgccaggtgc
     1801 tcagagagta tgatgtgggg gtagcaggca tccaccgcct ctttccgtgt ctagagagga
     1861 tggcggatgc ggatgcattt gtcgtggcgg ctggtcgaga gggcaccctt cccgcggtgg
     1921 tagcgggact ggtgaacgct ccggtcatcg gccttcccgt ctccactgga tacgggattg
     1981 gcggtggggg aaaggctgcc ctctattcga tgctccagtc ctgctccgtc ctgacggtgg
     2041 taaatgtgga tgcgggcttt gtggcgggag catatgccgc caagatcgcc agacaaaaag
     2101 ctcataaaga gaaacagtct taaattattt ctaggaaggt atttatacca taaaaaggct
     2161 cgtttgcctt gagcgggtat actcatcttt tttactaacc cccactcccc tacccgctct
     2221 agctattacc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctattcct
     2341 tcatacgcta ttccattaca ctttacttta tgcaaattaa ttttacttaa ggcttttggt
     2401 cccgacaaaa tcgcgatcta ttggcaaaga gcgcccattg ctgcctgagg atagatcgac
     2461 tgaatttatc tcttctccgg actgctgaga ataggcctct atcctctttt gaacctggcc
     2521 catcattccc tgagaggtca agaccaggca gctcttgcct gcaagcgcct tgaggatgca
     2581 tttgtctatc accccgtagg tgaagtccac ccggattttg gcctcttctg ccatcagctt
     2641 ggccctggtc cccatagccc cggtataatc ggcatccact ttcactacca actcatttgc
     2701 attctctaaa agaagctcat cggcattgca gacgtagatc gtgcccctct tcgccccctg
     2761 gacagcacac ctcctctcgc ccccctccag agagtcgcat agctcaagaa tgctctttgg
     2821 aggcttcttg ccctctgcca gccctctgtt cacaagctcc tgatatagat ccaccaccat
     2881 tgggggcttc agcctggcat cacggacgag ctccaaatcg ccgaacacct cctcagccga
     2941 gccccggcgg agcactccac cgtccttcat cagcaccacc tcatccgccc agcgataggc
     3001 caggtccaca tcgtgggtgg agatgatcaa tgtcttcccc cccaggttaa gctcatcgag
     3061 catctccatg atctcctctg atgtggcggg atcgaggtta gaggtgggct cgtccaggat
     3121 catcacctcc ggctccatgg ccagaattcc cgcaatggcc gccctcttct tctccccgcc
     3181 gctcaggtga tggggagggc ggcgctcgta tccagatagg ccgacatact gcagggcata
     3241 gcccacatac cttttcacct tctccggagg gaaactcaag ttcaccggcc cgaaggccac
     3301 atcctggtag acggttgggg caaagagctg atcatcggag ttctggaaga ccattcccac
     3361 cctttgcctt acctttcgca atgatcggga gtcataatcc agcctctcat tccggaatag
     3421 aacctcgcct gcatccggcc ttaagatccc attgaacatc agcatcagag tggactttcc
     3481 cgctccattg gggcccacca gggcaatctt tctgccctcc tctattgtga ggctgacatt
     3541 gctaagggcc cgcgtgccgt cggcataggc gtattctatc ccccttgtct ccaggatcgt
     3601 ggccattctc accctcccat ccttagaatc ccttcttctc cagtcataag gctgatcgcc
     3661 agggccaagg ccaaaaaggc cgcagtggct gctatctcta atgctcccgc tccttttctc
     3721 tcttccatga gatcgagccg gccgtcgtag cagcgggcgt ccatggccac gatcagcctc
     3781 tctccctgct cccaggatct gaggaataga acgctggcga gcatggagaa ggagctgagg
     3841 gcactcctgg tgcccaggtt ccccagtcgc atcacctggg cgctgtgtat catcgctgcc
     3901 tgatcgagaa agacaaatat gtatcggtag atcatcatag agagctctat caccgattcg
     3961 ggaatccgaa gcgacttgag gacggcgaat atctcgatca ttggggtggt gagggcgatg
     4021 aagaacaatg agcacatgcc gccgaaggtt cgggcgataa gaagcagggc caaagctgcc
     4081 ccatcctctc ggacccctat ctccaggccg aaaagctgca tggaatagag catctctcca
     4141 gagccatgca tcagggccac caccgttgag ctgagcagag cgaaggagag ggggagaagg
     4201 aggagccggg agtagatgcg aatgggtatt ttggccagga tcaggatgat gaggctcatg
     4261 ctcgcggcga cgaataaggg cgcggatggc gtgaccgagg agacgcagat tatgatcgct
     4321 cccaggccaa ggaatagctt aagaatggcg ctggtctctc tcagggcatt gcactgggcc
     4381 cagtcatcca ggagatcatg catcagatct tgcttccccg tccccgtctc tctgctcgcc
     4441 tttctttctc atcaaacctc tgtagtatcc caggaaatag ccgatgacca ggctccctat
     4501 ggccgcctgg agggcgaaga gaaggctctc gatctcgccg ctcggcggct cccatagggg
     4561 acttgaccag ggctcataag cccctccggt caattcatcg atgaccttct ccgattgatc
     4621 gtccgcccct gcccactcgt gctctccgct ggtgctctgg tagacgaaga tggcagtaaa
     4681 aagggcaaca accaccagga cgattatctc cagcttcatg tagcagcctc ccggagcaat
     4741 ttgatggcgg aggcactggc cacattgagc ttcaccagga tgtcgcccct gagccggaca
     4801 agatatttga acatcagcgc agtcaccgct ccctccacca cggctaatgg aacctgggtg
     4861 atggcgaata tggccatgaa agcctggaag gaggccacga ccccacccga ggcggcggga
     4921 aaagccaggg cgagctgcat tgaggtcacc acatatgtgg tccagtcggc gagcatggcc
     4981 gccaggaaga cggagaggta catattcagc ccagacctct tggcaagaac gaagaccaga
     5041 tatccgatca ggggaccagc gatgcccatg gagaagatgt tggcccccag agtagtcaag
     5101 ccgccatgag cgaggaatag ggcctgatag atcagcacga ttgccgaaag gactgcggtt
     5161 atccatggtc caaagaggat cgctcccatg cccgtccccg tggggtgtga gcagctccca
     5221 gtgactgagg gcatcttaag ggatgagaga acgaagacga aggccccaga gacggcgagc
     5281 aggggcaata tctcccggtt ttccttgacc atcttgttca tcttataaac gccccaggcc
     5341 accaccggca gagagataat gagccagatc tcccaccagg gatggggaag aaagccttcc
     5401 attatatgca tcacaacacc cgtgtgcagc agggatattc tagcatataa actttctttg
     5461 ccttatgacc agattaattt aataaattga gaaatgctct taaataatcc gctctataaa
     5521 aggagatcat tgacccagat attcactgtc accaacctca atgagagaat tcgagagcgt
     5581 ctggagaacg atccccgcct gcatgatctc tgggcgcaag gagaggtatc caacttcgtc
     5641 catcaccgct ccggccatat gtacttcacc ctgaaggacc gggattccca gatctcctgc
     5701 gtcctcttca aagggaacat tggccaggtg gagacggaat tgagaaatgg catgaatgtc
     5761 ctcttgtttg gggatgtggc ggtctaccgt cctcagggga gagtgcagct ggtggccagg
     5821 gccattaaga gggatgccgg tcttggcttt cggttcttgc agttcgaggc cctgaaaaag
     5881 aagctggggg aagaggggct cttcgatcag gagcagaagc gccctcttcc cctctatccg
     5941 gagaggatag ggattgtcac ctctctgcag ggtgctgcgc tgagggacgt ggtgcgcacc
     6001 atcggcccct atccagcaag gatcataatc tctccggcca gggttcaggg ggagaaggct
     6061 gaggagagca tcgtctctgc acttctcgcc ctgcagggca ggacggacct gatcatcgtc
     6121 tgtcgcggag gcggctcagc cgaggacctg tggtgcttca actcggaggt ggtggcaaga
     6181 gcagtcttcg catgcgacag tccggtgatc tccgccgtgg gccatgagac ggatgtgacc
     6241 atcacagact tcgcggccga tatgcgggcg gcaaccccaa ccgctgccgc gaagattgca
     6301 gtgccggatg tggcggatct gaagatcagg ctggagcaga gccaggttcg gattatgcgc
     6361 gccctttgga caagcctgga gagaaaggag gagcgtctgg agtatatcca gcgcagcctn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggaagatgag cctggagaag
     6541 agcctggagc tgtatgaacg aggcatgagg ctggtccggg tgtgcaacct tcgactggat
     6601 agtgcccaga ggaggatcga gtgcttgacc ggcgagctgc cctccgatct gctctgaggc
     6661 aaagcttaaa taagatctag gatgtacttt tttatacatc atagtattaa ttcgtgcatt
     6721 aaaccattta gctggtgatt cgttgagcag tatgatgatc gataatttcg cagtcaagtt
     6781 cgggaggtat ggcggcctct tcgtccccga gaccctggtg gctcccttgg ccgacctctc
     6841 tattgcctat gaccggctta agaagagccc ccaatttcaa gatgagctga aagctctcca
     6901 aaaggacttc gccggccggc ccacatcgct ctatctcgcc cccaacctca gccggaagct
     6961 gggctgccgg gtgtatctaa agagggagga cctggtgcac ggtggagctc ataaactcaa
     7021 caacaccctt ggccaggctc tggtggcaaa gaagatgggc aagacccgcc tgatcgccga
     7081 gaccggagcc ggccagcatg gcgtggccac ggccattgtg ggggcaaaac tgggctttga
     7141 gacccaggtt tacatgggtg aggtggacat cgagaggcag aagatgaacg tctaccggat
     7201 ggagctgatg ggagcagagg tgataccggt tcgctccggc tccaggacct tgaaggacgc
     7261 cataaacgag gccttgaggg actgggcctc cagcttcgag cacacccact atctgctggg
     7321 aaccgccgcc ggcgcccatc ccttcccctc aatggtcagg gactttcaga gcgtgatcgg
     7381 ccaggaggcc cgggagcaga tccgggaggc ggaaggtcgt ctgccggata gcattgtggc
     7441 ctgcgtgggt ggcgggagca atgccatggg catcttcgct ccattcctga aggacgatgt
     7501 gaggctgatc gcagtggagg caggaggtcg aggcccgggg atgagcgaca agatggcaga
     7561 caatggcgca tctttgggct acggcacaga tggaatactg catggtgccc tcaccaagat
     7621 cctccaggac ccatacggcc agatactgga atcctactca gtggcagcag gccttgacta
     7681 tcccggagtg ggtccggagc tggcattcct ggcggagaag gggcgcgtcc ttcccaggct
     7741 ggcggacgac aggaccgctc tagagggatt ccgcgcccta tcccggctgg aggggatcat
     7801 ccccgcactg gagtcggctc atgccatcgg ctatgcatta gcagagccgg agtcactggg
     7861 cgatctttcg atcatcaatg tctccggccg cggggataag gacctggcta cggtgatgga
     7921 ctatgagact atctgaggcc ttcgagaaag ggcccctgct gatcgtctat ctctgctcag
     7981 gagacccgtc gcccggtgcc acccccgagc tggtgcgccg gctggtgagg gcgggagcgg
     8041 atatcatcga gctggggctc ccccattcag accctatagc agacggtccg accatccagg
     8101 cggcagcgca aagggctatc gctgcgggga tgaacaccga tgtatacttc caggtggcag
     8161 aagaggctga tgtgcccgtc cccaaggtat tcatgggtta ctacaatatg gtctatgccc
     8221 ggggcctgga gaggtttgcc cgggactgtg cccgctgtgg catcaccggc atgatcgttc
     8281 ccgacctgcc cccggaggag gccgcgcccc taaaggaggc ctgcggcaag cacggcgtgg
     8341 acctgatcta cctggtagca cccaacacgc cccctgcacg gatcgatctt atcgctgaag
     8401 agacgggcgg ctttctctat ctggtggcca ggaccggcgt caccggggcg agaagcgagg
     8461 tcttgcagga tacaagagac ctcatcgctc gagtgccggg caaaaagcca aaggcggtgg
     8521 gattcggcat ctccacccct gaccaggcgg ctgaggtcat ccgggcgggg gcggatgccg
     8581 ccatagtggg atcggcctgc gtggacctga tcgccagggg caggatcgat gagctggagg
     8641 cattggttgg ggagatgaaa aaggcggtaa aagctgcggg gagagcaagg ctgcaatagc
     8701 taggactatt gcattgccat gaaagacgaa tgacctgagg gaataccgat gaatgaagag
     8761 ctgaggaagt atgtggaaag gacgcgccgg gacattgagt caggggcgga tgccactatc
     8821 gtatgctttg gtgactccat caccgctggc tatgccgtgc ggcgaggttt tccctccttt
     8881 ttgctggagt cgctccgtca gaggttcccc gattcaaaga tagagatgat caactctggc
     8941 atctccgggg ataccagcca ggacggcctg ggccggttgg actgggcagt gctatcttat
     9001 gagccggatc tggtcaccat aaacttcggc atcaacgact gtgttttagg gctgagcctg
     9061 gaggagttcg agatgaacct ggtggagatg gtgcgccgca tccgggcagg cccggactcg
     9121 gagatcttgc tcttatcctc ccagcccctg gagagccctc cctatgacca gagggtattg
     9181 gactactatc agacggtgga gagggtggca aaggagatga atgtgggctt tgtggacgtc
     9241 tacggcgcct ggatgaagcg ggtgcgagct ggtatgcctc tagattctct gatcttgccg
     9301 ggcttggacc accccaatga ggcgggatac aagataatag ccgaggagct gataagcctc
     9361 ttttaagcaa gtccttgttt gcaggcgaga aggttcgtgg aggtatgagg tatgttaatg
     9421 aaaagcgcgg gtggatgctt gatcagactg gcaatgttat tgatattttt tgctctttcg
     9481 ctgggagggg cggcagccgc tccagcgggc gttggggcag atatcgtcaa gatcataagc
     9541 gtaaactacc agggtgccga tcagtgggtt gagatcgcca acatgggatc tggtttgatg
     9601 aatctggacg gctggtcgct caaaaaccgg gaaaatcaaa gctatgcgtt tcctgcaaac
     9661 ttcaccctga aggccggctc aacagcccga gttcactcca aagagggcag caattcctcc
     9721 tctgatctat acaatagcac gctctcttgg agcgagatgg gcgatactgc cactttagag
     9781 gatatagccg gaaggatcat ttcccagtat agatatccaa tagaggtttc gactcccggt
     9841 agcgcatccg acaacaaacc cctctccctt cccaatgcat tctccagcgg cgggatgaat
     9901 ccgccttttc tgcctgctta tagaccgcct cccggcaa
//
LOCUS       DAOSGP010000021         9781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5920_length_9781_cov_19.615361, whole genome shotgun sequence.
ACCESSION   DAOSGP010000021 DAOSGP010000000
VERSION     DAOSGP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5920_length_9781_cov_19.615361"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..307)
                     /gene="rnpB"
                     /locus_tag="PLV49_01325"
     ncRNA           complement(5..307)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLV49_01325"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            447..1217
                     /locus_tag="PLV49_01330"
     CDS             447..1217
                     /locus_tag="PLV49_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase"
                     /protein_id="HOG97510.1"
                     /translation="MARDQKDHYYRKAKEEGYRARSAYKLLQINEKFHVIKKGDSVVD
                     LGAAPGGWLQVAQKLSGGKIVGVDLEGITPIPGVVTFRADITALSTVDLVKDALGGDA
                     DVVICDAAPNLSGAWDRDHAISIDLARSALEMAKKLLRPRGNFVVKVFQGDMFIDFLN
                     DVRREFAVVHAHSPAASRKESAETYVVGKKLLSAPVRKGDMLNVRIESVGKSGDGVAM
                     VEGFAIIVRGSKLKEELQVKVDAVLTNFAFAEIVERKS"
     gene            1255..>1522
                     /gene="albA"
                     /locus_tag="PLV49_01335"
     CDS             1255..>1522
                     /gene="albA"
                     /locus_tag="PLV49_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HOG97511.1"
                     /translation="MEDDAVIFIGDKPVMNYVLAVMTQFNKPIASVILKARGRAISRA
                     VDTAEITRNRFMPNLTVKNIIIGTESIPNEEGKMVNVSSMEIILT"
     assembly_gap    1523..1622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1624..2937
                     /locus_tag="PLV49_01340"
     CDS             1624..2937
                     /locus_tag="PLV49_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HOG97512.1"
                     /translation="MTVFRAYDVRGVYGTDLTEQLVKRVGESFGSYAEGGSVSLGRDT
                     RISGPSLQKAFLDGVLSTGCQVNSFGIIPIAIISFITWKEELKAAAYISASHNPPEYN
                     GVRFRTCDGYGLLYQESSIMKYYREGGFLAGQGVKTDRDPGEAIERYREYVQEKLNLK
                     RPLKLVLDMGNGAACTMASFYQRLGFDPLILNGTTDGLFPGRGPAPNEESLRPACQML
                     KKKEADFGVGFDPDADRGIVIDDKGAVLPPEKVAIIITRARYKPGDLVISGFDCSMIL
                     ERELEKDGIKVLRERVGDVFVANRVKNSKAVLGVERSGHFFLPEFQYSDDPFAMSLAL
                     AEIVSSGEKLSDLAGMIPDYPYKQMSIHLNEDPAAVMLRLADSLAFFEPDTTDGLKIT
                     TESYSILIRPSNTEPIIRLYIETTKGDMKELEEQYKKIIHQAMKA"
     assembly_gap    3101..3200
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3201..3746
                     /locus_tag="PLV49_01345"
     CDS             <3201..3746
                     /locus_tag="PLV49_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04182 family glycosyltransferase"
                     /protein_id="HOG97513.1"
                     /translation="KRLNMFGNKMINRFFAAIYGISLHDILSGYRAFTTEGIRMLDLS
                     MPGFEIESEMTIESVKKGLRIIEVPITYQPRSAGTKTKLHPFRDGLKIILTIFRMAKT
                     ENPMFYFGLMGSFVGLLGFLVGVYVARDWLLWRIEHIPLTILTAILIIVGIQLFMIGM
                     QGDMMASMHREMMRELYRKKR"
     gene            complement(3770..8110)
                     /locus_tag="PLV49_01350"
     CDS             complement(3770..8110)
                     /locus_tag="PLV49_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOG97514.1"
                     /translation="MKRFYLLLFMAILSATLLSAADDEPLARGVKGEVEDIILVGADD
                     WHASVAATPLAIYSQENETVIKPMLILPKEVHAGERNGWIEESDLERYGASAILDTLK
                     GANISAITIHGSGEKVGALVKAAQKDGIKAYVAVTLEIPVSQASSEALEGLDSNEMAL
                     MAEVGLAITEPDQSTIDRSMLQEPNPEIGGNSSYYCPVNPEVKDRLYNQIEMLIDDYH
                     VDGVVLYRFGFQDENHCYCDVCKEKFYQDTGIDISKVNANSYNRERWAEWKQDQLMDI
                     VMDARNITSDLGPVLLGVALDSPFDRSKGYNFARMTEVADFALISPVSPQDVKLASRE
                     STNPVYVRLNDDYVGYVLSTQNVKGAVSYIEDLVGSGASGVVFEYDVVHTPLWSELEP
                     PSSAASWLLQQLNGEVLAVGNISWRSDSNIRVNNSFDLSEQISRRWASSPGAVIVGEN
                     YTAALQAATIASYLNWPLLFVNEALPEETRSALVRLGAKDAILAGPTGERVRQNLSQM
                     NITLIEGSGDLLVEEMERRNESPSMVVLTNSHDLSLLPPASSKAVERSLVGDLLVRTE
                     LSPDQVPAEEDGEVVRLNFTLTNSGDEVLRGVQLRDIFPMGRLIRLASTAYGEVNVTD
                     PYSRKPATETSAFLNGSLIRWDFGRLEPGESASLNVEVELLYPMDAGWSQRLDTGATA
                     AYEGFSYNHTLRNVDDPPVINLTYPKWIYSGSTNISWNIERNSSYLALNLYSPDERSG
                     RLKLTNITRDGLYDVRVSLQTPGNWLFNIETDEARTENYTIEVRSNVKVLNITAFSHT
                     KVPRLSMVAAGAASSHKAILIDVACDPQKIDPLVVEETLNQKVAELNLDPEYLMVVGD
                     PGSIPFISTGLIQKLSEVMEYEVYRDYQIKPDLNNYSTVAVGRIMGLSVYDASQLMAR
                     TMAYDRLNGSWKNNGLVVSSPALSFPQAPVGIRIKEYLEEAGIMVKDLRHEEATYQQT
                     ISQLNNGQNIAHFDHHGSEDSWGLSAWSMTDSALTGSQVKELTLPPQTTTASACVTTN
                     LKGYYLNVTGTRMYVPGNLEDSIALAFIRAGAVNYIGGSALSYIFLSDDYYKSFYQSL
                     VFRNSTVGQAQLDADNLFRLKSRGTESLKPNSDYDEVLPDWDISVHEMLNQTSYMNVI
                     LGDPSFKPALPKAPSLPYAIVLSAENDTDLNQTILNVAVIPLNESATDWVYWIESKSS
                     SGDLNLDATPAIIGEVLLPKDAEKIVVKEDGLSLWHDEYSRGLEKRVMWPIIRPKLGE
                     KRSFIIEYELIPGQVQRINVTAGWNAVSIYLNPRDASAERYLKNKPYRSIFTIAEDGW
                     NFGMKEAGKLNVTQFKAGEGYLIDSTGNFTMEIPGKPVDLPYRLDLHVGWNMIGLPVN
                     QTVDLANITINAEHKRYRYPEAVEKGLVSAFVWKHEGGDWTNLGENETLEPGRAYLFE
                     AKSEAKLEFR"
     gene            complement(8107..>9781)
                     /locus_tag="PLV49_01355"
     CDS             complement(8107..>9781)
                     /locus_tag="PLV49_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97515.1"
                     /translation="MNSCNPLGKGFKASRCDISLCKDPVYDKWGKMRLIYPIIILFLL
                     IGAGAAQLAPDKERFDIVLHPGDGDERILKVMNNGDSTIFNIAKTEMIGNARDFIFID
                     VPDDKPLSPGEEAEIKIYIGIPPETEPGVYTGFVYLMESAPPSIPVRIDFHVNVVVQE
                     SYGIAMTVDDAKSAYLRASAKDIAQFNLAVKNLGSFRDIASIDAGAMPEGWSVVLMDD
                     DEPMSMPYDLPLNPGATHEIKLQIQTDKPGSRENVTITATSLGNRSMNSSIQAEVEFG
                     MVVRGYDTDILVPEKIATNKSYEGKFKITLDVKEDIWISMHSPDELIVIPQSQMVTVT
                     PEEPGLANFTFLASKAGEYPLTFQLLDSHGIPMPEETATIVAAPPEGLAVLTGDDIIY
                     GTIGTLALFGNRSPSIFMVPADEISDDYLQRLQSYLEIVILGNGSVIPSHVEKKLKDI
                     ELKRIQSDSLFEESWLFTKLIWDNGTSNVVLTTPREADIFRAYKIAMNEGLPMVVCRG
                     NVTGASIEAIEEMTRRNTTLSRAMVVGRVEEDYTKALREAGVLLEEVSA"
BASE COUNT         2239 a         2601 c         2454 g         2287 t
ORIGIN      
        1 atacgagtga ggagagtaag cccccttctg ttctatgacg gcattcagct tagcgccata
       61 cccgagcttg gctggacgtc catgttagca gctctatttt ggcttgcacc cacagggttt
      121 cgccgtttca tccgcattca gcgcgacctc agcctttcgg catcatagct ttaacgctgc
      181 gcggtatcgt ttctgtgcca gagccggagc tctcgcccca catcctttgg gatgtctgtg
      241 tgtccagtgg tggagggact ttccacaccg gccggagctg gccagcggca gccgtccttc
      301 ctctctcgca tcattcaaga tgccggttta ggtttataat ggcttcgctt gccggtcata
      361 aaccgccagg cttaacctgg cctggccaac cccatatgcc accctcgggc aagtctatat
      421 cctatgacag ccattctatc ccgcagatgg cccgagacca gaaggatcac tactaccgca
      481 aggcgaaaga ggaaggctac agggcccgat cggcctacaa gctcctgcag ataaatgaga
      541 agttccatgt cataaaaaaa ggcgactccg tggtcgacct gggggcggcg ccaggcggct
      601 ggctgcaggt agcccagaag ctctccggag gaaagatcgt gggagtggac ctggagggca
      661 tcacccccat cccaggagtg gtcaccttca gggcggacat caccgccctg agcacagtgg
      721 acctggtaaa agatgccctt ggtggggatg cagatgtggt gatatgcgac gccgccccca
      781 atctctccgg ggcctgggac agggaccatg ccatctccat agacctggcc cgttccgctc
      841 ttgagatggc aaaaaagctc ctccggccgc ggggcaactt cgtggtcaaa gtgtttcagg
      901 gagatatgtt catagatttt ctcaatgatg tcagacggga gtttgcagtt gttcatgccc
      961 attcgcctgc ggcctcaaga aaggagagcg cagagaccta tgtagtggga aagaagctcc
     1021 tttctgcccc ggtccgcaaa ggggacatgc tcaatgttcg gatagaatct gtgggcaaat
     1081 ctggagacgg cgtggccatg gtggagggct tcgcgattat tgtccgagga tcgaagctga
     1141 aagaagagct tcaagttaag gtggatgccg ttttgaccaa cttcgccttt gccgaaatcg
     1201 tagagagaaa gagttaataa cctgcccaag aaaagtgaac ggaggggcta gaccatggaa
     1261 gacgatgcag tgattttcat tggagacaaa ccagtcatga actacgtcct ggcagttatg
     1321 acccagttta acaagcctat tgccagtgtg atattgaaag caagagggcg tgccatctcc
     1381 cgcgctgtgg atacggcaga gataacaaga aacaggttca tgcccaatct cacagtcaag
     1441 aacataatca tcggcacgga atccattccc aacgaggaag gaaagatggt gaatgtctct
     1501 tcgatggaga taatcctcac aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nntgtgacag tattcagagc ttatgatgtc agaggtgtct acggcaccga tctcacagag
     1681 cagttggtga agagagttgg tgaatccttt ggatcttatg ctgaaggtgg ttcagtctcc
     1741 ctgggaagag atacccggat cagtggccca agcctccaga aggctttcct ggatggagta
     1801 ctgagcacag gctgccaggt taacagcttt ggcatcatcc ccattgccat aatcagcttt
     1861 atcacctgga aagaggagct caaggccgca gcctacatat ccgcctctca caatccgccg
     1921 gagtacaacg gcgtgcgatt cagaacctgt gacggttacg gcctgctcta tcaagagtcc
     1981 tccataatga agtactaccg ggaggggggc tttctcgctg gccagggggt caagaccgat
     2041 cgcgatccag gggaggcgat cgagagatac agggagtatg tgcaagagaa gctgaacctc
     2101 aagcgtcccc tcaagctggt tttagacatg gggaatggtg cagcctgcac aatggcctcc
     2161 ttctaccagc ggttgggttt tgatcctttg attttgaacg gaaccacaga cggcctgttt
     2221 cccggtcgag gtcccgctcc caatgaggaa tctttgcgcc ccgcctgcca gatgctaaag
     2281 aaaaaagagg cagatttcgg cgtgggattt gatccggatg ctgacagggg aatcgtcatc
     2341 gatgataaag gagctgttct gcccccggag aaggtggcga tcatcatcac ccgggcaagg
     2401 tacaagcccg gtgacctggt catatccgga ttcgactgtt caatgatcct ggagcgagag
     2461 ctggaaaagg atggcataaa ggttctgcga gagagggtgg gcgatgtctt cgtggccaac
     2521 cgggtgaaaa actcaaaagc ggttctgggc gtagaacgaa gcggccactt cttcctgccc
     2581 gagttccagt actctgatga tcccttcgcc atgagcctcg ccctggctga gatcgtgtcc
     2641 agtggagaga agctctcaga tctggcgggc atgatccctg attatcccta taaacagatg
     2701 agcatccacc tcaatgaaga cccggcagcg gttatgctcc gcctggccga ttcactggct
     2761 ttctttgagc cggacacaac agatggcttg aagatcacca ccgaaagcta cagcatactc
     2821 atcaggccga gcaacaccga gcccatcatc cggctgtata tcgagaccac caaaggggat
     2881 atgaaagagc tggaggagca atacaaaaag atcatccacc aggcgatgaa agcctgaaaa
     2941 atttcgccag agctgattta agcgtgagcc ataaagacat ctgcattctc ataccgaccc
     3001 tcaacgaaga gaaatccatc cagcccgtaa tccgcgaatt ccaggagctg ggctatgata
     3061 acatcctggt cgtggacggc catagcaccg acaagaccgt nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn aaaaggctga atatgttcgg caataagatg atcaaccgct
     3241 tctttgcggc aatctacggg atttctctcc acgatatcct gtccgggtac agagccttca
     3301 ccacggaagg aataaggatg ctcgatctat ccatgcctgg ctttgagatc gagtcggaga
     3361 tgaccattga gagcgtaaag aaaggcctga ggataatcga ggttcccatc acctaccagc
     3421 cccggtccgc cggcaccaag acaaagctgc atccgttccg ggacggtctt aagatcattc
     3481 tcaccatctt ccgcatggcc aagacggaaa accccatgtt ctactttggc ctgatgggct
     3541 cattcgtggg attgctcggc tttctggtag gggtctatgt ggccagagac tggttattgt
     3601 ggagaataga gcatattccc ttaaccatac tgacggcgat tctgatcata gtcggcatcc
     3661 aattgttcat gatcggcatg cagggagata tgatggcctc catgcaccgg gagatgatga
     3721 gagagctgta ccgaaaaaaa agataaggcg ttccgccaaa agctcttatt tagcggaact
     3781 cgagcttggc ctcgctcttc gcctcgaaga gatatgccct tcctggctcc agagtctcgt
     3841 tctctcctag attggtccag tccccgccct catgcttcca gacgaatgct gagaccaagc
     3901 ccttttctac tgcctccggg tacctgtacc tcttatgctc ggcattgatg gtgatgttag
     3961 ccagatcaac ggtctgattc accggcagtc cgatcatatt ccagcccaca tgcagatcca
     4021 gtcggtaggg gaggtcgacc ggctttcccg gtatctccat tgtgaagttc ccggtgctgt
     4081 ctatgagata tccctcacct gctttgaatt gagtcacatt gagcttgcct gcctctttca
     4141 tgccgaaatt ccatccatct tcggcaatgg tgaatatgct cctgtagggt ttgttcttca
     4201 ggtatctctc cgcagatgca tcccgcgggt tcagatagat ggacacagca ttccagccgg
     4261 cagtgacatt gatcctctga acctgaccgg gtataagctc gtactcgata ataaagctcc
     4321 tcttctcccc aagcttcggc ctgatgatgg gccacatcac cctcttctcc aaaccccggg
     4381 aatattcatc atgccagagg gacagcccat cctccttgac cacgatcttc tctgcgtcct
     4441 tgggcaagag cacctcacct atgatggcgg gagtggcatc caggttgaga tcgccactgg
     4501 agctcttcga ctcaatccag tatacccaat cggtagcgct ctcgttaagc ggaatcactg
     4561 ccacatttag aatcgtctga ttcaggtcgg tgtcgttctc cgccgagaga acgatggcat
     4621 agggaagaga aggagctttt ggcaaagcag gcttgaagct cggatcgccc aggattacgt
     4681 tcatgtaaga ggtctggttg agcatctcat gaacggagat atcccagtcg ggcaggacct
     4741 catcgtaatc tgagttcggc tttaggcttt cagtgcctct ggacttcagc cggaagaggt
     4801 tgtccgcatc cagctgggcc tgacccactg tggaattcct gaagaccagg gactgataga
     4861 agctcttgta gtagtcgtct gagagaaaga tgtaggatag ggctgagcct ccaatgtagt
     4921 ttaccgctcc cgcccttatg aatgccagag cgatggaatc ctccaggttt ccggggacat
     4981 acatcctcgt gccggtcaca ttcagatagt atcccttcag gttggtggta acacaggcag
     5041 aagcagtggt tgtctgagga ggcaaagtca gctcttttac ctgcgacccg gtaagagctg
     5101 aatccgtcat tgaccaggca gacagccccc atgaatcctc acttccgtga tgatcgaaat
     5161 gagcgatatt ctggccgttg ttcaattgcg atatcgtctg ctgataagta gcctcttcat
     5221 gacgcagatc cttaaccata atacctgcct cctccagata ctcctttatt cgtattccca
     5281 caggagcctg aggaaatgag agtgcgggag aggatacaac cagcccatta ttcttccagc
     5341 ttccatttag ccgatcataa gccattgtgc gggccatgag ctgggaggca tcgtaaaccg
     5401 atagccccat gatccttccc acagccaccg tgctgtagtt gttcaaatcc ggcttgatct
     5461 gatagtctct gtagacctca tactccataa cctccgacag cttctggatg agcccggtgg
     5521 atatgaaagg aatagagccg ggatcgccca ccaccatgag atactccgga tccaggttaa
     5581 gctccgctac cttttgattt agcgtctcct ccaccaccag gggatcgatc ttttggggat
     5641 cacaggccac gtcaatcaga atggctttat gagatgatgc cgcccccgcc gccaccatag
     5701 agagcctggg gaccttggta tggctgaatg ctgtgatatt caagaccttg acattggagc
     5761 gaacctcgat ggtgtaattt tcagtccggg cctcatccgt ctcgatattg aaaagccagt
     5821 tgcctggagt ttgaagggag actctcacat catagagccc atccctggtt atgtttgtca
     5881 gcttcaggcg gccagatctc tcatccgggc tgtaaaggtt taaagcgaga taactgctgt
     5941 tccgctcaat gttccaggag atattggtgc tgcctgagta gatccatttg ggataggtta
     6001 gattgattac aggcggatcg tccacatttc tgagagtatg gttgtaagag aagccttcat
     6061 aggcggcagt agctccagtg tccaggcgct gcgaccagcc ggcatccatg ggatatagca
     6121 gctctacctc aacattcagg ctggcggact ctccaggctc gagccggccg aaatcccagc
     6181 ggatcagaga tccattaaga aaagcggagg tctctgtagc tggctttctg ctgtaggggt
     6241 cggtcacatt gacctccccg tatgcggtgc tcgccagcct tatgagccgc cccatgggaa
     6301 agatatctcg tagctgcacc cccctgagca cctcatctcc actattggtg agggtaaaat
     6361 tgagcctgac cacctctcca tcttcctctg caggaacctg gtcaggggat agctccgttc
     6421 tcaccagcag atctccaacc aacgaacgct caaccgcctt gctcgacgcc ggcggcagga
     6481 gggatagatc atgggagttg gtcaagacaa ccattgatgg actctcgttc cttctttcca
     6541 tctcctcgac cagcagatca ccgctgccct cgataagggt gatattcatc tggctcaggt
     6601 tttgtctcac tctctcccct gtcgggcctg ccaagatcgc atcctttgcc cccagccgga
     6661 cgagagccga tcgggtctcc tccggcagcg cctcattcac aaacaacagc ggccagttca
     6721 gataggatgc aatagtggcc gcctggagag cagcagtata attctcaccc acaattactg
     6781 ctcctggaga gctcgcccag cggcgactta tctgctcact cagatcaaag ctgttgttga
     6841 cccttatgtt cgagtcgctc ctccaggaga tatttcccac tgcaagaacc tctccattaa
     6901 gctgctgcag cagccaactg gccgccgagg aggggggctc aagctcagac cagaggggtg
     6961 tatggaccac atcgtactcg aagacgactc ccgaggcacc tgatcccacc agatcctcga
     7021 tatagcttac agctcccttc acattctggg tggagaggac atagcccaca taatcatcat
     7081 taaggcgaac ataaacggga ttcgtgctct ctctgctggc cagcttgacg tcctggggag
     7141 agacgggaga tatcaatgca aagtctgcca cctcggtcat ccgggcaaag ttgtatccct
     7201 tgcttcggtc aaatgggctg tccagtgcca cgccgagcag cacagggccc aggtcagatg
     7261 tgatgttccg ggcatccata acgatatcca tgagctggtc ctgcttccat tcagcccatc
     7321 tctcccggtt gtagctattg gcattgacct tactgatatc tattcccgtg tcctgataga
     7381 acttctcttt gcatacatcg cagtagcagt gattctcatc ctggaatccg aaccgatata
     7441 ggacaactcc atcgacatga tagtcatcga tcagcatctc aatctggtta taaagacggt
     7501 cttttacctc tggattcacc gggcagtaat agctgctgtt tcccccgatc tctggatttg
     7561 gctcctgcag catagagcga tcgatggtgg actgatccgg ctctgttatg gcgaggccaa
     7621 cctcagccat caatgccatc tcgttactgt ccagaccttc cagcgcctcc gatgaagcct
     7681 gggatacagg aatctctaaa gtgacggcaa cataagcttt gattccgtcc ttttgagccg
     7741 ccttcaccag cgcaccgacc ttctcgccgg aaccatgtat ggttatagcg ctgatgtttg
     7801 cccctttgag ggtatccagg attgccgaag caccgtatcg ctccagatcg ctctcttcga
     7861 tccagccatt tctctctcca gcatgcacct ctttggggag gattagcata ggcttgatga
     7921 cggtctcgtt ctcctgagaa tagatggcga ggggagtagc agcaacagat gcatgccagt
     7981 cgtctgctcc caccaggatg atatcctcga cctcgccttt cactcctctc gccagtggtt
     8041 catcgtctgc ggcggacagc agagtggcgg acagtattgc cataaataat aataaataaa
     8101 aacgcttcaa gcgctcacct cctctaataa gacacccgct tccctaagtg cctttgtata
     8161 atcctcttcc actctgccca cgaccatcgc ccgagagagg gttgtattgc gtctggtcat
     8221 ctcctcaata gcttctatgg aggcaccagt gacgttgcct ctgcacacca ccatcggtaa
     8281 tccctcgttc atcgctatct tataggctct gaatatgtct gcttccctgg gtgtggtcaa
     8341 gacaacgttg gatgtgccat tatcccagat cagtttcgtg aacagccagc tctcttcaaa
     8401 gaggctatcg ctttgaatgc gcttgagttc aatatctttc agtttcttct caacatgaga
     8461 ggggatcaca gaaccgttgc ccaggatcac gatttcaaga tagctctgca gcctctgcag
     8521 gtaatcatcg gagatctcat ccgctggaac cataaatatg gatgggctcc tattgccaaa
     8581 taaggccaga gtaccaatag tgccatagat gatgtcatct ccggtgagca ccgccaatcc
     8641 ctctggaggc gcagccacaa tagtggcggt ctcctcaggc atgggaatgc cgtgggaatc
     8701 caatagctgg aaggtaagcg gatactcgcc cgcttttgat gccaggaagg tgaagttggc
     8761 caacccaggc tcctctggtg tgaccgttac catttgtgac tgggggatga ctataagctc
     8821 atccggagaa tgcatcgaga tccatatgtc ctctttgacg tccagagtta tcttgaattt
     8881 gccctcatag ctcttgttgg tggcgatctt ctccgggacc aggatatcag tatcgtaccc
     8941 tctgaccacc attccgaact cgacctctgc ctggattgag gagttcatgg accggttgcc
     9001 cagggatgtg gcagtgatgg tgacattctc ccgagatccg ggcttgtcgg tctgtatctg
     9061 cagcttgatc tcatgggttg ccccgggatt caggggcagg tcatagggca tgctcatggg
     9121 ctcgtcatcg tccatcagca cgacgctcca gccctcgggc atagctcccg cgtcaatcga
     9181 ggcgatatct ctgaatgagc ccagattctt gactgccaga ttgaactggg caatatcttt
     9241 agcgcttgcc cggagatagg ctgatttggc atcatccacg gtcatggcaa tgccgtagct
     9301 ctcctgcaca acgacattaa catgaaagtc tattcgcacc ggaatggagg gtggagcact
     9361 ctccatcaag tatacaaatc cggtatagac accgggctcg gtctccgggg gtataccgat
     9421 atagatcttg atctccgcct cctctccagg agaaagaggc ttatcatccg gaacgtcgat
     9481 aaagataaaa tccctggcat ttccgatcat ttcggtttta gcgatattga agatggttga
     9541 gtccccatta ttcatcacct taagaatcct ctcatctcca tcccctggat gaagaacgat
     9601 gtcaaatctt tccttatctg gtgccagctg ggcggcgccg gcgccgatca gcaggaacag
     9661 tattataatc gggtatatca gcctcatctt gccccacttg tcatatactg gatctttgca
     9721 cagtgaaata tcacatctgc tcgctttaaa tcctttgccc agaggattgc aggaattcat
     9781 t
//
LOCUS       DAOSGP010000022         9703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_5969_length_9703_cov_12.725850, whole genome shotgun sequence.
ACCESSION   DAOSGP010000022 DAOSGP010000000
VERSION     DAOSGP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5969_length_9703_cov_12.725850"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..604)
                     /locus_tag="PLV49_01360"
     CDS             complement(194..604)
                     /locus_tag="PLV49_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97516.1"
                     /translation="MKDRQSLTRKIVPRYQKARKAEKEKILDEFAKNTGYSRNYAIHI
                     LATWGEVHTLVIDEKPIRVVVGVSRTRKKRKENVHYTISPATIDRLLKVERKKLEIKG
                     RSHTKGGVLLKHQIPISTFYDWYERKPGFFTRGS"
     gene            872..2029
                     /locus_tag="PLV49_01365"
     CDS             872..2029
                     /locus_tag="PLV49_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG97517.1"
                     /translation="MRKLVLGLIIVNLLLGTVVFAQEPIKIGVLVEFTGACAAYGQMG
                     RDALELARAKGIVPVQVLGRPIELVYFDARTEPTEATNGATRLINTEKAVAIIGTMCS
                     GPYLAAAEICQASGVPIIGPTTTNPLCTQVGDYCFRACFTDNDQGPLAASIAQKRYKA
                     KTAAVVIDMAQAYCVGLGQFFMDAFKALGGKIVAELYCRSGDVDYTAQLTEIQRKNPD
                     IIYTPNYYTEVAMMARQARDLGMMQPILGADGLYAPELIKIGGDAVEGLNFLTFWSAE
                     VTPNEASKKFVKEFRAKFGMDPDAFGALTVDCYMMIVDAIKRAGSANPAAIKQALKTS
                     DLDVITGHVIMTPKNDPKKDFAFLTVKEGKFSLVETIPVEQAWEIRALIGK"
     gene            2093..2983
                     /locus_tag="PLV49_01370"
     CDS             2093..2983
                     /locus_tag="PLV49_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG97518.1"
                     /translation="MFYVQQLFNGITLGSLYALIAIGYTMVYGILRLINFAHGDIFMW
                     AGYWVFFSFFLFHLPWWIGAILAVVLTAGMGMAIERFAYRPLREAPRISLLITAIGAS
                     FLLENLIIVLPWAGGRQKSFPRPEFMSQIFSLGTLRIQALTVWIPLIGLACLIGLLFL
                     IYHTKIGRAMRAIATDIEATEIMGADINKIISYTFLVGSALAAVGGILWGCRYPRLQW
                     DLGITPGWRAFTAAVVGGIGNVTGAMIGGFALGLFEILLVAFIPNLSGYRDAFIFGIL
                     IIFLLVRPTGILGKPLKEKV"
     gene            2980..4017
                     /locus_tag="PLV49_01375"
     CDS             2980..4017
                     /locus_tag="PLV49_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG97519.1"
                     /translation="MKRYRNLVLTLIAIALVGVFVWWGDKPNNLDPLIWRILNLGAIY
                     AIAAVGYTVINGIAGQFSLGPHGFMLLGGYMVALLMLPLKQKAVVWLFKGKELIWPFS
                     SFTLPSWGFILALLAGGVLAAVAALIVGIPALKALRGDYLAIATFGFGEILFVLACNL
                     IPLTNGPMGIKGIPHITNLAWSFGALVVVTYAALRIKNSSYGRALRAIREDEIAAESM
                     GINVFRLKMLAFIFSAFCAGVAGGLMGTLIGTVTPDTFKFFMTFYLLIIIVLGGLGSI
                     TGAVVTGFIFAGLFEALRVLDTATVEGMRMVFFAILLIGLMLFFRRGLFGEAEFSWDW
                     LFRGSSKLGRK"
     gene            4059..4811
                     /locus_tag="PLV49_01380"
     CDS             4059..4811
                     /locus_tag="PLV49_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG97520.1"
                     /translation="MRFGGLLAVNDFSLTITSGELLGLIGPNGAGKTTIFNMITGYYP
                     PTQGQVIFEGQDITGFPPAKVTLLGIARTFQNIRMCKNMSALDNVLISFHPHLRSSVF
                     GVIFPFFGYNREEQKMRQEGMDLLARVGLTQWANERAGSLPYGLQRQVEIARALATHP
                     KLLLLDEPAAGMNPKEAGGLMEFICQIQEEFNLTVFLIEHVMQVVMGICPRIRVIDFG
                     VSIAEGCPDEIKANKKVIEAYLGEEELSAGNT"
     gene            4795..5502
                     /locus_tag="PLV49_01385"
     CDS             4795..5502
                     /locus_tag="PLV49_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG97521.1"
                     /translation="MLEIRDLSVYYGGIHALQGISLDVPDGVVVTMLGANGAGKTSTL
                     RTILGLVHPKEGTIKFNGTSLLGKRPYQIVKMGVAMAPEGRRIFPDLSVWENLLMGAY
                     SRTDAEGIKKDLEWVLGLFPRLAERKSQAGGTLSGGEQQMLAVARAVMAHPKLLLLDE
                     PSLGLAPVLITELYRAFAKIHKEGRTILLVEQNARAALSLANYGYVLETGRIVAQGSR
                     EELLCSDLVKRAYLGIA"
     gene            complement(5731..8319)
                     /locus_tag="PLV49_01390"
     CDS             complement(5731..8319)
                     /locus_tag="PLV49_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOG97522.1"
                     /translation="MEAKKKTHPKASVVVAKKPAARRGSSTSIKASARSSALRSTRGI
                     IALIFFIVALFLFISMLLWSYFPTIWRPLVLPGQTLLNIFSWAAYYLPLWLAWAAILV
                     YVPSFCPRMTYLLGTSALPFVVATGFARLSANPGSFFEHHPSMAKVGLSSLYAALGFL
                     LAASLMIVIAGYIKLSEWLRANGFIKTRADKKKNGEEQSFGQRLATFIKDNSLKREER
                     RKRKEEARLARARALAEKDISLNIPDVPPPDPLKSEKLGGFYASGTSRAGSEMAQKDL
                     GATSVPMGSAIGSTGLSLGSRSLGPIAGFDTGGISFTSVPPARPAQSEEEHKRAIVER
                     ILERKAAKPYQVPIDGLLNNYPDGQYWIIDEKTRASASVLKETLAEFGIEAEVTGIRK
                     GPVITMFEILPAHGVKISKITNLSDNIALRLAASSVRIVAPIPGKHAVGIEVPNERRA
                     IVSLRELIESEKFRQSKMEIPVALGKDIAGEVQLIDLTQMPHILIAGATGSGKSVCVN
                     SIILSILYKRTPEEVRLMLIDPKIVELKLYNDIPHLLTPVVTEAKKAFQALQYCICEM
                     ERRYSLLDNLGVRDIRSYNRRIRERNLAQDKVPYIVVIIDEFADLMQTTGKELEATLA
                     RLAAMSRAVGIHLVLATQRPSIDVITGLIKANIPSRIAFMVASKFDSRTIIDMVGAEK
                     LLGRGDMLFSGAQDPFPVRMQGAFVSEEEVERVVAHVKTLGEPEYIDEEIFVDEEESE
                     EPTLFDEEGSDPLFEKALEIVLQQGKASVSYIQRRLKIGFNRAARLVEMMEEKGIVGP
                     AQGSKPRDVLRNPGVFESSSGYEGIRKDASPDIEQIAGTSGSPSINPSLNPSPRRTFR
                     QDDQDN"
     gene            8538..9056
                     /locus_tag="PLV49_01395"
     CDS             8538..9056
                     /locus_tag="PLV49_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG97523.1"
                     /translation="MEIRKDRVVTIDYSLSDDIGKIIDSSEGSEPLVYLHGNDNIIPG
                     LERELEGKIPGESITCSILPADAYGDRNESLVFKVNKKDFGDNVEVDAGMQFEAHGED
                     GTLIVTVVNVNGEEVTLDANHPLAGETLHFDVKVIDVREATPEELEHGHAHGEDEDEC
                     GDDCCCEDGFCD"
     gene            9481..>9703
                     /locus_tag="PLV49_01400"
     CDS             9481..>9703
                     /locus_tag="PLV49_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97524.1"
                     /translation="MRARTSSEEPPVLDAPAFLEEPAALKAPPVLEALAVLEAPPDFS
                     LKQAPSIGQTSLGHENQAELTALLAAFCFF"
BASE COUNT         2284 a         2357 c         2471 g         2591 t
ORIGIN      
        1 actttaagta atcaaatatg aattctcaga atgttctaaa agactcctac atgaagaaga
       61 cgtatattta ttttgggaga gattcataag cttcgtgact gcatcatcga gtagatgttt
      121 gagctcgatt ggtggaggcc tgctgccctc tggcgtagat ttgtcttcgc cttctcatca
      181 agattaggac tttctaagag cctctggtaa agaagccagg ctttcgctca taccagtcat
      241 aaaacgtact gatgggaatc tgatgcttaa ggagtactcc tccttttgta tgcgacctac
      301 ctttgatttc gagtttcttt cgttcaactt tcaagagccg atcaatcgtt gctggactta
      361 tcgtatagtg cacattttcc ttgcgctttt tccgtgtccg agaaactcct accaccactc
      421 gtattggctt ttcatcgatc acaagagtat gtacttcccc ccatgtcgca aggatatgaa
      481 tggcatagtt gcgactatag ccagtattct ttgcaaactc atctaaaatc ttttcttttt
      541 ctgctttcct tgctttttga tagcgcggta cgattttcct agtgagtgat tgcctatcct
      601 tcattcgtaa gcccattgct gtgcctccgc cttgtatttt ttgctaaggc gtcacactct
      661 gccagactta gaatttatga tgaggaatta atttcatttc agctaaattt ttgatgagca
      721 atacattgtc ttgctaactt gagcggcaag atgtataatt aattttcttt ttcaggcctt
      781 caaaggaggt gatgtgcctg agaaggagat gggcaaagtt cttcgaaggc ttttgcggta
      841 atttcggtat aagatttaag gaggagaaat catgcgaaaa cttgtgttag gattgattat
      901 cgtgaatttg ctcttgggaa ccgtagtctt cgctcaagag ccaatcaaga ttggggtgct
      961 tgtggagttc accggtgcat gtgctgccta tggtcagatg ggacgggatg cattggagct
     1021 cgctcgagcg aaggggattg tccccgtgca ggtgctcggc cggcccattg agcttgtcta
     1081 tttcgatgcc cggaccgagc ccacagaagc gaccaacggc gccacccgtc taatcaacac
     1141 agaaaaggca gtggctatca tcggaaccat gtgctctggg ccttaccttg ctgcagcgga
     1201 gatctgccag gcatccgggg tacctatcat tggccctacc accaccaacc ccttgtgcac
     1261 tcaggtgggg gattactgtt tccgagcttg cttcaccgac aacgaccaag gtccattagc
     1321 tgccagcatc gcacagaaga gatataaggc caagacggca gcggtggtta tcgatatggc
     1381 tcaagcctac tgcgtcggcc ttgggcagtt cttcatggat gcgtttaagg ctttaggtgg
     1441 caagattgta gcagagctct actgccgttc gggagatgtc gactacacgg cccagctcac
     1501 cgagatccag aggaaaaacc ctgatatcat ctacacgcct aactactata ctgaggtagc
     1561 gatgatggcc cgccaagcac gagacctggg catgatgcaa cccatcctcg gcgctgatgg
     1621 tctctatgct cctgaattga tcaagatcgg tggggatgcc gtagagggct tgaatttcct
     1681 cacgttttgg agtgcagaag ttacaccgaa tgaggccagc aagaagttcg tcaaagaatt
     1741 ccgggccaaa tttggcatgg atcctgatgc ctttggtgcc ctgaccgtcg actgttatat
     1801 gatgatcgtg gacgccatca agagggctgg ctcagctaat cctgcagcca taaagcaagc
     1861 tctcaagacc tctgacctcg atgtgataac cgggcatgta atcatgaccc ctaaaaatga
     1921 cccaaagaag gacttcgcct ttctaacggt caaagaaggc aagttctcct tggtggagac
     1981 gatccccgta gaacaagcct gggaaatcag agccctaata ggtaaataat atttcctgag
     2041 gtgggccaaa gttgactact tggctcaccc cctattcctg ggaggcacag tggtgttcta
     2101 cgtacagcaa ctgttcaatg gaatcacact ggggagcctg tatgccctta ttgctatcgg
     2161 ttataccatg gtgtacggga tcctcaggtt aattaacttt gctcatggcg atatctttat
     2221 gtgggcaggt tattgggtat tctttagctt ttttttgttt cacctgccct ggtggatcgg
     2281 ggctatcctc gcggtggttc ttaccgctgg gatggggatg gcgatcgagc gattcgccta
     2341 ccggcctctt agggaggctc cccgaatctc cctcctcatc accgcaattg gagcatcgtt
     2401 cctcctggaa aacctgatca ttgtacttcc atgggcaggc ggacgacaaa agtcattccc
     2461 caggccggaa ttcatgagcc aaattttctc tttgggcaca ttgcgtattc aagccctcac
     2521 ggtttggatc cccctgatcg gcttagcctg tctgattggg ctcctcttcc tcatctatca
     2581 cacaaagatt ggccgtgcga tgagggctat cgctaccgac attgaggcaa cggagatcat
     2641 gggggcggac atcaacaaaa ttatctctta caccttcctt gtgggatcgg ccttggccgc
     2701 ggtggggggc atcctttggg ggtgccgata tcccagattg caatgggatt taggaatcac
     2761 tcccggctgg cgagcgttca ccgctgctgt ggtgggcggg atcggcaatg taaccggagc
     2821 catgatcgga ggtttcgccc ttggactttt tgaaatccta ctcgtggctt ttattcctaa
     2881 cctttctgga tatcgcgatg ctttcatctt cggtatcctg atcatattcc tcttggtacg
     2941 gcccacaggg attctgggca agccgcttaa ggagaaggta tgaagcgcta ccgaaatttg
     3001 gtgctgaccc taattgctat tgccctggtg ggagttttcg tctggtgggg agacaaaccg
     3061 aacaatcttg atcccttaat atggcggatc ctaaaccttg gcgccatcta cgccatcgct
     3121 gcagtgggat ataccgtgat aaatgggatt gccggtcagt tctccttggg cccacacggg
     3181 ttcatgctgc taggcgggta catggttgca ttgttgatgc ttccgttgaa acaaaaagca
     3241 gtggtatggc tattcaaagg aaaagagctc atctggccat tctccagctt cactttgcct
     3301 tcttgggggt tcatcctagc cctgcttgct ggcggagtat tggcggctgt ggctgcgctt
     3361 atcgttggca tcccggccct caaggccctt agaggagatt atcttgccat cgccaccttc
     3421 ggttttggcg aaatcctgtt tgtccttgcc tgtaacctca tacccttgac caacggtcct
     3481 atggggatca agggcatccc ccacattact aatctggcat ggtcatttgg ggcgctggtt
     3541 gtggtgacat acgcagcctt gcgtatcaaa aattctagct acgggagagc gctacgcgcc
     3601 attcgagagg acgaaattgc cgccgagtct atggggatca atgtatttcg cctcaagatg
     3661 ttggcattca tcttttctgc cttctgtgcc ggagtggcgg gaggattgat gggcaccctc
     3721 atcggaactg tgactcctga cacatttaag ttcttcatga ccttttacct actcattatc
     3781 attgtgcttg gtgggttggg gagcattacc ggggcggtgg tgactgggtt tatcttcgcc
     3841 gggctatttg aggcgttgcg ggtcttggat accgccacgg tcgagggtat gcgcatggtg
     3901 ttctttgcca tcctcctgat tgggctcatg ctcttcttcc ggcgggggct gtttggcgaa
     3961 gcggaattct cctgggattg gttgttccga ggttctagca agctagggag gaaataatgg
     4021 aaaaaggcaa gccgatcctc aatcttgatc atctcagtat gcggtttggt ggcctgttag
     4081 cggtaaacga cttttccctg accataactt ccggggagct acttgggctc attggtccaa
     4141 acggtgccgg caaaaccacc atattcaaca tgatcactgg ctattaccca cctacccaag
     4201 gccaggtgat cttcgagggc caagacataa ccggttttcc cccggccaag gtcaccttgc
     4261 ttggcattgc cagaaccttt cagaacattc gtatgtgtaa gaacatgtca gcgctggata
     4321 acgtgcttat atcattccat cctcatttgc gctcctcagt gttcggggtc atatttccat
     4381 tctttggtta caaccgtgag gagcaaaaaa tgcgccagga aggaatggac ctccttgccc
     4441 gggtggggct aacacaatgg gcgaatgagc gggctgggtc tctcccctat ggtctacagc
     4501 gacaagtgga gatcgcccgg gcactggcca cacatcccaa attgctcctc ttggatgagc
     4561 cggcagcggg aatgaatccc aaagaagccg gagggcttat ggaattcatc tgccagattc
     4621 aggaggaatt taatctcact gtctttctca tcgagcatgt gatgcaagtg gtgatgggaa
     4681 tctgcccccg catacgagtt attgacttcg gggtctccat tgccgaagga tgcccagacg
     4741 agataaaggc caacaaaaag gtaattgagg cttaccttgg ggaggaggag ctaagtgctg
     4801 gaaatacgtg acctttccgt ttattacggc gggattcacg ccctacaggg catttcactg
     4861 gacgtgcctg atggggtagt ggtgaccatg cttggggcca atggtgctgg taagaccagt
     4921 accctgcgaa cgatccttgg cctggtgcat ccgaaggagg gcacaataaa gttcaatggg
     4981 acctcactcc ttggcaagcg cccgtaccag atcgtgaaga tgggggtagc catggccccg
     5041 gaaggacgac ggattttccc tgatctctca gtgtgggaga acctgctcat gggggcctac
     5101 tcccgaactg atgcggaggg aattaagaaa gatctggaat gggtgttagg gcttttcccc
     5161 cgactcgctg agcgaaagag ccaagctgga gggactttat ctggcggcga gcaacagatg
     5221 cttgcagtgg cgcgggctgt catggcccac cccaaacttc tcctcttgga tgagccctct
     5281 ctaggcctgg ctccggtcct gatcacagaa ctctaccgag catttgcgaa gatccacaag
     5341 gaaggtcgga ccatcctgct cgtggagcag aatgctcggg cagccctttc tcttgccaat
     5401 tatggctatg tgctcgagac agggaggatc gtggcccagg gttcccggga ggagctcctc
     5461 tgttcagatc tggtaaaacg cgcatacttg ggcatcgcct agtatactat cccagaaata
     5521 cgctcgcata aacgagtgat tttctcaaaa ccgactctga tcctgcaaag tgacgagcaa
     5581 actttccctg ttctatctca aaaggccata tagttggcga taggtttatt ttggttgcaa
     5641 aaatgcgata acttcattgt gaatcgatct atcatcttgc tctgcatgcc aattccacgc
     5701 gtcgcgcgct acctgtatcg tgtcttatcc ttagttatcc tgatcatcct gtctgaatgt
     5761 gcgccgtggg cttgggttta ggcttgggtt tatgctcgga ctcccacttg tgccggcaat
     5821 ttgctctata tctgggcttg catccttgcg tatcccttca tatccgcttg agctttcgaa
     5881 cactccagga tttcgaagta catcgcgagg tttggagccc tgtgcggggc caacaatacc
     5941 cttttcttcc atcatttcaa cgagccgagc tgcacgatta aagccgatct ttagccgtcg
     6001 ctgaatatag ctaactgaag ctttaccctg ctgaagaaca atctcgagtg ctttttcgaa
     6061 aagtggatcg gaaccctctt catcaaagag agtgggttct tcggattcct cttcatcgac
     6121 gaaaatttct tcgtcgatat attctggctc cccaagagtc ttaacgtgag caacaactcg
     6181 ctcgacttct tcttcggaga cgaatgcgcc ttgcattcga acaggaaatg gatcctgggc
     6241 gcctgagaag agcatatcgc ccctaccaag gagtttttct gccccaacca tatcgatgat
     6301 agtgcggcta tcgaatttgc ttgcgaccat gaaggcgatg cgtgatggga tattggcttt
     6361 tatgagacca gttatgacat cgatactggg tctctgagta gctagtacaa ggtggattcc
     6421 aacagcgcga gacatggcag caagacgagc aagcgtggct tcgagctctt tgcccgttgt
     6481 ctgcatcaag tcagcaaact cgtcgatgat tactacgatg tagggcactt tgtcctgggc
     6541 cagattgcgc tcacgaattc ggcggttata agagcggatg tctcgtacac ccaggttgtc
     6601 aagcagagaa tagcgccgtt ccatctcgca aatgcagtac tgcagagcct gaaaggcttt
     6661 ttttgcttcc gtgacaactg gtgtcaagag atgtggaata tcgttgtaaa gctttagttc
     6721 gacgattttt ggatcgatga gcataagcct tacttcttca ggcgtgcgtt tatatagaat
     6781 tgataagata atcgagttta cacagacaga tttgcccgag cccgtggcgc ccgcgatcaa
     6841 aatatgaggc atctgtgtaa ggtcgataag ctgaacttct ccagctatat ccttgcccag
     6901 tgcgaccggt atttccattt tgctctggcg gaatttctcg ctctcgatga gctcccggag
     6961 agagactatt gcacgccgct cgttgggcac ctcgattcct acagcatgct tgcctggaat
     7021 aggcgcgacg atacgtacgc ttgaggctgc aagtctcaag gcaatattgt cgctcaaatt
     7081 ggtaatcttg gaaatcttga cgccatgcgc tgggagaatc tcgaacattg taataacagg
     7141 tcctttgcga atgcctgtca cttcggcttc tattccaaac tcggcgagag tctctttgag
     7201 cacactagca cttgcacgtg tcttttcgtc gatgatccag tactgcccat cgggatagtt
     7261 attgagaagt ccatcgattg gtacttgata tggtttcgct gccttacgct ctagtattcg
     7321 ctccacgata gcgcgcttgt gttcctcctc ggattgcgca ggacgagctg gagggacgga
     7381 ggtaaaagaa atgcctcctg tgtcgaaacc agcaatagga cctaaggagc gtgatcctaa
     7441 agataatcct gtggatccaa tagcggatcc catggggaca gaggttgcac ctaagtcttt
     7501 ctgcgccatt tcagagcctg ccctacttgt gcccgaagca tagaatcctc caagcttttc
     7561 ggattttaga gggtctggcg gtggcacatc aggaatattg agcgagatat ctttttctgc
     7621 cagcgccctt gcgcgcgcga ggcgcgcttc ttctttgcgc ttgcgtcgct cttctctttt
     7681 tagcgaatta tccttgataa aagtcgccag tctttgccca aagctctgct cttctccgtt
     7741 tttcttcttg tcggcgcgtg ttttgataaa tccattagct ctaagccatt ccgagagctt
     7801 aatgtagcca gcaatgacaa tcatcaggct tgccgcaagt aagaacccta gagctgcata
     7861 tagcgaagac aatcctactt ttgccatcga cggatgatgt tcaaaaaaag aaccgggatt
     7921 cgcagacaga cgagcgaatc cggttgccac tacaaatggc aatgccgaag tgcctaatag
     7981 ataggtcatt cgtggacaga agcttggcac atatacaagg atcgcagccc aagctaacca
     8041 taatggcaga tagtaggctg cccaagaaaa tatgttgagc aatgtctgcc ccggtaagac
     8101 caaaggcctc caaattgtcg gaaaatagga ccatagaagc atagaaataa acaggaaaag
     8161 cgctacaatg aaaaatatga gcgctattat tcctcgagtg ctcctaagag cagaacttct
     8221 tgcagaagct tttatcgagg ttgatgaacc acggcgtgca gcaggtttct tggccacgac
     8281 aaccgaggct tttgggtgag tcttcttttt tgcttccatg ggcagataaa tccttaatta
     8341 ttatcggcat tttggaatgt gccagttacg aatatttcga tgccttgctc cattgccgcg
     8401 caatataata attcattata ctatagccct gcctagagca tgaccgtcct tatgtatcga
     8461 ttgtacgcga ttcgtgctca aaggaagcgc attctaaatg cgatgaaagt attttgacta
     8521 ttttaaaagg acctgtaatg gaaatcagaa aagatcgcgt cgttactatt gactattctc
     8581 tcagcgacga tatcggcaag atcatagact catctgaggg aagcgaaccc cttgtgtatc
     8641 ttcatggcaa tgataacatt attccaggcc tggagcggga actcgaagga aagattccag
     8701 gtgagagtat tacctgcagt attcttccag ccgatgccta tggagaccgc aacgaatctc
     8761 tagtattcaa ggtcaataag aaggattttg gcgataatgt cgaggtagac gctggtatgc
     8821 aattcgaggc acatggtgag gatggcacat taatcgtaac ggtcgtaaac gtgaatggag
     8881 aagaagttac acttgatgcc aatcaccccc tagccggcga gactcttcac ttcgatgtga
     8941 aagtcatcga tgttcgtgaa gcaacgccgg aggaactgga gcatggccat gcacacggag
     9001 aagacgagga cgaatgcgga gatgattgct gctgcgagga cggtttctgc gattagtctg
     9061 agtttaggcc ttctctattc ttcaataatt gatcagcatt catgaaatca acctcaagag
     9121 tgcatgttcg atttctggat atccttatct ttgtactagc tatattgcta gttgttggaa
     9181 cagctatcct cgtgctcgat cggcagagtg gggcacgcta tgtcgaaata acaggagaaa
     9241 agggcgaatg gatagcccct ctcgataaag aagcagacta tgaaataaca ggccctttgg
     9301 gcatgactca tgtacacatt cataacggaa aggcaagcat taccgaatca ccttgcagca
     9361 acaagctctg tgtactggct ggctctattt ctgagccaaa tcaatggata gcctgtctcc
     9421 ctaacaaggt attcgtgcgt ataataagca atggcaacag caaggatgtc ggaggtgtgg
     9481 atgcgagcac gtactagctc agaagagccc cctgtcctgg atgcaccagc tttcctggaa
     9541 gagcccgctg ccctaaaagc gcctcctgtt ctggaagcgc tagctgtcct ggaagcgcct
     9601 cctgacttct ctttaaaaca ggcgccttcc ataggacaga catccctggg ccatgaaaat
     9661 caagctgaac taacggcctt gcttgctgcc ttctgctttt tct
//
LOCUS       DAOSGP010000023         9314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_6305_length_9314_cov_19.374230, whole genome shotgun sequence.
ACCESSION   DAOSGP010000023 DAOSGP010000000
VERSION     DAOSGP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9314
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6305_length_9314_cov_19.374230"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLV49_01405"
     CDS             <1..690
                     /locus_tag="PLV49_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOG97525.1"
                     /translation="AYRLSFATDPKSAFGGVIGFNRMVDVKTAQCIGDSFVEVLLAPG
                     YEAEALRLLTEKKPNRRILDIGDLLQRKSELYRGYYKKTIFGGMMLQDFDTGDIKEWR
                     VVTRRAPTDAEARALRFAWKVAKYVKSNALVYTTENQTIGIGSGQVSRVDSARFGAEK
                     AEEHGLSTNGTVAATDSFFPFRDGLDQAFLAGATAVIHPGGSIRDSEVIDAANEHDMA
                     MVFTGMRHFRH"
     gene            734..2155
                     /locus_tag="PLV49_01410"
     CDS             734..2155
                     /locus_tag="PLV49_01410"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HOG97526.1"
                     /translation="MLFYSLLFSPASADRIQAGQDIQSAINSAQAGDIIAVGPGTYSS
                     ITIDRPLTLIGEGRPVLNATQQNPAMVVKSDGVTIDGFEIYGVGKDTTAKFEYYMENR
                     EAARGQRLDMPNSAVVVEGSDLAIKNSSIYGAQAGIYALNAVGITLQDSTLNGCDTGL
                     FLHGGRGLNVSGCSIVNCKKYGLDIEQSRDIAVKNNSIIRNSNIGILLREGKWAEMDD
                     NLISECTFGLSLWNASFNQVRRNQLDHNYYGILVTNWSCYNNITDNLLIDNSRGEIID
                     GFGIGLSLQENSCHNMVIGNVARGNYNGLEISKGCQFNAVYANNAAENKHGIRMNENR
                     NNLIFGNNFQFNNINAYENQSCNIWNTTLGNYYSDYKGEDENKDGIGDVPYSLPGPES
                     SSSDRMPSIRPFDEGRLERADIGELVKKYALFEPKEEAGTAARLVEGVMVISHPLPSS
                     PPQWKDSEPLDVSSSPFQEVNDF"
     gene            2156..3217
                     /locus_tag="PLV49_01415"
     CDS             2156..3217
                     /locus_tag="PLV49_01415"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004809 - tRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HOG97527.1"
                     /translation="MTLHTEGSITFEATGAFFNPRMQLNRDIGVAMTKALGISEYLDA
                     LSASGIRGMRVAAEAGTEKVTLNDFDPRAVQVMRRNVARNQLDCIVSEKNANVLMHQE
                     HFQAVDLDPFGSPSPYISAASRSARSYLFITATDTAPLCGAHLKSGIRKYMAVPLCTD
                     YHREMGARILLGLAARELARLDKGTEPLLTHVTDHYVRTYLRVVKGAEAADRSLQSLG
                     YLEHCNSCGSFETLRLLHPVGICRHCRSRTALAGPLWLGKIQDPDVIANALEAGDLGP
                     RAKKILSTCAKEVNVPMYYDHHSICEKLRITPGKIEDVISRLMSLGHQASRTHFTGLG
                     IKTNATLPELEEVLRENNI"
     gene            3537..4208
                     /gene="npdG"
                     /locus_tag="PLV49_01420"
     CDS             3537..4208
                     /gene="npdG"
                     /locus_tag="PLV49_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HOG97528.1"
                     /translation="MKIALVGGTGDIGMGFALRLMCAHEIIIGSRKEDKARESASAIM
                     QLEGANGNTWGTDNASAVAVADAVILCVPPEHLKSVTYDLSTSFNHQLVISPVVPMSY
                     DGKFFRFNPPPEGSSALQAKSLLPPQIRVVAAFHTVPAASLQASDRILKGDVLICGDD
                     AEAVDMVKSLTLEIKNLRPLFVGPLAASSQVESLTPMLLNVARKNKIKEAGVSIISER
                     PPAPN"
     gene            complement(4213..5502)
                     /locus_tag="PLV49_01425"
     CDS             complement(4213..5502)
                     /locus_tag="PLV49_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOG97529.1"
                     /translation="MDFDKKEAKEDCPEPTCRIIEPDAGADLTEGFSHEITIVGTAHV
                     SEKSALEVVRRIEETRPDIVAVELCLSRYKALTGQEEKSEIEIGKLLSGGKLYVFLVQ
                     LLLAYMQQKIGQEMGVKPGSEMLAAIRAAQSTGARVALVDRDVGITIQRFWSAMGFFD
                     KIRLIGSLIQGALWGEEEEIDIDNITQDDVVSQMIGEFRKISPGAASVLVDERDAYLA
                     RNLLSLSRQGRVLAVVGAGHKEGIERHLRNPQAIPAIESLNVKAKKKITFSRVFGAVV
                     TLTILVMIALLVITAQSSRSLVLAFAIWFVITGTLSALGVILARGHPLSVLTAFGIAW
                     MTTLNPFMAAGWFAGMVEAWRIKPTVSDLKELPQAESYRQMMNNRLFKVLLVASLSNL
                     GAMAGTFIGVYVIWKVMDLINPTELPSMVIEALSGMI"
     gene            complement(5516..6616)
                     /locus_tag="PLV49_01430"
     CDS             complement(5516..6616)
                     /locus_tag="PLV49_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG97530.1"
                     /translation="MMNSSLQLGKIMGIPVQLHWSFLLVILYIAWAFASFSQQVLGRS
                     YGFGEIEPAGLRWAYSLIFSLVLFTCVALHELGHSYIALKNGIAIRSITLYFFGGVSA
                     MEEIPRNPRLELQMAFAGPAVSGILGLMGILLATLLDEGSPLGIMLWMLGLLNIILML
                     FNLLPAFPMDGGRLLRAWFATQMPYIKATQRAASIGKLFAIIMFVLGLFSFNFILLFV
                     AFFVYVGASEEEKATQISVSLEGTRVMDIMSDDVHTVDPDMTLQDLKEHMFEEKHRGY
                     PVVSGDEVIGVVTLSDLQRVPQVDHADTRVAEVMTRKLYVIGPSEEASAAMMMMNKMN
                     IRRLPVMSDGRLVGIVSREDLVRAIELCSGQD"
     gene            complement(6760..7365)
                     /locus_tag="PLV49_01435"
     CDS             complement(6760..7365)
                     /locus_tag="PLV49_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HOG97531.1"
                     /translation="MTSDAWQEIARFGRKVVSSGLTSSRFGNISICQDGRIFITATGS
                     MLDELDSSTVVEVSLENPCEMDKIASIETCVHRAIYLNTGKMAIIHTHSPYAVVLSVV
                     EMEKVEPIDSEGVLFLGEVPIIEGKLGTDMLAKAASLALKSHMACIARGHGVFAAGGT
                     LMEAYTAACMVEHSSLVRYLVNCYPQGKNLYLNQHTFKSSG"
     gene            complement(7370..9250)
                     /locus_tag="PLV49_01440"
     CDS             complement(7370..9250)
                     /locus_tag="PLV49_01440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOG97532.1"
                     /translation="MNPSHILHFVLLLSLVSYASAFPIQNALENNKATAWFQKEYENR
                     LRPSVDDGAAPDIYGVSVSPSILKTGDPRSEINARVHDPSGLGMVYADIGNRMNLMID
                     LDRDGTFTGYCGSNMPAGTYNVTIIAVDKFGNAAKDESKKITIRDPRDLNGNGIEDSL
                     EEQKAKDLKVIVLHEDDLKGNLTGVGSSLEKRFKVLPGSAMTVSSDNLEKIAKTDGVR
                     GVYTDQKLTVLSAASTSSSNPGPSIIDDPREISGPKGEGVTVALIDTGADADHEALSR
                     KIVAFQDFVNNQTEPYDDNGHGTHCASLIAGDLGMGVAPGAELVVIKVMDRDGACYLS
                     DALKALDWCLDNKDRYGIKIISFSVGGEGPSDGASLLDEACDRMVEEGLTMCVAAGNS
                     GPSPSSIVMPGGAEKVITIGAIDRTGLIFEQSSRGPTLTGEIKPDLVTLGVDVPSALA
                     GSKNGLSSVSGTSMAVPQVSGASAVLLEANPELQPADIKRLLLKTADELGQEGKDNLY
                     GYGALNLTRALKSINAPKEELYPPDLVDVRLNRDEATEGEPVMVEAQASGEIKSLNTK
                     IIGPDRNLEIPMDDLDGNGIYSARWETSFWTAGDYKISVDLVGGFGEMESIAVPFRLL
                     ER"
BASE COUNT         2163 a         2585 c         2437 g         2129 t
ORIGIN      
        1 gcctaccgac tctcctttgc caccgatccc aagtctgcat tcggtggggt gataggtttc
       61 aacaggatgg tggacgtgaa gacggcccag tgcatagggg acagcttcgt tgaggttctt
      121 ctcgctccgg gttacgaggc agaggccttg cggctcctca cggagaagaa gcccaaccgc
      181 aggatcctgg acataggaga cctgctgcaa agaaagagcg aactgtaccg gggatactac
      241 aaaaagacca tcttcggggg tatgatgctg caggattttg ataccggcga catcaaggag
      301 tggagggtgg taaccagaag agcccccaca gacgctgagg cccgggccct ccgttttgcc
      361 tggaaggtag ccaagtatgt caagtccaat gccctggtct acaccactga gaatcagacc
      421 atcggcatag gatccggcca ggttagccgg gtggactccg cccgctttgg ggcagagaag
      481 gcggaggagc acggcctgtc cacaaacggc actgttgctg ccacagattc cttcttcccc
      541 ttccgggacg gcctggatca ggccttcctg gcaggagcga cagcagtgat tcatcccggc
      601 ggatccattc gggactctga ggtgattgat gccgccaatg agcatgatat ggccatggtc
      661 ttcacgggta tgcgccattt cagacattag aatctgcaaa gggaggaagg cgtggactgg
      721 ccttcttgct ctggtgctgt tctattctct cctgttcagc ccagcctctg ccgatcgtat
      781 tcaagctggc caggatatcc agtcggcgat aaactccgct caggcggggg atatcatagc
      841 agtcggcccg gggacatata gttccatcac catagacagg ccattgacct tgatcggcga
      901 gggcagaccg gtgttgaacg ctacccagca gaatccggcg atggtggtga agagcgatgg
      961 ggtgacaatc gatggcttcg agatctacgg agtgggaaag gacaccaccg ccaagttcga
     1021 atactatatg gaaaaccgcg aggctgccag gggccagcgc ctggatatgc ccaactcggc
     1081 agtagtggtg gagggcagcg acctcgctat taaaaacagc agcatttacg gagcccaggc
     1141 cgggatttat gccctgaatg cagtggggat taccctccag gattccacat tgaatggctg
     1201 cgatacgggc ctattccttc atggaggaag gggccttaat gtctcagggt gcagcatagt
     1261 gaactgcaaa aaatatgggc tggatataga gcagtcaaga gatattgcgg taaagaacaa
     1321 cagcatcata aggaactcaa atattggcat cctcttaaga gaggggaaat gggcagagat
     1381 ggatgataat ctgatatcgg agtgcacctt cggcctttcc ctgtggaatg cctcattcaa
     1441 tcaggtgagg agaaaccagc tcgaccataa ctactacggc attctggtga cgaactggtc
     1501 gtgctataat aacatcaccg acaacctcct aatcgacaat agcagaggtg agatcatcga
     1561 tggatttggc atcggcctat ccctgcagga gaacagctgc cataatatgg tcataggaaa
     1621 tgttgcccgg ggcaactaca acggcctgga gatctccaaa ggctgccagt tcaatgccgt
     1681 ctatgccaat aatgccgctg agaacaagca cggcataagg atgaatgaga accggaataa
     1741 cctcatattt ggaaataatt ttcagtttaa taacatcaat gcctatgaga accagagctg
     1801 caatatctgg aataccactc tgggcaacta ttatagcgat tataagggtg aagatgagaa
     1861 caaggacggc ataggagatg tgccctactc ccttcccgga ccggaatcca gctcctccga
     1921 ccgcatgcct tcgatccgtc ccttcgatga aggaaggctg gagcgggcag atatagggga
     1981 actggtaaaa aaatatgccc tctttgagcc aaaggaggag gcagggactg cagcccggct
     2041 ggtagaaggg gttatggtca tatcccatcc cctgcccagc tctccgcccc agtggaagga
     2101 ctctgagcct ctggatgtga gcagttctcc cttccaggaa gtgaatgatt tttagatgac
     2161 tctccatacg gaaggctcaa tcacattcga agccacgggg gcctttttca atccccggat
     2221 gcaactcaac cgggatatag gggtggccat gaccaaagcc ctgggcatca gcgagtacct
     2281 tgatgcccta tccgccagcg gtattcgggg gatgagggtg gcagcagagg cagggacgga
     2341 gaaagtgact ctcaatgatt tcgatccccg ggcagttcag gtgatgcgaa ggaatgtggc
     2401 ccgaaatcag ctggactgca ttgtcagcga aaaaaacgcc aatgtcctga tgcaccagga
     2461 gcactttcag gcggtagacc tggatccatt cggctctccc tccccctata tctctgctgc
     2521 cagcagatct gcccgctcat acctcttcat caccgctaca gataccgcac ctctctgcgg
     2581 agctcatctg aaaagcggca tccgaaaata tatggctgtt cccctgtgca ccgactatca
     2641 ccgggagatg ggggcccgaa tccttcttgg actggcggcg cgcgagctgg cccggctgga
     2701 caagggtaca gagccgttgc tcacccacgt caccgatcac tatgtgcgca cctaccttcg
     2761 agtggtcaaa ggggccgaag cagcagacag atcattgcag tcactgggat atttggagca
     2821 ctgcaatagc tgcggaagct ttgagactct caggttgctg catcctgtgg gtatatgccg
     2881 ccactgccgt tccagaaccg ccctggccgg gcccttatgg ttgggcaaaa tacaggatcc
     2941 agatgtcatt gccaacgccc tggaagcggg ggatcttggc cccagagcta aaaagatcct
     3001 cagcacctgc gcgaaggagg tgaatgtgcc catgtactat gaccatcata gcatctgtga
     3061 gaagctcaga atcacacccg gcaagattga ggatgtcata tcgaggttga tgtccctggg
     3121 tcatcaggcc tcacgtactc atttcaccgg ccttggcata aagaccaatg ccacccttcc
     3181 cgagctggag gaggttttga gagagaacaa tatttgaaaa tttataaaaa ttgatattct
     3241 ttttttccac ctaaaactta ataaaggaga agagatgtaa tagtgatagc tgcattatga
     3301 taggataatg caggaattcg cggcacgagg tgacgtttat tacccccttc attgctactc
     3361 cctttcgcat ctcgtgccat cccttattac gcattataac atgagtaaaa taatccggga
     3421 ttgctcaggt tttatcctta tatgatccgt tctcgacccg atcctatctt atccattctc
     3481 atcccccaat ggctctgacc caaaaatgta ataatgggct ggataacgtg tcactcatga
     3541 agattgctct tgttggtggg acaggtgaca taggtatggg atttgccctg cgcttgatgt
     3601 gcgctcatga gatcatcatc ggttcgcgca aagaggataa ggccagggag agtgccagtg
     3661 caatcatgca gcttgagggc gcgaatggga atacatgggg aactgataat gccagcgcag
     3721 tagcggtggc ggacgcggtc attctctgtg tcccacctga acatctgaaa tctgtaacct
     3781 atgatctatc cacatccttc aaccatcagc ttgtgatctc tcccgttgtc cccatgagct
     3841 acgatggcaa gttcttccgc ttcaatcctc cgccagaggg cagctccgcc ctgcaggcca
     3901 agtcgctcct ccctccccag ataagagttg tcgccgcttt tcacaccgtg cctgccgcct
     3961 ctctccaggc aagtgataga attctaaaag gcgatgtcct gatatgcggt gacgatgcag
     4021 aggccgtgga tatggtcaag agcttgaccc tggagataaa gaacctccgg cctctatttg
     4081 tcggccctct ggctgcctcg tctcaggtgg agagcttgac ccccatgctc cttaacgtcg
     4141 cccggaagaa taagatcaaa gaggcagggg taagtataat atcggagaga ccgcctgctc
     4201 ctaattgata ttttagatca ttccgctcag agcctctatc accatgcttg gcagctcggt
     4261 cggattgatc aaatccatca ccttccagat gacatagaca ccgatgaagg tgcctgccat
     4321 ggctccgaga ttggacagcg atgccaccaa caggactttg aagaggcggt tattcatcat
     4381 ctggcggtag ctttccgcct ggggaagctc ttttaagtca gagacggtgg gcttgatcct
     4441 ccaggcctct accatgcccg caaaccagcc tgcggccata aatggattga gtgtggtcat
     4501 ccaggcgatg ccaaatgccg ttaaaacaga gagcggatgg ccccgggcta agatcacccc
     4561 cagggctgat agagtgcccg ttatcacaaa ccatatggca aaggctaaga ccaggcttct
     4621 gctggattga gcggtgatca ccaacagggc aatcatcacc aggatggtca atgtcaccac
     4681 cgcaccgaat acccgggaaa aggtgatctt ctttttggcc tttacattca ggctctctat
     4741 ggcgggaatg gcctgggggt ttctcagatg cctttctatt ccctccttat ggccagcccc
     4801 caccactgcc agcaccctgc cttgcctgga gaggctgagc aggtttcggg ccaggtatgc
     4861 atccctctcg tcgaccagaa cgctggccgc cccaggagag atcttgcgaa actcgcctat
     4921 catctgggat acgacatcat cctgagtgat gttatcgata tcgatctcct cttcctcccc
     4981 ccataaggcc ccctggatca gagatccgat cagcctaatc ttgtcgaaga atcccatagc
     5041 cgaccagaag cgttgaatgg taattcccac atccctgtcc accagagcca ccctggctcc
     5101 agtgctctgc gctgcccgaa tcgccgccag catctccgag ccgggcttga cccccatctc
     5161 ctggccgatc ttctgctgca tataggcaag gagcaactgc accaggaaca cgtagagctt
     5221 tccgccactc aacagcttgc cgatctcgat ctcggacttc tcctcctgtc cggtcagggc
     5281 tttgtagcga gagagacaca gctccactgc cactatgtcc ggccttgtct cctcgatcct
     5341 tctgaccact tccagagcgc ttttctctga gacatgcgcc gttcccacta tggttatctc
     5401 gtgggagaac ccctcagtca ggtcggcgcc agcatcaggc tctattatcc tgcatgtcgg
     5461 ctccgggcag tcttcctttg cttccttctt atcaaaatcc aaatcgatta cctcttcagt
     5521 cctgccccga gcatagctcg atggccctga ccaggtcttc tctggagacg atgcccacca
     5581 ggcggccatc agacattact ggcagccggc ggatattcat cttgttcatc atcatcatcg
     5641 cagcagatgc ctcctctgaa ggaccgatca catataactt tcttgtcata acctcagcca
     5701 ctcgggtatc tgcgtgatcc acctggggaa ccctctgcag gtccgaaagg gtcaccacac
     5761 cgatcacttc atcaccggac acgacaggat agccccggtg cttctcctcg aacatatgct
     5821 ctttcaagtc ctgcagggtc atatccggat ccacggtgtg cacatcatct gacattatat
     5881 ccatgaccct cgtgccctcc aggctcacgc tgatctgggt tgccttctcc tcctctgagg
     5941 ctccgacata gacgaagaag gccacaaaga ggaggatgaa gttgaaggag aataggccga
     6001 ggacgaacat gatgatcgcg aagagctttc cgatgctggc cgcccgctgg gtggctttta
     6061 tgtatggcat ctgggtggca aaccaggccc gcaggagacg gcccccatcc atgggaaatg
     6121 cgggcagcag attgaagagc atgaggatga tgttcaagag gcccagcatc cagagcatta
     6181 tgccaagagg gctgccttca tccagcaatg tggcgagaag aattcccatc agacccagga
     6241 tgccgctcac agcaggcccg gcgaaggcca tttggagctc caggcgggga tttctaggta
     6301 tctcctccat ggcagagacc cctccaaaga agtagagggt gatgctcctt atagcgattc
     6361 cattcttgag ggcgatatag ctgtgcccca gctcgtgaag ggccacacag gtgaagagga
     6421 ccaatgagaa gatcagagag taggcccatc tcaggccggc tggctcgatc tcgccaaagc
     6481 catagctcct ccccaggacc tgctggctga aggaggcaaa ggcccaggcg atgtagagga
     6541 tgaccagcag aaaggaccag tgcagctgca ccggtattcc catgatcttc cccaactgca
     6601 atgaagagtt catcatgtgc tagtatcacc caaacattaa taggtctttt gataatttat
     6661 ggcgcaaatg gtgggcacca ggcagaaagc aatttttatt aatttattaa gtgagcgggt
     6721 aggagagtgg gggttcagtg tggattctaa aaactaggat tacccgctcg atttaaaagt
     6781 gtgttggtta agatataaat ttttcccctg ggggtagcaa ttcaccagat atctcaccag
     6841 agagctatgc tctaccatgc aggctgcggt ataggcctcc atcagggtcc cgcctgcagc
     6901 aaatacgcca tgccccctgg cgatgcaggc catatgggac ttaagggcta aagatgctgc
     6961 cttagcgagc atatcggtcc ccagcttgcc ctcgattata ggcacctctc ccagaaagag
     7021 aacgccctcg ctatcgattg gctccacctt ctccatttcc accacagaca ggacaaccgc
     7081 atatggagaa tgggtatgga tgatggccat cttcccagta ttcaggtaaa tcgcccggtg
     7141 aacgcaggtc tctatgctgg caatcttatc catctcacag ggattctcta agctgacctc
     7201 caccaccgtg gacgaatcca gctcgtcgag catcgacccc gtagcggtga tgaatatcct
     7261 gccatcctgg cagatgctga tatttccgaa tcgagagctt gtgagtcctg aggagacgac
     7321 ctttctgcca aaccgcgcga tctcctgcca ggcatcgctc gtcatctcat cacctctcca
     7381 gcagacggaa agggacggct attgactcca tctccccaaa tccacccacc agatcgacgg
     7441 atatcttata atccccggcc gtccagaagc tcgtctccca gcgggcggag tatatgccgt
     7501 tgccatccag gtcgtccatg ggaatctcca gatttctgtc cggcccgatg atcttggtgt
     7561 ttaaggactt gatctctccg gaggcttggg cctctaccat caccggctcg ccttctgtgg
     7621 cctcatcccg gttcagcctt acatcgacca gatccggagg ataaagctcc tctttcgggg
     7681 cgttgatgct cttgagggct cgggtcaggt tgagagctcc atagccatag aggttatcct
     7741 ttccctcctg ccccagctca tctgcggtct tgagcagcag cctcttgata tctgctggtt
     7801 gcagttcagg attcgcctcc agcaaaacag ctgatgctcc ggagacttga ggaacagcca
     7861 ttgatgtgcc gctcactgaa ctcaggccat tcttggagcc agccagggcg gaagggacat
     7921 caacccccag ggtgaccagg tccggcttga tctctccggt cagggtcggg ccgcgtgatg
     7981 attgttcgaa tatcaatccg gtgcgatcaa tggccccaat ggttatcacc ttctctgcgc
     8041 caccgggcat gacaatggag gagggcgaag gcccggagtt tccagcggcg acacacatgg
     8101 tcagcccctc ctcgaccatc cggtcgcagg cctcgtctag cagagatgct ccatcagaag
     8161 gcccttctcc gcccacgcta aacgatatga tctttatccc gtagcgatcc ttattatcca
     8221 ggcaccagtc cagagcttta agagcatcgg atagatagca tgctccatcc ctgtccataa
     8281 ccttgatcac cacaagttcc gctcccggcg ctactcccat gcccaggtcg ccggcgatca
     8341 ggctggcgca gtgtgtgcca tggccgttgt cgtcataagg ttctgtctga ttgttcacga
     8401 agtcctggaa tgcgacgatc ttccgggaca aagcctcatg atcggcatct gcgccggtat
     8461 cgatcagagc cacagtaacc ccctcgccct ttgggccgga gatctctctg ggatcgtcta
     8521 tgatcgaagg cccaggattc gagctgctcg tgctggcagc agagagcacg gtgagcttct
     8581 gatcggtgta cacccctcgg acgccgtcgg tcttggcgat cttttccagg ttgtccgagg
     8641 agacggtcat tgcgctcccg ggcaggactt tgaacctctt ctccagagag gagccgacgc
     8701 cggtaaggtt ccctttaagg tcgtcctcat gaagcacaat gaccttcagg tctttggctt
     8761 tctgctcctc caggctgtct tctattccat tgccattgag atccctggga tcgcggatgg
     8821 tgatcttctt ggactcgtcc ttcgccgcat tgccgaactt gtctacggct atgattgtca
     8881 cattgtaggt tccagcaggc atattggagc cgcaatagcc ggtgaaagtg ccatctcggt
     8941 ctaaatctat catgaggttc atccgattgc ctatatcagc atagaccatt cccaggccgg
     9001 aaggatcgtg gacccgggca tttatctcgc tcctggggtc gcctgtcttg agaatggagg
     9061 ggctcacgct cactccatag atgtccggag cagcgccatc gtcgacgctt ggtcttaggc
     9121 gattttcgta ctccttctga aaccaggcgg ttgccttatt gttctccagg gcattttgta
     9181 tcggaaaagc ggatgcatag ctgaccaggc tgagcaggag aacaaaatgg aggatatggc
     9241 taggattcaa tggtctcact cttaactcca gctatccttt tcgatattct tatatttgct
     9301 ggtgtcattt cacc
//
LOCUS       DAOSGP010000024         9293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_6323_length_9293_cov_12.473046, whole genome shotgun sequence.
ACCESSION   DAOSGP010000024 DAOSGP010000000
VERSION     DAOSGP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6323_length_9293_cov_12.473046"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1114)
                     /locus_tag="PLV49_01445"
     CDS             complement(<1..>1114)
                     /locus_tag="PLV49_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOG97533.1"
                     /translation="GQESPDPTTGSRAVPIYQTSSYVFKSTEHAANLFALKELGNIYT
                     RLMNPTSDIFERRIAAIEGGSGALAVSSGMSAITLALLAITQVGDEIVSANNLYGGTY
                     QLFHYTFPKLGRKVIFVDSTRPDEFKKAITPRTRAIYAETIGNPKLDVPDFEKIARIA
                     HDESLPFVVDNTVGIGIVRPIEYGADIIVDSATKFINGHGNSIGGVIVDSGKFDWSRG
                     KFPEFTQPDPSYHGLKYWDTFGNFPGLGNVAFVFKTRVQLLRDLGPAPSPINSFLFLQ
                     GVETLALRQERHSENALKVARFLKSHPAVAWVNYPGLEEHPSHALAERYLKGKYGALL
                     GFGIKGGLEAGKRFIESVELLSHLANIGDSKSLVIHP"
     assembly_gap    1115..1214
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1215..1909
                     /locus_tag="PLV49_01450"
     CDS             <1215..1909
                     /locus_tag="PLV49_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOG97534.1"
                     /translation="DLILTVGMLAVAFGMLYLIKRTGANLVEPLWGSVIDLIALVMLT
                     ASLASQFMGGHGIVVAFLAGLILSQRRCRKESILNRIYLPVMTILAPVYFVYLGLRTD
                     FAAEFDATIVLLIFAVACAGKIIGAGLGAIAAGIENRKALAVGFGLNARGAMEIALAS
                     AALDYGLIEGQIFVALVIMALGTTVISSLGLPWLMKTGPVSSRAWSRYLCTSRRAVIT
                     IWRARMDYGNLR"
     gene            1916..2155
                     /locus_tag="PLV49_01455"
     CDS             1916..2155
                     /locus_tag="PLV49_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOG97535.1"
                     /translation="MSDIVPDAELDCIGLYCPLPLSMAKENIANIDIGQVLKIQADDP
                     DAEKDIPGWAKRAGHQILMFERSGQVATFWIKRIR"
     gene            2177..3331
                     /locus_tag="PLV49_01460"
     CDS             2177..3331
                     /locus_tag="PLV49_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOG97536.1"
                     /translation="MQGIYLDNQSAMLPDPRVLDFAKRHLIEVPGNPSALHSVGLAAK
                     SAVEEAREKVAELINAEDARNIIFTGSATEANNLAIRGTAMRNASKGMSLLASRIEHI
                     SVLNSMKDLQKNGFQLQLIPVDSKGIVDLQALERMISKQTIVTSVMYANGEIGTIEPI
                     KEISHIVHEKGMLLHVDATAAAGRIPIDVQNDGIDLLTLSSNDICGPQGAGALYAGPD
                     VKIQPVTVGGGQERGLRPGTENLFAIAGMGEAARLARLEMSDDSIRLKVIRDGLIREI
                     LKIDETHLTGHATNRLPHHASFRFDRIEGESILLTLDAMFDIQVSTGSACSSRTLEPS
                     HVLLALGLKHEQAHGSMVMTLGKSNHPDQIPVVFDAVKKTVARLRALSPL"
     gene            3347..3787
                     /gene="nifU"
                     /locus_tag="PLV49_01465"
     CDS             3347..3787
                     /gene="nifU"
                     /locus_tag="PLV49_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOG97537.1"
                     /translation="MAQIGYSKKVMDHFMNPRNVGVIDDADGYGKVGNPVCGDLMEIF
                     IKVKDERINDIKFRTFGCGSAIAVSSMVTEMAKGKLLDEAMKITRKDVADELDGLPPQ
                     KMHCSNLGADALHAAIQDYWVKTGKIKLDEVKKEDDGEGDACPT"
     gene            complement(3801..4127)
                     /locus_tag="PLV49_01470"
     CDS             complement(3801..4127)
                     /locus_tag="PLV49_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97538.1"
                     /translation="MRKLDARSLEILRKLAPEISEPVHYRSILPPVSMHHAADDEDFQ
                     ERLERLSAEEFDYLAGKIMDGSECLNCISPESARIFIDLLEKKAPGGSGERIRELYKS
                     STGYEV"
     gene            4258..5133
                     /locus_tag="PLV49_01475"
     CDS             4258..5133
                     /locus_tag="PLV49_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97539.1"
                     /translation="MIATDPATSIDFASLKSGGIIMQKEDEFFAIRLKLTGGTISADK
                     LPRIAEVAQRYGRGEVRLTARQGIEIPWIKFEYIEAARKSLAESSLALGPCGARFRAV
                     TACPGLPICKKALADSQRFARMIDSRFSGLDLPDKFKATVSACPNACSRPFESDIGFC
                     AMAEPRLERDDCNGCSLCLDICREEALTLEDGRPVLKGDRCISCADCIDCCPTSAWKA
                     EKSGYLVYAGGRMGRHPMLGKKIADFVDEEQGMKVIHRCLDFYRQYGNRRERFGDLII
                     RAGMDEFLDFVMKEG"
     gene            5135..5965
                     /locus_tag="PLV49_01480"
     CDS             5135..5965
                     /locus_tag="PLV49_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOG97540.1"
                     /translation="MSGSFTDEQIRRYSRQIILPDVGGIGQRKLLGSRVLIIGAGGLG
                     SSAIAYLAAAGVGTLGIIDSDIVEISNLQRQIIHGGNIGLAKSISAESFVRRLNSDVD
                     VVAYQACFSAENARTIIREYDFVIDGSDNFPTHYLINDACVLEKKPCSHGSILRYEGY
                     ATTSIPERGPCYRCIFKKIPPPGLIPSCQEAGVFGVLPGIIGTIQASEALKFVLGTGQ
                     TLTGRLLCFNALDMTFFEASASKRLDCSICGEYPRIVSIEPENYLEEGREECRLDPRA
                     "
     gene            5949..6206
                     /locus_tag="PLV49_01485"
     CDS             5949..6206
                     /locus_tag="PLV49_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOG97541.1"
                     /translation="MTLELDLRGEVCPYTFVKTKLKMEEMAGGEELIILLDDTEATAN
                     VSRSLKSEGHDIVDLKSISRGVWQITAKKAGFQTQSKERDE"
     gene            6247..7269
                     /locus_tag="PLV49_01490"
     CDS             6247..7269
                     /locus_tag="PLV49_01490"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOG97542.1"
                     /translation="MMKAIILLSGGLDSTLAAELMSREGLELIAVNFKTPFCLCDRHS
                     SGLGCGSNARRVADSLGIELKIINATEDFLKVLEKPAHGYGANMNPCIDCRILFFKKS
                     RELMEQSGSSFIITGEVLGQRPMSQFRRQMNLIEREAGLEGLVVRPLSAKLLAPTIPE
                     KNGWISRERMLDIAGRSRRPQMALARDLGINDYPCAAGGCLLTDPKFAGRIKDLIDHG
                     ELDMQNISLLKSGRYFRLSQNAKLIVGRNEGENKMLAILAKDGDILFEPLGINGPVAV
                     GKGPFSSQLLDVSCRIMARYCDRNGKNYADISYRRLPDDQVMTVRSVCLDEGDLAKLR
                     ALTNAA"
     gene            7389..8288
                     /gene="pdxS"
                     /locus_tag="PLV49_01495"
     CDS             7389..8288
                     /gene="pdxS"
                     /locus_tag="PLV49_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOG97543.1"
                     /translation="MALELENLRFGTELLKRGFAKMQKGGVIMDVTTSEQAVVAEQAG
                     AVAVMALHAVPADIRKMGGVARMADPAIIEQIINAVTIPVMAKARIGHFAEAQILEAL
                     GVDMVDESEVLTPADQFHIEKTRFTIPFVCGARNLGEALRRIKEGAAMIRTKGEAGTG
                     DVSQAVLHMRMIQGQIRSLKGMDDLQLLKVSRDIEADYDLVVECARLQRLPVVNFAAG
                     GVATPADAALMMQLGADGVFVGSGIFKSEEPEAMAKAIVQAVHNFDNPQLLAQVSKGL
                     GAAMKGLDATLIPEGEVLQVRGW"
     gene            8483..>9293
                     /locus_tag="PLV49_01500"
     CDS             8483..>9293
                     /locus_tag="PLV49_01500"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG97544.1"
                     /translation="MARLGLKDITVSYGTNGKRTTALHNINLNIEDGDFVSLIGPSGC
                     GKSTLLDLVAGLRTPSSGQVLIDGHEICGPGPDRGMVFQDYSLFPWMSALENVRFAVE
                     HSWARTDKDESLEIANRYLDVVGLSSFRHKYPNTLSGGMRQRLAIARMFAMNPEIFLM
                     DEPFGALDSLNRVFMQDLLVRLWSKDAKRKTVLFVTHDVDEAILLSDKVVVMTPTPGK
                     IKEVVKVPFSRPRKRQSLFVSQDYIILRSKLLARFSEEMQKDLEKQEKELIT"
BASE COUNT         2244 a         2221 c         2587 g         2141 t
ORIGIN      
        1 gggtgtatca ccaggctctt ggagtctccg atgttggcca gatgggaaag cagctccaca
       61 gactcaatga acctcttgcc tgcctccagg ccgcccttga tgccaaagcc cagcagagct
      121 ccatacttgc ccttcagata tctctcagcc agagcatggc tgggatgctc ctccaggccg
      181 ggataattga cccatgctac ggcagggtgg ctcttcagga accgcgccac cttcagcgca
      241 ttctccgagt gcctctcttg ccgcagagcc agagtctcca cgccttgaag gaagaggaag
      301 gaattgatgg ggcttggggc cggcccgagg tctcggagaa gctgcactct ggtcttgaag
      361 acgaaagcca catttccaag tcccgggaag ttgccgaatg tgtcccagta cttaaggccg
      421 tggtagctgg gatcgggttg tgtgaactct ggaaacttac cacgggacca gtcaaatttg
      481 cctgagtcca caataacacc accgatggag tttccgtggc cattgatgaa cttggtagca
      541 gaatccacga ttatgtctgc gccgtattcg ataggcctga ctatgccgat tcctactgtg
      601 ttgtccacta caaagggaag gctttcatcg tgagctatcc tggcgatctt ctcaaagtcg
      661 ggcacatcca gcttgggatt gccgatagtc tcggcataaa tagcccgggt cctaggtgtg
      721 atggccttct tgaactcatc cgggcgggtg gagtcaacga aaataacctt tcgtccaagc
      781 tttgggaagg tataatggaa gagctgatag gttccgccat aaaggttgtt ggcggagacg
      841 atttcatctc ccacttgggt gatggccagg agcgccaggg tgatggcaga catgcccgat
      901 gacaccgcca gagcaccgct tccgccctct atggctgcga tgcgccgctc gaatatatcg
      961 ctggttggat tcatgagtct ggtatagata ttgcccaatt cctttagagc aaagagattc
     1021 gctgcgtgct ctgtgctctt gaatacgtaa gacgaagtct ggtagatggg aacagctctc
     1081 gatcccgtgg ttgggtccgg tgactcctgg ccgannnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnntggatc tcattctcac tgtcggaatg ctggccgtgg cctttggcat
     1261 gctctacctc atcaagagaa cgggagccaa tctcgttgag ccgctttggg gtagcgtcat
     1321 tgatctcatt gctctggtca tgctgacggc atcgctggct tctcagttca tgggcgggca
     1381 cggcatagtg gtcgcatttc tggctggact gattctctcg cagagacggt gccgaaagga
     1441 gagcatctta aacaggattt acctgccggt tatgaccata ctggcaccgg tatatttcgt
     1501 atacctgggc ctcagaacgg actttgcggc tgagtttgat gcaaccattg tccttctgat
     1561 atttgccgtt gcctgtgcag gcaagatcat cggcgcaggt ttgggtgcca tagccgcagg
     1621 catagagaat cgaaaggcat tggctgtcgg ctttggactg aatgcccgtg gagcaatgga
     1681 gattgcactg gcctcggctg ccctggatta cggattgatc gaagggcaaa ttttcgttgc
     1741 cctggttatc atggcactgg gaaccacggt gatttccagc cttggtcttc cctggcttat
     1801 gaagacgggt ccagtatcct cgagagcctg gagccgctac ctctgcacga gcaggcgggc
     1861 agttataact atttggagag ctaggatgga ttatgggaat ttaaggtgaa tctaaatgtc
     1921 agatattgtg ccagatgctg agctggattg cattggcctg tactgtccgc tccccctctc
     1981 tatggccaaa gagaatatcg ccaatatcga tatcgggcaa gtgcttaaaa tacaggctga
     2041 cgaccccgat gccgaaaagg atattcctgg atgggccaag agagctggcc accagatcct
     2101 gatgttcgag agatcaggcc aagtagcgac cttctggata aagagaatta ggtaataggg
     2161 tgataaagtg agctttatgc agggaatata tttggataat caatcggcta tgctccccga
     2221 tccaagggtg ctggattttg ccaaaaggca tcttattgag gtgcctggaa atccgtcagc
     2281 tctgcattcg gttggtctgg cagccaaatc agccgtcgaa gaagccagag aaaaagtggc
     2341 cgaactgatc aacgctgaag atgctcgaaa tatcatcttc actggaagcg ccacagaagc
     2401 caataacctg gccattcggg gaacggcaat gagaaacgcc tccaagggaa tgagcctctt
     2461 agccagcaga atagagcaca tctccgttct caattctatg aaggacctgc agaaaaatgg
     2521 cttccaactc caactcattc ccgtggactc caaaggcatc gtggatctgc aagctctgga
     2581 gagaatgatc agcaaacaga ccattgttac ctctgtaatg tatgccaatg gcgagatagg
     2641 cacaattgag cccataaagg agatcagcca tatagtccat gaaaagggga tgctcctgca
     2701 tgtggatgct acagctgctg cgggcagaat acctatagat gtgcagaacg atggaataga
     2761 cctcctgacc ctatccagca acgatatatg cggcccgcag ggtgcaggcg ctttatatgc
     2821 aggccccgat gttaagattc agccggtcac tgtgggtggc ggccaggagc gaggactgcg
     2881 accgggcacg gagaacctgt ttgccatagc tgggatgggt gaggctgctc gtctggcaag
     2941 actggagatg agtgatgata gcatccgcct gaaggtcatc cgagacggtc tgatcagaga
     3001 gattcttaaa atcgacgaga ctcatctgac cggccatgcc acaaatcggc ttccccacca
     3061 tgccagcttc aggtttgaca ggatcgaagg agagagcatt ctgctgaccc tggatgccat
     3121 gttcgatatt caggtatcca ccggctctgc ctgctcatca aggactctgg agccaagcca
     3181 tgttcttttg gccttgggcc tcaagcatga gcaggcgcac ggctcgatgg ttatgacttt
     3241 aggaaagtcg aaccatcccg atcagatacc agtggtcttt gatgcagtga agaagactgt
     3301 ggcgaggctg agagccctct ctccactcta atgaggtgaa tgcataatgg cacagatagg
     3361 ttactccaag aaggttatgg atcactttat gaaccccagg aacgttggtg tgatagatga
     3421 tgcggacggc tatggcaagg tcggcaatcc ggtttgcggg gacctcatgg agatattcat
     3481 caaggtcaag gacgaaagga tcaacgatat aaagttcagg acatttggtt gcggatcggc
     3541 cattgctgtc tctagcatgg taacggagat ggccaaaggc aagctgctgg acgaggctat
     3601 gaagatcact cgcaaggacg tggcagacga gctggatggc ctgccgcctc agaagatgca
     3661 ctgctccaat ctgggagctg atgctctgca tgctgccatt caggattact gggtcaagac
     3721 gggaaagata aagctggatg aggttaagaa agaggatgac ggagaaggcg atgcctgccc
     3781 tacctgatta tcagggccca ctaaacctcg tatccagtgg atgatttgta aagctctcta
     3841 atccgctccc cggaccctcc aggagccttc ttctccagga ggtcgataaa tatccgggcg
     3901 gattccggag agatgcagtt cagacactcc gacccgtcca ttatcttgcc tgccaggtaa
     3961 tcgaattctt ccgcggagag cctctccaga cgttcctgga aatcctcgtc atcagctgcg
     4021 tgatgcattg acacaggggg aaggatggat cggtagtgaa caggctcgct gatctctggc
     4081 gcgagctttc tcaatatctc caggctcctg gcatctagtt tcctcatgcg tcttccaagt
     4141 atgcaagagg ataattaaat ctttcttgcc gggcttccct ctccatatgc gcgagatatt
     4201 gccgcaatgc aaaccttcat tcaactcttc caaccgtcac tccaaagagg gcacaaaatg
     4261 attgctacag atcctgcaac aagcattgac ttcgcctcac tgaaatccgg cggtattatc
     4321 atgcaaaagg aagacgagtt cttcgccatc cggctcaagt tgacaggcgg cactatctct
     4381 gcggataagc ttcccaggat tgcagaggtc gcccaaagat acggccgggg cgaggttcgt
     4441 ctcactgccc ggcagggaat agaaatcccc tggatcaagt ttgaatatat cgaagccgcc
     4501 aggaagagtc tggcagagtc gagcctggct ttgggccctt gtggcgctcg ctttcgagcg
     4561 gtgaccgcct gtccgggtct ccccatctgc aaaaaggctc tcgcagacag tcagaggttt
     4621 gccaggatga ttgatagcag attctccggt ctcgatctgc ccgataagtt caaagccaca
     4681 gtctctgcct gtcctaatgc ctgctcccgg ccttttgaga gcgatatcgg cttttgcgct
     4741 atggcagagc ctcgactgga gagagatgac tgcaatggct gcagcctatg cctggatatc
     4801 tgtagggaag aagcccttac tctggaagac ggccggccag tgctcaaagg cgataggtgc
     4861 atctcttgcg cagactgcat cgattgctgt cccaccagtg cttggaaggc ggaaaaatca
     4921 ggatacttgg tctatgcggg aggacggatg ggcagacacc caatgcttgg aaaaaagata
     4981 gccgacttcg tagacgagga acagggcatg aaggtaattc atcgttgcct ggatttctac
     5041 cggcaatacg gcaacagaag agagcgattc ggggatttga tcattcgtgc aggcatggat
     5101 gaattcctgg attttgtcat gaaggaggga taaggtgtct ggatcattca ccgatgagca
     5161 gataaggcgc tactcgcgcc agattattct tccagatgtg gggggcattg gacaaagaaa
     5221 gcttttgggt tctagagtgc tgataattgg cgcagggggt ctcggctcct cagccatcgc
     5281 ctatctggct gccgcaggag tgggaacgct tgggattatc gacagcgata tagtggaaat
     5341 aagcaacctg caaaggcaga tcattcacgg cggcaatatc ggcctggcca aatcaatatc
     5401 cgcagaaagc ttcgtgcgca gactgaatag tgacgtggat gtggttgcct atcaggcgtg
     5461 cttttctgcc gaaaacgcca ggacgatcat ccgggagtat gatttcgtga tcgatggaag
     5521 cgacaatttt cccactcatt atctgatcaa cgatgcatgc gtgctggaaa agaagccttg
     5581 ttctcacggc agcatcctca gatatgaagg atacgctacc acctccattc ccgaaagggg
     5641 gccatgctat cgctgcatct ttaaaaaaat cccgcctccg ggattgattc cgagctgcca
     5701 ggaagctggc gtctttggag tgctgccggg catcatcggc actattcagg cctcagaagc
     5761 cctgaaattc gttctgggaa ccggccagac tttaaccggt cggctgctat gcttcaatgc
     5821 ccttgatatg accttttttg aggcttcagc cagcaaaagg ctagactgca gcatttgcgg
     5881 ggagtatcct cggatcgtca gcatcgagcc agagaactat ctggaggaag gacgggagga
     5941 gtgcaggctt gaccctagag cttgacttga gaggagaggt ctgcccttac acctttgtca
     6001 agacaaagct gaagatggag gaaatggcag gcggtgagga gctgatcatc ctgctggatg
     6061 acacagaagc aacggccaat gtctcaagga gcctgaagag cgagggtcat gatattgtcg
     6121 atctgaaatc catatcgcgc ggagtctggc agattaccgc aaagaaggct ggatttcaga
     6181 cccagagcaa ggagagggat gaatgagaac tatctgctcg atcgtatcac tgcagaggtc
     6241 tgatttatga tgaaagctat aattcttctt tcaggcgggc ttgacagcac tctggccgca
     6301 gagctcatga gccgagaagg gctggagctt atagctgtaa acttcaagac ccctttttgc
     6361 ctttgcgaca ggcattcctc cggtctgggt tgcggcagca atgcccgccg ggtggctgat
     6421 tcactgggaa ttgagctaaa gatcattaac gcaactgagg atttcctgaa agtgctggaa
     6481 aagcctgcgc acggctacgg tgcaaacatg aatccatgca tcgattgcag gatactgttc
     6541 ttcaagaaga gccgcgagct gatggagcag tctggttcat ccttcatcat cacaggagag
     6601 gtgctgggcc aaaggcctat gagccagttc aggcgccaga tgaatttgat cgagagagaa
     6661 gccgggctgg agggtttggt ggtccggccc ctttcggcaa agctccttgc ccctactatc
     6721 cctgagaaga acggatggat aagccgggag aggatgttgg atatagctgg gcgctccagg
     6781 aggccgcaga tggctttggc cagggatctt gggatcaacg actacccctg cgctgccgga
     6841 ggatgccttc tcaccgatcc caagttcgct ggccggataa aagacctcat cgatcatggt
     6901 gagctggaca tgcagaacat cagtctattg aaatcaggca gatacttccg tctctctcaa
     6961 aatgcgaagc ttattgtggg ccgaaatgaa ggagagaata aaatgctggc tatactggca
     7021 aaggatggcg acatactctt tgagccgctg ggcatcaacg gtccggttgc cgttggaaag
     7081 ggcccttttt cctcacagct gctggatgtc tcctgtcgta tcatggcccg gtactgcgac
     7141 aggaatggca agaattatgc agacatttct tacaggaggc ttcctgacga ccaggtgatg
     7201 acggtgaggt cggtatgcct ggacgaagga gatttggcca agctgagagc attaacaaat
     7261 gcagcttgat agcattgtgg acaattattg ccgcaaagac caatttgcct taatgcacag
     7321 ctattaatct ccatcaacaa tttcgcaccc ctaatttaga agcagcgaaa gaagctagag
     7381 gtaattttat ggcactggaa cttgagaatt tgagatttgg aacggagctg ctcaagcgcg
     7441 gctttgccaa aatgcaaaag ggcggtgtga tcatggatgt caccacatcg gagcaggcgg
     7501 ttgtagctga gcaggctgga gcagtggcgg tcatggcact ccatgccgtt ccagcggata
     7561 tcagaaagat gggaggtgtg gccaggatgg cagatccagc cataatagag cagatcatta
     7621 atgctgtaac catacctgta atggccaaag ccagaatcgg gcacttcgca gaggcccaga
     7681 tcttggaggc tctcggtgtt gatatggtgg acgagtccga ggttctcacg cctgctgatc
     7741 aattccacat cgagaagacc aggttcacta ttccctttgt ctgcggtgct cgaaatttgg
     7801 gcgaagctct gaggcgaatt aaggagggtg cggctatgat caggaccaaa ggcgaggccg
     7861 gtaccggcga tgtgagccag gcggttttgc atatgagaat gatccaaggg cagataagga
     7921 gcctgaaggg catggatgat ctgcagcttc taaaggtttc ccgcgatatc gaggccgatt
     7981 acgaccttgt tgtggagtgt gctcgactgc agcgcttgcc tgtggtcaac tttgccgctg
     8041 gaggcgtggc aacccctgcg gatgctgctc tgatgatgca gcttggagct gatggtgtat
     8101 tcgtgggttc agggatcttc aagtctgagg agccagaggc aatggcaaag gcaatcgtcc
     8161 aggcagtgca taattttgat aatccgcaac tcctggccca ggtttctaaa ggtcttggtg
     8221 cagccatgaa gggtcttgat gcgacattaa ttccagaggg agaagttctg caggttcggg
     8281 gctggtagtt cctgtacaag aaagcgggct aattgatcca attttcaagg aggcgagggg
     8341 gaggatgtac agcagacagt ctttttaaaa cacgatgagt tctcatgcgt catctattaa
     8401 gccctatttt tgcggcagct tttgctcgat tctcaaagca tttacgtcac aaatccctaa
     8461 tcacaggcaa aggagctcac ttatggcaag attagggctg aaggatatca ccgtctccta
     8521 tgggaccaac ggaaaaagaa caactgcgct gcataacatc aacctcaaca ttgaggatgg
     8581 cgattttgtc tcgctgatcg gcccaagcgg ttgtggaaaa agcactctgc ttgacctcgt
     8641 ggcaggtcta cgcaccccct ccagcggcca ggtactcatc gacggccacg agatctgcgg
     8701 tcctgggcct gatcgaggca tggtgttcca ggattattct ctctttccct ggatgagcgc
     8761 tctggaaaac gtgcgttttg ctgtagagca ttcatgggcg agaaccgata aggatgagtc
     8821 gcttgaaata gccaatcgat atcttgatgt cgtagggctg tccagcttcc gccacaaata
     8881 cccaaatact ctctctggag gaatgcgcca gcgcctggcc attgccagga tgttcgccat
     8941 gaatccggag atctttctga tggacgagcc ttttggagct ttggattcgc tcaatcgggt
     9001 attcatgcag gacctcctgg ttcgtttgtg gtctaaagat gcgaaaagaa agaccgtgct
     9061 ctttgtcact cacgatgtag atgaagctat cttgctctcg gataaagttg tggtcatgac
     9121 gcccactccc ggaaagataa aagaggtcgt gaaagtgccc ttttccaggc ctagaaagag
     9181 gcagagcctt ttcgtgagcc aggattacat aatcctccgc tccaagctcc tggcccgttt
     9241 cagcgaagag atgcaaaagg acctggaaaa acaggagaag gagctaatca cct
//
LOCUS       DAOSGP010000025         8929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_6666_length_8929_cov_14.500451, whole genome shotgun sequence.
ACCESSION   DAOSGP010000025 DAOSGP010000000
VERSION     DAOSGP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6666_length_8929_cov_14.500451"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLV49_01505"
     CDS             complement(<1..439)
                     /locus_tag="PLV49_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97545.1"
                     /translation="MTLYTALIATVEISQVMIPPIVIGFFLANVIRRSPYFRYLTMPT
                     AWLAAVSRLPAECSVALTLFLINSWAALALLSEVHRKRQITDCELIVAVLVGFIPKGL
                     NSMLIFSLPVALSVLGPQIGGLYVCLDLLANFFVAGIGILAARM"
     gene            complement(497..2248)
                     /gene="cydC"
                     /locus_tag="PLV49_01510"
     CDS             complement(497..2248)
                     /gene="cydC"
                     /locus_tag="PLV49_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HOG97546.1"
                     /translation="MIRDVLDSRSVWRRLIYPIRPHLGLMSLAVISEILRQVSGIGVA
                     VLGAALFARAVAGTATGELYPYAAAMIILGLARGTFGYLGPYLSHVAAFRILVDLRDE
                     FFWAIEPLAPAKLARRRTGDLVSTAVSDIELLELFFAHTAGPAVVAFIVPIIALTALA
                     TINLLLAEALLIFLILLILMPRLAFWLGTTLGERLRGQQALLNSQELDTIQGMKEILS
                     FGYSRQRIEELSENSATLLALQARQARNIGLQSAANISIVSAGTLAVLLCASILVRQN
                     SLAPGFLPITMILASSVFLSITSVVEISKQLSLTRAAARRLFLLLDEQPAVRDESGLG
                     PAVPIESIEPSISLQDVSFRYAPDEPLILSSISLEIPAGSTVALVGTSGAGKSTVINL
                     MLRFWDPEAGRILLGGSDLRCYPMMQLRRLFSVVSQDVFLFSDSIRENIRLGRAEASD
                     AEVEEVAIRARIHDFITTLPQGYDTLVGERGVRLSGGERQRIAIARALLKGAPILVLD
                     EATSSLDAENERAIKEALMERREGRTTIMIAHRLSTVVDADWIFVINEGRVVEQGRHE
                     DLIARKGRYARLVEAQS"
     gene            complement(2245..4056)
                     /locus_tag="PLV49_01515"
     CDS             complement(2245..4056)
                     /locus_tag="PLV49_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG97547.1"
                     /translation="MEGLIDKRILKFAPGARRWIALTFLSGLMGSIANIALLFLAGRI
                     IVGIYDGRTLLSMLDLFIGMLAAISLRAIAEASRDITSQRSAAMVKSSVRKRINQHLL
                     DLGPSFVEMRNTSALATTVLDGVEALEAYVGLYIPYMALCLVMPILLFLGFAIYVDLI
                     SALMLMAFVPLVPLSLLIFTNLEEWKVGRRAWETYRELSAYFAESLQGLATLKLFSQA
                     ERRGQELHRRSRNLERALVQSLQLYFGASFVSEVVPVLGYSLTMIVSSFRLSQGSLSM
                     DKLVMVLLLGSLFYEHVSHLLLYHHYSLLGKRTANAIFELLDQMPEVRDGQDTAPSAI
                     EPEIRFEGVHFSYHGDRPVLHNISFEVKPGSTVALVGATGTGKSTVIDLLFRFHDPQE
                     GRVLLGGHDIRSLPLNFLRSQMALVAQESYLFYDTVENNLRLGNPKASHEEIVRAARA
                     ANAHQFIVALPEGYQTVIGERGVLLSGGERQRIAIARALLKGAPILLLDEPTSSIDAE
                     NEEDIQKALGRLQAQRTVMIIAHRLSTVRKADCILVMEMGRIAESGSHEELLAKGGIY
                     AHLVAAQSLDMPECRDDENALPMKVGSNLAAVAGGQP"
     assembly_gap    4170..4269
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4476..5762
                     /locus_tag="PLV49_01520"
     CDS             4476..5762
                     /locus_tag="PLV49_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG97548.1"
                     /translation="MIPLKALMLAFALLMLSASGNGYVLHIFGNANLDDDINDQDLAY
                     LERIIEGTEEPTWLSDADNNGEIDESDISLVESIINGTPSEITIVDSDNKTVTVKQPL
                     ERLVVYTHQCAEILQLLGAEEKVVGVRDTFAEQPNRFPRLSKYQSIGSGGEPDVEAVL
                     KTNPDAILAYTWYPSEESLDDKLPPEVEVLRFDCECSGQGPDDMRERVRLLGILMGAK
                     DRAEEYLQWHDAILDQVEDEVRKIPQNERVRVYLESTPEGDRPMSSRTAIGTGHAAHK
                     LVEMAGGVNIAAGRLPGYEDTAFEYGEIETEWVLSQNPEVIVGRAMGPGIRPYENVND
                     SLLESYAQDIRSLPGFDEVRAVKNDGIYIITNDYAITPNYPSAVLLLARWFYPDRFAD
                     IDPQAAHQEYVNMMGLDYDVLEKGAFFFSEEQAEDL"
     gene            5821..7197
                     /locus_tag="PLV49_01525"
     CDS             5821..7197
                     /locus_tag="PLV49_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG97549.1"
                     /translation="MREDKIFVLALVLLANIIPLSSGSGYVLHVFGNANMDGTIDWED
                     IATLREIISNNISPTDLADANLDGEVDLRDIAQVEMIINGTERNLSLLDGNDLPITIN
                     KPVERIVVEYLDNADLMQILKKTDKVVGVDLAVAKSPAEFPEMSNRTCVGAMHKEPDY
                     EKVLSLDPDLLLVFSNVTQEKNKNLPGVPVLFAGLYYPDLLKPETSAFTDAVRKLGYV
                     LDAQQDAEEYIQWHMNSLNRLVETTGSIPDSERPTVLVAAYPEADEKTIFTFAQIDTL
                     TSMVDLAGGRTVAADLPDFMKSTYRIEVDPEWVLQEDPDYIVLLVVATTYSGLVLDPP
                     SGYDADDPTGMKEALEAFMSRPEYANLKAVQNGDVYILSGNLRNDASKGLIGAAYLAR
                     IFHPDEAEMDPEDLHQEYLQRFLGLDYDLDEHGVFIYPPLIKGPGELEGVPDGYYDAV
                     SYEEAAKA"
     gene            7399..8754
                     /locus_tag="PLV49_01530"
     CDS             7399..8754
                     /locus_tag="PLV49_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG97550.1"
                     /translation="MKGCLIAVMMLCGLLAQASAGDYILHIYGNANMDGTIDEKDIEY
                     LQEIIAGSCNASPLADANFDGKVDENDISQVKMIIDDSQENITILDGNGMPVTVRLPV
                     ERAIVEHLDNSEMMMILKRTDKVVGVDLAMSKSEKEFPDLVKKTGVSGFSPATDPDYE
                     QALSLSPDLLLTFSNKTAEKEEKMPEVPVVFAGLYYPNLINPENSSFTDAVHKLGYIM
                     QAREQAEEYIDWHISKIDEIKSYTANLAEEKRPRVLIATLPKEGDNSIFTYAKIDTLS
                     QMVTLAGGRSIAEDLPAYLESSYRIEVDPEWVIKSDPDYMIFITIVLTPPIGYDSDDI
                     SGISQALLSFKSRPEYANLTAVKNNQVYIINGNLRNDASKGLIGAAYLARIFHSDNVD
                     LDPEDLHQEYLQRFLGLDYDLDEHGVFIYPPLIKGPGELEGVPDNYYQSYANDSEARA
                     Q"
BASE COUNT         2198 a         2252 c         2424 g         1955 t
ORIGIN      
        1 tcattctggc ggcaagaatc ccaatgcctg ccacgaagaa gttggccagt agatccaggc
       61 atacgtaaag gccgccgatt tgggggccca gaacggagag ggccacaggc agcgagaaga
      121 tgagcatgct attcaatccc ttgggaataa agcccactaa aacggccaca atcagttcac
      181 aatcggtgat ctgcctcttt ctgtgcacct cggatagaag ggcaagggca gcccagctat
      241 tgatcagaaa gagggtcagg gcgacagagc attccgctgg aagacgggat acagccgcca
      301 gccaggcagt aggcatggtc agatatcgaa aatagggtga gcggcgaatg acattggcca
      361 ggaaaaatcc aatcacgatc ggtgggatca ttacctggga tatctccaca gtggcgatga
      421 gagcggtata aagggtcata tcgatagaat atattgatat atttatatta ttatttggcg
      481 atcgcatgtg cctcaactaa gattgcgcct ccaccagccg ggcatacctc cctttccggg
      541 cgatgagatc ctcatgcctt ccctgctcta ctacccgtcc ctcatttatg acgaagatcc
      601 agtctgcatc caccacagtg ctcagccggt gagcgatcat gatggtggtg cgaccctctc
      661 tcctttccat cagagcctct ttgattgccc tctcgttctc tgcatccagg ctggaggtgg
      721 cctcatccag gacgagtatg ggcgctcctt tcaggagagc tctggcgatg gcgatcctct
      781 gtcgctctcc accgctgagg cgaactcccc tctctcccac cagggtatca tacccctggg
      841 gtagggtggt aataaaatca tgaatgcggg ccctgatagc gacctcttcc acctcggcgt
      901 cgctcgcttc tgcccggcct aaccggatgt tctctctgat gctatcgctg aagaggaaga
      961 catcctgaga aaccacagaa aacaaacggc gtagctgcat catgggatag cagcgcagat
     1021 cacttccacc gagcaatatc cttcccgctt ccggatccca aaaacggagc atcagattga
     1081 tcactgtgct ctttcctgct ccgctcgtcc ccaccagtgc cactgtcgag cccgctggaa
     1141 tctccaggct gatggagctt aagatcaaag gctcatccgg agcatagcgg aagctgacat
     1201 cctgcagaga aatggatggc tctatggatt cgatcggaac tgccggaccc agaccggact
     1261 cgtcccttac cgctggctgc tcatccaata gaagaaagag tctgcgagca gcagcgcggg
     1321 tcaaagagag ctgcttggag atctcgacca ctgaggttat ggagagaaag acgcttgagg
     1381 ccaggatcat ggtgatggga agaaagcctg gcgcaaggga attctgccgg accaagatgc
     1441 tggcacatag gagcacggca agagtccctg cagagactat gctgatattc gctgcgcttt
     1501 gcagcccgat gtttctcgcc tgcctggcct gcaatgccag cagtgtggca gagttttcag
     1561 atagctcctc tattctttgt ctgctgtagc cgaaagacag gatctccttc attccctgaa
     1621 tagtatccag ctcctgggag ttgagaagtg cctgctgccc cctcaacctc tcccctagag
     1681 ttgtgcccag ccagaaagcc agcctgggca tgaggatcag caggatgaga aaaatgagca
     1741 atgcctctgc caaaaggagg ttgattgttg ccagagcggt gagagcaatg atggggacta
     1801 taaaagccac taccgcaggt cctgctgtat gggcgaagaa gagctccagc aattctatgt
     1861 cggatactgc tgtgctgacc agatcgcccg tgcgcctcct ggcaagcttt gccggagcga
     1921 ggggctctat ggcccagaag aactcgtccc tcaggtcgac caggatgcgg aatgccgcca
     1981 catgagatag ataagggcct aaatagccaa aggtccctct ggccaggccg aggatgatca
     2041 tggcggcagc atagggataa agctccccgg ttgccgtgcc ggccacagct cgggcgaaga
     2101 gggctgcacc aagaacggca acgccgattc ccgagacctg tcttaagatc tcggatatga
     2161 cggccaggct catcagtccc agatgagggc gaataggata gatcagccgg cgccaaaccg
     2221 atctggaatc gagaacgtct cttatcatgg ctgtcctccc gccacagctg caagattgct
     2281 gccaactttc atggggagag cattctcgtc atccctgcat tctggcatat ccagggactg
     2341 ggccgccacc agatgggcat agatgcctcc tttagccagc agctcctcat ggctgccgga
     2401 ctcggcgatt ctccccattt ccatgactaa aatgcagtcc gcctttctga ctgtgctcag
     2461 ccgatgggcg atgatcataa cagtgcgctg ggcctgcaga cggccgaggg ccttctggat
     2521 gtcctcttcg ttctcggcat cgatgctgga ggtcggctca tccaggagga gaatcggtgc
     2581 ccccttgaga agggcgcggg caatggcgat tctctgccgt tcccctccgg agagaagcac
     2641 tcccctctcg cctatgaccg tctgatagcc ttcaggaagc gctacgatga actgatgagc
     2701 gttggctgct cttgccgccc ggacgatctc ttcatggctg gccttgggat ttcccaagcg
     2761 caggttattc tccacggtat catagaaaag ataggactcc tgagccacca gcgccatttg
     2821 gctgcgcagg aaattgaggg gcaagctgcg tatatcgtgg cctcccagga gcactctgcc
     2881 ctcttgggga tcgtggaagc ggaagagaag atcgatgact gtgctcttgc cagttcctgt
     2941 cgccccaacc aaagccaccg tgctgcctgg cttcacctca aatgagatgt tatgcaaaac
     3001 cggcctatcg ccgtgataag agaagtgaac cccctcaaac ctgatctccg gctcaatagc
     3061 agaaggtgct gtatcttggc catcccggac ctcaggcatc tgatctaata gctcgaatat
     3121 ggcgttcgct gttctttttc ccagcaggct gtagtgatga tataaaagca gatggctgac
     3181 gtgctcgtag aagagcgagc ccagcaaaag gaccatgacc agtttgtcca tcgataggct
     3241 accctggctg aggcggaatg atgagacgat catggtcagg gagtagccca gaactgggac
     3301 cacctcggag acgaagctgg ctccaaagta gagctgcagg ctctgcacca atgccctctc
     3361 caggttcctg gatcttctat gaagctcttg cccccggcgt tctgcctggc tgaagagctt
     3421 gagggtggcc aggccctgca gactctcggc aaagtaagca gacagctccc ggtacgtctc
     3481 ccatgccctt ctccccacct tccactcctc cagattggtg aatatcagca gagaaagggg
     3541 gaccaggggg acaaatgcca taagcatcag ggcggagatc agatctacat agatggcaaa
     3601 gcccaaaaag agcagaatgg gcataaccag acagagagcc atataaggaa tataaagccc
     3661 cacataggcc tcaagcgcct ccactccatc caagaccgtg gtggccaggg cgcttgtgtt
     3721 gcgcatctcg acgaaagagg gacccagatc gagaagatgc tgattgatcc tctttcggac
     3781 gctgctcttg accatggcgg cggaacgctg agaggtgata tctctggagg cctcggcaat
     3841 ggctcgcagg cttatggccg ccagcatgcc tatgaataga tccagcatgg agaggagagt
     3901 gcgaccatca tatattccca cgatgatcct tcccgccagg aagaggaggg cgatgttggc
     3961 aatagatccc atcaggccag acaagaaggt gagtgcgatc cagcgccttg caccaggggc
     4021 gaacttcaga atccgtttgt cgattaagcc ttccatgttc tccttctttg ccgcagcccg
     4081 tctcctgcgc cgtaaaatta aatcctgtcg tgttacactc gttaataaat ctgtcgtagg
     4141 agatattata aattcatatt attatcttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnng gggcagcggc cagcacatct cccgtgttgg cctttttatc tgcctgagtt
     4321 ctcatcctgt gcagtaacaa ttttcttatt ttgattatga ttaaattcta atataggttg
     4381 gattgatatg cctacatgcg acagctatct tctcctgctt gccggatgga tcacttcggc
     4441 tgcaggatgg agggcggggc agaggaaaga tggtgatgat acccttaaaa gcactaatgc
     4501 tggcctttgc attattgatg ctctctgcat cgggcaatgg ctatgtactg catatattcg
     4561 gcaatgccaa tctcgacgat gatatcaatg accaggattt agcatatcta gagaggataa
     4621 tagagggaac tgaagagccg acatggctct ctgatgcgga caataatgga gagatcgacg
     4681 aatcagacat ctcgctagta gagagcatca tcaacggaac ccctagcgag atcacaatcg
     4741 ttgactcaga caataagact gtgactgtaa agcagcccct ggagaggctg gtggtctata
     4801 cccatcagtg cgctgagatc cttcagctcc tgggagcgga ggagaaggtg gtgggggtca
     4861 gggacacatt cgctgagcag cccaacaggt tcccccggtt gagcaagtac cagagcatag
     4921 gcagtggtgg agagcccgat gttgaggcgg tcttaaagac caatcccgat gccatcctcg
     4981 cttatacctg gtacccatct gaagagtcgt tggacgataa gcttcccccg gaggttgagg
     5041 tcctgcgatt cgattgtgag tgcagcggcc agggccccga cgacatgaga gagagggtcc
     5101 ggttgctggg aatcctgatg ggtgcaaagg atagggcaga ggagtatctg cagtggcacg
     5161 acgccatcct cgaccaggtg gaggatgagg tccggaagat cccccagaat gaacgggtgc
     5221 gggtatacct ggagagcacg cccgagggcg accggcccat gagttcccga acggctattg
     5281 gcaccggcca tgcagcccac aagctggtgg agatggcagg aggtgttaac atcgctgccg
     5341 gccgccttcc tggatatgag gatacagcct tcgagtatgg cgagatagag acggagtggg
     5401 tcctctctca gaatccggag gtcatcgtag gccgggccat gggcccaggg atcaggcctt
     5461 atgaaaatgt caatgacagc ctccttgaaa gctacgccca ggatatcagg agcctgccgg
     5521 gattcgatga ggtccgggcc gtgaagaacg atgggatata catcataacc aatgactatg
     5581 caataacgcc caactacccc tctgccgtcc tccttctggc cagatggttc tatccggatc
     5641 gattcgcaga tatcgacccc caggcggctc accaggagta tgtgaatatg atgggcttgg
     5701 actatgatgt attggagaaa ggagcgttct tcttctctga agagcaagca gaggatctat
     5761 gatatttttc aactgggatt gatgaattcg acagcgacca tgagaaagga gatgctacaa
     5821 ttgagagaag ataaaatctt tgtattggcg ctggttctcc tcgccaatat cattccccta
     5881 tcatcggggt caggctatgt actccatgtt tttggcaatg ccaatatgga cggcacaatc
     5941 gactgggaag atatagccac cctcagagag atcatcagca ataatataag tcccacagac
     6001 ctggccgatg ccaacctcga tggcgaggtt gatttgcggg atatagccca ggtggagatg
     6061 atcatcaatg gaacggagag gaatctgagc cttctggacg ggaacgattt gcccatcacc
     6121 atcaataagc cagtggagag aatcgtggtc gaatacctgg acaatgccga tctgatgcag
     6181 attctcaaga agacggataa ggtagtgggg gtggacctgg cagtggccaa atccccggcg
     6241 gagtttccag agatgtccaa ccggacgtgc gtgggagcca tgcacaagga accggactat
     6301 gagaaggtat tgagtcttga tcccgatctg ctcttggtat tctccaatgt cacccaggag
     6361 aagaacaaga acctgcctgg agtgccagtg ctctttgccg gcctgtacta tcccgacctg
     6421 ctcaaacctg agacctctgc tttcaccgat gcagtgcgca agctgggcta tgttcttgac
     6481 gcccaacagg atgcagaaga gtatatccaa tggcatatga attcgctcaa tagactggtt
     6541 gagaccacag gaagtattcc tgatagcgag aggccgacgg tcctggtggc cgcctatccc
     6601 gaagctgatg agaagaccat cttcaccttt gcccagatag acacccttac cagcatggtg
     6661 gatctggccg gcgggagaac ggtggcggcc gatctgcctg attttatgaa atccacctat
     6721 agaatcgagg tggacccgga gtgggtcttg caagaagatc cggattatat cgttttgctg
     6781 gttgttgcca ctacttacag cggattggtg ttggatccgc ccagcggcta tgatgccgat
     6841 gatcccactg gaatgaagga ggctctggag gcctttatga gccgtccgga atatgccaac
     6901 ctcaaagcgg tgcagaacgg cgatgtctac atattgagcg gcaacctcag aaacgatgcc
     6961 agcaaaggcc ttatcggcgc agcctacctg gcgcggatat tccatccgga tgaggcggag
     7021 atggatcccg aggatctgca tcaggagtac ttgcagaggt tcctggggct ggactacgat
     7081 ttggatgagc acggagtatt catctatcca cccctgatca aaggtcccgg agagctggag
     7141 ggggtgccgg acggctatta tgatgctgta tcctatgaag aggctgccaa agcctgagaa
     7201 gaaagagtcg acagggcaca agccctgtcc aaatttttat caatgctggc caattttagt
     7261 aacatttttc ttttgatagt tatgattaaa tagtaaaata tgctgataag tatcattgtg
     7321 atagaaaagg gctgcttttg atcgaccgat atggtctgct ctagcatggg cgagtggcct
     7381 ggtgtaagga gactggaaat gaagggatgc ttaatagcag tgatgatgct ctgcggcctt
     7441 ttggcgcagg catcggcggg agactatatc cttcatatct atggcaacgc caatatggac
     7501 gggaccatag atgagaagga catagaatat ctccaggaga taatagcagg atcatgtaat
     7561 gcttctcccc tggcggatgc caatttcgac ggtaaggttg atgaaaatga catatcccag
     7621 gtgaagatga tcatcgatga ttctcaagag aatatcacca tacttgacgg aaatggaatg
     7681 ccggtgacgg taaggctgcc cgtggaaagg gccatcgtcg agcacctgga taattctgag
     7741 atgatgatga tcttgaaacg gacggataag gtggtaggtg tggacctggc catgtccaaa
     7801 tcggagaagg agtttcctga tctggtcaaa aagacaggcg tatccggctt ctctccagcc
     7861 acagatcccg actacgagca ggctttgagt ctgagccccg atctcctcct caccttctcc
     7921 aacaagaccg cagagaagga agagaagatg ccagaagtgc ctgttgtctt tgccggtcta
     7981 tactatccta acctgatcaa tccagagaat tcctccttta ccgatgccgt gcataagctg
     8041 gggtatatca tgcaggccag agagcaagca gaggagtaca tcgattggca tatcagcaag
     8101 atcgatgaga tcaagtcata caccgcgaat cttgccgagg aaaagaggcc tagagtgctc
     8161 atagccaccc tgccaaaaga gggagataat tcgatattca cctatgcaaa gatcgatacc
     8221 ttaagccaga tggttacttt agccggaggc agaagcatcg ccgaagatct gcccgcctac
     8281 ctggaatcaa gctacagaat cgaggtggac cccgaatggg tcattaagag cgatcccgat
     8341 tatatgatat tcatcactat cgttctcact ccgccaatag gatacgactc tgatgacatc
     8401 tcaggcattt ctcaagcact tttatctttc aagagccgcc cggagtatgc caatctcaca
     8461 gcggtcaaga acaaccaggt ctacatcatc aatggtaacc tgagaaacga tgccagcaaa
     8521 ggccttatag gggctgccta tctggcgcgg atctttcatt ccgataatgt cgatctggat
     8581 ccagaggatc tgcatcaaga gtacttgcag aggttcctgg ggctggacta cgatttggat
     8641 gagcacggag tattcatcta tccacccctg atcaaaggtc ccggagagct ggagggggtg
     8701 ccggataact actatcaaag ctatgcaaat gattctgagg caagagctca atgaggctcc
     8761 aggcaatgct gcaccattgt gctcaaattt tttaggctct tcgctgaatt atgtgggtga
     8821 gttgaggctg ggaatatggc ctctgctcct ctgtgcctct gtggttgggt cagtgcatag
     8881 cggttcacca cagagaaaca gagacacaga gaatctccga ttcaagttc
//
LOCUS       DAOSGP010000026         8813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_6771_length_8813_cov_19.824846, whole genome shotgun sequence.
ACCESSION   DAOSGP010000026 DAOSGP010000000
VERSION     DAOSGP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6771_length_8813_cov_19.824846"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1642)
                     /gene="secY"
                     /locus_tag="PLV49_01535"
     CDS             complement(29..1642)
                     /gene="secY"
                     /locus_tag="PLV49_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG97551.1"
                     /translation="MNQQSFFYAIEPFVRRLPSVERPTGHVHFKRKLSWTLAILLLYF
                     ILGNIPLFGLSAASIDLFSSYRAFFAGSFGTMMLLGIGPIVTASIVLQLLVGAEIIKL
                     NLSDPRDQAIYQGTQKALVFLMVAVEGLPQVLGGYLLPDEGVANALGVSLGIISLLIF
                     IQVFIGGSLIVYMDEVVSKWGVGSGVGLFIVAGISQQLVTGLINPATGEAGLSVGIIP
                     KWIDIIRLQLISFDTLFTSEGIRFIMITGGILALISTILIILLVVLVESTRIEIPLAH
                     SRVRGARGRFPVKLVYASVLPMILVRAIQANIEMLGALLASRLGTVSTATVTGEGVTT
                     VYTGYSSLLGNFISQSQFDAATGAAISGQSPQPVSGLMYYLSPIGGPEDWIPSMVTTS
                     TAGMAELGFSPIAGWQILLHVLTDSAFLIIGGILFAIFWIETTGMGPKSVAAKIHNSG
                     LQVPGYRRNPASIEKLMERYIPKVTVIGGVIIGVLTLIASLMGTLGGAGGTGLLLAVS
                     IVYRLYEQIASEQIQEMYPMMQKFFGASA"
     gene            complement(1639..2016)
                     /locus_tag="PLV49_01540"
     CDS             complement(1639..2016)
                     /locus_tag="PLV49_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HOG97552.1"
                     /translation="MVRPKTKEKRGHRTYHGKHKNMRGGGTRGGRGDSGKCKHHFMRS
                     ILLGTEMGKHGFVRLPLAEEVDVVNVDELDQLAGQDGKVEINELKVLGRGRVTRKLEV
                     KALGFTATAKSKIEAAGGQAVVV"
     gene            complement(2023..2478)
                     /locus_tag="PLV49_01545"
     CDS             complement(2023..2478)
                     /locus_tag="PLV49_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOG97553.1"
                     /translation="MFAIVRLRGEVNLRPDIKDTLAMLHIHRVNHCVVVKEDPHYRGM
                     IQKVKDYVAWGEIDDDTLAMLLERRGRLSANRRLTEQYLKENTPYSSFKELAKAINSG
                     SASLKDLQIKPIFRLHPARKGLRTTKKTAQQGGDLGFHQNLADLIKRMR"
     gene            complement(2481..3104)
                     /locus_tag="PLV49_01550"
     CDS             complement(2481..3104)
                     /locus_tag="PLV49_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOG97554.1"
                     /translation="MDQKKKRDFYQEEWVPKTRLGKLVYEGQVTTFDEAIASGLPIKE
                     AGLIDALIPGLEDEVLDINMVQRMTDSGRRVKFRTTVIVGNRDGYIGLGEGKDVQVGK
                     AIKKGIDNAKRNIMKVNRGCGSWECGCGQPHTVPYQVVGESGSVRVVLIPAPRGLGIA
                     AGDTSKKVMDMAGIKDVWTRTAGSTRTTINFAKATYNALINTITVRA"
     gene            complement(3109..3627)
                     /locus_tag="PLV49_01555"
     CDS             complement(3109..3627)
                     /locus_tag="PLV49_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOG97555.1"
                     /translation="MSDLATGPRYKVPLRRRKEGKTNYHVRYKLLLSKRPRVVVRKSN
                     ASTTLQLVVAELEGDKTLLTVNSRELKGFGFSSAVGNLPAAYLTGLLFGKKMLAIGVE
                     GGVADIGLHASTKGNRIYAAIKGVVDAGVDVPHSPEIFPDEERIRGEHIKSYTGKDTV
                     AQFEAAKEKILG"
     gene            complement(3620..4081)
                     /locus_tag="PLV49_01560"
     CDS             complement(3620..4081)
                     /locus_tag="PLV49_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HOG97556.1"
                     /translation="MPGFTTQRRLAASELNIGESRVWINPDPELAGALGDAITREDIR
                     SQISDGNIKAKPKKGNSRGRIRAKNAKRAYGHCKGPGHRRGAKGARSPRKELWMTKIR
                     ALRKRLRELRDTGQIDRHAYRLLYRKSKGGEYRSTAHMNSFIKAKGLARGE"
     gene            complement(4084..4458)
                     /locus_tag="PLV49_01565"
     CDS             complement(4084..4458)
                     /locus_tag="PLV49_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HOG97557.1"
                     /translation="MAERLLRVRTRQKSKKPEFNFHDSHKKKKLGTSWRKPRGLHNKL
                     RQHIAAKGRVVQPGFGSPKAVRGYHPSGMAEIRVNNVAELAAAQGCAVRIASAVGMKK
                     RLDIQAKAAEMGLKILNPKGGA"
     gene            complement(4465..4995)
                     /locus_tag="PLV49_01570"
     CDS             complement(4465..4995)
                     /locus_tag="PLV49_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOG97558.1"
                     /translation="MVKEIARQVEIPEGVTVSVDRNVVTVKGPKGELSRTLSYPDLEI
                     RKEDLQVIVNSRVDRKRHRAMVGTLASHINNMVTGVTKGYEYRMKVVYSHFPIQLKAA
                     SDELIINNFLGERKSRSARVLPGTKVEIGKDEVVITGIDKENVGQTMANIEQATRVHG
                     FDVRIFQDGIYLIDKR"
     gene            complement(5004..5393)
                     /locus_tag="PLV49_01575"
     CDS             complement(5004..5393)
                     /locus_tag="PLV49_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOG97559.1"
                     /translation="MLLDPLADAMSVIKNAESVGKPECIIHPASKVIGRALKVMADRG
                     YIGEFEFIDDGKSGMFRVKLLGRINRCGVIKPRFSTKATEMEKWEKRFLPAKNFGVLI
                     LSTNKGVMAHSDARAQSLGGQLLAYVY"
     gene            complement(5403..5555)
                     /locus_tag="PLV49_01580"
     CDS             complement(5403..5555)
                     /locus_tag="PLV49_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOG97560.1"
                     /translation="MKKGKKVYGRGANVCKRCSRKSGLVRKYGIYLCRQCFREIAPEM
                     GFEKYS"
     gene            complement(5552..6067)
                     /locus_tag="PLV49_01585"
     CDS             complement(5552..6067)
                     /locus_tag="PLV49_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOG97561.1"
                     /translation="MNVMLEPRIDKVVVHVGVGESGQRLVNAETIMKTITKQQPVRSM
                     AKKTLPTFNIKKKEPIGAKLTLRGKAAEDFLVLALKAAGNSLKMSQFDQQGNFSFGIE
                     EHTDFPGMRYDPEIGIFGMDVSVALKRAGYRIARRRVAKKKLPSRQRLGQDDTVEFIK
                     KKFGVEVLEAA"
     gene            complement(6064..6768)
                     /locus_tag="PLV49_01590"
     CDS             complement(6064..6768)
                     /locus_tag="PLV49_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HOG97562.1"
                     /translation="MSRHQKRLTIPVSWPVARKTSVWVPKSSPGPHSSQESMPLLMVI
                     RDMLKLVDNAREAKRVLYEGKVLVDGKSQKDHKLPIGIFDVIGLPLLDQQYRMLKDEK
                     GMFYLSLLEPGRARKLARIENKTILKGNKQQLNLSDGSNKLAEGDYKVGDSLVLSIPE
                     KDIDERIEFKVGNLAMVVGGKHSGQTGKIKDIIKVKSSQPNRVIISGDKEFETIEEYV
                     YMIGTDSPVIKLGAAR"
     gene            complement(6765..7124)
                     /gene="rplX"
                     /locus_tag="PLV49_01595"
     CDS             complement(6765..7124)
                     /gene="rplX"
                     /locus_tag="PLV49_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOG97563.1"
                     /translation="MITKQPRKQRKERYTAPLHRRQKYMHAPLSRALREELKKRNAQL
                     RKGDTVKVVRGDHAGTEGAVEDVDIKRCTIKVAGVSNYRADGTEVPRTIHPSNVVIVK
                     LELDDAEREKIFERRSE"
     gene            complement(7132..7530)
                     /gene="rpl14p"
                     /locus_tag="PLV49_01600"
     CDS             complement(7132..7530)
                     /gene="rpl14p"
                     /locus_tag="PLV49_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOG97564.1"
                     /translation="MRGQKAKIPRAINTGAYLDCVDNTGARTLHVISVKNYRGVKNRQ
                     PCAGIGDIVIVSVKKGTPEMRKQIFKAVIIRQRKEFRRPDGLRVKFEDNAAVIVDDEG
                     VPKGSEVKGPVAREVAERFGKIASAASIIV"
     gene            complement(7527..7847)
                     /locus_tag="PLV49_01605"
     CDS             complement(7527..7847)
                     /locus_tag="PLV49_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOG97565.1"
                     /translation="MRDIGLDVAPPEKECNDPKCPFHGTLPVRGQVFSCTVVSDKMDN
                     TVVVMRKFEKKATKYERFEKRQSKIHAHNPPCLEAKAGDRVKIAECRPLSKTKSYVVV
                     EVLK"
     gene            complement(7844..8137)
                     /locus_tag="PLV49_01610"
     CDS             complement(7844..8137)
                     /locus_tag="PLV49_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein subunit"
                     /protein_id="HOG97566.1"
                     /translation="MKLNPTNLARHELIGLNVQVESNSEPGIVGLRGRIVDETRNTFL
                     LETERKVLRIPKKNASLTITLPDGQRARVAGSVLISQPENRISKRMQRTRWKL"
     gene            complement(8134..8334)
                     /gene="rpmC"
                     /locus_tag="PLV49_01615"
     CDS             complement(8134..8334)
                     /gene="rpmC"
                     /locus_tag="PLV49_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOG97567.1"
                     /translation="MAIFKIDEIRNMNAEEISEELHKLESELIRERGVVTAGGAPEKP
                     GRIKDIRRTIARIKTVQTERRK"
     gene            complement(8340..>8813)
                     /locus_tag="PLV49_01620"
     CDS             complement(8340..>8813)
                     /locus_tag="PLV49_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOG97568.1"
                     /translation="GALGCEIVISGKLTGPRSRTEKFISGYIKHSGKPAIDLVDRGYS
                     VAVKKLGVIGCQVRIIPPDVRLPDDFQIEAPQAPPAPEPAPSAAPAPAPVPAPTPAPA
                     EEKKSELDQLLESEPEAAEVKPEPLEEPSMSAENATEIAPLSEEKAESESVLKDE"
BASE COUNT         1741 a         2629 c         2157 g         2286 t
ORIGIN      
        1 ctgtccagcg tactggacat ggattgcttc atgcggaagc tccaaagaac ttctgcatca
       61 tcgggtacat ctcctgaatc tgctcgctgg ctatctgctc atagagccgg tagacaatgc
      121 tcaccgccag aagaagacct gtgcctcccg caccacccag ggttcccata aggctggcaa
      181 tcagggtcaa gaccccaatg atcacgccgc cgatcacagt caccttggga atgtaccgtt
      241 ccattagctt ctcaattgaa gcaggattcc tcctgtaacc ggggacctgc agtcctgagt
      301 tatggatctt ggcagcgacg ctcttgggcc ccattccagt ggtctcaatc cagaatatgg
      361 cgaacagaat gccgccgatg atcaggaaag cggaatctgt gagcacatgc aagaggatct
      421 gccagccggc aatgggcgag aatccaagct ctgccatccc cgcagtagag gtggtcacca
      481 tgctgggaat ccagtcttcc ggccctccta tgggcgagag gtaatacatg agccctgata
      541 ccggctgggg tgattgtccg ctgatggcag cgccggtggc ggcatcaaac tgggattgag
      601 atatgaaatt gcccaggagg ctggagtagc cggtatagac cgtggtcaca ccctcgccag
      661 tcacagtagc tgtggagact gttccaagcc ttgatgccag caatgccccc agcatctcga
      721 tattggcctg aatagccctc accaggatca tgggaaggac tgatgcatag accagcttta
      781 ccgggaaccg gcctcttgca ccccgcaccc ggctgtgggc cagaggaatc tcaattctcg
      841 tagactccac caaaacgacc agcaatatga tcagaatggt ggatatgaga gccagaattc
      901 ctccggtgat catgataaac cggatgcctt cagaggtgaa gagtgtgtca aagctgatca
      961 gctgcagcct gatgatatca atccacttgg ggatgatccc caccgagagg ccggcttcgc
     1021 ctgtagctgg attgatcaga ccggtgacca gctgctggct gattcccgcc acgatgaaca
     1081 ggcccactcc tgatcctacg ccccatttgg acaccacctc gtccatataa acaataagag
     1141 atccgccaat aaagacctga ataaatatca ataaggatat tatgcccagg gatacgccta
     1201 acgcgttcgc cactccctcg tcaggcagga gatagccgcc cagcacctgg ggcaagcctt
     1261 caacggcaac catcagaaag accagagcct tttgggtgcc ctgatagatg gcctgatccc
     1321 ggggatcgct cagattgagc ttgatgatct ccgcgccaac cagaagctgg agcacaatgg
     1381 atgcggtaac gatgggaccg atgcccaaaa gcatcatcgt gccgaaggat ccggcgaaaa
     1441 aggcacggta agagctgaag aggtctatag atgccgcaga gaggccaaat agaggtatgt
     1501 ttccaaggat gaagtaaagg agcaatatgg ccagagtcca gcttagcttt ctcttaaaat
     1561 ggacgtgccc tgtgggccgt tccaccgaag ggagccggcg cacaaagggc tctatcgcat
     1621 aaaagaaact ttgctggttc atactactac tgcctgacca ccagcagcct cgatcttgct
     1681 cttagccgtg gcagtgaatc ctaaagcctt aacctccagc ttccgggtta ccctacctcg
     1741 accgagaacc ttcaattcat tgatctcaac cttgccgtcc tggccggcca gctggtccag
     1801 ctcatcgaca ttgaccacat ctacctcctc agctaaaggc agccggacga agccatgctt
     1861 acccatttcg gtgccaagaa ggatggatct catgaagtga tgcttgcatt tgcctgaatc
     1921 tccacggccg ccacgggttc ctcctccgcg catgttcttg tgcttgccgt ggtaggttct
     1981 atgacctctc ttttctttgg tctttggtct aaccattttt ttctacctca tcctcttgat
     2041 aagatccgcc aaattctggt ggaatcccag atctccaccc tgttgagcgg tcttcttggt
     2101 agttcttaag cccttccgag cagggtgcag cctgaatatg ggcttgattt gcagatcttt
     2161 cagactggca gagccggagt tgatggcttt ggccagttcc ttaaaagatg aataaggtgt
     2221 attctccttt aaatactgct ctgtcaggcg tcggttggcg ctcagcctgc cccttctctc
     2281 taagagcata gccagagtat catcatcgat ctcgccccag gccacgtagt ccttgacctt
     2341 ctggatcatg ccccggtaat gggggtcttc tttgaccaca acgcagtggt tgaccctatg
     2401 aatatggagc atagccaggg tgtccttgat atccggcctg aggttcacct cccctctcaa
     2461 tctcactatg gcaaacatct tcaggccctc acagttatgg tattgatgag cgcgttgtat
     2521 gtcgctttag cgaagttgat ggtggtcctg gtggagcctg cggtcctggt ccagacatcc
     2581 ttgattccgg ccatatccat gaccttcttg ctggtatctc cggccgcaat gcccaagcca
     2641 cgaggcgcag ggatgagaac tacccgaacg cttccggact cacccactac ctgatagggc
     2701 acagtgtgag gctggccgca accacattcc cagctgccgc agccccggtt caccttcatg
     2761 atgttcctct tggcgttatc gatgcccttt ttgatggcct ttcccacctg gacatccttt
     2821 ccctcgccca gtccaatgta gccgtctctg ttgcccacaa ttactgtggt tctgaacttg
     2881 accctccttc cggagtcggt catcctctga accatgttga tatccagaac ctcgtcctcg
     2941 agccctggaa tgagcgcatc tatcagtccg gcctctttta tgggcaggcc ggatgcaatg
     3001 gcctcatcga aagtggttac ctgaccctca tagaccagct ttccaagcct cgtcttgggg
     3061 acccactcct cttggtaaaa atcgcgtttc ttcttctgat ccatacactc accccagtat
     3121 cttctcttta gcagcctcga actgggctac ggtgtctttt ccagtatagc tctttatgtg
     3181 ctcccctcgg atcctctcct catccgggaa gatctcgggg ctgtggggca catctactcc
     3241 tgcatctact acccccttaa tggcggcata gatcctgttt cctttggtgg aggcatgcag
     3301 ccctatgtct gctactcctc cttccacccc aatagccagc atcttcttgc cgaagagcag
     3361 gcctgtaagg tatgctgcag gcagattgcc gacagcagag gagaagccga atcccttaag
     3421 ctcccgggag ttgaccgtca gcagggtctt gtcaccctca agctctgcca ccaccagctg
     3481 cagagtggtg ctggcattgc tcttcctgac tacaactctc ggtctcttgg atagcagtag
     3541 cttatatctg acatgatagt tggtcttgcc ctctttcctt ctgcggagag gaaccttata
     3601 tctcggtcca gttgccaagt cactcacccc gagcaaggcc cttggccttt ataaaggaat
     3661 tcatatgcgc tgtgctcctg tactctccgc ccttggattt gcggtagagc agtcggtaag
     3721 catgccggtc gatctgacca gtatcccgca gctccctgag cctctttcgc agagcccgga
     3781 tcttggtcat ccacagctct ttgcgtgggc tgcgagcgcc tttggcaccc cttctgtgtc
     3841 caggtccctt gcaatggccg taggctctct tggcattctt ggccctgatc cttccccggc
     3901 tgttgccctt cttgggcttg gctttgatgt tgccatcgct gatctgagag cgaatatcct
     3961 cccgggtgat ggcatcgccc agggcgcctg ccaactcagg atcgggattg atccagaccc
     4021 gactctcgcc gatattcagc tcagatgccg ccagccttcg ctgggtggta aatcccggca
     4081 tgttcaggcc cctcctttgg gattgaggat cttgagcccc atctccgcag ccttggcctg
     4141 aatatccagc cgttttttca tccccacggc agaggcaata cgcactgcgc aaccttgagc
     4201 ggctgccagc tctgccacat tgttcaccct aatctcagcc attccactgg gatgatatcc
     4261 tcgaacggcc tttgggcttc caaagccagg ttggacaacc cggcctttgg cagctatatg
     4321 ctgcctgagc ttattgtgca ggccacgggg ctttcgccaa cttgtgccaa gcttcttctt
     4381 tttatgtgaa tcatgaaagt tgaactcggg cttcttggac ttctgcctgg ttctgactct
     4441 tagcaatctt tctgccatcc gaattcacct cttgtctatc aggtatatcc catcctggaa
     4501 tatcctgaca tcgaagccat gcaccctggt cgcctgttcg atattggcca tggtctggcc
     4561 aacattctcc ttgtcgatgc ctgtaatcac gacttcgtct ttgcctatct cgaccttggt
     4621 tcccggcaga actcgagcag atcgagactt cctctcgccg aggaagttgt ttatgatcaa
     4681 ctcatcggaa gctgccttga gctggatggg gaagtgagag tacacgacct tcatcctgta
     4741 ctcatatcct ttagtaacgc cggtaaccat attgtttatg tgagaggcca gcgttcccac
     4801 cattgctctg tggcgcttgc ggtcgaccct ggaatttacg atcacctgaa gatcctcttt
     4861 gcggatctcc aggtcgggat aggagagtgt ccgggataac tcgccttttg gccccttaac
     4921 cgtgacgaca ttcctgtcca cggagactgt aactccttcc gggatctcta cctgtcgcgc
     4981 aatctctttg accatcagac cacctaatat acatacgcca ggagctgtcc tcccagggac
     5041 tgggccctgg catcagagtg ggccatgacc cctttgttgg tgctcagtat tagcacccca
     5101 aagttcttgg cagggaggaa tctcttctcc cacttctcca tctccgtagc cttggtggag
     5161 aaccgcggct tgatgacacc gcagcggttg atccttccca ggagcttcac ccggaacatt
     5221 cctgactttc catcatcgat aaactcaaac tcaccgatgt atcccctatc cgccatgacc
     5281 ttcagggccc tgcctatgac tttagaggcg ggatggatga tacattcagg cttgcctacg
     5341 ctctccgcat tctttataac ggacatcgca tcggccagtg gatccaataa caacgcaacc
     5401 acctacgaat acttttcaaa tcccatttca ggggcgatct ctcggaagca ctgccggcac
     5461 aggtagatac cgtactttct aactaggccg ctttttcggc tgcaacgttt gcaaacgttt
     5521 gctcctcttc catagacctt cttgcctttc ttcatgctgc ctccaagacc tcaacgccga
     5581 acttcttctt gatgaactcc acggtatcgt cctgacccag cctctgcctg cttggcagct
     5641 tctttttggc cacccttctc cgggctattc tgtagccagc cctcttcaga gctacggaga
     5701 catccatgcc gaagatgcct atctccgggt catatctcat cccgggaaag tcggtgtgct
     5761 cctcgatacc aaaggagaag ttgccctgct gatcgaactg gctcatcttc aggctgttgc
     5821 ccgcggcttt gagggcgagg accagaaagt cctccgctgc tttccctcgc aaggtaagct
     5881 tggcgccaat tggctccttc ttttttatgt tgaatgtggg cagagtcttc ttggccatgg
     5941 agcggaccgg ctgctgtttg gtgatcgtct tcatgatagt ctcagcgttc accagcctct
     6001 gaccgctctc tcctaccccc acatgcacga cgaccttgtc gatgcggggc tcgagcatta
     6061 cattcatctg gcagccccca gtttgatgac gggactgtcc gttccgatca tatagacgta
     6121 ctcctctatg gtctcgaact ccttgtctcc ggatattatg acccggttgg gctgagagct
     6181 cttcaccttt atgatatcct tgatcttccc cgtctggccg ctgtgcttcc cgcccacgac
     6241 catggccagg ttgccgacct tgaactcgat cctttcatcg atatcttttt cgggtataga
     6301 gagaaccaga gagtcgccca ctttgtagtc accttccgcc agcttattcg agccgtcgga
     6361 taggttgagc tgctgcttat tgcctttgag gatggtttta ttctcgatcc gggccagctt
     6421 tctcgctctt cccggctcaa ggaggctcag atagaacatc cccttctcgt ccttcagcat
     6481 tctgtactgc tgatcgagca gcggcaaacc gatcacatcg aatatgccta tggggagctt
     6541 atgatccttc tgggatttgc cgtccaccag aaccttgccc tcataaagga ccctcttggc
     6601 ctcgcgagcg ttatccacca gcttgagcat atccctgatc accatcaaga gaggcatgct
     6661 ctcctgagag gaatgaggac ctgggctgct cttggggacc catacgctgg tctttctggc
     6721 aaccggccag ctaaccggga tagtcagcct cttttgatgt cggctcattc agatcgcctc
     6781 tcaaagatct tctccctctc tgcatcatca agctcgagct tgacgattac cacattcgag
     6841 gggtgaatgg ttctgggcac ctcggttcca tcggcgcggt agttgctcac tcccgctacc
     6901 tttatggtgc atctcttgat gtctacgtcc tcgactgcac cttctgtgcc ggcgtgatct
     6961 ccacgcacca ccttgaccgt atcgcccttg cggagctgag cattgcgttt cttcagctct
     7021 tcacgcaggg cccggcttaa gggggcgtgc atgtactttt gacgccgatg caacggtgca
     7081 gtgtacctct ctttccgctg ctttctgggc tgttttgtaa tcatagaatc atcacacaat
     7141 tatggatgcc gcggaggcga tcttgccgaa cctctccgct acctcacggg ccacaggtcc
     7201 cttgacctcg gagcccttgg gtacgccctc atcgtccact atgacggcgg cgttatcctc
     7261 gaacttcact ctgaggccgt cgggcctgcg aaactccttc ctctggcgga tgatgacagc
     7321 tttaaagatc tgctttctca tctcaggagt tcccttctta acggacacga tcactatatc
     7381 cccgattcct gcgcagggct gtctattttt cacgcctcga tagttcttta ccgagataac
     7441 gtgtagcgtc cgcgcacctg tattgtctac acaatccagg tatgcgccag tgttgatcgc
     7501 ccttgggatc ttggccttct gtcctctcat ttcaagacct ccactaccac ataggatttg
     7561 gtcttggaga ggggccgaca ctcagctatt ttgacacgat ctccggcctt ggcttcaagg
     7621 catggaggat tgtgcgcatg gatcttggac tgcctcttct caaatcgttc gtacttcgtt
     7681 gctttctttt caaacttgcg cataactacc acggtgttat ccatcttgtc gctgaccaca
     7741 gtgcaactga agacctggcc gcgtacagga agggtaccat ggaagggaca cttgggatcg
     7801 ttgcactcct tttccggggg ggctacgtcc agtccgatat ccctcataac ttccacctcg
     7861 tcctctgcat tcttttgctt attctgttct caggctgcga gatcaaaacc gagcctgcga
     7921 ccctcgccct ctgcccgtcg ggcagggtga tggtcaggct ggcattcttt tttggaatgc
     7981 gcaagacctt gcgctctgtc tccaaaagga atgtgttcct ggtctcgtcc acaatccttc
     8041 ccctgagccc gacgatacct ggctcgctgt tggactcgac ctgaacattc aggccgatga
     8101 gctcgtgtct cgccaggttc gtggggttga gcttcacttc cttcgctccg tctggaccgt
     8161 tttgatccgg gcaatcgtcc tgcggatgtc tttaatcctc cccggcttct ccggggcgcc
     8221 accagcagtg acgacgcctc tctcgcggat gagctcactc tcgagcttat gcagctcctc
     8281 ggatatctct tccgcgttca tgtttcttat ctcatcaatc ttgaagatgg ccatttggat
     8341 cactcatcct taaggacgga ctcgctctcc gccttttctt cagaaagggg agcaatctct
     8401 gtcgcgttct ctgcagacat ggatggctct tccaggggct caggcttgac ctcggccgcc
     8461 tcgggctctg attccaggag ctgatccaat tcgctcttct tctcctcagc gggcgctggt
     8521 gttggcgcgg gcacaggagc aggagcgggt gcggctgatg gtgctggttc tggtgccggt
     8581 ggcgcctgtg gagcttcaat ctggaagtcg tctggcagcc ggacatccgg tgggatgatc
     8641 ctgacctgac agccgatgac tcccagcttc tttaccgcta cggagtagcc ccgatccaca
     8701 aggtctatgg ctggcttgcc ggaatgcttg atgtagccgg atatgaactt ctctgttcgg
     8761 gatcgtggac cggtcagctt gccgctgatc actatctcgc agcccaaagc ccc
//
LOCUS       DAOSGP010000027         8572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_7037_length_8572_cov_19.544558, whole genome shotgun sequence.
ACCESSION   DAOSGP010000027 DAOSGP010000000
VERSION     DAOSGP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7037_length_8572_cov_19.544558"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1340
                     /gene="cobQ"
                     /locus_tag="PLV49_01625"
     CDS             <1..>1340
                     /gene="cobQ"
                     /locus_tag="PLV49_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051921 - adenosylcobyric acid synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HOG97569.1"
                     /translation="SDRGYRVAPFKSQNMSLNSWVTKSGAEIGIAQAVQARAARAEPT
                     EFMNPILLKPKGDRTSQVIVLGKPLADKSAEEYYRDIEGLKEVVDRSVRELENEYDYI
                     VVEGAGGAAEINLFDRDIANIYVARLLRAPIILVGDIERGGVFASLYGTVTLLPPDVR
                     PLVRGLVINKFRGDPAILGSGLRSLEELTGVPVLGVLPYLDLDIPSEDSVSLGDKKAR
                     FSSEAVEIAVIRLPRISNFTDFEPLERRAYIRYVDLDEPLGHPDAVIIPGTKNTISDL
                     LEMEKKGMADQIRSLQGTPILGICGGYQILGKEIIDCGVEDTEGSMPGLGLLNATTRF
                     DQYEKRTVQVRKPVTGGGPILDRIRGQEVTGYEIHMGVTASMDPAAFGDDGAVASSGL
                     VIGTYLHGIFDNQNLRDAFLDFLYERKNSTGIKASGLKAEGKSHRANAQKGSGY"
     assembly_gap    1341..1440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1441..2043
                     /locus_tag="PLV49_01630"
     CDS             <1441..2043
                     /locus_tag="PLV49_01630"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOG97570.1"
                     /translation="LITLLSQNPDSYMFHTPNICHTHFQAEAMHIPQLCRPTQGVKEK
                     ELEDLKSALAEARDCYGVQGIVTGAIESVYQAARVQRICHSLDLWCFNPLWQTDQIDY
                     LKTLLTEGFRVIISGVYAYPFDASWLGAELTEEKIMRLAQLQERYQINPSGEGGEIET
                     FVLDGPTPLFRKRIEILRASGTYANYRGQYIIEEMRLVEK"
     gene            2040..2612
                     /locus_tag="PLV49_01635"
     CDS             2040..2612
                     /locus_tag="PLV49_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97571.1"
                     /translation="MNGKAKILLVDSCYEADSLSQYEFVRPVQRLLERTGFPCQICHI
                     SLLDEERLKGCDKVVLCGTALKDNEYLNHLERYTWLKEGDWPVLGICAGMQIIAAIFG
                     GEVRSRHKPAIGLEKIEVVRVSDILGPSRKIEVYHIHSLDVTLPEGFISLAGTELSPE
                     AFSHCSLPIWGLLFHPEVRNRWILENYANL"
     gene            2682..3866
                     /locus_tag="PLV49_01640"
     CDS             2682..3866
                     /locus_tag="PLV49_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HOG97572.1"
                     /translation="MQDYSLNQFLSLAGTPPLDIEICDVTLRDGEQMPGVVFRADEKL
                     DIALRLDEIGVEVIEAGFPVVSAAEMNAVKEVCNLGLKAKISALSRSVKKDVDAAIDC
                     GVDMISVFIATSDLHLKHKLHMSCPEAIACALQTVEYAKDHGLIVRFSAEDATRTDFE
                     TLKKLYKKAEEYHADYVSVADTVGIMNPRTTHYMISELRKSIKTPICMHCHDDLGMAL
                     ANTLAGAEAGARQLHTTVNGIGERSGNTPLEELLIALRLHYDIESYDLSKIKDLSQLV
                     QTYSDVPVAKNKAVVGTNAFSHESGIHVAAVLEEPRTYELYSPELVGAARHIIIGKHT
                     GVKALKYITQRMGYYLKDQELGDLSERVKMCSEYKHAINCEELRKLILNMENIEVIYP
                     GP"
     gene            4001..5053
                     /locus_tag="PLV49_01645"
     CDS             4001..5053
                     /locus_tag="PLV49_01645"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOG97573.1"
                     /translation="MKALILSGGSGTRLRPLTYSQQKQLIPVANKPVLFYALEDVIEA
                     GADEIGIILGPNKEQVIETVQSADWEVPIKFIYQGEPKGLAHTILVAEDFLDEDFVMY
                     LGDNILRDGIVSHEKRFHSLGSSASVLLTPVDDPQRFGVADLNPDGSIRRLVEKPKVP
                     PSNYALVGVYFFTPLIIEACKSIKPSWRNELEITDAIQWLIENGHKVDASFVEGWWKD
                     TGKPEDIFEANRLILDDIDGRCDGEVEESRIMGRVVMEEGTRIRRSVIKGPCIIGRDS
                     IISDSYIGPYTSIGIGCQICGTEVEDSIIMDESRIVNAGKVVESLIGRNVRIKECDSL
                     PRGSRMIVGDNSDISL"
     gene            5059..6060
                     /gene="rfbB"
                     /locus_tag="PLV49_01650"
     CDS             5059..6060
                     /gene="rfbB"
                     /locus_tag="PLV49_01650"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOG97574.1"
                     /translation="MNLLVTGGLGFIGSNFIRLMLNRHDDCRILNLDAQGFGSNIQNL
                     ADYEDDRRYTFFRGDIADSSLVSSLVEKADLVVNFAAETHVDRSISRPDSFLHSNVNG
                     VFCLLEAIRDHNPSVRYVQISTDEVYGDILRGSSTEDSTLRPSSPYSASKAAGDVFVL
                     AYARTYGLEAMITRCTNNYGPYQFPEKLIPKTIIRAKEGLKIPIYGTGENVRDWIYVT
                     DHCLAVEQVLNRGRRGEIYNISAGEERTNLFIAKFILEMLGKSEDQIEFVEDRPGHDA
                     RYSLDSSRIRKELGWRPERSFEEGLQTTVEWYLQNPDWYGPLVNDSVLSATPWRLKW"
     gene            6073..>6790
                     /locus_tag="PLV49_01655"
     CDS             6073..>6790
                     /locus_tag="PLV49_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG97575.1"
                     /translation="MRIFITGGSGLAGSKLAEMALARGEQVYSGYAHNQPPYGREVKF
                     DLLDANGIRDIIERMRPDVIVHSAALTDVDRCEREKDLAYKINVEGTRAIAEAARKAG
                     SYLVYISTDYVFDGQRGLYREEEETNPVSYYGLSKLLGEQFCLDQGCIARTCVIYGSR
                     PASGKVNFALWLLNALKSGKEARVVTDQFITPTLNSNLAAMVLEAADRHLSGFYHLSG
                     ASRVSRYDFACELARAFDLDR"
     assembly_gap    6791..6890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6891..7354
                     /locus_tag="PLV49_01660"
     CDS             <6891..7354
                     /locus_tag="PLV49_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOG97576.1"
                     /translation="FADERGFFTEIMRTDWKDVIQDDIVQANMSLSYPGMVRAWHRHE
                     RGQVDHFLVVRGALKICAYDDDTRELDEIVSAGDNPQMVRMPGHYWHGFGVVGNEQAT
                     LIYFVNRLYDYASPDEERRPWNDPAIVPDTINGKKDERCDRTWDWFLPAFK"
     gene            7361..>8572
                     /locus_tag="PLV49_01665"
     CDS             7361..>8572
                     /locus_tag="PLV49_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOG97577.1"
                     /translation="MNTIQRIAKNTIFLASSNIIGFVFSFFFLVYTTRYLGTERYGLL
                     SFAIAFASITIFLADIGISSVIVRDVARDRSKTAIYIGNSILMKVVLAAITLLSTAAI
                     GFALGYPFSTMKVVMVILLSLIVISFSGIISSVVSAYEIMEYISIGTVIYNLIMLTFA
                     LLAISSGVDVYGFAYVYLFSSFISLGYFALVALRKLPRPRWDIDISLWKYMIKEAIPF
                     GLSSVFVRVYYYVDTVMISLLILNPNEVMGWYNAAYRMVIILSFIPVTFLGSIYPIMS
                     KFYVSSGQYLGFMYERSFKYLMAIAIPIGVGTTVLGEDLVSLVYGPAFAPSTIALQIL
                     IWSEALIFINSAFGYLFNSINRQMIVAKQTMLAAGLNILLNLFLIPPYSYIGASSATV
                     ATQLFSFLFLLH"
BASE COUNT         1978 a         2111 c         2385 g         1898 t
ORIGIN      
        1 tccgaccggg gataccgcgt agctcccttc aagtcccaga acatgagcct caactcctgg
       61 gtcacaaaaa gcggtgcgga aatcggcatc gcccaggcgg tgcaggcccg cgccgccagg
      121 gccgagccca cggagttcat gaaccccatc ctcctcaagc ccaaggggga tagaacatcc
      181 caggtcatag tcctgggaaa gcccctggcc gacaaatcgg cagaggagta ctaccgggac
      241 atcgagggtc tgaaagaggt ggttgaccgc tcggtaagag agctggagaa tgagtacgac
      301 tacatagtcg tggagggggc aggaggagca gcggagatca atctcttcga ccgggatatc
      361 gccaacattt acgtcgcccg gctgctccgg gcccccatta tcctggtggg tgatatcgag
      421 cggggagggg tcttcgccag cttatacggc acagtgacgc tcctgcctcc ggatgttcgc
      481 cctctggtcc ggggcctggt gatcaacaag ttccggggcg atcccgcgat cctcggatct
      541 ggtttgaggt cgctagagga actcacaggc gttcccgtat tgggggtcct gccctatctc
      601 gatttggaca taccatctga ggactctgtc tccctgggcg acaagaaggc ccgcttctcc
      661 tcagaagcgg tggagatcgc ggtgatcagg cttccccgga tcagcaactt cacagacttc
      721 gagcccctgg agaggagggc ttatatcaga tatgtggatc tggacgagcc cctgggccat
      781 ccggatgcag tgatcatacc gggaaccaaa aacaccatat ccgacctttt ggaaatggag
      841 aagaagggca tggcggatca gatcagatcc ctgcagggga ctccaatcct cggcatctgc
      901 gggggctatc agatcctggg aaaggagatc atcgactgcg gggtagagga taccgaaggc
      961 agcatgcccg ggctgggatt gctcaatgcc actacccgct ttgaccagta tgagaagagg
     1021 acggtgcagg tgagaaagcc agtcactggt gggggcccaa tactggatag aatcagaggc
     1081 caggaggtca caggctatga aattcacatg ggggtgacag cttccatgga tccggctgcc
     1141 tttggagatg atggcgcagt ggcgagcagc ggcctggtga taggcaccta tctgcacggc
     1201 atctttgaca accagaactt aagggacgca ttcctggatt tcctctatga gagaaagaat
     1261 tcaactggga tcaaggcgtc aggcctaaag gcagagggca agtctcatcg ggcaaatgcc
     1321 caaaagggca gtggctacag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 ctgataaccc ttctctctca aaatccggac agctatatgt tccacacccc aaacatctgc
     1501 catacccatt ttcaggccga ggctatgcac atcccccagc tctgccggcc cactcagggg
     1561 gtaaaggaga aggagctgga ggatctgaag tccgccctgg ccgaggcgcg ggactgctat
     1621 ggcgtccagg ggatcgtcac cggggcgatc gagtcggtct accaggcggc cagagtgcag
     1681 aggatctgcc attctcttga cctgtggtgc ttcaatcccc tctggcagac cgatcagatc
     1741 gattatctta agactctctt aactgaagga tttcgggtga tcatctctgg ggtctatgcc
     1801 tatccctttg atgccagctg gctgggagcg gagctcacag aggagaagat catgaggctc
     1861 gcccagctgc aggagaggta tcagatcaat ccctctggcg agggagggga gatcgagacc
     1921 tttgtcctag acggccccac ccccctcttt cgaaagagga tcgagatctt gagagcatcc
     1981 gggacctatg ccaactaccg tggccagtac atcattgagg agatgaggct ggtggaaaaa
     2041 tgaatggcaa agcaaagatc ctcctggtgg acagctgcta cgaggccgat tccctctctc
     2101 aatatgagtt cgtccgtccg gttcaaaggc ttctggagag gacaggcttt ccctgccaga
     2161 tctgccatat ctccctgctg gatgaagagc gtctgaaagg ctgcgataag gtggttctct
     2221 gcggcactgc cttgaaggat aacgagtacc tgaatcatct ggagagatac acctggctga
     2281 aggaaggaga ctggcccgtc ctggggatct gtgccggcat gcagatcatc gccgccatct
     2341 ttgggggaga ggtcagatcg cgacacaagc ccgccatcgg cctggagaag atcgaggtgg
     2401 tccgggtgtc ggatatcctc ggccccagcc gcaaaatcga ggtctatcat atccatagcc
     2461 ttgatgtcac cttgcccgag ggattcatca gtctggcagg gacagaattg tctccagagg
     2521 ccttctccca ctgctcactc cccatttggg gcctcctatt tcatcccgag gtgcgcaacc
     2581 gctggatcct ggaaaattat gcgaatctct aactctgaac ttgccggaaa gctcattcct
     2641 tcgaaaccaa tttatgtaat gaatcctcat ttaaccgcat tatgcaagac tattccctta
     2701 atcaatttct cagtctggcc gggactccgc ctctggacat agagatctgc gatgtaactt
     2761 tgcgcgatgg ggagcagatg ccgggagtgg tctttagggc tgatgagaag ctggatatcg
     2821 ctctccggct ggacgagatc ggcgtggagg tcattgaggc gggctttcct gtggtctctg
     2881 ccgcagagat gaacgcggtg aaagaggtct gcaacctggg gcttaaggcc aagatctctg
     2941 ctctctcccg atcggtcaag aaggatgttg atgctgctat tgactgcggc gtggacatga
     3001 tcagcgtctt catcgccacc tcggaccttc atctcaagca caagcttcac atgagctgtc
     3061 ctgaggccat agcctgtgct ctccaaacgg tcgagtacgc caaggatcac ggcctgatag
     3121 tgcgcttctc agcggaagat gcaacgcgca ccgattttga aaccttaaag aagctgtaca
     3181 agaaggcgga ggaataccat gcagactacg ttagcgtcgc ggatactgtg ggcatcatga
     3241 acccccgcac gacccactat atgatcagcg agcttcgcaa gtccataaag accccgatct
     3301 gcatgcactg ccatgacgac ctggggatgg ccctggccaa cacccttgct ggggcagagg
     3361 cgggggccag gcaattgcat accactgtga acggaattgg cgagcgcagc ggcaatactc
     3421 ccttagaaga gctcttgatc gccctgaggt tgcactacga tatcgagagc tatgatctct
     3481 ccaagataaa ggacctctcc cagctggtcc agacctattc cgatgttcca gtggccaaga
     3541 acaaggcggt tgtggggacc aatgccttct cccacgagtc agggattcac gtggctgcgg
     3601 tgttagagga gcccaggacc tacgagctct actccccgga gttggtgggc gcagcaaggc
     3661 atatcatcat cggcaagcat accggagtca aggccctgaa gtacatcacc cagaggatgg
     3721 gctactatct caaggatcaa gagctaggcg acctatctga gagggtgaag atgtgcagcg
     3781 agtacaagca tgccattaac tgcgaagaat tgagaaagct gatactgaac atggagaaca
     3841 tcgaggtcat atacccgggt ccttgaattg gctgccaggc caatcggatt tgcaccactt
     3901 ttatcaatga tctattcagc agatcgaagg gtatatcttg ccgttatgta caactctgaa
     3961 tccatggatg cagaattaag ggctttttga ggtgtgaact gtgaaggcgc tcatactttc
     4021 aggcggctca gggacgagac tgcgacctct gacctattcg cagcaaaaac agctcattcc
     4081 cgtagccaat aaaccagttc ttttctatgc cctcgaggat gtgatcgagg ccggggcgga
     4141 cgagatcggc ataatcctgg ggccgaacaa ggagcaggtc atagagacag tgcagtcagc
     4201 ggactgggag gttcccatca aattcatcta tcagggtgag cccaagggac ttgcccacac
     4261 catcctggtg gcggaggatt tcctggatga agattttgtt atgtacctgg gggacaatat
     4321 cctaagagat ggcatagtca gccatgagaa gcgttttcat agtctgggca gcagcgcaag
     4381 cgttctgctc accccggtcg acgatcctca gcgcttcggt gtggccgacc tgaacccgga
     4441 cgggagcatc aggcgcctgg tggagaagcc caaagtccct ccctccaatt atgccctggt
     4501 gggggtgtac ttcttcaccc cattgatcat agaggcctgc aaatccatca aaccatcctg
     4561 gaggaatgaa ctggagatca ccgacgccat tcagtggctg attgagaatg gccataaggt
     4621 ggatgcctcc tttgtggagg ggtggtggaa ggataccgga aagccagagg acatattcga
     4681 ggccaatcgc ctgatcctgg acgatatcga tggaaggtgc gatggcgagg tggaggagtc
     4741 ccggatcatg ggacgggtgg taatggaaga gggaaccaga atcaggaggt cggtgatcaa
     4801 ggggccctgc atcatcggaa gggacagcat tatatccgat tcatatattg gcccttacac
     4861 ctcaattggc atcggctgtc agatatgcgg cacggaggta gaggactcga tcatcatgga
     4921 tgaaagcagg atagtcaatg ccggaaaggt ggtagaaagc ctgatcggca ggaacgtcag
     4981 gatcaaggaa tgcgatagcc tgccaagagg aagccggatg atcgtgggag ataactccga
     5041 tatatcgctg tgatcgctat gaatcttctt gttaccgggg gccttggctt tatcggcagc
     5101 aacttcatcc gattaatgct gaaccggcat gatgactgca gaatcctgaa cctggacgcc
     5161 caaggtttcg gctccaacat ccagaacctg gcggactatg aggatgacag gaggtacacc
     5221 tttttccggg gagatatagc cgatagcagc ctggtatcca gtctggtgga aaaggcggat
     5281 ctggtggtga acttcgctgc tgagacccat gtggaccggt ccatctcccg gcccgactcc
     5341 ttcctgcata gcaatgtcaa tggggtcttt tgcctgctgg aggccataag agatcataat
     5401 ccttccgtca gatatgtcca gatctcaacc gacgaggtct atggggacat cctcaggggc
     5461 tcatccacag aggattcgac attgcggccc tcatccccct attccgccag caaagcggca
     5521 ggggacgtat tcgtcctggc ctatgcccga acctacggtc tggaggccat gatcacccgc
     5581 tgcaccaaca attatgggcc ctaccagttt cccgaaaagc tgattcccaa gaccataata
     5641 agggctaagg aagggctgaa gatccccatt tacggcacag gagagaatgt gagagactgg
     5701 atttatgtga cagatcactg cctggcggtg gagcaggttc tcaatagggg gagaagaggc
     5761 gagatctata atatctctgc cggagaggag aggaccaatc tgtttattgc caaattcatt
     5821 ctcgagatgc tgggcaagag cgaggatcag atcgagtttg tggaggatag gccgggacac
     5881 gatgcccgct acagcctgga ctcgtcccgg atcagaaagg agctgggctg gcggcctgag
     5941 cggagctttg aggaagggct tcagacgaca gtggaatggt atctgcagaa ccctgattgg
     6001 tacggcccgc tggtgaacga ttcggtattg agtgccaccc cctggcggct gaagtggtag
     6061 gggcgatatt ccatgagaat cttcataacc ggcggcagcg gccttgcggg aagcaagctg
     6121 gcagagatgg ccttggccag gggagagcag gtctatagcg gatatgctca taaccagccc
     6181 ccctatggaa gagaggtaaa atttgacctc ttggacgcaa atggcatccg cgatatcatc
     6241 gagaggatga ggccggacgt aatcgtgcac agcgccgccc tgaccgatgt ggatcgatgt
     6301 gaacgggaga aggatttggc ctacaagatt aatgtggaag ggacccgggc gatagcagag
     6361 gcggcgagaa aggccggatc ttatctggtc tacatatcga cggactacgt cttcgatggc
     6421 cagcgggggc tgtaccggga ggaggaggag acgaacccgg tgagctatta cggcctttcc
     6481 aagctcctgg gtgagcagtt ctgtctggat caggggtgta ttgccaggac ctgtgtgatc
     6541 tatggcagcc ggccggccag cggcaaggtc aacttcgctc tatggctgtt gaacgccctt
     6601 aaatcaggaa aagaggcgag ggtggtcaca gatcagttca taacccccac cctgaacagc
     6661 aatctagcgg caatggtgct ggaggcggcg gaccggcatc tatccgggtt ctatcatctc
     6721 tcaggagcct ccagggtatc cagatatgat tttgcctgcg agctggccag ggcattcgac
     6781 cttgatcgcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gattcgccga
     6901 tgagcgtgga tttttcactg agatcatgag gacggactgg aaggatgtta ttcaggatga
     6961 cattgtgcag gccaatatgt ccctgagcta tccgggcatg gtaagggcct ggcaccggca
     7021 tgagagagga caggtagatc atttcctcgt agttcgaggc gcgcttaaga tctgcgctta
     7081 cgatgatgat acccgggagc ttgatgagat cgtctccgcg ggagacaatc cccagatggt
     7141 caggatgcca gggcattact ggcatggctt tggggttgtg ggaaatgaac aggcgacgct
     7201 gatttatttc gtaaaccggc tttatgatta tgccagtccc gatgaggaga gaaggccctg
     7261 gaatgatccg gctatcgttc cggatacgat caatggcaaa aaggacgagc gctgcgatag
     7321 aacatgggat tggtttcttc cagcgttcaa gtgatttgca gtgaatacca ttcagaggat
     7381 agccaagaac accatatttt tggcctcctc aaatatcatt ggatttgtat tcagcttctt
     7441 ctttttggtc tacaccaccc gttatctggg aacggaaagg tatggcctgc tctcttttgc
     7501 catcgctttt gcctcgatta ccatcttcct ggcggatata ggaatctcct cagtgatcgt
     7561 cagagatgta gcccgagata ggtccaagac ggcaatatat attggaaact ccatattaat
     7621 gaaggtcgtc ctggctgcga tcacccttct gagcacggcg gccatagggt ttgccctggg
     7681 ctatccgttc tccaccatga aggtagtgat ggtgatcctc ctctctctca tcgtcatctc
     7741 cttttccggc atcatcagct cagtcgtttc cgcttatgag ataatggagt acatctcaat
     7801 aggcacggta atatataatt taattatgct caccttcgcc ctcctggcca tcagttcagg
     7861 ggtagatgtc tatggctttg cctatgtata cctcttctca agcttcatct ctctgggcta
     7921 ttttgccctt gtggctctga gaaagcttcc ccggcccagg tgggatatag atataagcct
     7981 ctggaaatat atgataaagg aggccattcc cttcggccta tcctcagtct ttgtgagggt
     8041 gtattattat gtcgatacag ttatgatatc tttattgata ttaaatccca atgaggttat
     8101 gggatggtac aatgcggcat atcgtatggt aattatcctt tcctttattc cagtgacttt
     8161 tttgggatca atatatccta ttatgtcaaa attttatgtc tcttcaggcc aatatttggg
     8221 attcatgtat gagcgatctt tcaagtatct gatggcgatt gccattccca ttggagtggg
     8281 aacaacggta ttaggagagg atttggtctc ccttgtgtac gggccggcct tcgctccctc
     8341 aacaattgcc ctgcagatat tgatctggtc tgaggcattg atcttcatca attcagcttt
     8401 tgggtatcta ttcaattcca ttaaccgcca gatgatcgtg gccaagcaga ccatgctagc
     8461 cgctggattg aacatcctct tgaatctgtt tctgatacct ccgtacagct acataggagc
     8521 gagcagtgca acggtggcca cgcagctctt ctcatttctc ttcctcttgc ac
//
LOCUS       DAOSGP010000028         8391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_7252_length_8391_cov_19.275672, whole genome shotgun sequence.
ACCESSION   DAOSGP010000028 DAOSGP010000000
VERSION     DAOSGP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7252_length_8391_cov_19.275672"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLV49_01670"
     CDS             complement(<1..443)
                     /locus_tag="PLV49_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOG97578.1"
                     /translation="METPVPVRVKGVYIAESEESNPAPVVLLEDEKGRIVPIFVGLSE
                     AISIHHALSGELAPRPMTHDLFISALESLSASITNALIDDLDGGIYYARLTIKSDSRK
                     NEIDARPSDCLALALRAKASIEVRERVVADASISKSDAEKLISIE"
     gene            complement(493..3654)
                     /gene="ileS"
                     /locus_tag="PLV49_01675"
     CDS             complement(493..3654)
                     /gene="ileS"
                     /locus_tag="PLV49_01675"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOG97579.1"
                     /translation="MISEVAGQYNAQDLENEVRSLWERADSYRKVRELRLGGKKFFFV
                     DGPPYTTGRIHLGTAWNKVIKDAVLRYRSMNGFEVKDRAGWDMHGLPIEVKVEESFGF
                     RNKKDIEAYGVDKFIQRCKEFALRQKDDMTAQFKILGAWMDWDDPYMTLKNEYLEAAW
                     WTLKKAHEHNLLERGSRVVNWCPRCQTAIADSEVEYWDETDYSIYVKFPVIGEENTYI
                     VIWTTTPWTIPANVAVAVNPSFQYSRVRAWKDGKSEILIMASELVDSVLRTGRYQDYE
                     LLQGLSAKDMQKLKYEHPLLDLVPRQREIEHGVYAADFVTAENTGCVHIAPGHGLEDY
                     ELGLDHHLEIFCPVGGDGRYTAEAGEKYLGQYVKEADSNVIADLEERDKLLAQGRLAH
                     RYGHCWRCKTPIIFIATSQWFLRISDLRDKMLDEISRVTWYPEWAGSARFADWIRNAR
                     DWCISRQRYWGIPLPIWTCPDCGHMEVIGTASELEERSGKKIADLHRPDVDAIVLDCP
                     CGGKMLRSPDVFDVWFDSAVASWATLRFPSHEEEFGEWWPADFITEGHDQTRGWFYSQ
                     LGASMVSFGRAPYKSVLMHGFTLDDQGRKMSKSIGNIVQPEEVVERFGADILRFYVLG
                     ANAPWEDLHFSWEAVENTSRMINIFWNAYRFALPYMVLDDFDLKRADLDLYKSSLRPE
                     DRWILSRVNSLAEEITAEMEGYQLHRIVRSISDFILEDLSRWYIQLVRPRTWIEQDDP
                     DKLAAYATIYEVLTKLARLMAPFTPFIAESIYQNLVRGLDENARPSVHMCDWPEGDKG
                     LIDKRLEESMDLVREVSMAASNARQKGGRKLRWPVSEIVIAPSKEALDLGGLEDVLRG
                     QTNSKKITVLSPGEKPRMDLEIAPVHKKIGPVYKGEAKLVVEAIAAAEPFGVKADLDG
                     SGQATLRHQGREFAITSDMVQFRELPPENLPAAEFSRGFVYVDIALTPELEAEAYARE
                     IIRRIQDMRKELDLRVEDQIRAVVDIESKTILDLVVSGKEHIAGEVRAAELEMGLGLQ
                     IRGSLIKDWDVEGVCMHIGIDRV"
     gene            3802..4755
                     /locus_tag="PLV49_01680"
     CDS             3802..4755
                     /locus_tag="PLV49_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HOG97580.1"
                     /translation="MWIEKYRPERLDDIVGQDEIVRRLKSYVKTRNLPHLLFSGPPGV
                     GKTAASISIVKEIFGETWRNNFIELNASDERGIDIIRHKVKDFARMAPLGEADFKVIF
                     LDEADALTNDAQSALRRTMERYSATTRFILSCNYSSKIIEPIQSRCAVYRFKPLSTEA
                     VTKRIKFIASEEGLRVSDGGLSAIEYVAGGDMRKAINALQAAALLGDEVDEETIYQIT
                     STAKPEEIKSFIKTAISGDFVGARAILDDLLLSKGLSGQDVVIQIHRAMLDLDDIADR
                     DRVKLIDRIGEIDFRMTEGANERIQLEALLAYFALMASEQR"
     gene            complement(4801..>6005)
                     /locus_tag="PLV49_01685"
     CDS             complement(4801..>6005)
                     /locus_tag="PLV49_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG97581.1"
                     /translation="TERKKVEQDLREAKDRAESATKAKSEFLANMSHEIRTPMNAVIG
                     FTSLLLNTNIDSVQRDYIETIRSSGDSLLKVISDILDFSKIEGGMMELEKERIDLIEC
                     LETSLNMVAEDASRKGLSVSYEVEPQVPRYLIGDMTRLRQILVNLLGNAVKFTDKGFV
                     RLDVSALPVEDGHEIQFRVTDSGIGISQDRMSRLFQSFSQVDASTTRKYGGTGLGLAI
                     CKHLAELMGGGIWAESVPGEGSSFYFTIQAEASIEPYPKLHGDRPDLTLDLSDQEIQK
                     GLRILLAEDNLVNQKVAIRMLERLGYTADIAADGREVLAALESRPYDVVLMDVQMPEM
                     DGLEATRSIRITACHQPYIIAMTAHAMKGDREECLDAGMNDYISKPVRIEELKAALMH
                     SRMPVFNG"
     assembly_gap    6006..6105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6106..7811)
                     /locus_tag="PLV49_01690"
     CDS             complement(<6106..7811)
                     /locus_tag="PLV49_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG97582.1"
                     /translation="MNPERMFQSTLEILEDGICICDPSGEILYSNPAAERMARSLEEL
                     NCGKGGVLKDLCSGILKTAKSADGPIHRGYVDLQRRRIEYSIATMQGKGYEGRSIITF
                     REAHPSSGQEYDDSPPSENAIFWIEHQGRNDDLRERDRIMAGAALATNQLLISREMDV
                     ALNLSLEILGCSANVDRACIFEHYLDEEGRDQISLGYEWISGNDGDAGSAKIRHPDLS
                     CKAYSSIPQWFQILSGGMPIRGRTRDLPPPARGPLKMMGVRSFMIVPIFTTDRFWGFI
                     GFEDHRLERDWSWSAAFILMTMASAIGGFIGRFIAESALRESEQKYRELVETSSSVII
                     RVDTSGAIRFINKFGLHFFGYNEDDILGRNVSETIFPPGGKGCNLDGIIRHIREHPEE
                     CSSHIAGNMKSSGEPVWIAWTHRLVQNERGEVAEILCIGNDITEKKRSSEEFKRVAAD
                     LRETRDYLDNLLSHANAPIIVWDPNFCITRFNHAFERLTGWRADEVLGRSLGILFPDE
                     SRTESYAYIERTLSGESWNAVEIPICHQNGEVRTVLWNSANIYDETGRRVIATIAQGQ
                     DIT"
     gene            complement(7801..>8391)
                     /locus_tag="PLV49_01695"
     CDS             complement(7801..>8391)
                     /locus_tag="PLV49_01695"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOG97583.1"
                     /translation="GDSPFSRQWMAGLGKGSNYSLSVLSLGSHTGTHIDFPSHILRDG
                     FPLDSYPPERFITPARVICVRNCDAVPAQALHDVNIQRGEAILFKTSNSFQGLMHNPT
                     FRDEYVSLSPPAAELCVSLGAALVGIDYISVDRYEDESLPVHNILLKNDVLILEGIDL
                     SAVSPGRYWLICLPLKIKDAEAAPVRAVLVGRSHES"
BASE COUNT         1786 a         2485 c         2147 g         1873 t
ORIGIN      
        1 ttctcgatgc taatcagctt ctcagcatcg ctcttgctga tgctcgcatc cgccaccacc
       61 ctctcccgaa cctcgatcga tgccttcgcc cgcaaggcca gagcaaggca gtcgctgggc
      121 cgggcatcta tctcattctt tctagagtcg ctcttgatgg tcagccgtgc atagtagatt
      181 cccccgtcca ggtcgtcgat cagcgcattg gtgatgctgg cggacaggct ctccagggcg
      241 gagataaaca gatcgtgagt catgggcctg ggggccagct ctccagacag ggcatgatgg
      301 atggagatgg cctcggacag ccccacgaat atgggcacta tccttccctt ctcatcctcc
      361 agcaaaacca caggcgcggg attgctctcc tccgattccg ctatgtaaac ccctttgacc
      421 ctgaccggga ctggcgtttc catgagatcc tcatgatcgt tcatcttata aaactccgac
      481 gatcaatatc catcacaccc tgtctatgcc gatgtgcatg catacgccct cgacatccca
      541 gtccttaatc aaagagcccc gtatctgcag tcccagaccc atctccagtt ctgccgccct
      601 aacctctccc gcgatgtgct ctttccctga aactaccaga tcgagtatgg tcttgctctc
      661 gatatccacc accgctcgga tctgatcctc tactctcaga tccagctcct ttctcatatc
      721 ctgaatgcgc cggatgatct ccctggcata ggcctctgcc tcaagctccg gggtgagggc
      781 aatgtccaca tatacaaatc cccgggagaa ctccgctgcc ggcaggttct ccggaggaag
      841 ctcccggaat tgcaccatat ctgaggtgat cgcaaactcc cgaccctgat gcctcaaggt
      901 cgcctggccg ctgccgtcca gatcggcttt aaccccaaag ggctcagcgg ctgctatggc
      961 ctctaccacc agcttggcct ctcccttata cactggcccg atcttcttat gaacgggagc
     1021 aatctccaga tccatcctgg gcttctctcc tggagagagc acagtaatct ttttggagtt
     1081 ggtctgcccc cggaggacat cctccaggcc tcccaggtcc aaagcctctt tggagggggc
     1141 gatcacaatc tcagaaaccg gccagcgtag ctttctcccg cccttctgcc gggcattgga
     1201 ggcggccata gacacctccc tcaccagatc catgctctcc tccaggcgtt tgtctatcag
     1261 tcccttatcc ccttctggcc agtcgcacat atgaactgaa ggcctggcat tctcgtccag
     1321 accccgcaca aggttctggt agatggactc ggcgatgaag ggggtgaacg gagccataag
     1381 cctggccagc ttcgtcagca cctcatagat ggtagcatat gcagcaagct tgtccggatc
     1441 atcctgctca atccaggtgc gtggccggac cagctggatg taccagcgac tcaggtcctc
     1501 taagatgaag tcggatatgg agcggacgat gcggtgaagc tgatagccct ccatctccgc
     1561 ggtgatctct tccgccaggc tgtttacccg ggagaggatc cagcggtcct cgggacgcag
     1621 gctgctcttg taaaggtcca gatccgcccg tttcaagtcg aaatcgtcca gaaccatata
     1681 aggcagggca aagcgataag cattccagaa gatattgatc atccggctgg tgttctccac
     1741 cgcctcccag gagaagtgca gatcctccca gggggcattg gctcccagga catagaaacg
     1801 cagaatatcg gcaccgaacc gctccaccac ctcctccggc tggacgatgt tcccaatgct
     1861 tttggacatc ttccgaccct ggtcgtccag ggtgaagcca tgcatgagaa cgctcttgta
     1921 gggcgcccgg ccaaatgata ccattgaggc ccccagctgg gagtaaaacc agccgcgtgt
     1981 ctggtcgtgc ccctcggtga tgaaatccgc cggccaccat tcgccaaact cctcctcgtg
     2041 gctggggaaa cggagggtgg cccaggaggc cactgcgctg tcaaaccata catcgaagac
     2101 atccggtgag cgcaacatct tcccaccgca gggacagtcc aagacaatag catccacatc
     2161 cggccggtgc agatcagcga tctttttgcc tgacctctcc tccagctccg aggcggttcc
     2221 tataacctcc atatggccgc agtcaggaca ggtccagatg ggcaggggaa tgccccagta
     2281 cctctgcctg gagatgcacc agtctcgggc attgcggatc cagtcggcaa acctcgctga
     2341 gcccgcccac tccggatacc atgtaacccg ggagatctca tcgagcatct tatcccgaag
     2401 atcggatatc cggaggaacc actggctggt ggcgatgaat atgatggggg tcttgcagcg
     2461 ccagcagtgg ccatagcggt gggcgagcct cccctgagcc aggagcttat ccctctcttc
     2521 gagatctgcg atgacgttgc tgtccgcctc cttgacatac tggcccaggt atttctctcc
     2581 cgcctcggca gtgtaccgcc cgtccccgcc taccgggcag aatatctcca ggtggtggtc
     2641 cagcccgagc tcataatcct caaggccgtg tccgggagcg atgtgaacgc atcctgtgtt
     2701 ctcagcggtg acgaagtctg cggcgtaaac gccatgctct atctctctct gccggggcac
     2761 cagatcgagc agaggatgct cgtacttcag tttctgcata tccttggctg aaaggccctg
     2821 caaaagctcg tagtcctggt agcggcccgt cctgaggact gaatcgacca attctgaggc
     2881 catgatcagg atctcggatt tgccatcctt ccaggccctg acccgagagt actggaagga
     2941 gggattgact gccaccgcca cattggcggg aatggtccag ggggtggtgg tccagatcac
     3001 tatgtaggtg ttctcctccc cgatcaccgg gaacttgaca tagatcgagt agtcggtctc
     3061 atcccagtat tccacctctg agtcggcgat ggccgtctgg cagcgggggc accagttgac
     3121 cacccttgag cccctttcaa gaaggttatg ctcgtgcgcc ttcttcagcg tccaccaggc
     3181 agcctccaga tactcgttct tcagcgtcat gtaggggtcg tcccagtcca tccaggcccc
     3241 tagaatcttg aactgggccg tcatgtcatc cttctggcgg agggcgaact ccttgcatct
     3301 ctggatgaac ttgtccaccc cataagcctc gatatccttt ttgttgcgga agccgaagga
     3361 ctcctctacc ttaacctcaa tgggcaggcc gtgcatatcc cagcccgccc ggtccttgac
     3421 ctcaaagccg ttcatagacc ggtagcggag cacggcgtcc ttgatcacct tgttccaggc
     3481 ggttcccaga tgaatgcggc ctgtggtata gggcggccca tccacaaaga agaacttctt
     3541 gccgcccaat ctcaattccc gaactttgcg gtagctatcc gctcgctccc acagcgatct
     3601 gacttcattc tcaagatctt gcgcattata ctgtccagcc acctcagaga tcacttgcaa
     3661 tcctccgaaa aacgatttgc atgcatgtag cacctctata tttagccttc gttgtcccat
     3721 tagagagagg agatttaggg aggaatattg atatacagat agccaaaacc aataattgag
     3781 gggttttata tcaaagagga gatctggata gagaagtacc ggccggagag gctggacgac
     3841 attgtgggcc aggacgagat cgtccgccgt ctgaagtcct atgtgaagac gcgaaacctg
     3901 ccccatcttc tattctccgg gccgccgggg gtgggcaaga ccgctgcctc catctccata
     3961 gtcaaagaga tcttcggcga gacctggagg aacaacttca tcgaattgaa tgccagcgat
     4021 gagcggggca tagatatcat ccgccataag gtcaaggact tcgcccggat ggcgcccctg
     4081 ggagaggcgg atttcaaggt tatatttctg gatgaggccg atgccctgac caacgatgcc
     4141 cagagcgctc tgcgtcggac tatggagaga tactccgcta ctaccaggtt catactctcc
     4201 tgcaactact cctccaagat catcgagccc attcagtccc gctgtgcagt ttaccgcttc
     4261 aagcccctct ctactgaagc ggtgaccaag aggatcaagt tcattgccag tgaggaggga
     4321 ctgcgggttt cggatggagg tctgtccgct atagagtatg tcgccggcgg cgacatgaga
     4381 aaggcaatca acgccctgca ggcggcagct ctcttgggcg acgaggtgga cgaggagacg
     4441 atttatcaga tcacatccac cgccaaaccg gaggagatca agagcttcat caagaccgca
     4501 atatctggag attttgtggg agcgcgcgcg atactggacg accttttact ctccaaaggg
     4561 ctatccggcc aggatgtggt gatccagatc caccgggcta tgctggacct ggatgatatc
     4621 gcagacaggg atcgggtaaa gctcatcgac cgtatcggag agatcgattt tcgcatgact
     4681 gaaggagcaa acgagagaat tcagctcgag gctctcttag cgtacttcgc cctgatggct
     4741 tcagagcaga gatgagcagc tgaattataa ttctgtagca tattgaacca gtcagggggc
     4801 tcagccattg aaaaccggca ttctgctatg cataagagcc gctttcagct cctcgattct
     4861 caccggtttg gatatgtagt cattcatccc cgcatcgaga cactcctctc gatccccttt
     4921 catggcgtga gcggtcatgg cgatgatgta gggctgatgg catgcggtga tgcgtatgct
     4981 cctggtggcc tccaggccat ccatctccgg catctgcaca tccatcagca ccacatcata
     5041 aggcctgctc tcaagtgctg ccagaacctc ccggccatca gcagcgatat cagccgtata
     5101 tcccagccgc tccagcattc ttatagcgac cttctgattg accaaattgt cctcggcaag
     5161 cagtattcgc aggccttttt ggatctcttg atcggacaga tcgagggtca ggtcaggccg
     5221 atccccatgg agcttcggat aaggctcgat ggaggcctcg gcctggatgg tgaaatagaa
     5281 gcttgagccc tctccaggaa cgctctccgc ccagattcca cctcccatca gctcagccag
     5341 atgcttgcag atggcaagtc ccagtcccgt gcctccatac ttgcgggtgg tggaggcgtc
     5401 tacctggctg aaggactgga acagccggct cattcggtcc tgggatatgc cgatccctga
     5461 gtccgtcacc cggaactgga tctcatggcc gtcctcgact ggaagagcgg atacatccaa
     5521 ccggacgaaa cccttatcgg tgaacttaac ggcatttccc aggagattga ccagtatctg
     5581 tctgagcctg gtcatgtcgc caatcagata tctgggaacc tgcggctcga cctcatagct
     5641 aacggagagg cccttgcgag aagcgtcctc tgccaccatg ttcaatgagg tctccaggca
     5701 ttcaatgagg tcgatgcgct ccttctccag ctccatcatc cccccctcga tcttggagaa
     5761 gtccaggata tcgctaataa cctttagcag agagtcacca ctgctgcgga tggtctcaat
     5821 gtagtccctc tgcacagagt cgatgttggt attgagaagc aggctggtga agcctatcac
     5881 cgcattcata ggggtccgga tctcatggct catattggcc aggaactctg acttggcctt
     5941 cgtcgccgat tccgccctat cttttgcctc gcgcaagtcc tgctccacct tctttcgctc
     6001 agtgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtgat atcctggccc
     6121 tgagcaatgg tggcaatgac acgcctcccg gtctcatcat atatattggc cgagttccaa
     6181 aggacggttc tcacctctcc attctggtgg catatgggga tctccactgc attccagctt
     6241 tccccggaga gggtcctctc gatataagcg tacgactccg tccggctctc atcgggaaag
     6301 aggattccca gcgatcggcc caagacctca tccgcccgcc agcctgtcag cctctcgaag
     6361 gcatgattaa acctggtgat gcaaaagttt gggtcccaga cgatgatggg ggcattggca
     6421 tggctgagga gattatccag atagtccctg gtctcccgca ggtcggctgc cacccgcttg
     6481 aactcctcgg aagaacgctt tttttcagtt atgtcgttgc caatgcataa aatctctgca
     6541 acctctcccc gctcgttttg gaccagccgg tgggtccagg caatccagac cggctctcca
     6601 ctggacttca tgttccccgc tatatgagag gagcattctt ctggatgctc ccttatatgg
     6661 cggattatcc catcgagatt gcagcccttg cccccaggtg gaaagatggt ctcggaaaca
     6721 tttctcccca ggatatcatc ctcattgtag ccgaagaaat gcaggccgaa cttgttgatg
     6781 aacctgatag ctccactggt atccacccgg atgatgacgc tgcttgaggt ctccaccagc
     6841 tccctgtact tctgctcgct ctccctgagg gcggattccg ctatgaatcg gccgatgaac
     6901 ccccctatgg cgctggccat ggtcatcagt atgaaggccg cgctccagct ccaatccctc
     6961 tccagcctat ggtcctcgaa accgatgaag ccccagaatc ggtcggtggt gaatatgggg
     7021 acgatcatga atgatcgcac ccccatcatc ttcagcggcc ccctcgccgg cgggggcagg
     7081 tcacgggttc ttccccggat gggcattcct ccggagagga tctggaacca ttggggaatg
     7141 gagctgtatg ccttgcagct cagatctgga tggcggatct tggccgagcc tgcatctcca
     7201 tcattgccag atatccattc atacccaagc gagatctgat ctcttccctc ctcgtccaga
     7261 taatgctcaa atatgcaggc ccggtccaca ttggccgagc agcccagtat ctccagggac
     7321 agattaaggg caacatccat ctcccgagag atcaggagct ggtttgtggc cagggcagcc
     7381 cctgccatga tcctatctct ctcgcgcaga tcgtcatttc ggccctgatg ctctatccaa
     7441 aaaattgcat tttcagaggg aggactatca tcatactcct gcccagaaga gggatgcgct
     7501 tctctaaatg tgattatgga tctgccttca tagcccttcc cctgcatagt agcaatggag
     7561 tactcaattc tccttctctg gagatcgaca tatcctcgat ggatcgggcc atcagcgctc
     7621 ttcgcggtct ttaggatgcc tgagcacagg tccttcaaga ccccgccctt gccacaattc
     7681 agctcttcca gagatcttgc catcctctct gcagcagggt tcgaatagag gatctccccg
     7741 gatggatcgc agatgcatat cccatcctcc agtatctcca gggtggactg aaacatcctc
     7801 tcaggattca tgagatcgcc ccaccaggac cgcccggaca ggagcggcct ctgcatcttt
     7861 tatcttcagg ggaaggcaga tcagccagta tctgccaggg gaaacggcac taagatcgat
     7921 cccctccagg atcagaacat cgtttttcag caggatattg tgaacgggga gggactcatc
     7981 ctcatatcga tccactgaga tatagtcgat tcccaccagg gcggctccca gactaacgca
     8041 taactctgct gcagggggag agagggagac atattcatcc cggaatgtgg ggttatgcat
     8101 cagcccctgg aaggaattgc tggtcttaaa aaggatggcc tcacctctct ggatattgac
     8161 atcatgcagc gcctgggcgg ggacggcatc gcaattccgg acgcagatca cccgggcagg
     8221 agtgatgaac ctttccggcg gatagctgtc cagagggaag ccatccctca gtatatgaga
     8281 ggggaagtca atatgagttc ccgtatggga gcccaaagag agaacagaga ggctatagtt
     8341 ggatcccttc ccaagtcccg ccatccactg cctggagaag ggactgtctc c
//
LOCUS       DAOSGP010000029         8247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_7425_length_8247_cov_16.159302, whole genome shotgun sequence.
ACCESSION   DAOSGP010000029 DAOSGP010000000
VERSION     DAOSGP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7425_length_8247_cov_16.159302"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1657)
                     /locus_tag="PLV49_01700"
     CDS             complement(89..1657)
                     /locus_tag="PLV49_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOG97584.1"
                     /translation="MKFRVFLRLLAQLLKLLAILLLVPGAVAAVYAETGGVIAFAATS
                     LLSLATGIALGRLSSEEEPGLREAFALVALGWVAVAFFGALPYVFLGMSLIDGLFESM
                     SAFTTTGSSVFTEINHLGYWKMNQTLADSSLVAEMQMRLVRMTGNSILLRIRSGPDDT
                     IMGLLFWRSFAQWLGGMGIILLFVAILPRLGVAGRQLYRAEVPGPDKESIKPRIKQTA
                     RMLWMVYILLTLVEVALLLLAGMPPFDSFCHAFSSMATGGFSPRGLSIMAYNSWIIDA
                     IITLFCFLAGANFALHYRMITSDRKALISDTEFRAYALIILLATSVIILWGGLEGDIY
                     QKIQLAGFQVVSILTTTGFATADFNQWTATARITLLLLMIIGACAGSTAGAIKVVRLI
                     LIFKSAYRELIRTLHPKAVQSVRLGDMVVKEEILRASNIFVSMYIIIFAIASLLLAMI
                     SYSDPKMDMESVLSAVATTLGNVGPGFGVVGPMFSFADIHPAGKMLLILCMWIGRLEV
                     VTAMVLFLPEFWKK"
     gene            complement(1741..3054)
                     /gene="trkA"
                     /locus_tag="PLV49_01705"
     CDS             complement(1741..3054)
                     /gene="trkA"
                     /locus_tag="PLV49_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOG97585.1"
                     /translation="MRILITGAGEVGFLIASELYADHDVTVIDKDPDACARLQDMDVK
                     VMQGNAANARLLSDADIKKADIVVAATGNDEVNVITCIIASHLGVRQTIARVSNPEYI
                     DQPVQDRKEIGISYMICPELTMAEDMASSLYFSSMLMNRDLANGLVELVEFKVTPEMK
                     IQGPVENIVLPNNCNLIAINRAGRIAIPKEGDEIRNDDHLMLLCDARSLPDLGSMLHE
                     SKTTQKAMIVGGGMVGFYLASRLEKKGIDVKLIEKDADRCAEIADKLSGTMILNGDGS
                     DLALLQEEGAGAMDVVYAVTGLDDKNLLCSLLVKQLGATKILSRVNRNVYIKLFEMVG
                     VDRAVSPGQVTADAVLQRVIGGEEVITLSDERMELVDFIAKPGAKIVGKSISKELPKN
                     SLAGMVIRDGAPIVPYEDFKVSAGDRVFVMSMPDAVSKVKKLFAA"
     gene            3211..4413
                     /gene="larC"
                     /locus_tag="PLV49_01710"
     CDS             3211..4413
                     /gene="larC"
                     /locus_tag="PLV49_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOG97586.1"
                     /translation="MFALLFDPSAGAAGDMIMASLLDLGADEKRVRKAVESVGCTLEV
                     SRQEKGHISATRAQVISDRRYHSLEEAVSILKSSSLQEKALKKALAALDILAEAESRV
                     HDVPKSRAHFHEVGALDALADIAGSCEAASSLKADRILSRPVSVGGGYVQSAHGLLPV
                     PGPAALEILRAHNIPWIGGPVDDELLTPTGAALLAALVDEFVPRFPLIQAVRVGYGAG
                     RKDLATPNLLRTLEAKVISPTRGAGMDHQKDRVVQLETNVDDVTGEVLGHLIEQLMQA
                     GALDVSVIPAVMKKGRSGNVIRAISSHEDMSKLAGMIVRETGSLGVRVFPSLHRFVAE
                     REETTADLNLEGVTYSVAIKISRMDGSLVNVKAEYEDCRRIAKEAGVPLRMVIKKAEE
                     LGWRAADK"
     gene            4616..5887
                     /gene="hemL"
                     /locus_tag="PLV49_01715"
     CDS             4616..5887
                     /gene="hemL"
                     /locus_tag="PLV49_01715"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOG97587.1"
                     /translation="MMHFDSSIRLFDQAKRLMPGGVSSPVRAISPYPFYTARAIGPYL
                     WDCDGNRFIDYCLAYGPMILGHRHPAIKEAVSDQMDRGWLYGTPSELEVRLAERVSSL
                     YPSMERLRFVSSGTEATMAAIRVARGSTGRDMIIKIEGGFHGAHDSVLVKAGSGATTL
                     GIPDSLGVPADATKNTIQVPYNDPAALEEVLERFSGRIACLIMEPVLGNIGPILPDKG
                     YLQTARRLTEKNDVLLIFDEVITGFRLSLGGAQELFGIKPDLTTLGKIIGGGFPIGVF
                     GGRADLMDQVAPGGAIYQAGTYNGSPASLAAGLATLDVMEKEDTIKKLNRSGESMRLA
                     LKDMVEDLGQDWSVVGIASMFKVFFGPEPHNYAQALMCDRQGYFEFFRRMLDSGIFLT
                     PSQYETDFISAAHNQDVIETTLEAFRSCLKS"
     gene            5917..>6481
                     /gene="hemC"
                     /locus_tag="PLV49_01720"
     CDS             5917..>6481
                     /gene="hemC"
                     /locus_tag="PLV49_01720"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOG97588.1"
                     /translation="MRQTQIVQERLKGLGIQTQLCKVKTSGDVFLDKPLHQLTGFGVF
                     VAEIDERMLSGKIDLAVHSMKDLPTKRPKELIVSAVLKRDSPYDVLLCREKSVKSIEE
                     LDQGARVGTSSMRRMAQLLRARPDLNILSLRGNLQTRLGKLERGDYDAIVLAEAGLER
                     MGYHPDYVRLDGERFVPSANQGTIIVVA"
     assembly_gap    6482..6581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6582..6962
                     /locus_tag="PLV49_01725"
     CDS             <6582..6962
                     /locus_tag="PLV49_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOG97589.1"
                     /translation="DGERFVPSANQGTIIVVAKAGSEAEACSRALDDAHARSETMIER
                     KIMETVGGGCVVPMAVHALVQKGEARVLAEVLSPDGKRFVRLDERISLVQNHLAVAED
                     MGERLMRMGGRELVDEAVRFFGNR"
     gene            6949..>8247
                     /gene="cobA"
                     /locus_tag="PLV49_01730"
     CDS             6949..>8247
                     /gene="cobA"
                     /locus_tag="PLV49_01730"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOG97590.1"
                     /translation="MAIGKVYLVGAGPGDPELLTIKGRKRLMEADVVLFDRLLDSRML
                     DGVKGELIDVGKNAGRHKLKQEEINQLLIEKAKEGKIVVRLKGGDPYLFGRGGEEAIA
                     CRDWGIPFEVVPGVTSAIAAPELAGIPVTHRKVASALTIVTGHEEPGKDSPLDWRAMA
                     GLDGTLVVLMGVSRLEENTNRLIEAGKSPQTPAAMVEKGGWPDQRLISGTLGDIAERA
                     KEENVQSPAILVVGDVVRLAEVLAPERIAILRPAGQQEESVELAERYGFIPLSAPAIA
                     LEKNPLPEDLQERIAAAECVAFTSANGVHIALGNKAISGALAEKKIVSIGPKTKQALN
                     EYNIQSEMPESYSSEGLERMLKGRYKSILFLRSAQGSQYLSDGLREAGIVVDDIPLYG
                     VVNSSDPRLDRLIERAGEVDIFAFTSSSTARNLLERARAMG"
BASE COUNT         1800 a         2280 c         2406 g         1661 t
ORIGIN      
        1 ccggtggctg catctcccag gcctggtcgg tgggagaggt tctcagggct tatgaagagc
       61 tgcgagatgg atagaaaaaa aattccattc attttttcca gaactctggc aagaagagga
      121 ccatagcggt caccacctcc agcctgccga tccacatgca taatatgagg agcatctttc
      181 ctgcaggatg gatatccgca aagctgaaca tcggccccac cacaccaaat cccggtccga
      241 cattccccag ggtcgtcgcc actgccgata ggacgctctc catatccatc ttcggatcgc
      301 tgtaggagat catggccagg agtagggagg ctatggcaaa gatgatgatg tacatggaga
      361 cgaagatatt gctcgccctt aaaatctcct ctttcaccac catatcgccc aaccggacgc
      421 tctgaaccgc cttgggatga agagtgcgaa tcagctctct gtatgcgctc ttgaatatca
      481 gtatcaatcg aacaaccttg attgctcctg cggtggatcc ggcacaggcc ccgatgatca
      541 taagcaagag gagggtgatc ctggcggtgg cggtccactg attgaaatcg gcagtggcaa
      601 acccggtagt ggtcaggatc gagaccacct ggaagccggc cagttgaatc ttctggtaga
      661 tgtctccctc cagccctccc cagaggatga tgaccgacgt ggccaaaagg atgatcaagg
      721 cataagcccg gaactccgtg tcgctgatca gggccttccg gtcggaggtg atcatcctgt
      781 agtgcagggc gaagttggcc ccggccagga aacaaaacag ggtgatgatg gcatcaatga
      841 tccagctatt gtaggccatg atgctcaatc cccggggcga gaatcctccc gtggccatgg
      901 atgagaaggc atggcagaac gagtcaaagg ggggcattcc tgccagaagg aggagggcga
      961 cctccaccag ggtcaggagg atatagacca tccagagcat gcgagcggtc tgcttgatcc
     1021 tgggcttgat ggattcctta tccgggccgg gaacctccgc ccggtacagc tgccgtcccg
     1081 ccactcccag gcggggcagt atggccacga acagcagtat tatccccatc cctcccagcc
     1141 actgggcaaa tgacctccag aagagcagcc ccatgattgt atcatctggg ccgctcctaa
     1201 tccggaggag gatggagttg ccggtcatcc tcaccagccg catctgcatc tccgctacca
     1261 ggctgctatc ggccagggtc tggttcatct tccagtatcc caggtgattt atctctgtga
     1321 atacagacga tccagtggtg gtgaaggcgg acatggactc gaagaggcca tcgatcaggc
     1381 tcatccccag gaagacataa ggaagcgctc cgaagaaggc gacggcaacc cagcccagag
     1441 ccaccagggc aaaagcctct ctcaatcccg gctcctcctc agatgacagc cttcccaggg
     1501 ctatccccgt ggccaggctt aatagagagg tggcagcaaa ggcgatcacc ccacccgtct
     1561 cagcataaac agcagctacc gcacccggaa cgagcagcaa gatggccagc agcttcagca
     1621 gctgtgcaag cagtcgcaga aacaccctga atttcatcaa acaatgccgc accaaaatgg
     1681 cggagggcat cctcctgaaa gcgccactta tctcctgttc ctataaaaag gatctgctca
     1741 ttatgccgcg aatagcttct tgaccttgga gacagcgtcc ggcatggaca tcacgaacac
     1801 ccgatcaccg gctgacacct tgaagtcctc ataggggaca ataggcgcgc cgtctcttat
     1861 caccatgccg gcaagggagt tcttgggaag ctctttggat atcgacttgc ccacgatctt
     1921 cgctcccggc ttggcgatga aatccaccag ctccatcctc tcatcgctca gtgtgatgac
     1981 ctcttctccg ccgatcaccc tttggaggac ggcatcggca gtcacctgcc ccgggctgac
     2041 cgctctgtcc acccctacca tctcgaagag cttgatgtag acgttcctgt tgacccggga
     2101 gaggatcttc gtggcaccca gctgctttac caagagggag cataacaggt tcttgtcatc
     2161 aaggccggtc acagcataga ccacatccat cgcgcccgcc ccctcctctt gaaggagggc
     2221 gagatctgat ccatcgccat tgaggatcat ggttcccgag agcttgtccg caatctcagc
     2281 gcaacgatcg gcatccttct cgatgagctt gacgtcaatt cccttcttct ccagcctgct
     2341 ggccaggtag aagcccacca tccctccgcc cacgatcatc gccttctgag tggtcttgga
     2401 ttcatgcagc atacttccca ggtcgggaag ggacctggca tcgcacagca gcattaggtg
     2461 gtcgtcattt cttatctcgt ctccctcttt cggtatggct atccgacccg cccggtttat
     2521 ggcgatcagg ttgcagttat tagggaggac gatattctcc accggcccct ggatcttcat
     2581 ctctggtgtc accttgaact ccaccagctc caccaggccg ttggccaggt cccggttcat
     2641 gagcatggat gagaaataaa gggagcttgc catatcctcg gccatggtca gttccgggca
     2701 gatcatgtat gatatgccga tctcctttcg atcctggacc ggctgatcga tgtactcggg
     2761 attgctcacc ctggcgatgg tctgcctgac ccccagatga gaggctatga tgcaggttat
     2821 cacattcacc tcatcgttcc cggttgcagc cacgactatg tccgccttct tgatatccgc
     2881 atcggagaga agccgggcgt tggcggcatt cccctgcatg accttcacat ccatatcctg
     2941 aagcctggca caggcatcgg gatccttatc tatgacggtg acatcatggt cagcataaag
     3001 ctcgctggcg atgagaaagc ccacctcgcc agctccggtg atcaggatac gcatcttgtt
     3061 tcctccacat caaccagggc gcaaaggaga tccaaggata agaattgatc catccctgtt
     3121 ataagcgttt tccattcgtt tgagcacttc cattcgtccg tcctctgaaa ggccgatcca
     3181 tatatatcgg cagagagaca gctatcatcc atgttcgccc ttctcttcga ccccagcgcc
     3241 ggcgcagccg gagacatgat catggcctcc ctcttggacc tgggagcgga tgaaaagagg
     3301 gttcggaagg cagtggagtc ggtgggctgc accctggagg tctcccggca ggagaagggg
     3361 catatctctg ccaccagggc ccaggtgatc tcggaccgga ggtatcattc cctggaggag
     3421 gcggtatcca ttctgaagag ctcaagcctc caggaaaagg cactgaagaa agcccttgcc
     3481 gccctggata ttctggcaga ggcggagagc cgggtccatg atgtgcccaa gagccgggcc
     3541 cattttcatg aggtcggtgc cctggatgct ctcgctgata tagcaggttc ctgtgaggct
     3601 gccagcagcc tgaaagcgga tcgaatcctc tcccggccgg tctccgtcgg tgggggatat
     3661 gtccagtcgg cccatgggct ccttcccgtt cccgggccgg cggccctgga gatcctgcgg
     3721 gctcataata tcccctggat aggggggccg gtggacgatg agctcctcac tcccactggg
     3781 gcggccctac tggcggcttt ggtggacgag ttcgtgcccc gctttcccct catccaggcg
     3841 gtgagggtgg gctacggcgc aggcaggaag gacctggcca cccccaacct cctgcgaacg
     3901 ctggaagcca aggtcatatc tccaaccaga ggggctggga tggatcacca aaaggatcgt
     3961 gtggtgcagc tggagaccaa tgtggacgat gtcacaggcg aggttttggg ccacctgata
     4021 gagcagctca tgcaggcggg agcgctggac gtctcagtca ttcccgcggt gatgaagaaa
     4081 gggagaagcg ggaacgtcat ccgggcgata agcagtcacg aggatatgtc gaagctggcg
     4141 ggaatgatag tcagggagac gggatcttta ggggtcaggg tctttcccag cctgcaccgt
     4201 tttgtggcgg agagagaaga gacaacagcg gacctgaatc tcgagggggt gacctatagc
     4261 gttgccataa agatcagccg gatggacggc agcctcgtca atgtcaaagc ggagtacgaa
     4321 gactgcagaa ggattgcaaa agaggcaggc gttcccttgc gcatggtcat taaaaaagcg
     4381 gaggaattgg gttggcgggc agcagataaa tgaatcgtgc catccctttg atttccattt
     4441 gatctatgcg gttttttata actatgctgc ccgcgtgtcc tcttcaccgc tctctattta
     4501 ttctaataac cttttcatta tactaatata gtcttggtag taattcctaa tatatatagt
     4561 ttttgtaatt aatagtgtcg taagattgat taatctgccc gaagagaaag ctcccatgat
     4621 gcattttgac tcttccatca ggctctttga ccaggctaaa agactgatgc ccggcggggt
     4681 ctcaagcccc gtgcgcgcca tatctcctta tcccttctac accgctcggg caataggccc
     4741 ctatctatgg gactgcgacg gcaacaggtt catcgattac tgcctggcct acggcccaat
     4801 gatcctgggc caccgccatc ctgccataaa agaagcggtt tcggatcaga tggaccgggg
     4861 ctggctttat ggtaccccaa gcgagctgga ggtccggctt gcggaaaggg tgtcctccct
     4921 ctaccccagc atggagcggc tgcgctttgt gagcagcgga accgaggcca caatggctgc
     4981 cattcgagtg gcccggggca gcaccggcag ggatatgatc atcaagatcg agggcggatt
     5041 ccatggcgct catgatagcg tcctggtcaa agcgggttct ggtgccacta ccctgggcat
     5101 tcccgactcg ttaggcgttc ccgcagatgc caccaaaaac accattcagg tgccctacaa
     5161 cgacccagct gccctggagg aggtcctgga gcgtttctcc ggccggatcg cctgcctgat
     5221 catggagccg gtcctgggaa atatcggccc catcctgccg gataagggct acctgcagac
     5281 ggcgcgccgc ctgacggaga agaacgatgt cctcctgatc ttcgacgagg ttataaccgg
     5341 attccggctc tccctgggcg gagcgcagga gctcttcggc atcaagcccg atctgaccac
     5401 cctgggaaag ataataggcg gaggcttccc cattggggtc ttcggaggcc gggcagatct
     5461 gatggaccag gtggccccag ggggagcgat ctatcaggcg gggacgtaca atggaagtcc
     5521 ggcctctctg gctgctggcc tggcaacctt ggatgtgatg gaaaaggagg ataccataaa
     5581 aaaactcaac cggtctggag agagcatgag actggccctt aaggatatgg tggaggatct
     5641 tgggcaggac tggagcgtgg tcggcatcgc ctccatgttc aaggtattct tcggcccgga
     5701 gcctcacaac tatgcccagg ccttgatgtg tgatcggcag ggctacttcg agttcttccg
     5761 ccggatgctg gattctggca tcttcctcac ccccagccag tatgagaccg atttcatttc
     5821 tgcagcgcat aaccaagatg tgatcgagac caccctggag gcgttccgct cctgcctgaa
     5881 aagctgatcg tggggacgag gggcagccct ctggcattgc gccagacgca gatcgtgcag
     5941 gagaggctga agggccttgg catccagacc cagctgtgca aggttaagac cagcggggac
     6001 gtcttcctgg acaagcccct ccatcagctc accggttttg gggtctttgt ggcggagata
     6061 gacgagcgca tgctttccgg caagatcgat ctcgctgtgc acagcatgaa ggacctcccc
     6121 actaaaaggc caaaggagct gatagtctcc gccgtcctga aacgcgactc gccatatgac
     6181 gtcctcttat gcagggagaa atctgttaaa agcatcgagg agctggacca gggggcacga
     6241 gtgggcacat cgagcatgcg gcggatggcc cagcttctcc gcgcccgccc cgacctgaac
     6301 attcttagcc tgcggggcaa tcttcagacc aggctgggca agctggagcg gggagactac
     6361 gatgccatag ttctggccga ggccggcctc gagcgcatgg gctaccatcc agattacgtg
     6421 agacttgatg gggagaggtt cgttccctct gccaaccagg ggacgataat cgtagttgca
     6481 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatggggag aggttcgttc
     6601 cctctgccaa ccaggggacg ataatcgtag ttgcaaaggc cggatctgag gctgaggcct
     6661 gctcccgcgc tctggatgat gctcatgcta gatctgagac catgatcgaa cgcaagatca
     6721 tggagaccgt gggcggaggc tgcgtggtgc ccatggcagt ccatgcgctg gtgcaaaagg
     6781 gagaggcgcg ggttctggct gaggtcttat ccccggacgg caagaggttc gtccgcctgg
     6841 acgagaggat ctctctcgtc cagaatcatc tggcagtggc agaggatatg ggagagcggc
     6901 ttatgaggat gggaggaaga gagctggtgg acgaggcggt gaggtttttt ggcaatcggt
     6961 aaggtttatc tggtgggagc aggtcccgga gacccggagc tgctgacgat taagggaaga
     7021 aagaggctca tggaggcgga tgtagtccta ttcgaccggc tgctcgactc caggatgctg
     7081 gacggggtaa agggggagct tatcgatgtg ggcaagaacg cgggaaggca taagctcaag
     7141 caagaggaga taaaccagct cctgatagag aaagccaaag agggcaaaat cgttgtccgg
     7201 ctgaagggcg gagaccccta cctcttcggc cggggaggcg aggaggcgat cgcctgcagg
     7261 gattggggaa tcccgtttga ggttgtcccc ggcgtcacct cggccatcgc cgccccggag
     7321 ctggcgggaa ttccagttac ccacagaaag gtggcctcgg ccctgaccat agtcaccggc
     7381 catgaggagc cgggaaagga ctcgcccctc gactggcggg ccatggccgg gcttgatgga
     7441 accctggttg ttctcatggg cgtctcccgg ctggaagaga acaccaatag gctcatagaa
     7501 gcgggaaagt ccccccagac cccggctgcc atggtggaga agggcggctg gccggatcag
     7561 aggctgatct cggggaccct gggggatatc gccgagaggg caaaggaaga gaatgtccag
     7621 tctccggcca tactggtagt gggagacgta gtccggctgg cagaggtcct cgccccggag
     7681 aggatcgcca tcctcaggcc ggcgggccag caggaggagt cggttgaact ggcggagcgc
     7741 tacggattta ttcccctgtc cgctccggcc atcgccctgg agaaaaatcc cctgcccgaa
     7801 gatctgcaag agaggatcgc tgccgctgaa tgcgtggcct tcaccagcgc caatggtgtg
     7861 catatcgctc ttgggaataa ggccatatcc ggagcattag ctgaaaagaa gatcgtctcc
     7921 atcggtccca agacgaagca ggccctgaac gaatacaata tccagtcgga gatgcctgaa
     7981 agctacagct ctgagggcct ggagaggatg ctcaagggaa gatataagag cattctcttc
     8041 ctgcgcagcg cccaggggag ccagtatctc tccgatggcc tgagggaggc gggaatcgtg
     8101 gtggatgaca ttccccttta tggagtggtg aattcatccg atccccgcct ggaccggctg
     8161 atagagcggg caggcgaggt ggacatcttc gccttcacca gctcctcaac agctcgaaat
     8221 ctgctggaaa gagcaagagc gatggga
//
LOCUS       DAOSGP010000030         8105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_7612_length_8105_cov_16.845466, whole genome shotgun sequence.
ACCESSION   DAOSGP010000030 DAOSGP010000000
VERSION     DAOSGP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7612_length_8105_cov_16.845466"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..728
                     /locus_tag="PLV49_01735"
     CDS             153..728
                     /locus_tag="PLV49_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HOG97591.1"
                     /translation="MSSLRLIDNTANPAPLGLLGFGMTTVLLNIHNAGYYELNTMILA
                     MGIFYGGMAQIIAGIMEWKKNNTFGTTAFTSYGLFWLTLVGLLLMPSLGLGTATSKVA
                     FAAYFFMWGLFTFVMFIGTLRINRALQVVFLTLTILFFLLAARDYFGSAAIGTLAGYE
                     GIICGLSAIYAALAQVLNEVYKKVVLPLGPV"
     gene            complement(838..1725)
                     /locus_tag="PLV49_01740"
     CDS             complement(838..1725)
                     /locus_tag="PLV49_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97592.1"
                     /translation="MKGVLVAIAIVVAQLLCCIAMANPPLVPPVQYEQFCESQKVSGT
                     GIIDVSTSIVDKKIALEYYNTMAGDGDFELDSEHAYSQNSDKLKRNISSVNGGNESGL
                     NMYENTKLTYAGDTPLMGAKFLNSKAFYGGIGANVQETFAVTEMEKDQTTFFVSTTPY
                     DPQEPADPQCDDYLKLLQTYYPLEYSKYMAESKHSITPEELIKRLKNAGRDTDAVEKL
                     MTSKDGTYNPAHLIGLETKNSFNGTWGTDATWHRIFYKDIKAHELFSGTFEAEKLIKF
                     HENPVPEKDNQPPCDGIDC"
     assembly_gap    1797..1896
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1897..>3488
                     /locus_tag="PLV49_01745"
     CDS             <1897..>3488
                     /locus_tag="PLV49_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG97593.1"
                     /translation="EDKPIFLSVGYSTCHWCHVMAHESFEDPNVARLLNQSFICIKVD
                     REERPDIDQIYMAAAIAVSGRGGWPLTVMMTPDKKPFFAATYIPKKGHMGLTGLMELI
                     AQVKEIWDNDRESLLSSANIIVDHLKGRQSGRGADVQKEAHKDTLSGSPFDSSLLSRG
                     YSALSSIYDPENGGFGTAPKFPTPHHILFLLRCWKRTKNILPLEMAKTTLQGMRMGGI
                     YDHVGFGFHRYSTDPEWFVPHFEKMLYDQALLAMAYAEAYQATGEEEYARTVREILEY
                     IRRDMTSPEGGFYSAEDADSEGEEGKFYTWTAVELKEALGEEDFRLLIRLFDVYESGN
                     YEGERNILRQRSSFSDAASVLKIPEEELYHRSSDMISRLYLARERRVHPLKDDKILTD
                     WNGLMIAALARAAGALQDPDLAMAASRAADFLLEVMRTPEGRLMHRYRQGADIQANLD
                     DYAFLIWGLIELYEATFDVKYLKAAVRLNEIMEQHFWDGEAGGFFFTADDGEELLVRK
                     KEYYDGALPSGNSIALLNLLRL"
     assembly_gap    3489..3588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3589..4030
                     /locus_tag="PLV49_01750"
     CDS             <3589..4030
                     /locus_tag="PLV49_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG97594.1"
                     /translation="DGALPSGNSIALLNLLRLLHLTGDTSLEGKAALLARSALPAVSA
                     QPLGYTMLLCALDYALGPTYEVALAGSLEEGGLKEMLAAIRSRFLPNKAVVLASGSEI
                     VMVAPFTRDLVPVKGKAAAYVCSDHVCQLPATSAAELMALLEKN"
     gene            4444..5061
                     /locus_tag="PLV49_01755"
     CDS             4444..5061
                     /locus_tag="PLV49_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK-like ATPgrasp N-terminal domain-containing
                     protein"
                     /protein_id="HOG97595.1"
                     /translation="MYTLVEDDSQYIVNESYFYKTEPYYAIVKSENEGIITTPSSRDV
                     LDAYIVPICLEKAKLAGISVCDWTVSYQFVPLPAIIYGLNYFSTPSEYFLVNDHDEAK
                     KVVKHITNHGRYPFCYQKIADTSAVTRCVSIFGRSLNCCEEVAEIAEKIYQVFKLPLV
                     EIVMVKDNSGTYNLSSLAPVKYSQLSKEEKEMLQDLLDKRVKRFE"
     gene            5054..6064
                     /locus_tag="PLV49_01760"
     CDS             5054..6064
                     /locus_tag="PLV49_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOG97596.1"
                     /translation="MSKLGIFVNRKTLSSSSQLMALVKCRDVAESMGHTVEFIFPVDM
                     KKIPRLDALFIRADTDPMNTTYVAARMAEMYGVPVIDDPTSIRICADKINMYMHLQKE
                     GVSIPKTRFLKKRELDDLQARQLFDELGSPLILKEPSTSFSVRVEKVSDVEELLKVAK
                     RFFKLSDWIVVQEYIESRFDWRVGVINGQLLYACRYIIPSETFKIQASVNGHIVYCDV
                     ESVPADQVPPEVIDLGKHAAGAIGKGLYGVDIKESNGKLFVIEVNDNPSLEGGEDEHY
                     PEMFGQIISHLVSAQALDDARDLSMETISSAGLMGRYPQEAESACIQVGQLGESDIYS
                     KR"
     assembly_gap    6154..6253
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6254..6703
                     /locus_tag="PLV49_01765"
     CDS             <6254..6703
                     /locus_tag="PLV49_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOG97597.1"
                     /translation="DCHLAGGCCFEARPPLSQKRIDMLMENGVSADAIEFVGYKRLRL
                     RQDGFCVLFRDGKCSIHDIKPETCVAGPFTFDIEGDMLQIFLKRESICPMVRFLRANR
                     KAYDALFETSVEKIMDLIRSVPEEEMAQILQIDEPQTDLVAEIRLED"
     gene            6710..7891
                     /locus_tag="PLV49_01770"
     CDS             6710..7891
                     /locus_tag="PLV49_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HOG97598.1"
                     /translation="MPLQFGRVSRAAMHRAQPAQSLLGTEHEYSINDSTFRPLAISDR
                     IIERINGRVEHEVAFGGIRVSKELQKHVLELIPASPGNLGRLEENLFSGLQELFRATK
                     NEYQFLGLGMHPLLTLDQTTYWDHDEQDYYQAYDRLFGIKQHGWLNIQALQINIPYRD
                     EQELVGMFNKIRSLMPYLVAVTASSPFVEGQVNSYMDSRLVYYRRNQSQIPQICRNVI
                     PERLEGIRDYVEINRNIYRELKKQHAQILCHEWVNSRGEIVRFTRRCLEIKAIDEQEC
                     LHSDMAICAFIMALLRTDLELEDDESHLLAMLEDGMRRGVAAMRPELERLYALAERNA
                     TREEQRYLPLIEKRIEQGSLAEIMVQRQKEEKEILPMLSQLAWCLKENIPYSAEPGRC
                     G"
BASE COUNT         1897 a         1903 c         2186 g         1819 t
ORIGIN      
        1 caaaaagata tctaagtggg gttgatatcg atcatttagc actattatca attaatattt
       61 ttaaggataa aggattaata tttaggaata taatgatggc tgcatcagta atctaggagg
      121 gaaagaatac ggaagatttt aagaaaggag cgatgagttc gctgcgacta atcgataaca
      181 ccgcaaatcc agctccatta ggcctgctgg gctttggtat gaccactgtt ctgctgaata
      241 ttcacaatgc aggatactat gagctgaata caatgatcct ggccatggga atattctatg
      301 gcggcatggc ccagatcatc gccggaataa tggagtggaa gaagaacaac accttcggga
      361 ccactgcctt cacatcctac ggcctcttct ggctcactct tgtgggattg ctcctcatgc
      421 caagtcttgg ccttggcact gcaaccagca aggttgcctt tgcggcctat ttctttatgt
      481 gggggctgtt cacctttgtg atgttcatcg gaaccctgag gatcaataga gctctgcagg
      541 tagtattctt aactctaacc attctattct tcctgctggc ggccagagac tacttcggaa
      601 gcgccgccat cgggacgctg gccggatatg aaggcatcat ctgcggactg tctgcgatct
      661 atgcagcact ggctcaggtc ttgaatgagg tatacaagaa ggtcgtcttg ccactgggac
      721 cagtataaaa aggcagcagc atttgcataa taaggatcgt gaccttccga tcctgtaatt
      781 ctttttttaa gcaattcctt ataaaaatcg caaaaagagg tgattgccag gagctaccta
      841 gcaatctatg ccatcacagg gtggctggtt gtccttttcg ggtacgggat tctcatggaa
      901 ctttatgagc ttctcagcct cgaatgttcc ggagaacagc tcgtgggctt tgatgtcctt
      961 gtagaatatc ctatgccatg tggcatccgt gccccaggta ccattgaagg agttcttggt
     1021 ctccaggccg atcaggtggg ccgggttata ggtaccatcc ttggaggtca tcagcttctc
     1081 gactgcatcg gtgtccctac cggcattctt cagccttttt atgagctcct caggagttat
     1141 gctatgcttg gattcagcca tgtacttgga gtactccaga ggataatagg tctgaaggag
     1201 cttcaagtag tcatcgcact gaggatcagc gggctcctgg ggatcgtatg gcgtggttga
     1261 gacaaagaat gtcgtctggt ccttctccat ctctgttacc gcgaaggtct cctggacgtt
     1321 ggctcctatg ccgccataga acgcctttga attgaggaac ttggctccca tcagtggcgt
     1381 gtctcccgca taggtcaatt tcgtattctc atacatgttc aggccggatt cgtttccacc
     1441 attaaccgag gatatatttc tcttcagctt atcggaattt tgcgaataag catgttcgga
     1501 gtcgagctcg aaatcgccat ctccagccat tgtattgtag tattcgagag cgatcttctt
     1561 atcgactatg gatgtgctaa catctatgat tccggttcct gatacctttt ggctctcaca
     1621 aaactgttca tactgtacag gaggcacaag cggtggatta gccatcgcta tgcagcaaag
     1681 caattgggct acgactatag ctatcgcaac taatactccc ttcatatttc aaattcaccc
     1741 ctatactcca aagagtatac tcccaattta tctcaaaagg atataaacat atcgaannnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggga ggataaaccg atctttctct
     1921 ctgtgggcta ctccacctgc cactggtgtc atgtcatggc ccacgagtcc tttgaggatc
     1981 ccaatgtggc ccgcctcctg aatcagtcct tcatctgcat aaaagtggat cgggaggaga
     2041 ggccggacat agaccagatt tacatggccg cggccatagc tgtcagcggc agagggggat
     2101 ggccattgac cgttatgatg actcctgata aaaaaccctt ctttgctgcc acttacatac
     2161 caaaaaaagg gcatatgggc ctgacgggat tgatggagct gatcgctcag gtcaaggaga
     2221 tatgggataa cgatcgagag agcctcttga gctcggcgaa catcatcgtg gaccatctga
     2281 agggacggca gagcggcagg ggagcagacg tgcagaagga ggctcataag gacaccctct
     2341 cggggtcgcc attcgattct tctcttctct cccggggcta cagcgccctc tcttcgatct
     2401 acgatcctga gaatggtggc tttggcaccg cccccaagtt tcccaccccc catcacatat
     2461 tgttccttct gcgctgctgg aagagaacca aaaacatcct gcccctggag atggccaaga
     2521 ccacccttca gggcatgagg atgggaggga tctacgatca tgtggggttc ggatttcaca
     2581 gatactccac cgatcccgag tggttcgtac cccacttcga gaagatgctc tatgaccagg
     2641 cgctcctggc catggcctat gcggaggcct atcaggctac gggtgaggag gagtacgccc
     2701 ggacggtgcg cgagatcctg gagtacatcc ggagggatat gacctctcca gagggaggct
     2761 tctactcggc tgaggacgct gatagcgagg gagaggaggg caaattctac acctggacag
     2821 cagttgagct aaaggaggcc ctgggcgaag aggatttcag gctattgatc cggctattcg
     2881 atgtctatga gagcgggaac tacgaggggg aaagaaacat cctccggcaa cgatcaagct
     2941 ttagcgatgc tgctagtgtc ctgaagatcc cggaggagga gctgtaccac cgctccagcg
     3001 atatgatatc ccgactctat ttggccagag agaggagggt ccatcccctc aaggacgaca
     3061 agatcctcac cgactggaat ggattgatga tcgctgccct ggcgcgggcg gcaggagccc
     3121 tgcaggatcc cgatctcgct atggcggcca gcagggcagc ggactttctc ttagaggtga
     3181 tgcggacccc cgaggggcgt ctgatgcatc gctaccggca gggagcagat attcaggcca
     3241 acctggatga ctatgccttc ctgatctggg gattgatcga gctgtatgag gcgacattcg
     3301 atgtcaagta tctgaaggcg gcagtccgtc tgaatgagat catggagcag cacttctggg
     3361 atggggaggc aggaggcttt ttcttcacgg cggatgatgg cgaggagctg ctggtcagaa
     3421 agaaggagta ctacgatggc gctctgcctt ctggaaactc cattgccctc ctaaacctcc
     3481 tcaggctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg atggcgctct
     3601 gccttctgga aactccattg ccctcctaaa cctcctcagg ctgctgcatt tgacgggaga
     3661 tacaagctta gaggggaagg ccgccctcct cgctcgctct gcccttcctg cagtctccgc
     3721 ccagccccta gggtatacaa tgcttctctg tgccctggac tatgctctgg gacctacgta
     3781 tgaggtagcc ctggccggca gcctggagga ggggggactg aaagagatgc tggccgcgat
     3841 aagaagcaga ttcttgccca ataaagcggt ggttttagca tcaggaagcg agatcgtcat
     3901 ggtcgctccc tttaccagag atctggtccc ggtcaaggga aaggccgcgg cttatgtctg
     3961 ctcggatcac gtctgccagc ttccagcaac cagtgcagca gagcttatgg ctctattaga
     4021 gaaaaattag catccgtctt gaatttggcg gaatgtgggt tctgggatat cctcgcgaaa
     4081 tacctctgaa ttccaggggt cgatttccat tccttgttcg aataaatcga atctgatccg
     4141 gacggaggat ttcagggatc ggttcaaagg catctggacg acaacgataa atactacatg
     4201 cggactagaa cgctcttcgg ggatttcaga tttcagagat tgaagttgca tccgatccgg
     4261 agcggcaccg tagtcacata aggcaccgta gggtaccgtc tggaaaaaaa cctaattggc
     4321 agcacaatgt ggaacttgtg gacgcaaatc tgtccaaagt aaaagaccta ttctctcaca
     4381 cgcagatcgt gcggtgatca tttaatgaac caacgacgtg atcagattcc taagaaggat
     4441 ataatgtaca ccttagtgga ggatgactct cagtacattg tcaatgagag ctacttctac
     4501 aagactgagc cgtactatgc catcgtcaag agtgagaatg agggaatcat aaccactccc
     4561 agtagcaggg atgttttaga cgcctatata gttcctatat gcctggagaa ggccaagctg
     4621 gcgggcatct ccgtatgcga ctggactgta tcctaccagt tcgtgcccct tccggccatc
     4681 atctatggcc tgaactactt ctccactccc agtgagtact tcctggtgaa tgatcatgac
     4741 gaggccaaga aggtggtaaa gcacatcacc aaccacggaa ggtatccttt ctgctaccag
     4801 aagattgctg atacttctgc cgttaccaga tgcgtctcca ttttcggcag gagcctgaac
     4861 tgctgtgaag aggtagcaga gattgcggag aagatctacc aggtattcaa gctgccctta
     4921 gtggagatcg taatggtgaa ggacaattct ggcacctata atctctcttc tctggcgccg
     4981 gtgaaatact ctcaattatc caaagaggaa aaagaaatgc tccaggacct tctggacaag
     5041 agggtgaaac gctttgagta aacttggaat cttcgtcaat agaaagacat tgagcagctc
     5101 ctctcagctc atggccctgg tgaagtgccg ggatgtggca gagagcatgg gccataccgt
     5161 cgagttcatc tttcccgtgg atatgaaaaa gattccccga ctggatgcgc tcttcatccg
     5221 tgccgacaca gatcccatga acaccaccta cgtggccgcc aggatggcgg agatgtacgg
     5281 cgtgccggtg atagacgacc ccacatccat caggatctgc gccgacaaga taaacatgta
     5341 catgcacctg cagaaagaag gcgtctccat acctaagacc aggtttctga agaaaagaga
     5401 attggatgac ctgcaggcaa ggcagctttt cgatgagctg ggcagcccac tgattctcaa
     5461 agagccgtca acatccttct ccgtgcgggt ggaaaaggtc tcggatgtgg aggagctctt
     5521 gaaggtggcc aagaggttct tcaagctatc cgactggatt gtggttcagg agtacatcga
     5581 gagccggttc gactggcggg tgggggtgat caatggccag ttgctttacg cctgccggta
     5641 tatcataccc tcggagacct tcaagatcca ggcttctgtc aacggccata tcgtctattg
     5701 cgacgtggag agcgtgcccg cggatcaggt gccgccagag gtaatagatc tgggaaagca
     5761 tgcggcaggg gctatcggta aaggattgta tggggtggac ataaaagaga gcaacggaaa
     5821 gctcttcgtc atagaggtga acgacaatcc ctccctggaa ggaggagagg atgaacatta
     5881 cccggagatg ttcggacaga tcatctctca tctcgtgtcc gcccaggctc tggacgatgc
     5941 ccgcgacctt tccatggaga cgatctcttc tgccggtctg atgggcagat atccgcagga
     6001 agcagaatcc gcctgtatac aggtcggcca attgggcgag agcgacatct actcgaagag
     6061 atgatatttg aatggatgag gacctttgcc aggccttgag ccgggcctgc gatgattgcc
     6121 atctggctgg tggctgctgc tttgaggcca ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnngattgcc atctggctgg tggctgctgc tttgaggcca ggcctcccct
     6301 gagccagaag cggatcgaca tgcttatgga aaacggcgtc agcgcggatg cgatcgagtt
     6361 tgtcggatat aagaggctcc gcctcaggca ggatggattc tgtgtgctct tccgggatgg
     6421 gaagtgcagc atccatgata tcaagcctga gacctgcgtg gccgggccgt tcacctttga
     6481 tattgagggt gatatgctgc agatattcct caagagggag agcatctgtc ccatggttcg
     6541 attcctaagg gctaaccgga aggcatacga tgccctcttc gagacctcag tggagaagat
     6601 catggatctg ataagaagcg tgcctgagga ggagatggcc cagattctac agatcgatga
     6661 gccccagact gatcttgtgg cggagatccg gctggaggac tgaaaaggaa tgccattgca
     6721 attcgggaga gtatccaggg cagcaatgca ccgggcacaa cccgcacaat ctcttttagg
     6781 aacggagcac gagtactcca taaacgatag caccttccgc cccctggcca tatccgacag
     6841 gatcatcgag agaataaatg gcagggtaga gcatgaggtg gccttcgggg gaattcgggt
     6901 gtccaaagag ctgcaaaagc atgtcctgga gcttattccg gcgtctcctg gcaaccttgg
     6961 ccgcctggag gagaatctct tttcgggact gcaagagctt ttccgggcga cgaaaaatga
     7021 gtaccagttc ctgggcctgg gaatgcatcc cctgctaacc ctggatcaga cgacctactg
     7081 ggatcacgat gagcaggact actaccaggc atacgaccgg ctctttggca tcaagcagca
     7141 tggctggctg aacatccagg cgctgcagat caatattcct tatagggatg agcaggagct
     7201 ggtggggatg ttcaacaaga ttcgctcgct catgccctat ctggtggcgg tgactgcctc
     7261 ctctcccttt gtcgagggac aagtcaattc ctatatggac agccggctgg tgtactaccg
     7321 aagaaatcag tcccagatcc cccagatctg ccggaatgtg attcccgaga ggctggaagg
     7381 aatcagagat tatgtcgaga tcaaccgcaa tatctatcgg gaattgaaaa agcagcatgc
     7441 tcagatactt tgccacgagt gggtgaactc cagaggagag atcgtgcgct tcaccaggag
     7501 gtgcctggag atcaaggcca tagatgagca ggagtgtctg cattcggata tggcgatctg
     7561 tgctttcatc atggccctcc tccgcactga cttggagctg gaggatgatg agagccatct
     7621 attagcgatg ctggaggatg gcatgcgtcg cggggtggcg gccatgaggc cggagctgga
     7681 gaggctctat gccctggcgg agaggaacgc cacccgggag gagcagaggt atcttcccct
     7741 gatcgaaaag agaatcgagc agggcagcct ggctgagatc atggtccaaa ggcaaaagga
     7801 ggagaaggag atcttgccca tgctctctca gctggcatgg tgcttgaagg agaatatccc
     7861 ttattctgct gagccgggac gttgcggatg agtgagatgc taatgaaagg cgttatttag
     7921 gcgaactggc aactggactg acagcagggg agaaggatct tgatcgccag gagagccgag
     7981 gaaattacgc ctttcattgt gatggaggtt ttagagaggg caagggagct ggagaggcag
     8041 ggagtggatg tcatccatct ggaggtgggg gagccggatt tcgatgtacc tttagcggtc
     8101 aagga
//
LOCUS       DAOSGP010000031         8100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_7620_length_8100_cov_19.212927, whole genome shotgun sequence.
ACCESSION   DAOSGP010000031 DAOSGP010000000
VERSION     DAOSGP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7620_length_8100_cov_19.212927"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLV49_01775"
     CDS             <1..314
                     /locus_tag="PLV49_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97599.1"
                     /translation="QSEASAFQFPEFADTNFDRLTVGNDRALAFGGIWRRPPLATATN
                     NLMIEKNQDSGECECCGPESCQECCLKVNMEQIRVGNREALAFGAAIATNNVKIVTSQ
                     K"
     gene            344..1075
                     /locus_tag="PLV49_01780"
     CDS             344..1075
                     /locus_tag="PLV49_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97600.1"
                     /translation="MKKMLKPTGLLLVGAIFIFSCLMLNALGSEAGLPDLQKEMKKTL
                     SGTLDKPYGSAIGKFKVNGETITLPWSVRGTSALRSKALFLWPLPSREDIALNETDSK
                     RVVSDLTAINTDREREIIYDGSQDCDAGSEDLSNSLEISVTGQETDDGSLNWYNGHVA
                     ADSIERAVDKALNRDKKNDLNSNNVTRSGSAALLGNNMIIDVSGISVSAINSVKGGSA
                     TATSNIVIRPVQIIACPSEVEEKLR"
     gene            1193..>2301
                     /gene="hisS"
                     /locus_tag="PLV49_01785"
     CDS             1193..>2301
                     /gene="hisS"
                     /locus_tag="PLV49_01785"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOG97601.1"
                     /translation="MRKRSQARKAMQEVLCRWGYQEVATPTFEHLELFTEKSGASVID
                     EIYSFQDKGGRDLALRPELTAPVMRMFVSELQNSPKPLRLYYFGNCFRYERPQKGRFR
                     EFWQLGAELIGGARPDSEAEAIALADQLIRSAGIRGDIHLGYLGLIRAILERIEGDKR
                     AAAMRMIDKKERSALKELLEDLDQGDLGLLELIDLKGKAALDKALEIAGDLLLTETQE
                     ASARSEGPKAFSEPKMQSEGRGSARNGRAVSSQLHLEEFQETVDLLGAYGVDFQIDFE
                     IVRGLDYYSGTVFEIYAEGLGAQRQICGGGTYELSSLFGGKETSSTGFGLGFDRIMEI
                     AHIKEERKPPVFLVYTPDVKVDAARIAVSLRRSVPT"
     assembly_gap    2302..2401
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2426..3709)
                     /locus_tag="PLV49_01790"
     CDS             complement(2426..3709)
                     /locus_tag="PLV49_01790"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG97602.1"
                     /translation="MSFGNLNIIDPLLRAIALEGYTTPTPIQTLAIPPLLEGKDLLGI
                     AQTGTGKTAAFVLPILQRMSAERRACRPGSPRVLVLAPTRELAAQIGQSFATYGKFLR
                     FRQLVIFGGVRQGPQVNMLSRGVDILVATPGRLLDLMNQGYIELKGVEFFVLDEIDRM
                     LDMGFIRDVKRIVSALPEKHSSLFFSATLTRETGELAQSLLNDPVHVQASPSACTLDS
                     IDQQVFFVDQNSKNSLLLDLLLKEDMKRVLVFARTKHRADKVAQMLSKNRIQADAIHG
                     NKSQNQRTNTMERFRSGRIRVLVATDIAARGIDIDDITHVVNYDLPNEPESYVHRIGR
                     TGRAGASGNAFSFCSAEERSFLKDIERLTREKIEEREHKYHSPRAKAATGADAKPLSP
                     ARSRVTRVQQRPVGGKVPGKQATSHRISHPAKVAC"
     gene            complement(4098..4277)
                     /locus_tag="PLV49_01795"
     CDS             complement(4098..4277)
                     /locus_tag="PLV49_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97603.1"
                     /translation="MFPLFLAANYILVKKMAAERRLRDDRLERAASDSVKESYSGKGL
                     PHQSWPKYKAARKKR"
     gene            complement(4345..>5001)
                     /locus_tag="PLV49_01800"
     CDS             complement(4345..>5001)
                     /locus_tag="PLV49_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG97604.1"
                     /translation="LCFQGARNLSLEQPEKVSYCLGGGPGEPPEIVALLSGVNSGGKT
                     SLLDLISQIVILAQMGLPVPAESCRISLFEQLYYFSKSRGTLSAGAFETAMRKFAVVE
                     NEKRKLVLADELESITEPGASARIIACMLDELNRRGSAAVFVSHLAEDVRRFSETPVR
                     VDGIEAEGLDENNCLVVCRSPRYNYLAKSTPELILDRLVRTTKGQEKEFYSRLLGKFR
                     "
     assembly_gap    5002..5101
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5102..6494)
                     /locus_tag="PLV49_01805"
     CDS             complement(<5102..6494)
                     /locus_tag="PLV49_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG97605.1"
                     /translation="MDLPGVGPRLRERLREHYGDEEKALQAVKGGDLLGLCSTLSERQ
                     ALQLVQYARALKFRVSPDEFLATDEAVRIYSLLISRLAGFAHTEYARLKIGTLFPSSS
                     QEMIEENRLAASRSLEVARRMEGLGIEEQLRSIRPLRERSASRVRERAVAASSAEDFQ
                     RLKSRGLDRLIDLHLAESPQELLALARSYSVVSLVDGGMVDADGVERAESLEDWYLVP
                     EAVLGFYRENMQILAAAVEAASKLRAAGVEDFQGWEDFFDLISRLGDKSDIEAERLSR
                     LAARLEGCVDQAASTANEELKRRIENSSLTLDGTDLLQVMSRGDGIRELFEIRMKGIY
                     QEVLKEAKERAKEDLDLKGAEEVWLDEIFSSAVSYPLELDRGALRSFEQELRSRQEER
                     GLKAKRELARGLAEERNRAEALVHILMEFDYSYALGSFTLKEGLSFPEIASETCLCFQ
                     GARNLSLEQPEKVS"
     gene            6634..7518
                     /locus_tag="PLV49_01810"
     CDS             6634..7518
                     /locus_tag="PLV49_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97606.1"
                     /translation="MLLNELLDLGEEESIFLAEESLQDLLDLPVTRQIMEEGVDWDRS
                     IELVDIGIALHLASPEDFWLLLTDRYCRERILMNAADRFGSEATKRIMVDESAFLRAL
                     VEYYAIFMKDELLCESCGTADELHYAEDRITRLVDLLKPNFSCSDEPILEICCGNGLA
                     TQALIRLGLDPLAMDSDRCELCIALKKGLMNPQRSFVLDARLLPRYFSGRPFHSVLGF
                     MVGLIDDSNWPLWREILLAAASLAEKELLFTVYSQKEADLIAKEMGKAGWRGEVIDNQ
                     DSRGIYDQWVYKGSRQSL"
     gene            7519..7698
                     /locus_tag="PLV49_01815"
     CDS             7519..7698
                     /locus_tag="PLV49_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97607.1"
                     /translation="MNDGKLNYPSHSSEEVVRFLADFKKRNAERKGGKKEEIKSKKLM
                     EENLDDLIGRGEVEK"
     gene            complement(7848..7988)
                     /locus_tag="PLV49_01820"
                     /pseudo
     CDS             complement(7848..7988)
                     /locus_tag="PLV49_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
BASE COUNT         1830 a         2170 c         2118 g         1782 t
ORIGIN      
        1 ttcagtcaga ggcatctgcg ttccagttcc ctgagttcgc agatacaaac ttcgatcgcc
       61 tgaccgtcgg aaatgacagg gctttagcct ttggcggcat ctggcggaga cctcctctgg
      121 ccacagccac caataacctt atgatcgaga agaatcagga ttcaggtgag tgcgaatgct
      181 gcggtcccga atcctgccag gaatgctgct tgaaggtcaa catggagcag atccgggtgg
      241 gaaataggga ggcactggcc tttggagccg ctattgccac caacaatgtg aagatagtca
      301 ccagccagaa atagatcggg atcatcagac ctgaaggacg aaaatgaaaa aaatgctaaa
      361 gcccactggc ctcttgctgg tgggggccat ttttatcttc tcatgcctca tgctcaatgc
      421 actgggatca gaggcgggcc tccccgacct gcagaaagag atgaaaaaga ccctctcagg
      481 cactctggat aaaccatatg gaagcgcaat agggaagttc aaggtaaatg gagagacgat
      541 cactctcccc tggtcagtgc gaggtacatc ggcgcttcgg tcgaaagcac tttttctctg
      601 gccattgcct tcaagagaag atatagcgct gaatgaaacc gactcgaaga gggtggtttc
      661 agacctgacg gcaatcaata ccgaccggga gcgagagatt atctacgacg gcagccaaga
      721 ctgtgatgct gggtcggagg acctttccaa ttccctggag atcagcgtca ccggccagga
      781 gaccgatgat ggatcgctaa attggtataa tggtcatgtt gcggcagata gcattgagcg
      841 cgctgtggat aaagcattga acagggataa gaaaaacgac ctcaactcga acaatgtgac
      901 caggagcggt tcggcagcgc tcttgggcaa taatatgatc atagacgtca gcgggatatc
      961 agtgagtgct ataaattcgg taaaaggggg aagtgccacc gccacctcca acatcgtgat
     1021 aaggccagtg cagatcatcg cctgtccatc tgaggtggaa gagaagctga gataattccc
     1081 atgatttctt ctttttgaat cacaaactat aagggcattt tgcgtctact cctctctcga
     1141 tatgaccatc atcaagaggc cgagaggcac gcgggacttc cttcctgaag agatgcggaa
     1201 gcggagccag gcccgaaaag cgatgcaaga agtgctttgt cgctggggct accaggaggt
     1261 agccactccc acatttgagc atcttgagct attcacagag aaatccggtg catcagtaat
     1321 agatgagatc tacagcttcc aggacaaagg aggtcgggat cttgccctcc gtccggaatt
     1381 gaccgctcca gtgatgcgca tgttcgtctc cgagctgcaa aactcgccca agcctttgag
     1441 gctctactac tttggcaatt gcttccgcta cgagcgccca cagaaagggc gcttcaggga
     1501 gttctggcag ctgggtgcgg agctgatagg aggtgccagg ccggattccg aggcagaggc
     1561 catcgctctg gcagatcagt tgatcaggag cgcgggaatc aggggagata ttcacctcgg
     1621 ctatctgggt ctcatacggg ctattttaga gagaatcgag ggggataaaa gagcggcagc
     1681 gatgagaatg atcgacaaaa aggagcggtc tgctttaaag gagctgctgg aggatctgga
     1741 tcaaggagat ctgggactgc tggagctgat cgatctgaag ggcaaagcgg ctttggataa
     1801 ggccctggag atcgccggag atctgttgct cactgagact caagaggcct ccgccaggag
     1861 tgagggtcct aaagcctttt ctgagcccaa gatgcagtca gagggccgcg ggtccgctcg
     1921 caatggaagg gcggtcagct cccagctcca cctggaggag ttccaagaga cggtagatct
     1981 tctcggagct tacggtgtgg acttccaaat cgactttgag attgtgcggg gcctggatta
     2041 ctactccggc acggtctttg agatctatgc cgagggtctg ggagcacagc gccagatatg
     2101 cggcggagga acatatgagc tctccagcct cttcggaggg aaagagacct catcaaccgg
     2161 cttcggcctg ggattcgacc gcatcatgga gatcgcccat ataaaggagg agagaaagcc
     2221 gccggtcttc cttgtataca cccccgacgt caaggttgat gctgccagga tcgcagtatc
     2281 tctgcgaagg tcagtcccta cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 ngagtgccag cccacctctc ctgattcagc aggcgacctt ggccggatgt gatatgcgat
     2461 gagaagtggc ctgcttgccc gggaccttac cgccgaccgg cctctgctga acccgggtga
     2521 cccttgacct ggcaggggat aagggctttg catccgcgcc agttgccgcc tttgcccgag
     2581 gagaatgata cttatgctct ctttcctcta tcttctcccg ggtaagcctt tcgatatctt
     2641 tcaagaagct gcgctcttct gcagagcaaa aggagaatgc attgccgcta gctcctgccc
     2701 tgccggtgcg cccgatgcgg tggacgtagc tctctggctc atttggcagg tcatagttca
     2761 ccacatgggt gatatcatcg atatctatgc cccgagcggc gatgtcggtc gccaccagca
     2821 cccttatcct gcctgatcgg aacctttcca tggtattagt gcgctggttt tgcgacttgt
     2881 tgccgtgaat ggcatccgcc tgaatcctgt ttttggacag catctgcgcc accttgtctg
     2941 cccggtgctt ggtgcgggcg aagaccagga ccctcttcat atcttctttt aggaggagat
     3001 cgagcagaag ggagttcttg gagttctgat cgacaaagaa tacctgctga tcgatggagt
     3061 ccagagtgca cgccgaggga gaggcctgca catgcaccgg atcattaaga aggctctggg
     3121 caagctcgcc cgtctcccgg gtcagagtgg cagagaagaa aagggagctg tgcttctccg
     3181 gcagagccga gactatcctc ttcacgtctc ttatgaagcc catgtcgagc atcctgtcga
     3241 tctcatccag gacgaagaac tcaaccccct tcagctctat gtagccctgg ttcatcaggt
     3301 ccaagagcct gccaggagtg gcgaccagta tatcgacccc ccgggagagc atattgacct
     3361 gcggcccctg gcgtacgccg ccgaatatga ccagttgtct gaagcggaga aacttgccat
     3421 aggtggcaaa gctctggccg atctgggcag caagctctct ggtaggagca agcaccagca
     3481 ctcgcggtga gcccggccgg caggctcgcc gctcggctga cattctctgc agtatgggca
     3541 gcacaaatgc cgccgtcttt cccgttcccg tctgagcgat ccccagcaga tcctttccct
     3601 cgagtaatgg gggtatggcc agcgtctgta tcggcgtggg ggtggtatat ccttctagag
     3661 caatcgcccg caagagcgga tctattatat tcagatttcc aaatgacatt taatgcctct
     3721 tctttgaatc tgaaaacaag atgaagtcca cggcaatagc tcgcaagata tgcaagcctg
     3781 ccggaagcag aggcattaaa cgacgatgga tgtctcccga agcgattgat ctcataatca
     3841 aaatcgttcc agagccatca gtctctttaa ggaagcctga tcagacagcc cgttttaggg
     3901 ccagagaaga agctgcctct gcggtttcaa atcatttcca ttatttattt gatttcctat
     3961 cgattcagcg aaccgatatc agcctcaaaa acatctcaac aatttatcga atcaatgcaa
     4021 atggagcggt tgagcatctc ctcaccagac taaatatctg tcggtcccat atatcggtca
     4081 atcttcagat ccatagatca tctcttcttc ctcgccgctt tatatttagg ccagctctgg
     4141 tggggcagtc ccttaccgga atagctctcc ttcacgctat ctgaggctgc cctctccagc
     4201 cggtcatcgc gcaacctcct ctctgcggcc atcttcttta ccaggatata gttggcggcg
     4261 aggaataggg ggaacaagag gatacagaat gctactatat gccagacttc catatataat
     4321 aggtcaattc atgaatataa aagatcatct gaacttcccc aagagtctag agtaaaactc
     4381 cttctcctgc cctttggtgg tacgaaccag ccggtccagt atcagctcgg gagtgctctt
     4441 cgccaggtag ttgtaccgcg ggctgcggca gactacaaga cagttgttct catccagccc
     4501 ctccgcctcg attccgtcca cccgaaccgg ggtctctgag aagcgacgca catcctcagc
     4561 caggtggctg acgaagaccg cggctgaacc ccttcggttc agctcgtcga gcatgcaagc
     4621 gatgatgcgc gcagaggctc cgggctcggt tatggactcc agctcatcgg ctaagaccag
     4681 ctttctcttc tcgttctcca ccacagcgaa ctttctcatc gccgtctcaa aggctccggc
     4741 ggagagggta ccccggctct tgctgaagta gtaaagctgc tcgaatagac tgatcctgca
     4801 gctttcggcg ggcacgggca gacccatctg cgccaggatg actatctgag agatgagatc
     4861 cagtaaagag gtcttgcctc cagagttgac tccgctgaga agggccacta tctctggagg
     4921 ctcgcccggc cctccaccca gacagtagct caccttttcc ggctgctcca atgagaggtt
     4981 tcttgctccc tgaaaacaca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nagctcacct tttccggctg ctccaatgag aggtttcttg ctccctgaaa acacaggcag
     5161 gtctctgatg caatctccgg aaagctcaaa ccctccttca gggtgaatga gcccagagca
     5221 taggaataat cgaactccat cagaatatgg accagcgctt ccgcccgatt tctctcctca
     5281 gccagaccac gggccagctc tctttttgcc ttcaggcccc tctcctcttg ccggctgcgc
     5341 agctcctgct caaaggatcg caatgctcct cggtccagct ccagagggta gctgacggca
     5401 gaggagaata tctcatccag ccagacctcc tcagctcctt tcagatccag atcctctttg
     5461 gcgcgctcct tggcctcttt caagacctcc tgatagatcc ctttcatccg gatctcgaag
     5521 agctcccgga tgccatctcc ccggctcatc acctgcaaga ggtccgtccc atccagggtg
     5581 agcgagctgt tctcgatcct tctcttcagc tcctcattgg ctgtagatgc tgcctggtcc
     5641 acgcaaccct ccagccgggc ggcaagcctg ctcaaccgct ccgcctcaat gtcgctctta
     5701 tcgccaagcc tgctgatcag gtcgaagaaa tcctcccagc cctgaaagtc ctccactccc
     5761 gctgcccgga gcttcgatgc cgcctccacc gcggcagcca ggatttgcat attctctctg
     5821 taaaagccta aaaccgcctc aggaaccagg taccagtctt ctagagattc ggccctctcc
     5881 accccatctg catccaccat acccccgtcc accaggctaa caacgctata gctccgggcg
     5941 agagccaaga gctcctgcgg gctttccgcc agatgaagat cgatcaatct gtccaggccg
     6001 cgggatttca gcctctggaa gtcctcagcg ctgctggcag ccaccgccct ctccctgacc
     6061 cttgaagctg acctctcccg caaagggcgg atggaacgaa gctgctcctc tatgcccagg
     6121 ccttccatcc tccgggccac ctccaaagag cgggacgcag ccaagcggtt ctcctctatc
     6181 atctcctggg aggaggatgg aaagagggtg ccgatcttca gtcgggcgta ctcggtatgg
     6241 gcaaaaccag ccagcctgga gatcagcagg gaatagatcc tgacggcctc atcggtggcc
     6301 aagaactcgt ctggagagac ccggaacttc agggcgcgag catactggac cagctgcaga
     6361 gcctggcgtt cgctaagggt actgcatagg cccagcaggt ccccgccctt caccgcctgc
     6421 agggctttct cttcatcccc ataatgctct ctgaggcgct ctcttaagcg agggccaact
     6481 cctggaagat ccaccagttt caagcgctgc cattttaaca ggaatataat atatctgttc
     6541 cgccaggcag aaggctgtca gaatctggag tggtcggccc ggacgggcct gcacggagaa
     6601 gattaatatt ccagaggcag aagatcaaat atcgtgctgc ttaatgaatt acttgatcta
     6661 ggcgaggagg agagcatatt tttggcggaa gagtccctgc aggacctgct cgacctgcct
     6721 gtaacccgac agataatgga ggaaggagtg gattgggatc gctctatcga gcttgtggat
     6781 atcggcattg cccttcacct ggccagtccg gaggatttct ggctgctttt aacagaccgg
     6841 tattgcaggg agaggatcct gatgaatgct gccgatcggt tcggatcgga ggcgacaaag
     6901 agaatcatgg tcgatgagag cgcctttctc cgtgccctcg tcgagtatta tgccatcttc
     6961 atgaaagacg agctgctatg cgagagctgc ggcactgcag acgagctgca ttatgctgag
     7021 gatagaataa cgaggctggt cgatctattg aagcccaact tctcctgctc tgatgagcct
     7081 attttagaga tctgctgcgg caacggactg gccactcagg ccctcattcg tctgggtcta
     7141 gatcccctgg ccatggactc cgatcgctgt gagctctgca tagccttaaa gaaggggctg
     7201 atgaacccac agaggtcctt cgtgctggat gccaggctcc ttccccgcta tttttccggc
     7261 aggccgtttc attctgtgct cggcttcatg gtgggtctga tcgatgactc caactggccc
     7321 ctgtggaggg agatactact tgccgcagcc tcgctcgccg aaaaggagct attattcacc
     7381 gtctactccc aaaaggaagc ggatctcatc gcgaaggaga tggggaaggc gggctggagg
     7441 ggtgaggtaa tcgacaacca ggactccagg gggatatacg accagtgggt ctacaaggga
     7501 agccgccagt cactttaaat gaatgatgga aagcttaatt atccatccca ttcttctgaa
     7561 gaagtggtga gattcttggc tgactttaag aagcgaaatg cggagagaaa gggcggcaaa
     7621 aaagaagaga tcaaaagcaa gaagctcatg gaagagaacc tggatgattt aataggcaga
     7681 ggagaagtag agaaataggc ttctgcagcg gagctttggg ccgaagagac caatggcttt
     7741 atttaacctt ttatcatcct ccgggaggaa gattttcgtc tcctgtgatc cccgggggag
     7801 tccccagttt gcaggtctga acggtcctcg agtgaacact ccccgcccta aagggcgggg
     7861 cttctagcat tttgcaaaga ttgttttccc aatcttaata tgttgattgc agcattccag
     7921 tctcgatcca ttgaaagact gcattgaggg caattgtgga ctcgatcaga aagagtcttt
     7981 tcgaccatta aggagtgacg ctcaagcttc ggctcaactc tttgagcttt tcgatgctct
     8041 ccagggtctt ggcggactct tttcgcttct catcctcgaa atgctttatg atatcgccca
//
LOCUS       DAOSGP010000032         8088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_7640_length_8088_cov_17.784265, whole genome shotgun sequence.
ACCESSION   DAOSGP010000032 DAOSGP010000000
VERSION     DAOSGP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7640_length_8088_cov_17.784265"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4542
                     /locus_tag="PLV49_01825"
     CDS             <1..4542
                     /locus_tag="PLV49_01825"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HOG97608.1"
                     /translation="LGSSVNWTAVASDPEDDPISYRFLVNETAVTDWQAENKFIWTAS
                     EAGSFLITAQARDDQHEGPEGESGNRSSEFVIMPLDEEAPFAEEPAEEIAPAETNVTD
                     QVNVTAPANITDEENATAQANMTAEAENVTEVVEIPEDTLAPPVTENITTPIAPENIT
                     ENLTEEVSEEDAEVVAPVVENQTPVLNSLIPDIISPQKPGVTVTWTANATDVDMDPLL
                     FRFFLNGPATNGAWEPKTEWISDDTWAWVTSSMDRGENQVKVQVRDGQHSGEDGFDSE
                     YSGYFTLAEPKMNISGLVYEDKNGNGVSDSGEALSGWTVQITKPDQSQVSVLTREDGT
                     YSFQDLDAGSYTISETLPSGWVAAIPEGGSHNVDLSEGDVEGLDFVNKLVQYTISGRK
                     FNDLNGNGAFDGEPGMEGWTIELSRDGSLISTATTEKDGSYKFAELSPGSYTVSEVEQ
                     AGWTRTAPPEGSYTVELTDGDVADIYFGNHGSFAISGTSFLDSNGNGVKDEGEPGRAG
                     VAIQLSRDGSVINATTTLEDGSYAFRNLSPGTYSISQVAAEGITQIAPEGPWTVELKD
                     ADVADKDFANRGGLSISGQKYYDINGNGLQDEDEPGIPGGEVSLVENGKVVANTTTDE
                     NGLYSFENVLPGTYTINDPVPTGMVLTTSSTVTVTIKTVVVTNVNFGIRGSNSISGMK
                     YEDLNGDGTKNPGENGLSGWEMVLTGSTWFGKPLPTLTATTDNNGNYKFERLLPGTYK
                     VSETSRTGWTQTAPAGGSHSIVFDVRTPPREAKNNDFGNRLAAQSISGVKYNDINGNG
                     VRDPGEPGMGGWKINLEPAGGGVIKVATTEADGSYSFTDVASGTYTVREIHQPGWDQK
                     APASGTYTVTLDSSTTSVSGKDFGNWNPLPANPSLIPDKSSPQRSGTPIIWTARADDL
                     DPLQYRFLVRGPGINLDTGYSSRSVWTWNTLGYPAGKYEVEVWIRDGQHSGPGGYDIK
                     KTVSFSLTSGNLPPSVQVLYTDRPEPQYAGSWIKWTAIASDPDRDPLQYRFFLRGPST
                     RGFWVDMTGWGKNNQWIWRTTPADVGYSEVLVAVRDGKHAGPAGSDDYQVGRFFIMNV
                     NLPPVITGLGTNLPSAQPVGATIRWAATASDPEGNPLFFRYWMRGPSTGGLWRMVRDW
                     STDPTWTWPTTPADAGTSEIKVQVRDGLHSSPAGWDDDAGALFTVRRQNLPPTLISLG
                     SDKPSSQKAGTPVTWTAIATDPDMEPLLYRFWLKGPSTGNSWKIVQDWSYKNQWTWAS
                     LPSDGGAYTVYVYVRDGWHNPETGYDSAVGAPFILALNQPPVLTALKSDRPSPQGAGT
                     PVKWTAIAIDADKDPIYYRFWLKGPSTGNVWQIAQDWSLANQWTWASLPNDGGAYTVY
                     VYARDGWHYPDTGYDSALGSSYQLISNQPPVLTALKADRPSPQGAGTPVKWTATASDA
                     DKDPLYYQFWLKGPSTGNVWRIVQSWSTMNQWTWSSAPTDAGNYRVYVYVRDGKHAPA
                     NAYDSAMGQGYMLQDMVRR"
     assembly_gap    4548..4647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4648..5807)
                     /locus_tag="PLV49_01830"
     CDS             complement(<4648..5807)
                     /locus_tag="PLV49_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG97609.1"
                     /translation="MQITKDSGSLWPLYLAVFVAVLGFTLVVPFFPSYVMDLGASYTL
                     LGFIISIYGASQLLVQVPIGRLSDRMGRKRLMLIGLMTFAVLPPLYIYAENAYTLLFI
                     RALGGVGAAFVWPTAMALIIDQSRAHSRGAAMGFYNAAFYSALALGPFMGGALYDQMG
                     LEAPFYFWTLLGIASIIIVTFKVPESRGIVLKADEQPQRREEPLIAPGYAITFLACCA
                     VILWSGVVGGFNITLLPSYAKALGFSTTDIGLIYLVYGGITAISNIYFGRLADRGRRK
                     WLIFLGSLAGLISFALLFIVSNLIQVLILFAGLGLGLGICSPAAAALVADTTNASRRG
                     ETYGIFNTARMSGVVVGPIMAGLAADMQGIDGALYAFTLIALAITLGAVAIR"
     gene            complement(5814..>6020)
                     /locus_tag="PLV49_01835"
     CDS             complement(5814..>6020)
                     /locus_tag="PLV49_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOG97610.1"
                     /translation="EPGTEKAVHDRLEKIKGICEVVPVYGERDFIAIVDVEGISDLNR
                     AVMNVREINGVTNTQTILGMELKF"
     assembly_gap    6021..6120
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6121..6587)
                     /locus_tag="PLV49_01840"
     CDS             complement(<6121..6587)
                     /locus_tag="PLV49_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG97611.1"
                     /translation="MNSKEKIQLHKGMDCVRRMDFEKAIEYFQIVTASNPDMPEAWNN
                     LGVAFYGLGRIDEALESYDRSIALDPSNLDALRNRAFLLRNQKRLPEALETYDTVLEK
                     GGDAMDLESTAVVLTAMGRLEEALNCLYLAREKLSLKRLEDEIEMVQKKILER"
     gene            complement(6594..6938)
                     /locus_tag="PLV49_01845"
     CDS             complement(6594..6938)
                     /locus_tag="PLV49_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOG97612.1"
                     /translation="MEVSVETTRSSEFKQIYAIGAIGGHSPYDFRIAFYNDSPKIQRD
                     GNKNITVMERKIEMEIILSPLAAKELARWLEEHIKDYEKKFGEIKRPGAGSGEKGNTE
                     KSGESAPIQGYM"
     gene            7055..>8088
                     /gene="carA"
                     /locus_tag="PLV49_01850"
     CDS             7055..>8088
                     /gene="carA"
                     /locus_tag="PLV49_01850"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOG97613.1"
                     /translation="MIGLLGLEDGTRVIGTGFGAPGVVAGELVFTTIMSGYEEALTDP
                     SYHGEMLLFTYPLQGNYGVSGDDFQSDGMWPRAMICREHWDRPTHHRLNKTLNQFLLD
                     EGRPGLCDVDTRMLTIKVREQGVLKAAVAVGEKDEIMDLDVVGLARSQPDISSQDLLR
                     DVTCKKPYRIEGRGKRIAVIDLGIKRRILKSLSDRDFDLMVLPAHSTIQDIEAAGPDA
                     LFLSNGPGDPERAEKAIQAVRHFAGEMPIFGICLGHQVISLALGAETFKLKFGHHGGN
                     QPVKDMEKGIVYITSQNHNFAVVPSSLEGTGLEVTQLNASDGTVEGIQSKSLDVIAVQ
                     YHPEAYPGPH"
BASE COUNT         1854 a         2175 c         2245 g         1614 t
ORIGIN      
        1 ctgggaagca gtgtcaactg gacagcagtt gccagcgatc cggaagatga tcccatatct
       61 tatcgcttcc tggtcaacga gactgcagtc accgactggc aggcagaaaa caagttcatc
      121 tggactgcat ctgaggccgg atcattcctg atcacagccc aggccaggga cgatcagcat
      181 gaggggccgg agggcgagag cggaaataga agctccgagt tcgtcataat gccgctcgat
      241 gaagaagctc cctttgcaga ggaacctgcg gaggagatcg ctcccgctga gacaaatgtc
      301 actgaccagg tcaatgtgac agctcccgcc aatatcacag atgaagagaa tgccaccgct
      361 caggccaata tgaccgctga ggcagagaat gtgactgaag tggtggagat cccagaggat
      421 actctggccc ctccagtgac tgagaacatc accactccca tcgcaccgga gaacatcacc
      481 gagaacctga ccgaagaggt atctgaagag gatgcagagg tcgtagctcc tgttgtggag
      541 aatcagaccc ctgtgctgaa ctctctgatt ccggatatca taagccccca gaagccaggg
      601 gtgactgtaa cctggaccgc caatgccact gacgtggaca tggatccgtt gctcttccgg
      661 ttcttcctca atggaccggc aacaaatgga gcctgggagc caaagacaga atggatttca
      721 gatgatacct gggcctgggt gacatcctca atggataggg gagagaacca ggttaaagtt
      781 caggttagag atggacagca ctctggcgaa gacggctttg actccgagta ctccggctac
      841 ttcaccctcg ccgagccgaa gatgaacatc tcgggcctgg tatatgagga caagaacgga
      901 aatggtgtgt ctgacagcgg cgaggcctta tccggctgga cggtacagat aaccaaaccg
      961 gatcaaagcc aggttagcgt cttaacccga gaggatggaa cctacagttt ccaggatctt
     1021 gatgctggaa gctacaccat cagcgagact ctgccctctg gctgggtggc agcaattccc
     1081 gaaggcggat ctcataacgt cgatctctca gaaggagacg ttgagggcct ggacttcgtc
     1141 aacaagctcg tccagtacac catctccggc aggaagttca acgacctgaa cggcaacggt
     1201 gccttcgacg gcgagccggg aatggagggc tggacgattg agctctctag agacggcagt
     1261 ctgatcagca ctgccaccac ggaaaaggac ggatcctata agttcgcaga gctatccccc
     1321 ggaagctaca ccgtatccga ggtcgagcag gctggctgga ccaggactgc acctcctgaa
     1381 ggctcctaca ccgtggagct gacggatggg gatgtagcag acatatactt cggcaaccac
     1441 ggttcctttg ccatatcagg aaccagcttc ctggacagca acggcaacgg cgtcaaggat
     1501 gagggcgagc cgggcagggc tggagtggcc attcagctat cccgcgacgg tagcgtcata
     1561 aatgccacca ccacgcttga agatggctct tatgccttcc gcaatctgtc tcctggaacc
     1621 tacagcataa gccaggtcgc tgcagagggc atcactcaga tagcacctga aggcccctgg
     1681 acggttgagc ttaaggatgc cgatgtggcc gacaaggact tcgccaaccg cggcggtctt
     1741 tccatctccg gccagaagta ctatgacatc aacggcaacg gcctgcagga cgaggatgag
     1801 cctggcattc ccggtgggga agtgagcctg gtcgagaacg gcaaggtcgt ggccaatacc
     1861 accaccgatg agaacggact ctacagcttc gagaacgtcc tgcccgggac ctacaccata
     1921 aacgatcccg tcccgacggg catggtcctc accacctctt ccacggtcac agtgaccatc
     1981 aaaacggtag tggtgaccaa cgtcaacttc ggaatacgcg gatctaattc catatccgga
     2041 atgaagtacg aggatctgaa tggcgacggc accaagaatc ccggtgagaa tggcctgtcc
     2101 ggctgggaga tggtcctgac cgggagcacc tggtttggca agccacttcc cacccttacc
     2161 gccaccacgg acaacaacgg caactacaag ttcgagaggc tccttcccgg cacatacaag
     2221 gttagcgaga cctcaagaac cggctggacg cagaccgctc cagcgggggg gagccacagc
     2281 atagtcttcg atgtgagaac acctcccagg gaggcaaaga acaacgactt tgggaataga
     2341 ttggcagctc aatccatctc tggagtgaag tacaacgata tcaacggcaa cggggtcaga
     2401 gatcccggcg agccaggaat gggcggctgg aagatcaacc tggagccggc tggaggaggg
     2461 gtgataaagg ttgcgaccac cgaagctgac ggctcttaca gtttcacgga tgttgcctct
     2521 ggaacctata ccgttcggga gattcatcag cccggatggg atcagaaggc accagcgagt
     2581 ggcacttata ccgtcaccct ggattcatcc actaccagcg tttctggaaa ggatttcggc
     2641 aactggaatc cattgcctgc caatccatca cttatcccgg acaagagcag cccccagagg
     2701 tctggaacac cgatcatctg gacggccagg gcagatgacc ttgatcctct ccagtacaga
     2761 ttcctggtta gagggccggg aatcaacctg gacaccggct atagcagcag gagcgtctgg
     2821 acctggaaca ccctcggcta tcctgccggc aagtatgagg ttgaggtctg gataagggat
     2881 ggacagcatt ccggcccagg aggatacgat atcaagaaga ccgtatcctt tagcctgacc
     2941 agcggcaatc tgccgcccag tgtgcaggtc ctctacaccg accgccctga gccgcagtat
     3001 gccggcagct ggatcaagtg gacagctata gcctctgatc cagacagaga ccccttgcag
     3061 taccggttct tcctgcgcgg tccttccacc cgcggattct gggtggatat gaccggctgg
     3121 ggcaagaaca accagtggat ctggaggacc actcctgcag acgtgggcta cagcgaggta
     3181 ctggtagcag tgagggacgg caagcatgcc ggacctgccg gctcggatga ctatcaggtt
     3241 ggccggttct tcatcatgaa tgtaaacctg cctccggtga tcaccggcct gggcacgaac
     3301 ctgcccagcg cacagccagt aggagctacc atcagatggg cggccaccgc cagcgacccg
     3361 gagggcaatc cgctcttctt caggtactgg atgaggggtc catcgactgg aggactgtgg
     3421 cgaatggtcc gcgactggtc caccgatcca acctggacct ggccgacaac gcctgctgat
     3481 gcgggcacca gcgagatcaa ggtgcaggtg agggatggcc tccattccag cccagccgga
     3541 tgggacgatg atgctggtgc gctgttcaca gtgcggcggc agaatctgcc ccccactctg
     3601 atctcccttg gatcggacaa acccagctcc cagaaggcag gaacgcctgt gacctggaca
     3661 gcaatagcga ctgacccgga tatggagcca ctgctttacc ggttctggct gaagggtcca
     3721 tccactggga acagctggaa gatagtgcag gattggtcat acaagaatca gtggacctgg
     3781 gccagcctgc catccgatgg cggagcttat accgtctatg tctatgtacg ggatggctgg
     3841 cataatcctg agaccggata tgatagcgct gtgggggccc ccttcatcct ggccctcaat
     3901 cagccgccgg ttctgaccgc tctgaaatcc gaccgcccca gccctcaagg agctggaacc
     3961 ccggttaagt ggacggcaat cgccattgat gcggataaag atccgatata ctaccggttc
     4021 tggttgaaag gcccctccac cggcaacgtc tggcagatag ctcaggactg gtccctggcg
     4081 aatcagtgga cctgggccag cctgcccaat gatggcggtg cctatacggt ctacgtctac
     4141 gccagagacg gctggcatta ccctgacacc gggtacgata gcgctctggg atcatcctat
     4201 cagctgatct ccaatcagcc gccggttctg accgctctga aggccgaccg ccccagcccc
     4261 cagggagctg gaactccggt taagtggacg gcaaccgcta gcgatgcaga taaagatcca
     4321 ctgtactatc agttctggct gaaaggcccc tccaccggca atgtttggag gatcgtgcag
     4381 agttggtcaa ctatgaatca gtggacctgg tccagtgctc ccaccgatgc tggcaactat
     4441 agggtctatg tgtatgtcag agacggcaag catgccccag ccaatgctta tgatagcgcc
     4501 atgggccagg gctatatgct gcaggatatg gtgagacggt aggagccnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnntcc cggatggcaa cagctcccaa agttattgcc
     4681 agggcgatca gagtgaaggc atacaaagcg ccatcaattc cctgcatatc cgccgccagc
     4741 ccggccatga tagggcccac caccactccg gacatacggg cagtattgaa gatgccatag
     4801 gtctcccccc tccgagatgc attggtggta tcggcgacga gggcggcagc cgccgggctg
     4861 catatgccca ggcccaaacc caggccggca aagaggatca gtacctggat gaggtttgag
     4921 actatgaaga gaagcgcaaa tgagatcagg ccagccaggc tgcccagaaa tatgagccat
     4981 tttctcctcc ctcgatctgc caggcggccg aagtaaatgt tggagatggc agtgattcct
     5041 ccatagacca gatagatgag ccctatatcg gtggtggaaa agcccagagc cttggcgtag
     5101 gaagggagaa gggtgatgtt gaagccgccc accacccctg accagaggat gaccgcgcag
     5161 caggcgagaa atgtgatcgc atatccaggg gcgatgagag gctcctctct tctttgaggc
     5221 tgctcatccg ccttcaggac tatgcccctg gattcgggta ctttaaaagt gactatgatg
     5281 atcgatgcta ttcccaggag ggtccagaag tagaagggtg cttcaagccc catctgatca
     5341 tagagcgctc cccccataaa tggacctaaa gccagggcgg agtaaaaggc agcattgtaa
     5401 aagcccatgg cggctcctct actgtgagca cgagactggt ctatgatcag ggccatggcg
     5461 gtgggccaga cgaaggcggc accgacaccc cccagagctc ttatgaatag cagggtatag
     5521 gcgttctcgg catagatgta cagaggtggg aggactgcaa atgtcatcag tcctatgagc
     5581 attagcctct ttcttcccat ccggtcggag aggcggccta taggcacctg gactagcagc
     5641 tgcgacgctc cgtagatgga tattatgaac ccgagcaggg tgtaagatgc ccccaggtcc
     5701 ataacatagc tgggaaagaa tggtacgacc agagtgaatc ccaggacagc gacgaatacc
     5761 gccagataga ggggccagag cgaaccggaa tccttggtta tttgcatcct gtcctagaac
     5821 ttcagctcca tgcccagaat ggtctgagta ttggtcactc cgtttatctc cctcacattc
     5881 atcactgccc ggttgaggtc ggagatgcct tccacgtcta ctatggcgat gaaatccctc
     5941 tcaccgtata cgggaacgac ctcgcagatc cctttgatct tctccaagcg atcgtggact
     6001 gctttctctg tccctggctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 tccctttcca ggatcttttt ctgcaccatc tctatctcat cctccaggcg ctttaaagag
     6181 agcttctccc tggcaaggta caggcagttg agggcctcct ccagccttcc catagcggtt
     6241 agaacaacag cagtagactc gaggtccatg gcatctcctc ccttttccag gacggtatcg
     6301 taggtctcca gggcctcagg tagcctcttt tggtttcgaa gcagaaatgc acggtttcgc
     6361 agagcatcga gattactcgg atcgagggca atagatcggt cgtagctctc cagagcctca
     6421 tcgattcgac ccaggccata gaaggccacg cccaggttgt tccaggcctc tggcatatcc
     6481 ggattggagg cggtgacaat ttggaagtat tctatggctt tctcaaaatc cattctccgg
     6541 acgcaatcca ttcctttgtg aagctgaatc ttttctttgc tgttcatgga tcatcacatg
     6601 tagccctgaa tgggggcaga ttctccggat ttttccgtgt tacccttctc gccggaccct
     6661 gcaccaggcc tcttgatttc cccaaatttc ttctcatagt ccttgatatg ctcttccagc
     6721 catctggcca gctccttggc agccaggggg gataagatga tctccatctc gatctttctt
     6781 tccatgacgg tgatgttctt gttcccatct ctctggatct tgggcgaatc attgtaaaaa
     6841 gcaattctga agtcataggg gctgtggccg cctattgccc cgatggcata gatctgcttg
     6901 aactcggagc tgcgcgtggt ctctacagat acttccatag accgaattat ggtctcaaga
     6961 ttaaaaaaga tgatgatctc tttgctgcga tctgatcgtc caactcatca tcgaaatgta
     7021 tttagacgac ctggcgtctt gttttcccga taatatgatt gggctcttag ggttagaaga
     7081 cggtacacgg gtgataggta ccggttttgg tgcgcccggc gttgtcgcgg gcgaactggt
     7141 cttcacaacc atcatgtccg gttatgagga ggcgctgacc gatccctctt atcatggaga
     7201 gatgctgttg ttcacttatc ctctccaggg taattatggg gtgagcgggg acgattttca
     7261 gtcggacggg atgtggccca gggccatgat ctgcagggag cactgggacc gtccgactca
     7321 tcaccgcctg aacaaaaccc tcaatcagtt cttgctggat gagggccggc ctggactgtg
     7381 cgacgtggac accaggatgc tcaccattaa ggtgagagaa caaggagtgc tgaaggccgc
     7441 ggttgccgtg ggggagaagg acgagatcat ggatctggat gtggtgggcc tggcccgatc
     7501 ccagccggac atatccagcc aagacctcct gagggatgtt acctgcaaaa agccctaccg
     7561 gattgaaggc cggggcaaga ggatcgcggt gatcgacctg gggatcaaga ggaggatatt
     7621 gaaaagcctg tcagataggg attttgatct gatggtgctc cctgcacata gcacgattca
     7681 ggatatagaa gcggctggac ctgatgccct gtttctgtcc aatggccctg gggacccgga
     7741 gagagcagag aaggccatcc aggccgtgcg ccatttcgct ggagagatgc ccatatttgg
     7801 catatgcctg ggccatcagg tcatatccct ggccctgggg gcggagacct ttaagctcaa
     7861 gttcggccat catgggggca atcagccggt caaagacatg gagaagggga tcgtctatat
     7921 cacctctcag aaccataact tcgctgtagt tccctcctca ctagagggga cagggctgga
     7981 ggtcacccag cttaatgcca gcgacgggac ggtggagggg attcagagta agagcctgga
     8041 tgtcatcgcg gtgcagtatc accctgaggc ctatcctggg cctcattg
//
LOCUS       DAOSGP010000033         7968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_7809_length_7968_cov_17.979148, whole genome shotgun sequence.
ACCESSION   DAOSGP010000033 DAOSGP010000000
VERSION     DAOSGP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7809_length_7968_cov_17.979148"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLV49_01855"
     CDS             complement(<1..491)
                     /locus_tag="PLV49_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOG97614.1"
                     /translation="MEWLERYRDLIPDFDAFRAVQSLALPSSCRINTLKIERYALLDR
                     LAENGIDYQSFNWYPLGLKLDMESPGKLAENLLGYIHIQEELSMVPPLVLDPQPGELV
                     LDLCASPGSKTCQISQMMKNLGRVIANEPSLARIAPLRSNCERLGVMNVAITRYDGRR
                     FPSR"
     gene            618..845
                     /locus_tag="PLV49_01860"
     CDS             618..845
                     /locus_tag="PLV49_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOG97615.1"
                     /translation="MFRGETGSSNGGSAPAVPVTAGEEYDVKIEDIAREGDGIARVEG
                     FVIFVADTKVGDDARVQIDKVMRRFAIGHKV"
     gene            876..1097
                     /locus_tag="PLV49_01865"
     CDS             876..1097
                     /locus_tag="PLV49_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOG97616.1"
                     /translation="MTGYDTDRSSLSAPAPVEVGGEYDVHIEDIAREGDGIARVEGFV
                     IFVADTKVGDDARIQIDKVMRRFAIGHKV"
     gene            1147..2025
                     /locus_tag="PLV49_01870"
     CDS             1147..2025
                     /locus_tag="PLV49_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97617.1"
                     /translation="MDPTLAQTGTDAFSSLLSNAWIIIFILLFLVPMLQRNYLQAERK
                     RVLARLGKARGSQVITLIHRQETISFLGIPLARYIDIEDSEKVLRAIRRAQKDIPIDI
                     ILHTPGGLALAATQIAMALKSHPAKKTVIVPHYAMSGGTLIAFAADQIIMDQHAALGP
                     VDPQLGDAQGAYPATSLLAVAEKKKIDEMDDKTLVYVEEARKALAQMRELLQKILVGK
                     CSDASKLDVIINEFVSGKYTHDHPFMAEDARALLGDCVQTDVPEEVYSLMELYRMEMG
                     RSRPGVEYVPLVRDRK"
     gene            complement(2063..2467)
                     /locus_tag="PLV49_01875"
     CDS             complement(2063..2467)
                     /locus_tag="PLV49_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG97618.1"
                     /translation="MVKMPADVKETLEKQKPVPIATASKLGVPNVVFVGLLKIIDDET
                     LMIADNFFYKTAQNLEENPKISILCYNGETKKSFQIKGTVTVSKEGVDYEAMKSWVHG
                     VNNKLPAKACAMVKVTEIYNAMWGPSAGKQIA"
     assembly_gap    2522..2621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2622..3580)
                     /gene="cobB"
                     /locus_tag="PLV49_01880"
     CDS             complement(<2622..3580)
                     /gene="cobB"
                     /locus_tag="PLV49_01880"
                     /EC_number="6.3.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenobyrinic acid a,c-diamide synthase
                     (glutamine-hydrolyzing)"
                     /protein_id="HOG97619.1"
                     /translation="MKRRSKSKGEWLEMGIKMRADCPRILLAGDRSSSGKTTIVAGLL
                     SALRGRNLKVQPFKVAMDYIDPSYHTWITGRSCRNLDGYLMNEAAVREIYAHAAQGAD
                     VAVIEGVRGLYEGYEGDLGSTAQIAKMLRVPVIFVVDARSITRSCAALVKGYMDYDPA
                     VQFRGVILNKVGSERHADKAIREIERYAKVEVVGTIRRNEDMHLAMRHLGLVPVLEGK
                     TRHEGFKERVDRIRQIVEEGLDLDRIREIAREAEPLPEIEPDLYLKNDCGKDLSIGVA
                     QDEAFNFYYRDNLELMELAGARIVPFSPVHDASLPEVDGIYIGG"
     gene            complement(3577..4392)
                     /gene="cfbC"
                     /locus_tag="PLV49_01885"
     CDS             complement(3577..4392)
                     /gene="cfbC"
                     /locus_tag="PLV49_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HOG97620.1"
                     /translation="MSSIKHVAIYGKGGIGKSCTASNIAAACAEEGHKVLMVGCDPKS
                     DSSITLIGKRIPTMMDLILKGGEFKEEDVVHQGFRGVRCIEVGGPEPGVGCAGRGIIV
                     AIDFLRKVSKAMEEVDLVLYDVPGDIVCGGFSAPIRKGLVSQAYIITSGEYMPLYAAN
                     NISKGLLRLNTPLAGVICNSREASGEREGEIVNQFAQELNSRMVAFIPKEPIVQQCER
                     RTVTVIEGAPDSKIAAVYRALAREIMSGGEARIPEPLSDERLRELSRDLGSEE"
     gene            4459..7383
                     /locus_tag="PLV49_01890"
     CDS             4459..7383
                     /locus_tag="PLV49_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein domain-containing protein"
                     /protein_id="HOG97621.1"
                     /translation="MNEIKKICFIVALLAAAINISLFCGAWAQDAAEFATVRGNLSYG
                     DGTWRANDFGWFYYDLDEDAGGEELQVKDVVGRTAKKGDITYLSRPWSKHFDYAPWGS
                     YEVLALFGKLYLASYPDSSFTDEVSSLAKGELREILRDEDTEYTLTYNTTLPLSQGYS
                     LTLGGISEKNDVVNFALYKNEERVYDSVVSIGEDFVFKMDDVPVILVHLANAMGSEDG
                     GFAEVNGIFQTSDSPIAKLVEGGTLGNMKLEDLSEDGLEFRNTVDVNLKRDSSVFLTQ
                     YLLLVVLDQPTLIYYPVGIIFDYGIQEIRGPAFSASTQIPVKLGDYDSSVTARWKSLN
                     YSGFYFDPEDMLGDETLVLYSVQDRRVSPPSNPVVYEENNTAFQKGFQYTTLLQPKEF
                     EFKPWGYYLVINFLGMQWFAGYDSSLMGTKASKSLLEKEYLGRVLIDEEYQGVAFVGN
                     YSLQEGYEIRIRDLENDSIFLELLKDGASIEGSVVRSNSTYIYEKDLGDVDDLPIIMV
                     HVDNVFNNISKGFATIDAIFQISDQFIIPVEEGNGIGEMQIVNVDPLGIMMVNDDYIN
                     LNRDSTVSLAPMMNIRVADNDTLRYYLYTQEYIVPSPSPPTIIVPDNVTSKTPASFTM
                     IEQAAEIREVTVEIVDSSNRTVFSRDITNFGQGSGEFWMFNWVWNATSLQLSDDNGRI
                     MDVGENLVPALLRLNQSSPEEQVGVYFNSAGRIGRILKSDSIYYVSRSEYEKLNVTSN
                     YDSMLANETIRKNFVKIELDLSSLRFQDIVNGRLDPSAKNHTLHGTIDSLEPHVRVAG
                     AEPGRYELRVRIENAVNAIQTRGDFFNVSRAPVRGISLGSAEISAGETASIPVEIPSS
                     SVERWINLTYDSNNAQALGISGNCSPSWQLEEALGRMRIIFPANCSKAELSFKAIEPN
                     EIIDINVTGWSGFEPEDITNGTITVLAGEGTADKAKKSNGPGYAAFLLAFAIAIYLRS
                     RS"
     gene            complement(7441..>7968)
                     /locus_tag="PLV49_01895"
     CDS             complement(7441..>7968)
                     /locus_tag="PLV49_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HOG97622.1"
                     /translation="MLQLSRRETKETCIDVVLETEGSGRIDVETEIELLDMILTSLGK
                     GANFDLRIKARGDSVTGDHHTIEDTGITLGKAIAQAKKSGIGSSTVPSSTALAVAAVR
                     FGETGYRGEFSFKSESSGGMTLENFGHFLRALAYNGGFTLVISAEGGDDMSKIEAMST
                     ALGRAIRRAWKDSEK"
BASE COUNT         1850 a         2061 c         2043 g         1914 t
ORIGIN      
        1 cgggagggaa atctgcggcc atcataacgg gtgatggcga cgttcatgac ccccaaccgt
       61 tcgcagttcg aacgcaatgg agcgattctg gccagcgatg gttcgttggc gatcaccctt
      121 ccaaggtttt tcatcatctg agagatctgg caggtcttgc tgccggggga ggcgcacagg
      181 tcaaggacaa gctcgcctgg ctggggatcg agcaccaggg gcgggaccat ggataactcc
      241 tcttgaatat ggatgtagcc aaggaggttc tcggcaagct ttcccgggct ctccatatcc
      301 agcttcaagc ccaggggata ccagttgaag ctctgataat cgatcccatt ctcggctaat
      361 cgatccagca gggcatatct ctctatcttc agggtattga tccggcagga gctgggaagc
      421 gccaggctct gcaccgcccg gaaggcatcg aagtccggta tcaaatccct atatctctcc
      481 agccattcca tatttacaag actagcattc tagaagaaat aaattggcct aattgagcaa
      541 aagaatttaa tagtcagatg ttgaggccaa gaccagcgca aaactgtttt ctggttttcg
      601 ctaaggacgg tgaaggcttg ttcagaggtg aaacaggatc ttccaatggc ggatccgctc
      661 cagctgtacc ggttacagcg ggagaagagt acgatgtcaa gatcgaggac attgcccgcg
      721 agggcgatgg cattgccaga gtggagggat ttgtgatctt cgtggccgat accaaggtag
      781 gcgacgatgc ccgggtccag atcgataagg tgatgagacg atttgccatc ggccataagg
      841 tctgaatttc ttttctagtt ggattggtga tttaattgac aggttatgat acagataggt
      901 cctctctgag cgcaccagct ccagtagagg ttggaggaga gtatgatgtc catatcgagg
      961 acattgcccg cgagggcgat ggcattgcca gagtggaggg atttgtgatc ttcgtggctg
     1021 acaccaaggt aggcgacgat gcccggatcc agatcgataa ggtgatgagg cgatttgcca
     1081 tcggccataa agtttagcag aaaataaata ttaaatacta tccaggccaa ttgatcctag
     1141 atactgatgg atccaacttt agctcagacg ggcacggatg cttttagcag cctgcttagt
     1201 aacgcctgga taataatatt catactcctt tttctggtgc cgatgctcca gaggaattat
     1261 cttcaggcgg aaagaaagcg cgtcttagcc aggttgggca aagccagagg cagccaggtc
     1321 ataaccctca ttcatcgcca ggagacgatc agtttccttg gcattcctct cgcccggtac
     1381 atagatatcg aggacagcga gaaggtttta agggctatcc gtcgggcgca gaaggatatc
     1441 cccatagata tcattcttca tactccggga gggctggccc tggccgccac ccagatcgcg
     1501 atggctctga aatcccatcc cgccaaaaaa acggtaattg tgccccatta tgccatgagc
     1561 ggcggaacgc tgatcgcctt tgccgccgac cagatcatca tggaccaaca tgctgccctc
     1621 gggccggtcg atccgcaatt gggcgatgcc cagggcgctt accccgccac atccctcctg
     1681 gcggtggctg agaagaagaa gatcgatgag atggacgata aaactctggt gtatgtggag
     1741 gaggccagaa aggctctggc ccagatgaga gagctcttgc agaagatcct ggtgggcaaa
     1801 tgcagtgatg cctctaagct ggatgtgatc ataaatgagt tcgtatccgg aaaatatacc
     1861 cacgaccatc ccttcatggc cgaggacgca agagccttgc tgggggattg cgtgcagacc
     1921 gatgtgccag aagaggtcta ttccctgatg gagctttacc gcatggaaat gggcagaagc
     1981 cggccagggg tggagtacgt gccgctggtg agagatcgaa agtgaaaaag tgcaatgatg
     2041 gacgggggat agcccatcat tttcaagcta tctgctttcc tgcagacggt ccccacatag
     2101 cgttgtagat ctcagtgacc ttgaccatgg cgcaggcctt ggccgggagc ttgttgttga
     2161 ctccgtgcac ccaggatttc atggcctcat agtccactcc ctccttggag actgtgacag
     2221 tgcccttgat ctggaaggac ttcttggtct cgccattgta gcacaggatg gatatcttgg
     2281 ggttctcctc caggttctga gcggtcttgt agaagaagtt gtccgcaatc atcagcgtct
     2341 catcatctat gatcttgagc agtccgacaa ataccacatt cggaactccc aatttgctcg
     2401 cagtggcaat gggaacgggc ttttgcttct ccagggtttc tttcacatcg gctggcattt
     2461 taaccatgat aattcacctt tgtgctaata gaagtccgca ttcaataaat acctgagctt
     2521 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgcctatg tagatcccgt
     2641 cgacctctgg cagagaagcg tcatgcaccg ggctgaaggg gactatcctc gctccagcca
     2701 gctccatcag ctccaggttg tcgcggtaat agaagttgaa ggcctcgtcc tgagcgacgc
     2761 ctatggagag atccttgccg caatcgtttt tcagatacag gtctggttct atctcgggca
     2821 agggctcggc ctcgcgggct atctccctga ttctatccag atccaggcct tcctccacga
     2881 tctgccggat ccggtccacc ctctccttga atccctcgtg gcgggtcttc ccctccagca
     2941 ccggcaccag gcccaggtgg cgcatggcca ggtgcatatc ctcattgcgc cggatagtgc
     3001 ccaccacctc cacctttgca tagcgttcga tctcccttat cgctttgtcc gcatgccttt
     3061 ccgagccaac cttgttcagt atcacccccc ggaactgaac ggcaggatcg tagtccatat
     3121 accccttgac cagggcagca cagctgcggg tgatgctgcg cgcgtccacc acgaagatga
     3181 ccggcactcg gagcatcttg gcaatctgag cggtcgagcc gagatctccc tcatatccct
     3241 cgtaaaggcc acgcaccccc tcgatcaccg ccacatccgc cccctgcgca gcatgggcat
     3301 agatctcgcg gacggcagct tcgttcatca gatagccatc caggttgcgg cagcttcggc
     3361 ctgtgatcca ggtatgatag ctgggatcga tgtagtccat ggccaccttg aagggttgaa
     3421 ccttcagatt ccggccccgc aaggcggaga gcaagcccgc tactatggtc gtcttgccgc
     3481 tggagcttct gtcccctgct aagaggatac ggggacagtc tgccctcatt tttatgccca
     3541 tctcaagcca ctctcccttg cttttgcttc ttcttttcac tcctcagagc ccagatcgcg
     3601 ggagagctct ctcagcctct catcgctaag aggttcaggg atcctggcct ctcccccgga
     3661 catgatctcc cttgccaggg cccggtagac agcggcgatc ttcgaatcgg gcgcgccctc
     3721 gatcacagtc accgtcctcc tctcgcactg ctggactatc ggctccttgg gaatgaaggc
     3781 caccatccgg ctgttcagct cctgggcgaa ctggttgact atctcaccct ctctttctcc
     3841 agatgcctcg cgagagttgc atatcacccc tgcaagaggg gtattcaatc gcaagaggcc
     3901 tttgctgata ttgttcgccg cataaagggg catgtactcg ccagaggtta tgatgtaggc
     3961 ctggctcacc agccctttcc tgattggagc ggagaatccc ccgcagacga tgtcccctgg
     4021 cacatcatag agcaccagat ccacctcctc catcgctttt gataccttcc gcaaaaaatc
     4081 aatggcaacg attatgcccc gtcccgcgca tcccacgccc ggctcgggcc cgcccacctc
     4141 aatgcacctg actcctctga agccctgatg gaccacatcc tcctctttga actctcctcc
     4201 cttgaggatg aggtccatca tcgtgggaat tctctttcca atgagggtga tgcttgagtc
     4261 gctcttcgga tcgcagccca ccattaaaac cttgtggcct tcctcggcac aggcagcagc
     4321 gatgttcgag gcagtgcaag acttgccgat accgcctttg ccatatatcg caacgtgttt
     4381 aatagatgac atcttactcc ggttggtttg tggtttgcca taggatatac agcttatcca
     4441 ggacaggttc acagagaggt gaatgaaata aaaaagatct gtttcatagt tgcgcttctg
     4501 gctgcagcaa taaatatcag ccttttttgc ggtgcatggg cacaggacgc agcagagttt
     4561 gctaccgttc gaggcaatct cagctacggc gatggaactt ggcgagcgaa cgacttcggc
     4621 tggttctatt atgatctgga tgaggacgca ggtggagaag agctccaggt aaaagatgtg
     4681 gttggaagaa cagcgaaaaa aggcgatatc acctacctct ccaggccatg gtcgaagcat
     4741 tttgattatg ctccctgggg gtcctatgag gttttagcgc tatttggcaa gctctatctg
     4801 gccagctatc ctgatagttc ttttaccgat gaggtcagct ccctggccaa aggagagctt
     4861 agggagatat taagagacga ggataccgaa tatactctga cctataatac gactcttcct
     4921 ctctcacagg gctattccct gaccttgggc gggatctctg agaagaacga cgttgtgaat
     4981 ttcgctctct ataaaaatga agagcgcgtc tacgactcag tggtgagcat tggcgaggac
     5041 ttcgtcttta aaatggatga tgtacctgtc atcctggtcc atctggccaa tgccatgggc
     5101 agcgaagacg gtggatttgc tgaggtgaac gggatcttcc agaccagtga cagtcccatc
     5161 gccaagctcg tcgagggagg aaccctggga aatatgaagc ttgaagatct atctgaggat
     5221 ggtctggaat ttcgaaatac cgttgatgta aaccttaaga gagactcatc tgtattttta
     5281 acccagtatc tgcttttggt cgtcctcgat cagcctacgc ttatatatta tcctgtgggc
     5341 ataatattcg actacgggat ccaagagata agaggaccag cattcagtgc cagcactcaa
     5401 atcccagtga agctgggaga ctatgattct tctgtcaccg ccagatggaa atcattgaat
     5461 tacagcggat tttattttga tccagaagat atgctggggg atgagaccct ggttctctat
     5521 tccgtccagg acaggagggt atctccgcca tccaatcctg ttgtgtatga agagaataat
     5581 actgcatttc agaagggctt ccagtatacc acgctccttc agcccaagga atttgagttc
     5641 aagccctggg gatactattt agttataaat ttcctgggaa tgcaatggtt tgcaggatac
     5701 gacagcagcc tgatgggcac aaaagcctca aagagcttgc tggagaagga gtatctgggc
     5761 agagtgctca tcgatgaaga gtatcagggt gtcgcatttg ttggcaacta ttctctgcag
     5821 gaaggctatg aaatccgtat tcgcgacctg gaaaacgata gcattttcct ggagcttctg
     5881 aaggatggag catcgattga aggctctgtg gtaagatcca actccaccta tatctatgag
     5941 aaagatctgg gcgatgtcga cgacctgccg atcataatgg tacacgtaga caatgttttc
     6001 aacaacatat ccaagggctt tgccaccatc gatgccatct tccagatatc cgatcaattt
     6061 atcattcctg tggaggaggg caatggcatt ggagagatgc agattgtgaa cgtcgatcca
     6121 cttggcataa tgatggtcaa cgatgactat atcaatttga accgcgactc gaccgtctcc
     6181 ttggctccaa tgatgaatat ccgggtggca gacaacgata ccctgcggta ctatctttat
     6241 actcaagaat atatcgtgcc atctccaagc cctccaacca taattgtgcc agacaatgtg
     6301 acctcaaaga ccccggctag cttcaccatg attgagcagg cggcagagat aagagaggtc
     6361 actgtggaga ttgtggactc ctcaaaccgg acggtctttt ccagagatat cacaaacttc
     6421 ggccagggat cgggcgagtt ctggatgttc aactgggttt ggaacgccac atcccttcag
     6481 ctcagcgacg ataatggccg gattatggac gtgggggaga atctggtgcc agcattgctg
     6541 cgcttgaatc agtcctctcc agaagagcag gttggtgtat acttcaattc cgctgggagg
     6601 atcggcagga tactgaaaag cgattcaatc tactatgtat ccagaagcga gtacgagaag
     6661 ctgaacgtca cctcaaatta cgattcaatg ctggccaatg agaccattag gaagaatttt
     6721 gtcaagatcg agctcgatct aagctctctg aggttccagg acattgttaa cggcagactg
     6781 gaccccagcg ccaaaaatca taccttgcat ggtacgattg actctcttga gcctcatgtg
     6841 agagtggcag gagcagagcc cggccggtat gagctgaggg tgaggataga gaatgctgtc
     6901 aatgctattc agacaagggg cgattttttc aatgtctctc gcgcccccgt gcgcggcata
     6961 tccctgggct cagcagaaat ttctgcgggt gagactgcct ccattccagt cgagattcct
     7021 tcatcctctg tggaaagatg gatcaatttg acatacgatt caaacaatgc ccaggctctg
     7081 ggcatctcgg gcaattgcag cccctcctgg cagctggagg aggccctggg aagaatgaga
     7141 atcatcttcc ctgccaactg cagcaaggcg gaattgagct ttaaagcgat tgagccgaat
     7201 gagataatcg atatcaatgt aactggatgg agcggttttg agccggagga tatcaccaac
     7261 ggcaccataa ccgttcttgc tggagaaggt actgcggata aagccaagaa gagcaatggt
     7321 cctggttatg ccgcatttct tctggctttt gccatagcaa tctatctgag gagcaggagc
     7381 taggcaagat gcaagagcca gggcaggcct ccctggttat tctttttctg caatttgata
     7441 ttatttttca gagtccttcc aggcccttct gatcgctctg cctaaagcgg tgctcatggc
     7501 ctcgatcttg ctcatatcat ccccaccttc agcgctgatg accagggtga aacctccatt
     7561 gtaagccaag gcgcgcagga agtgtccgaa gttctccaga gtcatgcctc ctgagctctc
     7621 gctcttaaag ctgaactcgc cccggtatcc ggtctcgccg aaacgaactg cggccactgc
     7681 cagggcagtg ctcgaaggca cagttgagct gccaatgccg cttttttttg cctgagcaat
     7741 cgcctttccc agggtgattc ccgtgtcctc aatggtgtgg tggtcgccgg taacagaatc
     7801 tcccctggcc ttgatcctca gatcgaaatt agctcctttc cccaaagagg tcaagatcat
     7861 gtccaaaagc tcaatttccg tttcgacatc tatcctgcct gagccttccg tctccaggac
     7921 tacatcgatg catgtctctt tcgtctctct cctactcaac tgcagcat
//
LOCUS       DAOSGP010000034         7946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_7843_length_7946_cov_17.604359, whole genome shotgun sequence.
ACCESSION   DAOSGP010000034 DAOSGP010000000
VERSION     DAOSGP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7843_length_7946_cov_17.604359"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..433
                     /locus_tag="PLV49_01900"
     CDS             86..433
                     /locus_tag="PLV49_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOG97623.1"
                     /translation="MGAIVPIAALAAIKIIAPNKPSRQKLSIYEGGLKPIRDAKIQYS
                     VQYYLFAIVFVIFDVEVLFLYPWIYVYANEAMQKFMVFGSMNIAVFEMLLFIIVLLVG
                     LIYAIKKEALRWV"
     gene            424..996
                     /gene="nuoB"
                     /locus_tag="PLV49_01905"
     CDS             424..996
                     /gene="nuoB"
                     /locus_tag="PLV49_01905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOG97624.1"
                     /translation="MGISDVIPVPVCIDEEIEKWTIFGQPVTEVWFTTTEKIHEIIQM
                     GPIKNIFNWGRKNSVYFLMQPMGCCGVEMFVFGAAPYDSDRFGCIPRNTPRQTDVMII
                     SGYLTRKYLPVFKNLWENMPEPKWCIAIGECAISGGPFYDSYNIVQNTGDYFPIDVYI
                     PGCPPRPEQFLEGLMNLQEKIKQRKDLRDY"
     gene            1011..2723
                     /gene="fpoD"
                     /locus_tag="PLV49_01910"
     CDS             1011..2723
                     /gene="fpoD"
                     /locus_tag="PLV49_01910"
                     /EC_number="1.12.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051911 - Methanosarcina-phenazine
                     hydrogenase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F420H2 dehydrogenase subunit FpoD"
                     /protein_id="HOG97625.1"
                     /translation="MTNASEVLSSIQSAFPGAVSDARVESDNRLWATIDSKKIRDVCK
                     YMTDKLGFDHYAGSAGVDWIARNEMEVVEIMASYGVHKVVAMLKVKTPRDNPSVPSMV
                     GMYWNANWYEREIWEMFGINFDGHPELYPLLLSDPLVGSWPWRKDFKGYPDLTTGERA
                     VQITTPEGYTEYHFPPTPAEVEAGTVVTERPKYPTFREREEAQIKSDSEMIMHLGPQH
                     AMVPGPFLLDILVEGERVKKAFLDIGYIHKGIEKIMENRTWLQGITYTDRMCYVASLT
                     NNECYCGAVERLLGLEVPLRAQYIRVILEELSRIQSHLIGTGEFLTLIAGVGFAPWQY
                     MIMDREKIISLIESVTGARLTHSFVRFGGVRNDLPEGFDEHCRKVFPYMKSRIEEFIE
                     LFAQDPIYHARMENIGSISPETAKRIGCAGRVLRAAGVPYDMRIEDPILVYPELDFKV
                     VTGTRGDSADRIDCTLREMLESIHIIEQCLDRIPSGPIKTEAKIPKKIPAGEAYYRVE
                     DPRGEMGMYVISDGGDKPYRVKVRGPFYATFQTLTPLLEGVYIADAVAIAGSMDGCPS
                     ESDR"
     gene            2736..3788
                     /gene="fpoH"
                     /locus_tag="PLV49_01915"
     CDS             2736..3788
                     /gene="fpoH"
                     /locus_tag="PLV49_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420H2 dehydrogenase subunit FpoH"
                     /protein_id="HOG97626.1"
                     /translation="MDGIIDTITQFAAQEPAIFALIIGIIGAAVLSAVVNVGAMLVIW
                     ADRKVMSDFCNRFGPNRVGGRWGILQLGADAIKLFTKEDVIPAGVDKGVYVWAPIIAS
                     AGTMLVAAAIPFGALHIGGKNYPLVVANMDISAFYVEAALSIMSIAVFMAGYSSNNKY
                     SILGAFRGIARMIAYEVPMGVCVISVAIMAHSLNLVEIVESQTVWYAFAQPLGFVIFM
                     IALVTDLGRVPFDQSEAEEEIICGYSTEYSGIRWGLLYFQEYNNFLLGSILVSLLFLG
                     GWHGPIIPIPLLDIVSPLIWLLMKVIIVIWFIILIRAALFRLRIDQVTDLGWKWMLPL
                     SILNLGWAVIVGSYFA"
     gene            3801..4388
                     /locus_tag="PLV49_01920"
     CDS             3801..4388
                     /locus_tag="PLV49_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG97627.1"
                     /translation="MLKRKMIKNFTWPLKTASREIEVTRLYPEFMMELPDAERGIHEL
                     DATICIGCGSCARVCPNSCIEMVHFKFGNPLKNKKMQFPQIDYGRCMFCGLCVDECPV
                     ECLKMGKKVIMAGWERKDIVKGPDFLATKRFSAKEAADLEAEAKRIAAEKAAAKKAAA
                     KDASAAGDKKPAKEGANAEKKKKNAEAKPAEGGAS"
     gene            4388..4819
                     /locus_tag="PLV49_01925"
     CDS             4388..4819
                     /locus_tag="PLV49_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOG97628.1"
                     /translation="MSEKGPEKNVIRSIFELLVLLLALGVIFGGLAVIVLLSPWSKAI
                     LDRLLDYDIRFAIELLAFLAIATIIVLLSALTVLVKNIVHSALYLLGTFAGVAALYIF
                     MNAPFVGVAQILVYIGAVGVLILFAVMLTRRTIMEESHGEI"
     gene            4806..5069
                     /locus_tag="PLV49_01930"
     CDS             4806..5069
                     /locus_tag="PLV49_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOG97629.1"
                     /translation="MAKFKLIILTLAFLAALVASLSVTDWGPVTEHPLGYAPSEGMRL
                     PESGVGDIGFEIFTTYVFSFEVLALVLTAALVGAIWVARKEDA"
     gene            5108..5410
                     /gene="fpoK"
                     /locus_tag="PLV49_01935"
     CDS             5108..5410
                     /gene="fpoK"
                     /locus_tag="PLV49_01935"
                     /EC_number="1.12.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051911 - Methanosarcina-phenazine
                     hydrogenase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F420H2 dehydrogenase subunit FpoK"
                     /protein_id="HOG97630.1"
                     /translation="MIPLELYILLASIMFTIGLYGLVTQKNGVKLMMCIELLLNSANI
                     NLVAFSAYQPNMNGQVFALFSIALAAAEAGVGFAILIALYRLYGTIDLDNINTMRW"
     gene            5414..7486
                     /gene="nuoL"
                     /locus_tag="PLV49_01940"
     CDS             5414..7486
                     /gene="nuoL"
                     /locus_tag="PLV49_01940"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOG97631.1"
                     /translation="MYSDLAYLIVGLPVLAFVLCLFIGSRLPRGGGFLTVLATLGGLI
                     ISLGIFQEIYPRGIIHQSMRWFGDFNVGILIDPLALVMLLMVTFVVMLIHTYANGYMN
                     GDPGANRYFAEAALFTASMLGLIYADNILQLFIFWELVGLCSYLLIGFWYRKPSAAAA
                     AKKAFLTTRVGDVMFLAAIIVLYNNLASLNLTLNPGEYLLQFPVIYANLALIPPDQLT
                     LIALGFLGGAVGKSGQFPLHVWLPDAMEGPTTVSAMIHAATMVTAGVYLVARMFPLFY
                     AAPHGLTAVAAVGAFTALFAATMGLTAFDIKRVLAFSTVSQLGFMMAGLGVGAAVGAF
                     SVGVSIFHLIGHSFFKALLFLCAGSVIHAVNTNDMREMGGVGKYMKWTMYTMLIGSLS
                     LAGFPLFTGFFSKDEIIVIAYEYGVAINFLPYIFLVLAAVLTAIYTFRMWFSVFTGKE
                     RSNYGKHESPWVMLGPLVILAVFALAFGFVAQEGFYNYVGDNFGQYEEKFGVDVNFAE
                     LALIGGHEVLGEHGAPAEGEHPSAEAEEGHGAAGEGEAGGEEAEGEPWYIQILPIFIA
                     IGGILIAALFYWDRAKKFDPSQVTGDKDPIRKMLLKGYYQHEIMTGWISEGIVYGTAL
                     ICNTIDIKVVDGWLNWLSAWVMGFAGHVRKVQTGVVQNYVTALMLGIVVLVIAIAAIR
                     LAMEVGLI"
     gene            7483..>7946
                     /locus_tag="PLV49_01945"
     CDS             7483..>7946
                     /locus_tag="PLV49_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOG97632.1"
                     /translation="MISNALSMMILIPLLGAIVSFFVGTKAKFVALIASFAPLVLSLQ
                     MYMEFDKSSTMMQFVESYDWVPSIGVKYTLGVDGIGFPLVLLSTIVTILVIIYSWGET
                     KKPNQYFALLLLNEVGVLGVFTALDFFLFYIFWEIVLIPMFFLIGSWGGPRK"
BASE COUNT         1852 a         1924 c         2189 g         1981 t
ORIGIN      
        1 tatgtttagc ttttacggag gataaacaat ggccggcgaa ccgatggcag tggtttatta
       61 tataccactt attatatttt tgataatggg agctatagtg cccattgcgg ctctggcagc
      121 tattaaaatc atagcgccga ataaacctag ccgccaaaag ctatcgatat acgaaggagg
      181 gctgaaacca atccgcgacg ccaagatcca gtatagcgtt caatattacc ttttcgcaat
      241 cgtttttgtg atattcgatg ttgaggtttt gttcttatac ccctggatct atgtctacgc
      301 gaacgaagcc atgcaaaagt tcatggtctt cggatcaatg aatattgccg tcttcgagat
      361 gctcctattc atcattgtat tgctggttgg actgatttat gcaatcaaga aggaggcttt
      421 gcgttgggta taagcgatgt catacccgtg cctgtatgta tcgatgagga gattgagaag
      481 tggacaatat tcggccagcc tgtcactgag gtctggttca ccaccactga gaagatccat
      541 gagatcatcc agatggggcc catcaagaat atcttcaact ggggcagaaa gaattcagta
      601 tactttttga tgcagcccat gggctgttgt ggtgtggaga tgttcgtctt cggcgctgca
      661 ccctatgaca gcgaccggtt tggatgcatt ccgagaaaca ccccccgaca gacggatgtg
      721 atgatcatct ccggttactt gacccggaag tacctgccgg tcttcaagaa cctgtgggag
      781 aacatgcctg agcccaaatg gtgcatagcc ataggcgagt gcgccatatc cggtgggccg
      841 ttctacgatt cctacaacat cgtgcagaat acaggcgact atttccccat tgatgtctac
      901 atccccggat gtccaccacg ccctgagcag ttccttgagg ggctcatgaa tctccaggaa
      961 aagatcaagc agcgcaagga cttgcgagac tattgaggag ggatcttttc ttgacaaatg
     1021 caagcgaggt cttaagctcg atccagtccg cctttcctgg ggcggtatca gacgccagag
     1081 tggagtcgga taacaggtta tgggccacaa tcgactccaa gaagatccgg gatgtatgca
     1141 aatacatgac cgataagctc ggtttcgacc actatgcagg ttcagcaggg gtcgactgga
     1201 tcgccagaaa tgagatggag gtcgtcgaga tcatggccag ctatggagtc cataaggtgg
     1261 tggccatgct gaaggttaaa acgccacgcg acaatccatc cgtcccctcc atggtcggca
     1321 tgtactggaa tgccaactgg tatgagagag agatctggga gatgttcggc atcaacttcg
     1381 atgggcaccc ggagctatat cctcttcttc tgtccgatcc attagttggg tcctggccct
     1441 ggagaaagga tttcaagggc tatcctgacc tgaccactgg agagagggca gtgcagatca
     1501 ccactcctga gggctatacc gaataccact tccccccaac accggcagag gtcgaggcgg
     1561 ggacggtggt gacagagaga cccaaatatc ccacattccg ggagagggag gaagcccaga
     1621 tcaaatccga ctcggagatg atcatgcatc tggggccgca gcatgccatg gttcccgggc
     1681 catttctgct cgatatactg gttgaaggag agagggttaa gaaggcattt ctcgatatcg
     1741 gctacatcca caaaggcatc gagaagatca tggagaacag aacctggctc caggggataa
     1801 cctataccga caggatgtgc tacgtcgctt ctttaactaa taatgagtgc tattgcggag
     1861 cggtagagag gcttttgggc ctggaggtac ccctaagggc tcaatacatc cgggtcatct
     1921 tagaggagct ttccagaatc cagagccacc tgataggcac tggcgagttt ttgaccctga
     1981 tcgctggggt gggctttgcc ccctggcagt acatgatcat ggatagagag aagatcatct
     2041 ctctgattga gagcgtcacc ggagccagac ttactcattc attcgtccgt tttggagggg
     2101 taagaaacga ccttccagag ggctttgacg agcactgccg aaaggtcttt ccctacatga
     2161 agtccaggat cgaggagttc attgagctct tcgctcagga tcccatctac catgccagga
     2221 tggagaatat cggctccata tcgcccgaga ccgctaagcg aattggctgc gctggaaggg
     2281 tgctgagggc ggctggcgtt ccttatgaca tgagaattga ggaccccatc ctggtctatc
     2341 cggagcttga cttcaaggtg gtcactggaa cccgtggcga ttcggcggac agaatcgatt
     2401 gcactctaag ggagatgctg gagagcattc atatcataga gcaatgtctg gacagaattc
     2461 cctccggacc gataaagacc gaggccaaga ttcccaagaa gattcccgca ggcgaggcct
     2521 actatcgggt cgaggacccg cgcggcgaga tgggaatgta tgtcatcagc gacggaggcg
     2581 acaagccata ccgagtgaag gtgagagggc ctttctatgc tacattccag actttgactc
     2641 cgcttcttga aggggtgtac attgcagatg cggtagcaat tgccggaagc atggacggct
     2701 gcccatctga atctgacagg taggagggag aggatttgga cggaataata gataccatca
     2761 ctcaatttgc tgcccaggag ccggcaatat tcgccctgat cataggcata atcggcgcag
     2821 cggtgctttc tgccgtggtc aatgtaggcg caatgctggt gatctgggca gacagaaagg
     2881 taatgagcga tttctgcaac cggttcggcc ccaaccgggt gggtggcaga tggggtatac
     2941 ttcagctggg cgctgatgcc atcaagctat tcaccaaaga ggatgtcata cctgcaggag
     3001 tcgataaagg ggtctatgtc tgggctccta tcatcgcctc tgccggcacc atgctggtgg
     3061 ctgcagccat accatttgga gcactgcata taggtggcaa aaactacccc ttggtagtcg
     3121 ccaatatgga tatcagcgct ttttatgtgg aagcagctct tagcatcatg tccattgccg
     3181 tcttcatggc cgggtacagc tcgaacaata agtactcaat cctcggagcc ttccgaggca
     3241 ttgccaggat gatcgcctat gaggttccaa tgggcgtttg cgtcatatcc gtggctatca
     3301 tggcccatag cctcaacctg gtggagatcg tagaaagcca gaccgtctgg tatgcatttg
     3361 cccagccgct gggatttgtc atcttcatga tcgccctggt gactgacctg ggaagagtcc
     3421 cattcgacca gagcgaggca gaagaggaga tcatctgcgg ctatagcacc gaatacagcg
     3481 gcatcaggtg gggtctctta tacttccagg agtacaataa cttcctcttg ggatccattc
     3541 tggtctcgct cctgttcctt ggcggatggc atggaccgat catacccata ccccttctgg
     3601 acatcgtatc gcctttgatc tggctgctca tgaaggttat aatcgttatt tggttcataa
     3661 tattgatcag ggctgccctc ttccgtttga gaatagatca ggtgactgac ctgggatgga
     3721 agtggatgct gcctttatcc atattgaatc tggggtgggc agtaatagtg ggctcatact
     3781 ttgcatgagg tggctgtaag atgttgaaga gaaagatgat aaagaacttc acctggcctt
     3841 tgaagaccgc ctcgcgggag atagaagtca ccaggctcta tcccgagttc atgatggagc
     3901 tgcctgatgc agagagaggg attcatgagc tggatgccac catatgcatt ggctgcggct
     3961 catgtgctag agtctgtccc aacagctgca ttgagatggt gcacttcaag ttcggaaatc
     4021 ccctcaagaa caagaagatg cagtttccgc aaatcgatta cggaagatgt atgttctgcg
     4081 gcctatgcgt ggatgagtgc ccggtcgaat gcctcaagat gggaaagaag gtcatcatgg
     4141 ctggctggga aagaaaggac atcgtgaaag gccctgattt cctggccaca aagaggttct
     4201 cagccaaaga ggcagcagac ctggaggctg aggcaaagag gattgctgct gagaaggccg
     4261 ctgccaagaa ggctgcggca aaagatgctt cagccgcggg cgacaagaag ccagccaaag
     4321 agggagcaaa tgcagaaaag aagaagaaga atgctgaggc caaacccgca gaaggaggtg
     4381 cttcctgatg tcggaaaaag gacctgaaaa aaatgtgatt agaagcatat tcgagctgct
     4441 ggtgcttttg cttgccctcg gagtcatctt cggcggactg gcggtgatag tattactatc
     4501 cccctggtct aaagcgatat tggacaggct gctcgactac gacatccgct ttgccataga
     4561 gcttttggca ttcctggcca tagcgacaat tattgtcctt ttatcggcgc tgacggtcct
     4621 ggtcaagaat attgtgcata gcgctcttta cctgctggga acctttgctg gtgtggctgc
     4681 gctctacata ttcatgaatg cccccttcgt gggtgtggct cagatcctgg tttacattgg
     4741 tgcagtggga gttctgatac tcttcgctgt gatgctcacc aggaggacga ttatggagga
     4801 gtctcatggc gaaatttaag ctcattatac tgaccctggc cttccttgct gccctggtgg
     4861 cctcgctttc ggtaaccgat tggggaccgg tcacagagca cccgctcggc tacgcccctt
     4921 cggaggggat gaggcttcct gagagcggtg ttggagacat agggtttgag atcttcacta
     4981 cctatgtctt ctcctttgag gttctggccc tggtcctgac cgcagccctg gtcggagcga
     5041 tctgggtggc aagaaaggag gacgcctagg gagggcgaca aagaatgaca aggagggaag
     5101 tttaccgatg ataccgttgg agctttacat attgctggca tccatcatgt tcaccatcgg
     5161 cttgtacggc ctcgtcacac agaagaacgg agtcaagctg atgatgtgca tcgagctgct
     5221 gctcaatagc gccaacatca atcttgttgc cttctcagcc taccagccta atatgaacgg
     5281 gcaggtattc gccctgttct ccatagcgct ggcggcagct gaagcgggcg ttggatttgc
     5341 tatattgatc gcactataca ggctatacgg aaccattgac ctggataaca tcaacaccat
     5401 gaggtggtag tctttgtatt ccgatttagc ttacctgatt gttgggctgc ctgtactggc
     5461 atttgtgctg tgcctattca tagggtcgcg cctgcctaga gggggcgggt tcctcaccgt
     5521 tttggcaact ttaggcggcc tgatcatatc tctgggaata ttccaggaga tctatccacg
     5581 cggaattatc catcagtcga tgcgctggtt tggtgatttc aatgtgggca tactgataga
     5641 tccactggct ctagtgatgc ttctcatggt cacatttgtg gttatgctga tccacactta
     5701 tgccaatgga tacatgaacg gtgatccggg tgctaacagg tactttgctg aagcagcact
     5761 attcactgct tccatgctgg gcctgattta tgccgataat atccttcagc tcttcatatt
     5821 ctgggagctg gtgggtctgt gctcctatct cctgatagga ttctggtacc gtaagccatc
     5881 tgcggctgct gcagccaaga aggcatttct taccactcgt gtgggcgacg tcatgttcct
     5941 ggcggccata attgttctct acaataatct ggccagcctg aacctcaccc tcaacccggg
     6001 agaatacctc ctgcagttcc cggtgattta tgccaatctc gccctaatac cgcctgatca
     6061 gttaacactc attgcccttg gctttttggg aggtgcagtg ggcaaatccg gtcagttccc
     6121 cctgcatgtc tggcttcctg atgccatgga gggtccaact accgtctccg cgatgatcca
     6181 tgcggccaca atggttaccg ctggtgtcta tctcgtagcc cgaatgttcc ccttattcta
     6241 tgctgccccc cacggcctta ctgcggtggc ggcagtagga gcattcactg cattatttgc
     6301 agcaactatg ggcctgacgg catttgacat caagagagtg ctggcattct cgaccgtctc
     6361 tcagctcggc ttcatgatgg cgggtctggg agttggcgcg gcagtaggag ctttttcagt
     6421 gggagtttct atattccacc tcataggcca ctcattcttc aaggccctgc tcttcctctg
     6481 tgccggctct gtgattcacg ccgtcaatac aaacgatatg cgtgagatgg gaggagttgg
     6541 gaagtacatg aagtggacca tgtacaccat gctcatagga tccctctccc tggccggatt
     6601 ccccctgttc acgggattct tctccaagga tgagatcata gtgattgcct atgagtacgg
     6661 tgtggcgatc aacttcctgc cttacatatt ccttgtgctg gcagcagttc tcacggccat
     6721 atacaccttc agaatgtggt ttagcgtctt cacgggcaaa gagagatcca actacggaaa
     6781 gcacgaatcg ccctgggtca tgctcgggcc gctagtgatc ctggcggtat ttgctcttgc
     6841 attcggcttt gtcgcccaag aaggcttcta taattacgtt ggggacaatt tcggtcaata
     6901 cgaggagaag tttggtgtcg atgtaaactt cgcagaattg gctcttattg gaggccatga
     6961 ggttttggga gagcacggtg ctcctgccga aggcgagcat ccgtctgccg aggccgagga
     7021 aggccacggt gccgctggcg aaggagaagc tggtggcgaa gaagctgaag gagagccatg
     7081 gtacattcag atattgccga tcttcatagc cataggcgga attctgatcg cagccctgtt
     7141 ctactgggat agagccaaga agtttgaccc cagccaggtg acaggtgaca aagatccaat
     7201 cagaaagatg ctgctcaaag gctactatca gcatgagata atgaccggct ggatatctga
     7261 aggcatagta tacggcacgg ctctgatctg caatacgatc gacatcaagg tcgtagacgg
     7321 ctggcttaac tggcttagcg cctgggtcat gggctttgca ggtcacgtga gaaaggttca
     7381 gacgggcgtg gtgcaaaact acgtcacagc tttaatgcta gggatagtgg tgctggtgat
     7441 tgcaattgct gctatcaggc tggcaatgga ggtgggcctg atatgatctc aaacgcgctc
     7501 tcgatgatga tactcattcc cttgctggga gcgatagtat ccttctttgt gggcacaaag
     7561 gccaaattcg tggctctgat cgcctcattt gcgcctctgg tcttatccct gcagatgtac
     7621 atggagttcg ataagagctc aactatgatg cagttcgtgg agagttacga ctgggttcca
     7681 tccatcggag tcaagtatac attgggcgtc gatggcatag gatttcccct ggttcttctc
     7741 tccaccatag tcaccatact ggtgataata tactcctggg gagagacaaa gaagcccaat
     7801 cagtatttcg ccctgcttct cttaaatgag gtgggagtgc tcggagtgtt cactgcactg
     7861 gacttcttcc tgttctacat cttctgggag atagtgctca tccccatgtt cttcctcatt
     7921 ggatcatggg gcggaccgag aaagga
//
LOCUS       DAOSGP010000035         7811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_8064_length_7811_cov_17.973956, whole genome shotgun sequence.
ACCESSION   DAOSGP010000035 DAOSGP010000000
VERSION     DAOSGP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8064_length_7811_cov_17.973956"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLV49_01950"
     CDS             <1..510
                     /locus_tag="PLV49_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOG97633.1"
                     /translation="ETAGLFKKMKEDSNMSQSELMRESLKFFGKHKLLFEYAEDNKVY
                     THAEMLAAGEHVILDIDHWIMFLSFIETHPEKEKFWSLHKEVSRAHAEQFKHKLFNAE
                     TILRRLEICNLFTMNKTGKNEFTLIFGSDAPKKFVLKELEEIFDGMGFLVEIKEDFSK
                     LRIKVIHDL"
     gene            550..1053
                     /locus_tag="PLV49_01955"
     CDS             550..1053
                     /locus_tag="PLV49_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97634.1"
                     /translation="MNDVSPSMDLISLAEDFIAKKQEEISLQLAGKYLLSSDEVSEIV
                     SKFLEDSYGPAMALAGEIAGREGLVLLFIGRKDCAICQRCYPVLERFIIEHREIEPVI
                     LDYSQTEGLIYHLIHREEGGMLPLIAFIYKGKIMMKTCGECAAEHVYEKCYRDIRDDC
                     SQNLYVH"
     gene            1081..1746
                     /gene="tpiA"
                     /locus_tag="PLV49_01960"
     CDS             1081..1746
                     /gene="tpiA"
                     /locus_tag="PLV49_01960"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOG97635.1"
                     /translation="MTIIVLNFKTYRESTGSEALRLAEICEDIARDYSVQMIVAPQTA
                     DIHAISREVKIPVYAQHVDGVGYGGFTGHVTAASIKAAGARGSLINHSERRIKLADME
                     ASLSACRDFALASIICTNNVATTRAAAALEPDYVAVEPPELIGSGIPVSKANPSVVSD
                     SVSAVKKIAPRVGVLCGAGITHGEDLRSALDLGSEGVLLASGIIKAKDQRQALEDLVT
                     GAK"
     gene            1750..2016
                     /locus_tag="PLV49_01965"
     CDS             1750..2016
                     /locus_tag="PLV49_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOG97636.1"
                     /translation="MSVHVRISGRVQGVYYRAYTRDRAKSLGINGWVRNIPGGGVEAV
                     LEGERRQVGELLRAMKSGPSGSVVLGMELSEIEAKGYNDFEIKY"
     gene            2032..2202
                     /locus_tag="PLV49_01970"
     CDS             2032..2202
                     /locus_tag="PLV49_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG97637.1"
                     /translation="MLTVNRYKCCYCGACVSVCPTCALELVETWLEISAECKECGICT
                     KICPVGALEVEA"
     gene            2199..>2689
                     /locus_tag="PLV49_01975"
     CDS             2199..>2689
                     /locus_tag="PLV49_01975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG97638.1"
                     /translation="MKCDVIVVGAGPGGSMAAKTAAAAGLNVVMLEKRQEIGDPVRCA
                     EGVGKRALCKMVKPEPEWIASEVKGAKIYSPDGSSIVMSEDRSGSEVGYVLERKVFDR
                     ALAMQAARAGARVLVKTRAASLLMNDGVPFGVNAIQVGDPLKIEAPIIIGADGVESKV
                     GRWA"
     assembly_gap    2690..2789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2790..3142
                     /locus_tag="PLV49_01980"
     CDS             <2790..3142
                     /locus_tag="PLV49_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG97639.1"
                     /translation="GDAARQTDPLTGGGILNAMEAGVIAGEVAAKAIAAGNVAREGLR
                     EYEDRWREGIGRHLARSLEFKEFFVKLTDKDLNMLIGSLAKEDISKLDLPGLLRVLFR
                     LNPKLLWELRHLVT"
     gene            complement(3186..3734)
                     /locus_tag="PLV49_01985"
     CDS             complement(3186..3734)
                     /locus_tag="PLV49_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97640.1"
                     /translation="MIAIEIDKRDLDELTKTEVKNLPGALFAGASPLLKPFMKKLEAL
                     LPQENKGRGDSYVLCALHSHIDEVHADESQIVVKSSDEIVEIRREELAELMDERYPTT
                     GHQRLNLPGLLFLQSGPALQSASAMILRREHKLRIPDGRRTMRYIFHMGVVKLDADKE
                     KIKIGFDLERLPKKADGTSTLE"
     gene            4111..4488
                     /locus_tag="PLV49_01990"
     CDS             4111..4488
                     /locus_tag="PLV49_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOG97641.1"
                     /translation="MRESTVKILDDKDMEFVETLRSLGVPRNVATLITFLANVDEASS
                     REIEMGSDLRQPEVSIAMRTLRDNNWIEEKEIKREGKGRPMKVYSLKAGIEEVIKHFE
                     EEKLHESAQAMESIQRLKQLIST"
     gene            complement(4531..4791)
                     /locus_tag="PLV49_01995"
     CDS             complement(4531..4791)
                     /locus_tag="PLV49_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOG97642.1"
                     /translation="MRYIELSRAPCNQTLEIHSVNGEYGWERRFESMGIRRGSRVRKI
                     TCHPFGGPVVIELNGSKISLGRGIAAKIEVEVLSSPSVKKQI"
     gene            complement(4889..5701)
                     /gene="hisF"
                     /locus_tag="PLV49_02000"
     CDS             complement(4889..5701)
                     /gene="hisF"
                     /locus_tag="PLV49_02000"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOG97643.1"
                     /translation="MLARRIIPCLDCDLGVPGGRVVKGIEFKQIRYAGVPWELADQYY
                     QDGADEIVFLDITASSDRRETMTSVIKKTSLSVFVPLAVGGGISSLAQSREVFNAGAD
                     KVTVNTSALKRPDLISEIAGRYGNQAVVLAIDAKRRYSQEDGRNMVQTEEGPCWFECS
                     YYGGREFTGIDAIAWAKKAVSLGAGEILLTSMDRDGTKLGFDIPLTAAVARMVRVPVI
                     ASGGCASPEHMLEVFQKTDASAALAASIFHFQECSIIDVKTYLRENGVNVRL"
     gene            complement(5695..6300)
                     /gene="hisH"
                     /locus_tag="PLV49_02005"
     CDS             complement(5695..6300)
                     /gene="hisH"
                     /locus_tag="PLV49_02005"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOG97644.1"
                     /translation="MRIIIVDYGMGNLFSIYNALEYVGGNPKIARDPGELAGAKGIVV
                     PGVGAFGSCMNQLSRFSDSLLSCFQEGVPMLGICVGMQVLFQESEESPGAPGLGWIEG
                     KVVRLPDGVMIPQMGWNSLSIKHRVEIFDGINEGDMFYFVHSYYGQPRDNSVIAATTD
                     HGVELAAAVHKDNLFATQFHPEKSGPKGLRILKNFVRSTIC"
     gene            6724..>7811
                     /locus_tag="PLV49_02010"
     CDS             6724..>7811
                     /locus_tag="PLV49_02010"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOG97645.1"
                     /translation="MTKSNPFEIAQKQLDDCAKILNLDPGVHEFLRHPRREFHVSIPV
                     RMDDGSIKIFQGYRVQYNDALGPCKGGIRYHSDETIDTVRALAAWMTWKTSLLGLPLG
                     GGKGGVICNPKELSTGEQERLCRGYVDMVWQNIGPERDVPAPDVYTTPQMMAWMMDEY
                     SKLTGSNNFGCITGKPLCVGGSCGRSDATARGGMYALREAARELGIDLSRATIAIQGY
                     GNAGSYAHSLAKELFGSKVVAVSDSKGGAFNPAGIEPAEASSVKAETCTVASIPDAKR
                     ISNEELLELNVDILIVAALENVITKENAGNIKARIILELANGPTTPEADEILFNNKVH
                     VIPDFLANAGGVTVSYFEMVQNIMRYCWS"
BASE COUNT         1897 a         1971 c         2048 g         1795 t
ORIGIN      
        1 gagacagctg gcctgttcaa gaagatgaaa gaggactcga acatgtccca gagcgagctg
       61 atgcgagagt ctctgaagtt ctttggcaaa cataagttgc tctttgaata tgcggaggac
      121 aacaaggtct acacccatgc agagatgctc gctgccgggg agcatgtcat acttgatatc
      181 gaccactgga taatgtttct gagcttcatc gagacgcatc cggagaaaga gaagttctgg
      241 agcctgcata aagaggtcag ccgggcccat gccgagcagt tcaagcacaa gctcttcaat
      301 gccgagacca tcctccgccg gctggagatc tgcaacctct ttaccatgaa taagaccggc
      361 aagaacgagt tcaccctcat cttcggatcg gatgctccca agaagttcgt ccttaaggag
      421 ctggaggaga tcttcgatgg catgggcttt ttggtggaga tcaaagagga cttctccaag
      481 ctcaggatca aagtaattca tgatctataa gaaaaattaa tcccctagaa ggatcatacc
      541 cccgaaaaga tgaatgacgt ttccccttcc atggatctca tctccctggc agaggacttc
      601 atcgccaaaa agcaggaaga gataagcctg cagctggcgg gaaaatacct gctatcctca
      661 gacgaggtat cggagatcgt atccaaattt ttggaggatt cttatgggcc ggccatggct
      721 ctggcagggg aaattgcagg gagagagggg ctggtgctgc tatttatcgg caggaaggac
      781 tgcgccatct gccagcgctg ctatccggtc ctggagagat tcatcattga gcacagggaa
      841 atagagccgg tgatcctcga ctactcccag actgagggac tgatctatca cctgatccat
      901 agagaagagg ggggaatgct gcctttaatt gctttcatct ataaagggaa gataatgatg
      961 aagacctgtg gcgaatgcgc tgcagagcat gtttatgaga aatgctatcg cgacataaga
     1021 gacgattgca gccaaaattt atatgtccac tgagatcgca ccggagagcc gaggaagaat
     1081 atgactataa ttgtgctcaa cttcaagacc taccgtgagt caacaggctc ggaggctctg
     1141 cgcctggccg agatctgcga ggatatcgcc agggactatt ctgtgcagat gatagtggca
     1201 ccccagacgg cagacatcca tgccatatct cgtgaggtaa aaattccggt ctacgcccag
     1261 catgtagatg gcgttggcta tggcggattt accggccatg tgaccgccgc ttccataaaa
     1321 gcagcaggtg cgagaggttc actcataaac cactcggaga gaaggatcaa gctggccgat
     1381 atggaagcct ccctctccgc ctgccgggat tttgccctcg cgagcatcat ctgcaccaac
     1441 aatgtggcca ccaccagggc tgcggcagct cttgaacctg attatgtggc agtggagcct
     1501 cctgaattga taggaagcgg aattccggta tccaaggcca atcccagcgt ggttagtgat
     1561 tcggtcagcg cagtcaagaa gatcgcccct cgtgtggggg tcctctgcgg cgcggggata
     1621 acccacggcg aggatctgag aagcgctctg gacctgggat cggagggcgt cttgctggca
     1681 tcgggaatca ttaaagccaa ggatcagcgt caggctttag aggatcttgt gactggggca
     1741 aaatagatga tgtcggtgca cgtcaggatt tctggccggg tgcagggtgt ttattatcgc
     1801 gcctataccc gtgaccgagc caaatccctg ggaatcaatg gatgggtgcg aaatattccc
     1861 gggggcggag tggaggcggt gctggaggga gagagacgac aggtggggga gctgctccga
     1921 gccatgaagt ccgggccttc cggttctgtg gtcctgggta tggagctctc ggagatcgag
     1981 gccaaaggct ataatgactt tgaaataaaa tattaaaatg gatgtgattt attgcttaca
     2041 gtcaacagat acaagtgctg ctactgtggt gcctgcgtca gcgtatgccc gacatgtgcc
     2101 ctggagctgg tggagacctg gctggagatt tccgcagagt gcaaagagtg cggcatctgc
     2161 accaagatat gtccggtggg agcgctagag gtcgaggcat gaagtgcgat gtgatagtgg
     2221 ttggagcggg cccaggcggc tccatggccg cgaaaacggc agcagcagcc ggcctgaatg
     2281 tggtcatgct ggagaagcgt caggagatcg gcgatcctgt tcgctgcgct gagggggtgg
     2341 gcaagagggc cttatgcaag atggtcaagc cagagccgga gtggatcgcc tctgaggtaa
     2401 aaggcgccaa gatctactct ccagacggaa gcagcatagt catgtccgag gaccggagcg
     2461 ggagcgaggt tggctacgtt ctggagagaa aggtcttcga ccgggctctg gctatgcaag
     2521 ccgcccgcgc cggggcccga gtgctggtca agactcgcgc tgccagcctt ctaatgaatg
     2581 atggcgttcc ttttggcgtt aatgccattc aagtgggaga tcctctgaaa atcgaagccc
     2641 cgatcatcat cggtgcggat ggcgtagagt caaaggtggg ccgctgggcn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cggagatgcc gcccgccaga ccgatccact
     2821 gactggaggg gggattctca atgccatgga ggcaggagtc atcgccggag aggtagcagc
     2881 aaaagcaatt gctgcaggca acgtcgcgag agaggggctt cgagagtatg aggaccgctg
     2941 gcgagagggc ataggcaggc acctggctcg cagcctggag ttcaaggagt tcttcgtaaa
     3001 gctgaccgac aaagatctca atatgctgat cggttccctg gcaaaggagg acatatccaa
     3061 attggatctg cctggcctct tgcgggtgct cttccggttg aatcccaagc ttctctggga
     3121 gttgcgccac ctcgtaacat agttgccggt cttcaaagga tctggattct gaatatggct
     3181 ttggcctatt ccagggtgga ggtgccatct gccttctttg gcagcctctc caggtcaaag
     3241 ccgattttta tcttctcttt atccgcatcg agcttcacca cgcccatatg gaagatgtag
     3301 cgcatggtcc ggcggccatc ggggatgcgc aatttatgct cccggcggag gatcatggcg
     3361 ctggcggact gcagtgccgg gccgctctgc aaaaacagca gtccgggtag gttgagccgc
     3421 tggtggccgg tggtcggata tctctcatcc atcagctctg ccagttcttc gcgacggatc
     3481 tcgacgattt catcgctgct tttgacaacg atctgactct cgtcggcatg cacctcatct
     3541 atatgagaat gaagggcaca gagcacatag ctatcccccc ggcccttatt ctcctggggc
     3601 aaaagagctt ccagcttttt cataaagggc ttaagaagag gactggcacc ggcaaaaagt
     3661 gcccccggta ggtttttaac ctcagtcttt gtcagctcat ccagatccct tttgtcaatc
     3721 tcgatggcta tcatagagaa ctgaaggacc ttacgagata tatctatatc gggacgaata
     3781 aattgcgatt ataaaaaaat tttcaaactt atactaaatt attctctata aattaattat
     3841 ttaactctat tttgtagttg ctttcgcaag aatggcaaaa aaatggcttt aatttttgag
     3901 gaatatcatc ccctcaaaaa atcatcgtga ttataaattc ctaatcaggc ttattttatc
     3961 ctaaataacc atctaaatgc gaatatcagc tgcattttaa cttattatct aaagtattag
     4021 tctaaataag taaaattaaa agaccaagcc tttcaaaaca aattatttat atcattaagt
     4081 attagtggcc agaaaaatat ggtgacgatt atgagagagt cgacagtaaa gatcctggat
     4141 gacaaggaca tggaattcgt ggagacgctc cgctcccttg gagtgccccg aaacgtagca
     4201 acccttatca catttctggc aaatgtggat gaggcctcct caagggagat agagatgggc
     4261 tcagacctca ggcagccaga ggtgagcatt gccatgagaa ccctgcgcga caacaactgg
     4321 atagaggaga aggagattaa acgcgagggc aaaggaagac cgatgaaggt ctactcattg
     4381 aaagcgggca ttgaagaggt cataaaacat ttcgaggaag agaagctgca tgagtccgct
     4441 caggccatgg agagcatcca gaggctcaag cagctcatat caacataaat tatccaggat
     4501 caattattgc tcgatcgatg gatgaggctt tcagatctgt ttcttaactg atggagatga
     4561 aagcacctca acctctattt ttgctgctat tccccgtcca agcgagatct tagatccgtt
     4621 aagctcgatt accaccggtc cgccaaaggg atggcaggtt attttgcgca cacggctacc
     4681 tcttcttatt cccatggatt caaacctcct ctcccagccg tactcaccgt tcacggaatg
     4741 aatctccagg gtctgattgc agggtgccct tgagagctct atatacctca tctccgatca
     4801 tccacctcgt cgccatcatc tccagaacac ggataaatct tgcgcagcga tcatcctttc
     4861 gcaatccttc tcttcccaat caattgcctc ataatcgcac attcacccca ttctccctca
     4921 gatatgtctt gacatcgatg atgctgcatt cctggaagtg gaagatgctg gccgccaggg
     4981 cggcagaggc atcggtcttt tggaagacct ccagcatatg ctcaggactt gcgcatcctc
     5041 ctgaggcaat cactggcacc cggaccattc tcgccacagc agcggtgagg gggatgtcaa
     5101 acccaagctt ggtgccgtct ctgtccatgc tggtgagaag gatctctcct gctcccaggg
     5161 agacagcctt tttggcccag gcgatggcgt caattccggt gaactcccta ccgccgtaat
     5221 aggagcattc aaaccagcag ggcccctctt ccgtctgtac catattccgg ccatcctcct
     5281 gggaatagcg ccttttggca tctatggcca gaaccacggc ctgatttccg tatctaccgg
     5341 cgatctcgct gatgagatcc ggtcgcttta gggcggaggt gttaacggtg accttatccg
     5401 caccagcgtt gaagacctcc cttgattgcg ccaggctgct tatccccccg cccacggcca
     5461 ggggcacaaa aaccgagagc gatgtcttct ttatcacact ggtcatggtc tcccgccggt
     5521 cactggatgc agttatatcc aaaaagacga tctcatccgc accatcctga tagtactgat
     5581 cggccagctc ccaggggact ccggcatatc tgatctgctt gaactcaatt cccttgacca
     5641 ctctgcctcc ggggaccccc aggtcgcagt ccaggcaggg aatgatcctt ctggctagca
     5701 tatggtggac ctcacgaagt tcttcaggat tctaaggccc tttggccctg atttctcggg
     5761 atgaaactgg gtggcaaaga ggttatcctt atggactgca gctgccaatt ccactccgtg
     5821 atctgtggtg gcggctataa ccgagttgtc ccttggctgg ccgtagtagg aatggacgaa
     5881 gtagaacatg tccccctcat tgatgccatc aaaaatctcc acccggtgct tgatggagag
     5941 gctgttccag cccatctggg gaatcatcac tccatccggc aggcgcacaa ccttcccctc
     6001 tatccagccc aggcccggcg cacccggact ctcctcgctc tcctggaaga gcacctgcat
     6061 gccaacgcat atgcccagca tcggcactcc ctcttggaag catgatagga gcgagtctga
     6121 gaatcgagag agctgattca tgcagctgcc aaaggccccc acacccggga caactatgcc
     6181 tttcgccccg gccaactccc ctggatcgcg ggcaatctta ggattgccgc ccacatattc
     6241 cagggcgttg tatatggaaa ataggtttcc cattccataa tccactatga ttatcctcat
     6301 ctgcgtcttc cttctcgcta tggcgcgatt ttatttggcg ccattaatat ctctatttgc
     6361 gatcctttca tatcttttca taagcctgtt ggctcggcgg aacatatatt cctgcgaggc
     6421 accatatccc gcagggcata aggattatga actttactaa taaatttttt aagattattt
     6481 atgatttctt cacaatttcg cattcataga gcttcatctt gactcaattc gtccgttgtt
     6541 tttccatttc ttgtctgcaa tttaattata gcaaatcttt aaataggccg acaactcaat
     6601 tccgcccttc tccatcactt aaaaacttga cccataatac ttaaataggg aacgatggac
     6661 acctccctcc accaggtcat aaaaattaag attcgggaaa atacttaatg gagatgcaat
     6721 ataatgacca aatcgaatcc ttttgagata gctcaaaaac agcttgatga ttgtgccaag
     6781 atcctaaacc tagatcctgg tgtgcatgag ttcctccgcc accccaggcg agagttccac
     6841 gtatcgattc ccgtgcgcat ggatgacggc agcataaaga tcttccaggg atacagggtt
     6901 cagtataacg atgctctggg cccctgcaag ggcggcataa gataccattc ggatgagacc
     6961 atcgataccg tgagggctct agccgcctgg atgacctgga agacttccct tctcggcctg
     7021 cccctgggcg gaggtaaagg tggagtgatc tgcaatccca aggagctctc caccggagag
     7081 caggagaggc tctgccgggg ctatgtggat atggtctggc agaacatcgg accggagagg
     7141 gacgttcctg cgccggacgt ctacaccact ccccagatga tggcctggat gatggatgag
     7201 tactcaaaat tgaccggctc gaacaacttc ggttgcatca ccggcaagcc tctgtgcgtg
     7261 ggcggttcct gtggcagaag cgatgccact gcccggggag gcatgtatgc tctgagggag
     7321 gcggcaaggg aattaggaat tgatctctcc cgggccacaa ttgccattca agggtacggc
     7381 aatgccggca gctatgctca cagcctggcc aaggagcttt tcggcagcaa ggttgtcgcg
     7441 gttagcgact ccaagggcgg agcattcaat ccggcaggaa tagagccggc agaagcatca
     7501 agcgtcaagg cggagacctg taccgtggcc agcatacccg atgccaagag gataagcaat
     7561 gaggagctcc tggagcttaa tgtggacatt ctcattgtcg ctgctctgga gaacgtgatc
     7621 accaaggaga atgctggaaa tatcaaggcc aggatcattc tggagctggc caatggtccc
     7681 accacacctg aggcagatga gatcctcttc aataacaagg tgcatgtcat ccccgacttc
     7741 ctggccaatg ccggcggagt caccgtctcc tactttgaga tggtgcagaa catcatgcgc
     7801 tactgctggt c
//
LOCUS       DAOSGP010000036         7592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_8358_length_7592_cov_18.811198, whole genome shotgun sequence.
ACCESSION   DAOSGP010000036 DAOSGP010000000
VERSION     DAOSGP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8358_length_7592_cov_18.811198"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLV49_02015"
     CDS             <1..645
                     /locus_tag="PLV49_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG97646.1"
                     /translation="IMSNMLDPIELLDILGNENRRRILQLLSFRPFYFNEMAKRLDVG
                     PKAVIDHLEMLERAGLVECYQEQGRRKYFRIARRTVLEIAVSPHSYGVRAYLSEDAPK
                     DASGFEEAEASLDLKMLKDELLGLEKKRHELRELLAQIESREMEIKQRASCVAGFRAE
                     NQLESEIMTALLSGGANSSQLATRLEVPEAVVLDSLNKLKERSAVHLRGQEWSI"
     gene            753..2930
                     /locus_tag="PLV49_02020"
     CDS             753..2930
                     /locus_tag="PLV49_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HOG97647.1"
                     /translation="MKPAEDASVLLRVAEAYHKDAGKGVARINPIILAQLGVENGGVV
                     EINARDKVYAIAWPGTPEDPQDIIRIDGNTRSNLGTGIDNRVNVRRATARPARKIVVA
                     PTRQIRLMGGQQYLLRMLQGRAVVKGEMLRVEMINSSLNLAVVSTVPNGPVLVTQETI
                     ISITRETLDELALHVRDISYEDIGGLSREIREIREMIEVPLRHPELFSRLGINPPRGV
                     LLHGPPGTGKTLIARAVAGETDANFISISGPEIVSKFYGESEQRLRQIFDEASKAAPS
                     IIFIDEIDSIAPKREEVSGDLERRVVAQILSLMDGLSSRGEVIVIAATNRPNALDPAI
                     RRGGRFDREIEIGIPNRNGRLEVLYVHTRGMPLDESLDLMEIADSTHGFVGADLYALC
                     KEAAMRTLERALPDLDVKEDIPLDVLDNLNVTREDFLSALKKIEPSAMREVFVEVAQV
                     HWDEVGGLDEAKRSLVEAVEWPLMYPEAFASVGVRPPRGILLYGLPGTGKTLLVRALA
                     TESNVNFISVKGPELLSKWVGESERAVREIFRKARQAAPALVFFDEIDSIVPARGSGS
                     DSHVTERVVSQFLTEMDGLMELKDVVIVAATNRPDLLDSSLLRPGRFDRLVYIPMPDK
                     EARQKILEIYLSKMPAYEVSAQWLADITENFSGADLEMLCREAGMLALREHIRPGMKR
                     EELIVDKILVTEKHFQEASEYIRPHLSKDMLQGYTKMIREFQV"
     gene            3022..4926
                     /gene="lonB"
                     /locus_tag="PLV49_02025"
     CDS             3022..4926
                     /gene="lonB"
                     /locus_tag="PLV49_02025"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOG97648.1"
                     /translation="MTSEDESNELLGDIKFEDTSSITVPENLIDQVIGQDEAVEVIKK
                     AASQRRHVMLIGSPGTGKSMLGKAMSELLPVEDLQDVLVYANPEDNNTPRVRVVPAGR
                     GKQIVDAQKLEARKKVQTRNMFLMIIVMALIVYAYYMGQLLFGIIAAALLFISLRYML
                     PKEDAMVPKLLVDNNGKKKAPYVDATGAHAGALLGDVRHDPFQSGGLETPSHERVECG
                     SIHKAHKGVLFIDEVNTLRPESQQSLLTALQEGVYQITGQSERSSGALVRTEPVPCSF
                     IMIAAGNLDAVQGMHPALRSRIKGYGYEVYMRDTMEDTLENRNKLIRFVAQEIVRDGK
                     IPHFTQDAVAEIMREAKRRSGRKGHLTLMLRDLGGLIRVSGDVARAESSHLTEVNHVM
                     QAKKMARSVEQQLADRYMERRKDYSMFHSSGDEIGRVNGLAVMGDSGIVLPIMAEITP
                     AQSKEEGKIIATGKLQEIAKEAVTNVSVLIKKFLGEDITKKDVHIQFIGTYEGVEGDS
                     ASISIATAVISALEGVPVKQTVAMTGSLSVRGDVMPVGGVTQKIEAAAQAGIKTVLIP
                     KSNMGDVLIDDSMKNSIEIIPVSNISEVFEYAMGSQRTRLIEKLKKFATEKKIGINIP
                     ETIPTPIRSI"
     gene            4931..6250
                     /locus_tag="PLV49_02030"
     CDS             4931..6250
                     /locus_tag="PLV49_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOG97649.1"
                     /translation="MTAEFHDTRILQGSRTRIVLDNGKLEEIAQVPFQGASVRALFGG
                     AWGFVTTDRVDGLGQEIDLAKRIARKIGRKEDLSLAEAPPGRSVVVPVKRDPKDLSLE
                     EKVALLREIEDAAKVKGISSTQAVYSEMDLVAHYSSSEGLDLESRMTRMGFVISAVAH
                     RNGLYQTDGEGRAGVGGLELFDREDPIALARQVGETAVALLDAKAARGGTHPVVLDQE
                     LAGVFVHEAVGHATEGDIILEGDSCLEGKLGQRIGSELVTVKDDPSLMLNGYYPFDDE
                     GSRAQETVLVENGVLRSYLNTRETAARLGGVPRNARAEGISRPVVRMSNTYIANGDWK
                     LEEILEELKSGVYLAGSRGGQVSTGEGIFQFNAKKGYIVENGEKTQLLRDVSLSGKIL
                     ETLLRVLAVGDDLRYNSGRCGKSGQLVPVSDGSPHLLVDRATVGGTG"
     gene            complement(6289..7053)
                     /locus_tag="PLV49_02035"
     CDS             complement(6289..7053)
                     /locus_tag="PLV49_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOG97650.1"
                     /translation="MNISYLLRLFVLILTIFSVLSLLPGSSASPDEVYGVVTNVIDGD
                     TFDLRIEKADSRISDSVEQITLADVRSPDMKAIQGPAARDFTYAVLMDKRVYLDVDDF
                     NRGRDDEGRLIAVAYLAGAYGQPIPYPNFNLLLVDAGHAILANQTDNEFDPKNWREEH
                     NSESGSSDTLQRVEENLRERLEESLQGSGQEQKGTQNDGWQNDSQNGLLSRIEDAAGE
                     KLNRATQAAWDWLKAEITNTSLEMVNSSLDRALNRS"
     gene            complement(7083..7451)
                     /locus_tag="PLV49_02040"
     CDS             complement(7083..7451)
                     /locus_tag="PLV49_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97651.1"
                     /translation="MAQKKTYWEMQKSFWKTPQGVVIWTAALLAIMAGGILALNFLGS
                     PYPVIEFFDAEPEFLAPGQSSVLSWSVVGASLVEIDQDIGPVALEGSRSVSPSEDTIY
                     RLVAVNGSRNRSAELKVSVS"
BASE COUNT         1782 a         1950 c         2281 g         1579 t
ORIGIN      
        1 attatgagca acatgttgga tcctattgag cttcttgaca ttctggggaa tgaaaataga
       61 agacgtatcc tccagctcct ctctttccgc cccttttact tcaatgagat ggccaaacgg
      121 ctggatgtag ggccaaaagc ggtgatcgac cacctggaga tgctggagag agcggggctg
      181 gtggagtgct atcaagagca gggacggaga aaatacttcc gcattgccag gagaaccgtt
      241 ctggagatag cagtctctcc tcattcatat ggggtccggg catatctttc agaagatgct
      301 ccaaaggacg cctcgggctt tgaggaggct gaggcctctt tggatctgaa gatgctcaaa
      361 gatgagctct tgggcctgga gaaaaaaagg catgagctgc gcgaactatt ggcccagatc
      421 gaatcgcgag agatggagat caagcagaga gcatcctgcg ttgccggatt tcgggcagag
      481 aaccagcttg agtcggagat catgactgca ttgctcagcg gcggcgcaaa ttcatcccag
      541 ctcgcgacca ggctggaggt tccagaagcg gtggttttag atagcttgaa taagctcaaa
      601 gagcgcagtg cagtgcactt aagaggtcag gaatggagca tatgagaata tcctcaagca
      661 acccgtcatc atcagcgtcc tcgccatctg cagacaatcc ctctccaggg gatgaatcca
      721 gccccagcat cgaggagatc gtccagggag gcatgaagcc agcagaggat gcatcagttt
      781 tgcttcgagt ggctgaagca tatcataagg atgcggggaa gggcgtggcc aggatcaatc
      841 ccatcattct cgcccaattg ggagtggaaa acggcggggt ggtggagatc aatgcccggg
      901 ataaggtcta tgccatcgcc tggcctggaa ctcctgagga cccccaggat ataatcagga
      961 tcgacggaaa cacccggtcc aacctgggga cgggaataga caaccgggtg aatgtccgcc
     1021 gggccacggc caggcccgcc agaaagatcg tggtcgcccc cacccgccag atccgcctca
     1081 tgggaggaca gcagtatctc ttgcgcatgc tccaggggag ggcggtggtt aagggggaga
     1141 tgctgcgggt ggagatgata aacagcagcc tcaacctggc agtggtgagc accgtgccaa
     1201 acggcccggt tctggtaact caggagacga tcatcagcat caccagagag accctggatg
     1261 agctggccct gcatgtgaga gacatttcct atgaggacat cggcggtctg tcccgggaga
     1321 tcagggagat cagagagatg atcgaggttc cgctgcgcca tccagagctc ttcagccggc
     1381 tgggaatcaa tcctccccgt ggggtgctcc tgcacggtcc tccgggaacg ggaaagacgc
     1441 tcatcgccag agcggtggca ggcgagaccg acgccaactt catctccatc tccgggccgg
     1501 agatcgtatc caagttctat ggcgagagcg agcagcggct gcgccagata ttcgatgaag
     1561 cgtcgaaggc ggctccttcc atcatattca tcgatgagat cgattccatc gctcccaaga
     1621 gggaggaggt ctcgggcgac ctggagcggc gggtggtggc ccagatcctc tccctgatgg
     1681 acggcctctc ctccagggga gaggtgatag tgatagcggc caccaaccgg cccaatgccc
     1741 tcgatcctgc cattcgcagg gggggccgat tcgaccggga gattgagata ggcataccaa
     1801 acagaaacgg caggctggag gtgctctatg tccacaccag gggcatgccc ttggatgaat
     1861 cgctggactt gatggagatc gcagactcga ctcacggctt cgtaggagca gacctttatg
     1921 ccctgtgcaa agaggcggcc atgcgcacac tggaaagggc tctgcccgac ctggacgtga
     1981 aagaggacat accattggat gtgctggaca atctcaatgt gaccagagag gatttcctgt
     2041 ccgccctgaa gaagatcgag ccatcggcga tgagagaggt cttcgtcgag gtggcccagg
     2101 tccattggga tgaagtgggc ggcctggatg aggccaagcg gtccctggtc gaggctgtgg
     2161 aatggccgtt gatgtaccca gaggccttcg cctcggtggg tgtccggccg ccgcggggta
     2221 ttctgctcta cggtcttccc ggtacgggca agaccctcct ggtcagagcc ctggccacgg
     2281 agagcaatgt caacttcatc agcgtcaagg gaccagagct attgagcaaa tgggtgggcg
     2341 agtcggagag ggcggtcaga gagatctttc gcaaagcccg ccaggctgct cctgccctgg
     2401 tattcttcga cgagatcgat tccattgttc ccgcccgggg cagcggatct gactcgcatg
     2461 ttaccgaaag agtggtcagc cagttcctga cggagatgga cggactcatg gagctgaagg
     2521 atgtggttat agtggcggca accaaccggc cggacctttt ggatagctcg ttgctccggc
     2581 cgggcaggtt cgatcgttta gtttatattc ccatgccgga taaagaagct cgtcagaaga
     2641 tactggagat ctatctctca aagatgcccg cctatgaggt ctctgctcaa tggctggcgg
     2701 atatcactga gaacttcagc ggtgccgatc tggagatgct ctgccgggag gcggggatgc
     2761 ttgctttgcg ggagcatatc cggccgggaa tgaaaagaga agagctgata gttgacaaga
     2821 tccttgttac agagaagcat ttccaggagg ccagcgagta tatcaggccg catctgtcaa
     2881 aagatatgct gcagggatac actaagatga tccgtgaatt ccaggtatga aaacttaatg
     2941 atttggaaag agaagagcat ggagacaaag caccaaatat atagtcaaga acaaccaata
     3001 attgctgtga ggaccgaaaa attgacttca gaagacgagt ctaatgaact gctcggcgat
     3061 attaaattcg aggatacgag cagcataacc gtacctgaaa acctcataga tcaggttatt
     3121 ggccaggacg aggcagttga ggtgatcaag aaggcggcaa gccagagaag gcatgtcatg
     3181 ctcatagggt cgcctggtac tggcaagtca atgctcggca aagcaatgag cgagcttctt
     3241 ccggtggagg atctgcagga tgttctggtc tatgccaatc cagaggataa caataccccc
     3301 agggttcgcg tcgttcccgc gggcaggggc aaacagatcg tcgatgccca gaagctggag
     3361 gcaagaaaga aggtccagac gagaaatatg ttcttgatga tcatcgtaat ggcccttatc
     3421 gtctatgcct attatatggg ccagcttctt ttcggaatca ttgccgcagc attgctgttc
     3481 atctctttgc gctatatgct gcccaaagag gatgccatgg tccccaagct gctggtggat
     3541 aacaacggca agaagaaggc gccctatgtg gatgccaccg gggctcatgc cggggcattg
     3601 ctgggagacg tccgtcatga tccttttcag tccggcggcc tggagacgcc atcccacgaa
     3661 cgggttgagt gcggctccat ccataaagcc cacaagggag tgctgttcat cgatgaagtg
     3721 aacaccctcc ggccggagtc acaacagagc ctcctcaccg ccctgcaaga aggggtctat
     3781 caaataaccg gccagagcga gagaagctcc ggcgccctgg tgagaactga gcccgttccc
     3841 tgcagcttca tcatgatcgc tgccggaaac ctggatgccg ttcagggaat gcatcctgca
     3901 ctgcggtcgc gcatcaaggg ctatggttat gaggtctaca tgcgggatac catggaggac
     3961 accctggaga acaggaacaa gctaatcagg ttcgtggctc aggagatcgt gagagacgga
     4021 aagatccccc acttcactca agatgcagtg gcggagatca tgagagaggc caagaggcgt
     4081 tcgggcagaa aggggcatct caccctcatg ctccgcgacc tgggaggcct gatcagggtc
     4141 tctggagatg tagcccgggc ggaatcctct cacctcaccg aggttaatca cgtcatgcag
     4201 gccaagaaga tggcccgttc ggtcgagcag cagctggcgg acagatacat ggagaggcgc
     4261 aaagactaca gcatgttcca ctcctccgga gatgagatcg gcagggtcaa cggcctggca
     4321 gtcatgggcg actcgggcat tgttctgccc atcatggccg agatcacccc tgctcaatcc
     4381 aaggaagagg gcaagatcat cgccactgga aagctgcagg agatcgccaa agaggcggtg
     4441 acaaacgtct ctgtgctgat caagaagttc ctgggcgagg atatcaccaa gaaggatgtg
     4501 catattcagt tcataggcac atatgagggt gtagagggcg atagcgcatc catctccatc
     4561 gccactgctg tcatatccgc tttggaaggg gtgcccgtga aacagacggt agccatgacc
     4621 ggctcgctct ccgtgcgggg ggatgtcatg cctgtgggcg gagtcaccca gaagatcgag
     4681 gctgctgccc aggcgggaat caagactgtc ctcattccca aatccaatat gggggatgtc
     4741 ctgatagatg attccatgaa aaactctatt gagatcatac ctgtatccaa tatcagcgag
     4801 gtattcgagt atgccatggg tagccagagg accaggctga tagagaaact gaaaaagttc
     4861 gccaccgaga agaagatcgg catcaacatc ccagagacga tccccacccc catccggagc
     4921 atctgatcgc ttgaccgcgg agttccatga tacccgcatt ctgcagggga gtcgaaccag
     4981 gatcgttctg gacaatggaa agcttgagga aatagcccag gtgcccttcc agggggcatc
     5041 ggttcgggct ctttttggcg gtgcctgggg ttttgtaacc actgatcggg tggacggtct
     5101 tggccaggag atagatctgg ccaagcgcat tgctcggaag atcggccgga aggaggatct
     5161 cagtctggca gaggctccgc ccggcagaag cgtggtggtg ccagttaaaa gagatccaaa
     5221 agacctatct ttagaggaga aggtagccct tttgagagag atcgaggacg cagcgaaggt
     5281 gaagggcata tcctcgaccc aggcggtcta ctcagagatg gatctggtag cccactattc
     5341 tagttctgag ggcctggatc tggaatcaag gatgaccaga atgggctttg tgatcagcgc
     5401 tgtggctcat aggaatggcc tttatcagac cgatggcgaa ggcagagccg gtgtgggcgg
     5461 cctggagcta ttcgaccggg aagatccaat agctctggcc aggcaggtgg gggagacggc
     5521 ggtcgccctc ctggatgcca aggcggcgag aggagggact caccctgtag tcttggatca
     5581 ggagttggct ggggtgttcg tccatgaggc ggtgggccat gccactgagg gagacataat
     5641 cctggagggg gactcgtgcc tggaaggaaa gctgggccag aggataggat cggagctggt
     5701 gacggtcaag gacgacccca gcctgatgct caacggctac tatcccttcg atgatgaggg
     5761 aagccgagcc caggagacgg ttctggtgga gaatggtgtg cttcggtcat acctcaacac
     5821 ccgcgagacc gcagccaggt taggtggtgt gccgcgcaat gcccgagcag aggggattag
     5881 ccggccggta gtgcggatga gcaataccta catcgccaat ggcgactgga agctggagga
     5941 gatcttagag gagctcaaaa gcggtgtcta tctggcaggg agccgggggg ggcaggtcag
     6001 caccggcgag ggaatcttcc agttcaatgc caagaagggc tatatcgtag agaacgggga
     6061 gaagacgcaa ctgttgcgag atgtatccct atccggaaag atcctggaga cgctcttgcg
     6121 tgtgttggcg gtgggcgacg acctgcggta taacagcggt cgatgcggaa agtccggcca
     6181 gctggtgcct gtgagcgacg gatcgcccca tctgctcgtc gatagggcca cagtgggcgg
     6241 aacaggatag agaagaatcc aagagagata aaattaagat ggtctggatc agctgcggtt
     6301 caatgctcga tccaggctgc tgtttaccat ctccaggctg gtattggtta tctctgcctt
     6361 cagccagtcc caggcggcct gagttgctcg atttagcttc tctcctgcag cgtcctcgat
     6421 ccgggatagt agaccgttct ggctgtcgtt ctgccatcca tcattctgcg tccctttctg
     6481 ctcttgaccg gatccctgca agctctcttc cagcctctct cgcagattct cctccaccct
     6541 ttgcagagta tcagagctgc cgctctctga attatgctcc tctctccagt ttttcggatc
     6601 gaactcattg tccgtctgat tcgccaggat cgcatgaccg gcatccacca gcaagaggtt
     6661 gaagttggga taaggtatgg gctggccata ggctccggct agataggcta ctgcgatcag
     6721 cctgccctca tcatcccgtc cccggttgaa gtcgtcaaca tccaggtaga ccctcttgtc
     6781 catgagcacg gcatatgtga aatccctggc cgctgggccc tggattgcct tcatatccgg
     6841 tgaacggacg tcggccagag tgatctgttc cacactgtct gaaattctgg agtcggcttt
     6901 ctctatcctg aggtcgaatg tatcgccatc gatcacattt gtgactaccc cgtagacctc
     6961 gtctggggag gccgaagatc cgggcaggag ggatagcacc gaaaaaattg tcagtatcag
     7021 aacgaataac cggaggaggt aagatatatt caattccaaa aatctccttt tgccataagc
     7081 tctcatgata cgctcacctt cagctctgca gatcggtttc tgctgccgtt gacggcaaca
     7141 agtctgtata tggtatcctc agatggagat acagatctgg atccctccag ggccaccggg
     7201 ccgatatcct ggtcgatctc gaccaggctt gcgccgacaa cgctccagct gagaacagag
     7261 gactggcccg gtgccaggaa ttccggttcg gcatcgaaga actcgatcac cggatagggt
     7321 gatcccaaga agttcagcgc cagtatccct ccagccatga ttgccagcag cgctgcagtc
     7381 cagatgacaa ccccctgcgg tgttttccag aaggactttt gcatctccca gtaggtcttc
     7441 ttttgagcca tcgtatgcaa atatgagacg attctggtta ttatactctg ctcttcggat
     7501 attgcgagcg ttcaccctct tctgtctgct tgtatctcca tctcttccag agccttcagg
     7561 ctatgctctt tcagtatctg gacgatgcct tc
//
LOCUS       DAOSGP010000037         7591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_8361_length_7591_cov_16.722797, whole genome shotgun sequence.
ACCESSION   DAOSGP010000037 DAOSGP010000000
VERSION     DAOSGP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8361_length_7591_cov_16.722797"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLV49_02045"
     CDS             complement(<1..245)
                     /locus_tag="PLV49_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOG97652.1"
                     /translation="MKLAYYPGCVSLSTGKEMDRSTRAVFSSLEIELEELGDWNCCGA
                     THVSNEMVATGLAARNMSQTELDIVTSCSICYSNLRAA"
     gene            complement(242..772)
                     /locus_tag="PLV49_02050"
     CDS             complement(242..772)
                     /locus_tag="PLV49_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG97653.1"
                     /translation="MATAIEEDFKNEVLRLAGEEVRTCIQCGTCSASCPTAHLMKPSI
                     RRLVKLCLEGRREEALHNETLWLCTSCLLCTVRCPRGIRPKMVVSALKELADREKIES
                     PGKNYDHLFNKQIEDYGRISELPLIGEFLLFYPQGTVQSMKMGLELLPRGKITLEREQ
                     VMGKDEVKRIMEELGK"
     gene            complement(772..1842)
                     /locus_tag="PLV49_02055"
     CDS             complement(772..1842)
                     /locus_tag="PLV49_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG97654.1"
                     /translation="MPKPVDFREREEYPRLMAEAKPNEKCLPCLLCEPVCPTAAIKVT
                     FDKTREDFGPLREGIEGKISIDQEKCNLCGRCARFCKAFLLVDKGEKDRDPRDLVPYE
                     QLLIDEDLCDYCGLCVPLCPEEAIAVEGEPLKLDEEPKIEGKIKVDESLCIGCGRCAL
                     VCPYEGMDISKPFQGEIKMVEKNLVRCDPLGCLACFNVCPAKCWYVDERGKAAPVEDQ
                     CILCGACEKACPVSAIDVQRTEVSHTEVKETPWAEEWKEAIRTILTGERKIPDVSGAV
                     VPPKIDRTPLPAPEAPKVDPELLKMVDEALKPLVPMLAKPKIRQIMENELPDKASQKI
                     VERLHEAERKRKKAREENAGAV"
     gene            complement(1875..>2096)
                     /locus_tag="PLV49_02060"
     CDS             complement(1875..>2096)
                     /locus_tag="PLV49_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG97655.1"
                     /translation="CSICVAICPKKALQEGPLKEIAKGLDAPPVLIDQDLCVFCGMCV
                     NFCPMKAFEMRVVEKKAEPEAAEAAEAKA"
     assembly_gap    2097..2196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2197..4175)
                     /locus_tag="PLV49_02065"
     CDS             complement(<2197..4175)
                     /locus_tag="PLV49_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOG97656.1"
                     /translation="MPIGVYVCHCGLNIAGVLDIEELKSFAEKLPDVAVARDLQFSCS
                     DIGQEQIQKDIAEYKLDRIVVAACSPRLHEPTFRRAIAKAGLNPYMLEMANIREQCSW
                     VHMDLPAMATEKAKDLISMAVAKAALLQPLEQEQIPVNKDVMVVGGGVAGIAASLDLA
                     DAGFHVFLVERRPTIGGYMALLTDVFPTNDCSICVLGPKMSEVFNHPNITLYTYSEVL
                     GVEGSVGNFTVSGVHRARYVDPVLCKGCINECADVCPVEVPNEYDFGIGKRKAIYVPY
                     PQAVPLSACVDPKACIGCGRCVESCPTQAVKLDQVVEHFKINVGAIIVATGWQPFDAA
                     RKEEYGFGRLRDVVTTLQMERMLNASGPTKGEVVRPSTGQVAQSIAFLQCVGSRDATV
                     GNIYCSRICCMASLKNAQLIAEKYPGSDITIYYIDIRAGGEGYEEFYIRAQRLGINFV
                     RARVSSIEEVDGDIYLSFEDTQTGEIKRVRHDIAVLSVGLEPDKSADVLGNLLGLSRR
                     PDRFFEIAHPKMRPVEAHIDGVFIAGCASGPKEIPISIAQGSAAAAKAIRLLQKGFLD
                     LEPTSAYVDPDLCIRCKLCVDVCPQKAISVKSPAYVDEAACKGCGSCAAACPVDAIKM
                     RLFSDEQILAQVRAATEVKKEFPLIIAFLCNWCSYG"
     gene            complement(4270..4845)
                     /locus_tag="PLV49_02070"
     CDS             complement(4270..4845)
                     /locus_tag="PLV49_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HOG97657.1"
                     /translation="MISRVYISYDPDDEDLALRLSLALGRVGLESYVALYNQSPAISL
                     ADRVSFAIRNSECLVALATRKGSRSPTLCQEIGLARGIDQLIIPLQEEGAQLPFLIDQ
                     LRALSFRRESFADAIGILIKSIRELSRLEWLRVVCPQCGEEMTQYLTDQDEVDSAQEK
                     GIFLETICSYCQNTILLDPRTFEPLPGSKNR"
     gene            complement(4853..>7591)
                     /gene="fdhF"
                     /locus_tag="PLV49_02075"
     CDS             complement(4853..>7591)
                     /gene="fdhF"
                     /locus_tag="PLV49_02075"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOG97658.1"
                     /translation="LLIDDREVSVPQGSTILDAARIAGSYVPSLCHHPDLKPIGSCKL
                     CIVSVEGLDYYPLSCNTPAAEGMVVHTRTEELQEMRRHTLEMLLALTNHPTSCLFCER
                     KNECGELRECMRKFPVTVGCKYCPNDGQCELQEVVTFTGLDRIRYQTSFRDMPVLREP
                     FFDRNYNLCIMCSRCIRMCDEVRGLGILFANPDFHRNHWIGPESLQDADCKFCGACVD
                     ACPTGALFIRSEKWIRPDRTVVTICPYCGVGCSLDLKISEDRILSVTPSRDGAVNRGQ
                     ACVKGHFGLEEIIHHPDRFTTPLIKRNGQFEPASWDEALDLVARRFTEIQDRYGPDSL
                     GALSSSRCTNEENYLVQKLARTAFKTNNVDNCARVCHAPSVTGLAACFGSGAATNSFN
                     QIEDADVLFIIGSNTTEAHPVVSLKIIKAARNGAKIIVADPRKIELVDRASVWLNQKP
                     GTSIALINAMMNVILKEGLEDRDFIDRRTMGFENLQKTVEEYPPERASQITGVAVEDI
                     IEAARLYARADKAMIIYGLGITEHTTGTSNVMSLGNLAMLTGNVGRRSTGIMPLRGQN
                     NVQGACDMGALPNVHVGYQPVTDAGIRSKFEEAWKVDLPSVPGQTSTQMIERNPGKEI
                     RGLYILGEDPAHTEPDTNRVRSTLESLDFLVVQEIFPTETTRFADVILPAASFAECDG
                     TFTNGERRIQRVRKAIPALCGQENWLTICQIAQRMGYPMSYGHPSDIMDEIASLAPMF
                     AGVKFDRLDDGGLQWPIPSLDHPGTETLHAESFSCGLGRFNAVQHKYPAEQTDAEYPL
                     ILTTGRRREHYNCGSMTRRSKGIMWVWPEEAIEISPADARELGIEDGETVVVSSRRGS
                     VKTKARVTDKSSRGMAFMSFHYQDVLTNLLTNAALDPQAKTPEYKACAVKIEKIKA"
BASE COUNT         1507 a         2238 c         2039 g         1707 t
ORIGIN      
        1 gccgccctga ggttgctgta gcagatggag caggaggtca cgatatccag ctcggtctgg
       61 gacatgttcc gggcagcaag ccccgtggcc accatctcat tggagacgtg agttgccccg
      121 cagcagttcc aatcccccag ctcctccagc tcgatttcaa ggctcgaaaa gaccgctctg
      181 gtggacctgt ccatctcctt tcccgtggag agagaaacgc aacccggata gtaagccagc
      241 ttcatttgcc cagctcctcc ataatcctct tgacctcatc ctttcccata acctgctctc
      301 tttccagggt tatcttgccc cgggggagaa gctccagccc catcttcatg gactgaacag
      361 tcccttgcgg atagaagagc aagaactcgc cgatgagagg aagctcgctt atccggccgt
      421 aatcctcgat ctgcttattg aagaggtggt cgtagttctt tccagggctt tctatcttct
      481 ccctgtctgc cagctccttc agagcggaca cgaccatctt cggccttatt ccccggggac
      541 aacgcacagt gcacaatagg caggatgtgc acagccacag agtctcattg tgcagggcct
      601 cctcccgtct gccctccaga cagagcttga ccaatcttct tatgctcggt ttcataagat
      661 gagccgtggg acagctggcc gagcaggtgc cgcactgaat gcaggtcctt acctcctctc
      721 ctgccagcct taatacctcg ttcttgaaat cctcttctat tgccgtggcc attacactgc
      781 ccccgcattc tcttctcttg ccttctttct cttcctttca gcctcgtgga gcctctctac
      841 aatcttctgg ctcgctttat ccggcagctc gttctccatg atctgacgga tcttgggctt
      901 ggccagcatt ggcaccagcg gctttaaagc ttcatccacc atcttcagaa gctcgggatc
      961 caccttcggc gcctccggag caggcagtgg cgtcctatcg atcttgggcg ggaccacagc
     1021 gccgctcaca tccggtatct tccgctcgcc ggtcaatatg gtacggatcg cttccttcca
     1081 ctcctctgcc cagggggtct cctttacctc cgtatggctg acctcagtcc tctgcacatc
     1141 aatggccgag acgggacagg ccttctcgca ggccccgcat aggatgcact gatcttccac
     1201 cggagcggct ttgccccgct cgtccacata ccagcacttg gcagggcaga cgttgaagca
     1261 ggccaggcag cccaggggat cgcatcgaac gaggttcttc tccaccatct tgatctcccc
     1321 ctggaagggc ttggatatgt ccatgccctc ataggggcag accagagcgc atctgccgca
     1381 gccaatacat aggctctcat cgacctttat cttcccctcg atcttcggct cctcatcaag
     1441 ctttagaggc tcgccctcga cagcgatcgc ctcctccgga cagaggggga cgcaaagccc
     1501 gcagtagtcg caaagatcct cgtctatcag cagctgctca taaggcacca gatcgcgggg
     1561 atcccgatcc ttttcccctt tatcgaccag aaggaaggcc ttgcagaacc tggcgcaccg
     1621 tccgcagagg ttgcacttct cctgatctat ggagatcttc ccttcaatcc cctcccggag
     1681 gggtccaaaa tcctcccgag tcttgtcaaa ggtcaccttg atcgctgccg tcgggcagac
     1741 gggctcgcag agcaggcagg gcagacactt ctcatttggc ttcgcctcgg ccatgagcct
     1801 gggatactcc tccctctctc tgaagtcgac cggctttggc attgcctttt ctgttgtctc
     1861 acacacattt tcagtcatgc ttttgcttcg gcagcctctg ccgcctctgg ctcagccttt
     1921 ttctccacaa ctctcatttc gaaggccttc attggacaga agttcacgca catcccgcag
     1981 aatacgcaaa gatcctggtc tatcaacaca ggcggcgcat ccaggccttt ggcgatctcc
     2041 ttcagcggcc cctcctgcaa ggcttttttg gggcagatgg caacgcaaat ggagcannnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgt agctgcacca gttgcacaaa
     2221 aatgcgatga taaggggaaa ctccttcttc acctcggtcg ccgctctgac ctgggccagg
     2281 atctgttcat ctgagaacag acgcatcttg atggcatcca ctggacaggc ggcagcacac
     2341 gacccacagc ctttgcaggc tgcctcgtcc acgtaagccg ggctcttcac agatatggcc
     2401 ttctgagggc agacatcgac gcacaacttg cacctgatgc acagatcggg atccacatag
     2461 gctgatgtgg gctccagatc caagaatccc ttctgcaata gccttatggc cttggcggcg
     2521 gcagcactgc cctgagcgat ggatatgggg atctctttgg gcccggaggc gcacccggcg
     2581 atgaacaccc catcgatatg agcctctacc ggcctcatct tggggtgagc gatctcaaag
     2641 aacctgtccg gcctgcggga gaggccgaga aggttgccca gaacgtccgc actcttatcc
     2701 ggctccaggc ctacggagag gacggcgata tcgtgccgga ccctctttat ctctccagtc
     2761 tgggtgtcct caaagctgag atagatatcg ccatctacct cctcgatgct ggatactcga
     2821 gcgcgaacga agtttatgcc cagcctctgc gcccgtatat aaaactcctc ataaccttct
     2881 ccacccgccc tgatatcgat ataatagatg gtgatatccg agccggggta cttctcagcg
     2941 atcagctggg cattcttcag cgaggccatg cagcagatcc gagagcaata tatgttgccg
     3001 actgtggcat ctctggagcc gacacactgc aagaaagcta tggactgggc gacctggccc
     3061 gtgctcggcc ggacaacctc gcccttggtc ggaccggagg cattgagcat cctctccatc
     3121 tgcagagtgg tgacgacatc cctgagcctg ccaaagccgt actcctcctt ccgggcggcg
     3181 tcaaagggct gccatccagt ggccacgatt atcgccccca cattgatctt gaagtgctcg
     3241 accacctgat ccagcttgac cgcctgggtg gggcaggact ccacgcaccg gccgcagccg
     3301 atgcaggcct tggggtctac gcatgccgac agggggacgg cctgaggata gggcacatag
     3361 atggcttttc tcttgccgat gccgaagtcg tactcattgg gcacctccac tggacagacg
     3421 tctgcgcatt cgtttatgca gcctttgcag aggaccgggt ccacatagcg tgcccggtga
     3481 actccagata ctgtgaagtt ccccacgctg ccctccaccc ccaggacctc ggaataggtg
     3541 tagagggtga tattgggatg gttgaagacc tcggacatct tggggcccag aacgcagatg
     3601 gagcagtcgt tggtgggaaa gacgtctgtg agcagggcca tgtaaccgcc gatggtcggc
     3661 cggcgttcca ccaaaaacac atggaagccc gcatccgcca ggtccaggga ggcagcgatc
     3721 ccggccacgc ctcctcctac caccatcaca tccttgttga ccggtatctg ctcctgctct
     3781 aagggctgca ggagggcagc cttggccact gccattgata tcaggtcctt ggccttctcc
     3841 gtggccatgg ctggcaggtc catgtgcacc caggagcact gctcccggat gttggccatc
     3901 tccagcatat aggggttcag cccggccttg gcaatggccc ggcgaaacgt gggctcatgc
     3961 agtcggggcg agcaggcggc caccacgatc ctatccagct tatattcagc aatatccttt
     4021 tgaatctgtt cctgcccaat atcagagcag gagaactgca gatctctggc cacggccaca
     4081 tcaggcagct tctcggcgaa gctcttcagc tcttcaatat ccaagacgcc ggcgatattc
     4141 aggccgcaat gacatacata aacaccaatg ggcatggact tcaggtagta tgatctcctt
     4201 aattcactta aacctatgca agacaaaaag aagccatttg tcaaaaattt tccatgaact
     4261 tctggggttt cagcgattct tgctcccagg cagaggttcg aatgtccgcg ggtcgagcag
     4321 gattgtgttc tgacagtagc tgcagatcgt ctccaggaag attccctttt cctgggcaga
     4381 gtccacctca tcctggtcgg tcagatactg ggtcatctcc tctccacatt gggggcagac
     4441 cacccgaagc cactccagac ggctcagctc ccggatgctt ttgatcaaga tgcctatggc
     4501 atcggcaaag ctctcccttc tgaaggataa agctcgcaat tgatcgatca gaaaaggaag
     4561 ctgagcgccc tcctcttgca agggaatgat cagttggtca atgcccctgg ccagacctat
     4621 ctcctggcag agggttggag aacgacttcc cttccgggtt gccagagcca ccaggcactc
     4681 agagttcctt atggcaaagg acaccctatc ggcaagagag atagcaggcg attgattgta
     4741 cagggcgacg tagctttcca ggcccacccg ccccagcgct agcgagagcc gcagagccag
     4801 gtcttcatca tccggatcgt aggaaatgta gactctagag atcatccaat cattacgcct
     4861 ttatcttttc tatcttcaca gcgcatgcct tgtactccgg agtcttcgcc tgaggatcaa
     4921 gagcggcatt ggtcagaagg ttggtcaaga catcctggta atggaatgac atgaaggcca
     4981 tgccgcggga ggacttgtct gtaaccctag ctttggtctt aacagagccc cgcctggagc
     5041 tgactacaac tgtctctccg tcctcgatcc cgagttctct ggcatcggca gggcttatct
     5101 ctatggcctc ctccggccat acccacatta ttcctttgga tcgcctggtc atggatccgc
     5161 agttgtaatg ctcccttctc ctgccggtgg tgagaatcaa gggatattcc gcatcggtct
     5221 gctcagcggg gtacttgtgc tgtacggcat tgaaccttcc caggccgcag ctgaagctct
     5281 ccgcatgcag ggtctctgtt cccggatggt caagggatgg tatgggccac tgcagcccgc
     5341 cgtcatccag ccgatcgaac ttcactccag caaacatcgg cgccagtgag gcgatctcgt
     5401 ccatgatatc cgaagggtgg ccgtagctca tcggatagcc catcctctgg gcgatctggc
     5461 agatcgtcag ccagttctcc tggccgcata gtgctggtat cgcttttcgc accctttgaa
     5521 tgcgccgctc gccgttagtg aatgttccgt cacactcggc aaagctggct gcgggcagga
     5581 tgacgtctgc aaacctggtg gtctcagtgg gaaatatctc ctgcactacc aggaagtcca
     5641 gggactccag agttgatctc acccggttgg tgtcgggctc ggtatgggcg gggtcctctc
     5701 ccaggatgta aaggccgcgg atctcctttc ccggattgcg ctctatcatc tgggtggagg
     5761 tctggccggg gacagagggc aagtcaacct tccaggcttc ctcgaacttg gaccggatcc
     5821 cagcatcggt taccggctgg tagccgacat ggacattggg aagagccccc atatcgcagg
     5881 ctccctggac gttgttctgg ccgcgcagag gcatgatgcc agtcgagcgg cggccaacgt
     5941 ttccggtgag cattgccagg ttgcccaggg acatcacatt ggaggttcca gtggtgtgct
     6001 cagttatgcc caggccgtag atgatcatcg ctttatcggc cctggcatag agccgggcgg
     6061 cctcgatgat atcctctacg gccactccgg tgatctggga tgccctttca ggcggatact
     6121 cctccaccgt cttttgcagg ttctcaaatc ccatggtgcg ccggtctatg aaatcccggt
     6181 cctccagccc ttctttaagg atcacattca tcattgcatt gatcagggcg atgcttgttc
     6241 caggtttctg gttgagccag accgatgccc tgtcgaccag ctcgattttg cgggggtcag
     6301 cgacgattat cttcgcacca tttctggctg ccttgattat cttcaggctg actacggggt
     6361 gggcttcagt ggtattggag ccgatgatga aaaggacatc ggcgtcttca atctggttaa
     6421 aggagttggt ggctgctccg ctgccaaagc aggcggcaag accggtcact gagggggcat
     6481 ggcagacccg ggcgcagttg tccacattgt tggttttaaa ggccgtccgg gcaagctttt
     6541 gcaccagata gttctcctca ttggtgcagc gggaggagga gagggcccct aaagaatcgg
     6601 ggccgtatcg atcttggatc tctgtgaacc ttcgggcgac caggtctaaa gcctcatccc
     6661 aggatgccgg ctcgaattga ccgtttctct ttatcagagg agtggtgaag cggtcgggat
     6721 ggtggatgat ctcctccagt ccaaagtggc ctttgacgca ggcctggccc cggtttaccg
     6781 ctccatcccg gcttggcgtg acggataata ttctgtcctc agagatcttg aggtccaggc
     6841 tgcagcctac gccgcagtag gggcagatgg tgaccacggt gcggtcgggc cggatccatt
     6901 tctccgaacg gatgaagagg gcgccggtgg ggcaggcgtc cacgcatgcg ccgcagaact
     6961 tgcagtctgc atcctgcagg gattcgggac ctatccagtg gttgcggtga aaatcggggt
     7021 tggcaaatag gatgcccagg ccgcgtactt cgtcgcacat tcttatgcac cggctgcaca
     7081 tgatgcagag gttgtagttg cggtcgaaga acggctctct taataccggc atatcccgga
     7141 aagaggtctg atatctgatc ctgtccaaac cggtgaaggt gacgacctct tgcagctcgc
     7201 actggccgtc gttaggacag tatttgcagc ccactgttac tggaaacttg cgcatgcact
     7261 cccgcagctc gccgcattcg ttctttctct cgcagaagag gcaggaggtg ggatggttgg
     7321 tgagggcgag gagcatctcc agggtgtgtc gtctcatctc ctggagctcc tcggtgcggg
     7381 tatggacaac cattccctcg gctgcaggag tattgcagga taaaggatag taatccaggc
     7441 cttccacaga gacgatgcag agcttgcagg agcctatggg cttcaagtcc gggtggtggc
     7501 aaagagacgg cacataagag cccgcaatgc gggcagcatc gagaatggtg gacccctggg
     7561 ggacggatac ctctcgatca tcgatgagca g
//
LOCUS       DAOSGP010000038         7544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_8441_length_7544_cov_15.948858, whole genome shotgun sequence.
ACCESSION   DAOSGP010000038 DAOSGP010000000
VERSION     DAOSGP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8441_length_7544_cov_15.948858"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..783
                     /locus_tag="PLV49_02080"
     CDS             238..783
                     /locus_tag="PLV49_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase"
                     /protein_id="HOG97659.1"
                     /translation="MRTVVLDVLLLRRTLARSPVRWVMHMSMFYGFLALAALSGLGLM
                     LDIVEHFNLAGFAHEAEMVKEMLALPFDVFGYLLLFGSTIAISRRILIKFVRDNTSAY
                     DALLIGAVFLITVTGFYAEWMRGNSFLVGNMFEYPIYAPHFALIHTLLALGLFALILP
                     WSKYIHVIATPMTLIANRGGE"
     gene            785..2155
                     /locus_tag="PLV49_02085"
     CDS             785..2155
                     /locus_tag="PLV49_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOG97660.1"
                     /translation="MADNKESKPLMTGFMMSTQLLEVDGCNRCQECMKWCPTFAVRPD
                     RPGITPMYKNARWRELMAKSHSLRAKIFGAKPLNDDEMKVFTEDTYSCTTCGVCGTVC
                     EAGIRTVELWEAMRPNLVARGDGPVGKQSFFPKLVKADRNPYQAKQEERLCWVPKDIK
                     VQESGEIVYFAGCTAAYRQQALGVATVRILDNLGVPFAMLGKDEWCCASAMVRTGQRD
                     VMAEHAVHNVDALKDAGAKNVLFACAGCLRNAAVDWPLWYEGYIPYETMPLSVYLRDR
                     IRKGDVKYKIPLNYSVTYHDPCHNGRHLMHLKGKDWSFEASRDCITSIPGVKFRDMVR
                     NRALQRCCGAGGGVKAGIPDLALDCAKMRVKDGEEVSAEIMASTCPFCRRNIMDGRND
                     AKSGIKVLDVVELMAASMGLDMTIPENPYTKFQEQDVLVCGPAECKLDTVERKEPGKD
                     LVGEAH"
     gene            complement(2210..3355)
                     /gene="cofE"
                     /locus_tag="PLV49_02090"
     CDS             complement(2210..3355)
                     /gene="cofE"
                     /locus_tag="PLV49_02090"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOG97661.1"
                     /translation="MGIKSDLVRPGDDLVEFLVLAMNRSGQAFQDGDILVVSESIVAT
                     SEGRVVDLDKIQPGDLAISLAGQYKKDPREMELILRESDEIVGGIPGVVLTLNNGFLF
                     PNAGIDNSNAPPGHVVLFPADPKGSAIAIRERMANGKKIGVIIGDSRTHPLRLGCVGV
                     ALACSGLEAVEDARGQKDLFGRELKITRKAIADNLVSAAQIVMGEGDEGIPAAIIRDS
                     GVPIKEASGEIPTIPPAECMYIGALGIGPRPYAGGYDQLIECAGQAIARAYAPYSRFR
                     VGAALLTKKGNVYSAGNIENASTGAGICAERVAISQAIASGEREFEAIAIVGDGCQPI
                     SPCGICRQSLIEFGEDIIVIMANCKGDALTASSRDLLPRAFTGKWLE"
     gene            complement(3369..4625)
                     /gene="folP"
                     /locus_tag="PLV49_02095"
     CDS             complement(3369..4625)
                     /gene="folP"
                     /locus_tag="PLV49_02095"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOG97662.1"
                     /translation="MIEGLVGWVKVGDLQPVRLMGVINLSRESFYKGSVASSNEALAT
                     ARRLQDEGADIIDLGAVSTAPGSPPISEEVEKDRLFAALKEITDNLDIVVSVDTQRAK
                     IAEKALSCGATCINDVSGLHDPDMASKVAEYDGSLIIMASDQRPGDLPLLNQIIPLLG
                     DKVRQAVGAGVALNKISIDPGIGKWMPERTAAQDLAILDGFDRLRVIGRPVVAALSRK
                     TFIGESLNIPDPGGRLAGSLAATAVAVYLGAHIVRTHDISASLDAIAMARAIRGRPIV
                     TSDCSMDCDVDEHAVGDMQVEALGYLGQGMDLAEHLKRIQVDEGAYQLLSRKGSFRIL
                     AVRGLSSMEALIIKQEMLARGGDAAIPKMALRCDKRPQEILILGTHAQISSLVRNLKG
                     QPFRLGHLAAIIEEALELIDRPERYR"
     gene            complement(4658..5443)
                     /locus_tag="PLV49_02100"
     CDS             complement(4658..5443)
                     /locus_tag="PLV49_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG97663.1"
                     /translation="MSWQGELNKYLASAYKNWVISKRNVFTLFELFFWPLVNLLSIGL
                     LTRFLMVDESMVSFLLVGSIALTILQVAQMDVAYVLLFDMWSKSIKHTFIAPIRSRHL
                     IFGSLFFGIMRGSVVFAILASISFYLFDFRFQAGGLIPPLIFLAGVFSMAAIIGITVC
                     ISILTFGQKADVAAWSLSGLILLFSGIYYPVDVLPFFLQVLARAIPLTYFLEYYRSIF
                     IPGPHHLAVGLSLAALYLLLGLILFDRAIRRARRTGILLWLSE"
     gene            complement(5427..>5664)
                     /locus_tag="PLV49_02105"
     CDS             complement(5427..>5664)
                     /locus_tag="PLV49_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG97664.1"
                     /translation="ITYRGLANLDALRALEGVLAVRGNPGIVDGQIDRQLDILMDSNE
                     DSLDRILKSFQDAKILDINIEEPDLEDVFLELAR"
     assembly_gap    5665..5764
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5765..6538)
                     /locus_tag="PLV49_02110"
     CDS             complement(<5765..6538)
                     /locus_tag="PLV49_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG97665.1"
                     /translation="MSEKSVQARGICKSYKSLFGSAKTVLEGISLDIDREEIFGLLGP
                     NGSGKTTLISIFSTLIYPEKGSLSILGLNARRDRGEIRKIINISTAKPNFPWSLTVRE
                     NLVHYGMLYGLYGLGLNRTVEDQIDAFELGEYRDVKFENLSTGLKQRLSLAKAMLNHP
                     RLLFLDEPTTGLDPDMSAKTHDLIRRIHDEEGISVIMSTHYMPEAELLCDRIAFLRKG
                     RVAALDTPQNLKNHLELGKRVKITYRGLANLDALRALEGV"
     gene            complement(6576..>7544)
                     /locus_tag="PLV49_02115"
     CDS             complement(6576..>7544)
                     /locus_tag="PLV49_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97666.1"
                     /translation="KIICILLILVGLGQAEDIIFFADDHYKSISHLQLNASVTNPALS
                     PGDGVLRISIANQGRIEELIPISSSDSPDDILQEQREEMNSSLAMDVRAALQSAGPIQ
                     VTSGVQHIAILPAGEARELQFNISIDRRASGWYDLPLQVDYVRQVDVSVSDGEAFPLR
                     QPSNQSLNLRVYAFGDKDDLRIAGVRSHLYPGGEGSLIIAVENIGEDIVPNCSASLIA
                     APPFRVKSRDLPLGDLSPGEMASASFLVEVDGDAAWEEYQLGLGLRSKKMDLVLPFEL
                     SLKGSGRLISNGAILFIALLALLVLLAIILRRQKLLYGRKRRIKRL"
BASE COUNT         1661 a         2078 c         2004 g         1701 t
ORIGIN      
        1 tagtgataaa tataacgagg aggatatgag atggcattct acacgttggg cctcaccttt
       61 gaacttgtga tactcctaac agttattgta cttgctgttt ggatatatgg tatatatttt
      121 aactttaaga aatggggcct cggatccacc ggctaccacg acccgttgcg cagaagcttc
      181 tggttattcc ttgccacctg gattcatgag gcatttaagg acggcatttg ggtatttttg
      241 aggacagtgg tgcttgatgt tcttcttctc aggaggactc ttgccaggag cccggtacga
      301 tgggtaatgc atatgtccat gttttatgga ttcctggcgc tggcggcact ctccggcctt
      361 ggactcatgc tcgatatcgt ggagcacttc aatttagccg gatttgccca tgaggcagag
      421 atggtaaagg agatgctggc actgcccttc gatgtattcg gatatcttct gctattcggc
      481 tccaccattg ccatatccag aaggatcctg atcaagttcg tgcgggataa caccagcgca
      541 tatgatgccc tcttgatcgg agcagtcttc ttgatcactg tcactggatt ttacgctgaa
      601 tggatgcgcg gcaactcttt cctggtaggg aacatgtttg agtatcccat ctacgcgccc
      661 cacttcgctc tgattcatac cttgctcgcc cttggtctgt tcgccttaat tctgccctgg
      721 agcaagtaca tccacgtgat tgccacgccg atgaccctga tagccaaccg aggaggagag
      781 taaaatggct gataacaaag aatcaaagcc cttgatgacg ggctttatga tgtccaccca
      841 gctcctggag gtagatggct gcaacagatg ccaggagtgt atgaagtggt gcccgacatt
      901 cgctgtcaga cctgaccggc ccggcatcac gcctatgtac aagaacgccc gctggagaga
      961 gctgatggcc aagagccata gcctgcgagc caagatcttc ggagccaagc cgctcaacga
     1021 cgatgagatg aaggtcttca ctgaggacac atacagctgc accacctgcg gcgtctgcgg
     1081 aacggtctgc gaggctggaa tcaggactgt agagctctgg gaggcaatga ggccgaacct
     1141 ggtggccaga ggagacggtc cagtgggaaa gcagtccttc ttccccaagc tggttaaagc
     1201 ggatagaaat ccctatcagg ccaagcaaga ggaaaggctg tgctgggttc ccaaggatat
     1261 caaggtgcag gagtcaggag aaatagtata cttcgcaggc tgcacagccg cctaccgcca
     1321 gcaggcgttg ggagtggcca ccgtccggat cctggacaac ctcggtgtgc ccttcgcgat
     1381 gctgggcaag gatgaatggt gctgcgcttc agccatggtc aggaccgggc agcgggatgt
     1441 catggccgag catgccgtgc ataatgtcga tgctcttaag gatgccggag caaagaacgt
     1501 cctgttcgcc tgcgctggct gtctcagaaa tgcggcagtc gattggccgt tgtggtacga
     1561 gggttatatc ccctacgaaa ccatgccact gtcggtttat ctcagagata ggataaggaa
     1621 gggagatgtg aagtacaaga tccctctgaa ctattcggtc acataccatg atccatgcca
     1681 caatggcagg cacctgatgc atctcaaggg aaaggattgg tcctttgaag catcccgcga
     1741 ctgcatcaca tctatcccgg gagtgaagtt cagggatatg gttcgaaaca gggctcttca
     1801 gaggtgctgc ggtgcaggcg gcggcgtcaa ggcaggcatt cccgatctgg ccctggattg
     1861 cgctaagatg agggtcaaag acggtgagga ggtctctgcg gagatcatgg catccacctg
     1921 tccattctgc agaaggaaca tcatggatgg cagaaacgat gccaagtccg gcataaaggt
     1981 cttggacgtg gtggagttga tggctgcatc catgggactg gatatgacca tccccgagaa
     2041 tccgtacacc aagttccagg agcaggatgt cctggtctgc ggcccggccg agtgcaagct
     2101 cgataccgtg gaacgcaagg agccaggcaa ggatctggtg ggcgaggctc actgaaaaga
     2161 tcaggatgcc cggagttccg gacatcttct tttccatttt ttaaagattc tattccaacc
     2221 acttgcccgt gaaggcccgg ggaagaaggt cacgggagga ggcggtcaga gcatctcctt
     2281 tgcagttggc cattatcacg atgatatctt ccccgaactc tatcaggctc tgcctgcaga
     2341 tgccgcaggg ggatataggc tggcagccat ctcctactat cgctatcgcc tcaaactccc
     2401 tctctcctga ggctatagct tgagagatag ccactctctc tgcgcatatc cccgcgccgg
     2461 tggaggcgtt ctctatgttt cctgcggaat agacgtttcc ctttttagtg aggagggcag
     2521 cgccgaccct gaagcgggag taaggagcat atgctctggc tatggcctga ccggcgcatt
     2581 cgatcaattg gtcgtatcct ccggcatatg gtctggggcc gatcccaagg gcgccgatat
     2641 acatgcattc cgctggcgga atggtgggaa tctcgccgct ggcctccttg atgggaacgc
     2701 cagagtctct tattatcgct gctggaatac cctcgtctcc ctctcccatc acgatctgag
     2761 cagcggatac cagattgtcg gctatggcct tgcgggtgat cttgagctct ctgccgaaga
     2821 gatccttctg cccccgtgca tcctccaccg cttcaaggcc ggagcaggcc agcgccaccc
     2881 caacgcagcc cagccggagt ggatgagttc tactgtctcc gataatcacc cctatcttct
     2941 ttccgtttgc cattctttcc cggatggcta tggcagagcc tttgggatcg gcgggaaata
     3001 ggaccacatg acctggtggg gcattggagt tgtcgatacc agcattggga aagagaaatc
     3061 cattgttcaa tgtcaggacc actccaggga tgcctcccac gatctcgtcc gactccctga
     3121 ggatcagctc catctctcga gggtcttttt tgtactggcc ggccagagat atggccagat
     3181 cgcctggctg gatcttatcc agatcaacca cccgtccctc agaggtggcg acaatgctct
     3241 cggagacgac cagtatatct ccatcctgga aggcctgacc agacctattc attgctagca
     3301 ccaggaactc cacaaggtcg tctcccggcc tcaccaagtc cgatttaatt cccaaaacct
     3361 gcaataaatc acctgtaacg ctctggtctg tcgatcagct ccagggcctc ctcaatgata
     3421 gcggccaaat ggccgagccg gaaaggctgc cctttcaggt tcctgaccag gcttgatatt
     3481 tgagcatgag tgccgaggat taaaatctct tgcggccgct tgtcgcagcg cagggccatc
     3541 tttggaatgg cggcatctcc tccccgggcg agcatctcct gctttattat cagggcctcc
     3601 atgctgctca ggccccgcac cgccaggatc cgaaaagatc ccttccggga gagaagctgg
     3661 tatgcgccct cgtccacctg tatccgcttc agatgctctg ccagatccat tccctggccg
     3721 agatagccta aagcctctac ctgcatatcg ccaactgcat gttcatccac atcgcaatcc
     3781 atactgcaat cgctggtcac aatcggccgt ccgcggatgg ccctggccat ggcgatcgca
     3841 tccaggctgg cggagatgtc gtgcgttcgg acgatatgcg cccccagata gacggctact
     3901 gccgttgctg ccaggcttcc ggcaagcctc cctccaggat cgggaatgtt caggctctcc
     3961 ccgatgaatg ttttacggga taaagcagcc actaccggcc gcccgatgac gcgcagccgg
     4021 tcaaagccgt ccaagatggc cagatcctgg gctgccgtcc tctcgggcat ccacttgccg
     4081 atccctggat ctattgagat cttgttcaga gcaactcctg ctcccacggc ctgcctcact
     4141 ttatctccca ggaggggaat gatctgattc aacaagggca ggtctccagg cctttgatct
     4201 gatgccatga tgatcagaga gccgtcatat tctgctacct ttgaggccat atcaggatcg
     4261 tgcaggccag agacatcgtt gatgcaagta gctccacagg acagtgcctt ttctgcaatc
     4321 ttcgccctct gggtatccac ggagaccacg atatccaagt tatccgtgat ctcctttaag
     4381 gcagcaaaga gcctgtcctt ctccacctct tcgcttatgg ggggagatcc gggggctgtt
     4441 gagaccgcac ccaggtcgat gatatcagct ccctcatcct gcagtcgcct ggcagtagct
     4501 agagcctcat tcgagctggc gactgagccc ttatagaacg actcccggct gaggttgatg
     4561 acccccatca gccgcacggg ctgaagatct ccaaccttta cccaacccac caagccttca
     4621 atcactttca acaccactag ctcaatgatt tcattcccta ttccgagagc cagagcagga
     4681 ttcccgttct cctcgcccgg cggatcgccc ggtcgaagag gatgagcccc agcagcagat
     4741 atagcgcggc cagagataaa cctacagcca gatgatgggg tccggggata aatattgacc
     4801 tgtaatactc caggaaatag gtcagaggga tggccctggc caagacctgc agaaagaatg
     4861 ggaggacatc caccggatag tagatcccgc tgaagaggag tatcaggccg ctcagactcc
     4921 aggcggcaac atcagccttt tggccgaagg tgaggataga gatgcagacc gtgattccta
     4981 tgatcgcggc catggaaaag actcccgcca gaaatatcag cggggggatc agcccgcccg
     5041 cctgaaacct gaaatcaaac agatagaagc tgatcgatgc caggatggca aagaccaccg
     5101 atcccctcat gatgccgaag aacagggatc caaagatcag atgccgactg cgtatcgggg
     5161 cgatgaatgt gtgcttgatg ctcttggacc acatatcaaa gaggaggaca taggccacat
     5221 ccatctgcgc cacctggaga atggtgagag cgatggaacc taccagcaga aaggagacca
     5281 tcgattcatc caccatcaga aatctagtga gaaggccgat ggagagcagg ttgaccaggg
     5341 gccagaagaa cagctcgaag agggtgaaga cgttcctctt ggagatcacc cagttcttgt
     5401 aggctgaggc cagatacttg ttcagctcac cttgccagct caaggaatac gtcctccaga
     5461 tcgggctctt ctatattgat atccagaatc ttggcatctt gaaagctttt cagaatcctg
     5521 tccaggctat cctcgttact gtccatcaag atatccaact gtctgtcaat ctgaccatcc
     5581 actattccgg ggttgcccct gaccgctaag accccctcca gagctctcag ggcatcaagg
     5641 ttcgccagcc cccggtaggt tatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnngacccc ctccagagct ctcagggcat caaggttcgc cagcccccgg taggttatct
     5821 tcaccctctt tcccagttcg aggtggtttt tcaagttctg aggggtatcc agggctgcca
     5881 ctctgccctt tctcagaaag gcaattctat cgcagagcag ctccgcctcg ggcatatagt
     5941 gggtggacat tataactgag atcccctcct catcatgaat tctcctgatc agatcgtggg
     6001 tcttggcgga catgtccggg tccaggccgg tggtcggctc gtccaaaaaa aggagtctgg
     6061 gatggtttag catggccttg gccagagaca ggcgctgctt taagccggtg gagaggttct
     6121 cgaacttcac atccctgtac tcccccagct caaaggcatc gatctggtcc tcgaccgtcc
     6181 gattgaggcc aaggccataa agtccgtaga gcattccata gtggacgagg ttctctctta
     6241 ccgtcaggct ccaggggaag ttgggtttgg cagtgctgat attgattatc ttgcggatct
     6301 ctcccctgtc ccgtctggca ttcaatccca gaatggacag gctccccttc tccgggtaga
     6361 tcagagtgga gaatatggat atgagagtgg ttttccccga gccgttgggg ccgagcaggc
     6421 cgaagatctc ttctcgatct atatccagag atattccttc caggaccgtc ttggcggagc
     6481 caaagaggct cttgtagctc ttgcatatcc ccctggcctg gaccgatttt tcgctcatct
     6541 tcccgatctt cctttgtatt tctcaggctt tccgcctata gccgcttgat ccttctcttc
     6601 cggccataaa gaagcttttg tctgcgcagg atgatggcga gcagaacaag aagggccaat
     6661 agggcaatga agagaatggc cccattggaa atgaggcggc ccgatccctt taagctcagc
     6721 tcaaagggga ggaccagatc catctttttc gagcgcaggc ccaggccgag ttgatactcc
     6781 tcccaagcgg catctccatc aacctcgacc aggaagctgg cggaggccat ctcacccggc
     6841 gacaggtctc caagaggaag gtctctgctt ttcacccgaa agggaggagc agctataagg
     6901 ctggccgaac agttcgggac gatatcctct ccgatgttct ccaccgcgat tattaaagat
     6961 ccttctcctc ccggataaag gtgagaccgc actcctgcaa tgcgcaggtc atccttatct
     7021 ccgaatgcat agaccctcag attcaggctc tggtttgatg gctggcggag gggaaaggcc
     7081 tcgccgtcgc tcacgctcac gtccacctgg cgcacataat ccacctgcag aggaaggtca
     7141 taccagccac tggccctccg atcgatgctg atgttgaatt gcagctctct tgcctctccc
     7201 gctgggagaa tggcaatgtg ctgcacgccg gaggtcacct gaatggggcc ggccgactgc
     7261 aaagcggctc tgacatccat ggccagagag ctgttcatct cctctctttg ctcttgcagg
     7321 atatcatcgg gggaatcgct gctgcttata ggtatcagct cctcgattct gccctggttg
     7381 gcaatgctta ttctcaggac accgtcgccc ggcgagagtg ctggattggt gactgaggca
     7441 ttgagttgca gatggctgat ggacttgtag tgatcatcgg caaaaaagat gatatcttct
     7501 gcctggccca ggccgaccag gatcagcaga atgcagatga tctt
//
LOCUS       DAOSGP010000039         7528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_8460_length_7528_cov_17.599625, whole genome shotgun sequence.
ACCESSION   DAOSGP010000039 DAOSGP010000000
VERSION     DAOSGP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8460_length_7528_cov_17.599625"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLV49_02120"
     CDS             complement(<1..198)
                     /locus_tag="PLV49_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97667.1"
                     /translation="MKEEEIAYIEGENGTIALAKTKGHFFYLETEQKEIVLFTEPEEL
                     MVASSFGRGEKIRRGLHCTLFQ"
     gene            complement(528..1835)
                     /locus_tag="PLV49_02125"
     CDS             complement(528..1835)
                     /locus_tag="PLV49_02125"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012068054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HOG97668.1"
                     /translation="MATKNIVCPVCGASCDDIQVELEGQGLTVRNACKMGNAKFQEVV
                     SPHRIREPLVRVDGDLRRASWDEAIEMTARILANSERPLLFMGSETSTEAMEVGIHMA
                     EFLGGIADSNSTVCHGPTTMGIQEAGRVGATAGQSKSRADLTVYWGSNPLESMPRHMS
                     RYAVYPRGYWTLRGRFDRTVICVDPRRSITAQNADLHIQLNPNTDYELLSALLTLLHG
                     KRPHQTAEDVTGVSISEMEKMLDMMKSCNFGAIYVGLGIASSYGKHRNAEMAFNLVKE
                     LNSHTKFVIGALRGHCNVAGFNQIASYLYGFPFGLDFARGYPRYNPGEYTAVDVLREK
                     DIDAAFILSADLVSHFPAACSEYLAKIPVACIDIAPCPTTMLSDVVLPGVIDAMESDG
                     TFYRLDDVPIYFQPFTSSPFSFTESNVDTMNQIFERVKRIKGK"
     gene            complement(1839..>2186)
                     /locus_tag="PLV49_02130"
     CDS             complement(1839..>2186)
                     /locus_tag="PLV49_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOG97669.1"
                     /translation="LAKGGSKLTDDYKEECAVCEMNLADFLAMGSPDRVKVTSRDGHS
                     VVVYAYGAEKLSSGQVFMPRAIWSNVVVDPETFSSGSPLYKGSPVKVEPSNDMVLSAE
                     ELTAKLYKLQKRG"
     assembly_gap    2187..2286
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2404..4188)
                     /locus_tag="PLV49_02135"
     CDS             complement(2404..4188)
                     /locus_tag="PLV49_02135"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HOG97670.1"
                     /translation="MAANSSKTMIIKNGYLFDPLNAIDGEVKDIFIREGKVVSSLSGQ
                     EAIDAAVIDARGKTVMPGGVDAHSHVAGTKVNAGRLMRPEDHYKSVRKKTDITHSGSG
                     YTVPSVYKQGYDYAAMGYTTVFEAAMPPLEARHTHEEMRATPILDMGAYMVFGNNWFV
                     MRYLKDGDIEKAAAYVAWMMRTHKAYGIKCVNPAGVENWGWAKNVKGLDEPNIHFEVT
                     SREIIRGLAEVNELLGLPMSLHLHANNLGHPGNWETARDSLMITSSLEPNSKTELEWA
                     QTRESAKRKQTVYLAHAQFSSYGGSSWRDFCSGAPDLAKYINQTENVVIDSGSVPFGP
                     ATTMTGDGPAQHDLYMLTGQKWSNCDIEMECGSGVLSLMYLKSSPVHATMWAIGLEVL
                     LLIDDPWKTIMTTDHPNGGLFSQYPQVIAWLMSKKARDDTAKECHKWAYDKSSLGGVD
                     REMSLYDIAILTRANSARTIGMAHRKGHLGAGADGDVTIYDIDPGNLNVNDPSYIVSR
                     FSRPDFTIKDGQIVARKGEIVYVPEGRRYYCQPHMDEDVEKDMMRDVRDWFKYYTVGF
                     ANYPVPDKYLKNPVPIPVNMPMEAVARR"
     gene            complement(4188..>5276)
                     /locus_tag="PLV49_02140"
     CDS             complement(4188..>5276)
                     /locus_tag="PLV49_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG97671.1"
                     /translation="TKTFLTHSCLNRCPEGRVKMREVLLESREDRAQFYLPDRCIGCG
                     TCVQVCPKGELIIGSVGAVVRGLIDKDFIEKRMSGACAFCALCSRVCPTGALEFRKNG
                     IAEMDNSYLSVAIKPTIVDSEKCVHCGLCCDVCPQACIELEDRHLAEDGSLKVGGRTI
                     IDLERCVHCGWCASVCPTGAISFEKPFAGTFSRDDNVCQACKTCVHTCPANALFNREW
                     KAGERGEKVSHRPDACIYCGACAQACPVRAIVVSKTAIIPVMKGKGAFEKKLALPAPW
                     PTLTSCLETDEIACLGCGNCVIACPVNALSDPYLAAGHLNELERKPLLEVRDGTVKVV
                     DQEVCGSCATCSMICPADAIRLVPREVV"
     assembly_gap    5277..5376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5377..6111
                     /locus_tag="PLV49_02145"
     CDS             <5377..6111
                     /locus_tag="PLV49_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOG97672.1"
                     /translation="GDQMLSDAERERYSRQIRLFAEEGQERLKKKRVFIAGAGGLGSV
                     ISLYMTACGFGRIRIADCDSVDLSNLNRQILHGSLDLGRSKAQSALETLSDINPEVEI
                     EALRERISAESINALVQGCDMIMDALDNFPARYLLNHAALERRIPLFHGAISGFQGQA
                     TTILPGRTACLRCIFPRAPPTQKSPALGSTCGIIGSIQVNEAIKFALGRGELLKNRLL
                     LWDGLSSTLEEVSCEKSMSCMECGGD"
     gene            6203..6652
                     /locus_tag="PLV49_02150"
     CDS             6203..6652
                     /locus_tag="PLV49_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HOG97673.1"
                     /translation="MTEGKKALLVMGCPEVPVQISIVIYLAHKLKKEGWDVTVAGTNA
                     PIKLLKTADPEGYYVQKTADLDWIIQEIVEKKMDFDACFAFMHSDAGMAYGATFSAIS
                     KAVHYAVVFGKNAEALGESIDYPTEKIVAKATHNPGPLKSKIDKVII"
     gene            6649..7041
                     /locus_tag="PLV49_02155"
     CDS             6649..7041
                     /locus_tag="PLV49_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HOG97674.1"
                     /translation="MSCIEQMKYKLYLERTSFKEAREFIEKNSEEVYYVEPGYRIFKD
                     YYIIGVPPVALGIRGDEILFTYVKPCHGTFVMGVDSSEEIARLRDREEEKIKKTLKDG
                     QKKGSEKGKSEAETKASIISYGDVYKNK"
     gene            7083..7361
                     /locus_tag="PLV49_02160"
     CDS             7083..7361
                     /locus_tag="PLV49_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOG97675.1"
                     /translation="MLVKVRAFAQFREILGKEMDMNIPEGSTVLFLLRSIGASSPAFR
                     EQAFSGSGRPNDYILLMINRKRIDPLNDLSIPLNDGDELAIFPPVAGG"
     gene            7368..>7528
                     /locus_tag="PLV49_02165"
     CDS             7368..>7528
                     /locus_tag="PLV49_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOG97676.1"
                     /translation="MFKITAEEISIEEMIQRAKREDSGATVAFLGTVRDDGIQMMEVE
                     AFTEAAEAEL"
BASE COUNT         1610 a         1996 c         2026 g         1696 t
ORIGIN      
        1 ctgaaagagg gtgcagtgca gaccccgcct gatcttctct ccccggccaa aggaagaagc
       61 aaccatcaac tcctctggct cagtgaatag aacgatctct ttttgctccg tctccagata
      121 gaaaaagtgc cctttggtct tagccagagc tatggtgcca ttttcgccct cgatataggc
      181 gatctcttcc tctttcaaag gtatatgcat gacctatgaa tgaaccatta acattccgat
      241 ggacttgtat ggattcggat tccagttcag actgccttta cattccttga cttacaggaa
      301 ggacacatgg gccttcttcc catgccctgg aacttgttat tgcagtccag gcactcataa
      361 tccttggcaa ccagagtctc tacgtctcct aagtccggcc cgccaccgac agtacacatg
      421 ctgccttcac ctcctccttt tccattaggc tatcggatta tttaaaacaa ttggcatgat
      481 aatcatcttt tccccaacct cagttcattc tgatctctcg acccccttta ctttcccttt
      541 atccttttca cccgctcgaa tatctggttc atggtatcga cattgctctc cgtgaaggag
      601 aagggcgagc tggtgaacgg ctggaagtag atcggcacat cgtccagacg gtagaaggtg
      661 ccgtcactct ccatggcatc gatcactccc ggcaggacaa catcagataa catggtggtc
      721 ggacagggag cgatatctat gcaggccacg ggaattttgg ccaggtactc cgagcaggcg
      781 gcaggaaagt gagataccag atccgccgat aggatgaaag cggcatcgat atccttctcc
      841 cgcaagacat ccactgccgt gtactctccc ggattgtagc ggggatagcc gcgagcgaaa
      901 tccaggccga aggggaaacc gtatagatat gatgctatct ggttgaaccc ggccacattg
      961 cagtgccctc tgagagctcc gatcacgaac ttggtgtggc tgttcagctc ctttaccagg
     1021 ttgaaggcca tctctgcatt tcgatgcttt ccgtatgagg aagcaatgcc gaggcccaca
     1081 taaatagccc cgaagttgca gctcttcatc atatccagca tcttctccat ctcggagatg
     1141 gacacaccgg tcacatcttc agccgtttgg tgcggccttt tgccgtgcag gagggtgaga
     1201 agagcactga gcagctcata gtctgtattg ggattgagct gaatatgcag atccgcattc
     1261 tgggcggtta tgctcctcct cgggtcgacg cagatgaccg tcctgtcaaa cctccccctc
     1321 agggtccagt agccccgggg atagacggca taacgggaca tgtgcctggg catggactcc
     1381 aggggattgg agccccagta gaccgtcagg tccgccctcg acttgctctg tccggcggta
     1441 gcccccaccc gtccagcttc ctgaatgccc atggtggtgg gcccgtgaca gacggtggag
     1501 ttcgagtcag ctatgccccc caggaactcc gccatatgaa ttcccacctc catcgcctca
     1561 gtagaggtct cgctgcccat gaaaaggagg ggacgctctg aatttgccag gatcctcgcg
     1621 gtcatctcaa tggcctcatc ccagctcgca cgtctcagat ctccatccac tcttacaagc
     1681 ggctctcgga tcctgtgagg gctcacgacc tcctggaact tggcattgcc catcttgcag
     1741 gcattcctga ccgtcagacc ctgaccctcc agctccacct gaatatcatc acaagaggcc
     1801 ccgcagactg gacagactat attttttgtc gccattatct aaccccgctt ttgcagcttg
     1861 tatagctttg ctgttagctc ctcagccgag aggaccatat catttgaggg ctcgactttc
     1921 accggcgagc ccttgtaaag aggtgagccg gaagagaagg tctccgggtc caccaccaca
     1981 ttggaccaga tggctctggg catgaagacc tggccagaag agagcttctc cgctccataa
     2041 gcataaacta caaccgagtg gccgtccctg ctggttactt taactctatc aggcgaaccc
     2101 atggccagga agtccgccag gttcatctcg cagacggcgc attcctcttt gtagtcatcg
     2161 gtgagcttgc ttcccccttt ggccagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnaaaa gctcctctat ctgctctttt gtctttccag caattaggtc agggcggatg
     2341 acctcagcct ctacggtaat gccaatcttg cccctgggaa caagctttat ttctgccatc
     2401 ttcctacctc ctcgccactg cctccatggg catgttgacc ggtattggga ccgggttttt
     2461 caggtactta tcggggacgg gatagttggc aaagccaact gtatagtatt tgaaccagtc
     2521 ccgaacatcc ctcatcatat ccttttccac atcttcgtcc atatggggct ggcagtaata
     2581 tctccggccc tcgggcacat agacgatctc tcccttccgg gcgacgatct ggccgtcttt
     2641 tattgtgaaa tcgggccggc tgaaccggga gacgatatat gatggatcgt tgacattcag
     2701 attcccagga tcgatatcat agatggtcac atcgccgtct gcgcccgctc ccaggtggcc
     2761 tttgcggtgg gccatgccta tggtcctggc cgagttggct ctggtgagga tggcaatatc
     2821 atagaggctc atctcccggt ccactcctcc caggctggac ttatcataag cccatttgtg
     2881 gcactctttg gcggtatcgt ccctagcttt tttagacatg agccaggcga tcacctgagg
     2941 atactggctg aacaggcctc cgttgggatg gtcggtggtc atgatcgtct tccagggatc
     3001 gtcgatcagc aggagtacct ccaggcctat ggcccacatg gtggcatgga ccgggctgct
     3061 cttgaggtac atcaatgaga ggactcctga gccgcattcc atctctatgt cgcagttgga
     3121 ccacttctgg ccggtcaaca tgtagaggtc atgctgggcc gggccgtcgc cggtcatggt
     3181 ggtggccggg ccgaagggga ctgagccgct gtctatgacc acgttctccg tctggttgat
     3241 gtatttggcc agatcgggag cgccggagca gaagtccctc catgaagatc ctccatagct
     3301 actgaactgg gcgtgagcca ggtagacggt ctgttttctt ttggcgctct cacgtgtctg
     3361 ggcccattcc aattccgtct tgctgttcgg ttccagagag gaggtgatca tcagcgaatc
     3421 tcgggctgtc tcccagttgc caggatggcc caggttgttg gcatgcaggt gaagtgacat
     3481 gggaaggccc agcaattcgt tgacctcagc caggccccgg atgatctccc gggaggtgac
     3541 ctcgaaatgg atgttgggct cgtccaagcc tttcacgttc ttggcccagc cccagttctc
     3601 aacgcccgcg ggattgacgc attttatgcc gtaggccttg tgagttctca tcatccaggc
     3661 cacataggcc gctgccttct cgatgtcgcc atccttcagg tagcgcatga caaaccagtt
     3721 gttgccaaag accatgtagg cgcccatatc caggatgggg gtggccctca tctcctcatg
     3781 ggtatggcgg gcctccagag gtggcatggc cgcctcgaag acggtagtgt agcccatagc
     3841 agcatagtca tacccttgct tgtagacgga tgggacggtg tagccgctgc ccgaatgggt
     3901 gatgtcggtc ttcttgcgga cggatttgta gtgatcctcg gggcgcatga gccggccggc
     3961 gttgaccttg gttcctgcca catgggagtg ggcgtctacc ccgccgggca tgaccgtctt
     4021 tccccgggca tcgatcactg cggcatctat tgcctcctgg ccggagaggg aggagaccac
     4081 cttgccctct cttatgaaaa tgtcttttac ctctccatca attgcattca gaggatcgaa
     4141 gagatatcca ttcttgatga tcatcgtctt ggaagaattt gctgccatca gaccacctct
     4201 ctgggcacca gccggatcgc atctgccggg cagatcatgc tgcaggtagc gcatgagccg
     4261 cagacttcct ggtcgacgac cttgactgtg ccatctctga cctccaggag tggttttcgc
     4321 tccagctcgt tcagatggcc ggcggcaaga tatggatcgg aaagggcatt gaccgggcag
     4381 gcgatgacgc agtttccgca gcccaaacac gcaatctcat ccgtctccag gcaagaggtg
     4441 agagtgggcc agggagcggg aagggcgagc ttcttctcaa aggctccttt gcccttcatc
     4501 accgggatga tagcggtctt ggaaaccaca atcgctcgca ccggacaggc ctgggcacat
     4561 gctccgcagt atatgcaggc atcaggccgg tggctgacct tctctcccct ctcccctgcc
     4621 ttccattccc ggttgaacag ggcattggca ggacaggtgt gaacgcatgt cttgcaggcc
     4681 tggcagacgt tatcgtcacg gctgaaggtc ccggcgaagg gtttttcaaa ggatatagcc
     4741 cccgtcgggc agaccgaggc gcaccagccg caatgcacac agcgctccag gtcaattatc
     4801 gtccggccgc cgaccttgag gctgccgtcc tctgccagat gcctgtcctc tagctcaatg
     4861 caggcctggg ggcagacatc gcagcacagg ccgcagtgaa cgcacttctc gctgtcgaca
     4921 atggtgggct ttatcgcgac gctgagatag gaattatcca tctctgcaat gccattcttt
     4981 ctgaactcca gggcgccagt gggacagacc cgggagcata gcgcacagaa ggcacaggcc
     5041 ccgctcattc tcttctcaat gaagtccttg tcgatcaggc cgcggaccac agctccaacc
     5101 gatcctatga tcagctcccc tttggggcag acctgaacgc aggttccgca gccgatgcac
     5161 ctgtccggca gataaaactg cgctctgtct tctcgggatt ccagcaatac ctctctcatc
     5221 ttaaccctgc cttctgggca tcgatttagg caggaatggg ttaagaatgt tttcgtnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggtg atcagatgct gagcgatgcc
     5401 gaaagggaga ggtactcccg tcagatccgc ctctttgctg aggagggcca ggagaggctg
     5461 aagaaaaaga gagtcttcat agcaggagca ggtggtctgg gctctgtcat ctcattatac
     5521 atgacggcct gtggctttgg caggattcgc atagcggact gtgattcagt tgacttgagc
     5581 aacctcaacc gacagatcct tcatggcagc ctggacctgg gccggagcaa ggcccagtca
     5641 gctctggaga ccctgagcga tatcaacccg gaggtagaga tcgaggccct gcgggagaga
     5701 atctctgcgg agagcatcaa tgctctggtg cagggctgtg acatgatcat ggacgcgttg
     5761 gacaactttc cggccaggta tctgctgaat catgccgcct tagagagaag gattcccctg
     5821 tttcacggcg ccatcagcgg atttcagggg caggcaacga caatattgcc agggaggact
     5881 gcctgtctgc gctgcatctt tccccgcgct ccgccgacac agaagtctcc tgccctgggc
     5941 tcgacttgcg ggatcatcgg ctccattcag gtgaatgaag ccataaagtt cgcccttggc
     6001 agaggcgagc ttctgaaaaa ccgccttctc ctctgggacg gtctctcttc caccctggag
     6061 gaggtttcct gtgagaagag catgagctgc atggaatgcg gaggggattg agagcagcag
     6121 tcccgccgca tggaataatc tattaagctc gactgaggat atctaaatcg aatcaggatt
     6181 atcaagatca agagatgagc atatgaccga aggcaagaag gcgctgttgg tgatgggctg
     6241 cccggaggtg ccggtgcaga tcagcattgt gatctatctc gcccataagc taaagaaaga
     6301 gggctgggat gtcactgtgg cgggaacaaa tgccccaatc aagctgctca agacagcaga
     6361 tcctgagggc tattatgtgc agaagacggc ggacctggac tggatcatac aggagatcgt
     6421 ggagaagaag atggacttcg acgcctgctt tgccttcatg cacagcgatg ccggcatggc
     6481 atatggggcc actttcagcg ccatatccaa agccgtgcat tatgcggtcg tcttcggcaa
     6541 gaatgcagag gcgcttggtg agagcataga ttatccgacg gagaagatcg tggccaaggc
     6601 gacccataat cctgggcctc tgaagagcaa gatagacaag gtgatcattt gagctgcatt
     6661 gagcagatga agtacaaact gtacctggag agaacctcat ttaaagaggc gcgggagttt
     6721 atagagaaga actctgagga ggtttactac gtcgagccag gatacaggat cttcaaggac
     6781 tattacataa ttggcgtgcc gccggtggca ttgggcataa ggggggatga gattcttttc
     6841 acctatgtca aaccctgtca cggcacattc gttatgggcg tcgattcctc tgaggagatc
     6901 gcccggctga gggatagaga ggaggagaag atcaagaaga cgctgaaaga tggtcagaag
     6961 aaaggctcgg aaaagggcaa gtcagaggca gagacaaagg catctattat cagctatggt
     7021 gatgtttata aaaataaatg atcggtgaac ataaaagtca tcgggaacag ggtcgacagc
     7081 aaatgctggt aaaggtcagg gcattcgccc aatttcgaga gatcctggga aaggagatgg
     7141 atatgaatat cccggagggc tcgactgttc ttttcctgct gaggagcatt ggagcatcct
     7201 ctcctgcctt cagggaacag gccttttctg gctctgggag gcccaatgac tatatccttt
     7261 taatgatcaa taggaagagg attgatccat tgaacgatct ctccattcct ctgaacgatg
     7321 gcgatgagct ggccatattt ccgccggtcg ctggaggttg aatagaattg ttcaagatca
     7381 ctgcagagga gatctctata gaggagatga tccagagggc gaagagggag gattccggtg
     7441 ccacggtcgc attcctggga acggtcaggg acgacggcat ccagatgatg gaggtagagg
     7501 cgttcactga ggcggctgaa gccgagct
//
LOCUS       DAOSGP010000040         7123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_9173_length_7123_cov_17.213639, whole genome shotgun sequence.
ACCESSION   DAOSGP010000040 DAOSGP010000000
VERSION     DAOSGP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9173_length_7123_cov_17.213639"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1188)
                     /locus_tag="PLV49_02170"
     CDS             complement(115..1188)
                     /locus_tag="PLV49_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HOG97677.1"
                     /translation="MPADLLAAVVLALAVLLFTLTPLSGLFLRIPIGLLMVLFVPGYV
                     LIAALFPRKGDLDGIERIALSFGLSIAVVPLIGLGLNYTPWGIRLIPVVISIVAFTLL
                     MAAVAYWRRISLSQEERFSLHLRQWIDSLKDEIRAEGERGWVDKALTIILILSIIASI
                     VALVYVVVTPKEGEKFTEFYILGPGGKAYDYPTEVRAGNNSTVIVGVVNHEYRLTNYT
                     MEIQLNNTPAVDILLNDTEENNSLNDATIALSTFEHDRSALIIPIMRMNLSLQHNDTW
                     EQPLTYILDETGDRQKLQFLLYKEGNFSAPYRDLHLWVNVTDMNRRPSKDQGNDGNQD
                     WTQDWQDFPDRLIKFRGNLPPER"
     gene            complement(1235..2053)
                     /locus_tag="PLV49_02175"
     CDS             complement(1235..2053)
                     /locus_tag="PLV49_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97678.1"
                     /translation="MPKKPEWMRNAGRGLALAMAINLMLIFILISPGTSFELSADSVT
                     AGENLIITGTSEPNSENTFISSFTMNLPVTSEKYNYETEVKVPNKPNRFTVTAKNVQN
                     LNAGVKMGIWITKSFESRDGVVHLSAADIPTGRYKLKVSGSAMPGANEVPITIEAETG
                     VLSDSSGSYKLVIDTTGIPEGDYLIKGDGDEKTVRILSKGESSSSASAISEAEKEIAA
                     YKDKIEINLSAAASESAGGKSVNEEVSKEEKATPPAESAESKGVVGWLTDFLGL"
     gene            complement(2135..2860)
                     /locus_tag="PLV49_02180"
     CDS             complement(2135..2860)
                     /locus_tag="PLV49_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97679.1"
                     /translation="MLRVEKAVACIISLSILALGQIAWAQDQAIEPMTLVPGADYSEM
                     DLTNLVLGSKEMTISGSGEGELQITLSFTTTVPVVSGSYNYDISGFEMPPFSTPVQLK
                     SSGAGYDLSVPGILLPSKPNDVTLTAGPVKSMNVGFMRLDGNANRPWTSRQFQADQSN
                     QAVARSDLLYPNPSGVYRVRIFGDAPTDATTVQLTLTAEKRVVINGPFDIVANTAGFP
                     QGRYSLRARAISGSFEQIEFGFA"
     gene            complement(3110..4120)
                     /locus_tag="PLV49_02185"
     CDS             complement(3110..4120)
                     /locus_tag="PLV49_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97680.1"
                     /translation="MDRASVGTALMSNATAAAEIGDYAQINITSGKRPENDETFWSLE
                     GYDRIQLPRMGRILQDNQTSQDLLLPEELAVYSQGMPLQGKIEFSREQVPFKIPSEPG
                     INVCIAPLSLAVYLAGDLANAQLSEEAICTEAVVMDSAENDSAAKKANLSFSIASDRT
                     AERRSGLYALYALDGNRSSIAPAQAFILTEGEAVLQMEDSIPSENEFIKINLSTGTED
                     NRTKIFAAAIMPRKEYDNATMRLVANESKAWPVVALSIGTRSTEIEINPANAQDQLMG
                     MISLLPDNSAISMQEATLSAVELIFLTDGSWVKGEYVIVCAIYSPEVGLQGLKQKIVE
                     VI"
     assembly_gap    4252..4351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4352..5118)
                     /locus_tag="PLV49_02190"
     CDS             complement(<4352..5118)
                     /locus_tag="PLV49_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOG97681.1"
                     /translation="MRSVSIIGVGCTKFGERWDSSLRDMIAEAGVLAIEDAEITGEQI
                     DALYVGNMSGGRFIEQEHIGALIADCAGLSRLHVPSTRVEAACASGGLALRQAVMAVA
                     SGYCDIVIAAGVEKMTDVSSGTAADALAAAADREWECFFGATFPALYAMMAKMHMRRY
                     GTTHDQMAQVAVKNHHHACMNPIAQYQMEIGIDDVNRSSMVADPLHVLDCSPISDGAS
                     VVVLAPTETARRFSDTPIKIVGCAQASDTLALHDRRDL"
     gene            complement(5115..6152)
                     /locus_tag="PLV49_02195"
     CDS             complement(5115..6152)
                     /locus_tag="PLV49_02195"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOG97682.1"
                     /translation="MVSGIVSYGVYIPRFRIRADEIARVWGGGDDISLSLRVFEKSVP
                     DLDEDAVTIAVEAARNALDRADVDPKRIGAIYTGSESHPYAVKPTGTIVGQAVCTSSS
                     FTTADFEFACKAGTAAIQACLGLVGSDMIDLGLAIGADVSQGAPGDMLEYTAAAGGAA
                     YLIGKRDLAAQIEGFYSFTTDTPDFWRREGMPYPEHSGRFTGEPAYFKHVASAATGLM
                     ERMKTRPEDYDYAVFHQPNGKFPVRVAKMLGFSKKQIEPGLVVPMIGNTYSASCLIGL
                     AATLDIARPGERIFVTSFGSGAGSDAFSIKVLDRIEDIRCNAPSVRDYIGDAEYLDYA
                     MYAKHKGKLRI"
     gene            complement(6159..6878)
                     /locus_tag="PLV49_02200"
     CDS             complement(6159..6878)
                     /locus_tag="PLV49_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG97683.1"
                     /translation="MNSGGEISNARGLLAEKIAGEITLSQNPGETLRKWRRNFGITQT
                     DLSNHLKISPSVISDYESGRRKSPGIMIVSKIIDALLRLDESRGCNVLRAYEDMWGDI
                     LGLDSICSVCEYQEPLPLNDIAKKLDAEVVYGRTDITIWGYTLIDSLKAIVEMLPDQF
                     QKIYGRSTARALIFTGVHTGRSSMVAVRVSNLKPGAVVLQGMEPVDVDPIAVRIAEVE
                     NIPLLTTHFSVDEITSALSKG"
     gene            complement(6984..7081)
                     /locus_tag="PLV49_02205"
     tRNA            complement(join(6984..7018,7045..7081))
                     /locus_tag="PLV49_02205"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7046..7048),aa:Gly,seq:tcc)
BASE COUNT         1548 a         1935 c         1759 g         1781 t
ORIGIN      
        1 gatatgggct gctttgagag ctatatggag tttcgcgaag aggtagaaaa gatcaaaatc
       61 aatcagagga accgcccaga tctgctgagt ggcaaaaaag gtgcatccgg agacctacct
      121 ctccggaggg agattgcccc tgaatttgat taaacgatct ggaaaatcct gccaatcttg
      181 ggtccaatcc tgattcccat cattgccctg atcctttgat ggccttctgt tcatatcggt
      241 cacatttacc cagagatgca aatccctata aggggcggag aaatttccct ctttgtagag
      301 gaggaactgt agcttctgcc ggtcccctgt ttcatccagg atataggtta agggctgctc
      361 ccaggtatcg ttgtgctgca ggctgaggtt cattctcatt atggggatga tgagagctga
      421 tcggtcgtgt tcaaatgtgc tcaatgcaat ggtggcatca tttaggctgt tgttctcttc
      481 ggtgtcattc agaagaatat ccactgcggg ggtattgttc agctgaatct ccatagtgta
      541 gttggtgagc ctgtactcat ggttgaccac ccctacgata actgtgctgt tgtttcctgc
      601 tctgacctcc gtcggatagt cataggcctt ccctcctggg cccaggatat agaactccgt
      661 gaacttctct ccctccttgg gagtgaccac cacataaaca agggcgacga tggaggcgat
      721 tatggatagt atcagaatta tggttaaggc cttgtccacc caacccctct ccccctctgc
      781 tcggatctca tctttcagtg agtctatcca ctggcgaaga tgaagggaga acctctcctc
      841 ctgggaaaga gagatacgtc gccagtaggc aactgcagcc atcagcaggg taaaggccac
      901 aatggatata acaaccggta tcagcctgat tccccagggg gtgtagttga ggcccagacc
      961 gatcaggggg acaacggcga tgctcaagcc gaagctaaga gctatcctct caatcccatc
     1021 aaggtctccc tttcttggaa agagggcggc gatgagcaca taccctggca caaagagcac
     1081 catcaaaagg ccgatgggga ttcgcaggaa caggccgctc aaaggggtga gggtgaagag
     1141 cagaaccgcc agggcaagca ccaccgctgc caggagatct gccggcatct gctctagatt
     1201 cctctccatc attagtttcc cctgtgtccg ctgcctatag ccctaaaaaa tctgtcagcc
     1261 agccaactac tcccttgctc tcggcggatt cggctggcgg ggtggctttc tcctctttgc
     1321 tcacctcctc gttgaccgat tttccgcctg cggattcgct tgcagctgcg ctgagattga
     1381 tctcaatttt atctttatat gctgctatct ccttctcagc ctcactgatg gctgaagcag
     1441 atgagctgga ttctcctttg ctcaatatgc gcacggtctt ctcatcgcca tctcccttga
     1501 tcaggtaatc gccctccggt atgccggtgg tgtcgatgac cagcttgtag cttccactgg
     1561 agtcagacaa gacccccgtc tcggcctcaa tggttatggg aacctcattt gctcccggca
     1621 tagcgctgcc ggataccttg agcttgtacc ttccagtggg gatatccgct gcagagagat
     1681 gcactacccc gtcgcgtgat tcgaagctct tggtgatcca gattcccatc ttcactcctg
     1741 cattgaggtt ctgtacattt ttcgccgtaa ccgtaaaacg gtttggctta ttggggactt
     1801 tgacctcggt ctcatagttg tacttctctg aggtcacagg caggttcatg gtgaagctgg
     1861 agatgaacgt gttctcgctg ttcggctcgc tcgtcccggt tatgatcaaa ttctctccgg
     1921 cggtaacgct gtcagcagac agctcaaatg aggttcctgg cgatatcaat atgaagataa
     1981 gcatcaaatt gatcgccatg gcgagggcca gtcccctgcc agcattcctc atccattctg
     2041 gtttctttgg cataatctct tcctccagtc tcagcattct cctgatcgct ccagcaattc
     2101 catctgcata tatttattgt tgatgacgat gggtctaggc aaaaccgaac tcaatttgct
     2161 caaatgatcc acttatggcc ctggcgcgga ggctgtatct gccttgcgga aatcctgcgg
     2221 tgttggcaac aatatcaaat ggtccattga tgacgaccct tttctctgca gtgagggtaa
     2281 gctggaccgt ggtcgcatct gttggagcgt ccccaaatat cctcaccctg tatactccac
     2341 ttggatttgg atagaggaga tcgcttctgg ctacggcctg atttgattgg tccgcctgga
     2401 attggcggct tgtccagggt cggtttgcat ttccatccag ccgcataaag ccgacattca
     2461 tgctcttaac cggaccggcg gtcagggtga cgtcatttgg cttgctggga aggagaatcc
     2521 ccggcactga taaatcgtat cctgcgccgc tgctctttaa ctggactggt gtagaaaacg
     2581 gtggcatttc gaatccggaa atgtcatagt tgtagctgcc cgaaaccaca ggcaccgtcg
     2641 tggtgaagga aagggttatt tgaagctcac cttctcccga tccggagatg gtcatctctt
     2701 tgctccctaa gaccaaattt gttaaatcca tttcactata atccgctccc ggaaccaatg
     2761 tcatcggttc tatggcctga tcttgcgccc aggcaatctg gcctagtgcc agaatgctca
     2821 agctgattat acaggctact gccttctcta ctcttaacat atgcttggtc acctctcgag
     2881 atattcagga tctattccct attcatatta tttaattaat acatttttcc gtctctctct
     2941 cttcaggatt gtccttgatt cttttggaga ttgttcttaa tgctcaattc tattgggtat
     3001 gatatcagat aaaaacctca cgccataact gctcagaaca atttatttca tcattctgaa
     3061 aaatggagga attatgtcac gataaaaatt aattaagggt gcaggcgggc taaatcacct
     3121 caacaatttt ctgcttcaat ccctgcagcc caacctccgg ggaatagatg gcgcatacga
     3181 tcacatactc ccccttcacc catgatccat cagtcagaaa gatgagctca accgcggata
     3241 aagtggcctc ctgcatgctg atggcgctat tgtccggcag aagggagatc atacccataa
     3301 gctggtcttg agcattggca ggattgatct cgatctcagt ggatctggtg cctatggata
     3361 gagcgacaac cggccaggcc tttgattcat tcgcgaccag cctcattgtg gcattatcat
     3421 actctttgcg cggcataata gctgctgcga atatcttggt ccggttgtct tcagtccctg
     3481 tgctcagatt gatcttaatg aattcattct ctgaaggaat gctatcttcc atttgcagga
     3541 ccgcctctcc ttccgttaag atgaaggcct gagcgggggc aatagacgat cgatttccat
     3601 caagggcata tagcgcataa aggccgcttc tcctctcggc cgttctatct gaagcgatgc
     3661 tgaaagatag attggccttc ttcgctgcgc tgtcattctc cgcgctatcc attaccacgg
     3721 cctctgtgca tatcgcttcc tcagataact gtgcattggc aagatctcct gccagatata
     3781 ccgccaggct gagaggagct atgcagacgt tgattcccgg ctcggaaggg atcttgaatg
     3841 ggacttgttc tctggagaac tcgattttcc cctgcaatgg cattccctga gaatagacgg
     3901 cgagctcctc tggcagtagc aggtcctgag aggtctggtt atcttgcagg atgcggccca
     3961 ttctgggcaa ctgaatccga tcgtatccct ccaggctcca gaaggtttca tcattctcag
     4021 gtcttttccc tgatgtgatg tttatctgtg catagtcccc tatctctgcg gctgctgtcg
     4081 cattgctcat taatgctgtg cccactgatg ctctgtccaa ttgggattca ttcgttgcag
     4141 tgcaattcat tcccaaggaa ctgatgagaa gcataaatag gcatagaaaa agcgatatca
     4201 tcctcatagt tcctaataac atttataaag tatttatgca cttccctctg annnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naggtcgcgg cggtcgtgca gggccagggt
     4381 atcgctggcc tgagcacagc ccacgatctt gataggagtg tcggagaacc tgcgagctgt
     4441 ctcggtggga gccagaacca ccaccgatgc accatcggat atgggagagc agtccagcac
     4501 atgcagggga tcggccacca tagaggatct gttcacatca tcaatgccga tctccatctg
     4561 atactgggcg atgggattca tgcaggcatg atggtgattt ttcactgcca cctgggccat
     4621 ctggtcgtgg gttgtgccgt agcggcgcat atgcatcttg gccatcatgg catagagcgc
     4681 tggaaatgtg gcgccaaaga agcattccca ctccctgtct gctgctgctg ccagggcgtc
     4741 ggcggcggtg cctgaggaga cgtcggtcat cttctcgacc ccggcggcga tgacgatatc
     4801 gcagtagccg ctggccaccg ccatgaccgc ctgacggagg gcaagacctc cggaggcgca
     4861 ggcggcctct acccgggtgg aggggacatg cagccgggag agaccggcgc agtcggcgat
     4921 cagggctccg atatgctcct gctctatgaa ccgcccgccg ctcatgttgc ccacatagag
     4981 ggcgtctatc tgctcaccgg tgatctctgc gtcctcaatc gccaggaccc ctgcctcggc
     5041 gatcatgtcg cgcagggaag agtcccatct ctccccgaac ttggtgcaac ctacaccaat
     5101 aatagataca gacctcatat tctcagcttg cccttgtgct tggcgtacat tgcataatca
     5161 agatactcag catcgccgat atagtcgcga acgctcggag cattgcagcg gatgtcctca
     5221 attctgtcca ataccttgat gctgaaggca tcgctgcccg caccggagcc aaagctggtg
     5281 acgaagatcc tctctcccgg ccgggcgatg tccaaggtgg cggccaggcc gatgaggcag
     5341 gatgcagagt aagtgttccc tatcatgggc acgaccagcc ccggctcgat ctgctttttg
     5401 gagaagccca gcatcttggc cactcgcacc gggaacttgc cattgggctg gtggaagact
     5461 gcatagtcgt agtcctccgg cctggttttc atcctctcca tcaatccagt ggccgcgctg
     5521 gcgacatgct tgaaataggc gggctcccct gtgaacctgc cgctatgctc tgggtagggc
     5581 attccctctc ttctccagaa gtccggggta tcggtggtga aggaataaaa gccctcgatc
     5641 tgggccgcca ggtccctctt tccgatcaaa taagccgccc ctccggcagc tgcggtgtac
     5701 tccagcatat cacctggcgc tccctggctg acgtctgcgc cgatggccaa acccagatcg
     5761 atcatatcgg agcctaccag gcccaggcag gcctgaatcg ccgcggtccc cgccttgcag
     5821 gcgaactcaa aatccgcagt ggtgaaggag gatgaggtgc ataccgcctg gccgacaatg
     5881 gtccccgtag gcttgaccgc atatggatgg ctctccgagc ccgtatagat ggcccctatt
     5941 cgcttcggat ccacgtcggc gcgatccaga gcgttccttg ctgcctccac cgctatggtc
     6001 actgcgtcct catcaagatc aggcacggat ttctcgaaga ccctcaggct caaagagatg
     6061 tcatcgccac ctccccacac cctggctatc tcatctgctc gtatcctgaa tcggggaata
     6121 tagaccccat agctgacaat acccgagacc aatggatttc accctttgct cagagcactg
     6181 gtgatctcat ccacggaaaa atgtgtcgtc aaaagaggaa tattttcaac ctctgcgatc
     6241 ctgaccgcga ttgggtccac atctactggc tccattcctt gcaggaccac tgcccctggt
     6301 tttagattgc taaccctcac cgcaaccatg ctcgatcggc cggtatgtac gccggtgaat
     6361 atcagagccc tggcggtgct ccggccatag atcttctgga actggtcggg gagcatctcc
     6421 acaatggcct tcaggctgtc tatcagcgta taaccccaga tggtgatatc cgtccggccg
     6481 tagacgacct cagcatcaag cttcttggcg atatcgttca gaggaagggg ctcttgatac
     6541 tcgcagacgc tgcagatgga gtccaggccc aggatatccc cccacatatc ctcataggcg
     6601 cgcaggacat tgcagccccg gctctcatcc agcctgagca gggcatcgat tatcttgctg
     6661 actatcatta tgcctggaga cttcctcctg ccgctctcat agtcggatat taccgaagga
     6721 gatattttca gatgattgga gaggtcggtc tgggttatgc cgaagttgcg ccgccacttt
     6781 ctcaatgtct cgccaggatt ttgggataag gtgatctcgc ctgcaatctt ctctgccagc
     6841 aatcctcgtg cattggagat ctccccacca gaattcatga aatactaact ggtgaaacta
     6901 ggatataaat gtagcgaaaa atattcggca tttaacgtat tggccaccag aataggcaaa
     6961 ttcagaagaa tgaggccgaa gagtgcgtcg accgggattc gaacccgggt tgtaggcttg
     7021 cttcgcagat tctattgatc tgcctggaag gcccaagtca taaccactag accatcgacg
     7081 cccgcccatc tagtctcaca acttgaactt aactgtttcg atc
//
LOCUS       DAOSGP010000041         7121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_9175_length_7121_cov_18.141947, whole genome shotgun sequence.
ACCESSION   DAOSGP010000041 DAOSGP010000000
VERSION     DAOSGP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9175_length_7121_cov_18.141947"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PLV49_02210"
     CDS             <1..1086
                     /locus_tag="PLV49_02210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG97684.1"
                     /translation="GASVMLLDRNLGRLHHDANTLFEGMAAVSGIKIDDCLLKKELEG
                     MRILSPSGKGVTIPAKGYFIDREAFDRHYLHLAEKAGVTLLSDEVRSLHLEGNGRTVG
                     LKGVQIHARVVIDASGVQSGLSEKAGLKPMRHPEDIAWAVEADIEYPYLGEEMFFQYW
                     VGSMAPGWKATFSPAGGDRATIGVFVRGHGQDVQPFFDRFSKLFKAQKASQYKNIDRM
                     KVLSVRRGGDPICVMPGEMVADSLMVAGGAAGQSGLAYSMRAGTICGTVAAEAVLKDD
                     VSHRSLSEYVRRWKREFYWQYRMGRSSLQTLGQMKDEEIERLIYGLSGKGLVSNGSFF
                     KKAVYAGALVTMIRPQTPYDLLVNLIR"
     assembly_gap    1089..1188
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1189..1686
                     /locus_tag="PLV49_02215"
     CDS             <1189..1686
                     /locus_tag="PLV49_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97685.1"
                     /translation="VLKTRNTPLTEADFRIHLNINWCRGVLFNVVALKNSIAVVEYDA
                     ILWSEDSIMFIEYKDSPAAYKDLSSRRVQQMNSFAKNIARGLGFKSFNFVVVVKGLEE
                     STSKGGVVVMPLVELGSYQPNFVSSIAELEYLDKMIAKYSRAGEAQFALDLEKLRKIF
                     EIEQA"
     gene            1705..2553
                     /locus_tag="PLV49_02220"
     CDS             1705..2553
                     /locus_tag="PLV49_02220"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOG97686.1"
                     /translation="MKGYGWSNGAASIINAVATWKGSAFAIELVTRAVVELDHSSGIK
                     GDVPGVDTRLIERCVVRVLDYLGLEYGGRVRTSSEIPIASGLKSSSAAANAVILATLD
                     ALDVDMDLVSAAKIGVEAAREVGVTITGALDDALASMLGGVVVTDNREMSLLRRDELN
                     CSVMLLVPDQRIFSRDTDISRSRIIAPMADLAFDLAMQADYGRAMTLNGLAYCAALGL
                     SSEPLILALEKGARGASLSGTGPSYAALIDESKMDGLEESWRALGGRVIRTKANNRCA
                     SRGQGF"
     gene            2550..2834
                     /locus_tag="PLV49_02225"
     CDS             2550..2834
                     /locus_tag="PLV49_02225"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOG97687.1"
                     /translation="MTDLNKYRSDIEEVDQSIIELIDKRIGITKRIFEVKRREGRPIR
                     DPEQEGRVLRRAMDLATEKNLDAGAVKNIYEILIRMSTQKQQELQGENQS"
     gene            2900..3289
                     /gene="purE"
                     /locus_tag="PLV49_02230"
     CDS             2900..3289
                     /gene="purE"
                     /locus_tag="PLV49_02230"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOG97688.1"
                     /translation="MVDIAVISGSKSDQLIVDRILGRLKDEGVSFEHRVLSAHRNPKE
                     LEEYVSGTDAHVFIAVAGLSAALPGVIASQTHRPVIGVPVSAKLGGLDSLLSIVQMPP
                     GVPVACVGIDNGENAALLAIRILESGR"
     gene            3336..3767
                     /locus_tag="PLV49_02235"
     CDS             3336..3767
                     /locus_tag="PLV49_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG97689.1"
                     /translation="MYKRILIATDGSEKSKAAAKEGMELAKALGAQVIALNVVNEVVI
                     ASAVRQLGSDRKDVEARLKAAGEKAVEELKSMGASAGINIDTVVRIGAPATAIIDTAG
                     AEKADLIVVGSHGESGATKLLIGSVAQKVLYWATIPVLVVR"
     gene            complement(3985..5037)
                     /locus_tag="PLV49_02240"
     CDS             complement(3985..5037)
                     /locus_tag="PLV49_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOG97690.1"
                     /translation="MSDIKSLLEKLSNAHGISGWEGSVQKIVQEEISPYVDEVRVDKL
                     GNLIATKKGDRPSIMIDAHADEIGLMVKQIDEKGFIRFIRIGGWFDQTLLNQRVIIHT
                     SSGPVVGVIGSKPPHVMKDEDRKKVVEARDMFIDIGCSNQVEVEELGILPGTPISIDR
                     EFVPMHGDTVTGKAFDNRAGLVMMIEALKRTKSKSTIYAVAAVQEEVGLKGAKVAAFG
                     LDPDIAIASDVTIPGDHPGIEKKDAPIEMGKGPVLVVADGSGRGLIATPQVIEWLVGT
                     AKEFEIPVQLEASDGGTTDATSIYLTRSGIPTGVISVATRYIHSPVEVLNLNDIERAA
                     DLMARSLETASRYFKE"
     gene            5105..6376
                     /locus_tag="PLV49_02245"
     CDS             5105..6376
                     /locus_tag="PLV49_02245"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HOG97691.1"
                     /translation="MTSRIPGFYKLPPNERLKAVAEQVAVGSEEISQVERGLVLDQAE
                     KMVENVIGMFQVPLGIATNFVIDGQEVLIPMATEEPSVIAAASNGARMARGGGGFLTS
                     STGPVMRAQIQATGIDDPFAARQAILLHKDELMAMANELDPMLVKYGGGVKDIEVYVI
                     DSRLGPMVVTHLIVDCRDAMGANAVNTMAEALAPRIEQITGGRVYLRIISNLADLRLA
                     RAKAVFRAEDIGGPEVVDGIILAAELAVVDSYRAATHNKGIMNGVTAVVLATGNDTRA
                     VEAGAHSYASRTGRYMSLTRYERNRDGDLVGTIELPVAVGLVGGATRVHPVAKTAVKI
                     LAVKSADDLSRKIAAVGLCQNFAALRALASEGIQRGHMSLHARNVAVQAGAKCDLIEL
                     VAARMAAERRINVDRAAELIRELEDRGQEGV"
     gene            6373..>7121
                     /gene="pheA"
                     /locus_tag="PLV49_02250"
     CDS             6373..>7121
                     /gene="pheA"
                     /locus_tag="PLV49_02250"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOG97692.1"
                     /translation="MKTRIGVLGPEGTYSEKAALIWSEGMPSTVLVYFKDFDGVLQGV
                     EEGELDYGIVPLENSLEGAVTVVNDLLLRLNVVIVGEVNVPVRHCLVGQGEGEIKIIL
                     SHPQALAQCRQFIREHYPGAEIRTTGSTSHAARLAQEFGEMAAIADAGAAERYGLRVL
                     ARDVQDVRENVTRFIVAGRQIPGATGKDKTSLIIHLAKDRPGALYSILQEFATRSINL
                     TRIESRPSRLGLGDYYFYIDLEGHQDNSVVL"
BASE COUNT         1579 a         1784 c         2110 g         1548 t
ORIGIN      
        1 ggggccagcg ttatgctgct ggacagaaac ctcggccgcc tgcaccatga tgccaacacc
       61 ctctttgagg gaatggcagc agtctctggg atcaagatcg atgattgcct gctgaaaaag
      121 gagctagagg gcatgcgcat tctcagcccc tcgggcaagg gggtcaccat tcctgccaaa
      181 ggctacttca tagaccggga ggcctttgac cgccactatc tccatctggc agagaaggca
      241 ggagtcaccc ttctctccga tgaggtcaga agcctccacc tggaaggcaa tggaaggacg
      301 gtcggcctga agggagtcca gattcatgcg cgagtggtga tagatgcttc tggggtccag
      361 tccggcctct cggagaaggc aggcctcaag cccatgcgcc acccagagga tatagcctgg
      421 gcagtggaag cagatataga atatccctat ctgggcgagg agatgttctt ccagtactgg
      481 gtgggaagca tggcaccggg ctggaaggcc acattctcgc ccgccggagg tgatcgggct
      541 acaatagggg tgttcgtgcg cggccacggc caagatgtgc agccattctt cgatagattc
      601 agcaagctct tcaaggccca aaaggcttcc cagtacaaaa atatcgatcg aatgaaggtg
      661 ctatccgtca ggagaggcgg cgatcccatc tgcgtcatgc caggagagat ggtggccgac
      721 tccctgatgg tcgctggcgg cgcagccggc cagagcggcc tggcctacag catgcgagcc
      781 ggcaccatct gcggcacagt ggcggcagaa gccgtcttga aggatgatgt atctcatcgc
      841 tcgctctctg aatatgtgcg gcggtggaag cgggagttct actggcagta ccgcatgggc
      901 cggtcatctc ttcagaccct cggccagatg aaggatgagg agatagagag gctgatttat
      961 ggcctctccg ggaaggggct cgtctccaat ggatcattct tcaaaaaagc ggtctatgcc
     1021 ggggcgctcg tcacaatgat acgccctcag accccctatg acctgcttgt gaacctcata
     1081 cgatagtgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tctgaagacc
     1201 aggaacactc ctctgaccga ggcggacttc aggattcacc ttaacataaa ctggtgtcgc
     1261 ggagtccttt tcaacgtggt ggcattgaaa aactcaatcg ctgtggtgga gtatgatgct
     1321 attctatggt cggaagactc cataatgttc attgagtaca aggattcgcc cgccgcctat
     1381 aaggatctct cttcccgaag ggtacagcag atgaacagct tcgccaagaa catcgcccgt
     1441 ggcctgggat tcaagagctt taatttcgtg gtggtggtaa agggtctgga ggaatcgacg
     1501 agcaagggtg gagtggtggt catgcccctg gttgagctgg gaagctacca gccgaacttc
     1561 gtctcatcga tcgccgagct ggagtatctt gataagatga ttgccaagta ctcacgggcg
     1621 ggtgaggcgc agtttgcttt ggatctggaa aagctcagga agatcttcga gatagagcag
     1681 gcctgaggat tggagatgat gaatatgaaa ggatacggat ggtcgaacgg tgctgcctcc
     1741 attataaatg ccgtggcaac ctggaagggc tcggcctttg ccatcgagct tgtaacccgg
     1801 gccgtggttg agctggacca ctcttcagga atcaagggag atgtcccggg ggtagatacc
     1861 cgcttgattg agcgctgtgt ggtgagggtc ttagactatc tgggccttga atacgggggc
     1921 cgggtgagaa catccagcga gattcccata gctagcggcc tgaagagcag cagcgcagcc
     1981 gccaatgccg tcatcctggc aacacttgat gcacttgacg tggatatgga tctggtgagt
     2041 gctgccaaga tcggggttga ggcggcaagg gaggttggag tcaccataac cggcgccctg
     2101 gatgacgctc tcgcctcgat gctcggcggg gtggtggtca cagacaatcg cgaaatgtcc
     2161 ctccttcgca gggatgagct caactgcagt gttatgcttc tggttcccga tcagaggatt
     2221 ttctcccggg atactgatat ctctcgatcc aggatcattg ctcctatggc ggatctggcc
     2281 ttcgatctgg ccatgcaagc cgactatggc cgggctatga ccctgaatgg gctggcctat
     2341 tgcgccgctt tgggcctttc gtctgaacct ctgatcctgg cgctggagaa gggggcacgg
     2401 ggcgcaagcc tctcgggaac tggtccctct tatgctgctc tgatcgatga gagcaagatg
     2461 gatggccttg aagagtcctg gagggcactg ggcggaagag tgataagaac gaaagcaaat
     2521 aacaggtgcg catccagggg gcaaggattt tgacggatct aaataaatac aggagtgata
     2581 tcgaggaggt cgaccaaagc atcatagagc tcatcgataa acgaatcgga atcaccaaaa
     2641 ggatcttcga ggtcaagagg agagagggaa ggccgatccg cgaccctgag caggaaggcc
     2701 gagtcttgag acgggcgatg gacctggcca cggagaagaa tctggatgct ggtgccgtaa
     2761 aaaatatcta cgagattctg atccgtatga gcacccaaaa gcagcaagag cttcagggcg
     2821 agaatcagag ctgagctctc tatgaaaccc ttgatgtgat ggaaggattt ttggccaaaa
     2881 ttgatctctt ggagcagcca tggttgatat tgctgttatt tcggggtcca aatcagatca
     2941 gctgattgtg gacaggattt tgggcaggct gaaggatgag ggcgtctcct ttgagcatcg
     3001 agtgctttcc gcacacagaa atcccaaaga gctggaggag tatgtatctg gaaccgacgc
     3061 ccacgtcttc atcgccgttg ccggcctctc tgctgccctg ccgggagtca tcgcctccca
     3121 gactcatcgc ccagtgatag gggttccagt tagcgcgaag ctgggtggcc tggattcatt
     3181 gctctccatt gttcagatgc ctccaggcgt tcccgtggca tgtgttggca tcgacaatgg
     3241 tgagaatgcg gcgttgctgg ctattagaat tctggaatct ggaagatagc tatttataca
     3301 atgtccgtga gataacggct caaaggtggg ataggatgta caagcggatt ttgatcgcca
     3361 cagatggctc agagaaatct aaagcagccg ctaaggaggg catggagctc gcaaaggcgc
     3421 tgggagcaca ggtcatagcg ctcaatgttg tcaatgaagt cgttattgca agcgccgttc
     3481 gccaacttgg atcagataga aaggatgtcg aggccaggct gaaggctgct ggagagaagg
     3541 cagtagaaga actcaaatcc atgggtgcct ctgccggtat aaatattgac accgtggtga
     3601 gaatcggcgc acccgcaaca gcaatcatag ataccgcagg ggcagagaaa gccgacctga
     3661 tcgtcgttgg aagccacggg gagtccggtg ccactaagct attgatcgga agcgttgcac
     3721 agaaggttct ttattgggcc acgattcctg tcctggtagt gagatgatct gaatggaagc
     3781 tctagtattg ggaactccct tgctctatgc ctcctttgcc attgtattcg tggtgggtgt
     3841 agtcctgggc tactttgtga ttagcttgat cggctcaagc tgaaaggtcg aggaaggtca
     3901 ccggatcact ccggtgaaaa ctttttcttt ttgaaatttt aatcaaaaaa taaaaaagga
     3961 ttgagccgat ttaacgacaa cgcctcattc tttgaaatat ctggatgccg tctccaggct
     4021 tctggccata agatctgctg cccgttctat atcatttaaa ttcagcacct ctactggcga
     4081 gtggatatag cgcgtggcga cgctgatcac gccggtggga atcccggatc gagttaggta
     4141 aatggaggtg gcatcagtcg tcccgccgtc cgaggcttcg agctgcaccg gaatctcaaa
     4201 ctccttggcc gtccccacca gccactcaat cacctgcgga gtagcgatca gtcctcttcc
     4261 cgagccgtcg gccacgacca gtaccgggcc tttgcccatc tctatgggcg catccttctt
     4321 ctcaatcccc ggatggtctc cgggtatggt cacatctgag gcgatggcga tatccgggtc
     4381 caggccgaaa gcggcgacct ttgctccctt caatcccacc tcctcctgca ctgcggccac
     4441 ggcatagatg gtcgattttg atttggtcct cttcagagcc tcgatcatca taaccaggcc
     4501 ggcgcggttg tcgaaggcct tgccagttac ggtgtctcca tgcatcggta cgaactctcg
     4561 atctatggat attggcgtgc ccggcaggat tcccagctcc tctacctcca cctggtttga
     4621 gcagccgata tcgatgaaca tatctctggc ctctaccacc ttctttctgt cctcatcctt
     4681 cataacatga ggaggctttg agccgatcac gcccaccacc gggccggagc tggtatggat
     4741 gatgactctc tgatttagca gggtctggtc aaaccagccg ccaatgcgaa tgaagcggat
     4801 gaaacccttc tcatctatct gttttaccat cagaccgatc tcatccgcat gggcatcgat
     4861 cataatcgat gggcggtctc cctttttcgt ggcaatgaga ttgccaagct tgtccactct
     4921 cacctcatca acgtatggag atatctcttc ctgtacgatc ttctgcaccg acccttccca
     4981 tccggatatg ccgtgggcgt tgctcagctt ttccagcaat gatttgatgt cgctcattaa
     5041 ctccactcgt gattatggga ataaagtttt tcccttctca gtgccatcca gatatctgat
     5101 aacgatgacc tccagaatac caggattcta caagcttcct ccaaatgaga ggctcaaggc
     5161 tgtggcagag caggtggcag ttggatcaga ggagatctct caggttgaga gaggtctggt
     5221 ccttgatcag gctgagaaga tggtggagaa tgtgatcgga atgttccagg tgcccttggg
     5281 catagccacc aactttgtaa tcgatggcca ggaggtcctc ataccaatgg ccactgagga
     5341 gccctcggtg atcgccgccg cgagcaacgg agcccgcatg gcccgaggag ggggggggtt
     5401 tttaacctcc agcaccgggc cggttatgcg cgcccagatc caggccacgg gaattgatga
     5461 tccctttgcc gcccgccagg ccatcctcct ccacaaggat gagctgatgg cgatggcaaa
     5521 tgagttggat cccatgctcg tcaagtacgg tggcggggtt aaggacatcg aggtttatgt
     5581 catcgactcc cggctggggc caatggtcgt cacccatctc atcgtcgact gccgggatgc
     5641 catgggggcg aacgcggtca acaccatggc cgaggctctg gcccccagga tagagcagat
     5701 aaccgggggc cgggtctatc tcaggatcat ctccaatctg gctgatctcc gtttagccag
     5761 ggctaaagcg gtattcaggg cggaggatat cggcggcccg gaggtggtgg atggcatcat
     5821 cctggcagca gagctggcgg tggtcgattc ctatcgagca gcaacccaca ataagggaat
     5881 catgaacggg gtgacggctg tggttttagc cacaggaaac gatacccggg cagtggaggc
     5941 gggagctcat tcctatgcct cccgaactgg ccgctacatg tctttaacca ggtatgagag
     6001 gaacagggat ggcgatttag tgggaacgat tgagcttcct gttgcggtgg gactggtggg
     6061 aggagcgacc cgggtccatc cggtggctaa gactgcagtg aagatccttg cggtgaaaag
     6121 cgcagatgac ctctcccgca aaatcgctgc ggtgggcctc tgccagaact ttgcagccct
     6181 cagggccttg gcctcagagg gaattcagag ggggcatatg tctttgcatg cccgcaatgt
     6241 ggccgtccag gcgggagcga agtgcgacct catcgagctg gttgctgccc gcatggctgc
     6301 ggagcggagg atcaatgtgg accgggcggc tgagctgata cgagagctgg aggatcgtgg
     6361 acaggaggga gtttgaagac gaggatcgga gtcctcggcc ctgaggggac ctactcggag
     6421 aaggcggctt taatctggtc agagggcatg ccctccacag tcctggtcta cttcaaggac
     6481 tttgatggag tcttgcaggg ggtggaagaa ggcgagctgg actatggaat cgtccctttg
     6541 gagaacagcc tggagggtgc ggtgacggtg gtcaatgatc ttcttctgcg cttgaatgtg
     6601 gtgatcgtgg gggaggttaa cgttcccgta aggcactgcc tggtaggcca gggggagggt
     6661 gagataaaaa tcatcctctc gcatccccag gctctggctc agtgccgcca gttcataagg
     6721 gagcactatc caggagcgga gatcaggacc acgggatcga ccagccatgc cgcccgcctg
     6781 gcccaggaat ttggggagat ggcggcaatt gccgacgcag gcgcagcaga aagatacggc
     6841 ctgcgtgtcc tggccaggga tgtccaggat gtgagggaaa acgtcacccg gttcatagta
     6901 gcagggcggc agatccccgg ggccacgggc aaagacaaaa cttcgctcat catccatctg
     6961 gctaaggaca ggccgggggc actttattcc atattgcagg agttcgccac gcgcagcata
     7021 aacctgacca gaatcgagtc caggcccagc cgcctgggtc ttggagacta ctacttctat
     7081 atcgacctgg aagggcatca ggataacagc gtggttttgg a
//
LOCUS       DAOSGP010000042         7048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_9296_length_7048_cov_14.697269, whole genome shotgun sequence.
ACCESSION   DAOSGP010000042 DAOSGP010000000
VERSION     DAOSGP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9296_length_7048_cov_14.697269"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLV49_02255"
     CDS             <1..225
                     /locus_tag="PLV49_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOG97693.1"
                     /translation="CDRARQACPICSTDIELPARTPRAREVIHRSFLDPAATRGSKEE
                     EMQAFRKVRDEMKDWISQTFGDDGRTLFSP"
     gene            complement(268..1167)
                     /locus_tag="PLV49_02260"
     CDS             complement(268..1167)
                     /locus_tag="PLV49_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOG97694.1"
                     /translation="MFYDNLATAMSFFIEIAVELIVLFIGITFLVGLIQEYVPDETIK
                     RALGGRHRILGSFLGAGFGALTPFCSCSTIPLLLGMLNAGVPFASAMAFLFASPLLNP
                     VIISLFIILLGWKITALYFSVTFLAAIVIGLMLDSLGFAAQVKSVAAVRGCCDCEQAE
                     DAKSRVQRSARFAFSLFRQLVPYLLLGAGIGAFIHGFVPTELISSIAGSGNPFAVPVA
                     AIIGVPIYIRAETMIPIGLALIEKGMSTGAVLALVIGGAGASIPELTLLSAIFKRKML
                     AAFVLTIITIAVAVGYLANWLAL"
     gene            complement(1193..1612)
                     /locus_tag="PLV49_02265"
     CDS             complement(1193..1612)
                     /locus_tag="PLV49_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG97695.1"
                     /translation="MPDSMNDEVNEESIDEVAICSLARLIGDEDRARSRTRRLLSLMK
                     AIDIDGPEGEVEVFKAVADPCRLRIVKLLKEGELCVCEIMTALDKPQSTTSHHLSILR
                     EAGLVRERKDGKWSYYRLADSAVNEMIKQARELKKIK"
     gene            complement(1699..1992)
                     /locus_tag="PLV49_02270"
     CDS             complement(1699..1992)
                     /locus_tag="PLV49_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97696.1"
                     /translation="MGLKDKIFGDKGSSGCCCGTRIVGLRQINVGGRSVGIMGLDEAL
                     HEFFKKGKKPEDSTGDELVEELKKANFIADGAEEMYKIAFLAEYQRYWEARKK"
     gene            2277..2492
                     /locus_tag="PLV49_02275"
     CDS             2277..2492
                     /locus_tag="PLV49_02275"
                     /inference="COORDINATES: protein motif:HMM:NF040962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HOG97697.1"
                     /translation="MESIVSVDERGQMVLPKEIRERAKIRPGDKLAVVSMEKDGKICC
                     LSLIRVKELEGMVKSVLGPVIDDIFKK"
     gene            2524..3351
                     /gene="arsM"
                     /locus_tag="PLV49_02280"
     CDS             2524..3351
                     /gene="arsM"
                     /locus_tag="PLV49_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOG97698.1"
                     /translation="MNDSEIKVLVKDRYGKIAGKGGSCCPTKGSCCGTLPPEEISKGI
                     GYSEEEMNAVPQGANLGLGCGNPIALASIKEGETVLDLGSGAGFDSFLAASMVGPRGK
                     VIGVDMTPEMINRARENAAIGGYSNVQFLPGELEDLPVEDNSVDIVISNCVINLVPDK
                     KKAFMEAFRVLKPGGRLMVSDLVLRQELPLRIKNSIEGYIGCISGAAMKNVYLDAIRS
                     AGFDEVEVIEESAFSLDFFVRDSTDQSTDQSPRMSAEEVNEFADSVSSIKVRAIKPL"
     gene            complement(3437..4852)
                     /locus_tag="PLV49_02285"
     CDS             complement(3437..4852)
                     /locus_tag="PLV49_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOG97699.1"
                     /translation="MKHALAIILVGLVLIFCSPLKALSESETVVAADDIMIKMRLGLP
                     VEYENAVIKGNLTSDRLGMTAKQILRIPFEVKMPPSKQVQVNSSVVIKNSIICGRVDF
                     NNTQFHKQVRFVNVFFVENALFVNSTFNDDAAFDHSIFLKEGSFEGSRFDGKANFFQT
                     RFNRPASFDGAKFGGIADFSLAQFHSVGNFASSQFSSDALFDKTDFKGEAKFIASQFN
                     QDVEFNGTRFEKETSFDGAKFCGSAQFSESVFNESVDLNSVQFEKEVRFDRTQFEGPA
                     FFAKAKFDDLVEFGDCNFLKNATFKESDFQVDAIFNNTTFLEPAEFSGSNFLGFADFG
                     GSKFLYDADFSYSIFSGPADFSRTAFRDRVNFYNTQFKKNVHFQSSQINTINLTRAGY
                     DWLYLKWDCVNHLEFDDAAYQNLINNYKRLQWLTDSSDCSSTYLKEIIRVAPDDSHKW
                     IIDGILSMVAGTNSSNSSHKG"
     gene            5182..5382
                     /locus_tag="PLV49_02290"
     CDS             5182..5382
                     /locus_tag="PLV49_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97700.1"
                     /translation="MLPRPHCCTVKLIRIARPASITDELAASHEIDLAEHEGRAIMVT
                     GVLPERKGWLCEANVIDQAGPI"
     gene            complement(5385..5573)
                     /locus_tag="PLV49_02295"
     CDS             complement(5385..5573)
                     /locus_tag="PLV49_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97701.1"
                     /translation="MCSEKVISAQEIAAFASGLDNIQNIYAEIAERYSIKLDERRDAK
                     KAIYDALKEIEAERRCCE"
     gene            5584..5823
                     /locus_tag="PLV49_02300"
     CDS             5584..5823
                     /locus_tag="PLV49_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97702.1"
                     /translation="MCSRRSCTIPGSPEVCACIEPGTYEQGWKRYMAAEIRKHVNIPI
                     IAVCNIKEPDVAEQILVEGCCDFIGVARGHLADPE"
     gene            6190..6402
                     /locus_tag="PLV49_02305"
     CDS             6190..6402
                     /locus_tag="PLV49_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97703.1"
                     /translation="MAVRIWAGYMLGHKALQIAEDVVVLERLADGEKVFKDAFAQVHI
                     IGDANRGGRILEATQEAYGRAFVFES"
     gene            6439..>7048
                     /locus_tag="PLV49_02310"
     CDS             6439..>7048
                     /locus_tag="PLV49_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOG97704.1"
                     /translation="MKNGSYFIEREKLNAFMKGAAMNEEEGLYIFWETDPKVARRVLP
                     PQLELIDPEHPQVYSYFVNIREPTFAPWYMEAGIGILGARFKEHTGLYFCNLQLSGPG
                     AQMGLLSGREMSGLPKKMCEKIVVERNDYIAHVVVEAKGRRIFEAEIEIGSYNEPMME
                     AGFTDLVPGASADGYCFLFKYDICSGKDDNVILQNATLLGYDS"
BASE COUNT         1841 a         1691 c         1779 g         1737 t
ORIGIN      
        1 tgcgaccggg ccagacaggc ctgccccatc tgcagcacag atatcgagct tcccgcccga
       61 acgcctcgtg cgagagaggt catccacaga agcttcctgg atccggcggc tacaagagga
      121 tcgaaggaag aggagatgca ggccttcaga aaagtgagag atgagatgaa ggattggatc
      181 tctcagacct ttggggatga cggcaggact cttttttcgc cctaattcgc ctgcaatatt
      241 tctatttctt ttctccttct gcaactatca caatgccaac cagttggcca gatatccgac
      301 cgcaaccgct attgtgatga tggtgagcac aaaggctgcc agcatcttcc ttttgaatat
      361 ggcggaaagg agggtcaact cagggatgct cgcgcccgct ccgccgatga ccaaggccag
      421 caccgctccc gtgctcatcc ctttctctat gagggccagg ccgatgggga tcatggtctc
      481 cgcccggatg tagatgggga cgcctatgat tgccgccacg ggaacagcaa agggatttcc
      541 cgatccggcg atgcttgata tcagctccgt gggcacaaat ccatgaatga aggcaccaat
      601 gcccgcaccc aggaggagat agggtacgag ctggcggaat aggctgaaag cgaatcttgc
      661 cgagcgctgc actctcgatt ttgcatcctc agcctgctca cagtcgcagc agcctctcac
      721 cgcggccacg gatttaacct gtgctgcgaa tcccagactg tcaagcatca ggccgattac
      781 gattgccgcc aggaaggtga cggaaaagta gagagcagtt atcttccagc ccaggaggat
      841 tatgaacagg gatattatca ccggattgag caggggcgag gcaaacagga atgccattgc
      901 agatgcgaag ggcacgcccg cattgagcat ccccaggagg agcggaatgg tcgagcagga
      961 gcagaagggg gtaagagcac caaaaccggc gcctagaaag ctgcccagaa ttctgtgccg
     1021 cccacccagg gccctcttta tggtttcatc aggcacatac tcctggatca ggcccacgag
     1081 aaaggttatc cctatgaata atactatcaa ttctactgca atctctatga aaaagctcat
     1141 agccgtcgct aggttgtcat agaacatctc atcccaccac ctaaaaatct ttttatttta
     1201 tcttcttcag ctctcgtgcc tgcttgatca tctcattgac agcgctgtct gccaggcggt
     1261 agtaggacca tttgccatcc tttcgctctc tgaccagtcc tgcctctctg agaatggata
     1321 gatgatggga ggtggtggac tgaggcttat caagagcagt catgatctcg cagacgcaca
     1381 gctctccttc cttcaagagc ttcacaatcc ttagcctgca gggatcggca acggccttga
     1441 agacctccac ctctccttcc gggccatcaa tgtcgatcgc cttcatcaat gataagagcc
     1501 ttctggtcct cgaccgcgcc cgatcctcat ctccgatgag cctggccagg ctgcatatcg
     1561 caacctcgtc gatcgattcc tcattgacct catcattcat cgaatctggc ataactgtct
     1621 atttcaccac caactactca tagatttctg gtcgagccat gagtgcagaa atggctttta
     1681 tggcctttaa attggatcct atttcttcct ggcctcccaa tacctctgat attctgccaa
     1741 aaaggcaatt ttatacatct cctctgcccc atcggcgatg aagttggcct ttttcagctc
     1801 ctcgacaagc tcatctccag tggagtcctc tggcttcttt ccctttttaa agaattcatg
     1861 gagggcttca tccaggccca tgattcctac gctgcgtcca cctacgttga tctgtctgag
     1921 tcccacaata cgggtgccac agcaacaacc gctgctaccc ttatccccga agatcttatc
     1981 tttcagtccc atgataatca cagatcatat cgatctttgc cgatatataa tgcttgcgct
     2041 ttatctgaat tctatcagga tttgatgtgc atcgcattaa aatggtttaa gaatccaaaa
     2101 tcatatttgt atagataatt acgcatcaat ttttatcgat ttgagatgtt gctttaaaac
     2161 ggagaatttt ctgatttttc agaaaatctt aaataatcag agatctcaat acgatcatgg
     2221 ctgtaaaatc cgaagacaag gcctgttgcc tgccgggatc gatgagctca tgccgagtgg
     2281 aatccatagt aagcgtagat gagcgcgggc agatggttct ccccaaagag atcagggaaa
     2341 gagcaaagat ccggccggga gataagctgg cagtggtgag catggagaag gatggcaaga
     2401 tctgctgcct atcattgatc cgggtaaaag agctggaggg catggtgaag tctgtgcttg
     2461 ggccggttat tgatgatata ttcaagaaat aatactatat ttagaatttg aggtgaacta
     2521 acgatgaacg attcggaaat aaaggtactg gtaaaggata ggtacggaaa aatagccggg
     2581 aaaggaggct cgtgctgccc caccaagggc tcatgctgtg gaactcttcc cccagaggag
     2641 atcagcaaag gtatcggcta ctcagaggaa gagatgaatg ccgtgcccca gggagcaaat
     2701 ctgggcctgg gctgcggcaa tccaatcgca ctggcatcga tcaaagaagg ggagactgtg
     2761 ctcgacctgg gctcaggggc gggattcgac agctttcttg ctgccagcat ggtcggaccg
     2821 agaggaaagg tcattggagt ggatatgacc ccggagatga tcaacagggc gagagagaat
     2881 gcagctattg gaggctatag caatgtgcag tttttgcctg gcgagctgga ggatcttccg
     2941 gttgaggata atagcgtgga tattgtcata tccaactgcg tcatcaacct ggtgcccgac
     3001 aagaagaagg cttttatgga ggcctttcgg gtgctcaaac ccggtggcag gctgatggtc
     3061 tcagatctcg tcttgcgcca ggagctgcct cttcgcataa agaattcgat cgaaggctat
     3121 ataggctgta tctcgggagc agctatgaag aacgtttatc tagatgcgat aagatctgca
     3181 ggcttcgacg aagtagaagt gattgaggaa tccgccttct cactggattt ttttgtccgt
     3241 gacagcacag atcagtcaac cgatcagagt ccaagaatga gcgcggaaga agtcaacgaa
     3301 tttgcagatt cagtatccag cataaaggta agggccatca agccgctatg atggtgggat
     3361 caggccctgg atatggggat tagaggcctg attttccttt atctattttc tgattgtata
     3421 gatccattga aaatgctcat cctttgtggg aggagttgct tgaatttgtg cctgcaacca
     3481 tcgataatat gccatctatg atccatttat ggctatcgtc tggcgcaacc ctgattatct
     3541 ctttcaagta agttgaactg caatccgatg aatctgtcag ccattgaagt ctcttatagt
     3601 tgttaatgag gttttggtaa gcagcatcat cgaactcaag gtgatttacg caatcccatt
     3661 ttaaataaag ccaatcatat ccagctctgg ttagattgat ggtattgatc tggctgctct
     3721 ggaagtggac atttttctta aactgtgtat tatagaaatt gactctatcc ctgaacgcag
     3781 ttctgctgaa gtcggccgga ccgctgaaga tggaatagct gaaatcagca tcatataaaa
     3841 acttcgaacc tccaaaatcg gcaaatccaa ggaaattgct tccgctgaat tctgctggtt
     3901 caagaaaagt tgtattattg aatatggcat caacctggaa atcggattct ttgaaggtgg
     3961 cgtttttaag gaaattacag tcgccgaact cgaccaaatc atcaaacttg gcctttgcga
     4021 agaatgccgg accctcgaat tgagtccgat caaatcgaac ctccttttca aactgaactg
     4081 aattaaggtc aacagattcg ttgaatacag attcagaaaa ttgagcagac ccgcagaatt
     4141 ttgcaccatc aaatgaggtc tctttttcga atcttgttcc attgaattcc acatcctgat
     4201 tgaactgaga tgctatgaat ttggcttctc ctttaaaatc tgttttatca aagagggcat
     4261 cagatgagaa ttggctggag gcgaaattgc caactgagtg gaactgagcg agggagaaat
     4321 cagcaatgcc gccaaattta gcgccatcaa atgatgccgg cctgttgaaa cgggtttgga
     4381 agaaattcgc cttgccgtca aaccttgatc cttcgaagct tccttccttt aaaaatatag
     4441 aatgatcaaa tgcagcatcg tcattaaaag ttgagtttac aaataatgca ttttcgacaa
     4501 aaaacacatt cacgaagcgg acctgcttat gaaattgtgt gttgttaaag tccactctgc
     4561 cgcatataat ggaatttttg atcacgacag atgaattgac ctgcacctgc ttggatggcg
     4621 gcatcttcac ctcaaagggg atgcgcaaga tctgcttggc agtcataccc agccggtcag
     4681 atgtaaggtt tccttttatg accgcatttt cgtactccac tggcaagccc agcctcattt
     4741 tgatcattat atcatcggct gctaccacag tctccgattc gctaagagcc ttcaggggag
     4801 agcaaaagat caaaaccaat ccaaccagga taatagctaa agcatgcttc acgggtcatc
     4861 tccttccaaa tattaagcct taagcttaac tttaggtcgc tttacgctct atatgcgtat
     4921 atctgagagg ctatatttct gatggataca ctccattttg ctccttagca agtctcttgc
     4981 attcgtaact gtttacccac tctgattgag gggtttgctt tccaagtata aatggttttg
     5041 cttcaatatc tatatagacg ccttcattct atagactcat tcatactgca gactccctcg
     5101 tacggccggc tctacctatg acaggagctg aggcaggaac ctctcgtgaa gctttatgga
     5161 aaccacgagg aagtttcgat cttgttgccg cgtccacact gctgcacagt caagctaatc
     5221 cgcattgcta gaccggcatc aataaccgac gagctggcag catcccatga gatcgatctg
     5281 gccgagcatg agggcagggc aatcatggtt accggtgtcc ttcctgagcg caagggatgg
     5341 ctctgtgagg ccaatgtgat agatcaggca ggaccgatat aatatcattc gcagcaacgt
     5401 cgctccgcct ctatctcttt caacgcatca taaatcgcct tcttggcatc gcggcgctca
     5461 tccaacttga tgctgtatct ctcggcgatt tcggcataaa tgttctgaat attatccagg
     5521 cccgaggcaa aagctgcgat ctcctgggca gagatcacct tttcggagca caaatgctga
     5581 ctgatgtgct ccaggaggag ctgcaccatt ccaggaagcc ctgaggtctg cgcctgcata
     5641 gagcccggca cctacgagca gggttggaag agatatatgg cagctgagat aaggaagcac
     5701 gtgaacattc caattattgc ggtctgcaac atcaaggagc ccgatgtggc cgagcagata
     5761 cttgtcgaag gatgctgcga tttcatcgga gtggcccgtg ggcatctggc agacccagaa
     5821 tgatgcaaca aggcccaagc gggaaaggcc cagacaatac gcaaatgcat tggctgcctc
     5881 tcctgcttcc aggagataga gagccaacgc catgtcaaat gcgcagtaaa tcgaaaacgg
     5941 ggaagggagc gggagttcga gaagcccaaa cacaacggcc aagggcgaac gatcgtggtc
     6001 gttggtggtg gacctgcagg catcgagggc agcgttgctc ctcgatagac gcgggtttca
     6061 tgtcgtgatc ttcgatccgg caaaacgttt aggaggtact ctgaaggccg gtcaccggac
     6121 aacaattgtc gatatgctgc acgaaatcgg ccagggcgtc cccatcattg tcctaattga
     6181 tatcaaacgg tggctgtcag aatatgggcc ggctatatgc tcggacataa ggccttgcag
     6241 atagccgagg atgtagttgt cttggagcgc ttggctgatg gcgagaaggt cttcaaggat
     6301 gcttttgcac aggtgcatat catcggggac gcaaaccgcg gtgggaggat tcttgaggcg
     6361 acacaggaag catatggtag ggcattcgtc tttgaatcct gacaacaatc ggaatggaag
     6421 aagtaaagag ggtatgttat gaagaacgga agctatttca tagaacgaga aaagctcaac
     6481 gcttttatga agggggctgc gatgaacgag gaggaggggc tctacatctt ctgggagacc
     6541 gatccaaagg tcgcgcgacg tgtactgccg ccgcaactgg aattgatcga tcccgagcat
     6601 cctcaagtct actcctactt cgttaacatc agggaaccaa ccttcgcccc atggtatatg
     6661 gaagcaggca tcggaatact tggtgctcgg ttcaaagagc ataccggcct gtatttctgc
     6721 aacctgcagc tgagcgggcc gggcgcgcag atggggctgc tctccggtcg ggagatgtcg
     6781 gggcttccca agaagatgtg cgagaagatc gttgttgagc gaaatgacta tatcgcacat
     6841 gtcgtggtag aggcaaaagg caggcgcatc tttgaggctg aaatagagat tggaagctat
     6901 aacgaaccga tgatggaggc tggattcaca gacctcgtcc ccggtgcgag cgcagacggc
     6961 tactgttttc tattcaaata cgacatatgt tcgggcaaag acgacaatgt gatcctgcaa
     7021 aacgccaccc tcctcggata cgacagcg
//
LOCUS       DAOSGP010000043         6856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_9646_length_6856_cov_17.330687, whole genome shotgun sequence.
ACCESSION   DAOSGP010000043 DAOSGP010000000
VERSION     DAOSGP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9646_length_6856_cov_17.330687"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1887
                     /locus_tag="PLV49_02315"
     CDS             256..1887
                     /locus_tag="PLV49_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG97705.1"
                     /translation="MLIAAALIGLYSILLLPLRDSEAWKKRGVTVGGISGIPLLIFLR
                     TTKGLKLLDQLSRPKWIWRTAVTIGIPLVILSMATFLILVLLMTYLMILSPPEPSSYN
                     APRNILLIPGLNEYIPFLWGWIALFVTMLVHEFAHGILSRVEGVRVKSMGIVTLLVAP
                     IAAFVEPDEEDLFGTKNKPPLVNRAARIRILSAGVIANFMVAALAMALFFGPVLGSIS
                     PVDRLIAVDVQEDSIAEEAGFESGMVLLQVNGENAIKIEELYSNLGSSMVEMEVLHNG
                     QKEIFNLPGQAARGIMVASIFPDSPADSVGLPAGSVISRIDGKEVVDVEGFRREMNLT
                     RPGQIITITTGSGKSYQVNLTSAAGQGEDEQKEESDAVGTGFIGIGISGNAIYSGGVA
                     FQEAPASEFLQILKTIPKSGVEGFAYMLNLPFSGIPGFTQKGFPGFSGWLTAVYEPVG
                     WAEPLGERFFWIANLLLWIGWINLYAGLFNCLPAGPLDGGHIFRDLVQTGFERLVPPE
                     KAEKLTRTAVAIFTWVILTSLLISIIAPFTHSISI"
     gene            2039..3148
                     /locus_tag="PLV49_02320"
     CDS             2039..3148
                     /locus_tag="PLV49_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97706.1"
                     /translation="MKICSICSIRHLAALALLMALAATAQSIEIFSLYSNIDSADITL
                     VGGGEGILQMDLTQDNKVLNSRRLDIDGPGTYVVRWPNLKVDKGSYSVCATLRDDGTK
                     DNSNDDAGEIKDGAVQSRRCYRFYYGGVEPVRFDVRDFRSDSRGLHLAISASDPTIVD
                     IYYMLVQGDRALYVTREKAVPISGLMSMAVQKDYKWKPILENGQIYEGRVKIVELNHN
                     QTRAFMNPFVAVEDAQITETYQDETGASATVMGDSRVPFEGLLRFTLSGGGEVLNITE
                     KKTPVLLSGDDETVEVTWNSTLEPGVYQLQTALIGQDGSIIDLQENIIEAKPIVVRSN
                     VDETKSSGLPAGLAGVAMLAIILFRRMRRQSQLMN"
     assembly_gap    3206..3305
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3306..3607
                     /locus_tag="PLV49_02325"
     CDS             <3306..3607
                     /locus_tag="PLV49_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97707.1"
                     /translation="KVLRAGSESTKAPKALGWKSSRRTAGIKIGLEPKSELNLSRQQE
                     SVSKFKFNSDIYTPLFIKNQYMRTKPSYIAPDNLSTREVYNVSGYPRVMLSGSIP"
     gene            complement(3668..4075)
                     /gene="trxA"
                     /locus_tag="PLV49_02330"
     CDS             complement(3668..4075)
                     /gene="trxA"
                     /locus_tag="PLV49_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG97708.1"
                     /translation="MDELEEIKKKKMEKMMSEINKPHEPTIEQPGKPIIITDATVDAA
                     SNQYPLLILDCWAEWCGPCRMIGPIIEELAAEMKGRAVFGKLNVDENPQTANRFRISA
                     IPTLMVFKDGKLIDKLVGAYPKPSLVAKIQKYL"
     gene            complement(4078..4656)
                     /locus_tag="PLV49_02335"
     CDS             complement(4078..4656)
                     /locus_tag="PLV49_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOG97709.1"
                     /translation="MYTIIACGIFKDEIERLRADLGFPFKARYLGPGLHVDFDELKDA
                     LEEELKRASSDGSQGIIVAYGQCHPKMDAILKPYHAALMDCQNCVDAFISRQAVEKKA
                     CEGLFFYLSPGWLDAWKDIFKRLGWDPEIARLHMGSFKGSVYIDTMKDAQEREEELLE
                     FFDFTNLPFTIMPMELDHFKSLIIKAIKSLED"
     gene            4767..6632
                     /locus_tag="PLV49_02340"
     CDS             4767..6632
                     /locus_tag="PLV49_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG97710.1"
                     /translation="MNLLPLGKLGARCTPEGRADFGVFLPWVSDSDGVGLGVKLIHEN
                     DQFLQDVPAKTFEMECSIDPNYGEYWHGQVEIDASEKPHKKSAWGKPGRYVYRYFLER
                     GNNEPIDWIIDPYAREFGIGKLSAFTLGYEPHIWSDEEELWKTPRQEDLIVYELMINE
                     FGGDMRTATRHLEYLQDLGVNCIEIMPVTNISNTTDWGFLPIGYFGVDERFGKRRDMQ
                     RLIDSAHQKGIAVILDMVYGHTDSLFPYSYLYREMGFGENPFLGSFAKDLFGESTDFN
                     RALTRDFFYTVNHHWLSTYHVDGFRYDCVPNYWDGPTGLGYANLVYQTYKLVFGMRGS
                     QDHWQRFFEGDEIRLIQCAEQLEEPVEVLEKTYSSSTWQNQTFDAAIAVARAEASELA
                     GRLTYLGHRLGLVGYPAKEKINEDTIQKSALQYIENHDHYRFVCNFRTISRDDNDLLK
                     EGDRRYWYKVQPYLIAIMTARGIPLIWQGQELCENYFLPESGMGRVVMFRPMRWDYFY
                     DPIGKRTIWLTRRLMKLRRKAQFLRGEHWFYDDPERYQSRGLLLFSRRAGDAFSLVAL
                     NFTDSDQKAYFSFPLGGDYYEEIHGEDSFRAGAGEWLEICIPSNYGRIWTVEGGE"
BASE COUNT         1597 a         1746 c         1896 g         1517 t
ORIGIN      
        1 tcctcggcta ccccgagcag ataagagtgg cgcgcgctgc gatagagatg ctccttgatg
       61 gtgcgcccca tggtaatgtc tacagcttcc tggagaggaa gcatgaggat ctggccaaga
      121 tggagcttga ggaacaggga ttgctctaaa gccctcttct caacatatat acatgatgcc
      181 agacatataa cgtcggattg acctgatgag gctggagttt tgagcttcga tttacagcta
      241 gaattcgcag attggatgct tatcgcagcg gcgctgatag gcctgtattc catcctcctt
      301 ttgcccttga gggatagcga ggcatggaag aagagaggtg tgactgtggg agggatatca
      361 ggaatacccc tcctcatatt cctgcggaca accaaaggat tgaagctgct cgaccagctg
      421 agccgcccca agtggatatg gagaacggcg gtaactattg gaatccctct ggtgatcctg
      481 agcatggcca cattcttgat cctcgtattg ctcatgacct acctgatgat tctctctcca
      541 cccgagccca gcagctacaa tgcacccaga aatatcctgc tcatccccgg tctgaacgag
      601 tatatcccct ttttatgggg ctggatcgcc ctattcgtca ccatgctggt gcatgagttc
      661 gcccatggca tcctatcccg agtggaaggg gtcagggtca agtccatggg catagtaacc
      721 ctgctcgttg ccccaatagc tgcttttgtt gagccggatg aggaggatct cttcggcaca
      781 aagaacaagc cgcccctggt gaacagggcc gccaggatca gaattctctc cgccggggtc
      841 atagccaact tcatggtggc cgcgctggcc atggccctct tctttgggcc agttctgggc
      901 tccatatccc ctgtggaccg gctgattgcg gtggatgtcc aggaggattc aatagcggag
      961 gaggcaggct ttgagagcgg catggtcctg ctccaggtga atggagagaa tgcgataaag
     1021 atagaggagc tttacagcaa tctgggcagc agcatggtgg agatggaggt cttgcacaac
     1081 ggccaaaagg agatcttcaa cctccccggc caggcggcaa ggggaatcat ggttgcctcc
     1141 atcttcccgg actctcccgc cgattccgtc ggcctgcctg cgggcagcgt gatctccagg
     1201 atcgacggca aggaggtggt ggatgttgag ggcttcagaa gggagatgaa cctcacccga
     1261 cccggtcaga tcatcaccat caccaccgga agtggcaaga gctaccaggt caacctcacc
     1321 tccgctgctg gccagggaga ggatgagcag aaagaggagt cggatgctgt gggaacgggc
     1381 tttatcggca ttggaatctc agggaacgcc atctattcgg gtggtgtggc attccaagaa
     1441 gctcctgcca gcgagttcct acaaatcctg aaaaccattc caaagagcgg cgtagagggt
     1501 ttcgcctaca tgctcaacct gccattttca ggaattcccg gattcaccca gaaaggattt
     1561 ccgggattca gcggttggct gactgcggtc tacgaaccgg ttggttgggc ggagcccctg
     1621 ggtgagaggt tcttctggat agcgaacctc ctcctctgga taggctggat caacctttat
     1681 gccggcctct tcaattgcct tcctgccggt cccctggatg gaggtcatat cttcagagat
     1741 ctggtgcaga cgggctttga gcggctggtt ccgcccgaga aggcggagaa gctcaccaga
     1801 acggcagtcg ccattttcac ctgggtgatt ctgaccagcc tgctaataag catcatcgcg
     1861 ccgtttaccc atagtatatc gatctgatga cttggggtct ggaagagccc gttggccgtt
     1921 gttggcagag ctctctagag ctttctgcct tctgccgcgc cggcctcatt cccctcactt
     1981 cgatacgtta gttttaaacc cattcagaaa tgacaggttg ccaggcaggt ttcgtcccat
     2041 gaaaatatgc tctatctgtt ctatccgcca tctcgcggca ttagccctac tgatggccct
     2101 ggccgcaacc gctcagtcca tagagatctt tagcctctac tccaatatag actccgccga
     2161 tatcacactg gtgggaggag gagaagggat tcttcagatg gatctgaccc aggacaacaa
     2221 ggttctgaat agcagaagac tggatatcga tggccccggc acctatgtag tgcgctggcc
     2281 aaatctcaaa gtcgataagg gaagctattc ggtctgcgca actctgcgag atgatgggac
     2341 gaaggacaac agcaacgatg atgcgggcga gatcaaggat ggtgcggtgc agtcccggag
     2401 atgctaccgg ttctattatg gcggagttga gccggtaagg tttgatgtgc gcgattttcg
     2461 ttccgattcc cggggccttc acctggcgat aagcgccagc gatccgacca ttgtggacat
     2521 ctattacatg ctggttcagg gagatcgagc actatatgta acccgggaga aggcagtgcc
     2581 catctctggt ctcatgtcca tggccgttca aaaggactac aaatggaagc ccatactgga
     2641 gaacggccag atatatgagg gccgggtcaa gattgtggag ctgaaccaca atcagaccag
     2701 ggcctttatg aacccatttg tggcggttga ggatgcccag ataactgaga cctatcagga
     2761 tgagacaggg gcaagcgcca ccgtcatggg cgactcccgg gttccttttg aggggctcct
     2821 caggttcacc ctctctggtg ggggagaggt gctcaatatc accgagaaga agacccccgt
     2881 tcttctctcc ggggatgatg agacggtgga ggtcacctgg aattccaccc ttgagccagg
     2941 ggtctatcag ctacaaaccg ccctgatcgg gcaggatggc agcataatag acctgcagga
     3001 gaacatcatc gaggccaagc caatcgtagt gaggagcaat gtggatgaga caaagagctc
     3061 aggccttcca gccggtctcg ctggagtggc aatgctggcc ataatcctct tccgaagaat
     3121 gagaagacag agccaattga tgaattagaa ggtgtgacat ggtacatgct gtcttctgcc
     3181 tggtgagcgc cctgaccatc atggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnngcaag gtcctgcgcg ctggaagcga gagcaccaag gccccaaagg ctttgggatg
     3361 gaagagcagc cgccgaacgg ctggcatcaa gattggcctt gagccaaaat ccgaactcaa
     3421 tctcagccgc cagcaggaat cggtatcgaa attcaaattc aattccgata tatacacccc
     3481 gctcttcatc aagaatcagt acatgagaac caagccctca tatatagctc cggataatct
     3541 ctccaccaga gaggtctaca acgtctccgg atatccacga gtaatgcttt ccggcagcat
     3601 tccttagggg ggcaaaggat ccgggcaatc gcattcatgc aaaaaaaaga cgaatcaaaa
     3661 gcggccttca gaggtacttt tgaatctttg ccaccagtga gggcttggga taggcgccca
     3721 caagcttatc gatgagcttt ccatccttga agaccatcag ggtgggtatc gccgagatcc
     3781 tgaacctatt ggcggtctga ggattctcat ccacattcag cttgccaaag accgcccgtc
     3841 ctttcatctc tgcagccagc tcctcgatga tcgggccgat catgcggcag gggccgcacc
     3901 actccgccca gcagtccagg atcaggagag ggtactggtt tgaagcggca tcaacggttg
     3961 catctgtgat tatgatcggc tttcctggtt gctctatggt tggttcatgg ggtttgttaa
     4021 tctcgctcat catcttctcc attttctttt tcttaatctc ttccagctcg tccaagctca
     4081 atcctccaag ctctttatag ctttgatgat cagtgatttg aagtgatcca gctccatggg
     4141 catgatggtg aagggaaggt tggtgaaatc gaagaactcc aagagctcct cttccctctc
     4201 ctgagcatct ttcatagtat cgatataaac cgaacccttg aatgagccca tatgcaggcg
     4261 tgcgatctcc ggatcccagc ccaatcgctt gaatatatcc ttccaggcat ccagccagcc
     4321 tggggagagg tagaagaaga ggccctcgca tgccttcttc tctacggcct ggcgggatat
     4381 gaaggcatcc acacagttct gacaatccat taaggcggca tggtagggtt tcaggatcgc
     4441 atccatcttg ggatggcact gtccataagc cactataatc ccctggctgc catccgatga
     4501 agccctcttc agctcctctt ccagggcatc cttaagctcg tcgaaatcaa catgcaggcc
     4561 agggcccagg tagcgagctt taaagggaaa tccgagatcg gctctcaatc tctcgatctc
     4621 atccttgaag atgccgcagg cgatgattgt gtacatggct ggagcgttct atatcatcta
     4681 taggataaat ctgtgatcct cgatactgca ttaatcattc ataatcttta tctaggataa
     4741 atggcaagtg caagtgtcag agcctcatga atctattgcc actgggaaag ttgggggcca
     4801 gatgcacgcc agagggcaga gcagactttg gcgtattctt gccctgggta tcggattcag
     4861 atggagttgg gctcggggtt aagctcatcc atgagaatga ccagttcctt caggatgttc
     4921 cggcaaagac cttcgagatg gaatgctcca ttgatccgaa ttatggagag tactggcacg
     4981 gccaggtgga gatagacgcc tctgagaagc cccataagaa atccgcctgg ggcaagccgg
     5041 gaagatatgt ataccgctat tttctggaga gagggaataa tgagcccatc gactggatca
     5101 tagaccccta tgccagggag tttggcatcg ggaagctttc cgctttcaca ttaggctacg
     5161 agcctcatat ctggagcgat gaagaagagc tatggaagac tcccaggcaa gaggatctca
     5221 tagtctatga gctgatgatc aatgagttcg gcggggacat gaggactgcc acccgccatc
     5281 tggaatacct gcaagacttg ggagtcaact gcatagagat catgccggtc accaatatat
     5341 ccaacaccac agactgggga tttctgccca tcggctactt cggggtggat gagaggtttg
     5401 gcaaaagaag agatatgcag aggctgatcg actccgccca tcaaaaaggg atagctgtga
     5461 tcctggatat ggtctacggc catacggaca gcctatttcc ctactcctac ctctaccgag
     5521 agatgggctt tggggagaat ccgtttctgg ggtccttcgc caaagacctc ttcggagaga
     5581 gcaccgactt caatcgagcc ctaacccggg acttcttcta tacggtcaat caccactggc
     5641 tgagcaccta tcacgtggat gggttcaggt acgactgcgt tcccaactac tgggacggac
     5701 ccacgggcct gggttatgcc aatctggtct atcagaccta caagctggtc tttggaatga
     5761 gaggatctca ggaccattgg cagaggttct tcgaggggga tgagatccgc ctcatccagt
     5821 gtgctgagca gctggaggag ccggtggagg tgctggagaa gacctacagc agcagcacct
     5881 ggcagaacca gacctttgat gctgccattg ccgtggcccg agccgaagcc agtgaattag
     5941 cgggccgtct gacctatctt ggccatcggc tgggccttgt cggctatcca gcgaaggaga
     6001 agatcaatga ggataccatc cagaagagcg ccctgcagta tattgagaac cacgaccact
     6061 accggttcgt ctgcaacttc cgcaccatca gcagggatga caacgatcta ctgaaggagg
     6121 gggacagaag gtattggtac aaggtccagc cctatctgat agcgatcatg actgccaggg
     6181 ggattcccct gatctggcag ggccaggagc tgtgcgagaa ctacttcctc ccggagagcg
     6241 gcatgggaag ggtggtgatg ttccggccga tgaggtggga ctacttctac gatcccattg
     6301 gaaaaaggac gatctggctg accagaaggc tgatgaagct gagacggaag gcgcagttcc
     6361 tgagggggga gcactggttc tatgatgatc ctgagaggta ccagtcccga ggactgctgc
     6421 tcttctcccg gcgggcaggt gacgctttca gcctggtggc ccttaacttc acagatagcg
     6481 atcagaaggc atacttctcc ttcccgctgg gaggcgatta ctatgaggag attcatgggg
     6541 aggacagctt cagggcaggg gcaggggagt ggctagagat ctgcatcccc agcaactacg
     6601 ggaggatctg gaccgtcgag ggcggtgaat gaagagccgc cgcctggtag accagatgga
     6661 tcacaccctg ggccgacact gctcggacga tatgaggcag gatggatcag cagcctggat
     6721 gagtcggagg gagggactcc cggacattat ttatgcaaag ctgagtatta gcccccgata
     6781 caatgaataa gccctttcca gatccctccc tctgcatgag gcatgacctt catccggggg
     6841 atatcggata tatcat
//
LOCUS       DAOSGP010000044         6820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_9714_length_6820_cov_20.138211, whole genome shotgun sequence.
ACCESSION   DAOSGP010000044 DAOSGP010000000
VERSION     DAOSGP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9714_length_6820_cov_20.138211"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..1082
                     /locus_tag="PLV49_02345"
     CDS             225..1082
                     /locus_tag="PLV49_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HOG97711.1"
                     /translation="MEIDPNLDIDLFSLRGNVVLTGNGTLPYLLLNATLRQGKASISS
                     TKYLLMRLETNRDYSFEIAKNLKLIPGDYTCTLEASGPGGILASENRRCSLAEDQEDL
                     ISKRISSGELISISDARALYAGRSLYRYESNEDEEEPEEDAGREGEKERKIERAEGTK
                     ARALISSGEEPTTKLQAPEEAEKEDSKESKAEISAEGDGAGDPELFDGSAGADDGEAS
                     VSSVGVTDESDGKEESEGNFVGSSTSKKYHLPDCRYALKIKQENKVYFQSAKEAKSQG
                     YLPCKSCHP"
     gene            complement(1093..1665)
                     /locus_tag="PLV49_02350"
     CDS             complement(1093..1665)
                     /locus_tag="PLV49_02350"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HOG97712.1"
                     /translation="MIIVGVGIGPGMMTQEAGEAIGRARLIYGSKRSIDLAREYIDPD
                     CIVREIEDYKRLSSLPEEAVVLSTGDPMLSGLGYLPGRVIPGISSLQVACARLKISEL
                     NVVPITVHGRRMDPEAIASELSRDKSVFLLTDDSTDLEGLCRLLEGRGLARSVLALTD
                     LGYPQERIDRGSTASPPRAPGLSCVLIGPL"
     gene            complement(1662..2687)
                     /locus_tag="PLV49_02355"
     CDS             complement(1662..2687)
                     /locus_tag="PLV49_02355"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HOG97713.1"
                     /translation="MPEKDPEMIADPVSGFSIPLSWIESCSDPLAREKILSGRWTILS
                     SGLLLKRGLTTGTTAAAAAKGAVVSLVRATESLEVMTPAGIPVSVSVKAENGYCNARK
                     DGGDHQSDITAGLEISARAEPSGETVLLAGVGIGRIGAGGLCDQIGRPAISPSAKGQI
                     MMAISEGLKVTGLEHVRVEIWVPQGEKISRQTLNPKLGITGGISILGSTGFVEPWNEH
                     LINERAEGLKGAKKVVVSTGRTGLKLSRILFPDHEAVLMGNQLGRLEFEPDQESVLCG
                     LPALILKWAWPEILENTGYNTVAEMVEKEPEHENIALGLRRAKEKLPRTRIVLLHRDG
                     RILLEAP"
     gene            complement(2668..3300)
                     /locus_tag="PLV49_02360"
     CDS             complement(2668..3300)
                     /locus_tag="PLV49_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HOG97714.1"
                     /translation="MIENYTDIGASTPEAIKISRKSRELISSMIGDRSLEDRITQRCV
                     IATGDPSTAEIMRFQNDPFQAGLKALERGAPIYVDIKMVQAGVLKKGHTSPIEAFIDK
                     GDELAASMGITRTSAGVLALNEKLSGSIVVIGNAPSALLTLCGFMESGRVLPDLVVGV
                     PVGFVNALESKERLREISVPSISTAGTRGGTPIAVAAINEIINTYARERP"
     gene            complement(3312..>4560)
                     /locus_tag="PLV49_02365"
     CDS             complement(3312..>4560)
                     /locus_tag="PLV49_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOG97715.1"
                     /translation="KEVYSSGMGKETDRVRAALDLLSRGPVALVSSGDANVYGMAGLG
                     LEMAEHPAEVEVVPGVTSFNAAACRAGLLFRECVAVISLSDLLTPWQDIEGRLRQAAD
                     FEMPVALYNPRSRKRDWQLLRALNIYENRDVLVAKEVGREGERLFWTTAAALMDGEAL
                     REEVDMTTLLILPGRGTYRGDVAREAEVNLVGTGPGGQANLTAEALTILSSSEKILGA
                     ERYLNLIGDYPGEKVAHAGPCPERSYARFQEAEQLARSGKRASILTGGDPSIFSAGWR
                     VMDLARNRLPVHISPGVSAFSSVAARAGAPLSGDFALLSQLDNPEAPSLLAAAGFGVV
                     IYNVKGHEVQALLQKLEPERAVALARDVARKGEEMITMKAEELMETKPSGFRFTLLVS
                     CPKAEIREGRIIARRGYDTKYSY"
     assembly_gap    4561..4660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4661..4843)
                     /locus_tag="PLV49_02370"
     CDS             complement(<4661..4843)
                     /locus_tag="PLV49_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HOG97716.1"
                     /translation="MKRLLEKYDLLVAIMAVGIVTRSLCRHLQDKWRDRPVVAVDSAL
                     SCAVPVVGGHHGANDLA"
     gene            complement(4954..5664)
                     /gene="cobM"
                     /locus_tag="PLV49_02375"
     CDS             complement(4954..5664)
                     /gene="cobM"
                     /locus_tag="PLV49_02375"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HOG97717.1"
                     /translation="MKVYFVGAGPGDPELITVKGKRLLEEADLVVYAGSLVNPQLLEG
                     LRAIKVDSNGLSIEETQAKMVEFLRAGKKVVRLHSGDPSIYGAILEQMKPLEEMGIEV
                     EVVPGVSSLFAASAALKTQLTLKGISESLIVTRPKGTTLAKDDLPTLSRLGTTMAIFL
                     GVDKIRDIVQSLDRPGTTPVAVVYHASWPDQQVLCGTIDDIADKVEEAKIDHSALILV
                     GDVVKRTGFRRSHLYRSR"
     gene            complement(5661..6269)
                     /locus_tag="PLV49_02380"
     CDS             complement(5661..6269)
                     /locus_tag="PLV49_02380"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HOG97718.1"
                     /translation="MLIGISLGPGDPQLLTFKAAAALKSCKRVYVPGEMAAELARPYS
                     SPQILDFPMIQDELELKRIWQKNADIIAQDAGASQVGFACIGDINTFSTFSHLKRVIE
                     ERHPGIEIQTIPGVGVVPALASRFDEPLERSFLVSDGSQVQSVIRIKATRPRKLAEEL
                     REEGYEDFTLGMRLFTPEERIVRGEMPERSEYFSVLLARRRE"
     gene            complement(6263..>6820)
                     /locus_tag="PLV49_02385"
     CDS             complement(6263..>6820)
                     /locus_tag="PLV49_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG97719.1"
                     /translation="DHQLTFYSSPLLLKPMKLPGTATAEENIHIAMGKLAIRPGDLFL
                     DIGCGSGAVSLAASCYTDRIYGLDRRPEAINLSRELVECGKFLLGEAEEIIPRLPAVD
                     RCFIGGTRGIDKFLPVLLEKASPGCMIVVDLARIGIASKVAELMKKEGIFREILQINI
                     NRGYDLAGDIAFKPINPIFMVIGKC"
BASE COUNT         1326 a         2044 c         1838 g         1512 t
ORIGIN      
        1 ataaggcgcg catttcaggg cttctgcttt ccactggaaa tcaaggggtt ggtcatttgg
       61 gtttgaaaaa ttttagaaag tgtggcatag cttcgatatt atttcccctt atactgttgt
      121 tatcgtccat cactggctgt atcgaattgg gatcccaacc actgcatgct tcatctcctg
      181 gcggtgtagc tcagcagtca ggggcatggt cgggtcagct gaaaatggag atagacccca
      241 atctggatat cgatctcttc agcctgcggg gaaatgtcgt tctcaccggg aatggcactc
      301 taccttatct attgctcaat gccaccctcc gccaggggaa ggcctcaatc tcaagcacga
      361 agtacctgct gatgaggctg gagacgaaca gggattacag cttcgagatc gccaagaacc
      421 tcaaactaat tcctggcgac tacacctgca ccctggaggc atcaggcccc ggcggcattc
      481 tggccagcga gaacagaagg tgcagcctgg ctgaggatca ggaggatctg atctctaaaa
      541 ggatctcctc tggagaattg atatcgatct ctgatgctcg tgccctttac gccggcagat
      601 ctctctatag atatgagtca aatgaggatg aagaagagcc agaggaggat gcagggagag
      661 agggcgagaa agagagaaaa atcgagcgtg ccgagggcac gaaagctcgt gctctgatct
      721 cgtcgggaga agagccaacc accaagcttc aggctccgga agaggcggag aaagaggatt
      781 cgaaggaatc gaaggcagag atatcggcag aaggagatgg ggcaggggat cctgagcttt
      841 ttgatggtag cgccggcgcc gatgatggtg aagcatcagt cagcagcgtt ggagtcacgg
      901 atgaatcaga tggaaaggag gagtcagaag ggaatttcgt aggcagcagc acctctaaga
      961 agtaccatct tcctgactgc cgctatgcct tgaagatcaa gcaggagaac aaggtctatt
     1021 tccagagcgc caaggaggca aaaagccagg gctaccttcc ctgcaagagc tgccatccct
     1081 gaattcatcc attcatagcg gtccgatgag aacgcaggag agaccggggg cccgaggggg
     1141 agaggcggtg ctccctctat cgatcctttc ctgggggtag ccaagatctg tgagggcgag
     1201 gacgcttcgc gcgagccccc tcccctccag cagccggcag agcccttcca gatcggtgct
     1261 gtcatcggtg aggagaaaga cggatttgtc ccggctcagc tccgatgcta tagcctctgg
     1321 gtccatcctc cgcccgtgga ctgtgatggg gaccacattc agctcgctga tcttcaggcg
     1381 ggcgcaggcc acctgcaggc tggagatccc tgggatcacc ctgcccggca gatagcccag
     1441 gccggagagc atggggtcac cggtgctcag aaccacagcc tcctctggca gagatgagag
     1501 cctcttgtag tcctctatct ccctgacaat gcagtccggg tcgatatact ccctggccag
     1561 gtctatcgac ctcttcgacc catagatcaa cctcgcccgg ccgatagcct ctccggcttc
     1621 ctgggtcatc atgcccgggc caatgcccac tcctacgatg atcatggagc ctccaataag
     1681 atccgcccat ccctatggag gagaacgata cgggtgcgag gcagcttctc tttggccctc
     1741 ctcaatccca gggcgatgtt ttcatgctcc ggctcctttt ccaccatctc cgccacggta
     1801 ttgtaccccg tattctccag tatctcgggc caagcccatt ttaatatcag agcgggaagg
     1861 ccacataaga cgctctcctg atcaggctca aactccagcc ggcccagctg gtttcccata
     1921 agcaccgctt cgtgatcggg aaagagaatt cgactgagct tgagtccggt tctacccgtg
     1981 ctgaccacca ccttcttcgc ccctttcagc ccttcagccc tctcatttat caggtgctca
     2041 ttccagggct ccacaaatcc ggtcgatccc aggatggata tgcctcccgt tatccccaat
     2101 ttggggttga gggtctggcg gctaatcttc tctccctggg gaacccagat ctccacccga
     2161 acatgctcaa gacctgtcac cttcagccct tcggatatag ccatcatgat ctgccctttt
     2221 gctgaggggc ttatggccgg gcggccgatc tggtcgcaca ggcctccggc tcctatcctt
     2281 ccaatcccca ccccggcgag gagaaccgtc tctccagagg gctctgcccg ggccgatatc
     2341 tccaggccgg cggtgatatc ggactggtgg tcccctccgt ccttgcgagc attgcagtag
     2401 ccgttctctg ccttaacgga aaccgaaacc gggattccgg ctggcgtcat cacctccagg
     2461 ctttcggtcg cccggaccag ggatacgacc gcccctttcg ctgctgctgc tgcggtggtc
     2521 cccgtggtaa gtccgcgctt taataatagg ccgcttgaga ggatggtcca tctgcccgac
     2581 aggatcttct cccgggccag gggatcggaa caggattcga tccaggagag gggtatgcta
     2641 aagccgctga ctggatcggc tatcatctca gggtctttct ctggcatagg tgtttatgat
     2701 ctcgtttatg gctgctacgg cgatgggggt tccgccgcgg gtaccggcag tggatatcga
     2761 ggggacggag atctcccgga gcctctcttt gctctccagg gcattgacaa agcccactgg
     2821 cacccccacc accagatcgg gcaggacccg tcccgactcc atgaacccgc agagggtcag
     2881 cagggcagag ggggcatttc ctataaccac tattgatccg gagagcttct cattcagggc
     2941 caatacaccg gctgaggtgc gggtgatccc catggatgcc gccagctcgt ctcccttatc
     3001 gatgaacgcc tctatggggc tggtatggcc tttctttaac acccccgcct ggaccatctt
     3061 gatatccacg tagatgggag cgccccgttc cagggcctta aggcctgcct gaaaagggtc
     3121 attctgaaat ctcatgatct cggctgtgct tgggtcgccg gtggcaatga cgcatctctg
     3181 ggtgatcctg tcctccaggc tccggtcccc gatcatgctg gatatgagct cccgactctt
     3241 tctgctgatc tttatcgcct caggtgtgct ggccccaata tcggtatagt tctctatcat
     3301 atcaatcagc tctagtagct gtatttggta tcatatcccc ttcgggctat gatcctgccc
     3361 tctcttatct ccgccttcgg gcaggatacc agaagggtga accggaagcc tgatggcttt
     3421 gtctccatca gctcttccgc cttcattgtg atcatctctt ctcccttccg ggccacgtcc
     3481 ctggccaggg ccaccgccct ctccggttcc agcttctgga ggagggcctg tacctcatgc
     3541 cctttcacat tatagatcac aacgccgaag cctgctgccg ccagcaggga tggcgcttcc
     3601 ggattatcca gctgggagag aagggcgaag tccccggaga ggggagctcc tgcccttgcg
     3661 gcaactgacg agaaggcgct cactcctgga gatatgtgaa cgggaagccg atttctggcc
     3721 aggtccatga cccgccagcc cgcggaaaag atgctcggat cgccgccggt gaggatgctc
     3781 gccctttttc ctgagcgggc gagctgttct gcctcctgga agcgggcgta tgatctctcc
     3841 gggcagggac ccgcatgagc gaccttctcc cctggatagt ccccgatcag gttcaggtaa
     3901 cgctctgctc ccagaatttt ttcagatgag ctgaggatgg tcagggcctc tgcggtaagg
     3961 tttgcctgac ctcccgggcc ggtgcccacc aggttcacct ctgcctctcg agcaacatcc
     4021 cctctgtaag tgcccctgcc cgggaggatg agaagcgtgg tcatatcgac ctcttcccgc
     4081 agggcttcgc catccatgag ggcagcggct gtggtccaaa agagcctctc tccctctctg
     4141 cccacctctt tggccaccag aacatctcta ttttcataga tattcagggc gcgcaatagc
     4201 tgccagtccc ttttccggct tctggggttg tagagggcga cgggcatttc aaaatccgct
     4261 gcctgtcgca atcggccctc gatatcctgc cagggggtga gaaggtcgct gaggcttatc
     4321 accgccacgc attcccggaa gagaaggccg gcccggcaag ctgcagcgtt gaatgatgtg
     4381 accccaggaa caacctccac ctccgccgga tgctctgcca tctccaggcc caggcctgcc
     4441 atgccataga cgttagcgtc tcccgagctc accagggcca caggcccccg gctgagcaga
     4501 tccaaagccg ctcggaccct gtctgtctcc tttcccatgc cgctggagta gacctccttt
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggccaggtca ttggccccgt
     4681 ggtggcctcc caccaccggc acagcgcagc tgagggcgga gtcgacggca accaccggcc
     4741 ggtctcgcca tttgtcctgc agatggcggc agaggcttct ggtcacaatc cccaccgcca
     4801 tgatcgctac cagcagatcg tacttctcaa ggagcctctt catcctctcc tcctcataaa
     4861 ggactatagt agattgatag cgatcgctta aggcccgggc gatcctctct cccttatccc
     4921 tatcccgggg gaagaccagg atggccaggg acttcagcgg ctccggtata ggtgagacct
     4981 cctaaatccg gttcttttaa ccacatcgcc caccagtatg agggcgctgt ggtctatctt
     5041 cgcctcttct accttatcgg cgatatcatc tattgttccg cacaatacct gctggtccgg
     5101 ccaggaggcg tgatagacca ccgccaccgg agttgtgccc ggccggtcta aggactggac
     5161 tatatccctg atcttgtcga ctcccagaaa tatggccatg gtcgttccca ggcggctgag
     5221 ggtgggcaga tcgtcctttg ccagagtggt ccctttgggc ctggttacga tcagagattc
     5281 agagatcccc ttgagggtga gctgggtctt cagggcggcg gaggcggcaa agagggagga
     5341 gactccgggg accacctcca cctctattcc catctcctcc aggggtttca tctgctctaa
     5401 tatggcgcca tagatcgaag ggtcgccgct gtgcagtcgc accaccttct ttcccgctcg
     5461 caggaactcg accatttttg cctgagtctc ctctatagac agtccattgc tgtcgacctt
     5521 gatggctctc aatccctcca ggagctgggg attgaccaga gagccggcgt acaccaccag
     5581 gtccgcctcc tctaaaagcc tcttgccctt gacggtgatc agctccgggt cgcccggtcc
     5641 cgccccaaca aagtagacct tcattctctc ctccgggcca agaggacgct gaagtactcg
     5701 ctcctctccg gcatctctcc ccggacgatc ctctcctccg gggtaaagag cctcatgccc
     5761 aatgtgaaat cctcataacc ctcttctcgg agctcctctg ccagcttcct gggcctggtc
     5821 gcctttatcc tgatcaccga ctgcacctgg gatccgtccg agaccagaaa agatctctcc
     5881 aaaggttcat cgaatctgga ggccagggct gggaccactc ccaccccggg aatggtctgg
     5941 atctcgattc ccggatggcg ctcttctatc accctcttca gatggctgaa ggtggagaag
     6001 gtgttgatgt cgccgatgca ggcaaagccc acctggctgg cgccagcgtc ctgggcgatg
     6061 atatctgcat ttttctgcca gatccttttc aactccagtt catcctggat catggggaaa
     6121 tccaggatct ggggggagga gtagggcctg gccagctctg ctgccatctc ccctggaaca
     6181 tagacccgct tgcagctctt caaggccgct gctgccttga aggtcaatag ctgcggatcg
     6241 cccggtccta ggcttatgcc gattaacatt tgccgatcac catgaatatg gggtttatgg
     6301 gcttgaaggc aatgtcccct gccaggtcgt agccccgatt gatattaatt tgcaggatct
     6361 ccctgaatat gccttccttt ttcatcagct ctgccacctt gctggctatt cctatcctgg
     6421 ccaggtcgac gacgatcatg catccggggc tggccttctc cagcaggacg ggaaggaact
     6481 tgtctatccc ccttgtgccg ccgataaagc acctgtccac tgcgggaagg cgggggatga
     6541 tctcctctgc ctcgcccagg aggaatttcc cgcattcaac cagttctctg ctgagattta
     6601 tcgcctctgg tcggcggtcc aggccgtaga tcctgtctgt gtagcatgag gcggccaggg
     6661 agactgcccc tgagccgcag cctatatcca ggaacagatc gccaggcctg atggccagct
     6721 ttcccatggc gatgtggatg ttctcctctg ctgtcgccgt gcccggaagc ttcatgggct
     6781 ttaggagaag tgggctggaa taaaaggtta attgatgatc
//
LOCUS       DAOSGP010000045         6670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_10000_length_6670_cov_18.678458, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000045 DAOSGP010000000
VERSION     DAOSGP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10000_length_6670_cov_18.678458"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            512..2788
                     /gene="nrdD"
                     /locus_tag="PLV49_02390"
     CDS             512..2788
                     /gene="nrdD"
                     /locus_tag="PLV49_02390"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOG97720.1"
                     /translation="MSSRIVQQTLEGLAVSSMPRVRTTDGHLLDWDREAIAKQLLKET
                     KLSEQFYKEPGITEEEAEDIAKEVERRVRWMNVRYLSGPLVREIMNVILLERHHTEWR
                     NICTRVGTPVFDAHLIDIGTGFESKENANLQENAETSHKKKADKISKEQYLLLLPPYL
                     ADRHLAGDLHIHDLEYFGTRPFCQDWDLRYFLYYGLMPDGLGTKASVAGPAKKPEVAI
                     LHAVKALGSAQTNFAGGQGFYNFLTFIAPYFENRNYEEIVQLMQMFVYEMTQMMVARG
                     GQLVFSSVQLTPGVPKLWRDKPAVYAGRVWDGSTLDAPLKTYGKFEREVRLAFKALME
                     VMLQGDYWGKPFNFPKPEISIEPDFMKEDEEFNAAHPDLPTYQELYLMTFKLAAEYGT
                     PYFDNQLPAYRGAGEGISCYQCCAYQFAATFSSDDQFDDKLRFRDGKHFSMGSWQVVS
                     LNCPRAAYLARGDDDLLFEYLRELMDTSVEIFKVKRQWMNQIINNHRMPFATQQPRDP
                     ITGEMGSVAVGLDALVYTIGVVGVNEMVQFHTGKQMHESKEAWKLAVRAMTEMEIYAR
                     KLSEQHGMTIALARTPAETTAQRFAVSDLIHEEFRDCAERVVKGDLDALRRNLHKTRD
                     LPVYYTNGTHLPPSADVTIFERAKLEHVFFPIVDGGNIFHVFLGEATPDPQGLMDFGL
                     RLARETQIGYFTFTKDMTVCLGCSQVSGGLEDKCPNCGSENVDHISRITGYLQAVSGW
                     NSAKRQELKDRKKYNELA"
     gene            2860..3036
                     /locus_tag="PLV49_02395"
     CDS             2860..3036
                     /locus_tag="PLV49_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG97721.1"
                     /translation="MPAVVNRDECVSCGTCVEECPEEAISLDDEEIAVVNKEKCTECK
                     TCVEACPSEAISME"
     assembly_gap    3042..3141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3333..3788
                     /locus_tag="PLV49_02400"
     CDS             3333..3788
                     /locus_tag="PLV49_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOG97722.1"
                     /translation="MAKMHSRKGGSSRSRPPMVTKAPEWSDVSKEELEKTIMKLHDTG
                     MSPSRIGLTLRDQYGVPNVKLVIGTSITGFLRDNNALADIPEDLTNLMRKALHVRKHI
                     KANKKDVHNKRALQLTENKIRRMVKYYHDSGRLAPDWTYSPETAEILIS"
     gene            3785..5107
                     /locus_tag="PLV49_02405"
     CDS             3785..5107
                     /locus_tag="PLV49_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOG97723.1"
                     /translation="MKRLDKAAEAIARSIRHCEHMRVISHNDADGITSAGLICHALMR
                     AGIPFQATLVNRLDRSVIDGLTGPVVFCDMGSGKPDLISRVQGECFVLDHHRPVGTLD
                     CMHHNPHLFGIDGAFELSAAGTVYSVVRHMGDNADLAGLALVGAMGDRQAMIGANRSI
                     LEEAVASGAVQVRAGLKLSEDGQVVDVLARSIEPLLDFTGDREKIKKFLDDLNIKGNV
                     QSLAGDDLTRLATALTLKLIMQGSFAADSILGEVIRLRHEVVENSLHMVQLLNACGNR
                     DLPGIGLTLCLRDHCALLEAERLADEYKEHILREISLLREQSRQMKNLRYLKRVNMEA
                     GAIVSGLGIRYLYTDLPLVTLNHKDDMIKISARGNKPLIARGLDLSVALRRAAEAVGG
                     NGGGHTIASGASIPPGREDEFLALLDEILDEQLNKRSDPSGQTAVDAA"
     gene            5104..5334
                     /locus_tag="PLV49_02410"
     CDS             5104..5334
                     /locus_tag="PLV49_02410"
                     /inference="COORDINATES: protein motif:HMM:NF011470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HOG97724.1"
                     /translation="MIHARLVLLAEAGEDMEMVGRSIEPDNLPNMNLLIDKRSLSLQF
                     SIEKPGTLLTTMDDLLMNIKIAKETLSVAEDR"
     gene            5331..>6670
                     /locus_tag="PLV49_02415"
     CDS             5331..>6670
                     /locus_tag="PLV49_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOG97725.1"
                     /translation="MRFHLEASLRLSADAGAAEKAISDFIRDAGPILEKGAPEGQGAK
                     ITEWRLAKNRIDLVIDSDRYVRAHDALLRLRRPLSELLGKQYRIGVRGMDVTAFEIEV
                     ESDEKISHRIPYVREMSHEGGRLRLILDVGEGGTLGQSEIENRIPDRIVSLLEEKLEK
                     GYGGKTEHWELLWESEMRVPKFTGDPTEEMQKAGWIKHGSARGQWIYGPQATHLFRTF
                     EAIVLEEIIGPLGYQEMIFPKLDTWDVWKKSGHAQGVYPEIYFVCPPKTRDPAFWEEV
                     MDYYKVTHEVPLELVKEKIDLPIGGMCYAQCPTFWGFLQGMTLPNGELPMRVFDRSGT
                     SHRYESGGIHGIERVDEFHRIEIVWLGTKEHTLEEAEKLKVCYKHIFEDILELTWRTA
                     WVTPWFMAQEGKTGLSEMTGAGTVDYEAILPYNGNWIEFQNLSVNGEKYPKGFTV"
BASE COUNT         1596 a         1723 c         1934 g         1317 t
ORIGIN      
        1 aacccgaggc ctctaccatg ccaaggtagc gatcttccag ctgatctacc tgcccttcga
       61 gccacaaggt gagcagtact ccatttaaac atgtcggtag aaagagggca ggcggccaga
      121 tgaggatcgg gccgctgagc cgcaggagct gctgtgcatc tcatcaatct cctccccagc
      181 ggggtagcag gagaaaacgt agattatgcg gccaattgtt gccgaaagat gcccttttac
      241 acctcttaag gataaaggtc atcatcttac gctggccgtc gcgatggcga aggagatctt
      301 cacctcactt tcgccctgca gggatatggt tgataataag gcatactgat agtcttaagc
      361 gtttacttta taactgagtt gatacatact tgctaaaaaa ttttgtgact gtttggagcg
      421 caaaaggaat aaactggagt ggaaatactg gtctaataga tcgatcgtcg agaggaggca
      481 taatgttgct ttattgtaga ggggatgggc gttgagcagc agaatcgttc aacagaccct
      541 ggaagggctg gctgtttcgt ctatgcccag agtcaggacc acggacggcc atctgctgga
      601 ctgggatagg gaagccatag ccaagcagct tctgaaagag accaaactat ctgaacaatt
      661 ctataaagag ccaggcataa ccgaagaaga ggcggaggac atcgccaagg aggtggagcg
      721 acgggttagg tggatgaacg tccgctacct gagcggccct ctggtcagag agatcatgaa
      781 tgtgatcctc ctggagaggc atcataccga gtggcgaaat atctgcacca gggtggggac
      841 gcccgtcttc gatgcccatc tcatcgacat cggcacaggc ttcgaatcca aggagaacgc
      901 caacctgcag gagaacgctg agaccagcca caagaagaag gcggataaaa taagcaagga
      961 gcagtacctt ttgcttctgc ccccatacct tgccgaccgc cacctggcag gagacctgca
     1021 tatccatgac ctggagtact tcggaaccag gccgttctgc caagactggg atctgcgcta
     1081 cttcctctac tacggcctga tgcccgacgg cctgggcacc aaggccagcg ttgcaggccc
     1141 ggccaaaaag ccggaggtag ccatactgca tgctgtcaag gccctgggca gcgcccagac
     1201 caactttgct ggaggacagg gcttctacaa cttcctcacc ttcatcgctc catacttcga
     1261 gaaccgcaac tatgaagaga tagtgcaact gatgcagatg ttcgtctacg agatgaccca
     1321 gatgatggtc gctcgcggcg gccagctggt cttctcctcg gtgcagctca ccccgggcgt
     1381 ccccaagctc tggcgggaca agcctgcggt ctatgcggga agggtctggg acggctctac
     1441 tctcgacgcg cctctcaaga cctacggcaa gttcgagcgc gaggtgcgcc tggccttcaa
     1501 ggccctgatg gaggtcatgc tccagggcga ctactggggc aagcccttca actttcccaa
     1561 gcccgagatc agcattgagc ccgatttcat gaaggaggac gaagagttca atgccgctca
     1621 tcccgacctt cccacctatc aggagctcta cctcatgaca ttcaagctgg cggcggagta
     1681 tggcacacca tactttgaca accagcttcc tgcctacagg ggcgcagggg agggaatatc
     1741 atgctatcaa tgctgcgcat atcagtttgc agccacattc agtagcgacg atcagttcga
     1801 cgacaagctc cgcttccggg atggtaagca cttctccatg ggctcctggc aggttgtctc
     1861 cctcaactgc cctcgtgccg cttacctcgc tcggggggat gacgatctcc tcttcgagta
     1921 tctgcgagaa ttgatggaca catcggttga gatattcaag gtgaagcggc agtggatgaa
     1981 ccagatcatc aacaaccatc gcatgccatt tgctacccag cagcccaggg atcccattac
     2041 cggcgagatg ggctccgtcg ccgtcggcct ggatgccctg gtttatacca taggcgtcgt
     2101 gggcgtcaat gagatggtgc agttccatac cggaaagcag atgcatgaat caaaagaggc
     2161 ctggaagctg gcagttcggg ccatgaccga gatggagatc tatgcccgga agctctcaga
     2221 gcagcatggc atgaccattg ccctcgcgcg cactcccgcc gagaccacgg cccagcgctt
     2281 tgccgtatcc gatctcatac acgaggagtt cagagattgt gccgagaggg tggtgaaggg
     2341 cgacctggat gcgttgagga ggaatctgca taagacgcgc gacttgcccg tctactacac
     2401 caacggtacc cacctgccac ccagcgcgga cgtcactatc ttcgagcggg caaagctcga
     2461 gcacgtattc ttccccattg ttgatggcgg caacatattc catgtcttct taggcgaggc
     2521 cactcctgat ccccagggcc tgatggactt cggcctgcgc ctggcaagag agacccagat
     2581 cggctacttc accttcacca aggacatgac cgtctgcctg ggctgctccc aggtctcggg
     2641 cggactggag gataagtgcc ccaactgcgg atcggagaat gtggatcaca tcagccggat
     2701 caccggctac ctgcaggcgg tcagcggatg gaacagcgcc aagaggcagg aactcaaaga
     2761 caggaagaaa tataatgaac tggcctagat ctgcgaaagg tatatatcgg atatacatgc
     2821 aggtggatct gcctttgagg caaattggag gactcaataa tgcccgcagt tgttaatcga
     2881 gacgaatgtg taagctgtgg aacctgcgtc gaggagtgcc cggaggaggc catcagcctg
     2941 gacgacgagg aaatagcagt cgtaaataag gaaaagtgca ctgagtgcaa gacatgtgtt
     3001 gaggcttgcc cctctgaagc aatcagcatg gagtgagtgc annnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn naagaccacc gcctggccga tgagatagaa cagctggttt
     3181 aagtactccg gaagcatgcg ctaatcttca aatccctggc ttataaacct gctaccagga
     3241 taatactgcc agaagaacgg cgcaagtttt ttatctcacc tggcagtgag aattatacct
     3301 tcgccattat gagatttaat gaggattgaa agatggcaaa aatgcatagc cgaaaagggg
     3361 gctcctctcg ttcccgaccg cccatggtga ccaaagcgcc agagtggtct gacgtgtcca
     3421 aagaggagct ggaaaagaca atcatgaagc tgcacgatac aggcatgtcc cccagccgca
     3481 ttggcctcac actgagagat cagtatggag tgccgaacgt caagctggta ataggaacga
     3541 gcataaccgg ctttctgagg gacaacaatg ctctggcgga tattccagag gacctgacaa
     3601 atctgatgag aaaagcgctc catgtgagaa agcacataaa agccaataag aaagacgttc
     3661 acaacaagag ggctctccag ctcactgaga acaagataag aagaatggtg aaatactatc
     3721 acgactccgg gcgtctggct ccggactgga cctattcgcc cgagaccgct gagattctca
     3781 tctcatgaag cggctggaca aagcggcaga agccatcgct cggtccatcc ggcattgcga
     3841 gcacatgcgg gtgatctctc ataacgatgc cgatggcatc acatccgccg ggctgatctg
     3901 ccatgccctt atgcgcgcag gcattccctt ccaggccaca ttggtcaacc ggctggaccg
     3961 gtctgtgatc gacggcctga ccgggccggt ggtcttctgc gatatgggga gcggaaagcc
     4021 ggatctgatc tcccgggtgc aaggcgagtg tttcgtttta gaccatcatc gaccggtggg
     4081 aacgctggac tgcatgcacc acaaccccca tctcttcgga atagatgggg cttttgagct
     4141 gtctgcagcg ggcaccgtct attctgtggt ccgtcatatg ggcgacaatg ccgatctggc
     4201 aggcctcgcc ctggtgggag cgatgggcga ccgccaggcc atgatcgggg cgaacagatc
     4261 catcctggaa gaggcggtgg ccagcggcgc agtgcaggtc agggccggtc tgaagctctc
     4321 agaagatgga caggtggtgg atgtcctggc aaggagcata gagccgctac tggacttcac
     4381 cggcgaccgg gagaagatca agaagttcct ggatgatctg aatataaagg gcaatgtcca
     4441 gtccctggcg ggagatgatc tgaccaggct ggctacagca ttaaccctca agctgatcat
     4501 gcagggcagc tttgctgcgg attcgatttt aggagaggtg atccgcctcc gccatgaggt
     4561 ggtggaaaac tccctgcata tggtgcagct gctcaatgcc tgcggcaata gagatcttcc
     4621 cggaattggg ctcacattat gcctgcggga tcactgcgcg ctcctcgagg ccgagaggct
     4681 ggcggatgag tacaaagaac acatcctgcg agagattagc ctccttcgag agcagagcag
     4741 acagatgaag aaccttcgct acctaaagag ggtgaatatg gaggcggggg cgatcgtctc
     4801 aggcctgggg attcggtatc tttacaccga tctgccttta gtgacattga atcacaagga
     4861 tgatatgatc aagatatctg cccgggggaa caagccactc attgctcggg gactggacct
     4921 gtctgtggcc ctgcgaaggg cagcggaggc ggtgggagga aacggtgggg gccataccat
     4981 agccagcggc gcctctatac ccccaggcag agaggatgag ttcctggccc tgttagacga
     5041 gatcctggac gagcagctga ataaaaggtc cgatccctcc ggccagacgg ctgtggacgc
     5101 ggcatgattc atgcccggct ggtcctcttg gcagaggcgg gagaggatat ggagatggtc
     5161 ggcagatcaa ttgagccgga caacctgccc aatatgaatc tgttgatcga taagagaagc
     5221 ctctccctgc agttctcaat cgagaagcct ggcactctgc tcaccaccat ggatgaccta
     5281 ttaatgaaca ttaaaatagc aaaagagacg ctatctgttg cggaggatag atgagatttc
     5341 atttagaggc aagccttcgg ctgagcgctg atgccggcgc agccgagaaa gccatttccg
     5401 attttataag ggacgctggg ccgatattgg agaagggcgc tcctgagggc cagggggcaa
     5461 agatcaccga atggcgtctg gccaaaaaca gaatcgatct ggtcatcgac agcgaccgat
     5521 atgttcgggc tcatgatgcc ctgctcaggc tgcgccgacc tctctctgag cttttaggaa
     5581 agcagtaccg gattggcgtg cggggcatgg atgtgaccgc ttttgagatc gaggtcgagt
     5641 ccgacgaaaa gatttcccat cgtatccctt atgtgcggga gatgagccat gagggaggac
     5701 ggctgcgcct tattctggat gtaggcgagg gcggcacatt aggtcagtcg gagattgaga
     5761 accgcattcc cgacaggatc gtaagccttc tggaggagaa gctggagaag ggctacggag
     5821 ggaagactga gcactgggag cttctctggg agagcgagat gagagtgcct aaattcaccg
     5881 gcgatcccac tgaggagatg caaaaggcgg gctggatcaa gcacggctct gcccggggcc
     5941 agtggatcta cgggccccag gccacacatc ttttccgcac attcgaggcc atcgtcttag
     6001 aggagatcat agggcctttg ggctaccagg agatgatatt tccaaagctt gacacctggg
     6061 atgtctggaa gaagagcggc catgcccagg gggtctaccc ggagatctac ttcgtctgcc
     6121 cgcccaagac tcgcgatcct gccttctggg aggaggtcat ggactattac aaggtcaccc
     6181 atgaggttcc cctggagctg gtcaaagaga agatcgacct gcccataggc gggatgtgct
     6241 atgcccagtg ccccaccttc tggggatttc tgcaggggat gacccttccc aatggcgagc
     6301 tgcccatgag ggtcttcgac cggtccggca ccagccaccg ctatgagtcc ggcgggattc
     6361 atggcatcga gagggtggac gagttccacc gtatagagat cgtctggctg gggacgaagg
     6421 agcataccct tgaggaggca gagaagctga aggtctgcta caagcacatc ttcgaggata
     6481 tcctggagct cacctggagg acggcctggg tcaccccctg gttcatggcc caggagggca
     6541 agaccggcct atcagagatg accggagcgg gcacagtgga ctatgaggcc atcctgccct
     6601 ataacggaaa ctggatagag tttcagaatc tgtccgtgaa cggggagaag taccccaagg
     6661 gcttcacagt
//
LOCUS       DAOSGP010000046         6632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_10087_length_6632_cov_17.791394, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000046 DAOSGP010000000
VERSION     DAOSGP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10087_length_6632_cov_17.791394"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLV49_02420"
     CDS             <1..386
                     /locus_tag="PLV49_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG97726.1"
                     /translation="SMWIRIPIFIALMGFMIYRLLGTQGVVDRIGMVFVTLCIITTSI
                     AILFGVIIAPRTWCTFCPMGTLQRIMGGSKYQLKLDGDLCIDCKRCQKVCPMQLRVFE
                     LQDLPDCIKCGRCIDACPKDALSFH"
     gene            449..1171
                     /locus_tag="PLV49_02425"
     CDS             449..1171
                     /locus_tag="PLV49_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97727.1"
                     /translation="MKHVIFLICIMAISLPLSAFAADTGYSNPNIGQSYGRGTLDEGL
                     AGMLDWLDQPVPSAKYPLPSDPSFYTAGAAESAFIPYSEYFAAATKAAESGVIGEPTQ
                     YDISGKEPIAVYYGDGQGLAYFQYASAMATRSDLWIKGTTNWSQYAVVPLGSRLELVA
                     YVPQGGDAGFYQTIQTSSIFHDYGVYQLNPGYNNMSFDADQAGRHMLYFVINNQPSNV
                     VIVDVFPPQAEAASSKPAHTIS"
     gene            1217..1888
                     /locus_tag="PLV49_02430"
     CDS             1217..1888
                     /locus_tag="PLV49_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG97728.1"
                     /translation="MSEALRILGLADLHDCIEGIRNLKNLKYDLIAFCGDLHNASSTE
                     GAKPAAMALARLGPPVLIVPGNMDHRDVVPDLWEKAGFIVLHQSSFRHKEYGFLGMGG
                     MIARDPRRLGDPNRYYHRDDEVYSVLEKAYQEVAGTRAKIVIVHQPPRGACDILYNGE
                     RSGSSELRRFIENYQPDLVLCGHIHEDRGECHIGSTLVVNVGELRRGYGAVIDLNDEI
                     TVEWI"
     gene            complement(1889..2674)
                     /locus_tag="PLV49_02435"
     CDS             complement(1889..2674)
                     /locus_tag="PLV49_02435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG97729.1"
                     /translation="MSSYSAYKGTGFDQLDPFDSSDSNLWRKAEYLGRYLFAADFLRS
                     RSIRLAADISCGLGYGSIELATASEEVVGIDGSPEMIERAMAGCQEPNIRFNCLNLEE
                     DDLCRHIREGSCGAAVSFETLEHLNDPGRAVDQFSRILMPEGFFICSVPNVISESGDD
                     AGLPKNRSHKQWFNFPSLCRMVERRGMRVIYRLGQSRSRMLFRREQQLFNARRIRRRL
                     SDETVMHSEEMIRWLSYVAAYPSVEDVDGSYSIIIVAQKQGSD"
     gene            complement(2774..3157)
                     /locus_tag="PLV49_02440"
     CDS             complement(2774..3157)
                     /locus_tag="PLV49_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HOG97730.1"
                     /translation="MKEQVEVRELKEGRYVIIDEEPCIIKSISHSKPGKHGSAKARVD
                     AIGIFDNQKKSIVQPVTTKIYVPTVERKTGQVLSIGDTSVQLMDLGSYATIDVPITED
                     QKSKIEEGKEVAYLAVMGRTKLDIR"
     gene            complement(3193..4296)
                     /locus_tag="PLV49_02445"
     CDS             complement(3193..4296)
                     /locus_tag="PLV49_02445"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG97731.1"
                     /translation="MRFACCTEEIEISGIRKCFESAMPGSVNLGLGQPDFDTPSHIKR
                     AAIRAIEMGQCGYTPNCGVPALREALAEKLVRENQIDCTADDIMVTSGASEALFLAIV
                     ALVDRGEEVLVGDPSFLSYAELTKLVGARPVSVPLDEDLRITPEAIGERITKKTRMII
                     INSPSNPTGSVQTEEQMRAIAEIADDEDIALLSDEVYEHFIYRGEHISPARFSDNVIT
                     VNAVSKTYAMTGWRLGYLAARNESIEQHLKIHQYVQACASSISQAAALEAICGPQDCV
                     RQMRDEFMSRRDLLVAGFREMGVDLTEPDGAFYLFPRVGDGDLVASKLARAGVITVPG
                     SAFGEGGREHIRISYAASRANLEEALRRMKDIL"
     gene            complement(4293..4796)
                     /locus_tag="PLV49_02450"
     CDS             complement(4293..4796)
                     /locus_tag="PLV49_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97732.1"
                     /translation="MPSSQGPRPEPVSIEISGSSVSILLTQGSINIWFGKKFDKLLLS
                     RILLMISKVDSTTEHEKEVLCRFEEISEFESNGYILTSYARKKDKYRAIFVVPFSNKR
                     ALERFMESVSTETEKEDVKITLYWRGGRVRMNIMKEELSTLGCFSILNVIYRDDQIGE
                     QNRREKP"
     assembly_gap    5006..5105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5148..6368
                     /locus_tag="PLV49_02455"
     CDS             5148..6368
                     /locus_tag="PLV49_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HOG97733.1"
                     /translation="MKLGPCIKRYKEDTHRAASPEETEERIEAKLPAAGITRVADITN
                     LDRIGIPVFSSIRPMADRGAVSVYNGKGATPVEARVSAMMEGLERYSGEVRDRELTIA
                     RYSSLKAEALNPVDLILPTEAVADADDQIPWVLGWDIMNDEEIQVPANAVFHPLSSDY
                     KRLFRTNTSGLASGNMMEEAIFHGLAEVIERDAWAIVEATRHMGPLISDVVDEQAKSL
                     LERFAAAEVDVYLRDITSDIDIPTCAAAADDIKLRDPTLLTTGMGTHTSARVAVLRAL
                     TEVAQSRLTQIHGAREDTVTADFRRQIGYERTKRLNRYWFDVGEEKSFADIQSFESDD
                     FLLDIKFMISKLEEAGLERVVVVDLTREEIGVPVVRVIVPGLEIAGVDRERVGKRIRN
                     ARSRRLPRAKPLQS"
     gene            6322..>6632
                     /locus_tag="PLV49_02460"
     CDS             6322..>6632
                     /locus_tag="PLV49_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HOG97734.1"
                     /translation="MPEVVVFLGPSLSRARAEEILQAEWRPPARRGDVYRAAQDGARI
                     IVLIDGVFFQDSSVAHKEVIYALDAGARVLGASSMGALRASELDVYGMEGVGLIYQAY
                     K"
BASE COUNT         1456 a         1773 c         1819 g         1484 t
ORIGIN      
        1 gcagcatgtg gatcaggatt cccatcttca tcgccctgat gggctttatg atctaccggc
       61 tattaggaac ccagggggtg gtggaccgga tcggaatggt atttgtcacc ctatgcataa
      121 tcaccacctc cattgccata ctctttggcg tgatcatcgc cccccggacc tggtgcacat
      181 tctgtcccat gggcacattg cagaggatta tgggaggcag caaataccag ctcaagctgg
      241 acggggattt atgcattgac tgcaagaggt gccagaaggt ctgccccatg cagcttcggg
      301 tatttgagct ccaggatttg ccggactgca taaagtgcgg ccgctgcata gatgcctgcc
      361 caaaggacgc cctgagcttt cactgattgt cgaaccgttg acgcaagttt tattaggatg
      421 tgtcgaaatc tgtttgtggt gaaatgagat gaaacatgtc atatttctta tctgcataat
      481 ggcaatcagc cttccattat cggcctttgc cgcagataca ggctactcga atcccaacat
      541 cggccagtct tatggtcgtg ggactctgga cgagggcctg gcgggaatgc tggactggct
      601 ggaccagccc gtgcccagcg ccaaataccc cctgccctcg gacccgtcct tctacacggc
      661 gggtgcagca gagagcgcat tcattcccta tagcgaatat tttgccgctg ccacaaaggc
      721 tgctgagagc ggagtgatcg gcgaacctac ccagtatgat atatctggaa aagagccgat
      781 agcagtctac tatggcgatg gtcaggggct ggcatatttc caatatgcat ctgcaatggc
      841 caccagaagc gatctatgga taaagggaac aactaactgg tcgcagtatg cagtcgtgcc
      901 cctgggaagc aggctggagc ttgtagccta tgtaccccag ggaggagatg ccggattcta
      961 ccagaccatt cagacaagca gcatattcca cgattacggc gtctatcaac tcaatcctgg
     1021 ctacaacaat atgagctttg atgccgatca agcaggaaga catatgctct acttcgtgat
     1081 caacaaccag ccgagcaacg ttgtcatagt ggacgtattc cccccgcagg ctgaagcggc
     1141 atcatccaag cccgcccata cgatatcata gagatctaaa tatctagaga tccataacat
     1201 atccaggaga tgatccatga gtgaggctct gaggattctt ggtctggcgg acctgcatga
     1261 ctgcattgag gggatccgga atctaaagaa cctcaagtat gacctgattg ccttttgcgg
     1321 cgatctccat aacgccagtt caacggaggg ggcgaagcct gctgccatgg ccctcgcccg
     1381 ccttggtccg ccagtgctga tcgttcccgg caatatggac cacagagatg tcgtccctga
     1441 tctctgggag aaagcgggtt ttattgtcct tcaccaatcc tcttttcgcc ataaggagta
     1501 cggatttctt ggcatgggag gaatgatagc ccgagatccc cgcagacttg gcgaccccaa
     1561 taggtactac catcgcgatg atgaggtcta ttccgttctg gagaaagcct accaggaggt
     1621 ggctggtacc cgggccaaga tcgtcatcgt tcatcagccg ccccgggggg cttgtgatat
     1681 tctttacaat ggcgagagat ccggctcctc tgaactgcgc cgattcatag agaactatca
     1741 gcccgatctg gtcttatgcg gccatattca tgaggatcgg ggagagtgtc atatcggctc
     1801 caccctggtg gtgaacgtag gagagctgcg tcggggctat ggagcagtga tcgatctgaa
     1861 cgatgagata acagtcgaat ggatctgatt aatctgagcc ctgcttttgg gcgacgatga
     1921 tgatggagta ggatccatcg acatcctcca cagatggata ggcggcaaca taagacagcc
     1981 agcggatcat ttcttcagag tgcatcaccg tctcgtcgct cagcctccgc ctgatcctcc
     2041 tggcattgaa gagctgctgc tctcttcgaa agagcattct tgagcgtgac tggcccagcc
     2101 gatatatcac cctcatgccg cgtcgttcca ccatccggca gagcgaggga aagttaaacc
     2161 actgcttatg ggacctattc tttggcaggc cggcgtcatc tcccgactcc gatataacat
     2221 tagggacgga acagatgaaa aagccctccg gcatgaggat tctggagaac tgatccaccg
     2281 cccggcccgg atcgttcagg tgctccaggg tctcaaagct gaccgccgcc ccgcaggagc
     2341 cttcccggat atgcctgcag agatcgtcct cctccaggtt caggcaattg aagcgaatat
     2401 tgggctcttg gcatccagcc atcgcccgct cgatcatctc cgggctgcca tctatgccca
     2461 ctacctcctc ggaggcggtg gccagctcaa tcgatccgta tcccaggccg caggatatat
     2521 cagcggcaag ccttatactc cggctgcgca gaaaatcggc ggcgaagagg tatcggccca
     2581 gatactccgc cttgcgccat agattgctgt cgctggaatc gaagggatcc agctgatcaa
     2641 aacccgtgcc cttataggcg gagtaggatg acaacttatg gcctccgagc tacccacaaa
     2701 atgtgaaatc gaaggcaggc caggaaaaga tgaacaagcc cgccttggga acaggaagcc
     2761 gatcgcttta tccctagcgg atgtccagct tggtcctgcc catcaccgct aggtaggcta
     2821 cctctttgcc ttcctcgatc ttggatttct ggtcctctgt tatgggaaca tcgatggtag
     2881 catagctgcc caggtccatc agctgcaccg atgtgtcgcc gatggacaag acctggccgg
     2941 tcttgcgctc gacagttggc acgtagatct tggtggttac cggctggacg atggatttct
     3001 tctggttgtc gaagatgcca atggcatcca ctctcgcctt ggccgaaccg tgcttgccgg
     3061 gcttggaatg ggatatgctc ttgattatgc agggctcttc atctatgatt acataccttc
     3121 cttctttcag ctctctgacc tcaacctgct ccttcatttc atcactcctg agggcgaatg
     3181 cgactcacct atttaaagga tatctttcat cctccgcagc gcttcctcga gattggcccg
     3241 gctggcggca taagagatcc ggatatgctc ccttccgccc tctccaaaag cagatcctgg
     3301 aacggtgatg acccctgctc ttgccagctt gctggccacc aggtcgccgt ctcccacccg
     3361 agggaagaga tagaaagcgc catccggttc tgtcaggtcc actcccatct cccggaagcc
     3421 tgctaccaga aggtctctgc ggctcataaa ctcatccctc atctgacgaa cgcaatcctg
     3481 gggtccacag atcgcctcca gggctgcggc ctgggagatg gagctggcgc aggcctggac
     3541 atactgatga atcttgagat gctgctctat agactcgttc cgggcggcga gataacccag
     3601 ccgccagccg gtcatggcat aggtcttgga gacggcattg acggtgatca cattatctga
     3661 aaacctagcc gggctgatat gctctccccg gtagatgaaa tgctcataga cctcatctga
     3721 gaggagggcg atatcctcgt catcggctat ttcggcgata gccctcatct gctcctcggt
     3781 ctggaccgat ccggtggggt tggaggggga gttgatgatt atcatccggg tctttttggt
     3841 tattcgctcc ccaattgcct ccggggttat gcggaggtct tcgtctaagg ggacggagac
     3901 gggccttgcc cccaccaact tagtcagctc ggcataggag aggaagcttg gatctcccac
     3961 cagcacctcc tcgccccggt ctaccagggc gacgattgcc aggaataggg cctcgctggc
     4021 accgctggtg accattatat catcggcggt gcagtcgatc tggttttctc tcaccaactt
     4081 ctctgccagt gcctctctga gcgccggcac tccgcagttg ggggtgtagc cgcactgccc
     4141 catttctatg gccctgatcg cagccctctt tatgtgagat ggagtgtcga aatccggctg
     4201 gcccagtccc agattgaccg agccgggcat agccgactca aagcatttcc taattcctga
     4261 tatctcaatc tcctctgtac aacatgcaaa cctcatggct tctccctcct gttttgctct
     4321 ccgatctgat catccctata gatcacattg agaatgctaa agcagccgag cgtgctcagc
     4381 tcctctttca tgatgttcat cctgaccctc ccccctctcc agtaaagggt gatcttcaca
     4441 tcctctttct cggtctccgt ggatacggac tccataaacc tttccagcgc cctcttattg
     4501 gagaatggga ccacgaatat cgcccggtat ttgtcctttt tgcgggcata tgatgtcaga
     4561 atatagccat tgctctcaaa ctcgctgatc tcctcaaagc ggcacaagac ctccttctca
     4621 tgctctgtgg tcgagtcgac cttggagatc attagcagaa tcctggatag aagcaactta
     4681 tcgaattttt tgccgaacca gatgttgatg gagccctgag tgagcaggat ggatacggaa
     4741 gatccactga tctctatgga gaccggttcc ggccttgggc cttgagaaga gggcattatc
     4801 tctagatcct ggcggagcag atataaccgt ttcgagaaat ggtccctgga gaggagagat
     4861 ttccatggac caatgtataa agtctctaaa ggaggagttt gcccgtgagc cctatgcccg
     4921 gatgtttggg atagagatcg aggagctgga ggcggggcac gctatcttga agatgaagac
     4981 gaacgagatg atgaacaacc tcttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnttgaa cagggtttat attccatagc tccccaagga tctggcgatg aagctagggc
     5161 cctgcataaa gagatacaag gaggacactc accgggcggc atccccggag gagactgagg
     5221 agcgaatcga ggccaagctg cccgctgccg ggatcaccag agtggccgat ataaccaatc
     5281 tggaccgaat cggtatacct gtcttctcct ccatccggcc gatggccgat cggggagcgg
     5341 tctccgtcta taatggcaaa ggggcaactc ctgtcgaggc cagggtctcg gcgatgatgg
     5401 aggggctcga acgctactct ggagaggtga gagataggga gctgacaatt gcccgctatt
     5461 ccagcctgaa ggccgaggct ctgaacccag tcgatctgat actccccaca gaagcagtcg
     5521 cggatgcaga cgaccagatc ccctgggttt tgggctggga tattatgaac gacgaagaga
     5581 tacaggttcc ggccaatgcg gtatttcatc ctctgtcctc cgactacaag aggctctttc
     5641 ggaccaacac cagcggtctc gcctcaggga atatgatgga agaagcgata ttccacggtc
     5701 tggctgaggt tatagagaga gacgcctggg caatagtgga ggcgacgagg cacatggggc
     5761 cgctgatatc tgatgttgtg gacgaacagg ccaagagcct gctggagagg tttgctgccg
     5821 cagaggtgga cgtctacctg cgggatatca ccagcgatat cgacatcccc acctgcgcgg
     5881 ctgctgccga tgatataaag ctgcgcgatc ccactctgct caccactggg atgggtaccc
     5941 acaccagcgc ccgggtggcg gtgctgcgcg ccctgactga ggtggcgcag agccggctca
     6001 cccagatcca cggggcaaga gaggataccg tcaccgccga tttccgccgg caaatcggat
     6061 acgaaaggac gaagaggctc aaccgttatt ggtttgatgt cggggaagag aagagctttg
     6121 ctgatataca atcttttgag agcgacgact tccttttgga tatcaagttc atgatcagca
     6181 agctggaaga ggcgggcctg gagcgagtag tggtggtcga tttaaccaga gaggagatcg
     6241 gcgttccggt ggtgagggtg atcgtcccgg gtcttgagat cgcaggagtg gacagggaaa
     6301 gggtgggcaa gaggataagg aatgccagaa gtcgtcgtct tcctagggcc aagcctctcc
     6361 agagctaggg cagaggagat tctgcaggct gaatggcggc ctcctgcccg gcggggtgac
     6421 gtttaccggg cggcccagga tggagcgcgg atcatcgttc tcatcgatgg ggtcttcttt
     6481 caggactctt ctgtggcgca taaagaggtc atatacgcat tggacgcagg agcgagggtc
     6541 ctgggggcgt cgagcatggg agccctgcgg gcctcggagc tggatgttta tggcatggag
     6601 ggagtagggc tcatctatca ggcctataag aa
//
LOCUS       DAOSGP010000047         6416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_10595_length_6416_cov_20.355447, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000047 DAOSGP010000000
VERSION     DAOSGP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10595_length_6416_cov_20.355447"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLV49_02465"
     CDS             <1..378
                     /locus_tag="PLV49_02465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719023.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. The beta
                     subunit is part of the catalytic core which binds with a
                     sigma factor to produce the holoenzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HOG97735.1"
                     /translation="HMVTGKMHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLIA
                     HGAALALKERLVDESDKVTELVCSHCGMIAIHDRRRNADFCPTCGADTEIYPVEMSYA
                     FKLLLDEIKSLGVAPRLILEDAV"
     gene            387..3014
                     /locus_tag="PLV49_02470"
     CDS             387..3014
                     /locus_tag="PLV49_02470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HOG97736.1"
                     /translation="MTVPKRIGKIRFGLISPQEFRKMSVVKIITADTYDDDGFPIEMG
                     LMDPRLGVIDPGLRCRSCGGRPGECPGHFGHIDLIAPVIHVGFAKLIRKVLRAICRDC
                     SRLMLKDNEKKMYLEQIQELERLGQMTDDTVNKVFAEARKNKTCPYCGAPQQEIKFER
                     PLSYIEEGHKLTASDIRDRFEKVPDEDIQVMGMNPATARPEWMILTVLPVPPVTMRPS
                     ITLESGQRSEDDLTHKLVDIIRINQRFQENREAGAPQLIIEDLWELLQYHVTTFLDNT
                     VSGVPPARHRSGRPLKTLSQRLKGKEGRFRGSLSGKRVNFSARTVISPDPNLSIGEVG
                     VPMDIAMELSVPMIANSRNMEIVKTFVRRGPDRHPGVNYVTRPDQRRVKVTDKNCEEV
                     AEQIDVGWRIDRQLADGDIVLFNRQPSLHRMSIMSHRVKVMPYKTFRLNPAVCPPYNA
                     DFDGDEMNMHVPQTEEARAEAEILMRVQENILSPRFGGPIIGGIHDYVTGSFLLTHGR
                     KPIDRRGAMELLKKFDITELPAPEGELNGEPYWTGKQIFSLILPRGLDLNFKADFCYN
                     CDVCKNEECENDAYVVIQDGQLKMGTIDAKAVGAFKGKITDRIIKEYSPSVASEFLDR
                     MTRLALRGIMHAGFSFGIDDEDIPPEAVDQIEETTRTAREKAQQLIEAYNAGELEPLP
                     GRTLDETLEMRIMQTLGKARDTAGKIAGRYLGLDNSGVVMAVSGARGSMLNLTQMAAC
                     VGQQSVRGERIMRGYAGRTLPHFRRGDLGAEAHGFVESSYKDGLNPTEFFFHAIGGRE
                     GLVDTAIRTSQSGYLQRRLVNALQDLEVKYDGTVKETRGMIVQFKYGEDGVDASRRDY
                     ASTDNVKRIIKNVLKKESA"
     gene            3011..4150
                     /gene="rpoA2"
                     /locus_tag="PLV49_02475"
     CDS             3011..4150
                     /gene="rpoA2"
                     /locus_tag="PLV49_02475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HOG97737.1"
                     /translation="MSLSEKEIQERLDGVELPQSIKESIQNELKGAEISSEEIDEIVS
                     QVKADYEHSRVEPCEAVGVVAAQSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIV
                     DARKIPSTPTMTIKLTPEYAHDRDLAREVAWAIESSSILHLGSIATDLAEMNVIIELN
                     PGALEQRKITAEEVAAKLKEETGLDVDQKENLLVMAPEEPSYRELLQLVEKIKKLSLK
                     GVEGIKRVVIRKEGDEYILYTEGSSLKKVMQFEGVDSTRIKTNNISEIGEVLGIEAAR
                     NAIINEATDTLREQGLTVDVRHIMLVADIMTVDGELKQIGRHGVSGEKASVLARAAFE
                     VTVNHILDAAVRGDVDDLKGVTENVIVGQPIQLGTGDVKLVASKR"
     gene            4156..4464
                     /locus_tag="PLV49_02480"
     CDS             4156..4464
                     /locus_tag="PLV49_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HOG97738.1"
                     /translation="MINVDRALRSSIRTGNVMLGSNRTIEAGLGGQAKLIVYALDCPA
                     DVRMQLETMDLPVYGYQGMGKDLGSACGKPFSVAALAIIEPGDSEIMALQREIRGVEA
                     "
     gene            4461..4892
                     /locus_tag="PLV49_02485"
     CDS             4461..4892
                     /locus_tag="PLV49_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HOG97739.1"
                     /translation="MSEFKLTTEGIRYIALFESLTGGMARDCVVDEENERIIFVIKKG
                     DMGAAIGRKGSNINRVKKSVGKQIEIVEYSEDVKEFLENLFQPAAIKNVMVVNKNSKR
                     LAYVEVANKDKGIAIGRDGRNILKAKMLAQRHHGVDDIIIQ"
     gene            4918..5346
                     /locus_tag="PLV49_02490"
     CDS             4918..5346
                     /locus_tag="PLV49_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOG97740.1"
                     /translation="MGNGIYAARKLESDRKTMRWSHPKFIRRMSGSKIKADPLGGAPM
                     GRGIVLEKVGIEAKQPNSAIRKAVRIQLIKNGRQVTAFAPGDGAIGFIDEHDEVLVDR
                     IGGRQGRSMGDIPGVRFKVIAVNNVSLQELVRGRKEKPVR"
     gene            5352..5909
                     /locus_tag="PLV49_02495"
     CDS             5352..5909
                     /locus_tag="PLV49_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOG97741.1"
                     /translation="MKVFGKWESIETEVLDPGVRGYMNLAPKAVMHSGGKHAKQQFKK
                     SEQHIVERLVNKMMRKEKNTGKKLKAYAIVEGAFDIIAKRTKENPLTVLGQAIANAGP
                     REEVVRLKYGGITVPKAVDTAPQRRVDTALMFIAKGAHAASFKKTKTAEAALADEIIA
                     AANRDVKSYAINHKDSVERVAKAAR"
     gene            5986..>6416
                     /locus_tag="PLV49_02500"
     CDS             5986..>6416
                     /locus_tag="PLV49_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG97742.1"
                     /translation="MTMKSWDSDYSTRGRLWGGGVRNLPDLLAGTRVLELGCGDGKTL
                     SAMPSGWRIAALDISLPALHLCSGSHPQSDLILANAERLPFKSESFEGVFAYHVAGHL
                     FLPGRRALASEVSRVLKSGGKLFFREFGWLDMRMGEGQEVE"
BASE COUNT         1626 a         1672 c         1945 g         1173 t
ORIGIN      
        1 cacatggtca caggcaagat gcacgcccgc tcccgcggcc cagtgcaggt cctcacccgc
       61 cagccaacag aaggccgggc aagggaagga ggcttgaggt ttggggaaat ggagagggat
      121 gtgctcatcg cccatggagc cgctctggcg cttaaagagc gcctggtgga cgagtcggac
      181 aaggtcacag agctcgtctg cagccattgc ggcatgatcg ccattcacga ccggcgaaga
      241 aatgctgact tctgcccgac ctgcggagca gacactgaga tctatccggt ggagatgagc
      301 tatgccttca agctcctatt ggacgagatc aaatcgctgg gagtagcacc acgactgatc
      361 cttgaagacg ctgtttaggt gtaaatatga ctgtacccaa gagaataggc aagatccgct
      421 tcggcctgat ctcgccccaa gagtttagaa agatgagcgt ggtcaagatc atcaccgctg
      481 atacttacga tgatgacggc tttcccattg agatggggct tatggaccca aggcttggcg
      541 tcatcgaccc aggcctgcgc tgccggtcct gcggcgggag gcctggagag tgccccggcc
      601 acttcggcca catcgacctc atcgccccgg tcatccacgt tggttttgcc aagctgatcc
      661 ggaaggtcct gcgggccata tgcagagact gctcccgctt gatgctcaag gacaatgaga
      721 agaagatgta cctggagcag atccaagagc tggaacggct gggccagatg accgacgata
      781 cggtgaacaa ggtattcgcc gaggccagaa agaataagac ctgcccctac tgcggcgctc
      841 cccagcagga gatcaagttc gagcggcctt tatcatatat agaggagggc cacaagctca
      901 ccgcttcaga cattcgcgac cggtttgaga aggtcccgga cgaggatatc caggtcatgg
      961 gcatgaatcc ggcgactgcg aggcctgaat ggatgatcct gaccgtcctc cctgtgccac
     1021 cggtgaccat gcgcccctcc atcaccctgg agtcgggcca gaggagcgag gacgacctca
     1081 ctcacaaact ggtggatatc atcagaataa accaacgctt ccaggagaat cgggaggctg
     1141 gagctcccca gctgatcata gaggatctgt gggagctcct gcaataccat gtaaccacat
     1201 tcctggacaa taccgtctcc ggcgttcctc ctgcccgcca ccgctccggc aggcccttga
     1261 agaccctctc ccagcgattg aaagggaagg agggacgatt cagaggatct ctatccggca
     1321 agagggtcaa cttcagcgcc agaacggtca tatcccccga tcccaacctc agcatcggcg
     1381 aggtgggcgt gcccatggat atagccatgg agctatctgt gcccatgatt gccaactcca
     1441 ggaacatgga gatagttaag acatttgtgc gccgcggtcc ggaccggcac ccgggagtca
     1501 actacgtcac ccgtcccgac cagaggaggg tcaaggtcac agacaagaac tgcgaggagg
     1561 tcgcagagca gatcgacgtc ggctggagga tcgaccgcca gcttgccgat ggcgatatag
     1621 tcctgttcaa ccgtcagcca tccctgcacc ggatgtccat catgtctcac cgggtgaagg
     1681 ttatgcccta caagaccttc cggctcaatc cggcggtctg ccctccctat aacgccgact
     1741 tcgatggcga cgagatgaac atgcatgtgc cccagaccga ggaggcccgg gcagaggctg
     1801 agatcttgat gagggtgcag gagaacatcc tatcccccag gttcggtggg cccatcatcg
     1861 gcggcatcca cgattatgtg accggctcgt ttttgctgac acacgggcga aagccgattg
     1921 accgccgggg ggcgatggag ctcttgaaga agttcgatat aaccgagctt cccgcacccg
     1981 agggagaact caatggtgag ccctactgga cgggaaagca gatattcagc ctcatcctcc
     2041 ctcgaggcct ggacctgaac ttcaaggccg atttctgcta caactgcgat gtctgcaaga
     2101 acgaggagtg cgagaacgac gcctacgtgg tcatccagga cggccagctc aagatgggga
     2161 ccattgacgc caaggcggtg ggggccttca aaggcaagat aacagacagg ataataaagg
     2221 agtacagccc ctccgtggcg agcgagttcc tggacagaat gacccgcctg gccctgaggg
     2281 gcataatgca cgctgggttt agcttcggaa tagatgacga ggacattccc cctgaagcag
     2341 tagatcagat cgaagagacc acccgtactg ccagggagaa ggcccagcag ctcatcgagg
     2401 cctataacgc aggagagctc gagcctctgc ccggccgcac cctggatgag accctggaga
     2461 tgaggatcat gcagaccttg ggcaaagctc gagacaccgc cggcaagatc gccggacggt
     2521 atctgggcct ggacaactca ggggtggtga tggcggtgag cggagccagg ggcagcatgc
     2581 taaacctgac ccaaatggcg gcctgcgtcg gccagcagtc ggtgagaggc gagcgcatca
     2641 tgcgcggcta tgctggcaga acccttcctc acttccggcg gggagacctg ggagcagaag
     2701 cacacggatt cgtggagtcc agctacaagg acggcctgaa tcccaccgag ttcttcttcc
     2761 acgccatagg cggtcgcgag ggtctggtgg atacggccat cagaacatcc cagagcggct
     2821 acctgcaaag aaggctggtc aacgccctgc aggatctgga ggtcaaatat gatggcacag
     2881 tcaaggagac gagagggatg atcgtccagt tcaagtacgg cgaggatggg gttgatgcct
     2941 ccaggaggga ctatgccagc acggataacg tcaagcgcat catcaagaac gtgctgaaga
     3001 aggagtcggc atgagcctat cagaaaagga gatccaggag agactggatg gagtagagct
     3061 tccccaaagc atcaaagagt caattcaaaa cgagttgaag ggagcagaaa taagctctga
     3121 ggagatcgat gagatcgtat cccaggtgaa ggctgactac gagcactcac gggttgagcc
     3181 ctgcgaggcg gtgggcgtgg tcgccgccca gtccataggc gagcctggca cccagatgac
     3241 gatgcgaacc ttccactacg ccggcgtggc cgagatcaac gtcaccctgg ggctccccag
     3301 gctgatcgag atcgtggacg caagaaagat cccctctacc cccacaatga ccatcaaact
     3361 gactccagag tatgcccatg atcgtgacct agccagagag gttgcctggg ccatagagtc
     3421 ctcctccata cttcacctgg gatcgattgc cactgatctc gcggagatga atgtgatcat
     3481 cgagctcaac ccaggggccc tcgagcaacg caagatcact gcagaggagg tggcagcaaa
     3541 gctgaaggaa gagacgggcc tggatgtcga ccagaaggaa aaccttctgg taatggcacc
     3601 ggaagagccc tcctaccggg agctgcttca gctggtggag aagataaaga agctctccct
     3661 caaaggtgtg gaaggaatca agagggttgt gatcaggaag gagggagacg agtatatcct
     3721 ttatactgaa ggctcgtccc tcaagaaggt tatgcagttc gagggagtgg actccacccg
     3781 aatcaagacc aacaatataa gcgagatcgg cgaggttctg ggcatagaag cggccagaaa
     3841 cgccatcatc aacgaggcca cagataccct gcgcgagcag ggtctaaccg tggatgtccg
     3901 ccatatcatg ctggtagcag acatcatgac tgtggacgga gagctcaagc agatcggtcg
     3961 acatggcgta tcgggtgaga aggccagcgt tctggccaga gcggccttcg aggtcactgt
     4021 caatcacatc ctggatgctg ccgtgagagg agatgtagac gatctcaagg gtgttacgga
     4081 aaacgtcatc gtgggccagc cgatacagct cggcaccgga gacgtgaagc tggtagcaag
     4141 caagaggtaa taacgatgat caatgtagat agagcgttaa gaagttccat cagaactgga
     4201 aatgtgatgc taggatcgaa taggaccatt gaagcaggac ttggcggaca ggcaaagctc
     4261 atagtttatg ccctcgactg ccctgcagac gtgcggatgc agctggagac catggacctg
     4321 ccggtctacg gttatcaggg aatgggcaag gacctcggat ctgcctgcgg caaacctttc
     4381 tccgtggctg cattggccat catcgagcca ggcgactcgg agataatggc actgcagcgt
     4441 gagatccggg gtgttgaggc atgagtgagt tcaagctcac cacagaagga ataagatata
     4501 tagctctgtt tgagagcctc acgggaggca tggcaaggga ctgtgtggta gacgaggaga
     4561 acgagaggat catcttcgtc atcaagaaag gagatatggg cgccgccata ggccgaaaag
     4621 gctcgaacat caaccgggtc aagaaatcgg tcggcaagca gatagaaatc gtggagtata
     4681 gcgaggacgt taaagagttc ctggaaaacc tcttccagcc cgcggccatc aagaacgtca
     4741 tggtcgtgaa caagaatagt aagcgattgg cttatgtaga ggtagcaaat aaggacaagg
     4801 gcattgccat cggcagggat ggtcgcaata tactcaaagc caagatgctt gcacagaggc
     4861 accatggcgt tgatgatatc ataattcaat gacgatgatt tattcaaggt gaaatgaatg
     4921 ggaaatggaa tctatgcagc caggaaactg gagagtgata ggaagacaat gaggtggagc
     4981 catcccaagt ttatccgcag gatgtcaggg agcaagataa aggctgaccc cctgggtgga
     5041 gcgcccatgg gaaggggtat tgtcctggag aaggtcggaa ttgaggccaa acagcccaac
     5101 tcggccataa gaaaggcagt gcgcattcag ctcattaaaa acggccgtca ggttacggct
     5161 tttgccccgg gcgatggagc catcggattt atagatgagc acgatgaggt cctggtagac
     5221 cgcatcggcg gacgtcaggg ccgatccatg ggagatatcc ctggagtcag attcaaggtc
     5281 atcgctgtca ataacgtatc cctgcaagag cttgtgcggg gcaggaaaga gaagccggtg
     5341 aggtgatcga catgaaggta ttcggcaaat gggagagtat tgagacagag gtcctggatc
     5401 ccggagtgag aggatacatg aaccttgcgc caaaagcggt catgcactcc ggcggaaagc
     5461 atgcaaagca gcagttcaag aagtctgagc agcatatcgt cgagcgtctg gtcaataaga
     5521 tgatgcgcaa ggagaagaac accggcaaga agctcaaggc ttatgccata gtggaagggg
     5581 cctttgacat catcgccaag aggaccaagg agaatcccct taccgttctg ggccaggcca
     5641 ttgccaacgc cgggccgcgg gaagaggtcg tgcgtctcaa gtacggaggc attaccgtgc
     5701 ccaaagcagt ggacaccgcc cctcagagaa gggtggacac cgccctgatg tttatcgcca
     5761 agggcgctca cgctgcctca ttcaagaaga ccaagactgc agaggcggcc ctggccgatg
     5821 agatcatcgc tgctgctaac cgcgatgtta agagctacgc cataaaccat aaggacagcg
     5881 tggagcgcgt ggccaaagca gcaagataag atcggttaca tattaaaaag gataggcgct
     5941 ggccaatcag gcacatagcg cctataaatc tgttttttta tgaacatgac catgaagtcc
     6001 tgggatagtg actactctac ccggggtcgg ctctggggag gcggagtccg caacctgccc
     6061 gatctgctcg ctggtacccg tgtcctggag ctgggctgcg gagacggcaa gaccctctcc
     6121 gctatgccta gtggctggag gatcgctgcc ctggacatat ctcttcctgc cctgcaccta
     6181 tgcagtggat cccatcccca atctgatctg atcctggcca atgctgaaag acttccattc
     6241 aaaagtgaga gcttcgaggg tgtattcgcc taccatgtgg ccggccacct cttcctgccg
     6301 gggcgcaggg ccctggccag cgaggtttcg agggtgctca aatccggtgg aaagctgttc
     6361 tttcgggagt ttggatggct cgatatgcgt atgggcgaag gccaggaggt ggaaga
//
LOCUS       DAOSGP010000048         6398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_10631_length_6398_cov_15.799937, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000048 DAOSGP010000000
VERSION     DAOSGP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10631_length_6398_cov_15.799937"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLV49_02505"
     CDS             <1..509
                     /locus_tag="PLV49_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97743.1"
                     /translation="NKLKCEYVNYRNGDRWMWMRLICLVIIFMAVPGIKGEIPLAYTP
                     AAISGGQNDVLTVKNYDTGAVMTESYTDVEHLNRDTQVRTGAYANSADVASPRGGLEA
                     SISSNVIGNAHIAWQSVDPNVTVKGRHPLIGRSVEDLTGVFSIEKLIQLWSDSQSGEI
                     CVDWLPCS"
     gene            complement(558..2534)
                     /locus_tag="PLV49_02510"
     CDS             complement(558..2534)
                     /locus_tag="PLV49_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOG97744.1"
                     /translation="MDEIKEIRSRIKGMNLTWTAGETAVSELATDEKMHYLGLVVPED
                     EKAMILQMAERDRSLAAKSGSVSIYPTQWDWRDVSGNDWTTPIKDQRQCGSCVAFATV
                     AAIESNLEIFKRDPSLDPDLSEADLFFRGCGDCCGRGWYFTPALRFAQSSGVPDEACN
                     PYQGDKTGSCPDRDKRVVKIESSKTITNSSQAKEWLSQRGPLIGGMSVYEDFFYYEKG
                     VYKTAYGGYMGDHAVCVVGFDDAKGCWICKNSWGQGWGEGGWFKIAYGECGMGSQFAF
                     YGVQFTADDDLIMPKSGRVIVRFTGKSTTLEDEIWLHRPEEKLIFRAVDSDLGKIHDL
                     GAFPAGTRLTFALKTSAGHIFYSDQLLNDDVCDHVNKTSIGTYKWQLNWEDLYGLGEK
                     DYNDVSMEIEIISATSEDLVMPKEGRVFATLKQRHAATKEEFRLNSPENRLIFTLDSP
                     IGKAIDLGKYPEGASLTFAFKTLEGYTYYTDQVKNPDFLSHVRKLPTGYNKWELRWEK
                     SYGMRLKSYKDLTAEIMVAPIVREDVILEKDSHVVARLVSKNTPNHNQFWLYKPEKKL
                     ILDSIQGNVGKSFEVGDYPAGTRLIFALQTQDGTFFYTDSGLNEDGKSHVLRLKLGSN
                     KCQLRWEDLYGLKDTDYNDLVVEIKMDPKQDPKK"
     assembly_gap    2581..2680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2975..3466
                     /locus_tag="PLV49_02515"
     CDS             2975..3466
                     /locus_tag="PLV49_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOG97745.1"
                     /translation="MEIVVKRSARRKKTIQARMVAGKMEVLAPASISDAALRAHIEKL
                     QIRLEKRIYPKDDLHLSQRAAVLNERYFQGKLSWNSIQYSSRQEHRRGSCNCAARTIL
                     ISDRLASLPQWVEDYVIVHELAHLIEPNHGKRFKALVNRYPLAERAIGFLMASETLEA
                     KGG"
     gene            complement(3495..3791)
                     /locus_tag="PLV49_02520"
     CDS             complement(3495..3791)
                     /locus_tag="PLV49_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97746.1"
                     /translation="MICKRCGRCCLHLDIFVVNPRSILPDGNLDKSDPEAMIHKPAGQ
                     ICPYLANKSKPEEKIATCTIHHLPCYRDTPCEKFEQIGPEDSLCLLSSYLDERK"
     gene            3918..4475
                     /locus_tag="PLV49_02525"
     CDS             3918..4475
                     /locus_tag="PLV49_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOG97747.1"
                     /translation="MKILVALADGFDEIEAMNVIDILRRAGIDVVTAGLKEGLVEGSH
                     GVRVMPDTILDRINHKDLDGLVLPGGAPGFINLGKDERILNMIRELNQANKYVAAICG
                     APSVLIKAGVLQGRKATVHPSGEEEVRSCAQFSSDRVVVDGNIITSRTPGTAMEFALK
                     LVEVLLGKKKMEQVKAQTLAICKGD"
     gene            complement(4554..4754)
                     /locus_tag="PLV49_02530"
     CDS             complement(4554..4754)
                     /locus_tag="PLV49_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97748.1"
                     /translation="MLDDLSHQKTALLKRRSALLKQLEQCMAEAEEIDRKISVIESCE
                     LEYDLHKNIEMITSEYRKINAI"
     gene            5537..6208
                     /locus_tag="PLV49_02535"
     CDS             5537..6208
                     /locus_tag="PLV49_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOG97749.1"
                     /translation="MVYPSKYSAINLLPELDLDKEKWSQIPGEEIIVSTAKAIEERGV
                     RVVRLPDSSQALAKIKELIPPGSEVMNGSSTTLIEIGFQELMDSGEHKWKDLKLAVTS
                     EDDAQKRARIRRKTVASDYFISGVNAIAMTGELVACDATGSRVGAWPYSAGNLLLVAG
                     VNKIVPSLDDALRRIREYTFHLEDLRSRNVYGVPSKIGKCVILANESQPGRVTLLLID
                     GSYGY"
BASE COUNT         1543 a         1547 c         1604 g         1604 t
ORIGIN      
        1 gcaacaagtt gaaatgcgaa tacgtcaatt accgaaatgg tgaccgatgg atgtggatga
       61 ggctgatttg tctggtgatc atttttatgg ccgttcccgg tatcaagggt gagatacccc
      121 tggcctatac accagccgct atcagtggcg gccagaatga tgtgctcact gtcaagaact
      181 atgataccgg tgcagtcatg accgaatcat acaccgatgt agagcacttg aatagagata
      241 ctcaggttcg aacgggggcc tacgcaaatt ccgcagacgt cgcgtcgccg agaggaggcc
      301 tggaggcgag catcagctcc aatgtcatag gcaatgctca tatcgcctgg cagtccgttg
      361 accccaacgt caccgtaaaa ggccgtcatc ctctcatcgg ccgcagcgtt gaggatctga
      421 ccggtgtatt ctccatcgag aagttgatcc agctgtggtc tgacagccag tccggcgaga
      481 tctgcgtgga ctggctgcct tgttcctgat ttctttcagc ctaatcccat gctaccagtt
      541 gaatattatc cagtcaccta ctttttggga tcctgttttg gatccatctt gatctccacc
      601 accaggtcgt tatagtcggt gtccttcagc ccgtaaaggt cctcccagcg aagctggcat
      661 ttattcgatc cgagcttcag ccgaaggacg tgcgattttc cgtcctcatt gaggccgctg
      721 tctgtgtaga agaaggtccc atcctgggtc tgcagagcga agatcagcct ggttcctgcc
      781 ggatagtctc ctacctcaaa gctcttgccc acgtttccct gaatggagtc taagatcagc
      841 ttcttctcgg gcttatagag ccagaactgg ttatgattcg gcgtattctt gctgaccagt
      901 ctggccacca cgtggctgtc tttctctaaa attacatcct cgcggacgat gggtgccacc
      961 attatctctg ctgtgagatc cttgtagctt ttgaggcgca tcccatagga tttctcccag
     1021 cgaagctccc atttgttata tccggtaggg agctttctca catggctcaa gaaatcgggg
     1081 tttttcacct gatcggtgta gtaggtgtag ccctcaagcg tcttgaaggc gaatgtgagg
     1141 ctggcgcctt cgggatactt acccaggtct attgcctttc caatggggct atccagggtg
     1201 aagatcaacc tattctccgg agagttcagc ctgaactcct ctttggtggc ggcatgcctt
     1261 tgtttaaggg tggcaaagac ccttccctct ttgggcatca ccagatcctc gctggtggcg
     1321 ctaatgatct caatctccat gctcacatcg ttatagtcct tctcccccag gccatagagg
     1381 tcctcccagt taagctgcca tttgtaggtt ccaatgctgg tcttgttaac atggtcgcag
     1441 acatcgtcgt ttaagagctg gtcgctgtag aagatatgcc ccgctgaagt cttcagggcg
     1501 aatgtgagcc gggttccggc gggaaaggct cccagatcat ggatcttgcc cagatcggag
     1561 tcaaccgccc ggaagatcag tttctcctcg ggacggtgca gccagatctc atcctccaga
     1621 gtggtgctct tgccggtaaa gcgcacaatg acccttcctg atttgggcat gatcaggtca
     1681 tcgtctgctg tgaactgaac tccatagaaa gcgaactggc ttcccattcc gcattctccg
     1741 taggcgatct tgaaccagcc gccttctccc cagccctgtc cccagctgtt cttgcagatc
     1801 cagcagcctt tggcatcatc gaagcccaca acgcatactg cgtggtcgcc catatatccg
     1861 ccgtatgcag tcttatagac tcctttctca taatagaaga aatcctcata aacgctcatc
     1921 ccgccgatga gtggaccgcg ctggctgagc cactccttgg cctgggagct gttggtaatg
     1981 gtcttcgagc tctctatctt gaccacgcgt ttgtctctgt ccggacagct gccggtctta
     2041 tccccctgat aaggattgca tgcctcgtca ggaactccgc tggactgagc aaatctcaaa
     2101 gcgggagtga aataccagcc ccgtccgcag cagtcgccgc agcccctgaa gaaaaggtcg
     2161 gcctcggaaa ggtctggatc gagggatggg tctctcttga agatctccaa attggactca
     2221 atggctgcca cagtggcaaa ggcaacacaa gagccgcact gcctctgatc ctttatcggc
     2281 gtcgtccagt cgttgccgga cacgtctctc cagtcccact gagtgggata tatggagaca
     2341 gatcccgatt ttgccgcaag agatctatcc ctttccgcca tctgcaggat catcgctttc
     2401 tcatcctccg gaaccaccag gcccaggtag tgcatcttct cgtctgtggc cagttctgat
     2461 acggctgtct cccccgcggt ccaggtcagg ttcatccctt tgatcctgct tcgaatctcc
     2521 ttgatctcat ccattcccaa gcttaataac ccccttgata tatttgaggc aaatactgag
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcatccatt cccaagctta
     2701 ataaccccct tgatatattt gaggcaaata ctgagataaa cttatgtttt ttaaaaataa
     2761 tctcatagtt aattatgttt gcggatggtg cgatagaatt ggcatagaca attgaggtga
     2821 aggcaataaa aaaaatcaga gtgaaatacc aatgatatgc atagtgaata taataaataa
     2881 gaatggccgt gaaatctggc catagtcccg gatagatttt gggaagaaag ctattaatat
     2941 ctctcaatct caatgattcc agaggctcga ttccatggag atcgtggtga agaggtctgc
     3001 gcgacggaag aagaccattc aagcccggat ggtggcggga aagatggagg tcttggctcc
     3061 tgcatccatc tcagatgctg cccttcgggc tcatatagag aagcttcaaa tcaggctgga
     3121 gaagcggatc tatcccaagg atgacctcca tttgagccag agggcagcag ttctaaatga
     3181 gagatatttc cagggaaagc tgtcttggaa cagcattcag tattcctccc gacaggagca
     3241 ccggcgcggc tcgtgcaact gtgccgcccg gaccattctc ataagcgacc gtctggcaag
     3301 ccttcctcag tgggtggagg attatgttat agtgcatgag ctcgcccacc tcatcgagcc
     3361 gaatcatggc aagagattca aggctctggt gaataggtat cctttagcgg agagggcaat
     3421 cggatttctg atggcatccg agacgctgga ggcaaaaggt ggctaaggat acatttcatt
     3481 gacccattcc aggctcattt cctttcatcc aggtagctgc tcaagaggca taaggaatcc
     3541 tctgggccga tctgctcgaa cttctcgcag ggagtgtctc tataacaggg cagatgatgg
     3601 atggtgcagg ttgcgatctt ttcctctggc ttggatttgt tggcaaggta ggggcatatc
     3661 tgcccagcag gcttgtggat catggcctca ggatcgctct tatccaggtt tccgtcggga
     3721 agaattgacc gcggattgac gacgaagatg tccagatgga ggcagcaccg tccgcagcgt
     3781 ttgcatatca tgatatgcac ccctgatggt tggatccata tttattaatt agcattgcga
     3841 ttgccaatat gagggttttc aatagcaata gacttaaggg ttcagtgcca atagcttaat
     3901 tgaggaggct ataaggcatg aagatactgg ttgctctggc agatggcttc gatgagatcg
     3961 aggccatgaa cgtcattgat atactaaggc gcgcgggaat agatgtggtg accgctgggt
     4021 tgaaggaggg attggtagag ggatctcatg gggtacgggt gatgcctgat accatcctgg
     4081 acaggatcaa ccataaagat ctggacggcc tggtccttcc tgggggagcg cccggcttca
     4141 tcaacctggg aaaggatgaa cggatattga atatgatcag ggagctgaat caggcgaaca
     4201 agtatgtggc agctatatgc ggagcgccat cggtcctgat taaagcggga gtcttgcagg
     4261 gccggaaggc cactgtgcat ccttctgggg aggaggaggt aaggtcgtgc gcccagttct
     4321 ccagtgatcg ggtggtggtg gatggaaata tcataaccag ccgcactccg ggaacggcga
     4381 tggagttcgc cttgaagctg gtggaggtat tattgggcaa gaagaagatg gagcaggtca
     4441 aagctcagac cctggcgata tgcaaaggag attaaaattt cgcagtcctt gccgattcat
     4501 ttacttcatt gagccatccc cctcaatgcc acatctcaat atctcagcat tgcctaaatg
     4561 gcatttattt tcctgtattc tgatgttatc atctctatgt ttttatgcag gtcgtattcg
     4621 agttcacagc tctctatcac tgagatcttt cgatctatct cctctgcctc cgccatgcat
     4681 tgctctaatt gctttaacag tgccgacctg cgtttgagca aagcagtttt ttggtgtgag
     4741 agatcatcta acacagacac tttcatcttc ctccatttca atgcatctca cctaatatct
     4801 ataactatta attattaagt ataagcataa aaggatttcg gtctcagaaa cagagctttg
     4861 tcacagcaat tacagataat ctctcatcca aaacagactt tactctttag ccggcgccga
     4921 ttaatacccc gagaggcggt tttcatgctt gctcggactc ataaaggggt caccgatcag
     4981 catgattggt cagcagtctg gatcagcatg gctaataagt acgaataata agaatcaatg
     5041 atcagcatgg tcgctcagtg cgcaatcagc gctggcagag aagactcatc catagtttag
     5101 ctgcagtatc tgaatgtcat gccaatctcg aaaagaaatt catgaactgc gggggaatgc
     5161 tttatccatt gcaagactac aattccctct atgctgcatc taaatcggaa aaattttagg
     5221 ctattcgcta tgtggtgtgg gtaaggaatg ctttgtttct ctatgcgcaa ctctgcgaac
     5281 tctgcgactc tgcggttttg cttcgcaaac attagttata accagtgtct tgagaccatt
     5341 taaccgcaga ggcacagagc gcacagagga ctacggaaaa attgttggat tcctgcgcca
     5401 ggcgcatgaa tccacagaaa acagcgatga gccaaatttt gtatgccctc aaggcttttg
     5461 cccttggggc aatctgccta ttgactttgt gcaaatataa tggtgaggcg atcaattatt
     5521 ctgggaggag atagagatgg tgtacccaag caaatatagc gcaattaacc tactacctga
     5581 gcttgatctt gataaagaga aatggagcca aatacctggt gaagagatca ttgtatcaac
     5641 cgcgaaagcc atcgaagaga gaggtgtccg cgtggtgcga ttaccagata gcagccaggc
     5701 tttagccaag atcaaagagt tgatccctcc tggttcggag gttatgaatg gctcatcgac
     5761 caccctcatc gagataggct ttcaggagct tatggatagc ggcgagcaca aatggaagga
     5821 tctgaagctg gcggtgactt cagaggacga tgctcagaaa agagccagga tcaggcgaaa
     5881 gactgtagct tccgattact tcatttccgg agtgaatgcc atagccatga ccggggagct
     5941 ggtggcctgc gatgctacgg gaagcagagt aggcgcgtgg ccctacagcg cagggaacct
     6001 gctgctggta gcaggagtaa ataagatcgt cccctcatta gatgatgctc tgcgccgcat
     6061 aagagaatac accttccacc ttgaggatct tcgctcccgg aatgtatatg gcgttccgag
     6121 caagattgga aaatgcgtca tcctcgccaa tgagtcgcag cctggacggg tcacgcttct
     6181 attgatagat ggcagctatg gatattgaat agtgataata aaaaattgtg gggtaattgt
     6241 cctggccaag caatcatgcc cggccagccg atcgagcccc tcgattgcta accaatcgtt
     6301 caatttttaa cgcagctgga aagccagata gatggtggcc atacctccca caattgccag
     6361 cactgctaag agatagatca aaatggcgat accaacaa
//
LOCUS       DAOSGP010000049         6383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_10679_length_6383_cov_17.781922, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000049 DAOSGP010000000
VERSION     DAOSGP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10679_length_6383_cov_17.781922"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="tuf"
                     /locus_tag="PLV49_02540"
     CDS             complement(<1..955)
                     /gene="tuf"
                     /locus_tag="PLV49_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HOG97750.1"
                     /translation="MADTKPHLNLAFIGHVDHGKSTTVGRLMFETGAVDPHVIDEYKK
                     EAAGKGKATFEFAWVMDSLKEERERGVTIDIAHHRFDTAKYYFTVVDCPGHRDFVKNM
                     ITGASQADAAVLIVAVPDGVMAQTKEHVFLSRTLGVNQLVVAMNKIDATTPPFDEKRY
                     NEVKEEVGKLLKMVGYKVDDIPFIPLSGLMGDNLAKASTNTPWYKGPTLLEALNNLKV
                     PDKPTNLPLRVPVQDVYTISGVGTVPVGRVETGVMRKNDKIVFQPANVTGEVKSIEMH
                     HEEVPEAFPGDNIGWNVRGVSKKDIRRGDVCGSVEKPPTVAK"
     gene            complement(1033..3228)
                     /locus_tag="PLV49_02545"
     CDS             complement(1033..3228)
                     /locus_tag="PLV49_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HOG97751.1"
                     /translation="MGKRKKIAERVMTLMDKPQFIRNIGIVAHIDHGKTTLSDNLLGG
                     AGMISMDLAGKQLFMDFDPLEQARGITIDAANVSMVHEVDGKEYLINMIDTPGHVDFG
                     GDVTRAMRAVDGAVVVVDAVEGAMPQTETVLRQALKEGVRPVLFVNKVDRMINELKVE
                     KKDMAARLGKVIDNVNKLILGMDEEKYKAGWKLDAAKGNIAFGSALYNWAISVPQMKK
                     TGIGFDQVVDYCHEGKMKELAEKVPLYIAVNDMVVKFLPSPIEAQKERVGVIWHGDWD
                     SPVGKAMASCDPNGPVAFMINKVKVDPHAGEVATGRLFSGTLRRGMELYVSGFGGTNR
                     IQQTGVVMGAERVEVEEIPAGNIAAVTGLRDAIVGSTISSEENMTPFEQIKHVSEPVM
                     TVAVEAKNTRDLPKVVEVLRQIAKEDPTLQVTINEETGEHLMAGMGELHLDVTVTRLQ
                     RDRGVELKTSPPIVVYRESIAGHAGPVEGKSPNHHNRFYIEFEPLEEGVIQSLKEGKI
                     SMKMEEIERRKLLIENGMDKDEARNISGYYGTNVLLNMTKGIQYLRETMELILEGFEE
                     ALKNGPICREPVQGIKAKIMDVKLHEDAVHRGPAQVIPAVRQAVQAGILMADPVLLEP
                     FQNVYIQVPQDQMGGAMSEIQGRRGVILNMDSQGDMVILKSKMPVAQMFGFSGAIRSA
                     TEGRALWSTEFAGFEPLPANLLLEAVKQIRTRKGLKPEMPKPSDYLKVV"
     assembly_gap    3321..3420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3421..4838
                     /locus_tag="PLV49_02550"
     CDS             <3421..4838
                     /locus_tag="PLV49_02550"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOG97752.1"
                     /translation="SSGSVFEMARLVMKFGGVSVADGNRLRHVGDLVRHFHRDNEIVL
                     VTSALQGVTDELLACARRAASEGNVSDVMDFMERITDRHNQAIADAIQNPAIAKEVQG
                     IISEKLSLLEKAYIGICYLGELTRRSLDYISGYGEQLAAPILSGVLRDMGIESQHYTG
                     REAGIITDSNYGDARPLEKTYGLIPQKLLPLKGVPVVTGFIAQDEKGTNTTLGRGGSD
                     LSASLIGAAINADEIWFWKETSGIMTTDPKIVPEAKTIPTISYREAMELSYFGAKVLH
                     PRAIEPAIKKGIPVRVKCTFDPEDPGTQIVRDDVPKEDVIKAVTLHQKVELLNISGAG
                     MIGTLGVAARAFTALAEAGINIVMISQGSSEANISMVIEEGQADRADEALRSEFPRDV
                     VKEISHDHDVCTVAVTGSGMAGTPGVAARVFKAMGQAKINVVMISQASGQHNISFVVA
                     SADGERAVRELHREFGLGGEA"
     gene            4835..5830
                     /gene="purM"
                     /locus_tag="PLV49_02555"
     CDS             4835..5830
                     /gene="purM"
                     /locus_tag="PLV49_02555"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOG97753.1"
                     /translation="MKKLTYAQAGVDISLENRSIEAMKSVLTTRRKGFGAPLTDIGHY
                     AGLLDMGSFALAMTTDGVGSKVLIANAISKWDTVGIDCIAMNVNDLYAIGAEPIAFVD
                     YLAVEKVDPERAEQIAIGLARGAELSNMTIIGGETASLPEIIRGFDLAGTAIGVVDKD
                     KVVTGEKIKKGDVLVGVPSSGLHSNGYTLARAIVAQSGYTYFDRLPGGEKSIGEELLV
                     PTRIYHEIVELVGRCDVHGLAHITGSGLLKLRRISNLGFEITDPLEPQPIFHFLQEQG
                     RVEDLEMYQTFNMGMGFLVVLEEEEAQEACRIMGPGSRVIGSVVGEGLRAGHLAF"
     gene            complement(5858..>6383)
                     /locus_tag="PLV49_02560"
     CDS             complement(5858..>6383)
                     /locus_tag="PLV49_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobN"
                     /protein_id="HOG97754.1"
                     /translation="MCGCTGPDNVKLVVMGTIDSGGSGCMCPANAFVKALLRHVITRE
                     KDLVILDMEAGIEHLGRATARGVDAMIAVVEPGMRSIETVERIMQLGKEIGISRYFAV
                     INKADNPGPVADKLSAMGIPVLGTIPFDRCLVEADLAGTPPIDADCPSIQSIKRIKEK
                     LEEMFGENKIEKNE"
BASE COUNT         1290 a         1803 c         1705 g         1485 t
ORIGIN      
        1 ccttggcaac ggttgggggc ttctccaccg atccgcagac atctccccgg cgaatatcct
       61 tcttggagac gccgcgcaca ttccatccga tgttatctcc ggggaatgcc tcaggaacct
      121 cctcatgatg catctcaatg gacttgacct cgccggtcac atttgcgggc tggaagacta
      181 tcttatcgtt ctttctcatg acaccggtct ctactctgcc cacgggcaca gtgccgacac
      241 ctgagatggt atagacgtcc tgcaccggta cgcgcagagg caagttggtg ggcttatccg
      301 ggaccttgag gttgtttaag gcctcgagca gggttgggcc cttgtaccag ggggtattgg
      361 tgctggcttt ggccaggtta tctcccatca gaccggatag gggtatgaat ggtatatcat
      421 caaccttgta tccaaccatc ttgaggagct tgcctacctc ttccttgacc tcgttgtacc
      481 tcttctcgtc aaatggcgga gtggtagcat cgatcttgtt catagccaca accagctggt
      541 tgactcccag ggtcctggac aggaatacgt gctccttggt ctgagccatg actccgtccg
      601 gtacggccac gatcaatacg gcagcatctg cctggctggc cccggtgatc atgttcttca
      661 cgaagtccct gtgaccaggg caatccacca ctgtgaagta gtacttggcg gtatcgaacc
      721 gatgatgggc gatgtcgatg gtaacgcccc tctcgcgctc ttccttcagg ctgtccatga
      781 cccaggcgaa ctcgaaggtt gccttacctt tgcctgctgc ttctttctta tactcatcaa
      841 tgacgtgggg gtctacagcc ccggtctcga acatcaatct gcctacggtt gtcgactttc
      901 cgtggtcgac gtgtccaatg aatgctagat tgagatgcgg cttagtatct gccataactt
      961 atctcctcca actggcacta tccatatttt tcagtatttc tgcttttatg tttaatcttt
     1021 tcgaaaggcc acttatacga ccttcaagta atcactgggt ttgggcatct ccggcttcaa
     1081 gccctttctg gtcctgatct gcttgactgc ctccagtagc aggtttgcgg gcaggggctc
     1141 gaatcctgcg aactccgtcg accagagggc tctcccttcg gtggccgatc ttatcgcccc
     1201 ggagaaaccg aacatctgag ccacaggcat cttggattta aggatcacca tatcgccctg
     1261 gctgtccatg tttaggatga cgcctcttct cccctggatc tcagacatgg ctccacccat
     1321 ttgatcctga ggcacctgta tatagacgtt ctggaagggc tcgagcagca cgggatctgc
     1381 cattaggatc cctgcctgca ctgcctgcct gactgcggga atgacctgag cggggccgcg
     1441 gtgcactgca tcctcgtgca gcttcacatc catgatcttg gcctttatcc cctggaccgg
     1501 ttccctgcag atggggccgt tcttcaaggc ctcctcgaag ccttccagta tgagctccat
     1561 ggtctctctc aggtactgaa tgcctttggt catattaaga aggacattgg tcccgtagta
     1621 gccggatatg ttccgggcct catccttgtc catgccattt tcgatgagca gctttctgcg
     1681 ctcaatctcc tccatcttca tgctgatctt gccctctttc agggactgga tcactccttc
     1741 ttccagaggc tcaaactcga tatagaagcg gttatgatgg ttgggcgact ttccttctac
     1801 tggccccgca tggccggcta tggactcccg gtataccaca atgggcggag aggtcttcag
     1861 ctctacaccg cgatcgcgct ggagcctggt gacggttaca tccaaatgca gctctcccat
     1921 gcctgccatg agatgctctc cggtctcctc attgatggtg acctgcaaag tcgggtcctc
     1981 cttggcgatc tgcctcaata cctcgaccac cttgggcagg tcgcgggtgt tcttggcctc
     2041 gaccgccacg gtcatgaccg gttcggagac atgcttgatc tgctcgaagg gggtcatatt
     2101 ttcctctgag gatatagtgg atccgacgat cgcatcccta aggccggtaa ccgcggcgat
     2161 gttccctgcc gggatctcct ctacctctac cctctccgca cccattacca cgccggtctg
     2221 ctgaatgcgg ttggttccac cgaagccaga tacataaagc tccatgcccc gcctgagtgt
     2281 cccggagaag agccttcctg tggccacctc gccagcatga gggtcgacct tgaccttgtt
     2341 gatcatgaaa gccaccggcc cattcggatc gcatgaagcc atggccttcc ccaccgggct
     2401 gtcccaatcg ccgtgccaga tgaccccgac ccgctccttt tgggcctcga tgggactggg
     2461 caggaacttg accaccatat cgttgactgc aatgtacagg ggaaccttct ctgccagctc
     2521 cttcatcttt ccttcatggc agtagtctac cacctgatca aagccgatcc ccgtcttctt
     2581 catctgggga acgcttattg cccaattata gagagccgat ccgaaggcga tatttccctt
     2641 ggcggcatcc agcttccagc cggccttata tttctcctca tccattccta aaatgagctt
     2701 gttgacattg tctatgacct tgccaaggcg ggcggccatg tccttcttct ccaccttgag
     2761 ctcgttgatc atccggtcca ctttattgac gaaaaggacc ggccggacgc cctccttcag
     2821 agcctgcctg agcacggtct ccgtctgggg catggcgccc tctaccgcgt ccacgaccac
     2881 cacagcaccg tccactgcgc gcatggctcg ggtgacgtcg cccccgaagt ctacgtggcc
     2941 gggagtgtca atcatattga tcagatactc tttgccgtcc acctcgtgga ccatggagac
     3001 attggcggca tcgatggtga ttcccctggc ctgctccagg ggatcgaaat ccatgaatag
     3061 ctgctttcct gccagatcca tggagatcat gcctgccccg cccaacaggt tatcagaaag
     3121 ggttgtcttg ccgtgatcga tatgtgccac gattccgatg ttcctgataa actggggttt
     3181 atccatcaga gtcattacac gttctgctat cttcttcctc ttgcccataa tacgcctcgc
     3241 gaagtttgag ataaatctta taatcctgac taggactgag actcctcggg ctcaatgtat
     3301 ttaagccttc ggccccttct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 atagctctgg ttcggttttt gagatggcgc gcttggtaat gaagtttggt ggtgtttccg
     3481 ttgctgacgg aaaccggctg aggcatgtgg gcgatctggt cagacatttt catcgcgaca
     3541 atgagatagt cctcgtcacc tctgcccttc aaggggtaac ggatgagctt ctggcctgtg
     3601 ccagaagggc ggccagcgaa ggcaatgtct cagatgtgat ggacttcatg gagaggatca
     3661 cagatagaca caaccaagcg atagcagatg caatccagaa tcctgcaatc gccaaagagg
     3721 tacagggtat aatatccgag aagctctcat tgctcgagaa ggcctacatc ggcatctgct
     3781 atctgggaga gctaaccaga cgctcgctcg actatatctc cggctatggc gagcagctgg
     3841 ctgcacccat tctatccggg gtgctgcggg atatgggaat cgaatcccag cattataccg
     3901 gaagagaggc gggaatcatc accgacagca attacgggga tgccagaccc ctggagaaga
     3961 cctatggcct catcccccag aagctactgc cgttgaaggg cgtcccagtg gtcaccggat
     4021 tcatagccca ggacgagaag gggacgaaca ccacccttgg tcgtggtggc tccgacctct
     4081 ccgcatcact gatcggtgcg gcgatcaatg cggatgagat ctggttctgg aaggagacct
     4141 ccggcatcat gaccactgat cccaagatag tgccagaggc caagacgata cccaccatct
     4201 cctaccggga ggcgatggag ctctcctact ttggggctaa ggtcctccat cccagagcca
     4261 ttgagccggc catcaaaaag ggcatccctg tgagggttaa gtgcaccttc gatcccgagg
     4321 acccaggaac tcagatcgtg agggacgatg tccccaaaga ggatgtgatc aaggcggtca
     4381 cactgcacca gaaagtcgag cttctgaaca tctccggcgc gggaatgatt ggaactttag
     4441 gcgtcgctgc tcgcgccttc actgctttgg ccgaagcggg gatcaatatc gtgatgatca
     4501 gccagggctc ctctgaggcc aacatatcca tggtcataga ggagggacag gcagatcgag
     4561 cggatgaggc tctgcgatcc gagttcccca gagatgtggt caaggagatc tcccatgacc
     4621 atgatgtctg cacagtagcg gtgacgggct cgggaatggc cgggactcct ggggttgccg
     4681 cccgggtgtt caaggccatg ggtcaggcta agatcaatgt ggtcatgatc agccaggcat
     4741 ctggccagca taacatctcc tttgtggtag catcggcaga tggggaaaga gcagtccgag
     4801 agcttcatcg cgagttcggt ctgggtggtg aagcatgaag aagctcacct atgcccaggc
     4861 aggagtggac atcagcctgg agaataggtc catcgaggcc atgaagtccg ttctcaccac
     4921 caggcgcaaa gggttcgggg cgccattgac cgatataggc cattatgcag gcctcctgga
     4981 tatgggaagc tttgccctgg ccatgaccac agacggggtg ggttcaaagg ttctgatcgc
     5041 caatgccatc tccaagtggg ataccgtggg aattgactgc atagccatga atgtgaacga
     5101 cctgtatgcc atcggcgcgg agcccatagc ctttgtcgac tacctggcgg tggagaaggt
     5161 cgatcccgaa cgcgctgagc agatagccat aggtctcgcc cggggtgctg aactctccaa
     5221 tatgaccatc attggaggag agaccgcctc cctgccggag atcatccggg gattcgacct
     5281 ggccggaacc gctatcggcg tcgtggacaa ggataaggtg gtgaccgggg agaagataaa
     5341 gaagggggat gtgctggttg gggtccccag cagcggcctg cacagcaacg gctacactct
     5401 ggccagggcc attgtggccc aatccggata cacttacttc gatcgcctgc ccggagggga
     5461 aaaaagcata ggagaggagc ttttagttcc caccagaatc tatcatgaga tcgtagagct
     5521 ggtgggaagg tgcgatgtcc acggcctcgc gcacatcacc ggctcgggac tactaaagct
     5581 gcgccggatc agcaatctcg gattcgagat caccgatccg ctagaacccc agcccatctt
     5641 ccatttcctg caagagcagg gcagggtgga ggatctggag atgtaccaga cgttcaacat
     5701 gggaatgggt tttttggtag tgctggagga ggaagaggcc caggaagcct gccggatcat
     5761 gggacctggc tctcgagtca tagggtcggt ggtaggagag ggattaagag caggccatct
     5821 ggccttctag gcctgcctgt ttcatctatt tctcgatcta ttcattcttc tctattttat
     5881 tctcgccgaa catctcttca agcttctctt ttatcctctt gatggactgg atcgacgggc
     5941 agtcggcatc tataggaggc gtgcctgcca ggtccgcctc caccaggcag cggtcgaagg
     6001 gtatggttcc gaggactgga attcccattg ccgagagctt gtccgccaca ggacctgggt
     6061 tgtccgcctt gttgatgact gcaaagtacc tggagatgcc gatctctttg cccagctgca
     6121 ttatcctctc cacggtctca atggatctca tccccggctc caccacggcg atcatggcgt
     6181 cgacgcctct ggcagtagcc cggcccagat gctctatgcc cgcttccata tccaggatca
     6241 ccagatcctt ctcccgggtt atcacgtggc gaaggagggc tttgacgaag gcattggcag
     6301 gacacatgca gcccgatccg ccggagtcga tggtgcccat gaccaccagc ttgacgttgt
     6361 ccggcccggt gcagccgcac atg
//
LOCUS       DAOSGP010000050         6333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_10807_length_6333_cov_19.552724, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000050 DAOSGP010000000
VERSION     DAOSGP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10807_length_6333_cov_19.552724"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLV49_02565"
     CDS             complement(<1..881)
                     /locus_tag="PLV49_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG97755.1"
                     /translation="MMEEELEDRAALVTATIEKLLPVMHPRGLYEASRHLVDSGGKRL
                     RPSMLLLAAEAAGGEALALAPAAVSIELVHNFTLIHDDIMDNADVRRGRPAVHKIWGQ
                     SGAILAGDTLYSKAFQVLGMTAASPERILGAMNMLSRTCTAICEGQWLDMEFESKDRV
                     TENEYMEMIEKKTGVLYGASAGMGGLLAGASPEVVRALDEFGRLTGMGFQLQDDVIDL
                     LTPEEVSGKRQGGDLIEGKKTLIMIHAFANDVVVPVFSKKDASAEEIFRSISILEGSG
                     SIEYARSRAEEMVAQGK"
     gene            complement(927..>1543)
                     /locus_tag="PLV49_02570"
     CDS             complement(927..>1543)
                     /locus_tag="PLV49_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HOG97756.1"
                     /translation="VTTFSSHIARIRAIIEAAHKMGRKPVLLGRSMEKYWGTAVRTGY
                     AQQGEVSVFGRRKSVDKALKRIMNEGKDKYLPIMTGHQGEPGAILSRVANGETDFLVE
                     SGDRVIFSAGIIPQPLNESNRHTVVTKLKMRGGRIYDNVHVSGHACQEDHWELLRMIN
                     PEHVIPSHGTLRSHGSYLMMAEETGYSLGSNIHLVRNGQELLID"
     assembly_gap    1544..1643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1644..2092)
                     /locus_tag="PLV49_02575"
     CDS             complement(<1644..2092)
                     /locus_tag="PLV49_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG97757.1"
                     /translation="MKDIAIIAVGGYNEIGRNMTAIRIGKDIVVMDMGIRLDRVQIHE
                     DTDVESLHAADLINIGAIPDDSIMENVDGRVKAIACTHGHLDHIGAISKLAHKYHAPI
                     IASPFTADLINQEIKSEKIFGVNNPLKTMNAGERFQVTPDIDLEFVRV"
     gene            complement(2089..3186)
                     /gene="fni"
                     /locus_tag="PLV49_02580"
     CDS             complement(2089..3186)
                     /gene="fni"
                     /locus_tag="PLV49_02580"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOG97758.1"
                     /translation="MTTSSRKLDHIRICLDNPVESEGAAARSFDDLVLVHKALPEIDE
                     ADIDTSCRFLGRKLSAPLMISAMTGGHPSVKEINVNLALAASELGIAMGVGSQRAALE
                     EESLKDTFSAVRDAAPDIPIIGNIGAVQLKRSGPGILDQLAEMIDADAIAVHLNFLQE
                     SIQPEGDRDASGVVKVLGEAANGSVPIIVKETGAGISRETAASLADVGVKMIDVSGQG
                     GLSWAGVETYRAAEIGDGDLEEMGRLFWSWGIPTPVSIVECRSIGLDVISSGGIRSGL
                     DVAKSLSLGASLAGTALPMLKPATKDAKAVVRAMSPYLRALRICMFLTGCRRAEELKG
                     VPLVVLGRTREWLGARGYDLNEFSAYRELTK"
     gene            complement(3183..3935)
                     /locus_tag="PLV49_02585"
     CDS             complement(3183..3935)
                     /locus_tag="PLV49_02585"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HOG97759.1"
                     /translation="MVRVLKIGGSILTDKSCERGALPMEIERVAREVASRPHDLVLVH
                     GAGSFGHIPARQYGLPQRFSPEGLRVTHESVVRLNRLVLEALAKAGVEALPVHPLSCL
                     ILSGGRIESFATEPIAKMLEDGIMPILHGDVAMDLTQRAGIVSGDQLVSHLARALKAE
                     VVAVGCNVDGVLFSGRPMAEVKRSDLPLIEGAIGGSAGIDVTGGMKGKLDELLDLADM
                     GMSSMIFNASKEGNIVRALRGEAIGTKVWRPD"
     gene            complement(3952..4905)
                     /gene="mvk"
                     /locus_tag="PLV49_02590"
     CDS             complement(3952..4905)
                     /gene="mvk"
                     /locus_tag="PLV49_02590"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOG97760.1"
                     /translation="MTMASAPGKIILFGEHAVVSGTAALGGAIDLRARAIVQSLPGRV
                     LVETDDLSLRGFSLDLSTGEIRSASAAYATRYVSAVLKELDARDVRVMIESDIPLAAG
                     LGSSASIVVATVAALNGHLGLELSQKEIAALSYRIEKEVQKGRGSPMDTALATYGGYQ
                     RIADDNQRLDLPPLEMVVGYTRLPHDTFSLVEKVQLLKERYPDLVGPIFQAIGAISER
                     AAPLIREQRLKDLGELMDINHGLLEALGVGSRELSELVYAARNAGGALGAKLTGAGGG
                     GCMIALPGMAGKEALLVALRQARGMAFAAMMGCEGVRLEVA"
     gene            complement(4918..5706)
                     /locus_tag="PLV49_02595"
     CDS             complement(4918..5706)
                     /locus_tag="PLV49_02595"
                     /inference="COORDINATES: protein motif:HMM:NF001987.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMO1 family protein"
                     /protein_id="HOG97761.1"
                     /translation="MRPPAVAGQFYPGSSAELERQLDEFMPPAEEMPIVGGVVPHAGY
                     VYSGRIAAMVHSRLPKRETYVILGPNHHGLGSPVALSRDSWRTALGVVTPDLELADLL
                     TGSIIDHDEAAHQHEHSIEVQIPFLQKRFEDFKILAISMGMQDEDTAVEVGEEIARAA
                     LKLGRKCTVIASSDFTHYEPREVARKVDSQVIEAILNMDIPQIYSRVYRFNASSCGIG
                     PIAATIAASRLLGASSGKLLCYGTSGDVSGDYSQVVGYGAIVFT"
     gene            complement(5711..6331)
                     /gene="rpsB"
                     /locus_tag="PLV49_02600"
     CDS             complement(5711..6331)
                     /gene="rpsB"
                     /locus_tag="PLV49_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOG97762.1"
                     /translation="MEEDEVMTVAGEYETLIPIDEYLAAGVHIGTQQKTNDMMPYIYR
                     VRSDGLYVLDVQSTDKRIRLAAKFLSKYDQTNILIVSARQYGQRPALMFAKATGATAN
                     VGRFVPNTLTNPAFRGFIEPDVLIATDPSGDAQAVNEAVDVGIPVIALCDTNNMTSNV
                     DLVIPTNNKGRKALTLIYWLLARQVLREGGQEDRFKYTISDFEMEF"
BASE COUNT         1316 a         1998 c         1496 g         1423 t
ORIGIN      
        1 ctcttgccct gtgctaccat ctcctctgct ctggacctgg catactcaat cgagccgctc
       61 ccttccagaa tggagatcga ccggaagatc tcctctgccg atgcatcctt cttgctgaag
      121 acaggcacca ctacgtcatt ggcaaaggca tggatcatga tcaatgtctt cttaccctcg
      181 atcagatccc cgccctgcct ttttcccgag acctcctccg gggtcaaaag atcgatgaca
      241 tcatcctgga gctgaaatcc catgcccgtc agccggccaa actcatccag cgccctgacc
      301 acctcgggcg atgccccggc caggagaccc cccatccctg cagaagcgcc atagagaaca
      361 ccggtcttct tctcgatcat ctccatgtac tcgttctcag tcacccggtc tttagattcg
      421 aactccatgt ccagccactg gccctcgcaa atcgccgtgc aggtccggga gagcatattc
      481 atcgccccta agatccgctc cgggctggcc gcagtcatcc ccaaaacctg aaaggccttg
      541 gagtaaaggg tatcgccagc cagaatagct cccgactgcc cccagatctt atgcactgca
      601 ggcctccccc ggcgcacatc ggcattgtcc atgatgtcat catgaatgag agtgaagtta
      661 tggaccaatt cgatggatac cgccgccgga gccagggcga gagcctctcc acccgcagcc
      721 tcagccgcaa gtagcagcat cgacggcctc agcctcttcc cgccggagtc caccaggtgc
      781 cggctcgcct catacaggcc gcgcggatgc atcactggga gcagcttttc gatggttgcc
      841 gttaccagag ccgcacgatc ctccagctct tcctccatca tccttctcac aacctcccta
      901 tcccttgatt ctatttaaat taagcttcaa tcgatgagaa gctcctggcc gttcctcacc
      961 agatggatat ttgatcccag agagtagccc gtctcctcag ccatcatcag atagctgcca
     1021 tgactcctca gtgtcccgtg gctcggtatg acatgctccg gattgatcat ccgcaagagt
     1081 tcccagtgat cctcctggca ggcatgcccg gagacatgca cattatcgta gatcctgcct
     1141 cctctcatct ttagcttggt caccacagta tggcggttgg actcgttcag aggctgggga
     1201 atgattcctg cggagaagat cactcgatct ccggactcca ccagaaagtc ggtctctcca
     1261 ttggccaccc ggctcaatat cgcccccggc tccccctgat ggccggtcat gatgggcaga
     1321 tacttgtcct tgccctcgtt catgatcctc ttcagggcct tgtccaccga ctttctcctt
     1381 ccaaagactg agacttcccc ctgctgggca taaccggtcc gcactgccgt accccagtac
     1441 ttctccatgg accggcctaa gagcaccggc tttctcccca tcttatgagc agcctcgatt
     1501 atcgctctga tccgggcgat atgggaggag aaggtggtga ccannnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnacccgca cgaactccag atcgatatcc ggcgtaacct
     1681 gaaaacgctc tccagcgttc attgtcttca ggggattgtt caccccgaag atcttctccg
     1741 acttgatctc ctgatttatc aggtctgccg taaacggcga ggcaattatc ggagcatggt
     1801 acttatgagc cagcttggat atggctccga tatgatcgag gtgaccatga gtgcaggcga
     1861 tggccttaac ccttccatca acgttctcca ttatgctgtc atcgggaatg gcgcctatgt
     1921 taatcaggtc cgcggcatgc agggactcta catcggtatc ctcatgaatc tgcacccgat
     1981 ccagcctgat ccccatatcc attactacaa tatcttttcc tatccttatc gcagtcatgt
     2041 tgcggccgat ctcattatag ccgccgacgg cgattatcgc aatatctttc attttgtcaa
     2101 ctctctataa gcagaaaact cgttcaaatc ataaccccgg gccccaagcc attctctggt
     2161 ccttcctaga accacaagag ggactccttt cagctcctca gcccttctgc aaccggtgag
     2221 gaacatacag atcctcagag cgcgcagata cgggctcatg gcacggacca cggcttttgc
     2281 atcttttgtt gccggtttga gcatgggaag ggcagtcccg gcaaggctcg cccccagtga
     2341 gaggctctta gcaacatcca ggccgctcct gattcctccc gaggagatga cgtccaggcc
     2401 aatcgatcgg cactcgacga tgctcactgg agtaggaatt ccccagctcc aaaacagcct
     2461 tcccatctcc tccaggtcac cgtcgccgat ctcagctgcc cggtaggtct caactccggc
     2521 ccaggagagg cccccctgac cggagacgtc tatcatcttg actcccacat ctgccaggct
     2581 ggcagccgtc tccctggata tgcccgcccc cgtctccttg acgattatgg gcacggatcc
     2641 atttgctgcc tctcctaaca ccttgaccac tccggaggca tctcgatccc cctccggctg
     2701 aatgctctcc tggaggaagt tcagatgaac ggcgatagca tcggcatcta tcatctctgc
     2761 cagctgatcc aaaatcccgg gtccggaccg cttcagctgc accgcgccga tgtttccgat
     2821 gataggaata tcgggtgcgg catcccgtac cgcggaaaag gtatccttca ggctctcttc
     2881 ttcaagagct gccctctgtg agcccactcc catggctatg ccaagctcgg atgctgccag
     2941 agccagattt acgttgatct ccttgacgct gggatggcct ccggtcatag cgctgatcat
     3001 gagcggtgcc gatagctttc ttcccaaaaa ccggcaggaa gtatcgatat ctgcctcgtc
     3061 gatttccggc agagccttat ggacaagcac cagatcatca aaggatctgg ccgccgctcc
     3121 ctcagactcc acaggattgt ccagacagat tctgatatga tcgagcttcc gactggaggt
     3181 ggtcaatccg gcctccagac cttggtccct atggcctcgc cccgcagagc ccgcacgatg
     3241 ttcccctctt tggaggcatt gaatatcatt gagctcatgc ccatatctgc caggtctagt
     3301 aattcatcca gctttccctt catcccgccg gtgacgtcaa tacctgcgct tcctccgatg
     3361 gctccctcaa ttaacggaag atccgatctc ttgacctctg ccatgggccg gccagagaac
     3421 agtaccccat ccacattgca gcccacggcc acaacctctg ccttgagagc tctggccaga
     3481 tggcttacca actggtcccc ggatactatc cctgccctct gagtcaagtc catggccaca
     3541 tcaccatgca atatgggcat aatgccatcc tccagcatct ttgctatcgg ctcagtggca
     3601 aagctctcta ttcttcctcc ggacaggatc aagcaggata aggggtggac gggcagggcc
     3661 tcgacacctg ctttggcaag ggcctccagc accagacggt tcagtctgac caccgactca
     3721 tgggtcaccc tcaagccctc cggactgaac ctctgtggca ggccatattg tctggcagga
     3781 atatgtccaa atgaccctgc tccgtgcacc agcaccagat cgtgaggcct ggaggcgacc
     3841 tctctggcca cccgctcaat ctccatgggc agggcgcctc tctcgcagct cttatcggtc
     3901 agaatgcttc ctccgatctt caagacccga accattgctc gccctcccga ctcacgcaac
     3961 ctccagccgg acgccctcac agcccatcat agctgcaaat gccattcctc tggcctgcct
     4021 caaggccacc aataaagcct cctttccggc catcccggga agggcgatca tacagcctcc
     4081 tcctcccgct cccgtcagct tggcccctaa ggctcctccg gcattccttg cggcataaac
     4141 cagctcggac agctctctgg aacccacccc cagagcctcc agcaggccgt ggttgatgtc
     4201 catcagctca cccagatctt tcagtctctg ctctcggatc agaggagcag ccctctcgga
     4261 tatggctcct atggcctgga atatgggacc caccaggtct ggatagcgct cttttaagag
     4321 ctggaccttc tccaccaggg aaaaggtatc atgaggcagt ctggtgtatc ccaccaccat
     4381 ctctaaaggt ggaagatcga gacgctggtt gtcatctgca atcctctggt agccgccata
     4441 tgtggccaga gcagtatcca tggggctgcc tctccccttt tgaacctcct tctctatgcg
     4501 gtaggagaga gctgcaatct ctttttgtga taattccagg cccaggtgac cgtttagagc
     4561 agctaccgtg gcaaccacta tggacgccga tgatccgaga cctgctgcca gcgggatgtc
     4621 cgactcgatc atgaccctga catcccttgc atcaagctcc tttaaaacag cagatacata
     4681 tctggtagca taggccgcac tggctgatct gatctccccg gtagagagat ccagagaaaa
     4741 acctcgcaga gaaaggtcgt cagtctctac caaaaccctt cccggaaggc tctgcacaat
     4801 agccctggcc cgcagatcta tggctcctcc cagtgcggca gtgccggaga caacggcatg
     4861 ctctccgaag aggatgatct tgccaggagc cgatgccatc gtcatgagaa gccctcttca
     4921 ggtgaagaca atcgctccgt atcctactac ctggctatag tcgccgctca cgtccccact
     4981 tgtgccgtag caaagcagct tgccgctgct ggcccccaag aggcgagagg cggcaatggt
     5041 tgccgcaatc ggtccgatgc cacatgagga tgcattgaag cggtagaccc gggagtagat
     5101 ctgggggata tccatattca ggatggcctc aatgacctgt gaatccacct ttcttgccac
     5161 ttcccttggc tcataatggg taaaatcgct gcttgcaatc acggtgcact ttcgcccaag
     5221 cttcaaggcc gcccgggcga tctcctcgcc cacctctact gccgtatcct catcctgcat
     5281 tcccatggag atggcgagga tcttgaaatc ctcaaaccgt ttttgaagaa atggaatctg
     5341 cacctcaatt gagtgctcat gctggtgagc ggcctcatca tggtcgatga tgctccctgt
     5401 cagaagatcg gccagctcga ggtctggcgt gaccaccccc agagcagttc tccatgaatc
     5461 gcgagatagt gctaccggag atcctaagcc atgatggtta ggccccagga tcacataggt
     5521 ctctctcttt ggcaagcggg agtggaccat tgcggcaatc cggccggaat agacatatcc
     5581 ggcatggggg accacgccgc ccactattgg catctcctct gcaggaggca taaattcatc
     5641 cagctggcgc tcaagctccg ctgagctacc tgggtagaac tggccggcaa cagctggagg
     5701 cctcactgac tcagaactcc atctcgaagt ccgagatagt gtacttgaac cggtcctctt
     5761 gtccaccctc acgcagcacc tggcgagcaa ggagccagta gatcagagta agagcctttc
     5821 tgcccttgtt gttggtgggt attaccaggt cgacatttga ggtcatattg ttggtatcgc
     5881 acagggcgat cactggtatt cccacatcaa ccgcctcatt taccgcctga gcgtccccgc
     5941 tgggatcagt ggcaatgagc acatccggct ctatgaatcc ccgaaaggcc ggattggtca
     6001 gggtattggg cacgaagcgt cccacatttg ccgttgctcc tgtagccttg gcgaacatca
     6061 gggcaggcct ctggccgtac tgcctggcgg atacgatcag aatatttgtc tgatcgtatt
     6121 tggacaggaa cttggcggcc aggcggatgc gcttatctgt ggattgaaca tccaggacat
     6181 agagcccgtc tgaccttacc cggtaaatgt aaggcatcat atcatttgtc ttctgctgag
     6241 taccaatatg tacgcccgcg gcgaggtact catctatcgg tatgagagtc tcatattctc
     6301 ccgctaccgt catcacctca tcttcttcca act
//
LOCUS       DAOSGP010000051         6248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11012_length_6248_cov_18.287260, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000051 DAOSGP010000000
VERSION     DAOSGP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11012_length_6248_cov_18.287260"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /gene="guaA"
                     /locus_tag="PLV49_02605"
     CDS             <1..969
                     /gene="guaA"
                     /locus_tag="PLV49_02605"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOG97763.1"
                     /translation="RGDVVTVNLKSWDSGMLIMAMNAERFIENAIAEIKRDIKGRAII
                     GLSGGVDSSVCAMLAHRALGDRLVPVYVDSGLMRQSESDRIEELFKDLGLVRVNAQDR
                     FLEALKGVVDPEEKRMVVGETFIRVFEEEARRIGGDCLIQGTIYPDRIESDGGIKAHH
                     NVGGLPSRIEFKTIVEPLRDLYKDEVRQVARALGLSPEISERMPFPGPGLSVRIVGEV
                     TRDKLDVVRKANAIVEEEVEEFSPWQAFAAILGKATGVKGDNRVYGYVVAVRAVTSRD
                     AMTAEAMELPWQVLRRISGRITGEIPTVSRVVYDLTPKPPGTIEFE"
     gene            complement(974..1255)
                     /locus_tag="PLV49_02610"
     CDS             complement(974..1255)
                     /locus_tag="PLV49_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HOG97764.1"
                     /translation="MGALYHQFVGSPVGMRTASSLEKAIEESISLQPYVRRVEVRIDR
                     DMLSENVFGYGELEGRMIWALVEIEYEGEVISARLEYDRERCYPLMSLK"
     gene            complement(1279..1950)
                     /gene="pssA"
                     /locus_tag="PLV49_02615"
     CDS             complement(1279..1950)
                     /gene="pssA"
                     /locus_tag="PLV49_02615"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOG97765.1"
                     /translation="MNILRALRPPDLFTMLNIALGFAAILIICESQSLDFLKIAVVLI
                     ILAATADGLDGFVARKKGSGPLGANLDSLADLVSFGAAPAFLVVQAFSLPFQIWPAAI
                     FFVICGALRLARFNISNKSDQLFEGLPIPAAGMALSASVLLGRPGLTVTLMLILALLM
                     ISSIPYPKIRDKRVLSILGLFTIAAAWAVFFQSNTTYTAILLGALIIMYLLSPVVISR
                     LQKGK"
     gene            complement(1947..2612)
                     /locus_tag="PLV49_02620"
     CDS             complement(1947..2612)
                     /locus_tag="PLV49_02620"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOG97766.1"
                     /translation="MKLAKDSRGWISVPIILTAALAAAGNWYISAAAFMGLLFMIFFH
                     RDPDRLPQSEGMLSPADGRIIQATPEKVTVFMGFGDVHVNRSPLDGTIMSVEQRKGTH
                     LPAFLNRSCQNQQNRIRIDTEDGEIQLCQITGTFVREIICYVKPGDHVLRGERIGMIR
                     FGSRVETTIPRGYKLQVAIGDSVRAGKTVLAIKCNSELPGVPVASGSSGVLVSSGSSG
                     VTS"
     gene            complement(2609..3385)
                     /gene="artA"
                     /locus_tag="PLV49_02625"
     CDS             complement(2609..3385)
                     /gene="artA"
                     /locus_tag="PLV49_02625"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HOG97767.1"
                     /translation="MLDWLASYLLWISLLLLLVSSLTKKSLIALSGWLLFGVFWLNQP
                     GHYMAINDYFNAILFAAAGLMCFYMAWIMTRRSGPSKAQLWASYAAAVCGMVYFPFAE
                     IEPLREALIGFTTLMTVHALQALSVPVSMPSWNIMTLNGRSVEIILACTAIESIALFS
                     GLIISVRAPIGRRAAALAVSIISIYILNLVRNGFVIMAYGWSWFGSASFEVAHNLIAK
                     IGSVAALLLISYMIFLLLPELLSIIDELAADIRHPRGDAA"
     gene            3493..>6248
                     /locus_tag="PLV49_02630"
     CDS             3493..>6248
                     /locus_tag="PLV49_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOG97768.1"
                     /translation="MDRNAFILFSAASVLLLAVAGAQIAEAGSSDAVGKIDTDLAEML
                     DGAGEERIPVIVMLKGTEAPDFGGFDVKYSYQLISGLAGEASSTTIREMAESDRVSAI
                     YFDDPAKISAPGEDNASAPDNALVYDNSSVDGQDNGSDNTSIGYISPARIIKADKLWE
                     MGIDGRGVNVAVIDSGIDKNHPDLIGKVIAEKNFLANEITADDLLGHGTMIAGIIAGS
                     GASSGGRYVGIAPGASLINVKVIDQNGDGRVSDIIAGIEWAIYNGADVLSLSLGGINL
                     GETNPPITMAADNAADQGVVVCVAAGNRNSTENENQLSGASSSQLRGSPVELSQTEES
                     SGKDVYFLFVPIVFALPPGLIDSPGDGVNVITLGSTDAAGHMASFSGSGPTRDDRIKP
                     DVVAPGMDIISTVPAGLEGPKYVDTYYARESGTSLSTPIAAGLAALLLQERSNLTPAG
                     IKGAMTRGAAKLNNTHGVAYESYYQGTGLLDALHSYQLLEELGDGMSGVVPDLWKPGR
                     WAYLPSGEGVYVGLDTGADRPQKKVYALAPGDRDWNTRFVFFSNRERDGISLSAQGEI
                     ADWVSLQALPEHISSNSQHVFAASIQVPEDADPGDYSGSLDITDSGETVLEIPVSLTV
                     AEPLSISQGRGENSGSLTGNQWDYYYLDLPVGSRGIEVRLDWNSESDSVDADRTDSNS
                     TASFSTNASSTDLGSSLDLFLISPTSEYYAGGPEGSSKRVAIESPPSGRWLVAVYSEN
                     ASVNASYTLQVEQEMIEPTPKRWHIDSANPGMNASIEILLENKGQTMENLSYAAVIEN
                     ESLVEYEDRVGLKQIWNQTVNVTDETIRLSAELATIDGSRYSEIALVFEDPDGMASEE
                     HADLGSGYLGPVEVSNPQAGRWAINVYGYNVPETGQPFRLTVKQYKEEPWSWISISGP
                     DRLEA"
BASE COUNT         1453 a         1623 c         1851 g         1321 t
ORIGIN      
        1 agaggagatg tcgtcaccgt aaacctcaaa tcttgggact cgggcatgct aatcatggcc
       61 atgaatgcgg agcgtttcat tgagaacgcc attgcagaga tcaagcgcga cattaagggt
      121 cgcgctatta tcggtttatc tgggggcgta gacagctcgg tatgcgctat gcttgcccat
      181 cgggcattgg gtgaccggct ggttcctgtc tatgtggact ccggtctgat gaggcagtcc
      241 gagtccgatc gaattgagga actattcaag gatctgggcc tggtgcgggt caatgcccag
      301 gaccgatttc tagaggctct taaaggggtt gttgatcccg aagagaagag gatggtggtg
      361 ggagagacat tcatccgggt tttcgaggaa gaggcgcgca ggatcggcgg cgattgcctc
      421 attcagggca ccatctatcc ggacagaatc gaatcggatg gaggcatcaa agcccaccac
      481 aatgtaggag gcctccccag ccggatcgag ttcaagacca tagtcgagcc tctgcgcgac
      541 ctgtacaagg acgaggtccg ccaggtggca agggctctgg gcctctcccc tgagatcagc
      601 gaaaggatgc cttttcccgg tcctgggctg tcggtcagga tcgtgggaga ggtcactcgg
      661 gacaagctgg atgtggtcag aaaggcgaac gccattgttg aggaggaggt ggaagagttc
      721 tctccctggc aggcatttgc cgccatcctg ggaaaggcga ccggggtcaa gggagacaac
      781 cgggtctatg gctatgtggt cgccgtccgc gcagtcacca gccgcgatgc catgaccgca
      841 gaggcgatgg agcttccctg gcaggttctc aggcgcatct ctggccggat caccggggag
      901 atcccgacgg tctccagggt ggtctacgac ctgaccccaa aaccgccggg cactatagag
      961 tttgagtaat ggcctatttc aggctcataa ggggatagca cctctcccgg tcgtactcca
     1021 gccgggcgga gatcacctcc ccttcatact ctatctccac cagggcccag atcatcctcc
     1081 cttccagctc gccgtagcca aagacattct ctgagagcat atcccggtct atcctcacct
     1141 cgaccctccg cacatagggc tgaagggaga tgctctcctc tatggccttc tccaggctgg
     1201 atgctgtcct cattcccact ggcgagccca cgaactggtg atagagtgct cccagcttta
     1261 ttcctgcctc aaaggctgct atttcccttt ttgtaggcgt gaaatcacca ccggacttaa
     1321 caaatacatg attatcagag cgcccaacag gatggcggta tatgtggtat tgctttgaaa
     1381 aaagaccgcc caggcggcag caatggtgaa gaggcccaat atagacagga ctctcttgtc
     1441 ccttatcttg ggatatggta tgctgcttat catgagcaaa gccaaaatga gcatgagtgt
     1501 aacggtaagc ccaggccgcc ccaataggac gctggctgat agagccatgc ctgcagcagg
     1561 aatgggcagg ccctcgaaga gctgatcgct cttgtttgag atattgaacc tggccaggcg
     1621 gagggctcca cagatgacaa agaagatcgc tgccggccag atctggaagg ggaggctgaa
     1681 ggcctgaaca acaagaaagg ccggcgctgc accaaaggac accaggtctg ccagggagtc
     1741 caggttagcc cccaggggac cggatccctt ctttctggct acaaatccgt ccaggccatc
     1801 tgctgtcgct gctaatatta tgagaacgac tgcgatcttt aaaaaatcca gagattgcga
     1861 ttcgcagatg atgaggatgg cagcaaatcc cagagcgatg ttcaacatgg tgaacaggtc
     1921 tggcggccgg agggcgcgca ggatattcat gaagtgaccc ctgatgaccc agaagagact
     1981 agaactcctg atgaacctga tgccactgga actcctggca gctccgagtt gcacttgatg
     2041 gcaagaaccg tcttcccggc ccggacgcta tctccaatgg ccacttgtag cttatatccc
     2101 ctgggaatgg tggtttccac ccgcgagcca aagcggatca tgcctatcct ctccccccgg
     2161 aggacgtgat ctccgggttt gacataacag atgatctctc ttacaaaggt cccggtgatc
     2221 tggcacagct gaatctctcc gtcctcggta tcgattctaa ttctgttttg ctggttttgg
     2281 cacgagcggt ttagaaaggc tgggaggtga gtgcctttcc tttgctcgac gctcattatc
     2341 gtcccatcta aaggcgaccg gttaacgtgc acatcgccga agcccatgaa tacggtgacc
     2401 ttctcagggg tagcctgaat gatccttccg tctgctggag agagcatacc ctcgctttgc
     2461 ggcagacggt ctgggtcccg gtgaaagaat atcatgaaca gcaaacccat aaaagcggca
     2521 gccgatatgt accagttccc ggcagcggcc agggcagcgg tcaggataat gggcaccgat
     2581 atccagccgc gggagtcttt tgccagcttc aagcggcatc tcctctgggg tgtcttatgt
     2641 ccgctgccag ctcatctatg atcgagagga gctcggggag gagcaggaat atcatatagg
     2701 atatcagcaa gagagcagca acagatccta tcttggcgat cagattatgg gcaacctcaa
     2761 agcttgcgct tccaaaccag ctccagccgt aggccatgat cacgaatcca tttctcacta
     2821 ggttcaggat atagatggag atgatggaga ccgcaagagc tgccgccctg cggccgattg
     2881 gtgctcggac ggatatgatg agtcctgaaa agagggcgat gctctcaatg gctgtgcagg
     2941 cgaggatgat ctccaccgac ctgccgttca gagtcatgat attccaactg ggcatgctca
     3001 ccggaacaga tagagcctga agggcgtgca cagtcatgag ggtggtaaac cctatcagtg
     3061 cctctctcag gggctcgatc tcggcgaatg gaaaatagac cattccgcat actgcagcgg
     3121 catagctcgc ccagagctgg gctttggagg ggccgcttct tcgagtcatt atccaggcca
     3181 tgtaaaagca catgagaccg gcagcagcga agaggatggc attgaagtag tcattgatgg
     3241 ccatatagtg tccaggctga ttcagccaga atacgccaaa taggagccat ccggacaggg
     3301 cgatgaggct ctttttggtc agggatgata caagcagcag aagcaagctg atccagagca
     3361 gataagaagc cagccagtcc aacatcattc acctaagaag taccacaggt ttatttagca
     3421 ttatctactt acaacgcgat gatatgaatt tggtgggggc tggattcaca ccatttatag
     3481 gtttggttga tcatggatcg aaatgctttt attttatttt ctgcagcgag cgtgcttctc
     3541 ctggccgtgg ccggagcgca gatagctgaa gcagggtcat ctgatgccgt cggaaagata
     3601 gatactgatc tggcagagat gctcgatggt gcgggggagg aaaggatacc tgtgattgtg
     3661 atgctgaagg gcaccgaggc tccggatttt ggcggctttg atgtcaagta cagctaccag
     3721 cttatctctg gcctggcagg agaggcaagc agcactacca tccgagagat ggcagagagc
     3781 gatagagtat ccgctatcta tttcgatgat cctgcgaaga tatcggcacc aggcgaggat
     3841 aatgcatctg caccggataa tgctctggtt tatgataact cttccgtcga tgggcaggat
     3901 aacggttctg ataatacgtc tattggttac atctctcctg cccggatcat caaagcagac
     3961 aagctatggg agatggggat cgatggccgg ggggtaaacg ttgcggtgat cgattcaggc
     4021 atcgataaga atcatcccga tctgataggc aaggtgatcg ctgagaagaa cttcctggcc
     4081 aatgagatca cggcagacga tcttttgggt catggcacaa tgatcgccgg gatcatcgcc
     4141 ggctcaggtg ccagctcggg cggtagatat gtgggaattg cccccggggc gagcctgatc
     4201 aatgtcaagg tgatcgacca gaatggcgac ggcagggtat cggatatcat cgccggcatt
     4261 gagtgggcca tctacaatgg cgctgacgtt ctgagcctca gcctgggagg gataaacctg
     4321 ggcgagacca atccccccat aaccatggcc gcagataatg ccgcggatca gggtgtggtg
     4381 gtatgtgtgg ctgcgggaaa ccgcaacagc actgagaacg agaatcagct gtcgggagca
     4441 tcgtccagcc agcttcgggg ctctccggtc gagctctccc agacggaaga gagcagcggc
     4501 aaggacgtct atttcctgtt tgtgcccatc gtcttcgccc tgcctccggg gctgatcgac
     4561 tctccaggcg acggggtgaa tgtcatcacc ctgggttcaa ccgatgctgc ggggcatatg
     4621 gccagcttct ccggctccgg accgaccagg gacgacagga tcaagccgga tgtcgtcgct
     4681 ccgggaatgg atatcatctc cacagtgcct gctggccttg aggggccgaa gtatgttgat
     4741 acttattatg cccgggaatc ggggacaagc ctctccactc ccattgccgc aggtctcgct
     4801 gcccttctct tgcaggagag gagcaaccta actcccgctg ggatcaaggg ggcgatgacc
     4861 aggggagcgg ccaagctcaa caacacccat ggggtggctt atgagtcata ctatcaggga
     4921 actggccttt tagatgctct ccattcttac cagctcctgg aggagctggg agatgggatg
     4981 agcggcgttg tgcctgacct gtggaagccg ggtcggtggg catatctgcc ctcaggagaa
     5041 ggggtctatg tgggcctgga tacaggagca gaccggccgc agaagaaggt ctatgccctt
     5101 gctcctggag accgcgactg gaataccagg ttcgtgttct tcagcaaccg ggagagagat
     5161 gggatttccc tctccgctca gggggagata gcagattggg tcagcctgca ggcccttcct
     5221 gagcatatat catccaacag tcagcacgtc tttgccgcat ccatccaggt gccggaagat
     5281 gcggatcctg gagattattc cgggtcgctt gatatcaccg actccggaga gacggtcctt
     5341 gagataccgg tcagcctgac ggtggcagag cccctatcca tctcccaggg gagaggagag
     5401 aattccggaa gcctgaccgg gaaccagtgg gattactatt atctggattt gccggtggga
     5461 tcgaggggga ttgaggtcag gctggactgg aactccgaat cagattccgt tgatgctgat
     5521 cgaactgact ccaactcaac ggcttccttc tccaccaacg ccagctccac ggaccttggc
     5581 tccagcctgg acctgttcct gatatccccc accagcgagt actatgctgg agggccggag
     5641 ggcagctcca agagggtagc aatcgagagc ccgccttcgg gaaggtggct cgtggccgtg
     5701 tatagcgaga atgcatccgt taatgccagc tataccctcc aggtggagca ggagatgata
     5761 gagcccactc ccaagcggtg gcacatagac tccgccaatc cgggaatgaa tgccagcatc
     5821 gaaatcctgc tggagaacaa aggccagacg atggagaatc tgagctatgc ggcagttatc
     5881 gagaacgaaa gcctggtgga gtatgaggat agagtgggat tgaagcagat ctggaaccag
     5941 acggtgaatg taaccgatga gaccataagg ctctctgcag aacttgccac catcgacggc
     6001 agcagataca gcgagatagc cctggtcttt gaggatcccg atggaatggc aagcgaggag
     6061 catgcggatt tggggtcggg atatctcggt ccggtggagg tctccaatcc ccaggctggc
     6121 aggtgggcga tcaacgtcta tgggtataat gtccccgaaa ccggccagcc cttcaggctc
     6181 actgtcaaac aatacaaaga ggagccctgg agctggatca gcatcagcgg ccctgaccgg
     6241 ctggaggc
//
LOCUS       DAOSGP010000052         6238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11041_length_6238_cov_19.283681, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000052 DAOSGP010000000
VERSION     DAOSGP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11041_length_6238_cov_19.283681"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1751)
                     /gene="alaS"
                     /locus_tag="PLV49_02635"
     CDS             complement(<1..1751)
                     /gene="alaS"
                     /locus_tag="PLV49_02635"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG97769.1"
                     /translation="MFTEDEYRLDFFIEEGFHHKKCEKCGKFFWTRDGSRKTCGDPPC
                     DPYTFIGSPIFKRQHSLDEMREHYLGFFEARGHTRIKRYPVAARWRDDIYLTIASIAD
                     FQPFVTSGQVPPPANPLTISQPCIRLDDLDSVGRSGRHLTTFEMMAHHVFNTPDREIY
                     WKDRTVRLCDELLVGLGMDPLAVTYKENPWAGGGNAGPSVEVMVGGLELATLVFMDLV
                     AAPAGAVEIKGERYEKMKNYIVDTGYGLERFVWASNGAPTIYDAIFPDLVRQVADLAG
                     VEHNLDDPEYAEIFAQNARLAGMVDLDEYSMSDLRAKIAGSIGIDPERLEKAILPMER
                     VYAVVDHTRCLAYMLGDGIIPSNVKGGYLSRLVIRRTLRLMKELGLRLPLADLVQLQI
                     SRLDYPDWEESFATIREILDQEEQKYAETLEKGMRLVRKTAESYLKKNEPVPLSEMIA
                     LYDTHGIPPEIAREAAVQMGAQVELPDNFYSLVAKKHIRAEPVDEKKPPIPGKTELLF
                     YENPFEQQFDAEVLDVVEGAAILDRTLLYPEGGGQPADHGTLEKDGQLFQVTDVQKSG
                     DVVLHKLAEPCCLAKGDR"
     gene            complement(1850..2467)
                     /locus_tag="PLV49_02640"
     CDS             complement(1850..2467)
                     /locus_tag="PLV49_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HOG97770.1"
                     /translation="MKNIEDLMKKATELKAEGLVEGQISEELNVSGETVTWLLTHAEK
                     TSTSPGPKDISVDWSAIGRSAFRLNHISEAMTDIIYETLDANESSVDVVVGIALSGLP
                     IASMVANDLAAELAVYTPSKQMIAQENKRAKGNLSSNFSDVQDKDCIIVDDVVTSGQT
                     LEEAVEYLDEHGARINAIAVLIDKKGIEEIAGVPVVSLLKVMRVN"
     gene            complement(2464..2916)
                     /locus_tag="PLV49_02645"
     CDS             complement(2464..2916)
                     /locus_tag="PLV49_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOB1 family endonuclease"
                     /protein_id="HOG97771.1"
                     /translation="MTVADASVFIWGKRPEGELITVPAVEAELKDIRARSRLHIYETR
                     VESPTTIALKAARDAAEQTGDIRSLSAADLEVLAKALEYNATLATDDYALQNVALHLG
                     LKIEPIGQPRIKKKRKYVQRCQGCGQRFEGEACPDCGTPARKKKRCMR"
     gene            3004..3438
                     /locus_tag="PLV49_02650"
     CDS             3004..3438
                     /locus_tag="PLV49_02650"
                     /inference="COORDINATES: protein motif:HMM:NF039857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97772.1"
                     /translation="MAKKEKKSGRKDERGLAAEQNKMTAPKEAETKTKKEPARPKTAA
                     DRKKDRRDGIVKTVFAAALGVLAGFACYYIASTTGENPWEIGEFPWHFILLSVILVTS
                     LFQRVAYPLLKIDAASFKTKDWLYVEFIVVDLWLVTWTILLN"
     gene            3447..5207
                     /locus_tag="PLV49_02655"
     CDS             3447..5207
                     /locus_tag="PLV49_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HOG97773.1"
                     /translation="MRIAIINRDRCQPRKCSKECEYFCPPVRTGDETIVFADGKPLIT
                     ENLCVGCGICVRKCPFGAITITNLPEEMEDPVHRYGQNGFALYGLPVPIEGRVTGLLG
                     PNGIGKSTAISILSGILRPNLGKDASWEEALQRFSGSILGDYLKKVVDKGIKTSYKPQ
                     YVDRIPKSYSGMVSGLLEKTDERGALAELTGTLGISKLMDRDISSLSGGELQRVAIAA
                     AAAKDADFYFFDEISPYLDIHQRINSARILQSLAKDKAVMVVEHDLALLDLLAENVHL
                     IYGVPGAYGVITRPKGVRVGINQYLRGNLPEENVRFRDTAINFEVHAPRIEKEIPSLI
                     AYDAFAMKYSSFRLEAEAGIIRRGEVVGILGPNGIGKSTYIKILAGVEKPTTGSFESQ
                     LKISYKPQYLKADTELTVEGMLRGLTHGFDSSYYQAEILRPMGIEPLLDKILSELSGG
                     ELQRVAITACLSREADLYLLDEPSAHLDVEQRMLAAKVMRRFAESTEKTVLVVDHDIY
                     LIDLLSERLMVFDGVPGILGVAHTPLEMREGMNAFLKGIGITFRRDEETRRPRVNKPE
                     SRLDRMQKEQGEYYYQIEEA"
     gene            5220..>6238
                     /locus_tag="PLV49_02660"
     CDS             5220..>6238
                     /locus_tag="PLV49_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97774.1"
                     /translation="MREFIRRNNPFNRHFVRNLEAGTLLEIFLVTAVCSVLGIRFFLA
                     LTGYPSISPGHLHIAHVLLGGVLMMIALVINLGYINKSANYLVAVIGGLGFGAFIDEL
                     GKFITGDNDYFFQPTVALIYIIFALLYLAIQTFIQKPKLTEQERLINVLELAKEAVLE
                     DLDHLERRKALALLKESSQNNPMTRALQDLLHSTDSVPMPKIDIYTATKYRMRRYYRR
                     LIKKSWFIRVVMAFFILQSIFTLGLDFMLIYLRLNSGKGLDAIFPSLSFFDLAGLAAA
                     TLSSILVIYAIIKMKSSRLEAYRIFKNAILVQIFIVQVILFYLTQFIALLGLAGNICI
                     LLVLN"
BASE COUNT         1388 a         1780 c         1671 g         1399 t
ORIGIN      
        1 cgatcccctt tggccaggca gcagggctcg gccagcttat gcaagaccac atcccctgac
       61 ttctggacgt ctgttacctg gaacagctgc ccatccttct ccagggtgcc atgatcagca
      121 ggctggcctc caccctccgg gtagagaagc gtccggtcca ggatggccgc cccctccacc
      181 acatccagaa cttctgcatc gaactgctgc tcgaatggat tctcatagaa gagcagctcc
      241 gtcttgcccg gtatgggggg ttttttctca tccaccggct ccgctcggat gtgctttttt
      301 gcgaccagtg aatagaagtt gtccggcagc tccacctgag ctcccatctg caccgctgcc
      361 tcgcgggcga tctccggagg gatgccgtgc gtatcataga gggcgatcat ctccgagagg
      421 ggcacaggct cattcttctt cagatagctt tctgccgtct ttcgaaccag gcgcatgccc
      481 ttctccaggg tctcggcata cttctgctcc tcctggtcca ggatctccct gatggtagcg
      541 aaagactcct cccagtctgg ataatccagg cgtgagatct gaagctggac caggtcggcc
      601 aggggcagcc tcaggcccag ctctttcatc agccgcagag tgcggcgaat taccagccgg
      661 gagaggtagc cgcccttaac attggagggg atgataccgt ctcccagcat ataggccagg
      721 cagcgggtgt gatcgaccac cgcatagacc cgctccatgg gaagaatggc cttctccagc
      781 cgctcgggat caatgcctat gctgcctgcg atcttcgccc gcaggtcaga catactgtac
      841 tcgtccaggt ccaccattcc agccaggcgg gcgttctggg cgaagatctc cgcatactcc
      901 gggtcatcca ggttgtgctc tacgcccgcc aggtctgcca cctgcctgac cagatcgggg
      961 aatatggcgt catagatagt gggagcacca ttagaggccc agacgaacct ctccaggccg
     1021 tagccggtgt caacgatata gtttttcatc ttttcatagc gctcgccctt gatctcgaca
     1081 gcgcccgcag gcgcagccac cagatccatg aacaccaggg tggccagctc caggccaccc
     1141 accataacct ccacgctcgg cccggcgttc cccccaccgg cccagggatt ctccttgtag
     1201 gtcaccgcca gggggtccat ccccaggcca acgagcagct cgtcacaaag ccgcacagtc
     1261 cggtccttcc agtagatctc cctatcaggg gtgttgaata cgtggtgggc catcatctca
     1321 aaggtggtga gatgccgccc gctccgcccc actgaatcca ggtcgtctaa acgaatgcag
     1381 ggctggctga tggtcagcgg attggctggc ggcggtacct gccctgaggt aacaaaaggc
     1441 tgaaaatcgg caatggaagc aatggtcaga tagatgtcat ctcgccacct ggccgccacc
     1501 ggatacctct tgatccgggt atgcccccgg gcctcgaaga agcccagata atgctcgcgc
     1561 atttcatcca ggctatgctg gcgcttgaat atgggagagc caatgaaggt atagggatcg
     1621 cagggcggat cgccgcaggt cttcctgctc ccgtccctgg tccagaagaa tttgccgcat
     1681 ttctcgcact tcttatgatg gaagccttcc tcaatgaaga agtccaatcg atattcgtcc
     1741 tcggtgaaca tggcacatca gcttttgccg ggagaagggc gaggattgct aaaaggtttt
     1801 ccaaatggcc aggcattgct gaccatcaga atatctctta atctcgcccc tagttcaccc
     1861 tcatgacctt cagcagggaa accactggca ctccggcgat ctcttcaatg cctttcttgt
     1921 cgatcaggac ggcgatggca ttgatccttg ccccatgctc atccagatat tccactgctt
     1981 cctccagggt ctgaccggat gtcaccacat catcgacgat gatgcagtcc ttgtcctgga
     2041 catcggagaa gttggagctc aggttgccct tggccctctt gttctcctgg gcgatcatct
     2101 gtttgctggg ggtgtaaact gccagctccg ctgccaggtc gtttgccacc atgctggcaa
     2161 ttggcaggcc gctcaaggcg attcccacca ccacatccac gctgctctca ttggcatcca
     2221 gggtctcata gatgatgtcg gtcatggcct cggatatgtg gttgagcctg aaggcgcttc
     2281 ggcctatagc cgaccagtcc accgatatat ccttggggcc gggggaggtg ctcgtcttct
     2341 cggcatgggt taaaagccag gtcaccgtct ctccggatac attcagctcc tctgagatct
     2401 gaccctctac cagtccttct gccttaagtt ccgttgcctt cttcatcaaa tcttcaatat
     2461 ttttcatctc atacaccttt tcttctttcg ggcgggagtt ccgcaatcag gacatgcttc
     2521 cccctcgaat ctctggccgc agccctggca cctctggaca tatttgcgct ttttctttat
     2581 ccggggctgg ccgatgggct cgatcttcag gccgaggtga agagcgacgt tttgcagagc
     2641 atagtcgtcg gtagcgagag tagcattata ctccagtgcc ttggccagga cctccagatc
     2701 ggccgcagag agagaacgaa tgtcccccgt ctgttcagcc gcatctctgg ccgctttcag
     2761 ggcaatagtg gtggggctct ccacccgggt ctcatatata tggagccggg accttgctct
     2821 tatatccttt agctccgcct ccaccgccgg gacggtgatc agctcccctt ctggcctctt
     2881 gccccagatg aacaccgaag cgtccgctac ggtcatacac atgcaattgc tcttcttgtt
     2941 tatagtactt ggacttttcc cggaccaaaa cgctattatc atataatgag gtctggaaat
     3001 ccgatggcca agaaagagaa aaagtctggc agaaaggatg agcgagggct ggcagcggaa
     3061 cagaataaga tgacggcgcc aaaggaagcg gagacgaaaa cgaaaaaaga accggccagg
     3121 cctaagacag ctgccgatag gaagaaagac cgccgggatg ggatagtgaa gacagtgttc
     3181 gccgccgcac tgggagtcct ggcaggcttt gcctgctact atattgccag caccacgggc
     3241 gagaatccat gggagatagg cgaattcccc tggcatttca tattgctatc agtgatcctg
     3301 gtcacctctc tattccagag ggtcgcctat cccctgctga agatagatgc agccagcttc
     3361 aagaccaaag actggctata cgtggagttc attgtagttg acctctggct ggtcacatgg
     3421 acaatactac tcaattgagg tagaacatga ggatagctat catcaatcgc gaccgatgcc
     3481 agccgagaaa atgctctaaa gaatgcgaat acttctgccc gcccgtccgc acgggcgacg
     3541 agaccatcgt gtttgcagat ggcaagcccc tcattactga gaacctctgt gttggctgtg
     3601 gaatatgcgt acgcaaatgc ccctttggag ccatcaccat caccaatctg cccgaggaga
     3661 tggaggatcc agttcataga tacggccaga acggctttgc cctttatggc ttgcctgtgc
     3721 cgatcgaggg aagggtcact ggtcttttgg gccccaacgg cataggaaag agcacggcga
     3781 tatccattct ctccggtatt ctccggccga acctgggcaa agacgcatcc tgggaggagg
     3841 cgctacagcg cttctccggc tccatcctgg gagactatct gaagaaggtg gtggataagg
     3901 ggatcaagac ctcttataag cctcagtatg ttgatcgcat cccaaagagc tattcaggca
     3961 tggtttcggg cctcttggaa aagactgatg agaggggcgc cctggccgaa ctgacgggga
     4021 ctttgggcat atcaaagctc atggacagag acatctccag cctcagcggc ggcgagctgc
     4081 agagggtggc catcgcagct gcggcggcaa aagacgctga tttctacttc ttcgatgaga
     4141 tcagcccgta ccttgatatc caccagcgca tcaactccgc ccgcatactg cagagcctgg
     4201 ccaaagataa ggcggtgatg gtggttgagc acgacctggc cctgcttgac ctgctggcgg
     4261 aaaacgttca tctcatctat ggcgttcccg gtgcttatgg cgtgataacc aggcccaaag
     4321 gggtgcgggt gggcatcaac cagtaccttc gcggcaacct gcccgaggag aatgttcgct
     4381 ttcgcgacac cgccatcaac ttcgaggtac atgccccccg aatagagaag gagatcccat
     4441 ccctcattgc ctatgacgct tttgccatga agtattcttc cttccggctg gaggccgaag
     4501 cggggatcat ccgccggggc gaggtggtgg ggatcttggg gcccaatgga attgggaaga
     4561 gcacttacat caagatcctg gcaggagtgg aaaagccaac aactggcagc tttgaaagcc
     4621 agctgaagat atcctataag ccccagtacc tcaaagcaga cactgaattg accgtagagg
     4681 gcatgctgag gggattgacg cacggatttg acagcagcta ctatcaggca gagatcctca
     4741 ggcccatggg catagaaccc ctcttagata agatcctctc tgagctctca ggcggggagc
     4801 tgcagagagt ggccataact gcctgcctca gccgggaggc ggacctctat ctactggacg
     4861 agccttccgc ccatctggac gtggagcaga ggatgctggc cgccaaggtc atgagaaggt
     4921 tcgccgagtc cactgagaag actgtactgg tggtggatca tgacatctat ctgatcgatc
     4981 tgctcagcga gcgcctgatg gtcttcgatg gcgttcctgg aatcctcggt gtggcccata
     5041 ccccgctgga gatgcgcgag gggatgaacg ccttcctcaa agggatcggc ataaccttcc
     5101 ggcgcgatga agagacccgc aggcccagag tcaacaagcc ggaatccagg ctggacagaa
     5161 tgcaaaaaga gcagggagaa tattattatc agattgaaga agcatgaagt gaatggagaa
     5221 tgagagaatt catccgcagg aataatccat tcaaccgcca tttcgtgcgc aacctggaag
     5281 cgggaacact gctggagata ttcctggtaa cagcggtatg ctccgtcctg ggaatacgct
     5341 tctttctcgc cctaacagga tatccgagca ttagcccggg gcatttgcat atcgcccatg
     5401 tcctcttggg cggcgtgctg atgatgattg ctctggtcat caatctgggc tacatcaaca
     5461 aatcagccaa ctatcttgtg gcggtgatcg gaggcctggg atttggcgca ttcatcgatg
     5521 agctgggcaa gttcatcacc ggggataatg attacttttt tcagcccact gtggcgctga
     5581 tctacataat attcgccctt ttgtacctgg caatccagac cttcatccaa aagccgaagc
     5641 tcaccgagca ggagaggctg ataaatgtcc tggagctggc aaaagaagct gtcttggagg
     5701 atctggatca tctggagagg agaaaagcgc tggctctgct aaaggagagc tcccaaaata
     5761 atccaatgac ccgagccctg caggatcttc tccacagcac cgatagcgtg cccatgccca
     5821 agatcgacat ctacaccgcc acgaagtatc gtatgcgcag gtactaccgg aggctgatca
     5881 aaaagagctg gtttataaga gtggttatgg ccttcttcat cctccagtcc atcttcaccc
     5941 tgggccttga cttcatgctc atttacctca ggcttaactc ggggaagggt ctggatgcca
     6001 tatttcccag cctatcgttc ttcgatctgg ccggactggc tgcagccacc ctatcctcca
     6061 tcctggtcat ctatgccatt ataaagatga aaagctcccg cctggaggcc tacaggatat
     6121 tcaagaacgc cattcttgtg cagatattca tagtgcaggt catcctcttc tatctgacac
     6181 aatttattgc cctgctgggc ctggccggca atatctgcat acttttagtg ctcaacta
//
LOCUS       DAOSGP010000053         6172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11181_length_6172_cov_16.224456, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000053 DAOSGP010000000
VERSION     DAOSGP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11181_length_6172_cov_16.224456"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1508
                     /locus_tag="PLV49_02665"
     CDS             246..1508
                     /locus_tag="PLV49_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG97775.1"
                     /translation="MIYALVILSPTNAADFVLEIYGNANMDEYLDQADLDYIRDIVAG
                     KTEPTKLADANFDGQVNEQDVEQLQAILDGREEKLTFIDMLGEPVTVNKPIRRLVNMG
                     YNGVEMTRILGAEDILVAYGQDRADHAIFFPKFAELPFVGSDPNDCDYEKIISLKPDA
                     VQTNIERASFAVGGLDQKRIFEKNLKDIPLICLNMREQDTLVKNVRTYGYILDREEEA
                     EEFIDWYSGYYDLFLSRTASLSEDEKPRCVFEYTPYYCYASGSNLGQVLALAGGKNIL
                     DKKIGPDDPKYGSMLDIEPEFIVKENPEYIFKAVGSWESGYHVDNTSAMAAYQEQVTN
                     RTELANAIAVREDNVHCMSYWILIGAGNNIIGTAYVAKLLHPDLFEDIDPVAIHQEYV
                     DRFCRIDFNVEEKGAFLYPPYDRWPVEA"
     gene            1557..>2044
                     /locus_tag="PLV49_02670"
     CDS             1557..>2044
                     /locus_tag="PLV49_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG97776.1"
                     /translation="MKERITRHWDGRAKAYDENVRQVIYSSRERRIWQKIFSVAIGSA
                     PLRILDVGTGPGIVSNLLSELGHDVTGIDASEGMLKKAQSNSEALHNPMHLIQGDGEV
                     LPFDDGSFDAVVNRYVLWTLPQPERAIAEWQRVLRPGGRLVIVDGTWFDERDRTACRQ
                     LWR"
     assembly_gap    2045..2144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2145..2452
                     /locus_tag="PLV49_02675"
     CDS             <2145..2452
                     /locus_tag="PLV49_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97777.1"
                     /translation="DGTWFDERDRTACRQLWRSLSLILIMLTERRVPCYQEMDKTIKR
                     DLWSCNMKRPDHDIEMLTRLGFRDVQLKDNLNRKLLFTLNHLKHGYGGRQFLVTGVK"
     gene            2485..>3677
                     /locus_tag="PLV49_02680"
     CDS             2485..>3677
                     /locus_tag="PLV49_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG97778.1"
                     /translation="MSFDLLLMNLLLAGNGYASKLRRFSPNARKYLLFVFLTTLNAGI
                     YGVIFNLYILRLGFGEDFLGLILSISATSMGLFSIPAAFVCDRLGRKRTLLLSSVLSA
                     ISLFFLYNTTTPELLVLFSLASGMASALGLVTGSTFLLENSTKEERMYLFSMSSLIYT
                     FSLLSGNMLGGFLPDILADLISAQSGSAISYRLTLYVSLVATVASLLPLAYVAEKNSE
                     ENNGIRGQLHIYRSIFKSKVIRQMTLFYCLYGIGWGTSLPYFNVYFDTVLGASANQIG
                     IFFSVSQVFMILGYFLVPVLTERTGKVRLVSIVQILSIPFLLMFVLTNNLLIAVVGFV
                     MRYMLMNMANPILNSFKLEIVQPEERSMINSIMWMACYTFVGIGNYAGGLMMAAGDSR
                     MPFMVT"
     assembly_gap    3678..3777
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3778..5262
                     /locus_tag="PLV49_02685"
     CDS             <3778..5262
                     /locus_tag="PLV49_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG97779.1"
                     /translation="LVSLFVGISAADDETLKVASPWEPKSLDPIVYQLSVDVSEPLLS
                     VDSEGKLSPMLAQSWDVSEDGRTWTFHLREGVSFHDGTPFNAEAAKLSLERTFRRSAE
                     YSSIKAFPVESVTAPDEYTLVITTSRPFAPLAGYLTKDATEILAASSFDSNDEVVEPI
                     GTGPFKFDSWVPKESMTGVRFDDYWGSKAKVEKVVFLNVPEEKTRESMLRAGEVDIIG
                     TISPALAKKLAEDSSFKVYEQEEMGRVRHLLLNVANPPLDDVLVRQAISMAIDRDLIC
                     DSLLEGMDQTASAPFSSNLYWSNDNLETPSYDPEEAIALLEEAGWSDSDNDGIMDKDG
                     EDLKLSLFTYPSRPELPSIAEALKDQLGQVGIDIEITILDDSAVREQAKGGKVDIYLV
                     SVNTLLNRDPDTWASYFTTESYYYDCMNYAPDDVADLIEKGRETMDQEARKEIYDQLQ
                     ERILKDVPVVYLTYYTGISASSSDIQDYELSLIGRHHLENAYKA"
     gene            5313..>6172
                     /locus_tag="PLV49_02690"
     CDS             5313..>6172
                     /locus_tag="PLV49_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG97780.1"
                     /translation="MYGLILRRIAAAILTISVLTVFVFALLYLFPGDPSERILQTRMN
                     GELPTSRDTVEVLKKEMGLDAPPYQLYLSWARGVLHGDLGYSYVTRRPVITILSESLR
                     ATGELVMISMLIVMFFGLALGFLAAWKEDSWIDDMLSYLAILGISVPGYVTAFVLILI
                     FAIGLHLLPVAGRSGLYSMLMPAASLSFGGVAMLMRMTRYDILEESRQDYVLAAKAKG
                     LGEARVFTGHIFRNSLPPLVTYLGLELGGLFGGAVIVENIFAWPGIGRLLVDSVQSGD
                     VPLVQASVLL"
BASE COUNT         1456 a         1466 c         1611 g         1439 t
ORIGIN      
        1 atgatgcgct tcattgtcct tgcctcacca tttccttttt tccctatctg caaccagagt
       61 agagaatcaa tgcctttcga ctcaaaaaag gggatccaat gggaaaggat ggctcttaaa
      121 gccctaaaaa ttcatcacag tatttattaa ctcgatgtat tatcgtatga aaaagtgact
      181 gctgaattca tgtattatcc cgtgcgaggt ataaaataat ggagccgaac cgcttaattt
      241 ggattttgat ctatgctctc gtgatattat ctcctactaa tgctgctgat ttcgtcctgg
      301 agatctatgg caatgccaat atggatgagt atctggacca ggccgacctg gactatatca
      361 gggatatcgt agcgggcaaa accgagccca ccaagcttgc cgatgccaac ttcgatggcc
      421 aggtgaatga gcaggatgta gagcagctgc aagcgatact ggacggcagg gaggagaagc
      481 tcacattcat cgatatgctg ggcgagccag tgaccgtcaa caagcccatc cgaaggctgg
      541 tgaacatggg ttacaatggg gtggagatga ccagaatcct gggggcagag gatattctgg
      601 tggcctatgg ccaggataga gctgatcatg caatattctt ccccaaattc gctgagctgc
      661 cctttgtggg aagcgatccc aacgactgcg attatgagaa gatcatcagc ctcaagcccg
      721 atgccgtcca gacaaatatc gaacgggcga gctttgccgt gggcggactg gatcagaagc
      781 gcatctttga aaagaattta aaagatattc ccttgatctg ccttaacatg cgcgagcagg
      841 ataccttggt gaagaacgta aggacatacg gatacattct ggatcgagaa gaggaagcag
      901 aggagttcat tgattggtac tctggatact atgatctatt cctgagcaga accgcatccc
      961 tctccgagga tgagaagccg agatgcgtct ttgagtatac cccctactac tgctatgcat
     1021 cgggaagcaa tctgggacag gttctggccc ttgcaggtgg aaagaacatc ctcgataaga
     1081 agatcggccc ggacgatcca aaatacggct caatgctcga tattgagcct gagttcatag
     1141 taaaggagaa tcctgaatat atcttcaagg cagtgggaag ctgggagagc ggctatcatg
     1201 tcgataacac ctctgctatg gccgcctacc aagagcaggt gacaaatcgc actgagctag
     1261 ctaatgctat tgctgtcaga gaggataatg tgcactgcat gagctactgg atccttatcg
     1321 gagcgggaaa caacataatt ggcacagctt acgtggcgaa actcctgcat cccgatctat
     1381 tcgaggatat agatcccgtg gccatacatc aggaatatgt ggaccgtttc tgccgcatag
     1441 acttcaatgt cgaagaaaag ggtgcattcc tctatcctcc gtatgatcgc tggccggtgg
     1501 aggcctgagg atatgatagt cttgccgctt tcgatctacc tttccaggag caggggatga
     1561 aagagaggat aaccagacac tgggacggga gggcaaaggc ctacgatgag aacgtcaggc
     1621 aggtcatata ctcctccagg gagaggcgca tatggcagaa gatcttcagc gtagctatag
     1681 gcagcgcccc cctccggatt cttgacgtgg gcacgggacc gggaattgtg tccaatcttc
     1741 tctccgagct gggtcatgat gtcacaggca ttgatgcctc ggagggaatg ctgaagaagg
     1801 cccagtcgaa ctcggaagct ctgcacaatc caatgcatct aatccaggga gatggcgaag
     1861 ttctgccctt tgatgatggc tccttcgatg ctgtggtcaa caggtatgtt ctctggaccc
     1921 ttccccagcc ggaaagggca attgcggaat ggcagagggt tctgcggccg ggcgggaggc
     1981 tggtgatcgt agatggcacc tggtttgatg aaagagatag gacggcatgc aggcagctgt
     2041 ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntagatg gcacctggtt
     2161 tgatgaaaga gataggacgg catgcaggca gctgtggcgc agtctctcct taatcctgat
     2221 catgctcacc gagagaaggg tgccatgcta ccaggagatg gataaaacga taaaaaggga
     2281 cctgtggtcc tgcaatatga agcggcctga ccatgatata gagatgctca ccagacttgg
     2341 cttcagggat gttcagctta aagataacct caaccgaaag ctgctattta ccctcaacca
     2401 tctgaagcat ggatacggcg gcaggcagtt cctggtcacc ggggttaagt gaagcctcag
     2461 atttttttgc cagaataagc tatcatgtca tttgatcttc ttctgatgaa tttgctcctg
     2521 gcaggcaatg gctatgcctc caagttgagg cgcttcagcc ccaatgcgag aaagtatctg
     2581 ctcttcgtct tcctgaccac tctgaatgcg gggatatatg gggtcatatt caatctctac
     2641 atcctgcgcc tgggcttcgg agaggatttt ctggggctga tcttatccat ctccgccacc
     2701 tccatgggac tcttttccat acctgcagcc tttgtctgtg atcgcctggg cagaaagagg
     2761 acgttgcttc tctcttcagt tctcagcgct atatcgctct tctttctcta taataccaca
     2821 acaccggagc tattggtgct attcagcctg gcatccggga tggcatccgc cctgggcctg
     2881 gtgaccggat cgaccttcct cctggaaaac tcgaccaaag aagagaggat gtatctcttc
     2941 tccatgtcct cgctcatcta caccttttcc ctcctatcag gaaacatgtt gggcggattt
     3001 ctgcccgata tcctggcaga tctcatctct gcccagtctg gcagcgccat atcttatcgg
     3061 ctaactctct atgtgtcatt ggttgccact gtggcatcgc tgctgcccct ggcatatgtc
     3121 gctgagaaga attcggaaga gaataatggc atcagaggcc agcttcacat ctatcgatcc
     3181 atattcaagt ccaaggtcat cagacaaatg accctatttt attgccttta tgggatagga
     3241 tggggcacat cccttcccta tttcaatgta tacttcgata cggttctcgg tgccagtgcc
     3301 aaccagatcg gcatcttctt ctccgtctcg caggtcttca tgattctcgg atattttctc
     3361 gtccccgttc tcaccgaaag gaccgggaag gtccggctgg tctccattgt gcagatctta
     3421 tcgataccat tcctgctgat gtttgtcctt accaataacc tgctcattgc cgtggttgga
     3481 tttgtgatga gatatatgct catgaatatg gcaaatccca tcctgaatag ctttaagctg
     3541 gagatcgttc agcctgagga gaggtcgatg ataaacagca taatgtggat ggcctgctac
     3601 acctttgtag gaattggaaa ctatgccggt ggccttatga tggctgcagg cgatagccgc
     3661 atgcctttta tggtcacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctg
     3781 gtcagcctct tcgtgggcat ctctgcagca gatgatgaaa cgctgaaggt ggcatcgccc
     3841 tgggagccaa aatcgcttga tcccattgta tatcaattga gcgtggacgt ttctgagccg
     3901 cttctgagtg tggactcaga gggaaaacta tcgccaatgc ttgcccagtc atgggatgta
     3961 tccgaggacg gcagaacatg gacatttcac ttaagagaag gagtcagctt ccatgatgga
     4021 acgcctttta atgcagaagc tgcgaagctg agcttggaga ggaccttcag aagatcggca
     4081 gaatattctt ccatcaaggc atttccagtg gagtcagtca cagcgcctga tgagtacacc
     4141 ctagtgataa ccaccagcag gccatttgct cccctggcag gatacctgac caaggatgca
     4201 actgagatac tggcggcatc atccttcgac agcaatgatg aggtcgtaga gcccataggc
     4261 accggcccct tcaagttcga ctcctgggtg ccaaaggaga gcatgacagg tgttcgattt
     4321 gatgactatt ggggctccaa agcaaaggtg gagaaggtgg tatttctgaa cgtccctgaa
     4381 gagaagacca gggagagcat gctgagagca ggcgaggtgg acataattgg cactatctct
     4441 cctgctctgg ccaagaagct ggccgaggac tccagcttca aagtctacga gcaagaggag
     4501 atgggcagag tacgccatct actgctgaat gtagctaatc cgcccctgga tgatgttctg
     4561 gtgcgtcaag cgataagcat ggcaatagac cgcgatctga tctgcgattc cctcctggag
     4621 ggtatggatc agacggcatc agctcctttc tcatccaatc tctactggtc aaacgataac
     4681 ctcgaaaccc catcatatga cccagaggag gcaattgcac tcctggaaga ggccggctgg
     4741 tcggacagcg ataacgatgg catcatggat aaggatgggg aggatctcaa attatctctg
     4801 ttcacctatc cgagcaggcc ggagcttcca agcattgcag aggcgctgaa agatcagctg
     4861 gggcaggtgg gaatcgatat agagatcacc attctcgatg actctgcagt gagagaacag
     4921 gccaaaggag ggaaggtgga catctatctg gtctccgtca atactctcct taatagagac
     4981 ccggacacct gggccagcta ttttactacg gaatcgtatt actacgactg catgaactat
     5041 gccccggatg atgtggccga cctcatcgaa aaaggaaggg agaccatgga ccaggaggcg
     5101 agaaaggaga tctacgacca gcttcaagag aggatactga aggatgttcc tgtggtatat
     5161 ctgacctact acacgggcat ttccgcctca agctccgata tacaggacta tgagctgagc
     5221 ctgatcggca ggcatcacct ggagaatgcc tacaaagcct gaatgaaaag agggagagcg
     5281 agaggaacaa gagcgaggga cgggagcagg acatgtacgg gctcatcctg aggcggattg
     5341 ccgctgccat tctgaccata tccgttctca ccgtatttgt cttcgccctc ctctatctct
     5401 tcccaggaga tccttcggag cgaatactgc agaccaggat gaacggtgag cttcccactt
     5461 cccgtgatac agtggaggtc ctgaaaaagg agatggggct ggatgcacct ccatatcagt
     5521 tatacctgag ctgggcccgc ggggtgttgc atggcgatct gggctactct tatgtcacca
     5581 gaaggccggt cataaccatt ctcagtgagt ccctccgggc gaccggcgag ctggtgatga
     5641 tctccatgct gatagtcatg ttctttgggc ttgctctggg ctttctagcc gcctggaagg
     5701 aggattcgtg gatcgatgat atgctcagct atcttgccat tctgggcatc tccgtgcccg
     5761 gatatgtcac agccttcgtc ttaatcctca tatttgccat aggcctgcat ctgcttcccg
     5821 tggcgggacg aagcgggctg tattccatgc tcatgcctgc agcatcgctc tcctttggag
     5881 gggtagcgat gcttatgcgc atgacccgat acgatattct ggaggagtca aggcaagact
     5941 acgtcctggc agcgaaagcg aagggcctgg gcgaagcacg ggtgttcaca gggcatatat
     6001 tcaggaattc cctccccccg ttggtcactt acctcgggct ggagttggga ggcctattcg
     6061 gcggggcggt gattgtggag aacatttttg cctggccggg aatcggccgg ctcctggtgg
     6121 actcggtcca gtcaggagac gtcccattgg ttcaggccag cgtcctctta at
//
LOCUS       DAOSGP010000054         6131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11280_length_6131_cov_16.172482, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000054 DAOSGP010000000
VERSION     DAOSGP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11280_length_6131_cov_16.172482"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1336
                     /locus_tag="PLV49_02695"
     CDS             <1..>1336
                     /locus_tag="PLV49_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG97781.1"
                     /translation="VRMMIIHQFFVPGIAHSSYMVAGNNVCAVIDPERNIRPYLDSAK
                     RMGLKITHILETHLHADFISGHLDLEEATGARIYAPASANCAFPHEPLKEGDRIKLED
                     IIFSVIETPGHTPEHISFIAADTSRADDPVSLFCGDTLFVGDVGRPDLFPVRAEELAS
                     SLYDSLHEKILKLPDHCEVYPAHSMGSLCGRAISAKRSSTIGFERRYNYALQISDRQE
                     FIHSLTSDMPAAPDHFARCSEINRVGPSLMRSLKIPKPLDPKAFSNSIKSEKASILDV
                     RSYPAFSGLHIPGSWHIDLAGSFATQAGWVIPPKKDIFLVVDGIEQVDEAAMQLRRVG
                     LDSVEAFLERGMLAWGVNAMPVDTVPIITPNKANDLVASEKAVIIDVRSAEEKTDDCV
                     IGSIQIPWHDLRTRHKELSRDRKYIVMCRGGQRASIAASILKMNGFNNVFNMA"
     assembly_gap    1337..1436
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1437..1695
                     /locus_tag="PLV49_02700"
     CDS             <1437..1695
                     /locus_tag="PLV49_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOG97782.1"
                     /translation="AILGYCPGTAAAAFGSGAIDALVGMVGIIIGAGMFARLYPVLNR
                     SVLKWGPLPEGTIPELIGIRPTIVIAAVAVLIVAVLRWLSF"
     gene            1989..4847
                     /locus_tag="PLV49_02705"
     CDS             1989..4847
                     /locus_tag="PLV49_02705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG97783.1"
                     /translation="MREDYISQKPAGLIECAVDVLALAPDLAAKFLLDLPIHPLKGVV
                     YADSMRFWSRLALYSLELVAKEQFMPAVRERYSLWKAVMDETDLERLDRFSKAMPPSC
                     QALRSFPTDKSSPKDLARSFVDGMADSLVRGSLEQLTLLPPKRGRRPRVIPLPQQFLL
                     SLTSKDPTLEASTEEVAAFAKKMNSWISELEPKAVSVPFRTCFRLQAPAEEQTWRLSF
                     FLQAKDDRSLMVPAGEVWRTKSDALTLLKKSIKNPQEQLLADLGRAARLFPQLEKSLE
                     TARPAELEMETTEAYSFLRQSAPLLEQNGFGVMLPAWWQRPGTRLGVKLQLRDAPRKE
                     GNAGSAFFGLNSVVEYDWQLSLGDYVISREEFAQLSHLKIPLIQIRGEWMELRPQDIE
                     AAIDFFHKNRNHEMTLADALHLGLNPAGHPLPEAKTTLPVHGFEAEGKIKEMLSALSD
                     PSGHMEQLSSPLDFQGTLRPYQLRGVSWLMYLNRLGLGACLADDMGLGKTIELIAFLL
                     HERETMKGPGPTLLICPMSVAGNWEREIERFAPGLKVLMHHGLGRLSGQDFEKAAEKA
                     DVVITTYSLAQRDEEELNAVSWGHIVLDEAQGIKNQSARQTQSIKRLKATQKIALTGT
                     PVENRLSELWSIMDFLNPGYLGSFESFRKDFVLPIERYNNKAHSEALRSLIQPFVLRR
                     LKTDSTVIKDLPEKTEMKVGYNLTPEQASLYAAVVEDMLSRIESSEGMKRRGQILAAL
                     TKLKQICNHPALFLQDGSPLEGRSGKLSRLEEMLDEAISVGDRALIFTQFAGMGAMLR
                     HQLQEKLGVEVLFLHGKTSRKQREEMIQRFQQGSSPIFILSLKAGGFGLNLTAANHVF
                     HFDRWWNPAVENQATDRAFRIGQKKNVFVHKFVCAGTLEERIDQMIEQKKALAESVIG
                     TGEAWLTELSDDSLRELLVLRHETVSNGSDSSDRSDRK"
     gene            4844..5737
                     /locus_tag="PLV49_02710"
     CDS             4844..5737
                     /locus_tag="PLV49_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOG97784.1"
                     /translation="MSPYWSYYEPAKPKKVENGLKAKSKRGCIGETWWSKRWVKTLES
                     FNMGARLTRGKSYARKGQVISIDVNPGIVRARVQGTGSEPYDVVIKLKPLSDAEWDKA
                     ASTMASKAVFAARLLGGEMPQNIEEAFSESGLSLFPSKGRDLKTDCSCPDWSNPCKHI
                     AAVYYLLAEQFDCDPFLIFKLRGRSKEQIMEAMRKMRSAEAVEEQDGVLRSSQDAREQ
                     FKPLEECLDCYWQGGGELDLLEINPRAPDVKGAVLERLGPSPFYIAGVNLAALLPKAY
                     EKAGKAALLRATGETESIEMQ"
BASE COUNT         1445 a         1583 c         1661 g         1342 t
ORIGIN      
        1 aggtgagaat gatgatcatc catcaatttt tcgtccccgg catcgcccat agctcctaca
       61 tggtagccgg gaataacgtc tgtgcagtca tcgacccgga acgcaacatc cgcccttatc
      121 tggattctgc caagaggatg ggtctcaaga taactcatat cctggagacg cacctgcatg
      181 ctgacttcat ctccggccac ctcgatctgg aagaagcgac tggagccagg atttatgctc
      241 cagcatctgc gaactgcgct tttccccatg agccattaaa agaaggggac aggataaagc
      301 tggaggatat catattctcg gtgatcgaga ccccaggcca caccccagag cacatctctt
      361 tcatcgctgc tgacactagc agggcagatg accctgtctc cctcttttgc ggtgacaccc
      421 tctttgtagg agacgttggc aggccggatc tctttccggt cagagcagag gagcttgcat
      481 catccctcta tgatagcctg cacgagaaga tcctgaagct tccggaccac tgcgaggtct
      541 atcctgctca tagcatggga tcgctctgcg gacgggcgat aagcgctaag aggagcagca
      601 ccatcggctt tgagagaaga tacaactatg ccctgcagat atccgatcgc caggagttca
      661 tacattccct cacctcggat atgcctgctg ccccggacca cttcgctcgc tgctcagaga
      721 tcaacagggt ggggcccagt ctcatgagga gcctcaaaat ccccaaacct ttggatccga
      781 aggccttttc caatagcata aaatccgaaa aggcgtcaat cttggacgta cgaagctatc
      841 cggcattctc cggcctgcat atacctggat cctggcatat cgatcttgcc ggcagcttcg
      901 ccacccaggc aggctgggtc atacccccga aaaaggacat cttcctggtg gttgatggga
      961 ttgaacaggt ggacgaggct gctatgcagc tgcgccgggt aggtctggat agcgtggaag
     1021 cgttcctcga aagaggcatg ctggcctggg gagtgaacgc tatgccagta gatactgttc
     1081 caataataac ccccaataag gcaaatgatc tggttgcatc ggaaaaggcg gttatcatcg
     1141 atgtccggtc agcggaggag aagactgacg attgcgtcat cggatcgatt cagatcccct
     1201 ggcacgacct cagaaccaga cataaagagc tatccaggga taggaagtat attgtgatgt
     1261 gcagaggagg acagagggcg agcattgcgg catccatcct gaagatgaac ggatttaata
     1321 acgtctttaa tatggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgct
     1441 attctgggct actgtccagg aaccgctgct gctgcatttg ggtcaggagc gattgatgcc
     1501 ctggtgggga tggttggcat cattattgga gcggggatgt tcgcccgcct ctatcctgtc
     1561 ctgaaccggt cggtgctaaa atggggcccc cttccagagg gcacaatacc agagcttata
     1621 ggcatcaggc cgacaatcgt cattgctgcc gtggcagtct tgatagtcgc cgtgctgcgc
     1681 tggctaagct tttaaataac aatataacct aagcccgatt gggccaaagg acttaaagca
     1741 tgtacgtctt gcactctctc tgggataccg aggcatttta tatctgggct gaatcctcag
     1801 ctctacctct ggtcaatcag ggctattcag ttagcagcga agggattcca gctcatccat
     1861 tctccttgcc gggaaaggag ctaaaaacgc aaatttccag ggcatttcat ctgaatggcg
     1921 ccaagactcg agcactgatt ctcaggattc cttccggaaa aaatggacct cttccctcgc
     1981 cctggctatt gagagaggat tatatctcac aaaagcctgc ggggctgatc gaatgcgctg
     2041 ttgatgttct agcccttgcg cctgacctgg cagccaaatt tcttctggat cttcccatac
     2101 atccgctcaa gggagttgtt tatgctgatt caatgcggtt ttggtccagg ctcgccctct
     2161 attccctgga gctggtggca aaagagcagt ttatgcctgc ggtcagagaa agatactctc
     2221 tatggaaggc ggtgatggat gagacagatc tggaacggct tgatcgcttc tcaaaagcca
     2281 tgccaccaag ctgccaggct ctcagatcgt tccccacaga taaatcctct ccaaaagatc
     2341 tggcgagaag cttcgtggat ggaatggctg actctctggt gcgcggcagc ctggaacagc
     2401 ttacactcct cccacccaag agagggcgca gaccgcgagt gattcctctg ccccagcagt
     2461 tcttgctgtc tcttacctca aaggacccaa cgcttgaagc ctcgactgag gaggtcgccg
     2521 ccttcgccaa gaagatgaac tcctggatca gtgaactcga gccgaaagcg gtgtctgtac
     2581 catttcgcac ctgcttccgg ctgcaagccc cggcggaaga gcagacctgg agattgagct
     2641 tcttcctcca ggcaaaagac gaccgcagcc tgatggtccc ggcgggagag gtatggagga
     2701 caaagtcgga tgctctcacc ctcttgaaaa agagcataaa gaatccgcaa gagcagcttc
     2761 tcgctgacct gggcagagca gcgcgactct ttccccagct ggaaaagagc ctggagactg
     2821 ccaggcctgc agaactggag atggaaacca cagaagctta ttcattcctg cgccaatcgg
     2881 ctcccctttt agagcagaat ggcttcggag taatgctgcc cgcctggtgg cagcgacccg
     2941 gtacacgcct gggggtgaag ctgcagctga gggacgctcc caggaaagag ggcaatgctg
     3001 gttctgcttt cttcggcctg aactcggttg tggaatacga ctggcagcta tctttaggcg
     3061 actatgttat ctctcgagag gagttcgcac agctctctca cctcaagatc cctctcattc
     3121 aaataagggg agagtggatg gagctgaggc ctcaggatat cgaggcggcc attgattttt
     3181 tccataagaa caggaaccac gagatgaccc tggctgatgc tctgcatctg ggcttgaatc
     3241 cagccggcca tcctttgcct gaagcgaaaa ccacccttcc cgtgcatggc tttgaggccg
     3301 agggcaagat caaagagatg ctttccgctc tttctgaccc cagcggccat atggagcaac
     3361 tctcttcccc cctagatttc cagggcactc tgcggcccta tcagctacgg ggcgtatcct
     3421 ggctcatgta cctgaaccgg ctcggcctgg gagcctgtct ggcggatgat atgggtctgg
     3481 gaaaaacgat agagttgatt gcatttcttc tgcatgagag agagactatg aagggccccg
     3541 gccccactct tctcatctgt cctatgtctg ttgccggcaa ctgggagcgg gagatcgagc
     3601 gctttgcccc cgggctgaag gtcctcatgc atcacggcct gggacgcctc tccggccagg
     3661 acttcgagaa ggcggcggaa aaggcagatg tggttatcac cacctactct cttgcccaga
     3721 gggatgaaga agagcttaat gccgtatcct ggggtcacat agtcctggac gaggcgcagg
     3781 gcatcaagaa ccagtctgcc cggcagaccc agtctattaa gcgcctgaaa gccacccaaa
     3841 agatcgctct gacaggaacg cccgtggaga acaggctctc tgagctatgg tcgatcatgg
     3901 atttcctcaa tcccggctat ctgggctcat tcgaatcctt ccgcaaggat ttcgtgctgc
     3961 ccatagaaag atacaacaat aaggctcatt ccgaggccct gcgatctctc atccagccct
     4021 ttgtcctgcg ccggctaaaa accgactcca cagtgataaa ggacctccca gaaaagacgg
     4081 agatgaaggt gggctataat ttgacgccgg agcaggcgag cctttatgct gctgtggtgg
     4141 aggatatgtt atccaggatc gagtcttctg aaggcatgaa gcgcagaggc cagattctgg
     4201 ctgcactgac caagctcaaa cagatctgca accatccggc gctcttcctg caggacggaa
     4261 gcccgctgga gggccgctct ggaaagctgt cccgtctaga ggagatgctc gacgaggcca
     4321 tttctgttgg tgacagggcc ttgatattca ctcagttcgc cggcatgggg gcgatgctca
     4381 gacatcagct gcaggaaaag ctcggggttg aagtcctctt cctgcacggc aagaccagcc
     4441 ggaagcagag agaggagatg atccagaggt tccagcaggg aagctctcca atattcatac
     4501 tgtcattaaa ggcaggaggc ttcggcctca atctgactgc tgccaaccac gtattccact
     4561 ttgatcgctg gtggaatcct gcggtggaga atcaggctac tgaccgcgcc ttccgcatcg
     4621 gccagaagaa gaacgtcttt gtgcataagt tcgtctgtgc tggaacgctt gaggagagaa
     4681 tagaccagat gatagaacag aagaaagcgc tggcagaatc ggtgataggg accggtgaag
     4741 cctggcttac ggagctgtct gatgatagct tgagagagct gctcgttctg aggcatgaga
     4801 cagtgagcaa tgggagcgat agcagcgatc ggagcgatag aaaatgagcc cctactggag
     4861 ctactatgaa ccagccaagc ccaagaaggt cgagaacggg ctaaaagcca agagcaaacg
     4921 aggatgcata ggagaaacat ggtggtccaa gcgctgggtc aagacccttg aatccttcaa
     4981 tatgggtgcc aggctcactc gaggaaaaag ctatgctcga aaagggcagg ttatatccat
     5041 agatgtcaat cccggcatag tgagggccag ggtgcaggga accggatcgg agccttatga
     5101 tgtggtgata aagctgaagc cactgtcgga tgctgagtgg gataaggctg cctcgactat
     5161 ggcctcaaaa gcagtatttg cagcccggct gctgggcggg gagatgccgc agaacatcga
     5221 agaggccttc tccgagtcag gtctatccct ctttcccagc aagggcaggg acttgaagac
     5281 agattgctcc tgcccggact ggtctaatcc ctgcaagcat atagctgcgg tctactatct
     5341 tctggcagag cagtttgact gcgatccttt tctgatcttc aagcttcgag ggcggagcaa
     5401 agagcagatc atggaagcga tgcgcaagat gcgttccgct gaggctgttg aggagcaaga
     5461 cggtgttttg agatcaagcc aggacgcacg ggagcaattc aagcccttgg aggagtgttt
     5521 ggattgctac tggcagggag gtggcgagct tgatctgctg gagatcaatc caagagcccc
     5581 tgatgtaaag ggggcagttt tggagagact ggggccgagc cccttttaca tagcaggtgt
     5641 caatttggca gcattgctcc ccaaggccta tgaaaaagca ggcaaagcag cacttctcag
     5701 ggcaactggc gagactgaaa gcatagagat gcagtagtaa agatggcact ggctatgcca
     5761 cgcttcacga gctggatgct ggaaggcaag agactgcaga aagctattgc cgaaaccttg
     5821 atgagtgcat tattgactga aagagcttgc tcatccagtt aacaaatcct ttaaatggac
     5881 ttgtcttcat atacctggat gcgtccaggc accttccata gctatcgtag acctcgaatt
     5941 tcccatcttc actatggatg atgcactcag ccttctcttt cctgcatatc tctcgtgcca
     6001 gcctgaccgc ctcggccttg gtctgagcgt gaccacaagg cctccatgca tggggcttct
     6061 tgatatccca tccgtttcgg gaattcttta ccacatgtcg ctcattccga gccatatttt
     6121 tctattagtg t
//
LOCUS       DAOSGP010000055         6112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11341_length_6112_cov_19.314017, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000055 DAOSGP010000000
VERSION     DAOSGP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11341_length_6112_cov_19.314017"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PLV49_02715"
     CDS             complement(<1..752)
                     /locus_tag="PLV49_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG97785.1"
                     /translation="MAEEKATAKTSVLLEETRIFEAPKDLAENSNVMKWMKEKGFKTE
                     REMRLWCSANYIQFWGEMAKTYADWFEPWSSTLEWKPPYAKWFVGGKCNVTHNCVDRH
                     AQGAKKDKVAYIFVPEPTDQPTQKITYAQLEKEVNKFANGLKSLGVVKGDRVMLYMPM
                     IPQLPIAMLAIAKIGAIHCIVFSGFSSGGLNSRILDAEAKVVVTVDGFYRRGKPLALK
                     PNVDEATSNTPSVQKVVVYKRTGVAVPMKEGK"
     gene            complement(1118..3136)
                     /gene="acs"
                     /locus_tag="PLV49_02720"
     CDS             complement(1118..3136)
                     /gene="acs"
                     /locus_tag="PLV49_02720"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOG97786.1"
                     /translation="MLELAGKEDKKLKTTVFQDETRIFNPPKDLVENSNVMQWMKKKG
                     FKTENEMRAWCSSDEHYLEFWDEMAKTYVDWHKPYTKVMDDSEMPYFHWFTGGEINIT
                     YNAVDRHAKGAKKDKVAYIWIPEPTDQPVQKITYGDLYKEVNKFANGLKSLGLKKGDR
                     VSIYMPMIPQLPIAMLACAKLGVIHSVVFSGFSSKGLMDRAADCGSRAIITVDGFYRR
                     GKPVPLKPNADEAASGAPSVEKIIVYKRAGVDVAMKEGRDIWWHDLVKGQSEECEPVW
                     VDPEHRLYILYTSGTTGKPKGIEHATGGNAVGPAQTLHWVFDLKDDDVWWCTADIGWV
                     TGHSYIVYAPLILGMTSLMYEGAADYPDFGRWWKNIQDHKVTVLYTAPTAVRMFMKQG
                     PEWPAKYDLSSLRLLGSVGEPINPEAWMWYREHIGRGELQIMDTWWQTETGTFLNSPL
                     PITPLKPGSCTFPLPGYDISILDEDGNEVPLGSGGNIVALKPYPSMLRAFWGDKERFM
                     KEYWQFYWDVPGRRGVYLAGDKAQRDKDGYFFIQGRIDDVLSVAGHRIANAEVESALV
                     AHPKIAEAAVVGKPDEVKGESIVAFVILRVGNEPSPELAKDAIAFVRKTLGPVAAPTE
                     VHFVNDLPKTRSGKIMRRVVKARALGNPVGDISTLMNPEAVDGIPKIV"
     gene            complement(3513..4826)
                     /locus_tag="PLV49_02725"
     CDS             complement(3513..4826)
                     /locus_tag="PLV49_02725"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HOG97787.1"
                     /translation="MRFYIETFGCTSNFGNSQDLAEALRGMGHIPSGLKEADMVIVNT
                     CAVTERTERKILRRLRQLEGEKLVVAGCLAAAIPQSIQTLCCRGRLGPLSQGDAARIA
                     KLFNGWLMPSQHMQSGQLQSQNFQSEEMLRERHIAVHLRESSPGGESCGIVNVADGCN
                     GSCSYCIVSKARGRLKSRPVEDVVLAVKRLAQLGTAEIQISAQDTAAFGSDIGSDLAG
                     LLETLTEIPGDFMLRVGMMNPDSARLIQDRLIEAFQSPKIYRFLHIPVQSGSNEILQR
                     MGRGYTSDEFFELVSAFRFAYPDISIITDIIVGFPGETDKDFEESMSLIKRLQPDKVN
                     ITRFSPRPGTSAARLYDMPDRIKKDRSREMTRIWLEIAERRNSRYLGKVLHALVTECG
                     RGKTMKARSANYAGIVIPGALDLGRWCQIKIMETTPYYLSGILQL"
     gene            complement(4823..5863)
                     /locus_tag="PLV49_02730"
     CDS             complement(4823..5863)
                     /locus_tag="PLV49_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG97788.1"
                     /translation="MDYDPDRWCSEHKGILILALAAVLMTACFAFPFLDGIILGSVFA
                     YVGRPIRDRFGKRKKLGSLVAAICVVVPIFLMLGLGSLEVAGQIIALAKNQEAFRYWL
                     GYIMQQTATDLPPWARESLISGLENAFGLIASLAASIPILQIGRMASLGIINFLISFP
                     ICYFILLDGEGFVGSLISLLPDGEMRVLERYIDRIDRILSGIFIGTVYTSIVGSLIAA
                     VIFFLFGIPRPIALASIVFIAGMVPVLTSWAVLVPLAVYRYFTVGLEGALFFLVLSSA
                     LIYLPSELFIRPYIISTRSSLHPLLVMLSFFGGALVAGIGGFFLAPAVIGAISGIYQV
                     RREETASSEALE"
     gene            complement(5906..>6112)
                     /locus_tag="PLV49_02735"
     CDS             complement(5906..>6112)
                     /locus_tag="PLV49_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97789.1"
                     /translation="HRDAQKRSRLEKRRTIVAGEVTRSIYFLALENARSSVIISLCNS
                     GASLLLNLHSLETLIKRHPYLSSE"
BASE COUNT         1409 a         1699 c         1532 g         1472 t
ORIGIN      
        1 tctttgccct ctttcatggg gacggcaacg cctgtcctct tgtagaccac taccttctgc
       61 actgatggag tattagaagt agcctcgtcg acatttggct tgagggccag tggcttgccg
      121 cgccggtaga aaccatccac agtcacaaca acttttgcct cagcatccag aatcctgctg
      181 ttcaggccgc cggaagaaaa tccggagaaa acaatgcaat gaatggcgcc aatcttggct
      241 atggcgagca tggcaatggg cagttgaggg atcatgggca tgtataacat gactctgtct
      301 cccttgacca cgcccaggct cttcaggccg ttggcgaatt tattgacctc cttttcaagc
      361 tgtgcataag tgatcttttg agtgggctga tcggtgggct caggcacgaa tatataggcc
      421 accttatctt tcttggcccc ctgggcatgc ctatccacgc agttgtgggt cacattgcac
      481 ttgccgccca cgaaccattt ggcatatggt ggcttccatt ctagggttga ggaccagggc
      541 tcaaaccaat ctgcgtaggt cttggccatc tctccccaga actggatgta gttggcagag
      601 caccaaaggc gcatctcgcg ctctgtcttg aagcctttct ctttcatcca cttcattacg
      661 ttggagttct ctgccagatc cttgggtgcc tcgaatatcc ttgtctcttc cagtaaaact
      721 gatgtctttg cagttgcttt ttcctcagcc aaatttacag cctcctttaa aatccccacg
      781 tcttcccaac tctccgcctg atctgtcctg atgccaggca gccagcccaa cgcagccatc
      841 tggcattctt ttattatcgc aatagggcca aaatcccagc cagcaggagc cattgggaat
      901 attgtagcga tcgataatta acctgttttt tcatttataa atctatcgag gtgacttaac
      961 attatgattg ataattatca ttatttaaca tgattgtaat cattcagctt ttgaaaattg
     1021 ccatattgca aggatgagaa cgccatagca atcaatcaag acttgacgaa aaaagaagaa
     1081 tagcttcgca atctgataaa aaaaaatgag atccccatca gacgatcttg gggatcccat
     1141 ccactgcttc cggattcatg agggtcgata tgtccccaac cgggtttcca agggccctcg
     1201 ccttgacgac acggcgcatt atcttgccgc tcctcgtctt cgggaggtcg ttgacgaagt
     1261 ggacctccgt gggcgcagcc accggcccaa gggtcttcct gacgaaagcg atcgcatcct
     1321 ttgccagctc aggagacggt tcatttccga ccctcaggat gacgaaggcg acgatcgact
     1381 cgcccttaac ctcgtcgggc tttccaacta ctgccgcctc ggcaatcttg gggtgggcca
     1441 ccaaggcaga ctcgacctcg gcgttggcta tcctgtggcc tgcgacactg aggacgtcat
     1501 cgattctgcc ctggatgaag aagtagccgt ccttatccct ctgcgcctta tctccggcga
     1561 gatagacgcc gcggcggccg ggaacatccc agtagaactg ccagtactcc ttcatgaacc
     1621 tctctttgtc gccccagaac gccctgagca tcgacggata gggcttcaag gccactatat
     1681 tgcctccgga tcccagagga acctcattcc catcctcatc caggatggat atgtcatatc
     1741 cggggagcgg gaatgtgcac gatccgggct tcagtggcgt gattggcagc ggagagttga
     1801 ggaaggttcc ggtctcagtc tgccaccagg tgtccatgat ctggagctcg cctcgtccaa
     1861 tgtgctcacg ataccacatc caggcctccg gattgatcgg ctcgcccaca gatccgagca
     1921 gcctcaggct tgagaggtcg tatttagcag gccattctgg gccctgcttc atgaacatcc
     1981 ttaccgccgt gggggcagtg taaagaacgg tgaccttgtg gtcctggatg ttcttccacc
     2041 acctgccgaa gtctggatag tctgcagctc cctcgtacat aaggctggtc atgcccagaa
     2101 tgagcggagc gtagacgata taagagtgac cagtgaccca tccgatatcg gcggtacacc
     2161 accagacatc gtcgtccttc aggtcgaaga cccagtgaag cgtctgagcc ggtccgacgg
     2221 cgtttccgcc cgttgcgtgc tcgataccct tgggcttgcc ggttgtgccg gaggtgtaga
     2281 ggatatacag cctgtgctcc gggtcaaccc ataccggctc acactcttcg gactggccct
     2341 tgaccagatc atgccaccag atgtccctgc cctctttcat ggcaacatca acgcccgccc
     2401 gcttgtagac gataatcttc tcgactgacg gagcgccgct agctgcctcg tcggcgttcg
     2461 gcttcagggg caccggcttt ccgcgcctgt agaatccatc cacggtgatg atggctctgg
     2521 aaccgcagtc agcagccctg tccatcaggc ctttggagct gaatccggag aaaaccacgc
     2581 tgtggatgac gccgagcttg gcgcaggcga gcatggcgat gggaagctgg ggtatcatgg
     2641 gcatgtagat gctgacccga tcccccttct tcaagccgag gctcttgagg ccgtttgcga
     2701 acttattgac ctccttatag aggtcgccgt aggtgatctt ctggactggc tggtctgtcg
     2761 gctctggtat ccatatgtat gcgactttgt ccttctttgc gcccttggcg tgcctgtcca
     2821 cggcgttgta tgtgatgttg atctcacctc cagtgaacca gtggaagtag ggcatttccg
     2881 agtcgtccat caccttggta tagggcttgt gccaatcgac gtacgtcttt gccatctcgt
     2941 cccaaaactc gaggtaatgc tcatcagagg agcaccaagc acgcatctca ttctctgtct
     3001 tgaacccttt cttcttcatc cactgcatta cgttggagtt ctccaccaga tccttcggcg
     3061 gattaaagat tctggtctcg tcctgaaata ctgtcgtctt caacttcttg tcttctttgc
     3121 cagccaattc caacacctcc taaatcaaag cctaatattt cgcgcacttt tctcctatcc
     3181 cagggaaaac ttgacgcgaa gaacatgccc gctgtccact ctaattcgtc acagagaaca
     3241 gcagttttgc acatttccga tttgtctttc agataaataa ggtttttgct tgttaataat
     3301 tataaataat aatgattgta gatcatatgg cttgaagaga ctgtcgaatc tagaagagtc
     3361 tcgaagcaga cgtgcccggc cgccggaaga atactgttaa tgccagctca tttatggtca
     3421 cagatatgaa gaaattaatg caaaaatagc atcgcgagca acaactatag ctcttttccg
     3481 cagcaaaacc taaataaaaa gctgactatg aatcataatt ggagaattcc acttaaataa
     3541 tatggagtgg tttccatgat ctttatctgg caccatcttc ccagatccag cgcgccgggg
     3601 atcacaatgc cagcataatt ggcagatcgg gccttcatgg tcttgcctct cccacactcc
     3661 gtcacaagag catgcaatac tttccccaga taacggctat tcctcctctc agcgatctct
     3721 agccatattc gggtcatctc tctcgagcgg tctttcttaa tcctgtccgg catgtcgtag
     3781 agcctggcgg ctgatgttcc aggccgaggg gagaatctgg tgatattcac cttatccggc
     3841 tgcaatctct taatcagact catgctctcc tcaaaatcct tatccgtctc gccgggaaag
     3901 ccaacgataa tatcagttat tatggagatg tctggatagg caaatcgaaa ggcgctcaca
     3961 agctcgaaga actcatctga ggtgtatccg cgtcccatcc tctgcaggat ttcattcgag
     4021 ccggactgaa cggggatatg caggaaacgg tagatctttg ggctctgaaa tgcctcaatc
     4081 agcctatcct ggatgagccg agcgctatcg ggattcatca tgccaaccct gagcataaaa
     4141 tcccctggaa tctcagtcag cgtctccagc agcccggcca gatcagatcc aatatcagat
     4201 ccaaatgcgg cagtgtcctg agcagatatc tggatctcgg ctgtgccaag ctgtgccagc
     4261 cttttcacgg caaggaccac atcctcaacc gggcggctct tcagcctgcc cctggccttt
     4321 gagactatac aatagctgca tgagccgttg cagccgtccg ccacattgac aatgccgcag
     4381 ctctctcctc cgggagatga ttcgcgaaga tgaacagcaa tatgcctctc gcgcagcatt
     4441 tcttcagatt gaaaattctg agattgtaat tgcccggatt gcatatgctg cgaaggcatg
     4501 agccaaccat taaagagttt ggcgatcctg gcggcatctc cttgactcaa aggtcccaat
     4561 cgcccccggc agcagagcgt ttgaatcgac tggggtattg ccgccgccag gcagccggcg
     4621 acgaccagct tctcaccctc cagctgccgc agccgacgga ggatctttct ctccgtcctt
     4681 tcggtgaccg cacaggtgtt cactatcacc atgtcagcct ccttcaggcc ggaagggata
     4741 tggcccatcc ccctcagggc ctctgccaga tcctgagaat tcccgaagtt cgatgtgcag
     4801 ccgaaggtct cgatataaaa cctcattcca aagcctcact tgaggccgtc tcctcccttc
     4861 tcacctgata aatcccactg atcgccccta tgaccgccgg ggcgaggaaa aagccgccga
     4921 tccctgccac cagagcccct ccaaaaaagg agagcattac cagcaatggg tgcaatgagg
     4981 atctggtgga gatgatatag ggccttatga acagctctga ggggagatag atgagggcgg
     5041 agctgaggac caggaagaat agagcaccct ccaggcccac ggtgaagtag cggtagacgg
     5101 ccagggggac caggaccgcc caagaggtga ggaccgggac catgccggcg ataaagacga
     5161 tgctcgccaa ggctataggc cggggtatgc cgaagaggaa gaatatcacc gctgcgatca
     5221 ggcttcccac gattgacgta tagacggtgc cgatgaatat gccgctcaga attcggtcga
     5281 ttctatcgat gtaccgctca agaaccctca tctctccgtc tggcaggagg gatatcaacg
     5341 atcccacaaa gccctcgcca tccaggagga tgaagtagca gatggggaag gagatgagaa
     5401 agttgattat ccccaaagag gccatcctgc cgatttggag gatgggaata gatgctgcca
     5461 gagaggcgat caggccgaag gcgttctcca gaccggagat caggctctcc ctggcccagg
     5521 gaggcaggtc tgtcgccgtc tgctgcatga tatatccaag ccagtacctg aacgcttcct
     5581 ggttctttgc cagggctatt atctggcctg ccacctcaag gcttcccagg cccagcatca
     5641 ggaatatggg gaccacgacg cagatggcag caacaagcga cccgagcttt tttctcttgc
     5701 cgaaccggtc ccttatgggc cggcccacat aggcaaaaac gctgcccagg attatgccgt
     5761 ctaagaatgg aaaggcaaag catgcagtca tgaggaccgc ggccagggct aggattaaaa
     5821 tgcccttatg ctctgagcac cagcgatcag ggtcgtagtc catgggtcac accaaagaat
     5881 gaagcatctt gtcttgccgc ggaattcatt cgcttgatag atatgggtga cgctttatta
     5941 atgtttcaag ggaatgaaga ttgagtagga gagatgcgcc agagttgcat aaggagatga
     6001 tgactgagga tcgagcattc tccagggcca ggaaatagat gcttcttgtg acttcgcctg
     6061 ctactattgt tcgtcttttt tctaaccttg atcgtttctg tgcgtctctg tg
//
LOCUS       DAOSGP010000056         6034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11548_length_6034_cov_18.416792, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000056 DAOSGP010000000
VERSION     DAOSGP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11548_length_6034_cov_18.416792"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..566)
                     /locus_tag="PLV49_02740"
     CDS             complement(3..566)
                     /locus_tag="PLV49_02740"
                     /inference="COORDINATES: protein motif:HMM:NF039705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HOG97790.1"
                     /translation="MLEDQIFCPACGTDTDHTMIKSGQENLVRCNDCGTVHPVEVKRE
                     RLVNLKVIVNRDDRALPHFITIRANELLNVGDELLVDDPTKDVVMTQITSLETDRRVR
                     SALAETVKTVWARATDEVPLRITVFRGGKSHPLKITVPGDELLEVGEKRRVENFQFII
                     VKIKLRGEGFADAAEAKNIVRVWGREI"
     gene            711..923
                     /locus_tag="PLV49_02745"
     CDS             711..923
                     /locus_tag="PLV49_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97791.1"
                     /translation="MTKMDKDGRDRSGRGMDECCLIAESMIDELHQITISERMATPSG
                     GGRNRVSFKRKIMQQIRNARRLATVG"
     gene            1176..1859
                     /locus_tag="PLV49_02750"
     CDS             1176..1859
                     /locus_tag="PLV49_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97792.1"
                     /translation="MNSKSCSIPQSCSELEIDLKRLDRTLQAAHRSSIDIKDAYDFYV
                     LALKEFNKENLSDSFLYCDRANYELTSAVNEAKINIRGSRFHSLRTISYFFQLYGLYA
                     IVFAVLAILFFSMLIYQHPEAKILDVPLWSSFFAGLGASAQILTGVAEDLRRYGLATR
                     YKRLWYMAIPLISMVFGYMAYLISSSGLIALNDGIGDGVFSIMFICFLTGFLTKWIIN
                     RLSRLSRDI"
     gene            complement(1860..2546)
                     /locus_tag="PLV49_02755"
     CDS             complement(1860..2546)
                     /locus_tag="PLV49_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HOG97793.1"
                     /translation="MKSEIKEELNPERVKSEPLSVQDLHELYDPIKDQIEERLAEFQR
                     IWETASDEDLFREMVFCLLTPQSKARICWRAVERLERKCLIASGEPCQVQEELVGVRF
                     NQRKAEYVCLARSMFSHRSLRTTLAGFSSHAAAREWLVANVLGLGYKEASHFLRNIGL
                     GEDLAILDRHILKNLALLGVIDKVPESPTKRLYIEIEKQMTAFSSQAGIPMGQLDLLL
                     WYKEAGEIFK"
     gene            2631..4601
                     /gene="uvrB"
                     /locus_tag="PLV49_02760"
     CDS             2631..4601
                     /gene="uvrB"
                     /locus_tag="PLV49_02760"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOG97794.1"
                     /translation="MAEDSLSQHRPVEQQFRLHSSFAPCGSQPEAIDQLVLGLQRNER
                     FQTLIGVTGSGKTYTVANVIGQVQKPTLVIAHNKTLAEQLYNEFKEFFPDNRVEYFIS
                     YYDYYQPESYIPSKDQYIEKDSLINPKIEQMRLATTASLLSRRDVIVVASVSCIYGLG
                     NPENFQSMGFELKVNERISRKDILERLVDILYERSDLDLSPGRFRVNGETIDIIPGYF
                     NNIIRIELFGNKVERISEVDRITGKRVEDMSYFFVYPARHYVIPENELKAAVDSIKAE
                     LNERLQELGMIEAHRLRQRTMHDMEMIEETGSCKGIENYSRHFDRRQPGEPPYCLIDY
                     FPDDFLLIIDESHQTLPQLRAMNRGDRSRKKNLVDYGFRLPSSYDNRPLDFEEFERFM
                     RNVIFVSATPGDYELEHSKSPVEQIIRPTGLLDPEVEVRPIDGQVKDVMTEISGVISR
                     GDRALVTTLTKRLAEELTDYLSQNDIRARYLHSEIQPLERTEIIRELRLGKFDVLVGI
                     NLLREGLDIPEVGFIGILDADKEGFLRDEKSLIQTIGRASRNANSRVVLYADRMTGSI
                     KKAMETTERRRSLQMAYNQEHGIVPQTIKKPIREKVVEITDTRHIPKSDIPNAIIELE
                     AEMMAAAERLEFERAIQLRDTVRRLEREMKVA"
     gene            4677..>6034
                     /locus_tag="PLV49_02765"
     CDS             4677..>6034
                     /locus_tag="PLV49_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG97795.1"
                     /translation="MEAREEEAQKEITRGVSLLIGEPKKALLKLSGPMIVAMLILAAY
                     NLVNAVWVAGLGSDALAAVGFVTPIFMVVVGLSNGLGAGVSSSISRRIGAQDKRGADN
                     TTMHALLLVLLVSMILTAVLLLTLEPLLSAMGAGSSLGLAMEYGNIVFSGSIFMVFTN
                     IAYAILRGEGDTKRTMYAMGAGSLINAVLDPILIYWAGMGIAGAAWGTVISLLIVAMV
                     QIYWLIVKKDTYVSLSRNAFVPSRKVMSDILKVGIPASVEFLLYSIDAIIINSMLVRV
                     SGTDAVAVYTAGWRVIMMATIPLIAIATAEISVAGAAIGARRYGNLPVIHNYSTKLGF
                     AIGTVTALITGIFAPQITMFFTYTPESAHLAGTMVAFMHVMCLFYPFMSPGIMSACLF
                     QGAGKGLTSLLLNVLRDVVLITMVAFILGMVLGLGQEGIWWGIVFGNIAGSLLSYLWA
                     RL"
BASE COUNT         1456 a         1576 c         1560 g         1442 t
ORIGIN      
        1 aatcagatct cccgtcccca aacccgaacg atgtttttgg cctcagccgc gtcggcaaat
       61 ccctctccac gcagcttgat cttgactatg atgaactgga agttctccac cctccttttc
      121 tcgcccacct ctaagagttc atctcccgga actgtgatct taaggggatg gctcttgccc
      181 cctcgaaata cggttatcct cagcggcacc tcgtccgtag cccttgccca gacggtcttc
      241 acagtctcgg caagagcgct cctgacacgg cgatccgtct ccagagaagt gatctgggtc
      301 atcaccacat cctttgttgg atcgtccacc aaaagctcat ctccgacgtt cagcaactca
      361 tttgcccgga tggttataaa atggggcagg gcgcggtcat ccctattgac gatcaccttg
      421 aggtttacca aacgctctcg tttcacctca accggatgaa cagtcccgca gtcattgcag
      481 cgaactaggt tctcctgtcc ggatttaatc atggtatggt cggtatcagt gccgcaggct
      541 gggcagaaga tctgatcttc aagcatagaa tactgttgtc ctttgtggta ttaagcatga
      601 ccggaatgga tgagggaaaa tgagtcgaga tgatctgcac tgcttaatgc atctattttc
      661 agtttctatg caaggaaaaa ttttttaagt aagatgttca attaatactt atgacaaaaa
      721 tggataaaga tgggcgagat agatctggca ggggaatgga tgagtgttgc ctcatagcgg
      781 agagcatgat cgatgaattg catcagatca caatatctga gagaatggcc accccctcgg
      841 gtggcggaag gaacagagtc tcattcaaaa gaaagataat gcagcagata agaaatgcca
      901 ggaggttggc tacggtcggt tgaaaataat acgtgatact tctaaatgtt ttatataatt
      961 tatattctaa ataaaatctc tttcattcga aataacgaac atatttgatg aaaaatatat
     1021 tacacttaat aataatttct taaagagctt attcggagta taatcgggct atttgttatc
     1081 aatcacaaat tcaaatcatt taaaattatt taaattatgt gaaaagggct tgcgccgcgc
     1141 aaactataaa tattaattta gcgagttact atagtgtgaa ctccaaatca tgctccatcc
     1201 cgcagtcgtg ctctgagctg gagatagacc tcaagaggct cgaccgcacg cttcaggccg
     1261 ctcacagaag ctctatagac ataaaggatg cctatgactt ctatgtcctc gccttaaagg
     1321 agttcaacaa ggagaacctc tccgactcat ttctttactg cgatcgggcg aactatgagc
     1381 tcacaagtgc cgtcaacgag gccaagatca atatcagagg ctcccggttc catagcctga
     1441 gaacgatctc ctacttcttc cagctctacg gcctttatgc tatagtattt gcagtacttg
     1501 ccatcctatt tttcagcatg ctgatctatc agcatcccga agcgaagatt ctcgatgtcc
     1561 ctctctggtc ctcgttcttc gccggcctgg gggcatcagc tcagatcctc accggagtgg
     1621 cagaagacct gcgccgctac ggcctggcca cccggtacaa gaggctctgg tatatggcca
     1681 tacccctgat cagcatggtc ttcggttata tggcctatct gatatctagc tctggactga
     1741 tagcgctgaa tgacggtatc ggagatgggg tcttcagcat aatgttcatc tgctttttaa
     1801 ccggtttcct gaccaaatgg ataataaaca ggctctcgcg actgtccaga gatatttgat
     1861 tacttgaata tctctcccgc ttccttgtac cagagcaata gatcaagctg gcccatcgga
     1921 attcctgcct gggaagagaa ggcggtcatc tgcttctcga tctcaatgta aagcctcttg
     1981 gtgggcgatt cgggaacttt gtctatcact cccagcaggg ccagattttt gaggatatgc
     2041 cggtcgagaa tggccagatc ctcccccagg cctatgttcc tcaagaaatg gctggcctcc
     2101 ttatagccaa ggcccaggac gttcgccacc agccactccc gggcagcggc atgactggaa
     2161 aatccggcca gagtggtgcg aagggatcga tggctgaaca ttgaccgggc aagacataca
     2221 tactccgcct tccgctggtt gaacctcacg cccaccagct cttcttgaac ctggcatggc
     2281 tcaccagaag cgataaggca ctttctctcc agcctctcca ccgccctcca gcatatcctg
     2341 gccttggact gaggggtgag aaggcagaac accatctcac ggaagaggtc ctcgtcgctg
     2401 gccgtctccc atatgcgctg aaactctgcc agtctctcct cgatctgatc ttttattgga
     2461 tcatagagct catgcagatc ctggacggag aggggctcgc tttttaccct ctccgggttt
     2521 aattcttctt tgatttctga tttcatccct cttgacctct gtctccggat tatttagcca
     2581 ttgtgcaggg caaaagctat tagcaccaag gattatgcct gccaatgagc atggcagaag
     2641 attcattatc ccagcaccgt ccggttgagc agcagttccg cctccactcc agctttgctc
     2701 cctgcggctc ccagccggag gccatagatc agcttgtttt gggcctgcaa aggaatgaga
     2761 ggttccagac tctgatcggg gtcacgggct caggcaagac ctatacggta gccaatgtca
     2821 ttggacaggt tcagaagcct acccttgtga tcgcccacaa caagaccctg gctgagcagc
     2881 tttacaatga gttcaaggag ttcttcccgg ataaccgggt tgaatatttc atctcttatt
     2941 atgactacta tcagccggag tcgtatatcc cctcaaagga tcagtacata gagaaggact
     3001 ctctcatcaa tcccaagata gagcagatga ggctggccac caccgcctcg cttctctccc
     3061 gccgggatgt gatagtagtg gcctccgtct cctgcatcta cggcctgggc aatcccgaga
     3121 acttccagag catggggttt gagctgaagg tgaatgaaag gatctccaga aaggatatcc
     3181 tggaaaggct ggtggacatc ctctatgagc gctcagacct ggacctctct ccgggacgct
     3241 tccgggtcaa cggcgagacc atcgatatca tccccggcta cttcaacaac atcattcgta
     3301 ttgagctctt tggaaataaa gtagagagga tctctgaggt cgacaggatt accggcaaga
     3361 gagtcgagga tatgagctac ttcttcgtct atccggccag gcattatgtc ataccggaga
     3421 acgagctgaa agcggcggtc gactccataa aggcggagct gaacgagagg cttcaagagc
     3481 ttgggatgat cgaggcccac cggctccggc agagaacaat gcatgatatg gagatgatag
     3541 aagagaccgg aagctgcaag ggaattgaga actactcccg ccattttgac cgccgccagc
     3601 caggagagcc tccatactgt ttgatcgact attttcccga tgactttttg ctcataatcg
     3661 acgagagcca tcagaccctc ccccagctca gggcgatgaa cagaggcgac cgatcccgca
     3721 agaagaacct ggtggattat ggcttccggc tgcccagcag ctatgacaac cggccgcttg
     3781 attttgagga gtttgagcgg ttcatgagaa atgtgatatt cgtctccgcc accccaggag
     3841 actatgagct cgagcattcg aaatctccag tggagcagat catccgcccc accggccttc
     3901 tcgatccgga ggtggaggtc aggcccatcg acgggcaggt gaaggatgtg atgacggaga
     3961 tctccggagt gatctcgaga ggggatcgag cactggttac caccctcacc aaacggctgg
     4021 cagaggagtt gaccgattat ctgagccaga atgatattcg ggcccgctat ctgcattcag
     4081 agattcagcc tctggagagg acggagatca tccgtgagct gaggctgggc aaattcgatg
     4141 tcctggtggg aatcaacctc ctccgagagg ggctggacat ccccgaggtg gggttcatag
     4201 gaattttaga cgcagacaaa gagggattcc tccgggatga gaagagcctg atccagacca
     4261 tcggccgggc ctcaaggaac gccaactccc gggtggtcct ctatgctgac cggatgaccg
     4321 gctccataaa gaaggcgatg gagaccacgg agaggcgcag atctctacag atggcataca
     4381 accaagagca cggcatcgtc ccacagacca tcaagaaacc catccgggaa aaggtggtgg
     4441 agatcaccga caccaggcat attccaaaat cggatattcc aaatgcgatc atcgagctgg
     4501 aggcggagat gatggcagcg gcggagcgtc tggagttcga gagggccatt cagctccggg
     4561 ataccgtccg caggctggag agggagatga aggtggcttg aaaagagacc ataagccgca
     4621 tctccctttc cagagctgtg aattcaaagg gccccagtga tcggatgagc gatgggatgg
     4681 aagcgaggga ggaagaggcc caaaaagaga ttaccagagg ggtttccctg ttgatcggag
     4741 agccgaaaaa ggccctcttg aagctctccg gccctatgat cgtcgccatg ttgatccttg
     4801 ccgcctacaa tctggtgaac gccgtctggg tggcaggcct gggatcggat gccctggctg
     4861 cagtaggatt tgtgaccccc atattcatgg tggtggttgg cctgagcaac ggcctggggg
     4921 caggggtttc ttcttccatc tcccgcagga taggggcaca ggacaaaaga ggggcggata
     4981 ataccaccat gcatgccctc ctgctggttt tgctggtctc aatgatactg acagcagtcc
     5041 tcctcttaac cctggagccc ctcctttccg caatgggtgc aggctcctct cttggtctgg
     5101 ccatggagta cggcaatatc gtcttttccg gatccatatt tatggtcttc accaacatcg
     5161 cttatgccat cctgagagga gaaggggata caaaacgcac catgtacgcc atgggcgcgg
     5221 gatcgctgat caacgccgtc ttggatccca ttctgatcta ctgggcgggg atggggatag
     5281 caggagcagc atggggcaca gtcatctctc tcttgatcgt ggccatggta cagatctact
     5341 ggctgatcgt caagaaggac acctacgtct ccctgtcccg gaatgccttt gtccccagca
     5401 gaaaagtgat gagtgacatc ctcaaggtgg ggattccggc cagcgtcgag ttcctccttt
     5461 actccataga tgccatcatc atcaacagca tgctggttcg ggtttcgggg acggatgctg
     5521 tagctgttta tactgcaggc tggcgggtga taatgatggc caccattcct ctgatcgcca
     5581 ttgccacagc agagatctct gtggcaggag ctgcaattgg cgccaggaga tatggcaacc
     5641 tgccggttat tcataactat tccacaaagc ttggcttcgc cattggaacg gtcaccgccc
     5701 tcatcaccgg catatttgcc cctcagataa ccatgttttt cacctatact cccgagagcg
     5761 ctcatcttgc cggaacgatg gtggcattca tgcatgtgat gtgcctgttc tacccattca
     5821 tgtccccggg aataatgtcc gcctgcctct tccagggggc gggcaaaggt ctcacctccc
     5881 tcttgctcaa tgtcctcaga gatgtggttc tcattacaat ggtagccttc atcctgggaa
     5941 tggtcttggg gctgggtcag gaggggatat ggtggggaat cgtgtttgga aacatagcag
     6001 gaagcctcct gagctattta tgggccaggc tcta
//
LOCUS       DAOSGP010000057         5991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11674_length_5991_cov_16.754212, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000057 DAOSGP010000000
VERSION     DAOSGP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11674_length_5991_cov_16.754212"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLV49_02770"
     CDS             complement(<1..242)
                     /locus_tag="PLV49_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiA"
                     /protein_id="HOG97796.1"
                     /translation="MTNQGFSDFRAYIELLRPPLAPMDIAMPAASALLAVYAVEGTLP
                     PLIPFVIAVLGAYCAITSSYVYNDCCDIEVDKVGMP"
     gene            complement(290..1009)
                     /locus_tag="PLV49_02775"
     CDS             complement(290..1009)
                     /locus_tag="PLV49_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HOG97797.1"
                     /translation="MDLEFGGKTKEPDIRRLFDMKDVIFDQSWLAGRENSELYYMFRD
                     LFLSRADGDKLRDQNLRYDITIIPPGMMGCEFIKTAGHYHPQVPGSDVTYPELYEVLE
                     GEALYLLQKEDLSDVVAINASAGDKVLVPPNYGHITINRSNKTLKMSNFVARDFSSLY
                     NPMKEKGGGAYFFTRDGWIKNPRCPEASELRRADAPGGRTLSQLGLAKGKEMYPLLKE
                     HGRLDYLTRPGDHLDLFSGVI"
     gene            complement(1164..1613)
                     /locus_tag="PLV49_02780"
     CDS             complement(1164..1613)
                     /locus_tag="PLV49_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97798.1"
                     /translation="MRTRVFALVALALLLMGGGALASHPMFFPLSEHTIAKDITDSGM
                     PLYRTTTFTTDDEQAVSWLLIWRDSKPHTVEWRWYWPEGRTYARSFSTIPPIDGFTGM
                     WAAPVWSCLKIKGTDVARLPGNWRVDVIVDFKKELTEYFTINTPYAC"
     gene            complement(1726..2370)
                     /locus_tag="PLV49_02785"
     CDS             complement(1726..2370)
                     /locus_tag="PLV49_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HOG97799.1"
                     /translation="MKAKTAKALEEARYLVDRGYPKDSAVRFISNHHRLAIEERFIMM
                     RVIVRSDIAQIRRSRMQKLEDLKDQTIFIDGYNVLITTESLLGGHPVYSCDDGFLRDS
                     RGIFRSYKISSLTALALNQILDLLSQAGPARTEVLLDQQMSRSGEMAEMIKGMMAERH
                     LCGTARTARDVDRLLKATRGIVLTSDGNVIDSLDHVMDLPREIARRSGIRPLTL"
     gene            complement(2367..2903)
                     /locus_tag="PLV49_02790"
     CDS             complement(2367..2903)
                     /locus_tag="PLV49_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOG97800.1"
                     /translation="MTIFHLHTPLCQEDVKKLNAGDIVFLKGKVFTARDKAHALIRRQ
                     GSPVPLEGAALYHCGPIIQDDSVISAGPTTSGRMARYTEEILALGVKAIIGKGGLPGE
                     PFLNRAVYLAYPGGCGAAAASQLRIRAVHLPELGMAESIWELEAEDLGPMVVAVDSYG
                     RDLYQDVRLSAKAGIEKR"
     gene            complement(2900..3784)
                     /locus_tag="PLV49_02795"
     CDS             complement(2900..3784)
                     /locus_tag="PLV49_02795"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOG97801.1"
                     /translation="MITSSQVTDATVRALYRAETSLPPWVLERIKEGLERETNPLARS
                     QLQFMLDNVSLAGSRGLPICQDTGLAIFHVQMGQDLHLDFDLQEAIAQGVRRATSQIP
                     LRPNAVDPVTRENSKDNTGPGMPDIILDRTCGQSLSITAFPKGAGSENMSLVMMLNPA
                     DDPMEFIVDAVRERAANACPPLFLGIGIGGSFDLAARLAKHALLNMHRLQPYSRESSQ
                     RGDCCITLNRSPEEIKKLEENLMKRLNSLGIGPMGLGGDTTVLGLRIEKALCHTASLP
                     VAINFQCWANRSATEVIL"
     gene            complement(3805..4545)
                     /locus_tag="PLV49_02800"
     CDS             complement(3805..4545)
                     /locus_tag="PLV49_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF166 domain-containing protein"
                     /protein_id="HOG97802.1"
                     /translation="MLVGVIKRGKYGERLLETIKDHTDFEVVSVDVPEILPGFIEDPE
                     EFVRELNLNPQIFQADLLILYTFHPDLTPEIVRLAGKEGVKAVIIPGGIGRAGSITEL
                     EKIAEEYNIHIEVDEICCTLEECGVPAIDQFAKKLGKPELEVQTKDGKISGVRVLRGS
                     PCGATWHAATDIVGKTVAEAPSFTGLFCQQYPCRAVRGGPGGIHTSGDLHKDAMERAL
                     GKVTRLVLPEQSKPIKIEPGKKGKKLEG"
     gene            4636..>5991
                     /locus_tag="PLV49_02805"
     CDS             4636..>5991
                     /locus_tag="PLV49_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOG97803.1"
                     /translation="MEGLSEKSSESSRIRSVPAVLVAGTHSGVGKTTITLGLMAALRA
                     RGLVVQPYKVGPDFIDPSHHTAICGRPSFNLDPFMMSEEGVRRSFFSALEGADVAVVE
                     GVMGLFDGMDGTEVGSTAQVAKILDIPVLLVINVHGMSRSAAAMELGFASYDPGVRLA
                     GTILNRVGSKRHLDMLKSCLAKPVFGALPRDKKMEMKSRHLGLVMSFEKDHDPEILAT
                     FLEENADIDMILKLPGQARTPAISDAIELAVAKADGNGSVRIGVARDRAFCFYYHENL
                     LELERHGADLVYFSPLEDKLPDVDGLYIGGGYPELYASQLERAPARKQIKKASEDGMP
                     IYGECGGLMYLGAALESEAGSFKMAGALPCTTFMEKRLMALGYVKAEVSERNPLADVG
                     RSLRGHEFHYSRCICDRDARYCLKLSRGKGIADGRDGLVENNTMAGYLHTHFYSVSGN
                     KF"
BASE COUNT         1296 a         1625 c         1644 g         1426 t
ORIGIN      
        1 cagggcatgc cgaccttatc cacctcgata tcgcagcagt cgttatagac ataagagctg
       61 gtgatggcac agtaggctcc gagcacagct attacaaaag gaatcagtgg ggggagagtc
      121 ccttccaccg catagaccgc caagagagcc gaggctgcag gcatggcgat gtccatgggc
      181 gcgagcggag ggcgtagcag ctcgatatat gctctgaaat ctgagaatcc ttggtttgtc
      241 attttattca aatttccctc agggggttca ttcttcgaat tccatgctat cagattacgc
      301 ctgaaaagag atccaggtga tccccgggcc gggtcagata atccagccgg ccatgctcct
      361 tgaggagagg gtacatctct ttgcctttgg ccagccccag ttgggaaagg gttctgccac
      421 ctggtgcgtc ggcccgccgc agctcagacg cctccggaca gcgcgggttc ttgatccaac
      481 catcgcgggt aaagaaatat gccccccctc ccttctcctt catgggattg tagagggatg
      541 agaagtccct ggccacgaaa ttggacatct tgagggtctt attggatcga ttgatggtga
      601 tatgcccgta attggggggg accagaacct tatccccggc agatgcattt atggccacca
      661 catcggatag gtcctccttc tgcaggagat agagcgcctc accctcgagc acctcataga
      721 gctcaggata ggtgacgtca gaccccggca cctgtggatg gtagtggcct gcggtcttaa
      781 tgaactcgca acccatcatc cctggaggga ttatggtgat atcgtatctc aagttctggt
      841 ctcggagctt atccccgtcc gcccggctca agaataggtc gcggaacata tagtacagct
      901 cagagttctc tcttcccgcc agccagctct gatcgaatat aacgtctttc atgtcaaata
      961 gcctgcgaat atcaggctct ttggtcttgc caccaaattc tagatccatt ttctatgcca
     1021 ccgctctcct taggccctcc aggatttata tggtcttcca caacgaaatg aaaagaccaa
     1081 aagcaaagct aacgctttag cttgccagtc ttatcattca ggacctttat taatctcgat
     1141 atgctgatat gccagtatag gactcagcag gcataaggag tgttgatggt gaagtactcc
     1201 gtgagctcct ttttgaagtc cactatcaca tccaccctcc agtttcctgg aagcctggca
     1261 acgtcggttc ccttgatctt caggcagctc cagacgggtg ccgcccacat tcccgtgaat
     1321 ccgtcgatgg gcggaatggt ggaaaacgac cgggcatagg tcctgccctc gggccagtac
     1381 catctccact ccacagtatg cggctttgag tcccgccaga taagcaacca ggagacagcc
     1441 tgctcatcat cggtggtaaa tgtggttgtc ctgtatagcg gcatgccgct atcggtaata
     1501 tccttggcta tggtatgctc gctaagggga aagaacatag gatgggatgc cagagcccct
     1561 ccgcccatga gcaacaatgc caacgccact aaagcaaata ctctcgttct cattaccact
     1621 caccataact ttgaaagctt ttcaaacccc aatagtatat taatccatgt agcataaaaa
     1681 gataacgatt ggatgataga gataaaagat ggtgtcaggc aattgtcaga gagtcagagg
     1741 ccttatgccc gatcttctgg caatctctcg gggaaggtcc ataacatgat ccaagctatc
     1801 gatcacattt ccgtcgctgg tcaacactat tcccctggtt gccttaagaa ggcgatcgac
     1861 atctctggcg gttctggccg tgccgcagag atgccgctct gccatcattc cttttatcat
     1921 ctctgccatc tctccgctgc ggctcatctg ctggtccagc agcacctctg ttcgggcggg
     1981 accggcctgg gacaggagat caaggatctg gttgagggca agagcggtga gcgaggagat
     2041 tttgtagctc ctgaagatcc ctctgctatc tcgcaggaag ccgtcatcgc atgaatagac
     2101 gggatggcct ccgaggaggc tctcagtggt gataaggaca ttgtagccgt caatgaagat
     2161 ggtctgatcc ttcaggtcct ccaacttctg catcctggac cttctgatct gagcaatgtc
     2221 cgatctcact attaccctca tcatgatgaa cctctcttca atcgccaatc gatggtgatt
     2281 ggagatgaac ctgaccgcag agtccttggg gtagccccgg tccaccagat acctcgcttc
     2341 ctccagggct tttgccgttt ttgcttttat cgcttctcta ttccagcctt cgccgacagt
     2401 ctcacatcct ggtacagatc ccgaccatac gagtccactg ccaccaccat cggaccaaga
     2461 tcttcggcct ccagctccca gatggactcc gccatcccca gctccgggag atggacagca
     2521 cgaatccgaa gctgtgatgc agccgccgca ccgcaacccc ctggataggc aagatagact
     2581 gctctattca agaacggctc tcctggaagg cctcctttgc cgattatggc cttcacccct
     2641 aaagccagaa tctcctcggt atagcgcgcc attcggccgc tggtggtagg cccggcagag
     2701 atgacagaat catcctgaat gatggggccg caatgataga gcgccgctcc ctctaaaggt
     2761 actgggctgc cctggcggcg gatcagagca tgggctttgt cccgagcggt gaacaccttg
     2821 cccttcagaa atactatatc accagcgttt agctttttca catcctcctg gcaaaggggg
     2881 gtatggagat gaaatatggt cataaaatca cctcggtagc gcttctgttt gcccagcact
     2941 ggaagtttat ggccaccggc agggaggcgg tatggcatag agccttctcg attctcaggc
     3001 cgaggacggt ggtgtccccg cccaggccca tggggccgat gcccaggctg ttcagcctct
     3061 tcatgaggtt ctcttcaagc ttttttatct cttcaggcga gcggttaagg gtgatgcagc
     3121 agtcgcctct ctgagaggat tcccgagaat atggctggag gcggtgcata ttgaggagag
     3181 cgtgcttggc cagccgggcg gccagatcga agctgccgcc gatccctatt cccaggaaca
     3241 gaggtggaca ggcattggct gccctctccc tgaccgcatc gacgatgaac tccattgggt
     3301 cgtctgccgg attgagcatc atgacgaggc tcatgttctc agatcccgct cccttgggaa
     3361 aagcggttat gctcaggctc tggccacagg tccggtccag gatgatgtcc ggcatcccgg
     3421 gaccggtatt gtccttgctg ttctctcttg ttacgggatc gactgcattg ggtcggagag
     3481 gtatctggga tgttgctctc ctcactcctt gagctatggc ctcctgaaga tcgaagtcca
     3541 ggtgcaggtc ttggcccatc tgaacatgaa atatagcaag gcccgtgtcc tggcagatgg
     3601 gcaagcctct tgatccggca aggctaacat tgtccagcat gaactggagc tgagagcggg
     3661 ccaggggatt ggtctccctt tcaagccctt cttttattct ctccaagacc cagggaggaa
     3721 gagaggtctc agctcgatat agggcgcgaa cggtggcatc tgtcacctgg gagctagtta
     3781 tcatctgatt ctcacccatc tcctttaccc ttccaacttc ttgccctttt ttcccggttc
     3841 gatcttgatg ggtttggact gctcgggaag aacaagccgg gtgaccttcc ccagagctct
     3901 ctccatggca tccttatgca ggtctccgga ggtgtggatg ccccctggac ctcccctcac
     3961 tgcgcgacat gggtactgct ggcagaatag gccggtgaag gacggtgcct cagccacagt
     4021 ctttcccact atatctgtgg cggcatgcca tgtcgctccg cagggggagc cgcgcaagac
     4081 gcgcacaccg ctgatcttgc cgtcctttgt ctggacctct agctccggtt ttcccagctt
     4141 tttcgcgaac tggtcgatcg ccgggacgcc gcactcctcc agggtgcagc agatctcatc
     4201 aacctcgata tggatgttat actcctcagc gatcttctcc agctcagtga tggagcctgc
     4261 ccggcctatg cctccgggaa tgatgaccgc cttaacccct tcttttcctg caaggcggac
     4321 gatctccgga gtgaggtccg gatgaaatgt gtagagaatg agcaggtcag cctggaagat
     4381 ttgcgggttt aaattcagct cccggacgaa ctcctccgga tcctctatga atcccggcag
     4441 gatctccggg acatcaaccg ataccacttc gaagtcggta tggtctttta tggtctctag
     4501 aaggcgttcg ccgtattttc ctctcttgat taccccaacc agcatgaaga agggatcagc
     4561 cagcgtatta atatccttgc cggagaagtg ggactcatgc gcccggcaag ctcggagaga
     4621 tggttggata gatctatgga aggattatca gagaaatcat cggagagctc acggataaga
     4681 tcggttccag cggtgctcgt cgccggcacc catagcggag taggcaagac gacgatcacc
     4741 ttaggtctga tggccgccct gcgtgctcgg ggtctggtgg ttcagcctta taaggtgggc
     4801 ccggacttca tcgatccctc ccaccatact gctatctgtg gacggccttc tttcaattta
     4861 gaccccttta tgatgagcga ggagggtgta cgtcgctcct ttttctctgc cctcgaggga
     4921 gcagatgtgg cggtggtaga gggtgtgatg ggtctattcg acggcatgga tggcacagag
     4981 gttggcagca ccgcccaggt ggccaagata ctggatatac ccgttctcct ggtgatcaat
     5041 gtgcacggca tgtcgcgaag tgctgccgcc atggagctgg gttttgccag ctatgatcca
     5101 ggggtgcggc tggcggggac gatactcaac cgggtgggaa gcaagaggca tctggatatg
     5161 ctcaaaagct gcctggcaaa gcccgtattc ggagctctcc ccagggataa aaagatggag
     5221 atgaaaagcc gccacctggg actggttatg agctttgaga aggatcatga tccagaaatc
     5281 ctggcgacat tcctggagga gaatgccgat atcgacatga ttctcaagct tccgggccag
     5341 gcccgaacgc ccgcgatttc cgacgccatt gaactggcag tggcaaaggc agatggaaat
     5401 ggatcggtgc gaataggagt ggccagggat cgagccttct gcttttacta tcatgagaac
     5461 ctcttggagc tggagaggca tggcgcggat ctggtctatt tcagccctct ggaagacaag
     5521 ttgcccgatg tggatggact gtatatcggg ggaggctatc cagagcttta tgcctctcag
     5581 ctggagaggg ctcctgccag gaagcagatc aagaaggctt ccgaggatgg tatgcccatc
     5641 tatggagagt gcgggggtct gatgtatctg ggagcagccc tggagtcgga ggcaggctcc
     5701 tttaaaatgg ctggggccct tccctgcacc acgttcatgg agaagaggct catggccctt
     5761 ggctatgtga aggcggaggt atcggagaga aatcctctgg ctgatgtggg caggagcctt
     5821 cgcggtcatg agtttcacta ttcgcgctgc atttgtgata gggatgcccg ttattgcctg
     5881 aagctttccc ggggaaaagg aatcgctgat ggccgggacg gtctggtgga gaacaacacc
     5941 atggctggat atctgcatac tcacttttat tcggtatctg gtaataaatt c
//
LOCUS       DAOSGP010000058         5977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11706_length_5977_cov_18.996623, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000058 DAOSGP010000000
VERSION     DAOSGP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11706_length_5977_cov_18.996623"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLV49_02810"
     CDS             complement(<1..602)
                     /locus_tag="PLV49_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HOG97804.1"
                     /translation="MFLGVDHGTTAIRFASEKGSIYELSRSSAADLSYEQILSRIEES
                     LGPERVDLVALSYSMGDGLTKIVRLEDAPNRGLISQDGAGLHQGGGTRVFEAIRASGW
                     PAILLPGIHRASNVDPRMKVFSHGMSPEKVGLAYGVFRQGSSSFIVCDASSNTVSFAV
                     LRGEIVAAIDAPIFAPGLLQGPLDVQAIRDVDSGKLTANQA"
     gene            complement(593..1486)
                     /gene="cofD"
                     /locus_tag="PLV49_02815"
     CDS             complement(593..1486)
                     /gene="cofD"
                     /locus_tag="PLV49_02815"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HOG97805.1"
                     /translation="MLVLSGGTGTPKLLRGLKELVLPQELSVAVNTAEDLWISGNYIS
                     PDIDSVLYTLADMIDEKRWWGIKGDRTLTHDFLLMMGVDEKLTIGDKDRAVHIFRSNL
                     LRRGVKLSLTTEAIANALGVMQRVVPMTDDIVSTVIGTPEGMMHFQDFWASLKGRPRV
                     ESVTFTGLEEALPCQGFLDLLEREETVIIGPSNPVTSIGPILALAGVRDRLKKKKVVA
                     ISPFVGNKPVSGPAAKLMEARGVPASDEGVAALLGKVDIFVVDGKSDYTGDCVRMKTL
                     MRTKNESLAMAKGLLDLIGCS"
     gene            complement(1488..2057)
                     /locus_tag="PLV49_02820"
     CDS             complement(1488..2057)
                     /locus_tag="PLV49_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HOG97806.1"
                     /translation="MNLTTNANCPVCSAQMQFSWETQDIPYFGEAMLIAGVCECGFRH
                     SDTILLTQKEPVRNTLVVETIEDLNARVIRSSSGTIRVPELGVDVEPGYASDSYVSNV
                     EGVLDRIEEIVCFATSQARAADNQDGARRGEEILESIALARKGEFPITLIIEDPLGNS
                     AIASEKVKVSPLTDEDMARLQTGIMLLEV"
     gene            complement(2054..2419)
                     /gene="sepF"
                     /locus_tag="PLV49_02825"
     CDS             complement(2054..2419)
                     /gene="sepF"
                     /locus_tag="PLV49_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOG97807.1"
                     /translation="MAGFLEKLKLIGKSSEQDYIEVDLSQFEEPRGSNEIILRVAEMG
                     RIEVLPEVKQEIYNGNIVLADIAGLKRDKAALDRAIGELKKAVEDVSGDIAGIGDDLI
                     VLVPKGIKIDREKVVGGKD"
     gene            complement(2472..2651)
                     /locus_tag="PLV49_02830"
     CDS             complement(2472..2651)
                     /locus_tag="PLV49_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97808.1"
                     /translation="MKKKYLEIGLSTGLVLLMIILILGAQMTLPAGERGSSFAIIILL
                     FIVAMGIVGLKLDDM"
     gene            complement(2657..3658)
                     /gene="cofG"
                     /locus_tag="PLV49_02835"
     CDS             complement(2657..3658)
                     /gene="cofG"
                     /locus_tag="PLV49_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofG"
                     /protein_id="HOG97809.1"
                     /translation="MKRITYSKNIFLPVTNLCRNRCQYCSFRRNLDEAHLISRSAAQQ
                     LLEEGRDHGCSEALFTLGERPEEVEGFHLMLAGEGREDLLGYLVELCELAIEHDLLPH
                     TNAGLLSEEDLATLQPYNASMGLMLESCATLNAHEQSPGKDPELRLKHIARAGRLHIP
                     FTTGILVGIGESWQDRIQSIQAISRLHDEYGHIQEVIVQPFDPKPGTAMSTHPRPERE
                     DLVRTVRLARELLPREVAVQVPPNLADPLPLIEAGADDLGGISPITRDEINPNSSWPR
                     EEELKRSLFAYELKERLPVYPRFIRRGWHGSKTRRLTAALASEDGLRRKSISSREWW"
     gene            complement(3659..4108)
                     /locus_tag="PLV49_02840"
     CDS             complement(3659..4108)
                     /locus_tag="PLV49_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HOG97810.1"
                     /translation="MSEASTQITLIGTKLASIGMEFTFMGPTPECESCKLRNTCINLE
                     PRRRYRVLGTRGELVHECPIHEAGVRAVEVAESPIIAAFDARKAFPGSKIVFEAMKCD
                     DYNCSMYDICHPIGLKDGEKCTIVEVVGEAPEDCPRGNILKLVEFRR"
     gene            complement(4178..5209)
                     /locus_tag="PLV49_02845"
     CDS             complement(4178..5209)
                     /locus_tag="PLV49_02845"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HOG97811.1"
                     /translation="MELPRIVAVGNEAILELPQICSYLHLEGPGLIVTGPHTDDVVGK
                     TILELLNSGGFEPQTLICTASKLEEVDRAEARAREIEAGFLIGAGGGRSIDIAKIASM
                     RTNIPFLSLPTAASHDGICSSQASLTVNGEAVSLPAQSPLAIVADTGIIARAPARLLA
                     AGCGDIISNYTAILDWQLAHRLRSEEFSEYASALSGMTAKMIVERASDIRPGLEESAK
                     IVVKALISSGVAMSIAGSSRPASGSEHKFAHSINRIAPRRGLHGELCGLGTIMMMYLH
                     GKDWKMIREALAQVGAPTSADQIGLDAETVVEALVNAHRIRPERYTILDSGLNRDAAA
                     RAAEATGVI"
     gene            complement(5290..>5977)
                     /locus_tag="PLV49_02850"
     CDS             complement(5290..>5977)
                     /locus_tag="PLV49_02850"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG97812.1"
                     /translation="DINLTIEENQFVCFIGPSGCGKTTLLRIVAGLEAPTSGAATIDG
                     EPIVGPSPERGMVFQEYSLFPWRTILDNTVFGLELKGIDKAKREERGRQYLKMVGLEG
                     FEKRYPHELSGGMKQRVAIARALVNDPKALLMDEPFGALDAQTRNTMQSELLRIWEEE
                     KKTVLFVTHSVDEAIYLADAIVIMSARPGRIKDIIQIPLPRPRTRTSTEVNQIRDRIL
                     CDLRTEIASC"
BASE COUNT         1202 a         1797 c         1541 g         1437 t
ORIGIN      
        1 gcctggtttg cggtcagctt tccggagtcg acatctctta tcgcctgaac gtccagggga
       61 ccctgcagga ggccgggtgc gaagataggt gcatctatgg ctgccacgat ctcgcccctc
      121 agcacggcga agctgacggt attggagctg gcgtcgcata cgatgaagct ggagctaccc
      181 tgccggaaaa ccccataggc gaggcccacc ttctccgggc tcatgccgtg gctgaagacc
      241 ttcatcctgg gatcgacatt gcttgcgcga tgaattccgg gcaggagtat ggccggccag
      301 cctgaggccc tgatggcctc gaatacccgg gtaccccctc cctgatgcaa gcccgcacca
      361 tcctgactta tcagcccccg gttgggtgca tcctccagcc ggactatctt tgtcaggccg
      421 tcgcccatgg agtaggagag ggcaaccagg tccacccttt ccgggcccag gctctcttca
      481 atccgggata atatctgctc gtaagaaaga tcagcggccg agctgcgcga gagctcatag
      541 attgagccct tttcactggc aaacctgata gctgtggtgc cgtggtctac gcctaagaac
      601 atccgatcaa atccagcaag cctttcgcca ttgccagact ctcatttttt gtcctcataa
      661 gggttttcat gcgcacgcag tccccagtgt agtcgctctt tccgtcgacc acaaagatgt
      721 ctaccttgcc taaaagtgct gccactcctt catcgctggc cggaactccc cgggcctcca
      781 tgagctttgc tgccgggccg ctaacgggct tatttcctac aaacgggctt atggccacta
      841 ccttcttttt cttcaggcga tctcgcactc cagcaagggc gaggattgga ccgatgctgg
      901 tgaccggatt gcttggaccg atgatgacgg tctcctccct ctccaagaga tccaggaatc
      961 cctggcaggg gagggcctct tccaatccag tgaaggtcac ggattcgacc ctcggccttc
     1021 ccttgaggct cgcccaaaag tcctgaaagt gcatcatccc ctccggggtg ccgataaccg
     1081 tggatacgat gtcatcggtc atggggacca ccctctgcat gacccccagg gcattggcta
     1141 tggcttctgt ggtcaggctg agcttgactc cccggcggag gagattggag cgaaatatgt
     1201 gcaccgcccg gtccttgtcc ccaatggtca gcttctcgtc cactcccatc atgaggagga
     1261 agtcgtgggt taatgtccgg tcccccttaa tgccccacca tcgcttctca tcgatcatat
     1321 ccgccagggt ataaaggacg gaatcaatat ctggcgatat atagtttcca gaaatccaga
     1381 ggtcttcggc ggtgttcacc gcaacagaga gctcttgggg cagaacgagc tcctttaagc
     1441 ctctcaggag cttgggggtt cctgtgcctc cgcttaagac cagcatatca gacttccaaa
     1501 agcatgatgc ctgtctggag gcgggccatg tcctcatccg tcaggggcga tactttcacc
     1561 ttttctgagg ctatggcgct gttccccagt ggatcctcga tgattagggt tatgggaaac
     1621 tctcctttgc gagcgagggc gatgctctcc aggatctctt ctcctctccg agcgccatcc
     1681 tggttatctg ccgcccgggc ctgggaggtg gcaaaacaga ctatctcttc gattcggtcc
     1741 agcacccctt ccacgttgga gacatatgag tcggaggcat accccggctc tacgtccacc
     1801 ccaagctctg gcactcgaat cgttccgctt gatgagcgga tgaccctggc gttcagatct
     1861 tctattgtct ccaccaccag ggtatttctc accggctcct tctgggtcaa aagaatggta
     1921 tcgctatgcc ggaagccgca ttcgcagacc ccggcgatca gcatggcctc gccgaaatat
     1981 ggtatatcct gcgtctccca agagaactgc atctgggcag agcatacagg gcagttggca
     2041 ttggtcgtga gattcaatcc tttcctccga ccactttttc tcggtcaatc tttatgccct
     2101 ttggaaccag aactatcagg tcgtcgccga tgccagcaat atctccggat acatcctcga
     2161 ctgccttctt cagctcaccg atcgctctgt ccagggcggc tttatccctc ttcaggccgg
     2221 cgatgtccgc aaggactata ttgccgttgt agatctcctg cttaacctca ggcagaacct
     2281 caatccgtcc catctccgcc acccgcagga tgatctcatt cgagcccctg ggctcttcga
     2341 actggcttaa atccacctct atgtaatcct gctcgctgga ctttccaatg agcttgagct
     2401 tttccagaaa tcctgccatt taagaatccc tcaaagaaaa atgtgtatta ttatatttat
     2461 atttaacgca gctacatatc atccagcttc aatcccacga tgcccatggc cacgatgaag
     2521 agcaggatta ttatggcaaa gcttgatcct ctctctcctg cggggagggt catctgcgcc
     2581 cccaaaatga ggatgatcat caagagcacc agaccggtgc tcagccctat ctcaagatat
     2641 ttcttcttca caaaactcac caccattccc tggaagatat actttttcgc cgcagaccgt
     2701 cctcgctggc cagggcagca gtcagccttc gggtctttga gccatgccac cctctcctta
     2761 tgaaccttgg gtatacgggc agcctttcct tcagctcata ggcgaaaagg ctccttttca
     2821 gctcctcttc cctcggccag gagctatttg gatttatctc atctcgggtt atgggactga
     2881 tcccccccag atcgtccgct cctgcctcta tcaggggcag agggtcagcc aggttgggcg
     2941 ggacctggac cgctacctct cgagggagta gctcgcgggc aagtcttacc gtccggacca
     3001 gatcctcccg ctcgggccgg ggatgggttg acatggcagt tcccggcttg gggtcgaagg
     3061 gctgcacaat gacctcttga atatggccat actcatcatg aagtcgagat atagcttgaa
     3121 tggattggat tctgtcctgc cagctctctc ctatgcccac taaaatgcct gtggtaaagg
     3181 ggatatgcag cctgcccgcc ctggcaatat gcttaaggcg cagctccgga tccttgccgg
     3241 gagattgctc atgggcattg agtgtggcac agctctccag catcagcccc atggaggcat
     3301 tatagggctg caaggtggcc aggtcctctt ctgagaggag cccggcgttg gtatgaggca
     3361 gaagatcatg ctctatcgcc agctcgcata gctctaccag gtagcccaaa aggtcctctc
     3421 tgccctctcc tgccagcatc aaatgaaagc cttccacctc ctccggcctc tctcccagag
     3481 tgaatagagc ctcggagcag ccatgatctc gtccctcctc caaaagctgc tgggcagcag
     3541 accggctgat cagatgggcc tcatccaggt ttcttcgaaa agagcagtat tggcagcggt
     3601 tgcggcagag gttggtgacg ggcaaaaaaa tatttttgct gtaagttatc cgcttcatct
     3661 atcggcgaaa ctccaccagc ttcaggatat tcccccgggg gcagtcctcc ggcgcctcac
     3721 ccaccacctc tacaatggtg cacttttctc catccttcag cccgatggga tggcatatat
     3781 cgtacatgct gcaattatag tcatcgcatt tcatggcctc aaatacgatc ttggatccgg
     3841 ggaatgcctt tctggcatcg aaggccgcaa ttattgggct ttctgctacc tccactgccc
     3901 tgactcctgc ctcatgaatg gggcactcat gcaccagctc tccgcgcgtt cccagcaccc
     3961 ggtagcgcct cctcggctcc agattgatac aggtattcct cagcttgcag gattcgcatt
     4021 cgggagtggg gcccatgaag gtgaactcca tgccaatgct ggcaagcttt gttccaatca
     4081 atgtgatttg ggtagatgct tcggacatat ttcattctcc ttttattaat cattattatc
     4141 caattattat atatttcatt gaggtcggtt taccatacta aatcactccc gttgcctcgg
     4201 ccgctctggc ggctgcatcc cggttcagtc ctgagtccag gatggtatag cgctccggcc
     4261 taatcctatg ggcattgacc agggcctcta ccacggtttc tgcatccagc ccgatctggt
     4321 cggcggaggt gggagctccc acctgggcca gtgcctctct tatcatcttc cagtctttgc
     4381 cgtgcaaata catcatcatt atggttccca ggccgcagag ctcaccatgc aggccccttc
     4441 tcggtgctat gcggttgatc gaatgggcga acttatgctc cgagccgctt gccggccggg
     4501 aggatccggc aatgctcatc gccaccccgc tggagatcag agcctttacc acgatcttgg
     4561 ccgattcctc cagccctggc cggatatcag aagcacgctc gacgatcatt ttagcggtca
     4621 tgccggagag ggcggaagca tactcgctga actcctcgct tctcagccgg tgagcaagct
     4681 gccagtccag gatggcggtg tagttggaga ttatgtctcc gcagccggcg gccagaagcc
     4741 tggccggagc ccgagctatt atcccggtgt cagcgacgat ggcgaggggt gattgggcgg
     4801 gcagggacac cgcctctccg ttaaccgtca atgaggcctg ggaggagcag atgccatcgt
     4861 gcgaagcggc ggtgggcagg gagaggaagg gaatattcgt tctcatggag gcgatcttgg
     4921 ctatatcgat ggatctgccc ccaccagcgc cgatgagaaa gccggcctca atctcccttg
     4981 ccctggcctc tgcgcgatcc acctcctcaa gctttgatgc cgtgcagatg agggtttggg
     5041 gctcaaaccc tcctgagttc agcagctcta agatggtctt tcccaccaca tcatcggtat
     5101 gaggcccggt gacgatgagt ccgggcccct ccagatgcag ataagagcag atctggggca
     5161 gctccaatat cgcctcgttt cccacggcca ctatccgcgg cagctccatc catctcgatc
     5221 cagtcaaccg actcacccgc ttagaaatga ggttagaatt agtgctcttt ggcgatataa
     5281 aaccatatct cagcagctgg caatctcagt gcgcaggtcg cagaggatgc gatcccttat
     5341 ctggttgacc tcagtgctgg ttctagtcct gggcctgggg agggggatct gaattatatc
     5401 ctttatcctt cccggccggg cagacatgat cactattgca tccgccagat atatggcctc
     5461 gtcaacgctg tgagtgacga atagtaccgt cttcttctcc tcctcccaaa ttctgagcag
     5521 ctcagactgc atggtatttc gggtctgagc gtccagggct ccaaaaggct catccatgag
     5581 cagggccttg gggtcgttga ccagagctct ggcgatggcc accctctgct tcattccccc
     5641 ggaaagctca tgaggatagc gcttctcaaa tccctccagg ccgaccatct tcagatactg
     5701 tcttcccctc tcctctctct tggccttatc gatgcccttg agctccaggc cgaatacggt
     5761 gttatccagg attgtccgcc agggaaagag ggagtactcc tggaagacca tgcccctctc
     5821 aggcgaaggg cctactatgg gctctccatc tattgtggcg gcgccagagg tgggcgcctc
     5881 caggccggcc actatgcgaa gcagcgttgt ctttccgcat cccgacggtc ctatgaagca
     5941 gacgaattga ttctcttcga tggtcaggtt gatgtca
//
LOCUS       DAOSGP010000059         5977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11707_length_5977_cov_18.293144, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000059 DAOSGP010000000
VERSION     DAOSGP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11707_length_5977_cov_18.293144"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PLV49_02855"
     CDS             complement(<1..1046)
                     /locus_tag="PLV49_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOG97813.1"
                     /translation="MDLEGFAKRGLRRRDPEIESKLIDLIREVKEIPSDRAAILAEAV
                     LLEAEATLDPRGEVFQLNNVGVSMGDFGVGSRGSGDFYTHTKIAEVIGRTEAVVDSRQ
                     LDDSGVVQAGKKYITVTVDGMHSRLSDYPFLAGFHVTRAALRDVYVMGAKPVALLSDV
                     HLADDGDVSKLFDHIAGIATVSELIGVPLITGSTLRIGGDMVIGDRLTGCVGAVGVSD
                     KLTARKSARPGDVIMMTEGAGGGTICSAALYYDRHEVVDETLNIKFLEASEALLKVNA
                     RIHAMTDVTNGGIRGDAKEISYTAGVRLIFEEERMRRLVNSRVLEMLNELKIDYLGVS
                     IDALLIIAPPEEAEA"
     gene            complement(1089..2804)
                     /gene="mutL"
                     /locus_tag="PLV49_02860"
     CDS             complement(1089..2804)
                     /gene="mutL"
                     /locus_tag="PLV49_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG97814.1"
                     /translation="MKRIRALDEETVNKIAAGEVIERPASVVKELVENSIDAGAHKVL
                     IEVRDGGKSFIKVTDDGSGIDPDDLPLAFQKHATSKISGALDLEKIVTLGFRGEALAS
                     IASVSKAVEVRTKTRDALSGSYLRIENGKVAEAKEVGSPAGTSIVVWNLFSNVPARRK
                     HLKGAESELVHIIDVITELAIIHYDIAFELFSGSRTHFKSARSNSWDDVLSRIFGLKA
                     VAGMAPLQASGRGWRIEGMIGDAFNLRASPDRIFIFVNGRAVSSRSMAGALREAYRNI
                     IPPGKSPIAVLSLEISPDLVDVNVHPAKREIRLLHENEICSAITQEAALALSSYAKSV
                     ASERLKPSDQITASEETLAQNALQSTLPLDVEEDTVLDQALPEREKRSSLKILGQIKR
                     LYIVAESDQGLVLIDQHAAAERIRFEGLEERYREGLIRQELACPVTIELMASEEIMLS
                     SWKEVLDDIGFEISSFGGRSYSVRSVPALGQRTESAESVHDVLKELFLRGKPGPDSSR
                     RDEILKLLACRGSIKSGKELTLKEMEQLLHDLQGCSNPSTCPHGRPVMVTLDQSQLER
                     LFGRR"
     gene            complement(2808..5441)
                     /gene="mutS"
                     /locus_tag="PLV49_02865"
     CDS             complement(2808..5441)
                     /gene="mutS"
                     /locus_tag="PLV49_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG97815.1"
                     /translation="MPPAKPSPLMEQYYQNKKLYPDALLLFRVGDFYETFADDAVIVA
                     RDLNITLTSRQKDDQGEKIPLAGVPYHSLDVYLARLIRAGHKVAICDQVEDPKLARGL
                     VKRAITRVVTPGTIIEPSMLDESSNNFLAAIVKGDENVGLAFVDVSTGEFLTTEVPSN
                     RLYSELARFRPAECLSSFSLHWEGTSLQILEEPCFSAERAEAALADRYGPDWKERLRL
                     EGRALSQRACGAVLSYLNASRFDLLGHLKDVQIYSGSDYMVLDEVTVRNLEITRNIRD
                     RSRRGTLLEFLDQTKTAMGARTLARWLQMPLQSEQAIARRLDAVEELAGKSLLHRSLA
                     EELKGTSDLERLLSRISCKSASPKELSVLKSTLEMLPRLQEILMNDQSSAQSSYLQDL
                     SSRLSPLDDIVSLIERSIMEDPPVHVRDGGVIRDGYDPEIDQLRELLRDGRGWISRLE
                     GSEKERTGIKSLKIAFNNVFGYYIEVSRANLHLVPQDYIRKQTLANGERFVTPELKDM
                     EYRVLSAQERSVSLEQELFYQVRDLVASKAGAIQERATALAELDVLISLATSAKENNM
                     IRPEFNQEGRISIRSSRHPVLDKAMRGAFVPNDVLLDTDRNRLIILTGPNMAGKSTFM
                     RQIALTAIMAQTGSFVPAAYASLTLVDQVFTRVGAYDDLSAGQSTFMVEMTEIAHILT
                     SATRKSLVLLDEVGRGTSTFDGLSLAWAISEYLHESIKCKSVFATHYHQLTDLESILS
                     GVRNYSIAVKEDKGAITFLRTVVPGATDKSYGVHVARLAGVPRTVTKRADQILREIEK
                     EALMQPGSGGRSQRRSSRYTQLIFFDGNESGNTAAEAEGEKKDPILEEIESLDLDMMT
                     PREALDRLAQYQRMQREREEG"
     gene            complement(5667..>5977)
                     /locus_tag="PLV49_02870"
     CDS             complement(5667..>5977)
                     /locus_tag="PLV49_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97816.1"
                     /translation="GGSWSFALNDSLQRSMALTLFQKDDQLLGAGKMRVENSTIDAAV
                     SGQVYGNGTARLDIITINPIDLYKLYLNLNGDMASGEFSALSARGESWTGSFEGVKTA
                     "
BASE COUNT         1177 a         1813 c         1617 g         1370 t
ORIGIN      
        1 gcctccgcct cctccggtgg ggcgatgatg agcagggcat cgatggacac cccgagatag
       61 tcgattttca gctcattgag catctctagc actcgggagt tgaccagcct gcgcatcctc
      121 tcctcctcga agatgagcct caccccggca gtataggaga tctccttggc gtcgccccgg
      181 atgcccccgt tggtcacatc ggtcatggca tggatccgag cattaacttt aagcagcgcc
      241 tcgcttgcct ccaggaactt gatgttcagg gtctcatcca ccacctcgtg ccggtcatag
      301 tacagagcag cagagcagat cgttccccct cccgctccct cggtcatcat gatcacatcc
      361 ccgggccggg cgctctttct ggcggtgagc ttatccgaga ctccaaccgc tcccacgcac
      421 ccagttagtc ggtccccgat caccatgtcc ccaccgatgc gcagggtgct cccggtgatg
      481 agaggaacgc cgatcaattc cgagaccgtg gcaatgcctg cgatatggtc gaatagcttg
      541 gagacgtccc catcatcggc cagatgaaca tccgagagaa gtgccaccgg cttggctccc
      601 atgacataga catcgcgcaa ggccgctcgg gttacatgaa atccggccaa aaacggataa
      661 tcgctcagcc gggagtgcat gccgtccacg gtcactgtta tgtacttctt ccctgcctgg
      721 accacacccg agtcgtccag ctggcgggag tcaaccaccg cttctgtgcg gccgatgacc
      781 tcggcgatct tggtgtgggt ataaaagtcg cctgagccac gtgagcccac cccaaagtcg
      841 cccatgctga cccccacatt gttcagctgg aaaacctccc ccctgggatc tagggtcgct
      901 tcggcctcca ataagaccgc ttccgccagg atagcagccc gatcgcttgg gatctccttt
      961 acctctctga tcagatcgat gagcttggac tctatctccg ggtcccgtct cctcaaacct
     1021 cgcttggcaa atccttccag atccatgaga ggtcatttga ccttcccata gatattatca
     1081 tctcctgatt atcttctgcc aaatagcctc tccagctggc tctggtccag ggtgaccatc
     1141 accggcctgc cgtggggaca ggttgaggga ttgctgcatc cctgcagatc atgcagcagc
     1201 tgctccatct cttttagagt gagttccttt cccgatttta tggagccccg gcaggccaaa
     1261 agcttgagga tctcatcccg gcggctcgag tccggccccg gctttccccg caaaaacagc
     1321 tcctttaaga cgtcatgtac cgattctgcg ctctcggtcc tctggccgag ggctggaacg
     1381 gaccgtacgc tgtaggacct ccctccaaag gatgagatct caaagccgat atcatccaag
     1441 acctccttcc aggaggagag catgatctcc tcgctggcca tgagctcgat cgtcaccgga
     1501 caggccagct cctggcggat caatccttcc cggtatcgct cttccagccc ctcaaaacga
     1561 atgcgctctg cagcagcatg ctgatcgatg agaaccagcc cttgatcgct ctcagccaca
     1621 atgtagagcc tcttgatctg gcccaggatc ttcaggcttg atcttttctc cctttccggc
     1681 agggcttggt ccaaaacggt atcctcctct acatccaggg gaagagtact ctgcagagca
     1741 ttctgggcca atgtctcctc cgaggcagtg atctgatcgc tgggcttcaa gcgctcggag
     1801 gctacgctct tggcataaga agagagggct agggcagctt cctgggtgat ggcagagcag
     1861 atctcattct catggagcag ccggatctcc cttttggccg gatggacgtt cacgtccacc
     1921 agatccgggc taatctctaa ggagaggacg gcaatggggc tctttcccgg agggatgatg
     1981 tttctgtagg cctctcgcaa agccccggcc atggaccggg aggagactgc cctgccattg
     2041 acgaagatga atatcctgtc cgggctggcc ctcaggttga aggcatcgcc gatcataccc
     2101 tctattctcc agccccggcc ggatgcctgc agaggggcca ttcctgccac cgctttcaat
     2161 ccgaagatgc gggagaggac gtcgtcccag gaattggatc ttgccgattt gaagtgggtg
     2221 cggctgcctg agaatagctc aaaagcgata tcatagtgga tgatggccag ctcggtgatg
     2281 acatcgatga tatggaccag ctccgattcc gctcccttga ggtgttttct ccttgccggg
     2341 acattagaaa agagattcca gacgacgatc gatgtgcccg caggactgcc cacctccttt
     2401 gcctccgcca cctttccgtt ctctatgcga agatagctgc cagatagagc atccctggtc
     2461 ttggtccgca cctcgacagc tttcgagacg ctggcgatgc tggccaaggc ctcgcctcga
     2521 aatcccaacg tgacgatctt ttccaggtcc agagccccgg agatcttgga ggtggcgtgc
     2581 ttttggaaag ccagggggag gtcgtccgga tctatgccgc tgccatcatc tgtcaccttg
     2641 atgaagctct ttccgccgtc tcggacctcg atcaacacct tatgggcacc ggcatcgatg
     2701 gagttttcca ccagctcctt taccaccgag gcaggcctct cgatcacctc accggcagcg
     2761 atcttattta ccgtctcctc atccagggcc ctgatcctct tcatctctta tccttcctcc
     2821 ctctcccttt gcatccgctg atattgggcg aggcggtcca aagcctccct gggggtcatc
     2881 atgtccaggt ccagtgattc gatctcctct aagatgggat ccttcttctc gccctccgct
     2941 tccgcggccg tattcccgct ctcattgcca tcgaagaata tgagctgggt gtagcgagaa
     3001 gatctcctct gacttctccc gcccgatccc ggttgcataa gggcctcctt ttctatctcc
     3061 ctcaagatct ggtctgccct ttttgtcacc gtcctgggaa cgccggccag gcgggcgaca
     3121 tgcactccat agctcttgtc cgtcgccccc gggaccaccg ttcgcaggaa ggtgattgca
     3181 cccttatcct cctttacggc aatgctgtag tttctcactc cggagaggat gctctccaga
     3241 tcagtgagct gatggtagtg ggtggcgaag acggacttgc atttgatgct ctcatgcagg
     3301 tactcggata tggcccaggc cagggacagg ccgtcgaagg tgcttgtccc tcggcccacc
     3361 tcatccagga gaaccaggct ctttctggtg gcagaagtga ggatgtgggc gatctcagtc
     3421 atctccacca tgaatgtgct ctgtcctgcg gacaggtcgt catatgcccc cacccgtgtg
     3481 aagacctggt ccaccagtgt gagggaggca taggcagcag gcacaaagga cccggtctgg
     3541 gccatgatcg ccgtcagggc tatctggcgc atgaaggtgg atttgcccgc catgttcggg
     3601 ccggtgagga ttatcagacg gttgcgatct gtgtccaata ggacgtcatt gggcacaaag
     3661 gcgcctcgca tcgccttatc cagcaccggg tggcggctgc tgcgaatcga gattcttccc
     3721 tcttgattga actccggccg gatcatattg ttctccttag cggaggtggc cagggagatc
     3781 agcacatcga gctctgccag tgccgtggcc ctctcctgga tggctcccgc ctttgaggcg
     3841 accagatccc gcacctgata aaacagctcc tgctccaaag ataccgatct ctcctgggcg
     3901 gagaggactc gatactccat atccttgagc tccggggtca caaatctctc cccattggcc
     3961 agggtctgct tgcgaatata atcctgggga accagatgca gatttgcccg cgagacctcg
     4021 atatagtagc cgaatacatt gttaaaggcg atcttgagag atttgatgcc cgtcctctcc
     4081 ttctccgagc cctccaggcg gctgatccag cctctgccgt ccctcaagag ctcccggagc
     4141 tggtctatct ccggatcgta gccatccctg atcacccctc catcccgaac atggacgggc
     4201 ggatcctcca ttatcgatct ctctatcaga gagacgatat catccagggg ggagagcctg
     4261 cttgaaagat cctgcagata tgagctttga gccgaggact gatcattcat gaggatctcc
     4321 tgcaggcggg gcagcatctc cagggtgctc ttcaggacgg acagctcttt agggctggcg
     4381 gatttgcatg atatccggct taagagcctc tccaggtcag aggtgccctt gagctcctct
     4441 gccaggctgc ggtgcagcag ggatttgccg gcaagctcct ctaccgcatc cagcctgcgg
     4501 gctattgcct gctcgctctg caggggcatt tgcagccagc gggcaagggt tctcgccccc
     4561 atggcggtct tcgtctggtc caggaactcc agcaaggtgc ccctgcgaga gcggtcgcgg
     4621 atgtttctgg tgatctccag gtttcgcact gtgacctcat ccagaaccat atagtccgat
     4681 cccgagtaga tctgaacatc cttcaagtgc cccagaaggt cgaagcgaga ggcattcaga
     4741 taggagagga ctgctccgca ggccctctgg gatagagctc ttccctccag ccggagtctc
     4801 tctttccagt ccggtccgta gcggtcggcc agggcggcct ctgccctctc cgcggagaag
     4861 cagggctcct ccaggatctg caggcttgtg ccctcccagt ggagggaaaa ggaagacagg
     4921 cactccgccg gccggaaacg ggccagctcg gagtagaggc ggttcgatgg aacctccgtg
     4981 gtcaaaaact cgccggtgga gacgtccaca aaggcaaggc ccacattctc atcccctttc
     5041 actatcgccg ccaggaagtt gttgctggac tcgtccagca tagagggctc gattattgtt
     5101 cccggggtca ccacccgggt gatggctctt ttcaccagac cccgggcgag tttggggtcc
     5161 tccacctggt cgcagatggc caccttatgc cccgcccgga tgagccgggc gaggtaaacg
     5221 tccagagaat gataagggac ccctgccagg gggatcttct ctccctggtc gtctttctgc
     5281 cgggaggtga gggtgatgtt cagatcgcgg gccacgatga ctgcatcatc ggcaaaggtc
     5341 tcgtaaaagt cgcccacccg aaacaggagc agagcatctg gatagagctt cttgttctgg
     5401 tagtactgct ccatcagggg agagggctta gctgggggca ttatgatcta tggttttttc
     5461 gtttttcata tttcatcttc tctgtgcatg ggggacaagc tccttgggga ggacggtagc
     5521 aaaggattca gtctagacgt tgatctatca ctgtctgcca aaaaaggctt tggttgagca
     5581 aagagactgc gaacggaaga ctgaacggat gcgaaaagaa aatagagaga ggatgccgat
     5641 ctttcggcat cgttaccttc acttagttat gcggtcttca ctccctcaaa gcttcccgtc
     5701 caggactctc cgcgggcaga gagggcagaa aactctccgc tggccatatc cccattcagg
     5761 ttgagataga gcttgtagag atctatgggg tttatggtga tgatatccag cctcgcggtt
     5821 ccatttccat agacctgtcc cgatactgct gcatcgatgg tgctgttctc caccctcatc
     5881 ttgcccgcac ccaagagctg atcatctttc tggaagaggg ttaaagccat gctcctctgg
     5941 aggctgtcgt tcagggcaaa tgaccagctt cctccca
//
LOCUS       DAOSGP010000060         5977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11708_length_5977_cov_16.842958, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000060 DAOSGP010000000
VERSION     DAOSGP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11708_length_5977_cov_16.842958"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PLV49_02875"
     CDS             complement(<1..722)
                     /locus_tag="PLV49_02875"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HOG97817.1"
                     /translation="MSKIGKAIRLERILDRKTHRTIIIPMDHGISVGPIAGLIDMPAT
                     VDKVAEGGANAVLGHMGLPLHGHRGYGRDVGLIIHLSASSSLGPDPNHKVLVTDVEDA
                     IRVGADAVSIHVNVGADDEAEMLHDLGRVARSCDQWGIPLIAMMYPRGPKVKSEHNVE
                     HVKLAARIGSELGADIVKTNYTGSPDTFQEVVRGCSVPVVIAGGPKMDTERDLLQMVY
                     DAVQVGGAGVAFGRNVFQAEDPT"
     gene            878..1576
                     /locus_tag="PLV49_02880"
     CDS             878..1576
                     /locus_tag="PLV49_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOG97818.1"
                     /translation="MKWSVVLFCVIIWNALFGAAVGDIYPLTVEGVGTVTVPADIVTI
                     SVSVESSNENITQAQDEVGEDMNRVISALKKAGVKDEEILPGQGSGISSFQSSSRVCK
                     TVNNTTVCENVTESASTLQRSTVIRLQAKDESRIDKVLNAARSAGANAYVAGYYLKDS
                     SDARAEARKKALADARKNAAAMAADAGGSLGKSMDIFAYPDLGIDYSYGGGSGEGRMM
                     DVSSRVMVTYEFIL"
     gene            1613..2314
                     /locus_tag="PLV49_02885"
     CDS             1613..2314
                     /locus_tag="PLV49_02885"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOG97819.1"
                     /translation="MKKICCVLMGMLLLSLVIPAMAADNGTDVSKLRVDGEGKVKAAP
                     DMATIVLGVETRSTSAAEAAEENAILMNQTINALLDAGINESQMQTSRFSLTTMPQDE
                     PRGADATQEPPTFLATNQVTVRLENTADVGRVLDAAVSAGSNSIQEVKFDLKDPTPQN
                     DEAMTIAIKDAQRKAQVAASAAGLELGKILEITVGYGYVMEASRSYSYAMDATPIQPG
                     EMEVTASVNLVYEIS"
     gene            complement(2369..2878)
                     /locus_tag="PLV49_02890"
     CDS             complement(2369..2878)
                     /locus_tag="PLV49_02890"
                     /inference="COORDINATES: protein motif:HMM:NF021571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2299 family protein"
                     /protein_id="HOG97820.1"
                     /translation="MVAPEIADVRDRIKTWLVQEDIFKEEVPSESLYYQIAAEYPARS
                     GRHLSIIQPQGHEDMVVIFSRIMLADVHQKAVMHLLPQQKERLMWQIRYDLLFRQFSF
                     EIEPAGEDMRSIRFTREIYYDGLTKDRLMEAIRENFKCELYVVWKFQELFGEGSAAKD
                     SKPPEPMYI"
     gene            complement(2950..>3844)
                     /gene="fhcD"
                     /locus_tag="PLV49_02895"
     CDS             complement(2950..>3844)
                     /gene="fhcD"
                     /locus_tag="PLV49_02895"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: protein motif:HMM:NF002554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HOG97821.1"
                     /translation="FMEINGVQIDDTFAEAFPIKMARVQITAVTERWALEAAREATGF
                     GTSVIGCPAEAGIECLVDGRETVDGRPGVNILLCNMGFKNLENSLLMRLGQCVLTAPT
                     AAAYSGMHDAEKQFDTGKKLSFFGDGYQKTEEMFGRNIWKIPLMSGDFIIENSFGAVE
                     GIAGGNFLILGQNQMSGLMAAEAAIDSIRKVKGAITPFPGGVVSSGSKVGSKYKFLKA
                     STNSAFCTSLREDGVCVLAEDVSCVFEIVINGVDREAIENAMRAGIHAACQVPGILKI
                     TAANYEGKLGIHHFPLHSVLE"
     assembly_gap    3845..3944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3945..4362)
                     /locus_tag="PLV49_02900"
     CDS             complement(<3945..4362)
                     /locus_tag="PLV49_02900"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOG97822.1"
                     /translation="MYVGRIVVAGRAQGRSFVAYRVSSRSFPNRRAEVHEKSITVMPL
                     DSADLARNPYISYNCIRVADDVAVVTNGTHTDMITERIEDGQSPGDAMALSLLAYGHE
                     RDELDTPRIAGAVRGNRVWLGIARKDELRVQQFRLDE"
     gene            complement(4365..5240)
                     /locus_tag="PLV49_02905"
     CDS             complement(4365..5240)
                     /locus_tag="PLV49_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG97823.1"
                     /translation="MERSLLAPGHRACAGCAMPTTIRLVLDAAGPNTIVVSPTGCLEV
                     VTTPFPESAWCVPWIHSLFENAAAVASGVEVMLKRQGRDTNVVVIGGDGSTFDIGMGS
                     NSGMFERGHKVLYVCYDNEAYMNTGVQRSGSTPFCVHTTTTPSGSESFGNPRPKKDMP
                     GIALAHGVPYVATASVAYPVDLRRKVKEALKANGPSYIQVNAPCITGWTFDAGTMIEL
                     ARLAVETGLWPLTEYVDGKLVGVKKIRRPKPVEEYLRLQGRYKHLFKKPNGADMLKHI
                     KALADGNIARYNLVD"
     gene            complement(5253..>5977)
                     /locus_tag="PLV49_02910"
     CDS             complement(5253..>5977)
                     /locus_tag="PLV49_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOG97824.1"
                     /translation="AEDPEIRTPIMVCMDGFILTHVYEPVELLDKEKAREFLPDFKPA
                     NILDPNNPLTFGAFADPSTFTEFRYQQFAAHQKALEKIEQVARDFERAFGRYYGGLVD
                     SYCVEGAEVVIVTMGSIIGTIKDAIDEMRAEGIKVGLVKVRCYRPFPAAALRKALSGA
                     KVIAVVEKDVSIGNEAGLVTDLKASFYNSDIRTPIIGFVAGLGGRDITIKDIRKMVEK
                     ALAAEKGIECEFEFLDLRKEIL"
BASE COUNT         1319 a         1673 c         1560 g         1325 t
ORIGIN      
        1 gttggatcct ctgcctggaa gacattgcgg ccgaaggcca cccctgctcc gcccacctga
       61 accgcatcgt agaccatctg caatagatcc cgttcagtgt ccatcttcgg tccgccggcg
      121 atgacaacag gcacgctgca gcctcttacc acctcctgga aggtgtcggg cgagccggtg
      181 taattcgtct tgactatatc cgcccccaac tccgagccta tgcgggcggc cagcttcaca
      241 tgttctacat tatgctcaga cttcaccttt ggcccgcgtg gatacatcat ggcgatcagg
      301 ggtatgcccc attggtcgca gcttcgtgcc acccttccca ggtcatggag catctccgcc
      361 tcatcgtcgg cgccgacgtt aacgtggatg ctcacggcat ccgctcccac tcttatggcg
      421 tcctcgacgt ccgtcaccaa aaccttgtga tttggatcgg gacctagtga gctggatgca
      481 gagagatgaa tgatcaatcc cacatccctt ccatatcccc ggtggccatg caatggcagg
      541 cccatatgtc ccaggacggc gttggccccg ccttctgcca ctttgtctac cgtggccggc
      601 atatctatca gtccggcaat gggtcctaca gatattccat ggtccatggg aatgatgatg
      661 gtcctgtgcg tcttgcgatc caggatgcgc tccagcctga tcgcctttcc gatcttgctc
      721 atgaaggtgg tggatgttta ttctgaaatt aaagatggtg gtcgaatgaa gaagaagaga
      781 acggaatgac aggaggggat ggaacgggtc tcttatgggc aacgagacca gcagagcgct
      841 taaatatctt cgagtatttc catagatggg gattaatatg aagtggtcgg ttgtcttatt
      901 ctgcgttata atatggaacg cgctctttgg agctgcagtg ggtgacatct accctctgac
      961 ggttgagggg gtgggaacgg tgaccgttcc tgcagatata gtcacgatct ccgtctcggt
     1021 tgaaagcagc aatgaaaata ttactcaagc ccaagatgag gtcggagagg atatgaaccg
     1081 ggtgatatcc gccttgaaga aagcgggggt caaggacgag gagatcctgc ccggccaggg
     1141 aagcggcata tccagcttcc agtcctcgag cagagtctgc aagacggtga ataacaccac
     1201 tgtatgcgag aatgtcacag agagcgcatc cactctgcaa aggtccacgg tcatccgtct
     1261 ccaggccaaa gatgagtcca ggatcgataa ggtgctgaat gccgctcgat cggcaggggc
     1321 taacgcctat gtggcgggat actacctgaa ggactcaagc gacgcccggg cagaagcccg
     1381 caagaaagct ctggccgatg ccagaaagaa tgcagctgcc atggccgcgg atgctggggg
     1441 aagcctggga aagtccatgg atatcttcgc ttatcccgat ctgggaatag actattccta
     1501 tggcggtggc tcgggcgaag gcaggatgat ggatgtcagc tccagggtca tggtgaccta
     1561 tgagtttatc ttataagaca cttcaatgac atcagtataa aggggttagt ctatgaaaaa
     1621 gatatgctgt gtattaatgg ggatgctgct attgtcattg gtcatacctg caatggcggc
     1681 agataatgga acagatgtat ctaagctgag agtggatggc gagggcaagg tgaaagctgc
     1741 cccggatatg gcaacgatag ttcttggggt tgagacccgg agcacaagcg cagcagaagc
     1801 agccgaagag aatgccatcc tgatgaacca aaccatcaat gccctgctcg atgcgggcat
     1861 caatgagagc cagatgcaga ccagcaggtt cagcctgacc acaatgcctc aggacgagcc
     1921 ccggggcgct gatgcaacgc aagagcctcc gaccttcctg gccacgaacc aggtaacagt
     1981 gaggctggag aatactgcgg acgtgggccg ggtcttggat gcggcagtga gcgcgggctc
     2041 aaacagcatc caggaagtca agttcgacct caaggatcca acccctcaga atgatgaggc
     2101 catgactatt gccatcaagg atgcgcaaag gaaggctcag gttgccgcca gcgccgccgg
     2161 attggagctg ggaaagattc tggagataac agttggctat ggatatgtca tggaggcttc
     2221 cagaagctat tcatatgcaa tggatgctac tccgatccag ccaggagaga tggaagtgac
     2281 ggccagcgtg aatctggttt atgagatctc ttgagatgtc ctcaagaggc ctcatgcctc
     2341 tattttctat ccaccagatc catatcggct agatatacat cggttcaggc ggctttgagt
     2401 cctttgctgc tgatccctcg ccaaacagct cctggaattt ccagaccacg taaagctcac
     2461 acttgaagtt ctctcttatc gcttccatca gcctgtcctt ggtcaggccg tcgtagtaga
     2521 tctcccgggt gaaacgaatg ctccgcatat cctctcctgc aggctcgatc tcgaagctga
     2581 actggcggaa caggaggtcg tagcgtatct gccacatgag cctctctttc tgctgaggca
     2641 aaaggtgcat gacagccttt tgatggacat cagccagcat tatccggctg aagatcacaa
     2701 ccatatcctc gtggccctgg ggctgaatga tgctcaaatg cctgcccgac cgggcaggat
     2761 attcagcagc gatctgatag tataggctct cgctgggcac ctcttccttg aatatgtcct
     2821 cctgtacaag ccaggtctta attcgatccc gaacatcggc tatttctggg gcgaccatca
     2881 cgagctatca actcctatga ttcaaatatt ttaataaaaa ctaaaaagag aaaatgtctg
     2941 agccttgttc tactccagca cgctatgcag cgggaaatga tgaatgccca gcttcccctc
     3001 gtaattggct gcggttatct tcagaatgcc aggcacttga caggcagcat ggattcccgc
     3061 ccgcatggca ttctctatgg cctcccggtc aacgccattg atcactatct caaagacgca
     3121 agagacatcc tccgctagaa cgcagacgcc gtcctcccgg agcgatgtgc agaatgcgga
     3181 gttggtggag gccttgagga acttgtactt tgagcccacc ttcgatccgc tggagacgac
     3241 acctcccggg aagggggtga tagcgccctt gaccttgcga atggagtcta tcgccgcctc
     3301 ggctgccatc aggccggaca tctggttctg tcccagtatc aggaaattgc ctccagcaat
     3361 tccttcgacc gctccaaagc tgttctcgat gatgaagtcg ccggacatga gaggtatctt
     3421 ccagatgttc cgtccaaaca tctcctctgt cttctggtag ccatccccga agaagctcag
     3481 cttcttgccg gtatcaaact gcttttccgc atcatgcatg ccgctgtaag ccgcggcagt
     3541 gggtgcagtg agcacgcact ggcccagcct catgagcaat gagttctcca ggttcttgaa
     3601 gcccatgttg cataagagga tgttcacacc aggccggccg tcgacggtct ctcttccatc
     3661 cacgaggcac tctatgcccg cctcagcagg acatccaatc actgaggttc caaatcccgt
     3721 ggcttccctc gccgcctcca gggcccacct ctctgtcact gctgtgatct gcacccttgc
     3781 catcttaatg ggaaaagctt cagcaaaggt atcatcaatt tgcacaccat tgatctccat
     3841 aaacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctcat ccaacctgaa
     3961 ctgctgcacc cggagctcgt ccttcctggc gatccccagc cataccctgt tcccgcgcac
     4021 agccccagcg atgcggggtg tgtccagttc atccctctcg tggccataag cgagcaggct
     4081 caaggccatg gcatctcccg gactctgccc gtcctcgatc ctctctgtga tcatatcggt
     4141 atgagtacca ttggtgacca ctgccacatc atctgccacc cgaatgcagt tgtaggagat
     4201 gtaagggttc cgcgccaggt ccgcagaatc caaaggcata actgttatcg atttctcatg
     4261 gacctctgcc ctccggttgg gaaaagagcg ggaggagact ctgtaggcga caaagctcct
     4321 tccctgggca cggcctgcga ccacgatccg gcctacatac atcttcaatc aaccagatta
     4381 tacctggcga tgtttccatc tgccagtgct ttgatatgct tcaacatatc tgcgccattg
     4441 ggcttcttga agagatgctt gtagcggccc tgcaggcgga gatactcctc gacaggtttg
     4501 ggtcggcgga tcttcttcac acccaccagc tttccatcga catattcagt gaggggccac
     4561 aagcccgtct ccactgccag ccgcgccagc tcgatcatgg ttccggcatc gaatgtccag
     4621 ccggtgatgc aaggagcatt gacctggata taggatggtc cattggcctt cagagcctcc
     4681 ttgaccttcc ttctcaggtc cacgggatag gccaccgagg cggtagccac atagggcaca
     4741 ccatgagcca gggcgatgcc tggcatatcc ttcttgggcc tgggattgcc aaaggactcg
     4801 gaacctgatg gggtggtggt tgtgtgaacg cagaagggcg tagagccgct gcgctgcact
     4861 ccagtgttca tataagcctc gttatcgtag cagacataaa gcaccttatg gccgcgctcg
     4921 aacatgccgg agttcgagcc catgccgata tcgaatgtgc tcccgtctcc tcctatcacc
     4981 acaacattgg tatcccggcc ctggcgcttg agcatgacct ccactccgga ggctacagct
     5041 gcggcattct cgaacaggga gtgaatccag ggaacgcacc aggcgctctc cgggaagggg
     5101 gtggttacaa cctccaggca gccggtgggc gagaccacaa tggtgttcgg cccggccgca
     5161 tccaagacca gcctgatggt ggtgggcatg gcgcatcccg cacaggccct gtggcccggt
     5221 gccagcaaag atctttccat ttttatagcc tcctaaagaa tctcctttct cagatccagg
     5281 aactcgaact cgcactcgat tcccttctca gcggcaaggg ccttctctac catctttctg
     5341 atgtctttga tggtgatgtc ccggccaccc agaccggcga caaagccgat aatgggcgtc
     5401 cttatgtcgc tgttgtagaa cgacgccttg agatcagtca ccaggccggc ctcgtttcct
     5461 atggagacat ccttctccac cactgcgatc accttggcgc cagagagggc cttgcgcagg
     5521 gctgccgctg gaaatggccg atagcatctg accttgacca ggcccacctt tatcccctct
     5581 gccctcatct catcgatggc atccttgatg gtgccgataa ttgagcccat ggtaacaatg
     5641 accacctctg caccctcgac gcaatagctg tcaacgagac ctccataata tctgccaaag
     5701 gccctctcaa aatccctggc cacctgctct atcttctcca gggccttttg atgagccgca
     5761 aactgctgat agcggaactc tgtgaaggtg gagggatctg cgaaggctcc gaaggtcagg
     5821 ggattgttcg ggtccagaat gttcgccggc ttgaaatcgg gcagaaactc ccttgccttc
     5881 tccttgtcca ggagctccac cggctcatag acatgggtga gaatgaaccc gtccatgcag
     5941 accatgatcg gagttcggat ctccggatct tcggcga
//
LOCUS       DAOSGP010000061         5965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_11733_length_5965_cov_16.927242, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000061 DAOSGP010000000
VERSION     DAOSGP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11733_length_5965_cov_16.927242"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PLV49_02915"
     CDS             complement(<1..839)
                     /locus_tag="PLV49_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HOG97825.1"
                     /translation="MLEPVMFTGGLYKHDLVLELVEDLGGYILQKNVTQTEIILLLLV
                     PSEDMNALEILSRDLRGELVRAPLAGTEVAVVTPTLAIHHLPHVACDIAEYLRRHGSK
                     TNMIGMARGVGREIAQINEYETALINEHDAAVFIFGNFENCIKKKEDLYRNISVPVII
                     TGGPKIDPEELPYAFGYVPSIGRMAHRTRKATEIGSLDNIIEMVGRALDTTREAINKD
                     PLTTSPPRVMDAVREQVPEVEFSYSPLPIALNLTGVRVKLPYGGYRDKVAAVTFDEGV
                     KLG"
     gene            complement(832..1404)
                     /locus_tag="PLV49_02920"
     CDS             complement(832..1404)
                     /locus_tag="PLV49_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HOG97826.1"
                     /translation="MDPMEVFKIEVTGGEEYGAKKYREIIMDILQDLGLIRSIGRLYV
                     YVDISVPVFAVYGLLRSGIPPLTVKDVGDVMKVGGGHQIKINDEEHMADLLKALWKRY
                     GRERVEQPARDIVIIASDSSPADLMVTDMEAEFLQDLTDALVRVVPEGFRNRRNIMTK
                     DSFFFVAAEESIKPELISEIEEKIREMENA"
     gene            complement(1406..>2239)
                     /locus_tag="PLV49_02925"
     CDS             complement(1406..>2239)
                     /locus_tag="PLV49_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HOG97827.1"
                     /translation="VKYRGLLAREKQKGLTAGIDSGSTTTKSVVMRDNKVIGTGWVPT
                     TEVLKSAEDAFQEALKAAGVNKEDIQAVGVTGYGRFLVGKHINAKLIQEEITVNSKGA
                     VFLADAQKGPATVIDIGGMDNKAISVQDGIPGGFTMGGICAGASGRFLELAARRLGVD
                     ITELGKLALKGDYRKVAMNSYCIVFGTQSLVNSLSMGNKPEDVAMAACHSVAEQVYEQ
                     QLQEVEVKEPVIMVGGTSLIEGLPKAMEELLQVKVTVPPYAQYIGAVGAALLVSGLLE
                     G"
     assembly_gap    2240..2339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2497..2934)
                     /locus_tag="PLV49_02930"
     CDS             complement(2497..2934)
                     /locus_tag="PLV49_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HOG97828.1"
                     /translation="MAKVIVYPPTSMILSDLVDRMGHKALVVPEVVRKLATDNNLASP
                     PLNVTSEEPKKGLRYAAVDVPSGIRGRMALIGPIIEEAEAAIIVQDPGWLTGCAGCNR
                     TNELVRLLIRTKGIPILDLSYPTNDAEARDFVQKIKNFLGDLK"
     gene            complement(2950..3363)
                     /locus_tag="PLV49_02935"
     CDS             complement(2950..3363)
                     /locus_tag="PLV49_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HOG97829.1"
                     /translation="MLVTKYLIMSSESNILPSDIAMKIYGSGYDVLVKETCFGVIING
                     EEEVIEKLLKEIRSLDPAGIFVKDRGFPPGDPRRCRGRRGGGARPGFYMIESESKLLP
                     MISRALASEEECEPVPEKKARPIPLERLEEIAKED"
     gene            complement(3363..>3664)
                     /locus_tag="PLV49_02940"
     CDS             complement(3363..>3664)
                     /locus_tag="PLV49_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HOG97830.1"
                     /translation="PVYFTYENTLLFKPEVEAVSYKELLPENKPTGPVPAGSIGISNQ
                     VSKKIGLVGIRLEEDKRYGPSGEKFEATNIIGRVLEPDKLRDVKEGETIYIREER"
     assembly_gap    3665..3764
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3765..4705)
                     /locus_tag="PLV49_02945"
     CDS             complement(<3765..4705)
                     /locus_tag="PLV49_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HOG97831.1"
                     /translation="MRVFVDGKEIELAAGACLADALQKAGSNPAKGAIVGVVKGRGEK
                     SRQTNSYWLNTTKGKIRIELLENELQKIWHEVVEKISGSQVRWASADGIAFGPFSSRI
                     SFARDAHEYNRWEVVLGAAGFDADNTQLIFMQRRHSAVYGTPKDGGVFAHVVGGKNTL
                     DRLEIGDRIESIEPIVEWQDLTEKTSTLDLTLPVEEGMEIYTRVQAELIEDAPLGAEF
                     FLALTRDGTFRVDSVSSSYISSDHLLTEHVAYEHREPRLEGVVTVRTTGRGLGRIFIY
                     KHDRTSNPSHSVVARVIKGMDMVKLAKPGQMITFDVTP"
     gene            complement(4707..>5965)
                     /gene="atwA"
                     /locus_tag="PLV49_02950"
     CDS             complement(4707..>5965)
                     /gene="atwA"
                     /locus_tag="PLV49_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HOG97832.1"
                     /translation="IAIMLQRTFALYGDERVIVNVMRAVEETGDTMSVHVAADLLDEV
                     NLSHRLMHVARELSGGEKQRVVLARQLAKSPMILLADEPTGTLDPATARVVHDSIKRA
                     VNDFKMTLIITSHWEDVMVELTQRAILLDQGEIKAEGKPKDVAAQFLAMAGTLAKREE
                     VELGEPMIKANGLSKRYISIDRGVVKAVDDVSLEVNEGEIFGLVGISGGGKTTLSKMI
                     SGILTPTGGSISVRVGDEWVDMTVLGSNGRGRATKYIGILHQEYTLYPSRSVIENLTE
                     AIGLSLPFELAERKAIHTLETVGFSEEQAEAVLEKMPHELSEGERHRVAMAQVLIKEP
                     RIVVLDEPTGTMDAITKIEVTNSILNAREELGETFIIVSHDMDFVKNVCDRAMLMRNG
                     RAIFTGLPDEVLNKITEEEEKEMLGS"
BASE COUNT         1185 a         1770 c         1403 g         1407 t
ORIGIN      
        1 tcgcccagct tcacaccctc atcgaaggtc actgctgcca ccttatcccg atagcctcca
       61 taaggaagct tcacccgcac cccggttaga ttgagggcaa ttggcagagg ggaataggag
      121 aactcgacct ctgggacctg ctctcgcacc gcatccatca ccctgggcgg agaggtggtc
      181 agaggatcct tgttgatcgc ttctcgggtt gtgtccagcg cccgtcccac catctcgatg
      241 atattgtcca gggatccaat ctccgtggcc ttgcgggtcc tgtgggccat ccggccgatg
      301 gacggcacat agccgaaggc atagggcaac tcttctggat ctatcttcgg cccaccggtg
      361 attatgacag gcacgctgat attccggtag aggtcctctt tcttcttgat gcagttctcg
      421 aagttgccga agatgaagac tgcggcatca tgctcgttta tcagagcggt ctcatactca
      481 tttatctggg cgatctccct gcccacaccc cgagccatgc caatcatatt ggtcttggag
      541 ccatgcctgc gcagatactc ggcaatatcg caagccacat ggggcagatg atgaatggcc
      601 agggtgggtg tgaccaccgc cacctctgtt cccgccagcg gcgcccttac cagctctcct
      661 ctgagatctc ggctcaggat ctccagagca ttcatgtcct ctgaaggaac cagcaataaa
      721 aggatgatct ccgtctgagt aacattcttc tgcaggatgt agccgcccag gtcttccacc
      781 agctccagta ccaggtcgtg cttgtacaac cctccggtga acatcacagg ctcaagcatt
      841 ctccatctcc cggatcttct cttcgatctc tgatatcagc tccggcttga tcgactcctc
      901 ggcagcgacg aaaaagaagc tgtcttttgt catgatgttt cgccggttgc gaaatccttc
      961 cggcactact ctgaccaggg catcggtcag atcctgcaga aactccgcct ccatatccgt
     1021 aaccatcaga tcggcaggcg aggaatctga ggctatgatc acaatgtccc ttgccggttg
     1081 ttccaccctc tcccgcccgt atcgcttcca caaagcctta aggaggtctg ccatatgctc
     1141 ttcatcattg atcttgatct ggtggccgcc tccgaccttc atcacatctc ccacatcctt
     1201 aaccgtaagg ggcggtatgc cggaacggag gaggccgtag actgcgaaga ctggaactga
     1261 aatgtcgaca tagacataaa gcctgccaat ggacctgatt agacccaggt cctgcaggat
     1321 gtccattatt atctctcggt acttcttggc tccgtactcc tccccgccgg tcacctctat
     1381 cttgaagacc tccattgggt ccatctcagc cctccagcag ccccgatacc aggagagctg
     1441 cgcctacagc cccgatatat tgggcatagg ggggaacggt caccttgacc tgcagtagct
     1501 cctccatcgc tttgggcagg ccttcgatga gcgaggttcc gcctaccatg atcaccggct
     1561 ctttaacctc tacctcctgc agctgctgct catatacctg ctcagcaacg gagtggcagg
     1621 cagccatcgc cacatcctcg ggcttatttc ccatggaaag gctgttgacc aggctctgag
     1681 tgccgaagac tatgcagtag ctgttcatgg ccactttcct gtagtctccc ttcagggcca
     1741 gtttgcccaa ttcggtgatg tcgactccca ggcggcgggc ggcgagctcc aggaaccggc
     1801 cggatgcacc ggcgcatatt ccgcccatgg taaagccccc gggaattccg tcctggaccg
     1861 atatggcctt gttgtccatg ccgccgatat cgataactgt tgccggcccc ttctgagcat
     1921 ctgccaggaa gactgctccc ttggagttaa cggtgatctc ctcctggatg agcttggcat
     1981 tgatatgctt gcccaccaga aatcgaccgt acccagtaac gcccactgct tggatatcct
     2041 ctttattgac ccctgccgcc ttgagagcct cctgaaaagc atcctctgcg ctcttcaaga
     2101 cctctgtggt gggaacccag ccagtgccta tcaccttatt gtctctcatc accactgact
     2161 tggtggtggt ggatccagag tcgattcccg cggtgaggcc cttctgcttc tcccgggcca
     2221 ggaggcctct gtacttgacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     2341 aagccactcg taccccgaac ttctcttcca cattgagcat gtcatctaaa gagacctccg
     2401 ggtagactat cttggcccct acggtctctg ccgccctttc gatctctttt tgaatgccgc
     2461 tgtactctgc gccacaggag agctgtgcaa tgcgaatcac tttaaatccc ccaagaaatt
     2521 cttgatcttc tgcacgaaat ccctggcctc ggcatcattg gtggggtaag aaaggtccaa
     2581 aatgggaatt cctttggtcc tgatcagcag cctgaccagc tcattggtcc tgttgcatcc
     2641 cgcgcatcct gtgagccacc cggggtcctg gacgatgatc gcagcctcgg cttcctcgat
     2701 tatcgggcca atcagggcca ttctgccccg gattcctgag gggacatcga ctgcagcata
     2761 gcgaagccct ttcttgggct cctcagaagt gacattcaaa ggcggtgagg cgagattatt
     2821 atcggttgcg agctttctca ccacttcggg aactacaagc gccttgtggc ccatcctgtc
     2881 cacaaggtcc gagaggatca tgctggtggg aggataaacg attacttttg ccattgattt
     2941 acctcagaat taatcttctt ttgcgatctc ttccagcctc tcaagaggaa tgggacgcgc
     3001 cttcttttcc ggaacaggct cacactcctc ctccgaggct aaagccctgg agatcatagg
     3061 cagcagcttt gactcagact cgatcatgta gaagcctggc ctggctcctc cgcctctgcg
     3121 accgcggcag cgccttgggt cgcccggtgg aaatccccga tctttgacga atatgccggc
     3181 cggatccaga ctgcgaatct ccttaagaag cttctctata acctcctctt ccccgttgat
     3241 aataacgccg aaacaggtct ccttgaccag gacatcgtag cctgaaccat agatcttcat
     3301 ggcgatatcc gaagggagaa tattggactc agaggacatt attagatact tagtgaccaa
     3361 cattaccgtt cctcccgaat gtagatcgtc tctccttcct tcacatctct gagcttatcc
     3421 ggctctaaaa cccgaccgat gatgttcgta gcttcgaact tctcgcccga gggcccatat
     3481 cgtttgtcct cttccagacg gatgcccacc agaccgatct tctttgagac ctggttagag
     3541 atgcctatcg agccggcagg cacaggccca gttggcttgt tctcaggaag tagctccttg
     3601 tagctcaccg cctcgacctc cggcttgaag agcagggtgt tctcataggt gaagtagaca
     3661 ggcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggggtt acatcaaatg
     3781 ttatcatctg gccgggcttt gccagtttga ccatgtccat gcctttgatt accctcgcca
     3841 ctacagaatg gctgggattc gaggtgcgat catgtttgta gatgaagatc ctgcccagac
     3901 cccgtccggt cgttctgacc gtgaccacgc cctccagacg gggctcacga tgctcatatg
     3961 caacgtgctc cgtcagaaga tggtcggatg agatataaga gctggatacg gaatcaaccc
     4021 tgaaagtgcc gtctctggtc aaagcgagga agaactccgc accaagtggc gcgtcctcga
     4081 tgagctctgc ttggacccga gtatagatct ccatgccctc ctccacgggt agagttagat
     4141 ccagggtgga cgtcttctca gtcaggtcct gccattccac aatcggctct atagactcaa
     4201 ttctgtcccc gatctccagc cggtcaaggg tgttcttgcc tcctacgaca tgagcaaaca
     4261 cccctccatc cttgggcgtg ccgtaaacag ctgaatgcct cctttgcatg aatatcagct
     4321 gagtgttatc tgcatcaaag ccagccgcgc ctaagaccac ctcccagcga ttgtactcat
     4381 gcgcatccct ggcaaaagat attctggatg agaacggccc aaaggctatg ccatctgctg
     4441 aagcccatct cacctgagag cctgagatct tttccacgac ctcatgccag atcttctgca
     4501 gctcattttc taaaagctca attctgattt tgcctttggt ggtattgagc caataagagt
     4561 tggtctgacg cgatttctcg ccccggcctt tgaccacccc cacgatagcg cctttagcgg
     4621 gattgctgcc tgctttttgc agggcatccg ccagacaagc tccagctgcc agctcaatct
     4681 ctttgccgtc gacgaagact ctcaaattaa ctccccagca tctccttctc ttcctcttct
     4741 gtgatcttgt ttaagacctc gtccggcagg ccggtgaata tagcccttcc attgcgcatg
     4801 agcatggccc gatcacatac gttcttcacg aaatccatgt catggctcac gataatgaac
     4861 gtctcgccca gctcctcgcg ggcattgagg atggaattcg tgacttcgat cttggtgatg
     4921 gcatccatcg ttcccgtggg ctcatccagg accactatcc tcggctcttt tatcaagacc
     4981 tgcgccatgg caaccctgtg cctctcgccc tcggagagct catggggcat cttctccagc
     5041 actgcttctg cctgctcttc tgaaaaccca accgtctcca gggtatgtat agcttttctc
     5101 tcagccagct cgaagggaag agagaggcct atggcttccg tcaggttctc gataacgctg
     5161 cgagagggat agagggtata ttcctgatgg aggatgccga tgtatttggt ggcgcgacct
     5221 cgaccgttgg aaccgaggac ggtcatatcc acccattcgt ctcccacccg cacagagatg
     5281 ctgccgccgg tgggagtgag aatgccgctg atcatcttgg agagagtggt cttgcctccc
     5341 ccagagatgc ccaccagacc gaagatctcg ccctcattga cctccaggct gacatcatcc
     5401 actgccttta ccacgccccg gtctatgctg atgtatctct tcgaaaggcc attggccttg
     5461 atcatcggct cgcccagctc gacctcttcc cgcttggcca gagttccagc catggccagg
     5521 aactgagcgg ctacatcctt tggcttacct tctgccttga tctctccctg atcaagcaaa
     5581 atagccctct gggtcagctc gaccatgaca tcttcccagt ggctggtgat gatcagggtc
     5641 atcttgaagt cgttgaccgc ccgcttgatg gaatcgtgaa ccaccctggc tgtggccggg
     5701 tcgagagttc ccgttggctc gtctgccagc aggatcatgg gagatttggc cagctggcgg
     5761 gccaggacaa cgcgctgctt ctctcctcca gacagctctc gggcgacatg catcagccgg
     5821 tgagatagat tcacctcatc caacaggtct gcagctacat ggacgctcat agtgtctccc
     5881 gtctcctcca ccgcgcgcat cacattcacg atcacccgct cgtctccata aagtgcaaag
     5941 gtcctctgca acattatggc aatcc
//
LOCUS       DAOSGP010000062         5839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_12086_length_5839_cov_13.320539, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000062 DAOSGP010000000
VERSION     DAOSGP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12086_length_5839_cov_13.320539"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLV49_02955"
     CDS             complement(<1..397)
                     /locus_tag="PLV49_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG97833.1"
                     /translation="MTFEKGSDLVFEKHPNRALSILIAEDDPANQLVALRMLKTLGYN
                     ADTVDNGLEALIAMESRSYNLVLMDIQMPEMDGLQAARIIRKRWPEGPRIIFVTGCTS
                     RRDECISEGGDGFISKPISLHQLQEAIDDK"
     gene            complement(559..1437)
                     /locus_tag="PLV49_02960"
     CDS             complement(559..1437)
                     /locus_tag="PLV49_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOG97834.1"
                     /translation="MRIQSSNLAILMLAIFLIVPAGAETAQSTLKIVCTTSVLMDPAT
                     YIGGDRVEAISIADPTLCPHLQTDIIPNRIQLNMDFIKDANMFMAYNDSNDQRYNMPE
                     INDFMTANNYGTAEWMTISNPSRGWNTPTNSKLLAAEVEGWLANKDPANKTYYEQRYN
                     DYAKSFDAIEPTESERKQLNQTQVIVMLWQKDPVQNWLGMNVVNFFAPEFALNGTKTA
                     ARVVDDINANPEKYQNVKYIIENMQSGELAKGIEESLKDKGIDAERVIFTNFPGSVEG
                     ADTMAEVLNHNKQIVL"
     gene            complement(1521..>1972)
                     /locus_tag="PLV49_02965"
     CDS             complement(1521..>1972)
                     /locus_tag="PLV49_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOG97835.1"
                     /translation="AKQDGKHLKENENIACETYKAVGEIKTLGRYVVMRRCDDFQEDP
                     GVKCIACFASGEQIRDLCALAHFGSHNVFNLISVPWGPACATLVTYPAGMAGNAFLSQ
                     ISIGPTDPSARGWLPENCLAMGIPAEIARKMANDIVRSFLAKIDNVS"
     assembly_gap    1973..2072
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2236..3066)
                     /locus_tag="PLV49_02970"
     CDS             complement(2236..3066)
                     /locus_tag="PLV49_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOG97836.1"
                     /translation="MLEWLIENNVVCHALEAMIFASVACSILGVIISRMSLSSIGFTM
                     SHAAFAGAALGMFLGANAQMAAIAFSMGVAALIGPVSEKAKMAADATLSVLFSLSMAV
                     AIFLISYMEYMGKGLSASSLLFGDVISLYREEIYALAAICCLSILFVLAFYKEISAIM
                     FNMKIAESAGVRVKLVYYVLLFIIALSVALSLNIVGGLLIFVLLVTPASIAGQFCFNV
                     KSMFIVAPLVALLVSAFGVWAGFEFTLPVAPLVALLLTGVFAISVMLSFKRRINTKIH
                     "
     assembly_gap    3180..3279
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3280..3521)
                     /locus_tag="PLV49_02975"
     CDS             complement(<3280..3521)
                     /locus_tag="PLV49_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG97837.1"
                     /translation="MSVIVLESIYTAYEGGSEPVIKDLSLEVDRGEYVVIGGPNSAGK
                     TTLLESINGLVKITHGRACVCGLDVRRRGFEVRRRVG"
     gene            complement(3528..3989)
                     /locus_tag="PLV49_02980"
     CDS             complement(3528..3989)
                     /locus_tag="PLV49_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HOG97838.1"
                     /translation="MDHQEGKCIYTGIDKDYTIEDLAAFHGHLGPYIVLGYMIGRYAR
                     KNLCSDPFQMKAEVFCQDKPPQSCLADGVQIGSGCTLGKRNIRIVPSNEIRCHFAAGE
                     RELVIIPRPIAFPSESGKDHDLLIEQLAEKMYRMVDADLFTADLRRDWDHG"
     gene            complement(4083..4808)
                     /locus_tag="PLV49_02985"
     CDS             complement(4083..4808)
                     /locus_tag="PLV49_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOG97839.1"
                     /translation="MRSTLIDVQSLSKQYGRQKVIDEVSFSVEEGETLGLFGISGSGK
                     TTIGRCIMLLEKPDCGKILFKGRDLLKMNRREVFGVRPRLQMIFQHPETSLNPKMRVK
                     ESIAEPLLIHRRLGRSEIDEELEQLIRQVGLRPEHLERYPQQISGGEIQRAVLARIMS
                     LEPKFVVADEPTSMLDASVQAQILRLMMGLQEDTGLAYLFISHDLDVLRAVSDRIAFI
                     EEGSISLIERNECKKRWSRSKRG"
     gene            complement(4793..5725)
                     /locus_tag="PLV49_02990"
     CDS             complement(4793..5725)
                     /locus_tag="PLV49_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG97840.1"
                     /translation="MSLLDVNDLNVGFQTRLGTVRAVNNVSFEIDKGEVFGLIGESGS
                     GKSVIGMSLLHLLPRNARVRGRAIFEGSDLLALDEEKMQKFRGEKIAFIPQNPTASLN
                     PLMKNGLQASEIFEQKGYSRQEAWDRALGVMQSLLIREPEVSAKRYPHQLSGGMKQRL
                     VAGISLSLEPELVIADEPTKGLDSISRKRAIEFFQQLEGKERRSMLLITHDLDLAYEI
                     CDRLAVLYAGEIVEMGPARKLMDSPEHPYTRGLIKSLPKNGLEPLEGSSPSLLGLPDG
                     CFFRERCNCQTIDCRKWHPQLSLNNGWMKRCVLP"
BASE COUNT         1346 a         1567 c         1304 g         1422 t
ORIGIN      
        1 ccttatcatc aatcgcctcc tgcagttggt gcaagcttat cggcttagag ataaagccat
       61 ctcctccctc gctgatgcat tcatctctgc ggctggtgca gccagtgaca aagatgatcc
      121 tggggccctc tggccagcgt ttccggatga ttctagccgc ctgcaatcca tccatctctg
      181 gcatctgaat gtccatgagc accagattgt aagagcggct ttccatggca atcaaagctt
      241 cgaggccatt gtctactgta tcggcattat accccagcgt ctttagcatc cgcaaggcga
      301 ccagctgatt tgccgggtca tcctcggcaa tgagaatgct taaagctcta ttggggtgct
      361 tttcaaagac caaatcagat cctttttcaa atgtcatccc actcaccatt ttcccaagaa
      421 agcaattctc tctatggttg gagaattaat agtaatttgt attattaagt aataagcatt
      481 tcgattgtag taatatcaaa gcttttgtcg tcttgtattc caataaaatc cagaaaaaag
      541 aaaaatgagc ctgccggcct agagcacgat ctgcttattg tggtttagaa cctctgccat
      601 agtgtctgca ccctcaaccg atccagggaa gttggtgaag atcactcgct ctgcatcgat
      661 tcccttatcc ttcaaggact cttcaattcc cttggccagc tccccggact gcatattctc
      721 tatgatatat ttcacattct ggtatttctc aggattggca ttgatgtcgt ccaccactct
      781 ggccgcggtc ttggttccat tcagggcgaa ctcaggcgca aagaaattga ccacattcat
      841 gcccagccag ttttgcacag gatctttttg ccagagcatg actatgacct gcgtctggtt
      901 tagctgcttc ctctccgact ctgtaggctc gatggcgtca aagctttttg cataatcatt
      961 gtagcgctgc tcgtagtagg ttttgttggc cgggtcttta tttgcgagcc acccttctac
     1021 ctcggcagcc agcagcttgg agttggtcgg ggtgttccat ccgcgggaag ggttggatat
     1081 cgtcatccat tcagctgtcc catagttatt ggccgtcatg aaatcgttga tttccggcat
     1141 gttatatctt tggtcgttgc tgtcgttgta cgccataaac atgttagcat ctttgatgaa
     1201 atccatgttg agctgaatgc gatttggaat gatgtctgtt tgcaggtgag ggcacagggt
     1261 agggtcagcg atggaaatgg cctccaccct atctccgccg atataggttg ccggatccat
     1321 caatacactg gtggtgcaga ctatcttgag cgttgactgt gccgtttctg caccagcagg
     1381 aacgatcaaa aagatcgcca gcatcagtat ggctaagttg gatgattgaa ttctcataat
     1441 tgaaattagt aatactaaat atttaagttt tactactatt aacttaatta tctttgtaag
     1501 ttcgtattaa atataacgaa tcaagaaaca ttatctattt tagccaaaaa cgacctcact
     1561 atatcattgg ccatcttccg ggcgatctct gccgggattc ccatggccag gcagttttcg
     1621 ggcagccaac ccctggcgga aggatcggtt ggaccgatgg atatctgaga cagaaaagca
     1681 tttcccgcca tgcccgcagg ataggtaacc agggtagcgc aagccgggcc ccagggaacc
     1741 gatattaaat taaagacgtt atggctgccg aaatgagcca gtgcacataa atccctgatc
     1801 tgctcgccgc tggcaaaaca tgctatgcat tttacgccag ggtcctcctg gaaatcatcg
     1861 cagcgcctca tcaccacata tctgcccagg gttttgatct cacctactgc cttgtaggtc
     1921 tcgcatgcga tattttcgtt ctctttcaga tgctttccat cctgttttgc agnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttgcaggc aaggcaatcg ctgaacgaag
     2101 aggaccggac agcattttcc ggtacctgat ccgagccgta cacgcatacc ggactggtct
     2161 tcatattcac ggcatttgcc aggagcgatc ctatttgcct gattgacaat ctttccttga
     2221 ttattaccac caccatcaat gtatcttggt atttatcctt ctcttaaaag acagcattac
     2281 ggatattgca aatacacctg tgagcagcag ggccaccaga ggagcgacgg gcaacgtaaa
     2341 ctcaaagccc gcccagacgc caaaggcgct caccaagaga gctaccagcg gcgcaactat
     2401 gaacatgctc tttacattaa agcagaactg gccggctatg gatgcaggcg tcaccagcaa
     2461 tacgaatatc aagaggccgc ccacaatgtt gaggctgagc gccacggata gggcaataat
     2521 gaataatagt acgtaatata cgagcttaac ccggaccccg gctgactcgg cgatcttcat
     2581 gttgaacatg atggccgaga tctctttgta gaacgctaaa acgaagagga tggaaaggca
     2641 gcagatggca gccagggcat aaatctcctc ccggtataaa gatatgacat ccccaaatag
     2701 aaggctgctt gcggagagcc ctttgcccat atactccata taagagatca agaagatggc
     2761 cactgccatg gacagggaga ataaaacgct taaggtggca tcggccgcca tcttggcctt
     2821 ctcgctaacc ggaccgatga gagctgccac tcccatgctg aaggcgatgg cagccatctg
     2881 agcattcgct cccaggaaca tgcccagagc tgctccggca aaggcagcat gagacatggt
     2941 aaagccaatt gaggagaggc tcatcctgga gatgatcacc cccaggatgc tgcaggcgac
     3001 ggaggcaaag atcatcgcct ccagggcatg gcagaccaca ttattctcta tcagccactc
     3061 gagcatcttt ccttgacctc gcactccttt ctgattatct cctccacctc ctccgcctgg
     3121 cagctcaggt cgccggtgac cctgccccgg ttcatgacca ctagatgaat gggaatgtcn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccaccctcct tctcacctca
     3301 aagccacgtc ggcgaacatc caggccgcag acacaggccc taccatgggt gatcttgacc
     3361 aggccgttga tcgactccag cagggtggtc tttcccgcgc tgttcgggcc gccgatgacc
     3421 acatactctc cccgatcaac ctccagggaa agatccttga taaccggctc tgagccgccc
     3481 tcatatgcag tgtagatgct ctcaagaacg ataacactca aagacgctca cccatggtcc
     3541 caatcccgac gcaggtccgc agtaaagaga tctgcatcga ccatgcggta catcttctca
     3601 gccagctgct cgatgagcag gtcatggtct tttccgctct ctgagggaaa ggcaatcggc
     3661 ctgggaatga tgaccagctc cctctccccg gcggcgaaat ggcagcggat ctcgttgctg
     3721 gggacgatcc ggatgtttcg cttgcccagt gtgcagccac tgccgatctg cactccatcg
     3781 gcgaggcagg attgaggagg cttatcctgg cagaagacct ctgccttcat ctggaaggga
     3841 tcggagcaga ggttcttgcg ggcatatctg ccgatcatat agcccaggac gatgtaaggc
     3901 ccgagatggc catgaaatgc cgccaggtcc tcgatggtat aatctttatc tatgccagtg
     3961 tatatgcatt tgccctcttg gtggtccata atcagactgc ctccacatga tatgccttta
     4021 ttctgagttt aagtgctaca tattaaattg ttgtgcataa cagttaatac tacaatcctc
     4081 tgtcatcctc ttttggatct gctccatctc ttcttgcatt cgttcctctc gatgaggcta
     4141 atagatccct cctcgataaa ggcaatcctg tccgagaccg cccgcaagac atccagatca
     4201 tgagagatga atagatatgc cagaccagta tcctcttgca gccccatcat cagcctgagg
     4261 atctgagcct gaacagatgc atccagcata gatgtcggtt catccgccac gacgaattta
     4321 ggctcaagag acatgatcct ggccagcaca gccctctgaa tctctccccc gctgatctgt
     4381 tggggatatc tctccagatg ctccggccgc agacccacct gacggataag ttgctctagc
     4441 tcttcatcga tctcggatcg gcccaatctc ctgtgtatca gcagaggctc agctatgctc
     4501 tctttcaccc tcatcttggg attcaatgag gtttcaggat gctgaaagat catctgaagt
     4561 cgaggtctga ccccaaacac ctcccttcta ttcatcttga gcagatctct tcccttgaag
     4621 aggatctttc cgcagtccgg cttctccagg agcataatac atctgccgat agtggtcttt
     4681 cccgatccgc ttatcccaaa cagccccagg gtctctcctt cttctacaga gaagctaacc
     4741 tcatcgatca ccttctgcct cccgtattgc ttggagaggc tctgcacatc aatcagggta
     4801 gaacgcatct tttcatccaa ccattattca ggctcagttg ggggtgccat ttcctgcagt
     4861 ctattgtctg gcaattgcat cgctcccgga aaaagcaccc atcaggcagt cccagcaggc
     4921 tgggactgct tccctctaac ggttcaagac cgttcttcgg aagcgatttg ataaggcccc
     4981 tggtgtaggg atgctccggg gaatccatca gctttcttgc tggtcccatc tccacaatct
     5041 ctcccgcata cagaactgcc aatcgatcgc agatctcata agccagatca agatcatgag
     5101 ttatgagcag catggagcgg cgctctttac cctcaagctg ctgaaagaac tcgatggccc
     5161 tcttcctgga gatggagtcc agtcccttgg tgggctcgtc tgctatcacc agctccggct
     5221 ccagcgagag ggaaatccca gctacaagcc tctgcttcat ccctccagag agctgatgag
     5281 gatatctttt ggccgagact tccggctctc tgatcagcaa gctctgcata actcccagag
     5341 ccctatccca agcctcttgc cgggagtagc ccttttgctc aaagatctct gatgcctgca
     5401 gcccattctt catcagtgga ttgagggatg cagtggggtt ctgggggatg aaggcgatct
     5461 tttccccccg gaacttttgc attttctcct catctagagc caggagatcc gatccctcga
     5521 atatggctct ccctcgcaca cgggcattcc tgggcagaag gtggagcaga gacatgccta
     5581 tcaccgactt tcccgatccg ctctccccaa tgagcccaaa gacctcaccc ttatcgatct
     5641 caaacgatac gttgttcacc gctcgtacgg tgcccaatct tgtctgaaaa cccacattaa
     5701 gatcattgac atcaagcagc gacaaggccg ctcacctcgc tctcaggatc aagaatctcc
     5761 tggaatccgt ctcccagaag gctgaataga atcacagtca gagttatggc cattcccgga
     5821 aacagggtca gataaggag
//
LOCUS       DAOSGP010000063         5772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_12274_length_5772_cov_19.924086, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000063 DAOSGP010000000
VERSION     DAOSGP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12274_length_5772_cov_19.924086"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..633
                     /gene="psmB"
                     /locus_tag="PLV49_02995"
     CDS             13..633
                     /gene="psmB"
                     /locus_tag="PLV49_02995"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HOG97841.1"
                     /translation="MVEVFKGTTTVGVLCDGGVVLASESRATMGSFIASSQAKKIYQI
                     DDLVGMTTAGGVGDAQSLVRMVQVEARLYKMQRGEGLTVKAVTTMLANILSSRRYYPF
                     MVQLIMGGVDRYGPRIYSLDALGGQIEERRIVATGSGSPVAYGVLEAMYKPGISVEDG
                     TVLAARAIHNALKRDSASGDKIELVRITDKYEEVGEPEYSEIMNLI"
     gene            654..>1453
                     /locus_tag="PLV49_03000"
     CDS             654..>1453
                     /locus_tag="PLV49_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG97842.1"
                     /translation="MSVEEVLFELKRKVQSKLPPDINVTGLEFEGPELVIYTDDPKKV
                     ADDGEIIRNLAKELRKRVVVRPDLKVLADPESAIIKIQEVVPKEAVLTNYHFDGETGE
                     VQIEAEKPGLVIGRHGATLREITKLIGWTPKVVRTPPVRSSTIANIKDYLRSVQSERK
                     NILRTVGRKIHRDIASKDQWVRITTLGGCREVGRSCMLLSTPESRIIIDCGINVGSDD
                     SATPYLYVPEVYPLNQIDAVVLTHAHLDHAGLVPMLYKYGYEGPIYCTP"
     assembly_gap    1454..1553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1554..2039
                     /locus_tag="PLV49_03005"
     CDS             <1554..2039
                     /locus_tag="PLV49_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HOG97843.1"
                     /translation="INPFLAECFVQVESPKQRTEIVEGPPCVILATSGMLNGGPVIEY
                     LKRLGPDEKNTLVIVGYQAEGTLGRRIQKGWKEVPLSVDGKTQTVKINLEVTTVDGFS
                     GHSDRQQLMEYLRRIYPKPGRVLTNHGEEGNCLDLASSIYKRYRIPTMAPMNLETIRL
                     I"
     gene            2103..2621
                     /locus_tag="PLV49_03010"
     CDS             2103..2621
                     /locus_tag="PLV49_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOG97844.1"
                     /translation="MTGKRTRIINDPSDLVPLLQAFGSAVHKKIFDELSREWRTEREL
                     AGLFGDDAGVHRSVALLRKSGLIETKWRVPEPGESPEKEYHSTYSRIQANFQATMEDL
                     SELISLTFMSDEELREVTEKLQKMVNGGNNSLSNLSRELGLSQVFIRGVAKRAEGLAV
                     RGQRIEPYEEDF"
     gene            2621..3409
                     /locus_tag="PLV49_03015"
     CDS             2621..3409
                     /locus_tag="PLV49_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG97845.1"
                     /translation="MANVLQSKRESSRFQILVEIAAHQPNLRQKEVANKLGVTPQAIS
                     EYIKELVADGLVTTDGRMRYSITKEGVEWLLESAAELKRYARVVMEEIISHVSVWTAI
                     AESDLVEGERASLEMRGGLLYANKKEKIEASGIVIASASEGEDVGISDLRGLISLEEG
                     RITLCKVPRVQAGGSRGVDLEVLKGLLSEERMVGCLGIEALVALRKAGRKPDVLFGAK
                     EFAVEAAYHGVSSLVVSVDEQIPGLLTRLESEGLKYELIDLTLG"
     gene            3431..>5772
                     /locus_tag="PLV49_03020"
     CDS             3431..>5772
                     /locus_tag="PLV49_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5814 domain-containing protein"
                     /protein_id="HOG97846.1"
                     /translation="MTFQVLAQADRSRIQLLPQSGDKVLFEGHLRLKDMPQGPRVFKF
                     LVKKENEAERYLPPEDAMRLLRKAGAIYLVRGDSALEKRFLELLESYQLQYRFVQVCG
                     HCLGQKRVTYVGRDAITYKGARICENCAAAELLREADFHHLSRPAKSHLARILKKRRS
                     LDDVIGLLSLQRLEPELTRFDFIPASHEETGLPLDSIDLPGPLKELLRRRLTRLLPVQ
                     SRAISAGLLEGKSQLVVSATATGKSLIGEMAGIKNLLQGNGKLLFLVPLVALANQKYD
                     QLSAYNELGFQTSIRVGTSRIRLGKKRKIVQSLNADIISGTYEGIDQVIRTKKSLGRV
                     GTVVIDEVHMLEDAERGHRLAGMIARLRNAAPEAQFIFLSATVGNPGALAKRLNAALV
                     EYEVRPVPIERHLIFSSGKEKRMLLRQLVAQAEKLTSSTGYRGQTIIFTNSRKNCYSL
                     AQAIPGAAAYHAGLQYPERKRIEELFGQGKISTVVTTAALAAGVDFPASQVVFESLAM
                     GINWLNVHEFNQMLGRAGRPGYHDLGLVYILAEPGRRFSSGRGESEDEMALALLHGAM
                     EDVAPEFSDEQQQEEVLANAVAARSRQELEKLHDLTLGLDDLDSALNSLERAGLVKGF
                     EPTRLGAAAAAHFLTPDQTNLIAKRIGKGRSALEVAVELEGFEAIYLKFAEHISTKLH
                     MQVSQRALHGSFLDLLSSSDLNQLERKIQRYCLDFVRDFLRCTCKESPYCGCVQKSIS
                     LSILDLRAEGKSPEEIIDYFSDRYGMYAYQGDLINYLDQMV"
BASE COUNT         1334 a         1407 c         1722 g         1209 t
ORIGIN      
        1 tgaggtaatt aaatggtcga ggtgttcaaa ggtacaacta cggtaggtgt actctgtgat
       61 ggaggtgtag tccttgcatc ggagagccga gctaccatgg gcagcttcat agccagcagc
      121 caggccaaga agatctatca gatcgacgac ctggtgggta tgaccactgc cggaggtgta
      181 ggtgatgccc agtctcttgt ccggatggtt caggtggagg ccaggctcta taagatgcaa
      241 aggggcgaag gattgacggt caaggccgtc acaaccatgc tcgccaatat tctctcatcc
      301 aggcgctact atccttttat ggttcagctc atcatgggcg gtgtggacag atacggccct
      361 aggatttact ctttggatgc cctgggaggt cagatcgagg agcgaaggat tgtcgctact
      421 ggctccggtt ctccggtcgc ttatggcgta ttggaggcca tgtacaagcc gggtataagc
      481 gtcgaagatg gaactgttct ggcggctagg gcaattcata acgctttgaa gcgagattcc
      541 gcttctggtg ataagatcga gctcgttagg attacagata aatatgagga ggtaggcgag
      601 ccggagtatt cggagattat gaatctcatt taatcttttt tggatgtgta atattgtcgg
      661 tagaagaagt actttttgag ctcaaacgca aggttcagag caaactgccc ccggacatca
      721 acgtcacggg gctggagttc gagggtccgg agctggtcat ctacaccgat gaccccaaga
      781 aggtggctga tgacggtgag atcataagga acctcgccaa agagcttcgc aagagagtgg
      841 tggtccggcc ggacctgaag gtattggcag atcctgaatc tgctataatc aagatccagg
      901 aggtcgtgcc caaagaggca gtcctcacca actaccactt cgacggcgag accggtgagg
      961 tgcagatcga ggcggagaag cccgggctgg tgatcggccg ccatggtgcc accctgcggg
     1021 agatcacaaa gctgatcggc tggacgccga aggtggtcag aaccccgccg gttagatctt
     1081 cgaccatcgc taatatcaag gattacctgc gcagcgtgca gagcgagaga aaaaacatcc
     1141 tgcgcacagt gggcaggaag atccaccggg atattgcttc caaggaccag tgggtcagaa
     1201 taaccacctt aggcggctgt cgggaggtgg gcagaagctg tatgctcctc tccactccag
     1261 agtcccgtat catcatcgac tgcggaataa atgtgggatc ggatgactca gccaccccat
     1321 acctctatgt gcctgaggtc tatcccttga accagatcga tgctgtggtc ctgacccatg
     1381 ctcacctgga ccatgcaggc cttgttccca tgctctataa gtacggctat gagggcccca
     1441 tctactgcac tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcaatc
     1561 cattcctggc ggagtgcttt gtccaggtgg agtctcccaa gcagaggacg gagatagtcg
     1621 aagggccgcc ttgcgttata ctggcgacga gcggcatgct caatggcgga ccggtgatcg
     1681 agtatctcaa gcgcctggga ccggatgaga agaataccct ggtcatcgtc ggctatcagg
     1741 cagagggaac ccttggcaga cggattcaga agggctggaa ggaggtcccg ctatctgtgg
     1801 atggaaagac ccagacggtg aagatcaacc tggaggttac aacagtcgat ggcttctccg
     1861 gtcactccga tcgccagcag ctcatggagt acctgaggcg catatatccc aagccaggcc
     1921 gggttctaac caaccatggt gaggagggca actgtctgga tctggccagc tccatctaca
     1981 aaagataccg gattcccacc atggctccca tgaacctgga aaccatcagg ctgatctgat
     2041 cttcaaaagc tttatcaaag aaaaggcata atgtcaatcc tagttattac cgtgaggttc
     2101 ttgtgactgg aaaaagaacg cggatcatca atgatccatc agatctggtt ccattattgc
     2161 aggcctttgg ctcagccgtt cataagaaga tattcgatga gctaagccgg gaatggcgaa
     2221 ctgagcggga gctagctggc cttttcggcg atgacgctgg cgtccacaga agcgtagccc
     2281 ttctgcgcaa gagtggcctg atagagacga agtggagggt gcctgagccc ggggagagcc
     2341 cggagaaaga gtatcattcc acctactctc gcatccaggc caacttccaa gcgaccatgg
     2401 aggatctgag cgagctgatc agccttactt ttatgtccga tgaggagctg agggaggtaa
     2461 ctgagaagct gcagaagatg gttaatggag gcaacaactc gctttctaat ctttcccggg
     2521 agctgggact cagtcaggta ttcattcgtg gcgtggccaa gagggcagaa ggactggcgg
     2581 tgcgaggaca gcggatagag ccttatgaag aggatttctg atggccaatg tattgcagag
     2641 caagagggaa agctctcgtt ttcagattct ggttgagatt gctgcccatc agcccaacct
     2701 ccgccaaaag gaggtggcaa acaagcttgg tgtcacccct caggcgatct ccgagtacat
     2761 caaggagctg gtggcagacg gcctggtaac tacagacggc cgcatgcgct acagcatcac
     2821 caaagagggc gtggagtggc tgctggagag cgctgctgag ctgaagcgat atgctcgggt
     2881 ggtgatggag gagatcatca gccatgtgtc cgtctggacg gcgatagctg aatccgattt
     2941 ggtcgaggga gagagggcct ccttggagat gagaggcggc ctattgtatg ccaacaaaaa
     3001 agaaaagatc gaggccagcg ggattgtcat tgccagcgcc agcgaagggg aggatgtggg
     3061 catctcagac ttgcggggat tgataagcct ggaggagggg aggataaccc tatgcaaggt
     3121 tccgcgagtg caagcaggcg gatcgagagg ggttgacctg gaggttttga aaggtctgct
     3181 ctcagaggag aggatggtgg ggtgcctggg aattgaggcc ctggtggctc tgaggaaggc
     3241 cggcaggaag ccggatgtgc tcttcggtgc caaggagttc gcggtggagg cggcatacca
     3301 tggagtcagc tctctggtgg tgagcgtgga cgagcagatc cccggccttt taacccgcct
     3361 ggagtccgag ggcctgaagt acgagctcat agacctcact cttggctgaa tcgaactcgc
     3421 agattcaaag atgacttttc aggttcttgc tcaggcggac aggtctcgca tacagttatt
     3481 gccccagagc ggggacaagg ttctcttcga gggccatttg cgcttgaagg acatgcccca
     3541 ggggccaagg gtcttcaagt tcctggtcaa gaaggagaat gaggccgaga ggtaccttcc
     3601 tccagaggat gctatgaggc tgcttcgcaa agctggggcc atctatcttg tcagggggga
     3661 ctcggctctg gaaaaaaggt tcctggagct attggagtcc taccagctac agtaccgctt
     3721 cgtccaggtc tgcggccact gtctggggca gaagagggtg acctatgtgg ggcgagatgc
     3781 cataacctac aagggcgccc gcatctgcga gaactgtgca gcagcagagc ttctccgaga
     3841 ggccgatttt catcatctga gccgtcctgc caagtcccat ctggccagga tcctgaaaaa
     3901 gaggcgatct ttggatgatg tcatcggcct gctatctttg cagaggctcg agcctgagct
     3961 aacccggttt gatttcattc ccgccagcca tgaggaaact ggtctgcccc tggattcaat
     4021 cgacctgcct ggacccctta aggaactgtt gcgtcgcagg ctgacaagac tcctgcccgt
     4081 ccagtccagg gcaatctcag ccggcctcct ggaaggaaaa agtcagctgg ttgtatcagc
     4141 tacagctact ggtaagagcc tgatcggaga gatggcgggg ataaagaacc ttttgcaggg
     4201 aaatggcaag ctactgtttc tggtgcctct ggtggccctg gccaaccaga agtacgatca
     4261 gctctccgcc tataatgaac tgggctttca gacctccatt cgggtgggaa caagtcgcat
     4321 ccgtctggga aagaagagga agatcgtcca gagccttaat gctgacataa tatccggcac
     4381 ctacgagggc atagatcagg tgatccgcac caaaaagagc ctggggcggg tgggaacggt
     4441 ggtgatcgat gaggttcata tgctggaaga tgctgaacgc ggccatcgtc tggctgggat
     4501 gatcgcccgc ctgagaaatg ctgccccaga ggcgcaattc atcttcctct ccgccacagt
     4561 aggaaatccg ggggccctgg ccaagcggct gaatgcagct ttagtggagt acgaggtgcg
     4621 gccggtgccc attgagcgcc atctaatctt ctcctcggga aaagagaagc gaatgctctt
     4681 gcgccagttg gtcgcccagg cggagaagct gacatcgtct accggctacc gtggccaaac
     4741 cataatcttc accaactctc gcaaaaactg ctatagcctc gctcaggcga tccccggggc
     4801 ggcagcttat catgccggcc tgcagtatcc agaaaggaag agaatcgagg agctatttgg
     4861 ccagggaaag atcagcactg tggtgacgac cgcagcgttg gccgccgggg tagactttcc
     4921 cgcctcccag gtggtcttcg agtccctggc catgggcatc aactggctga atgtgcacga
     4981 gttcaatcag atgctgggaa gggcaggcag gccaggttat catgacctgg gattggtcta
     5041 catcttggcc gagccgggcc gacggttctc ctctggtcgg ggggagtcag aggatgagat
     5101 ggcacttgcc cttctgcacg gcgctatgga ggatgtggcc ccggagttct cggatgagca
     5161 gcagcaagag gaggtcttgg ccaatgctgt cgctgccaga tcccgtcagg agctggagaa
     5221 gctgcatgat ctaaccctgg gtctggatga tctggatagc gccctgaatt ctctcgagag
     5281 ggcaggtttg gtcaaaggct ttgagcccac ccgcctcgga gcggcagcag cagcccattt
     5341 tctcacccca gatcagacaa atctcatcgc taagaggatt ggcaaaggaa gatccgcttt
     5401 ggaggtggca gttgaactgg agggttttga ggctatttat ctgaagttcg ccgagcatat
     5461 atccaccaag ctgcatatgc aggtctccca gagggctcta catggctcat ttttggatct
     5521 attaagcagc tccgacctga accagctgga gaggaagatt cagagatact gcctggactt
     5581 cgtccgcgac tttctccgct gcacctgcaa ggagtctccc tactgcggct gcgtccaaaa
     5641 aagcatatca ttgtccattc tcgatctgag ggcggaaggc aagagcccgg aagagatcat
     5701 cgattacttc agcgatagat atggaatgta tgcctaccag ggcgatctga tcaactattt
     5761 agaccagatg gt
//
LOCUS       DAOSGP010000064         5730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_12389_length_5730_cov_17.025022, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000064 DAOSGP010000000
VERSION     DAOSGP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12389_length_5730_cov_17.025022"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLV49_03025"
     CDS             <1..613
                     /locus_tag="PLV49_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97847.1"
                     /translation="MSDRARELEAAAASIDAASLDTARKGIVTGCQELIYWLELLSRR
                     LEKVPPEKEHKFARAFSLIMLGHLPTRPETCPFCVQYGQSRSCRGCGYAATHGRCDSD
                     QSSFSLFIEAFSELGRVIYQDTGGLNCHPDDARLRLEHCIRSSRLLAADMMEDIDSSS
                     AERLMERKARYLGQMIDLLPKELFGPEIMESWRRVHEMLRNYW"
     gene            complement(648..1742)
                     /locus_tag="PLV49_03030"
     CDS             complement(648..1742)
                     /locus_tag="PLV49_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG97848.1"
                     /translation="MGKVFVSPLNWGLGHSTRDIPIIEELIRRGHEVTIGTSGNALTL
                     LKREFPECDFITFKDYPAPYSSTRFFLPKFAASIPILLRALARERKKLDEILTKNKFD
                     LIISDNRMGVYSHDIPSYFISHQLRFSLPQYLYPFEMLTIPVNSFVHVKFDGVIVPDI
                     YPWAGQPNLSGKLSRSRLDATNKRAYFAGIFTSTRKMNLDEDLDYLIIVSGPEPQRTK
                     LEEIILKQVQKLPGEKVVLLGSPQKERHEKLDEHTTVHSYLSTEDKVELMNRARFLIT
                     RSGYTTMMEMVELDKKHGLFIPTPGQTEQEYLSRYYARQGWFFSRSQYKLRLPQDVQE
                     AMNYQGFPEMTKTAENVKRLYEELFVQHLS"
     gene            1820..2074
                     /locus_tag="PLV49_03035"
     CDS             1820..2074
                     /locus_tag="PLV49_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97849.1"
                     /translation="MYEQIVQAVDKMKEGSPGYEGISAILNRYARGEIDLDEAYYDLL
                     EAELIAMPKRCGMSAKRPVTAEDELRLKEKILEKIKEDLH"
     gene            2128..>2340
                     /locus_tag="PLV49_03040"
     CDS             2128..>2340
                     /locus_tag="PLV49_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97850.1"
                     /translation="MLGISVTELRDLVVSLIALVIAFSVLFGGAVLDMNMIIVSTLGV
                     ASGFLLHELAHKFTAQHFGYLAEYRAN"
     assembly_gap    2341..2440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2514..2744
                     /locus_tag="PLV49_03045"
     CDS             2514..2744
                     /locus_tag="PLV49_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97851.1"
                     /translation="MTQIEVLQEISDTLISINDCLEGIGEELEEISMASKMMIFFKLI
                     ELKPEMKDKLGPLINELAESMDFTMDEAAGEE"
     gene            2961..>3637
                     /locus_tag="PLV49_03050"
     CDS             2961..>3637
                     /locus_tag="PLV49_03050"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOG97852.1"
                     /translation="MDEHVLTRLRQELIEGIDEKTRDGASRFFKEEVRFYGVKSSLVR
                     KIATKYFREIKGRDKREIFTLCEELLRSDYGEEAFIAYEWAYSLRSQYEPSDFTIFEG
                     WLERYVNNWAKCDTLCNHTLGSFIELYPQYMEKLFGWARSDNRWVRRASAVTLILPAR
                     RGDFLPEVFMIADILLLDKDDMVQKGYGWLLKEASRAHQAEVFDYVMKNKAVMPRTSL
                     RYAIEKMP"
     assembly_gap    3638..3737
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3738..5725)
                     /locus_tag="PLV49_03055"
     CDS             complement(<3738..5725)
                     /locus_tag="PLV49_03055"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOG97853.1"
                     /translation="MIERIRKRDGRVVEFQTEKIALAISKAFRAVGREEEDAPFRLAE
                     EVAKEAEERFSRTIPGVEEIQDLVERALISAGYADVAKAYILYRQKRTEIREAKRYLG
                     VADDLKLSVNAAEVLKKRYLLRDERGDVVETPGEMFGRVADHISKAESKYGGDVESYR
                     SLFLQMMRNLEFLPNSPTLMNAGLPLGQLSACFVLPVEDSLPSIFDALKSMALIHQSG
                     GGTGFSFSRLRPKDDVVRATGGVASGPVSFMRIFDTATDVIKQGGRRRGANMGILRVD
                     HPDIMEFIEAKERPGFLENFNLSVAATDEFMEKSRGEGEIDLINPRTGLAAGRLKAAE
                     LLGLIATSAWRGGDPGIIFIDRINAAHPLPGEIEATNPCGEQPLLPYESCNLGSINLS
                     RLVRKGELDWDRLAELVSLAVRFLDDVIDMNRFPLPQIEEATLSRRKIGLGVMGFADM
                     LIMMNISYASPQAVALAEKLMKFITEHARAESARLGEVRGSFPLFEKSRLRGYSSMRN
                     ATVTTIAPTGTISIIAGTSSGIEPLFALAFVRRVLEGARLLEVSPLFEKMAMERGIYT
                     PDLKAELARRGSVQDIAGLPQDLKELFLTALDTSPQQHVRIQAAFQKYTDNAVSKTVN
                     LPAEATVSDVLQVYRLAYDLGCKGVTVFRYGSRSQVLYL"
BASE COUNT         1210 a         1488 c         1567 g         1265 t
ORIGIN      
        1 tatgagcgat agagcaagag aactggaggc ggcggcggca tcgatcgatg cagcctccct
       61 tgatacggcc aggaagggga tcgtcaccgg ctgccaggag ctgatctact ggctggaact
      121 cttatcccgc cgccttgaaa aggtcccgcc cgaaaaggag cataagttcg cccgcgcatt
      181 ctctcttata atgctcggcc acttgcccac ccggcccgag acctgccctt tttgcgtcca
      241 gtacgggcag agccgaagct gccggggttg cggctatgct gccactcacg ggcgctgcga
      301 ttcggatcag tcgtctttca gcctgttcat cgaggccttc agtgagttgg gaagggtgat
      361 ttatcaggat acaggaggcc tgaactgcca tccggatgat gcccggctga ggcttgagca
      421 ttgcattcgc tcctctcgcc tactggcagc ggatatgatg gaggatattg attcctcctc
      481 cgccgagcgg ctgatggaga ggaaggccag gtaccttggg cagatgatcg atcttttgcc
      541 gaaagagctc ttcggcccgg agataatgga gagttggcgt cgcgtgcatg agatgcttcg
      601 caattactgg tagtactcag tctgctgtaa aatagtcaaa tcacatttca gctcagatgc
      661 tgcacgaaga gctcctcata gagcctcttg acgttctccg ccgtcttagt catctccggg
      721 aagccctggt agttcatcgc ctcctgcacg tcctggggaa ggcggagctt gtactggctg
      781 cgggagaaaa accagccttg ccgggcatag tagcgggaga gatactcctg ctccgtctgg
      841 ccgggggtgg ggatgaacag cccatgcttt ttgtccagct ccaccatctc catcatggtg
      901 gtatagccgg acctggtgat cagaaagcgg gcccggttca tcagctccac tttatcctcc
      961 gttgataggt aagagtgaac tgtggtgtgc tcatccagct tctcgtgcct ctccttctga
     1021 gggcttccca ggagcactac cttctccccg gggagctttt gcacctgctt taagatgatc
     1081 tcctccagct tcgtcctctg gggctcgggt cctgagacaa tgatcagata atccaggtct
     1141 tcatccagat tcatcttcct ggtgctggtg aagattccgg cgaaataggc tcttttgttg
     1201 gtggcatcca ggcgtgatcg gctgagcttg ccgctcaggt tcggctggcc cgcccaggga
     1261 taaatatccg gcacaatgac tccgtcgaac ttgacatgaa cgaagctgtt cacgggaatg
     1321 gtgagcatct caaaggggta gaggtactgg ggcaggctga agcggagctg atgggagatg
     1381 aagtagctgg gaatgtcgtg gctgtagacc cccatgcggt tgtcgcttat tatcagatca
     1441 aacttattct tagtgaggat ctcgtccagc ttctttctct ccctggccag agcgcggagg
     1501 aggatgggaa tgctggcagc gaacttgggc aggaagaacc gggtggagct gtagggggca
     1561 ggatagtcct tgaatgttat gaaatcgcat tccgggaatt ccctcttcag aagggtcagg
     1621 gcattgccgc tggtgccaat tgtgacctcg tggccccttc gtattagctc ctcaatgatg
     1681 ggaatgtccc tggtggagtg gcccaaaccc cagttcagag ggctcacgaa tacttttccc
     1741 atgaggcggg agatcaagct ctaggtataa atcgttttct tgataagaga taaatatcaa
     1801 cctgttccac caatagcccg tgtacgagca gatagttcaa gccgtcgaca agatgaagga
     1861 gggaagccct ggctatgaag ggatcagcgc catcctgaat agatatgcca ggggcgagat
     1921 cgatctggat gaggcctact acgacctctt ggaggcggag ctgattgcta tgcccaagag
     1981 gtgcggcatg tccgcaaagc gcccggtgac tgcagaggac gaactgcgcc tgaaagagaa
     2041 gatcctggag aagatcaaag aagatctcca ttagacggcc aacagagatg caaagcaaaa
     2101 gcggcaaggg taacaggtca agagcatatg ttgggaataa gcgttactga attgagggac
     2161 ctggtcgtat cattgatcgc cctggtaata gccttctcgg tgctttttgg aggggcggtg
     2221 cttgatatga atatgatcat cgtctccact ctgggtgttg ccagcggatt tctcctccat
     2281 gaactggctc ataagttcac tgcccagcat tttggatatc tggccgaata cagggccaat
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tctgcaattg tgctgctatc
     2461 gagggtcagt tatttcaata ctccatgcag gacgggaagt aggtgttgaa tgaatgactc
     2521 aaattgaggt tctgcaggag atctccgata ccctcatttc aatcaatgat tgcctggaag
     2581 ggattggtga agagcttgag gaaatcagca tggccagcaa gatgatgata ttcttcaagc
     2641 tgatcgaatt gaagccggag atgaaggaca agctcgggcc tttgatcaat gaactggcag
     2701 aatctatgga tttcacgatg gatgaggcag caggagagga atagcgaaaa agccctcact
     2761 gaaagggccg cctgagaaat tcagggagga ggtgggcccc gaatctctcg cgcatctgat
     2821 gatttgattt acttaacaaa aacatcatag atgtttattt tatttagccc tttccatttg
     2881 agaatgctcg ccctcccatt tttcttgagc cctcctttca gcgaagatct tttgatctaa
     2941 gcgattctgc agttgaggac atggatgagc atgtcctgac caggttacgc caagagctga
     3001 ttgagggaat cgatgagaag accagagacg gagcatcgcg ttttttcaaa gaggaggtca
     3061 ggttttacgg tgtcaaatcc agcctggtca ggaagatcgc gaccaaatac ttccgggaga
     3121 taaaagggag ggacaaaagg gagatcttca ccctgtgcga agagctcctc cgctcagact
     3181 atggggagga ggcgttcatc gcatatgaat gggcctattc cctgcgcagc caatatgagc
     3241 cctccgactt cacaatcttc gagggctggc ttgagcggta tgtgaacaac tgggcgaagt
     3301 gcgataccct atgcaaccat accctgggaa gcttcattga attgtatcct caatacatgg
     3361 agaagctctt tggctgggcc cggtcagaca accgatgggt gcggcgggcc tcggctgtga
     3421 ccctcatcct ccccgctcgc aggggcgact ttctgccgga ggtcttcatg atcgctgaca
     3481 tcctccttct ggataaggac gacatggtgc agaagggata tggctggctt ttaaaagagg
     3541 cgagccgggc tcatcaggct gaggtcttcg actatgtgat gaaaaacaag gccgtcatgc
     3601 ccagaacgtc tctgcgctat gccatagaga agatgccnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnaga tagagcacct ggcttcggct tccatatctg aataccgtta
     3781 ctcccttaca ccctaagtcg taagccagtc tgtaaacctg gagcacatcg cttaccgttg
     3841 cctctgcggg caggtttacc gtcttggaga cggcattgtc cgtatatttt tggaaggcgg
     3901 cctgaatgcg cacatgctgc tggggggagg tgtcaagggc ggtgaggaaa agctccttca
     3961 aatcctgggg caggccagcg atatcctgta cgctgcccct ccgggccagc tccgctttga
     4021 gatctggggt ataaatcccc ctttccatcg ccatcttctc aaagagcggt gagacctcca
     4081 gcagccgcgc cccctccagc acccggcgca cgaatgccaa tgcaaagagc ggctcaatgc
     4141 cgctgctcgt gccggcgatg atgctgatgg taccggtggg ggcaatggtg gtcacagtgg
     4201 cgtttctcat gctgctatag ccccttaggc gtgacttttc gaagagtgga aaggagcccc
     4261 ggacctcccc gaggcgggcc gactcggccc gagcatgctc ggtgatgaat ttcatcaact
     4321 tctccgccag ggccacagcc tggggcgagg cgtaggagat gttcatcatg atgagcatat
     4381 ccgcaaagcc catcacaccc aggccgatct tgcggcggct cagtgtggcc tcttctattt
     4441 gcggcagggg gaagcggttc atatcgatga catcatccag aaacctcacc gccaggctga
     4501 ccagctcagc caaacgatcc cagtccagct ctccttttct taccagccgg gagaggttga
     4561 tcgagcccag gttgcaggac tcatacggca gcaagggctg ctccccgcag gggtttgtgg
     4621 cttcgatctc cccagggagg gggtgggcgg cattgatgcg atcgatgaag atgatccccg
     4681 ggtcgccacc cctccaggcg gaggtggcga taaggcccaa aagctccgcc gccttcagcc
     4741 ggcccgccgc caggcccgtg cgcggattga tcaggtcgat ctcgccctca ccccgcgatt
     4801 tttccataaa ttcgtccgtc gccgccacag agaggttgaa gttctccagg aatcccggcc
     4861 gctccttggc ctcgatgaac tccatgatat cgggatggtc gaccctcaat attcccatgt
     4921 tcgctccccg gcggcgcccg ccttgcttga tcacatcggt cgccgtatcg aagatgcgca
     4981 tgaaagagac cggtccgctg gccactccgc ctgtagcccg gacgacatcg tccttgggcc
     5041 gcagtcgaga gaaggagaat cccgtgcctc cgccgctctg gtggatgagg gccatgctct
     5101 tcagagcatc aaagatactg ggcagcgaat cttctacagg aaggacaaag caggcggaca
     5161 gctggcctaa aggcaagccc gcattcatca gcgtggggga gttgggcagg aactccaggt
     5221 tcctcatcat ctgcaggaaa agagatcgat agctctcaac atcgccgcca tatttggact
     5281 cggccttgga gatatgatcg gccacccgtc cgaacatctc tcccggggtc tccaccacat
     5341 ctccccgctc gtcccttaag agatacctct tcttcaacac ctccgcagca ttgacggaga
     5401 gcttcaggtc atctgccact cctagatagc gtttggcttc tctgatctcc gttctttttt
     5461 ggcggtagag gatataggcc ttggccacat cggcgtagcc cgcggatatg agcgctcttt
     5521 ccaccaggtc ctggatctcc tccaccccgg gaatggtgcg ggagaatcgt tcctctgcct
     5581 ctttagccac ctcctctgcc agcctgaaag gagcatcttc ctcctctctg cccacggctc
     5641 gaaaggcctt ggatatggcc agggcaatct tctcggtctg gaactcgacc acccttccgt
     5701 cccgttttct gatgcgctcg atcatccctg
//
LOCUS       DAOSGP010000065         5685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_12542_length_5685_cov_20.850977, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000065 DAOSGP010000000
VERSION     DAOSGP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12542_length_5685_cov_20.850977"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..416
                     /locus_tag="PLV49_03060"
     CDS             3..416
                     /locus_tag="PLV49_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HOG97854.1"
                     /translation="MDKISIRAFVAATEDEGRVREALSIFVPLDHISSQTALGHFGNE
                     IKILEASLRRKEAQAFFQILHEQLPSEDSFRLHHEIPERLEGASHFHLRLDKQAAYKG
                     LFRLTESKDALDICALVKTYPSNRQQAIKILSELI"
     gene            413..1090
                     /locus_tag="PLV49_03065"
     CDS             413..1090
                     /locus_tag="PLV49_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HOG97855.1"
                     /translation="MSYFECLRAYPEGSSSASRMALKAKSLGYGGIIICNTAPGNLFR
                     PEAAERIRGIEVAIGAEVMAKDVRSLRSRITSLRPRYPLLLVRAATEELVRTASEDPN
                     VDVLIPSYSSRRPLGIAAARAAKLNQVAIGFDLGPFFRLSGRHRSRWLESLGRNLQLA
                     RKFDLRLLLTVGARSHLDLRPPRDIIALAEVMGFEHQEACRALSLAGDIMALNRRCWP
                     SPGVELL"
     gene            1129..1626
                     /locus_tag="PLV49_03070"
     CDS             1129..1626
                     /locus_tag="PLV49_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HOG97856.1"
                     /translation="MIFQLEAEGEIEGKDLIKEIIASQHSLFGDIGSAKNRLHLISFD
                     GRQGILRFHHKHMDQTRATLASIHSIQGTRAALQAKGVSGTIKSATEKYLPRLCKING
                     DCDRRRIELKEVSGCIIRTQGLEVDLCPDDSEWTRGSDTRYLGLTSFDLCGGHEDADG
                     TADGL"
     gene            1604..2371
                     /gene="psmA"
                     /locus_tag="PLV49_03075"
     CDS             1604..2371
                     /gene="psmA"
                     /locus_tag="PLV49_03075"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HOG97857.1"
                     /translation="MAPQMGYDRAITVFSPDGRLFQVEYAREAVRRGTTAVGIKVKDG
                     VAVLVDKRITSRLMEPKSIEKIFQIDSHIGAATSGLVADARVLIDRARVESQINRLVY
                     DEPIGVEALAKKICDFKQQYTQYGGVRPFGTALLIIGAEEDRARLFETDPSGALLEYK
                     ATGIGAGRSSVMEVFEEKYREDLTMDEAVLLGLEALHRAAEGKVEASTTEIGIIKLSD
                     KLFYKLTEKEVSDYVERMKAIVASNKVEPGKGEEGEA"
     gene            2374..3072
                     /locus_tag="PLV49_03080"
     CDS             2374..3072
                     /locus_tag="PLV49_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HOG97858.1"
                     /translation="MVRLDDAVPARLKTHGTTFEVLVDPDGALALKRGDSVNLEDILA
                     VEDVFENASRGDRSPEEDLQKAFGTTDPLSIAPIIIKKGEISLTADQRKRFLENKRRQ
                     VIEVIARNAINPQTKTPHPPGRIDQAMTEARVNIDPTKSTDELVKIVMKAIRPLIPIR
                     FEEVEVAVKVPPEYAPKAYGEISAFGRLTRESWQNNGAWIGVVQIPAGMQTEFYDLVN
                     RLTKGEAETKLLKH"
     gene            3091..3756
                     /gene="rrp4"
                     /locus_tag="PLV49_03085"
     CDS             3091..3756
                     /gene="rrp4"
                     /locus_tag="PLV49_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HOG97859.1"
                     /translation="MDRKVVVPGDLLSEDAKRSGEGTYIKDGNVYSLLYGLANYRDKI
                     NVIPLAGKYMPAPGDNVIGVVKDITFSNWIVDINSPYDGLLHISEFPKRIDSDEMSKY
                     LRIGSSIMTRVKDVDPTMKVELTLNDRKLGPIRTGQVIEISHTRVPRLIGKGGSMISM
                     LKKEVNCSIFVGQNGRIWINGCADDTDLALKTIALIEREAHTNGLTDRIVSFLKAEKE
                     ARS"
     gene            3762..4514
                     /gene="rrp41"
                     /locus_tag="PLV49_03090"
     CDS             3762..4514
                     /gene="rrp41"
                     /locus_tag="PLV49_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HOG97860.1"
                     /translation="MDENDISFFKDGLRLDGRRADELRPVKIEVGILARADGSCYIEM
                     GGNKVIAAVYGPREVHPRHLQEVTRAIVRYRYNMASFSVEERKRPGPDRRSYELSKVS
                     REALEPVILTSFFPRSVIDVFVEVLQADAGTRTAGINAAAVALADAGIPMKSMISSCA
                     AGKVGDTIVLDPMKEEDNFGQADLPIAMTPNGDITLMQMDGNLTPDEFGRAVMMAMKG
                     AKDIYELQRRALVAKYSQISADSAREEVHLCE"
     gene            4507..5283
                     /gene="rrp42"
                     /locus_tag="PLV49_03095"
     CDS             4507..5283
                     /gene="rrp42"
                     /locus_tag="PLV49_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HOG97861.1"
                     /translation="MSSVISEIKKDFIYNLLIKGERIDGRKFDQYREISIERDVIRKA
                     EGSALVKLGSSQVLVGVKMQPGEPFQDSPNRGVIITNAELVPLASPSFEPGPPNEVGI
                     ELARLVDRGVRESKAVDLEALCIEPGKKVWIVFIDVHILDDCGNILDAASLGAIAALL
                     CTKVPASRFGLGEDYILPIRDIPIATTAIEFSDVLMFDPGVEEEAIANTKLTVITTTN
                     GDICGMQKSGTGMLNPEQVYRIIDIACEKAKEIREKFLEA"
     gene            5299..5580
                     /locus_tag="PLV49_03100"
     CDS             5299..5580
                     /locus_tag="PLV49_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HOG97862.1"
                     /translation="MAKQTTKGKKSRSAARFGARYGRKSRKLVADLEEKSKASYDCPQ
                     CGRNKVKRTSTAIWSCRKCGYTFAGGSFLPETSLGRSVLRSLKKSLEAD"
     gene            5584..>5685
                     /locus_tag="PLV49_03105"
     CDS             5584..>5685
                     /locus_tag="PLV49_03105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001606.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HOG97863.1"
                     /translation="MAYKCTRCKKIVEIDYEYSGIRCPYCGHRILMKE"
BASE COUNT         1466 a         1418 c         1670 g         1131 t
ORIGIN      
        1 tgatcgataa aatctctatt cgggcctttg tggcagcgac ggaggacgag gggcgtgtgc
       61 gcgaagctct gagcatcttc gtccctctgg accatatatc ctcccagact gctttaggcc
      121 atttcggaaa tgagatcaaa atcctcgagg cttctctgag aagaaaggag gctcaggctt
      181 tctttcaaat tttgcatgag cagctgccct cagaggacag ctttcgcctt caccatgaga
      241 taccagagcg gctggaggga gcgagccact tccatcttcg cttggacaag caggccgcct
      301 acaaaggcct cttccgcctg acggaatcca aggacgctct ggacatctgc gctctggtca
      361 agacctatcc ctccaatcgc cagcaggcca tcaagatcct aagtgagctc atatgagcta
      421 ttttgagtgc ctgagagcct atcctgaagg atcgtcctcc gccagccgca tggcattgaa
      481 ggcaaaaagc ctgggctacg ggggaatcat aatatgcaac acagcccccg gcaatctctt
      541 ccggccagaa gcagcagaga ggataagagg gattgaggtt gccatcgggg cggaggtgat
      601 ggcaaaggat gtcagatccc ttcgcagcag gattacatct cttcggccga gataccctct
      661 cctcctggtt agggctgcaa cggaggagct ggtgcgcaca gccagcgagg accccaacgt
      721 cgacgtatta atcccctcat atagcagccg ccggccgctg ggcatagctg ccgcccgcgc
      781 cgccaagcta aaccaggtgg caataggatt tgacctcggt cctttctttc gtctatcggg
      841 tcggcatcgc tcccgctggc ttgagtcact gggacggaac ctgcagctag cacgcaagtt
      901 cgatctgcgc cttctgctca cagtcggggc caggtcccac ctggaccttc gtcctccccg
      961 tgacatcatc gccctggcgg aggtgatggg cttcgagcac caggaggcct gccgggccct
     1021 ttccctggcc ggtgatatca tggctctgaa tcgcagatgc tggccttctc cgggggtaga
     1081 gctactttga gaagcagact gcccgtcctc cgaatgagga ggagatatat gatattccag
     1141 ctggaggcag aaggagagat agagggcaag gatttgatca aagagatcat agccagccag
     1201 cactccctct tcggcgatat cggcagcgca aaaaacaggc tccatctgat ctccttcgat
     1261 ggccgccagg gcatactgcg ctttcatcat aagcatatgg accagaccag ggccacttta
     1321 gcctcaattc attccatcca gggaactaga gcggctctgc aggcaaaagg ggtctcgggg
     1381 acgattaaat cggccacaga aaagtattta ccacgtctat gcaaaataaa cggcgattgc
     1441 gacagaagaa gaatcgagct gaaagaagtt tccgggtgca ttattcgcac ccaaggccta
     1501 gaggtcgatc tctgtccaga tgacagcgaa tggacccgag gttcagatac cagatacctg
     1561 ggactgacct ctttcgattt atgtggagga cacgaggatg cagatggcac cgcagatggg
     1621 ttatgatagg gctattactg tattcagccc tgatggcaga ctatttcaag ttgaatatgc
     1681 ccgcgaggca gtccgtagag gaactacggc agtaggcatt aaggtaaagg atggtgtagc
     1741 tgtgctggtg gataaaagaa tcactagcag gctaatggaa cccaaatcga ttgagaagat
     1801 attccaaatt gacagccaca tcggcgcagc cacatccggc ctggtagccg atgccagggt
     1861 gctgattgac cgggccaggg ttgaaagcca gatcaaccgc ctggtttatg acgagccgat
     1921 aggggttgag gccctggcca agaagatctg cgacttcaag cagcagtaca cccagtacgg
     1981 cggtgtacgt cccttcggca ccgcccttct catcatcggc gcagaggagg acagggcccg
     2041 actctttgag accgacccct ctggagctct gctggagtac aaagccacag ggatcggagc
     2101 aggcagatcc tcagtgatgg aggtcttcga ggagaagtac agagaagacc tgaccatgga
     2161 cgaggcggtc ctcttgggcc tggaggctct tcacagagcc gcggagggca aggtggaggc
     2221 atcaaccact gagatcggca tcatcaagct ctctgataag ctattttata agttgacaga
     2281 aaaagaggtc agcgactacg ttgaacgcat gaaggcaata gtcgcttcca ataaggttga
     2341 accaggcaag ggagaagagg gagaggcata aggatggtca gactggatga cgcagtacct
     2401 gccaggctga agacccacgg aaccaccttt gaggttttgg tagacccgga cggagcgctt
     2461 gccctgaaga gaggcgatag cgtcaacctg gaagacatcc tggcggtgga ggatgtcttc
     2521 gagaatgcct ctcggggaga cagaagtccg gaagaagatc tgcaaaaggc cttcggaacc
     2581 accgatcccc tctccatcgc tcccatcatc atcaagaaag gagagatcag ccttaccgct
     2641 gatcagagga agaggttctt ggagaacaaa aggcggcagg tcattgaggt cattgccaga
     2701 aatgccatca atccccagac aaaaacccct catccaccag gccggatcga tcaggccatg
     2761 accgaggccc gcgtcaacat cgatcccacc aaatccactg acgagctggt caagatagtg
     2821 atgaaggcca ttcgccctct cattcccata agattcgagg aggtggaggt ggcggtcaag
     2881 gttccgcctg aatatgctcc caaggcctac ggagagatct ccgcctttgg cagactaact
     2941 cgggaatcct ggcagaacaa cggcgcctgg atcggcgtgg ttcagatacc cgcgggcatg
     3001 cagacggagt tttacgacct ggtcaaccgg ctaaccaaag gcgaggccga gacgaagctt
     3061 cttaagcatt aagaatggag taggaagcgc atggatcgca aggtggtagt accaggtgat
     3121 cttctctctg aggatgccaa gagatcgggc gaggggactt acatcaaaga tggaaacgtc
     3181 tactccttgc tttacggact tgcgaactac agggataaga taaatgtcat acctctggct
     3241 ggaaagtata tgcctgcgcc aggggataat gtcataggag tggtgaagga cataaccttc
     3301 tccaactgga tcgtggacat caattcgcca tatgacgggc ttttgcacat ctcggagttt
     3361 cccaagagga tcgattcgga tgagatgtcc aaatatctgc gcatcggcag ctcgattatg
     3421 acccgggtga aggatgtgga ccccacgatg aaggtggagc taaccttaaa cgaccgaaag
     3481 ctgggcccga tcaggacagg acaggtcata gagatcagcc atacccgggt acccaggctg
     3541 ataggcaagg gcggctccat gatcagcatg ctgaaaaaag aggtaaactg cagcatattc
     3601 gttggccaga acggcagaat ctggatcaac ggctgtgcag acgatacgga tctggcactc
     3661 aagacgattg ccctgataga gagagaagcc cataccaatg gcctgacgga caggattgtg
     3721 agcttcctta aggcagagaa agaggccaga agttgatcgc tatggatgag aatgatatat
     3781 cattttttaa agacggactc cgcctggacg gccggcgagc cgacgagctc aggccggtga
     3841 agatagaggt ggggatcctg gccagagctg atggctcgtg ttacatcgag atgggaggaa
     3901 ataaggtaat agctgcagta tatggaccca gagaggtcca ccccagacat ctgcaggagg
     3961 taacgagagc catcgtgcgc tatagataca acatggcatc cttctcagtg gaggagagaa
     4021 agcgtcccgg gccggatcga cggtcttatg agctctccaa ggtgagccgg gaggcgctgg
     4081 agccggtgat cctgacctca tttttccccc gttcggtgat cgacgtcttc gtggaggttc
     4141 ttcaggcgga tgcaggaacg aggaccgcgg gcataaatgc cgcggctgta gctcttgcgg
     4201 atgcgggaat tcccatgaag agcatgatat cctcctgtgc ggcaggaaag gtgggagata
     4261 ccattgtcct ggacccaatg aaagaggagg acaacttcgg acaggccgat ctgcccatcg
     4321 ccatgacccc caatggcgat atcaccctga tgcagatgga tggcaacctg acgccggacg
     4381 agttcggacg agcggtaatg atggccatga aaggggcaaa ggacatctat gagctgcaac
     4441 ggagggcttt agtggcaaag tatagccaaa tatccgcaga ctcagctcga gaggaggtgc
     4501 atctctgtga gtagcgtcat ctccgagatc aagaaggatt tcatatacaa tctccttatc
     4561 aaaggggaga ggatcgatgg caggaaattc gatcaatacc gggagatctc cattgagagg
     4621 gatgtcattc gcaaggctga gggaagcgct ctggtgaagc tgggatccag ccaggtcctg
     4681 gtaggagtga agatgcagcc cggagagccc ttccaggatt ctcccaaccg gggagtcata
     4741 atcaccaatg cagagcttgt gcccctggcc tcgccctcct tcgagcctgg gccgcccaat
     4801 gaggtgggaa tcgagctggc ccggttggtg gatcgggggg tgcgcgaatc gaaggcggtg
     4861 gaccttgaag ctctatgcat cgagccggga aagaaggtct ggatagtgtt catcgatgtg
     4921 catattctgg atgactgcgg aaacattctc gatgcagcct cgctgggggc aatagctgcc
     4981 cttttatgca ccaaggtacc tgcatccagg tttggcctgg gcgaggatta tatcctgccc
     5041 ataagggaca ttcccatagc cacgaccgcg attgaatttt ccgatgtgct gatgtttgat
     5101 cccggagtgg aagaggaggc gatagccaac accaagctca cagtcatcac cactacaaac
     5161 ggagatatct gcggaatgca gaagagcggc acaggaatgc tgaatccaga gcaggtctac
     5221 cgcatcattg atatagcatg tgagaaggcg aaagaaatcc gggaaaagtt tctggaagct
     5281 taaacggaag taaagatcat ggcaaagcag actacaaagg gaaagaagag cagatcggca
     5341 gccaggtttg gcgcgagata cggccgcaag tcgaggaagc tcgtggccga tctggaggag
     5401 aagagcaagg cctcttatga ttgcccccag tgtggacgga ataaggtaaa aaggacgagc
     5461 acagctatat ggagctgcag aaaatgtgga tataccttcg caggcgggtc ctttttgccc
     5521 gagacctctt taggccgctc tgtcttgcgg tctctcaaga agagccttga ggcggattaa
     5581 aggatggcct acaagtgtac cagatgcaag aagatcgtgg agattgatta cgagtacagc
     5641 ggcatacgct gtccttactg cggccatagg atcctgatga aggag
//
LOCUS       DAOSGP010000066         5594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_12829_length_5594_cov_21.388698, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000066 DAOSGP010000000
VERSION     DAOSGP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12829_length_5594_cov_21.388698"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..279
                     /locus_tag="PLV49_03110"
     CDS             49..279
                     /locus_tag="PLV49_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HOG97864.1"
                     /translation="MPILPVAPVSRLIREAGAKRVSEGARDALAEVLESYGLEVAREA
                     AGWANHAKRKTVKAEDIREAVKRVKPPAVLER"
     gene            283..2187
                     /gene="gatE"
                     /locus_tag="PLV49_03115"
     CDS             283..2187
                     /gene="gatE"
                     /locus_tag="PLV49_03115"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOG97865.1"
                     /translation="MATDNPPGLDYRQLGLICGIEIHQQLDTACKLFCGCPTAHREVE
                     DSNFEFFRYLRPSRSELGEIDRAALEEVLVSRKFHYKCYDTTCLVEADEEPPSEINPE
                     ALEISLVIARLLNMLIVDEVCTMRKMVIDGSNTSGFQRTAYIGSKGKIQTSQGPVGID
                     ILCLEEEAARIIEDRGDSLVYSLDRLGIPLVEIGTAPDIISPAHAREVASYLGMILRS
                     TGRVKRGLGTIRQDVNVSIKGGARVEIKGVQALNLVDKIVELEALRQHGLLQIKEELI
                     RRGAAVDDTVFDVTSLFSQTDSKVLARSIKNGGVVLACRLAGFAGLVGREVQPGRRLG
                     SEMSDRAKRAGVGGIFHSDELPAYGVTAAEVESVRAQLQAGKEYAVIMVTGPQDRARK
                     AMDAVLVRAREAMELVPEETRRALPDGCSEYMRPLPGSARMYPETDVPSVAITEQTLE
                     GLELPELFLARSERFGRDYGLNSELARVMAASPNYQLFEEIVSQIQLSPSIAVRTLET
                     IPIELARDGVDISALSDNHFKDALGLVAEGRIAKEGLAELFRALAERPDRSAADTAAA
                     AGLAGVDEAEVAKIVQAIVADKMDLVRSKGERAAAPLMGLVMKELRGKADGALVSAIL
                     NKEINNILSE"
     gene            2184..3317
                     /locus_tag="PLV49_03120"
     CDS             2184..3317
                     /locus_tag="PLV49_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HOG97866.1"
                     /translation="MNPERPKKPDMLLWEETLFKDRDIFELDHLPDQFLHRERQLDSL
                     KFCIRPALQGGRPVNALCLGPPGTGKTTAIFKLFEEIEAHSSRIVPVHVNCQMDSTRY
                     AVFYQLYKKIFEHAPPSSGISFKRVFEKVAQHSADEDKVLIVALDDINYLFPEKEVDH
                     VLYSLLRAHETCPGARMGVIGIMSELALNYVLDPRVVSVFQAEEVIFPLYSRNEIKDI
                     LSRRSQMGFYPGVIADDILETVVDHTEKSGDLRVGIDLLKRAGLNAEKRASKSISPED
                     VASSLERSRLVHLTYALKSLKADEKEVLRMATLQESWRAGELFRLFHEKTGAGYTRFH
                     EILNKLDAIRLINADFTGQGERGRSRIIRVRYEAEEILSRLNQ"
     gene            3433..4326
                     /gene="pstC"
                     /locus_tag="PLV49_03125"
     CDS             3433..4326
                     /gene="pstC"
                     /locus_tag="PLV49_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOG97867.1"
                     /translation="MMMPRRYSELFVETLLFVSAITSILVMLLIFYFLIHESIPAFSE
                     LGIISLLAGMKWHPSGDIYGFLPLITASLTITILALFINIAIGFPLAIYLAELAGPRS
                     KAILKPAIELLAGVPSIVYGFLGVIILVSYLQDSFDMLTGRSILAGSILLGVMFIPYL
                     TTICEDALRAVPGEFKEGSLALGANRWQTIRNVTIPAASSGITAAVLLNIGSIIGETM
                     AVLLVVGNVARIASPYYDIFDQGATFTSVIAGEMGEVARGSTHYHALFAVGLILLLVV
                     SILSLIADFARARIRKKFGGY"
     gene            4328..5575
                     /gene="pstA"
                     /locus_tag="PLV49_03130"
     CDS             4328..5575
                     /gene="pstA"
                     /locus_tag="PLV49_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOG97868.1"
                     /translation="MKLKPALAASVVISAFLFVSSLICLILQPELDRIKPIWEASLIL
                     CPLLILGSYLTMKGASVRTALLFILDILFCGSVFYLSYSGALNPDFIIKRPLGVGASF
                     EYSFLLPLLVLVASLLCIKDALNIVYGYTARGREALVFLGIRMSAMLSIILLGGILFI
                     IIYKGIWVINWEFLTSPHRRLGQAGGISTAILGSLWMVLGAMLISSPLGIGAAIYLHE
                     YSKSERLNRLITIAVSCLNGVPSVVYGLFGLAFLVSLFGVSLISGSIILGLINIPTII
                     LTSQEALKSVPNSLREGSMALGATKWQTIRKVVLPSAMPGILTGVIMGVAKASGETAP
                     IMWTAVTFTASPVQMIYGVIPDLFQPVNNLNYHLLNLIYFLGAWDVEARAWGTALVLI
                     SLVLSTNLLAIVVRNYYRKKISW"
BASE COUNT         1228 a         1441 c         1636 g         1289 t
ORIGIN      
        1 ttaaggctct tctggattca ttatccggat ttatggggtg tttgagatat gccaatactt
       61 cctgttgccc cggtgagcag actgattcgc gaagcagggg ctaaaagagt gagcgagggc
      121 gcaagagatg cgctggcaga ggttctggag agctatggcc tggaggtggc tcgtgaggca
      181 gcgggctggg cgaaccacgc caagaggaag actgtcaaag cggaggacat aagagaagca
      241 gtcaaaagag ttaaacctcc tgcggtttta gaaaggtgat cgatggcaac agataaccct
      301 cccggtttag actaccgcca attgggcctc atatgtggaa tcgagattca tcagcagctg
      361 gataccgcct gcaagctctt ttgcggctgc cccaccgctc atcgagaggt agaggactct
      421 aattttgagt tcttccgcta tctccgccca tcgagaagcg agcttgggga gatcgatcgc
      481 gctgccttgg aggaagtcct ggtctcgcgc aagttccatt acaagtgcta tgacaccaca
      541 tgcctggtgg aagcggacga ggagccgccg tccgagatca atcccgaggc cctggagata
      601 tctttagtca tcgcccgtct gctgaatatg ctgatcgtgg atgaggtatg caccatgagg
      661 aagatggtga tagacggctc caacacctct ggattccaac gcacggccta catcggctct
      721 aaagggaaga tccagaccag ccagggaccg gtgggcatag acatactctg cctggaggag
      781 gaggctgcga ggatcatcga ggaccgggga gacagcctgg tctactccct cgatcgcttg
      841 ggcattcctc tggtggagat tgggacggcg cctgatatca tctctcccgc tcatgcccgg
      901 gaggtggcct cgtacctggg catgattctg cgctccactg gccgggtgaa aaggggactg
      961 ggcaccattc gccaggatgt gaacgtctcc ataaaaggcg gggcccgggt ggagatcaaa
     1021 ggggtacagg ccctgaacct ggtggacaag attgtggaat tggaggcact ccgccagcat
     1081 gggctgctgc agatcaaaga ggagcttata aggcgggggg cagcagtcga tgataccgta
     1141 tttgatgtca catcgctctt ctcccagacc gattccaagg ttttagccag atccatcaag
     1201 aacggcggcg tggtcttagc ctgcaggctg gcgggatttg ccggattggt gggaagggag
     1261 gtccagccag gcagaaggct gggctcagag atgtccgatc gggccaagag agcgggggta
     1321 ggggggatct tccatagcga tgagctgccc gcttatggcg tgaccgctgc ggaggtcgag
     1381 tcggtaagag ctcaacttca agcagggaag gaatatgcag tgatcatggt cacaggaccg
     1441 caggatcggg ccagaaaggc catggatgcg gtcctggtcc gggcgaggga ggccatggaa
     1501 ttggtgccag aggagacgcg ccgcgctctt cccgatggct gctcggagta tatgcggcca
     1561 ctgccaggct cggcccggat gtatccggag acggatgtgc cctccgtggc gatcactgag
     1621 cagacgctgg aaggccttga gcttcccgag ctctttttgg cccgatcaga gcgctttggt
     1681 cgcgattatg gcttgaactc cgagctggcc agggtgatgg ccgcttctcc caactatcag
     1741 ctctttgagg agatcgtatc ccagatccag ctctcaccat ccattgcggt ccggaccctg
     1801 gagacgattc caatcgagct ggccagagat ggagtggaca tatcagcgct ctcagataac
     1861 catttcaagg acgccctggg gctggtggcg gagggacgga tcgccaagga agggctggcc
     1921 gagctatttc gcgcccttgc cgagcgtccg gacagatcgg cagctgatac tgccgctgct
     1981 gccgggctgg caggggtgga cgaggcggag gtggccaaga tcgtccaggc cattgtagcg
     2041 gataaaatgg atctagtgcg ttcaaaaggg gagagggcag ccgccccgct catgggcctg
     2101 gtgatgaagg agctgagagg caaggccgat ggcgccctgg tgagcgccat attgaataag
     2161 gaaataaata atatactgag cgagtgaacc cagaaagacc taaaaaaccg gatatgctcc
     2221 tctgggagga gaccctcttc aaggacaggg acatcttcga gctagatcac ctgcccgatc
     2281 agtttctcca tagagaaagg cagctggaca gcctcaaatt ttgcatccgg ccagctctgc
     2341 agggggggcg gccggtaaat gccctctgtc tcggcccgcc gggaacggga aagacaacgg
     2401 ccatcttcaa gctcttcgag gagatcgagg cccattcctc tcggatcgtc ccggtgcatg
     2461 tcaactgtca gatggactcc accagatatg cggtcttcta ccagctttac aagaagatct
     2521 tcgagcatgc ccctccatcc agcggcatct ccttcaagcg ggtcttcgag aaggtggccc
     2581 agcattcggc agatgaggat aaggtgctga tagtggccct ggacgacatc aactacctct
     2641 tcccagaaaa ggaggtggac catgtcctat actcccttct ccgggcgcac gagacctgtc
     2701 caggggccag gatgggagtg ataggcatca tgagcgagct ggctttgaac tatgttctcg
     2761 atcccagggt ggtctcggta tttcaggcgg aagaggtgat atttcctctt tacagccgca
     2821 atgagatcaa agatattctc agccgccggt cgcagatggg cttctatccg ggggtgattg
     2881 ccgatgatat cctggagact gttgtcgacc atacggagaa gtccggggat ctgagggtgg
     2941 gaatcgacct tctgaagagg gctggcctga atgcggaaaa acgcgcttct aagagcatat
     3001 cccctgagga cgtggccagc tcgctggagc gctcccgtct ggtccacctc acctatgcct
     3061 tgaaatctct gaaggcggat gagaaggagg tcttgcgcat ggcgaccctc caggagagct
     3121 ggcgcgcagg ggagctattc cgcctcttcc atgagaagac gggtgcggga tacacccgat
     3181 ttcatgaaat cctgaataag ctggatgcga tccggctaat caatgccgat tttaccgggc
     3241 agggtgaaag aggacgaagc cgcataatta gggtgcgcta tgaggctgag gagatcctca
     3301 gccgattgaa tcaatgaatt tttgaaaaaa gttggaccca tatgctccgt cctgagtaat
     3361 gcatctatat agatacaatg cataaggact ttaaccggtg gattcaattg accctcaatg
     3421 gatcggtcgc gaatgatgat gccccgcagg tatagcgagc ttttcgtaga gacgctttta
     3481 tttgtgtccg ccatcacatc gattctggtc atgcttttaa tattctattt tctcattcac
     3541 gagagcattc ccgcattttc ggagcttggc atcatctctc ttttagcggg catgaaatgg
     3601 catccttctg gtgatatcta tggctttctg ccccttatca ccgcatccct caccatcacc
     3661 attctggccc ttttcatcaa catcgccatc ggatttccac tggccatcta ccttgccgag
     3721 ctggctggcc cccggagcaa agcgatcctc aagccggcta tagagctcct ggcaggggtt
     3781 ccatccattg tctatggttt tttaggagtg atcatcctcg tatcctacct gcaggatagc
     3841 tttgatatgc tcaccggaag gtcaattctg gccggatcaa tacttctggg agtgatgttc
     3901 atcccttacc ttaccaccat ctgcgaggac gctctaaggg cggtgccggg ggagttcaaa
     3961 gaggggtccc tcgccctggg ggcgaaccgg tggcagacaa taagaaatgt caccataccc
     4021 gcggcatcat ccggaatcac cgctgccgtg ctcctcaata taggaagcat aataggcgag
     4081 accatggcag tgctgctggt ggtgggcaac gtggccagga tagcctctcc ctactatgat
     4141 atcttcgacc agggggcgac attcacctcc gtcattgccg gagagatggg tgaagttgcc
     4201 aggggatcga cccactatca cgccctcttt gccgtgggtc tcattctatt gcttgtggtc
     4261 tccatactga gcttgattgc ggattttgcc agggcgcgga tcagaaagaa gttcggagga
     4321 tattgagttg aagctgaagc ctgcactagc agcctcagtg gtcatatcag cattcttatt
     4381 cgtctcttcc ctaatctgcc tcatcctcca gccggaactc gatcggataa aaccgatttg
     4441 ggaggccagt ctcatcctct gtcctctctt aattctgggc tcatatctga caatgaaagg
     4501 cgccagcgta agaacagcgc ttttgttcat actggatatt cttttttgcg ggtcggtttt
     4561 ctacctgtcc tattcaggcg ctcttaatcc ggattttatc attaaaagac cgcttggtgt
     4621 cggggcatcc ttcgagtatt cattcctgct tcctctgctg gtcctggtcg ccagcctgct
     4681 atgcatcaag gatgccctca atatagttta tggctatacc gccaggggaa gagaggcgct
     4741 ggtgtttctg ggaatcagga tgtcggcgat gctctccatt atcctgctgg gcggaatcct
     4801 gttcatcatc atctacaaag gcatatgggt cataaactgg gagtttctga cttcacccca
     4861 caggaggttg gggcaggcgg ggggtatcag cactgccata ctgggatcct tatggatggt
     4921 tctgggggct atgctcatct cttcccctct tggaattgga gcggccatct acctgcatga
     4981 gtactccaag agcgagaggc tcaaccggct gatcaccatt gcggtgagtt gcttgaatgg
     5041 agttccctcc gtggtctatg gcctcttcgg actggcattt ctggtgagcc tgttcggagt
     5101 atcattgatc tccggatcga tcatcctggg ccttataaac atccccacca taattctgac
     5161 cagccaggag gcattaaaga gcgttcccaa ctctctgcga gaggggagta tggctttagg
     5221 agctacgaag tggcagacca taagaaaggt ggttttgccc tctgccatgc ccggcattct
     5281 caccggggtg atcatgggag tggccaaggc atcgggcgag actgcgccca tcatgtggac
     5341 ggcggttacc ttcactgcct ctccagtgca gatgatctat ggggtgatcc ccgatctatt
     5401 tcagccggtg aacaacttga actaccatct cctgaacctg atctatttcc tgggggcctg
     5461 ggatgtggag gctcgggcct ggggtacggc tctggtgctg atatcgctgg ttttaagcac
     5521 caaccttctg gccattgtgg tgagaaatta ctaccgcaag aagatctcct ggtaaatggc
     5581 tcatgagaca aaaa
//
LOCUS       DAOSGP010000067         5542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13005_length_5542_cov_12.539275, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000067 DAOSGP010000000
VERSION     DAOSGP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13005_length_5542_cov_12.539275"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1296
                     /locus_tag="PLV49_03135"
     CDS             52..1296
                     /locus_tag="PLV49_03135"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HOG97869.1"
                     /translation="MSNALSIVAVTDTLRNLLLYAINADDELAGAQVTTSPLDKARGG
                     GTPNQLNLFLYQMAINPAWCNMVIPARVKGGESGFPPLALDLYYLVTAYGSKDDETLS
                     HRLLGQAMRCLHDHTTLETEELRNALPGNDLHAQGERVRITLQPLSLEEMSKLWTTFQ
                     TQYRLSAAYKVSVVLIESTKDAKSALPVLTRGKEDRGAAVLGSLYPSLTEAVPPAPMP
                     SLRLGQDLEIRGNNLDKNVVKVRISGRHLSQPIELDPLSGGTSEALKVHIKSESEDDG
                     ALARWAPGFYTVSLMIDLPEIAHWATNEVPFALAPDIAIETIPKDPPPGDLALSVTCR
                     PRIREDQRVTLLFGSQQISVASVTATNASEPTNLKFFIPDVKVGSYVVRLRVDGVDSP
                     SAEISELTSRLEFKEGHKVIVA"
     gene            1293..3569
                     /locus_tag="PLV49_03140"
     CDS             1293..3569
                     /locus_tag="PLV49_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG97870.1"
                     /translation="MIPADWQQKNDEYLALALAWLRLRLELQAQPESERERETKASQE
                     EAYPAPAGQQSDGSPEKKAWGQSEENKAQSSATSTERQGWIKKLLTGELSSRETSADS
                     QKFDLEPNATGALHAEREDLIDKPQSPAKKVDVAQAAINMEEAGKVEPPPALIMLSQR
                     LGLSRFEQDLLLLSAAMELDTRIEGLCARAQDDMARPYPTFALGLALFDNPSWDALSP
                     HRPLRYWQLLEIHQAAAQPLTSSPLRADERIVSFIKGLNYLDDRLSSILVPLPLSNGK
                     ADLPPSQSASVDEAVQHLGQGAERGRFPVIQLLGWDRQSMRLMAGMTASRLGLHLYRL
                     PAELLPSDVTELETLARLWQRESLLLPVALYLDAQNLDRNRSSEAASAASAVDRFLGR
                     LSCIVFLDVGETWQFSWGEGIGIEVSKPTPQEQKAAWAAALGKEENGTADLLAGQFNL
                     SLAGIGQVISGAKEDQSAVPLKDRLWKGCLALTRPRLDVLAQRIDARAGWDDIVLPAA
                     EASLLHQIADQVRHRSQVYDRWGFRREMNRGLGISALFAGESGTGKTMAAEVIANDLG
                     LSLYRIDLSAVVNKYIGETEKNLRWLFDAAEDGGAILFFDEADALFGRRSEVKDSHDR
                     YANIEINYLLQRIESYRGLAILATNMKSALDLAFLRRLRFIVNFAFPGQTERKLIWQK
                     VFPEETPLQALNYDKLASLNLTGGSIHNIALSSAFLAASAGSQVTMPIVLSAARTEMR
                     KLDRPINEADYVWTGGAG"
     gene            3566..3829
                     /locus_tag="PLV49_03145"
     CDS             3566..3829
                     /locus_tag="PLV49_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97871.1"
                     /translation="MSIHLHIDRLVLEGLPLSPTDGPLLQAALESELTRLMGDGLSPE
                     LAEGAAVPRLKAKGIEVAPSDGPEAIGRKIAGSVYGGLGDGRA"
     gene            3816..>5542
                     /locus_tag="PLV49_03150"
     CDS             3816..>5542
                     /locus_tag="PLV49_03150"
                     /inference="COORDINATES: protein motif:HMM:NF026945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HOG97872.1"
                     /translation="MEGLKAASISKQSTPSSGVMQRKCSSCKKKEKEKVRLQRTAVRS
                     GPVGEVPPIVHEVLRSPGKPLDKATREFMESRFSHDFTHVSPQAAMQTTGMSIGPQGD
                     RYEQEADRAAEAAMKQATPQRAHSDPGYDFSNVRVHDDSRAAESARAVNALAYTVEQD
                     IVFGSGYSPNTDLGKKLLAHELAHTLQQSSSDGALRRKCGAAAMGPASPDCQIDTNAR
                     PRSNRLGEDRFLFKKNCDDFDADHESRLSEVASRIPANARVSVLGMASRDGPADFNRS
                     LSCERAFRGAEIIIRSGHGSQIDDVRATGPIGAEGDTHLRAVEVLVTAPRQPQRQPKP
                     RCGPDATDWFIRTINGARRDRDIIGIRRDMADADALARRYGATAADVAEGGAAAAVLA
                     QEQKLRISGPAPPPRNATIDAQLAAGISRGASATASLTPRPPLPFLPADGRLIDTPRI
                     LFHINRAANNWKALVTHGARYDFKAHVMNHPRSAHCPEPGCIPIEVGTITLCPAATSE
                     NCYESDLPGNLFYARIGRHVGYSRNTLQLGSQYAELTDTRLRPARPAVTWDTPEDTFS
                     IDLGFSLPLP"
BASE COUNT         1306 a         1486 c         1606 g         1144 t
ORIGIN      
        1 tactatgagc ctgaatgaat acttgcgcca gcgtgcagga ggagatggaa agtgagcaac
       61 gctctttcaa tagtagccgt tactgacaca ttaagaaatt tgctcctcta tgcgatcaat
      121 gccgatgatg aactggccgg agctcaagta acaacctcgc ccctggacaa ggcgcgagga
      181 gggggcactc ccaatcagct caacctattt ctctatcaga tggccataaa tcccgcctgg
      241 tgcaatatgg ttattcctgc ccgggtcaag ggcggagagt ccggctttcc ccctctggca
      301 ttggatctat actacctcgt tactgcttac ggaagcaaag atgatgagac cttgagccac
      361 cgccttctcg gtcaggccat gaggtgcctt catgatcata ccactctgga aaccgaagag
      421 ttgcgaaatg ctctgccggg aaacgatctc catgcacaag gagaacgagt gcgaatcacc
      481 cttcaacctc tctctttgga ggagatgtcc aagctctgga ccaccttcca aacccagtat
      541 cgcctctcag ctgcttacaa agtatccgtg gtgctcatag agagcacgaa ggatgccaaa
      601 tcagctctgc cggtgctgac ccgaggaaag gaagatcgag gagctgcagt cctggggtct
      661 ctatatccct ccctcacaga ggcggttcca ccagcaccta tgcccagcct cagattgggg
      721 caagacctgg agatcagggg aaataacctg gataagaatg tcgtaaaggt aaggatcagt
      781 ggccggcatc tatctcagcc tattgaactg gatccccttt cgggagggac atctgaggcg
      841 ctcaaggttc acattaaaag tgagagcgaa gatgatggtg cacttgccag gtgggctccc
      901 ggcttctata ctgtgtcact gatgatcgat ctccctgaga ttgcccattg ggccactaac
      961 gaggtgccct tcgccctggc acctgacatt gccatcgaga ccattcccaa agatcctccg
     1021 cccggggatc tagcactcag tgtcacctgc agacccagga tcagggagga tcagagggtg
     1081 acactgctct ttggatccca acagatatct gtagcatcag tgacggcgac gaacgcttct
     1141 gagccaacaa atctcaagtt cttcatcccc gatgtcaaag taggatctta cgtcgtcaga
     1201 ctgcgcgtcg atggagtgga tagcccgtcc gcagagatct cggaattgac atccagattg
     1261 gagttcaaag aaggccataa ggtgattgtg gcatgatacc tgcggactgg cagcagaaga
     1321 acgatgaata tctcgctctg gcccttgcct ggctgcgcct gcgtctggaa ttgcaggccc
     1381 agccggagtc tgagagggag agagagacca aagcgtccca ggaggaggcc tatcctgctc
     1441 ctgccggtca gcaatctgat ggtagtcctg aaaagaaggc ttggggtcaa tctgaagaga
     1501 acaaagccca aagctctgcc acatctacgg aaagacaggg ttggattaag aagctattga
     1561 cgggcgaact ctcttccagg gagacttcgg ctgactctca gaagtttgat cttgaaccca
     1621 atgcgactgg agctcttcat gcagagcggg aagatctcat agataagcct cagtcaccag
     1681 cgaagaaggt ggatgtcgct caggcggcca taaatatgga agaggcagga aaggtcgagc
     1741 cacctcccgc tctgataatg ctcagccagc gtctgggcct atctcgcttt gaacaggatc
     1801 tgctcctcct ctctgcagcc atggagcttg acacgcggat cgaagggcta tgcgccaggg
     1861 ctcaagacga tatggcaagg ccctatccaa ccttcgccct ggggcttgca ctctttgata
     1921 atccatcctg ggatgcgctc tcgcctcata gaccgcttcg ttactggcag cttctggaga
     1981 ttcaccaggc tgcggcccag cctctcacct ccagtcccct tcgagcggat gagagaatag
     2041 tgagtttcat caagggcctg aattacctgg acgaccggct gagtagcatt ctcgtcccac
     2101 tgcccctctc gaatgggaag gcggatcttc ccccttctca gtctgcttcc gtcgatgaag
     2161 cagtgcagca tcttggccag ggtgcagaaa gaggacgatt tcccgtaatc cagctcctcg
     2221 gatgggacag gcagagcatg cggctcatgg cagggatgac cgcctccagg cttggccttc
     2281 acctctatcg tctgcccgcc gagctgctgc cttctgatgt cacagaactt gagactttgg
     2341 ccaggctatg gcagagggag agcctattgc taccggttgc tctttacctg gatgctcaga
     2401 acctggatcg aaatagaagc agtgaagcgg cttcggctgc gtcggcagtc gatagattct
     2461 tggggcgtct gagctgcatc gtgtttttgg atgttggaga gacctggcag ttctcctggg
     2521 gggagggaat cggaattgaa gtctccaagc ccacacccca ggagcaaaag gccgcctggg
     2581 ctgctgcttt gggaaaagag gagaatggaa cagcggattt gttggcaggc cagttcaacc
     2641 tcagtctggc gggaataggt caggtgatct caggggcaaa ggaggaccaa tcggccgtgc
     2701 cgctcaaaga tcggctctgg aagggctgcc tggccctcac caggcctcga ctggatgtgc
     2761 tggcgcagcg catcgatgct cgggcaggat gggatgatat agtccttcct gccgccgagg
     2821 cgagtctcct gcaccagata gcagatcagg ttcgccatcg cagtcaggtc tatgatcgct
     2881 gggggtttcg cagggagatg aaccggggac ttgggatcag cgccctcttt gcaggggaga
     2941 gcggaaccgg caagaccatg gccgcggagg tcatagccaa tgatctcgga ctgagcctct
     3001 accggattga cctctcagct gtggtcaaca agtacatcgg cgagactgag aagaatctgc
     3061 gctggctctt tgatgcggct gaggacgggg gagccatcct cttctttgac gaggccgatg
     3121 ccctcttcgg gcgcaggagt gaggtgaagg acagccacga ccgctatgca aacatagaga
     3181 ttaattatct tctgcagcgc attgagtctt accggggact ggccatcttg gccaccaata
     3241 tgaaaagcgc cctggatttg gcgttcctgc ggcggctgcg tttcattgtc aacttcgctt
     3301 ttcccgggca gactgagcgc aagctgatct ggcagaaggt ctttccagaa gagacgcccc
     3361 tgcaggccct gaactatgat aagctggcca gcctcaatct cactggtggc agcattcaca
     3421 atatcgcttt gagctctgca ttcctcgcgg cctccgcggg ctcgcaggtg accatgccca
     3481 tagttctctc tgctgcccgc acagagatgc gcaagctcga ccggcccata aatgaagcgg
     3541 actatgtctg gacaggaggc gctggatgag cattcatctt catatcgacc ggctggtgct
     3601 ggaaggtctg ccgctctcgc cgaccgatgg tccgctgctc caggctgccc tggagtcgga
     3661 gctgacgagg ctgatgggcg atggactatc accagagctg gcagaaggtg cggccgtccc
     3721 cagattgaag gctaaaggca tagaggttgc accatcggat gggcccgagg ctatcggccg
     3781 gaagatagcc ggatcggttt atggaggact gggagatgga agggcttaag gctgccagca
     3841 tttccaagca gagcacgccg tcatcaggcg tcatgcagcg caagtgcagc agctgcaaga
     3901 agaaggagaa agagaaggtt cgcctgcagc gaactgcggt gagatctggc ccggtggggg
     3961 aggtgccgcc cattgtgcac gaggtgctga gatcgccggg gaagccacta gataaagcga
     4021 cgcgggagtt catggaatcg agattctcgc atgacttcac tcatgtcagt ccgcaggccg
     4081 ccatgcaaac gaccggcatg tcaatcggtc cgcaaggcga cagatacgaa caggaggcag
     4141 acagggccgc agaagcggca atgaagcaag cgaccccgca aagagcccat tcggatccgg
     4201 gatatgactt tagcaatgtg agagtgcacg atgattcaag ggccgcggaa tcggcgcggg
     4261 ctgtgaatgc attggcgtac accgtggagc aggacatagt attcggatcc ggatattctc
     4321 caaatacgga tctgggaaag aagcttcttg cacatgagct ggctcatacg ctgcagcaaa
     4381 gcagcagcga tggtgctctt cgaagaaaat gcggcgcagc ggcgatgggg ccggcaagcc
     4441 ctgactgcca gatcgacaca aacgcaaggc caagaagcaa tagattaggt gaagatcgtt
     4501 ttctattcaa gaagaactgt gatgattttg atgctgacca cgaatcgcgg ctctccgaag
     4561 ttgccagcag aattcctgcc aatgccaggg ttagtgtcct gggcatggcc agcagggatg
     4621 gaccggcaga tttcaataga agcctttcat gcgagcgtgc cttcaggggc gcagagatca
     4681 ttatacgaag cggtcatggc agccagatcg atgatgttcg agccacgggc ccgataggtg
     4741 cagaaggcga tacccatctt cgtgcggttg aggtcctggt cactgctcct aggcagcctc
     4801 agagacagcc caagccgaga tgtggaccgg atgccacaga ttggttcata agaaccataa
     4861 atggagccag gagagaccgg gacatcattg gcatccgaag agatatggca gatgccgatg
     4921 cactggccag gcgctatggt gcaactgctg cagatgtggc tgaaggcgga gcggctgcag
     4981 cagtccttgc acaggaacag aaattgagaa tatctggacc ggcgcctcca ccacgcaacg
     5041 ccaccattga tgcccagctt gccgcaggca tatccagagg agcttctgcc acagcaagcc
     5101 ttaccccgag accaccattg cccttcctgc ctgcagatgg acgcttgatc gatacccctc
     5161 gcatactttt ccatattaac agggcagcca ataactggaa agcattggta acccacggag
     5221 cgagatatga tttcaaggct catgtcatga atcaccccag aagcgcccat tgtcccgaac
     5281 caggttgcat acctattgag gttggaacca taaccttatg ccctgcagcg acctctgaga
     5341 attgctatga atcagacctg cccggaaatc tgttctatgc taggatagga aggcatgttg
     5401 gctacagcag aaatacgctg cagcttggct cacaatatgc ggagctgact gatacaaggc
     5461 tgcggcctgc gcgtccggcc gtgacctggg atacgcctga ggatacgttc tcaatcgatc
     5521 ttggctttag cctgcccttg cc
//
LOCUS       DAOSGP010000068         5512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13078_length_5512_cov_19.103353, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000068 DAOSGP010000000
VERSION     DAOSGP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13078_length_5512_cov_19.103353"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLV49_03155"
     CDS             complement(<1..701)
                     /locus_tag="PLV49_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG97873.1"
                     /translation="MDAYNILWADLVTLKRHWSRYLITTLMSPLLYLVAFGWGLGRSI
                     NLNGTSYLEFVIPGIIALTAMSTSFNGASTRLNVDRLYYKSFDECLMAPIGLPSMLMG
                     KAMIGVVRGLMSSFVFIAVALLIAPTIHIGPPFLVGLLLTCLTFAFLGVLAALLARSH
                     EDMATFASLILMPMTFLGGTFFSLSQVPTGLKYALYALPLTHSSLFLRAAALDQPLPW
                     ISLFILLVFFAAFLAG"
     gene            complement(703..1554)
                     /locus_tag="PLV49_03160"
     CDS             complement(703..1554)
                     /locus_tag="PLV49_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG97874.1"
                     /translation="MIEISNLIKEYSGKRVVDIQSLQVDPGELFGFLGPNGAGKTTTI
                     RILTTLTKPTSGRALINGSDVVRDLSKVKLEFGIVQQHLSLNKDLTIAENLELHARLH
                     HLPKAQRTKRISELLDYVELSEHADYLIDDVSGGMKRRAMIARALIHRPKLLFLDEPT
                     VGLDAQTRRKVWDLIRKMNSDGTTVFLTTHYIEEAEALCERVGILHKGKMIAIGSPLG
                     LRQKLGMVAVEMQTNGNGTQYRYFPDRSLAAQFVQGLPGTNKLAMRESNLEDVFVELT
                     GQKVMES"
     gene            complement(1577..3619)
                     /locus_tag="PLV49_03165"
     CDS             complement(1577..3619)
                     /locus_tag="PLV49_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative cobaltochelatase"
                     /protein_id="HOG97875.1"
                     /translation="MHHIKRRTIPFTSIVGQEDMKFALILNAINPRIGGVLIRGDKGT
                     AKSTAVRALADLLEDIVVVEDCPFNCNPRNVEEMCDLCFDRSQRGQIKEASRKTPVID
                     LPLGATEDRVVGSLNVERAIKEGIRALEPGILAAANRGILYIDEVNLLDDHVADVLLD
                     SAAMGVNIVEREGVSVAHPSKFILVGTMNPEEGELRPQLLDRFGLQVNVVGIEDIDQR
                     MLIAKTAERFDADPEGFSKEQQALQDGLKGKISAAREILGRVTMSDDLLRTIASTCID
                     LGVKTHRAEIVISRTAKTIAAFEGRTEANQEDIKKAMELALAHRMRSKPFEPPTLNKD
                     KLEKSMEKQQQDQQQQKQEHEQQPKQPPQQKKQEQQPESSDQQEDSNQAQAASPQEQI
                     FEIGAPIDVRQINMPRKRDKFFRRKTSGRRINTLALQNSGRYLRQRMPKEGKDIAIDA
                     TIRAAAPYQKARSGPNAIKVKSEDIREKERVRKTSAMLLFVVDGSGSMGAMQRMESAK
                     GAVLSLLMDSYQKRDKIGMVAFRGKEAELILPPSSSVDLALSRLKELPTGGKTPLSAG
                     LSRGLQLLQGEMKKDAETKLMMVLVSDGRANVGMGGKIKDELMEISERTKQLGVHTIV
                     IDTEVVDSSFMEMRLGYCREIAEMTGGKYYPISGLSSEALYSIVDGEQKLLLEANS"
     gene            complement(3629..4411)
                     /locus_tag="PLV49_03170"
     CDS             complement(3629..4411)
                     /locus_tag="PLV49_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG97876.1"
                     /translation="MVNITIKSLTFGYNGSMILDGLNLVVEDSEVLGLVGPNGSGKTT
                     LIKCIDKILKPKGSILIDGRDIDTVSRTDLAKRLGYVPQSSSTPLATTVFDTVLMGRR
                     PHISWRVSDSDLDKVADILGLLHLEYLAMRDFSQLSGGQKQKVLIARALAQEPEVLLL
                     DEPTSSLDMKHQLEVMETISSLVKEKKISAVMALHDLNLASMFVDKLAILKGGKIYAA
                     GEPIDLLNAKNIRDVYGVEAVVMNNLDRPYIVPLRSLNEGVA"
     gene            complement(4405..5466)
                     /locus_tag="PLV49_03175"
     CDS             complement(4405..5466)
                     /locus_tag="PLV49_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG97877.1"
                     /translation="MSLSKTEVMGEYQSYMGKRALFFLILLLGTALLASMAVTLGSAS
                     LSIKEVYVAIMARFLPDYFESTSYANTIVWGLRLDRILMGILSGMGLAIAGAAMQGIL
                     KNPLASEFTLGVSSAAGFGAALAIVLGAGFVGGEYLVIGNAFIFTLIASFTVYGLAKY
                     KGITPETLILAGIAIMYLFSAMTSFLQYVGHAEQVQEVVFWMMGSLGKSSWEKAGIIS
                     AILAVCLPYLIIKSWDINAMGAGDETAKSLGVNVERTRVISMVLVSLITASIICFTGT
                     IGFVGLVAPHITRMVIGGDHRFLLPGSALVGALLLLGADTLARTILAPVILPVGIMTS
                     FLGVPFFVYLFMKRRKVFW"
BASE COUNT         1258 a         1548 c         1336 g         1370 t
ORIGIN      
        1 cccgccagaa acgctgcaaa gaagaccaga agtataaaca aagagatcca gggcaggggc
       61 tggtccaggg ctgcagctcg cagaaacagg ctggagtgag tgagcggcag agcatagagg
      121 gcatacttca gcccggttgg gacctgggat agagagaaga aggttccgcc cagaaaggtc
      181 atgggcatca agatcaagct tgcgaaggta gccatgtcct catgagacct cgccagcaga
      241 gccgccagaa ctcccagaaa ggcgaatgta aggcaggtca gaagaagacc aaccagaaaa
      301 ggcggaccga tgtgaatggt aggtgcgatg agcaaggcta cagcgataaa tacgaaggag
      361 ctcattaagc ccctcaccac cccgatcatc gcctttccca tgagcatgga gggcagtccg
      421 attggggcca tgagacattc atcaaagctc ttgtagtaga gacgatcgac gttgagcctc
      481 gtgcttgcgc cgttgaagct ggtgctcatg gcggtgagag cgatgattcc cggtatcacg
      541 aactcgagat agcttgttcc attgagattt atgcttcgcc ccagtcccca gccaaatgcc
      601 accagataaa gcagtgggct catcaaagtg gtgatgaggt atctggacca gtggcgcttc
      661 agggtcacaa ggtccgccca aagtatgtta taagcatcca tattaactct ccatgacctt
      721 ctgaccggtc agttcaacaa agacatcctc caggttcgac tctcgcatgg ccagcttatt
      781 ggttcccggc aatccctgaa cgaactgggc agcgagcgac ctatcgggaa aatatctata
      841 ctgcgtgccg tttccattag tctgcatctc gactgctacc attcccagct tctggcggag
      901 acccaaagga gacccaatgg caatcatctt tcctttatga aggatgccca ctctttcgca
      961 taaggcctca gcctcctcga tatagtgggt ggttaagaag accgttgtac catctgagtt
     1021 catttttcga atgagatccc agacctttcg acgggtttgt gcatccaatc ccaccgtcgg
     1081 ctcatccaga aatagcagct tcggcctgtg gataagagcc ctggcgatca tggctctcct
     1141 cttcatgcca cctgagacat catctatgag atagtctgcg tgctcggaca gctccacata
     1201 atcgagcagt tcagatatcc ttttagttct ctgtgctttg ggcagatgat gaagccttgc
     1261 atgaagctcc aggttctcag cgattgtcag gtctttgttc aggctcaggt gctgctgaac
     1321 tatgccgaat tccagcttta ccttggatag gtccctgacc acatcagagc cgttgatgag
     1381 cgctctcccg gaggtgggtt ttgtaagcgt ggtgagaatt ctgattgtag tagtctttcc
     1441 ggcaccattc ggccccagaa atccgaagag ctctccggga tcaacctgaa ggctttggat
     1501 atcaactacc ctttttccgg agtactcttt aattagattc gatatttcta tcatcccaat
     1561 cacaacgccc aaaagatcaa gagttggcct cgaggagcag cttctgctct ccatccacaa
     1621 tgctgtaaag cgcctcggag ctcaagcctg atatcggata atattttccg ccggtcatct
     1681 ctgcgatctc ccggcagtag ccgagcctca tctccatgaa tgatgaatcc accacctcgg
     1741 tatctatgac aatggtgtga actccgagct gctttgtcct ttccgagatc tccatcagct
     1801 catctttgat ctttccgccc attcccacat tggccctgcc atcggatacg aggaccatca
     1861 tgagctttgt ctctgcatcc tttttcatct ctccctgcaa cagctgcaat ccgcgggaca
     1921 gacctgcaga aagcggcgtc ttgccgccag tgggtagctc ctttagcctg cttaaggcca
     1981 gatccacgct ggaagaggga ggaagtatca gctctgcctc ctttccccta aaggccacca
     2041 ttccaatctt gtcccttttc tggtaggagt ccatgagcaa ggagaggact gctcccttgg
     2101 cgctttccat cctctgcatg gcacccattg agccactgcc atctaccaca aagagcagca
     2161 tggccgacgt ctttcgcacc cgctccttct ctcgaatgtc ctcgctcttc actttaatgg
     2221 cattgggtcc tgatctggcc ttttgatatg gagctgcagc acgaatggtg gcatcgatgg
     2281 cgatatcctt tccctcttta ggcatccttt gtctcaggta ccttccggag ttttgcagtg
     2341 caagggtgtt tatcctcctg ccactggttt ttctgcgaaa aaatttgtcc ctctttcgag
     2401 gcatattgat ctgccgcaca tctataggcg ctccaatctc gaagatctgc tcttgcggtg
     2461 atgcagcctg agcttgatta gagtcctcct gttgatcgga actctcgggc tgttgctcct
     2521 gttttttctg ctggggaggc tgctttggct gctgctcatg ctcttgcttt tgttgctgct
     2581 ggtcttgctg ctgcttttcc atagactttt ccagcttatc tttgttgagg gtcggcggct
     2641 caaaaggctt gctcctcatc ctgtgagcca gcgccagctc catggccttc tttatatcct
     2701 cctggttcgc ctccgtcctt ccttcaaaag cagctatcgt cttggccgtt ctggagatca
     2761 ctatctctgc cctgtgggtc ttaaccccca ggtcgatgca ggtagaggct atggttctca
     2821 gaagatcatc gctcatagtc accctgccaa ggatctccct ggcagcagag atcttcccct
     2881 taagaccatc ctgaagagcc tgctgctctt ttgagaagcc ctccgggtcg gcatcaaacc
     2941 gttccgccgt cttggcaatc agcattctct gatctatatc ctcgatgccg acaacattta
     3001 cctgcagccc gaatctgtcc agcagctgcg gtcttagctc tccctcctcg ggattcatgg
     3061 tgcccacaag gataaactta gacggatggg caacggacac gccctcgcgt tccacgatgt
     3121 tcacccccat agcagcggag tccagcagca cgtctgccac gtgatcatcc aggagattca
     3181 cctcgtcgat gtacagtata cccctgttgg cagcagccaa aattcctggc tccagggccc
     3241 ggattccctc tttgattgcc ctttctacat tcaacgatcc cacaacccga tcctctgttg
     3301 cacccagggg aaggtcgatc accggggtct tgcgtgaagc ttctttaatc tggcctctct
     3361 gactcctgtc aaagcaaagg tcgcacatct cctccacatt tctgggattg cagttgaaag
     3421 ggcagtcctc taccacaaca atatcctcaa ggaggtcagc tagggctcgc acagcggttg
     3481 atttagcggt tcctttatca cctcttatca gcacgccgcc tatgcgcgga ttgatcgcat
     3541 tcaggattaa tgcaaacttc atgtcctcct ggcccacaat ggatgtaaaa ggaatggtcc
     3601 ttcttttgat atgatgcatt tcttcacctt atgctacgcc ttcgttaagt gatcgcaaag
     3661 gaacgatgta cggcctgtcg aggttattca tcaccacggc ctcgactccg tacacatccc
     3721 ggatattctt ggcatttaaa agatcaatag gttctcctgc tgcgtaaatc tttccgcctt
     3781 tcaggatcgc cagcttgtcc acgaacatgg aggccagatt cagatcatga agtgccatga
     3841 ccgcagagat cttcttctct ttgaccaggg acgatatcgt ctccatgacc tcgagctggt
     3901 gtttcatgtc cagacttgag gtgggctcat ccagtagcag cacctccggc tcctgggcca
     3961 gagccctggc aatcaggacc ttctgcttct gacccccgga tagctgggag aagtccctca
     4021 tggccagata ttccaaatgc aaaagcccca ggatatctgc taccttatcg agatcggagt
     4081 ctgatacgcg ccagcttata tgcggcctcc tgcccatgag aactgtatca aaaacagtag
     4141 tggccagagg agtcgagctg gactgaggaa cataccccag gcgtttggcg agatctgttc
     4201 tgctcacagt atcgatatct ctgccgtcta tcagaatgct tcctttgggc ttgagtatct
     4261 tatctatgca cttgatcagg gttgtcttgc ccgagccgtt tgggccgacc agccccaaca
     4321 cttctgaatc ctcgaccacc agattgaggc cgtccaggat catggagcca ttgtagccga
     4381 atgtcaggct ctttatagtg atatttacca aaacaccttc ctccttttca tgaagagata
     4441 aacgaagaac gggactccca ggaatgacgt cattatgccc acaggaagta ttaccggtgc
     4501 gaggatcgtc ctggcaaggg tgtctgcacc taaaagaagc aaagcgccca ccagggctga
     4561 gcccggcaga agaaaccggt gatctcctcc aattaccatc ctggtgatat gaggagccac
     4621 caatcccaca aagccgatgg ttccagtaaa gcagattatg ctggcagtta tgagcgagac
     4681 cagcaccatg ctgataacgc gtgtcctctc gacgttcacg cccaggctct tggcagtctc
     4741 gtcacctgcc cccatggcat tgatgtccca tgacttgatg atcagataag gtagacagac
     4801 tgccagaatt gctgaaatta ttccggcctt ttcccaggag gatttgccca gggagcccat
     4861 catccaaaaa acgacctcct gcacctgttc tgcatgtcca acatactgca gaaacgatgt
     4921 catggcactg aacaggtaca tgatggcgat tcctgccagg atcagagttt ctggagttat
     4981 gcctttgtac ttggcaaggc cgtagacggt gaaggatgcg atcaatgtga agatgaacgc
     5041 attgccgatt accaggtact ctcctcccac aaatccggca ccgagcacta tggccagggc
     5101 tgcaccgaag ccggctgctg aagatacgcc cagggtgaat tcgctggcaa gaggattttt
     5161 caagatgccc tgcatggctg cgcctgcgat cgccaatccc atgcctgaca gtatgcccat
     5221 gaggatccga tcgaggcgga ggccccagac gatggtgttt gcatatgacg ttgattcaaa
     5281 gtagtcggga agaaacctgg ccatgatggc aacatagacc tccttaatgc tgagacttgc
     5341 agaaccgagg gtgacggcca tgctcgccag caatgcggtc cccagcagca gtatcaggaa
     5401 gaagagcgcc ctcttgccca tatagctctg gtattcgccc atcacctcag tctttgatag
     5461 agacataggc ctccagaacg atgcccaagc agatcaaaac ccagaatttg tt
//
LOCUS       DAOSGP010000069         5505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13095_length_5505_cov_18.363119, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000069 DAOSGP010000000
VERSION     DAOSGP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13095_length_5505_cov_18.363119"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLV49_03180"
     CDS             complement(<1..392)
                     /locus_tag="PLV49_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HOG97878.1"
                     /translation="MKEKWLMAKGSWEEIKPQITTALESGFECVIVDRENVERVRELG
                     KIQVACFGAERDAEDILVIGRHGEGDGSVPLPKDMNSSMDYALAKTISGNKAAYVVIA
                     GKKYEQFAVEMGKHVDYLLVIGTDWKVIP"
     gene            486..1187
                     /locus_tag="PLV49_03185"
     CDS             486..1187
                     /locus_tag="PLV49_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HOG97879.1"
                     /translation="MENRLIYSPVGILIMLGFAFLIAVVVSFLFLDLARTAFTKIGFS
                     WSQALFVLLASLVGSSINIPLTNLECSTPMVHERHIRAFGVSYHVPVVKSCNTLLAIN
                     VGGALIPSLISMALIYRFPESIYYALSGIVLVAIITNRVARPVRGLGIVTPALVPPLC
                     AALAAILMVYVLGAPHDLIFLIAYAAGTLGTLLGADILNLGKIRDLGAPVASIGGAGT
                     FDGVFLSGLIAVLLV"
     gene            1295..>2496
                     /locus_tag="PLV49_03190"
     CDS             1295..>2496
                     /locus_tag="PLV49_03190"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOG97880.1"
                     /translation="MSFRGFLDDLEGSGLMNHVRDPVSPVQEVTERSWGSGPILFDDV
                     SGHKCCLNILGTRDLLARALGIPAEDMVRHLSQIGCQGPVREVDWSSFMENVSQPDLS
                     RLPIMTYFPGDGGPYITSGVVVSRFEDKINACVHRLMVLGKDRLAARLVPGRHTHQLY
                     EAALAKGKEVAVAVAIGVDPLVLMAASTRVPPEMEFCYAAALRGSHVELIALDNGVLI
                     PHAQIVLEGYITTERAPEGPFVDITGTRDLVRQEPVIRLTRMMMCNEPIYHGLLPAGG
                     EHKMLMGVPYEPLIYREVSKVAKVKDVMLTEGGCCYFHAVVQIEKETEEDARRAIDAA
                     IKAHRSLKHVLVVDSDINIHDPRDLEYALATRMRGDEDIVLYPNVRGSTLDPRSNEGM
                     TTKVGVDAT"
     assembly_gap    2497..2596
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2746..3828)
                     /gene="ftsZ"
                     /locus_tag="PLV49_03195"
     CDS             complement(2746..3828)
                     /gene="ftsZ"
                     /locus_tag="PLV49_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG97881.1"
                     /translation="MELDIREAMDFSNSGDDFGIPRILIVGCGGAGGNTINRLKRMGL
                     MGAKTIAINTDRQHLETVFADEKMLIGRKLTRGMGAGGDPEVGRKAAESARTDIEDLL
                     RGADLAFVLAGMGGGTGTGSAPVVARIARQEGALVVAMVTTPFHMERKRIFIAEEGLE
                     NLRNYANTSIVMDNNRLLERAPHLPFQEAFSLVDGITGEIIQGICETLTTPSLINLDY
                     ADVHTIMNTGGASFMLVGEGSMKKSPEKIVRSALNNPLVDVELRGAKACLLHIDGGPD
                     MTLKEAASIASSLTQDLDPRANVIWGAKIKPELRGRVKLMAIITGVKSAQVLGSTEAG
                     IDPLEEMSLPADRCIGLARTRTDAIK"
     gene            3901..>4309
                     /locus_tag="PLV49_03200"
     CDS             3901..>4309
                     /locus_tag="PLV49_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HOG97882.1"
                     /translation="MIRSEVATKMTGQTAARDLAEFIAKNYPGRVVEVGVGHFPHVAQ
                     RLSEMGLEVILTDRVEGLLAGMRVEKDDIFAPRREIYQGAGLIYSIRPPLEMQLAMGE
                     LAAAVGADVIVRPLQDEIAQLAGFGRRLVNYREA"
     assembly_gap    4310..4409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4439..5140
                     /locus_tag="PLV49_03205"
     CDS             4439..5140
                     /locus_tag="PLV49_03205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOG97883.1"
                     /translation="MRLHYIQHNPFEDPANIALWAEDSGHDVFGTKIFAGERLPKDAD
                     FDLLLVLGGEMNIYQEDLYPWLAEEKRFIRRAMDSEKTILGICLGAQLISDLLGGTVH
                     KNRYREIGWHVVKLNRAAFSSFAFKGFPDRFTAFQWHGDTFDLPGGATGLASTTACAN
                     QAFEIGRNVGLQFHLESSWQSIRRLIDNCGDELTPGRFVQEPEDMLSAEKHLKELYKH
                     LGIFMKNIELMVDRD"
     gene            complement(5185..>5505)
                     /locus_tag="PLV49_03210"
     CDS             complement(5185..>5505)
                     /locus_tag="PLV49_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97884.1"
                     /translation="QIDPIDDNIGGFRIYLMASEELDAIKELMVETAEDHGISQEEIN
                     AFEVEHGLDMLGDVFEVIRDRYEILPEIRGDNIIFSLVAFDSQDIDDSKGNDIFWSEE
                     ADTN"
BASE COUNT         1117 a         1428 c         1459 g         1301 t
ORIGIN      
        1 gggatcacct tccagtcggt gccgatcacc agcagatagt ccacatgctt tcccatctcc
       61 actgcgaatt gctcatactt cttgccggcg atgaccacgt atgctgcctt gttcccggag
      121 atggtcttgg ccagagcata gtccatggag ctgttcatat ctttgggcag aggcaccgat
      181 ccatcgccct ctccgtgcct gccgatcacc agaatgtcct ctgcatccct ctctgcgcca
      241 aagcatgcca cctggatctt tcccagctca cgcacccgct cgacgttctc ccggtccaca
      301 attacgcatt cgaatccaga ttcaagggcg gtagttatct ggggtttgat ctcctcccat
      361 gatcccttgg ccatgagcca cttctctttc atatgcgact ctttgacata ccattacaaa
      421 tctcttttgc agctctgcgc ccatatacaa aagattatac gcctgggatg gcaatataga
      481 ggaaaatgga aaaccgtctg atctactctc cagttggcat attgatcatg ctgggcttcg
      541 cgttcttgat tgcagtagta gtgagctttc tcttcctgga cctggccagg acagccttca
      601 ccaagatcgg tttctcctgg agccaggccc tctttgtgct tttggcgtct ctggttggat
      661 ccagcataaa cataccattg accaacctag agtgtagcac tcccatggtc catgaacgac
      721 acattcgggc ctttggcgta tcttatcatg tccctgtggt gaagagctgc aatacccttt
      781 tggccataaa tgtgggcggg gccctgattc cctcattgat ctccatggca ctcatctacc
      841 ggttcccaga gtccatctac tatgcactat ctggcattgt cttggtggcc atcatcacca
      901 accgggtggc aaggccggtt cgagggctgg ggatcgtgac tcctgccctg gtcccacccc
      961 tttgtgccgc cctggcggcg atcctcatgg tctatgtgct gggcgcccct catgatctca
     1021 tcttcctaat cgcttatgcg gcaggaacac tgggcactct gcttggggcg gatattctca
     1081 acctgggaaa gatccgcgac cttggggctc cagtggccag tatcggcgga gctggaacct
     1141 ttgacggtgt attcctgagc ggtctgatcg ctgtgctcct ggtgtgagat ggatctagaa
     1201 atagtggaaa gagatagaat aacaagagat tatataaaaa gatgataaaa ggtggcggaa
     1261 tgaaagcagg cggaataaaa aggagggtaa gatcatgagc ttcaggggct ttctggatga
     1321 tctggaaggg tcaggtttga tgaaccacgt gcgtgaccct gtatcgcccg ttcaggaggt
     1381 cacagagaga tcctggggta gtgggccgat tctatttgac gatgtctccg gccataagtg
     1441 ctgcctgaat atccttggga cgagggacct cctggcccgg gccctgggca tccctgctga
     1501 ggatatggtc cgccacctat cccagatcgg ctgccagggt ccagtgcgag aggtggattg
     1561 gtctagtttc atggagaatg tctcccaacc ggatctcagc aggcttccca tcatgaccta
     1621 ctttccgggg gacggcggcc cttatatcac ctctggagtg gtggtgagcc gctttgagga
     1681 caagatcaat gcctgcgttc accgcctgat ggtcctggga aaagaccggc tggcagcaag
     1741 gctggtcccg ggaaggcata cccatcagct ctatgaggcc gccctggcca agggcaagga
     1801 ggttgcagtg gctgtggcca taggagtgga tccgctggta ttgatggctg cctccacccg
     1861 cgttcctcca gagatggagt tttgttatgc tgccgctctg cgaggctctc atgtggagct
     1921 gattgctctg gataatggag ttcttattcc ccatgcacag atcgttctag agggatacat
     1981 caccactgaa agggcaccgg aggggccgtt cgtggacatc accggcactc gggacctggt
     2041 ccggcaggag ccggtcatcc ggctaaccag gatgatgatg tgcaatgagc ccatctatca
     2101 tggccttctg cctgcaggag gtgagcacaa gatgctgatg ggagtgccct atgagccgct
     2161 tatctaccgg gaggtctcca aggtggcaaa ggtgaaggac gtcatgctta ccgagggcgg
     2221 atgctgttac ttccatgccg tggtgcagat tgagaaggag acggaggagg atgccagaag
     2281 agcgatcgat gccgctatca aggctcatcg ctctttgaag catgtgctgg tggtggactc
     2341 agatatcaac attcacgatc ctcgagacct agagtatgcc ctggctacgc gcatgcgcgg
     2401 tgacgaggat atagttcttt atcccaatgt gcgcggcagc accctcgacc ccaggagcaa
     2461 tgagggaatg accaccaagg tgggtgtgga tgccacnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnccaa atgacccgct ttccgctcaa atctccagat agaggcggat
     2641 gctgtagatc gcggcaatgc ccaggattat cgccagggtt aaggccacta tctggaacaa
     2701 gagttcatcc atgctatcac gctcatcttt attcccctga tatcctcatt ttatggcatc
     2761 agtccttgtc cgggcaaggc caatgcaccg atcggctggc aggctcatct cctctagtgg
     2821 atctatcccc gcctcggttg atcccagcac ctgggctgat ttcaccccgg ttattatggc
     2881 catcagcttc accctgcccc tgagctcagg cttgatcttt gctccccaga tgacattcgc
     2941 cctcggatcc aggtcctggg tcaggctgct ggcgatggag gctgcttcct tcagggtcat
     3001 atccggccca ccgtcaatat gcagcagaca ggcttttgct cctctgagct ccacgtccac
     3061 caggggattg ttcagagcgg agcggacgat cttctccggg ctcttcttca tgctcccctc
     3121 tcccaccagc ataaagctgg ccccgccggt gttcatgatg gtgtgcacat cggcatagtc
     3181 caggttgatt agcgaggggg tggtcaatgt ctcacagatc ccctggatga tttcgcctgt
     3241 gataccatcg actagagaga aggcttcctg gaagggcagg tggggggcgc gctccagcag
     3301 gcgattgtta tccatcacaa tggaggtgtt ggcgtaattg cgcaggttct ccaggccttc
     3361 ctcggcgatg aagatcctct tcctctccat gtgaaatggc gtggtgacca tggccaccac
     3421 cagggcaccc tcttgcctgg caattcttgc gaccaccggc gctgagccgg tgccagtgcc
     3481 tccacccatg cccgcgagga caaatgccag gtctgcgccg cgcaagaggt cctcgatgtc
     3541 cgttcttgcg ctctccgccg ccttccttcc cacctcgggg tctcctcctg ctcccatccc
     3601 ccgggtgagc ttccggccaa tcagcatctt ctcatccgca aagacggtct ccaggtgctg
     3661 gcggtcggtg tttatggcta tggtcttcgc ccccatcagg cccattcttt tgagtctgtt
     3721 gatggtgttt ccgcctgcgc cgccacagcc tacgatcagt attctgggga taccgaagtc
     3781 gtcgcctgaa tttgaaaaat ccatggcctc ccgtatatca agttccatcc ctatcccttc
     3841 attgctttct cataatcaat tagtgctatc tgttaataac aattgcggat agagataaga
     3901 atgatcagaa gtgaagtggc aaccaagatg acgggacaga cggctgccag ggacctggcg
     3961 gagttcatcg ccaaaaacta ccccggcagg gtggtggagg tgggtgtggg ccattttcct
     4021 catgtagctc agaggctgtc ggagatgggc ctggaagtga tccttacgga cagggtggag
     4081 gggcttctgg cgggcatgag ggtggaaaag gacgacatat tcgccccccg gagggagatc
     4141 tatcaggggg cgggcctcat ctactccatt cgccctcctc tggagatgca gcttgccatg
     4201 ggggagctgg cggctgcggt gggtgcggat gtcatcgtcc ggcccttgca ggatgagatc
     4261 gcacagctcg ctggatttgg caggaggctg gtcaattacc gggaggcgan nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng accatcgtaa tgcagggaga gatgagcgat
     4441 gagattgcac tacatccagc acaatccctt tgaggacccg gcaaatatcg ccctctgggc
     4501 ggaagattcc ggtcatgatg tcttcgggac taaaatattt gcgggcgaga ggctccccaa
     4561 agatgctgat tttgatctgc tgctggtttt aggtggggag atgaacatct accaggagga
     4621 tctttatccc tggctggcgg aggagaagag gttcatacgc agggctatgg atagcgagaa
     4681 gactattctt ggaatctgcc ttggcgccca gctgatctca gatctcctgg gaggaacggt
     4741 gcataaaaat cgctacaggg agatcggctg gcatgtggtg aagctgaatc gtgctgcatt
     4801 tagctctttt gctttcaagg gatttcctga tagattcacg gcatttcaat ggcatgggga
     4861 tacatttgac ctgcctggag gagcgactgg gctggcatcg actactgcct gcgccaatca
     4921 ggcctttgag atcgggcgaa atgttggact gcaatttcat ctggagtcat cctggcaaag
     4981 catacgaagg ctgatagaca actgcgggga cgaattgact ccaggaaggt tcgtgcagga
     5041 gccagaggat atgctctctg cagaaaagca tctaaaggag ttatacaaac atttaggaat
     5101 ttttatgaaa aacattgaat tgatggtaga tcgggattga tccggattgc catctttgtg
     5161 ctttttatcc aatcacttgc tgatctaatt tgtatcagcc tcctcgctcc agaagatatc
     5221 gtttcccttg ctgtcgtcta tatcctggct gtcgaaagcc accagggaaa agatgatatt
     5281 gtctcctctg atctccggaa gaatctcgta tctatctctg atcacctcaa agacatctcc
     5341 caacatgtcc agaccgtgct caacctcaaa ggcatttatc tcctcctggg agatgccatg
     5401 atcttctgca gtctccacca tcagctcttt tatggcatcc agctcctctg aggccatcaa
     5461 gtagattctg aaaccgccga tattgtcatc gatcggatcg atctg
//
LOCUS       DAOSGP010000070         5480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13177_length_5480_cov_18.561843, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000070 DAOSGP010000000
VERSION     DAOSGP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13177_length_5480_cov_18.561843"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLV49_03215"
     CDS             complement(<1..191)
                     /locus_tag="PLV49_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97885.1"
                     /translation="MEYDDSGETFNGSMGRNASGYNATMGVQFQGTGEVQTPAPIICE
                     DYNPCTIDYSGPAGCIHDPV"
     gene            complement(611..1081)
                     /locus_tag="PLV49_03220"
     CDS             complement(611..1081)
                     /locus_tag="PLV49_03220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOG97886.1"
                     /translation="MRIRKAEPKDLPQMVQIESLCFPDDTAFPPGMFAYLIRYAVAIV
                     ACEPEDQILGFIIGYNSGNAGAVYTLDVHTGYRRRGIGKELLLSLEKRLAMMGARAIR
                     LEASLEKPEAIELYRKAGYREREIVRNYYGRGCHAVRMWKTLQPLSSLPGIDSA"
     gene            complement(1078..1467)
                     /locus_tag="PLV49_03225"
     CDS             complement(1078..1467)
                     /locus_tag="PLV49_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HOG97887.1"
                     /translation="MSKLVISEEARYEDLADLAIALNEIVRLPVTMRGLKYPGVRVEN
                     GKVVDGNYTGPILEEVIRTGKAIRTIPESGAYKGVPVSVAPIVVEGRTVAAIGIVDVI
                     GTIDIPEVFGAYADVVAQVRGKAPEKK"
     gene            complement(1509..2684)
                     /locus_tag="PLV49_03230"
     CDS             complement(1509..2684)
                     /locus_tag="PLV49_03230"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOG97888.1"
                     /translation="MSEVVLAYSGGLDTSVCIPLLRERYGFDKIVTVLVDVGQPRSDI
                     ERGNLRAKQLADEHYTIDAREEFVEKCLFPLIKANGSYEGYVLGTAIARPLIASKSAE
                     IADKLGIKNLAHGCTGRGNDQLRFEAIFRATDCRVIAPMRELDLAREWEIDYAREHGI
                     DVPVTKDKPWSIDENIWSRSIEGGKLEDPYFEPPEEIYGWTKTPSSDRSAQIVEIGFK
                     EGVPVSLDGKQMNGSELIEKLNQIGGEHGVGRTDMIEDRVMGLKARENYEHPAATILL
                     TAHKDLERLVLSRNELRFKVMVDEFWSELAYMGLVEDPLYADLNAFVDQSQKRVNGSV
                     KIKLFCGSAISVGRQSPDALYSQDMVSFDSQTLSQKDAEGFAKYHGFQARLLKMKRR"
     assembly_gap    2749..2848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2849..3165
                     /locus_tag="PLV49_03235"
     CDS             <2849..3165
                     /locus_tag="PLV49_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOG97889.1"
                     /translation="QIEREKIINIYRKINSTPGGPRLFANTYFEGQMLGCMAGRRWMH
                     VAVDGRVRACPYMQDSYDDIREASLKDAWKRLRSSGQFDGFVSCCPAQGIHAISPSDK
                     KY"
     gene            3230..3859
                     /locus_tag="PLV49_03240"
     CDS             3230..3859
                     /locus_tag="PLV49_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HOG97890.1"
                     /translation="MSSFRENLGGWIRKIFKKKRARIGLYGPPNAGKTTLANRILKDW
                     STGDAIGTVSPIPHETRRAMRREGLIINANGSSIELDIVDTPGMATKIDFKEFMAFGL
                     SEAESKKRAKEATEGVIEAIKWLDDLDGVLLVMDSTEDPYTQVNVTVIGNMEARKLPL
                     LIVANKMDLPNASPSRISAAFPQHSIVPISALEGSNIDDLYQAIATHFG"
     gene            3880..4224
                     /locus_tag="PLV49_03245"
     CDS             3880..4224
                     /locus_tag="PLV49_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HOG97891.1"
                     /translation="MDLISEEKLENMTSMEKIRLILDKVKTGKIVVLESGLTPDEEVR
                     LIEMTMTEIRVDEFSGIEIESYPSKKGGSFLDRIFGKGLKGRMTVIGPANQIHTIEKD
                     EFQIRTKVSVGD"
     gene            4225..4641
                     /locus_tag="PLV49_03250"
     CDS             4225..4641
                     /locus_tag="PLV49_03250"
                     /inference="COORDINATES: protein motif:HMM:NF021372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing protein"
                     /protein_id="HOG97892.1"
                     /translation="MPHICTRCNSVFESGEDILKGCPSCGWKKFMFVRKMPQGGDISS
                     VSQDRIISRAMGVRMPPKKREMPLAVDRDEPSIREFGNGVKPEGEKLESIKITAPGTY
                     ELNLPSLFEREELIMAVKEGTYFIDLTSVFRKSKKD"
     gene            complement(4658..4819)
                     /locus_tag="PLV49_03255"
     CDS             complement(4658..4819)
                     /locus_tag="PLV49_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97893.1"
                     /translation="MIEMTVELKVDGKEIPLSEFPQEIIGNTAAAMAQSLRGVDENWK
                     VIEIRISQD"
     gene            complement(4839..5249)
                     /locus_tag="PLV49_03260"
     CDS             complement(4839..5249)
                     /locus_tag="PLV49_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97894.1"
                     /translation="MKSILTIFAASAFLLGIMAAASAADVIELSTLGKKSQVDAISLP
                     QLSPTMPTIAPAALGSNISSNVMDLSTLGKKAVSTVPATALMGATPITITPMFAVRNA
                     NTSNVAGSTVFTLPQAVSSGNSVYTPPIAIFGGA"
BASE COUNT         1340 a         1366 c         1351 g         1323 t
ORIGIN      
        1 acaggatcat gtatgcagcc tgcagggccg ctgtaatcga tggtacaggg attgtagtcc
       61 tcgcagatta tgggagcagg agtctgaacc tcgccggtcc cctggaactg cactcccatg
      121 gtggcattgt agccacttgc attcctaccc atgctgccat tgaaagtctc gccggaatca
      181 tcatattcca tgaatgagtc ctcaaagcag ctctgaggag tatgctcgca tttcgttcca
      241 ttgcagaagt ccttggtaca gggatttccg tcgtcacagt ctccgcactg ggccgagacg
      301 ctactgggga tcagcagccc acagagaatg aatgttatta gatattggca aagcttgcct
      361 ttagtatatc cttttatcac acttcacacc ccgaacttct tttgctctca atcccattta
      421 tatctatttt tcgctctctc cgatgtctat acttttcaag aacctatata aacgattcaa
      481 tagcagatct gaatcctgat acccagttta tcggtaaacc aggccagcaa gcactaccat
      541 taaactaata gagtcttaga tttagggcta agcccaccag aggaagaatt agcggcattt
      601 gagataaaag tcatgcacta tcaattccag gcagcgagct taaaggctgc agggtcttcc
      661 acatccgcac agcatggcat ccccggccgt agtagttccg gacaatctcc ctttccctgt
      721 accctgcctt gcggtagagc tcgatagcct ctggcttctc cagggaggcc tccaggcgaa
      781 tagccctggc acccatcata gcgagcctct tctccaggga cagcaatagc tctttgccaa
      841 ttcccctcct ccggtagccg gtatggacat ccagagtgta gaccgctcct gcattcccgc
      901 tgttatatcc gattatgaat cccaatatct gatcctcagg ctcacaggcg actatcgcca
      961 ctgcataacg gatcaggtag gcaaacatcc ccggcggaaa ggctgtatca tccggaaagc
     1021 agagcgattc gatctgcacc atctgtggca gatcctttgg ctctgctttt cgaatcctca
     1081 ttttttctca ggagccttcc ctctaacctg agctactaca tcagcatagg ccccgaagac
     1141 ttctgggata tcgatcgttc caatcacatc cactatgccg atggcggcaa cagtccgtcc
     1201 ctccaccact atgggtgcaa ctgatactgg aacgccctta taggcaccgc tctcgggaat
     1261 ggttcgaatg gccttcccgg ttcttatgac ctcttccagg atcgggccgg tatagttccc
     1321 atccacgacc ttgccgttct ccacccgaac gcctggatat ttcaatcctc tcatggttac
     1381 aggaagccga acgatctcat tgagggctat tgcgagatct gccagatcct catatctggc
     1441 ctcttcagat atcaccagtt tggacatatc ggtattgtat ggaggcgata tcaaataaat
     1501 gctctccttt accttctctt catcttcaat aacctggcct ggaagccatg atacttggca
     1561 aagccctctg catccttctg gctcagagtc tgggaatcga aggagaccat atcctgggaa
     1621 taaagggcgt ccggtgactg gcggcctact gaaatggcgc tgccgcagaa gagctttatc
     1681 ttaaccgagc cgttcaccct cttttgggac tggtcaacga aggcattcag gtcggcatag
     1741 agggggtctt ccaccaggcc catataagcc agctcagacc agaattcgtc caccatgacc
     1801 ttgaagcgga gctcattgcg agataatacc aggcgctcca gatccttatg ggccgtcagg
     1861 aggatggtgg cagcaggatg ctcataattc tctcgcgcct tcagccccat aacccggtcc
     1921 tcgatcatgt ccgtcctgcc cacgccgtgc tcgccaccga tctgattgag cttctcgatc
     1981 agctcggagc cgttcatctg ctttccatcc aatgagacgg gcacgccctc tttgaatccg
     2041 atctcaacta tctgggcgga ccggtcagag ctaggggtct tcgtccagcc atagatctcc
     2101 tccggcggct cgaagtaggg atcctcgagc tttccccctt caatcgaccg agaccagatg
     2161 ttctcatcga tagaccaggg cttatctttg gtcacaggca catcgatgcc gtgctcgcgc
     2221 gcatagtcta tctcccactc gcgagccagg tccagctctc tcatgggagc tatcacccga
     2281 caatccgtgg ccctgaatat ggcttcgaag cggagctgat cgttgccgcg gccggtgcat
     2341 ccgtgggcca ggttctttat ccccagctta tcagcaatct ctgccgattt gctggcgatg
     2401 aggggacgag caatggccgt tcctagaacg tatccctcat atgagccgtt ggccttgatc
     2461 agggggaaga ggcatttctc cacgaactcc tccctggcgt ctatggtata atgctcatcc
     2521 gccagctgct ttgccctcag gttccctcgt tctatatccg accttggttg gccgacgtcc
     2581 accagaacgg taactatttt atcaaatccg tagcgctccc ggaggagagg aatgcaaacc
     2641 gatgtatcca agccaccgga ataggccagt actacttcag acactttgtc aacaccattt
     2701 tggtacagga ggatggatcg atttaatctt atctcttaaa attccagcnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cagatcgaga gggagaagat cataaacatc
     2881 taccgcaaga tcaattccac accaggtggt ccccgtcttt ttgccaatac ctatttcgaa
     2941 ggccagatgc tggggtgcat ggcgggaagg agatggatgc atgtggcagt tgacggcagg
     3001 gtcagggcct gtccatacat gcaggatagc tatgacgata tacgcgaggc atcgcttaag
     3061 gacgcctgga agaggttgcg cagcagcggc cagttcgatg gctttgtatc ctgctgtccg
     3121 gcccagggca tccatgctat ctctccatca gacaaaaaat attgataaga catggaaaat
     3181 tattagtaca gatatgtcct agtctgatct gaggtagtac atgggtttca tgagcagctt
     3241 cagggagaac cttggaggct ggataagaaa gatcttcaag aagaagaggg ccaggatcgg
     3301 attgtatggc ccgcctaatg ccggaaagac cactctggcc aatcgaatac taaaggattg
     3361 gagcacagga gatgctattg gaacggtctc tccgattcct cacgagaccc gcagggcgat
     3421 gaggagagaa gggcttatca tcaatgccaa tggatcgagc atcgagctgg atatagttga
     3481 tacgccgggg atggcgacaa agatcgattt taaggagttt atggcctttg ggctctcaga
     3541 ggctgaatcc aagaaaagag ccaaggaggc cacagaaggg gttatagagg ctatcaaatg
     3601 gttagacgac ctggatggtg tattgttggt tatggactcg accgaagatc cctacaccca
     3661 ggtcaatgtg acagtgatag gcaatatgga agcgagaaag ttgcctcttc ttattgtggc
     3721 caataagatg gacctgccta atgcttcccc ctccagaatc agcgctgcct ttccccagca
     3781 ttccatcgtc ccaatctctg ctttagaggg atcgaatata gatgaccttt atcaggcaat
     3841 cgcgactcac tttgggtgat cttgatgaga gaagtgcata tggacctaat ctccgaggag
     3901 aagctggaga atatgacatc tatggagaag atcaggctca tcctggacaa ggtcaaaacg
     3961 ggaaagatcg tggtccttga gagcggcctg actccagatg aggaggtgcg actcatcgag
     4021 atgaccatga ctgagatcag ggtggatgag ttttcaggaa tagagataga gagctaccca
     4081 tccaagaagg gaggatcgtt tttagatcgg atctttggaa agggcctcaa ggggaggatg
     4141 acggttatcg gcccggcaaa tcagatccat accattgaga aggatgagtt tcagatcaga
     4201 acaaaggtct ctgtggggga ttgaatgcct cacatctgca cccgatgcaa tagtgtattc
     4261 gaatcgggtg aggatatcct caaaggctgc ccatcctgcg gctggaagaa gttcatgttc
     4321 gtgcgcaaga tgccgcaggg cggggacata tcttccgtct ctcaggatcg gataatatcc
     4381 agggcgatgg gggtaaggat gcctcccaaa aaaagagaga tgccactggc cgtggatagg
     4441 gatgagccat cgataaggga gtttggcaac ggggtaaaac cggagggtga aaagctagag
     4501 agcataaaga taacagcgcc aggaacctat gagttgaatc tgccgtccct attcgagagg
     4561 gaggagctta tcatggcagt caaggaagga acttatttta tcgatcttac atctgtattc
     4621 agaaaaagta aaaaggattg agagatgatc ttcgcagtca atcctgcgat atgcggatct
     4681 cgattacctt ccagttctca tctactcctc gcagagactg ggccatggca gctgctgtat
     4741 tgcctatgat ctcttgaggg aactcgctca atggaatctc tttgccgtcc accttcagct
     4801 ctacagtcat ctcaatcata tcaataaaat tatatctttc aagctccacc gaatatggct
     4861 attggagggg tgtagacgga attaccagat gagactgcct gggggagagt gaacacggtg
     4921 cttcctgcta cattgcttgt attggcattc ctaacagcga acatgggcgt gatggtgata
     4981 ggcgttgcgc ccattaaggc agttgcgggc accgtggaca ccgccttctt cccgagggtg
     5041 ctgagatcca ttacattgct tgatatattg cttcccagag cagctggcgc gattgtgggc
     5101 atggtgggag agagctgggg cagagagata gcatctacct gggatttttt gcctaatgtg
     5161 ctcaattcaa tgacatccgc agcgctggcc gcagccataa tgcccaatag gaaagcgctg
     5221 gcagcgaaga ttgtgagaat tgacttcatt tatgaacctc ctttcagaaa gggtgtatac
     5281 cctatgattt ctcgtacatg ggcttttacc atttattctt tttgcctatt caataagagg
     5341 ctgcactcct ctaatccaat atcagtatgc tgctgttaca caacattaag gtaactcata
     5401 tgataataat tatataaaaa aaaaaataaa gactgctgga tcttatctag cagtctatgc
     5461 cgtcgcaagg cggatgatcc
//
LOCUS       DAOSGP010000071         5477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13185_length_5477_cov_17.615824, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000071 DAOSGP010000000
VERSION     DAOSGP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13185_length_5477_cov_17.615824"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLV49_03265"
     CDS             complement(<1..338)
                     /locus_tag="PLV49_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOG97895.1"
                     /translation="MYRPVSESYSTINPCNMCMPMGSVLAFRGVEGCMPLFHGSQGCA
                     TYMRLYLAHHFREPVDIASSALSEKGTVYGGSANLKMGLKNLIIGYSPKAIGVATTCL
                     AETIGDDIVQ"
     gene            complement(319..1746)
                     /gene="nifE"
                     /locus_tag="PLV49_03270"
     CDS             complement(319..1746)
                     /gene="nifE"
                     /locus_tag="PLV49_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HOG97896.1"
                     /translation="MSVIKTFEGREGHISQKAGYDPVSLSCSNESLAGAVSQRACVYS
                     GARVVLNPLTDALHLVHGPIGCASYTWDIRGSSSSGPDLYKNSFSTDMSELDVVYGGE
                     KKLARAIRDLAERFHPPAIFVYSTCIVGIIGDDLKAVCRSATAELGLPVIPVRSEGFR
                     GNKNEGYKAACNALFELVGTKEYRPAIPYAINLLGEYNVAGDLWAIKPLFEEMGIEVV
                     ATFTGDGRVAEIQRSHTARLNLVQCSGSMTYLAKKMEERYGIPYQHISFFGLGDLASA
                     LRCAARFFADQSMLAKAEEIIERETARVMPKIVYYRSRVQGKKAAIYMGGAAKAVSLV
                     RAFEELGMEVVIIGTQTGDRDDYRKISCMVRDGTVIVDDANPLELRELLAVQKADLLV
                     AGVKERFLAYKLGIAFCDFNHDRTTSFEGYDGLVNFAQELDASLNSPVWRLPLRRSSP
                     IVGSQGSEIEKTIEKAAEGGVQAGV"
     gene            complement(1759..>2355)
                     /locus_tag="PLV49_03275"
     CDS             complement(1759..>2355)
                     /locus_tag="PLV49_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOG97897.1"
                     /translation="GKFQIPAVIGPMPIGIRNSDAFVMNVSSLMDVPIPQEIEEERGR
                     LVDMMTDAHPHFHGKSLAIFGDPDTVVGLVSLAVEMGMNPAYALTGTPDKKFEEEVHS
                     VSPDCEAMIGDTFLLHQKIKNNPVDMLVSNTYGKLIARAEDIPLVRVGFPITDRANLH
                     YFPIIGYAGAARLVEMIGNAFLERRDRDSDDTHFEMVL"
     assembly_gap    2356..2455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2456..2766)
                     /locus_tag="PLV49_03280"
     CDS             complement(<2456..2766)
                     /locus_tag="PLV49_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOG97898.1"
                     /translation="MLDYTPKEMAQRETLVINPAKICQPIGAILAYKGIHNCMPITHG
                     SQGCSSYLRMVLARHYREPTLAATSSFTEDSVVFGGRNNLKEAVDNVVNIYHPDVLAI
                     NT"
     gene            complement(2759..4369)
                     /gene="nifD"
                     /locus_tag="PLV49_03285"
     CDS             complement(2759..4369)
                     /gene="nifD"
                     /locus_tag="PLV49_03285"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HOG97899.1"
                     /translation="MSGVEREPVTIARSRQTVEGMVKGLPQKTRDIRKKHVVVKDSCE
                     IEQRIEANDRSVPGILTNRGCAYAGSKGVVFGPIKDILHITHGPIGCAYFTWGTRRNL
                     MSQDEGRDCYSGYCLSTDVKETDIVFGAEKKLAAAIREAYAIFKPECISVFCTCPIGL
                     IGDDIESVCRKAEEDLRIKVIPVRCEGYRGVSQSAGHHIASNALMEHLVGTEELENPG
                     PFDVNVFGEYNIGGDYWVVKDLLEKVGYRIVSPFTGDASFHDIAKAHRAKLNILLCHR
                     SINYTNRMMEEKYGIPWLKVNYLGVEDTIKTLRDMAAFFDDPGLTRRTEAVIAEELAK
                     IQPTIDMYRKKLAGKRVMILVGGSRAHHFRNMFETLGMEVVVAGYEFAHRDDYEGRKI
                     LTSIVHTGRSKVLEDIHYERDPNVVSPYDNEALQKKKEKIPRLMDYEGLLPHMKDGQI
                     MIDDYSHQECERLVRELGVDLFCSGIKDKYVFQKMHIPSRQMHSYDYSGPYTGFSGFV
                     QFAKDIDMSVNSPTWKFITPPWRQKSNA"
     gene            complement(4366..4740)
                     /locus_tag="PLV49_03290"
     CDS             complement(4366..4740)
                     /locus_tag="PLV49_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOG97900.1"
                     /translation="MKEVMAIIQLNKMEATKDALEVIGISSFTAYKASGRGKQKGLQI
                     PHPTPLDERDERDERDEKRMKYIPKRMISVMVEDEFVPAVVAVITKVNRTGNYGDGRI
                     FVSPMEESVRIRTGERGSVAIS"
     gene            complement(4744..5061)
                     /locus_tag="PLV49_03295"
     CDS             complement(4744..5061)
                     /locus_tag="PLV49_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOG97901.1"
                     /translation="MKMIRAVIRPEAVDKVADSLEAGGFTALTRIEVFGRGKQKGIKV
                     GNVVYDNLPKTMIMLVVDDDSVEKSVKIIEDSARTGNIGDGKIFVSSVDEAYTIRTGQ
                     RGL"
     gene            complement(5058..>5477)
                     /gene="nifH"
                     /locus_tag="PLV49_03300"
     CDS             complement(5058..>5477)
                     /gene="nifH"
                     /locus_tag="PLV49_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718497.1"
                     /note="nitrogenase iron protein; nitrogenase component 2;
                     with component 1, an molybdenum-iron protein, catalyzes
                     the fixation of nitrogen to ammonia; nitrogen reductase
                     provides electrons to the nitrogenase complex; in R. etli
                     there are three essentially identical copies of nifH which
                     are actively expressed during symbiosis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase reductase"
                     /protein_id="HOG97902.1"
                     /translation="MPIREGKAQEIYIVASGEMMALYAANNICKGIQKFAESGGTRLG
                     GIICNSRNVDRERELVSAFAEKIGSQMIQFVPRDNIVHQSEIHKQTVIQYAPDSDQAK
                     AYLDLARAVDLNQMFVIPKPMEYEELEQVMMEWGVVE"
BASE COUNT         1049 a         1561 c         1423 g         1344 t
ORIGIN      
        1 atctggacga tgtcgtctcc aatggtctcg gcaagacagg tcgtggctac gccgatggcc
       61 ttggggctgt aaccgatgat gaggtttttc agacccatct tcaggttggc agagccacca
      121 taaaccgtcc ccttctcgct cagggccgag gatgcaatgt ccactggctc tcggaagtgg
      181 tgggcgaggt acaatcgcat gtaggtagca cagccctgag agccgtgaaa cagaggcatg
      241 cacccctcga ctccccggaa agccaggacc gatcccatgg gcatgcacat attgcaggga
      301 tttattgtgg aatagctctc agacaccggc ctgtacacct ccttctgctg ctttctcgat
      361 agttttctcg atttctgaac cttgggaacc cacaatagga ctcgatctgc gcaggggcag
      421 cctccagaca gggctgttca ggctagcatc cagctcttga gcaaagttca ccaggccgtc
      481 atagccctca aagctggtgg ttcggtcgtg gttgaagtcg caaaaagcga ttccaagttt
      541 ataagcaaga aacctctctt tgacccctgc caccagcaga tcggcctttt ggactgccag
      601 cagctccctc agctcaagag gattggcatc gtccacaatt accgtcccgt ccctgaccat
      661 gcagcttatc ttccggtagt cgtcccggtc accggtctgc gttcctatga tcaccacctc
      721 cattcccagc tcctcgaaag ctctgaccag ggagactgct ttggcagcgc cacccatgta
      781 gatggcggcc ttctttccct ggacccggga acggtagtag acgatctttg gcatgacccg
      841 agccgtctcc cgttcgatta tctcctctgc ctttgccagc atgctctggt ctgcaaagaa
      901 tcgagcagcg cagcgcagag ctgatgccaa atccccaagg ccgaagaagc tgatgtgctg
      961 gtagggaatg ccatacctct cttccatctt ctttgcgagg taggtcatgg agccgctgca
     1021 ctggaccaga ttcagccggg cggtatgaga tctctggatc tctgctaccc tgccgtctcc
     1081 agtaaatgtg gcgaccacct cgatccccat ctcctcaaat agaggcttaa tggcccagag
     1141 gtctccggca acgttgtact ccccgaggag gtttatggca taggggatgg caggcctgta
     1201 ttccttcgtg ccgaccagct cgaatagagc attgcaggca gccttgtagc cctcattctt
     1261 gtttccgcga aagccttccg accggacagg aatgacgggc aggccaagct ctgcggtagc
     1321 gctacggcag actgccttca ggtcatcacc aataatcccc acgatgcagg tggaatagac
     1381 gaatatggcc gggggatgaa acctctccgc cagatcacga atggctcttg ccagcttctt
     1441 ctcgccgcca taaacgacat ccagctccga catgtccgtg gaaaagctgt tcttatagag
     1501 gtccggaccg gaggaggaac tccctctgat atcccaggta tagctggcac agccgatggg
     1561 gccgtgcacc agatgaaggg catcggtgag gggattgagc actacccggg ctccggaata
     1621 aacgcatgcc ctctggctga cggcaccagc gaggctctcg ttgctgcagg agagggacac
     1681 aggatcatag cccgctttct gggatatgtg accctctctg ccttcaaagg tcttgatcac
     1741 gctcatggta gttgtttcct acaggaccat ctcgaaatga gtatcgtctg agtctctgtc
     1801 acgcctctcc aggaaggcgt tgcctatcat ctccaccagg cgggcagctc cggcgtagcc
     1861 gatgatgggg aagtaatgca gattggcccg atcggttatg gggaacccca cccgcaccag
     1921 cggtatatcc tctgccctgg ctatcagctt gccataggta ttactgacga gcatatccac
     1981 cggattgttc ttgatcttct ggtgcaggag gaatgtgtct cctatcattg cttcacagtc
     2041 tggcgagaca ctatgaacct cttcttcgaa cttcttgtcc ggagtgcccg tgagcgcata
     2101 tgcggggttc atgcccatct ccactgccag gctgaccagt cctacgacgg tatccggatc
     2161 tccaaagatg gccaagctct tgccatggaa gtgtggatgg gcatcggtca tcatgtccac
     2221 caacctgcct ctttcttcct ctatctcctg gggaatgggc acatccatga ggctggagac
     2281 gttcatcaca aatgcatcgg aattacgaat gccaatgggc atggggccga tgacggcggg
     2341 aatctggaac ttgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtatt
     2461 gatggcgaga acatcgggat gatagatgtt caccacgtta tcaaccgcct ctttgaggtt
     2521 gtttctgcct ccgaatacca ccgagtcctc tgtgaatgag gaggtagcag cgagcgtggg
     2581 ctctcgatag tggcgggcca ataccatgcg caggtatgag ctgcacccct gcgagccgtg
     2641 ggtgatgggc atgcagttgt ggatgccttt gtaggccagg atggcgccga tgggctggca
     2701 gatcttggcc gggtttatca ccagggtctc tctttgcgcc atctcttttg gggtatagtc
     2761 aagcattact cttctgcctc cagggtgggg ttatgaattt ccaggtgggg ctattcacag
     2821 acatgtcgat gtccttggcg aactgcacaa agcccgaaaa gccggtgtaa gggccgctgt
     2881 agtcataaga gtgcatctgg cgggagggaa tgtgcatctt ctggaagacg tacttgtctt
     2941 ttatccccga gcagaagagg tccaccccca attcccttac cagccgctcg cactcctgat
     3001 ggctgtagtc atcgatcatt atctggccat ccttcatgtg ggggagcagg ccctcgtaat
     3061 ccatgagcct cggaatcttc tccttcttct tctggagcgc ttcgttgtca tacggcgaga
     3121 cgacgttcgg gtcgcgctcg tagtggatgt cctcaaggac tttggagcgt ccggtgtgga
     3181 ctatgctggt gaggatcttt ctgccttcgt agtcgtcccg gtgggcgaac tcgtatccgg
     3241 ccacgactac ctccattccc agagtctcga acatgttcct gaagtggtgc gctcttgagc
     3301 caccgaccag gatcatgacc ctctttccgg ccagcttctt cctgtacata tcgatagttg
     3361 gctggatctt ggccaattcc tcggcaatca cggcttcagt tcttctggta aggccgggat
     3421 cgtcgaagaa cgctgccata tcccgcagtg tcttgatggt atcctccact cccaggtagt
     3481 tgaccttcag ccaggggatg ccgtacttct cttccatcat gcggttggtg tagttgatgg
     3541 agcggtggca gagcaggatg ttcagctttg ccctgtgagc tttagcgata tcgtggaacg
     3601 aggcatctcc agtgaagggg ctcactatgc gatagcccac cttctctagc aggtccttga
     3661 ccacccagta gtctccaccg atgttgtact ctccgaagac gttgacgtcg aagggtccgg
     3721 gattctccag ctcctccgtg cccaccagat gctccatcag agcgttggag gcgatgtggt
     3781 gcccagcgga ttgagaaacg ccccggtagc cttcgcatct caccggtatc accttgatcc
     3841 gcaggtcctc ctctgctttt cggcagaccg attctatatc gtcgccaatg aggccgatgg
     3901 ggcaggtgca aaagacagag atgcactccg gcttgaagat ggcataggcc tccctgatag
     3961 ctgcagccag cttcttttcc gctccaaaga cgatgtccgt ctccttgacg tctgtactca
     4021 gacagtagcc agagtagcag tctctgccct catcctggct catcaaattg cgacgtgttc
     4081 cccaggtgaa gtaggcgcag ccgatggggc cgtgggtgat gtgcaggatg tctttgatcg
     4141 gcccgaaaac cacccccttg gagccggcat aagcgcaacc gcggttggtc aggatgcccg
     4201 gaacgctgcg gtcgttggct tcgatcctct gttcgatctc gcaggagtcc ttgactacaa
     4261 catgcttttt tctgatgtcc cgcgtttttt ggggaagtcc tttcaccatt ccttcgaccg
     4321 tctgccggct ccgggcaatg gttactggct ctctttcgac tccgctcatg atatggccac
     4381 gctccctctc tctccggttc ttattctgac tgattcttcc atcgggctga cgaagattcg
     4441 gccatcgcca tagtttccag ttcggttgac tttggtgatc actgccacta ccgcgggaac
     4501 gaattcgtct tccaccataa ccgagatcat cctcttgggg atgtatttca tcctcttttc
     4561 atccctctca tctctctcat ccctctcatc cagcggcgtt ggatgaggta tctgcagacc
     4621 tttctgcttt ccccgtccag aagccttgta ggcggtaaag ctgctaattc cgatgacctc
     4681 cagtgcgtct ttggtcgcct ccatcttgtt cagctggatg atggccatta cttctttcat
     4741 tttctacagc cctctctggc cggttcggat ggtgtaggct tcgtctacag agctgacgaa
     4801 gatcttgccg tctccaatat tcccggtcct ggcactgtcc tcgatgatct tgaccgactt
     4861 ctcaacgcta tcgtcatcga caaccagcat gatcatggtc ttgggaaggt tgtcatacac
     4921 aacgttgcca accttgatgc ccttctgctt gcctctgccg aagacctcga ttcgggttaa
     4981 ggcagtgaag ccgcctgcct ccagggagtc agcgacctta tccacagcct ccggtcttat
     5041 gactgcccgg atcatcttca ttctactaca ccccattcca tcatgacctg ttccagctct
     5101 tcgtactcca tcggcttcgg tataacgaac atctggttca gatcgaccgc cctggcgagg
     5161 tccaggtagg ccttggcctg atctgagtcc ggagcgtatt gaatcactgt ctgcttgtgg
     5221 atctctgact ggtggacgat gttgtccctg ggcacgaact ggatcatctg gctgccgatc
     5281 ttctcggcaa aagcgcttac cagctccctc tcccggtcga cgtttctgga gttgcagatg
     5341 atgccgccca gacgcgttcc gccggactcg gcgaacttct ggatgccctt gcagatgttg
     5401 ttggcggcat aaagagccat catctctccg gaggccacga tgtagatctc ctgggccttt
     5461 ccttcccgga tgggcat
//
LOCUS       DAOSGP010000072         5441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13293_length_5441_cov_20.874861, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000072 DAOSGP010000000
VERSION     DAOSGP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13293_length_5441_cov_20.874861"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PLV49_03305"
     CDS             <1..933
                     /locus_tag="PLV49_03305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG97903.1"
                     /translation="ITGARIHFHDWVQDKVKGYRLSTLISRSAFDQFLLQKAIETGAS
                     VSFSRVLNYSSKEDHVSVQTGDQIYRSRFLVIASGCQDPLRNQISGKASRDDIGVCLV
                     TEIEADDRDIEARLGSLLDIHFGIASGGYGWIFPHRGYYSVGIGGLASQIRYPRMIMK
                     GFLEDNGFERDQTIIHGHTIPQGGKKKRIALGRVLLTGDAASLVDSFTGEGIYYALRS
                     GEMAARAIGEEKERDVARLYEKRCNKELGEELRYALILSQAMHSHPKAFSWMLERHNE
                     VLDRYIEIAAAKRSYKDFVRWLVPRLPMGILSSN"
     gene            complement(948..1277)
                     /locus_tag="PLV49_03310"
     CDS             complement(948..1277)
                     /locus_tag="PLV49_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOG97904.1"
                     /translation="MKKIEAIVRPERLERVKEALEEIGVVALTTTEIQGRGEQKGIKL
                     QYRGGTMEVDLLTKLKLELYVADESVDPVLKAICDAARTGKFGDGRIFVSPVERSVKV
                     RTGEVFT"
     gene            complement(1293..>2477)
                     /locus_tag="PLV49_03315"
     CDS             complement(1293..>2477)
                     /locus_tag="PLV49_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOG97905.1"
                     /translation="AWVLVSTAMVMLMTPGVGLFYGGLVRKKTVVSMIGLSFLAFALA
                     SVQWVFLGYSLSFGQDIGGAIGALDYLALNGIGLGEAPLAENLPHLLYVAFQMVFAAV
                     TLAILTSAVAERVRLGSFIVLALLWTTLVYDPLAHWVWAGGWLAQMGALDFAGGTVVH
                     ISSGFSALAIAMVIGKRVGFGSYVMEPHNIPMAMLGAGLLWFGWFGFNAGSALGANGL
                     AASALVVTNTSAAAGALTWLLSSWLKGKPSALGMVSGGIAGLVAITPAAGFVDVLGAL
                     VIGAVAGVLCYMSLLFRVSRGLDESCDAWAVHGMGGLWGAVATGIFATSAVNSYTGLL
                     QGNFNQFAVQIMAAGISVVYAFVVTYALAKLVDSFMGLRVTEDEEYVGLDISQHGEKA
                     YA"
     assembly_gap    2478..2577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2578..3222
                     /locus_tag="PLV49_03320"
     CDS             <2578..3222
                     /locus_tag="PLV49_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97906.1"
                     /translation="AKKHLGEGSDFRIADVMETMEGIAPFLDKEVIIGLKCKNCDSYA
                     RGSFPLSNDFWAVDENEHCHEKKTSLKNLNALKLAGCHQYSCQEREELMKLGQDILDE
                     DLRPGFSDRKLYDRSKFASYSVDFRMLSNPGNICIEFGHLTDGGFTCSDIRLYIIKRL
                     DPGFGEEASRKISEALKNGPVMALEANLQIRRQLDIDYVLEWIAKKRKMDIVYL"
     gene            3273..5090
                     /locus_tag="PLV49_03325"
     CDS             3273..5090
                     /locus_tag="PLV49_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HOG97907.1"
                     /translation="MAQPLVPDTSVVIDGRVSARIKSGELQGRRIVVPEAVVAELEAQ
                     ANHGREIGLKGLEELRKLSELAKAGRIELEYVGIRPNLDQIKLAGGGEIDAMIRDVAL
                     ELGASFLTSDQVQSRVAEAMGLDVEYVRPQREAIKPLSLERYFTEDTLSIHLKESAIP
                     MAKRGHVGDFSLVRLGERPSSERELRDLSREIYERAKADPDGYVEMEKIGAAIIQIGP
                     MRIAIAKPPFSDGLEITAVRPVAKVSFDSYRHSSMLKTRLKEGQRGILIAGPPGSGKS
                     TFAAGLAEYLLDCNYVVKTLESPRDLQVPPEITQYSPLEGSMEASADILLLVRPDYTI
                     YDEVRKTRDFQVFADMRLAGVGMIGVVHANKPIDALQRLLGRVELGMIPQVVDTVVFI
                     ERGEVTKVLDVEFVVKVPSGMVEADLARPVIVVKDFESGQAEYEVYSYGEEIVVMPVT
                     REERKPSWNLASREIEKEISHHVRGPFKVEMLTDSSAVIHVPQEDAARVIGKAGKNID
                     QIERSLGMHIDVRARDEPSVPEPRMEETAKHIILWLDGMAGESVDVSVGDEPAFTATV
                     GRRGDIRIAKSSEMAKRIIKKMKKGESIEVHRSGSEWDG"
     gene            5078..>5441
                     /locus_tag="PLV49_03330"
     CDS             5078..>5441
                     /locus_tag="PLV49_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97908.1"
                     /translation="MGWMRYPVSILILLFLAAVLTLSASAQDPEPWQDQRGNATPVLM
                     GKNVEEAEVGQEATGLKSRLLSPMVRWELDLKDSLDRSVEMEMYQSNDALFGKGRIMA
                     DGIEQNVSASGTISGNKLD"
BASE COUNT         1291 a         1410 c         1594 g         1046 t
ORIGIN      
        1 ataaccggag ctcggatcca cttccatgat tgggttcaag ataaggtcaa gggctacaga
       61 ctcagcactc tgatcagccg gagcgccttt gaccagttcc tcctccagaa ggcaatcgag
      121 accggggcca gtgtctcatt ctctcgtgtt ctgaactaca gcagcaaaga ggatcatgta
      181 tccgttcaga caggtgatca gatctataga tcaagatttt tggtcattgc ctccggctgc
      241 caggatcctt taagaaacca gatatcggga aaggcctcaa gagatgatat cggggtctgt
      301 ctggtaacag agatcgaagc ggatgatagg gatattgagg ccagactggg cagccttctg
      361 gatattcatt tcgggatagc aagcggaggc tatggctgga tcttccctca tcgtgggtac
      421 tactccgtgg gcataggagg cctggcatcc cagatccgct atccccgcat gataatgaaa
      481 ggatttcttg aggacaacgg ctttgagcgg gatcagacaa taattcatgg ccacaccata
      541 ccgcaaggag gtaagaagaa gaggatcgcc ctgggcagag tgctcttgac gggggacgca
      601 gccagcctgg tggattcctt cactggtgag ggcatctatt atgctttgcg ttcgggagag
      661 atggcggcaa gggcgatagg agaggagaaa gagagagatg ttgcccggct ctatgagaag
      721 agatgcaaca aagagctggg cgaggagctc agatatgcct tgatcctgtc ccaggccatg
      781 cacagtcatc ccaaagcctt ctcctggatg ctggaacggc ataatgaggt attagacagg
      841 tatattgaga tcgctgctgc caaaaggtct tataaggatt ttgtgcgctg gctggtgccc
      901 aggctgccca tggggatatt gtcctccaat tgaaagtcaa aaaaaattca ggtgaagacc
      961 tctcccgtcc tcaccttcac cgatctttcc accgggctca cgaagatcct gccgtccccg
     1021 aacttgccgg ttcgagcagc atcgcagatg gccttcagca ctggatcgac agattcatca
     1081 gctacataaa gctccagctt gagcttggtt aagaggtcga cctccattgt gcctccccgg
     1141 tactggagct tgattccttt ctgctcacct ctgccctgga tctcagtggt ggtcagggcg
     1201 accactccta tctcctccag agcctctttg accctctcca gtctctccgg ccgtactatt
     1261 gcctcgatct ttttcattat cattcaccgc cttcaggcat aggccttctc cccatgctgg
     1321 gatatatcca ggcccacata ctcttcgtcc tcagtcacgc gcaagcccat gaacgagtcc
     1381 accagcttgg ccagggcata tgtcaccaca aaggcataga ccaccgatat gcctgcggcc
     1441 atgatctgca cagcgaactg attgaagttg ccctgcaata ggccagtgta gctgttgact
     1501 gcggaggtgg cgaatatgcc cgtagccacc gctccccaca ggccgcccat gccgtggacc
     1561 gcccaggcat cgcagctctc gtccagcccc cggctcaccc ggaagaggag agacatatag
     1621 cagaggaccc cggccaccgc tccgattacc agggctccaa gcacgtccac aaagccagcg
     1681 gcaggggtta tggccaccag gccggctatg ccgccgctca ccattcccag ggcgctgggc
     1741 ttgcccttca gccagctgga gaggagccag gtcagagcac cggcagcagc ggaggtattg
     1801 gtcaccacca gggcggaggc agcaaggccg ttcgctccca gagcggagcc ggcattgaag
     1861 ccaaaccagc caaaccacag cagtccggcg cccagcatgg ccatggggat attatggggc
     1921 tccatgacat aggagccgaa gcccaccctc tttccaatca ccatcgctat ggccagggcg
     1981 gagaagccgg agcttatatg gaccaccgtc ccaccggcaa agtccagggc tcccatctga
     2041 gccagccagc cacctgccca gacccagtgg gccaggggat catagaccag ggtggtccag
     2101 agcagagcga gcacgatgaa ggagccaagg cgcaccctct ccgccaccgc tgaggtgagg
     2161 atggccaggg tcactgcagc gaataccatc tggaaggcga cgtagagcag atggggcaga
     2221 ttttccgcca gtggcgcttc gcccaggcct atgccattca aggccaggta atccagagca
     2281 cctattgctc cgcctatatc ctggccgaat gataggctgt agcccaggaa aacccactga
     2341 acgctggcca gggcaaaggc caggaaggag aggccaatca tcgataccac agtcttcttg
     2401 cgcaccaggc cgccgtagaa taggcccacc cccggggtca tgagcatgac catggcggtt
     2461 gagaccagga cccaggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     2581 aagaagcatc tcggagaagg atcagacttt cgcatcgctg atgtcatgga gacaatggaa
     2641 ggcatcgcac cgtttctgga taaagaggtc ataattggat tgaaatgcaa aaactgcgat
     2701 agctatgcac gaggctcatt tcccctgagc aacgactttt gggcggtcga cgaaaatgag
     2761 cactgccatg agaagaagac ctctctgaag aacctgaatg ccttgaagct ggccggctgc
     2821 catcaatact catgtcagga aagggaggag ctgatgaagc tgggccaaga tattctggac
     2881 gaggatctca ggcctggatt cagcgataga aagctatatg ataggagcaa gttcgccagc
     2941 tacagcgtag acttccgcat gctctccaat ccgggaaata tctgcatcga gtttgggcat
     3001 cttacggatg gagggttcac ctgcagcgat atcaggcttt acatcataaa gaggctcgat
     3061 ccgggatttg gagaggaggc ctctcgcaag atatctgagg ctttgaagaa tggtcccgtc
     3121 atggcgcttg aggccaacct gcagatcaga aggcagcttg atattgacta tgtcctggag
     3181 tggattgcca aaaagcgaaa gatggatata gtatatctct agctctattt ttgatttaga
     3241 caataattga taattaaagg atgttttcga caatggcgca accgctagtg ccagatacca
     3301 gcgtggtaat agatggcaga gtctctgcca ggatcaagtc tggagagctc cagggcagga
     3361 gaattgtagt gcccgaggct gtggtggcgg agctggaagc ccaggccaac cacggccgag
     3421 agatcggcct gaagggattg gaggagctga gaaagctctc tgagctggct aaagccggca
     3481 ggatcgagct ggagtatgta ggaataaggc cgaacctgga tcagatcaag ctcgccggtg
     3541 gtggggagat cgatgctatg atcagggatg ttgccctgga gctgggcgcc tccttcctca
     3601 ccagcgatca ggttcaatcc cgggtggcag aggcgatggg gctggatgtg gagtacgtcc
     3661 gtccccaaag ggaggcgatc aagcctctgt ccctggagag atacttcacc gaggataccc
     3721 tatccattca cctcaaggag agcgcaatac ccatggccaa gaggggccat gtaggggact
     3781 tctccctggt ccggctgggc gaaaggccct catcggagag ggagctgagg gacctatccc
     3841 gggagatata cgaacgggct aaagccgatc cagatggcta tgtggagatg gaaaagatcg
     3901 gggcagcgat aatccagatt gggccgatga gaatcgccat cgccaagccg ccgttctccg
     3961 acggcctgga gatcaccgcc gttcgaccgg tggccaaggt gagctttgac tcctaccgcc
     4021 atagcagcat gctcaaaacc cgcctcaagg aagggcagag gggaattcta atcgccggtc
     4081 cgccagggtc gggcaagtcc accttcgcag ccggattggc cgaatacctt ctggactgca
     4141 actatgtggt caagactctt gaatctcctc gcgatctgca ggttcccccg gagatcaccc
     4201 agtacagccc tctggagggg agcatggagg catcggcaga tatactgctc ctggtcaggc
     4261 ccgactatac catctacgat gaggtcagga agacgcgcga cttccaggtc tttgccgata
     4321 tgaggcttgc cggggtgggc atgatcggcg tggtccatgc caacaaaccc atcgatgccc
     4381 tgcagaggct ccttggccgg gtagagctgg gcatgatacc tcaggtggtg gacaccgtgg
     4441 tcttcatcga gagaggggag gtcacgaagg ttttggatgt ggagtttgtg gtcaaggttc
     4501 cctcgggaat ggtggaggct gatttagccc ggccggtgat agtggtcaag gacttcgaga
     4561 gcggccaggc ggagtacgag gtgtactcct acggagagga gatcgtggtg atgcccgtca
     4621 ccagagagga gagaaagccc tcctggaatc tggccagcag ggagatcgag aaggagatct
     4681 ctcaccatgt gcgtgggccc ttcaaggtgg agatgctcac cgactccagc gcagtgatcc
     4741 atgtacctca agaggatgct gcgagggtga tcggcaaggc gggaaagaac atcgaccaga
     4801 tcgagaggag cctggggatg catatcgatg tccgggcaag ggacgagccc tctgttcctg
     4861 agccgaggat ggaggagacg gcaaagcata tcatcctctg gctggacggc atggccggtg
     4921 agtcagtcga tgtctctgta ggggatgagc ccgccttcac cgccactgtg ggccgaagag
     4981 gagatatccg catagccaaa tcctcggaga tggccaagag gatcataaag aagatgaaaa
     5041 agggtgagag tatagaggtg cataggtcgg gatcggaatg ggatggatga gatatccggt
     5101 cagcatcctc atcctcctct tcctggcagc ggtgctcact cttagcgcca gcgcccagga
     5161 tcccgaaccc tggcaggacc agagaggcaa tgccacccct gttctgatgg gaaagaatgt
     5221 agaggaagca gaagtaggcc aggaggctac cgggctaaaa tcccggctct tgagccccat
     5281 ggtccgatgg gagctggatc tgaaagatag ccttgatagg agtgtggaaa tggagatgta
     5341 ccaatcgaac gatgccctat tcggcaaggg caggatcatg gcggacggaa tagagcagaa
     5401 cgtctctgcc agcggcacaa tctcaggaaa caagctggat c
//
LOCUS       DAOSGP010000073         5426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13341_length_5426_cov_13.447030, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000073 DAOSGP010000000
VERSION     DAOSGP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13341_length_5426_cov_13.447030"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLV49_03335"
     CDS             <1..1335
                     /locus_tag="PLV49_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG97909.1"
                     /translation="LLINPPGWVGDRIPLGYDLVPSVPLGLGYLASTMESEGITAKIV
                     DMDWGGFSKEELVDMISKFDPDVIGITSFTSNYANAVDIAKAAKSHNPNIKIVFGGVH
                     ATFIHRDVLHTIPEVDIVVRYEGERAICEICDVLDRGRKLENVKGISFRHDNRVFSTP
                     LRHRIEDLDSIPFPAFHLLEPSIEHYIGEGKEKGFPIITTRGCPFNCIFCSTAALHGR
                     KYRTRSANNVLDEMEFVQDKYKINVISFADDNFTMKRNRVIDICRGIKERNLDFEWGC
                     SARVDLLSKDLLEVMNSSGCKNIFFGIESASQRVLDTVKKGFSIEKAKEMVKLTEKMG
                     IKTHCSFILGLPNETVESIDEMIRFVEETMPSGRVLPNVLEILPGTELWDKESEYFGN
                     TPSIPAADIIRVQLELFYKFFEISAKGRELLTIEPPDIEIIGGSTAFAQTQM"
     gene            complement(1556..1906)
                     /locus_tag="PLV49_03340"
     CDS             complement(1556..1906)
                     /locus_tag="PLV49_03340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOG97910.1"
                     /translation="MPKGSIVLVHFPFTDLSSTKLRPALVLHEGHLDVTIAPMTTQRP
                     DPPLPSDLLIRKGTLSFKATGLKEESWLLIDKIATLEKMFIEGILGKADDDLKRDVNA
                     KLAECLMFNDVSAI"
     gene            2278..2706
                     /locus_tag="PLV49_03345"
     CDS             2278..2706
                     /locus_tag="PLV49_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97911.1"
                     /translation="MDPLTLAVIAAPFVAKGAEAFSSSAGDKLGAKVGELCQAVIDKF
                     RGDSDAEETLALAQKRPDSKGQQSALEEILAEKLKDDQDFAAKVQRLVDDLQKGGGGR
                     TAFDQRGQTVHGSQTNIAGNVNRPVLSGNFSGPVNTDKSK"
     gene            2716..4872
                     /locus_tag="PLV49_03350"
     CDS             2716..4872
                     /locus_tag="PLV49_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG97912.1"
                     /translation="MPDNKSRFDQRGQKVISQQINVAGDATLPPPPLVLVPHLPAPPR
                     DFTGRDEELKDLLADFDRGATITGLRGMGGIGKTALAYELAEKLAGRYPDGQILVELR
                     GTGPEPMTPAEAMAKVVRIYYPEAKLPGSEAELARIYHSLLHDLRVLMLLDNAFNDRQ
                     VRPLLPPAGCGVIVTSRRKFTLPGLVPMDLDVLPEDKAVELLLRLWNPEASPSTEQLK
                     DKDLSRIACLCGYLPLALRAAGSLLANSPDLSPAQLARDLRDERTRLKRIGKEGVDLD
                     VESSFGLSYSRLTEETARVFLLLSVFPAGFDAAAEEVVCRDEGHRHLRELMRWSLVEY
                     QSLGVEYGRYHLHDIVRIYAASCLQAGEMGEAEERHAEHYWRVLSASNEQYKQGGENL
                     LAGLALFDRERANILAGWSWAERNLKDSRAADSLCSSYPGAGAYVLNLRLHPQEQIRW
                     LETAADAAKQMQDRGAEGAHFGNIGLAYADRGETGKAIVYYQQALTITREIGNRQDEG
                     AILGNLGRAYANLGEPRKAIEHHDQALTIFKEIGDRRDEGTTLCNIGIAYAALGKIHN
                     AIEYFEQALAIAREIEERRNEGICLGCLGQANINLGEIRKAIDHYQQALAIAREIGDR
                     RSEGNSLGSLGTAYKNLGEPHKAIEHYDQARTIFREIGDWRGEGNSIWNMSLVLNGLG
                     NRAEAVKLAGEALAIYEQIKSPIAERVRLQLAEWQR"
     gene            complement(5126..5311)
                     /locus_tag="PLV49_03355"
     CDS             complement(5126..5311)
                     /locus_tag="PLV49_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOG97913.1"
                     /translation="MKFNIIIEKDNDGRYVAECPDLPGCFSEGESLEDALENVIEAIS
                     GCQKSRLKDAARRLKIA"
BASE COUNT         1409 a         1316 c         1468 g         1233 t
ORIGIN      
        1 ctgctcatta atccccctgg ttgggttgga gatagaatcc cgttaggtta cgatctagtg
       61 ccatcggtgc ctttgggttt gggctacctt gcatcaacta tggaatccga aggcataact
      121 gctaagatcg ttgacatgga ttggggcggt ttttccaaag aggaattagt cgatatgata
      181 tcgaaattcg atcctgatgt cataggaata acatccttca cctccaatta tgcaaatgct
      241 gtggacattg ccaaagcagc aaaatcgcac aatcctaata tcaaaatagt attcggtggc
      301 gtacatgcta cattcatcca tcgagatgtg ttgcacacga ttcctgaagt ggacatagtt
      361 gttcgatatg aaggagagcg agcgatatgc gaaatctgtg atgttctgga taggggaaga
      421 aagctagaaa atgtcaaagg aatcagcttt cgccatgata atcgggtatt ttccacacca
      481 cttaggcata ggatagagga ccttgatagc ataccgttcc ccgcttttca tctattggag
      541 ccctcgattg agcattacat aggggaggga aaagagaagg gctttcccat aataactact
      601 cgaggatgcc ccttcaattg catattttgt tccacagcag cacttcacgg tcgcaaatac
      661 cgtacaagaa gcgcaaataa tgtcttagat gagatggaat ttgtacaaga taaatacaaa
      721 ataaatgtaa tcagctttgc agatgataat ttcaccatga aacgaaatag agttattgac
      781 atatgcagag gcattaaaga aagaaatctc gattttgaat ggggttgcag cgccagggtg
      841 gacctattat caaaggattt attggaggtg atgaattcct ctggctgcaa aaatatattt
      901 ttcggtatag aatccgcatc tcaaagggta cttgatacgg ttaagaaggg ctttagcatt
      961 gagaaagcaa aggagatggt aaagctcact gagaaaatgg gaataaaaac acattgttca
     1021 tttatcctag gcttgccgaa cgagaccgtg gaatcgatag acgagatgat cagatttgtt
     1081 gaagagacta tgccatcggg aagagtgctt cctaacgttt tggaaatact tcccggcacc
     1141 gagctatggg ataaagagag cgaatatttt ggaaatacgc catccatacc agctgcagac
     1201 ataatcagag ttcaattgga gcttttctac aaattctttg agataagtgc aaaagggagg
     1261 gaattgctta cgattgagcc accagatatt gagatcattg gcggatcgac tgcatttgcg
     1321 cagacgcaaa tgtaaatgga tataatccaa taatagttct aataatattt ttatatagtt
     1381 aatgtgataa ttgcgggccg gccccgcagg cttgttcaag agcagaagag gcgctggcca
     1441 tcttcgagca gatcgagagc tcctgcgcgg agcgtgtgcg gaggcagctg gcggaatggc
     1501 cgggataagg tggcaagggc atgtccaggg actatcccgc tctataatta tggccctaga
     1561 tagcagaaac gtcgttgaac atcaagcatt cagccaattt cgcatttaca tctcgtttga
     1621 gatcatcatc tgctttcccg agtatgccct caatgaacat cttctccagg gtcgctatct
     1681 tgtcgattag gagccaagac tcctctttca atcctgttgc tttgaaggag agcgtgcctt
     1741 tgcgtattaa caaatccgat ggcaatggag gatctggacg ttgtgtagtc atgggggcta
     1801 tggtcacatc caaatgtcct tcatgaagga ctaaagcagg cctcagcttt gtggaactga
     1861 gatctgtaaa aggaaagtga actaaaacta tgcttccctt aggcattctg ggaacttgca
     1921 tgctacctga atctgacttt aagatcctct acggtataaa tatcaggctc attttcgaga
     1981 agcgaggcta aagccctctc ggatagtctc ataaggcctc tggtaattgc tctgtcttcg
     2041 ccaagctccg acagatcgca aggaagttgc gaggtctccc ctgaactctc acacatagat
     2101 actacttgtg gattcttgat atttaaaaca tgggtggaaa atagcctgct atcgagagtt
     2161 gagcgagttc ctggacctga gccggtcaga gctacccaga tcatcctacc tgagcatcaa
     2221 atccagaaag gcttatcatt tcagccctct caacacacca tcaaggatgt gatgcagatg
     2281 gatccattaa cgctggccgt catagccgcc ccgtttgtgg ccaaaggggc ggaagccttc
     2341 tccagctcgg caggcgataa gctgggcgca aaggtcgggg agctatgtca ggcggtgata
     2401 gacaagttca ggggcgactc cgatgcagag gaaactctgg ccctcgccca gaagaggccg
     2461 gactccaaag ggcagcagag tgcgctcgaa gagattctgg cggagaagct aaaggacgac
     2521 caggatttcg cagcaaaggt gcagaggctg gtggacgatc tgcagaaagg aggcggcggc
     2581 aggacggcct tcgaccagcg gggccagact gtgcacggct cgcagaccaa tattgcggga
     2641 aatgtcaaca ggcctgttct ctctggaaac ttcagcggcc cggtgaacac ggacaaatcc
     2701 aagtaaaagc gaaccatgcc agataataaa tcccgtttcg accagcgtgg ccagaaggtg
     2761 atcagccagc agatcaatgt ggcaggggac gctactctcc cgcctcctcc tctggtcctc
     2821 gtccctcacc tgcccgctcc accaagggac ttcaccggcc gggacgagga gctaaaagac
     2881 cttctggcag actttgatcg gggagccacc atcaccggcc tgcgtggcat gggaggcatc
     2941 ggcaagacgg ctctagctta cgaattagcc gagaagcttg ccgggcgcta tcctgacggc
     3001 cagatcctgg tcgagctgag gggcacgggc ccggagccca tgaccccagc cgaggccatg
     3061 gccaaggttg tgaggatcta ttatccggag gctaagctgc cagggagcga ggcggagttg
     3121 gccaggatct accactctct ccttcacgac ttgcgtgtgt tgatgctgtt ggacaatgcc
     3181 tttaacgacc gacaggtgcg tcctcttttg ccgccagcgg gctgcggagt gattgtaacc
     3241 tcccgccgca agttcaccct ccccggtctg gtgccaatgg acctggatgt cctgccggag
     3301 gataaggcgg tggagcttct gctgagatta tggaatcccg aggcatcgcc atccacagaa
     3361 caacttaaag acaaagatct gagcagaatt gcatgcctct gcggctacct gcctctagcg
     3421 ctgagggcag cgggaagctt gctggccaac agccctgacc tgagcccggc tcagctagcc
     3481 cgagatctgc gggacgagcg cacccggctg aagcgcatcg gcaaggaggg cgtggacctg
     3541 gatgttgaat ccagcttcgg cctgagctac agccgtctga ctgaagagac cgcccgcgtt
     3601 tttctcctgc tctccgtctt ccctgcgggc ttcgatgccg ctgcagaaga ggtggtctgc
     3661 cgggacgagg gtcaccggca cctgagagag ctgatgcgct ggagcctggt tgagtaccaa
     3721 tcactgggcg tagagtacgg gagatatcac ctgcatgaca tagtgcgaat ctatgccgcc
     3781 tcctgcttgc aagccgggga gatgggggag gcggaagagc gccatgctga acactactgg
     3841 agagtactct ctgcttccaa tgagcaatac aagcagggtg gggagaacct cctagcaggt
     3901 ctggcgcttt tcgatcggga acgagccaac atcctggcag gatggagctg ggcggagagg
     3961 aaccttaagg atagccgtgc tgccgactcg ctctgcagct cctacccagg tgcaggagca
     4021 tatgtgctga atctgcgcct gcatcctcag gaacaaattc gttggctgga gacggccgct
     4081 gatgcggcaa aacaaatgca ggatcgaggc gctgagggcg cacactttgg taacataggc
     4141 ttggcttatg ccgaccgagg cgagacggga aaagccatcg tctactacca gcaggccctg
     4201 accatcacca gggagatagg gaaccgacag gacgaaggtg ctatactagg caacctggga
     4261 cgggcctacg ccaatctagg agagccgcgt aaagctatcg aacatcacga tcaggcactg
     4321 accatattta aagagatagg tgaccggcgg gacgaaggta ctacgttgtg caatataggc
     4381 attgcctatg ccgctttggg caaaatccac aatgccatag agtacttcga gcaggcttta
     4441 gccattgcta gggagattga ggaacgaagg aatgaaggaa tatgcctagg ctgcctgggc
     4501 caagccaata tcaatctagg cgagatccga aaagccatcg accactacca acaggctctt
     4561 gccattgcca gggagatcgg agatcgacgg agcgaaggaa attcattggg cagcctgggc
     4621 acagcttata aaaatttagg ggaaccgcac aaagccatcg agcactacga tcaggctcgg
     4681 accattttta gagagatcgg ggattggagg ggcgaaggaa actccatctg gaacatgagc
     4741 ttggtcctga atggcctagg gaatcgagcc gaggccgtga agctagcagg agaggcgttg
     4801 gccatctacg agcagatcaa gagcccgatt gcggagcgtg tgcggctgca actcgcggag
     4861 tggcagaggt agaaaagtct cgtaaagagc tctttcatgc aggaggagcc atccctgaga
     4921 cagggcaacc actttttcat ttattgcaga agcaagctac atactgttcg tttgtgaatc
     4981 ctgccttttt atcagccttt taagaaggcc aactccaaga tccctatctc tatgaacagg
     5041 cattaacaaa tggatgtcgc ttccttcttt gatagagatt tagtgactgc cttcaatatg
     5101 atttattgtc cagccagcga tcgaattatg ctatcttaag ccgcctcgct gcgtccttaa
     5161 gccgggattt ctggcatccg cttattgcct cgataacgtt ttccagagca tcctcaaggc
     5221 tctctccttc agaaaagcag cctggaaggt cgggacactc ggctacatat ctgccgtcat
     5281 tgtccttctc tatgataata ttaaatttca tttcgcccgg aattcgccct atttccccag
     5341 tatcccctta tgcgcctccc tgaatccggg gtccctcatc tggcaggagc acctcccctt
     5401 gaaccaggga tagcgggtca tctctc
//
LOCUS       DAOSGP010000074         5411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13397_length_5411_cov_18.569828, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000074 DAOSGP010000000
VERSION     DAOSGP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13397_length_5411_cov_18.569828"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /gene="thiL"
                     /locus_tag="PLV49_03360"
     CDS             complement(<1..926)
                     /gene="thiL"
                     /locus_tag="PLV49_03360"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOG97914.1"
                     /translation="MKGAIGERDLIRRISVILGGLENDDCAVIDAGERYLVASTDMLH
                     QKTDFPDIMNPWQMGWMAVAVNLSDIAAMGAEPAGVLIAAGLPPEADQYFIDELFSGF
                     GDCAALYGTRILGGDTDSHLELTITGTSLGFVEKELILRRRGARAGDLLCTTGSLGAA
                     GGGLWAWQNGAKSELITSLLEPEPRLKEGRALAKSGSVTAMMDNSDGLALSLTDLSEV
                     SGVGFLVYQDKLPLAPGLVEMVGQENAIDMALSAGGDYELVFTLRSSGLEAARRACDL
                     TVIGEVVEEGIWMERAGKRRQIERLGYEHRIGL"
     gene            1185..>1705
                     /locus_tag="PLV49_03365"
     CDS             1185..>1705
                     /locus_tag="PLV49_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97915.1"
                     /translation="MESGIYANPFIMGRPVNLQGRDTGSGNPILNPEKGRAILAAQGA
                     VLSGFDFSSARAGDERSAGCRLEVVLPATIYLNDFPGKNSVCPEDVATWNSSRMISYQ
                     YNSRVQRSFLGNYWADYAGEDKNGDGIGDEPVVLNQDNIDNYPLMQPAESYLISDEAD
                     ALGKSEMELLDARV"
     assembly_gap    1706..1805
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1872..3167
                     /locus_tag="PLV49_03370"
     CDS             1872..3167
                     /locus_tag="PLV49_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HOG97916.1"
                     /translation="MAKSDIKSEDKSDQKTEAKAPASKKPEVNIGMVGHVDHGKTTLV
                     RALSGSWTDQHSEEVKRGISIRLGYADATFRKCPSCPDPDAFTVEEKCPHCGADTEIL
                     RTVSFVDSPGHETLMATMLCGAAIMDGAVLVISANEPCPQPQTKEHLMALDITGIDRI
                     VIVQNKIDLVSREEVMEHYQQIKDFIKGTVAEKAPIVPISAQQNLNVDLVIKAIEEYI
                     PTPHRDIEKPALLKIARSFDVNRPGASPDALKGGVIGGSLNQGVLRAGDKIEICPGRL
                     VDYEGRKQWVPIQTKVLTLLAGSQSQEEITPGGLIGVGTLLDPIMTKSDALVGQVAGE
                     PGKLPPIRNAFTMNMQLLERVVGVTDESSVEPIHSSEPLMLNVGTATTVGVVTSAREG
                     GVVQVQLKRPVCADKGDRVAVSRRIGARWRLIGVGSIID"
     gene            3177..3584
                     /locus_tag="PLV49_03375"
     CDS             3177..3584
                     /locus_tag="PLV49_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOG97917.1"
                     /translation="MKQIDADSVKVIIDTNALMTAEQFGVDIFSELLRLGYIEWLVPA
                     QVKGELRSLADGADKGRDKTAARVALGLAERCTIVGIDNCPADQAIEELAEKEGAAVF
                     TNDKALKKRLFSKGITVIYLRQGRYLEAMKKEY"
     gene            3584..4168
                     /locus_tag="PLV49_03380"
     CDS             3584..4168
                     /locus_tag="PLV49_03380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HOG97918.1"
                     /translation="MYKKMKLKGVARIEPDHLGDPLEAAVERALRDKYEGVVDKSLGT
                     IVAVLGADDIGEGHILAGDGSIYYEVNFDTLVFKPEMQEIIEGEVVEIVKFGAFLSLG
                     PFDGLLHVSQITNEYISYDEKNARLVSKESNKSLGEGDRVRARVIAVSLNEKEPRESK
                     IGLTMRQTGLGKLEWLDAADETEKENASVQAEAK"
     gene            4165..4350
                     /gene="spt4"
                     /locus_tag="PLV49_03385"
     CDS             4165..4350
                     /gene="spt4"
                     /locus_tag="PLV49_03385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HOG97919.1"
                     /translation="MTEQACRECRRIVEGQVCPICNSASLSKDWSGYVVIIDPKESII
                     AEKLEIKLPGKYALKVR"
     gene            4388..4882
                     /locus_tag="PLV49_03390"
     CDS             4388..4882
                     /locus_tag="PLV49_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HOG97920.1"
                     /translation="MLKSPLGKLCKGNGTECVEAMRDELQGAKKVAAIGDMTAFYLLK
                     SSIMPDLAVVDNKTKRSQLPDHILQSLEHNSYKRVEVKNPPATLTEELIDQIKDSLAG
                     EERVKIVVEGEEDLATLPAILYAPPGSVVIYGQPDEGSVLVRATPEMKMYIDELMKKM
                     IVEE"
     gene            4879..5193
                     /gene="rps24e"
                     /locus_tag="PLV49_03395"
     CDS             4879..5193
                     /gene="rps24e"
                     /locus_tag="PLV49_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S24e"
                     /protein_id="HOG97921.1"
                     /translation="MKLEIKVIVEKNNPLLKRREIVFKVIHDESTPSRKSVVERLAAT
                     MNSKVGLVYVDGLKTEFGKRETQGYAKIYETAERAEQVERAHIIERNTFTKPEEAKPE
                     AS"
     gene            5194..5376
                     /locus_tag="PLV49_03400"
     CDS             5194..5376
                     /locus_tag="PLV49_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HOG97922.1"
                     /translation="MAAKKGKTGEVKGVSKYYELSGDTIKSRKSVCPRCGNGVFLADH
                     GDRQSCGKCGYTEFKK"
BASE COUNT         1350 a         1368 c         1515 g         1078 t
ORIGIN      
        1 agccctatcc gatgctcata gcccaatctt tcgatctgtc ttctcttccc tgccctttcc
       61 atccagattc cctcctccac cacctcgcct ataaccgtca gatcgcaagc tcttcgggcc
      121 gcctccaggc ccgaggagcg cagggtgaat accagctcat agtcccctcc cgcgctcaat
      181 gccatatcta tagcattctc ctgccccacc atctccacca agccgggagc caggggcagc
      241 ttatcctgat agaccagaaa gcccactccg ctcacctcag aaagatcggt gagggacaga
      301 gccaggccat cgctgttgtc catcattgcc gtaacgcttc cgctcttcgc cagggcccgg
      361 ccctccttca gccggggctc gggctccagc agactggtta taagctcgct tttcgcgccg
      421 ttctgccagg cccacagccc gcctccggct gcccccagag agccggtggt gcagagcaag
      481 tcccccgctc gtgctccccg gcgacgcaga atcagctcct tttccacaaa gcccaaagat
      541 gtgccagtga tggtcaattc cagatggcta tcggtgtccc ctcccagaat tcgggtacca
      601 tagagagcgg cacaatcgcc aaatcctgaa aatagctcat cgataaaata ctgatccgcc
      661 tccggcggga gtccggcagc gatgagaact cccgcgggct ctgcccccat ggcagcaata
      721 tcgctcaggt tgactgctac cgccatccag cccatctgcc aggggttcat gatatcagga
      781 aagtcggtct tctggtggag catgtcagtg cttgccacaa gataacgctc tcccgcatcg
      841 ataactgcgc agtcgtcgtt ctccagccca cccaggatca cggagatacg ccggatcaga
      901 tccctctcgc caatggcccc tttcattctt tcttgctccc tctaattgta tagatcgcct
      961 cagctccgaa agttattaag attcaaagat gcattaattg tgatatgctc gattacaaaa
     1021 ggttgttcgt ccaggcatca attgccctcc tgctttccct catgctttct agctcgcttt
     1081 ctggagctgg agcagcccag atgacgagga tctgtccaca aggctgcagc aatctgagca
     1141 tccaggaggc acttgttaat gcctctgctg gagatacgat cattgtggaa agcggaatat
     1201 acgctaatcc cttcataatg ggccggccgg tgaacctgca gggcagggac acaggcagtg
     1261 gaaatccgat cctcaatccg gagaaaggaa gagctatcct ggccgctcaa ggagcggtgc
     1321 tgtcgggatt cgacttttcc agtgctcgtg ccggcgatga aaggagcgca ggctgcagac
     1381 tggaggtcgt ccttcccgct accatatacc tgaacgattt tccgggcaaa aacagcgtct
     1441 gccctgagga tgttgccacc tggaactcga gccggatgat cagctaccag tataacagcc
     1501 gggtacagcg cagttttttg ggcaactact gggctgacta tgccggcgag gataagaacg
     1561 gcgatggaat cggcgatgag ccggtggttt taaatcagga caatatcgac aactatccct
     1621 tgatgcagcc ggcggaaagt tatctcattt ccgatgaggc cgatgctctt gggaaaagtg
     1681 agatggagct tttggatgct cgagtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnncatga actatttata cggtgatgcc catcaagccc tgatcattac aatccttaag
     1861 gagatctcaa tttggcaaaa tccgatatca aatctgagga caaatcagat caaaaaacag
     1921 aggcgaaggc gcctgcctcc aaaaagccgg aggtcaatat cggcatggtt ggacatgtcg
     1981 atcacggcaa aaccactctt gtccgagcgc tatccggaag ctggaccgat cagcatagcg
     2041 aggaggtcaa gagaggaatc tccatccgtc tgggctatgc agatgccacc ttcagaaagt
     2101 gccccagctg cccagatcca gatgctttta ccgtagaaga aaagtgcccc cactgcggtg
     2161 ctgatacaga gatattgcgc accgtctcct ttgtagattc tccggggcac gagaccctca
     2221 tggccaccat gctctgcgga gcggcgatca tggatggcgc agtactggtc atctccgcca
     2281 atgaaccctg ccctcagccg cagacaaaag agcatctcat ggccctggac atcacaggca
     2341 tcgacagaat tgtgatagtg cagaacaaga tcgatctggt atccagggaa gaggtgatgg
     2401 agcactacca gcagatcaag gatttcataa agggcaccgt agccgagaag gcgcccatag
     2461 tgcccatctc cgctcagcag aacctgaatg tcgatctggt catcaaggcc atagaggaat
     2521 acatacctac tccccataga gatatcgaga agcctgctct gctcaagata gcccgatctt
     2581 tcgacgtcaa ccgtcccgga gcctcccctg atgctctcaa aggcggggtc attggcggct
     2641 ccctaaacca gggtgtgctt cgtgcaggcg ataagatcga gatctgcccg ggcaggctgg
     2701 tggattacga ggggcggaaa cagtgggttc ccattcagac caaggtgctg accctcctgg
     2761 caggaagcca gagccaggaa gagatcacac ccggcggcct gatcggcgtg ggcaccctgc
     2821 tggaccccat aatgaccaag agcgatgctc tggtgggcca ggtggcaggt gaaccgggca
     2881 agctccctcc catcaggaac gcattcacca tgaacatgca gctcctggag agggtggtag
     2941 gagtgaccga tgagtccagc gtcgagccca tacattccag cgagcccctg atgcttaacg
     3001 tgggaactgc caccactgta ggggtggtga ccagcgcccg ggagggaggc gtggtccagg
     3061 ttcagctaaa gaggccggtc tgtgccgata aaggcgatcg tgtggctgtc agcaggcgca
     3121 ttggcgccag gtggcggctc attggcgtcg gctccataat cgactgaaga aataatatga
     3181 agcaaatcga tgccgatagc gtaaaggtga taatcgacac caacgccttg atgacggcag
     3241 agcagttcgg agtggacatc ttcagcgagc tattgcggct gggatacatt gagtggcttg
     3301 tgccggccca ggtgaaagga gagctgcgct ccttggcaga tggggcagat aaagggcggg
     3361 acaagaccgc ggccagggtg gccctgggcc tggctgaacg atgcacaatc gttggcattg
     3421 acaactgccc tgctgatcag gccattgagg aactggcaga gaaagagggg gcagcagtct
     3481 tcaccaacga caaagccctg aagaaaagat tatttagtaa aggcatcacc gtaatatacc
     3541 tgcgccaggg tcggtaccta gaggcgatga agaaggagta ttgatgtaca agaagatgaa
     3601 gcttaagggt gtggccagga tagagccgga tcatctggga gatcccctcg aagcggcagt
     3661 ggaaagggca ctgcgggata aatatgaggg ggttgtggac aagtctctgg ggaccatcgt
     3721 ggccgtgctc ggagcggatg atataggtga aggccatatc ctggcgggag acggatccat
     3781 ttactacgag gtcaacttcg ataccctcgt cttcaagccc gagatgcagg agatcattga
     3841 gggcgaggtg gtggagattg tgaagtttgg cgcatttctc tctctggggc cgttcgacgg
     3901 ccttctgcac gtcagccaga taaccaatga gtatatatct tatgatgaga agaacgcgcg
     3961 cctggtcagc aaagagtcca ataaatcctt aggagaaggg gatcgggttc gagccagagt
     4021 aatagcggtt agcctgaatg agaaagagcc tagagagagc aagatcggac tgaccatgag
     4081 gcagacgggc ctggggaagc tggaatggct tgatgctgca gatgagacag agaaggagaa
     4141 tgcgagcgtc caggcagagg caaaatgact gaacaggcat gcagagaatg tcgcagaata
     4201 gtagaggggc aggtctgtcc catatgcaat tcagcctcgc taagcaagga ctggagcgga
     4261 tatgtggtta tcatcgatcc caaggaatcc atcatagcgg agaagctgga gatcaagctt
     4321 cccggcaaat acgctttgaa ggtgcgatag attgcgtaca cttcttctgc cggaagattt
     4381 gcggctattg ctcaagagtc ctctgggcaa gttatgcaag ggcaacggga ccgaatgcgt
     4441 agaggctatg agagatgaat tacagggggc taaaaaggtg gccgcgattg gcgacatgac
     4501 cgcattttat ttgttgaagt catcaataat gccggacctg gcggttgtag acaacaagac
     4561 caagaggtcc cagcttccag atcacattct tcaaagcctc gagcacaata gctataagag
     4621 ggttgaggtg aaaaatccgc ccgccactct gaccgaagaa ctaatagacc agatcaagga
     4681 ttctcttgcc ggagaggagc gggtgaagat tgtggtagag ggtgaggagg atctagcgac
     4741 ccttcctgca attctctatg ccccacctgg ctctgtggtg atttatgggc agcccgatga
     4801 gggaagcgta ctggtcaggg caactcctga aatgaagatg tatattgatg aattaatgaa
     4861 gaaaatgatt gtggaggaat gaaattggag ataaaggtta ttgttgagaa gaacaatccc
     4921 ttgctgaagc gcagggagat agtcttcaag gttattcacg atgagtccac gccatccaga
     4981 aagagcgtgg tggagaggct ggcggcgacc atgaactcca aggttggctt agtctatgtc
     5041 gacggcctta agaccgagtt cggaaagcgc gagacccagg gatatgccaa gatctatgaa
     5101 acggctgaga gggcagaaca ggtggagcga gcccatatca tagagcgaaa cacgttcacc
     5161 aagcccgagg aagccaagcc ggaggcaagc taaatggcag caaagaaggg caagaccggt
     5221 gaggtgaagg gcgtatccaa atattacgag cttagcggag acaccatcaa atccagaaag
     5281 tcggtctgcc cgagatgcgg caatggcgtc ttcctggcgg accatggcga cagacagagc
     5341 tgcggcaaat gcggctatac tgagttcaag aaataatttc cggaatccag gtctcgattc
     5401 cggacctgat t
//
LOCUS       DAOSGP010000075         5391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13462_length_5391_cov_18.313906, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000075 DAOSGP010000000
VERSION     DAOSGP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13462_length_5391_cov_18.313906"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLV49_03405"
     CDS             complement(<1..841)
                     /locus_tag="PLV49_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOG97923.1"
                     /translation="MYLDYLPYESLRPHQDRMLDAVYDVVSRGKQGILMIDAPTGTGK
                     TSCISAALAAAPGKIVVAVRTVSQIDIYIDEINRIWSKTRHKPEIAYMVGKQRICPLE
                     GEFRAESIYAGCLRLREWTKNYVSSKIGKGNAAIYDPYADSIPEEEPGYRTFCPYYLR
                     SREGFEINGTVHFRRSARALDVVEGLKKRVTAPSGLMDSCQGICPYEIMSLYAKNSDI
                     VIMNYSHLFSPDFQDIIFQWLEMDSEKVTLIIDEAHNLGDAVRAMNSRHLTQRMIDLA
                     ETEV"
     gene            1117..1623
                     /locus_tag="PLV49_03410"
     CDS             1117..1623
                     /locus_tag="PLV49_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97924.1"
                     /translation="MSLDTKRLEERMAGALVDMMSKSQSDGEGKGGNGDSISSGLKGP
                     NPLNPELPGTNHSVAGTSVLNSSIANISALNFSTTNASINSSATNGSATDAFAGNQSR
                     LNSSIPAPGGSTGNQGIGSSSNAKFDGYYGIKSSQHQMGENNIDSSTFLSGSFSMEKA
                     VKFQDIGI"
     gene            1640..2950
                     /locus_tag="PLV49_03415"
     CDS             1640..2950
                     /locus_tag="PLV49_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97925.1"
                     /translation="MKKTNIRLGTASRRFQSILLVLPLLASLLFVFLISFYHISPAAA
                     SLYSGELTEEWNRTYGGRYGDGAWSVQETNDGGYVLVGHTATRGEGSDLWLVRVDPDG
                     NPLWSRIMGGSGEDVGYFVRETADRGYIIAGSTNSFGLGEERLWLIRMDGNGSLLWDK
                     TFGGFVHSSGDGGWSVDETDDGGYIATGYTQSKSIGRKDLWLIRTDSNGNLLWDRSYG
                     GADEDVGLSVLQSQDGGFIVAGRTASLGKRGDDIWLLKTDDQGVMSWNSSFGGDKDDV
                     AFQVAELANGYALVGRSESGLEEEKIILIRLDQLGRKIWERSYLGSSGTSLQQTMDGG
                     FVIGGRIDNADSGRDALIIKTNSAGLEEWRRTLGGKGDDLGSYAIQNRQGDYILAGIT
                     SSFGSGSEDVWLVKLALGQEEDSSAKLANLTQSLRSRARPSRSF"
     gene            2999..3715
                     /locus_tag="PLV49_03420"
     CDS             2999..3715
                     /locus_tag="PLV49_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HOG97926.1"
                     /translation="MHLAVRVSDIGLYLLCPRLVYFDSLGKLPRFSSAKQILMRSLAL
                     SLLQKDDLESQLKEALQRLKVELPLVYDLKDDELQAALNEADEEIGSMVNGLKGWMDQ
                     LIPFEAEVELYSEKLGLSGRLDRLAPGDTPSIIRTGRPPENGIWKRDRLMLAGYSLLL
                     GEKENIKVDWGQVEYPSLGMVRRVPVRSTDKARVLRIRDRVRLIKNGQLPDRPEGSPC
                     QSCHAQTMCEMRHSLASKFF"
     gene            3658..4944
                     /locus_tag="PLV49_03425"
     CDS             3658..4944
                     /locus_tag="PLV49_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG97927.1"
                     /translation="MPCTDDVRDEALFGLKVLLIATHPIAFKGGALLLLGKWRITLLF
                     DGWKAHLAVLPTKRKYKMATIEEQIKALEEEIFNTQKNKATEHHLGKIKAKIAKLRAQ
                     QELQKIKGGGGGRRYYIKKSGDATVALVGFPSVGKSSLLNYLTGSKSEVAAYQFTTLE
                     VIPGVMKHKGAEIQILDMPGIIKGAARGKGRGREVITAARAADMILLLGDVFNYNLKV
                     LERELYEAGIRLDKQPPNIHITQEKKGGIIVRSTVALTRMTEFEIAEIIRAYGIVNAN
                     VTVREDIDTDTLVDFLAGNRVYIPSLVAINKFDLRYGGIEDKIEEDLGRDYMPISCAT
                     TEGLEELKDRIYETLGFIRIYLKPKGGKADLEEPLVLLDGSTVKSVCEHLHRDFVNLF
                     RYALVWGKSAKFPGQSIGLDHELQDCDVLSIITKRR"
     gene            5198..5329
                     /locus_tag="PLV49_03430"
     CDS             5198..5329
                     /locus_tag="PLV49_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97928.1"
                     /translation="MTDVTCSECGKQTQVPFKPDGSRPVYCSECYQKHRPAKTSRRY"
BASE COUNT         1344 a         1297 c         1535 g         1215 t
ORIGIN      
        1 ccacctcggt ctcggcaaga tctatcatcc tttgggtgag gtggcgtgag ttcatcgctc
       61 ttaccgcatc tcccaggtta tgggcctcat ctatgatcag ggtgaccttc tccgagtcca
      121 tctccagcca ctggaagatg atgtcctgga agtcaggact gaagaggtga gagtagttca
      181 tgatcactat atcgctgttc ttggcgtaaa ggctcatgat ctcatagggg cagatgccct
      241 ggcaggagtc cataagcccc gagggtgcag tgaccctctt tttcaggccc tctaccacat
      301 ccagggccct ggcagagcgg cggaaatgga cagtaccatt gatctcaaag ccctctctgc
      361 ttcgcaggta gtacgggcag aacgtcctgt aacccggctc ctcctcggga atgctatctg
      421 cataaggatc gtagatggct gcattgccct tgccgatctt cgaggagaca tagtttttcg
      481 tccactctct aagcctcaag cagccggcat agatgctctc tgctcggaac tcgccctcca
      541 gagggcagat cctctgcttt cccaccatat aggcgatctc cggcttgtgg cgggtcttgg
      601 accaaattcg attgatctca tcgatgtaga tatcgatctg agagacggtt ctcaccgcca
      661 ccacgatctt tcccggagcg gcggccagag cggcgctgat gcagctcgtc tttccagtgc
      721 cggtgggagc atctatcatc agaatgccct gctttcctct gctcaccaca tcatagactg
      781 cgtccagcat cctgtcctgg tgaggccgga gagactcata gggcaaataa tccaggtaca
      841 cagaggcatg attccactgg gaacagataa atgctatcca tagaacggaa gaaatagggg
      901 agaaaccatt gcaaaagact ctcttgtata gaaaagttga aattgtattg atgacatcct
      961 tactctgtag cattgatggt gagggtaatg aaagcggaaa ttgcagtctt gatcatgata
     1021 atattgacaa ttgtctttcc tgtattgtct tctccttgcc agaacgagga tggccaagac
     1081 ggcctggctg gatatgagac ctccagattg tcgatcatga gtttagacac gaagaggctg
     1141 gaggagagga tggccggagc cctagttgat atgatgtcca agagccaatc agacggtgag
     1201 ggaaagggtg gcaatggcga ttctatcagc tctggtttga agggaccaaa tccactcaat
     1261 ccggaattgc ctggcacgaa tcattctgta gctggcacat cagtcctcaa ttcttcaatt
     1321 gctaacatat cagcactaaa cttctcaacc actaatgcat caatcaactc atcagctacc
     1381 aatggatctg ccactgatgc attcgctggc aatcaatcta ggctaaactc atcgatcccg
     1441 gccccgggtg gttcaactgg aaatcaaggc ataggttcca gcagcaatgc taagttcgat
     1501 ggctactatg gaatcaagtc ctcccagcat cagatgggcg agaacaacat cgactccagc
     1561 accttcctta gcgggagctt ctctatggaa aaggcagtaa aattccagga tataggaatc
     1621 tagaggtgac caagatacca tgaaaaagac caatatccgc ctggggactg catctcgtcg
     1681 ctttcagtca attctactgg tgttgccact cctggcatcg cttctcttcg tcttcctcat
     1741 ctctttttat catatctctc ccgccgcagc ctctctttat agtggggaac tgacagagga
     1801 atggaacagg acctatggag gaaggtatgg cgatggtgcc tggtcagtcc aggagaccaa
     1861 tgacggcggt tatgtccttg tgggccatac ggcaaccagg ggagagggaa gcgacctgtg
     1921 gctggtaaga gtcgatccag atggaaaccc cctttggagc aggattatgg gtggatcggg
     1981 cgaggacgta ggctattttg tccgggagac cgcagatcgc ggctacatca tcgccggaag
     2041 caccaactcc tttggcttgg gcgaggagcg gctgtggctc atcaggatgg acgggaatgg
     2101 aagcctgctc tgggataaga cctttggcgg ttttgtgcac tcttcaggcg atggtggctg
     2161 gtcagtggat gaaaccgatg atgggggata tatcgcaacc ggctataccc aatcaaagag
     2221 cattggtaga aaggacctct ggctcatccg taccgacagc aacgggaatc tgctatggga
     2281 tagaagctat ggaggggcag atgaggatgt ggggttgtct gtactgcaaa gccaggatgg
     2341 cggctttatt gtggccggca ggacggcatc cctggggaaa aggggggacg atatctggct
     2401 cttgaagacc gatgaccagg gcgtcatgag ctggaattct agcttcggcg gagataagga
     2461 tgatgtcgcc ttccaggtgg cggagctggc caatggttac gccctggtgg gcaggtcgga
     2521 gtcaggcctg gaggaggaga agatcattct tatccggctg gatcagctag gccggaagat
     2581 ctgggagaga tcatacctgg gaagctcggg aacatccctg cagcagacga tggatggcgg
     2641 ctttgtcatc ggtggaagga tcgataatgc tgattcggga agggatgctc tgatcataaa
     2701 gaccaactct gccggactgg aggagtggag aaggactctc ggcggtaaag gggatgatct
     2761 cggaagctat gccattcaga atcggcaggg agactatatc ctggctggaa taacctcatc
     2821 ctttggatcg ggatctgaag atgtctggct ggttaaattg gccttggggc aggaggagga
     2881 cagttctgcc aagctggcta acctgactca atcgctgcga tccagggcca ggccatcgcg
     2941 ctctttttag gaggtaatat ccaaaaatct tataaagatc cgggcagata gggagattat
     3001 gcacctagcc gtcagggttt ccgatatcgg tttgtacttg ctgtgcccaa ggcttgtcta
     3061 tttcgattct ctgggaaagc tgccgagatt cagcagtgcc aagcagatcc tgatgcgcag
     3121 cctcgctctc tcattgctgc agaaggatga tctggaaagc cagcttaagg aagctctcca
     3181 gaggctgaag gtggagctgc ctttagtcta tgatcttaag gatgatgaac tgcaggccgc
     3241 cttgaatgag gcggatgagg agattggatc gatggtcaac ggtcttaaag gctggatgga
     3301 ccagctcatc ccctttgagg cagaggtaga gctctattcg gagaagctgg gtctctccgg
     3361 caggctggat aggctggctc caggagatac gccctcgatc atccgcaccg gccggccgcc
     3421 agagaacgga atctggaaga gggaccgtct catgctggcc ggctattcac tccttttagg
     3481 agagaaggag aatataaagg tggattgggg acaggtggag tatccaagcc tgggcatggt
     3541 gcgaagggtg cccgtccgca gcacagacaa agcccgggtg ctgcgcattc gtgatcgagt
     3601 tcgccttatc aaaaacggcc agcttcccga ccggccggaa ggctccccct gccagtcatg
     3661 ccatgcacag acgatgtgcg agatgaggca ctctttggcc tcaaagttct tctaattgcc
     3721 acccatccca tagcttttaa aggaggagcc ctattgctct tgggaaagtg gcgcatcaca
     3781 cttctatttg atggctggaa ggctcatttg gctgtcctgc ccaccaagag gaaatacaaa
     3841 atggcaacca tcgaggagca gatcaaggcg ctggaagagg agatcttcaa cactcagaag
     3901 aacaaggcca ccgaacatca tctgggcaag attaaggcca aaatagcgaa gctgagggcc
     3961 cagcaggagc ttcagaagat caagggcggc ggagggggca gaagatacta tatcaaaaaa
     4021 tcaggcgatg ccacggtggc cctggtggga ttcccctcag tgggcaagtc cagcctgctc
     4081 aattatctga ccggatccaa gtccgaggtg gctgcctatc agttcaccac cctcgaggtc
     4141 attcctgggg tgatgaagca caaaggcgcc gagatccaga tcctggatat gccagggatc
     4201 atcaaaggag cggcgcgggg aaagggcagg ggcagagagg tgattactgc cgcccgggcc
     4261 gcggatatga tcctcttatt gggggacgta ttcaattaca atctgaaggt cctggagcgg
     4321 gagctctatg aggccggcat aaggctggac aagcagccgc caaacatcca catcacccag
     4381 gagaaaaagg ggggcatcat agtgcgcagc actgttgccc tgacccggat gacggagttc
     4441 gagatcgcag agatcatccg cgcctacggc attgtgaacg ccaatgtaac cgttcgggag
     4501 gatatagaca ctgacaccct ggtcgacttc ctggccggga acagggtcta tattccctct
     4561 ctggtggcca taaacaagtt cgacctgcgc tacggtggga tcgaggacaa gatcgaggaa
     4621 gacctgggca gggactatat gcccatatcc tgcgccacca ctgaaggcct cgaggagcta
     4681 aaagaccgta tatatgagac gctgggattc attcgcatct atctcaagcc caagggaggc
     4741 aaagcagatc ttgaggagcc tctggtgctc ctggatggaa gcacagttaa atcggtatgc
     4801 gagcatcttc atagggactt cgtaaaccta tttcgatatg cactggtctg gggaaagagc
     4861 gccaagttcc ctggccagtc catagggctg gatcatgagc tccaggattg cgatgtgctc
     4921 tcgataatca ccaagaggag atgatcggcc ggagttggcc gggcattttg gcagcgaaaa
     4981 ggcggctgct atcatatttt tttaccaaca agtatatatg ctcagccatc ctccttatac
     5041 tgctgcattt cgtctatcaa agtaatttgt tgagatgttt tctgtagcag tctcgattct
     5101 tttgagtcga ttcaaaaggt aagtgaatta aatgtttgag agaaaatatg aaaatagtgg
     5161 tagcagcaga ggcagctaca atagtggtcc gagagaaatg actgacgtaa cctgctcaga
     5221 atgcggcaag cagacccagg ttccatttaa gccggatgga tccagaccgg tctactgcag
     5281 cgagtgctat cagaagcaca gacctgccaa aacatccaga agatattaag ctgatttagc
     5341 atcacataat agtcgatccg ttttgatttg aaggcaggtg ctttcgggca a
//
LOCUS       DAOSGP010000076         5339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13658_length_5339_cov_17.189061, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000076 DAOSGP010000000
VERSION     DAOSGP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13658_length_5339_cov_17.189061"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1693
                     /locus_tag="PLV49_03435"
     CDS             23..1693
                     /locus_tag="PLV49_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOG97929.1"
                     /translation="MEDFSILIGGIAGDGINEAGLTAARLMNRLGYRIFMYYDYPSLI
                     RGGHNFSMVRASSERIGVCRDEIDLLIALNQDTVDRHSGRLKESSIFIFDADNVMGDG
                     IAEKSCGISVTGILKEEGAPSVMKNSCILGAFCHAVGIEWTVLEDVLKKHIPKKLELN
                     LKVARRGYDQATEFCRIADLDNGPEPIITGNQAISLGLIRAGLEAYVAYPMTPSSAIL
                     DFMANSAEEFGLKVVHPESEIAVILMAEGFAYAGVRVAVGTSGGGFCLMTEALSLAGM
                     AEIPLAIVMAQRTGPSTGLPTYTAQGDLHFVINAGQGEFPRLVVAPGDAEDAYLWSAA
                     ALNLAWKYQIPAFILSDKIVSESQYSFDLGLAGKAETARPALLWNGQGDYHRYRHSDT
                     GVSPLAYPPQRRQVIKADSYAHDATGITTEDAQITREMSDKRMLKAQSLAGELEGYET
                     VKLLGDERSRTCLLFWGSNKGVCREAAESLGLRAVQVLVLWPFPEKRLKDALRGVERL
                     IAVECNATGQLATLCRQYGIEVDDRILKYGGRPFSLNDLETELERREA"
     gene            1690..2541
                     /locus_tag="PLV49_03440"
     CDS             1690..2541
                     /locus_tag="PLV49_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG97930.1"
                     /translation="MKPQDLSTAARNTWCPGCGNFSILNAIRAVLARMDEEGFPVENT
                     VLVSGIGCHGKIVDYLDVNSFYSIHGRTIPVATGIKLANPDLNVICFSGDGDSLAEGL
                     DHLIFAAKRNIDITLILHDNRVYGLTTGQYTPTSPTGFRGKSTPEGSIERPLNPLDLM
                     LSAGATFIARGYSHGIEQLQTLFKEAILHKGFSFVDVLQVCVSYFNMYESYNRRTYSV
                     EGNDAASFDQAQEIIRRWSYDDSESPIPLGKFYQIDAPRFDQAYSQTKPLLARGDKIG
                     SKLESFI"
     gene            2578..2739
                     /locus_tag="PLV49_03445"
     CDS             2578..2739
                     /locus_tag="PLV49_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOG97931.1"
                     /translation="MDKYVCTVCGYIYDPEKGDSASGIAPGTAFEDLPDDWTCPECGV
                     GKELFNKVE"
     gene            2779..3303
                     /locus_tag="PLV49_03450"
     CDS             2779..3303
                     /locus_tag="PLV49_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOG97932.1"
                     /translation="MLSERMNEALNYQANRELYSSYLYLSMSYYFESIGFRGFASWMR
                     VQAGEELVHTMKLLDYVAASGEKAKMLAVEAPQHSWESPQQAFAHVWEHERAVTRMIH
                     DLVAVAESESDAMTRKFLQWYVDEQVEEEESSDGVLKRVQAAGSDPKALRAADEELGK
                     RGFKFPRGFQMFPL"
     gene            complement(3363..>3736)
                     /locus_tag="PLV49_03455"
     CDS             complement(3363..>3736)
                     /locus_tag="PLV49_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97933.1"
                     /translation="AEDENFSVNISASKFLGSYLVNETGFALYYYQNDSSLDGMSTCN
                     DDCAKMWIPFYVEDLTLPESLNPSNFATIERADGSKQTTFKSWPLYLYSRDSSPGDAR
                     GNGLEDDQWRVIDPANQPELI"
     assembly_gap    3737..3836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3837..4690
                     /gene="tgt"
                     /locus_tag="PLV49_03460"
     CDS             <3837..4690
                     /gene="tgt"
                     /locus_tag="PLV49_03460"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG97934.1"
                     /translation="RADGKGQKNAKKENLTKITDQGISFKSMADGAWHFLDAERSMRI
                     QANLGSDIIMAFDECTSPLSDYDYTRKAMCRTHDWAEESIRYHDKSQAIYGIIQGGWF
                     EDLRRTSTDFIVSQPFDGIAIGGSLGNSKQDMHQVLDWTIPRLDDRPRHLLGIGEIDD
                     IFECVARGIDTFDCVSPTRIARRGSLLLSPQSGGSRQNKFRLNIKSARFKEDDLPVDP
                     ACSCPTCCRYSRAYLRHLYVSGELSYFRLATLHNLHFMLRLMESIRESIRCGSFMELR
                     DRWLKAI"
     gene            4672..>5339
                     /locus_tag="PLV49_03465"
     CDS             4672..>5339
                     /locus_tag="PLV49_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HOG97935.1"
                     /translation="MAKSDLAEKGSSVAWGRLKLDKTQATIILCGFYLFFSLVGNIAA
                     TKVTYFGSLVMDAGFIYSLTFTWRDLIHKQLGERAALTTIWLAASFNLLAAIYFQFVV
                     LLPAQTDWANSGGQAAWTFLFGILDSSGQGWQSVLSLQMRIVLASILTALLAELIDTR
                     VYRIWTRGARKSWPQWTRVFASNAVSIPIDSLLFPVIAFMGILGTQGLVQMIWTNMIV
                     KAAIT"
BASE COUNT         1189 a         1420 c         1513 g         1117 t
ORIGIN      
        1 ttgaattgca cagggtgatt tgatggaaga cttctctatc ctcataggcg gcatcgccgg
       61 tgacggcata aacgaggcgg gtctgactgc cgcccggctc atgaaccgcc tgggctatcg
      121 catattcatg tactacgact atccctctct gatcagaggc gggcacaact tctcgatggt
      181 ccgagcctca tccgagagga tcggcgtctg cagggatgag atagacctgc tcatcgccct
      241 caatcaggat acagtagata gacattccgg ccgcctcaag gagagctcaa ttttcatttt
      301 cgatgcagat aacgtaatgg gagatggcat agccgagaag agctgcggca tttccgtcac
      361 cggtatcctg aaagaggagg gggccccttc cgtcatgaag aacagctgca ttctgggtgc
      421 cttttgtcat gcggtaggca tcgaatggac ggtgctggaa gatgtcttga aaaagcatat
      481 cccaaagaag ctggagctga acctcaaggt cgcccgccgg ggatatgacc aggccacgga
      541 gttctgccgc atcgcagatc tggacaacgg tcctgagccc atcataaccg gcaaccaggc
      601 catcagcctg ggcctgatca gggcaggtct tgaggcctat gtggcctatc ccatgacccc
      661 atcctcggcc attcttgact tcatggccaa ttcggcggag gagttcggcc tgaaggtcgt
      721 ccatccggag agcgagatcg ctgtgatcct catggcagag ggattcgcct atgccggtgt
      781 ccgggtagca gtggggacct cgggaggagg attctgtcta atgaccgagg ccctcagcct
      841 ggcagggatg gctgagatcc ccctggccat cgtcatggca cagaggactg gaccctcaac
      901 cggcctgccc acctataccg ctcagggcga cctgcatttt gtgatcaatg ccggccaggg
      961 ggagtttccc aggttggtgg tcgccccggg tgacgcagag gatgcatatc tctggtctgc
     1021 tgcagccctg aacctggcct ggaaatatca gattcccgcg ttcatcctct cggataagat
     1081 cgtaagcgag agccagtata gctttgacct cgggctcgca ggaaaggcag agactgctcg
     1141 accggcactg ctctggaatg ggcagggtga ctaccatcgc taccgccact ccgatacggg
     1201 ggtctctcca ctcgcctatc ctcctcagag gaggcaggtg ataaaggcgg acagctatgc
     1261 gcacgatgcc acaggcataa ccactgagga cgcccagata acaagggaga tgagcgacaa
     1321 aagaatgctc aaggctcaat ccctggccgg ggaattggag ggatacgaga ctgtgaagct
     1381 cttaggtgat gagaggagca ggacctgcct cttgttctgg ggctcgaaca agggggtatg
     1441 cagagaggcc gctgagagtc tgggactgag ggcggttcag gtgctggttc tctggccgtt
     1501 ccctgagaag aggctgaagg atgccctccg gggtgtggag aggctgatcg cagtggaatg
     1561 caatgccacc ggccagctgg cgactctatg ccgccagtat ggcatcgagg tcgatgaccg
     1621 aatcctgaaa tacggcggcc ggcctttttc cctgaacgat cttgaaaccg agctggaaag
     1681 gagggaagca tgaagcccca ggatctatcc accgctgccc gcaatacctg gtgtcctggc
     1741 tgcgggaact tctccattct gaatgcaata agagcggtcc tcgcccggat ggacgaggag
     1801 ggattcccag tagagaacac cgtcctggtt tcgggcatcg gctgccacgg caagatcgtg
     1861 gactacctgg atgtgaacag cttctactcc atccacggcc ggaccattcc cgttgccaca
     1921 ggcataaagc tggccaaccc cgatctgaat gtgatctgct tctctggaga cggcgacagc
     1981 ctggctgagg gtctggacca cctgatcttc gccgccaaga ggaacatcga cataaccctg
     2041 atcttacatg acaaccgggt ctacggcctg accaccggcc agtacacgcc cacctcacca
     2101 accggattcc ggggcaagtc cactcctgag ggatccattg agcgacccct gaaccctctg
     2161 gaccttatgc tctctgccgg cgccaccttc atcgcccggg gctactctca cgggattgag
     2221 cagcttcaga ccctctttaa agaggcgata ctgcacaaag gcttctcatt tgtggatgtc
     2281 ttacaggtct gcgtctctta cttcaatatg tatgagagct ataacaggag aacctattcc
     2341 gtggaaggaa acgatgccgc cagctttgac caggcccagg agatcatacg ccgctggagc
     2401 tatgacgact ccgagtcgcc catacccctg ggcaagttct atcagattga tgcaccgcga
     2461 ttcgatcagg cctacagcca gacgaagccg ctcttggcga gaggggataa gatcggatcc
     2521 aagctggaaa gtttcatcta gcaagaaggc aaaaaacgaa ggagtggtga gacagaaatg
     2581 gacaaatacg tatgcactgt ctgtggatat atctacgatc cagagaaagg ggattcggca
     2641 tccggcatag caccagggac ggctttcgag gatctgccgg atgactggac ctgtccggag
     2701 tgcggcgtgg gaaaggaatt gttcaataag gtggagtaaa gctcagaatc tgccgtttta
     2761 tctgctcagg agatcactat gctcagcgaa aggatgaatg aagccctcaa ttatcaggca
     2821 aatcgggagt tatactcctc ctatctgtat ctttccatgt cctactactt tgaatccatc
     2881 ggattcaggg ggtttgccag ctggatgagg gttcaggcag gagaagagct ggtccacacc
     2941 atgaaattgc tggactatgt tgccgcttcc ggcgagaagg cgaagatgct cgccgtggag
     3001 gcgccacagc atagctggga gtcgccccag caggcatttg ctcacgtctg ggagcacgag
     3061 cgggcggtga ccaggatgat tcatgatctg gtggcagtgg ccgagagcga atcggacgca
     3121 atgacacgga aattcttgca gtggtatgtg gacgagcagg tggaagagga ggagtcctcg
     3181 gacggtgttc tgaagagggt gcaggctgcc ggaagcgatc caaaagccct ccgggcagcg
     3241 gatgaggagc tgggcaagag aggcttcaaa ttcccccgcg ggttccagat gtttccattg
     3301 taaaaggtgt gaggccacta tttggggcct caaatcgttt ttaatcacca attttttgct
     3361 cttcagatga gctcgggctg attggccgga tcgatgacgc gccactgatc atcctccagg
     3421 ccattcccgc gagcatcgcc tggtgaactg tcccgggaat agaggtacaa tggccagctc
     3481 ttgaatgttg tctgcttaga accatcagct cgctcgatgg tggcgaagtt ggatggattg
     3541 aggctctcag gcagggtcag atcctcgaca taaaagggga tccacatctt tgcacaatca
     3601 tcattacagg tgctcattcc atcgagactg ctatcattct ggtagtagta aagggcaaat
     3661 cccgtctcgt tcaccagata gcttcccagg aacttgctcg cggagatgtt cacactgaag
     3721 ttctcatcct ctgccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaag
     3841 ggcggacggg aaggggcaga agaatgccaa gaaggagaac ctgacaaaga tcacagacca
     3901 gggtatctct ttcaagtcca tggccgatgg ggcctggcat ttcctggatg ctgaaaggtc
     3961 gatgaggatc caggcaaacc taggctcgga tatcatcatg gcctttgacg agtgcacctc
     4021 ccccctgtca gactacgatt ataccagaaa ggccatgtgc aggactcatg actgggcaga
     4081 agagtccatc cgctatcatg ataagagcca ggccatctac ggaatcatcc agggagggtg
     4141 gtttgaggac ctgaggcgga cgagcaccga ctttatcgtc tcccagccct ttgacggcat
     4201 agccatcggc ggatctttgg ggaacagcaa gcaggatatg catcaggtct tggactggac
     4261 gatccccagg ctggacgacc ggccgcggca cctattgggg atcggggaga tagatgacat
     4321 cttcgagtgc gtggccaggg ggatcgatac cttcgactgc gtctctccca ccaggatcgc
     4381 ccgcagagga agcctcctcc tatcgcccca gtccggtgga tcgcggcaaa acaagttccg
     4441 cctgaatatc aagtccgccc ggttcaagga ggacgatctg cccgttgatc ctgcctgctc
     4501 ttgccccacc tgctgcagat attcccgagc ttatctccgc catttgtacg tatcaggaga
     4561 gctctcctac ttccgtctgg ccaccctgca taacctccat ttcatgcttc ggctgatgga
     4621 gagcataagg gaaagcataa gatgtggcag cttcatggag ctcagggaca gatggctaaa
     4681 agcgatttag ctgagaaagg atcttcggtc gcatggggac ggctgaagct cgataagact
     4741 caggccacca taatcctctg cggcttttac ctcttctttt ctctagtggg aaacatcgcc
     4801 gccaccaagg tcacatactt cggcagcctg gtcatggatg ccggtttcat ctattctctg
     4861 accttcacct ggcgggacct gatccacaag cagctggggg agagggcggc cttgaccact
     4921 atctggctgg cagcgagctt taatctgctt gcggccatct attttcagtt cgtggtcctc
     4981 ctgcccgccc agactgactg ggcaaatagc ggtgggcagg cggcctggac atttctcttc
     5041 gggatcctgg acagctcagg ccaggggtgg cagtcggtcc tctccctgca gatgagaatc
     5101 gtcttagcct ccattctcac ggccctcctg gcggagctga tcgacaccag ggtctatcgc
     5161 atctggaccc gtggagcgcg caaaagctgg ccccaatgga cgcgagtctt cgcctccaat
     5221 gccgtctcca tacccataga cagccttctc tttccggtga tcgcctttat gggtatcttg
     5281 ggaactcaag ggctggtgca gatgatctgg accaatatga tcgtcaaagc agcgatcac
//
LOCUS       DAOSGP010000077         5293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13829_length_5293_cov_17.789423, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000077 DAOSGP010000000
VERSION     DAOSGP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13829_length_5293_cov_17.789423"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3244)
                     /gene="smc"
                     /locus_tag="PLV49_03470"
     CDS             complement(<1..3244)
                     /gene="smc"
                     /locus_tag="PLV49_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOG97936.1"
                     /translation="MYIKEVELKNFKSFGKSIRVPLKNDFVTVTGPNGSGKSNIVDAL
                     LFALCLSSSRAMRAERLPDLIYRGDNGKNPDFAQVIVRLDNTSRHFPLDQDTIEVSRK
                     IKINRDKYASSYSFNGKSCGQAELLDLLAKAGITPESYNIVMQGDVTRIIEMTAVERR
                     KIIDEIAGVAEFDEKKKKALEELDVVRERVERVDVILEEVGGQLDRLRAERDKAHSYQ
                     AHKDELKRQEAFLLLARLKEASGELESLNQEISSFQGKNDEIKQRADEDRSELAVQEE
                     KLQNLGQEITHKGEDEQIEVKRRIEELKGELARETSKIQMAEKSLNDLDRQQNASFIQ
                     LGNLSGEEEVLAEKIRDASIRKASLEGELEDQNDLLQEVEESLTLEDAQFSALREELE
                     RARGIREEAKARLADLLRERDRLLDGTRRGSLEREELSGAIKEAMDALAGSDHEADQL
                     KEELAGLNSRAMELEKDRDDLESARLRLRREIAEAEREMNKLQSEFARTDSRIKAAED
                     KGGFSRAVDAIRTALKKDMLPGLYGTVAELGNVQSRFSVALEVAAGARMQSIVAATDE
                     DAARAIEYLKRSQIGRATFLPLNKLDRGSPSVKPNYEGIVDYAFRLIDFDPKFQGAFW
                     YVFRDTLVVESLNHARALMGRYRMVTLEGDLVERSGAMTGGHYRTRMKFAAEEGKKLL
                     ELSERIANADAERGKLLERLDRMEEQISHITREVEELNRSISKKTFQADEMAGSKPRL
                     EKSISEKRERLVQMESEFLSYKGRLDVLEGEIGESEATLASMNEMIGRIEADLSGSAI
                     PELNRKADAAKSEIRRLQDRIAEIDAEILKDKIREEGIIEKQKEISSKKELLESEKAE
                     AAEEKEAAMAMIQSLNLELDGMKSREAEIEVELHGLKGVRVELLEKTITMQRAIDRME
                     REMDRIQARLDASLSARDVIQMRVEGLRREIEQSGVDSTQEPPDSNTVAEKIRALTQF
                     MRDLEPVNMLAIDEYDHVLTRHDFLALRRTTLREEREAIIDKLERYDQMKKEAFLSSF
                     NEINKNFKNIFQELSRGEGDLILENPEDPLSGGMTIKARPAGKPFHRLEAM"
     assembly_gap    3448..3547
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3548..4377
                     /gene="mmp10"
                     /locus_tag="PLV49_03475"
     CDS             <3548..4377
                     /gene="mmp10"
                     /locus_tag="PLV49_03475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719926.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HOG97937.1"
                     /translation="VNEVSFTVFSTNPELRRKYMGDKHPQVALENLKTFCHSCEVYCA
                     AVIIPGVNDGQELEKTCNDLEEMGARGLILMRFANRRDQGLILGNDPIMPGIIPHTIE
                     EFRDLVTHTAEQHRFRVTGTPLWDPVTGAPFALSRHPDIISRLPPLRRGASIITSRVA
                     APLLKGIFSQMEGGEKVSILPVEKDIGCLITIEDFSGLDLDQVMGTVIIPGRTMAHEK
                     EIKSVLSKDGRERMIIRGPDTLTVDGEMSISMNAQDVIELEVEAFVELIEAINALGI"
     gene            4416..>5293
                     /locus_tag="PLV49_03480"
     CDS             4416..>5293
                     /locus_tag="PLV49_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04083 family peptide-modifying radical SAM
                     enzyme"
                     /protein_id="HOG97938.1"
                     /translation="MKNVRRQKRPFHVMIIPTLGCPSKCSYCWSSEVGSPRMTIETVE
                     EIVEWLRDFRDDPVTFTFHGGEPLLAGADYYRAALPMLKRELEHLSPNFALQTNLWLL
                     TPELAEVLAEHEIPIGSSLDGPQELNDLQRSRGYYERTIAGYDLAKKHGLQVQFICTF
                     TSYSVQFKEEIFNFFLENGLVLKLHPALPSLRSNEPDRWALPPEEYGELLVYLLDKYL
                     ENMDRIEVRNINDYARCVFTGRGTVCTFVDCMENTFAVGPDGSIYPCYRFVGMPEFVM
                     GNVRDRPSQADLAASPA"
BASE COUNT         1022 a         1613 c         1292 g         1266 t
ORIGIN      
        1 acatggcctc caggcggtgg aagggctttc ccgccggccg ggccttgatg gtcatccccc
       61 cgctcaaggg atcctccggg ttctccagga tcaggtcgcc ttctccccgg gatagctcct
      121 ggaatatgtt cttgaagttc ttgttgatct cattgaagct ggataagaat gcctcctttt
      181 tcatctggtc atacctctcc agcttatcga tgatcgcctc cctctcctcc cttagggtgg
      241 tcctcctcaa ggccaggaag tcatggcggg tcaacacatg atcgtactcg tcgatggcca
      301 gcatgttcac cggctccagg tctctcatga attgggtcag ggcccggatc ttctcagcga
      361 ctgtattgct atccggcggc tcctgggtcg agtctacgcc gctctgttcg atctctcttc
      421 gcagtccctc caccctcatc tggatgacat ccctggcgga gaggctggca tccagacgag
      481 cttgaatccg gtccatctcc cgttccatcc tgtcgatcgc ccgctgcatg gtgatggtct
      541 tctcaagaag ctctacgcgc actcctttga ggccgtgcag ctccacttcg atctccgcct
      601 ccctggactt catcccatcc agttccaaat tcaggctctg gatcatggcc atggcagcct
      661 ccttctcctc tgctgcctct gccttctccg attccagtag ctcctttttg gatgagatct
      721 ccttttgctt ctcgattatg ccctcttccc ggatcttgtc cttcaggatc tcggcatcga
      781 tctcagctat ccggtcctgc agccttctaa tctccgattt ggccgcgtct gccttgcgat
      841 tcagctcggg gatcgctgag cctgataggt cggcctctat ccggccgatc atctcattca
      901 tggatgccag ggtcgcctca gactccccga tctccccctc cagaacgtcc agccttccct
      961 tgtagctgag aaactcgctc tccatctgca cgagccgctc gcgcttctcg gagatgctct
     1021 tctccagccg gggctttgac cccgccatct cgtccgcctg gaaggtcttc ttggagatgg
     1081 accggttcag ctcctcaacc tctcgggtta tgtgggagat ctgctcctcc atccggtcca
     1141 atctctccag aagtttcccg cgctcggcat cggcgttggc aatcctctcc gatagctcca
     1201 ggagcttctt tccctcctct gccgcgaact tcatcctggt cctatagtgg cctccggtca
     1261 tcgctccgga cctctccacc agatcccctt ctaaagtgac catccggtag cggcccatga
     1321 gagctcgggc gtggtttaag ctctctacca ctaaagtatc gcgaaataca taccagaaag
     1381 ccccctgaaa cttgggatca aagtcgatca gcctgaaggc atagtcaact atcccctcgt
     1441 agttgggctt gaccgaaggg ctgccccggt ccagcttatt caaggggaga aacgtggccc
     1501 tgccgatctg ggacctcttc agatattcaa tggcccgggc ggcatcctcg tccgtggcgg
     1561 cgacaatact ctgcatccgc gctcctgcag ccacctctaa agcgacagaa aagcgcgact
     1621 gaacattccc cagttccgct acggtgccat aaaggccggg gagcatatct ttcttgagag
     1681 cagtacggat ggcgtccacc gcccggctga atccgccttt gtcctctgcc gccttaatcc
     1741 ggctgtcggt gcgggcgaac tcgctctgca gcttattcat ctccctctct gcctcggcga
     1801 tctcccggcg cagcctgagt cttgcgctct ccaggtcatc ccgatccttc tccagctcca
     1861 tcgctcggct gttgagcccg gccagctcct ctttcagctg atcggcctca tgatcgctgc
     1921 cggccagggc atccatggcc tccttgatgg cgccggagag ctcctctctt tccaggctcc
     1981 cccttctcgt gccatccagt aggcggtccc gctctcgcag cagatctgct agccttgctt
     2041 tggcctcctc tcggatcccc ctcgccctct caagctcctc tctgagggcg gagaactggg
     2101 cgtcctcaag ggttaagctc tcttcaacct cctggaggag gtcgttttga tcttcaagct
     2161 ctccttctaa gctggccttg cgaatgcttg cgtctctgat cttctccgcc agaacctcct
     2221 cctccccaga gaggttaccc agctgaatga agctcgcatt ctgctgacga tccagatcgt
     2281 tcagggactt ctccgccatc tggatcttgc tggtctcgcg ggcgagttcc cctttcagct
     2341 cctctatcct ccgcttgacc tcgatctgct cgtcctctcc cttatgggtt atctcctgac
     2401 cgaggttctg cagcttctcc tcctggacgg caagctcgga gcgatcctca tctgcccgct
     2461 gcttgatctc atcattcttg ccctggaagg aggatatctc ctggttcagg ctctccagct
     2521 ctccagatgc ctctttgagc ctggccaaga ggaggaaggc ctcctgcctc ttcaactcgt
     2581 ccttgtgcgc ctgataggaa tgggccttat cccgctccgc ccgcaatcgg tccagctggc
     2641 cgcccacctc ctccaggatg acgtcaaccc gttcgacgcg ttctcgcacc acatccagct
     2701 cttccagcgc cttcttcttc ttctcatcga actctgcgac gccagcgatc tcgtctatga
     2761 tcttcctccg ctccacagcc gtcatctcaa tgatgcgggt cacatcccct tgcatgacga
     2821 tattgtagct ttccggggtt atgcccgcct tggccagcag gtccaaaagc tctgcctggc
     2881 cgcaggactt gccattaaag gaataagaag aggcatattt gtcgcggttt atcttgatct
     2941 ttcttgagac ctcgatcgta tcctgatcca gtggaaaatg gcggctggtg ttgtccagcc
     3001 tgactattac ctgggcaaaa tccggattct ttccgttatc cccccggtag atgagatcgg
     3061 gcagcctttc cgccctcatg gcccgcgagc tggaaaggca gagggcgaag aggagagcat
     3121 cgactatatt ggatttaccg cttccgttcg gccctgtcac cgttacaaaa tcgttcttca
     3181 gaggaaccct gatgctcttg ccgaatgact tgaagttctt gagctcaacc tctttgatgt
     3241 acaaaggtta cctccgccca aattctgcaa tgctggccct ttcgatgctc ctcaacatta
     3301 gaatcatgtc cctcggcttt aagcccgctc ttttttctta aggcagttga ggctttcgct
     3361 tatgattgct gctctaactt aatcatgtat tcgaggatgt atatatcact ttcccatatt
     3421 taagcatttt atccagtact attattannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnngag tgaacgaggt ctctttcacc gtcttctcca ccaaccccga gcttcgacgc
     3601 aaatacatgg gagacaaaca tccccaggtc gccctggaaa atttgaagac attctgccac
     3661 agctgcgagg tctactgcgc cgcagtcatc attccgggcg taaacgatgg ccaggaattg
     3721 gagaagacct gcaacgacct agaggagatg ggagcaagag gcctgatcct gatgaggttt
     3781 gccaatcgcc gggatcaagg cctgatcctg ggaaatgatc ccattatgcc agggataatc
     3841 cctcatacca tcgaggagtt cagagacctg gtcacccata ctgcagagca gcaccgattt
     3901 cgggtaactg gaacgcctct atgggatccg gtcaccggcg cgcccttcgc tctgtccagg
     3961 catccagata tcatatcccg cctgccccct ctgaggagag gggcgagcat catcaccagc
     4021 cgggtggctg ctcctttgct caagggcatc ttcagtcaga tggagggagg agagaaagta
     4081 agcatcctgc cggtggaaaa ggacatcggc tgcctgatta ccattgagga cttctcaggc
     4141 cttgatcttg accaggtcat gggcacggtc atcattcccg gccgaaccat ggcccatgag
     4201 aaagagatca agagcgtcct gtcaaaagac ggcagggaaa ggatgataat tcggggcccg
     4261 gatactctca ccgtggacgg cgagatgagc atctccatga acgcccagga cgtcatagag
     4321 ctggaggtcg aggcattcgt agagctgatc gaagccatca atgccctggg catatgaata
     4381 taggcatatg aagaaagcaa agggggcaaa aatagatgaa aaatgtgaga agacaaaaga
     4441 ggccgtttca tgtcatgatc atacccaccc tcggctgtcc ctccaagtgc agctactgct
     4501 ggagctccga ggtcggatcc ccgagaatga ccatcgagac agtagaggag atcgtcgaat
     4561 ggttgcgcga ttttagggat gatccggtta cattcacgtt tcacggtgga gagccgctgc
     4621 ttgccggtgc ggattactac agagctgccc ttcccatgct caaaagagag cttgagcatc
     4681 tcagccccaa cttcgccctg cagacgaacc tgtggctctt gaccccagag ctggcggagg
     4741 tcctggcaga gcatgagatt cccataggct caagccttga cggccctcag gagctcaatg
     4801 acctgcaaag gtctcgtggc tactacgaga ggaccattgc aggatatgat ctagccaaaa
     4861 aacatggcct gcaggtgcaa ttcatctgca cattcacatc atattcagtg cagttcaaag
     4921 aggagatatt caacttcttt ctggaaaacg gcctggtcct caagctccac ccagcactcc
     4981 catcgctgcg cagcaatgag ccggaccgat gggctcttcc cccagaggag tacggcgagc
     5041 ttttggtcta tctcctggac aaatacctgg agaacatgga ccgaatcgag gtgaggaaca
     5101 tcaacgatta cgcgaggtgc gtattcaccg gaaggggtac cgtctgcaca ttcgtggact
     5161 gcatggagaa tacctttgcc gtgggcccag atgggagcat ctatccatgc tatcggtttg
     5221 tgggaatgcc cgaattcgtc atgggaaacg tccgcgaccg ccccagccag gcggatctgg
     5281 ccgcctcccc cgc
//
LOCUS       DAOSGP010000078         5252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_13979_length_5252_cov_16.035790, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000078 DAOSGP010000000
VERSION     DAOSGP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13979_length_5252_cov_16.035790"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..677)
                     /locus_tag="PLV49_03485"
     CDS             complement(15..677)
                     /locus_tag="PLV49_03485"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HOG97939.1"
                     /translation="MNRPFVFINSAMSADGKISSRDRRQIRISGKEDLMRVDELRAAS
                     DAIMVGVGTVLADDPGLRVKSRILQEKRTAMGQSKEPFRIVADSNARTPANAKILGPG
                     CMIAVSYSAPEERLDRLRPGCEIIKCGTEMVDLPGLMEELHNRGVRRLMVEGGARLNW
                     SLIELGLVDEIYIYMGGLIIGGERAPSLVDGPGFRNSFPRLSLQSVQRLDEGLLLQWK
                     LH"
     gene            775..1875
                     /locus_tag="PLV49_03490"
     CDS             775..1875
                     /locus_tag="PLV49_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG97940.1"
                     /translation="MSNIAKPKVAPPGAARLKQDELILQGTIIYGDDFQPIEGYISIR
                     DGIIKEAGTGQVDADFEGIVCPRFVNSHIHLGDSAFKDPPFLPLSELVGPKGLKISLL
                     CETPRERLIEGMRRSLAQMVDSGTYAFADFREGGEAGVRMLQEAAKDMPLLVRVFGRP
                     AQGKTETPEGCTGLGISSTRDFDPSLIQRMVLEARRASKAVAIHAGESARDDIPDAFS
                     LLPDHLVHMSRAEESDLRSVAQKGTPLVICPRSNLITGSGLPNVKRMIELGITVGVGT
                     DNVMLNSPNMFMEMHLISRALLHDDRQVFKMCTLNGAEIMGLDQKLGSIREGKEAKVM
                     VINGSSDNLWGSTHPLRSIVRRAEPTDILAIF"
     gene            1897..2334
                     /locus_tag="PLV49_03495"
     CDS             1897..2334
                     /locus_tag="PLV49_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG97941.1"
                     /translation="MFEKILAATDGSEHGLRAAKVAVELGKISGGKVFAVYVADTQRT
                     AHLPDDMLLFSIKELLLKEGKEAIKQVQELAEKNGVDFDGAVVEGNPSDEIISYAKKK
                     GANVIIVGAVGRTGLDKFLLGSVAEKVVRNSRIPVLTVPREES"
     gene            2331..>3149
                     /locus_tag="PLV49_03500"
     CDS             2331..>3149
                     /locus_tag="PLV49_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG97942.1"
                     /translation="MKILVRDVMVREVAYAEIPGNRDDVLKALQDRKVSGLPVVKKGE
                     VVGIITRSDLLRNREEDQTALLMTRDPVVISPDRSIVEASKLLIQHKIRRLPVVEGKE
                     LVGLVTVADIVRVAEGMNIEESIEPYLEKETVVLWSEMPLPVAGSIMEFAAVEACPVI
                     DTDLKLVGMISDRDLIKASVIEDSVEKTDMSADGGEDAWMWDRVMRTINKYYTVSRIK
                     LRDIHVREAMVPPKTAFKTDKVSDCAAVMRKNRIDQMPVVKTSRKLMGMLKDTDI"
     assembly_gap    3150..3249
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3405..4754
                     /gene="thrC"
                     /locus_tag="PLV49_03505"
     CDS             3405..4754
                     /gene="thrC"
                     /locus_tag="PLV49_03505"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOG97943.1"
                     /translation="MSLIKFFSTNGHLDRVDFKGALLAGQAPDKGLYMPEMFPSLSPE
                     TIRNFPQMSYHQIAYQVIKPYLGDLVEESALEEMLKDAYSYEVPLERVYDGKYLLRLD
                     RGPTCSFKDFAARLMGRLMQYFLKEEGRSMIILTATSGDTGSAVAHAFYGLENIKVIV
                     LFPEKEVTERQRRQMTTLGENIYPLAVTGKFDDCQALVKQAFVDPELKGLELSSANSI
                     NVGRLLPQAVYYFYAHSRVAPGGEEVVFSVPSGNFGDLMGGLIALKMGLPVKKFVAAT
                     NENDEFPRFMQTGVYEPIRPSRNCISNAMNVGHPSNLARLFSLYGGQMDETGQVARQP
                     DLSAMREEIYAVSISDEETRQTIREAYSQYKVLLEPHGAVGWAGLVRYLKEFGDWSPA
                     VSLETADPAKFPDEIVRLTGVNPSLPPAMASLDEKEEHYLKMDGSYASLKDYLKGFF"
     gene            4776..>5252
                     /locus_tag="PLV49_03510"
     CDS             4776..>5252
                     /locus_tag="PLV49_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HOG97944.1"
                     /translation="MEQERIFFDDIGSYPLPRGVRLDGLSRDRYLQLVGQVLAEKIHA
                     GVEIPTYPQLRDMIRMFMDPIKDPKTTESPFLIRREEAKIMELEAVPPGQKVRVCVTG
                     PVELYISAFGATGYTDILYTLAESVSRFLEGAKEEGKMAVASLDEPSLGLNSNIIFS"
BASE COUNT         1211 a         1335 c         1495 g         1111 t
ORIGIN      
        1 tttcggtgaa ccctttaatg aagcttccac tgaagcagga ggccctcatc cagcctctga
       61 acagactgga ggctgagcct gggaaaggaa ttccgaaatc cagggccatc taccaggctg
      121 ggtgccctct ctccccctat tatcagccct cccatgtaga tgtaaatctc atcgaccaga
      181 ccaagctcta tcaacgacca gttgaggcgg gcgccgcctt ccaccataag gcgccttacc
      241 ccccggttat gcagctcttc catgagccct ggcaggtcca ccatctcggt gccgcatttg
      301 attatctcac agccaggtcg aagcctatcc agtctctcct caggggcaga ataggatact
      361 gcgatcatgc acccaggccc gaggatcttt gcattggccg gagttcgagc gttgctgtcc
      421 gccacaattc ggaagggctc tttcgactgc cccatagccg ttctcttctc ctgcaagatc
      481 ctggatttga ccctcagccc gggatcgtct gccagaaccg tccccacccc caccatgatg
      541 gcatcgcttg cagcccgcag ctcatctacc ctcattagat cctctttgcc cgagatcctt
      601 atctgtctgc ggtctcttga gctgatcttg ccgtccgcgc tcatggcgct gtttatgaaa
      661 acaaatggcc tgttcaaatg atctccgccg tcatattctc gccgtattct tattctgatc
      721 cttttgttct actatgagaa gctaaataca ttgcctcgtc ttataaccat agccatgtca
      781 aatatcgcca agcccaaagt agctccgcct ggtgcagcca ggctcaaaca ggatgagctc
      841 attctgcagg gaaccataat ctatggcgat gatttccagc ccattgaggg ctacatcagc
      901 atccgggacg gaatcataaa ggaagcgggc accggtcaag tggacgccga ttttgagggg
      961 atagtctgcc ctcgtttcgt caattctcac attcatctgg gcgactcggc attcaaggat
     1021 cctccattcc tccccctctc cgagcttgtc ggcccaaaag gcctgaaaat aagcctgctc
     1081 tgtgagacgc cgcgtgagcg tctcattgag ggcatgagaa gaagcctggc ccagatggtg
     1141 gactcgggca cctatgcctt tgctgacttt cgcgaggggg gcgaggcagg agtcaggatg
     1201 ctgcaggagg cggcgaagga tatgcccctc ctcgtgcgcg tctttggccg gccagcacag
     1261 ggaaagacgg agacgccgga aggctgcact ggcctgggaa taagcagcac cagggatttc
     1321 gatcccagcc ttatccagag aatggtcttg gaggcgcgcc gggcgagcaa ggcggtggcc
     1381 attcatgcgg gcgagtcagc cagagacgat ataccggatg ccttctcact cttgcccgat
     1441 catctggtgc atatgagccg agcagaggag tctgacctca gatctgtggc ccaaaagggc
     1501 actcccctgg tgatctgccc tcggtcgaat ctgatcaccg gctcaggcct tccaaatgtg
     1561 aagaggatga ttgaactggg gataactgtt ggagtgggga cggacaatgt gatgctcaac
     1621 tccccgaata tgttcatgga gatgcatctg atcagcaggg ctttgctgca tgacgataga
     1681 caggtattta agatgtgcac tctaaatggc gctgaaataa tggggcttga tcagaagctc
     1741 gggtccataa gggaaggaaa agaggcaaag gtgatggtca tcaacggaag ctcagacaac
     1801 ctttggggat cgactcatcc tctccgcagc attgtgcgga gggccgagcc aactgatata
     1861 ttagcgatct tctagcttta attattgggg tggatcatgt tcgagaagat tctagcggca
     1921 accgatggat cggagcacgg attgagagcg gcaaaagtag cagtggaact gggaaagatc
     1981 tcaggaggaa aggtctttgc cgtctatgta gcggacacgc aaaggacagc tcatctgccc
     2041 gatgatatgc tgctgttcag catcaaagag cttctgctca aagagggaaa ggaagcgata
     2101 aaacaggtcc aggagctggc agagaagaat ggcgtcgact tcgatggagc agtggtggaa
     2161 ggcaatccca gcgatgagat cattagctat gctaagaaaa agggggcaaa tgtaatcatc
     2221 gtcggcgctg tgggacgcac tggccttgac aaattcctcc tgggaagcgt ggctgagaag
     2281 gtggtacgca actccaggat tcccgttttg actgttccga gggaagagtc ttgaagattc
     2341 ttgtcagaga tgtgatggtt cgggaggtcg cctatgccga gatccccgga aacagggacg
     2401 atgtcttgaa ggcccttcaa gaccggaagg tctccggcct gccggtggtg aagaaaggag
     2461 aggtggtggg gatcataacc cgatccgatc tgctccgcaa tcgggaggag gaccagactg
     2521 ctctgctcat gacccgcgat ccggttgtga taagcccgga caggagcata gttgaggctt
     2581 caaagcttct tatccagcat aagataaggc gccttcctgt ggttgagggc aaagagctgg
     2641 tggggctggt cacagtggcg gacatcgtcc gggtggcaga gggcatgaac atagaggaga
     2701 gcatagagcc gtacctggag aaggaaacgg ttgtactctg gagcgagatg cccctgccgg
     2761 tagcaggatc gatcatggag tttgcggctg tcgaggcctg tccggttata gataccgacc
     2821 tgaagctggt gggcatgatc tctgacaggg atctaatcaa ggccagcgtc atcgaggatt
     2881 cggtggagaa gacggacatg tccgcagacg gtggggagga tgcctggatg tgggaccggg
     2941 tgatgcgaac catcaataag tattacaccg tctccaggat aaagctgcgc gatatacacg
     3001 tacgcgaggc aatggtcccg ccaaagacgg cattcaagac ggacaaggtg agcgactgcg
     3061 ctgctgtgat gcgcaagaac cggatcgatc agatgccggt ggttaaaacg agccgcaagc
     3121 tgatgggaat gctcaaggat accgatatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnng ccggtggtta aaacgagccg caagctgatg ggaatgctca aggataccga
     3301 tatcctgaga gctctggtgg actattgcga aagatcaagc gcttgaattc aatcggtcaa
     3361 atggcgaaga tttaaagatc aggccatccc aaccacaaag gactatgtct ctaatcaagt
     3421 tcttcagcac caacggccac ctggataggg tggactttaa aggggctctc ctggctggcc
     3481 aggccccgga taaggggctc tatatgcccg agatgttccc ttctctctcc ccggaaacca
     3541 tccggaattt tcctcagatg agctatcatc agatcgcata ccaggtcatc aagccatatc
     3601 tcggcgacct ggtggaagaa tcggctctag aggagatgct aaaagacgcc tacagctatg
     3661 aggttcccct ggagcgggtc tatgacggca agtacctgct gcggctggat cgcggtccta
     3721 cctgctcatt caaggacttt gccgccaggc tgatgggcag gttgatgcag tacttcctca
     3781 aagaggaggg aaggagcatg attattttaa ctgccacctc tggggacacc ggctctgcgg
     3841 tagcgcatgc attttatggc ctggagaata tcaaggtgat agtgctattc ccggaaaaag
     3901 aggttacgga aaggcaacgc cggcagatga ccacacttgg cgagaacatc tatcctctgg
     3961 ccgtaaccgg caagttcgat gactgtcagg ccctagtcaa gcaggcattt gtcgatccgg
     4021 agctcaaggg actcgagctc tcctcagcca actccataaa tgtcggccgg ctgctgcccc
     4081 aggcggtcta ttacttctat gctcattccc gggttgcacc cggaggagag gaggtggtct
     4141 tctctgttcc cagcggaaac ttcggcgacc tgatgggcgg tcttatcgct ctgaagatgg
     4201 ggcttcctgt gaaaaagttc gtggcggcca ccaatgagaa cgacgagttt cctcgcttca
     4261 tgcagaccgg cgtctatgag ccaatccggc cgagcaggaa ctgcatctcc aatgccatga
     4321 acgtcggcca tcccagtaac cttgcccggc tcttctccct ttacggcggc caaatggatg
     4381 agaccggaca ggtagccagg cagccggacc tctccgccat gcgcgaggag atctatgccg
     4441 tatctatcag cgatgaggag acccgccaga ccatcagaga ggcttactct cagtacaaag
     4501 tcctcctcga gccccatggg gcggtgggat gggcggggct ggtgaggtat ctgaaagaat
     4561 tcggagactg gagccccgca gtctccctgg agacggcaga tccggccaag ttcccggatg
     4621 agattgtgcg cctcacaggg gtgaatccct ctcttcctcc agccatggcc agtctcgatg
     4681 agaaagagga gcactacctg aagatggatg gcagctacgc ttcactgaag gattatctga
     4741 agggcttttt ctgatcgatt actggaggca ataggatgga gcaggagaga atattcttcg
     4801 atgatatcgg cagctatccc ctgcccaggg gagttaggct ggatggcctg tccagggatc
     4861 ggtatctgca actcgtgggc caggttctgg ccgagaagat ccatgccggg gtggagattc
     4921 ccacctatcc tcagctccgg gatatgattc gcatgttcat ggaccccatt aaggatccga
     4981 agacgacgga aagccccttt ctgatcagga gagaagaggc gaagataatg gagttggagg
     5041 ccgtccctcc cggtcagaag gtgcgggtct gtgtcacggg accagttgag ctctacatat
     5101 ccgcttttgg ggcaacgggt tacactgata ttctttatac cctggcggag agcgtttcca
     5161 ggttcctgga aggggcaaag gaagagggaa agatggccgt cgcctcactg gacgagccga
     5221 gcttgggcct gaactccaat atcatcttct ct
//
LOCUS       DAOSGP010000079         5020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_14878_length_5020_cov_19.413494, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000079 DAOSGP010000000
VERSION     DAOSGP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14878_length_5020_cov_19.413494"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..321)
                     /gene="ffs"
                     /locus_tag="PLV49_03515"
     ncRNA           complement(7..321)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLV49_03515"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            378..632
                     /locus_tag="PLV49_03520"
     CDS             378..632
                     /locus_tag="PLV49_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOG97945.1"
                     /translation="MADDMDAIMGFIMGNKQRERIVQILGSKGKMESDKVAKVTHIPA
                     PSVKKILAEMAERDLVREENGIWGLTGAGAEIEKELKKRG"
     gene            complement(641..1612)
                     /locus_tag="PLV49_03525"
     CDS             complement(641..1612)
                     /locus_tag="PLV49_03525"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HOG97946.1"
                     /translation="MRSLKDRIRESGRVFVFGIAGDSGSGKTTISRGIRRILGEDMVA
                     TFSMDDYHSLDRRQRKERNITPLHPDANQFDLLAEHLEALRRNERIDKPVYDHRTGEI
                     SGTVPFGPAPVIIVEGLHPLFTERLRNQIDFKIFVDPSRSVKRLWKIRRDVGERGYET
                     GQVMAEILQREPDYKLYVDIQKIYAQIVIKIQDSRFHPSLLDSQSTLDWYSVRLIMEI
                     MEHPVSEVSMNIDLSKILRRSEHEFSIEFQRDDYYGKRVGIMTMDGEIHQSMISDLEK
                     KLGESLGTDGMISDRREEYVNAIGLAQLILTWNCVEKLDYLLQKEGY"
     gene            complement(1685..2614)
                     /locus_tag="PLV49_03530"
     CDS             complement(1685..2614)
                     /locus_tag="PLV49_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOG97947.1"
                     /translation="MMDKELLAAHVEEVLREAGFSTSDRCYLRPRSFDLAARRDDQFL
                     FLKILSNIDGLNERTALEIRRLAKHLLASPILVGEKTRDQYLERGAVYFRYGIPTLSL
                     FTLADCLLEGALPLVYAAHGGLYVRIDGDRMRQIRLARGISLGALATELGVSRRTISK
                     YEVEDMDTSVDVALKLEEIFNEELIEPVDPFQTEAVAEVKGPVTDNILRLLLEIGFEV
                     VPTAQAPFNAITREGKLVVLTGVGKFSQSIIKKARLMSSLSSVARTKSVVIVDGETKL
                     ECIEETVLIEKRELEDIDETRDFEDLVAEKKSK"
     gene            2651..3922
                     /locus_tag="PLV49_03535"
     CDS             2651..3922
                     /locus_tag="PLV49_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HOG97948.1"
                     /translation="MFIGIDDTDSERGLCTTYLVGVLMERLASWGEIEGLPRLIRLNP
                     CARYKTRGNAALAFSLKSSRPDMVKRIALQTLLELSDFSGSNTNPGLVIAEEVTERMR
                     AFYRSAVTEILSIDAARSLLDEEGIWYRGFKKGRGLIGALAAVGADLPDWTYELIAYR
                     QRSQWGTPRFIDPATVWEADRLTYPQTWDTVDHHNHRVVFAPHSADPVLFGIRGSDPV
                     AIKRAFETIRSEPVERYVLYQTNQGTDAHILGGQIGRTKDSQSYRLHGFVAGPARAIA
                     GGHVFLTLQNEEMEPNEGGRIECAAFEPTKNFRNIIKELRPGDELEVFGSVRDGTLNL
                     EKINILHLAPEKVQAAPLCPQCHRRMESAGKGQGYRCRRCKTRSSSREIENRARDLET
                     GFYEVPPCARRHLSMPLVRLKQKDLCIHPSR"
     gene            complement(4053..>5020)
                     /locus_tag="PLV49_03540"
     CDS             complement(4053..>5020)
                     /locus_tag="PLV49_03540"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HOG97949.1"
                     /translation="FYINNAEVLRLTDKIYVIGHKSPDTDSVTSAITYANLKNALGMK
                     EAVPAAAGDINAETKFLLDHFKVPYPEKLTDATDKKVILVDHNEMAQAVDNLNPENIV
                     EIVDHHKIGGLATGKPIFFLNEPVGATGGIIANLYEMNNVPISKEMAGLMMSAILSDT
                     VLFKSPTCTPRDKTAVEKLAKIAGVDPMNFGMELLKAKSDVSSKSAKDILLGDFKKFD
                     FSGTKSGVGQIEVMDLKDLEPKRAAILEEMNKMKDAEGLDMIVLMLTDVMKEASDILF
                     VGNAQAAAGFEKAFGGKIQNNSIYKEKVLSRKKQVVPPLEGAFKK"
BASE COUNT         1094 a         1409 c         1354 g         1163 t
ORIGIN      
        1 attaaatggc gagcacgccg gcagttgccc ccaggcttat gcgccagttt ttcccgatgc
       61 ttcctcctcc ccttacacct ataagcgaca gttacccgca gtgggtctgc tcccttccgg
      121 gcctgaaccg gttcctgcaa ttatggtaca cgggtcattc ctcagaagga cccatggacc
      181 ggtgctatcc ctacaggacg gagaatcata gttggcagct ggactgaacg cacagcgccc
      241 gaggtcttcc gtcagcttcg ccccccgcgt atcgacgatt tcgggttata ggtgacgccg
      301 aaccacccgg actgcccgcc acttttaaca ttgaggtgta cagctaataa agctaacgca
      361 gaaaagaggt gcaaaatatg gcagatgaca tggatgcaat tatgggtttc atcatgggaa
      421 acaagcagcg ggaaaggatt gtccagatcc tgggctccaa ggggaagatg gagtcagata
      481 aggtggccaa ggtcactcat atccctgccc cgtcggtcaa gaagatactg gcggagatgg
      541 cagagcggga tctggtcaga gaggagaatg gcatctgggg cttgaccggg gcgggagcgg
      601 agatagaaaa ggagctcaag aaaagaggct gagtgagcct ctaatagccc tctttctgga
      661 ggagatagtc cagcttctct acgcagttcc aggttaagat gagctgagcc aggccgattg
      721 cattgacgta ctcctccctt cggtcgctta tcatgccgtc cgttcccagg gactctccca
      781 gcttcttctc caggtcagag atcatggact gatgaatctc cccgtccatg gtcattatcc
      841 ccactctttt cccgtagtag tcatccctct gaaactcaat ggaaaactca tgctccgatc
      901 ggcgcaggat cttggagaga tcgatgttca tgctcacctc ggacacggga tgctccatta
      961 tctccatgat cagccgcacc gagtaccagt ccagggtgga ctgggaatcg agtagagagg
     1021 gatgaaaacg ggagtcttga atcttgatca caatctgggc atagatcttc tggatgtcca
     1081 catagagctt gtagtccggc tcgcgctgta gaatctctgc catcacctgt cctgtctcat
     1141 agccccgctc gcccacatct ctcctgatct tccacagcct tttcaccgac cgggagggat
     1201 ccacgaatat cttaaagtcg atctggttcc tcagccgctc ggtgaagagg ggatgaaggc
     1261 cctctacgat tatcaccggc gccgggccga agggcaccgt ccctgagatc tctccggtgc
     1321 ggtggtcgta gaccggtttg tctattctct catttctccg cagggcttcc agatgctcag
     1381 ccaaaaggtc gaactgattg gcatcggggt gcaagggggt gatgtttctc tcctttctct
     1441 gccttctgtc caaagagtgg tagtcgtcca tggagaaggt ggccaccata tcctccccca
     1501 ggatccttct aatgccccgt gagatggtgg tcttacccga tccgctgtcc ccagcaatgc
     1561 caaatacgaa tacccgtccc gattccctga ttctgtcctt gagcgatctc atgagagcac
     1621 aatgcctcca gaatgcaatt ctggctaaga aatagatgaa tgtatgcctt ggatgattta
     1681 aggcctattt gctcttcttt tccgccacca gatcctcaaa atcccgggtt tcgtcgatgt
     1741 cctcaagctc ccttttctct atgaggacgg tctcctctat gcactccagc tttgtctcac
     1801 cgtccactat caccacagac ttggtgcggg cgacggagga gagactgctc atcagccggg
     1861 ccttcttgat tatgctctgg gagaacttgc ccacgcccgt gaggaccacc agttttcctt
     1921 ccctggttat ggcattgaat ggcgcctgag cggtgggaac cacctcaaaa ccgatctcca
     1981 gcagcaagcg caggatgttg tccgtcaccg gccccttcac ctctgccacc gcctcggtct
     2041 ggaaggggtc taccggctcg atcagctcct cattgaagat ctcctccagc ttcaaggcca
     2101 catccaccga ggtgtccatg tcctctacct cgtacttgct aatggtcctg cgggagacgc
     2161 caagctctgt ggccagggcc cccaaagata tgcctcgtgc cagccggatc tgtctcatcc
     2221 ggtctccatc gatgcgcaca taaagcccgc cgtgcgccgc atagaccagg ggcaaggccc
     2281 cttccagcag gcagtctgcc agggtgaaca ggctgagagt gggaatgcca tagcggaagt
     2341 agaccgctcc cctctccagg tactggtcgc gggtcttctc tccgaccagt atggggctgg
     2401 ctaataggtg cttggccaga cgcctgatct ccagggcagt cctctcattc aggccgtcga
     2461 tattggagag gatcttcaaa aacaggaact gatcgtctct tctggctgcc aggtcgaagc
     2521 tcctgggacg gagatagcag cggtcagagg tagagaatcc tgcctctctc aaaacctcct
     2581 ccacatgcgc ggcaagaagc tccttatcca tcattatata tgtccgtttg accgctattt
     2641 aagcttgacc atgtttattg gaatagatga taccgattct gaacgaggcc tgtgcaccac
     2701 ttatctggta ggggtgctca tggagaggct cgcttcttgg ggggagatcg agggcctgcc
     2761 caggctcatc cgcctcaacc cctgcgcccg ctacaagacc cggggcaatg ctgccctcgc
     2821 cttcagcctt aagagcagcc gcccggatat ggtgaagaga atcgccctcc agacgcttct
     2881 cgagctttcc gacttctccg ggtccaacac caatcccgga ctggtgattg cagaggaggt
     2941 gacggagagg atgagggcct tctatcgctc tgcggtcacg gagatcctct caatagatgc
     3001 cgcgagatca cttctggacg aagaggggat atggtaccgg ggcttcaaga agggaagggg
     3061 tctgatcggc gctctggctg cggtgggggc agacctgccg gactggacct atgagctgat
     3121 agcctatcgg cagcgcagcc agtggggcac gcccaggttc atagatcccg ccaccgtatg
     3181 ggaagcggac aggctcactt atccccagac ctgggatact gtggaccatc ataaccatag
     3241 agtggtattc gctccccatt ctgctgatcc agtcctcttc ggcataagag gctcggatcc
     3301 agtggccatt aaaagagcat ttgagaccat aagatctgag ccggtggaga ggtatgtgct
     3361 ctatcagacc aaccagggca cagatgccca tatcctcggc ggtcagatag gccggacaaa
     3421 agatagccag agctaccgtc tgcacggttt cgtcgccggg ccggccaggg cgatcgctgg
     3481 tggacacgta ttcttgaccc ttcaaaatga ggagatggag ccgaatgaag ggggcaggat
     3541 agagtgcgca gccttcgagc ccaccaagaa ctttcgcaat atcataaaag agctgcggcc
     3601 gggggatgag ctggaggtct tcggttcggt gcgggatgga acgctgaacc tggagaagat
     3661 caacatcctc catcttgctc ccgaaaaggt acaggcagcg cccttatgcc ctcaatgcca
     3721 tagaaggatg gagtccgcgg gcaaaggcca gggctaccgc tgccgtcgct gcaagaccag
     3781 atcatcctct cgcgaaatag agaatcgggc ccgtgatctt gagaccgggt tttatgaggt
     3841 gcctccctgc gctcgccgcc atctctcaat gcctttggtg cgcttgaaac agaaggatct
     3901 atgcattcac cccagccgct agaccacttc aactgtgagc aatcagttgc aggacggtgg
     3961 cgatttaggc gggatggtgc gctggcaaag ggaaaagaat gcaaaaaaga gaaaaactaa
     4021 aggtggccca tcggccacca aatccagatg atttatttct tgaaggcgcc ttccaggggc
     4081 gggacaacct gctttttcct ggagagtacc ttttccttgt agatggagtt attctggatc
     4141 ttgccgccga atgccttctc aaatcctgcc gctgcctggg catttcctac gaagagaatg
     4201 tcgcttgcct ctttcatgac atcggtgagc atcagaacga tcatatccag accttcagcg
     4261 tccttcatct tgttcatctc ttccagaatt gccgctctct tgggctccag gtccttcaga
     4321 tccatgacct cgatctgacc aacgccggac tttgtgccgg agaagtcgaa cttcttgaag
     4381 tctcccagga gaatatcctt ggccgacttg gatgagacat cgctcttggc cttcagcagc
     4441 tccataccga aattcatcgg atcgacgcca gcgattttgg ccagcttctc aacggctgtc
     4501 ttgtcgcgag gagtgcaggt gggcgacttg aataggacgg tatctgagag gatggcggac
     4561 atcatcaggc cagccatctc cttgctgatc ggcacattgt tcatctcata gaggttcgcg
     4621 atgatgccgc cagtcgcgcc caccggctca ttgaggaaga atatgggctt tcctgtggcc
     4681 aggcccccta tcttgtggtg gtccacgatc tccacgatgt tctcaggatt gagattgtcc
     4741 accgcctggg ccatctcatt gtggtctacc agtatcactt tcttgtctgt agcatcggtg
     4801 agcttctccg ggtaaggaac cttgaagtgg tccagcagga acttggtctc ggcatttatg
     4861 tctcctgcgg cggcaggtac ggcctctttc attcccaggg cgttcttcag attggcatag
     4921 gttatggcag atgttacaga atcggtatca ggactcttgt gcccgattac atagatcttg
     4981 tcggtcaaac gaagcacctc agcattgtta atgtagaaca
//
LOCUS       DAOSGP010000080         4968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_15081_length_4968_cov_12.920415, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000080 DAOSGP010000000
VERSION     DAOSGP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15081_length_4968_cov_12.920415"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLV49_03545"
     CDS             <1..482
                     /locus_tag="PLV49_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97950.1"
                     /translation="RLKGPPVETIKLEAEIDAADQMEFGDQDPIIEFGIHPQIAALES
                     MLYPTSRSMQENHDMAASGQLQIIPMESPLALFVWSKSRIVPVRITDLSVTEEAFDPN
                     LNPLRAKVSLGMRILSVDDLGFEHKGGSIYMTYQKEKERLASLSHSGTLSSFGITGV"
     gene            482..775
                     /locus_tag="PLV49_03550"
     CDS             482..775
                     /locus_tag="PLV49_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97951.1"
                     /translation="MTNDNFSPTSRYYRTETALMKTAEGREVVYLRRRFLPLDTGQLL
                     TEHVVTQGDRLDNITAGYLGDPEQFWRLCDANLAKNPQELTSEIGRRLRIPAP"
     gene            787..1896
                     /locus_tag="PLV49_03555"
     CDS             787..1896
                     /locus_tag="PLV49_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97952.1"
                     /translation="MSDQGIRLQLLIGRNDPRPAPPQVIDSLTSLEVTNNDRERDGFQ
                     LSFTLGRDSLAEYGLLQSGLLEPPNRLIASVTVRGQSYVLIDGVITNHQTAPSSDPGR
                     STLSVTGDDISLHLDLEEKNATYPNQSDSVIVQRILRSYAEFGLNPDVKETKEAPSET
                     DRLTTQQGTDLSFVQELARRNGFVFYIEPTKPAESTAYFGPENRLGQPQPALSMNMGP
                     DSNLDGLNITYNALGPTDPQAKMVDMPTRQSFSVPLPSGLPEPISFQPAAPLRRTIVR
                     NTAHLNSIQAGLRTLTSANQSANAVTATGELNVVRYGRVLQARRLVSVRGVGNSNDGT
                     YYVKQVTHRLSRGEYKLSFTLEREGRGSLSRRVIS"
     gene            1893..2375
                     /locus_tag="PLV49_03560"
     CDS             1893..2375
                     /locus_tag="PLV49_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HOG97953.1"
                     /translation="MTEFFGKYRGVVTDNRDPLLQGRLRATVEDVFGEEECGWALPCV
                     PYAGNGVGFFMLPPVGAMIWVEFEHGDPDYPIWSGCFWAEGESPPPAQPETKVIKTDA
                     GTILVDDTPGAGGITIETANGMKIVLSELGIEIDDGQGGSIKITGQTVSVNDGALEVT
                     "
     gene            2376..2693
                     /locus_tag="PLV49_03565"
     CDS             2376..2693
                     /locus_tag="PLV49_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97954.1"
                     /translation="MPGYLLHQGATVMCMHAGQAEPILVEPRVRVMGQPIVTQASTYL
                     IAGCTLASIPSPACVSAQFASASSRVRAGDLPVLLQDSIAVCVPTGTGLNIVLAQMRV
                     KGI"
     gene            2690..3055
                     /locus_tag="PLV49_03570"
     CDS             2690..3055
                     /locus_tag="PLV49_03570"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOG97955.1"
                     /translation="MMNIDYPFQFDGRGRTAETVDADHIRDLIEQFIFTAAGERVNRP
                     DFGSGILQMVFAPNSPELASALQHTIKSGLQRWMGDLINIQSLEVSRDGSKLQVVIRY
                     TILRTSESHEDTLVMGGLS"
     gene            3052..>4968
                     /locus_tag="PLV49_03575"
     CDS             3052..>4968
                     /locus_tag="PLV49_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97956.1"
                     /translation="MTGKGFVCTNDSRHEKVRQRSLEENPVYGLDHLEVASPDQKTLH
                     VYFIGRAPCHLRKENLVIEGGLRVRDIRVSNSEMLRNGDSEEPYLKVRVDRMGDFSNY
                     VLKVVDLDEEGKPTDKPMKNFDPCYASLPFTFKAACPSRLDCRPPDICKALKFADPEI
                     NYLAKDYASFRQIILDRLALILPDWKERHVPDLGIALVEILAYAGDHLSYYQDAVATE
                     AYLNTARQRISVRRHARLVDYRMHEGCNARTWVFLETSDDLPKGEEQIFYLRDIYFLT
                     RLSEGRFLEGAVLKEDDLRDIPRSSYEVFEPLEDNDFSLHESDINLENLTARLSKEDY
                     GKKEYKRKKHKDVLSYHILQRLSDESRNLMRSFVLTGSSREELQTALSRDLNRIMQSS
                     LYNSDLFPDGDLRDGTKELIDEALRGDELVRFNRMLLEDAFEGISKKGVLRIWEAHNC
                     ISIYTWNRRECCLPRGATRATLLDDWLEEKDEIIPEASSSVQSKKIQTSGPSTTAARR
                     RRLNLKKGDYLLFEEVKGPRTGEKADADPSKRVVVRLTRVTPLVDPIFDQPVLLVEWS
                     RKDALPFSLCISAVGRPKECELIQEITVARGNLVLVDHGRRIKKEALGSVPEKERVRS
                     CTAERLPADTAIEAA"
BASE COUNT         1152 a         1377 c         1426 g         1013 t
ORIGIN      
        1 tgcgcctcaa gggcccgcca gtggaaacca taaaactgga ggcggagatc gatgccgcag
       61 accagatgga gtttggcgat caagatccga tcatagagtt cggcatccat ccccagatcg
      121 ccgccctgga atccatgctc tatccaacga gccgctcaat gcaggagaac catgacatgg
      181 ctgcctccgg gcagctccag ataattccta tggagtcgcc tctggcactc tttgtctgga
      241 gcaagagccg cattgtgccc gtgcgcatca ccgaccttag cgtgaccgag gaggcattcg
      301 accccaacct caaccctctg cgggcaaagg tctccctggg catgcggatt ttgagtgttg
      361 acgatctcgg atttgaacat aaaggaggaa gcatttatat gacctatcag aaggagaagg
      421 aacgtctggc ctccctgagc cacagcggca ctctcagctc ctttggcata acgggggttt
      481 gatgaccaac gataatttct cgcccaccag ccgctattac agaaccgaaa ccgctctcat
      541 gaagaccgca gaaggcaggg aggtggtcta tcttcgccgc cgctttcttc ctctggatac
      601 gggtcagctg ctgacggagc acgtggtgac tcagggcgac cggctggaca atatcaccgc
      661 cggctacctg ggagatcctg agcagttctg gcgcctctgc gatgccaacc tggccaaaaa
      721 tccccaggag ctgacttcag agatcggccg aagattgagg atccctgcgc cttaggaagt
      781 tgagccatgt ccgatcaagg aatccgtctg cagcttctca tcggccgcaa tgatcccagg
      841 cccgctccgc cccaggtgat agactctctg accagccttg aggtgaccaa caacgaccgg
      901 gaaagggacg gcttccagtt gagcttcacc ctgggcaggg attccctggc cgagtacggc
      961 ctgctgcaga gcggcctgct ggaacctccc aaccggctca tagcttccgt caccgttcgc
     1021 ggtcagagct atgtgctgat agacggcgtg atcaccaacc atcagaccgc tcccagcagc
     1081 gatcccggtc gatccacgct atcggtaact ggagatgata tcagcctgca tctggatctg
     1141 gaggagaaga acgccaccta tcccaaccag tccgattcgg tgattgtgca gaggatactc
     1201 cgctcctatg cagagtttgg cctgaatcca gatgtcaaag agaccaaaga ggctccttct
     1261 gagaccgatc gcctgacaac acagcagggc actgacctct ccttcgtcca ggagctggcc
     1321 aggcgcaatg gctttgtctt ctatattgag ccgacaaagc ccgcagagag caccgcctac
     1381 tttggcccgg agaatcgatt ggggcagccc cagccggcat tgagcatgaa tatggggccg
     1441 gacagcaacc tggatggcct gaacatcacc tataacgccc tcggtcccac agatcctcag
     1501 gcaaagatgg tggatatgcc caccaggcag agcttttcgg ttcccctgcc ttccggcctt
     1561 cccgagccca tctcctttca gccggcagca cctttgcgtc gaaccatcgt ccgcaacact
     1621 gcccacctca actccattca ggccggtctg agaactctca cctctgccaa tcaatcagcc
     1681 aacgccgtaa cggctacagg tgagctgaat gtggtgcgct atggccgggt gctccaggca
     1741 cgccgcctgg tttcagttcg gggcgtgggg aacagcaacg atggcaccta ctatgtgaag
     1801 caggtaaccc accgcctgag ccggggcgaa tacaagctga gcttcacctt ggagcgagag
     1861 gggcgggggt ccttgagcag aagggtgatt tcatgacgga gttcttcggc aagtaccggg
     1921 gcgtggtcac tgataaccgc gatccactgc tgcaaggaag gctgagggcc acggttgaag
     1981 acgtctttgg tgaagaagag tgcggctggg ctcttccttg cgtgccctat gccggaaacg
     2041 gtgtgggatt tttcatgctt cctcccgtcg gagccatgat ctgggtggag ttcgagcacg
     2101 gcgacccgga ctatcccatc tggtcaggat gcttctgggc agaaggggag tctcctccgc
     2161 cggcccaacc ggagacgaag gtcatcaaga cggacgccgg aaccattctt gtcgatgata
     2221 cccccggtgc cggaggcatc accatcgaga cggcaaacgg catgaagatc gttctgagcg
     2281 aattgggtat agagatcgat gacggccaag ggggaagcat aaagatcacc ggccagacgg
     2341 tctcagtgaa tgatggcgcc ctggaagtga catagatgcc cggctatctt ctgcatcaag
     2401 gcgctacagt catgtgcatg catgccggcc aggcagagcc cattctggtc gagcccaggg
     2461 taagggtcat ggggcagccc attgtcaccc aggcgagcac ctacctcatc gctggatgca
     2521 cactggccag cattcccagc cctgcctgcg tcagcgccca gtttgcgagc gcctccagcc
     2581 gggtgagagc gggtgatctt ccggtgctgc tccaggacag catagccgtc tgcgttccca
     2641 ctggaacagg actgaatatc gttcttgctc aaatgcgtgt aaaggggata tgatgaatat
     2701 agattatccg ttccaattcg atggccgggg gagaacggcc gaaaccgtgg atgccgatca
     2761 tatcagagat ctgattgaac agttcatctt cactgccgca ggagagcggg taaaccggcc
     2821 cgattttggg agcggaatcc tgcagatggt cttcgcacct aacagcccgg agcttgcttc
     2881 tgccctccag catactatca agtccgggct tcagcgctgg atgggagatc tgataaatat
     2941 tcaatccctt gaggtcagcc gggacgggtc caaacttcag gttgtgattc gttatacgat
     3001 actgcgaacc tcggagtcgc acgaggatac attggtcatg ggaggcctgt catgaccggc
     3061 aaaggatttg tctgcacaaa cgacagcagg cacgagaaag tgaggcagcg cagcctggag
     3121 gagaatcccg tctacgggct ggatcatctg gaggttgcca gcccggacca gaagacgctt
     3181 catgtctact tcataggaag agcaccctgc cacctgcgca aagagaacct ggtcattgag
     3241 ggagggctgc gagtaagaga catcagagta tccaatagcg agatgctccg caacggcgac
     3301 tcggaggagc cctatctgaa ggtccgtgtc gaccggatgg gcgacttctc caattatgtc
     3361 ctgaaagtcg tcgatctgga tgaggagggc aaaccgacag acaaacccat gaagaacttc
     3421 gacccctgtt acgcctcgct cccattcacc ttcaaggcgg catgtcccag ccgtctggac
     3481 tgcaggccgc cagatatatg caaggctctg aagttcgccg atccagagat caattatctg
     3541 gccaaggact atgccagctt tcgccagatc atcctggatc gtctggctct gatactgccc
     3601 gattggaagg agaggcatgt tcctgatctg ggcattgccc tggtggagat cctggcttat
     3661 gctggcgatc acctcagcta ttatcaggat gctgtggcga cagaggccta cctgaatacc
     3721 gccaggcaac gaatctcagt gcgccgtcat gcccggctcg tggattacag gatgcatgaa
     3781 ggctgcaacg ctcgaacctg ggtctttctg gagaccagcg atgatctccc caaaggcgaa
     3841 gagcagatct tttatcttcg ggatatctat ttcctgaccc gcctatccga ggggcggttt
     3901 ctggaaggtg ctgtattgaa agaggatgat cttcgagata ttcctcgttc cagctatgag
     3961 gtctttgagc ctcttgaaga caatgatttc tcgctgcatg aatcggatat caacctggag
     4021 aatctgaccg ccagactcag caaagaggat tacggaaaga aagaatacaa aagaaagaag
     4081 cacaaagacg ttctatccta tcatatcctg caaaggctct cagacgagtc tcgcaacctc
     4141 atgaggtcct ttgttctgac gggatcatcc cgggaggagc tgcagaccgc cctctctcga
     4201 gatctgaacc ggataatgca gagcagcctt tacaattcag atcttttccc tgacggtgac
     4261 ctccgtgatg gtaccaagga gctaattgat gaggctcttc gaggagacga gctcgtgcgc
     4321 ttcaatcgca tgcttctgga agatgctttc gaggggatat ccaagaaagg cgtgctgcgc
     4381 atctgggaag ctcacaactg catcagtatc tacacctgga atagaagaga gtgctgcctg
     4441 cctagagggg caaccagggc gactctgctg gatgactggc tggaagagaa agatgagata
     4501 atacctgagg ccagctcatc cgtgcagagc aagaagattc agacatccgg cccatcgaca
     4561 acggcagctc ggcggcgccg tctcaatctc aaaaaagggg actatctcct ctttgaagag
     4621 gtgaaggggc ccagaacagg agagaaggcc gacgccgatc ccagcaagag ggtggtggtg
     4681 cgtctgacca gggtgacgcc attggtggat ccaatcttcg atcagccggt tctgttggtg
     4741 gagtggtccc ggaaggacgc tctgcccttt tccctctgca tctcggcagt tggtcgccct
     4801 aaggagtgcg agctcatcca ggagataact gtggccagag gtaacctggt tttagtggac
     4861 catggccgga ggataaaaaa agaggctctg ggatctgtgc ctgagaagga gagggtgaga
     4921 agctgcaccg cggagaggct gcctgcggat actgccatcg aggcggca
//
LOCUS       DAOSGP010000081         4940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_15200_length_4940_cov_19.202047, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000081 DAOSGP010000000
VERSION     DAOSGP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15200_length_4940_cov_19.202047"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLV49_03580"
     CDS             complement(<1..359)
                     /locus_tag="PLV49_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOG97957.1"
                     /translation="MVLIIPSWLVTLMVAALPVSEVRGAIPLAIGYYGYSWYQAFLIS
                     VLGNLLPVVPLLLFLGPVSDYLRRYTIGDRFFTWLFARTRSKYIQKHENFGLIALIIF
                     VAIPLPMTGAWTGCAIA"
     gene            complement(450..890)
                     /locus_tag="PLV49_03585"
     CDS             complement(450..890)
                     /locus_tag="PLV49_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97958.1"
                     /translation="MVSFKVDADARAIVQGSEDQEKYPIVAKVKGSGKDGALSVQVAG
                     DGSSPISARISGDSQNPIAVEPIKLAPITVVPDLKEVAQTVDFESIIGALTRLSQGVK
                     VNVATSDAPISIALGKIPVDLSISVSSPTQETVFKVEIKGTIGE"
     assembly_gap    1223..1322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1323..2147
                     /locus_tag="PLV49_03590"
     CDS             <1323..2147
                     /locus_tag="PLV49_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HOG97959.1"
                     /translation="KEGKELRRDAALGLCATCSEDGPLLDAARIAAQNLCLLELKMSS
                     IRDPIRLLELIPILKRSGVTLSLRIRPEDLSNTLLQKLNESDLDLIHLDLRGLNGVSP
                     KLVKKAADIRGPAIMALADVGDFEDARTLLAMGADVVSLRGADPEFVQWLAGAMLEYD
                     LLSGWCNAPKHICAGGDLRGLTFCCPPVKNCAVFGALKRAGLSPEEFVNRKLAFARGT
                     PLEKGEGTCFGSLVWCCKISKPCYLRDEALSRAKLSEKEYMELKKKLAEKILQKIH"
     gene            2154..3083
                     /locus_tag="PLV49_03595"
     CDS             2154..3083
                     /locus_tag="PLV49_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HOG97960.1"
                     /translation="MYGSLERSDRVAQCAVLAMLFELSSSPKPGNVDRCHDFSDIRFH
                     HFLTSAVSAYPVFRKAASGKGSPGSLILEGVAAWGDWNLRSNTHFGSLVLLIPLALAA
                     GRMAGHEQGQETKQDESQENGPDDGVDDGLEEELVRVLRSTTVQDAIDFYRAFDLAGA
                     RVVQVDDFSLKDPDWERKLIECNQSLLELMRLSLDHDIVAREWATDFERSFQLAGRLQ
                     EMVSIYGLNDGVVRTFLEALAEVPDSLISAKFGRERAVEVSRMAVDALLDSTLNKARK
                     MDCELNNRDMNPGSTADLIAASLFISLLRRLRF"
     gene            3086..3679
                     /locus_tag="PLV49_03600"
     CDS             3086..3679
                     /locus_tag="PLV49_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HOG97961.1"
                     /translation="MFYGKEALDELGILEGINEIIATTETGGRVNAAPLGIIRSEDSL
                     YIRLFLGTHTYENILTRKWFVANVTHDAWIFAETALEDLPQEYFTHCDGLPILKDTEA
                     WLLFKCDPFASDIIIANIEPVKGELLRKDFRAVNRGANLVVEAAVAATRYMSLRSDSY
                     FEELMKTQRIINRCGNPREREAMERLMDRIDSFAHKR"
     gene            3707..4378
                     /gene="radB"
                     /locus_tag="PLV49_03605"
     CDS             3707..4378
                     /gene="radB"
                     /locus_tag="PLV49_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HOG97962.1"
                     /translation="MSTLERLPVGCQSLDLLLGGGFESGIITELYGESGSGKSNIVLQ
                     LAVAAVARGYRVIFIDTEGFSAERFKQIAGPGAADMAAKIMIFEPMSLEQQAMAIREA
                     SKIAGRDLGLVVLDSATSLYRMLLEAEDSRPARRALTVQLSELQEIARRHRIPVVITN
                     QVYTDIESNSLRPLGGTYLEHMCKAIILLEKTGEGLRRARLMKHRSLPENRTAEFRLT
                     ARGLE"
     gene            complement(4396..4896)
                     /gene="rimI"
                     /locus_tag="PLV49_03610"
     CDS             complement(4396..4896)
                     /gene="rimI"
                     /locus_tag="PLV49_03610"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOG97963.1"
                     /translation="MWPEELFERQHDAGIKEAVLRDAVIIRRMSASDLNAALEIDREV
                     FGGYDPNIFAAFYEYHPATTLAAKVNGELAGFVLGFKHAPFEGRVFWLAIRPRYQGRG
                     IGMRLMLAVLKSFRNLGALGATLEVRVSNRKAQSLYSNLGFQIRGICPSYYSDGEAAF
                     IMKARL"
BASE COUNT         1162 a         1225 c         1368 g         1085 t
ORIGIN      
        1 aaggcaatag cacagccagt ccaggcccca gtcataggaa ggggtatggc cacgaagatg
       61 atcagggcga tcaatccaaa gttctcatgc ttctgaatat acttgctgcg ggtgcgagcg
      121 aatagccagg tgaagaatct atctccaatg gtataacgcc gcaggtaatc ggatacagga
      181 cccagaaata gaagcagagg aaccacggga aggaggtttc ccaggacgga tatgagaaat
      241 gcctggtacc atgagtatcc gtaatatcca atggctaaag gtatggctcc ccgaacctct
      301 gatactggaa gagcggccac catgagagtg accagccacg agggaattat gagaaccatc
      361 cgtatttcct caaaagattg gcaatggcaa tagcttcggc ttcaccgatc tcttctccct
      421 gctcaggcgg catgctgcag atggtcctct tactctccta tagttccttt gatctccacc
      481 ttgaagacgg tctcctgggt gggtgaggag acggagatgc tcaggtccac ggggatcttc
      541 cccagagcaa tggaaattgg cgcatccgag gtggcgacat tcaccttcac accctgagat
      601 agccgggtga gggcgccgat aatggactca aagtccaccg tctgggcgac ctctttgaga
      661 tccgggacca cagtgattgg agccagcttg atgggctcga ccgcaatggg gttttgagag
      721 tcgcctgata tccgggcgga tatggggcta gagccatctc cagccacctg aacgctcaac
      781 gctccatcct ttcccgagcc cttgaccttg gccactatgg gatacttttc ctggtcctcc
      841 gatccctgaa caatagccct tgcatcagca tctactttga agctcaccat catatcacgg
      901 gaagtgattg gattttctcg gatataaagg tggtataggc ccaggcgatg gctcagggca
      961 acagccatgg ataatagtca gaatatggcc cgaataaatg gccacggagg cgaaagcctg
     1021 atatcttatg cgacagtacc ttcccactga tggttgaagt aggatatttg catattgata
     1081 gtcccattgc tgttatttct tccaataacc gtccggagac tgacaatgca gtcggtttga
     1141 tcttgctgga tggtttcgac cttgatatcg tctccccttc tcagatccaa tcccgtctga
     1201 ccgacgtgag gcagaggctc aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnaaagaggg aaaagaacta aggcgcgatg ccgccctcgg cctttgtgca acatgctccg
     1381 aggatgggcc tctcttggat gcggcgagaa ttgctgccca gaacctctgc ctgcttgagc
     1441 tgaagatgtc cagcattcgc gatcccatcc ggttgctgga gctgatccct attttgaagc
     1501 gctctggagt caccctatct ctcaggatca ggccagagga tctctccaat acattgctgc
     1561 aaaagctcaa cgagagcgat ctggacctga ttcacctcga cctgcgcggc cttaatggag
     1621 ttagccccaa gctggtcaag aaagcagctg atattcgcgg tccggcgata atggctctgg
     1681 ctgatgtggg agattttgag gatgccagga ccctcctggc gatgggggca gatgtggtat
     1741 ctctgcgcgg agcggatccc gagttcgtcc agtggctcgc cggggccatg ctcgaatatg
     1801 atcttctcag cggctggtgc aacgccccca agcatatttg tgcgggcgga gacctgcgcg
     1861 gcctgacctt ctgctgtcct ccagtcaaga actgcgcggt gttcggcgcc cttaagagag
     1921 cgggcctctc gccggaggag ttcgtgaacc ggaagcttgc ttttgccaga ggaactcctc
     1981 tggagaaggg agagggtaca tgctttggaa gcctggtctg gtgctgcaag atcagcaagc
     2041 cctgctacct gcgggacgag gcgctatcca gggcgaagct ctccgaaaag gaatacatgg
     2101 aactgaaaaa gaagctggca gagaagatcc tgcaaaagat ccattgatca catatgtacg
     2161 ggtcattaga gagatcggat agagtggccc agtgcgcagt gctcgccatg ctcttcgagc
     2221 tatcctccag tcccaagcca ggtaatgtcg accgctgcca cgatttttct gatatccggt
     2281 ttcatcactt cctgaccagc gccgtctctg cttatcccgt cttcaggaag gcggcgagtg
     2341 gcaaaggaag ccccgggtcg ctcatactgg aaggggtggc tgcctggggg gactggaacc
     2401 tgaggagcaa cacccacttc ggctcgctgg tgctgctgat ccctctcgcc ctggctgcag
     2461 gccggatggc aggtcatgaa caaggtcaag aaacgaaaca ggatgagagc caggagaatg
     2521 gaccggatga tggggtggat gatgggcttg aggaggagct ggtgagagtt ctgaggagca
     2581 ccaccgtcca ggatgcgata gacttctatc gggccttcga tctggcggga gcaagagtag
     2641 tacaggtcga tgatttcagc ctgaaggatc cggactggga aagaaagctg attgagtgca
     2701 accaatccct tctcgaactg atgagacttt ccctggatca tgatattgtt gctcgcgaat
     2761 gggccacgga ctttgagcga tcattccagc tcgctggaag gctgcaggag atggtatcca
     2821 tatacggcct caatgatggc gtggtcagga ccttccttga agccctggcc gaggtgccgg
     2881 acagccttat atccgccaag ttcggaaggg aaagagcggt ggaagtatcc cgtatggctg
     2941 tagatgcgct ccttgattcc accctgaata aggccaggaa gatggattgc gaactgaaca
     3001 acagggatat gaatccgggc tctacagcag acctgattgc ggcatcttta tttatctctc
     3061 ttctgaggag attgcggttt tgatcatgtt ctatggcaaa gaggctcttg atgagctggg
     3121 aatattggag ggcataaacg agataattgc gaccacagag acaggtggca gagtcaatgc
     3181 tgctccttta ggaattatca ggagtgagga tagcctatat atccgcctat ttttaggaac
     3241 tcacacctat gagaatatcc tcaccaggaa atggtttgtg gccaatgtca cccacgatgc
     3301 ctggatcttc gcggagacag ctttagagga cctgccgcag gagtacttca cgcattgcga
     3361 tggcctcccg attctaaaag acactgaggc ctggctgcta ttcaagtgcg atcccttcgc
     3421 ttcggatatc ataatcgcca atatcgagcc ggtaaaaggg gagctattga gaaaggactt
     3481 ccgggcagtc aaccggggag ccaacctggt agtagaagca gcggtagctg ccaccagata
     3541 tatgtccctt cgaagcgata gttattttga ggagttgatg aagacccaga ggataataaa
     3601 ccgctgcggc aaccccagag agagagaggc gatggagagg ctaatggacc ggatcgatag
     3661 cttcgcccac aaaagatagg caattaatga tagaaattcg atttctatga gtacccttga
     3721 acgtttacct gtaggctgtc agagcctgga cctcctcctg ggcggaggat ttgagtcggg
     3781 cataatcact gagctctatg gtgaatcggg atcgggaaag agcaacatcg tcctgcagct
     3841 ggcggtggct gcagtggccc gaggatatcg tgtgatcttc atcgatactg agggcttctc
     3901 cgctgagagg ttcaagcaga ttgccggacc tggtgctgca gatatggcgg ccaagataat
     3961 gatcttcgag cccatgagcc tcgagcagca ggcaatggcc atcagggagg catcaaagat
     4021 cgcaggcagg gatctggggc tggttgttct tgattctgcg acgtcgcttt accgcatgct
     4081 cctggaggcg gaggacagcc ggcctgcccg tcgagctctg accgtccagc tctcggagct
     4141 gcaggagatc gcccggaggc acaggattcc tgtggtcata accaatcagg tctataccga
     4201 tatcgagagc aactcccttc ggccattggg cggcacatat ttggagcata tgtgcaaagc
     4261 gataatcctt ttagaaaaga ccggagaggg cctgcggaga gcacggctga tgaagcaccg
     4321 ctccctgccg gaaaatagaa ctgcagagtt tcgtctgacc gcccggggcc tggagtaata
     4381 gcagacaaga ggcggctaaa ggcgggcctt catgataaat gctgcctctc catcggagta
     4441 gtaagaggga cagatgcctc ttatctgaaa tcccaggttg gaataaagag attgagcctt
     4501 cctgttgctg actcttacct caagagtagc acccaatgca cctaaattgc gaaaggactt
     4561 gaggacggcc agcatcagcc tcatgcctat ccctcggccc tgatatcgcg gccttattgc
     4621 cagccagaag acccgacctt cgaagggagc atgcttgaat cccaggacga agcctgccag
     4681 ttccccgttc acctttgcgg caagtgtggt ggcaggatgg tactcgtaaa aagcggcgaa
     4741 tatgttcggg tcatagcctc caaagacctc tcggtcgatc tccagagctg catttaaatc
     4801 actggcactc attctcctta tgatcaccgc atctctgagg actgcttctt ttatgccggc
     4861 atcatgctgc ctttcgaata gctcctcggg ccacattata ccattgcctg agatttttca
     4921 acggaaatag tttatccgaa
//
LOCUS       DAOSGP010000082         4936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_15218_length_4936_cov_14.405245, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000082 DAOSGP010000000
VERSION     DAOSGP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15218_length_4936_cov_14.405245"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..794
                     /locus_tag="PLV49_03615"
     CDS             129..794
                     /locus_tag="PLV49_03615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG97964.1"
                     /translation="MLTSIIIPTKDEPTIQELVDNINAIIKENHEILIVDKSRTAPKV
                     NGAEVMVQRSDGLGNAFVEGLSRSRGNIIALMDGDGSHRPEDLQKMLKLIENHDIVLG
                     SKLIEGGECSDTLGRILVTVAFAQLTRLILGVDLRDPMTGFMVAKKNVFDNLELKPKG
                     FKIVIEIVYKSEAKVLEAPISFCKRKTGTSKVGFNMSGLREMARIFELLLELKLGRIR
                     GEW"
     gene            794..1243
                     /locus_tag="PLV49_03620"
     CDS             794..1243
                     /locus_tag="PLV49_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOG97965.1"
                     /translation="MHIALRILPASILDNLIAKGRWRFIFKFAFVGATGAVINLTLLW
                     LLTAYVHMYYIISALIAIEISIIWNFFLNTRATFNYNFPEGTAIVRAMARYHAVSFGG
                     MLINLSALLAFTEVAGIHYVASEFLAILIAFTFNYLASINYVWHEAG"
     gene            1464..2873
                     /locus_tag="PLV49_03625"
     CDS             1464..2873
                     /locus_tag="PLV49_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97966.1"
                     /translation="MNAFQSSKENARRINYEERKIKIRILALALLGFLISVKSDRYFP
                     LPDHLPEPVFQLILLPYTCLFFAAFIIFIKPGIVGDAHLSPIDRISAVKLTTVIILIF
                     EAMAFILLIVQDLRYALFGYHLNIPIAIFLFACIGASLIYLLIKDASQGLLVLIALAA
                     YTGTYLLSIASFPLHSQRSDMLPLILSGCQSFLAGMSPYGYHDLLYHPPHQVFTYLPG
                     MWMAYLPAAASGVDLRFINLISMLASALILAYSANGYRNQSTLLIPVFLLTPYLQYRH
                     EIYLGVLFLILSIVFFLDRCNKWWTSSIASGYALATYQFVWVIFPFIVVSAFKRWGIK
                     KAFVNSLIAVAIALILILPFLLGSPEEFIAGVYGNWFYIDTPSINLSYLISLFIPWDS
                     MIIIQGIAMVAIFSLALQRMDPKDCWGWMATALVLFIAMNRVINVYFYLIVLLLLVMH
                     GISVTTPNEKPYGEEEAPN"
     gene            complement(3036..>3451)
                     /locus_tag="PLV49_03630"
     CDS             complement(3036..>3451)
                     /locus_tag="PLV49_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOG97967.1"
                     /translation="SALKDLGRLVAVRGNSDSPRLKRLLPEREVIDVEGVKIGLVHMA
                     SHGSDMVGAEMMAREMNCEVLVFGHIHRPLIEKGRRLLICPGSTAQPRMSAPSVAELM
                     VIDGTVQGRIIPIGSPACNYLKYAEKLGREESEER"
     assembly_gap    3452..3551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3552..3644)
                     /locus_tag="PLV49_03635"
     CDS             complement(<3552..3644)
                     /locus_tag="PLV49_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG97968.1"
                     /translation="MEDDRLPEAVVALASKADIILHAGDFVSVQA"
     gene            complement(3737..4849)
                     /locus_tag="PLV49_03640"
     CDS             complement(3737..4849)
                     /locus_tag="PLV49_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase"
                     /protein_id="HOG97969.1"
                     /translation="MPSVAAFSESMVDFLKPIPENKFVYGLAAGRSALALYSFMQLIQ
                     NHFENVFPFTLEDPVQRHYRKGMNLGIAISGSGETQSVNKYISDFITRGGDIKLITAN
                     KEGTAYKMVSEYEKGDVLVIEARTKHATDKDMRSRLSPLGTEFELEVLAFLNALFADI
                     QCRLKGICDPNCPEYRSIIMDLMENASLISRMDPEHLENWLIRLLPRSGRYIIGGVGR
                     SGLVARAFEMRFTHLNKISYWERDFNTPSFQIGDVYIPITGTGNTYEALEGTTLALKK
                     GADVMPITANPSSRQVSILSKCKRSKDIAVVPVKEEYRSLFSGDTSSTTWLMSDYTKF
                     RYPIFEINASIFTNSVVAVGAEFLGIAEAGMRRMHV"
BASE COUNT         1158 a         1175 c         1140 g         1363 t
ORIGIN      
        1 atgcagaggc atttactgat gtgctgcagg tggaggtgac aaggaaggga tgatgatggg
       61 gtctggtccc ctgtcatccc ggtcttggac cgatccattg gatcttgttt agggtgaaaa
      121 gaactataat gttgacctcg attataattc ccacaaagga tgaaccaacc attcaagaac
      181 ttgtagataa tatcaatgcc ataataaaag aaaaccacga aatactgatt gtagacaaga
      241 gccgcactgc gccaaaggtt aatggggcgg aggttatggt acagagatcg gatggtttgg
      301 gcaacgcttt tgttgaaggc ttgtccagat ccagaggaaa tataatcgct ctcatggatg
      361 gcgacggctc tcaccggcca gaagatctcc agaaaatgct caagctgata gagaatcatg
      421 atatcgtctt gggctcgaag ctcattgagg gtggagagtg cagtgacacc ttgggaagga
      481 tattagtaac cgtcgccttt gctcaactga ccagattgat cctgggcgta gatctgaggg
      541 atcccatgac cggttttatg gtagcaaaga agaatgtatt cgataacctg gagctcaaac
      601 ccaagggatt caagatagtc attgagattg tatataaatc tgaggcgaag gtgctggaag
      661 cccccatatc cttctgcaag aggaaaacgg gcacttcaaa ggtagggttt aatatgagcg
      721 gcctccggga gatggccagg atcttcgagc ttctgctcga gctcaagctg gggcgaataa
      781 ggggcgaatg gtaatgcata tcgccctaag aatccttcca gcaagcattc ttgacaattt
      841 gattgcaaaa ggtcgatgga gatttatttt taagtttgca ttcgttgggg ctacaggggc
      901 ggtaattaat ctgaccttgc tctggcttct gaccgcctat gttcatatgt attatatcat
      961 atctgcattg atagcgatag agataagcat aatatggaac tttttcttga ataccagggc
     1021 gacgttcaat tacaatttcc cagaggggac ggcaattgtt cgtgcaatgg ccaggtatca
     1081 tgctgtctca tttggaggca tgctgattaa cctttccgcg ctgcttgctt ttactgaagt
     1141 tgcaggaatt cactatgttg catccgaatt tctggcaata ttaattgcat ttacatttaa
     1201 ttacctagca agcataaatt acgtatggca cgaggccgga taggacaggc agcagtgcga
     1261 gtatggtcga tatagcgaac tctcctggca agctgatttc aattcaatca tgaaatcatg
     1321 acagagtcga taaaaccagc tctctttttt ccaccatcct taaataatca atcagcgtta
     1381 caaccgcttc gtttaaagca ggctttctgg atttggacag atgccgatcc gatcagcagc
     1441 ctgtgaaggt gacggagacg aagatgaatg cgttccagag tagtaaggaa aacgccagga
     1501 gaataaatta tgaagagcga aaaataaaaa tacggatttt agctctcgca ttgcttggtt
     1561 ttctcataag tgtgaaatcc gaccggtatt ttcccttgcc ggatcatctc ccggagcctg
     1621 tctttcagtt aattctatta ccatatactt gtttattttt tgctgctttt attattttta
     1681 taaagcccgg aatcgttgga gatgctcatc tatcccccat cgaccgcatt tctgccgtca
     1741 agctaacgac ggtcattatc ttgatttttg aagcgatggc atttatcctt ctgatcgtgc
     1801 aagatctcag atatgccctt ttcggctatc atctaaatat cccaattgcc atttttttat
     1861 ttgcatgtat tggagcttcg ctcatttatc tgcttattaa ggatgcatcc cagggccttc
     1921 ttgttcttat cgctctggca gcatatactg gaacatacct tttgagcata gcctcattcc
     1981 cgcttcattc acagaggtca gatatgctcc ccctcatcct ctcggggtgt cagtccttcc
     2041 tggcagggat gagcccatat gggtaccatg accttctcta tcatccaccc catcaggtgt
     2101 tcacttatct gcccggaatg tggatggcct atctgccagc tgctgcttct ggagtggatc
     2161 tgcggttcat aaacctgata agtatgctgg cttctgcgct cattctggca tattcagcta
     2221 atggttacag gaatcagagc acgctgctaa ttccggtatt cctgctgacc ccataccttc
     2281 agtaccggca cgaaatctat ctcggggtgc tatttctcat tctttcaatc gtctttttcc
     2341 tcgataggtg taacaaatgg tggacgagtt cgattgcatc tggatatgct ctcgccacct
     2401 accagttcgt ctgggtcatt tttcctttta ttgtggtttc agcctttaag agatggggaa
     2461 taaaaaaggc ttttgtgaat tcattgatag cagtagcaat tgctttgatc ctcatacttc
     2521 cgttcctttt gggctctccc gaagagttca tcgctggtgt gtacggcaac tggttctata
     2581 ttgacacccc ctccataaac ctctcctatc tgatatctct ttttattccc tgggattcga
     2641 tgataattat ccaaggcatc gctatggtcg ccatcttttc gctggcactg caaagaatgg
     2701 atcctaaaga ttgctggggt tggatggcaa ccgcactggt cttattcatc gccatgaaca
     2761 gggtcattaa tgtgtacttt tatttgatag tccttcttct tttagtgatg catggaattt
     2821 cggtgacaac tcccaatgaa aagccatatg gagaggagga ggcgccaaat tagacagaag
     2881 cattgggccg cattttgctg cagtgagatc tgcctatttt tatagccgct aaacctaaaa
     2941 ccgatgctta ttggagccat catggcgcat cggttttcca cgttgatcaa taatgctaca
     3001 tttttataaa tttttgtttt tacgaagctt gttgctcatc tctcttctga ttcttccctt
     3061 ccaagctttt cagcatactt cagatagttg caggccgggc ttcctatggg gatgatcctt
     3121 ccctgcacag tgccatcaat aaccatcagc tccgccaccg agggagcgga catcctgggc
     3181 tgggcggtgc tcccgggaca gatgaggagc cttctgccct tctctatgag tggacggtga
     3241 atatgcccga ataccagaac ctcgcagttc atctccctgg ccatcatctc cgcccccacc
     3301 atatcagaac catgtgaagc catgtgcacg aggcctatct tcaccccttc cacatctatc
     3361 acctctctct ctggcaagag ccttttgagc cgcggtgagt cggagtttcc cctcaccgcc
     3421 accagccttc ccagatcctt cagagcggaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nggcctggac tgagacaaaa tcgccggcat gaaggatgat gtccgccttg
     3601 cttgctaatg cgaccaccgc ttctggtagc ctgtcatcct ccatcagatg ggtatctgag
     3661 agagctatta tcctggtcat ttcttcatca catcatcctt gaaaaagtgc agccgaggca
     3721 gctattgccc ttgggctcag acgtgcattc gcctcatgcc agcctctgct atgcccagaa
     3781 actccgcgcc cacggccacc acgctgttgg tgaatatgga ggcgttgatc tcaaagatgg
     3841 gatacctgaa tttggtgtaa tcggacatca gccaggtggt cgaggacgtg tcaccgctga
     3901 agagactgcg gtactcctct tttaccggta ctactgctat atccttgctc cttttgcact
     3961 tgctcaggat ggagacctgt ctggatgaag gattggcagt tatgggcatc acatcagcac
     4021 ccttcttcag ggccaacgtc gtcccttcca gggcttcgta ggtgttgcct gttccggtta
     4081 ttggtatgta gacatcgcct atctgaaagc tcggggtatt gaaatccctc tcccaatagc
     4141 tgatcttgtt cagatgggtg aacctcatct caaaggccct tgccaccaat ccggatctgc
     4201 ccactcctcc aattatgtat cttccgctgc ggggaaggag cctgatcaac cagttctcca
     4261 ggtgctcagg atccatgcgg gagatgaggc tggcattctc catcaggtcc atgattatgg
     4321 atcgatactc cggacagtta ggatcgcaga tccccttgag ccggcactga atatccgcga
     4381 aaagggcatt caaaaaggcc aagacctcca gctcgaactc cgtgccaaga ggagagagcc
     4441 tggaacgcat atccttgtcc gtggcatgct tcgtcctggc ttcgattacc agaacgtccc
     4501 ctttctcata ctcgctcacc atcttgtaag cggttccctc tttattggca gtaatgagct
     4561 tgatgtcccc gccgcgagtg ataaagtcgc ttatatattt atttactgac tgcgtctctc
     4621 cagagcctga tatggcaatg ccgaggttca ttcccttgcg gtagtgcctc tgaactgggt
     4681 cctccagggt aaaaggaaaa acgttctcaa aatggttctg gatgagctgc atgaagctgt
     4741 agagggcaag agcggatcgt cccgccgcaa ggccgtagac gaacttgttc tccggaatgg
     4801 gcttgagaaa gtccaccatg gactcggaga acgcagctac gcttggcata tccagatcca
     4861 gcagcttctc caggaagctg cactgtaagt ttgtggattc gcggaaggtt tctatcaggc
     4921 ctgacatagt ggctca
//
LOCUS       DAOSGP010000083         4922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_15285_length_4922_cov_18.092459, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000083 DAOSGP010000000
VERSION     DAOSGP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15285_length_4922_cov_18.092459"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="PLV49_03645"
     CDS             complement(<1..1492)
                     /locus_tag="PLV49_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOG97970.1"
                     /translation="MTLCLFYGASQSRKISRNVFLAWLMMAIGQLCFTLGDGLWAWME
                     TFKNQSLFSSIADIPWLMTYVLFIIGILLLPSIKITPRERTRILLDAAIVIVTSGLFF
                     WSLIFQPAIFQNSNLSPLDMVLIMAYPIFDLILVFLVAEMLFLKLSLPGNEALKFLAL
                     GAGVWIATDTIYFMQILNETYQPGGVLDSGWVAGYLLMGLAGIAQGEAAKRGAFNANQ
                     SIEVRYDRTGWPLYLPYLCAAGAFFMLVWSRNHDFAISFGSLSLSVGVIIGLVFVRQM
                     ILLNENAQLYREAQLEIAERRKAEEEVKRLNEDLEERVRLRTAELEAANKDLLVAKAK
                     AEAAVRAKSNFLANMSHEIRTPMNAIIGMARLLQGTDMKPEQRDFLDTIQKSGGALLA
                     IIDDILDYSKIDREKLELENVPFDLQICIEDSLDLVAIKASEKGLELAYLLENDVPKR
                     IVGDVNRLRQVLVNLLGNAVKFTDRGEVVLKVSSRRLEGNEGKVELH"
     gene            1936..2565
                     /locus_tag="PLV49_03650"
     CDS             1936..2565
                     /locus_tag="PLV49_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97971.1"
                     /translation="MREEFKRMTFEQKVSYLMERENRNKLPDDLAREGVALLAQAGEI
                     EYAAAMARDRGMIDEAISILVDAGDYLWAALIAKNAGRMSQSEMLYQDGMQFYIDMEM
                     FGRAISAATALGMPADRIDDLFRQGVESESRGMDLEHSRGMIESAMESLDISLIGRED
                     EIAVQITKALSEERERRMKEEARALEVLRADNLSADDDLNIDDQEKNGE"
     gene            complement(2610..3527)
                     /locus_tag="PLV49_03655"
     CDS             complement(2610..3527)
                     /locus_tag="PLV49_03655"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOG97972.1"
                     /translation="MLNVTFLGTAGSLPTPERNPSAILINREGEMLLFDCGEGTQRQM
                     MRARTGMMRLNYIFLTHLHADHILGIPGLLETMAFQGRVEPITIAGPIHTARLVECFN
                     SVCYFSRNFEVIALELSPEDVVKMKGYQVRAIETRHSVPSLGYILEEDQRLGRFDRDA
                     ALSLGVPPGPLFGRLQHGHEVAVEERVVQPQQVMGPPRPGRKVVYTGDTRPCRSVEDA
                     SRGADLLIHDSALASDMIEWAMETKHSTAKEAAELARRAGVRHLVLTHISSRYSEDSS
                     QLLNEARLIFEKTDVAEDLMSLEIRFKDE"
     gene            complement(3821..4762)
                     /locus_tag="PLV49_03660"
     CDS             complement(3821..4762)
                     /locus_tag="PLV49_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG97973.1"
                     /translation="MDWLILAFLGTIFFSGAGVLDKLLLKNYTYDSKAYIVCQAFVQL
                     IFIIPVMLFAGVKFTYPESLIALLLGCLQIFPTFFYMKALQIEEASKVSAMEYAYPVF
                     VLIGSIFLFGEILSLRCYAGGLFLIIGTLFLSYKRDRGIGRASIGIYPKIKGSIKGLS
                     PSIRPFMLFWILTAAYYLSLKYLLYSIDEWSLYIWSSLGTMIVVMPLMAVRSIREEVF
                     GFFGRGSFAIGALISEEAFQFLGLIFSIFAYAIGSVTLVSSVGALQPILTVMLIMILG
                     ALAPRLAKELNERTDRSSLMHKGAAFMMVIVGVYFVS"
BASE COUNT         1272 a         1248 c         1203 g         1199 t
ORIGIN      
        1 agtgaagctc aaccttgcct tcattccctt caagccttct ggagctgacc tttagcacca
       61 cttctcccct atctgtgaac ttaacagcat tacccagaag gttgaccagg acttgcctta
      121 agcgattgac gtctcccaca atcctctttg gaacgtcatt ctccagcaga taagccagct
      181 ccagaccctt ctccgatgcc tttatcgcca ccagatccaa agagtcctca atgcagatct
      241 gaaggtcgaa aggcacattc tccagctcca gcttctccct atcgatcttg gagtagtcca
      301 ggatatcgtc gatgatggca agcagggcac cgccgctctt ttgaatggta tcgaggaagt
      361 ctcgctgctc aggcttcata tccgttccct gcaggagacg ggccatgcca attatcgcat
      421 tcatgggcgt tcttatctca tggctcatat tggccagaaa attggatttg gcccttacag
      481 ctgcttccgc cttggccttg gccactaaga gatccttgtt ggcagcctct agctctgcag
      541 tgcgaagcct tactctctcc tccagatcct cgttgagccg cttgacctct tcctccgctt
      601 ttcttctctc tgctatctca agctgagctt cccgatatag ctgggcattt tcgttcagga
      661 gtatcatttg ccggacgaac accaggccta ttatcacccc cacgctgagg gagaggctgc
      721 caaaggatat ggcaaaatca tgattacgac tccagacgag catgaaaaat gcccctgctg
      781 cgcataggta tgggagataa agaggccacc cggtccggtc gtagcgcact tcaatactct
      841 gattggcgtt aaatgcgccc ctcttcgctg cctctccctg ggctattccg gccaggccca
      901 tcaataggta tcctgcaacc caaccgctgt ccaggactcc acccggctga taggtttcat
      961 ttagaatctg cataaaatat atagtatctg tggcgatcca aacacctgcc cccagagcga
     1021 ggaacttcag ggcttcattt ccggggaggc tcagctttag gaatagcatc tctgccacta
     1081 ggaagaccaa gatcagatca aaaatcggat aggccataat cagcaccatg tctaagggac
     1141 tcaaattgct gttctgaaag atggcgggct gaaagataag cgaccagaag aataggccgg
     1201 aggttacgat cacaatggct gcatccagca ggatccttgt cctctctcgc ggggttatct
     1261 taatggaagg caaaagcagt atgcctatta tgaaaaggac ataggtcatg agccatggta
     1321 tatctgcaat tgagctgaac agcgattgat ttttaaacgt ctccatccat gcccataatc
     1381 cgtcccccag ggtgaaacag agctggccga tggccatcat cagccaggcg aggaagacat
     1441 ttcggctgat cttcctggat tgactggccc cataaaaaag acataacgtc atgaaaccgt
     1501 ttatgataaa tgcaatccaa tcagtgaaaa aaatcaccgc atcggtgtca tttctgagct
     1561 gctgaccgat gatcatgtaa attacatata tagcaactgc tataattgtg aagctaaaaa
     1621 cctttgaacc cctctttgga ataatagcag ccctaggaga tacgttctca tgcatgttct
     1681 ctcatcgcca ttcgatcccg ttcagccctt gtgattcctg agggcattta tcatgtagtt
     1741 gcacctatat atctttttgt agatttaaat gcttttgcgt gctactcgct gccgataatt
     1801 tatgaacaca atctcaattt ttgaaatatc tgatgaggag atattatgca attttaaaag
     1861 gtatcttcaa gttgcatata tgctgacaaa aagccttatt tgctggtgca atcatttcat
     1921 ttcggagaaa taagcttgag agaggaattt aagaggatga ccttcgaaca aaaggtctcc
     1981 tatctgatgg aaagagaaaa ccgcaacaaa ttgccggatg acctggcaag agagggcgtg
     2041 gctcttctgg ctcaggcggg tgagatcgag tatgctgcag ccatggcaag ggacagagga
     2101 atgatagatg aggccatcag catcctggtg gacgcaggag attacctctg ggctgccctc
     2161 atagccaaga atgcgggaag aatgagccaa tccgagatgc tatatcagga cggtatgcag
     2221 ttttatatcg acatggagat gtttggaagg gccatctcag ctgcaactgc cctcggcatg
     2281 ccagcagaca ggatcgatga cctcttccgc cagggggtcg agtcggagag caggggcatg
     2341 gacctggaac atagccgggg gatgatcgaa tccgccatgg aatctttgga catctccctc
     2401 atcggcagag aggatgagat agctgtgcag attaccaagg ccctgagcga ggagagagaa
     2461 agacggatga aggaggaagc cagggcattg gaggtactca gagcagacaa cctgagcgct
     2521 gacgacgatc tcaatatcga tgatcaagaa aaaaatgggg aatagacgga ttgatgaatc
     2581 gagagtcatg gaccacaaaa gcgggatctt cactcatcct tgaaccttat ctccagggac
     2641 atcaggtcct ctgctacatc cgttttctca aagatgagcc tcgcctcatt caatagctgg
     2701 gagctgtcct cagagtaacg ggagcttata tgggtcagga ccaggtgtct gaccccggca
     2761 cgcctggcca gctctgctgc ctcttttgcc gtgctgtgtt ttgtctccat cgcccattct
     2821 atcatatcgc tggccagagc agaatcatgg atcaggagat ccgctccccg gctggcatcc
     2881 tccaccgacc ggcagggcct ggtatccccg gtgtatacaa cctttctgcc cggccttggc
     2941 ggccccataa cctgctgagg ctgaaccact ctctcctcca ccgccacctc atggccatgc
     3001 tgcagcctgc caaataaggg ccctggagga acgcccaggg agagagcagc atcacggtcg
     3061 aatctgccga gccgctgatc ttcctccagg atgtagccca ggctgggcac gctgtgacgg
     3121 gtctctatgg ccctcacctg atatcccttc atcttcacca cgtcctccgg cgatagctcc
     3181 agggcgatta cctcgaagtt gcgggagaag tagcataccg agttgaagca ctccaccagc
     3241 cgggcggtat gtatcggtcc ggctatggtt atgggctcaa ctcttccctg aaaggccatg
     3301 gtctccagga gaccgggaat gcccaggata tgatcggcat gaagatgggt taaaaagatg
     3361 tagttcaagc gcatcatgcc ggttctggcg cgcatcatct gcctttgagt tccctctccg
     3421 cagtcgaaaa ggagcatttc cccttcgcgg ttgatcagta tggctgaagg attcctctcc
     3481 ggagtgggta gcgatccggc ggtcccgagg aaggtcacat tgagcataac aggcctctta
     3541 gcgtttctcc cggaatgata acctcacccc atatcatctt ttgcatccaa aacctttatc
     3601 agataaaaga gaaggcactc aaatggtgta ggtatggatg caattactat cgtggagtgg
     3661 cttcaggctt tgttggtact ggttatattc ctggggataa ttgcggtgag cttcaagatc
     3721 tggcgctgga actacgtgat cccacccaag tagggcggga cgtttctaga gcagatcctg
     3781 ccgctgcttt gcagttggac tttttttttc tggcagggat tcagctcacg aagtatactc
     3841 ccactattac catcatgaaa gcggcgccct tatgcatcag cgacgatctg tccgtcctct
     3901 cattcagctc tttggccagc cttggcgcta gggcaccgag gatcatgatc agcatcaccg
     3961 tcaggatggg ctggagggct cctacgctgg ataccagggt gactgatcca atagcatagg
     4021 cgaagatgga gaagatcagc ccgagaaact ggaaggcctc ctctgagatc aatgctccta
     4081 tggcaaagct tccccggcca aagaagccaa aaacctcttc gcgaatggag cgcacggcca
     4141 tgagaggcat taccactatc atggtgccaa gcgatgacca gatgtaaagg ctccactcat
     4201 cgatcgaata caggagatat tttagggaga gataatacgc agcggtaagg atccaaaata
     4261 acataaatgg cctgatcgat ggggataagc ccttgatgct gcctttgatc ttcggataga
     4321 tgccaattga agccctgccg attcctcggt cccttttata agataaaaaa agggtgccga
     4381 ttataagaaa aagaccgcct gcatagcatc ttaggcttaa gatctcgccg aagaggaaga
     4441 tggagccaat taatacgaac acaggatatg catattccat cgctgagacc tttgaagcct
     4501 cttcgatctg cagggctttc atgtaaaaga aggtaggaaa tatctgcaag caaccaagta
     4561 acagagcgat aagggattca gggtatgtga atttgacgcc tgcaaatagc ataaccggga
     4621 tgatgaatat cagctggaca aatgcctggc agactatata ggccttggaa tcatatgtgt
     4681 aatttttcaa cagaagcttg tcgagcactc ctgcgccgga aaaaaatatc gtccccagga
     4741 atgctaatat gagccaatcc attttgaact tccccacagt ttgtgcaagc cgataagctc
     4801 agcagatcgc ttttcgttcc tgtacgcggt ttattttaga ctatttagta atatttatgg
     4861 tactagttat acttatctga aataccaatc caatcgtcaa tctgattaaa cttctgccta
     4921 aa
//
LOCUS       DAOSGP010000084         4823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_15707_length_4823_cov_17.986367, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000084 DAOSGP010000000
VERSION     DAOSGP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15707_length_4823_cov_17.986367"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(549..>2966)
                     /locus_tag="PLV49_03665"
     CDS             complement(549..>2966)
                     /locus_tag="PLV49_03665"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG97974.1"
                     /translation="AGMAAVLVADAVFCIQTQKGTYISGSHLDTIWISCNLFIALAGL
                     LQANPYPFDPLKFLSRRIYSKSPLMAYLPYLGIGATFSLLIWGYEYSQAVNQSIIAAC
                     VGLVMGLMLVRQKIVFDERNQLLTTTLTEIEERKRVEKSLRESEQEKAAILGGLKNVA
                     VEYLDPFMRLIWINDNLQESIGLSADEMLGARCYEAVFGRSEPCSGCTVARAINMGAS
                     QEGDLITPDGRTWISRSSPLKDADGKIIGVVHVLIDISARKSAERALKESERRLADII
                     EFLPDATFVVDKNGKVISWNRAIEKITGVKAELILGKGDYEYSLPFYGERRPTLIDMA
                     KGIDPKIDPEFEKKYENLKRDEENNSLVGEAYMPLQGKGDAYLLGSASALYDSNGDYW
                     GAIESIRDITDRKRVEEELQRSKEMAESATRAKSEFLANMSHEIRTPMNAVIGLTELL
                     MDEKDEKLTSAQRECVEMIRISGDTLLSIINNILDLTKIEANMTELEHRPIDVKGCIE
                     SSMDLVSGSVGDKRIKFSYKIEENVPQFIMGDPTRLCQILINLLNNAVKFTEQGEIAV
                     SVSNTNLDDGGYEIHFAVKDTGIGIPEDKMSRLFRSFSQVDASTARKYGGTGLGLAIS
                     KKLTEMMNGRMWVESAVGVGSVFHFTIRADPIIGEPLDLSKPDNLYAHGKQIKDLERS
                     NSELNILLAEDNVVNQIVTKKMLSKLGYNADVASSGIEALKALEKKKYDLIFMDVQMP
                     EMDGLEAAREIRRRWPEGGPKIIAVTASALKGDQEMCLAAGMDGYIMKPTKIEAIREA
                     LQAAAKG"
     assembly_gap    2967..3066
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3258..3782
                     /locus_tag="PLV49_03670"
     CDS             3258..3782
                     /locus_tag="PLV49_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOG97975.1"
                     /translation="MNIGQKIKLLMADITTLKVDAIVNAANSSLLGGGGVDGAIHSAA
                     GPELLGECRQIGGCPAGEARITRGYRLPARFVIHTVGPVWRGGSEGEDQLLARCYQSC
                     FALAEKNEIKSIAFPAISTGAYGFPIERACRIAIWEMRRNLEDKNAGDTIDVIAVCFN
                     QRTFQCYQGALQEL"
     gene            complement(3849..4682)
                     /gene="arsM"
                     /locus_tag="PLV49_03675"
     CDS             complement(3849..4682)
                     /gene="arsM"
                     /locus_tag="PLV49_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOG97976.1"
                     /translation="MLTLKEREIKRAVKEGYARIAKQATSDYASRCCSGDADIPEELS
                     KRMGYTDEEIQAAPPESNLGLGCGNPVALASLSEGETVLDMGSGAGFDCFLASDRVGP
                     SGKVIGVDITSEMVEKARANSRKGGYANIDFRQGDLENMPVADNYVDVVISNCVINLI
                     PNKRMAFREAFRVLKPGGRLAVSDVVLLKELPEFVKSSTEAYIGCLAGAIMKDDYLDI
                     IKSIGFRDVRVVAESRFPVESLISERSGCSTPKDPAITPEQQKKVADSVLSIKISAIK
                     P"
BASE COUNT         1087 a         1253 c         1123 g         1260 t
ORIGIN      
        1 ctataatgat tatatagata gattgccgtc tcatgaaagg gtctggcggg cgtactgtgg
       61 gccaaaacgc atccggcagt gatctgctga atctggccat tgatttgcct cttcctgcga
      121 gcaaaaattc gtgccgctcc tggagcaggt acattaatcc gtgatgcaga tgatgcatgc
      181 cgtcgaggta attgcattcg gttgcgcgcc tgcgggataa caggcaggat gaaaaagaat
      241 tgcgtcccgt gattggcaca gcaatctgcc tgagatctcg cgccaataca cagtcgggac
      301 atgaggcggc tccatatcaa gatgatgccg aaatcgagat gaagccacac agtgagattc
      361 tgtgggatcc ctgaggggat tgtgcgattc ggtgttgtgc tgcctcgtgt tcggcgccac
      421 agccaagatt caggcggatc ggccaccacc aggataccag gttcctccaa ttgaagtggg
      481 gcgattcatt tccgatccgc ctgaatcttg atcctatgct cagaagatct cactgccatt
      541 ttcactgctc atccttttgc cgcagcttgc agggcttctc taatagcctc tatcttggtg
      601 ggcttcatta tatagccgtc catcccagcc gccagacaca tctcctgatc tcctttaaga
      661 gcagatgcag ttactgctat gatcttcggg cctccctcag gccagcgacg gcgtatctcc
      721 ctcgctgcct ccaggccgtc catctccggc atctgcacgt ccatgaatat caaatcgtat
      781 ttcttcttct ccagcgcctt cagggcctcg atgccgctgg acgctacatc cgcattatag
      841 cccagcttac tgagcatctt ctttgtgact atctgattga ctacgttgtc ctctgctagc
      901 aggatattca attcggaatt agatctctcc agatccttga tctgcttccc gtgtgcgtac
      961 aagttgtccg gctttgacag atctaagggt tcgccgatga taggatccgc ccgaattgta
     1021 aaatgaaata ctgaacccac tccaacagcg ctctcaaccc acattcgtcc attcatcatc
     1081 tctgtgagct tcttgcttat ggcaaggccc aatccagtgc cgccatattt gcgtgccgtc
     1141 gaggcatcaa cctggctgaa agatcgaaat aggcgggaca tcttgtcttc agggattcct
     1201 attccagtat ccttcaccgc aaaatgaatc tcatatccgc catcatccag attggtgttt
     1261 gatacagaga ctgcaatctc gccttgctcg gtgaacttca ctgcattatt gagcaggttt
     1321 atcaggatct gacagagcct tgtgggatct cccatgataa attgggggac gttctcttcg
     1381 attttatatg aaaattttat tcttttatcg ccgacgctcc cggacacgag atccatagag
     1441 gattcaatgc aacccttaac atcgattggc cggtgctcca gctctgtcat gttcgcctcg
     1501 atcttggtga gatcgaggat attattgatg atagagagga gagtatcccc gctgatgcgg
     1561 atcatctcga cgcattccct ttgcgccgaa gtcagcttct catccttctc atccatgagc
     1621 agttctgtaa gaccgataac agcattcatt ggcgtccgga tctcgtggct catgttggcc
     1681 aggaactcgg actttgcccg ggtggctgat tcagccatct ccttggacct ttggagctcc
     1741 tcttccacac gctttcgatc tgttatatca cgtatcgact cgatcgctcc ccaataatct
     1801 ccatttgagt catagagcgc agatgcgctt cccaggaggt atgcatcgcc ttttccctgc
     1861 aggggcatgt aggcctcgcc aaccagcgag ttattctcct catctctctt taggttctca
     1921 tatttctttt caaattccgg atcgatcttt ggatcaattc cttttgccat atctatcagt
     1981 gtaggccttc gttctccgta aaatggcaga gaatattcat agtctccctt gcccaggatc
     2041 aactcggcct taacaccggt tatcttctca atggcgcggt tccaggaaat caccttgcca
     2101 tttttgtcaa ccacaaatgt ggcgtcaggc agaaattcga tgatatccgc cagccgacgc
     2161 tcagactcct tcagagctcg ctctgcactc ttgcgggcgc tgatatctat cagcacatgg
     2221 actaccccga tgatcttgcc atctgcatcc ttgagaggac tgctacgaga gatccaggtc
     2281 ctcccgtccg gagtgatcag atcgccctcc tgggatgcgc ccatattgat agctcgtgcc
     2341 actgtacagc ctgaacaggg ctcactgcga ccgaataccg cctcataaca acgagcgcca
     2401 agcatttcat cagcagagag gccgattgat tcttgcaggt tatcattgat ccagattagt
     2461 cgcataaaag gatcgagata ctcgacagcg acattcttca aaccgcccag aattgccgcc
     2521 ttctcctgct ctgattcgcg caatgacttc tctacgcgct tgcgttcttc aatctcggta
     2581 agcgttgtgg tgagcagttg attcctctca tcgaagacta tcttctggcg aacgagcatc
     2641 agccccatga ccagccctac gcaggctgct attatggatt ggttgactgc ttgggagtat
     2701 tcatatcccc agatgagaag agagaatgtg gcaccaattc ccaggtatgg caggtaggcc
     2761 ataagtgggg atttgctgta gatccttcgg cttaaaaatt ttaatggatc aaagggataa
     2821 ggatttgcct gcagcagtcc tgccaatgct ataaacaggt tgcatgagat ccagatcgtg
     2881 tccagatggc tgcctgagat gtaagtccct ttttgtgtct ggatgcagaa caccgcatct
     2941 gcgaccagta ctgcagccat tccagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnntttg cagatcttat tattttaacc acaatccacc tgatcactat cggcattaat
     3121 gaagctgcta aaggaaaaaa aggttttggt ggaatataat cagtaaacat ttttttcaaa
     3181 agattatgat gcagcgagat gattatggaa aggttaattt ctcttagata tggatagcca
     3241 attggcggat aggaggaatg aatatcggac agaaaataaa actgctcatg gctgatatca
     3301 ccacactgaa ggtcgatgcc attgtcaatg ccgccaacag ctctctgctg ggaggaggtg
     3361 gggttgacgg cgccattcac agtgcagcag gaccggagct tttgggagaa tgcaggcaga
     3421 tcggtggctg cccagcggga gaagcgcgca tcaccagggg ctataggctt cccgctcgct
     3481 ttgtcattca caccgttggg ccggtctgga ggggtggatc agagggcgag gatcagcttc
     3541 tcgccagatg ctatcagagc tgctttgccc tggcagagaa gaacgaaata aagagcatag
     3601 cctttccggc cataagcaca ggggcttatg gctttcccat agagcgggcc tgccgtattg
     3661 ctatctggga gatgaggcgc aatctggagg acaaaaacgc gggtgataca atagatgtga
     3721 ttgccgtctg cttcaaccaa aggacattcc aatgctatca gggggctttg caggagctct
     3781 gattccatcc aatgcaatag tcctcaagat ctctatccaa tgccttgacg cactcattgg
     3841 gtgctgcttt agggcttgat cgcacttatc tttatgctca agacggaatc tgcaaccttc
     3901 ttctgctgct caggagtgat cgcgggatct ttaggtgtgc tgcagccaga tctctcggag
     3961 atcagagact cgacgggaaa tctggattct gccaccaccc gcacatctct gaagcctatg
     4021 ctctttatga tatccaggta atcatccttc atgatggcac ctgccaggca gccgatataa
     4081 gcttctgtgg agctcttgac aaactccggc agctccttga gcagcaccac atcagagact
     4141 gccagccttc ctccgggctt taataccctg aaggcctccc tgaatgccat tctcttattg
     4201 ggaatgaggt tgatcacgca gttggagatg accacatcca cgtagttgtc tgccaccggc
     4261 atattctcaa ggtctccctg ccggaagtcg atgttggcat agccgccctt tcgggagttt
     4321 gccctggcct tttccaccat ctcagaggtt atgtcaaccc caattacctt gcctgatggc
     4381 ccaaccctgt cggatgccag gaagcagtca aatcctgctc ctgagcccat gtccaggacg
     4441 gtctccccct cggataacga tgccagagct actgggttgc cgcagcccag gccgaggttt
     4501 gactccggtg gagcagcttg gatctcctca tcggtgtagc ccatcctctt gctaagctcc
     4561 tctggaatat cagcatcccc tgagcagcac cgggaggcat agtcggatgt tgcctgcttg
     4621 gcaatacgag cgtatccttc tttaactgct ctttttatct ctctctcttt caaagtcaac
     4681 ataaacccca ccgcttctaa gcgttaatcg tgcgctgaca cttttttctg gaaatttatc
     4741 ttgaatgctt agcacaggct ataattaagg ttttttataa cgataattgt cgatgaattg
     4801 caaaagattg agatatcgca atc
//
LOCUS       DAOSGP010000085         4789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_15871_length_4789_cov_20.292564, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000085 DAOSGP010000000
VERSION     DAOSGP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15871_length_4789_cov_20.292564"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLV49_03680"
     CDS             complement(<1..737)
                     /locus_tag="PLV49_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG97977.1"
                     /translation="MLEIKDLSRWLPQEAIELYEGQGYNELYPPQAQAVERGLLEGKN
                     MLLALATASGKTFLAELAMLKAALQSKRSLYIVPLRALAAEKFDSFQRFKDLGVSVGI
                     STGDFDKKDERLGRNQIIIATSEKADSLMRNGASWIRDLAVLVVDEIHLLNDVGRGPT
                     LEMTITKLRRSNPGLQIIGLSATVANSRDLADWLDAELVSSDWRPIALKEGVICRGRL
                     VFSDEERALEAKKDEKKDESIALVRDTL"
     gene            932..2083
                     /locus_tag="PLV49_03685"
     CDS             932..2083
                     /locus_tag="PLV49_03685"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOG97978.1"
                     /translation="MYADRIKNLPPYLFAGIDKAKQEARARGVDVIDLSVGDPDLPTP
                     DHIVQALKQAANDSSNHQYPSYEGKLAFRNAVADWYKKTFDIDLDPKNEVLTLIGSKE
                     GIAHAPLAFINPGDVALVPDPAYPVYATATAFAGGEPAIMPLLRENGFLPDLDAIPAD
                     VARRAKIMFLNYPNNPIGATASEKFFGELVDYARDHNIIIMHDNPYSEIYYDGNRSLS
                     ILEIDGAKDVAVEFHSLSKTYNMTGWRIGSVVGNADVVAGIGKVKSNIDSGTFGAVQD
                     AGIVALGSPKEVVDEIRKVYQQRIEILYKALKDIDLVLEKPRATLYLWAWAGGSSIDY
                     AAKLLERTGIVATPGLGFGKYGEGYMRFSITRETKRVEEAARRLEKMKD"
     gene            2121..>4789
                     /gene="leuS"
                     /locus_tag="PLV49_03690"
     CDS             2121..>4789
                     /gene="leuS"
                     /locus_tag="PLV49_03690"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOG97979.1"
                     /translation="MLESYSAKEVEAKWQRIWEENAVFEPEPDERQKFLLTIPYPYLN
                     GNLHAGHTRTFTIGDAYARYKRMRGYNVLFPMGFHATGTPIVGLSELIANKDPLIWGV
                     YTRLHGIPEEELASLNTPEKIVLYFRRQAKAAMQSIGYSIDWRREFTTTDPAYSKFIE
                     WQYGILRNLDYVVKGSHPVRWCPHDKNPVEDHDILKGEDATIMDFALIKFKMGDTIMP
                     CATLRPETVFGVTNLWLNPSVTYVMARINHESWIVSKAAYEKLTYTDRTVAWEGEIPG
                     IELIGKRVTNPLTGAEVLVLPAGFVDPDNGSGIVMSVPAHAPYDYLALKDLYEEDLSQ
                     YGIKEDLRAIEFISLIESPGYGEFPAVEAVKELGVRDQNDPKAEDATKMVYRREFHNG
                     VLKPNTGCYAGVAVSKIKDLLLRDLIDRGIAEIFYEFSETPVICRCGTRCVIMMVRDQ
                     WFLEYSNPAWKGRVLWCLSRMNIIPPEFRVEFENKIDWLKDKACARRKGLGTHLPWDK
                     EWLIESLGDSTIYMAYYILAKYVNAGMKIDNLPPEFFEYIFMDNGDAAAVADLTGLPG
                     QTVQQIHDDFAYWYPVDLRTSGKDLVANHLLFFLYHHVAIFPEQLWPKAIAVNGFVSL
                     EGQKMSKSKGPLLTLRQAVAENGADVTRLYILGNAEGTADVDWRNDGVEATHAHLDRF
                     YNLAREIMADGSIDESAEKSLIDRWMLSRLQRRIAEATEAMEEIQTRRALQSAFYLLM
                     GDLRWYQRRGGKNQLRAVLSAWVRMMSPFIPHICEELWAAGLGQGYASRAEWPVADES
                     LIDVRAEKAEDLLERTLNDVQEIVNVTKAQPSRIALYTTPQWKKEMLRLAVAAAAGGK
                     LDMGALMKEAMANPAIAEHKKDAPKYAQKLAKAA"
BASE COUNT         1120 a         1266 c         1403 g         1000 t
ORIGIN      
        1 agggtgtctc tgaccagggc gatagactcg tccttcttct catccttctt cgcctcaaga
       61 gccctctctt cgtccgagaa gaccagcctg cccctgcaga tgactccctc cttcagagct
      121 atgggcctcc aatcgctgct caccagctct gcatccagcc agtctgccag gtcacgggag
      181 ttggcgaccg tcgccgaaag gccgatgatc tgcaatccag ggttagagcg gcgcagcttg
      241 gttatggtca tctccagggt aggacccctg ccaacatcgt ttaataggtg gatctcatcg
      301 accaccagga cggccaggtc tcgaatccag gaggcgccgt ttctcatcaa tgagtccgcc
      361 ttctctgagg tggcgatgat gatctgattt ctgcccaggc gctcgtcctt cttatcgaag
      421 tcgcctgtgc ttatgcctac gctcacgccc agatctttga acctctgaaa agaatcgaat
      481 ttttctgccg ccagggcgcg cagagggacg atgtaaaggg agcgcttcga ctgcaaagcg
      541 gccttgagca tagccagctc cgccaggaag gtcttgcccg aggcggtagc cagggcaaga
      601 agcatgtttt tgccttccag aagacctcgt tccacggctt gtgcctgtgg cggataaagc
      661 tcgttatacc cctgcccttc gtagagctcg atggcctcct ggggcagcca gcggctcaaa
      721 tccttaatct ccagcatcaa tctccctttc ggctcgataa cttcaagacg tccgatattc
      781 accgatcttc catatgacct tcaaggatct gcccgggctc ctctaatctt aaatactacg
      841 tgaaagtagt acctgcggga taataacctg gacaggaacg gaaaaagaat taccgtcatc
      901 atataaatgc atcttccatg gagagaacct aatgtatgcc gatcggataa agaatctccc
      961 gccgtacctt tttgccggca tcgataaggc gaaacaggag gcaagggcaa gaggcgtgga
     1021 cgtaatcgat ctgagcgttg gcgatccgga tcttccgacg cccgatcaca tcgttcaggc
     1081 cttaaagcag gcggcgaatg actcatcgaa ccatcagtac ccatcttatg agggaaaact
     1141 cgccttccgg aatgcagtgg cagactggta taaaaagacc tttgatattg atctggatcc
     1201 aaagaacgag gtgctcactc tgatcggctc caaggagggc atcgcccatg caccgctggc
     1261 cttcatcaat ccgggagacg tggcgcttgt ccctgatcct gcttacccgg tttatgcaac
     1321 ggccacggcc tttgccggcg gagagcctgc cataatgccg cttttaaggg agaacggctt
     1381 tttgccggac ctcgatgcca tcccggcaga tgtcgcccga cgggcaaaga tcatgtttct
     1441 caactatcca aacaacccca tcggtgctac ggctagcgag aagttcttcg gagaattggt
     1501 cgactacgct cgggatcata atatcatcat aatgcacgac aatccctact ccgagatcta
     1561 ctacgatggc aatagatccc tgagcattct ggagatagat ggggcaaagg atgttgccgt
     1621 ggagtttcat tccctctcca agacctacaa catgaccggc tggaggatag gatcggtcgt
     1681 gggcaatgcc gatgtcgtgg cgggaattgg caaggtcaag agtaacatcg attcgggaac
     1741 ctttggcgca gtgcaggatg caggaatcgt ggccttgggc agcccaaaag aagtggtgga
     1801 tgagatccgc aaggtctacc agcagcgcat agagatcctg tacaaggccc tcaaggatat
     1861 cgatctagta ctggagaagc ccagggcgac gctataccta tgggcctggg caggtggaag
     1921 ctccatcgat tatgctgcaa agctgctgga gaggacggga atcgtggcca cgcctggtct
     1981 cggatttgga aagtacggcg agggctacat gcgcttttcc atcaccagag agacgaagag
     2041 ggtagaagag gcagcaaggc gcctggagaa gatgaaggat taggtggatt cgggccacag
     2101 aaagtctgga gggaaccata ttgcttgaaa gttattccgc aaaagaagtc gaggccaagt
     2161 ggcagaggat ctgggaggag aatgcagtct ttgagccgga gcctgatgag cgccagaagt
     2221 ttctcttgac cataccctat ccttatttga atggtaatct gcatgccggt cacacccgca
     2281 cattcaccat aggcgatgca tatgctcgct acaagaggat gaggggctac aatgtcttat
     2341 tccctatggg gtttcatgcc acaggaacgc ccatcgtggg cctgtccgag ctcatagcca
     2401 ataaggatcc tctcatctgg ggggtctaca ctcggctgca tggtattcct gaggaggagc
     2461 tggccagcct gaatacgccg gagaagatag tgctctactt ccgacgccag gccaaagcag
     2521 caatgcagtc catcggctac tccatagact ggaggcggga gttcaccacc actgatcccg
     2581 cttacagcaa gttcatcgaa tggcagtacg gcatactgcg aaatctcgat tatgtggtca
     2641 aaggcagcca tccagtgcgc tggtgtccgc acgacaagaa tccggtggag gaccatgaca
     2701 tcctcaaggg cgaggatgcc accatcatgg acttcgccct gatcaagttc aagatggggg
     2761 atacgattat gccctgtgcc acacttaggc cggagaccgt cttcggtgtg accaacctct
     2821 ggctgaaccc aagtgtcact tatgtcatgg cgaggatcaa ccacgagagc tggatcgtct
     2881 caaaagcagc ctatgagaag ctgacctata ccgacagaac tgtggcctgg gagggggaga
     2941 tacccggaat tgagcttatc ggcaagaggg tgacaaatcc actcaccggg gcagaggtgc
     3001 tcgttctgcc cgcagggttc gtggatccgg ataacggctc agggattgtg atgtccgttc
     3061 ctgcccatgc cccttacgat tatctggccc ttaaggatct gtatgaggaa gacctgagcc
     3121 agtatggcat caaagaggac ctgagagcaa tcgagttcat atccctgata gagtcgcctg
     3181 gctacgggga gttcccggca gtggaagcag tgaaagagct cggggtccgg gatcagaatg
     3241 accccaaagc ggaggatgcg accaagatgg tctaccgccg cgagtttcat aatggagttc
     3301 tcaagcccaa caccggctgc tacgcgggcg tggcggtgag caagatcaag gacctgctgc
     3361 tcagggatct gatcgatcga gggatagccg agatcttcta tgagttctcc gagaccccgg
     3421 tgatctgtcg ctgcggcacc aggtgcgtta tcatgatggt gcgggaccag tggttcctgg
     3481 agtactccaa tcccgcctgg aagggcaggg ttttatggtg cctgtccagg atgaacataa
     3541 taccaccgga gttccgggtg gagttcgaga acaagatcga ctggttgaag gacaaggcct
     3601 gcgccaggag gaagggcctg ggaactcatc tcccctggga taaggaatgg ctgattgagt
     3661 ccctgggaga ctcgaccatt tacatggcct actacatcct ggccaagtat gtgaatgcgg
     3721 ggatgaagat cgacaacctg cccccggagt ttttcgagta catcttcatg gacaacggtg
     3781 atgccgcagc tgtagcggat ctgaccggcc tccctggtca gacggtgcag cagattcacg
     3841 acgacttcgc ctactggtat cccgtagatc tgcgcacatc gggcaaggat ctggtggcca
     3901 accacctgct cttcttcctc taccatcatg tggccatatt cccggagcag ctctggccga
     3961 aggctatagc cgtgaacggc tttgtctcgc tggaggggca gaagatgtcc aagtccaagg
     4021 gaccgctcct cacgctccgc caggcggtgg cggagaacgg ggcggatgtg acaaggctct
     4081 acattctggg caatgccgag gggacggcgg atgtggactg gagaaatgat ggggttgagg
     4141 ccacccatgc tcatctggac cggttctaca atctggcgcg ggagataatg gccgatggga
     4201 gcatagacga gagtgcggaa aagtcgctca tcgaccgctg gatgctctcc cggctgcaga
     4261 ggcgtatagc tgaggccacg gaggctatgg aggagattca gacccgcagg gctttgcaga
     4321 gtgcctttta cctcctcatg ggagacctgc gctggtatca gcggcgggga gggaaaaacc
     4381 agctccgtgc agtgctctct gcctgggtga ggatgatgtc gcctttcatc cctcatatct
     4441 gcgaggagct ttgggcagca ggccttggcc agggatatgc ctctagagca gagtggccgg
     4501 tggcagatga gtctttgatc gatgtgcggg cggagaaggc agaggatctc ctggagagga
     4561 cgctaaacga tgtgcaggag atcgtcaatg tcaccaaagc ccagcccagc aggatcgccc
     4621 tttacaccac accccagtgg aagaaggaga tgctgcgcct tgccgtggcc gctgcagcgg
     4681 gaggaaagct ggacatgggt gcgctgatga aggaggcaat ggccaatccg gcgatagccg
     4741 agcacaagaa ggatgctccc aagtatgccc aaaagctggc caaggcggc
//
LOCUS       DAOSGP010000086         4778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_15929_length_4778_cov_10.467288, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000086 DAOSGP010000000
VERSION     DAOSGP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15929_length_4778_cov_10.467288"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3338)
                     /gene="cobN"
                     /locus_tag="PLV49_03695"
     CDS             complement(<1..3338)
                     /gene="cobN"
                     /locus_tag="PLV49_03695"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HOG97980.1"
                     /translation="MNESTVGDAYRRIFQYISFGGRENLRNLLLFLANLLGDAALPVI
                     DPKMPPWEGIYHPQFDHVPSLQEYLDMKLIPGRLTVGLWFNISMWQAEDLEAIDLIIL
                     EIEAKGANVIPVFLHTRKDEQLGSGGWERAAKDYFMNDGHPIIDVLISTLMFSLTIGP
                     VARNLPGGSDSDSSMRDSGTRERLDGASSTEKAAAEGASALQEMGVPVIKALLNYYST
                     KEEWSASFQGLGPMDVTINVAQPEFDGMLISVPVGFRDPSGRDPLTGASLSRFKPMEE
                     RIQKIVRLALNWARLRHTAPEDRKAAIIFHNYPPRDDQIGTAIGLDSPESVYRILHGM
                     AKEGYRLDWLPDSGQDLMNRILKGATNQKKFYSMQCLLERADALVSGEQYRAWFEELP
                     ALVQEQMTNAWGMPPGDLFVHNDFMIIPGIVNGNIFISMQPPRGLLENPEAIYHSPDL
                     PFPHHYYAYYRWIRDIFGAHVVMHIGKHGSLEWLPGKSVGLSESCYPDIAISDLPNIY
                     PYIINNPAEGTQAKRRSYCCLVDHLVPVMRNADAYEEMAELEVLIKEYRHASIEDRKK
                     LADLKERIWQKTVEANLDSDLGLTEYPEGRQFDEFVEKLCDYIYELSDSQVRNGLHIL
                     GLPPSGIKQDEFLVSLTRLKNGSVPSLREAVSNLMGYDYDQLLDGRARTVDGKKTGGE
                     ILQEIHHHSLRLIEELHKSNFSTGDLQGVQLEILGRKSREVESVLRYICEFLVPRLEE
                     TVQEIDSILDAARGGYVMPGPSGAPTRGMADILPTGRNFYSLDPRAIPTRAAWKVGVS
                     QAESLLERYIQDEGNYPENIGIILWSTNTMRTCGDEIAEILYLMGVRPVWEERSGRID
                     GLELIPLQELGRPRIDVTVRISGIFRDAFPNIVELMDEAVQMVADLKESPEENYLAHH
                     VELRMKEKMDAGNCEETAREEAGYRIFGSRPGTYGAGVSAAIESKNWKDCHDLAEVYV
                     VWGGYAYGRRSFGVTAAEEFKRRLCSLDLTVKNEDTREIDMLDGDDFYSFHGGMISAI
                     KSIKGELPRSYCGDSSDPDRVKTRSAAEETKHIFRTRILNPKWIKSMQEHGYKGAADF
                     ARAVDISFGWDATAEVLEDWMYEEL"
     gene            complement(3641..4657)
                     /locus_tag="PLV49_03700"
     CDS             complement(3641..4657)
                     /locus_tag="PLV49_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG97981.1"
                     /translation="MIREIIIALIICFCLLIPVCSGDTISVVDYTEKQVNLSLPVTSI
                     ISLSNMATEIICALDGGNSLIGRPNSVFPTYIDKVEIVGENSRSPDLERIVQLHPDLL
                     IADGMLSDDNRKEIEDAGIPVIIEKFTDPARVIIITENMGQILGKEERAGEIVDFVNE
                     YEKLIDSRTAAAKPDEETSAYVEWNSAYRAASTSSGYNNFIEKASGSNIVTNSSVQYP
                     TIDPEFLIQQDPSVIVKMLSSTKEYREEDLIKEHDEISSRSELSSLKAVEEGRIYILS
                     GIVAGGMRSVIGELYFAKWLHPELFQDIDPEAIHEEMAERFFGQELENAYAYPSLKMD
                     LSRK"
BASE COUNT         1115 a         1314 c         1079 g         1270 t
ORIGIN      
        1 agctcctcgt acatccagtc ctcaagaacc tcggcagtgg catcccagcc gaaggatata
       61 tctacggccc tggcaaagtc ggctgcgcct ttgtagccgt gctcctgcat acttttgatc
      121 cacttgggat taagaatgcg cgtccgaaaa atatgcttcg tctcttcagc agcgcttctg
      181 gtcttcaccc tgtcaggatc ggagctgtcg ccgcaataag atctaggcag ctcacctttg
      241 atggatttta tagcggagat catgcctccg tgaaacgaat aaaaatcgtc gccgtccagc
      301 atatcgatct ctctcgtgtc ctcgttcttt actgtcaggt ccaggctgca caagcgcctc
      361 ttgaactcct cagcagctgt aaccccaaat gacctgcgac cataagcata tcctccccag
      421 accacataga cctctgccag atcgtggcag tccttccagt tcttggactc gatggcagcg
      481 ctcactcccg cgccataggt tccgggccga cttccgaaga tgcgatagcc cgcctcctcc
      541 ctggccgtct cttcacaatt gccggcatcc atcttctcct tcatcctgag ctccacgtga
      601 tgagccagat agttctcctc tggggattct ttgaggtccg cgaccatctg taccgcctcg
      661 tccatcagct ccacgatatt cggaaaagca tccctgaaga ttccgcttat gcgcaccgtg
      721 acatcgatgc gaggccttcc cagctcctgc agcgggatca gttccagccc atcgattcgg
      781 ccgcttcgct cctcccaaac ggggcggact cccattaaat agagtatctc ggcaatttca
      841 tcgccacagg ttcgcatggt gttggtgctc cagagaatta tgccaatatt ctccggatag
      901 ttaccttcat cctgaatata ccgctccagt agcgactcag cctgcgatac gcccaccttc
      961 caggccgccc tggtgggtat ggctctcgga tccaggctgt agaagtttcg cccagttggc
     1021 aagatatcgg ccatgcctct tgtgggagcg ccggaaggac ctggcattac atagccacct
     1081 ctcgccgcat cgaggatgct atcaatctcc tgtacggtct cctccaatct tggaacaagg
     1141 aactcgcaga tatagcgaag gactgattca acctcgcggc tctttcttcc caagatttca
     1201 agctgcaccc cctggagatc gccagttgaa aaattgctct tgtgaagctc ctcgatcagc
     1261 cgaagagaat gatgatgaat ctcctgaaga atctccccac ccgtcttttt gccatcgaca
     1321 gtccttgccc tgccgtccag gagctggtcg taatcgtatc ccatcaagtt tgagacggcc
     1381 tctctcaagg atggaacgct cccgttcttg agcctggtaa gggataccag aaattcatct
     1441 tgcttgatcc ccgaaggcgg caatcccagg atatgcaaac cgtttcggac ctgagaatcg
     1501 gacagctcat aaatatagtc gcatagcttc tctacaaact catcgaattg acggccttct
     1561 gggtactccg tgaggcccaa gtcactatcg aggttggcct ctactgtctt ctgccagatt
     1621 cgctccttta agtcagccag cttttttcga tcctcaatag atgcatgccg gtattctttt
     1681 atgagaacct ccagctctgc catctcctcg tatgcgtcgg cattgcgcat tacaggaacc
     1741 agatgatcga ccagacagca atagctgcgc cttttggcct gggtgccttc ggctgggttg
     1801 ttgatgatgt agggatagat gttgggaaga tcggatatag cgatatccgg atagcagctc
     1861 tccgataggc ccactgactt tcccggcagc cattccagcg atccgtgctt tccaatatgc
     1921 atgaccacgt gagcgccgaa gatgtccctg atccagcgat aataggcata gtagtgatga
     1981 ggaaatggca gatccgggct gtgatagatc gcttcgggat tttccaacaa gcctctgggc
     2041 ggctgcatgc ttatgaagat attaccattg acaatgccgg gaatgatcat gaagtcgttg
     2101 tggacgaaca gatctcctgg cggcattccc caggcgttcg tcatctgctc ctggaccaaa
     2161 gcaggcagct cttcgaacca tgccctgtac tgctcgccgg agaccagagc gtccgccctc
     2221 tcaaggaggc actgcatgct gtaaaacttc ttttgattgg tagcaccctt caggatccta
     2281 ttcatcagat cctggccgct gtcaggcagc cagtcgagcc tgtatccttc cttggccatc
     2341 ccgtgcagga ttcgataaac cgactccgga gaatcaaggc cgatggcggt tccaatctga
     2401 tcgtcccttg gaggatagtt atgaaagatg attgccgcct tcctgtcctc tggggctgta
     2461 tgtcgcaggc gcgcccagtt gagagccaga cgcacgatct tttgtattct ctcttccatg
     2521 ggcttgaatc gggaaaggct ggctccggtc agtggatctc tgccggacgg atcccggaat
     2581 ccgacaggca cacttatcag cattccgtcg aactctggct gggccacatt aatagtaacg
     2641 tccatcggcc caagcccctg gaatgaggca gaccactcct cttttgtgct gtaataattt
     2701 agcagggcct ttataaccgg aactcccatc tcctgaagag ccgatgcccc ttcagctgct
     2761 gccttctctg ttgaactggc tccatccagc cgttccctgg ttccagagtc cctcatagag
     2821 gagtcgctat cgctccctcc tggcaagttt cttgcgaccg gtccgatcgt gagagagaac
     2881 atcagtgtgc tgatcaatac atcaattatg ggatggccat cgttcataaa atagtctttt
     2941 gcggcccttt cccaaccgcc gcttccaagc tgctcatcct tgcgggtatg caggaacacc
     3001 ggaatgacat ttgcgccttt tgcttcaatc tccaggataa tcaaatcgat ggcttccaga
     3061 tcctcagctt gccacatgct gatattaaac caaaggccca cagtgagcct tcctggaata
     3121 agcttcatgt ccagatactc ctgcagagat ggtacgtgat cgaattgcgg atggtagatc
     3181 ccctcccagg gaggcatttt tggatcgatc accggaaggg ccgcatcacc aagcaggtta
     3241 gccagaaaaa gcagaaggtt cctgaggttc tctcttcctc caaagctgat gtactgaaaa
     3301 attctcctgt acgcatctcc taccgttgat tcgttcacaa actcaggatc aatacagctt
     3361 gcatcctgaa cgtgaagaag ggccccagac tccctgatgg cagaaattag tcgatcatat
     3421 ccaggaaggc tcttttttcc gccatgcagc ctgattatga ccaaatcgga ttttatagca
     3481 atgtctttta cttcattcgt ccgaattaag gattcgcctg caattggatg caatgctgca
     3541 aaatcgccca gctcttcttt caattctctg aaggccgagg ccagcggcga aatatccgaa
     3601 ccgctaatgc taatatatgt gatctttgtg gtattcgacc tcattttcgg ctcaagtcca
     3661 tttttaatga tggataggca taggcgtttt caagctcctg gccaaaaaat ctttcagcca
     3721 tctcctcatg aatcgcctcc ggatcaatat cctggaaaag ctcaggatgg agccactttg
     3781 cgaaataaag ctctccgata acagagcgca ttccgccagc aacaattccg cttaaaatat
     3841 atattcttcc ttcctcgacg gccttcagag aactcagctc ggatctgctg gagatctcat
     3901 cgtgttcctt gatcaaatcc tcttcgcggt attccttggt gcttgaaagc attttgacga
     3961 ttacagaagg atcttgctgt attaggaatt ccggatcaat tgtcgggtat tgtactgatg
     4021 aattggtaac gatgttggat ccgctggcct tttcgatgaa attattatag ccgctgctcg
     4081 ttgatgctgc acggtatgct gagttccact cgacatatgc gcttgtctcc tcatccggtt
     4141 tggcagctgc agttcgacta tcaattaact tttcatactc attgacaaag tcaacgatct
     4201 ctccagcccg ctcctctttt cccaggattt gtcccatatt ttctgttatg atgatcacgc
     4261 gagccggatc ggtgaatttt tcaataatca caggaattcc tgcatcctct atctctttgc
     4321 gattgtcatc ggagagcatc ccgtctgcta tcaagaggtc gggatgaagc tgcacgatgc
     4381 gctccaaatc tggcgacctg gagttctccc ctacaatctc cactttatcg atatatgtgg
     4441 gaaaaactga attcggccgg ccaattagac tatttccgcc atccagagca cagattatct
     4501 ctgttgccat attggacagg gaaattatgc tagttacagg taatgataga ttcacctgct
     4561 tttcggtata gtcaacgaca ctgatagtat ccccgctgca tacaggaatc aataggcaaa
     4621 aacagattat taacgcgatg attatttctc ttatcattgt aacatcctca agacatattt
     4681 tttggattta agacaaaatc acttgtattt aagacctccg gagtaagaag taattgacga
     4741 aattatataa gataaattta ttcattaaaa tttaaaaa
//
LOCUS       DAOSGP010000087         4739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_16123_length_4739_cov_21.992741, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000087 DAOSGP010000000
VERSION     DAOSGP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16123_length_4739_cov_21.992741"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLV49_03705"
     CDS             complement(<1..281)
                     /locus_tag="PLV49_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOG97982.1"
                     /translation="MEIAFVGGSESVLGFNLAGVKKSYAANTEDEMVSKIGDVMADSN
                     VGILVLKQSDYNKLPKRLQEQLSESIKPTMITIGTEQSTEMREKIKRAIG"
     gene            complement(283..1332)
                     /locus_tag="PLV49_03710"
     CDS             complement(283..1332)
                     /locus_tag="PLV49_03710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HOG97983.1"
                     /translation="MPVLRLPKFGLPVKYAYITGRVRAMKTKLIPAEMYPRMMNMEMS
                     EIARYLEETQYKEEIDALAKDYSGVDLIEHATFDNMAKTFRKLAEVSIDEPSFLILEY
                     LRRWDVWNIKTLLRGKFSGASDTEIMKYLVPAGELSPEHLEELAKKDSIQDIISAFDG
                     TDYASALAQYDGKNLASLENALDKLYYFRMERAVGGTLSVGGGLLLKYVRREIDIKNL
                     ITLFRMNKAGIDAAVIQENLIPGGKLHDELSRMAGQPFGEFLRGLEGYPFWSSISDIA
                     TSDLDAVSRVEARLKAYLIRYAWALSNYHPLSILPVLGYMVSKETEVSNIRKIVRGKE
                     AGLPSELIEEQVVVA"
     gene            complement(1338..1886)
                     /locus_tag="PLV49_03715"
     CDS             complement(1338..1886)
                     /locus_tag="PLV49_03715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOG97984.1"
                     /translation="MALDAVVESVLATSKDKVAQITSEADLEAARILNEARERAAEIK
                     SRKESEVGHAIEAIERREISSANLEVKRSELNVHKDLLEKARTKLLEKISNLPKKDNE
                     AMLKKMLEPYDLKDMKVYSNKRDEAFLSSLAPNYGGNLDIIGGVVVESGDGALRYDLS
                     YETLAREVFNNRMKEVSKVLFG"
     gene            complement(1915..2181)
                     /locus_tag="PLV49_03720"
     CDS             complement(1915..2181)
                     /locus_tag="PLV49_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOG97985.1"
                     /translation="MVVVADAGILYGLLAVGAGLATGLAGIGAGVGEQGIGAAVVGVV
                     AEEPGFLGKGLFLMLLPETLIIFGLAVSLILMFAWTPFSAIVTP"
     gene            complement(2249..4282)
                     /locus_tag="PLV49_03725"
     CDS             complement(2249..4282)
                     /locus_tag="PLV49_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOG97986.1"
                     /translation="MLRPVNMSRVVVAGSKDVISPVVEKLHELRLLHIVNYNGNQPEF
                     ALGKPIGKAREYSENLIKLRSVTRYLDIKNKAPEKTYPESQVLSEMDNLLNNVGGDVT
                     ATYERIVAIEAEIKSKQDQIRALTPLSILPLPLEAYHGYDSLATFVGTAVTPVDADVA
                     RVSPVNEIFTGTAKNATYVAVFVPAEKASEVSAVLSEHGFSEMSVPKLEGSVDSAIAS
                     LNGSIKSLEGELVPLQKKLVDVKKQYEDLMLATDEYLSMQVSKTEAPLRFAETDNAFV
                     IDGWVPSDQYSRLKSELESAAGGKLDVQLIADKEADDLVKGGEDVPTKMDNPGIVKPY
                     ELITRLFAIPEYKEFDPSLLIFVFFPIMFGMILGDVGYGIVILLILTLLKKRFRTEGW
                     QQLINIVMIAGVWAVIFGIFYGEIFGPLGLWNYMVGMLSHHEMIELQEMGLFFGEGTF
                     GPLGRLGPLGIFPLYRLATNAVLLLIGVSLFIGVIHCSVGSILGIKTELNYGEKRHAY
                     FERLPVMLFQVFFAIALLGLVMGQMIMVYIGAAVIVLSIVMMVMGPEGAMGLAHVPSY
                     VSNLISYLRLLAIGLASVGLAYAANQLSFYVIMPMLSGGATNSADFSILAIIVGIVIL
                     VAVHFINILLGILSPFMHPLRLHYVEMFTKFYSAHGGGVEYSPFGHVRRFLKA"
     gene            complement(4275..4601)
                     /gene="ahaH"
                     /locus_tag="PLV49_03730"
     CDS             complement(4275..4601)
                     /gene="ahaH"
                     /locus_tag="PLV49_03730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase archaeal subunit H"
                     /protein_id="HOG97987.1"
                     /translation="MARDEILSKIKSAEAQSRAAVQQALEEKEKKIAAATTEAMNLVK
                     SAEIDAQDYYDKSVAKAESDVKAKKQSIIQSGDKNVASMSGSASAKLDKAVEYLLKEF
                     MGSLHA"
BASE COUNT         1136 a         1322 c         1048 g         1233 t
ORIGIN      
        1 cctattgccc ttttaatctt ctctctcatc tctgtgctct gctcggttcc gatggtaatc
       61 atggtaggct tgatagactc ggataactgc tcctgaagcc tcttgggcag cttattataa
      121 tccgattgct tcaacaccag aattccaaca ttggaatcgg ccatgacatc gccaatcttt
      181 gagaccatct catcttccgt gttggcagca tatgacttct tgactcctgc cagattgaac
      241 ccgaggacac tctcgcttcc acctacaaaa gcaatctcca ttttaggcca ccaccacttg
      301 ttcctctatg agttcagagg ggagtccagc ttccttgccc ctaacgatct ttctgatgtt
      361 ggaaacctct gtctctttgc ttaccatata tccgagcacc ggcaggatgg atagcggatg
      421 ataattggaa agagcccatg cataccgtat gagataagcc tttaacctgg cttcgactct
      481 gctaacggcg tcaagatctg atgttgcgat atcggatatt gacgaccaaa atgggtatcc
      541 ctccaatccg cgcagaaact cgccaaaagg ctggcctgcc atgcgggaca gctcatcgtg
      601 cagctttcct ccaggaataa ggttctcctg tattacggct gcatcgatgc ctgctttgtt
      661 catccggaag agagtgatca gattcttgat gtctatctcc ctacgaacgt atttgagcag
      721 cagtcctcct cctactgata atgtgccgcc tacagcgcgt tccatcctga agtaatagag
      781 cttgtccagt gcattctcca gagatgcaag attcttgcca tcgtattgtg ccagagcgct
      841 ggcataatcg gtgccatcaa aggcggagat gatatcctga atgctatcct tcttcgccag
      901 ctcctcaagg tgctctgggg atagctcgcc cgcaggcacg agatatttca tgatctctgt
      961 gtcggaggct cccgagaact ttcctcgcag gagtgtcttg atgttccaga catcccagcg
     1021 cctcagatac tccagaatca ggaatgatgg ctcgtctata gagacttccg ccagcttcct
     1081 gaaggtcttc gccatattgt cgaatgtggc gtgttctatc agatccacac cggagtaatc
     1141 cttggcgagc gcgtcgattt cctctttgta ctgggtttct tccaggtatc gtgcgatctc
     1201 tgacatctcc atgttcatca tgcggggata catctccgca ggaatgagct tggtcttcat
     1261 cgctctgact ctgcccgtga tgtaagcgta cttcaccggt aaaccgaact tcggcaaacg
     1321 tagtacaggc atcgtcctca cccgaatagt accttggaaa cctctttcat gcggttatta
     1381 aagacctcac gcgcaagagt ttcatatgac aaatcgtaac gtagagctcc atcaccgctc
     1441 tcaaccacaa cgccgccgat gatatccagg ttcccgccgt aattaggggc gagagaggat
     1501 aggaaggcct catctcgctt gttggagtag accttcatat ccttgagatc gtatggctcg
     1561 agcatcttct taagcatggc ctcattatcc ttcttgggaa gattggatat cttctcaagg
     1621 agcttcgtcc ttgctttctc caggagatcc ttgtgaacat taagctccga tctcttgacc
     1681 tcgagattgg cgctggagat ctccctgcgc tcgatagcct ctatggcgtg tccgacctca
     1741 gactccttcc tggatttgat ttcagcggca cgttccctag cttcattgag tattcttgca
     1801 gcctcaaggt ccgcttcgct cgttatctga gcgaccttgt ccttactggt tgccagaacc
     1861 gactcaacca ctgcatctag tgccattgcc tctcaccctt tggaaatggc cagctcaggg
     1921 tgtcactatt gcagagaagg gcgtccaggc gaacatcagg atcagcgata cagcgaggcc
     1981 gaagatgatc agggtctcag gcagaagcat gaggaaaaga cccttaccca ggaatccagg
     2041 ctcctcagcc actacaccaa ccacagccgc tccgataccc tgctcaccaa cacctgcacc
     2101 gatgcctgct aatccagtag cgaggccagc gccaacagcc aacaatccat acagtattcc
     2161 cgcatctgct acaactacca ttttcctcaa tcctcctttt tatgaatatt acaatctgtg
     2221 ctcaaatttt taatctcaaa gatcgctttc atgccttcaa gaacctccgg acatgtccga
     2281 aggggctata ctccactcca ccaccatgtg cgctgtagaa tttggtgaac atctccacat
     2341 aatgcaacct gagaggatgc atgaagggag agagaattcc cagcagaatg ttgatgaaat
     2401 gcactgctac cagtatcact atgcctacga tgatcgcaag tatagagaaa tcggcactgt
     2461 tcgtcgctcc accgctcaac atgggcatta tgacatagaa cgacagctgg ttggcagcgt
     2521 atgccagacc taccgaagcc aggccaatgg ccagaagcct aaggtaggat atcaggttgc
     2581 tcacatagga tggcacgtgt gccagaccca tggcgccttc cggacccatg accatcatca
     2641 ctatggataa tacgatcact gcggcaccga tgtacaccat gatcatctgt cccatgacca
     2701 atccaagaag cgctatggcg aagaacacct ggaagagcat cacaggaagc cgctcgaaat
     2761 aggcatgcct tttctcgcca tagttcaact ccgtcttgat tcccagaatg gagcccacag
     2821 aacaatggat cacgccgatg aacaggctaa cgcctatcaa aagcagaaca gcattcgttg
     2881 ccaatctata taacgggaag atccccaacg ggccgagcct tcccagcggg ccgaaagttc
     2941 cttcgccgaa gaacaagccc atctcctgaa gctcaatcat ctcatgatga gatagcattc
     3001 ctaccatata gttccacagg ccaaggggcc cgaagatctc accgtagaat atgccgaata
     3061 tgactgccca tactcccgca atcatgacga tgttgatcag ctgctgccac ccttctgtcc
     3121 tgaatctctt ctttaacaga gtcagaatca gcaggatcac aatgccatag cctacgtctc
     3181 ccaggatcat gccgaacatg atcgggaaga agacgaatat aagcagcgat ggatcgaact
     3241 ccttgtactc cggaatggcg aatagcctgg tgatcaactc atatggtttg acaatgcctg
     3301 gattatccat cttggttgga acgtcttcgc cgcctttgac aaggtcatca gcctctttgt
     3361 cggcaatcaa ttggacgtcc agctttcctc cagcagcact ctccaactcg ctcttcagcc
     3421 ggctgtactg atcgctgggg acccagccgt ctatcacaaa ggcgttgtcc gtctctgcga
     3481 acctgagagg agcctcagtc ttgctgacct gcatcgacag atactcatcc gtcgccagca
     3541 tcagatcttc atattgcttc ttaacatcaa caagcttctt ttggagaggc actagctcgc
     3601 cttccagaga ttttatgctg ccattgagtg aagcaatagc tgaatcaaca ctgccttcga
     3661 gcttgggaac ggacatttca gagaagccgt gctcggaaag gaccgcagat acctcgctgg
     3721 ccttttctgc aggcacaaat actgccacat aagtcgcatt cttggcggtg ccggtaaaga
     3781 tctcatttac tggagagacc cgggctacat ctgcatccac aggcgtaaca gcagtgccta
     3841 cgaatgtcgc cagagaatca taaccatgat aagcttccag gggcaacggc aatatggata
     3901 acggagttaa agccctgatc tgatcctgct tggacttgat ctctgcttcg atagcaacga
     3961 tcctctcgta ggtggcagtg acatcgcccc ccacgttatt gagaagattg tccatctcgg
     4021 atagaacctg tgactcagga tatgtcttct caggcgcctt gttctttata tcaaggtacc
     4081 tggtgaccga tctgagctta atgaggttct cagagtactc cctggctttg cctatgggct
     4141 ttcccaatgc aaattcaggc tggttgccat tgtaattcac aatgtgcaat aagcgcaact
     4201 catgcagctt ttctactact ggagatatta cgtcctttga cccggctacg accacacgac
     4261 tcatattaac gggcctaagc atgtaacgac cccataaact cctttagcaa atattcaact
     4321 gctttgtcca gcttggcact ggcgctgccg ctcattgagg cgacgttttt atcgccactc
     4381 tggatgattg actgcttctt tgcctttaca tcgctctctg ctttggcaac gctcttgtca
     4441 tagtagtcct gtgcatcaat ctcggcggat ttgacgaggt tcattgcttc ggtagtggca
     4501 gcggctattt ttttctcctt ctcctccaga gcctgctgga ctgcagccct ggattgggcc
     4561 tctgccgatt tgattttcga taagatttcg tctctagcca tctgatccct ctacatattc
     4621 cctcactaca ccactcctgt ttttcaaaca gcgctacatc acttttggct cgtgataaac
     4681 gctcttttgc atcctcatct accaatataa actcttcggt tttggtttga agagaggag
//
LOCUS       DAOSGP010000088         4717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_16214_length_4717_cov_18.613256, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000088 DAOSGP010000000
VERSION     DAOSGP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16214_length_4717_cov_18.613256"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3671)
                     /gene="cobN"
                     /locus_tag="PLV49_03735"
     CDS             complement(<1..3671)
                     /gene="cobN"
                     /locus_tag="PLV49_03735"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HOG97988.1"
                     /translation="MKLVSITWSSELPHLMQGARELSFNLEAWSYTQLDDPVQLEKCL
                     KSLKSAQMVLIHPSNDPCWDEIIPSLSPSTPVISFGRDPSLWTVANVPMDTTLTVNRY
                     ALFGGRKNFKNLLKYACNQALKTSFQLEPPEEILWQGLYHPRSETAFATVDEYLEWYQ
                     GKERSWVGLIFSRTSWANEDLKVVDAAILELEKEFDVLPAFCFGMGDSDLGAWSSVEV
                     AKRFFSGRVEAIVNLQPIFRSRNSDEALQTMKALNVPIFHPAVVYHKTEEEWKDDVHG
                     LSSSEVGWSVAMPEFEGVIEPLMIGATSRSEMDGAQIEKHVPIPERVSKLARRVKKWV
                     ALKNKPVSERRVAFILHNNPCASVEASVGGGAHIDTLESVARIMDRMVQAGYSLQSPP
                     KTGKELIETIMARKAISEFRWTSIEEIIKRGGALALLSEEEYRQWFDLLSPVVRDQMI
                     EAWGRPPGEEKDGIPAAMLYQGKIVITGVEYGNALVCTQPKRGCAGSRCDGQVCKILH
                     DPQVPPTHQYLATYRYIESCFGADLIVHVGTHGNLEFLPGKSVGLSADCYPDIAIGDM
                     PHLYIYNSDNPPEGTIAKRRSYATLIDHMQVVMTDSELYGELKELEDRISEYRRAVLS
                     EGGRAHALEHIILNLLEKSNLAGEINLKRLIATGASFEEILERAHDKISQLYNTQIPD
                     GMHIFGELPAGKRREEMIRAILRAEVRGAAALLLGREVTANIEDLRELDGLGEDLVRA
                     IIADWTGKIEGQDKSSISRLLEERSIGKGSPALDLLRDRIEELSWRIEESREMESLLR
                     GFSAGFIEPGPSGLMSKGKIEILPTGRNFFSLDPSAVPTEAAWMVGRRLADSLLEKYE
                     KEQGRLPENVGMFWMAGDVMYADGEQMAQMFHLIGVMPVWKGGKLKGYEVIPIEELGR
                     PRIDLTVRVSGITRDCFYNCIEIMDQAICEVAALDEPQDMNFLHKHDLENGSHHRIFA
                     SRPGTYGNGVNLAVYASAWKADKDLSDVFIYWNGYAYGKGIFGEESQDGLIKQLKSVD
                     VTFNKTTTDEKDLFGCCCYFGNQGGMTAAAREVSGHHVEAYYGDTRDADRVEVRTLSD
                     EVRRVVRTKLLNPKWIAGMKRHGYKGAGDISKRVGRVYGWEATTQEVDDWIFDDIART
                     FVMDEENRKFFQENNPWAMEEMSRRLLEAEQRGLWKADPEVLDALKNLYLEIEGWIEE
                     RMGDVEGEIQGGSIDVFNA"
     gene            complement(3815..>4717)
                     /locus_tag="PLV49_03740"
     CDS             complement(3815..>4717)
                     /locus_tag="PLV49_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG97989.1"
                     /translation="KVVGISDTVKNKGYYFPDLKGKQSVGKWNALDYEMIGAIASDGE
                     DSIVPDIVVIGYSYPDKSYGIYGLEEGLLPFENIKAVALEFTKPENMTREIEVLGQIL
                     GKEEQASDYIRWYDEKINSVKDAVDGMNLPEVYVEWSSSGGDLSTLGPGSAFDQVLKV
                     AKGYNIASGLEESYPKVDWEWVVTENPEVIIVRQTQPAGHTEIGWESAPSQEASKMQA
                     AKNALLDRSGASGTSAAKSDRIYFIDWDIMNGLDQVVGATYLAKVLHPDVDLNPEEVY
                     SEYLERLGLEYPEGRTMVYPEIED"
BASE COUNT          921 a         1450 c         1176 g         1170 t
ORIGIN      
        1 tcggcattga agacatcgat cgatccaccc tggatctcgc cctccacgtc tcccattctc
       61 tcctctatcc agccctcgat ctccaggtaa aggttcttga gggcatccaa aacctcggga
      121 tctgccttcc acagtcccct ctgctcggcc tccagcagcc tcctgctcat ctcttccatc
      181 gcccaggggt tgttctcctg gaagaacttg cggttctcct cgtccatgac gaaggtccgg
      241 gcaatgtcgt cgaatatcca gtcatccacc tcttgtgtcg tcgcctccca tccatagacc
      301 cggcccacgc gcttggagat gtcccctgct cccttatagc cgtgcctttt catccccgct
      361 atccacttgg gattgagcag tttggtgcga acgacccgcc tgacctcatc gctcagggtg
      421 cgaacctcca cccgatcggc atctctggta tcaccgtagt aggcctcaac atggtgcccc
      481 gagacctccc gggcggcagc agtcatacct ccctggtttc caaagtaaca gcagcagccg
      541 aacaggtcct tctcatccgt ggtggtcttg ttgaaggtga cgtccacgct cttcagctgc
      601 ttgatcaaac catcctggga ttcctcgcca aagatgccct tgccgtaagc gtaaccattc
      661 cagtagatga aaacgtccga gagatcctta tccgccttcc aggcgctggc atagactgcc
      721 aggttcactc cattgccata ggttcctggg cggctggcga agatgcgatg atgagatccg
      781 ttctccaggt catgcttatg cagaaagttc atatcttgtg gctcgtccag tgctgcgacc
      841 tcacagatgg cctggtccat gatctcaatg cagttgtaaa agcagtctcg ggtgatgcct
      901 gagacccgca cggtcaggtc tatcctgggc cggcccagct cctcgatagg tatcacttca
      961 tagcccttca gcttcccccc cttccagacc ggcatgacgc cgatgagatg gaacatctgg
     1021 gccatctgct ccccatcggc atacatcaca tctcctgcca tccagaacat ccccacgttc
     1081 tcaggaagcc gtccctgctc tttttcgtac ttttccagca ggctgtcggc cagtcttcgt
     1141 cccaccatcc aggcggcctc ggtgggaact gccgaggggt ccagggagaa gaagttcctc
     1201 cccgtgggca agatctcgat ctttcccttg ctcatcagcc cggatggtcc tggctcgatg
     1261 aatcctgcgg aaaagcccct caataggctt tccatctctc tggactcctc gatcctccag
     1321 ctgagctctt ctattcgatc tcgcaagagg tctaaggcag ggcttccctt tccaatagag
     1381 cgctcctcca gcagtctcga tatgctgctc ttatcctggc cttcgatctt gcctgtccag
     1441 tcggcaatga ttgcccggac cagatcctca cccagtccgt ccaactctct aagatcctcg
     1501 atattggccg tgacctcccg tccaagcagg agggcagcag ctcccctcac ctcagcccgg
     1561 agaatggccc ggatcatctc ctctcttctc ttgcccgcgg gaagctctcc gaatatatgc
     1621 atgccgtcgg ggatctgggt attgtagagc tgggagatct tatcgtgtgc cctctccagg
     1681 atctcttcga agctcgcgcc ggtggcgatg agcctcttca ggttgatctc gcctgccagg
     1741 ttgctcttct ccagcagatt gaggatgata tgctcgaggg catgggctct tccgccctcc
     1801 gagaggaccg cccgtctgta ctcggagatc ctgtcctcca gctctttaag ctctccatag
     1861 agttcggagt cggtcatcac cacctgcatg tggtcgatta aagtggcata gctccttctc
     1921 ttggctatgg tcccttccgg gggattatct gagttgtata tgtaaaggtg cggcatatcg
     1981 ccgattgcaa tatcgggata gcagtcggcg gagaggccca ccgactttcc gggcagaaac
     2041 tccaggttgc catgggttcc tacatgcaca atcaggtccg cgccaaagca gctctcgata
     2101 tagcggtagg tggccagata ctgatgagtg ggcggcacct ggggatcgtg cagtatcttg
     2161 cagacctggc catcgcagcg agacccggcg caccccctct tgggctgggt gcagaccagg
     2221 gcatttccgt attccacccc agtgataacg atcttcccct gatagagcat ggcagcggga
     2281 attccgtcct tctcttcccc tggcggcctt ccccaggcct cgatcatctg atcccggacg
     2341 acgggggata gcaggtcgaa ccattgccgg tattcctctt cgctcaagag ggcgagggct
     2401 cctcctcgct tgatgatctc ctcgatcgag gtccagcgaa actccgatat cgcctttctg
     2461 gccatgatgg tctcaatcag ctcctttccc gtcttgggtg ggctctgcag cgagtatccg
     2521 gcctggacca tccggtccat gatgcgagca acgctctcca gggtatcgat atgtgctccg
     2581 ccaccaactg aagcctccac tgaagcacag ggattgttgt gcaggatgaa agccacccgg
     2641 cgctcggata ccggcttgtt cttcagggcg acccattttt tgacccttct cgcgagcttt
     2701 gaaaccctct ccgggatggg cacatgcttc tctatctgtg ccccgtccat ctcagagcgg
     2761 ctcgtcgctc ctatcatcag aggctctatc actccttcaa actccggcat ggccacagac
     2821 cagcccacct cgctgctgga caggccgtgc acatcatcct tccattcctc ctctgtctta
     2881 tgatagacga ccgcaggatg gaatatgggc acattcaggg ctttcatggt ctggagtgcc
     2941 tcatcgctgt ttctggagcg gaagatgggc tggaggttga ctatagcctc cactcttcct
     3001 gagaagaacc tcttggccac ctctacgctg gaccaggcac caagatcgga atctcccatg
     3061 ccgaaacaga atgcagggag gacgtcgaac tccttttcca gctccagtat ggcggcatct
     3121 acgaccttca gatcctcgtt ggcccaggag gttcgggaga agatcaggcc cacccacgat
     3181 cgttccttgc cctgatacca ttccaggtac tcatcaacgg ttgcgaaggc tgtctcagat
     3241 cggggatgat agagcccctg ccagaggatc tcctctggcg gttcgagctg gaagctcgtc
     3301 ttcagggcct ggttgcaggc atacttgagc aggttcttga agttcttccg cccgccgaac
     3361 agggcatagc ggttgacggt taaggtagta tccatcggga cattggccac tgtccagagt
     3421 gatgggtccc ggccaaagga gatcactggc gtgctggggc tgagggaggg aatgatctca
     3481 tcccagcagg gatcgttcga tggatggatc aggaccatct gagcgctttt cagtgatttc
     3541 aggcatttct ccagttggac gggatcgtca agctgggtat aggaccaggc ctccaggtta
     3601 aaggagagct ctctggcccc ctgcatcagg tggggcagct cactgctcca ggttatagag
     3661 actaacttca ttcgattacc tgccgattga gggattgcaa tttggtgtga tgtattctgt
     3721 ttttcttgta ttctattaat aactatttcg gaccaaataa cataaaagcc tatctaaaaa
     3781 agaataaaga tgcctcacag gcttattgtt gcgatcaatc ctcaatctcc ggatagacca
     3841 tcgtccttcc ctcgggatac tcaaggccca gacgctctaa atattcgctg tatacctcct
     3901 ccggattgag atccacgtcg gggtggagca ccttcgccag ataggtcgcg cccaccacct
     3961 ggtccaggcc gttcatgata tcccaatcta tgaaatagat ccggtcggac tttgcggctg
     4021 aggttccgct tgctcctgat ctgtccagaa gcgcattctt cgcagcctgc atcttagacg
     4081 cctcctgtga gggagcagac tcccagccga tctcggtatg tcctgctggt tgggtctgtc
     4141 tgacgatgat cacctcagga ttttctgtta ccacccattc ccagtccacc tttggatagc
     4201 tctcttccag gccactggcg atgttgtagc cctttgctac tttcagcacc tggtcaaatg
     4261 cagatcctgg acccagagtg gaaaggtcgc cgccactgga gctccactcc acatatacct
     4321 cgggcaagtt cattccgtcc actgcatcct tgacggagtt gattttttca tcataccagc
     4381 gaatataatc ggaagcttgc tcctccttgc caaggatctg gcctaaaacc tcgatctcgc
     4441 gggtcatgtt ctccggcttg gtgaactcca gcgctaccgc cttgatgttc tcgaagggca
     4501 gaagtccctc ctccaggccg tatatgccgt agctcttatc tggatagctg tagccaatga
     4561 ccacgatatc tggcacaatg ctatcctctc catcgctggc gatcgcgccg atcatctcat
     4621 agtccagggc attccatttc cctacactct gctttccctt gagatcgggg aagtagtagc
     4681 ccttattctt cactgtatct gagattccta ccacctt
//
LOCUS       DAOSGP010000089         4714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_16228_length_4714_cov_21.258425, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000089 DAOSGP010000000
VERSION     DAOSGP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16228_length_4714_cov_21.258425"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLV49_03745"
     CDS             complement(<1..293)
                     /locus_tag="PLV49_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97990.1"
                     /translation="MEGNELRYSILAAMLILMASSAMAFGGPGSVVVDIVGSSVEDTT
                     VVADGRGDIDLEIIGSTAKNTIVSPFKATSYRSCQICPQCPTYPTPIYPTPECP"
     gene            complement(412..831)
                     /locus_tag="PLV49_03750"
     CDS             complement(412..831)
                     /locus_tag="PLV49_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97991.1"
                     /translation="MRKQLILTAVFILSISWAQGISDGRTASLDIVGSNVNNTTFISL
                     DGVPVALDIIGSTTSNIQVGALAKDLQSQCRYCYECGACGEPKIYTTPWDDFRRPLCY
                     PWSSYIPTRYNKPMINWNTGSMAQEPVSLGQGTGVSG"
     gene            933..1835
                     /locus_tag="PLV49_03755"
     CDS             933..1835
                     /locus_tag="PLV49_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HOG97992.1"
                     /translation="MKLIYVAGKGGVGKSVCSAATGIWASEKGLETLVFSMDPAHSLS
                     DIFDIDLGSQPNKIKEHLFAYEPDLAEESRSFFRRYKNMITALFGLFEVEVKPEDFAS
                     MPGVSELIFMDKLNDIYVQKKYDLVVIDSAPTAMVLPLLQLPSITTGFVTKVLGIRNK
                     WIGVLNMLESGFGDSILKEVRAMRLKAETMRNALTDPKTASITVVTIPEKAAVEESRR
                     LIETVQSHGVNVSAIVINHVIGECPCQFCQEKMASQTSYIQDIRNRYHDKDIVILPDY
                     GGEVKGDRLNRVAEDLYVKGQMEI"
     gene            complement(1913..4093)
                     /locus_tag="PLV49_03760"
     CDS             complement(1913..4093)
                     /locus_tag="PLV49_03760"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOG97993.1"
                     /translation="MWKYILLMLIAVSVGLMSPAGADGIIIVDPPPGVDVRLDQSLAI
                     KYHHVDIQIKDQVATTRVDQVFVNDNPWTAEGTYIFPLPEGAAVSDFVMWVDGKAVHG
                     EILEADEARTIYDDVVRRMKDPALLEYVGRKALKASVFPIPPGEERKIELEYSQILPV
                     ENGLVHYIYPLSTERFSSRPLEDLVVRAQIESREPLKAVYSSRHEVSIDREDDYHALL
                     GLEQSDVLPDRDFELFYTISSEKIGLNLLSFKEEGQDGFFLLLAAPDVKVNEEEIVVK
                     DIILVLDTSGSMQGEKMDQAKEAARYVLDHLNPLDRFAIVSFATTTRSFSPSLEPAAQ
                     ADKGKDFLDRLEAMGSTDINRAMIEAVGLAEEVRPTTLIFLTDGLPTEGVTVTGAILD
                     NVARESPDNVRIFSFGVGDDVDTDLLDQISLDHGGASTYVRPGEEIDEEVSAFYRKVK
                     MPVLSDLSLDWGDIIVDQVYPQRIPDLFAGSQLILLGRYREGGPAKITLKGMVNQEER
                     SYTYEDLSFRKEGGDDFIPRLWATRAVGYYLTQIRLYGEKQEWIDSIVSLSTRYGIIT
                     PYTSFLVQEKDIFSDKGREEVISDFKEEMAAAAAEPAFGEAAVEKVVYQKSLSAAPVG
                     AAVPVNMSVSTGIDGTSKMVRVSEVLKNVGSKTFLLKNDTWIDTTFDRSMKTKKVAFL
                     GEEYFDLISQVPVLGSYFALGERVIVVHEGQAYETVAEDDSGSG"
     gene            4298..>4714
                     /locus_tag="PLV49_03765"
     CDS             4298..>4714
                     /locus_tag="PLV49_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97994.1"
                     /translation="MFEKVLIPTDFSRYTSKMAECVTEIPGTREVVLLNVLDASNPKL
                     LEKSGWSYDAIISEAAFRLNEEAKLLASITCKNEVSLMVKPILKVIVEPMSGADGINL
                     NPPEPRQDADFVYGGTVGDAIQKTAWEENVSLIIMGA"
BASE COUNT         1100 a         1269 c         1212 g         1133 t
ORIGIN      
        1 ggacattcag gtgttggata tatgggtgtg ggatatgtcg gacactgcgg acagatctgg
       61 catgatcgat aacttgttgc cttaaatggg gaaacgatgg tgtttttcgc tgtgcttccg
      121 atgatttcca ggtctatatc tccacggcca tcggccacaa ccgtcgtatc ctccacactg
      181 ctccccacaa tatccacgac cactgatcct gggccgccaa aggccatggc gcttgatgcc
      241 atcagaatca gcattgctgc cagtatagaa tacctcaact cattcccctc cattggacat
      301 aatatttaat ggatgtttta tatatactga aagtagaaaa gcttttcggc tggcggaaga
      361 gaccactaac agcctgccag ccagagaggc tttttccatc tttctctgtt atcagcccga
      421 gacgccagtt ccctggccca gagagactgg ctcctgtgcc atgcttcccg tattccagtt
      481 gatcatgggc ttattgtagc gggtgggaat ataggaactc caaggatagc ataaaggccg
      541 cctgaagtcg tcccatggcg tggtatagat cttcggctca ccgcatgcgc cgcattcata
      601 gcaatagcgg cactgtgatt gtaaatcttt agccagagcg cctacctgga tattactggt
      661 ggtgcttcct attatatcca gcgcaaccgg aacgccatcc agcgatatga aagtggtgtt
      721 attcacattg ctcccaacga tatccaggct cgctgtcctc ccgtcgctga taccttgcgc
      781 ccagctaatg gacagaatga acactgcagt taatattagt tgcttcctca tcctttcgcc
      841 cccttttata attaatttca ttaaaatgtc ttgaaagtag aaaaaccttt ttattagcaa
      901 acaattaaat ccacccccga tcagtacagg ccatgaagct catttatgta gcaggcaagg
      961 gcggcgtggg aaagagcgta tgttctgcag cgacaggcat atgggcctct gaaaagggct
     1021 tagagaccct tgtcttctcc atggatcccg ctcattctct ctctgatatc ttcgatatag
     1081 acctgggaag ccagccgaat aagatcaaag agcatctatt tgcatatgag ccagaccttg
     1141 ccgaggagtc acgcagcttc tttcgcagat ataagaacat gatcactgcg cttttcggcc
     1201 tcttcgaggt ggaggtgaag ccggaggact ttgccagcat gcctggcgtc tccgagctga
     1261 tattcatgga caagctaaat gacatctacg tccagaagaa gtacgatctg gtggtcatag
     1321 actctgcgcc cacggccatg gtcctccctc tgctccagct gccctccata accaccggat
     1381 ttgtcaccaa ggtgctgggg atcagaaaca aatggattgg ggttctcaat atgcttgaat
     1441 cgggatttgg ggattccata ctaaaagagg tgagagccat gcggctgaag gctgagacca
     1501 tgagaaatgc gcttaccgat cccaagaccg cgagcatcac cgttgtgacc atcccggaga
     1561 aggcggcagt ggaggagagc agacggctga ttgagacggt acagtcgcat ggagtgaacg
     1621 tctccgccat tgtcataaac catgtgattg gagaatgccc ctgccaattc tgccaggaga
     1681 agatggcctc tcagacctcc tacatccagg atatccggaa tcgctaccat gataaggata
     1741 ttgtaattct gcccgactac ggcggagagg tgaagggtga taggctgaac cgggtggcag
     1801 aggacctcta tgtaaagggg cagatggaga tataaaggaa aaaattggaa aaatccgggc
     1861 cgggcgagca gatatctccc gaccctcctg ttcgctcatt tttcttgttg ctctaaccag
     1921 aacccgagtc gtcttcggcc accgtctcat aggcctggcc ctcatgcacc actatcaccc
     1981 tctcacccag ggcgaagtag cttcccagaa ccggcacttg ggagatcaga tcgaagtact
     2041 cctcgcccag gaatgccacc ttcttcgtct tcatggagcg gtcaaatgtg gtgtctatcc
     2101 aggtgtcatt tttcaagagg aaggtcttgc tgcccacatt cttcaacacc tctgagaccc
     2161 gcaccatctt actggttcca tcaatcccag tagagacgga catattaact ggcaccgctg
     2221 cccccactgg tgcggcgctc aatgacttct gatagacgac tttctctaca gctgcttccc
     2281 caaatgcagg ctcagctgct gccgcagcca tttcctcctt gaagtctgaa attacctcct
     2341 ctctaccctt gtcgctgaag atgtccttct cctgcaccag gaaggaggtg taaggagtga
     2401 ttatgccgta gcgggtgcta agggagacga tggagtctat ccactcctgc ttctccccgt
     2461 aaagccggat ctgagtgaga tagtatccta ccgcccgggt ggcccagaga cggggtatga
     2521 agtcgtcgcc gccctctttc cggaagctga gatcctcata ggtatagctt ctctcttcct
     2581 ggttgaccat tcccttcagg gtgattttcg ccggtccgcc ttctctgtac cttccaagca
     2641 ggatgagctg agagccggcg aaaaggtccg ggatcctctg gggatagacc tgatcgacta
     2701 tgatatcgcc ccagtctaag gagaggtcgg agaggacggg catcttcacc ttgcggtaga
     2761 aggcgctgac ctcctcatcg atctcttccc ctggacgtac ataggtgctc gcaccgccgt
     2821 ggtccagaga gatctggtcc agcaggtcgg tgtccacatc gtcgcccacc ccaaagctga
     2881 agatgcgcac attatccggt gactctcgcg ctacattgtc aagtattgct cctgtcaccg
     2941 tcaccccctc ggtgggcagg ccatcggtga gaaagatgag ggtggtgggg cggacttctt
     3001 ccgccaggcc cacagcctcg atcatcgccc ggttgatatc cgtcgatccc atggcctcca
     3061 ggcggtctaa gaaatccttt cccttatctg cctgggcggc gggctcgaga ctggggctga
     3121 aagatctggt ggtagtggca aaggagacga tagcaaaccg atccagagga ttgaggtgat
     3181 ccaggacata tctggcagcc tccttggcct ggtccatctt ctcgccttgc atgctgcccg
     3241 aggtgtccag gactagaatt atatccttga ccactatctc ctcttcgttt acctttacat
     3301 ccggtgctgc caggaggagg aagaaaccgt cctggccctc ctctttaaag ctgagcaggt
     3361 tcaggccgat cttctcgctg ctgatggtgt agaagagctc gaagtccctg tccggcagga
     3421 catccgattg ctccagtccc agaagggcat ggtagtcatc ctccctatct atggagacct
     3481 catggcgaga ggaatagacg gccttcagcg gttctcgcga ctcgatctgg gccctgacga
     3541 ccagatcttc cagcggccgg gaggagaaac gttctgtgct caagggatag atgtaatgaa
     3601 ccaggccgtt ttccaccggc aggatctggc tgtattccag ctcaatcttg cgctcctctc
     3661 ctggggggat gggaaacact gaggccttga gggcctttct gcccacatac tcgagcagtg
     3721 ctgggtcttt catcctccgc accacatcgt catatattgt ccgggcctca tcggcttcca
     3781 ggatctcgcc gtgcactgcc tttccatcca cccacatcac aaaatcgctc accgcagcgc
     3841 cctctggcag gggaaatatg taggttccct cagcagtcca tggattgtca ttgacgaaga
     3901 cctgatccac ccgggtggtg gccacctggt cctttatctg gatgtccaca tgatggtact
     3961 tgatggccag agactggtcc agacgcacgt ccactccagg tggcggatcg acgatgatga
     4021 tcccatccgc tcctgcgggg ctcatcaggc ccacagatac ggcaattagc attagcaaga
     4081 tgtatttcca cataatccca cactcgatat cagcccaaaa gagccgatcg atcttaacaa
     4141 attataatgc gtcattggta taaatagatc ttatcctcat cttggccctt gccatcgtcg
     4201 tttgattgat tcaagacata tcgccatttt gatatatttt gacggagaca gattcatgaa
     4261 caattcctga gtagagaata gcgaggggtg agaggaaatg tttgaaaaag ttctgattcc
     4321 gaccgacttc tcccggtaca ccagcaagat ggccgaatgt gtaacggaga tcccagggac
     4381 cagagaggta gtgcttctca atgtcctgga tgccagcaat cccaagctcc tggagaagag
     4441 cggatggagc tatgacgcca tcataagcga ggcggctttt cggctcaatg aagaggcaaa
     4501 gctgctcgct agtattacct gcaaaaatga ggtttctctc atggtgaagc ccatcctgaa
     4561 ggtgatagtg gagcccatga gtggggcgga tgggataaat ctcaatcctc cggagcccag
     4621 gcaggatgcg gattttgttt acggaggaac tgtgggcgat gccattcaga agacggcatg
     4681 ggaggagaat gtatccctga taatcatggg agca
//
LOCUS       DAOSGP010000090         4658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_16482_length_4658_cov_19.320878, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000090 DAOSGP010000000
VERSION     DAOSGP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16482_length_4658_cov_19.320878"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1150)
                     /locus_tag="PLV49_03770"
     CDS             complement(98..1150)
                     /locus_tag="PLV49_03770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG97995.1"
                     /translation="MKIIQVSPYFPPHLGGVEYHVKELADGLAKRGHQVTVASSCGRW
                     NNGFVRIPSIDLFYVPIPIKRPNLEADIYHSHVPSPLFAFVLRDSSPHVVTYHNDVVI
                     PGNLNGHYLPRPVGAAVEGVNRKIIRPILDRAKIVISTTKSYAETSPILKDYIHKTKI
                     VPNAVDVSLYPRKREKKGYVLYAGRLLHYKGIESLIQAMSEVQKKADLELVLVGDGYD
                     RSRLEEMARRLDVRARFTGRLDRSRFIDTLSHAEVLVLPTQNRLEAFGIVLLEAMACE
                     TPVLAFKTPGVAEVAGEGGMIYSSPGELGEMILELHESPNLRMNLGRKGRMAVEEKYS
                     WPRALDMMESVYREVA"
     gene            complement(1203..>2516)
                     /locus_tag="PLV49_03775"
     CDS             complement(1203..>2516)
                     /locus_tag="PLV49_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG97996.1"
                     /translation="DPDGDRILYRFLLNGEEMKNWSRSGSWSFLTHYLPAGEYIITVL
                     ARDGLHSSSDSFDSSLNATMVILEQNQPPILKGLESDPESPSPQGSQITWTATAIDPE
                     QDEISYRFLLDGQDMTGWMPSNSWVWNSSGVVAGDHKITVQIMDGPHASQNSIDLEMN
                     RPYTLNESTIGESSAESSVAPLAISPEDPSKPADVPASASSTASLNQIPVFAELKPDA
                     ISPLEIGAIINWTAKAADPDGDELSYKFLLDGQDMTGWSSSPSWTWDTSDALPGTHRI
                     TVLARDGKHAPTDSFDDSIDAEFTLMDKNLPPVLTSLVPDLSSPRVLGETIVWKAEAM
                     DPDNDPILYKFQLGGKDMIRWSESNSWSWSTRGLAADDYQITVLVRDGRHASEYSFDN
                     SLAESFRLKTSIDQQIDLLMSQRGFNASGDTEYSSSDIMLKVADT"
     assembly_gap    2517..2616
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2617..3728)
                     /locus_tag="PLV49_03780"
     CDS             complement(<2617..3728)
                     /locus_tag="PLV49_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HOG97997.1"
                     /translation="MRTARQIPWYLIASILISLSAIALANLFAPPLKEAGSYLALALV
                     ILIPGYLAVISLFPAAGDLDLNRRFLLSLGASLLLAGLISLILYFTPRGLQAASLATI
                     LSFLVLFLAALSYLRWSALPRNKRFVIGAKRSYRSRRTSGRISSGFVSSRGPLLIALA
                     AILVLAGLALAFYHYQPGMNFLSSPKGYTDLEVTWPKSELDGSSEGQYTTLTAGRDLE
                     ARARIDNHEGIPVNYSLRLAFDNSTIFVKGLRLADNETWESMLGFVLEGQPGRQRLSL
                     LLFKEGDSTTPYKSEHLLVDLVDDQSEDQEEMENSTNESMASSDGLPVSFEEKTKVTV
                     LSAGGGGSSVAQVSASAASSSSSAKSKPQSAATETE"
     gene            complement(3803..4567)
                     /gene="xth"
                     /locus_tag="PLV49_03785"
     CDS             complement(3803..4567)
                     /gene="xth"
                     /locus_tag="PLV49_03785"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOG97998.1"
                     /translation="MRILSWNVNGLRAIYKKGFVDWLLKDRPDVLCLQEIKATEDQIP
                     KELKGLPGYISFFSPGARKGRDGVALFTKTKPLSLEYSLSFVGFDDEQRAIVADYGDF
                     LLFNVYFPNGKASKERLSYKMRFYDLFLDLMDRLVAEGRDIVICGDVNTAHKEIDLAR
                     PKPNEKISGFLPEERAWIDRLIEHGFLDTFRLFHPEGEKYSFWDMKTRARERNVGWRI
                     DYFFVSLGMRDRVKSAFILDDVYGSDHCPVGIEIEL"
BASE COUNT         1075 a         1196 c         1226 g         1061 t
ORIGIN      
        1 ggcgaacaat gtggccttca atctctcatt catcaattgg aaggtagatc tattctgtaa
       61 atacttattg actcgaagat ctcccaagac actactctca ggccacctct ctgtagaccg
      121 actccatcat atccagggcg cgaggccagg agtacttctc ttccaccgcc attctgccct
      181 tccggccgag gttcatcctc aggttcgggc tctcatgcag ctcgagtatc atctcgccca
      241 gctccccggg gctggaatag atcattcccc cctctcctgc cacctcagcc accccaggag
      301 ttttgaaagc caggacggga gtctcgcagg ccatggcctc gagaaggacg atgccaaatg
      361 cctccaggcg gttctgggtg gggaggacca gaacctcggc atgagagagg gtatcgatga
      421 accgggaccg atccagcctg ccggtgaacc tggccctgac atcaagcctt ctggccatct
      481 cctccaggcg gcttcggtcg tagccatccc ctacaaggac cagctcgagg tcggccttct
      541 tctgcacctc gctcatggcc tggattaggg actcgattcc tttgtagtgc agcagcctgc
      601 cagcatagag aacatagccc ttcttctctc tcttccgggg ataaagggat acgtccactg
      661 cattgggcac tatcttcgtc ttatgaatgt agtccttcaa gatgggagag gtttctgcat
      721 agctttttgt ggttgaaatc acgatttttg cccggtccag gatgggccgg atgatcttcc
      781 tgttcacccc ctcaaccgct gccccaacgg gtcgagggag atagtgtcca ttcaggttcc
      841 ccgggatcac cacatcgtta tggtaggtga ccacatgagg agacgaatct ctgagcacga
      901 aggcaaacag ggggctgggc acatggctat gatatatgtc cgcctcaagg ttgggccgct
      961 tgatgggaat aggaacataa aaaaggtcga tgctgggaat gcggacgaat ccgttattcc
     1021 atcttccgca tgatgaggct acagttacct ggtggcccct cttcgccagg ccgtcggcca
     1081 gctccttcac atgatactca accccgccca gatgaggggg aaaataggga gatacctgaa
     1141 tgatcttcat ggattggagc ggatattggg agtacaacga acttaaagct aatgcaattc
     1201 agctatgtat cagccacctt tagcatgatg tcagaggagc tatattcagt atctcctgag
     1261 gcattaaagc ccctctggct catcagcagg tctatctgtt gatctatgga ggtcttgagg
     1321 cggaagctct ccgccagtga gttatcgaat gaatactcgg atgcgtggcg gccatccctg
     1381 accaggacgg tgatctggta gtcgtcggcg gcgaggcccc gggtcgacca gctccagcta
     1441 ttcgactctg accatctaat catatccttt cctccaagct ggaacttgta taggatggga
     1501 tcgttgtccg ggtccatggc ctccgccttc cagacgatgg tctcccccag aacccgaggg
     1561 ctggagagat ccggcacaag gcttgtgagg acgggaggca gatttttgtc cattagtgtg
     1621 aattcagcat ctattgagtc atcgaaagag tccgtagggg cgtgcttgcc gtccctggcc
     1681 aggacagtga tcctatgtgt gccgggaaga gcatcagatg tgtcccaggt ccaggaagga
     1741 gaagaggacc agccggtcat atcctggcca tcgaggagga acttatagga tagctcgtct
     1801 ccatcaggat ctgcagcttt ggcagtccaa tttatgatgg cgcctatttc gagtggactg
     1861 attgcatccg gcttcagctc tgcgaacacc gggatctggt tgagggatgc agtagaggat
     1921 gcgctcgctg gaacatctgc tggctttgat ggatcttcgg gagagattgc taaaggtgca
     1981 actgatgatt ctgcagatga ttctcctatt gtcgattcat tcagggtata aggccgattc
     2041 atctcgagat caatggagtt ctggctggca tgagggccgt ccattatctg aacggtgatc
     2101 ttatgatctc ctgccaccac tccagagcta ttccataccc aggaattcga tggcatccag
     2161 ccagtcatat cctggccatc gaggaggaag cggtatgata tctcatcctg ctctggatct
     2221 attgcagtgg cggtccaggt gatctgggat ccctgagggc tggggctctc aggatctgac
     2281 tccagcccct tcagaatcgg tggctggttt tgttccagga tgaccatggt ggcattcaaa
     2341 gagctatcaa atgagtcaga agatgaatga aggccatccc gggccaggac ggtgatgata
     2401 tattcgcccg cgggcagata atgcgtgaga aagctccagc tgccggatct tgaccaattt
     2461 ttcatctctt caccattgag gaggaagcga tagaggatcc tgtcaccgtc cggatcnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttt ccgtttcagt tgcagcggat
     2641 tgtggctttg atttggcaga agaggagctg gaggctgcag atgcgctaac ctgggcgaca
     2701 gagctgccac cgcctccggc actcaggacg gtgacctttg tcttctcttc aaatgagacc
     2761 ggcaagccat cagagctcgc cattgattcg tttgtgctgt tttccatttc ttcctgatcc
     2821 tcggactgat catcaacaag atcgaccaga agatgctcag atttgtaggg agtagtgctg
     2881 tccccttctt tgaataggag gagactaagc ctctgccggc ctggctgtcc ctcaagcaca
     2941 aagcccagca tgctctccca ggtctcatta tcagccagac gcaggccttt gacgaagatg
     3001 gtggaattgt caaaggccag ccggagggag taattgactg gaatgccttc atggttgtcg
     3061 atcctcgccc gcgcttccag gtcgcggcca gcggtaagag tcgtgtattg cccttcggat
     3121 gaaccgtcaa gctcgctttt cggccaggtg acctccagat cggtatagcc cttgggagag
     3181 gataagaaat tcatgccagg ttgataatga taaaaggcca gggccaggcc agccagaacg
     3241 aggatcgcgg ccagggcgat cagaagaggg ccccgggagg agacgaagcc tgatgagatg
     3301 cggccagagg tcctgcgact gcgatagctc ctcttcgctc cgatcacaaa cctcttgttg
     3361 cgaggcagag ccgaccagcg gagataggat agggcggcaa gaaatagcac aagaaaagag
     3421 agaatagtgg ccagggatgc agcctggagc cccctggggg taaaatacag aatcagactg
     3481 atgagccctg ccaataacag agaggccccc agagagagaa gaaagcgtct attcaaatcc
     3541 aggtcgccag cggcagggaa gagggagatg actgcaaggt agccgggtat cagtatcacc
     3601 agagctaaag caagatatga gccggcctct ttcagcggtg gagcaaaaag attagccaga
     3661 gcaatcgctg ataaagagat cagaatggat gcgataaggt accagggaat ctgcctggca
     3721 gtcctcaaat cattatccct cctttaataa agcctggaag tccatttggc aacaagcaat
     3781 aaaacctttt tccttgaatt cttcataact cgatctcgat tcccaccggg cagtggtcgg
     3841 agccatagac atcatcgagg ataaaagccg atttcacccg atcccgcatg cctaaagaga
     3901 cgaagaagta gtcgatcctc cagcccacat tgcgctcgcg agccctggtt ttcatatccc
     3961 agaaggagta tttttcccct tcaggatgaa agaggcggaa ggtgtccagg aagccgtgct
     4021 ctatcaatcg gtcgatccag gccctctcct ccggaaggaa tcctgatatc ttctcattcg
     4081 gcttgggccg ggcgagatcg atctccttat gggcggtgtt aacatcgccg cagatcacaa
     4141 tatctctgcc ctcggccaca agccggtcca tgagatcgag gaacagatca tagaacctca
     4201 tcttatagct caacctctcc tttgaggcct tgccgttggg gaagtagaca ttgaatagca
     4261 gaaaatcccc atagtcggca acaatcgccc tctgctcatc atcaaagcca acaaagctca
     4321 atgagtactc caggctcagg ggtttggtct tagtgaaaag ggccactccg tcccgcccct
     4381 ttctggcgcc cggactgaaa aacgatatgt atccggggag gcctttgagc tcttttggaa
     4441 tctgatcctc tgtcgccttg atctcctgga ggcagagcac atcgggacgg tctttaagaa
     4501 gccagtccac aaaacccttc ttatagatgg ctctcaagcc gttcacattc caggacagaa
     4561 ttctcatgga tggcattctt tcccctcaaa tgagagttat tgccatccga ttaattcctt
     4621 gcgattgtat ttcgattgtg aggaagatat gtccacac
//
LOCUS       DAOSGP010000091         4650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_16513_length_4650_cov_18.186507, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000091 DAOSGP010000000
VERSION     DAOSGP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16513_length_4650_cov_18.186507"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            540..>1153
                     /locus_tag="PLV49_03790"
     CDS             540..>1153
                     /locus_tag="PLV49_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOG97999.1"
                     /translation="MSEGKFALVYHPIYLLHDTDGHPERKERLTAILDRIESEGLDIE
                     FITPKSATIEQVEAIHGRNYIDQVKAICERGGGHLDIDTILSKDSFDAALMAAGGAIA
                     AVDAVIEGHRGAFALVRPPGHHAMPNRGMGFCVFNNVAIAARHAQSRDLEKVLIVDWD
                     VHHGNGTNAIFYSDRSVLYFSTHQFPHYPGTGRMNDVGEDGAEGT"
     assembly_gap    1154..1253
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1254..2238)
                     /gene="cca"
                     /locus_tag="PLV49_03795"
     CDS             complement(<1254..2238)
                     /gene="cca"
                     /locus_tag="PLV49_03795"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOG98000.1"
                     /translation="MLSQVRPSPKERERLRLISREIIAKIERLAEERGMVLQAMLVGS
                     AARDTWLAQDHDLDIFLGVPENSDLSEAMELARLVAPEHEERYAEHPYVHAWVDGFEV
                     DLVPCYLVDDASRLKSAVDRTPFHTRYIKGKIAGLEDDVLLLKQFLKGIGVYGSELKT
                     GGFSGYLVELLVIHCRSFPDVLRAASHWRPDTVLDLEGKGKKLHDEPLTVVDPVDPNR
                     NVAAALTLDKMLLFSAASRSFLDRPSLEFFFPREDEPLSDEEAQARMDARGTMPIILQ
                     FQAPDVVEDVLYPQMRKAEESIRALLTRKGFLILRSDVLAYRDRAVMLFEME"
     gene            complement(2252..2797)
                     /gene="thpR"
                     /locus_tag="PLV49_03800"
     CDS             complement(2252..2797)
                     /gene="thpR"
                     /locus_tag="PLV49_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOG98001.1"
                     /translation="MTGIRCFVAVDLPEEMREKVGPLQSKIATEGLRLVRPELVHVTI
                     KFLGDVPAEKVERVKKALAAVRAAPFTARLVGLGAFPGRSVRVVWLGLEGDFSQLSAE
                     VQAATKSLGFPPEERGFTPHVTIGRVGRPNAGTSRELLAKIGLQSTVDLGSFTVEEFY
                     LKKSTLTPGGPIYEDLAKFPL"
     gene            complement(2794..4449)
                     /gene="ilvD"
                     /locus_tag="PLV49_03805"
     CDS             complement(2794..4449)
                     /gene="ilvD"
                     /locus_tag="PLV49_03805"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOG98002.1"
                     /translation="MRSDVTKVGRERAPHRALLKATGLCDEEIRRPFIGVVNSSNDFI
                     PGHIHLDRLGEAVKAGILSAGGVPFEFQTIGVCDGIAMGHKGMRYSLPSRELIEDTIE
                     IMAESHQLDGLVMIPTCDKIVPGHIMAAGRLDLPTIVVTGGPMMPGFACDKELDLINV
                     FEEWQKGGETLDALEDMACPGAGSCAGLFTANTMACLTEAMGLSLPGCATAHAVDARK
                     IRLAKLSGMKIMELVEKGITARQIVTAASLDNAIRVDMAIGGSTNTVLHLMAIAAEFG
                     LDLDIERFDQLSRETPHLVNLRPGGPHHIIDLERAGGVPAVMSALRSRLALDALTVTG
                     QRVGENLDSFKLINPLTNSRVISTINKPVHAEGGIAILFGSLSPEGSVVKQTAVSPSM
                     LVHSGPAVVYDSEEASMAGIVGGEVKPGDVVVIRYEGPKGGPGMREALAPTSAIAGAG
                     MSESVALITDGRFSGGTRGPCIGHVSPEAAVGGPIALVRPGDMISINIPARKLDLLVD
                     PAELERRRGEWRMPDMPENAIKTGVLARYRKSVTSASKGSVLR"
BASE COUNT          952 a         1331 c         1212 g         1055 t
ORIGIN      
        1 gccatcgctg ttgccagtaa tagcaccatc gatgtaaatg taattgcagc caatttcttc
       61 atcttttaac ctcctattta cccttcatga tcacttttag cagcatacag tctgattatt
      121 cttaaaacta ttgcctgaat aacgtctcga accgcccgct aaaggcatgg cgcacggtat
      181 ctcctgcatt gccattgctt tcgggcgcat ctctgacatg tgtatagcta cgtcaccctc
      241 ctaatcccat aagggactat agggtagtga tagagtattt ccctattata tcttttttgg
      301 tcaatgatag cagcttggtg ctggtctttc ccacacagag ctaagaggtt gaccaaagag
      361 ctttgctagg ctcaaagcct gcattatctg ccttgtttaa aagcttattc tttcagctca
      421 gaaggaaaca ctgatcattt tcttcgtatt atttctaaat acattctctt ccaaaatatt
      481 gaaaaatttt gatttttaaa gggaaaatcg ttttacgaga tgcggtatta tatgcaaata
      541 tgagtgaagg gaagttcgcc ctggtctatc atcccatcta tctattgcac gataccgatg
      601 gacatcctga gagaaaggaa cgcctgacgg caatcttgga tagaatcgaa tccgaagggc
      661 tggatataga gttcattacg ccgaaatccg cgacgatcga gcaagtcgag gcaatacacg
      721 ggcggaatta tatagatcag gtgaaggcca tatgcgagcg gggaggcggc cacctcgata
      781 tcgataccat cctctccaaa gactcttttg atgccgccct catggcagcg ggtggtgcta
      841 ttgctgccgt ggatgcggtg attgaaggcc ataggggagc ttttgccctg gtcaggccgc
      901 ccggccacca tgccatgccc aatcgcggca tgggcttttg cgtcttcaat aatgtggcca
      961 tagcggcaag acatgcccag tccagggatc tcgagaaggt tttgatagtg gattgggatg
     1021 ttcatcatgg taatgggacc aatgccatat tctactccga tcgttcggtg ctgtatttct
     1081 ccacccacca attcccccac tatccgggca cgggcagaat gaacgacgtc ggggaggatg
     1141 gcgcagaggg gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctccat
     1261 ctcaaagagc atcaccgccc tgtcgcgata ggcgaggaca tcgctgcgca gtattaaaaa
     1321 gccttttcga gtcaagagcg ccctgatgga ttcttccgct ttgcgcatct gggggtagag
     1381 gacatcctca accacatcag gggcctggaa ctggaggatg atgggcattg tccctcgcgc
     1441 atccatcctg gcttgagcct cctcatcgga gagaggctca tcttcgcggg ggaagaagaa
     1501 ctccaggctg ggccggtcga gaaacgagcg ggatgcggca ctgaaaagga gcatcttgtc
     1561 cagggtcagc gccgcggcca cgttccggtt ggggtccacg ggatccacca ccgtcagagg
     1621 ctcatcatgc agcttctttc cctttccctc caggtccagg acggtatcgg gcctccagtg
     1681 agaggcggcc cggagaacat cgggaaaaga gcggcagtgt atcaccagca gctccaccag
     1741 gtagccggaa aagccgcccg tcttcaattc tgagccgtag acccctattc ccttgagaaa
     1801 ctgcttcaaa aggaggacat catcctccag gccggcgatc ttgcccttga tatatctggt
     1861 atgaaatggg gttctgtcca ccgctgactt cagccggctg gcatcatcga ccagatagca
     1921 gggaaccaga tccacctcga agccatccac ccaggcatgg acatatggat gctcggcgta
     1981 cctctcctcg tgctccgggg caaccagcct ggccagttcc atggcttcgc tcaggtcgct
     2041 attctccggc acacccaaaa aaatgtccag atcatgatcc tgggccagcc aagtgtctct
     2101 ggccgccgag cccaccagca tggcctggag gaccatgccc cgctcttctg ccagacgctc
     2161 gatttttgct atgatctccc tggagataag acgaagcctc tctctctctt tcgggctggg
     2221 ccggacctgg ctcagcacca gttctcgaat ctcagagagg gaactttgcc aggtcttcgt
     2281 agatgggtcc tccgggggtc agtgtgctct ttttgagata gaattcctct accgtgaagc
     2341 ttcccaggtc cactgtagac tgcaggccga tcttcgccag aagctcccgg ctggtcccgg
     2401 cattgggcct gcccaccctt cctattgtca catggggggt gaagccccgc tcctccgggg
     2461 gaaatcccaa ggattttgta gccgcttgta cctcggctga taactgggag aagtcccctt
     2521 ccaggcctag ccagaccacg cgcaccgatc ttccgggaaa tgcgcccaaa cccactaagc
     2581 gggcggtgaa gggggccgcc ctgaccgcag ccagagcctt tttgaccctc tccaccttct
     2641 ccgcgggcac atcccccagg aacttgatgg ttacatggac cagctccggc cggaccagcc
     2701 gcaagccctc cgtggctatc ttgctctgca gtggtccgac cttttcgcgc atctcctctg
     2761 ggagatccac cgctacgaag cacctgattc ccgtcatctg agtacgctgc ctttactggc
     2821 cgaggtcacc gacttcctgt aacgcgccag aactccggtc tttatggcgt tctccggcat
     2881 atctggcatc ctccattcgc ctctcctccg ctccagctcc gccgggtcca ccagcagatc
     2941 gagcttgcga gcagggatat ttatgctgat catatcacca ggccggacta gagcgatggg
     3001 accgcctact gctgcctcag gagagacatg gccgatgcag gggcctctgg tgcctccgct
     3061 gaaccggcca tcggttatca gggcgacgga ttcgctcatc cctgccccag cgatggcaga
     3121 ggtgggcgcc agagcctctc gcattcccgg acctcctttg ggcccctcgt atcttatcac
     3181 caccacatct ccgggcttta cctctcctcc tactatgccg gccatagaag cctcttcgct
     3241 atcatagact actgccgggc cggaatggac gagcatggat ggggatactg cggtctgctt
     3301 gaccacggat ccctccgggg acaggctgcc aaagagtatg gctatgcccc cttcggcatg
     3361 cacaggttta ttgatagtgc ttatgacacg ggagttggtg agggggttga tcaatttgaa
     3421 tgagtccagg ttctcgccca ccctctggcc ggtgacggtc agggcatcca gggccagtcg
     3481 agagcgaagg gcgctcatga cggccggaac ccctcctgcc ctctccaggt cgattatgtg
     3541 gtgaggcccg cccggcctga ggttgaccag atggggcgtc tcgcggctga gctgatcgaa
     3601 gcgctctata tcgaggtcca ggccgaactc cgctgcgatc gccatcaggt gcagaactgt
     3661 gttcgtcgat cctccaatgg ccatgtccac ccggatggcg ttatccagag aggctgctgt
     3721 gactatctgg cgggcggtta tgcccttttc caccagctcc atgatcttca ttccagagag
     3781 cttggccaga cggatctttc gcgcatcgac cgcatgggcg gtagcgcacc cgggcaggga
     3841 gaggcccatg gcctcggtga ggcaggccat ggtattggcg gtaaataggc cagcacagga
     3901 gcctgcgcca ggacatgcca tatcctccag ggcatccagg gtctcgcccc ctttctgcca
     3961 ttcctcgaag acattgatca ggtcaagctc cttatcgcag gcaaagccgg gcatcatcgg
     4021 accgcccgtg accacgatgg tgggcaggtc cagcctcccc gcggccatga tatgtccggg
     4081 cacgatctta tcgcaggtgg ggatcatcac caggccgtcg agctggtggg actcggccat
     4141 gatctcaatg gtgtcctcga tcagttcgcg actgggaaga gaatatctca ttcctttgtg
     4201 gcccatggct atgccatcgc agacccctat ggtctggaac tcaaatggaa ctcctccggc
     4261 agagaggatg cctgctttaa ccgcttcccc cagtcgatcg agatgaatgt ggccgggtat
     4321 gaaatcgtta ctagagttca caacgccgat gaagggacga cgaatctcct catcgcacag
     4381 gccggtagct ttgagaagag cccgatgagg cgctctctct ctgcctacct tggtgacatc
     4441 gcttctcata tccaaaacct ccttcgcctc tgccactttt gcgcttaagt agttgctgtt
     4501 aaatacctcc agatacttgc cgccagatgg tgtttgaaaa tcatggatgc ctattcattg
     4561 ttcctggtat tcctggcaat ctacatagcg atcttgcttg gaatcggcct gtatttctcc
     4621 cgcaggcaga ggtcggagac cgacttctgg
//
LOCUS       DAOSGP010000092         4533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_17154_length_4533_cov_18.583519, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000092 DAOSGP010000000
VERSION     DAOSGP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17154_length_4533_cov_18.583519"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLV49_03810"
     CDS             complement(<1..428)
                     /locus_tag="PLV49_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOG98003.1"
                     /translation="MKVAITYHEDFGRHGFSVLKERIQPSFEKLMESGLVDGIEVQVF
                     RAKPAPIELVAKAHTEAHMANMETDQYRDVAILSAGSVMMAAEMVASGQAESAFAFTG
                     TAGHHASRGSCWGFCYFNDVAIAILRLKEMGFERFLIVDV"
     gene            complement(446..1960)
                     /locus_tag="PLV49_03815"
     CDS             complement(446..1960)
                     /locus_tag="PLV49_03815"
                     /EC_number="2.4.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP phosphorylase"
                     /protein_id="HOG98004.1"
                     /translation="MLLVRPFDIEIGQHKVMLNIVDARERGLNPGDRVRVRAKGAATT
                     ALLDTTTQMVKPGEIGIFTEALKDLNNPVDVDILPAPRPASICHIKSLMDNEKLSEDQ
                     IRTIVQDIVDNNLSDIELAAYVTASYIHNMSSEEVVWLTKAMIETGERIHYDTHPVMD
                     KHSIGGVPGNKVSLLIVPIVAAAGLLIPKTSSRAITGAGGTADLMEVLAPVEFSAAEI
                     KEITEKVGGVIVWGGATNIAPADDKLIKAEYALSIDPYSQMLASIMAKKGAVGADAVV
                     VDMPVGPGTKLPTAEDGRTLAKDLIDLGDKLGIRVECAMTFGGSPVGRTIGPALEVRE
                     ALTLLENKPGPNSLREKSLSLAGILLEMGGVAGRGEGYAAAEEILSSGKAHAKLMEII
                     AAQGGNPNVKSEDIVIGENCEEILAPAGGYVVAFYNKRLVELARMAGAPSDQKAGVII
                     HKKMGERVKKGEPLITICSSSDWELDSAVRDAKRSMPIVVEGMLLERYPRITEL"
     assembly_gap    1977..2076
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2077..2663)
                     /locus_tag="PLV49_03820"
     CDS             complement(<2077..2663)
                     /locus_tag="PLV49_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOG98005.1"
                     /translation="MARIRDFFETNEGWIFAVSDYNHPHGLRSLLRYIPDPSGERAAR
                     GTRYRKMDFDQAFEFLRQNRPDYIQDLHVVPESDVLQLYDPSQGLRAVAEKDPKTEKI
                     ALILNQAGVPWEEMGITGSRLIGLQAPASDIDFVVYGPMWWKARDIVDRSKREGVIQD
                     LDEDTWKKIYAKRKPEISLEEFMLHEKRKGNRGMI"
     gene            complement(2663..3235)
                     /locus_tag="PLV49_03825"
     CDS             complement(2663..3235)
                     /locus_tag="PLV49_03825"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG98006.1"
                     /translation="MIPSAARFIIGGGVVVYPTETVYGLGANALDEQAVMRVFLIKKR
                     PLSMPISLAVSSLEMLRDVAEVRDEDMDILEDLLPGPVSILLRKKSIVPDILTAGSSL
                     VGIRFPDHESALRIIDKAGPITSTSANRTGQPAPVSAKEVSKEIEDRVELVVDGGRCK
                     YCQPSTLLDLEKREIIRPGAGLDKALKAIK"
     gene            complement(3239..3727)
                     /locus_tag="PLV49_03830"
     CDS             complement(3239..3727)
                     /locus_tag="PLV49_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HOG98007.1"
                     /translation="MTEKQCEICGAEISGSPQKIVIDRSTMEVCKSCARFGKPEDKWT
                     PVPRKMVPVERAFTVMRPKPRDQFKDLVELVPDFGRKIREAREGLGLTPEQLGARIKE
                     KATLLKKIEREDISPEDEVRKKLERELKIKLTDQASEAQVKSGGSGRGLTLGDIANIK
                     RK"
     gene            3791..>4533
                     /locus_tag="PLV49_03835"
     CDS             3791..>4533
                     /locus_tag="PLV49_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG98008.1"
                     /translation="MNESQAGADFSRYILDRMRQLEERNLALREQKDRVEGEKRLIEN
                     QKLKFEREARKLRSELERLRVGPMIVGTIVDVLDENRVIVKSSTGPRFVVSLSQFIEE
                     EIKVGAQVGLNQQSFAVMCVLPSPRDPAVFGMEIEEAPDVQFSQIGGLDTQISEIREI
                     VELPLKRPDLFTSVGIEPPKGVLLHGPPGTGKTILAKAVAQSTEATFMRVVGSEFVQK
                     YIGEGARLVRELFELAKSKSPAIIFIDEL"
BASE COUNT          904 a         1316 c         1147 g         1066 t
ORIGIN      
        1 tccacgtcca ctatgagaaa gcgctcgaag cccatctcct taaggcgcag tatggcgatc
       61 gctacgtcgt tgaagtagca gaatccccaa cagcttcctc ttgaagcatg gtgcccggcg
      121 gttccggtga aggcgaaagc ggactctgcc tggccagaag ccaccatctc tgcggccatc
      181 atcacgctcc ctgcggacaa aatggccaca tcccggtatt gatccgtctc catgttggcc
      241 atgtgcgcct cggtatgagc cttcgccacc agctctatag gagcgggttt ggcccgaaac
      301 acctgcacct caatcccgtc aaccagcccg gactccatca gcttctcgaa ggatggttga
      361 atcctctcct tcagaacgct aaagccatgc ctgccgaaat cctcatgata tgtaattgca
      421 accttcatag ggcgaccccc aaattctaaa gctcggttat gcggggatac ctctccagca
      481 gcattccttc taccactatt ggcatagacc tcttggcatc cctgactgcg ctatccagct
      541 cccagtccga tgaggagcag atggtgatca gaggctcgcc tttctttacc ctctcaccca
      601 tcttcttgtg gattatgact cccgctttct ggtccgaggg agcaccagcc atgcgagcca
      661 gctctacaag gcgtttattg tagaaggcca ccacatatcc tcctgccgga gctaaaattt
      721 cctcgcagtt ctcgccgatg acgatatcct cgctcttcac attgggattg cctccctggg
      781 cagcgatgat ctccatcagc ttggcatgag cctttccact gctgaggatc tcttctgcag
      841 cagcgtagcc ttctccccgt cctgccactc cgcccatctc cagcagtatg ccagctaatg
      901 agaggctctt ctctctcagg ctatttggac ccggcttatt ctccagaagg gtaagcgcct
      961 cacgcacctc cagagccggt cctatggttc tgcccacggg agagcctcca aaggtcatgg
     1021 cgcattccac ccgaattccc agcttatcac ccaggtctat caggtccttg gccagggtcc
     1081 tgccatcctc tgccgtgggc agctttgtgc ccggccctac gggcatatca actaccacgg
     1141 cgtcagcgcc tacagcccct ttcttggcca tgatggaggc cagcatctgg ctgtatggat
     1201 ctatggataa tgcatactcc gcttttatca gcttatcatc tgcaggggcg atatttgtgg
     1261 ctccgcccca gactatgact ccgcccacct tctctgtgat ctccttgatc tctgccgcac
     1321 tgaactccac cggggccaag acctccatca ggtcggcggt accgcctgcc ccggttatgg
     1381 cccgagagct ggtcttgggg atgagaaggc ctgctgcggc tactatgggc acaatgagaa
     1441 gggatacttt gttcccggga acgccgccaa tgctgtgctt gtccatgacc ggatgggtgt
     1501 cataatggat ccgctctccg gtctcgatca tcgctttggt cagccagacc acctcctcag
     1561 aggacatgtt atggatatag gaggcggtga cataagcagc aagctcaatg tcgctgaggt
     1621 tgttgtccac gatatcctgc acaatggttc ggatctgatc ctcactcagc ttctcgttat
     1681 ccatcaagct tttgatatga cagatggaag caggcctcgg tgcaggcagg atatccacat
     1741 ccaccggatt attgagatcc ttaagagcct cggtgaagat accgatctcc ccaggcttga
     1801 ccatctgcgt agtggtatca agcagggcgg tggttgcagc ccccttggcc ctcacccgga
     1861 cccggtctcc aggattcaag cccctctccc gggcatcaac gatattgagc atgactttat
     1921 gctgacctat ctctatgtca aagggtcgaa ctagcagcat ttttataacc tcttttnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcga tcatgccccg gtttcctttt
     2101 ctcttctcat ggagcatgaa ctcctccagg ctgatctcgg gttttctttt ggcgtagatt
     2161 tttttccagg tatcttcatc caggtcctgg atgacaccct ctctctttga ccggtccact
     2221 atatcccttg ccttccacca catgggacca tagaccacga aatcgatatc cgaagcaggt
     2281 gcctgcaagc ctatgagcct ggagccggtg attcccatct cctcccaggg cacacccgct
     2341 tgattgagga tgagggcgat tttctccgtc ttgggatcct tctctgccac agctctcaag
     2401 ccctgggagg gatcatagag ctggagtaca tcggattcag ggaccacatg aaggtcctga
     2461 atataatccg gcctattctg gcgcaagaac tcgaatgcct ggtcgaagtc catctttcgg
     2521 tagcgcgtgc ctctggcagc acgctctcct gacggatcgg gaatgtagcg gagcagggag
     2581 cggaggccgt gagggtggtt gtagtctgag acagcaaaga tccagccctc attggtttcg
     2641 aaaaagtctc tgatccgggc catcacttta tcgccttcag cgctttgtcc agaccagcgc
     2701 ccggccggat gatctctctt ttctccaggt ccaggagggt tgagggctgg cagtatttgc
     2761 atctgccacc gtccaccacc agctccaccc gatcttctat ctcttttgag acctccttag
     2821 cgctcaccgg cgctggctgg ccggtgcgat tggcgcttgt ggaggtgatc ggtccggcct
     2881 tatcgattat cctgagggca gactcatggt ccgggaacct gatgcctacc agggatgagc
     2941 cggcggtgag aatgtccgga actatgctct tttttcgcag caggatggat actggtcccg
     3001 gcagaagatc ctccaaaata tccatgtcct catcgcgaac ctcggccacg tctcttagca
     3061 tctccaggct cgagaccgcc agagagatgg gcatggaaag aggccttttc ttgatcagaa
     3121 agaccctcat aactgcctgc tcgtccagag cattggcccc caggccataa acggtctcag
     3181 tgggatagac taccacaccc cctccaatga taaatcgagc tgcactgggt atcatggatt
     3241 atttccgctt gatgttggct atgtccccta aagtgaggcc cctgccggag cccccggact
     3301 tgacctgagc ctcgctggcc tgatcggtga gcttgatctt aagctccctc tccagcttct
     3361 ttctgacttc atcctcaggc gagatatcct ctctctcgat cttcttcagg agcgtggcct
     3421 tctccttgat cctcgctccc agctgctctg gcgtgagtcc caggccctcc cgagcctctc
     3481 tgatcttgcg cccgaaatcc ggaaccaact ccaccaaatc cttgaactgg tcgcgtggtt
     3541 taggcctcat gacggtgaag gccctctcca caggaaccat cttccgcgga actggtgtcc
     3601 atttgtcctc tggttttccg aagcgcgcac agctcttgca aacttccata gttgatcggt
     3661 caataacgat cttttggggt gaacctgaga tttcggcgcc gcaaatctca cattgcttct
     3721 cggtcatttc ggaaaccttt atacgggtca aagtttaata acctttgtaa ttatggatag
     3781 tggagaaggc atgaatgagt cacaggctgg cgcggatttc tcgcgctaca tcctggaccg
     3841 gatgcgccag ctggaggagc gtaacctggc gctgcgtgag cagaaggacc gcgtggaagg
     3901 cgagaagagg cttatagaga accagaagct caagttcgag cgtgaggccc ggaagctcag
     3961 aagcgagctg gagcgcctgc gcgtcggtcc gatgatcgtg ggaaccatcg tcgatgttct
     4021 ggatgagaac cgggttatcg tcaagagcag taccggcccc agattcgttg ttagtttatc
     4081 ccaattcata gaggaggaga taaaggtcgg ggctcaggtt ggcctaaacc agcagtcatt
     4141 tgcggtgatg tgtgttctgc cctctccccg cgacccagcg gtattcggca tggagatcga
     4201 ggaggctcca gatgtacagt tctctcagat tggcggtctg gacacccaga tctctgagat
     4261 cagggagatc gtggagcttc ccctaaagcg gccggacctt ttcacctcgg tcggaatcga
     4321 gccgcccaag ggcgtcctgc tgcacggtcc accaggcacg ggcaagacca tcctggccaa
     4381 ggcagttgct cagagcactg aggccacatt catgcgggtg gtaggctcgg agttcgtgca
     4441 gaagtacatc ggagaagggg cacgcctggt ccgcgagctg ttcgagctgg ccaagagcaa
     4501 gtccccagcg ataatattca tcgatgagct tga
//
LOCUS       DAOSGP010000093         4525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_17202_length_4525_cov_21.081655, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000093 DAOSGP010000000
VERSION     DAOSGP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17202_length_4525_cov_21.081655"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1319)
                     /locus_tag="PLV49_03840"
     CDS             complement(63..1319)
                     /locus_tag="PLV49_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG98009.1"
                     /translation="MRRSSEVGMLLIALCAISCATSSSSLAFSYTLNIFGNANMDDAI
                     NESDIAYLEDVIAGRSPATELSDANLDGIVDGKDIEQVERLIDGTGEQLTLKAYTIYE
                     ELMNVTIPMPVESIVVLNLACAESVRGIKAQDMVVGVGSSLVEGANRDFFPEMIDLPT
                     VGKWSEPDIEAIISLNPDIVIADLRTPDPEKLEDKLEGTGITVVRMGFTYPDYSSSEI
                     MALGYILDRKDDARELNSFAGKYLDLIEERVSSIAESERPRVYPIYYTSADLWKTGSN
                     GSIVDMLCGLAGGENIAHDLTGGTGGMYPTVDPEWVVGENPQIIFTWSSPGGYSLAND
                     TAMRELWTKIVQAPELSRVDAAKDERVHLMTTEITSRPRWFVGLSYLAKWFYPEKFLD
                     LDPEAIHKEYLERFQGLEYQGIFVYP"
     gene            complement(1339..2439)
                     /locus_tag="PLV49_03845"
     CDS             complement(1339..2439)
                     /locus_tag="PLV49_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG98010.1"
                     /translation="MKSNKHLIALALSIGFLLAVSVINASSEETEFPLTITDSAGRVV
                     SLPEPVQGIIVLNTDAAEAVSALGASDLIVGLAEGIKEDYAFQGLKERPSIGTWSDID
                     FEKIGEIGSKGNGTGIIVLGFVYPGKPNGVEAVSKNLEPFSGIVALGLDFYKEENLSR
                     ELRLLGSILGREDRAEELIDWHDRHVAEVEQGAKNLDRPRVFIEGSSRGLGDITTFGG
                     SSASGMLLEKAGGTNVMEEDEAYPKVEWEWVVSQDPEVIIKTDYLKSSDGLPGWSLTS
                     SGDVKALEEMRNDILSRPGAQNISAVKNDRVYVVSAQTLFGLDNVAGLQRLAEILHPG
                     IALEPEEAYGEYLDFMRLEEQKNRTFVYPAID"
     gene            2719..3654
                     /locus_tag="PLV49_03850"
     CDS             2719..3654
                     /locus_tag="PLV49_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG98011.1"
                     /translation="MAMLDIIDLKAHFSSPDGLVKAVNTVDLQIKDNERLGLIGETGC
                     GKTVLGMSIIRLLPSSAGVEGQIIYNGQDLLHLTEEEMRQLRGREIAMILQNPTTSLN
                     PVLKVGDQIAEVISLHKGLGKKEAKARAVQMLEAAMIPEAERRADQYPHELSGGMKER
                     VMIAMGLASHPSFVIADEPTKGLDARTKAEIVNLIRDMTTGRSMLMITHDLAAAGEIC
                     DRIAVMYAGEILEEASAETILNDPQHPYTQGFIRSLPSRGLHPISGSSPSLIDPPSGC
                     RFHPRCPYAMEVCKSEHPQMMRLENGHSSRCFLCG"
     gene            3645..4445
                     /locus_tag="PLV49_03855"
     CDS             3645..4445
                     /locus_tag="PLV49_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOG98012.1"
                     /translation="MRLIEVRDLRKHYSSGLINKQCSRAVDGVSFHIDQGETLGLVGE
                     SGCGKTTVGRLLLRLIEPTSGSIIFDSRDITRLNRKEMRKLRPRMQMIFQDPESSLNP
                     RMRIGESIAEPFRLKLHGKMTKIEIRDRVLELVDVVGLNPEHINRFPHQLSGGQNQRV
                     VLARILAINPEFIVADEPTASLDVSVQAQILRLMMDLKREFGLTMLFISHDMEVVRHM
                     SDRIAVMKEGRITDIVQQMRYSRASDATGERICDRIKGIDPEPLRATI"
BASE COUNT         1099 a         1233 c         1098 g         1095 t
ORIGIN      
        1 gcatgggggc gattcccagg agaatgcatc catctctccc gggatatcat tcgcccatgg
       61 ggctatggat atacaaagat cccctgatat tctagcccct ggaacctctc cagatattct
      121 ttgtgtatgg cctctggatc caaatccagg aacttctccg gataaaacca cttggccaga
      181 tatgaaaggc ccacaaacca ccttggccgg ctggtgatct ctgtggtcat gagatgcacc
      241 cgctcgtcct ttgctgcatc cacccgtgat agctcaggag cctggacgat cttcgtccat
      301 agctctctca tggcagtgtc attggccagg ctgtagcctc caggagagct ccaggtgaag
      361 attatctggg gattctctcc taccacccat tccgggtcca cagtgggata catcccgccc
      421 gttcctccgg tcagatcatg ggcgatattc tcgcccccgg caaggccgca gagcatatcc
      481 acgatggagc cgttggatcc cgtcttccac agatcggccg aagtatagta tatgggatag
      541 acccggggcc tttcactctc agcgatcgat gatacccgtt cctcgatgag gtccaagtat
      601 tttcccgcga aactgttcag ctctcgggca tcatctttcc ggtccaggat atagcccagg
      661 gccatgatct ccgacgaaga gtagtcggga taggtaaagc ccatacgaac cactgtaatg
      721 cctgtgcctt ctaatttgtc ttccagcttc tctggatccg gtgtcctcaa atcagcaatc
      781 acgatatcgg gattgaggct gatgatggcc tcgatatccg gctcgctcca ttttcccacc
      841 gtgggaaggt cgatcatctc cgggaagaaa tccctgttcg ctccctccac cagggatgag
      901 ccgacgccta cgaccatatc ctgcgccttt atgcctcgaa ccgactctgc acaggccagg
      961 ttgagaacca caatactctc caccggcatg ggaatggtga cattcatcaa ttcttcgtat
     1021 atggtgtagg ccttgagggt gagctgctct ccagtcccat caatcagcct ctctacctgc
     1081 tcgatatcct tcccatccac aataccatcg agattggcat cagatagctc cgttgctggg
     1141 ctcctgccgg ctatcacatc ctccagataa gcgatatcag actcattgat ggcatcatcc
     1201 atattggcgt tgccaaatat attcagggta tagctgaaag ccagcgaaga ggaggatgtt
     1261 gcgcatgata tggcacataa cgcaatcagg agcatcccaa cttcagatga tcttctcatt
     1321 tctatcagac ctcaattctt aatcaatagc cggatagacg aatgtcctgt tcttctgctc
     1381 ctccagtctc atgaaatcaa gatactcccc ataggcctct tctggctcga gagcgatccc
     1441 gggatggagg atctctgcca ggcgctgcag gccggcaaca ttatccaggc cgaatagggt
     1501 ttgggcggat acgacataga cacgatcgtt cttcaccgct gagatgttct gggctccggg
     1561 acggcttaat atatcgtttc tcatctcctc cagagctttt acatcgccag aggatgtcag
     1621 gctccatccc ggcaggccat cggaagattt gagatagtca gtcttgataa tgacctcggg
     1681 atcctgagag acaacccact cccattccac cttgggatag gcttcatcct cctccatcac
     1741 attggttcct cccgcctttt ccaggagcat tcctgaggcc gaactccctc caaatgtggt
     1801 gatatcgcca agcccccttg agctgccctc gataaagacc cttggacggt ccaggttctt
     1861 tgccccctgc tcaacctctg ccacatgccg gtcatgccag tcgattagct cttctgctcg
     1921 atcctcccgg ccaagtatcg agcctaaaag cctcaactcc cggctgaggt tctcctcttt
     1981 gtagaagtcc agccccaggg cgacaattcc gctaaatggc tccaggttct tggaaactgc
     2041 ctcaactcca ttcggctttc caggatagac aaatcctaag acgatgatgc ctgtgccatt
     2101 tcccttgctg ccaatctctc cgatcttctc aaagtctata tcgctccatg tgccaatcga
     2161 tggcctttcc ttcagtccct gaaatgcata atcctctttt attccctccg ccaatccaac
     2221 aatcaggtcc gaggctccta gagcagatac ggcttccgca gcatcggtat tcagaacgat
     2281 gatcccctgc acgggctcgg gaaggctcac cacccttccc gcagaatcgg ttatggtcaa
     2341 aggaaattcg gtctcctctg agctggcatt gatgacgctg accgccagca aaaatccgat
     2401 cgacaatgcc aacgctatta gatgcttatt actcttcaat ttgaacacct tccatttcaa
     2461 tttctggata gatattatct ttaaatgcta ctatttcggt catgatatta aatattgtta
     2521 tcttctaata tacatataat actttcggcc cttttatgta cgaatattaa acatcaatga
     2581 gcaaggaata ttcatctaga gcgaatagaa ttgttgtggt gatgcaagtt cacccaattt
     2641 aatatttttg ttaataatga gtgataaatg cgaattgtgt agcaatcttt attatgatga
     2701 aatcatctta gtctatcaat ggctatgctc gacataattg atcttaaggc ccatttctcc
     2761 tccccagacg gcctggtcaa agcggtaaat actgtggacc tgcaaataaa agacaacgag
     2821 cgattggggc tgatcggcga gaccggctgc ggcaagactg tgcttggcat gtccatcatt
     2881 cggctgctgc cctcctcggc cggggtggaa ggccagataa tctacaatgg acaggacctc
     2941 ctccatctga cagaggagga gatgaggcag ttgcgaggga gggagatcgc catgatcctg
     3001 cagaatccca ccacctccct caatcccgtc ttaaaggtgg gagaccagat cgctgaggtg
     3061 atcagtctgc acaaaggcct gggcaaaaag gaggcaaaag ccagagcggt gcagatgctg
     3121 gaagcagcca tgattccaga ggcggagagg agggcggacc agtatcccca tgagctctcc
     3181 ggagggatga aggagagggt gatgatcgct atgggcctgg ccagccatcc ctcttttgtc
     3241 atcgccgatg agcccaccaa ggggcttgat gcccgcacca aagcggagat cgtcaacctg
     3301 ataagggaca tgaccaccgg gcgatcgatg ctcatgatca cacacgatct tgccgctgct
     3361 ggtgagatat gcgaccggat tgcagttatg tatgcaggtg agattttgga ggaggcttcg
     3421 gcagagacca tattaaacga tccccagcac ccctataccc agggattcat ccgctcactg
     3481 ccaagccggg gattgcatcc catatccgga tccagcccat ccctcataga tcctccttct
     3541 ggctgcagat tccatccccg ttgcccctat gcaatggagg tctgcaagag cgagcatccc
     3601 cagatgatgc ggctggagaa cgggcattcc tcgaggtgtt tcttatgcgg ctaatagagg
     3661 ttcgagatct gagaaagcat tattcctctg gattgataaa caagcaatgc tctagagcgg
     3721 tagatggcgt ttcatttcat atcgatcagg gtgagaccct tggcctggtg ggagagagcg
     3781 gctgcggcaa gaccaccgta ggccgtctgc tcctgcggct catcgagccc acatccggca
     3841 gcataatctt tgatagtagg gacataacca ggctgaatag gaaagagatg agaaagctcc
     3901 gccccaggat gcagatgatc tttcaggatc ccgagtcatc tctcaatccc aggatgcgca
     3961 tcggagagag catagctgag cccttccggc taaagctgca tggaaagatg actaaaattg
     4021 agattagaga tcgggtttta gagcttgtag atgtcgtggg cctgaatcca gagcatatca
     4081 accgctttcc tcatcagctc agcggcgggc agaaccagag agtagtcctg gccagaatcc
     4141 tggcgataaa tccggagttc atcgtcgcag atgagccaac cgcctcgctg gatgtctctg
     4201 ttcaggccca gatattgagg cttatgatgg atctgaaaag ggagttcggc ctgacaatgc
     4261 tcttcatttc ccatgatatg gaggtggtaa gacatatgag cgatcgcatt gccgttatga
     4321 aagagggaag gataacggat attgtgcagc agatgagata cagcagggca tcagatgcca
     4381 caggagagag aatttgtgac cgcatcaaag gtatcgaccc agagccactg agagcaacaa
     4441 tatgatcagt atcaataaaa tcgatagcct ctgccccagg ccggatccgc tctcaatcac
     4501 cttatgatca atgctccctg gaatt
//
LOCUS       DAOSGP010000094         4514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_17280_length_4514_cov_17.572774, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000094 DAOSGP010000000
VERSION     DAOSGP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17280_length_4514_cov_17.572774"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1534)
                     /locus_tag="PLV49_03860"
     CDS             complement(80..1534)
                     /locus_tag="PLV49_03860"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOG98013.1"
                     /translation="MYLSNQSLLISDIMNSMDRFDLIVIGSGAGALVAAQAARAGRNT
                     ALVDQGPMGGTCLNNGCIPSKMLIHPADIIRSIQEAGAVGVHARIEKIDFPRIMNRMH
                     KVVDEGRAQMEATIRSRENLTHYQERAEFIDEYVLKAGSRTLTATQFVIASGSRSRVP
                     PISGLEESGYIDNVSLLNLREPPRSLIIIGGGYIGCEYGHFFSAMGTEVTILGRSPRL
                     LCGEDPEISERLHESFSRYCRVVTGYEVLSVERNGEKKVVSARGTKDGKIRQFEGEEV
                     LLAAGRSSNSDLLRPERTGVEIDRQGWIWVDEYLQTSKKGIWALGDAIGKHMFRHTAN
                     YEARIVAHNILLAQGKAERKAADYHAVPYAVFSHPTVAGVGMKESEAKARGLKVLVGR
                     ARYTDSAKGVAMAEEYGLVKVVLEEETGRILGASIIGPEAPELIQQVVYLMNTDRQDL
                     STAMRSQLIHPTINEVLGRAFSGLERSRNREQPK"
     gene            complement(1590..2090)
                     /locus_tag="PLV49_03865"
     CDS             complement(1590..2090)
                     /locus_tag="PLV49_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98014.1"
                     /translation="MRIDYRTRLDEAVSLADIFEVVKTLVEESMNKRRGGLMLGMANL
                     GNDPHGFLGGFFTTGSNVIVLNKIPLNRIMETRPELYKPYAFHVLLHEYIHSLGYLDE
                     TEVQEKVHQITREALGEEHLATQFAARASGFIKHLAYPHVSWKADDEGIELVEGFDRS
                     SVSYIA"
     gene            complement(2180..2479)
                     /locus_tag="PLV49_03870"
     CDS             complement(2180..2479)
                     /locus_tag="PLV49_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HOG98015.1"
                     /translation="MIEGLKGEEGSLKEDLSIETVKWQEKAESLFGQLSGDERMLENI
                     SSYLSDSHHFLKQNDLIRAFEAVIWAWAWMEIGLEKSLLHRGPEEEISDQDPLIG"
     gene            complement(2501..3163)
                     /gene="queC"
                     /locus_tag="PLV49_03875"
     CDS             complement(2501..3163)
                     /gene="queC"
                     /locus_tag="PLV49_03875"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOG98016.1"
                     /translation="MKAVLILSGGLDSTTLLYKLLADGYLVDALTFNYGQKHKKEIDC
                     AIAITSRLDVPHQIIDLSTLAAFLGESALLGKKEVPSCHYTEEAARQTVVPNRNMIML
                     SVAAGYAEAHEIPELFYAAHKNDQTIYPDCRPEFVEALKPAIRLGTAWNPVELQAPFV
                     HMTKADIVRMGLVLQVPYELTWSCYKGKAMPCRSCPTCIEREEAFALCGCRDPLIGMN
                     SN"
     assembly_gap    3319..3418
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3419..4395)
                     /locus_tag="PLV49_03880"
     CDS             complement(<3419..4395)
                     /locus_tag="PLV49_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG98017.1"
                     /translation="MLYEILIILILILANGAFSMAEMAVVSSRKARLRQMADSGDKGA
                     AVALDLISNSNQFLSAVQTGVTLMATLIGAFGGATLSDELAGYLLGLYPALGQFSHGL
                     ALALVVLSITFVSLVIGELVPKRIALSNPERIASWSASPLNAFSRIVYPVILLLSKST
                     EFVLQALGVNSNAEPEVTEEEIRIMIDQGTMAGVIEEDEQDIMERVFSLGERKVNSIM
                     TPRGKIVWLDIHDTLAEIQRKTVSGPYTMFPVCSKKLDNVLGVIQSKDILNCDLNKKE
                     IDLRSLLLPPLFVPESMRALKVLEKFKQTGIHLAIVLDEYGTVQGIVALV"
BASE COUNT          959 a         1272 c         1110 g         1073 t
ORIGIN      
        1 ccatgggaga ggacataagg cctctttcga atccgcatct gccagagggc tctggccgcc
       61 agccgggggg agaaggatct catttgggct gctcccggtt tctagagcgc tcgagcccgg
      121 agaatgccct ccccagcacc tcattgatgg tgggatggat gagctgagac ctcatcgcag
      181 tggaaaggtc ctggcggtcg gtgttcatca ggtaaacaac ctgctgaatg agctctggcg
      241 cctctgggcc gatgatgctc gcccccagga tccgaccggt ctcctcctct aaaactacct
      301 tcaccaggcc gtactcctca gccatagcca cccccttggc cgaatctgtg tatctcgccc
      361 ttcccaccag caccttcagg cctcgggcct ttgcctcgct ctctttcatg cccactcccg
      421 ccaccgtcgg atgagagaat acggcataag gaacggcatg atagtcagcc gctttccgct
      481 ctgctttgcc ctgagccagg agaatattgt gggctacgat gcgggcttca tagttggcgg
      541 tgtggcggaa catgtgcttt ccgatagcgt ctcccaaagc ccagatcccc tttttgctgg
      601 tctggagata ttcgtccacc cagatccagc cttgcctgtc gatttccact cctgtcctct
      661 ccgggcggag aaggtcggaa ttagagctcc ggcctgcggc aaggaggacc tcctctccct
      721 cgaactgccg gattttgcca tcctttgtcc ctctggcaga gacgaccttt ttttcgccat
      781 tcctttccac cgataagacc tcgtagcccg tgaccacccg gcagtagcgg gagaagctct
      841 catgcagtcg ctctgagatc tcaggatcct cgccacacag aagtcggggg ctgcgtccca
      901 ggatggtgac ctccgttccc atagcggaga agaagtgacc atactcgcag ccgatatagc
      961 ctccgccgat tatgatcagg ctccggggag gctctctcag attgagaagt gatacgttat
     1021 cgatatatcc cgattcctcc aggcctgaga tgggaggaac gcgagaccga gagccgctgg
     1081 cgatgacgaa ttgggtggcg gtgagggttc ttgatcccgc cttcaggaca tactcgtcga
     1141 tgaactccgc tctttcttga tagtgggtca gattctccct tgatctgatg gtcgcttcca
     1201 tttgggcgcg cccctcatcc accaccttat gcatgcggtt catgatccga ggaaaatcga
     1261 tcttctctat tcgggcatgg acgcccaccg ccccggcctc ctggatgctc cggataatgt
     1321 ctgctggatg gatgagcatc ttcgagggga tgcagccgtt gttaaggcag gttccgccca
     1381 tggggccctg gtccaccaat gcggtatttc ttcccgcgcg agcggcttga gcggccacca
     1441 gtgcccctgc gcccgagccg atgacgatga gatcgaatct gtccatgctg ttcatgatat
     1501 cggatatcag aaggctctga ttgcttaaat acatgcggga atatgggagc cgtcgggcaa
     1561 tgggatggaa tagggactca ggacgatctt caagcgatat aagagacgct ggaccggtcg
     1621 aatccttcta ccagctcgat tccctcatca tccgccttcc atgatacatg gggataggcg
     1681 agatgtttga tgaatccgga tgctctggcg gcgaattgcg ttgccaggtg ctcctcgccc
     1741 agggcctctc tggtgatctg gtggaccttt tcctgcacct ctgtctcatc caggtagccc
     1801 agggagtgta tgtactcgtg cagaagaaca tggaatgcgt agggcttgta tagctcaggg
     1861 cgggtctcca tgatcctatt caatggtatc ttattcagga caatgacatt ggagcctgtg
     1921 gtgaaaaagc ctcccaggaa gccatgggga tcgtttccca ggttcgccat gcccagcatc
     1981 aggccgcccc ttcgtttgtt catgctctct tcaaccaagg tctttaccac ctcaaatatg
     2041 tccgccaggg aaactgcttc atccaggcgg gtcctgtaat caatcctcat ttcaaaccca
     2101 tgtagctacg tctatgtata taatagtaac ctgaagttag tttaagctcg aataggctca
     2161 ttccatgaga ggtcttcgat taacctatca gaggatcttg atcagagatc tcttcctccg
     2221 gtcccctgtg caggagactc ttctccaggc cgatctccat ccaggcccag gcccagatga
     2281 ccgcctcaaa ggctctgatc agatcattct gttttagaaa atggtgagag tcggatagat
     2341 aagaggaaat gttctccagc attctctcat ccccggatag ctggccgaag agcgattctg
     2401 ccttctcctg ccacttcaca gtctcaattg agagatcctc tttcaggctt ccctcttcac
     2461 ctttcaggcc ttcaatcatc ttattcaatc accgcaagaa ttaattgctg ttcatcccta
     2521 tcagcggatc cctgcaaccg catagggcaa atgcctcctc cctctctatg caggtgggac
     2581 aggagcggca gggcatagct tttcccttgt agcacgacca ggtgagctca tagggaacct
     2641 gcagcacgag gcccattctc acgatatccg ccttggtcat atgcacaaag ggcgcttgca
     2701 gctcgacggg attccaggct gtccccagcc ttattgccgg cttgagagcc tctacgaact
     2761 ccggccggca gtcggggtag atcgtctggt cgttcttgtg ggcggcgtaa aacaactctg
     2821 gaatctcatg ggcctcggcg tatcctgcag ccacggagag catgatcata ttcctgtttg
     2881 gcaccaccgt ctgcctggcc gcctcctcgg tatagtggca gctgggcacc tccttcttgc
     2941 ccagaagtgc gctctccccc aggaatgccg ccagagtgga gaggtcgatg atctgatgtg
     3001 ggacatcgag cctggaggtg atggctattg cgcagtctat ctctttttta tgcttctgac
     3061 catagttgaa ggtgagggca tctaccagat aaccatcggc cagcaatttg tagagcaatg
     3121 tcgtcgagtc cagaccgccg gatagtatca aaaccgcctt catttccctc ttaattttac
     3181 tggcattcaa cataaaactg cccctcctga ggatccttag ctcgtcacat cctcggccag
     3241 aggggtcacc agcagcttgt ccacccggcg ctcatccata tccatgacct caaagcgata
     3301 gccaccccat tcgaatttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac
     3421 cagtgccaca atcccctgca ctgtgccata ctcatccagg acgatggcca gatggattcc
     3481 cgtctgcttg aacttctcca gcaccttcaa agccctcatg ctctccggta caaatagggg
     3541 cggcagcagc aaagacctca gatcaatctc ttttttatta aggtcacagt tcaggatatc
     3601 cttggactga atcactccca ggacattatc cagcttcttg ctgcagaccg gaaacatggt
     3661 gtacggcccg ctgacggtct tcctttggat ctcagcgagg gtgtcatgga tatcaagcca
     3721 gactatcttt ccccgagggg tcatgatgga gttgaccttt ctttcaccca aagagaagac
     3781 cctctccatg atgtcctgct catcctcttc gatcaccccg gccatcgttc cctggtctat
     3841 cattatcctt atctcttctt cagtcacctc cggctcagcg ttagaattga cccccaaggc
     3901 ctggaggaca aactccgtgg atttgctcaa gagaagaatc acggggtata caatcctgga
     3961 aaaagcgttt aagggagatg cagaccagga tgcaatccgc tccggattgg agagggctat
     4021 cctctttggc accagctcgc ctataacaag gctgacgaag gttatcgaca ggactaccag
     4081 ggctagagca agtccgtggc tgaattgacc taacgcagga taaaggccaa gcaagtagcc
     4141 tgccagctca tcggataagg tcgcgccgcc gaaggccccg ataagtgtgg ccattaaggt
     4201 taccccagtc tgaacagcag aaagaaactg attggagttg ctgatcagat ctaaagcgac
     4261 agcagcaccc ttatctccac tatctgccat ctgacggagc ctggccttgc gcgaggagac
     4321 aactgccatc tccgccatgg agaaggcgcc gttggctaat attagaatta gtatgatcag
     4381 aatctcatac aacatgaact actacccttg caggatggta cgcccggtct gtctccaaat
     4441 tcatgcattt ccctgagatt atgggccgtt ttctcatttt cataagacag gataataaag
     4501 gttatttata gctt
//
LOCUS       DAOSGP010000095         4512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_17291_length_4512_cov_19.213821, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000095 DAOSGP010000000
VERSION     DAOSGP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17291_length_4512_cov_19.213821"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLV49_03885"
     CDS             complement(<1..251)
                     /locus_tag="PLV49_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOG98018.1"
                     /translation="MQNVDTTKYVIYADITAEGIVERPDVVGAIFGQTEGLLGSDLDL
                     RDLQKTGRLGRIDVQITSSGGKSSGTISIPSSFDKVETA"
     gene            complement(676..1713)
                     /locus_tag="PLV49_03890"
     CDS             complement(676..1713)
                     /locus_tag="PLV49_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="60S ribosomal export protein NMD3"
                     /protein_id="HOG98019.1"
                     /translation="MQSLCPRCGQPAEDGLCPQCRMNSLKLVQFPDVVEVVICSVCGS
                     QQIKGKWQFPDSRSIEDLAAGAAADSVWLHHEFIHPNIEVDLRKVGATRYIAGMRIRG
                     SFMEQMVEETGEVPVRIKLVACERCSRMAGKYFQSTVQLRGNGSRPISSREMEECRRM
                     AQDMADAGYRGGDQLSFIQDMKEVKGGLDIILGSTQLGRRMARAVQERFGGKLLETCK
                     LVGKKENRDVYRSTLLVRFPRLRRGDIVSHRGSLCMVTGFDGKNTLSTSLNEGHRSCM
                     SEEVSGEVRVLGNRADAMKAVVISKDDDVLEIMDPETFRSALASRPRGLEVEPGEEVE
                     VVRTADGFIVL"
     gene            complement(1713..2078)
                     /locus_tag="PLV49_03895"
     CDS             complement(1713..2078)
                     /locus_tag="PLV49_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 family protein"
                     /protein_id="HOG98020.1"
                     /translation="MEMEDSNIQIRRKAADERDEEETVQICLKTYRYGAETLIAVCDC
                     DILGREFREGNLHIEVCSDFYGDEKASLSAVEEALQGATMANLVGCKVVKHAILLGWV
                     DEENVLSIDGVLYAQMVKM"
     gene            complement(2089..4173)
                     /locus_tag="PLV49_03900"
     CDS             complement(2089..4173)
                     /locus_tag="PLV49_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HOG98021.1"
                     /translation="MPADPVAKWEEFLRSRYWDDILKLADSYPDERSLKIQFSDLEKF
                     DPDFAEELMENPDETIEAARTAILELDLPMDVYLDRAHVRIVELPRHFKTRELRSDHI
                     GKLLAIDGLVRTATEVRPKIVSAAFQCQRCGFTFFKEQTGNKFEDQNLKCMNQACDRG
                     GPFKLLLAQSKFVDAQKIRVQESPEDLRGGQQPQTLDVELEDDLAGRIFPGDRVIVNG
                     VLKSYQRTNPQQGKSTYFDLFHKGVSVEMKDQEFEEIDISAEDEEAILEMSRDPEIYE
                     KIKDSIAPSIYGYDDVKEALGLQLVSGFEKHLPDGARIRGDIHILLVGDPGIAKSQLL
                     RYMVKLSPRGIYTSGKSSTSAGLTATAVKDELGDGRWTIEAGALVLADKGIAAIDEMD
                     KMNNEDKSALHEAMEQQTISVAKAGVMATLKSRCSLLAAANPKLGRFDKYEPIAPQIN
                     LTPALMSRFDLIFVLTDDPDTKRDSAIAEHILKSNYAGELSTQKPWNPEISQEDIDNA
                     LTVIEPAIDPEMLRKYVAYARKNVFPTLTEEAKEFFLNYYVGLRTQGQDSNKPVPVTA
                     RQLEALIRLGEASARLRLSREVTGVDAQRVVRILEACLRKVGVDPETGFLDADIIASG
                     TTKSTRDRTKSVMDIIKEVSRENQGPAPREDVLNRAEELGIERSKAEEIIDRMRRDGD
                     VFEPRPGMLKLP"
     gene            complement(4237..>4512)
                     /locus_tag="PLV49_03905"
     CDS             complement(4237..>4512)
                     /locus_tag="PLV49_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOG98022.1"
                     /translation="MALALILPSLILGLFMAFAIYKVAQARFAPMVIDVFEGEEAVAI
                     DRLDPKGYVLFQGEYWLAEAEGTVEKGEKVIIMARGGGQLKVRAGPK"
BASE COUNT          976 a         1349 c         1089 g         1098 t
ORIGIN      
        1 atggcagtct cgaccttatc gaagcttgag ggaattgaaa tggtgccgct cgactttccc
       61 ccgctggatg tgatttgcac atcgattctg cccaaccttc ctgtcttttg aaggtctcgc
      121 aggtcaaggt cgcttccgag gagaccttcc gtctgaccaa agatggcgcc gaccacatct
      181 ggccgctcca ctatcccctc tgcagttata tccgcataaa tgacatattt agtggtatca
      241 acattctgca tgattatatg acccctttta atttttaata gttaaataaa agtcacacca
      301 acatcttaca gccaaagcta ccaataacca aagggggcca tttttcgcga ccaaatgtcg
      361 ccgttccggc catgcctaat ctcaggcagg caatgagcat tgaatgatgg tgaatgtgaa
      421 tttatccaac tacttcagcg tccaattgtc atcatcatgg aacatctgat agaaatgccc
      481 acacatgagc attttcttcc tcgctttgct ggcatttcat cgcctcaagt ttactggagg
      541 cttaattatt atgagatttg aagctcaaat gtgtacttct accaagtaaa attgcacgaa
      601 tctagtttaa gaactttgtg gtagagatga agtgaagagg agaggggcag atcgactcaa
      661 gatatataaa aattcctaca atactataaa tccgtccgct gttcgtacta cctccacctc
      721 ttctccaggc tccacctcca ggcctcgggg acgagacgcg agggcagagc ggaatgtctc
      781 cgggtccatg atctccagga catcatcatc tttggagatg acaactgcct tcatggcatc
      841 agccctgttc cccagcacac gtacctcgcc agagacctcc tcggacatgc aggaccggtg
      901 accttcgttc agggaggtcg acagagtgtt cttgccatca aatcccgtga ccatgcagag
      961 cgagccgcgg tgggatacga tatctcctct tctaagtcgg ggaaacctga ccagcaatgt
     1021 ggagcggtaa acatcgcgat tctccttctt ccccaccagc ttgcatgttt ccagcagctt
     1081 gccgccgaac cgctcctgca ctgccctggc catgcgcctg cccagttggg tcgatcccaa
     1141 aattatgtcc agtccgccct tgacctcttt catatcctgg atgaaggata gctgatcgcc
     1201 tccccgatag cctgcatcgg ccatatcctg agccatcctc cggcactcct ccatctcccg
     1261 ggaggaaatg ggtctgctgc catttcctcg cagctgcact gtggactgga agtacttccc
     1321 tgccatgcgg ctgcagcgct cgcaggccac cagcttgatc ctcacgggga cctcgccggt
     1381 ctcctccacc atctgctcca tgaagcttcc ccggatcctc atcccggcaa tataacgagt
     1441 ggctcccacc tttcggaggt caacctcgat attcggatga atgaactcat ggtgaagcca
     1501 aacagaatca gcagcagctc cagcggccaa atcctctatg gagcggctgt ctgggaactg
     1561 ccactttccc tttatctgct gggaaccaca tacagagcag atcacgacct ccactacatc
     1621 cgggaactgg acaagtttta aagaattcat gcgacactgc ggacagagcc cgtcttctgc
     1681 aggctggccg catctggggc agagggactg catcacattt tcaccatttg ggcatagagg
     1741 actccatcga tggagaggac gttctcctca tccacccagc ccaaaagaat ggcatgcttt
     1801 accactttgc atccaacgag attggccatt gtcgccccct ggagggcttc ctcaaccgcg
     1861 gataagctgg ccttttcatc tccataaaaa tcggaacaaa cctcgatatg caaattgccc
     1921 tctctaaact cacgtccaag tatatcgcaa tcacagacgg caatcagagt ctctgctcca
     1981 tacctatagg tcttgaggca tatctgaaca gtctcttcct catctctctc atccgccgct
     2041 ttcctgcgga tctgtatatt gctatcttcc atttccatct cttcaacctc agggcagctt
     2101 caacatccct ggcctgggct cgaagacatc tccatctctt ctcatccggt cgatgatctc
     2161 ctccgcttta gacctttcta tgcccagctc ctctgctcta ttcagaacat cctctctagg
     2221 cgcagggccc tggttctccc ggctgacctc ctttatgatg tccatcaccg attttgttct
     2281 atcccgggtg ctcttggtgg tacccgaggc gatgatatcc gcatccaaaa agccagtctc
     2341 cggatctacc cccaccttac ggagacaggc ttctaagatt ctgaccacac gctgggcatc
     2401 aactccagtc acctcacggc tcagccgcag cctggcgctt gcctcgccca atcttatgag
     2461 cgcctccagc tgccgggcgg tgaccggaac gggcttgttc gagtcctgcc cctgggtcct
     2521 gaggccgaca taatagttca ggaaaaactc ttttgcctcc tcggtgaggg tgggaaaaac
     2581 gttcttgcgg gcgtaggcca catattttct cagcatctcg ggatcgatcg ccggctcgat
     2641 cacagtgaga gcattatcga tgtcctcctg ggatatctcc gggttccagg gcttttgggt
     2701 ggagagctct ccggcgtaat tgctcttcag gatatgctcg gcgatagcag aatccctctt
     2761 ggtgtccggg tcatcggtga gaacaaagat cagatcgaaa cgggacatga gagcaggggt
     2821 gaggttgatc tgaggggcta tgggctcgta tttatcgaac ctccccagct tcggattggc
     2881 cgcggctaaa agcgagcagc gggacttcag ggtggccatc accccggcct tggcgacgct
     2941 tatcgtctgc tgctccatgg cctcatgcag ggcgctcttg tcctcgttgt tcatcttgtc
     3001 catctcatcg atagcggcga tccccttatc tgccaggacc agggccccgg cctctatggt
     3061 ccagcgaccg tcacccagct cgtccttgac cgcagtggca gtaaggccgg cagaggtaga
     3121 gctctttccc gaggtgtaaa tcccccgggg ggagagcttg accatatacc tcaagagctg
     3181 cgattttgcg atgcctggat cgcccaccag caggatgtga atgtctcctc tgatgcgcgc
     3241 tccgtccggg agatgcttct cgaatcccga taccagctga agtcccaggg cctctttgac
     3301 atcatcatag ccgtatatgg agggggcaat ggagtccttg atcttctcat agatctcggg
     3361 atcgcgagac atctccagga tcgcctcctc gtcctcagca gagatatcta tctcctcgaa
     3421 ctcctggtct ttcatctcca ccgatacccc tttatggaat aaatcgaagt aggtgctctt
     3481 tccctgctgg ggattggtcc tctgatagga cttcaaaaca ccattcacaa tcaccctatc
     3541 gccaggaaag atccggcccg cgaggtcgtc ctccaattcg acatccaggg tctggggctg
     3601 ctggccgccg cgcaggtcct cgggcgactc ctgcactctg atcttctggg catcgacgaa
     3661 cttggactgg gcgagcaaga gcttgaaggg gccgccccga tcgcaggcct ggttcatgca
     3721 cttcaggttc tggtcctcga atttgtttcc tgtctgctct ttaaagaagg taaagccgca
     3781 cctttggcac tggaatgcgg cggagactat cttcggcctg acctcggttg ccgtcctcac
     3841 cagtccgtct atggccagga gcttgcctat atggtccgag cgaagctcgc gggtcttgaa
     3901 atgccttgga agctcgacta ttcgcacatg ggctcgatcc aaatagacgt ccatgggcag
     3961 atccagctcc aggatggcgg tccgggcagc ctctatggtc tcatccggat tctccataag
     4021 ctcctctgcg aaatccggat cgaacttttc caggtctgag aactggattt tgaggctgcg
     4081 ctcgtccgga taggagtcag ctagctttaa gatatcatcc caatatcggg accggaggaa
     4141 ctcctcccat ttggcaacag gatctgcggg cattctgaga cgagttacgc tggccaggta
     4201 tctaaagcat ttgggggaat actggaagag ggcagcttac ttcggcccgg ctctaacctt
     4261 cagctggccc cctcccctgg ccattataat caccttctct cccttctcca ccgtcccttc
     4321 cgcctctgcc agccagtatt ctccctgaaa gagaacatag cccttgggat ccagccggtc
     4381 tatggccact gcctcctctc cctcgaagac atcgatcacc ataggagcaa aacgggcttg
     4441 agccaccttg taaatggcaa aggccatgaa caatcccaat atgagcgagg gcaggatcag
     4501 ggccagggcc at
//
LOCUS       DAOSGP010000096         4490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_17428_length_4490_cov_20.245321, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000096 DAOSGP010000000
VERSION     DAOSGP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17428_length_4490_cov_20.245321"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PLV49_03910"
     CDS             complement(<1..1442)
                     /locus_tag="PLV49_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOG98023.1"
                     /translation="MIGQNTALVVMVLYLLLLFAIARFSDNRKLEGKSIVSNPYVYAL
                     SLSVYVSAWTFYGSIGRAASTGLEFLPIYLGPILAMTLGWVLIRKMIRVSKEYRLTSI
                     SDFLSFRYGRSYAIGAVVALLSLVMVTPYVALQLIAISTSLETLAESHTLLSFSWEST
                     LVVALLLGIFAMIFGARQLDPMERHEGLIAVVAFESLVKLVAFLMIGLFVAYGIFGGY
                     GDIFSQMAEQARSNPNYSNLMDVEYPLWFSLTLVSFFAILFLPRQFHVMVVENSEVGH
                     LKKAMWLFPLYLLLINLFVPAIAWGGLLLNTPGLKDSFIITIPYSHNQNLLSLLVFIG
                     GTSAATAMILVESVAVGTMLLNDLELPYILRHIKKQKNLANALLNTRRLNILLVVLLG
                     YLYSRVAAYQILADIGIASFLAASQLAPAALGGLYWKGGNRKGAMAGLVSGFLLWSYT
                     ALIPTIAEDEGMLSSLVRDGAFGLAFLRPT"
     gene            1793..>2994
                     /gene="aspS"
                     /locus_tag="PLV49_03915"
     CDS             1793..>2994
                     /gene="aspS"
                     /locus_tag="PLV49_03915"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOG98024.1"
                     /translation="MDGKEVMLAGFAHEIRDLGGIAFLVLRDREGMAQITLVKKLLGK
                     DVFKMARSISRESVVMVRGNVKAEAKAPRGYEILPTEIQVLSAAQVPLPLDPTEKVEC
                     ELDTRLDSRFMDIRRPCVLAAFEIESAVLCSLRDYFHKKGIVEVFTPKIVAAATEGGT
                     DLFPISYFEKEAFLNQSPQLYKQMLMGAGMDRVYEIGPIFRAEEHDTRRHLNEAISID
                     LEVSFADDKDVMEILEEAVAGAYSYVADNCRRQLEVLNMELEVPRLPFKRITYTRALE
                     LAEEKAGARMQWGDDLDTETERFLGESIGEHYFLIDWPSSIKPYYTLPYEDKPEICRG
                     FDLMHPRMELASGAQRVHDYSLLVERIKEKGLDPDGFEFYLRAFRYGMPPHAGWGLGL
                     SRLVMTML"
     assembly_gap    2995..3094
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3095..4326)
                     /locus_tag="PLV49_03920"
     CDS             complement(<3095..4326)
                     /locus_tag="PLV49_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOG98025.1"
                     /translation="MINQLLLLAYVLAIILLSLKLRQGTFSGFVLSNRNIAYPAVIGI
                     AYTAAYFSAASFLGGGGYGLAAGMPWVIFCSLFHVGFACIAWIMAGRIWTMAKQYDAK
                     TVPQLLERRYNSPLGKVILAIIMLILYTVYLVPIFKGCATLFQGMIGVSYLEGLLITV
                     AIVAIYYAIGGLPAIIWVGFIQGVLMLIGAVFLYGGLISAGGGLDIWERIPLDVLSMN
                     GLNIPWQQTFGQAFSISLGLLALPDLLIMIFSARDKRVVRFAGIYGPISIAIYSVCIF
                     SLGILAYGALSAEQIAPFLKNPDGLVPFVATLILPKGFDGIILLAAISAAMSTMSAIV
                     LVTATSLTSDILRYLNPGIADRKVLLLTRMIGVAILIIAAVSAINVPQQIVPLVAVSM
                     GIIACCVLVPLFLGIFWQ"
BASE COUNT         1123 a         1064 c         1291 g          912 t
ORIGIN      
        1 tctgtgggtc tgaggaaagc caggccgaag gctccatctc taaccaggct tgagagcatg
       61 ccctcgtcct cggctatggt gggtatcaag gcggtgtagg accagagcag aaatcctgaa
      121 acgagacctg ccatagctcc ttttcggttt cctcctttcc aataaaggcc tccgagagca
      181 gctggcgcca gctgactggc agcgagaaat gaggcgattc cgatatctgc caggatctga
      241 taggcagcaa cacgggagta gaggtatccc aaaaggacaa ccaggagtat attcagcctt
      301 ctggtattga ggagtgcatt ggccaggttc ttctgctttt taatatggcg caggatatag
      361 gggagctcca ggtcgttgag cagcatcgtg ccgacggcca cggactcgac aagaatcatg
      421 gcagtggctg ccgatgtccc tcctatgaag accaaaagag atagaaggtt ttgattgtgg
      481 ctataaggta tggttattat gaaggagtcc ttgaggccgg gggtattgag gagcaacccg
      541 ccccaggcga tggccggaac gaaaaggttg atcagcagga ggtagagggg aaagagccac
      601 atcgcctttt tcagatggcc cacttctgag ttctccacaa ccatgacatg aaactgtctg
      661 ggcaagaata atatggcaaa aaaggacacg agggtcagag agaaccaaag aggatactcc
      721 acgtccatga ggttggaata attcggatta ctacgagcct gctcagccat ctggctgaag
      781 atatccccat agcctccaaa gattccatag gcaacgaata gccctatcat cagaaaagcc
      841 accagcttca ccagggattc aaaggccacc acagcaatca gaccctcatg ccgttccatg
      901 gggtctagct ggcgggcgcc gaagatcatg gcgaatatac ccaggaggag ggccaccacc
      961 agggtgctct cccaggagaa gctcaggagg gtatgggatt cggccagagt ctctaatgag
     1021 gtggagatgg ctatcagctg caaagccaca taaggggtta ccatgaccag gctgagcaga
     1081 gcaaccactg ccccgatggc atagctcctg ccgtagcgaa agctcaagaa atcgctgatg
     1141 gaggtgaggc ggtactcctt ggatacgcga atcatctttc tgatgagcac ccagcccagt
     1201 gtcatcgcca ggataggccc taaatatata ggcaaaaatt ccaggccggt agatgcagct
     1261 cttcctatgg agccgtaaaa ggtccatgcg gagacatata cggataggga gagggcatat
     1321 acatagggat tgctgacgat gctcttgccc tctagctttc tattgtctga gaacctggct
     1381 attgcgaaaa gaaggagcag gtagagcacc ataactacaa gagcggtatt ctggcctatc
     1441 aattagaatt cccccggtca aagaggtaca tcaggagaat gagtatcaac cagactgcag
     1501 atatgtagat gagtattgca ggcatgccca gcagaattat gccatcggct aaggatagca
     1561 taggccagtt gagcagcacc catcctaaga agaagagcaa aatccagaaa tccttctggc
     1621 tcaggagttc gtggagtgag gccatggcta taaacccgga taggttatca tccccttagc
     1681 agatatatct actgcatgta gatcagactg cttttatatc aggaattcgt tctcgcagtc
     1741 gtgagatgaa ttgatcctca gaactcatta ttcaggccaa atatctgcgg atatggacgg
     1801 aaaagaggtc atgctggcgg gcttcgccca tgagatcagg gacctgggag gcatagcttt
     1861 cctggtgctc agggacagag agggaatggc tcagataact ctggtcaaga agctcctggg
     1921 aaaggatgtt ttcaagatgg cgcgctccat cagccgggag agcgtggtta tggtccgggg
     1981 gaatgttaag gcggaggcca aggctccgcg aggatatgag atccttccca cggagattca
     2041 ggttttgagc gccgcccagg tgcctcttcc tttggatccc acggagaagg tggagtgcga
     2101 gctggacacc cgccttgact ccaggttcat ggacatccgc cggccctgcg tcctggccgc
     2161 atttgagatc gagagcgcag tcctttgcag cctcagggac tatttccata agaaggggat
     2221 agttgaggtc ttcaccccca agatcgtggc cgcagccaca gagggaggga ctgatctctt
     2281 tcccatcagc tactttgaga aggaggcctt cttgaaccag agcccacagc tctacaaaca
     2341 gatgctgatg ggagcgggca tggaccgtgt ttatgagatc ggtcccatat tccgggcgga
     2401 ggagcatgat accaggcgcc atctgaatga ggccatctcc atagatctgg aggtgagctt
     2461 tgccgatgac aaggatgtaa tggagatcct ggaggaggcg gtggccgggg catatagcta
     2521 tgtggctgat aactgccgcc gccagctgga ggtcttgaat atggagctgg aggtgccccg
     2581 gcttcccttc aagaggatca cctacacccg tgctctggag ctggcggaag agaaggctgg
     2641 ggccaggatg caatggggcg acgacctgga tacagagaca gagagattct taggagagag
     2701 cattggagag cattacttcc tgatagattg gccatcatca atcaagccct actatactct
     2761 gccttatgag gataagcctg agatctgccg gggattcgat ctcatgcacc cgaggatgga
     2821 gctggccagc ggggcgcaga gggtgcacga ctacagcctg ctggtggaga ggataaagga
     2881 gaaggggctg gatcctgatg gctttgaatt ctatcttcgg gcttttcgct atggaatgcc
     2941 ccctcatgcc ggttggggtc tggggctgtc ccgcctggtg atgaccatgc tcaannnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctctgc cagaagatgc ccaagaacag
     3121 tggcaccagg acgcagcagg cgatgatacc catgctgacc gccaccaggg gcacgatctg
     3181 ttgtggcaca ttgatcgccg agacggcggc gatgatcagt atagccaccc caatcatgcg
     3241 ggtgagtagg agaaccttcc ggtcggcaat tcctggattc agatagcgca ggatgtccga
     3301 ggtgagggag gtggcagtga ccagcacaat ggcgctcata gtggacatgg ctgcagatat
     3361 ggctgccaac aggatgatcc cgtcaaagcc cttgggcagg atcagtgtgg cgacgaatgg
     3421 caccaggccg tcgggattct tgaggaatgg agctatctgc tcggcgctca atgctccata
     3481 ggccaggatt cccagggaga agatgcatac tgaatagatg gcgatggaga tgggtccgta
     3541 aatgcccgcg aagcgaacga ccctcttgtc ccgggcggag aatatcataa tcagcagatc
     3601 gggaagggcg agcagtccca ggctgatgga gaaggcctgt ccaaatgtct gctgccaggg
     3661 gatattaagg ccattcatgc tgagcacatc aaggggaatt ctctcccaga tatccagtcc
     3721 gccgccagcg gagatcaggc cgccgtagag gaagacggcc cctatcagca tcaatacgcc
     3781 ctggatgaat cccacccaga ttatcgccgg cagcccgccg atggcgtagt aaatggccac
     3841 tatggctacg gtgatcaata gaccctccag gtaggatacg ccaatcattc cctgaaatag
     3901 ggtagcgcat cctttgaaga tggggaccag gtatacggta tagaggatga gcatgatgat
     3961 agcaagtatg acctttccca ggggagagtt gtatcttctc tccagcagct ggggaaccgt
     4021 cttggcatcg tactgctttg ccatggtcca gatcctcccc gccatgatcc aggcgatgca
     4081 ggcgaatccg acatgaaata gggagcagaa gatcacccag ggcatgccgg cggcaagtcc
     4141 gtaaccgcct cctcccagga aggaggctgc gctgaagtag gcagcagtat aggcaatgcc
     4201 gatgacggca ggataggcga tgttgcggtt ggagaggaca aagcctgaga atgtcccctg
     4261 tcgcagctta agtgagagca ggattatcgc caggacgtag gccaacagca gcaattgatt
     4321 aatcatggta tttcctcatt aatataaata tagttgtgga tatcataact ataagagcga
     4381 tcagtagcgc aatgtagggg gtgggcatgc tcattttttc tcaccctgaa gtagtcgatc
     4441 acagagcagg agaaatggct gatctgtgtc cctccatatc gaattcatgt
//
LOCUS       DAOSGP010000097         4449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_17679_length_4449_cov_17.866636, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000097 DAOSGP010000000
VERSION     DAOSGP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17679_length_4449_cov_17.866636"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..1486)
                     /gene="thiC"
                     /locus_tag="PLV49_03925"
     CDS             complement(197..1486)
                     /gene="thiC"
                     /locus_tag="PLV49_03925"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOG98026.1"
                     /translation="MTLMEEASSGRVPQSIERVARKEGIDPAKLARLVAAGRVVIPGN
                     VRREGLDPAGIGEMLAVKVNVNLGTSQNLDSPEVEVEKALAAAEAGADAVMDLSTGGD
                     LDLVRKKILAAVKVPLGTVPIYQAAVKKELTSQGMFDALERHARDGVDFVTVHAGVNK
                     ISLERLRQDPRLIGVVSRGGSLTMKYIADTGEENPYYQEFDYLLEIAREYELTLSLGD
                     GLRPGCIEDASDRAKYMEFIMLGELVKRAQAAGVQAMVEGPGHVPADEIETSVRAMKH
                     LTGGAPLYLLGPLVTDVAPGYDHITAAMGGVIAGMAGADFLCATTPSEHLDLPNREDV
                     IEGTYVTRIAAHAADLTRPGVRERARSWDLQMSRARAALDWDGQFQAAINPARAKQIR
                     HRRGLETDTCTMCSELCAIRLAKEAMEKERDKDPKCA"
     gene            1540..>1895
                     /locus_tag="PLV49_03930"
     CDS             1540..>1895
                     /locus_tag="PLV49_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG98027.1"
                     /translation="MVEVHKVSGAAFDGNVYLVLDEKPILVDAGMMAGPTLKNIKKFI
                     DPAKIELIVLTHCHHDHSGAAPALKEATGARLMLSEKEVGCIGDELASVAYLFGQQAP
                     EYKVDETLKEGMVLDIG"
     assembly_gap    1896..1995
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1996..2333
                     /locus_tag="PLV49_03935"
     CDS             <1996..2333
                     /locus_tag="PLV49_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG98028.1"
                     /translation="PEYKVDETLKEGMVLDIGKWKLEVMETPGHSTGSLCLYERTEKV
                     LFSGDTVFPDGNIGRTDMFGGSTDELVRSIQRLTELDVKVMYPGHMDITSRDVNRQIQ
                     MSLRFAKSI"
     gene            2353..3501
                     /locus_tag="PLV49_03940"
     CDS             2353..3501
                     /locus_tag="PLV49_03940"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HOG98029.1"
                     /translation="MTDKKDLVKRESESIFQTYGRQDVLLVKGSGAHVWDSEDREYLD
                     FVAGIAVNSIGHCHPKLVEAVSRQAAALIHTSNLYYTENQVLLAEELKGLTGMDRAYF
                     CNSGAESIEAALKLVRRSTGRSKIVAAERAFHGRTLGSLGLTHKAAYREPFRPLREAE
                     FVPYNDSEALKAAVNSETGAVFLEPVQGEAGVYVPDGDYIRTARDICDDQGALLILDE
                     VQTGFGRTGRWFAKEHSGVVPDVICLAKAIAGGLPMGAMLARGSSSEAFRKGDHGSTF
                     AGGPLICAAALATIDVIREEKLVERSEELGRYLKSEIEKLEAQEVRGLGLMVGLDLNC
                     DCKSLVEKALQKGVLINNTGEHTLRLIPPLVVTKEEIDRVVEVIGELL"
     gene            3488..>4449
                     /locus_tag="PLV49_03945"
     CDS             3488..>4449
                     /locus_tag="PLV49_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG98030.1"
                     /translation="MSSSRQSTRESLKRLRPYVAGKGFTEISRRYGLDPTDIVKLGSN
                     ENPYGPSPKVFEALQNVSPERYPEPEELMEGLARYTGSPEEMLVIGAGMDGVMDTLTR
                     LFLEKGDRTFIPTPTFSYYEILTVLAEAEPLFSPRGPDFRIYERIADEAKMAFICSPN
                     NPTGNALKEEELCGLLETTEAIVFLDEAYVEFAERSLAELVSRYENLVVGRTLSKAFG
                     LAGMRLGYALMPEWMAREFCRAAPPFFGISCASVAAGVAALSDLDFMNESVRKIRMER
                     SRLQKEIRSYPSQANFLYLKTEEASDSFAEKFLQKGIIIRDCRS"
BASE COUNT          931 a         1246 c         1290 g          882 t
ORIGIN      
        1 tatccgttcg cgccatcaac accgtggaag gggggaaggc caccgccacc tccaacataa
       61 tcatcgagcc ggtgcagatc atccagctcc cttgccaagc agcagataag ctgagatgat
      121 caagggccgc ccaaaaatta tgggagaggg gttcagaaga tcacaacagt gtcccgtcca
      181 gccaggacgg gctgtatcag gcacacttcg gatccttatc cctctctttc tccatcgcct
      241 cttttgccag ccggatggca cacagctcag agcacatggt gcaggtgtcg gtctctaatc
      301 ctcgcctgtg tctgatctgc ttagctcttg ccggatttat cgccgcctgg aactggccat
      361 cccagtccag agcggctcgc gcccgggaca tctgcagatc ccaggatcga gctctttctc
      421 gcacgcccgg ccgggtgagg tcggctgcat gggcagcgat ccgagtgaca taggttccct
      481 cgattacgtc ctctctgttg ggcaggtcga ggtgctcgga gggggttgtg gcacagagga
      541 agtccgctcc cgccatgccg gcgatcacgc cacccatggc agcggtgata tgatcgtacc
      601 cgggggcgac gtccgtcacc aggggtccga gaaggtataa gggcgctccg ccggtcaaat
      661 gcttcatagc cctcacactg gtctcgatct catccgcggg cacatgcccc ggcccctcca
      721 ccatggcctg aactcccgca gcttgcgccc ttttaaccag ctcaccgagc atgatgaact
      781 ccatgtactt ggcccggtca gatgcatcct cgatgcaccc tggccgcaga ccgtcgccca
      841 gtgagagggt caattcatat tccctggcga tctccagcag gtagtcgaac tcctggtagt
      901 agggattctc ctctccagta tcggcgatat atttcatggt caaagagccg ccccggctca
      961 ccacccctat cagtcgaggg tcctgcctga gccgctccag ggatatcttg ttcaccccgg
     1021 catgaacggt gacgaagtcc accccatctc gggcatgtcg ctccagggca tcaaacatcc
     1081 cctggctggt cagctccttt ttgacggcgg cctgatagat gggcactgtg cccagaggca
     1141 cttttactgc tgccaagatc ttcttgcgca ccaggtccag gtcacctccg gtggacaggt
     1201 ccatcacagc atccgctccc gcctcagctg ccgccagagc tttttccacc tctacctccg
     1261 gggagtcgag gttctgggat gtgcccaggt tgacattcac tttaacggct agcatctccc
     1321 ctatgcctgc cggatccagc ccctcgcggc ggacatttcc cgggatgacc accctgcccg
     1381 cggccaccag ccgggccagc tttgccggat ctattccctc ttttcttgcc acccgttcga
     1441 tactttgggg cacgcgccca ctgcttgcct cttccatcag ggtcattgtt accaactaat
     1501 atattgtagg agcaactttc tcgctgcagg tgtttatcaa tggttgaagt gcataaggtt
     1561 agcggtgccg ccttcgatgg caatgtctac ctggtcttgg atgagaagcc cattctggtg
     1621 gacgcgggca tgatggcagg gccgacctta aagaacatca agaaattcat cgatccagcc
     1681 aagatcgagc ttatcgtcct cactcactgc catcacgatc actccggtgc cgctcccgcc
     1741 cttaaggagg cgacaggagc acgcctgatg ctgagcgaga aggaggtggg ctgcatagga
     1801 gatgagctgg catcggttgc atacctcttc ggccagcagg ccccggagta caaagtggac
     1861 gagaccctga aagaggggat ggtcctggat attggnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnccccg gagtacaaag tggacgagac cctgaaagag gggatggtcc
     2041 tggatattgg gaaatggaag ctggaggtca tggagacccc agggcactcc accggaagcc
     2101 tgtgccttta tgagcggacg gaaaaggtgc tattctccgg agatacagta ttccctgacg
     2161 ggaacatcgg ccggacggat atgttcggcg gaagcaccga cgaattggtc cgctccatcc
     2221 agaggctgac agagctggat gtcaaggtta tgtatcccgg ccatatggat ataaccagcc
     2281 gagatgtcaa tcgccagatt caaatgagcc tgaggtttgc caagagcatc tagtgggatg
     2341 ggtgtcaatc tcatgacaga caagaaagat ctagtgaaaa gggaatccga atccatattc
     2401 cagacctacg gccgccagga cgtgctcctc gtcaagggaa gcggtgctca tgtttgggac
     2461 agcgaagaca gggaatacct ggacttcgtc gctggcatag cggtgaacag cataggccac
     2521 tgccatccta agttggtgga ggcggtcagc cgccaggctg cagctctcat ccacacctcc
     2581 aacctctact acaccgaaaa ccaggtactt ttagcggaag agctgaaggg gctcaccggc
     2641 atggaccggg catacttctg caactctgga gcggagagca tagaagcggc gctcaagctc
     2701 gtccgccggt caacgggcag gtcgaagatc gtggcggctg agagggcctt tcacggacgc
     2761 accctggggt cccttggcct aactcataaa gccgcttata gggagccgtt ccggccgctg
     2821 agggaagcgg agttcgtccc ctacaacgat agcgaggctc tgaaggcggc agtcaactcc
     2881 gagaccggag cggtgttcct ggagccggta cagggtgagg caggggtcta tgtgccggac
     2941 ggggattata tcagaacagc gcgagacata tgcgatgacc aaggagccct tctcatcctg
     3001 gatgaggtgc agaccggatt tggacggacc ggcaggtggt ttgccaagga gcactccggg
     3061 gttgtccctg atgtcatctg cctggccaag gccatagccg gaggcctgcc catgggagcg
     3121 atgctcgccc ggggcagctc ttctgaggcc ttccggaaag gagaccacgg ctcgacattc
     3181 gccggcggcc ccctgatctg tgctgccgcc ctggcaacga tcgatgtcat acgagaggag
     3241 aagctggtcg agcgctcaga ggagctgggc agatatctca aatcggagat agagaagctc
     3301 gaggctcaag aggttcgcgg cctggggctg atggtcggcc tggatctgaa ttgcgactgc
     3361 aaatcgctgg tggagaaggc tctccaaaag ggagtgctga tcaacaacac tggagagcat
     3421 accctccgcc tcatcccgcc cctggtggtg acgaaggagg agatagacag agtggtggag
     3481 gtaataggtg agctcctcta ggcaaagtac cagggagagc ctgaagaggc tgcgccccta
     3541 tgtggcaggc aagggtttca ctgagatctc ccgccgctat ggtctcgacc cgacggatat
     3601 cgtaaagctg ggaagcaatg agaatccata cggtcccagc cctaaggttt ttgaggccct
     3661 gcagaatgtc tccccggaga ggtatccgga gcccgaggag ctgatggaag ggctggccag
     3721 atacaccggc tctcccgagg agatgctggt cataggagcg ggcatggacg gggtgatgga
     3781 caccctgacc cggctctttt tggagaaggg cgaccgaacc ttcatcccca cccccacatt
     3841 cagctactat gagatcctga ccgttctggc ggaggctgaa cccctattct caccccgggg
     3901 cccggatttc cggatttatg agagaattgc agatgaggca aagatggctt tcatctgctc
     3961 gccaaacaat cccaccggaa acgccctgaa agaagaggag ctttgcggtc tgctggagac
     4021 gacggaggcc atcgtctttt tggacgaggc ctatgtggag tttgcagagc ggagcctggc
     4081 ggagctggtg agtagatacg agaacctggt ggtgggacgg actttatcca aggccttcgg
     4141 cctggcgggg atgcgcctgg gctatgccct gatgcccgag tggatggctc gagagttctg
     4201 ccgggcggcg ccgcccttct ttggcatatc ctgcgcctca gttgcggctg gtgtggcagc
     4261 tctttcggat ctggacttta tgaacgaatc ggtgagaaag ataagaatgg agcgcagtcg
     4321 cctgcaaaaa gagatcaggt cttatccctc ccaggccaac ttcctctacc tgaaaaccga
     4381 ggaagcctcg gactcatttg cagagaagtt cctgcaaaaa ggtatcatca tccgcgactg
     4441 ccgctcatt
//
LOCUS       DAOSGP010000098         4361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_18163_length_4361_cov_18.429633, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000098 DAOSGP010000000
VERSION     DAOSGP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18163_length_4361_cov_18.429633"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PLV49_03950"
     CDS             complement(<1..1226)
                     /locus_tag="PLV49_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOG98031.1"
                     /translation="MTTDEVLSKLDSDQKGISSTQAKERLSQHGPNQLESTTKPSPLK
                     IFIGKFKDYMVLVLIFAAIISFIAGETTNAYVILGIVVLVAIIGFVQEYKAEKAMEAL
                     REMVAPEADVIRDGQMSTIPAADLVPGDVVFLEAGDKVPADGRILEVTSLQVIESSLT
                     GESMPVEKLAQTLPEDIALADRKNMVFMGTIISQGNCRAIVTATGLGTELGRISGLMK
                     QEQAEPPLKIKLQQLAKRQAFLVMVISAIVFILMFSRGLPVIDALIASIALAVAGIPE
                     ALPFIVTLALAFGTQAMAKKNAIIRRLPEVETLGSTTVICTDKTGTLTTGEMTLREIR
                     TYRKIEVTGAGYDPTGQFISNGAKLEPTEEDLARILKIGVHANNSAIERANGGWRVVG
                     DPTEGALIVAAKKAGIL"
     assembly_gap    1467..1566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1567..2328
                     /locus_tag="PLV49_03955"
     CDS             <1567..2328
                     /locus_tag="PLV49_03955"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOG98032.1"
                     /translation="LIDNMREIMLEMGFVEIKGPIVQTSFWNFDALFQPQDHPAREMQ
                     DTFYLNSQGDIPDYARIKEMHECGGSVGSTGWGGCWSPEVARQEVLRTHTTGVTIKYL
                     AEHPRPPVKAFGIDRVYRREAIDSTHTPEFEQLEGIVMDESVSFCHLLGILKEFYGKM
                     GFEEVRFRPGYFPYTEPSVEPEVYIDGLGWVELGGAGVFRKEVTAPFGIDHPVLAWGL
                     GVSRVAMLRLGLKDLRLLYQSDIEWLRQTPVSIKL"
     gene            2339..3985
                     /gene="pheT"
                     /locus_tag="PLV49_03960"
     CDS             2339..3985
                     /gene="pheT"
                     /locus_tag="PLV49_03960"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG98033.1"
                     /translation="MPVVRLYYQDMERLIGASRETIMSRLAMMGADIGKRAEEEYVDV
                     EFFPDRPDLYSSEGVARAMQGFLGIKTGLVSYSVSPGPVVVQVEESIKSVRPLIGCAV
                     VRGLEFTDEAIESLMGLQEDLHWGLGRNRRKVAIGVHDISRVRPPFRYFGESPQRRFV
                     PLDYSEEMTMEEILHDHPKGKDYGHILKDCPVYPLIVDADDRVLSFPPIINGELTSVT
                     EETEDLFIDVTGTDPVVHKALNIVVTSLAERGGKIESVLVKRSEGDFLSPDLSPASWK
                     VRTEEANRLIGFDLTGAELAECLKRMRFGAVTAGEEMDDIVVVQVPAYRADIMHSWDI
                     FEDAAKAYGYDNLEARLPQTVTVGRAHSSEVRKGEIREIMAGLGYLETMPFTLTNEKV
                     HFQWMRRFYAPEVAGAVRVLHPISELHTILRTSLLSSLLEIFALNQHHPLPQRIFAAG
                     DVVKDKKTRMNLAAASIHSGANYSEIRSVVDAVLREMDLEADIAPTKDRAFMPGRGAD
                     LFAEGKRIGCFGELHPLLLRSFGLEQPAVGLEIEWGELEQ"
     gene            4174..>4361
                     /locus_tag="PLV49_03965"
     CDS             4174..>4361
                     /locus_tag="PLV49_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG98034.1"
                     /translation="MKVRDLMSYPIVTVPPEAMVLDAIKVMAAQKKGSVLVAKERLLK
                     ECLGIVTTSQIFLEVFARG"
BASE COUNT          911 a         1120 c         1272 g          958 t
ORIGIN      
        1 agaatccctg cttttttggc ggccacgata agcgctccct ccgtaggatc gcccaccacc
       61 ctccagccgc cattggccct ctctatggcg gagttgttgg catgcactcc aatcttgaga
      121 atcctcgcca ggtcctcttc tgttggctcg agcttcgccc cattggatat gaactggccg
      181 gtgggatcgt atcctgcccc cgtgacctcg atctttctgt aggtgcggat ctcacgaagg
      241 gtcatctctc ctgtggtcag agtaccggtc ttatcggtgc agatcaccgt ggtcgagccc
      301 aaggtctcca cttcgggaag ccttctgatg atggcgttct tctttgccat ggcctgggtt
      361 ccaaaggcca gtgccagtgt gactatgaag ggaagggctt cagggatgcc ggctactgcc
      421 agagcgatgg aggcgatgag ggcatctatg accggcaacc cccggctgaa catgaggatg
      481 aagacgatcg ccgatatcac cataaccagg aaggcctgcc ttttcgccag ctgctggagc
      541 ttgatcttga gaggcggctc ggcctgctcc tgcttcatca ggcctgagat ccttcctagc
      601 tctgtgccga gaccggtggc ggtgactatt gcccgacagt tgccctgaga tatgatggtc
      661 cccatgaaga ccatattctt cctgtctgcc agcgctatat cctcgggcag ggtctgggcc
      721 aacttttcca ccggcatgga ctctccggtt aaagaggact cgatgacctg aagggaggtc
      781 acctccagga tgcggccgtc ggctggcacc ttatcccccg cctccaggaa gaccacgtcc
      841 cctggaacaa ggtctgcagc aggtatggtg ctcatctgac cgtctctgat cacatccgcc
      901 tctggggcca ccatctccct cagcgcctcc atggccttct ctgctttgta ctcctggacg
      961 aagccgatga tggcaactaa aacgacgatc cccaaaatga cataggcatt ggtggtctct
     1021 ccggcaataa aagagatgat agcggcaaag atcagcacca ggaccatgta gtctttgaac
     1081 ttgcctatga agattttcaa tggcgagggt ttggtagtgc tttcaagctg attggggccg
     1141 tgctgggaga gcctttcttt tgcctgcgtc gagcttatgc ctttttggtc ggaatccagc
     1201 ttggataaga cctcatccgt tgtcatcgtg tgccagttca cagtttttca gacccctatc
     1261 gcattgggat aaagtggagt ccgtcaattg tagaaatact taacgctgat tcctatgaaa
     1321 tcctgaggct attcccttca tcttgccttc cttcactggg gacgctgttc gacaggttta
     1381 tgtatcatca gtctaaccga aaaaaacgtg cctcagaata tagcaaagat cccaattatc
     1441 aatcacagaa ccatgctctc aaagcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnctga tcgataacat gcgggagatc atgctggaga tgggatttgt cgagatcaag
     1621 gggccgatcg ttcagaccag cttctggaac ttcgatgccc tcttccagcc ccaggaccat
     1681 ccggccaggg agatgcagga caccttctat ctgaacagcc agggggatat ccccgattac
     1741 gcccggatca aagagatgca tgaatgcggc ggcagcgtag gatctaccgg atggggaggc
     1801 tgctggagtc cggaggtggc ccggcaggag gtattgcgta cccataccac cggagtgacg
     1861 atcaaatacc tggcagagca tccccggccg ccggtcaagg ccttcggaat cgatcgggtc
     1921 taccggcggg aggctataga ttctacccat accccggagt tcgagcagct ggaggggata
     1981 gtgatggacg agagcgtgag cttctgccat ctcctgggca tcttgaagga gttttatgga
     2041 aagatgggat ttgaggaggt gcgcttccgc ccgggctact tcccctacac tgagccttca
     2101 gtcgagcctg aggtctatat cgacggcctg ggatgggtgg agctgggagg tgccggtgtg
     2161 ttcagaaagg aggttacagc cccattcggc atcgatcatc ccgttttggc ctggggcctg
     2221 ggagtctcca gggtggccat gctaaggctg gggctgaagg acctccggct gctctatcaa
     2281 tcggatatcg aatggctgcg ccagacgccg gtttccatca agctgtgagg agtataccat
     2341 gcccgttgtc agactttatt atcaggatat ggagaggctg attggcgcct ctcgagagac
     2401 gatcatgagc aggctggcca tgatgggtgc agacatagga aagagagcgg aagaggagta
     2461 tgtggatgtg gagttcttcc ccgaccggcc ggacctctat agctcggagg gggtggccag
     2521 agcgatgcaa ggattcctgg ggatcaagac cggcctggtc agctacagcg tctcccccgg
     2581 tcctgtcgtc gttcaggtgg aggaatcaat aaaatcggtg cgccctctga tcggctgtgc
     2641 agtggtgcgg ggtctcgagt tcaccgatga ggccatcgag tccctgatgg gtctgcaaga
     2701 ggatctgcac tggggcctgg gcaggaaccg gcggaaggtg gccataggag tgcacgacat
     2761 atccagggta cggccaccct tccgctactt cggcgagagc cctcaacgca ggtttgtccc
     2821 tctggactat agcgaggaga tgaccatgga ggagatcctg catgatcacc ccaaaggaaa
     2881 ggattacggc catatactga aggactgtcc ggtctatcca ctgatcgtgg atgcagatga
     2941 tcgggtttta tccttcccgc cgatcatcaa tggcgaattg accagcgtca ccgaggagac
     3001 ggaggatctc ttcatcgatg tcaccgggac cgaccctgtg gtccacaagg ccctgaacat
     3061 cgtggtcacc tctctggcgg agaggggagg gaaaatagag agcgttctgg tcaagaggag
     3121 cgagggcgac tttctcagcc cggatctgtc gcccgccagc tggaaggtga gaactgagga
     3181 ggccaaccgg ctgataggat ttgatctgac gggagcagag ttggctgaat gcctgaagag
     3241 gatgcgcttt ggagccgtta ccgccggcga ggagatggat gatatcgtgg tggtgcaggt
     3301 cccggcctac cgggcggata taatgcactc ctgggacatc ttcgaggatg cggccaaggc
     3361 atatggctac gacaatctgg aagcccggct tccccagacg gtgaccgtgg gcagggccca
     3421 ttcttctgaa gtgcgcaagg gagagatcag ggagatcatg gcgggtctgg ggtatctgga
     3481 gaccatgccc ttcaccctga ccaatgagaa ggtgcacttt cagtggatgc gcaggttcta
     3541 tgctccggag gtggcgggcg ctgtgcgggt gctgcacccc attagcgagc tgcataccat
     3601 cctccgtacc tcgcttctca gctcccttct ggagatcttc gccctcaatc agcatcatcc
     3661 tcttccccag aggatattcg ctgccgggga tgtggtgaaa gacaagaaga ccaggatgaa
     3721 tctggctgct gcatccattc acagcggggc gaattattcg gagatcaggt cggttgtgga
     3781 tgccgtcttg agggagatgg acctcgaggc ggatatcgct cccaccaagg acagagcttt
     3841 tatgccggga aggggggcgg acctcttcgc agaaggcaag aggatcggct gctttggaga
     3901 gctgcacccc ctgctcttgc gcagcttcgg cctggagcag cccgcggtag gtctggagat
     3961 cgagtgggga gagctggagc aataggaggc tctcctcatc ttcctcttcc tctccttttc
     4021 cagaagattt aaaacaagaa tataaatggt acgatattat taccttatca taatccgagc
     4081 gtctgattta tcttgataga gcggtaatag gtgaattgga aatacaaaac ggcggcttta
     4141 ttcttattgt ccgcccaaag gaatggtcgt gccatgaagg tcagagattt gatgagctat
     4201 cccattgtca ccgtgccccc tgaggccatg gtgctggatg ccataaaggt gatggccgcg
     4261 cagaagaagg gcagcgttct ggtggcaaaa gagcgcctcc tcaaggagtg tctgggaatc
     4321 gtcaccacca gccagatctt cctggaggtg tttgccaggg g
//
LOCUS       DAOSGP010000099         4297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_18514_length_4297_cov_15.169731, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000099 DAOSGP010000000
VERSION     DAOSGP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18514_length_4297_cov_15.169731"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLV49_03970"
     CDS             complement(<1..512)
                     /locus_tag="PLV49_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG98035.1"
                     /translation="MGWIEIWQRSRESWHWPLLPPPVLGEKDMSGSFAFSNYQITLDQ
                     QALQLGPLYLENLFHHLIVQYIFCPRSLEYAGILALAALKGLGHPSGSNSTYFSAYGS
                     MPGSASHIESARRMVNIFSDIVSDSFRLERSERDEELVLMGWRRLGELDGRPLNPLDR
                     VVLGFLRKFWG"
     gene            complement(499..1263)
                     /locus_tag="PLV49_03975"
     CDS             complement(499..1263)
                     /locus_tag="PLV49_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98036.1"
                     /translation="MKLGRFIDDAYRGLNKGRSLRSEKGDPIFSLDDLAERLGTRPSR
                     TEVEELLPQDPGTLERLVESDPGNRHQIIWGHLSSIAAERSHQPLHLPAGNYAGLELF
                     RGTIILEMGGDHVGERMKGGRIYLRQAAGDYLGQEMTGGGIVSADAGNYAFRRMSGGL
                     GVIKGDCGNFLGLGCSGGKVVCQGDCGERAGWLMSSGSLRIHGDAGDYLGLLMKGGRI
                     TVFGRAGRRAGWRMKGGAIRGKDFGPEAADGVLGLD"
     gene            complement(1260..2516)
                     /locus_tag="PLV49_03980"
     CDS             complement(1260..2516)
                     /locus_tag="PLV49_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG98037.1"
                     /translation="MPDPKEKIEDLIRALEELRLHVARNDLAVGNQRINSQLYFSIAN
                     ACLRNRALLLYGGMGANKTTLVNLLGSSFLSLHFGEVEDRMVTGYPEQTEEKMVGFLD
                     PRQWTREEDEPCTVLWTPWSRSRWKLINEVNRFPGGKQNLFLEILQKRKISYAGQVYE
                     PGDTCYYLTMNPEFSATYPLDEALLDRISVCVPAFQPNFLAGLALSERERGVWELAEE
                     LPRFTPQEFDSLPGLVAQKELEPRVELAVLCLVRDFTLCERAPSYDKTQLSSARPSRG
                     LCAGCHYSGNSEAICWQADGGLSDRVRQDLRSFSRALAYLLDREADLEVLKAVAPYVI
                     WHRINPIRSIIDRPPYYGARKLAFVADLVEKSINRTMNERADMNLIFSRAVDGETSVW
                     RAMEELSIFDDPIARLDFIPSLEQMK"
     gene            complement(3026..>4297)
                     /locus_tag="PLV49_03985"
     CDS             complement(3026..>4297)
                     /locus_tag="PLV49_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98038.1"
                     /translation="PLGLAYLASSLLAYDNDLDIEIIDGFILEREEYVKRISELRADI
                     IGVTATMPLLGEALKIPGMVKEKDALFIIGGPGVMNLPSTRLYEGGYSIVCYGEGERT
                     IVDLIRAQKKDLPLDKVRGISFISKGNEVRTPPREFIKNLDEIPFPSRHLLDMNKYTD
                     SWKKNMGVAFSPMVTSRGCQFSCRFCSKDIFGNKIRYRSPDSIIEEMRLLYDEYKIEM
                     IFFEDDLFTLNKKRVLDFCTVMERELPGKKWGAMSRVDTVDFETISRMKRAGCVELAF
                     GVESGSQAILDLLRKGITPEQIIRTFKQADEIGLVSGMFLIVGIPGETQEDMDMTKRL
                     IAESKPKWVNIAYLTPIPGTEIYEMTKHLIRDDVDFCDFNDSFGGVFKEGVFEVGPRE
                     RMKELMGFYLEEFKGRIDPRLSIYDGSMMAD"
BASE COUNT         1043 a         1245 c         1023 g          986 t
ORIGIN      
        1 ccccaaaact tccttaagaa gcccagaacc actcggtcta aaggattaag gggccggcca
       61 tccagttccc ccagcctcct ccagcccatc agcaccagct cttcgtctcg ctcgctcctc
      121 tccaggcgaa aggagtccga tacaatatct gagaatatat tcaccatcct gcgggcggac
      181 tcgatatgcg atgccgaacc gggcatcgaa ccataagcag aaaagtatgt ggaattagat
      241 cctgaaggat gacccagccc cttgagcgcg gccagagcga ggatacccgc atactccagt
      301 gatcgggggc agaagatgta ttgaacgatg agatgatgga agaggttctc caaatataga
      361 ggtccaagct gtagagcttg ctggtccaag gtgatctgat agttcgagaa ggcaaagctg
      421 ccagacatgt ctttttcgcc caggacggga gggggaagaa gaggccagtg ccagctttcc
      481 cgagacctct gccagatctc aatccagccc aaggactcca tccgccgcct cagggccaaa
      541 atcctttcct ctgatcgcgc cgcccttcat ccgccagccc gccctccttc ctgcccggcc
      601 aaaaacggtg atacgacctc ctttcatcaa gagccccaga tagtcgcccg catcaccatg
      661 aatgcgcagg cttcccgagc tcatcagcca gccagccctc tccccgcagt cgccctggca
      721 gaccaccttc cccccggagc agcccagtcc caggaagttg ccgcagtcac ctttgatcac
      781 tcccaaaccg ccgctcatcc tgcggaaggc atagttccca gcatctgcag atactatccc
      841 cccaccggtc atctcctgac ccagatagtc gcctgccgcc tggcgaagat atatcctccc
      901 ccctttcatc ctctcgccca catgatctcc tcccatctcc aggataatgg tgccccggaa
      961 taactccaga ccggcatagt tcccggccgg aaggtggagg ggctggtggc tcctctctgc
     1021 ggcgatgctg gagagatgac cccagatgat ctgatgtcta ttgcccggat cggattcgac
     1081 cagcctttcc agggttccag gatcctgggg cagaagctct tccacctccg tccggctggg
     1141 cctggtccct aacctctctg ccaggtcatc caggctgaag atggggtctc ctttctcgct
     1201 ccttagactg cgtcccttgt tcaggccgcg gtaggcatca tctatgaatc tgcccagctt
     1261 catttcatct gctccaggga gggtatgaag tccagccggg cgatgggatc gtcaaagatc
     1321 gatagctctt ccatcgccct ccagacggat gtctccccat ccactgctcg ggagaagatg
     1381 aggttcatat ccgcccgctc gttcatagtg cggttgatgc tcttttctac cagatctgcc
     1441 acaaaagcca gctttctcgc gccataatag ggaggccgat cgataatcga acggatggga
     1501 tttatccggt gccagataac ataaggagcc acagccttta aaacctccag atcggcctct
     1561 cgatccaaga gataggcaag agcccgggaa aaggatctaa ggtcctggcg cacccgatct
     1621 gacagccccc cgtccgcctg ccagcagatg gcctctgaat tgccgctgta atggcagcct
     1681 gcacacaggc ctcgcgatgg acgggcagag gatagctggg tcttgtcata tgacggcgcc
     1741 ctctcgcaga gggtgaagtc cctcaccagg cagagcactg ccagctccac ccgaggctcg
     1801 agctcctttt gagccaccag cccaggcagg gaatcgaact cctggggggt gaagcggggc
     1861 agctcctctg ccagctccca gacccccctc tccctttcag agagggccag ccctgccagg
     1921 aagttgggct gaaaagcggg cacacaaacg gatatgcggt ccagcagggc ctcgtccaga
     1981 ggataggtgg cggagaactc tgggttcatg gtcagatagt aacaggtatc tcctggctcg
     2041 tacacctgcc cagcatagct gatcttcctc ttctgcagta tctccaagaa caggttctgc
     2101 ttgccaccag gaaagcggtt gacctcattg atcagcttcc agcgggaccg agaccaaggg
     2161 gtccagagca ctgtgcaggg ctcgtcttcc tctctcgtcc actgtctggg atccagaaag
     2221 cccaccatct tctcctccgt ctgctccgga tagccggtca ccatccggtc ttccacctcg
     2281 ccgaagtgga gggataagaa tgaggacccg agcagattga ccagtgtggt cttgttcgca
     2341 cccattccgc cgtacaggag aagggcgcgg tttctcaggc aggcgttggc aatagagaag
     2401 tagagctgag agttgatccg ctgattgccg acggcaagat cattcctggc cacatgcagc
     2461 ctcagctcct ccaaagctct gatcagatct tcgatcttct cctttggatc tggcataaaa
     2521 agagggctat agtgcatacg atatttaaat taggcgagag gatggcggca gttggccaga
     2581 cgaagaggga aaacggctcc actcgaggaa agaagaggga cagcaaaagt cagtgtagga
     2641 gggattggtg attgactttt gccaccttag cttcgacgta agacgatata aaccttttgg
     2701 gtgtagacga tcagcgtgaa cttccgattc tgtgaaaagg agggaggggc aaaagtcacc
     2761 gtacgggagg gattgatggc cgtcgacttt tgcctgcaca ttaatacggc attcgacgat
     2821 ttaagctttt cggctgcacg ccgagatgct cattaaagag ataattagga ggggggcaaa
     2881 agtcattcaa ataggaggga ttgggaaata tgacctttgc acagacatag tacgatcatc
     2941 aacgtatata tttgtttccg tctgaagcaa tcgccgatga gataacacgc caggataaaa
     3001 acgaaatgaa ctgggaaacc gaattctaat ccgccatcat ggagccatca tatatgctca
     3061 atcttggatc aatcctgccc ttgaattcct ccaaataaaa gcccattaac tctttcatcc
     3121 tctcccgagg ccccacctca aacacaccct ctttaaagac tccaccaaat gaatcattaa
     3181 aatcgcagaa atcaacatca tccctgatca aatgctttgt catctcataa atctctgttc
     3241 cgggaatcgg agtgagataa gcgatattca cccatttcgg cttggactct gcaatcaatc
     3301 tcttggtcat atccatatcc tcctgggtct ctccaggaat tcccacaatc agaaacatgc
     3361 ctgagactaa tccaatttca tccgcctgtt tgaacgtcct gattatctgc tccggagtaa
     3421 ttcccttcct caagaggtct aagatggcct ggctcccact ctccacgcca aaggcaagct
     3481 cgacgcaacc tgctcgcttc attcgggata tcgtctcaaa atcaactgta tccaccctgg
     3541 acatggctcc ccatttcttc cctggaagct ccctttccat gacggtgcag aaatccagca
     3601 ctcgtttctt attcaatgtg aagagatcat cctcaaagaa gatcatttca attttgtact
     3661 catcataaag cagcctcatc tcctcgatta tgctatcagg agaccgatat cggatcttat
     3721 ttccgaatat gtctttgctg cagaatctgc aagagaactg acatcccctc gatgtgacca
     3781 tgggagaaaa ggcgactccc atattttttt tccatgaatc tgtatattta ttcatgtcca
     3841 gcaaatgcct tgagggaaag gggatctcgt cgaggttttt tataaattcc ctcggaggcg
     3901 ttcttacctc atttccttta gagataaaag agatgcccct taccttatcc agaggcagat
     3961 ctttcttttg cgcacggatc agatcaacga tggtcctttc accctcacca tagcatacaa
     4021 ttgagtagcc gccctcatag agcctggtgc ttggcaaatt catgaccccc ggtccaccga
     4081 tgataaagag ggcatctttt tctttaacca taccgggaat ctttagcgcc tcacccagca
     4141 gaggcatggt ggcggtgacc ccgatgatgt ccgcccttaa ttcagaaatt cttttaacat
     4201 actcttcgcg ctctaaaata aagccatcaa ttatttctat atctaaatcg ttatcataag
     4261 ccagaaggga tgatgccaga tatgccagcc ccagggg
//
LOCUS       DAOSGP010000100         4278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_18631_length_4278_cov_16.906228, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000100 DAOSGP010000000
VERSION     DAOSGP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18631_length_4278_cov_16.906228"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="PLV49_03990"
     CDS             complement(<1..1847)
                     /locus_tag="PLV49_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOG98039.1"
                     /translation="MTVGAAQPQPFQPADYSQGTSREWLVANGLGSYASSTANCSNTR
                     AYHGLLVAATEPPAGKRLLLASLDEELGEFRLANHQYPGAIYPQGFSHLQGFWTDPLP
                     RFCYQMGTTTLEKTVSMVRNENSTIISYRIKGYRGLIRIVPLIHDRSFHAAGELPPLH
                     QNTWGKGTTILSQTRFSIFSDLARYIPQERVYSNFEYEEERRRGLGWKENLFSPGCFE
                     LDVSGEAEFAIIASTWRSSLANWRADQESERERLISLKAPVPPLARAADSFLVKRGNA
                     LSLIAGYHWFDDWGRDAMIALPGLLLSTGRYEAARLVLSSFAGAMKDGVLPNDLGARS
                     YNTVDASLWFIQATSRYFDATGDLDFLAELWPKLLAVVERYSRPGDDFGADEDGLINS
                     GPALTWMDARVDGRPVTPRTGKCCEINALWYSALRRTESLARALDMPLNPKIPELADK
                     VQKSYIRFWNSENGCLFDVIDHEDASIRPNQIFAAIVPDLLPDVMRRSILEVVARDLL
                     TPFGLRTLSPRDPRYAARYAGGPRERDSSYHQGTVWPWLIGPYIDLLLAVGGRSAETR
                     EKGLDALRPLLDLAEVMGTIPEVFDGDLPQNPGGCISQAWSVGEVLRAY"
     gene            complement(1848..3326)
                     /locus_tag="PLV49_03995"
     CDS             complement(1848..3326)
                     /locus_tag="PLV49_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HOG98040.1"
                     /translation="MTLVSLSFEVHQPIRLKKNFFWDGYMNRQVVPDLWKYYFDDPEN
                     QRIFERVSDKCYLPANRAILEGIKKLQGSSRSFKVSYSFSGVFLEECQRYRPDVLDSF
                     VDLVQTGMVEVLDQTYYHSLASLYDDPQEYIEQIKMHRELIWDIFALRPTTFENTELI
                     YSDKIAQIADRMGYKAIFTEGVVADPNYVYRPPNTKIALLLRNYQLTDDIGFRFSSHN
                     WEEYPLTADKYASWLARTPGKCINIFCDYETFGEHQWIDTGIFDFIRSLPNQVLRYEN
                     LQFATPCEVAGTIPPERELSIESFVSWADLERNTSCWLGNALQHACFIQQKRLQAPAR
                     ESRDPDLLAIWRTLGLSDHLYYIFTHGGGPAEVHNYFSPYGSPYDAAVTYFAVLSDFH
                     CRLKGKTHLADSPFRFATGIDQFTGDSAWTLMGLEAILDKIPLSSLEYHNANGDYALW
                     AEKSLGDDALAEKLAGLQKLKGERLRKGLLRAVADSLATEVI"
     gene            3464..>4278
                     /locus_tag="PLV49_04000"
     CDS             3464..>4278
                     /locus_tag="PLV49_04000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOG98041.1"
                     /translation="MKALNAERYYLDLLIPVFLILLAEILIFYGKPEAAMPIHALNLV
                     FLSLSTIFVNNRTYPALMLLPLFRLLNVAMPVFFHLTLYSYVLVYAPMFIPMYMILKE
                     RFITAEEAGLTFERFWFFLPVSISMGLALGWGEYMVIKPQMLVVDNDLQQMLILALTM
                     IFFVGIVEEFVFRSSLQTVLQERIGLIGGLVLASVIFGFMHSGYHLTLELLYVSLAGL
                     AFGVLFWLSKSLPIVSLAHGVTNIALFLVAPLKPEILPYLIIGPLFLFFFYAYA"
BASE COUNT          817 a         1156 c         1300 g         1005 t
ORIGIN      
        1 tcataagccc tgagaacctc tcccaccgac caggcctggg agatgcagcc accgggattc
       61 tggggcaggt cgccatcgaa gacctcggga atggtgccca tcacttctgc cagatccaag
      121 agcggcctta gtgcatccaa tcccttctcc cgcgtttcgg cggagcggcc gcctaccgcc
      181 agcaggagat cgatataggg gccgatgagc cagggccaga ctgtgccctg atgatagctg
      241 ctgtccctct ccctgggtcc tccagcatat cgggccgcat aacggggatc gcggggggag
      301 agggttctca ggccgaaggg ggtgagaaga tctcgagcta ccacctccag gatgctcctt
      361 ctcatcacat ccggcaggag atcgggaacg atcgccgcga agatctggtt ggggcggatg
      421 gaggcgtcct catggtctat cacatcgaag aggcagccgt tctcgctgtt ccagaatcgg
      481 atgtagcttt tctggacctt atccgcgagc tcgggaatct tcgggttcag gggcatgtcc
      541 agggcccggg caaggctctc tgtcctcctc agggcggagt accagagggc gtttatctcg
      601 cagcatttgc ccgtccgggg ggtgaccggc cggccgtcca ctctggcgtc catccaggtg
      661 agagccgggc cggaattgat caggccgtcc tcatctgcgc cgaagtcatc gcccggtcgg
      721 gagtatctct cgaccacagc caagagcttc ggccagagct cggccaggaa atccagatca
      781 cctgttgcat cgaaatatct gctcgtcgcc tgaataaacc acaaagaggc gtccacggtg
      841 ttgtaggacc gggccccgag gtcgttgggc agcacaccgt ccttcatcgc cccggcgaag
      901 gagctgagga ccagccttgc tgcctcatat cggcctgtgg agaggagaag gccgggcagg
      961 gcgatcatgg cgtcccttcc ccagtcatcg aaccagtggt agccggcgat gaggctcaga
     1021 gcgttgcccc tcttgaccag gaagctgtcc gccgctcgag ccagaggagg aacgggtgcc
     1081 tttaaggata tcaggcgttc tctctcgctc tcctgatccg ccctccagtt ggccaaggag
     1141 ctcctccagg tggaggcgat gatggcgaac tccgcctctc cggatacatc cagctcgaag
     1201 cagccggggg agaagaggtt ctccttccag cccaggcccc ggcggcgctc ctcctcgtac
     1261 tcgaagttgc tgtagaccct ctcctgggga atatatcggg ccaggtcgga gaagatggag
     1321 aaacgggttt ggctgaggat tgtggttcct ttgccccagg tattctggtg aagtggagga
     1381 agctcgccgg cagcatggaa gctgcggtcg tggatcaggg gtacgatgcg gatcaatcca
     1441 cggtagccct tgatcctgta gctgatgatg gtgctgttct cattgcgtac catggagacg
     1501 gtcttctcca gggttgtagt gcccatctga tagcagaacc gggggagggg atcggtccag
     1561 aatccctgca ggtgagagaa gccctgggga tagatcgctc cggggtactg atgattggcc
     1621 agtcggaatt cgcccagctc ctcgtccagg ctggcgagga gcagtctctt ccctgccgga
     1681 ggctcggtgg ctgccaccag aaggccgtga taggctctgg tgttgctgca gtttgcggtc
     1741 gatgaggcat agctgcccag gccgttggcc accagccact cccgggatgt cccctgggaa
     1801 tagtcggccg gctggaaggg ttgaggttga gctgctccta cagtcattta gatcacctct
     1861 gtggccaggg aatcggccac tgcccggaga aggccctttc tcagcctttc ccctttgagt
     1921 ttctgcaatc cggccagctt ttccgccagg gcgtcatcgc ccaggctctt ctccgcccag
     1981 agggcataat cgccgttggc attatggtac tccaaagagg agagagggat tttgtccagg
     2041 atggcctcca ggcccatcag tgtccaggcg gagtcgcctg tgaactggtc tattccggtg
     2101 gcaaaccgga atggggaatc ggccagatgg gtctttccct tcaggcggca gtgaaagtcg
     2161 gataagacgg caaagtaggt tacagcggca tcataggggg agccgtaggg gctgaagtag
     2221 ttgtgcacct cggccggccc tccgccgtgg gtgaatatgt agtagagatg gtcggagagg
     2281 cccagagtcc tccagatggc caggaggtcg gggtcgcggc tctctcttgc cggtgcctgg
     2341 agcctcttct gctggatgaa gcaggcatgc tgaagggcgt ttcccagcca gcagctggtg
     2401 tttctctcca ggtctgccca ggagacgaag ctctctatcg atagctctct ttccggcgga
     2461 attgtgccgg ccacctcgca gggggtggcg aactgaaggt tctcgtagcg gaggacctgg
     2521 tttggcaggc tgcggatgaa gtcgaagatc ccggtgtcga tccactggtg ctcgccgaag
     2581 gtctcgtagt cgcagaagat gtttatgcac ttgcctgggg tcctcgccag ccaggaggcg
     2641 tacttgtctg cggtgagggg gtactcttcc caattatggg aggagaagcg aaagccgatg
     2701 tcgtcggtga gctggtagtt acgcaagagc agagcgatct tggtgtttgg cgggcggtag
     2761 acgtagttgg gatctgctac caccccttcg gtgaatatgg ccttatagcc catccggtcg
     2821 gcgatttggg cgattttgtc gctgtagatc agctcggtgt tctcaaaggt cgtaggccgg
     2881 agggcgaaga tgtcccagat cagctcccgg tgcatcttga tctgctcgat gtactcctgg
     2941 ggatcgtcgt agaggctggc caggctgtga tagtaggtct gatccagcac ctccaccatt
     3001 ccagtctgga ccaggtcgac aaaggagtct aacacatccg ggcggtacct ttggcattcc
     3061 tccaggaaga cgcctgagaa gctgtaggag accttgaagg atctggacga tccctggagc
     3121 ttttttatcc cctccaggat ggcccggttg gcgggcaggt agcatttgtc ggatacccgc
     3181 tcgaagatcc tctgattctc tgggtcatcg aagtagtatt tccataaatc aggtaccacc
     3241 tgccggttca tgtagccgtc ccagaagaaa ttcttcttca gacggattgg ctggtggacc
     3301 tcaaaagaga gggagacaag ggtcatacta tatcatcagg gctgcaaatt atttacatcc
     3361 ttcgcattat atctgcccaa gtcagggcta aagtatgctg attttttggt acagtaaggt
     3421 tcgagggaat ctattaaatc tttgatgaga acatggcaat tatatgaagg cgctgaatgc
     3481 ggagaggtac tatctcgacc tgctgattcc cgtatttttg atactgttgg ccgagattct
     3541 gatcttctac ggcaagcctg aagcagcgat gcccattcat gcgctgaacc tggttttttt
     3601 aagcctatca acgatattcg tcaacaaccg gacctatcca gccctaatgc tcttgccact
     3661 gttccgcctt ttgaatgtcg cgatgcccgt cttcttccac ctgaccctct actcctatgt
     3721 gctggtttat gcgcccatgt tcattcccat gtatatgatc ttgaaagaga gattcataac
     3781 cgcagaggag gcgggattga cctttgagcg gttctggttc tttctgcccg tttccatatc
     3841 catgggactg gctttggggt ggggggagta tatggtgatc aagcctcaaa tgctggtggt
     3901 ggacaatgat ctccagcaaa tgctcatttt agccctgacc atgatcttct ttgtgggtat
     3961 tgtggaggag ttcgtatttc ggtcgtcttt gcagactgtg ctccaggaga ggatcggtct
     4021 gattgggggg ctggtcttgg ccagtgtgat attcgggttc atgcatagcg gctatcacct
     4081 gacactggag ctcctctacg ttagcctggc ggggctggct ttcggggtgc tcttctggct
     4141 ctccaagagc ctgcctattg tctccctggc ccacggggtc accaatatag cgctgttcct
     4201 cgtcgctccc ctcaaaccgg agatcctgcc gtatttgatc ataggcccgc tattcctgtt
     4261 cttcttttac gcttatgc
//
LOCUS       DAOSGP010000101         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_18827_length_4245_cov_15.547255, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000101 DAOSGP010000000
VERSION     DAOSGP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18827_length_4245_cov_15.547255"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLV49_04005"
     CDS             <1..674
                     /locus_tag="PLV49_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG98042.1"
                     /translation="EDVVVTGDIGCYSLGAFQPLDRMDSILCMGAGVSMAHGMEKAGE
                     KRKVVGVVGDSTFFHSGITGLLDIVYNKGHSTIIVLDNRTTAMTGHQDHPGTGRTISG
                     EPTVAVSIEDIGRACGIKRVRTIDPYQLDDAREAIKEELAALEPSLIVSRAPCPLRER
                     RRVGAMRTILAEECRACELCLSLGCPAIEGVDGVMKINQTLCAGCGLCEQICPAGAIV
                     SREGA"
     gene            671..1342
                     /locus_tag="PLV49_04010"
     CDS             671..1342
                     /locus_tag="PLV49_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOG98043.1"
                     /translation="MIERLVEGTEGMKNQESLKSKNSATNSPDRSPKETKSIVLVGVG
                     GQGILLASEILAQAAMIQGYQVKTNEVHGMAQRGGSVMAQIRYGEEVKSPLVSKRSAH
                     ILGALEKVEALRYWEFLRPDGLAAVSSQAIVPVTVSSGLARYPENVEELLARAFPRLV
                     YIDAPEMAKGLGDSRAANIILLGAISRGLDLPLESWEEAIGRCVRERFRDLNLKAFSL
                     GRSKA"
     gene            1339..>2021
                     /locus_tag="PLV49_04015"
     CDS             1339..>2021
                     /locus_tag="PLV49_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HOG98044.1"
                     /translation="MTQKGNFHNESMETASRESIHELQSRRLRAIVGHVYESNAIYRR
                     LFDEKGIRPEDIRTAEDVVRLPFTGKDILRESYPLKMACVSRDQIVEMHMSSGSTGTP
                     VVMPYTRSDLDQWAECMARCYRMSGAKPGDATQITPLFGLFNGGFGMYHGARAAGLFV
                     IPAGPGNTARQIRLARDLKTRVFTGVVSYGIRLMEVLAEEKESLPELEIGIFGAEVFS
                     DSMKKKISSG"
     assembly_gap    2022..2121
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2122..2777
                     /locus_tag="PLV49_04020"
     CDS             <2122..2777
                     /locus_tag="PLV49_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HOG98045.1"
                     /translation="IFGAEVFSDSMKKKISSGLDIDVFDIYGMTESGGVGTLGMDCPA
                     HDGIHVWEDQYILEVIDPKTGEPVDDGEEGELVFTSLTRQALPMIRFRTADLTHVISR
                     DRCECGRTHVRLAPITGRRDDMLIVKGVNFFPKQIEQTLLGIPGIGNNYQIIVEEIDG
                     VNSVRVNVEADPGVTGYMVEKALKEALGFSPKGDVFPLGGLPRQEGKAKRVFHNRIK"
     gene            2872..4038
                     /locus_tag="PLV49_04025"
     CDS             2872..4038
                     /locus_tag="PLV49_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98046.1"
                     /translation="MRFNCLCLCLLALVMGLGVAAGYDVSISQSMEVSETGFDEEFVK
                     SLFGFGAEGGEGFWFGPEFEDVSTATTFFSEFYMESSVPVVKTFAPVKIDVTQNVPSR
                     IYFGSGKEIAYTQYQSTVATSRGNELWIQKGADWSQYAIVPAGSGIQFIAFAPVGGQA
                     DYYWMLQSNSLNITSKRLDLYPGYNSLNFKADEVGRHILLFVLNNQPSNAIIIDVISQ
                     APPAQQTAVAADMPPSTNNPAASGVQAMQTVQPTQAVQMLPGATTSVMQTYSGTAQQT
                     TSASSTTQYPAAVATKPAVPAIVAGDTPVTIQTTLKGYDVYVDGVLIGKEGTGGDLLD
                     GVFKFKVVGGMIHNIRVFDGEHNYPKDIYFERGVQKIINVPPGTTVYGIGTPYY"
BASE COUNT          982 a         1086 c         1227 g          850 t
ORIGIN      
        1 gggaggatgt ggtggtcacc ggggacatcg gatgctacag cctgggcgct tttcagcccc
       61 tggaccggat ggacagcatc ctctgcatgg gagcaggagt ctccatggcc cacggcatgg
      121 agaaagcagg ggagaagaga aaggtggtgg gcgtggttgg agactccacc ttctttcact
      181 ccgggatcac tggtctcttg gatatcgtct acaataaggg ccactccacc ataatcgtac
      241 tggacaaccg caccactgcc atgaccggcc accaggacca tccgggcacc ggcaggacca
      301 ttagcggcga gcccacagtc gccgtctcca tcgaggatat cggccgggcc tgcgggatca
      361 agcgggtccg gacgatagat ccctaccagc tggatgacgc cagagaggcg atcaaagagg
      421 agctggcggc tcttgagccc tctcttattg tatcccgcgc tccctgcccc ctgcgggaga
      481 ggagacgggt gggggcgatg agaaccatcc tggcggagga gtgccgggcc tgcgagctgt
      541 gcctctcttt gggctgcccg gcgatagagg gagtggatgg ggtgatgaag atcaatcaga
      601 ccctctgtgc gggctgcggc ctgtgcgaac agatctgccc tgccggggcg attgtcagcc
      661 gggagggagc atgatcgaga ggttagtgga agggacagag ggaatgaaaa accaggagag
      721 cttaaagagc aaaaacagcg caacaaactc accagacagg agcccgaaag agaccaagag
      781 catcgtcctc gtcggcgtgg gaggtcaagg gatactgctg gccagcgaga tattagccca
      841 ggcagcgatg attcagggct accaggtcaa gaccaatgag gtgcacggaa tggcccagcg
      901 ggggggctcg gtgatggccc agattcgtta tggagaagag gtcaagagcc ccctggtctc
      961 caaaaggagc gctcatatcc tgggtgcgct ggagaaggtg gaagccctgc ggtactggga
     1021 gtttctccgg ccggacggcc tggccgcggt cagcagccag gccatagtgc cggtgaccgt
     1081 ctcctccggt cttgctcgct acccggagaa tgtagaggag ctgctggcca gggcctttcc
     1141 ccggctggtc tatatcgatg ccccggaaat ggcgaagggg ctgggtgata gcagggcggc
     1201 caacatcata ctgctgggag caatttcccg gggactggac ctccccctgg aatcctggga
     1261 ggaggccatc ggccgatgcg tccgggagag gttccgagat ctcaatttga aagcgttttc
     1321 acttggcagg agcaaagcat gacacagaag ggcaattttc acaatgaatc gatggagacg
     1381 gcatccaggg agagcattca tgagctgcaa tcccggcggc tgagggccat cgtcggtcac
     1441 gtctatgaat ccaatgccat ctacaggcgt ctattcgatg agaagggcat ccggcctgag
     1501 gacatcagaa cagcagagga tgtggtccgg ctgccattca ccggtaagga tatcctgaga
     1561 gagagctatc ccctgaagat ggcctgcgtt tcccgcgatc agatcgtgga gatgcatatg
     1621 tccagcggct ccaccggcac cccggtggtt atgccctata cccgctcaga cctcgatcag
     1681 tgggcggaat gcatggcccg ctgctatcgc atgtccgggg cgaagcctgg ggacgccacc
     1741 cagatcacac cccttttcgg ccttttcaat ggcggctttg gcatgtatca tggcgcccgg
     1801 gcagcaggcc tgttcgtgat ccccgccggt ccgggaaaca ccgcccggca aatccgccta
     1861 gctcgagatc tgaagacgag ggttttcacc ggagtggtga gctatggcat ccggctgatg
     1921 gaggtgctgg ccgaggagaa ggagagcctg ccggagctgg agataggcat cttcggagca
     1981 gaggttttct ctgactccat gaaaaagaag atctcctcgg gnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn ngcatcttcg gagcagaggt tttctctgac tccatgaaaa
     2161 agaagatctc ctcgggcctg gacatcgacg tcttcgacat atacggcatg actgagtccg
     2221 gaggtgttgg cactttaggc atggactgcc cggcacacga tggaatccac gtctgggagg
     2281 atcagtacat cctggaggtc atcgatccaa aaacgggaga gccagtggat gacggggagg
     2341 aaggggagct ggtcttcacc tcgctcaccc ggcaggctct gcccatgatc cgtttccgga
     2401 cagctgacct cacccatgtc atatcccggg atagatgcga atgcggcaga actcacgtcc
     2461 gtctggcacc gataaccggg cgaagggatg acatgctgat tgtgaaaggg gtgaacttct
     2521 tccccaagca gattgagcaa accctcctgg gaataccggg catcggcaac aactatcaga
     2581 tcatcgtgga agagatagac ggggtcaata gcgtccgggt caacgttgaa gcagatccgg
     2641 gtgtgaccgg ctatatggtg gaaaaggcgc tcaaagaggc gctgggattc tcccccaaag
     2701 gagacgtctt ccccctggga ggcctgcccc gccaggaagg aaaggcaaag agggtgtttc
     2761 ataacaggat aaaataactc tttttctctt ccatgatatt cagatgagcc tgatacaaga
     2821 cttaaggcta aatagataca agtccagtca gtattcattg aggtgcaata gttgagattc
     2881 aattgcctat gcttatgtct cttggccctg gtcatggggt tgggagtggc agctggatat
     2941 gatgtatcca tatcgcagtc catggaggtc tccgagactg gcttcgatga agaattcgtc
     3001 aagagcctgt ttggattcgg cgccgaaggt ggggagggtt tctggtttgg cccggagttc
     3061 gaggatgtca gcaccgccac caccttcttt tccgagttct atatggagag ctctgtccca
     3121 gtggtcaaga ccttcgctcc ggtaaagatc gatgtgactc aaaatgttcc ttctcggata
     3181 tatttcggca gcggcaagga gatcgcatat acccagtacc aatctacagt ggccaccagc
     3241 agaggaaatg agctctggat tcagaaagga gctgactggt cccagtatgc cattgttcct
     3301 gcgggatccg gcattcagtt catcgccttt gcccccgtgg gcggccaggc ggactactat
     3361 tggatgcttc agagcaactc cctcaatatc accagcaagc gtctggatct ctaccccgga
     3421 tacaacagcc tgaacttcaa ggctgatgag gtgggcaggc acattctcct ctttgtgctc
     3481 aataatcagc ccagcaacgc catcatcatc gatgtgatca gccaggcccc tccagcccag
     3541 cagacggcag tggcagctga tatgccacca tccaccaaca accctgcagc ctctggagtg
     3601 caggccatgc aaaccgtaca gcctacgcag gcggtgcaga tgctccccgg agccacaacc
     3661 agcgtcatgc agacatacag cggaacggct cagcagacaa cctctgccag cagcactact
     3721 caatacccgg ctgctgtagc gacaaagcct gccgtgcctg caattgttgc cggagacaca
     3781 cccgtgacca ttcagaccac cttgaagggc tatgatgtct atgtggacgg agtactcatt
     3841 ggcaaagagg gaaccggtgg cgaccttctg gatggtgtct tcaagttcaa ggtggtgggc
     3901 ggaatgatcc ataacatcag ggtcttcgat ggagagcaca actacccgaa ggatatttac
     3961 ttcgagagag gagtgcaaaa gatcataaat gtgccaccag gcactactgt ctatggaata
     4021 ggcacaccct attattgaca gatgcctgat catttataac ctatttttta aaaccctcat
     4081 gattctgcga ctcatacacg caagatgaaa atcgatatac ttagagacat tttcatggga
     4141 aaaattaagg agtgagcatc ccaattctcc atctggggag aggttatcag taaatgttat
     4201 tttattcata attaatgata ttttatgata ccagaatttg gctgc
//
LOCUS       DAOSGP010000102         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_18852_length_4242_cov_17.848340, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000102 DAOSGP010000000
VERSION     DAOSGP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18852_length_4242_cov_17.848340"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLV49_04030"
     CDS             complement(<1..544)
                     /locus_tag="PLV49_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98047.1"
                     /translation="MLDEFARYTALQRDQMPLAMLLVYLKRKGGFVQLNQIWRELGPT
                     GGGPFWNQTTLINKLDKLERLGVVVMEKRILPSPRAEAKKKTNTFYRLSPATPLYPYV
                     YGMLKIYKEEQDRYSSQLVDKPLDHLCRLAGRSSASDPLIGRELEVAKELISEVFSVG
                     GDEVRMMIKERMVRKGMIRDR"
     gene            678..>1288
                     /locus_tag="PLV49_04035"
     CDS             678..>1288
                     /locus_tag="PLV49_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG98048.1"
                     /translation="MRPLTAGVPKVMLPIAGRPLLEHIVLRAKEAGIERFVLVVGYGA
                     DSVRDHFQDGRSLGVKIEYAHQNEQLGTAHALMAAESLAEGSFMVLNGDVLPDVGALK
                     ELAAKGMAVSAIKVDDPSRYGVFLEEDGIFQSVVEKSEDPPSNLANAGIYLFKKWIFD
                     ELRVIPKSSRGEYELTDGLNRAAKKEAIEIVELNSWLEIGRPW"
     assembly_gap    1289..1388
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1389..1570
                     /locus_tag="PLV49_04040"
     CDS             <1389..1570
                     /locus_tag="PLV49_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOG98049.1"
                     /translation="KGEKVDSGRRKLGVIMGHGVMTGINTSIYPGTVIEAGYWGRPAE
                     VIRGHISPEMKDNGL"
     gene            1641..2741
                     /locus_tag="PLV49_04045"
     CDS             1641..2741
                     /locus_tag="PLV49_04045"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG98050.1"
                     /translation="MRDFISIAKPTVGEEEIEAVGEVLRSGMLTQGEKVKNFEEEFSE
                     YLGVGHSIAVSNGTVALDLALKGLGLEPGDEVISPAFTFIATANTVLYQGSVPVFADV
                     DPRTFNIDPDDLSEKITPRTRAVIGVHLYGQPFDAKAVCQICEDHNIALVEDCAQAHG
                     AMIGGRMAGSFGTGCFSFYPTKNMTTGEGGMITTNDDALADRLRLLRNHGDTGKYNHI
                     SIGYNYRMTNIQGAIGSGQLKRLEGFIAKRIENADTLNKTIKIDGLTVPFRMADVRHV
                     YNQYVLRVEDDFPVSREKLMEYLNANGIATAVHYPKAVYEQPIYQKLGYGQDRCPVSE
                     DVSRRVMSLPVHPSLSASDLQHIAQTINCFED"
     gene            2744..3670
                     /locus_tag="PLV49_04050"
     CDS             2744..3670
                     /locus_tag="PLV49_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG98051.1"
                     /translation="MDVGVIGVGAMGKNHVRVYSELKGVDTVYVYDPISENAQRASDL
                     ATVCSSPEELLARAEAVSVCVPTRYHFDTASKVINAGVDCLIEKPITMTVQEAERLLG
                     LIEKTDLVVGVGHIERFNPIVEEIKKIAERPDYVSIKRHNPTSNRITDASVVEDLMIH
                     DIDIVFNVLFKGEEDYQIFSAGSRNVCEAMAVFSESVVSISASRLSSKKFRTFYVEAE
                     EFTTEGDFMTQEVYVYRKPGKYRVEDERYLQENIIEKVLVNKVEPLKVELKTFLDCAR
                     QNKSFPVTPGEGKKNLEICERIKSGLNARKGP"
     gene            3707..>4242
                     /locus_tag="PLV49_04055"
     CDS             3707..>4242
                     /locus_tag="PLV49_04055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG98052.1"
                     /translation="MTKSQGNSKGNSQDESQAYYAHPTAIVESESIGEGTKIWHFAHV
                     RRSSKIGKGCNIGKSVYIDIGAEVGDNVKIQNFVSVYKGVKIEDDVFVGPSATFTNDL
                     YPRAFIWDEEHVLATRICRGSSIGANATIVCGITIGEYAMIGAGSVVAEDVPSYALIL
                     GNPGRQRGWVCRCGRRLNT"
BASE COUNT          994 a         1079 c         1219 g          850 t
ORIGIN      
        1 ttcgatcccg tatcatcccc ttgcggacca tcctctcctt gatcatcatc cgcacctcat
       61 cgccgcccac gctgaagacc tcggatatca attccttcgc cacctccagc tccctgccga
      121 tgaggggatc ggacgcactc gaccttcccg ccaggcgaca gagatggtct aagggcttgt
      181 caaccagctg ggaggagtat cggtcctgct cttctttgta gatcttgagc atgccataga
      241 cgtagggata aagaggtgtg gccggactga gccggtaaaa ggtgttggtc ttcttcttag
      301 cctcggcacg gggagagggg aggattctct tctccatgac cactacccct aacctctcca
      361 gcttgtccag cttatttatg agggtggtct ggttccagaa tggtcccccg ccggtgggcc
      421 ccagctcccg ccagatctgg ttcagctgca caaacccccc cttcctctta agatagacga
      481 gcagcattgc cagaggcatt tgatcgcgct gcagggcagt atagcgggcg aactcatcca
      541 acatgaggac ctctgggaca aataaccgta aagccttttc ggtttttttg cagaagcatt
      601 tttatccccc ttgcatcctc agatctcagg gttagacaat gcaagcgatc attctcgccg
      661 cgggagaagg aagcaggatg cgcccactga ccgcaggggt gccaaaggtc atgctcccaa
      721 tagctggcag gccacttctc gagcatatcg ttctccgggc caaagaggca ggaattgaga
      781 ggttcgtgct ggtggtgggt tacggcgccg attcggtgcg ggatcatttc caggacggcc
      841 ggagccttgg ggtgaagata gaatatgctc accagaatga gcagctggga actgcccatg
      901 cgctgatggc ggcggagagc ctggccgaag gcagtttcat ggtattgaac ggcgacgttc
      961 ttcccgatgt tggcgcactg aaggagctgg ctgccaaggg tatggcggtc tcggcgatca
     1021 aggtcgatga cccaagcagg tacggcgtct ttttggagga ggacggcatc ttccagtcgg
     1081 tggtggagaa gagcgaagat cctccctcaa acctggccaa tgcaggcatc tatctcttca
     1141 agaaatggat cttcgatgag ctgcgggtaa tacccaaatc ctctcggggc gagtacgagc
     1201 tgacagacgg cctgaaccgg gcggcaaaaa aagaggccat agagatcgta gagctgaaca
     1261 gctggctgga gatcggccgg ccctgggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnntc aaaggggaga aggtagacag cggcaggcgc aagcttggtg tgatcatggg
     1441 ccatggagtc atgaccggga tcaacacctc aatctatccg ggaacggtga ttgaggccgg
     1501 ctactggggc aggcctgcag aggtcatacg gggccatata tctccagaaa tgaaagataa
     1561 tgggctttag tgacaagcct tttcaagggt gaagatgagc acaggatata gcgaccagaa
     1621 caggatacaa ggagatcttg atgagagatt ttatttccat agccaagccc acggtggggg
     1681 aagaagagat agaggcggtg ggagaggtgc tgaggagcgg catgctcact cagggcgaaa
     1741 aggtcaagaa ttttgaagag gagttcagcg agtatttggg tgtagggcat tctattgccg
     1801 tcagcaacgg cactgtggcc ctggacctgg ccctgaaagg attggggctc gagccgggag
     1861 acgaggtcat ttcaccggca ttcaccttta ttgctaccgc caataccgtt ctctaccagg
     1921 gctcggtgcc cgtcttcgct gatgtggatc ccaggacgtt caatatcgat ccagacgacc
     1981 tctcagagaa gatcacccct cgtacccgag cggtgatcgg ggttcacctc tacggccagc
     2041 ccttcgatgc caaagccgta tgtcagatct gcgaggatca taacatcgcc ctggtggagg
     2101 attgcgctca ggcccacggt gcgatgatcg ggggaaggat ggcgggctca ttcggaaccg
     2161 gctgtttctc cttttatcct acaaagaaca tgaccaccgg tgagggaggc atgatcacca
     2221 ccaacgatga cgccctggcc gatcgcctgc ggctgctccg aaaccacggt gacaccggaa
     2281 agtacaacca catatcaata ggctacaact accggatgac gaacatccag ggtgccatag
     2341 gctcgggcca gctcaagcgg ctggagggat tcatcgccaa gaggatcgag aatgccgata
     2401 ccttgaataa aacgatcaag atcgatggac tgactgtgcc cttccggatg gcggatgtac
     2461 gccatgtcta caatcagtat gtcctgcggg tggaggatga cttccccgtc tccagagaga
     2521 agctgatgga gtacctcaat gccaatggca tcgccaccgc agtacattat cccaaagcgg
     2581 tctatgagca gcccatctac caaaagctgg gttatggcca ggataggtgc ccggtgtcag
     2641 aggatgtatc ccgccgggtg atgagccttc ctgtgcatcc ctcattgagc gcctctgatc
     2701 tccagcatat cgcccagacc ataaattgct tcgaggactg aacatggacg ttggagttat
     2761 tggcgtcggg gccatgggaa agaaccatgt gcgggtctac tcggagctga aaggggtaga
     2821 tacagtctac gtctacgatc ccatatcgga aaatgctcag cgtgccagtg acctcgccac
     2881 tgtctgctca agccccgagg agctgctggc acgggcagag gcggtaagcg tatgcgttcc
     2941 cactcgatac cacttcgata ctgccagtaa ggtcataaac gcaggggtag actgcctgat
     3001 cgagaagccc atcacaatga ctgtccagga ggcagagcgt ctcttggggc tgatcgagaa
     3061 gaccgatctt gtggtggggg tgggccatat cgagaggttc aatccaatcg tggaggagat
     3121 caagaagatc gccgagcgac cggactacgt ctccatcaag agacataatc ccacctccaa
     3181 ccgcataacc gatgcctcag tggtcgagga cctcatgatc catgatatcg acatagtctt
     3241 caatgtcctc ttcaaagggg aggaggacta ccagatcttc agcgccggca gccgcaacgt
     3301 ctgcgaggcg atggcggtct tctccgaatc cgtggtctcc atctccgcca gccgcctgtc
     3361 ctccaagaag tttcgcacct tctatgtgga ggcagaggag ttcaccaccg agggcgactt
     3421 catgacccag gaggtatacg tctaccgaaa accaggaaaa taccgggtag aggacgagag
     3481 gtatctgcag gagaacatca tcgagaaggt tctggttaac aaggtagagc ccctcaaggt
     3541 ggagctgaag acgttcctgg actgcgcgag gcagaataag agcttcccgg tcactccagg
     3601 agagggcaaa aagaacctgg agatctgcga gagaatcaaa tcgggattga atgccaggaa
     3661 ggggccataa gatgatgacc atctcttctg cctcatggag accgaaatga caaaatccca
     3721 gggcaattct aaaggcaatt ctcaggatga gtcccaggcc tattatgctc atcccactgc
     3781 tattgtggag agcgaatcca tcggcgaggg aacgaagatc tggcactttg ctcatgtgcg
     3841 ccggagctct aagatcggta agggctgcaa tatcggtaag agcgtctaca tagatatcgg
     3901 ggcagaggta ggggacaatg tgaagatcca gaacttcgtc tccgtctaca agggagttaa
     3961 gatcgaggac gatgtcttcg tgggcccatc agctacattc accaatgacc tctatcctcg
     4021 cgcctttatc tgggacgaag agcacgtctt ggcaaccagg atctgccggg ggtcgagcat
     4081 tggggccaat gccaccattg tatgcggaat aacaataggc gagtatgcca tgatcggagc
     4141 gggaagcgtg gtggcagagg acgtgccatc ctatgccctg atcctgggca atccgggccg
     4201 gcagaggggt tgggtctgcc gctgcggccg tcgtctgaat ac
//
LOCUS       DAOSGP010000103         4220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_18992_length_4220_cov_19.730852, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000103 DAOSGP010000000
VERSION     DAOSGP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18992_length_4220_cov_19.730852"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLV49_04060"
     CDS             <1..794
                     /locus_tag="PLV49_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98053.1"
                     /translation="ATEALVTPALMVMMAFSLTEVDLGRDIKSSGSLKSGLLGIIINY
                     GLLSGLILLLSYSLPDESLRNGFVIMAAVPPAVAVLPMTRILKGDMSLSFAGEILSYL
                     ASLLLMPLIIYLFVHQTGISPIYLLQISIVLILIPALASRLVRLIPINPVLPINLGFF
                     LVTYTVIGLNQGALWTDGWSVAWISIARTFAVGLAVFILARSAGLKSSQAISLTLLGS
                     FKNLGLAAAVSLLLVGPAAGLPAAFCVLAETAFFILLSFARSRYP"
     gene            830..1414
                     /locus_tag="PLV49_04065"
     CDS             830..1414
                     /locus_tag="PLV49_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOG98054.1"
                     /translation="MSIKDDAAYLRSIYIYVALSVFLFALTAVMGYYTAQFDPEFAAS
                     WTEELEMLAWILEQPPLMIMIIIFLKNLLASATAMLLGLGLGIVPMIVATTNGFLLGI
                     VGYSAVEQKGWLYLAAGILPHGIIELPAVLLSIAIGLRLGHMLALSLLKESSDLSGEM
                     RVAIHFLLRWIMPLLLLAAAIETFITPFIISRVA"
     gene            1411..2874
                     /gene="proS"
                     /locus_tag="PLV49_04070"
     CDS             1411..2874
                     /gene="proS"
                     /locus_tag="PLV49_04070"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOG98055.1"
                     /translation="MTYPDTKNKGKGESGGQSAKLSPKEDFSQWYHELLMTGEIIDNR
                     YPVKGMCVWFPFGFAIKKNVYGIIRQLLDPDHQETLFPLMIPENEFMKEAQHIKGFEE
                     EVYWVTCGGTTPLEVKLALRPTSETAIYPMFKLWIRSHADMPLRIYQIVNTFRYETKH
                     TRPLIRLREITSFKEAHTVHATWEEAAEQVEVAIKRYSEFYRRLAIPFLVSRRPSWDK
                     FPGADYTTAIDVIMPDGKTLQVGTAHHLGSTFAKTYEITYEAEDGEQKLVNQTCYGIS
                     ERCIAALISVHGDDKGLALPWEVAPVQVVIVPILLGDKEKVLEVCRDLLRILQQAGIR
                     VELDTSDERPGAKFYKWEMKGVPIRLEVGPRDIEKGVVTVVRRDAEKLTLPREGLADA
                     LIGEAEKLQAALYQRAEAFANSRIREVASMDEARAQTEAGVALVPWCGEEECGHQLED
                     QVGANMLGEPQYKSFSPAACAVCGKLTDKRTYMARQY"
     gene            2902..3564
                     /locus_tag="PLV49_04075"
     CDS             2902..3564
                     /locus_tag="PLV49_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOG98056.1"
                     /translation="MKILGICSSPRGSRSTTLRLVQAALEGAGENGAETELVDLCQLD
                     IKYCNACQTCFKTGKCVHKDDFQGLYEKILMADGLILGSPNYFHTVTAQLKTLIDRMA
                     DAVHCQLLTGKYCCSLATGGSNYDQVTSYLDSLMVNFGAFVTGSAGAEMSKGPRALEE
                     GERKARDLGRALVEDIRTKRDYIEQRKVQEGIRSHFKELVRMNKDNWEHEYEHWNSIN
                     WR"
     gene            3622..>4220
                     /locus_tag="PLV49_04080"
     CDS             3622..>4220
                     /locus_tag="PLV49_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HOG98057.1"
                     /translation="MLTVDEGSAAVRLARRALAHYMETKEALAPEGMPPVFSQKRGVF
                     VTLHEDGDLRGCIGYPRPVLPLGRAIVDSAINAGFRDPRFPGLRSGELKRIELEVTIL
                     TEPQACTGPKKDLPERIQVGRDGLIVSKGPFTGLLLPQVAIEWGFDSLEFLGQTCVKA
                     GLPADAWLDEDTLIEHFEAQIFAEVAPEREVFEKSFTESP"
BASE COUNT          889 a         1175 c         1242 g          914 t
ORIGIN      
        1 gggcgacaga ggctctggtc accccagcgc tcatggttat gatggccttc tccctgaccg
       61 aggtcgatct gggaagagat atcaaatcct cgggctctct gaaaagcgga cttctcggca
      121 tcatcataaa ctacggcctt ttgagcggat tgattctgct cctgagctac agtctgcccg
      181 acgaatcgct caggaatggc tttgtcatca tggccgcggt cccgccggca gtggccgtac
      241 tgcccatgac ccgcatcctg aaaggggaca tgagcctctc ttttgccgga gagatacttt
      301 cctacctggc ctcgctcctg ctgatgcccc tgatcatcta cctcttcgtt caccagacgg
      361 gaataagccc catctatctg ctccagatct ccattgtatt gatcctcatc cctgctcttg
      421 cctccaggct ggtacgactg attccgatca accccgtcct tcccataaac ctgggcttct
      481 tcctggtcac atatactgtg atcggcctga accagggagc cctctggacg gatgggtgga
      541 gcgtggcctg gatatccata gcccgcacct tcgcagtggg attggcagtg tttatcctgg
      601 ccagatccgc tggcttgaag agcagccagg ccatctccct tacccttctg ggctccttca
      661 agaacctggg acttgcagca gcggtatccc tgctccttgt cggtccggca gcagggctcc
      721 cggcggcatt ctgcgtcctc gcggagaccg cttttttcat acttctctcc ttcgcgagat
      781 cacggtatcc ttaaataagg taaaaagggt gatctgattg ggagatatct tgtctatcaa
      841 ggatgatgcc gcctatctga ggtcgatcta catctatgtc gccctctcag tattcctctt
      901 cgctctcact gccgtcatgg gctattatac tgctcagttt gatccagagt tcgccgccag
      961 ctggacagag gagctggaga tgctggcatg gatactggag cagccgccac tcatgataat
     1021 gataatcata ttcctcaaaa atctcctcgc ttctgccacg gctatgctct taggattggg
     1081 gctggggatc gttcccatga tagtcgccac caccaatggc tttcttctgg gaatcgtcgg
     1141 ctatagcgcg gttgaacaga agggatggct ctatctggcg gcaggcattc tgcctcatgg
     1201 aatcatcgag cttccagccg tcctcttgag tatcgccatt ggcttgcggc tgggccatat
     1261 gctagcccta tccctattaa aagagagctc agacctttcc ggagagatgc gtgtagcaat
     1321 tcatttcctc ctccgctgga taatgcccct gctccttttg gccgcggcca ttgaaacttt
     1381 tatcaccccc tttatcatat cgagggtcgc atgacatatc cagatactaa gaacaaaggc
     1441 aaaggggaat ccggcgggca gagcgccaag ctttccccca aagaggactt cagccagtgg
     1501 taccatgagc tgctgatgac aggggagatc attgataacc gctaccctgt aaaggggatg
     1561 tgcgtctggt tccccttcgg cttcgccatc aagaagaacg tctacggcat catccggcag
     1621 cttctggatc ccgatcatca ggagaccctc tttcccctga tgataccgga gaacgagttc
     1681 atgaaggagg cccagcatat caaaggcttc gaggaggagg tctactgggt gacctgcggc
     1741 gggaccaccc ctctggaggt caagctcgcc ctccgtccta ccagcgagac ggccatatac
     1801 cccatgttca agctctggat aaggtcgcat gccgacatgc ctctgcgcat ctaccagata
     1861 gtgaatacct tccgctatga gaccaagcat acccgccctc tgatacgtct gcgggagatc
     1921 acctccttca aagaggcgca taccgtacat gctacctggg aggaggcggc agagcaggtg
     1981 gaggtggcga tcaaaaggta ctcggagttc tatcgccgcc tggccatacc cttcctggtg
     2041 agccgccggc ccagctggga caagttccct ggtgcggatt ataccacagc catcgatgtg
     2101 atcatgcccg atggcaagac gctgcaggtg gggaccgccc atcatctggg ctccaccttt
     2161 gccaagacct atgagataac ctatgaggca gaggatggcg agcagaagct ggtcaatcag
     2221 acctgctacg gcatctctga gcgctgtatt gccgccctca tatccgtgca cggggacgat
     2281 aaggggctgg cattgccctg ggaggtggct cccgtccagg tggtcattgt gcccatcctc
     2341 ctcggcgaca aggagaaggt gctggaggtc tgcagggatc tgttgaggat cctgcagcag
     2401 gcaggcatcc gggtggagct ggacaccagc gacgagcggc ccggtgccaa gttctacaag
     2461 tgggagatga aaggggttcc catccgcctg gaggtcggcc ccagggatat tgagaaggga
     2521 gtggtgacgg tggtgcgccg ggatgctgag aagctcaccc tgcccaggga agggctggcc
     2581 gacgccctga tcggggaggc ggagaagctt caggcagcgc tttatcagag ggcagaggca
     2641 tttgccaatt cccgcatccg ggaggtggca agcatggatg aagcccgagc ccagactgag
     2701 gcaggggtgg ctctagtccc ctggtgcggt gaggaggagt gcggccatca gcttgaggac
     2761 caggttggag cgaacatgct tggcgagcct cagtacaaga gcttctcacc agcagcctgc
     2821 gccgtctgcg gcaaattgac cgacaagcgc acatacatgg ccagacaata ctgatccgat
     2881 tgcctgggag gatgagagag gatgaagata ctgggaattt gctcgagccc tagaggctcg
     2941 cggagcacca ctctgcggct ggttcaggct gctttagagg gtgcaggaga gaacggggca
     3001 gagactgagc tggtcgacct gtgccagctt gatatcaagt actgcaatgc ctgtcagacc
     3061 tgctttaaaa ccggcaagtg cgtgcataaa gacgactttc aggggctgta tgagaagatc
     3121 cttatggcgg acggtctgat attgggctcg cccaactact ttcatactgt gaccgcccag
     3181 ctgaagacgc taatcgaccg gatggctgat gccgttcact gccagctcct aaccggcaag
     3241 tactgctgca gcctggccac cggcggcagc aactatgacc aggtcacaag ctacctggac
     3301 agcttgatgg tcaacttcgg cgcttttgtt accggcagtg caggagctga gatgagcaaa
     3361 ggccctcgtg ccctggagga gggggagagg aaggcccgcg atctgggccg ggccctggtc
     3421 gaggatattc gcaccaagcg cgactacatc gagcagagga aggtgcagga aggcatccgc
     3481 tctcacttca aagagctggt gcgcatgaac aaggataact gggagcacga gtacgagcac
     3541 tggaacagca tcaactggcg ctagagtggg cgagctccaa cccgcataac tctttaatat
     3601 cttctaggcc tattaataat catgctgaca gtggatgagg gaagtgcagc cgtccgcctg
     3661 gccaggcgcg ccctggcaca ttatatggaa acgaaggagg ccctggctcc agaagggatg
     3721 ccgcctgtct tttcccagaa gcgtggcgtc tttgtaactt tgcatgagga tggggatctg
     3781 cggggatgca taggctaccc tcggccggtc ttgccccttg gtcgggccat tgtggattca
     3841 gcgatcaatg ccggctttcg cgatccgcgt ttccctggcc tgcggtcggg cgagctgaag
     3901 aggattgagc tggaggtcac catcctcact gagccccagg cctgcaccgg gcccaagaag
     3961 gacctgcctg agaggattca ggtgggaagg gacggcctca tcgtctccaa agggccgttc
     4021 accggtctgt tgctgcccca ggtggcgatt gaatggggtt ttgattccct ggagttttta
     4081 ggtcagacct gcgtcaaagc gggcctgccg gcggatgcct ggctggatga ggacactctg
     4141 atagagcact tcgaggcgca gatcttcgcc gaggtggcgc cggagaggga ggtatttgag
     4201 aagagcttca ctgagagtcc
//
LOCUS       DAOSGP010000104         4120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_19648_length_4120_cov_19.958180, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000104 DAOSGP010000000
VERSION     DAOSGP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19648_length_4120_cov_19.958180"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..116)
                     /locus_tag="PLV49_04085"
     tRNA            complement(42..116)
                     /locus_tag="PLV49_04085"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78..80),aa:Glu,seq:ctc)
     gene            complement(145..819)
                     /gene="nth"
                     /locus_tag="PLV49_04090"
     CDS             complement(145..819)
                     /gene="nth"
                     /locus_tag="PLV49_04090"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOG98058.1"
                     /translation="MAKQKVGDKVLACIRILEEEYGVPRQDHIDPLDLLVMTILSQNT
                     SDTNSLRAFANLKRDYGNYESLLLAPTEEVADCIREGGLANIKALRIQEVLLSIKRDR
                     GAMDIGFLEGMDKDEAMSYLLDLPGVGPKTASIVLLFAFGMPFMPVDTHVFRVSRRLG
                     LVTENLSPEKAQKALERIVPPECYHSFHLNLIRHGRQICRARGPKHEECALKECCDCF
                     LQGQKP"
     gene            911..2155
                     /locus_tag="PLV49_04095"
     CDS             911..2155
                     /locus_tag="PLV49_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOG98059.1"
                     /translation="MDFVDIYPFLVAMLIGALIGTERQRRLAEEKVRGVAGLRTFILI
                     SLLGALSATLAAQYGPLFAVGALSSFIILVAVGYASAVSSLGRIDLTAAVAAVVAFVL
                     GMLANDPDKIALAVSLAIITTWILATRTVTHRYVEALNETDLLDTLKMGIIALVIYPI
                     LPLEAVDPWRVINLRQIWLFVILVSLIGYTGYILIRILGAERGLTLTGILGGLASSIA
                     VTSSLAEESKINPQLLSSAVFATAIASSTVFPRVFFIAAIVNKEVLPDLFIPLLTMTA
                     VGVALAYISHRKSPPQGGEVKVSDPFRILPALKLGSLFALVLIISRLAGLYFGDTGIY
                     AASILSGLVDVDAITLSMATLARSTLTPSVAATSITLAVIANTLVKLGMAYLLGSREF
                     GNRTAAILLPMALAGILSTLLI"
     gene            complement(2192..2265)
                     /locus_tag="PLV49_04100"
     tRNA            complement(2192..2265)
                     /locus_tag="PLV49_04100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2229..2231),aa:Val,seq:tac)
     gene            complement(2388..3932)
                     /locus_tag="PLV49_04105"
     CDS             complement(2388..3932)
                     /locus_tag="PLV49_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOG98060.1"
                     /translation="MDITTLVVVAVYFIGLIAIGAFASKKIKNSEDFLVAGRNLGFWT
                     FTLLVVSSICSGMTILGVAGLGYATGWPSIWEQIFVPLCAAVCILFFGSKLHAVAAKT
                     GYVTMQDYFAHRFYSPRGIRGTSAIIGVFISIIYLVGQYISISMVLSWLFKIPYQWAL
                     VIGAVIVMGYTILGGLYAIGMASLIQGMMILLGVLLVGPAVIDAAGGLTHVNTVLAEM
                     DVNMTHLWYPQVHPPYAGYAFMTPMYLVSFFFLLTFGLAAAPHVVNNVLAAREDRYFK
                     WAPLAAFVIYAVIMYLCKITGFAARSMAQEGMIAMPTGVSNPADYSFIVASEYVFPGF
                     FAPLVAVIVLSAVMSTTDRLMLTIGSYVSWDIYRQFVNKEASEKSITLLSRAAIVAST
                     VITLYLAWSNPPELLAWLIWMAIGVMLACFVTPLFAGLYWRRATREGALASMILGLVG
                     TFAFSYYAKFIAPMPMHPSMYGFVISVAAMILVSLATAKPSKKLLDDTRTGMFIRGEE
                     ERKTRS"
BASE COUNT          923 a         1234 c         1102 g          861 t
ORIGIN      
        1 aatattaatg ggcaaaatag tatcaaaaaa ttggaaattc atgctccggt tgggatttga
       61 acccaagtcg aaggagtgag agtcctacat gattggccga gctacactac cggagcgcgg
      121 atggggggtg gttgttacta tcatttaagg cttttggccc tgcagaaagc agtcgcaaca
      181 ctccttcagt gcgcactcct catgcttcgg cccacgggct cggcagatct gcctcccgtg
      241 gcggatcagg ttcaggtgaa aggagtggta gcattcgggg gggactatcc tctccagggc
      301 tttctgcgct ttctccggcg ataggttttc agtaaccagg cccagccgcc gcgagacccg
      361 gaatacgtgg gtgtctacgg gcatgaacgg catgccaaag gcaaagagca ggacgataga
      421 cgccgtcttc ggcccaacgc cgggcaagtc caggaggtag ctcatggcct cgtccttatc
      481 catcccctcc agaaagccga tatccatcgc cccccggtcc ctctttatac tgaggaggac
      541 ctcctgtatc cgcagggcct tgatattcgc cagacccccc tcccggatgc agtccgccac
      601 ctcttccgtt ggggccaaaa ggagcgactc ataattcccg tagtccctct tcagattggc
      661 gaacgcccgc aggctgttgg tatcgctggt attctgagag agtatggtca tcaccaaaag
      721 gtccagagga tcgatatgat cctgccgggg aacgccatac tcctcctcca gaatacgaat
      781 gcaagctaga accttatcgc cgaccttctg ctttgccatc cctgcggaga ctggctcctt
      841 ttgccttaaa gcttattgcg aacaaccagt cagcaaaaat ttatcaacct gtgaattaaa
      901 tagagagcaa atggattttg tggacatcta cccctttcta gtggccatgc taattggagc
      961 gctcatcggc actgagcgcc agcgccgtct ggcggaggag aaggtccggg gcgtagcagg
     1021 cctgaggacc ttcatcctga tctccctgtt aggcgccctg tccgccaccc tggccgccca
     1081 gtatggaccc ctctttgccg tcggcgctct ctcctcattt atcatcctgg tggcggtggg
     1141 ctacgcctcc gccgtcagct ccctgggaag gatagatctc acagccgccg tggccgcagt
     1201 ggttgcattc gtcctgggaa tgctggccaa tgatcctgat aagatcgccc tggccgtctc
     1261 cctggcaatc atcaccacct ggatcctggc cacccggacg gtcacccacc gctacgtgga
     1321 ggccttgaac gagacggacc tgctcgacac cctcaagatg ggaataatcg ccctggtgat
     1381 ctatcccata ctgcccctgg aggccgtcga cccctggagg gtgatcaacc tccgccagat
     1441 ctggctcttc gtgatcctgg tcagcctgat aggctacacc ggctacatcc tcatccgcat
     1501 cctgggggcg gagagaggcc ttacactgac cggcatcctg ggaggcctgg cctcgagcat
     1561 cgccgtcacc tcctccctgg cggaggagtc gaagatcaat ccccagctac tatcctccgc
     1621 cgtcttcgcc acggccatcg ccagcagcac tgtctttccc cgagtctttt tcatcgccgc
     1681 aatagtaaac aaggaagttt tgccagacct tttcataccc ctcctgacca tgaccgctgt
     1741 aggcgttgcc cttgcctaca tatctcaccg caaaagcccg ccccaggggg gcgaggtgaa
     1801 ggtctccgat cccttccgca tcctccccgc cctcaagctg ggctcgctct tcgccctggt
     1861 cctgatcatc tcccgccttg ccggcctgta ctttggggat acgggaatct atgccgccag
     1921 catactctct ggtctcgtgg atgtcgatgc catcaccctg agcatggcca ccttagcccg
     1981 gtcgaccctg acccccagcg tggctgccac ctccatcaca ctggcggtga tcgccaacac
     2041 cctggtgaaa ctgggaatgg cttatctcct ggggagcaga gaattcggca accggacagc
     2101 ggctatcctg ctgcccatgg ccctggctgg aatcctctca acgctcctga tctaaagaga
     2161 tcttgatata taatatagga aaaagaaaag atgggcccga cgcggttcga acgcgtgatc
     2221 cccgccgtgt aagggcgatg tcataaccag ctagaccacg ggccctgccc ctttactatc
     2281 tgctcgtcat attagatctt atcgcttagc tcgggcgaac aagcccggcc agctgccggg
     2341 ctgctcgcga ccttttgggc tattctgagt tttctcaacc gcataattca ggacctggtt
     2401 tttcgctcct cctctccgcg gatgaacatt cccgtccggg tatcatccag cagctttttc
     2461 gagggcttgg ccgtggccag gctcaccagg atcatcgctg ccactgagat gacaaatccg
     2521 tacatgctgg ggtgcatggg catgggggcg atgaacttgg cataatagct gaaggcgaac
     2581 gtccccacca ggcccaggat catcgaggcc agggccccct cccgggtggc ccgccgccag
     2641 tacagcccgg cgaagagggg tgtcacaaag caggccagca tcacaccgat ggccatccag
     2701 atcagccagg ccaatagctc cggtggattg gaccaggcca gatacagagt gataaccgta
     2761 gaggcgacta ttgccgcccg gctgagcagg gtgatcgact tctccgaggc ctctttattg
     2821 acgaactgcc ggtatatgtc ccagctgaca tagcttccta tggtcagcat caagcggtcg
     2881 gtggtggaca tcaccgcgga taggactatc accgccacca gaggggcgaa aaatcccggg
     2941 aagacatact ccgaggctac aatgaaagag tagtctgcag gattggagac cccggtgggc
     3001 atggcgatca tcccctcctg ggccatcgat cgggcagcaa atccggtgat cttgcataga
     3061 tacatgatca ccgcatatat gacgaaagcg gcaaggggtg cccacttgaa gtaccggtcc
     3121 tctcttgcgg ccagcacatt gtttaccaca tgaggagctg ccgccaggcc aaaggtgagc
     3181 aggaagaaga atgagaccag gtacatgggg gtcatgaagg catagccggc atagggcggg
     3241 tggacctggg gataccacag gtgagtcatg ttgacatcca tctccgccaa gacagtattg
     3301 acatgggtca atccgccggc agcatcgatc accgccggac cgacgagcag cactcccaag
     3361 agaatcatca tcccctggat caaagaggcc atcccaatgg catagagccc tcccaggatg
     3421 gtgtagccca tgacgatgac cgcaccgatc accagcgccc actgataggg tatcttgaag
     3481 agccatgaca agaccatgct gatggagatg tactggccca ccagatagat tatggagatg
     3541 aagacgccga ttatggcgga cgtgcctctg atcccccggg ggctgtagaa gcggtgggca
     3601 aaataatcct gcatggtcac ataaccggtc ttggcggcga ccgcgtgaag cttcgagccg
     3661 aaaaacagta tgcacaccgc agcgcagagg ggaacgaata tctgctccca gatgctcggc
     3721 cagccggtgg catatcccag acccgccacc cccaggatgg tcatgccgct gcatatgctg
     3781 gataccacga gaagagtgaa tgtccagaat cccaggttcc ttcccgccac caagaagtcc
     3841 tcggagtttt tgatcttctt ggaagcgaaa gctccaatgg cgatgagccc tatgaaatag
     3901 acagcaacaa caaccaaagt ggtaatatcc atatccatca ccacctcaaa gcccatatca
     3961 ggagcagaat cgagatgaat actggtacaa tgatgattcc tatgaacgtc tctgtcggca
     4021 gtcctatgat cagattattc cctccatagt gttacgtgtg agcatggttc agattgagga
     4081 tctaataaag ctaaccgttc tgaactgagg gtgttcaact
//
LOCUS       DAOSGP010000105         4113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_19689_length_4113_cov_19.267620, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000105 DAOSGP010000000
VERSION     DAOSGP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19689_length_4113_cov_19.267620"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLV49_04110"
     CDS             <1..315
                     /locus_tag="PLV49_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720450.1"
                     /note="Directs the termination of nascent peptide
                     synthesis in response to the termination codons UAA, UAG
                     and UGA; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOG98061.1"
                     /translation="AVDVLILSEDLRKTRAKIVCTNRSCDYTDSQTRSGSSEPVGTCL
                     KCSSPLTIEEEVDIVSDLSKLAEMSGAEVKIISTEFEEGAQLFRAFGGIAAILRYKTS
                     HI"
     gene            321..737
                     /locus_tag="PLV49_04115"
     CDS             321..737
                     /locus_tag="PLV49_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HOG98062.1"
                     /translation="MRTIEVTVYSAVQLTERVDRVVLAIENIFPGLIMDIRTDRIDAY
                     DGPQSLRRLHRLLRSQEILDAARSVLERGRSCNIIHFQLSKQAAYMGMVSFPPQEESL
                     GSLHIQIQATDPERVIDWLAPVTENGVAVKEIDLDV"
     gene            730..1497
                     /locus_tag="PLV49_04120"
     CDS             730..1497
                     /locus_tag="PLV49_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOG98063.1"
                     /translation="MFSFSSSKLVDRPFDWAYQLEDLGYSGWEIVNEGRQRLTEGNLP
                     EARKIVETTGLVITIHLPYSDLNLASVNQPIWEETVRQMKTCLDLASDFARLAVVHPG
                     HFSPLGMHMPDAAWLQNIKGIQEICDHASNLDMRVAVENMVNMPAILGRRPEEIEGII
                     ETVDRDNLGFIFDVGHANTNGNVEDFLRLKDMMIHIHAHDNHGERDEHLPVGNGTVPW
                     KKVLAALNGYSGRIVTESRSLEEGQRSVKRLKRLLDG"
     gene            1542..2177
                     /locus_tag="PLV49_04125"
     CDS             1542..2177
                     /locus_tag="PLV49_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translin family protein"
                     /protein_id="HOG98064.1"
                     /translation="MPGLSTFENISDGLLRSFDDKDRAREEALSLSRRVIRLCSSSIR
                     SMHRSEIPAAKRLVDEAAEELEKIRELLENHQDVRYAGFVDGAEQEYAEAISVYSIIT
                     RNEILAPAEVGVELANYLAGLGDVCGELRRHILDLIRSGMAKDGEYFLEVMEEIYYLL
                     MLFDYPDAITRGLRRKSDLARSMLERTRGDLTNALEISKMESLFQNKLKKP"
     gene            2203..3813
                     /gene="sepS"
                     /locus_tag="PLV49_04130"
     CDS             2203..3813
                     /gene="sepS"
                     /locus_tag="PLV49_04130"
                     /EC_number="6.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043816 - phosphoserine-tRNA(Cys) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HOG98065.1"
                     /translation="MKFDPNQIREAAREDFDKTWQEGREYLGRPSLNERYPRNSYSFG
                     SVHPIFDTVQKLREAYIRLGFNEAMNPVMVEAQDVYRQFGSEALAVLDRCFYLAGLPR
                     PDIGISDERIAQINALLGRELSAEEVEALRQILHSYKKGKVEGDDLVGEIAHALSAHD
                     ALIGTVLEKVFPEFKGLKAEATSRTLRSHMTSGWFLSLSNLHYRQRLPIRLFSVDRCF
                     RREQAEDAARLMSYYSASCVIMDEDVSIEDGKAVADGLLSQFGFQKFQFRPDDKRSKY
                     YVPGTQIEVYAYHPGLVGSATKYSSGWVEVATFGIYSPTALSQYDIPYPVMNLGLGVE
                     RIAMILHNSQDVRALSYPQFQANWELTAREMAQMIAVEMTPSTPEGQAIAESVIGVCL
                     EHGDAASPCEFPAWEGELFGRHIQVWVVEPEENTKLCGPAALNEIVVHKQNVMGIPRT
                     SRWEEAFNEGVTTGIRFIDSFAALSAREIEVAAMNGQESETRARIVRSPADINIKIHP
                     ALERYITSYKHKIDLRGPVFTTVKSKLL"
     gene            3866..>4113
                     /locus_tag="PLV49_04135"
     CDS             3866..>4113
                     /locus_tag="PLV49_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HOG98066.1"
                     /translation="MKDASSNSAAKKAAGEAAAELVRNGMVVGLGTGSTVAWTIKRLG
                     ERVREEDLDFCGVPTSFQAEQLAIESNIRLTSLNQHPVL"
BASE COUNT          987 a         1029 c         1226 g          871 t
ORIGIN      
        1 gcagtggatg ttcttattct ctctgaggac ctcaggaaga ccagggccaa gatcgtttgc
       61 accaatcgca gctgcgacta taccgacagc cagaccagga gcggatcatc cgagccggtc
      121 ggaacttgct tgaaatgctc cagcccgctg accattgagg aagaggtgga tatagtctcc
      181 gacctgtcca agctggcaga gatgagcggg gcagaggtca agatcatctc tacggaattt
      241 gaggaaggag cacagctctt ccgggccttt ggcggcatag ctgctatcct cagatacaag
      301 accagccaca tctaggggcg atgagaacga tcgaggtcac agtctattca gccgttcagc
      361 ttacggaaag ggtcgatagg gtagtcttgg cgattgagaa catctttccc ggtctgataa
      421 tggacattcg caccgatcgt atcgacgctt atgatggccc tcaatccttg agaagattgc
      481 atcgcctcct gagaagccag gagattctgg atgctgccag gtctgtttta gagcgaggcc
      541 gaagctgcaa tataatccat tttcagctca gcaagcaggc agcctatatg ggaatggtga
      601 gctttccccc tcaagaggag tccctgggtt cattgcatat ccagattcag gccacagatc
      661 cagagagggt catcgactgg ctggcaccag tcacggagaa tggagtggca gtcaaggaga
      721 tcgatttgga tgtttagctt ttcgtccagc aagctggtgg accggccctt tgactgggcc
      781 taccagctgg aggatctggg atatagcggc tgggagatag tcaacgaagg aaggcagagg
      841 ttgactgagg gaaatctccc cgaggccagg aagatcgtag agacgacagg actggtcatc
      901 accatccacc tgccctactc ggacctgaat ttagcctcag tcaaccaacc aatatgggag
      961 gagacggtgc gacagatgaa gacctgcctg gacctggcaa gcgattttgc ccgcctggcg
     1021 gtggttcatc ctggccactt ctcccccctg ggaatgcata tgccagacgc tgcctggctg
     1081 cagaacataa agggaatcca ggagatctgc gatcatgctt cgaatctgga catgagagtg
     1141 gcagtcgaga atatggtgaa catgcctgca atcctcggga ggcgcccgga ggagatcgaa
     1201 ggcataattg agactgtgga ccgggataac ctgggattca tcttcgatgt gggacatgcc
     1261 aataccaatg gaaatgtgga ggattttttg aggcttaagg atatgatgat ccacatccat
     1321 gcccatgaca accatggaga gagagacgag catctgccgg tgggaaatgg cactgtgccc
     1381 tggaagaagg tgcttgctgc tctgaacggc tacagtggcc ggatagtaac cgaatcgcgc
     1441 tctttggaag agggccagag atcggtcaaa aggctcaaaa ggctgcttga tggatgatat
     1501 ctgacaaaat atatctgctt taggaatgag agactgagat catgcccgga ctgtccacat
     1561 ttgagaacat ctctgatgga ttgctcagaa gcttcgatga caaagaccgg gctcgcgagg
     1621 aggctctgtc cctctcccgg agggtgatac gcctgtgctc ttcgtcaatc agatcgatgc
     1681 acagaagcga aattccggcg gcaaagaggc tggtcgatga ggcagcagaa gagctggaga
     1741 agatccggga gcttttggag aaccatcagg atgtacgcta tgcaggcttt gtggacggag
     1801 cggaacagga gtacgccgag gccatctccg tctactccat catcaccaga aatgagatcc
     1861 tcgccccggc tgaggtgggt gtggaattgg cgaactatct ggcgggtttg ggcgacgtgt
     1921 gcggtgagct tcgccgtcat attctggacc tcattcgctc cggcatggca aaagatggcg
     1981 aatacttcct ggaggtcatg gaggagatct attatctgct gatgctattc gactatccgg
     2041 atgctataac cagaggtctg cggaggaaga gcgatcttgc caggtccatg ctggaaagga
     2101 cccgagggga ccttaccaat gccctggaga tatccaaaat ggaatcatta tttcaaaata
     2161 aactcaaaaa gccttaaatc aatagatcat ggagtttaag tcatgaagtt cgatcccaat
     2221 cagatccggg aggcggcccg ggaggatttc gataagacct ggcaggaagg aagagagtac
     2281 cttggccggc cgtccttgaa tgagagatat ccgcgaaata gctattcctt cggctcagtt
     2341 catcctatct tcgataccgt tcagaagcta cgagaggcct atattcgcct cggcttcaat
     2401 gaggccatga atccggtgat ggtcgaggcc caggatgtct accgccagtt cggctccgag
     2461 gctcttgcag ttctggacag gtgcttttat ctcgccggcc ttccccggcc ggacatcggc
     2521 atatcggacg agaggatcgc ccagataaac gccctcctgg gtcgggagct ctcggcagag
     2581 gaggtggagg cactccgtca gatcctccat tcctacaaaa aggggaaggt tgagggcgac
     2641 gacctggtgg gagagatcgc tcatgccctt tctgctcacg atgctctgat cggcactgtc
     2701 ctggagaagg tgtttccgga gttcaaagga ctgaaagcag aggccaccag tcgcaccttg
     2761 agaagccata tgacaagtgg ctggtttctg tccctctcca acctccatta ccgccagagg
     2821 ctgcccatca gactcttctc tgtggaccga tgcttccgcc gggagcaggc ggaggatgcc
     2881 gcccgcctga tgagctacta ctcggccagc tgtgtgatca tggatgagga tgtatccata
     2941 gaggatggga aggcggttgc agacggcctc ctcagccagt tcggtttcca gaagttccag
     3001 ttccggccgg atgacaagag gagcaagtac tacgttcccg ggacccaaat agaggtctat
     3061 gcttatcatc cggggctggt cggctcggcc accaagtaca gcagcggctg ggttgaggtg
     3121 gctacattcg gcatctactc gcccactgcc ctgtcccaat atgatatccc ctatccggtc
     3181 atgaacctgg ggctgggagt ggagcggatc gccatgattc tgcataactc tcaggatgtg
     3241 agagctctct cctaccccca gttccaggcc aattgggagc tcactgcccg ggagatggcc
     3301 cagatgatcg ctgttgaaat gacccccagc acgccagagg ggcaggcgat agccgaatct
     3361 gtgatcgggg tgtgccttga gcatggtgat gccgcctctc cctgcgagtt tcctgcctgg
     3421 gaaggagagc tcttcggaag gcatattcag gtctgggtgg tcgagccgga ggagaacacc
     3481 aagctctgcg ggccggctgc cctaaatgag atcgtggtcc ataagcagaa cgtcatgggc
     3541 atcccccgca cctcccgctg ggaagaggcg ttcaatgagg gagtgaccac aggaattcga
     3601 ttcatcgatt cctttgctgc tctttctgcc cgtgagatcg aggtggcggc gatgaatggc
     3661 caggagtctg agaccagagc gaggattgtg agaagcccgg cggatataaa catcaagatt
     3721 cacccagctc tcgagaggta catcaccagc tacaagcata agatcgacct gcggggaccg
     3781 gtcttcacca cggtgaagag caagctgctc tgaccggatg gattgcggga attttgagag
     3841 gaaataaagg gaaactgaga ggagcatgaa ggacgcatca tccaattccg ctgccaagaa
     3901 ggcggcaggt gaggcagcgg cagagctggt taggaacggt atggtggttg gcctgggcac
     3961 gggctccact gtggcctgga cgataaagag gctgggagag agggtcagag aggaggatct
     4021 ggacttttgt ggcgttccca cctcttttca ggccgaacag ctggccatag aatcaaatat
     4081 caggctcacc agcttaaacc agcatccagt tct
//
LOCUS       DAOSGP010000106         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_19930_length_4077_cov_19.551716, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000106 DAOSGP010000000
VERSION     DAOSGP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19930_length_4077_cov_19.551716"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLV49_04140"
     CDS             complement(<1..227)
                     /locus_tag="PLV49_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720450.1"
                     /note="Directs the termination of nascent peptide
                     synthesis in response to the termination codons UAA, UAG
                     and UGA; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOG98067.1"
                     /translation="MEFTAQERYEFKRLLDGLRSKKGRGTELISLYIPPDKQISDVTS
                     QLREEYGQASNIKSRVTRLSVQGSLESAMSRL"
     gene            513..>1831
                     /locus_tag="PLV49_04145"
     CDS             513..>1831
                     /locus_tag="PLV49_04145"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG98068.1"
                     /translation="MDQLVVLQEIYESFSKGGDGLQKIGNKAIEGSCPELTAKLLESA
                     KNGEINEDICRQLSSCLEDIYEIKRLGDICRSTGLYSLAISAYNRALSLCSDPVLRPV
                     LQNNLGQAYARQGDLARAAFYYKKSACCFEKSEDLLGLAHVLGNLGAAYRQNRDWDRA
                     IEYCYRSLKTFEEQGDDLGIAQMTGSLGRIYADMGERDLATRYFEKSLEDFQRLGDKK
                     SAAWIMDRMGRIASDRYDWDSAQSYFSQSFGLFEEYGQTQSKGIVLSNMGRMQLEKGD
                     ASSARESLEKAIHLISRNAQPGYQNALSSLAAAYSTLANGSLEEAEDNEDLGLGSGRL
                     QRQEASKNFGRASDRFLELASSLPKMQADIKTKSEIAKCRSYLARISGYTSDQEAVEI
                     AQKALSSLDTAAENAAETKKAGIRGLAKAVSGMREARSIGLLGDEPWR"
     assembly_gap    1832..1931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1932..2512
                     /locus_tag="PLV49_04150"
     CDS             <1932..2512
                     /locus_tag="PLV49_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98069.1"
                     /translation="QPDIIDGMDELIESDLEEDFDGLPDLEISEDGAQARNMILTDGT
                     VEPSELYVSEIMHPEDSWLVPVMASTPCKKENPIGFNSWRQAQFYGSKGFDHLEKPED
                     DIEKIDGVIDTGLIEELNRHDLGGMQGGEWPERGLEDSGSDFREAVPEFKESTSSYGS
                     GLFSQEKAVMLIKAMSVLVIILLAVEAVLYLI"
     gene            2537..3133
                     /locus_tag="PLV49_04155"
     CDS             2537..3133
                     /locus_tag="PLV49_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HOG98070.1"
                     /translation="MKKKQLEMTLERLEGFSEPSFIREQYATPASVAAELLFLAALRN
                     DLGTVCDLGCGTGVLAIGAALMGARTVGVEIDKDALATARKNAENLGVDVDFIRADVR
                     KLELEGIDTVIMNPPFGAQKASEGDRAFLSTAQRIARNIYTLHNRGSHDFIKKYIFPN
                     VVQEVYCIPFPLKRCFEFHRKDIKVIEVELYRITCQHS"
     gene            3272..3832
                     /locus_tag="PLV49_04160"
     CDS             3272..3832
                     /locus_tag="PLV49_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Csl4"
                     /protein_id="HOG98071.1"
                     /translation="MMEDSFVLPGDTVGSLEEFVPGDYTYAREGMIFATTAGLVKVDP
                     KTRSASVIPKTNAPVKLNHGDIVVGEVIDLKDSLVIVSLAFKKGYEDRPISDEEATIH
                     ISNVKNSYVKDLRSLFSMRDILKAKIVDERQMRLSTGDEDLGVIKAYCNRCLTGLMRK
                     EGKLICPNCGNNETRKMSSAYGLGVV"
     gene            3833..>4077
                     /locus_tag="PLV49_04165"
     CDS             3833..>4077
                     /locus_tag="PLV49_04165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HOG98072.1"
                     /translation="MNLKIISKTENEAVIEFVGEGHTILNLLRTELLKDKRVLMATYD
                     TKFPVMDNPVFRLVTNGADPIIALKEATSRIIDQCDE"
BASE COUNT         1053 a          907 c         1100 g          917 t
ORIGIN      
        1 agcctggaca tggctgactc gagggagccc tggacgctaa gccttgtcac tctcgacttt
       61 atattggatg cttgcccgta ttcctcccgc aactggctgg taacatcaga tatctgctta
      121 tcaggaggga tatacaggga aatgagctct gtgcccctgc ccttcttgct ccggaggccg
      181 tcaagaagac gcttaaattc atacctttcc tgcgctgtaa actccattct agccgatctc
      241 caaaattgct cctggatcaa ggtataaaag gcttcttcaa aaggatttcg gatgataatc
      301 cttgatttgg aggaaaattt ttttcaattc tggattttca aagggaggca ccaattatta
      361 ctatcttaat aatactagtc agatttggct gaaataattg atcaatttat caaattttcc
      421 taattaatca ggagtcttaa aatcagtcaa tagaaatggc agaatttgat ctattcaaaa
      481 actatataaa gatataagtt atcttataat ccatggatca attagtcgtt ctccaggaga
      541 tctatgaatc gttttccaag ggaggggatg gcctgcaaaa gatcggcaac aaggctatcg
      601 aggggagctg tccggagctg accgccaagc tgctggaaag cgccaagaat ggcgagatca
      661 atgaggatat ctgtcgccag ctttccagct gccttgagga tatctacgag atcaagcggc
      721 tgggcgatat ctgtcgcagc actggattgt attcccttgc cataagtgcc tataaccggg
      781 ccctatctct ctgcagcgat ccggttttga ggccggttct tcaaaacaat ctcggtcaag
      841 cctatgcccg ccagggagat ttagcccgag cagccttcta ctacaaaaaa tctgcttgtt
      901 gctttgaaaa atcagaggat ctcctaggcc ttgcccatgt cctgggaaac ctgggagctg
      961 cctaccgtca gaaccgggat tgggatcggg ccatagagta ctgctaccgc agtttgaaga
     1021 cctttgagga acagggtgat gatctcggca ttgcccagat gactgggagc cttggaagga
     1081 tctatgcgga catgggggag agggatctgg caactcgata ctttgagaaa agcctcgagg
     1141 atttccagag gctcggcgac aagaagagcg ctgcctggat tatggatagg atgggacgga
     1201 tcgccagcga tagatacgac tgggatagcg ctcagagcta cttcagccag agcttcggcc
     1261 tgttcgaaga gtatggccag acccaaagca aagggatcgt cctctctaat atgggacgga
     1321 tgcagctgga gaagggagat gcatcttctg ctcgcgaatc tttggaaaag gccattcatc
     1381 tcataagccg caatgcccaa cctggttatc agaatgctct gtcctcactg gccgcggctt
     1441 acagcaccct ggcaaatggc tccctggaag aggcagagga taatgaggac ctgggtctgg
     1501 gctcaggccg gctgcaaagg caagaggcat ccaagaattt cggccgggca tcagatcgct
     1561 tcctagagct agcttcctct cttcccaaga tgcaggcgga tataaaaaca aagtcagaga
     1621 ttgctaaatg tcggtcctat cttgccagga tatccggata cacctctgac caggaggcag
     1681 tggaaattgc acaaaaggct ctgtcgtcgc tggacacggc tgcagagaat gcagctgaaa
     1741 ctaaaaaggc cggaatcaga ggtctggcga aggccgtatc cgggatgaga gaggcaaggt
     1801 cgataggcct gctgggagac gagccctggc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn ntacagccag atatcatcga tgggatggat gaactgatcg agtccgatct
     1981 ggaagaggat ttcgatgggc tgccggatct ggagatctca gaggatgggg cgcaggccag
     2041 gaacatgatt ttgactgacg gcaccgtgga gccctctgag ctttatgtct cagagataat
     2101 gcatccagaa gacagctggc tcgttcctgt catggctagt actccctgca agaaagaaaa
     2161 tccaattggc tttaattcct ggaggcaggc gcaattttat ggatccaagg gctttgacca
     2221 cctggagaag ccagaggatg atatcgaaaa gatcgatggg gtcatagata ctgggctgat
     2281 agaagagctt aaccgccatg atcttggcgg aatgcagggg ggggagtggc cggagagagg
     2341 gcttgaggat tctggttcag actttcgcga ggcggtaccg gagtttaagg aatcaacctc
     2401 cagctatggc agcggtctat tcagccagga aaaggcggta atgctgatca aggccatgag
     2461 cgtcctggtg atcattctct tggcggttga ggcggtgcta tatttgatat aggctgagcg
     2521 ggaatcctct atcgggatga aaaaaaagca gcttgagatg accctggaac gactggaggg
     2581 cttttctgag ccctcattta ttagagagca gtatgcaacg cccgcctcgg tggcggcgga
     2641 gctgctcttc ttagctgctc tgcgcaatga tctgggtacg gtatgcgacc tgggatgcgg
     2701 gacgggcgtt cttgccatcg gcgcggcgct catgggtgca aggacggtgg gagtggagat
     2761 cgataaagat gccctggcca cggcgagaaa aaatgcagaa aatctgggcg tagatgtaga
     2821 cttcatccgg gcggacgtga gaaagctgga gcttgaaggc atagatacag tgatcatgaa
     2881 tccccccttt ggagctcaga aggccagcga gggcgatcgc gctttcctct ccactgctca
     2941 gaggatcgct cggaatattt ataccctgca caaccgggga agccatgatt tcattaaaaa
     3001 atatatattt cccaatgtag tgcaggaggt ctattgcatc ccatttcctt tgaagagatg
     3061 ctttgagttt cacagaaagg acataaaggt cattgaggtt gagctataca gaataacctg
     3121 ccagcattct tagaggagcc aagcttttga gaccaagcct ttataccagg ctttagatac
     3181 tagtaatttc taattgagcc atagcgatga aaaaagagac ggaaatgatg cggcgattct
     3241 cgagccgtat attgaattta cagaggaata aatgatggaa gatagctttg tgcttcccgg
     3301 cgatacggta gggtctttgg aggagttcgt tcctggagac tacacctatg ccagggaagg
     3361 aatgatattt gccaccactg ccggcctggt taaggtcgat cccaagacca ggtctgccag
     3421 tgtcataccc aagaccaacg ccccagtcaa gctcaaccac ggcgatatag tagtgggcga
     3481 ggtaatcgat ctcaaagaca gcctggtgat agtctccctg gccttcaaga aaggctatga
     3541 ggaccggcca atctcagatg aggaggccac cattcacata tccaatgtga agaactccta
     3601 tgtcaaggat ctgcgctcgc tcttttccat gcgcgacatc ctcaaggcaa agatcgtcga
     3661 tgagcggcag atgaggctct cgactggcga cgaggatctg ggagtgataa aggcttactg
     3721 caaccgctgt cttaccggcc tgatgcgaaa ggagggaaag ctcatctgcc ccaattgcgg
     3781 caacaacgag acgcgaaaga tgagctccgc ctatgggctg ggggtagtgt agatgaacct
     3841 gaagataata tccaagaccg agaatgaggc agtcattgag ttcgtgggag agggtcatac
     3901 cattctcaac ttgctccgaa cggagctgtt gaaggacaag agagtcctca tggcaacata
     3961 tgacacaaag tttccggtaa tggacaaccc cgtcttccgc ctcgtaacca atggggccga
     4021 tcccattatt gctctgaaag aggccacatc cagaataata gatcagtgcg atgagtt
//
LOCUS       DAOSGP010000107         4061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_20051_length_4061_cov_18.718672, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000107 DAOSGP010000000
VERSION     DAOSGP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20051_length_4061_cov_18.718672"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLV49_04170"
     CDS             complement(<1..359)
                     /locus_tag="PLV49_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOG98073.1"
                     /translation="MASIVVGKEFSEILEKIERSEAVVLTAEEVSRLVEEKDRSSLEE
                     VDVVTTATRAVMSGTYAILSFPVAEPGSFQRAKNVWLNGISAQVGPCPNEKLGVLDMV
                     IFGTAHSRDRPDYGGGHL"
     gene            682..1689
                     /locus_tag="PLV49_04175"
     CDS             682..1689
                     /locus_tag="PLV49_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG98074.1"
                     /translation="MRKFVLIATLAAVVVLTLGCISPPEKTESTNLTDKSAGITTLRI
                     GYQPSTHQIAEMVASEMGWWADDLSPFGITEVKEFEFPTGVPEMQAMVAGELDIAYVG
                     TAPPISAISAGLPAKIVAAVNIKGSDLVVRPDLAYSGPESLEGLSIGTFPPGSIQDTV
                     MKKWLTDNGVEVSEVDIKAMDPGPAMSALSAGRIDGVFLPHPAPSIVELNGKGKVVVA
                     SGEMWPDHACCSLVVTDKLIREQPELVEQIIKTHIKATEYINTHPKEAAEIYSRKTNA
                     NITEIEHSIENWDGEWVSDPNLQIPSTVEYARVDYEMKYTQRQLTEEDLFDTSFYERV
                     S"
     gene            1763..2485
                     /locus_tag="PLV49_04180"
     CDS             1763..2485
                     /locus_tag="PLV49_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98075.1"
                     /translation="MLIALWETAAFLIDDRLILPSFSQVVFAFMENWRSILLDDLPVS
                     ALHFAIGMAGGLLIALPIGMIMGWFKFLDSIFDPIVEILRPIPPLAWIPFAIIWFGIT
                     NTAAGFIIFIGAVFPILTNTYMGFKSLPRVYVESAKVLGATKDIDLIRYVALPYSLPS
                     IAAGIRIAMGIAWMCLVAAEMFGVSKNGLGFKIWDSYGHYRMDEVFMYMIVLGLLGLA
                     IDRTFRYVVEERMLKWQVGLTQ"
     gene            2644..3834
                     /locus_tag="PLV49_04185"
     CDS             2644..3834
                     /locus_tag="PLV49_04185"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOG98076.1"
                     /translation="MKRNIKVERLNQRPLEQQEIELVERKGIGHPDSVADGLAESISR
                     ALCQEYLDRFGAVLHHNTDKTQIVAGRSKPAFGGGEVICPLYILLTGRATRVFKEEEI
                     PVDTIAIKAARKLLVESLSNLDVNNHIILDAKIGMGSSDLRDVFGRKVPSANDTSFGV
                     GYAPLSQTENIVYNTERELMNLKKSIPAIGEDIKVMGMRQGDEINLTIACALVDRYVA
                     NMKEYADTKREIVEHVIEFARQFTSRKVAAQMNVADNIEEGSVYITVTGTSAEMGDDG
                     AVGRGNRANGLITPNRPMSLEATCGKNPINHVGKIYNLLSIEAAKQIAAEVQGIEEVY
                     IKILSQIGKPIDEPHIASVQIVPKEGVDIKSLQDGATEILDDWLGSIPKLQQMLFRGE
                     LSTY"
     gene            complement(3882..>4061)
                     /locus_tag="PLV49_04190"
     CDS             complement(3882..>4061)
                     /locus_tag="PLV49_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOG98077.1"
                     /translation="QSGGGERMAEEMNVRFLGSIPIDPQVCSLGDAGQTFVEGDMPAA
                     DVFRLIVERLKNILR"
BASE COUNT          985 a         1067 c         1075 g          934 t
ORIGIN      
        1 agatggcctc cgccataatc cggccggtcg cggctgtggg cagtgccgaa tatgaccata
       61 tccaaaaccc ccagcttctc attgggacag ggtcccacct gggcagatat gccgttcagc
      121 cagacgttct tcgccctctg gaaggatccc ggctcggcta ccggaaagga gagaatggca
      181 tatgtcccgc tcatcaccgc ccgggtagca gtggttacca cgtccacctc ttcgaggctt
      241 gacctatcct tctcctccac caggcgggat acctcttctg cggtgaggac aaccgcctcg
      301 ctccgctcta tcttctccag tatttcggag aattcttttc cgaccactat gcttgccatc
      361 cttcacctgc gcgacctgct ctgccgctct ccttgtctgg cctgctcgct tgggatgctc
      421 gaacggagtt ccttgcatac catactattt cattatccgc atttcccttt caatctgccg
      481 attgtcccct cattctttgg ccattttgta gtatatgtcc actcagttgt ccagatgggg
      541 aaattgctgt agcatgaccg gaaaatgctt tataccgatc tttccccaga tgaccataag
      601 tcggtataat caaggtacag ttaggtaggc ataggtagac attgacggtt taggcaggtt
      661 agataggtat aggtgatcgc attgaggaaa ttcgtgttga tagccaccct ggcagcagtg
      721 gtggtactaa ccttgggttg catatcccct cctgagaaga ccgaaagcac taatctcaca
      781 gataaatccg caggaattac caccctgcgc ataggctacc agcccagcac ccatcagatt
      841 gccgagatgg ttgcctctga gatgggctgg tgggcggatg atctttcgcc ttttggtata
      901 actgaggtta aagagtttga gtttcccaca ggagttccgg agatgcaggc catggtcgca
      961 ggagagctgg atattgcata cgtcggaaca gcgccgccca tttcagccat atctgcagga
     1021 cttccggcta agatcgtggc cgcagtcaac atcaagggtt cggatctggt tgtccgcccg
     1081 gacttggcct acagcggacc ggaatccctg gaaggcctct ccataggcac attcccgccc
     1141 gggtcaattc aggacactgt aatgaagaag tggcttacgg ataatggagt tgaagtctca
     1201 gaagtggata tcaaagccat ggacccagga cctgctatga gcgctctatc cgcaggcaga
     1261 atagatggag tattccttcc ccatccagcc ccatccattg tggaactgaa cggcaagggc
     1321 aaggtggtgg tggcatcagg cgagatgtgg ccggaccacg cctgctgcag tctggtggtc
     1381 acagacaagc tcatccgaga gcagcccgaa ctggtagagc agatcataaa gacccatata
     1441 aaagcgacag agtacatcaa tactcatccg aaagaggccg cagagatcta ttccagaaaa
     1501 accaatgcga atatcactga aattgagcat tccattgaga actgggacgg cgagtgggtc
     1561 agcgatccga atctccaaat accctcaacg gtagaatatg caagagtcga ctatgagatg
     1621 aagtatactc aaaggcagct gacagaggaa gatctcttcg ataccagctt ctacgagagg
     1681 gtgagctgaa ctgagaaaag ctagtgggag caggatctcg cgtgtcctga gagaccaggg
     1741 cattcccgtc ctttccattt ttatgttgat tgctctctgg gagacggcag catttctcat
     1801 tgatgacaga ttgattctgc ccagcttctc ccaggttgtt tttgccttta tggaaaactg
     1861 gcggtctatt cttttggatg acctgccagt cagcgcactt catttcgcca taggcatggc
     1921 gggcggtctt ttaattgccc ttcccattgg aatgataatg ggatggttta agtttctgga
     1981 cagcattttt gatccaatcg tggagatact gcgccccata ccgccactgg cctggatccc
     2041 atttgccatc atctggtttg gcattaccaa tactgcagca ggatttatca tattcatcgg
     2101 ggctgtgttt cccatattga ccaacaccta catgggcttc aagagccttc cccgtgtgta
     2161 tgttgagtca gcaaaggttc tgggtgcaac caaagatatc gatctgatca ggtatgttgc
     2221 tctgccctac tctctgccct ccattgcagc aggcataagg attgccatgg gaattgcctg
     2281 gatgtgcctt gtcgccgccg agatgttcgg ggtcagcaag aatggcctgg gattcaagat
     2341 ctgggactct tatggccact accggatgga cgaggtattc atgtacatga tcgtcctggg
     2401 tctcctgggc ctggcaatag atcgtacctt ccgctatgtg gtggaggaga gaatgctcaa
     2461 gtggcaggtg ggcctgactc agtaagccgg agccagggga taggggccac ctcctccgtc
     2521 tcgcccattt gggcttattt tccaattggg ctgcagtgca aagcattaat atgacagtgc
     2581 acctctttgt tttgtggaac tgccttaaaa gcttgtcaat gaaacggcat tcgaggaaat
     2641 gacatgaaga gaaacatcaa ggttgaacgc ctcaatcaaa ggcctttgga gcagcaggag
     2701 atagagctgg tagagcgcaa gggaataggc catccggaca gcgtagcaga cggcctcgca
     2761 gagtccataa gtcgggctct atgccaggaa taccttgata gatttggagc ggtgctgcac
     2821 cacaacactg ataaaaccca gatagtggct ggaaggtcta aaccggcatt tggaggcggc
     2881 gaggtcatct gcccgctgta tattctgctc accggcaggg ccacgagggt cttcaaggag
     2941 gaggagatcc cggtggacac cattgccatc aaagcggcgc gcaagctcct tgttgagtcc
     3001 ctctccaatt tggatgtcaa caaccacatc atcctcgatg cgaagatagg tatgggctcc
     3061 tctgatctgc gcgacgtctt cggtcggaag gtcccctccg ccaatgatac ctcctttggc
     3121 gttggctatg cccccctctc ccagacggag aatatcgtct ataacactga gcgagagctc
     3181 atgaatctca agaagagcat tcccgccatt ggcgaggaca tcaaggtcat gggcatgcgc
     3241 cagggagatg aaatcaacct caccatcgcc tgcgccctgg tggacaggta tgtggctaac
     3301 atgaaagagt atgcagacac gaagagggaa attgtcgagc atgtgattga gttcgccagg
     3361 cagttcacct cccgcaaggt agcagcccag atgaatgtgg cggacaacat cgaagaggga
     3421 tcggtctata tcacagtcac tggaacctca gcggagatgg gagacgacgg tgcagtgggc
     3481 cggggcaacc gggccaatgg cctcatcacc cccaaccggc ccatgagcct ggaggcgacc
     3541 tgcggaaaga accccatcaa ccacgttgga aagatctata acctcctgtc catcgaggca
     3601 gcaaaacaga tcgccgccga ggtccaggga atcgaagagg tctacatcaa gatcctatca
     3661 cagataggaa agcccatcga cgagccccat atcgccagcg tgcagatcgt ccccaaggaa
     3721 ggagtggaca taaagtcatt gcaggatggg gccaccgaga ttctggacga ctggctgggg
     3781 agtataccaa agctgcagca gatgctcttc cggggagagc tcagcacata ctgagatctc
     3841 aacaaatttt ttaaaaaaaa ggtttagccc caaggcagta gctatctcag gatatttttc
     3901 agcctctcta caataaggcg gaagacatct gcggctggca tatctccctc tacgaaggtc
     3961 tggccggcat cgcccagtga gcagacctgg ggatcaatgg gaatggatcc cagaaatctc
     4021 acattcatct cctctgccat tctctccccg ccgccgctct g
//
LOCUS       DAOSGP010000108         4015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_20385_length_4015_cov_18.020707, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000108 DAOSGP010000000
VERSION     DAOSGP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20385_length_4015_cov_18.020707"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLV49_04195"
     CDS             <1..829
                     /locus_tag="PLV49_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG98078.1"
                     /translation="YRGFLDAIRRSGNRDILTTLDKVNIKVREGEVYGLLGPNGAGKS
                     TLIKILCTLILPSEGEAYVNGYDVVKEGQQARESLGFVTTDERSFYWRLSGRENLQFF
                     ATLYNLPQSVVRSRTEELLDVVRLKHRADEPFYSYSAGMKQQMAIARGLLNDPAVLFM
                     DEPTRSLDPGAARNLRNFIKEKIVGEKGKTIFISTHQLDEAEELCDRVAILDEGKIKA
                     NGSPLELKAGLSQGCTLGDVFSHYTGKASLDEQSTPFPVNIPRQKSRGRGMGRRRGI"
     gene            826..1632
                     /locus_tag="PLV49_04200"
     CDS             826..1632
                     /locus_tag="PLV49_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98079.1"
                     /translation="MIFEKAFWFIKKGYMLQLSYKFDFFLRLFFMLFNILTYYFIAKL
                     IGDGAAKYLEPYGGDYFSFVLIGMAFSGYLMISLRSFSESVRDEQMMGTLEAMLVTPT
                     STSSIIALSSLWNFIFASFRVLLYLVIGILLGVDMTRANVGGALLILVLTIICFSSIG
                     ILSASFIMVFKRGDPINMLLMGTSELFGGVLFPIEVFPSWMQSISHILPITYSVNGMR
                     HALLQGYSIGELAPELLSLVLFSIVLLPLSLFVFERAVLKVKAEGGLVQY"
     gene            1701..>1870
                     /gene="fpoO"
                     /locus_tag="PLV49_04205"
     CDS             1701..>1870
                     /gene="fpoO"
                     /locus_tag="PLV49_04205"
                     /inference="COORDINATES: protein motif:HMM:NF022088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420H2 dehydrogenase subunit FpoO"
                     /protein_id="HOG98080.1"
                     /translation="MADCDLCTRARPTLYPIKAPVHNLTYPEGAYKGVCDICLEHLEK
                     GWQERFGSKPEE"
     assembly_gap    1871..1970
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1971..2912
                     /locus_tag="PLV49_04210"
     CDS             <1971..2912
                     /locus_tag="PLV49_04210"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOG98081.1"
                     /translation="LEEYHRRPDPYEKEGLAAPTGMTLDDYGESLHKKAIDSGVALLD
                     GMGFAPGLSNVTLGEGIRKVGAKRAVARVGGIPCREAAARHPLQYMITWSFDHVLREY
                     MVRVRVLENGQVVEVAATSGRESFRFQECGIDEQLECAITPGMPSFLYTHNHLESFSE
                     KTVRWPGHWQAIDTLKECGLLDIAHINYAGQSFTPRDFFLNLIEPKLRPLPGDEDVCV
                     MWNTAWGREEQADYFMWTGADSKNGISAMARVTSSSAAVAARLLAQGRIKEKGIVAPE
                     EAFKDEAYSDLMRELEGRGINIVEAIRPVEATTTLNA"
     gene            2921..3706
                     /locus_tag="PLV49_04215"
     CDS             2921..3706
                     /locus_tag="PLV49_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOG98082.1"
                     /translation="MARKRSEKSLIITLLSDFGSFYPAQIKGVILSRLQGKSENIAGK
                     ITFVDIAHDIPAQDVRAGAFALLCTVRFFPPGTIHIAVVDPGVGTARRGLVVESGGQM
                     FAGPDNGLLIPAARSLGRPTAHEIALPPGASSTFHGRDVFAPAAALIAAGQPPASLGP
                     RVQPVDLDFGEAKKTARGLEATIIYVDRFGNLILNLQRLPAQDMMLKGVRLKRARTYA
                     EGRRIEPLITLGSHGFAEIAINQGNASEAFCLKAQDRIELEEI"
     gene            3708..>4015
                     /locus_tag="PLV49_04220"
     CDS             3708..>4015
                     /locus_tag="PLV49_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOG98083.1"
                     /translation="MFGIEFVPDESVLKIGYMAKLAEDAGFGNIWITDHYNNRDVWTT
                     LAVLSLLTNKISLGTGVTNPYTRNAAITASSIASINELSGGRAILGIGPGDKATFDKM
                     G"
BASE COUNT          902 a         1017 c         1144 g          852 t
ORIGIN      
        1 gtatagagga ttcctggatg ccataagaag atccggaaat agagatattc tgaccacgct
       61 ggataaagtc aacatcaaag ttcgggaggg ggaggtctat ggccttctcg gtccaaacgg
      121 agctggcaag tccactctga tcaagatact gtgcaccctt attctgccgt cagagggaga
      181 ggcatatgta aatggctacg atgtggtgaa agagggtcag caggcaaggg agtccctggg
      241 ctttgtgacc accgatgaga ggagcttcta ctggaggctc tcgggcagag agaacctgca
      301 gttttttgcc actctctaca atcttcctca gagcgtcgtc aggtcaagaa cagaggagct
      361 tttggatgtg gtccggctca aacaccgggc ggatgagccc ttctatagct actctgcggg
      421 catgaagcag cagatggcca tcgctagggg gcttttaaac gatcctgcag tcctcttcat
      481 ggacgagccg accagaagcc tggatccggg agcagcaagg aacctgcgga attttatcaa
      541 ggagaagatc gtaggagaga agggcaagac catattcatc tccacccatc agctggatga
      601 ggcggaggag ctctgcgata gagtggccat actggatgag gggaagatca aggccaatgg
      661 gagcccgttg gagctgaagg ccggtctttc ccagggttgc actctgggcg atgtcttcag
      721 ccattatacc ggcaaggcat cgctagatga gcagtcgacg ccatttccag taaacatccc
      781 ccggcagaag agcagagggc ggggaatggg caggaggagg ggaatatgat cttcgagaag
      841 gcattctggt tcatcaagaa gggctatatg ttgcagttga gctacaagtt cgacttcttc
      901 ttaaggctgt ttttcatgct cttcaatata ctgacctact acttcatagc caagctgata
      961 ggggatgggg ccgccaagta cctggagcca tatggagggg attacttctc cttcgtcctc
     1021 ataggcatgg ccttctccgg atatctgatg atctcgctcc ggagcttttc cgagagcgtg
     1081 cgagatgagc agatgatggg aactttagag gccatgctgg tgacccccac cagcacctca
     1141 agcatcatcg ccctgtcatc cctgtggaac ttcatcttcg cctccttccg ggtgctgctc
     1201 tatctggtca taggcatcct cctgggagtg gatatgaccc gggccaatgt tggaggcgcg
     1261 ctattgatcc tggtcctcac cataatatgc ttcagcagca taggaatact ttcggccagc
     1321 ttcataatgg tcttcaaaag gggagacccg atcaacatgc tcctgatggg cacctctgag
     1381 ctcttcggcg gggtcctctt tcccatcgag gtcttcccgt cctggatgca gagcatctct
     1441 cacatcctgc ccatcaccta ctcggtcaat ggcatgaggc atgctctctt gcaggggtac
     1501 tccatcggcg agctggctcc ggagttgctc agcctggtcc tgttctcgat cgtgctgctt
     1561 ccactgagcc tgtttgtgtt cgagagggcg gttttaaagg tcaaggcaga gggaggattg
     1621 gtgcagtatt aatggcgctt tcaacagaaa agttcaacaa tgagaaaaac ctacacccat
     1681 gcgtaggaga tgaatctaat atggctgatt gcgatctatg tacccgtgct cgaccaacac
     1741 tttatcccat caaggctccg gtgcataacc tgacctatcc tgagggagcc tataaaggag
     1801 tatgcgacat ctgcctggag catctggaga agggctggca ggagcgcttt gggtccaagc
     1861 ctgaagagaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctggaggagt
     1981 atcaccgccg accggatccc tatgagaagg agggtttggc agcaccaacg ggcatgaccc
     2041 tggacgatta tggcgagtca ttgcacaaaa aggctattga tagcggtgtt gcccttctgg
     2101 atggcatggg atttgcccct ggactctcca atgtcaccct gggcgaggga atcagaaagg
     2161 tgggagccaa aagggcggtg gcccgggtgg gcgggattcc ctgccgagag gcagcggctc
     2221 gccatccatt gcaatatatg atcacctggt cctttgatca tgttctgcgc gagtacatgg
     2281 tccgggtcag ggtgctggag aacggccagg tggtagaggt tgcggcaacc tcaggacggg
     2341 agagcttccg gttccaggaa tgcggaatcg atgagcagct ggagtgcgcc atcaccccgg
     2401 ggatgcccag cttcctgtac acccacaacc atctggagag cttctctgag aagacagttc
     2461 gctggccggg ccactggcag gccattgaca ccctgaaaga gtgcggcctt ttggatattg
     2521 cccatataaa ttatgccggc cagtccttca ccccccggga cttcttcttg aatttgatag
     2581 agccgaagct cagacccctg cccggtgacg aggacgtctg cgtcatgtgg aacactgcct
     2641 ggggaaggga agagcaggcc gactacttca tgtggacggg cgcagacagc aaaaacggca
     2701 tatctgccat ggccagggtg accagctcct ccgctgcggt tgccgccagg cttctggccc
     2761 agggcaggat caaggagaag gggattgtcg ctccggagga ggccttcaaa gatgaagcct
     2821 atagcgatct catgcgggag ctggaaggaa gggggatcaa tatagtggaa gcgataaggc
     2881 cagtggaagc aacaacaaca ctgaatgcct gaaagccagg atggcgagaa aaaggagcga
     2941 gaaatctttg atcataaccc ttctttccga ctttggctcc ttttatcctg ctcagattaa
     3001 gggagtgatc ctctcccggc tgcagggcaa atcagaaaat atcgccggca agataacctt
     3061 cgtggatatt gcccacgaca tccccgctca ggatgtgagg gcgggggcat ttgctcttct
     3121 ctgtacggtc aggttctttc cccctggaac cattcatatt gcagtggtcg atcctggagt
     3181 ggggacagcc aggcggggcc tggtggtgga gagcggcggc cagatgttcg caggacctga
     3241 caacggcctt ctcattcccg ccgctcgttc cctgggaagg ccgacagccc atgagatcgc
     3301 ccttcctccc ggcgcttcca gcacctttca cggcagggat gtgttcgccc ccgctgctgc
     3361 cctcatcgcc gccggccagc caccggcaag cctcggtccc agagtccagc ctgtggacct
     3421 tgactttggc gaagcaaaaa agactgctcg cggccttgag gccacaatca tttatgttga
     3481 ccgtttcggc aacctcatcc tgaacttgca gaggcttccc gcccaggata tgatgctcaa
     3541 gggagtcagg ctgaagaggg cgagaaccta tgccgaaggg cggaggatcg aaccacttat
     3601 aaccctgggg agccatggct ttgcagaaat tgcgatcaac cagggaaatg ccagcgaggc
     3661 tttttgcctg aaggctcaag atcgcatcga gctggaggag atttgagttg tttggaatag
     3721 agttcgttcc cgatgaatcg gtactgaaga tcggctatat ggcgaagctg gcagaggatg
     3781 ccggatttgg aaatatctgg atcacagatc actacaataa tagggatgtc tggactaccc
     3841 tcgccgtact ctctttattg accaataaga tctcgctcgg aacgggcgtc accaacccct
     3901 acaccaggaa tgcggccatc actgcctcca gcatcgcctc cataaacgag ctctccggcg
     3961 ggcgggcgat cctgggcata ggtcccggtg acaaggccac attcgataag atggg
//
LOCUS       DAOSGP010000109         4011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_20413_length_4011_cov_18.531092, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000109 DAOSGP010000000
VERSION     DAOSGP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20413_length_4011_cov_18.531092"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PLV49_04225"
     CDS             complement(<1..419)
                     /locus_tag="PLV49_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98084.1"
                     /translation="MLINLRVWLKIPDAEAATVKNTLVRRLSYGKVLEDVKKERLISI
                     QVDSGDPEELARAFARELVNENKESHKVLIDRLEFEEGYVPVKVALHIEDGEAIAIRD
                     RLRKRLGYQNVVDVKKATVWKLYFRLLRPEAEITARE"
     gene            complement(413..1222)
                     /gene="purQ"
                     /locus_tag="PLV49_04230"
     CDS             complement(413..1222)
                     /gene="purQ"
                     /locus_tag="PLV49_04230"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOG98085.1"
                     /translation="MKIAVMQFPGTNCEFETLVAVKEVGMEGEIFRWNRPEEELSGFD
                     GFIIPGGFSYQDRVRAGAVASKEPVLKALKEEAALGKPILGICNGFQILIEAGLLPGL
                     VPGTGKVQMALAQNVMVSKGEIVRRGYYCDWVYLRHDASSRRCSGSYQIEMGALLKIP
                     IAHGEGNLVTASPGLIKKLNDQEQVVFRYCDRNGRVIDEFPVNVNGSTESIAAICNPE
                     GNVLGMMPHPERAFFAWQLPSFDPRKHRPNEPGPGRRIFESMKRYIEVESC"
     gene            complement(1219..3585)
                     /gene="purL"
                     /locus_tag="PLV49_04235"
     CDS             complement(1219..3585)
                     /gene="purL"
                     /locus_tag="PLV49_04235"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOG98086.1"
                     /translation="MHKISYQRCTQLKIIGLADPEVESSLRGAGIGMSVDEARSIAQV
                     LGRDPTLTELFCYDAMWSEHCSYKSSRATLKEFLPSDAPNVVMGPVEDSGIVAIDDKW
                     CVVISHESHNHPSQILPNEGAATGIGGIVRDVNCMGATVVATADPLRFGDPFGPKARK
                     VRWVAEGVVDGIWQYGNALGVPNIAGDVVFSESFDDNCLVNVVSLGIVRRDQIIRSRA
                     PPLAGEKGYDVILVGKPTDASGLGGVTFASEALREEDEETNRGAVQIPDPFLKNVLFK
                     ANSDLFRMVRESGIEIGFKDLGGGGFTCATSEMGAAGGFGMEIDLDEMHKAADFPPEV
                     LSIAETQERFLIIAPEELREKILNIYNEDWDLPNVYEGARASVVGKINTGDRFVVRHK
                     GETVCDVPIQHLTAGIRYQRAESERIYSESEPESREPEDYNEVLLRILNSPNIASREH
                     IYRYYDTEVMGNAVIRPGEADAGLIAPVRGEKFGVALAVDSNPFYGRISPYWGGATSV
                     AEAMRNVAAIGATPATLTDCLNYGNPEKPEAFWEFREGVKGLAESCKNLWLKDYKDER
                     IPVPIVSGNVSFYNESPQGSVDPSPVIACVGIIRDFDKAITMDLKAAGEELYLIGPRF
                     DELGGSEYYQTILGVMGANVPRVRFDLERSMIYAVIDAIDEGLIAAAHDISNGGLAAT
                     AMEMAVTRPAKFGLELDLDGVVCGDLDLRTDKLLFSESSGFLLEAKAGKEKRLEELLK
                     SYDLLPAKIGRVTAKRRIRITRKGETVVDLDLDLARNAWTRGLAEAMR"
     gene            complement(3709..>4011)
                     /locus_tag="PLV49_04240"
     CDS             complement(3709..>4011)
                     /locus_tag="PLV49_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HOG98087.1"
                     /translation="KSRVGERMAGKIDEYKKLFREATVADQMKLFKLHIAIYLVVNII
                     WLILNMMGYIKIDPALAMWYSPVGWGILVIVHYWFYIRGAENLCMLREEMVEAKIG"
BASE COUNT          815 a         1276 c          976 g          944 t
ORIGIN      
        1 atctcccggg cagtgatctc cgcctcaggc cggaggagcc tgaagtacag cttccagacg
       61 gtcgccttct tcacatccac cacattctgg tagcctaacc tctttctgag cctgtccctt
      121 atggcaattg cctcgccatc ctcgatatgc agggcaacct taaccggcac atagccctcc
      181 tcgaactcca gccggtcaat caggacctta tggctctctt tgttctcatt gaccagctcc
      241 cgggcaaaag ccctggccag ttcctcagga tccccggaat cgacctggat ggaaatgagc
      301 cgctcctttt tcacatcctc tagaaccttt ccatagctca gtcgccggac aagggtgttc
      361 ttcaccgtgg ccgcttctgc atccggtatc ttcagccaga cgcgcagatt gatcaacatg
      421 actccacctc tatgtagcgc ttcatcgact caaagatccg ccttccagga cccggctcat
      481 tgggcctgtg ctttctcgga tcgaaggagg gaagctgcca ggcaaagaac gccctctccg
      541 gatggggcat cattcccagg acgtttcctt ctggattgca gatggcagca atgctctctg
      601 tgctgccgtt aacattcaca gggaactcgt ctatcacccg gccgttgcgg tcgcagtagc
      661 ggaagaccac ctgctcctga tcattgagct tcttgatcag gccaggcgat gcagttacca
      721 ggtttccttc tccatgggct atcggtatct tcaggagagc gcccatctca atctggtacg
      781 agccgctgca ccttctggat gaggcatcgt gccgcaggta cacccagtcg caatagtagc
      841 cccggcggac gatctctcct ttggagacca tcacattctg ggccagagcc atctgtacct
      901 ttcccgtgcc agggacaaga ccaggcaaca ggccggcctc gatcaggatc tgaaagccat
      961 tgcagattcc cagaatcggc ttacctaatg ccgcctcctc tttcagagcc ttcaagaccg
     1021 gctccttgga ggcgacggct cccgccctaa ccctatcctg ataggagaat cctcccggaa
     1081 tgatgaatcc atcaaagccg gacagctcct cctctggccg gttccagcgg aagatctcgc
     1141 cctccatgcc cacctctttg actgccacca gggtttcgaa ctcgcagttc gtcccgggga
     1201 actgcatcac tgcgatcttc atctcatcgc ctccgcgaga ccacgggtcc atgcattcct
     1261 cgccagatcc aggtccaggt cgaccaccgt ctcccccttt ctggttatcc tgatccttct
     1321 tttcgccgtc accctgccga tctttgccgg caggaggtcg tagcttttca gtagctcctc
     1381 cagcctcttc tcctttcctg ctttcgcttc cagaaggaat cccgaagact ctgagaaaag
     1441 cagtttatcg gttctcagat ccaagtcgcc gcacaccacc ccatccaggt ccagctccag
     1501 gccgaactta gccggccggg ttacggccat ctccattgcc gtcgccgcca gcccgccgtt
     1561 ggatatgtca tgggctgcag caatcaagcc ctcatcaatg gcatcgatga cggcatagat
     1621 catgctcctc tccaggtcaa atcgaacccg agggacattt gctcccatca ctccgaggat
     1681 ggtctgataa tactccgagc ctcccagctc atcgaacctg gggccgataa gataaagctc
     1741 ctcgccagcc gccttcagat ccatagtgat cgctttgtcg aaatccctta tgattcccac
     1801 acaggcgatc acgggcgagg ggtccacaga accctgggga gactcattgt agaagctcac
     1861 atttcctgag actatgggta cgggaattct ctcatcctta taatccttca gccagaggtt
     1921 cttgcaggac tcggccaggc cctttacccc ttccctaaac tcccagaagg cctcgggctt
     1981 ctccggattg ccgtagttca ggcagtcggt gagagtggct ggcgtcgcgc ctatggcagc
     2041 cacatttctc atcgcctcag ccactgaggt cgctcccccc caataggggc tgatccggcc
     2101 gtaaaaggga ttggagtcca cggccagtgc cacgccgaac ttctcccccc gcacgggggc
     2161 gatgagccct gcatccgcct cgcccggcct gatcaccgca ttgcccatga cctcggtatc
     2221 gtagtatcgg tagatatgct cccgggaggc gatgttcggc gaattcagga tcctgagcag
     2281 aacctcattg tagtcctcag gctctcggga ttcgggctca gattcgctat agatcctctc
     2341 ggattcagcc ctttggtagc ggatgcccgc tgtgaggtgc tggatgggca catcgcatac
     2401 cgtctctccc ttatgccgga cgacaaacct gtcgccggta ttgatcttgc ccacaacgct
     2461 cgcccttgct ccctcgtaga cgttgggcag atcccagtcc tcattgtaga tattcaggat
     2521 cttctctctc agctcttcag gggcgatgat caagaaacgc tcctgggtct cggctatgct
     2581 cagcacctcg ggaggaaagt ctgcagcttt gtgcatctcg tccaggtcga tctccatgcc
     2641 aaagcctcca gcagccccca tctccgatgt ggcacaggtg aagcccccac caccaaggtc
     2701 tttgaacccg atctcgattc ccgactcccg caccatgcgg aatagatcgc tgttcgcctt
     2761 gaagaggacg ttcttcagga aaggatccgg gatctgcact gctccccggt tggtctcctc
     2821 gtcctcctct cgcagggctt cagaggcaaa ggtgaccccg cccaacccgc ttgcgtccgt
     2881 gggctttccc accaggataa cgtcgtagcc tttctccccc gccagcggcg gagcgcggga
     2941 tcggataatc tggtcgcgcc ggactattcc cagggatacc acgttcacca ggcagttatc
     3001 gtcgaagctc tcactaaaga ccacatcccc ggctatgttc ggcacgccca gggcattgcc
     3061 gtactgccag attccgtcca ccaccccctc tgccacccag cgaacctttc tggccttggg
     3121 gccgaaagga tcgccgaagc gcagtgggtc cgcggtggca accacagttg cccccatgca
     3181 gttcacatcg cggacgattc ccccgatacc cgttgctgcc ccctcatttg gcaggatctg
     3241 actgggatgg ttgtggctct catgggatat gaccacgcac catttgtcat ctatggctac
     3301 tattcccgag tcctcgaccg ggcccattac tacattgggg gcatctgagg gcaggaattc
     3361 ctttaaagtg gcgcgggagc tcttgtaaga gcaatgctcc gaccacatgg catcgtagca
     3421 gaatagctcg gtgagggttg gatcgcggcc cagaacctgg gcgatggaac gcgcttcatc
     3481 gacggacatg cctatgcccg ccccgcggag acttgattct acttcaggat cggcaagacc
     3541 gattatcttc aattgggtac acctctggta gctaatctta tgcatacgag gaacgttatg
     3601 aatttatcga ttcgggtgga tgttggagga attaatcaat acaaaaatag agggattaat
     3661 tgcagcgata atccccattg ctttactcga ttgagcactt ccagggtctt atcctatctt
     3721 ggcctccacc atctcttctc tgagcatgca caggttttct gctccccgga tgtagaacca
     3781 gtagtgcaca atcaccagga tgccccatcc gactggcgag taccacattg ccagggcagg
     3841 atcaattttt atatagccca tcatgttcaa tatcagccaa attatgttca ctaccagata
     3901 tatagcgatg tggagtttga agagcttcat ctggtcagca acagtggctt ctcggaagag
     3961 ctttttatac tcatcaattt ttcccgccat tctttcacct acccttgatt t
//
LOCUS       DAOSGP010000110         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_20846_length_3958_cov_15.289777, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000110 DAOSGP010000000
VERSION     DAOSGP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20846_length_3958_cov_15.289777"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="PLV49_04245"
     CDS             complement(<1..1654)
                     /locus_tag="PLV49_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG98088.1"
                     /translation="MPGKISHSYAFRGSEKPLIGKTIGDMFDEIAEKYPDNDALVSIH
                     QGKRYTYRELQKELNRTAKGFIALGLKKGDRLAIWATNIAEWIITQYATAKAGIIMVN
                     INPAYRTHELEYSLRQSEAQVLLLMDRFKSSDYVKMFYEVCPEAQTSKPGEIHSEKLP
                     FLRTAVLIRGEKQSSMYTWEEVLRMGEEIPDEVLAERQASLDFDDPINIQYTSGTTGF
                     PKGVVLTHHNILNNGYFIGECMKFTEEDRLCIPVPFYHCFGMVLSNMASVTHGTTMVL
                     PAEHFDPVSTLSAVEKERCTAVHGVPTMFVAELEHPDFSKFDLSSLRTGIMAGSPCPI
                     EFMKKVSTLMNMREVVITYGQTESSPGITMSSTDDSLDLRVSTVGKPMPHTEMKIVDP
                     KTGKIVPRGETGEICARGYMIMREYYNNPVATEQCIDEEGWLHTGDLGTLDENDYCKI
                     TGRLKDMVIRGGENIYPREIEEFLYTHPAISDVQVIGVPDKKYGEELMAWIKLKNGAS
                     ANQEEIRAFCKGRIAHFKIPRYIKFVDDFPMTVSGKIQKYKMR"
     assembly_gap    1687..1786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2491..>3958
                     /locus_tag="PLV49_04250"
     CDS             2491..>3958
                     /locus_tag="PLV49_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOG98089.1"
                     /translation="MNLRRPNANEATGTFNRSRNVVPMSGICTRCIDGCKGGCETWLA
                     SFRGREVLYPGPFGEMTAGADKNYPLDYSHLNIQGYAVGAFGLPEGVEPGPDTSIFPN
                     VDVSTEYGWSEKVSMKLPIFTGALGSTDIARKNWEHFAIGAAISGITIVCGENVCGVD
                     PELVLDGHGRVQRSPEMDRRIETYRRFHEGWGEILVQMNVEDTRLGVAEYVSSRHGLE
                     TIELKWGQGAKCIGGEIKVDRLERALELKSRGYMVQPDPGNLEVQMAYKSGAIKEFER
                     HSRLGFVSRDGFFDEVDRLRSLGFKRITLKTGAYSMAELAMAIRYGAEAKIDLLTIDG
                     APGGTGMSPWPMMNEWGIPTFYLQALAYEFATKLSRRGKKVPDIAMAGGFSTEDGVFK
                     SIAMGAPFVKAICMGRALMIPGMVGKNIGKWLEKGELPASVSKYGSRPEEIFVSYEDL
                     HAKYGNDMKDIPCGAVALYTYCQKFKTGMQQLMAGSRNF"
BASE COUNT          863 a          984 c         1070 g          941 t
ORIGIN      
        1 ctctcatctt gtacttctga atctttccgc tgaccgtcat ggggaaatcg tccacgaact
       61 tgatatacct ggggatcttg aagtgcgcga ttctgccctt gcagaaggca cggatctcct
      121 cctggttggc agatgcgcca ttcttaagct tgatccaggc catcagctcc tcgccatact
      181 tcttgtccgg aacgccgatc acctgcacat cgctgatggc ggggtgagta tagaggaact
      241 cctcgatctc tcggggatag atgttctctc ctcctcgaat caccatgtcc ttgagccggc
      301 cggttatctt gcagtaatcg ttctcgtcca gcgtccccag gtctccggta tgcagccagc
      361 cttcttcgtc tatgcactgc tccgtagcca cgggattgtt gtaatactcc cgcattatca
      421 tgtagccccg tgcacagatc tctcccgtct ccccacgggg aacaatcttt cccgtcttcg
      481 gatccacgat cttcatctct gtatggggca tgggctttcc aacagtcgag accctcaggt
      541 ccagggagtc gtcggtggat gacatggtga tccctgggga ggactcggtc tggccgtagg
      601 ttatcaccac ttcacgcatg ttcatcaggg tgctcacctt tttcatgaac tcaatgggac
      661 agggcgatcc ggccatgatt cccgtgcgca ggctgctaag atcgaacttg ctgaaatcgg
      721 ggtgctcaag ctcagccacg aacatggtgg gaactccatg caccgcggta cagcgctcct
      781 tttctaccgc ggaaagggtg gatacggggt cgaagtgctc tgccggcaag accatagtag
      841 ttccgtgagt tacagaggcc atattggaga gaaccatgcc aaagcaatga tagaatggga
      901 ccggtataca tagccggtct tcttccgtga atttcatgca ctctccgatg aagtagccgt
      961 tgttcagtat gttatggtgg gtcaggacca cgcccttggg aaatcctgtc gtgcccgatg
     1021 tatactgaat gttgatggga tcgtcgaagt caaggctcgc ctgcctctcc gccagaacct
     1081 catctggaat ctcctctccc atgcggagca cttcctccca ggtgtacatg ctggattgct
     1141 tctctcccct tatcagcaca gctgttctca ggaagggcag cttctcggag tggatctctc
     1201 ccggtttgct ggtttgggcc tcagggcata cctcatagaa catcttgacg taatccgagc
     1261 tcttgaaccg gtccatgagg aggaggacct gagcctcgga ctggcggagg gaatactcca
     1321 gctcatgggt cctgtaggcg gggttgatgt tcaccataat gattcccgct ttggcagtgg
     1381 catattgggt gatgatccat tctgctatgt tcgtcgccca tatggccagg cggtcgccct
     1441 tcttcagccc cagagctatg aatccctttg ccgtccggtt tagctccttt tgcagctcgc
     1501 gataggtgta tctctttccc tggtgaatgg agacaagtgc atcattatcc ggatatttct
     1561 cagcaatctc gtcgaacata tccccaatgg tctttccaat aagcggtttt tctgagccgc
     1621 gaaatgcata gctgtgggag attttgcctg gcatggagct tttctcaatc cctcagggaa
     1681 tataatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgt gggagatttt
     1801 gcctggcatg gagcttttct caatccctca gggaatataa tggtatctgc tctttttcgc
     1861 ttttttcatt tatgagagga tctgcaaaag cccagaaggc ctcaatccgg ccgcgcggct
     1921 gagaatctgc agggtccgaa gcagctgcct ctctgctttt cagttatgag tcccgtccgc
     1981 tagcatatgc tcttcctgcc tcattatccc agtgctttcg atttctcggc atcacacata
     2041 tatcaatttt ttattatgat gctttaagag aatttcatac gatttatgat atgaataatt
     2101 atgatcaatt ctcaaaaata actaagtaaa ggcccaagag agatttgctc aggtgaatgg
     2161 atcatgagag caagaggctg gaccacatgg gcaggttatt cgccaaaagc aagcgcaatg
     2221 atgaatacga atattttcag gctgtgcatg aagccctggg atctgatttt gccggaagag
     2281 ggaattgatg agaggctgag gagcccggag cccttctgga ggcttcatga gatgtatcta
     2341 tctgagaaag atgcagcaaa taacagcttg ctgccagtga gatccagaat agaaaggccg
     2401 gataaagtgt ttgtttgagc gccaataaat tttatttttt ttatgaaata agccaagcag
     2461 tctgcatatt tataatcaga ggtatcaagg atgaatctaa gaagaccgaa tgccaatgaa
     2521 gcaacaggaa cattcaaccg atcaagaaat gtggtgccga tgtcaggaat ctgcaccaga
     2581 tgcatagatg gctgcaaagg tggctgcgag acctggctgg cctccttcag gggaagagag
     2641 gtgctgtatc caggaccatt cggagagatg accgctggag cggacaagaa ctaccccctc
     2701 gactattccc acctcaacat ccagggttat gccgtgggag ccttcggcct tcccgagggg
     2761 gtggaaccgg gaccggacac gtccatcttt cccaatgtgg atgtctccac cgaatatggc
     2821 tggtcagaga aggtctcaat gaagcttcct atattcaccg gcgctctggg gtctactgat
     2881 atcgcccgga agaactggga gcacttcgcc atcggtgccg ctatatccgg catcaccatc
     2941 gtctgcgggg agaacgtctg cggagtggac cccgagctgg tgctggatgg ccatggtcgg
     3001 gtgcagagat ccccggagat ggaccggagg atcgagacct acaggcgctt ccatgagggc
     3061 tggggagaga tcctggtgca gatgaatgta gaggacaccc gcctgggggt ggcagagtat
     3121 gtctcttccc ggcacggcct ggagactata gagctgaagt ggggccaggg agctaagtgc
     3181 atcggcgggg agatcaaggt ggaccgcctg gaaagagccc tggaactgaa gtccaggggt
     3241 tatatggttc agcccgatcc agggaacctg gaggtgcaga tggcctacaa gtccggagcg
     3301 ataaaggagt tcgagaggca ctctcgcctg ggcttcgtct ccagggatgg ctttttcgat
     3361 gaggttgacc ggcttcgcag cctgggattc aagcgcatca ccctgaagac gggggcctac
     3421 agcatggctg agctggccat ggccattcgc tatggtgccg aggccaagat cgaccttctc
     3481 accatcgacg gcgctcctgg tggtacaggc atgagcccct ggccgatgat gaacgaatgg
     3541 ggcattccca ccttctacct gcaggcgctg gcctatgagt ttgccaccaa gctatcccgt
     3601 cgggggaaga aggtgcctga tatagctatg gccggcggct tttccactga ggatggtgtc
     3661 ttcaagtcca tcgctatggg cgcgcctttc gtaaaagcga tatgcatggg ccgggcgctg
     3721 atgattccgg gaatggtagg aaagaacatc ggcaaatggc tggagaaggg agagctgccc
     3781 gccagcgtct ccaagtacgg cagccggccg gaggagatat tcgtctccta cgaggatctt
     3841 catgccaaat atggcaatga tatgaaggat atcccctgtg gagcagtggc cctgtacacc
     3901 tattgccaga agttcaagac gggaatgcag cagcttatgg ccggatcgag aaacttcc
//
LOCUS       DAOSGP010000111         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_21181_length_3913_cov_16.256091, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000111 DAOSGP010000000
VERSION     DAOSGP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21181_length_3913_cov_16.256091"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLV49_04255"
     CDS             complement(<1..662)
                     /locus_tag="PLV49_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HOG98090.1"
                     /translation="MTPVQPLVFTLARIENLMHQVSFDPAGMKGKIITNTTRVRKEAL
                     DETLAVFYNTINSGLAVSPMIKVIEGERRIKIKTACSLTLCAVMLKHGIPVHPKGGGL
                     VEVVEREPTRFTDMLMYWATTVDPIDVLTAQGLMNITGMMRTGNGRILGNLHEAPMLA
                     RDKIEDVLEALAEAGFAGVLELGQPNMNVLGVSVERDHVGLALVGGTNLMAAAKECQI
                     DV"
     gene            767..1480
                     /locus_tag="PLV49_04260"
     CDS             767..1480
                     /locus_tag="PLV49_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase partner protein"
                     /protein_id="HOG98091.1"
                     /translation="MLRQRFVLDTTALTDSMAWESEGATDICEGMNAILDRISEGRLH
                     LGISCYIPYPSVYNEIKDFARHNSCDSRILGKVDTWLVKKTPDRYKVKVPSKIFYEYV
                     DYMRSRINKGMNISEEAVWESVSRCIALSESKGDLREMRDEIEREVVGSIIRKFREKY
                     RAALRYGILDSAPDIDVLLLAKELDAAVVSQDLGIQRWAEQLGLRFMESRSFPIMIKE
                     YMNALPELFIEEREEDRMI"
     assembly_gap    1584..1683
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1684..>3913)
                     /locus_tag="PLV49_04265"
     CDS             complement(<1684..>3913)
                     /locus_tag="PLV49_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG98092.1"
                     /translation="IVICYALVMFILRDDPEATVAFTDLALIPINGMAALALLYAAAS
                     SYQQRKKGYLTWLILSLATFSFTMGDVIWAYEETILKVDPFFSISNVFFLAYYPLFLW
                     GIFVLPSLEFPLRERFKIALDFGIVMFAGIIIFWNYIISPVVQESANYDLFTLIIYII
                     YPLGDLILLFSVLELLFRKIRGNEQTSLLLLAAGMICLIITDSIFLQLSWFDAYAAGG
                     LLDLGWPLSYALVGLAGMSQADVALKKNSFKIPEFGEFRSGGLTWPLYLPYLFAAGAF
                     ALLVWSHENPIGISFASLSWAVGMIIGLVILRQILVLNENASLYKKAQKDLQEKELAR
                     AEVIRLNEELEERVARRTKQLEDANRDLQCEVQERIQAEAAMRDSERRMADIINFLPD
                     ATFVINKEGVVIAWNRAIEMMTQVKAEDILGKGEYEYSLPFYKKRRPILIDLVTDQDH
                     NAEGCYEELKWHEDGSLAGVSFISNLNGKLTYLQGSAAVLYDSEGNIYGAIESIRDIT
                     DQKMVEEDLKNSKERAELATRSKSNFLANMSHEIRTPMNAVVGMTGLLLETDLKPEQR
                     DYLETIRTGGNTLLAIINNILDYSKIEGEKMELEILPFDLLGCIEESMDMVAAKAAEK
                     GLEIAYFQDDDVPRFIVGDQIRLRQILVNLLGNATKFTEKGEIVLSLSTTPLDDGRIE
                     LHFKVMDTGIGISQENLSRLFQFFTQVDSSTTRHYGGTGLGLAISRRLVEMMEGRIWV
                     ES"
BASE COUNT          927 a          976 c          914 g          996 t
ORIGIN      
        1 atcacatcta tctggcattc ctttgctgca gccatgagat tggtccctcc gaccagtgcc
       61 aatcccacgt gatccctctc cactgagacc cccagtacat tcatattcgg ctggcccagc
      121 tccagtaccc cggcgaatcc cgcctcggcc agagcttcaa gcacatcctc gatcttgtcc
      181 cgagcgagca taggtgcctc gtgaaggtta cctaagatcc ggccgttccc ggtgcgcatc
      241 atcccagtga tattcatcag cccttgagcg gtcaggacat caatgggatc gacggtcgta
      301 gcccaataca tgagcatatc tgtgaacctt gtcggctccc tctccaccac ctccaccaga
      361 cctcctccct tgggatgaac cggtatgcca tgcttgagca taacggcgca aagggtcagg
      421 ctgcaggcgg tcttgatttt gatcctcctc tctccttcta tgaccttgat catcggcgac
      481 accgccaggc cgctgttgat ggtattataa aagactgcca gggtctcatc cagagcctct
      541 tttcttaccc tggtggtatt ggttatgatc ttgcccttca tacccgccgg atcgaagctc
      601 acctgatgca tcaggttttc aattctggcc agggtgaaga ccagaggttg cactggggtc
      661 acatcttcca atcagcgata gggctttatt agctttttta ggggggccac tttcttttgt
      721 cctttctgga agctatctta tatcacttga tccaatacat ccagtaatgt tgcgccaaag
      781 attcgttctg gatacaactg cacttacaga ctccatggcc tgggagagcg agggcgccac
      841 cgacatctgc gaaggaatga acgccatcct ggatcgcatt tcagagggca ggcttcacct
      901 gggaatcagc tgctatatac catatccctc cgtctacaat gagatcaagg attttgccag
      961 gcataatagc tgcgattctc gcatcctggg aaaggtcgat acctggctgg tcaagaagac
     1021 tccggacaga tacaaggtca aggtgccttc aaagatcttt tatgaatatg tggattacat
     1081 gaggagccgt attaataagg gtatgaacat atctgaggaa gctgtatggg agtcggtctc
     1141 cagatgcatc gctttgtcag aaagcaaggg cgatcttaga gagatgagag atgagataga
     1201 aagggaggtc gtagggagca taataaggaa gttccgggag aagtacagag ctgcccttcg
     1261 ctatggcatt ctggatagcg ctcccgacat cgatgttttg ctgctggcca aggagctgga
     1321 tgctgcagtc gtctcccagg atttggggat tcagcgctgg gcggagcagt tgggtctgcg
     1381 cttcatggaa tccagatctt ttcccataat gattaaagag tacatgaatg cattgccgga
     1441 gctttttata gaggagagag aagaggatag aatgatctga tcaacattaa tcatcttttc
     1501 tataatctca tctaatctat tggcttcaca ctttccaagg ccgatttcag ctctcctaac
     1561 tggatcggtt tgctcagata gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnntcgctct ctacccagat ccttccctcc atcatctcca ccagcctgcg gcttatggcc
     1741 aggcccagac ctgtgccgcc gtaatgcctt gtggttgagg aatcgacctg agtgaagaat
     1801 tgaaagagac ggcttagatt ctcctgagag atgccgattc cagtatccat aactttaaaa
     1861 tgcagctcga ttcttccatc atccagaggg gttgtgctca gggataagac gatctctccc
     1921 ttctcagtga acttggtggc atttcccaga agattgacca ggatctggcg cagacggatc
     1981 tggtctccca ctataaacct gggcacatca tcgtcctgga aatacgcaat ctcaaggccc
     2041 ttttccgctg ctttcgctgc caccatatcc atcgactcct ctatgcagcc caggagatca
     2101 aatggtaaga tctccaactc catcttctct ccttcgatct tggagtaatc caggatattg
     2161 ttgataatgg cgagaagggt gtttccgccg gttcgaatgg tctccaggta gtcgcgctgt
     2221 tcaggcttca aatcggtctc caggagcagg ccggtcatgc caacgacggc attcattggc
     2281 gttctgatct catggctcat attggccagg aaattggatt tcgaccttgt ggctaattcg
     2341 gctctctctt tggagttctt gaggtcctcc tccaccatct tctggtcagt gatatcgcga
     2401 atggactcga ttgctccata gatattaccc tctgaatcgt acagaaccgc tgcactaccc
     2461 tgcagatagg tcaactttcc attgaggttt gagatgaagc taacgccagc caggctgcca
     2521 tcctcgtgcc acttgagctc ttcatagcag ccctctgcat tatgatcctg atctgtgacc
     2581 aggtcaatca gaattggcct tcgcttttta taaaagggca gagagtactc atattcgccc
     2641 tttcccagaa tatcctctgc cttgacttgg gtcatcatct ctatggctcg gttccaggca
     2701 atcaccactc cttctttatt gatgacgaag gtagcatctg ggaggaagtt tatgatatcc
     2761 gccatcctgc gctcggagtc cctcatggct gcttccgctt gtattctctc ttgcacttca
     2821 cactgcagat ctctattggc atcctccagc tgcttggttc gccgggccac cctttcttca
     2881 agctcttcgt tgaggcggat gacctctgcc ctggccagct ctttctcctg gagatccttc
     2941 tgagcttttt tataaaggct ggcattttcg ttcaggacaa gtatctgtcg cagtatgacc
     3001 aggccgatta tcatccccac tgcccaggat aagctggcga aggaaatgcc aataggattt
     3061 tcatggctcc agaccagcag agcaaatgct cccgctgcga ataaataggg aaggtagagt
     3121 ggccaggtca gccctcctga gcggaattcc ccgaattcag gtatcttgaa ggaattcttc
     3181 tttagtgcga catccgcttg agacatgcct gcaagaccga ccagtgcata ggataatggc
     3241 cagcccagat cgagaagacc tcctgctgca taggcatcaa accatgacaa ttgtaggaat
     3301 atggaatcgg ttatgatcag acagatcatt ccggcggcaa gaagaagcaa tgaggtctgt
     3361 tcattgccgc ggatcttcct gaagagaagc tccaggactg agaagagcaa gatcagatcc
     3421 cctagaggat aaatgatata gattatcaat gtgaaaaggt cgtagtttgc tgattcctga
     3481 acaactggac tgattatgta gttccagaat atgattatcc cggcaaacat tactattccg
     3541 aaatccagtg ctatcttgaa tctctctctt aaagggaatt ccaaagaggg gaggacgaag
     3601 atcccccata gaaatagagg atagtatgcc agaaagaaga cgtttgagat ggaaaaaaat
     3661 gggtcaacct tgagaatggt ctcttcatag gcccatataa catcccccat ggtgaagcta
     3721 aatgtggcta aagagaggat tagccatgtc agatatcctt tttttctctg ctgataagaa
     3781 gaagccgccg cataaaggag ggctaatgca gccatgccgt ttattgggat gagcgccagg
     3841 tctgtgaaag caactgtcgc ctctggatca tccctcagga tgaacatcac cagggcataa
     3901 cagatgacaa ttg
//
LOCUS       DAOSGP010000112         3894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_21327_length_3894_cov_19.224277, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000112 DAOSGP010000000
VERSION     DAOSGP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21327_length_3894_cov_19.224277"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..501
                     /locus_tag="PLV49_04270"
     CDS             91..501
                     /locus_tag="PLV49_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98093.1"
                     /translation="MEESEIKAIKLILAVLALGLLVAVGAAFEDYGGSHHHGGPSDGY
                     HDFGGFYDWLSPGAYYYSYSYYPTYTYYPTYYYPTYYYPTYTPVYTYTAPVVYDPVVY
                     DPWWAANVYGLGATTYYYSSSWSWSSYHGGYYFR"
     gene            complement(692..1465)
                     /locus_tag="PLV49_04275"
     CDS             complement(692..1465)
                     /locus_tag="PLV49_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG98094.1"
                     /translation="MTVKNGDFIRIDYTEFVDGQAIATTDKDLAEEKGIFSEEIQYGP
                     HLVVMGAGQLVKGFESDLVGKEIGYSGRVEMDPENAFGLHDPKNIELVPLNRFKEERP
                     VPGMRVGMDNRTGTVTRVIGRKVSIDFNHPLAGKTVVYEYTIRETVEDQTERLKALIK
                     TFGRVDLEAEIKDEIAVITVPWELNYYKEWVMIRRGLADMIIQHLGMKEVHYIEKHTG
                     EKVRAELISPPGKESGSEGPQDMESKTAEENPASGDATA"
     gene            complement(1592..2095)
                     /gene="pyrI"
                     /locus_tag="PLV49_04280"
     CDS             complement(1592..2095)
                     /gene="pyrI"
                     /locus_tag="PLV49_04280"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOG98095.1"
                     /translation="MTDKNNEKINKNKEEPGGLRVKPINNGTVIDHIAGGQALNVLKI
                     LGISGTTGATVSVVMNVESKKLGKKDIVKVEDRELREEELNRIALIAPAASINIIRNC
                     KVIEKRPVDLPDEIVGVVRCQNPNCISNTHEPIKSRMLVKAKNPVLLRCLYCEQPLAN
                     GIAEYLI"
     gene            complement(2106..3032)
                     /gene="pyrB"
                     /locus_tag="PLV49_04285"
     CDS             complement(2106..3032)
                     /gene="pyrB"
                     /locus_tag="PLV49_04285"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOG98096.1"
                     /translation="MFMKRHVLSMREFSKEEIDSVLDLAQSLEPLARGKKSRMLAGKI
                     LALLFFEPSTRTRMSFETAMKRLGGSVINLGPAEGSSISKGESLADTVRVIGGYADAL
                     VIRHPKEGSARLASQFSPVPVLNAGDGAGHHPTQTLLDLYTIRKESRLENLHVALVGD
                     LKYGRTVHSLAYALSLYGADITLVSPPTLRMPDPIKADLSSKGTIVRETADLGEVIDN
                     VDVLYMTRIQRERFPDPVEYQKVASSYRITPESLAGSQQNLIIMHPLPRVDEISPSVD
                     DSSHARYFQQSFYGVPVRMALLKMLLEGDQVD"
     gene            3167..>3894
                     /locus_tag="PLV49_04290"
     CDS             3167..>3894
                     /locus_tag="PLV49_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG98097.1"
                     /translation="MLECIAELPGEKTVVLLHVIQRKAVERVWDPTAEVAEAKEKIEE
                     QKRYLEALRIEVKTRVETIMEGSIAKTIQRVADEENATLIMVGARGRGRISGLLLGSV
                     SRDVLRYGQTDLLIMRYKVLEGDNLGRFCRSLFTKVICPTDFSDPAKDAILLIKGMKE
                     TSEIGLVYVVSSGESDEQIDAAVRVAGSKLNSIVKEIERPELKVTTHVKVGDAPHEIS
                     SLADREDASLIAMSSHGAGWLEQMV"
BASE COUNT          896 a         1050 c          983 g          965 t
ORIGIN      
        1 gcatcaagag gttcatggag gattcatata gaagctaggt gatatctaca taaaaaccac
       61 cttttaatat ttggctaggg aacaaggtgc atggaggaat cagaaataaa agcaatcaag
      121 ttgatattgg cagtgctcgc attgggactc ttagttgccg taggggcagc cttcgaggat
      181 tacggcggat ctcatcacca cggaggcccg tctgatggtt atcatgactt cggcggattc
      241 tacgactggc tcagcccagg ggcatattac tacagctata gctattatcc gacctatacc
      301 tactacccaa cctattacta tccaacctac tattacccga cctacactcc cgtctataca
      361 tatactgccc cagtggtcta cgatcctgtg gtctatgatc cctggtgggc ggcaaatgtc
      421 tacggactgg gcgctaccac atattactac tcgagcagct ggtcctggtc gtcatatcac
      481 ggcggatact acttccgcta aagcatgaat agcatattag aatatccgat gaaagatcaa
      541 agggcgctgg cattagatgg cgggcccggc tgaatatgca gcccgggcct ctccatgtga
      601 tacgatacaa ataaacggat gcaatgcctc agaagcccta tcctcggaac aaaggcaaaa
      661 aattttataa ttttatcttg gtctattacc tttaagccgt cgcatcacca gaggcaggat
      721 tctcttctgc agtctttgat tccatatcct ggggtccctc agatccgctc tcttttcctg
      781 gaggggatat gagctcggcc cggaccttct ctcccgtgtg cttctcgata tagtgcacct
      841 ctttcatgcc cagatgctgg atgatcatat ctgccagacc gcggcggatc ataacccatt
      901 ccttataata gttcagctcc cagggtacgg tgatcaccgc tatctcatcc tttatctccg
      961 cttcaagatc cacccggcca aaggtcttga taagggcttt tagcctctcg gtctgatcct
     1021 caaccgtttc ccttatggtg tactcgtaga ctacagtctt tcctgccaga ggatggttga
     1081 agtctatgct cacctttctg ccgatcaccc tggtaaccgt acccgtccgg ttatccatgc
     1141 ccacccgcat cccgggaact ggcctttctt ccttgaacct gttcagcggc actagctcta
     1201 tattcttggg atcatgcagg ccgaaggcat tctcgggatc catttccacc cttccagagt
     1261 agcctatctc ttttccgacc agatcgctct caaagccttt aaccagctgg ccggctccca
     1321 tcaccaccag atgcgggccg tactggatct cctcgctgaa aattcccttt tcctctgcca
     1381 gatccttatc ggtggtggct attgcctggc catcaacgaa ctctgtatag tcaattctga
     1441 tgaaatcgcc atttttaacc gtcatttctt actccatcct gatattactg gaaccttatt
     1501 ctcaacctgg gccgtcaatt tgcgacgatc ctatcttata ctttcgcatc ccctttggct
     1561 cgggcggtcg gttctccatc cggccgcttc actagatcaa gtattctgca atcccatttg
     1621 caaggggctg ctcgcagtaa aggcagcgta gcaataccgg attttttgct ttgaccagca
     1681 ttcgggattt tatcggctca tgagtattgg atatgcagtt gggattctgg cagcgcacca
     1741 cgcccacgat ctcatcgggc agatccaccg gtcgcttctc tatcaccttg cagttacgaa
     1801 tgatattgat ggaggccgcc ggagcgatca gtgcgatccg gttcagctcc tcctctcgca
     1861 gctcccggtc ctcgaccttg actatatcct tcttgcccag cttcttgctc tccacattca
     1921 tcaccacgga cacagtagct ccagtggtgc cggatatgcc caggatcttg aggacgttca
     1981 acgcctgacc accggcaata tgatcgataa ccgttccatt attgatgggc ttgacccgca
     2041 gccctccggg ctcttcctta tttttattga ttttttcgtt atttttatct gtcatcgcgg
     2101 aaaaatcagt caacctgatc accttctagg agcatcttca atagagccat tctcaccgga
     2161 accccataaa aggactgctg gaaatagcga gcatgagagc tgtcgtcgac agaaggtgag
     2221 atctcatcca ccctgggaag ggggtgcatg atgatcagat tctgctgtga gccggccaga
     2281 gattcagggg taatccggta gctgcttgcc accttctggt actccaccgg gtcagggaat
     2341 ctctccctct ggatgcgggt catatagagc acatccacat tgtctatcac ctcgcccagg
     2401 tcggctgtct cgcgcacaat ggtgcctttg ctcgataggt cggccttgat gggatcgggc
     2461 atcctcaggg tgggcgggga gaccagggtg atatctgctc cataaaggga gagggcatag
     2521 gccagggaat ggacggtgcg gccgtacttc agatcgccta ccagggcgac atgcaaattc
     2581 tccaggcggc tctcctttct gatggtatac aggtccagca gggtctgggt gggatggtga
     2641 cctgctccat caccggcatt gagcaccggc acgggcgaga actgggaagc caggcgggct
     2701 gacccctcct tgggatggcg gatgaccagg gcatcggcat aaccacctat cacccgaacc
     2761 gtatctgcca gcgattcacc cttggagatg gagctgccct cagccggacc caggttgatc
     2821 acgcttcctc ccagtcgctt catcgccgtc tcaaaggaca ttcgggttct ggtcgagggc
     2881 tcgaagaaga ggagagccaa aatcttgccc gcgagcattc tgctcttctt gccgcgggcc
     2941 aagggctcca gggactgggc aaggtccagg acggaatcga tctcctcttt agaaaactct
     3001 cgcatgctga gcacatgcct cttcataaac atattcgatc tcaactaatt tctgagataa
     3061 taagtcttcc acctaatacc actacattga taaactattg agcctaaatg ctattatgtt
     3121 tcagagaatt ctcattccaa ccgatttttc cagatattcg cagaacttgc ttgagtgcat
     3181 agccgagctt cccggcgaga agactgtggt gctcttgcat gtgattcaaa gaaaggccgt
     3241 ggagcgggta tgggatccga cagccgaagt cgcagaggct aaagagaaga tcgaagagca
     3301 gaagcgctac cttgaagccc tcaggatcga ggttaagacc agggtggaga cgataatgga
     3361 gggaagtata gcaaagacca tccagagggt agcagatgag gagaatgcta cgctgataat
     3421 ggttggggcg cggggaaggg gacggataag cggcctactc ctggggagcg tctcccgcga
     3481 tgttctgcgc tatgggcaaa ccgatcttct gataatgcga tacaaggttt tagagggaga
     3541 taacctgggc cggttctgca ggagcctctt taccaaggtc atttgcccca cggacttctc
     3601 agatcccgca aaggatgcga tactcctcat caaaggaatg aaagagacaa gcgagatcgg
     3661 gctggtgtat gtcgtatcca gcggcgagag cgacgagcag atagatgctg ccgtccgggt
     3721 ggctggcagc aagctcaact ccatcgtcaa ggagattgag aggcctgagc tgaaggttac
     3781 gactcatgtc aaggtgggag atgcgccgca tgagatcagc tcattggcag acagagagga
     3841 tgcatcattg attgccatga gctcccacgg agccggctgg ctggagcaga tggt
//
LOCUS       DAOSGP010000113         3885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_21412_length_3885_cov_12.737859, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000113 DAOSGP010000000
VERSION     DAOSGP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21412_length_3885_cov_12.737859"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..2021
                     /locus_tag="PLV49_04295"
     CDS             321..2021
                     /locus_tag="PLV49_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOG98098.1"
                     /translation="MKILLMGNPNVGKSVIFSRLTGIEVVSANYPGTTIEYTEGRMKL
                     GENMATLIDPPGVYSLEPTSKVEEVTGHILEQGADVVVNVIDSTNLERNLNLTLQILE
                     KGLPTVVALNLWDVATRKGIEIDTAILAEELGVDVIPTVAVSGQGIYDLVEAIGKAKT
                     PQPVHFESSDQRWARIGEIAEKSQKVLHRHPSLFDRLEDISLKPLTGIPIALLLLYLS
                     FSLVIEVGETLQLKVTDPLFIAYSNFITDLVQRHISSELLREILIGSSPELLKSFGIL
                     TTGLYIPLGIVLPFLIPFYLLLGILEDIGYLPRLSVILDAFMHRIGLHGAAILPCVLG
                     MGCSVPAVLSVRILESAKQRYLAATLMTMAIPCASQSAMIFGILAPYGLRYIFIVYAT
                     LFLSFVITGCLLHRFIGGESPEIFLEIPPYRMPNLQALFKKTFIRVREFLKEAVPYIG
                     LGMIIMNFFYLTGLMHLIGEELKPVVSGLLGLPSDAATALIIGFLRKDVGIGMFAPLD
                     MTPVQYVVAAVVLAMYMPCVATFMVMLKELGVSGTAKSVALRLVAALVVGTALNLILS
                     "
     gene            complement(2024..2302)
                     /locus_tag="PLV49_04300"
                     /pseudo
     CDS             complement(2024..2302)
                     /locus_tag="PLV49_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2458..2829)
                     /locus_tag="PLV49_04305"
     CDS             complement(2458..2829)
                     /locus_tag="PLV49_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98099.1"
                     /translation="MIIDPSESEVGTSANPCLKILNRSTCRSLTGIQCQQINLSRSER
                     LGEQLSFMKSLHDAIDFSLSILSVDLCRLPQLYDAVFPTLAPASARLGSGIRHEINDR
                     ILLCRTSSPLSTVATPPYTIM"
     gene            complement(3241..>3885)
                     /locus_tag="PLV49_04310"
     CDS             complement(3241..>3885)
                     /locus_tag="PLV49_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98100.1"
                     /translation="GAQEGREKLKSMLDLYNLNEESNILLFIDEIVSSLEMDKRDLKE
                     RPVKLTSLLRKNIKKEALYDFLYSLEYLEPKYMLKLAGKELLRLSPGERGVLLLIFYL
                     LVDNDDCPLIIDQPEDNLDNQSVFELVAPCIREARLRRQLFIVTHNPNLAVVCDAEQV
                     IAASIEKANKERVEYISGAIENPLINRKIIDVLEGTKPAFKKRESKYFLFATDA"
BASE COUNT          921 a          897 c          937 g         1130 t
ORIGIN      
        1 attcggattc aagcatatat ctatctaatt tttttaagag tattaagatt ttatagtatt
       61 tgaactttgc gtttattatt ttgatcaatg cattatctat tgatgattca tatgtcattg
      121 ggaggcgagc aatacggatt catatcatag gacaatttct cgtagtggct tctttctgat
      181 ggcaaaccag gtttttataa tggctggccg tagataggag cgagataagg tgaattcgaa
      241 tcgacccaag aatttaataa atttcatagc taattgcagt ctctagatca tatgatgcga
      301 tagagaggga gcaatagatc ttgaagatcc ttctgatggg aaaccccaat gtgggaaaga
      361 gcgttatctt ttcccggctt acaggaatcg aggtggtctc agcaaattac cccggaacca
      421 cgatcgagta tacggaagga cgaatgaagc tgggagagaa tatggccact ctaatcgatc
      481 cgcctggcgt atactcactt gagcccacat ccaaggtaga ggaggtgacc ggccatattc
      541 tggagcaagg cgcggatgtg gtggttaatg tgatcgattc caccaatctg gagagaaatc
      601 tcaacctaac ccttcaaatc ctggagaaag gccttcccac cgtagtggcc ctcaacctct
      661 gggatgtggc cacccgcaaa ggaatcgaga tcgataccgc catcctagca gaggagctgg
      721 gggtggatgt gattcccaca gtagctgtca gcggtcaggg aatctacgat ctggtggagg
      781 ctattggaaa agctaaaaca cctcagccag tgcattttga atcctctgac cagcgctggg
      841 cgcggattgg ggagatagct gagaagagcc agaaggttct gcaccggcat ccatccctct
      901 ttgaccggct ggaggacatc tcgctcaagc ccctaacggg cattcccatt gccctgctgc
      961 ttttatatct ctccttcagc ctggtaattg aggtcggcga gacgctgcag ctgaaggtta
     1021 ctgatcccct cttcatcgca tattccaatt tcataaccga tttagtgcaa agacacatca
     1081 gctcagagct cttaagggag atattgattg gcagcagtcc agagctctta aagtcctttg
     1141 gaattctgac caccggcctc tatattcctt tagggatagt cttgccattt ttaattccct
     1201 tctatctgct gctgggcata ctggaggata tcggctacct tcccaggctg agcgtcatcc
     1261 tggatgcctt catgcacagg ataggccttc atggtgctgc tatcctgccc tgtgtattgg
     1321 gaatgggctg cagcgttcct gctgttctct cggtcagaat actggagtcg gccaagcaga
     1381 ggtatctggc agcgactctg atgaccatgg ccattccctg cgcctctcag agcgccatga
     1441 tattcggcat cctcgctccc tatggcctca ggtatatctt catagtctat gccaccttat
     1501 ttctctcctt tgtgatcaca ggctgtctgc tccacaggtt cattggaggt gagtcgccgg
     1561 agatcttcct ggagatcccg ccctaccgca tgccaaatct acaagccctt ttcaagaaga
     1621 ccttcattcg ggtgcgggag ttcctgaagg aggccgtgcc ttatatcggt ctgggcatga
     1681 tcataatgaa cttcttctat ctgaccggac tgatgcatct gatcggcgag gagctgaagc
     1741 cggttgtgtc cgggcttctg ggccttccca gcgatgctgc cacggcattg attataggct
     1801 ttctccgcaa ggacgttggg atagggatgt tcgctcccct ggatatgact ccggtgcagt
     1861 atgttgttgc tgccgtggtt ttggccatgt atatgccctg tgttgccaca ttcatggtga
     1921 tgctcaagga gcttggcgtc tcgggaacgg caaaatcagt ggctttgagg cttgtcgctg
     1981 cccttgtggt tgggactgct ctaaacctca ttctcagcta gatatacaac ctcaccattg
     2041 cgtcgtgaat ctttctgtga ggcagccgat cctcatattt gagcaccgtt gccagagcaa
     2101 ttacgttgtt tccaaatttg ccttcttgcg gacagcttgg tggagtatgt gcattctcat
     2161 attgagctaa tcgggcttca agttcgctga tctttgcctt aagcggctca atttcactta
     2221 ctctggcctt aagcaactcg ttctctagct ttaattgctt gttttgttcc tgaagttgct
     2281 tgataatttc atcttggtgc atactcccaa atattggata ttatcgccat attatttaat
     2341 ctatctggtc taaataggtg ccactaaatt cccatgatac atgtgggtgc taaacacata
     2401 cgcctaaact aaacaagccc tttgatgaag gcggtttgat agcgatatca gcggtttcta
     2461 cataatggta tatggcggtg ttgctacggt cgataagggt gatgatgtcc tgcataataa
     2521 gatgcgatca tttatttcat gacgaatacc gctgcccagc cgtgctgatg ctggggcaag
     2581 tgtggggaat accgcatcat atagttgtgg cagtctgcaa agatcaactg ataagatact
     2641 caaagaaaaa tcgatcgcat catgaaggct tttcatgaag gaaagctgct cgccaagccg
     2701 ctcgctgcgg gagaggttga tctgctggca ttggatgcct gtcagactac ggcaagtgct
     2761 gcgattgagg atctttaagc agggattggc agaggtaccg acttcactct cgcttggatc
     2821 gattatcata ggagagcctg gcatctcctg ccgaggcgag cacgtgcgcg ctctcgcatg
     2881 ggcgcgcggg cacgcgtggg cctgccgcct ggggggccgg gaggccggtc gggcctgatg
     2941 tcagtgggcg ctttgcctcc ggattaggtg gctttttgag ggggctggat tttggatgca
     3001 aatgacggtt tgtggttatt attggctgca aattatagcc gtacaacttc attgcaatca
     3061 caaaacagat ctgttgacac tctttgctag cggttccaat catacttatt gggccaatcc
     3121 tgacttacat ctaatactgt caccattttc tctgtcaacg acaaacgact atctttcagt
     3181 ctataacgaa ttgaatgaaa tgcagagacg tcaagcgtcc gaagattatc cccgttcacc
     3241 ttatgcgtcc gtagcgaata ggaagtactt cgattcgcgt tttttgaaag cgggctttgt
     3301 accctctagg acgtctatga ttttcctatt gatgagaggg ttttctattg cgccagagat
     3361 gtattcaacc cgttccttat ttgctttttc tattgatgct gcaatcactt gttctgcatc
     3421 gcagactact gccagatttg gattatgagt aacaataaat agttgtcgcc taagtcgggc
     3481 ttcccgtatg cagggagcca caagttcaaa taccgattga ttatcaaggt tatcttccgg
     3541 ctgatctatg atcaacgggc aatcgtcatt atctactaat aaataaaata tgaggagtaa
     3601 tacaccacgt tctcctggag ataatcgaag taattctttt ccagccaatt tcaacatata
     3661 tttcggttcg agatactcca aagaatagag aaaatcatac aaagcctctt tcttgatatt
     3721 ttttcttagc aagctcgtca atttgactgg acgttcctta agatctctct tatccatctc
     3781 aaggctcgat actatttcgt cgatgaatag tagaatgtta gattcttcgt ttaaattata
     3841 tagatctagc atgcttttaa gtttttctct tccttcttga gcgcc
//
LOCUS       DAOSGP010000114         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_21718_length_3851_cov_17.875395, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000114 DAOSGP010000000
VERSION     DAOSGP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21718_length_3851_cov_17.875395"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PLV49_04315"
     CDS             complement(<1..1167)
                     /locus_tag="PLV49_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00297 family protein"
                     /protein_id="HOG98101.1"
                     /translation="MNRENAARLAIGLMALLLPYAWPFTVIALAAAFFVEKSTRLLAA
                     TLGFLLIIRPVLAQMSIDLPLYVIAISFVASTFASLGYLRFSSLTGSIAHLLITVALG
                     YPLAIWISAGTIPKESAMFLAVLGGLSGTFLNQASRMRDFAVPFGTAMVMWLFSFRYP
                     FPELHTIILICLFALILGVGAYWAKAADASAVISETIVGFLVIIFAGLSWFLLLLIFY
                     LMGGGFTRYGYAKKEKLGIAQSHGGARGYKNVFSNSLVPLAMAVFYGIYGNDLFVYAF
                     IGSVATANGDTLASEIGETSSSKPRMITTLKETEPGVDGGVTLLGEGASLLGALIIGI
                     LAAISGMTGLLGIVIGTAAGFLGTNFDSLLGATLQSRGTLSNNGVNLAATAFGAI"
     gene            1261..1839
                     /locus_tag="PLV49_04320"
     CDS             1261..1839
                     /locus_tag="PLV49_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018033421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOG98102.1"
                     /translation="MRISKANLPGCIAIVLSSGDLFNEGLDKLRDIIEWSRSMGLASL
                     VLYINDNEPTLCQKIADTLRSSPAQISLHTADADLLTGSGGRMRVAVSLGYGGKREVT
                     EAFRDLLSEVEEGRLIPEDIDEKAIESRLRFSQKPDLVIRAGGKQLSDFMIWQAAYSE
                     LYFTDVNWLRLRKIDFLRAIRDYQGRERRFGR"
     gene            complement(1840..2526)
                     /locus_tag="PLV49_04325"
     CDS             complement(1840..2526)
                     /locus_tag="PLV49_04325"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HOG98103.1"
                     /translation="MRKAIHDDRGGLLMIKRCPQHGFFRGEHCECGLAGQLILDEART
                     EQLGRLVAGGLRHFPLDLGLEMDSRGWVDLSKLGEVVQKRHRWASKEMVIALAQSDPK
                     QRYEISNQRIRARYGHSMDIELDHPECHLPSLYYGASEEEADRILEIGLKSASQRYVH
                     LSTTPNKAWEVAGYRTGNPKVIQVDAAPAREAGVKMMTVNDDIVISEIIPARFLCILA
                     SKDIPRLENK"
     assembly_gap    2545..2644
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2675..2905
                     /locus_tag="PLV49_04330"
     CDS             2675..2905
                     /locus_tag="PLV49_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98104.1"
                     /translation="MPGSKDVEKRICRMLDMNEEELMAELESSFILSDDCDERIKTSL
                     EEMMQEVDRLLLKIEQLEDDESEISNWESNQE"
     gene            2906..>3851
                     /gene="mptA"
                     /locus_tag="PLV49_04335"
     CDS             2906..>3851
                     /gene="mptA"
                     /locus_tag="PLV49_04335"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018033424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HOG98105.1"
                     /translation="MHLDSLPDIQACEPDFPFNLTRVGVTGVKKLIKVERGSDERPVV
                     LISNFEVFVDLPSDRKGANLSRNFEAIDEVLEDAINRPVYEIEELCGEIAKRLLARHE
                     YASRAEVKMKSEYIVRRQTPFTKVTCQEVVDIFAEAVARAGPTGCLRKTVGAEVMGMT
                     ACPCAQEIMKDQARGELIKAGLSEEVALDVLSRLPVATHNQRGRGSISVEVRDRRCVS
                     IDRIIKIIEESMSSKVYGLLKRPDEALVVSRAHSNPKFVEDCVRTMAQKTVEAFSDLP
                     DDAAVTLKQINEESIHQHNAFAERTATMGELRAELACLK"
BASE COUNT          944 a          954 c         1037 g          816 t
ORIGIN      
        1 aatggctcca aaggcagtag ctgccaggtt caccccgtta ttgctcaggg tgccccggct
       61 ctgtagggtc gctcccagca agctgtcgaa gttggtgccc aggaatccag cagctgtccc
      121 gataacgatt cctaagagtc cagtcatccc gctgatcgcg gccaaaatgc cgatgatcag
      181 tgcgccgagc agcgatgccc cttcgccaag cagcgtcacc cctccatcga cacccggctc
      241 agtctcctta agagtggtaa tcatcctggg cttggagctg ctggtctctc cgatctcgct
      301 ggccagggta tcgccattgg ccgtggccac agatccgatg aaggcgtata cgaataggtc
      361 atttccgtag atgccataga atactgccat ggccagcggt accaggctgt tgctaaagac
      421 gttcttgtag cctcgcgctc ctccatgtga ctgggctatc cctagcttct cctttttagc
      481 atatccgtaa cgagtgaacc cgccacccat cagatagaag atcaagagaa gcaaaaacca
      541 gctcaagcca gcgaagatta taaccagaaa accgactatg gtctcgctga tcacggcaga
      601 tgcgtccgcc gccttagccc agtaagcgcc cactcctaaa atgagggcga aaaggcatat
      661 gagtatgatt gtatgcagct cagggaatgg atagcggaag gagaagagcc acatcaccat
      721 agcggttccg aagggaacgg caaagtctct catcctggat gcctgattta gaaatgtgcc
      781 ggaaagacca cccaggaccg ctaggaacat agcgctctcc ttgggaatag tacccgcaga
      841 gatccagatc gcgaggggat agcccaaagc cacagtgatg agaagatgag ctatgcttcc
      901 tgttaaggaa ctaaacctca gatagcccaa tgaggcaaag gtggaggcca caaaagagat
      961 agcaataaca tacaaaggaa gatcgatgct catctgggcg agaacaggcc taatgatcag
     1021 gagaaaaccc agggttgccg caagcaaacg ggtgctcttc tccacaaaaa aagccgccgc
     1081 cagagcgatt actgtgaacg gccaggcata aggcagaagc aatgccataa gtcctatggc
     1141 cagcctggcg gcgttctcgc ggttcatgca gtacaaacca gtttcatcgc aaaaatactt
     1201 tgcctatgga aaaccagtga tgaattgatg attcaccgcc tgtatgaaag gctcctagaa
     1261 atgcgcatat ccaaggccaa tctccctggc tgcattgcca tagtcctcag ctcaggggat
     1321 ctatttaatg agggcttgga taagctgagg gatatcatcg agtggagcag atcaatgggt
     1381 cttgcttcac tcgtccttta catcaatgat aacgagccca ccctctgcca gaagatcgca
     1441 gacaccctgc ggagctctcc cgctcaaatc tctctgcaca cagcagatgc agatctgctt
     1501 acaggttccg gcgggaggat gagggttgcc gtctctttag gatatggggg caaaagagag
     1561 gtgactgagg ctttccgaga tctgctcagt gaggtggaag agggaaggct gattccggag
     1621 gatatagatg aaaaggcaat agaatcccgt cttcgcttca gccagaagcc cgatctggtg
     1681 ataagggcgg gaggaaagca gctgagcgac ttcatgatct ggcaggcggc ctactcagag
     1741 ctttatttca ccgatgtcaa ctggctaagg ctgcgcaaga tagattttct ccgtgccatc
     1801 agagactatc aggggcgaga gaggcgcttc ggccgctgat catttatttt ccagcctggg
     1861 aatatccttt gaggcgagaa tgcagagaaa tcgggcaggg attatctctg atataacgat
     1921 atcatcgttt accgtcatca tcttcacccc tgcctctctg gccggggcgg catccacctg
     1981 gattaccttg gggttcccag tgcggtagcc tgccacctcc caggccttat ttggggtggt
     2041 tgatagatgg acatacctct gggacgcgga cttgaggccg atctccagga tcctgtctgc
     2101 ctcctcctcg ctcgctccgt aatataggct gggaagatgg cattccggat ggtccagttc
     2161 tatatccata gagtgaccgt atctggccct gatcctctga tttgagatct catatctctg
     2221 cttgggatcg gattgagcaa gggcgataac catctctttg ctcgcccagc gatgtctttt
     2281 ttgaaccacc tctcccagct ttgagaggtc aacccagcct cgagagtcca tctccaggcc
     2341 aagatcaagg ggaaaatgcc gaaggccccc cgcaaccagt ctgcctagct gctccgtcct
     2401 ggcctcatcc agaataagct gaccggcaag tccgcactca caatgctctc ctctgaaaaa
     2461 tccgtgctga gggcaacgct tgatcatcag tagacctcct ctgtcatcat gaatagcctt
     2521 tcgcatagat atagctgact gctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnctcaaa tatgtccgtt ttgaataaac tattatgccc ggttcgaagg atgtcgagaa
     2701 gagaatatgc cggatgctcg atatgaatga agaggagctg atggccgagc tggaaagttc
     2761 ttttattttg tctgatgact gcgatgagag gatcaaaacc agcttggaag agatgatgca
     2821 agaagtggac aggctcctat tgaagatcga gcagctggaa gatgacgagt ctgagatcag
     2881 caactgggaa tcaaatcagg agtgaatgca tttggactct cttcccgata ttcaagcctg
     2941 cgagccagat tttcctttca atctaaccag ggtgggtgtt accggcgtca agaagctgat
     3001 taaagtcgag agggggagcg acgagaggcc cgtggtcctg atttccaatt tcgaggtgtt
     3061 cgtcgacctt ccatccgacc gcaagggtgc caacctctct cggaacttcg aggccataga
     3121 tgaggttctc gaggatgcca taaaccggcc ggtttatgag atcgaggagc tatgcggcga
     3181 gatcgccaag aggctccttg cccggcatga gtatgcatcc agggcagagg tgaagatgaa
     3241 gagcgagtac atcgttcgca gacagacgcc cttcacaaag gtcacctgcc aggaggtagt
     3301 ggacatattt gccgaggcgg tggcaagagc aggtcccaca ggatgtttaa gaaagacggt
     3361 aggtgcagag gtcatgggga tgactgcatg tccctgtgct caggagatca tgaaagatca
     3421 ggctcggggt gagctgatca aagccggcct atccgaagag gtcgctttag atgttttgag
     3481 ccgattgcct gtggccaccc ataaccagcg aggcagaggc agcatctcgg tagaggtgcg
     3541 cgaccggcgt tgcgtctcta tagacaggat catcaagatc atcgaggagt ccatgagctc
     3601 caaggtttat ggcctgctca aaaggcctga cgaggcattg gtggtgagcc gggcgcacag
     3661 caatcctaag ttcgtggagg actgcgtcag gactatggct cagaagacgg tggaggcttt
     3721 cagcgatctg ccggatgatg ctgcagtgac gttgaaacag atcaatgagg agagcatcca
     3781 tcagcataat gctttcgctg agaggactgc caccatgggc gagctgcgag cagagctggc
     3841 ctgcctgaag g
//
LOCUS       DAOSGP010000115         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_21862_length_3834_cov_18.580312, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000115 DAOSGP010000000
VERSION     DAOSGP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21862_length_3834_cov_18.580312"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLV49_04340"
     CDS             complement(<1..182)
                     /locus_tag="PLV49_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98106.1"
                     /translation="MQFAEWTYPLRKFDDIYYTIERIVYGKGELKGFTTEDIYNNLRD
                     KRFFNDVQDLNRYLLE"
     gene            307..879
                     /locus_tag="PLV49_04345"
     CDS             307..879
                     /locus_tag="PLV49_04345"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG98107.1"
                     /translation="MCPPDLILLQEKIASCELCPLSLSRNLTVPGSGPSKAEIMLVGE
                     APGREEDLKGLPFVGRAGRLLDEALDEAGLKREDVFITSVIKCRPPQNRKPKKKEIEL
                     CLPYLEDQMDAIRPRIICLMGNTAIQAVLGVVGVTVLRGRILSDRFLVTYHPAAVLRN
                     RNLMADFVSDLREAKIQSLKLSKDALLNED"
     gene            948..2237
                     /locus_tag="PLV49_04350"
     CDS             948..2237
                     /locus_tag="PLV49_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOG98108.1"
                     /translation="MKGNRVTMSGQTLSEKILSRAANKEVKAGEFVMADIDGAMIHDI
                     TGPLAVKGFYEIAGRDARVWDPTRIVLLFDHQVPADSLKAAENHIQLRKFAAEQDILN
                     YDIFCGVCHQVMPEKGHVLPGNVILGTDSHTCTYGAFGAFATGVGSTDMASVFATGKL
                     WFMVPKTLQIMIEGRLPSRVTSKDAVLRIIGDVGADGANYLACEFRGEAAERMSIPER
                     MTVSNMAIEMGAKVGIFPADGTTKSYLEGRVQDREAIEAGFINGDEDAAFSRREWNVS
                     DLEPQIAMPHNVDNVLPISQVPQTRIDQVFLGSCTNGRFEDLQLASEMMKNEPLAKGV
                     RMIVVPASRDEYMKCLRAGLVERFMEAGAMVESPCCGPCMGGSFGLLGAGERCLATSN
                     RNFVGRQGSPQAFVYLSSPATAGASAITGYITDPREI"
     gene            2293..3090
                     /gene="truA"
                     /locus_tag="PLV49_04355"
     CDS             2293..3090
                     /gene="truA"
                     /locus_tag="PLV49_04355"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG98109.1"
                     /translation="MKVALKLAYLGDNYYGFQKQPDRVTVDSQVRRALAQIGVIHGDF
                     CYAGRTDRGVSALGQVIDFWIDEDKIDLTRPRCVNGRLPRDIWTWAWSIAPVGFSARW
                     NALWREYQYLLWHPGLDRDLMEEAADMLLGEHDFRNFSSAKVGTVKRVEKLEIRERDG
                     LFIFDIRADGFLWNMVRKIVGATEKVGSGQKDMRWFKDLLRPELNHGAPTAPAEGLIL
                     MEVGYDGLDWQVDQYSRARAGRMLAGTVQKRMAGAMVALELEKAMEV"
     gene            3168..3422
                     /locus_tag="PLV49_04360"
     CDS             3168..3422
                     /locus_tag="PLV49_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98110.1"
                     /translation="MTLEETEKRLKELLGQGASQEEMIKQLSLADGCGLKLWCQEQIV
                     AGTDRKVHRWCGYVYRCQDPAENVPEPLYGKWIDDGCPEA"
     gene            complement(3493..>3834)
                     /locus_tag="PLV49_04365"
     CDS             complement(3493..>3834)
                     /locus_tag="PLV49_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOG98111.1"
                     /translation="TDVLLKEVYDASLDDIADADAIVLGGSTYNYKLIKSMDPFLKEM
                     VKLDLNGKVGVAFGSHGWSGEGVVTLIARMKSFGMRVVEPGTTAIQVPGEEDIEKCFH
                     LGRTIATGLGS"
BASE COUNT          906 a         1000 c         1119 g          809 t
ORIGIN      
        1 ttctccagca gatatcgatt cagatcctgc acatcattga aaaagcgctt atcccgcagg
       61 ttgttgtaga tatcttcggt ggtgaagccc ttcagctccc ctttgccata aactattctc
      121 tcaatagtat aatatatatc gtcaaacttc ctcaggggat atgtccactc tgcgaactgc
      181 atcagtaccc tcctcatctg gctagagggt ctgaataaat aagaaccttt cgttgcgatt
      241 gattttttcg tagggaacgg atcaatggag aagggttatc aatggcaagc ttcaatggct
      301 gccagcatgt gcccgccaga cctgatcctg ctgcaggaaa agatcgcctc ctgcgagctt
      361 tgtcctcttt ctctcagccg aaacctcact gtgcccggaa gcggcccatc caaggccgag
      421 atcatgttgg tgggagaggc ccccggtcgg gaggaggacc taaaaggact gccgttcgtc
      481 ggccgggcgg gaaggttgct ggacgaggct ctggacgagg ccggcctgaa aagagaggat
      541 gtcttcataa ccagtgtcat caaatgccgt ccgccccaga acagaaagcc gaagaaaaag
      601 gagatcgaac tctgtctgcc ctatctagag gatcagatgg atgccatccg ccccaggatc
      661 atctgcctga tggggaacac tgccattcaa gccgtcctgg gagttgtggg ggttactgtc
      721 ctgcgcggcc ggattctaag cgatcgcttt ctggtgacat atcatccggc ggccgtcctg
      781 agaaaccgaa atctgatggc ggatttcgtc tccgacctaa gagaagcaaa gattcagagc
      841 ctcaaactat caaaggacgc tctgctgaat gaggattgaa cagcgaacat ctcagccgcc
      901 gggcatgaac cgcggcatgg ccgggcggaa ggtcaagatt tattatcatg aagggcaaca
      961 gagtcaccat gagcggccaa accctgtctg agaagatatt atccagagcg gcaaacaaag
     1021 aggtaaaggc gggcgagttc gtcatggcgg atatagatgg tgccatgatt catgatataa
     1081 ccggccccct ggcagtgaag ggattctatg agattgcagg cagggatgcc cgggtctggg
     1141 acccaaccag gatcgttctt ctcttcgatc atcaggtccc ggcagacagc ctgaaggcag
     1201 cagaaaacca catccagctg cgcaagtttg ctgccgagca ggatattctc aactacgata
     1261 tcttctgcgg cgtatgccac caggtgatgc ctgagaaagg gcacgtgctt cctggaaacg
     1321 tgattttggg caccgattct cacacctgta cctatggagc ctttggggct tttgccaccg
     1381 gcgtcggctc gacggacatg gcctcagtct tcgccaccgg caagctctgg tttatggtgc
     1441 ccaagaccct ccagataatg atcgagggcc gactgccctc ccgagtaaca tcaaaggacg
     1501 ccgtcctgcg catcatcggc gatgtaggtg ccgacggggc caactatctg gcctgcgagt
     1561 tccgtggtga ggcagcggag aggatgagca ttcccgagag gatgacggtc tccaacatgg
     1621 ccatagagat gggggccaag gtgggcatct tcccggcgga cgggacgacg aagagctacc
     1681 tggagggacg agtccaggat cgagaggcaa ttgaggcggg attcatcaat ggagacgagg
     1741 atgcagcatt ctcccggagg gagtggaatg tatccgatct cgagccgcag atcgccatgc
     1801 cccataatgt agacaacgtc ctgcccatca gccaggttcc ccaaacccgg atcgatcagg
     1861 tattcttggg ctcctgcacc aacggccgat tcgaggatct gcagcttgcc agcgagatga
     1921 tgaagaatga gcccctggct aaaggggtgc ggatgatcgt ggtccccgcc agccgggatg
     1981 agtacatgaa atgcttgcga gctggcctgg tggagaggtt catggaggcg ggggcgatgg
     2041 tggagtcgcc ctgctgcggc ccctgcatgg gagggagttt cggcctcctg ggggcaggag
     2101 agcgctgtct ggccacctcc aaccgcaact tcgtgggcag gcagggaagc ccccaggcct
     2161 tcgtttacct ctcttccccc gccaccgcag gagcgagcgc cattaccggc tatatcaccg
     2221 atcccaggga gatctgagag ggatcaagga atagccagcc aatccatctt tttttttcag
     2281 gataagatca ggatgaaggt cgccctcaag ctcgcctatc tgggagataa ctattatgga
     2341 tttcaaaagc agcccgatcg ggtgactgtg gacagccagg tccgacgggc gctggcccag
     2401 atcggggtca tccacggcga cttttgctat gctggccgga cggaccgggg agtctcagct
     2461 ctgggccagg tgatcgactt ctggatagac gaggataaaa tcgatctgac caggccgcgg
     2521 tgcgtgaacg gcaggctgcc ccgggacatc tggacctggg catggtccat cgccccagtg
     2581 ggcttttctg cccgctggaa tgccctttgg cgggagtacc agtatcttct ctggcacccg
     2641 ggcctggaca gggatctaat ggaagaggca gcggatatgc tcttggggga gcacgatttt
     2701 cgaaacttct cctccgccaa ggtgggaacg gtaaagaggg tagagaagct ggagataaga
     2761 gagagagacg gccttttcat cttcgacatc cgggcagacg gctttctgtg gaatatggtg
     2821 cgaaagatcg tgggagccac cgaaaaggtg ggctcaggcc agaaggatat gcgctggttt
     2881 aaggatctcc tccggcccga attgaaccac ggcgctccta ccgccccggc agaggggctg
     2941 attctcatgg aagtgggcta cgatggcttg gactggcaag tggaccagta ttctcgggcc
     3001 cgggcgggcc ggatgctggc agggacggtg cagaagagga tggcaggggc aatggtagcg
     3061 ctggagctgg agaaggcgat ggaagtatga gttgaaacta aaaaagccat gatcctctca
     3121 tcgagaagct caaagcttaa atctgataat atcgatatct attaatcatg acactagagg
     3181 agacagagaa gagactcaaa gagctgctcg gccagggggc aagccaggaa gagatgataa
     3241 agcagctcag cctggcggat ggatgcggcc tgaagctctg gtgccaggag caaatagttg
     3301 cagggactga caggaaggtc catcgatggt gcggctatgt atatcgctgc caggatccag
     3361 cagagaatgt gccagaaccc ctctatggaa aatggattga cgatggatgt cctgaggcct
     3421 gattggatta ttgagttatt tcctaagcct cagtgcatcc gcattttttt tcttggccat
     3481 ccagaaaggg acctagcttc ccaatcccgt agcgatcgtt cttcccagat ggaagcactt
     3541 ctcaatatcc tcctcgcccg gaacctgtat ggctgtcgtc cctggctcca ctactcgcat
     3601 gccaaaggat ttcatgcggg cgatcaaggt cacaactccc tcaccgctcc acccatgcga
     3661 gccaaatgcc acacctacct tcccattcaa gtcgagcttt accatctctt ttaggaatgg
     3721 atccatcgac ttgatcagct tatagttata ggtcgagcca ccaagaacga ttgcatctgc
     3781 gtccgctata tcatctaaag atgcatcata gacttctttc agcaatacat cagt
//
LOCUS       DAOSGP010000116         3832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_21875_length_3832_cov_18.680964, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000116 DAOSGP010000000
VERSION     DAOSGP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21875_length_3832_cov_18.680964"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLV49_04370"
     CDS             <1..876
                     /locus_tag="PLV49_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98112.1"
                     /translation="SCPFCSVDAGPSSRSRVTDYQVDLDYLMEAVREIAPFKGPGVEC
                     HIDSPGEPMLYPDIVELVGRLKGIGEVSVVSMQSNGTRLDEEMIGSLEEAGLDRINLS
                     MHALDPELAAYLAGRPGFDVQGLIETAENIAKSRIDLLIAPVYIPGINDQEIPKLIDF
                     AIRSGAGKRWPPLGIQKFEHYRLGRSPRGVRAENWWHFYNRSMPQWEKEFSLPLRLKA
                     QDFEIEKRPMIPTVFKRNEKTKVEIRAPGWVEGEQLGVAKNRVVSVVDCPKKEGSVRV
                     KIVSNKHNIYVAVPA"
     gene            complement(915..1580)
                     /locus_tag="PLV49_04375"
     CDS             complement(915..1580)
                     /locus_tag="PLV49_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG98113.1"
                     /translation="MCFEEEIFTQSLLEDVERLYKDADPAHDFSHILRVYHNAKVIGL
                     AEGADMPVLLLAALLHDALEGSKHSLGSLDAKQDQDVRRREAVCQFLEKIDLPEEKKA
                     KVLYAIEVHRFSQGIEPETLEGKILQDADRLDAMGAVGIARAFMTGGSINRAFYSPQD
                     PFCKRREPDDGKWNLDHFYRKLLRLESRMHTRRGKEMASRRNEVLRRYLKDLEEEIEE
                     HSP"
     gene            1690..2874
                     /locus_tag="PLV49_04380"
     CDS             1690..2874
                     /locus_tag="PLV49_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98114.1"
                     /translation="MPDLPSSIVSAFELTVAMRHIIYRKRGTLLSVAAIALAVSISLV
                     SIFMMDGFRDMLFDVIIEDLPHVTVTPKEGDDYIYLYHSLSDRIWDIPGVVAVSASLG
                     TSATFTYKENVENVAMSGVDPEDFESIYHLEEYMIKGDLISVQSGKKVILGKKLSERL
                     KVKMGQTIYASFPDARGTSLMVSGIFDPPVGWPEDLAIVSLETARSFLNEGDVASNVD
                     IRLGDIYQADAAARSLQDYGYKADSWQKLYPEILETLAIETFENNLIMLLILIIASFG
                     VGSVMYLLVNEKTSEIGMLMAMGAKRQSIMNIFLIESGLLGLMGGAVGAVLGLALSLY
                     LGNLEFSMEAPGGQKITLPVVISLESFLVIIIAAIALSIIAGSYPAYKASRLDPTQAI
                     NA"
     gene            2882..>3832
                     /locus_tag="PLV49_04385"
     CDS             2882..>3832
                     /locus_tag="PLV49_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98115.1"
                     /translation="MKEKMMYEFSIAQRHILRNPRMALFTVAAVTLAVAIIVLFMGIM
                     SGFQEEIITTTVENNPHIYIQPKEDENYIYLYRTVSNILWAYPGVEAVSARLAGKGAA
                     KYKDEVRAISFLGVNPEDEDRMMDVRSDIISGDFQDLERRKYAAFIGTGLAEKLKIGQ
                     EDDFTLTRGNISVRIKVAGLFETGTAADDSLIYIPLMLAQDLIEMGDVVSEVDAKVAD
                     IYQVSLITTDLNRRFAYDSKSWQDMNADILNLIETQRVFAWIFYLLIFAIAGFGIANT
                     MIMIVSRRTREIGILMAMGATRRSILKVFILESLILAPPSA"
BASE COUNT          932 a          964 c         1044 g          892 t
ORIGIN      
        1 agctgcccct tctgctcggt tgatgccggt cccagctcga ggtcaagggt gacagactat
       61 caggtggacc tggactatct gatggaggcg gttcgggaga tagccccctt caagggacct
      121 ggggtagagt gccatatcga ctcccctggg gagcctatgc tctatccgga tatcgtggag
      181 ctggtaggga ggctgaaggg gatcggagag gtcagtgtcg tctccatgca gagcaatggc
      241 actcgtctgg atgaggagat gatcggatct ttagaggagg cggggctgga caggatcaac
      301 ctctccatgc acgctttaga tccggagctg gcggcgtatc tggcgggaag gccgggattt
      361 gatgtccagg ggcttataga gacagcagaa aatattgcca aaagcagaat cgacctattg
      421 atcgcgccgg tgtacattcc gggaataaac gaccaagaga ttccaaaatt gatcgatttt
      481 gccattagat caggagcggg aaagaggtgg ccgcccctgg ggattcagaa gttcgagcac
      541 taccggctgg gccgatcgcc gcggggcgtt cgtgcggaga actggtggca cttttataac
      601 cgcagcatgc cgcagtggga gaaggagttc tctctgcccc tgaggctcaa ggcgcaggat
      661 ttcgaaattg agaagcggcc catgatcccc acggtcttca agagaaatga gaagacaaaa
      721 gtggagatca gagcaccggg ctgggtggag ggtgagcagc ttggggtggc aaagaatcgg
      781 gtggtatcgg tggtggactg cccgaaaaaa gaggggagcg tgcgggtgaa gatagtatcc
      841 aataagcaca atatctatgt ggctgttcca gcttgaagat gggcattatc agctttgctg
      901 cacgcaaaat tgctttaagg cgaatgctcc tcgatctcct cctccagatc tttcagatat
      961 cttctcagca cctcatttct cctgctggcc atctcttttc ctctcctggt atgcattcgg
     1021 gattccagcc tgaggagctt tctgtaaaaa tgatccagat tccatttgcc gtcatccggc
     1081 tcacgtctct tgcagaaagg atcctgaggg ctgtagaatg cccggtttat agacccgccg
     1141 gtcataaagg ctcgggctat gccgacagcg cccatggcat ccagccggtc ggcatcctga
     1201 agtattttgc cctccagggt ttcaggctcg attccctggg agaagcggtg cacctcgatc
     1261 gcatagagca ccttcgcttt tttctcttca ggcagatcga ttttctccag aaactggcac
     1321 actgcctccc gtcgcctgac atcttgatcc tgctttgcat ctaatgagcc aagagaatgc
     1381 ttagatccct ctaaagcatc atgcagtaga gctgccagga gaagaacagg catgtccgcc
     1441 ccctcggcca ggccgatcac tttggcatta tgataaaccc ttaagatatg agaaaagtcg
     1501 tgcgcagggt ccgcatcctt atacagcctc tcgacatctt ccagcagtga ttgggtgaag
     1561 atttcttcct caaagcacat cgatctcggg ctgctttcca gccttaaata atctatcgat
     1621 ggatcttcat cgaatagaag ctcagcccag caacttgata agagaaaaca tcatagtggg
     1681 gaaaaagagg tgcccgacct gcccagcagc atcgtttccg cctttgagct gaccgtcgcc
     1741 atgaggcata tcatctatcg aaagagagga acccttctct ctgtcgcagc catagccctg
     1801 gcagtatcca tctctctcgt ctccatattc atgatggacg gctttaggga tatgctcttc
     1861 gatgtgatca tcgaggacct tcctcatgtc accgtcaccc ccaaagaagg cgacgattac
     1921 atctacctct atcacagcct ctctgatcgc atttgggaca tcccaggggt ggtggctgta
     1981 tccgccagcc tgggcaccag tgctacattc acatataagg aaaatgtgga gaatgtagcc
     2041 atgtcagggg ttgatccgga ggactttgaa agcatatacc atcttgagga atatatgatc
     2101 aaaggagacc ttatctccgt ccagagcggc aaaaaagtga tcttgggcaa gaagctctct
     2161 gaacggctga aggtcaagat gggccagacc atctatgcca gctttcccga tgccaggggc
     2221 accagcctga tggtttcagg catcttcgat ccgcccgtcg gctggccaga agacctggcc
     2281 atcgtctccc tggaaaccgc caggagcttt ctgaatgaag gggatgtggc atctaatgtt
     2341 gatataaggc ttggggatat ctaccaggca gatgccgctg ccagatctct gcaggattac
     2401 ggatacaagg cggatagctg gcagaagctc tacccggaga tcctggagac cctggctata
     2461 gagaccttcg agaacaatct gataatgcta ttgatcctga tcatcgcctc ctttggagtg
     2521 ggaagcgtca tgtacctgct ggtaaacgag aagaccagcg agatcggcat gctgatggcc
     2581 atgggcgcga aaaggcagag cataatgaat atattcctga tcgagagcgg cctcttgggg
     2641 ctgatgggcg gagcggtggg tgcagtcctg ggcctcgccc tttcccttta tctcggtaat
     2701 ctggagttct caatggaagc ccccggcggt cagaagatca ccttgccggt tgtgatcagc
     2761 ctggaaagct tcctggtgat aataatcgct gcaattgcat tgagcatcat cgcaggctcc
     2821 tatcccgctt acaaagcatc gaggcttgat cccacccagg ccataaacgc atgaagagat
     2881 attgaaggaa aaaatgatgt acgagttttc catcgcccaa agacatatcc tgagaaatcc
     2941 caggatggcc ctgttcacag ttgctgcggt gactctggcg gtggcgatca tcgttctttt
     3001 catgggaatt atgagcggct ttcaagagga gatcattacc acgacggtgg agaacaatcc
     3061 tcacatctac atccagccaa aggaggatga gaactatatc tatctttaca gaaccgtctc
     3121 taatattcta tgggcatatc ccggagtaga ggcggtctcg gcgaggctgg caggaaaagg
     3181 agccgccaaa tacaaagacg aggttcgagc aatcagcttc ctgggggtca atccagagga
     3241 cgaggaccgg atgatggatg tcagaagcga cattatctcc ggagactttc aggatttgga
     3301 gcgccggaag tatgctgcct tcattggaac cggcctggca gagaagctga agataggtca
     3361 agaggacgac ttcaccctga cgagaggaaa catatctgtc cgaataaagg ttgcaggcct
     3421 ttttgagaca ggcactgcag cagacgattc cctgatatat attcctctga tgctggctca
     3481 ggatctgata gaaatggggg acgtggtctc agaggtcgac gccaaggtgg ctgatatcta
     3541 tcaggtctca ctcatcacta ccgaccttaa ccggcgattt gcatacgatt caaaatcctg
     3601 gcaggacatg aatgccgata tccttaatct cattgagacc cagagagtct tcgcctggat
     3661 attttatctt ctgatcttcg ccatagccgg ttttggaata gccaatacca tgatcatgat
     3721 agtctcccgc cggacgcggg agataggcat tctcatggct atgggcgcca ctcgccggtc
     3781 catcctcaag gtatttatcc tggagagcct gatcctggcc ccaccttctg ct
//
LOCUS       DAOSGP010000117         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_21922_length_3826_cov_21.083002, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000117 DAOSGP010000000
VERSION     DAOSGP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21922_length_3826_cov_21.083002"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            639..2384
                     /gene="glyS"
                     /locus_tag="PLV49_04390"
     CDS             639..2384
                     /gene="glyS"
                     /locus_tag="PLV49_04390"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOG98116.1"
                     /translation="MDRNELVNELARRRGFLWPAFELYGGAAGFYDYGPLGAPLKRRI
                     EDIWRQYFVIAEGFAEIEAPTIGVEGIFQASGHLSGFSDPLTGCKECKEVYRADHLIK
                     HIIEVPDALTNEEIYRCMQENEITCPECGGELSSVYEFNLMFKTMIGPGNKMTGYMRP
                     ETAQGMFINFPRLLRYFRGALPFAAVQIGKSYRNEISPRQGVIRLREFTQAEAEIFID
                     PRDKTHPRFDEVKAMRMKFYSQEAQEKGEEEEMSFGEAVERGVVAHQTLAYYVARTYQ
                     YLLAVGIDPQKLRFRQHKSDEMAHYAADCWDAEVLLDRLGWIELVGVADRTDYDLKAH
                     TTVSKVNLSVFVNYDQPKKRKKTVVKPDFKALGPMFKSKAKAVGEALRALAPEQLAGE
                     KIQVNIDGETIDIDRSLVSFESVEEEVRGEEVVPHVIEPSFGIDRILYSILDHSYYED
                     EIDGEKRAVLRFKPRVAPIEVAVLPLMDRSELVGPAKKILEELRSRGMRTDYDTSGSI
                     GRRYRRNDEIGTPYEVTIDYETIEEGTVTIRDRDSMSQVRVARWQVVDKLQALLNGDL
                     LFQDAGDPVRSAKND"
     gene            2406..3071
                     /locus_tag="PLV49_04395"
     CDS             2406..3071
                     /locus_tag="PLV49_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98117.1"
                     /translation="MDISGKERSVGVLSLPDIVADWQKKLIGEARRKAIHLSGLSVPL
                     SLLLLGRSFTIGFVALALAISLILEWQRLKGKIRLPEVRAQEEHKVASFVYYITGCLL
                     CVVFLPKMIAVNAVLFLSLGDTISGLAGSILADADVRGRRKMWGCKSLPIMAVMFSSC
                     LLIGYLASGITQLSFPVYLAGAAAATIADGVAVIINGNSLDDNFSIPVFSGLVMTGAA
                     AFF"
     gene            3187..>3826
                     /locus_tag="PLV49_04400"
     CDS             3187..>3826
                     /locus_tag="PLV49_04400"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOG98118.1"
                     /translation="MNIILLGPPGSGKGTQAKMIADKYKVTHVSTGDILRENVRSGTP
                     LGVEAKKFMDAGKLVPDALLIDIIKDRLAKDDVKAGWMLDGFPRTMPQAEALDKILPA
                     LGQKIDVVLNIDVPDEELVKRVTGRRMCKCGTTYHVQFNPPKVEGKCDACGADLYQRA
                     DDNEETVKQRLSAYHAQTQPLIDYYNKRSLVATVLGVGDIKAIFGEVVKALDK"
BASE COUNT          921 a          909 c         1136 g          860 t
ORIGIN      
        1 agcgccaagg cctcttacaa gaacggcata ctcactctgg agctggataa aaaggagaag
       61 agaaaaggaa aagaggtgaa gatagactga ggtggatgaa agaggtccac aatgggcggg
      121 actatgtcgt cctgtcctga ttatttttct tcggcccgtt cagtgatctg ccgcatatcg
      181 cggaatcgat gataatcagc gagtaatttt acatattatg cctagacatg ggctatatga
      241 aactcatatg atctaaaatg atgggataat gctctaataa aaggccaatg tgaaaaacaa
      301 ttacttaaat gatttcgtat tatgtggatt acatgcagca gatgagtgag atttggggtc
      361 aggggattga gcttggagat gggggtcaaa agggcatact tttgggcctc agcaggatta
      421 tgcacggcaa gtatggggat gtaatcctcc tatgtactgt tatggctgcc ttcgttatgc
      481 tcctggcgtt ctctgcctcc tgctagatag agtggcaaaa atccagacaa agcaaaggga
      541 tgatatcggc ctatccaggg atatgcaaaa tgccctcttt tttaataaca aagattaaat
      601 cacccccggt attctgaaac cataccgagg attgggaaat ggacaggaat gagttggtaa
      661 atgagcttgc ccgcaggcgt ggctttctct ggcccgcttt cgagctctat ggcggagccg
      721 cgggatttta tgattacggc cctctgggag cacccctcaa gaggcgcatt gaagacatct
      781 ggcggcagta cttcgtaatt gccgaagggt ttgcagagat cgaggcccca accatcgggg
      841 tggagggcat cttccaggct tcaggccatc tgagcggctt ttccgatccc ctcaccggat
      901 gcaaggaatg caaggaggta taccgggcgg atcacctgat aaagcatatc atagaggttc
      961 ccgatgccct gacgaatgag gagatctatc gctgcatgca ggagaatgag atcacctgtc
     1021 cggagtgcgg cggcgagctc tccagtgtct atgagttcaa tctcatgttc aagaccatga
     1081 tcggcccggg aaacaagatg accggctaca tgcggccgga gactgcccag gggatgttca
     1141 tcaacttccc ccggctcctg cgctacttcc ggggggctct tccattcgcg gcagtgcaga
     1201 tcggcaagtc ctatcgaaat gagatctccc cccgccaggg agtgatcagg ttgagggagt
     1261 tcacccaggc cgaggcggag atcttcatcg atcccagaga caagacccat cccagattcg
     1321 atgaggtcaa ggctatgcgg atgaagttct actcccagga ggcgcaggag aagggggagg
     1381 aggaggagat gagctttggc gaagcggtgg agaggggagt tgtcgctcac cagaccctgg
     1441 cctattatgt ggccaggacc tatcagtacc ttctggcggt gggcatagac ccgcaaaagc
     1501 tccgcttccg acagcacaag tccgatgaga tggcccatta tgctgctgac tgctgggatg
     1561 cggaggtcct tttggaccgt ctgggctgga tagagctggt gggagtggca gacaggaccg
     1621 actatgacct caaagcccat accaccgtca gcaaggtgaa cctctctgtc tttgtgaact
     1681 atgaccagcc gaagaagagg aagaagaccg tggtcaagcc tgatttcaag gctttggggc
     1741 cgatgttcaa gtcgaaggcc aaagcggttg gagaagccct tcgcgctctg gcaccagagc
     1801 aattggccgg tgagaagatt caggtaaata ttgatgggga gacgatagat atcgaccgaa
     1861 gtctggtttc ctttgagagc gtggaggagg aggtgcgggg agaggaggtg gtgcctcatg
     1921 tgatagagcc ctcctttggc atcgatcgga tactctattc catcctggat cactcctact
     1981 atgaggatga gatcgatgga gagaaaagag cggttctccg gttcaagcct cgagttgcgc
     2041 caatcgaggt ggcggtcctg cccttaatgg atagaagcga gctggtcgga ccggcaaaga
     2101 agatcctgga ggaattgcgt tcccggggca tgcgaactga ctatgacacc tcgggatcca
     2161 tcggaaggcg ctaccggcgc aatgatgaga tcgggacccc ctatgaggtc actattgact
     2221 atgagaccat tgaagagggc actgtcacca tcagggatag ggatagcatg agtcaggtca
     2281 gggttgctcg ctggcaggtt gtcgacaagt tgcaggccct tctcaatggg gaccttctgt
     2341 tccaggatgc tggcgatccg gtccgttcag caaagaatga ttgattggaa aaaggcgagt
     2401 gggaaatgga tatctcggga aaggagcgtt cggtgggagt cctatccctg cctgatatag
     2461 tcgccgactg gcagaaaaag ctaatagggg aggcgagaag aaaggccatt cacctctctg
     2521 gcctctctgt ccctctctct cttctgctct tgggcaggag ctttaccatc ggctttgtgg
     2581 cccttgctct ggctatctct ctgattctag agtggcagag gctgaaggga aaaatccgtc
     2641 ttccagaggt gcgagcccag gaggagcaca aggtggccag cttcgtctat tacatcactg
     2701 gttgcctcct gtgtgtcgtt tttcttccta agatgatagc ggtaaacgcc gtgctgtttc
     2761 tctctttggg cgataccatc agcggtctcg caggatcgat tctggccgac gccgatgtgc
     2821 gaggaagaag gaagatgtgg ggctgcaagt cccttccgat catggccgtc atgttctcca
     2881 gctgtcttct gatcggatat ctggcctcgg gcatcaccca actctccttt ccggtctatc
     2941 tggcaggagc ggctgcggca acgattgccg acggtgtggc agtgatcata aacggcaata
     3001 gccttgacga taacttctcc ataccggtgt tctccggcct ggtgatgacc ggggcggcag
     3061 cattttttta gagctcctcc tcatttgggg tcaatggaaa aggtttttta gtctggggat
     3121 tgtactcttt cagcgctcaa aagcagcaaa tagctgctag gttatgggaa taggaggatt
     3181 ggataaatga atattatact gctcggcccg ccgggatccg gcaagggcac acaggctaag
     3241 atgattgccg acaagtacaa ggtcactcac gtctctaccg gtgacattct gagggagaac
     3301 gtccgcagcg gcacccccct gggagttgag gccaagaagt tcatggatgc aggaaagctg
     3361 gttcctgatg ccctgctcat cgatatcatc aaggaccgcc tggccaagga tgatgttaag
     3421 gccggatgga tgctggatgg attccccaga accatgcctc aggctgaggc actggacaag
     3481 atcctgcccg ctctgggcca gaagatcgat gtggtcttga atatcgatgt tccagatgag
     3541 gagctggtca agagggttac aggaaggagg atgtgcaagt gcggcaccac ctatcacgtt
     3601 cagttcaacc cgccaaaggt tgaaggaaag tgcgatgcct gcggagcaga cctctaccag
     3661 agggcggatg acaacgaaga gacggtcaag cagaggctgt ctgcctatca cgctcagacc
     3721 cagcccttga tcgactacta caacaagcgc agcctggtgg ccacagttct tggcgtgggc
     3781 gacatcaagg ccatcttcgg cgaggtcgtc aaggctctgg acaaga
//
LOCUS       DAOSGP010000118         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_22004_length_3817_cov_19.953216, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000118 DAOSGP010000000
VERSION     DAOSGP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22004_length_3817_cov_19.953216"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLV49_04405"
     CDS             <1..745
                     /locus_tag="PLV49_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98119.1"
                     /translation="SEKSIDFHHGGRVPVKFDVRDYQADSSGVHLLIYSPDLAIVDIY
                     HMLIKDGKALYVARESPVSISDGIRNIDFDWKQLLVRGEQYSGRVKIVEKETGQTRAF
                     MSSFLAREDARITDTYEDETGASATILGKSMTPFHGFLRFLLSQDERSLATLEKKTPV
                     LLKGDDETIEVSWNKTLNPGIYQLKIQLLGTEGDLIDFTERVIEAKARTNLTAVKTEA
                     PKDSSSSMVLGLLGLVAFAGIIYVIRRRR"
     gene            747..1913
                     /locus_tag="PLV49_04410"
     CDS             747..1913
                     /locus_tag="PLV49_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98120.1"
                     /translation="MLAFSSFEIFVAMRHITYRKRQAALAIAAVGMAVAVSLLIIAVQ
                     NGFSDYMLDIVFKSLPHIIIKPTDGENYLNLYQNVIDGVWSLEGVVGVSPVLAATATF
                     THEDKVVNAAMLGVLPSQADKISKMSESMVQGDLNSIIGGKRILLGRSLAEKLDMKLG
                     DTVHADFPDARDLNLTVSGIFDTGYASVDEKTSYVSLETAQQFLNEGNVVTQIDIKLN
                     DIDQAQAVAGQISSDRYEVQVWQESNPEIVESLAYEKASNMITLLLIMVIATFGIASI
                     MNMLVLEKTREIGMLMAAGADSSHIRKIFLLESGLLGLMGAFLGCAMSLVLAIRLRSL
                     QIEMPTGGMVDLPVILSYQDMVTLSLIALILSLAAGIYPAHIASKLDPVESLRG"
     gene            1916..3076
                     /locus_tag="PLV49_04415"
     CDS             1916..3076
                     /locus_tag="PLV49_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98121.1"
                     /translation="MFELIIAWRHITASPRMVLFPVLAVALAVMVIAAMMGLMESYRE
                     EIISSTVENSPYLTVEPKEGEDYIHLYKTLSSVIWGYPGVEAVSPRLLGMAAAKYREQ
                     VKGVSIIGIDPLLEDPLLGVQSDLVWGDFNDLAFKKHSAIVGAKLAEDLKLKPGDGFY
                     LIRKGVSLKLTPAGYIRTGTGADESLVYLPLETAQRLMGEGDVVSEIGVRTSDLYEGP
                     AIASGLSRDSFYKAQSWQDKSRNLLNLLDTQRFFTYLFYLLIFVISGFGVVNTMIMTI
                     SRRTREIGILMAIGANRGSIIKIFMAESLILGPPSAALGCLLAYVAAKLIEAFPIEIP
                     AEVYAASRMSILLSPEIFAYAVAFAIMVDLAAGLYPAYKASRMDPVEAIASA"
     gene            3073..3816
                     /locus_tag="PLV49_04420"
     CDS             3073..3816
                     /locus_tag="PLV49_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG98122.1"
                     /translation="MNLVLIYQENDIMNIIVAKKLRKVFGNGVELLALDDVNLTILEG
                     EFLSITGPSGSGKSTLLNMLGLLDKPSGGEIWLKGREVSQSSSLERSQLRNKELGFIF
                     QYHHLLPEFTALENVMIPQLIAGADKKAARHRSEELLREMGLVERLNHKPSELSGGQS
                     QRVAVARALANRPSIVIGDEPTGNLDTKSSEMVYELLRKLNREIKQTFILVTHDMNMA
                     QKTDRILRLVDGRIVSEMRRENGGFVECL"
BASE COUNT          915 a          959 c         1078 g          865 t
ORIGIN      
        1 atcagagaag agcattgact ttcaccatgg tgggcgggtt ccggtgaagt tcgatgtaag
       61 ggactatcaa gcagacagca gcggggttca tcttctgatc tattctccgg acctggcgat
      121 agtggatatc tatcacatgc tcattaaaga tggaaaagcc ctatatgtcg ctcgcgaaag
      181 ccccgtttcc atatcagacg gcattcgcaa tatcgatttt gactggaagc agcttcttgt
      241 gaggggcgag caatactcgg gtcgggttaa gatcgtggaa aaggagaccg gtcagacccg
      301 agccttcatg agctcctttc tggcccggga ggatgcgagg attaccgaca cttatgagga
      361 cgagacaggt gccagcgcca cgattctggg gaaatccatg actccctttc acggtttcct
      421 gaggttcctc ctctcgcagg atgaaaggtc cctcgctact ctggagaaga agacccccgt
      481 tcttctcaaa ggagatgacg agacgattga agtgtcctgg aacaagaccc tgaaccccgg
      541 gatataccag ctgaaaatcc agctcttggg tacagaagga gatctgatag actttactga
      601 gagggtgatc gaggcaaagg ccaggactaa tctgaccgcc gtcaagacag aagcgcccaa
      661 agactcatcc tctagcatgg tcttaggcct gcttggcctg gtcgcttttg cagggatcat
      721 ttatgtaatt cgcaggaggc gttgatatgc tggccttttc ctcattcgag atcttcgtgg
      781 ccatgagaca cataacttac cgcaagcgcc aagcagctct ggccatagca gctgttggaa
      841 tggcggtggc cgtatccctc ctgatcatag cagttcaaaa cggcttctcc gattacatgc
      901 tggatatagt attcaagagc ctccctcata taatcatcaa gcccacagac ggagagaact
      961 acctcaacct ctatcagaat gtcatagatg gcgtctggag cctggagggg gtagtagggg
     1021 tctcaccggt actggcggcc accgccacct tcacccacga agacaaggtc gtgaatgcag
     1081 caatgcttgg cgttctgcct tcacaggctg ataagatcag caaaatgagt gagagcatgg
     1141 tgcagggaga tctgaactcc atcattggag gaaaaaggat cctcttgggc agatctttgg
     1201 cagagaagct cgacatgaag ctgggagata cggtgcacgc tgactttccc gatgccaggg
     1261 acctcaacct cacagtatcg gggatcttcg atactggtta tgcctccgtc gatgagaaga
     1321 catcctatgt atccctggag actgctcagc agtttttgaa cgaagggaat gtggtgactc
     1381 aaatcgatat caaactgaat gacatcgacc aggcccaagc cgtggctggg cagatctctt
     1441 cagataggta cgaggtccag gtttggcagg agtccaatcc tgaaatcgtg gaaagcctgg
     1501 cctatgagaa ggcctctaac atgatcaccc tgcttttgat catggtcatc gccaccttcg
     1561 gaatagccag catcatgaat atgctggtcc ttgaaaagac acgggagata gggatgctga
     1621 tggcagcagg ggctgacagc tctcatatca ggaagatctt cctcctggag agcggccttc
     1681 tcggcctcat gggggccttc cttggctgcg ccatgagcct cgttttagcc atcaggcttc
     1741 gaagccttca gatcgagatg cccaccggcg gcatggtcga tctgcccgta atcctctcct
     1801 atcaggatat ggtcacgctc tcattaatag cgctgattct gagccttgcg gccgggatct
     1861 atcctgcaca tatagcctcg aagcttgacc cggtagagtc cctgagggga tagagatgtt
     1921 tgagcttatc attgcctgga ggcatatcac agccagcccc aggatggtgc tcttccccgt
     1981 cctcgctgtt gccctggctg tgatggttat agcagctatg atggggctca tggagagtta
     2041 cagggaggag atcatcagca gcacagtgga gaacagcccc tacctgacgg ttgagccgaa
     2101 ggagggtgaa gactatatcc acctctacaa gacgctctca agcgtgatct ggggctatcc
     2161 cggggttgag gcggtctcgc ccaggctttt ggggatggct gcggccaagt acagagaaca
     2221 ggtgaaagga gtctccatca taggcatcga cccgctgctt gaagatcctc tcctgggtgt
     2281 gcaaagcgac ctggtctggg gcgatttcaa tgatctggct ttcaagaagc attcggccat
     2341 cgttggagcc aagctggcag aagatctgaa gctgaagcct ggggatggtt tttacctgat
     2401 tcgcaaggga gtgtctttaa agctaacacc cgcaggctac atccggactg gaaccggagc
     2461 tgatgagtcg ctagtgtacc ttcccctgga gactgctcag aggctgatgg gagagggaga
     2521 tgtggtctct gagattgggg ttagaacatc agacctctat gagggccctg ccattgcttc
     2581 cgggttgagc cgggacagtt tctataaggc ccagagctgg caggataaga gccggaacct
     2641 cctgaatctc ctggatacac agaggttttt cacttacctc ttttacctgc tcatattcgt
     2701 catctccgga tttggcgtgg tcaacaccat gattatgacc atctcccgca ggaccagaga
     2761 aattggcatc ctcatggcca tcggtgccaa cagaggatct ataatcaaga tctttatggc
     2821 cgagagcctg atcctcggtc ctccatcagc cgcactgggc tgccttttgg cttatgtcgc
     2881 agccaagctg atcgaggcat ttccgattga gatccccgcg gaggtctatg ccgcttccag
     2941 gatgagcatc ctcttaagcc ccgagatctt cgcctatgcc gtagcttttg ccattatggt
     3001 ggatctggca gcgggactgt atcctgccta taaggcatct cgaatggatc cggtcgaggc
     3061 catagcctcg gcttgaattt agtattaatt tatcaggaga atgatatcat gaacataatc
     3121 gtcgctaaga agttgagaaa ggtcttcgga aacggcgtgg agttgctggc tctggacgat
     3181 gtcaacctaa cgatcctgga gggggagttc ctctccataa ccggtcccag cggcagcggc
     3241 aagtccactc tgctgaacat gctcgggctg ctggataagc catcgggagg cgagatatgg
     3301 ctgaagggca gggaagtgtc gcaatccagc tccctggaga gatcccagct tcgaaataag
     3361 gaactgggat tcatcttcca gtatcaccat cttttgccgg agttcactgc gctggagaac
     3421 gtcatgattc ctcagctcat tgccggggca gacaaaaaag cagctcgcca taggagcgag
     3481 gagctattgc gggagatggg actcgtggaa cggctgaacc acaaaccaag cgagctgtca
     3541 ggaggccaga gccagagagt ggctgtagcc cgggccctgg ctaacaggcc atccatcgtg
     3601 atcggcgacg agcccactgg gaacctggac accaagagct cggagatggt ctatgagctg
     3661 ctgagaaagc tgaaccggga gataaaacag accttcatcc tggtgacaca tgatatgaac
     3721 atggctcaga aaaccgatcg aatcctccgg ctggttgacg ggaggatagt gagcgagatg
     3781 cgcagagaga acggcggatt tgtagagtgc ctgtgag
//
LOCUS       DAOSGP010000119         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_22136_length_3803_cov_18.174760, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000119 DAOSGP010000000
VERSION     DAOSGP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22136_length_3803_cov_18.174760"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..770)
                     /locus_tag="PLV49_04425"
     CDS             complement(30..770)
                     /locus_tag="PLV49_04425"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOG98123.1"
                     /translation="MSEPAASLKGYLIRGLPIIKEGDDIPTLIQSLFEIEDGDVICLA
                     STIVAKSEGRMRHLDDYQPSSYAIEAARDLGKDPRFVQAVFEESSDILIERPFLLTVT
                     HFGHIGVNAGIDQSNVGDEMILLLPIDPCSSAQRLRQALGRDCAVIITDTCGRPFRNG
                     VAGVAVGWSGIAALRDWRGELDIHGRALSITQEAIADEIAAAANLLMGEAGDLTPAVV
                     FRGLKYPRSGDDLFRAKEKDIIRSRLNR"
     gene            complement(806..1039)
                     /locus_tag="PLV49_04430"
     CDS             complement(806..1039)
                     /locus_tag="PLV49_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98124.1"
                     /translation="MEEKISLTFTEEHKYQLEFFPPLFWREFAEGYGGLPWIEISDAR
                     TAIVAANYSYLLDLLVQARLYRLSRLPSGSRPQ"
     gene            complement(1040..1669)
                     /locus_tag="PLV49_04435"
     CDS             complement(1040..1669)
                     /locus_tag="PLV49_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HOG98125.1"
                     /translation="MDDYLAGLDRAMKKMPANRESKERFVIPSAKVFYEGKTTVLENF
                     GAIADTLNRDPEHLMKYLLQEMGTAGKIEGQRGIFQGKFGETAIARQIESYFEEYVVC
                     TECRLPDTHLIKSDRVLTLKCDACGAHRPVRKRKATTAVQKDVIEEGETYELRIESVG
                     NKGDGIAKVDKYLIFVPSAIKGEIVRAKIKKISGTLAFAEVVERKGKVS"
     gene            complement(1675..2190)
                     /locus_tag="PLV49_04440"
     CDS             complement(1675..2190)
                     /locus_tag="PLV49_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOG98126.1"
                     /translation="MTRKPASMYRRHERPYTRRKYMGGVPGSKVVHYDMGNLQEDYPL
                     KLTLVVMEPCQIRHTALEAARVAANRYLLKQMGRTNFHLKLRTYPHHVIRENKQATGA
                     GADRVSSGMRMAFGKAVGTAARVEAGQKLFTLGIPPQYLNDAKEALTRAGHKLPTPIQ
                     IIVESGQELIR"
     assembly_gap    2253..2352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2353..3518)
                     /gene="gatD"
                     /locus_tag="PLV49_04445"
     CDS             complement(<2353..3518)
                     /gene="gatD"
                     /locus_tag="PLV49_04445"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOG98127.1"
                     /translation="MDLGDRVLVERNGISYEGVLMPSRRADHVVIKLKNGYNIGLKAR
                     ESDIKLIEKGKEIKSQQLDRPLRDKPGLPQLSILSTGGTIASKVDYRTGAVTSQFSAG
                     EIISAIPELEEIANYRARVIYQILSENMRSDYWTALARSVAEEIERGAEGVIITHGTD
                     TMMYTAAALSFMLKTPVPVVLVGSQRSSDRPSSDASMNAVCAATVATSDIAEVSVVMH
                     GTTNDDYCTIHRGTRVRKMHTSRRDAFQTINQHPLGRVDYLSRKVETYIPFCHRGEVE
                     MELNERLEPRCSLVKYTPGSSPEILHYYIDKGYRGVVLEGTGLGHVASDWIEGIGRAK
                     EENIPVVVTSQCLRGRICDRVYDTGRDMLDAGAIEGGDMLPEVALVKLMWVLAN"
BASE COUNT          785 a         1051 c          954 g          913 t
ORIGIN      
        1 tataatgcaa tctccgaact gtcaaaatct catctattca atcgggaccg gattatgtct
       61 ttctcctttg ccctgaagag atcatctcca gagcggggat acttcagccc tcggaagacc
      121 actgcagggg tcagatcccc agcctcaccc atcaatagat tggcagcagc agcgatctca
      181 tccgcaatcg cctcctgggt gatactgagt gctcttccat gtatgtcgag ctcaccgcgc
      241 cagtctcgca gagcagcgat gcccgaccaa ccaacagcca caccggcaac gccattgcgg
      301 aatgggcggc cacaggtatc ggtgattatg acggcacaat ccctgccgag agcctgacgc
      361 agcctctggg cgctgctgca gggatctatc ggcaagagca gtatcatttc atcgcccaca
      421 ttggactgat caatcccggc attaacgccg atatggccaa agtgggtcac agtcaagaga
      481 aatggtcgct cgatcaatat atcgctggat tcttcaaaga ccgcctgcac aaatctgggg
      541 tcctttccca gatcgcgggc ggcctctatg gcgtagctgc ttggttggta gtcatccaga
      601 tgcctcatcc tgccttctga tttggccacg atggtgctgg caaggcagat gacatccccg
      661 tcctcgatct caaagagaga ctggataagc gttgggatat cgtctccttc ttttataatc
      721 ggcaggccac ggatcagata accctttaaa gatgctgctg gctcagacat aacctatcga
      781 tccgaaacct gtagactgca attctctact gtggtcgcga gccagatggc aatctgctca
      841 ggcggtacaa cctggcctga accaggagat cgagcagata agagtagtta gcagcgacta
      901 ttgcagttcg cgcgtcgctg atctctatcc agggaagacc accataacct tctgcaaact
      961 ccctccagaa caaaggaggg aaaaactcca gttgatactt atgctcttct gtaaaggtga
     1021 ggctgatctt ctcctccatt caggacacct tgcccttcct ctccacaacc tcggcaaagg
     1081 ccagggttcc gctgatcttc ttgatcttag ctcggacgat ctcgcctttt attgcgctgg
     1141 gcacgaagat gagatacttg tcgaccttgg ctatgccgtc gcctttattg cccacagact
     1201 cgatgcgcag ctcataggtc tctccctcct cgatgacgtc cttttgaacc gctgtcgttg
     1261 cctttctctt gcgcaccggc cgatgtgcgc cgcaggcatc gcacttgagg gtgaggacgc
     1321 ggtcgctctt gatgagatga gtatctggaa gcctgcactc tgtgcagacg acatactcct
     1381 cgaagtagga ttctatctgg cgggcgatgg cggtctcgcc aaactttccc tgaaagatcc
     1441 ccctctgtcc ctcgatcttt ccggctgtac ccatctcctg gagaagatac ttcataaggt
     1501 gttccggatc acgattcaga gtatcagcta tagccccaaa gttctcaagg acggtggtct
     1561 tgccctcata gaataccttc gcagaaggta tcacaaacct ctccttggac tctctgttgg
     1621 cgggcatctt cttcatggcc ctatccaggc ccgctaaata gtcatccatc cagattacct
     1681 tatcagctct tgaccggatt caactatgat ctgaatggga gtgggcagct tatgtccggc
     1741 tctggtcaat gcttctttag catcgttgag gtactgaggg ggtatcccaa gggtgaagag
     1801 cttctggccg gcttcaactc tggccgcggt ccctaccgct ttgccgaagg ccattctcat
     1861 tccgctggat acacggtccg cacctgcgcc ggtggcctgc ttgttctcgc gaatgacatg
     1921 atggggatat gttctgagct tgaggtggaa gttggtcctg cccatttgct tcaggaggta
     1981 acggttggct gctactcttg ccgcttccag ggcggtgtgc ctgatctggc agggctccat
     2041 gacaaccagt gtcagcttta ggggatagtc ctcctgcaga ttccccatat catagtgaac
     2101 taccttgctt cccggaacgc cccccatgta ctttctgcgc gtgtagggac gttcatgcct
     2161 tctatacatg cttgctggct tgcgtgtcaa aactaattcc tccaataatc ctgaggttgg
     2221 tgcactattc accttgctta taaggttttg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnttattggc cagcacccac atcagcttga ccagggccac ctcaggcagc
     2401 atatcgcccc cttcaattgc accggcatca agcatatctc gaccggtatc gtatacccgg
     2461 tcacatatcc tgccccgcag gcactgtgag gtcacgacca ccggtatatt ctcctccttt
     2521 gccctgccaa ttccctctat ccagtcggag gcgacgtgac ctaagccagt cccctccagg
     2581 actactcccc ggtaaccttt atcgatatag taatgcagga tctccggaga ggatcccggg
     2641 gtgtacttga ccagactgca cctgggctca agcctctcat tcagctccat ctccacctcg
     2701 ccccggtggc aaaagggaat ataggtctct acctttctgc tgaggtaatc taccctgccc
     2761 agagggtgct ggttgatggt ctggaaggca tcccgacgag aggtatgcat cttgcgaacc
     2821 cgtgtgcctc gatggatggt gcagtagtcg tcattggttg tgccgtgcat gaccacgctg
     2881 acctcggcga tatcgctggt ggcaacggta gctgcacaga cggcgttcat ggaggcatcg
     2941 ctgctgggac gatcggagct cctctgggag ccgaccagca ccacaggaac cggggtcttt
     3001 agcatgaagg agagtgcggc agcagtgtac atcatggtgt cagtgccgtg ggttatgatg
     3061 actccttctg cgcccctctc gatctcctct gccaccgacc gcgcgagggc cgtccagtag
     3121 tccgaacgca tattctcgct caggatctgg tagatcaccc gagctcgata gttggcgatc
     3181 tcctccagct cagggatggc ggagatgatc tcgccggcgg agaactggct ggtgactgct
     3241 cccgtccggt agtcaacctt gctggctatt gtcccaccgg ttgagagaat ggatagctgg
     3301 ggcaggccag gcttatccct gaggggtctg tccaattgct gggatttgat ctccttgcct
     3361 ttttctatga gcttgatgtc gctctctctt gccttaaggc cgatgttgta gccgttcttg
     3421 agctttatga ccacgtgatc cgcacggcgg gatggcatga gaaccccctc ataggagatg
     3481 ccgttcctct ccaccaagac tctgtctccc agatccattt cgtcctcacc agaatgcaaa
     3541 tttgcttctg tcactattgc tcgtatctaa aaacgttccc cttgggagat ggaggcagcg
     3601 gcatggtatc agatagtgat ctggcgctcg aaaaggctta tcatataggg gagaaggata
     3661 gcataaggag aaaaccatgc acagatggga tcctaagacc tatgagaaga gctcgtctgc
     3721 ccagcagaca tgggcgcagg agcttttatc caagatctca atcaggggag atgaaaggat
     3781 acttgatatc ggatgcggag acg
//
LOCUS       DAOSGP010000120         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_22295_length_3784_cov_19.522660, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000120 DAOSGP010000000
VERSION     DAOSGP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22295_length_3784_cov_19.522660"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLV49_04450"
     CDS             complement(<1..212)
                     /locus_tag="PLV49_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOG98128.1"
                     /translation="MGKVFAGSIGDKEIVNIDGTVLGDLENIIYEVMTGKLVDLVVKP
                     NSGLDRSKYREDGKFVLIPFSSVVAV"
     gene            complement(308..2521)
                     /locus_tag="PLV49_04455"
     CDS             complement(308..2521)
                     /locus_tag="PLV49_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HOG98129.1"
                     /translation="MREFKDIILKVAKAYPNDSARGIARLDPNALLTLRLSPGDIIEI
                     EGKRLTAAKVWRADRQDWSQDYIRIDGFIRQNAGVGIGDKVKIRKAKFAEAQRIVLAP
                     PSGSHMHYGDEAADMIRRQTLKRPVVAGDILPIMSSGSHPFVGRMEPVPLVVTETHPD
                     NVVVVCERTEIVLLEKPAKSVRSVKATGITYENVGGLRSEVQRVREMIELPMKHPEIF
                     QKLGIEPPKGVLLYGPPGTGKTLIAKAVANESGANFISIAGPEIMSKYYGESEQRLRE
                     IFEEAQKSAPSIIFIDEIDSIAPKRGEVTGEVERRVVAQLLAMMDGLKERGQVVVIGA
                     TNREEAIDPALRRPGRFDREIEVGVPDREGRIEILQIHMHSMPVADDVNLEGLADRMH
                     GFVGADVNALCKEAAMKALRRYLPDLTSEDEIPQEIIDQMQVMGADFEEALKEIEPSA
                     MREVLVEVPRVNWNDMGGLGALKQELIESIEWPIKQPEKFQKMGIRPPKGILLYGPPG
                     TGKTMIAQAVANETNANFISIRGPQMLSKWVGESEKAIREIFRKARQVSPAIIFFDEL
                     DSIAPMRGMDEGGRVMERVVNQLLAELDGLEALKDVVVIAATNRPDILDPALLRSGRF
                     DRMLLVGPPDRQGRHEILKIHASRTPKGEDVSLEELAELTDGYVGSDLDNLCREAAML
                     ALREGLDRVEMRHYREALKKVRPSVEEQMLSYYERIGERFKGGIKVEPASLAGYR"
     assembly_gap    2586..2685
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2726..>3784
                     /locus_tag="PLV49_04460"
     CDS             2726..>3784
                     /locus_tag="PLV49_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG98130.1"
                     /translation="MEEYEELIAAARCEIEVDLLLLGGSVANLISGEVRRSDVAVHKG
                     RIVGFECSSARQIIPLEDQILAPGFIDGHVHIESSMVTVPEYARAVVPQGTTTVVADP
                     HEIANVWGEEGVRYILQSSRKVPLNVFIMLPSCVPATDMETAGAALGPGAIAGLLEEE
                     GVIGLAEVMNCPGVIFRDPQIMRKIQLAGGRLKDGHAPGLSGPDLSAYICAGIRSDHE
                     CTTLAEAEEKLFSGMFIMLREGSAARNLTDLLPLVTLKNSRQFLFVSDDRHPADILRE
                     GHIDNMVRKAINEGLDPIVALQISSLNAARYFGLADLGAIAPGYRADIAVLDGLDEPR
                     VTHVFKDGRLVARDGDFLA"
BASE COUNT          706 a         1155 c          964 g          859 t
ORIGIN      
        1 ttgaccgcca ccaccgagct gaatggtatc agcacaaact tgccgtcctc tcggtacttg
       61 ctgcgatcaa ggcccgagtt tggctttacc accaggtcca ccagcttgcc ggtcatgacc
      121 tcgtagataa tattctccag atctcccagg actgtgccat cgatgtttac gatctcttta
      181 tctccaatgc ttcccgcgaa cacctttccc atgcccctaa aaccctcctc tccaatttaa
      241 ttgtcaagac ttgagcatca attcaagcat catgcgcaga ttcaagccat cattctattc
      301 cgccaggcta gcggtagccg gcgagcgaag ccggctcaac cttgattccg cccttgaacc
      361 tctcgcctat gcgctcatag tagctgagca tttgctcctc cacgctcggc ctcaccttct
      421 tcaaagcctc ccggtagtgt ctcatctcca cccggtccag cccttcccgc agagcaagca
      481 tggccgcctc ccggcagagg ttgtccagat ctgagcccac atagccatcg gtcaattccg
      541 ccagctcctc caggctcaca tcctctcctt tgggcgtcct ggaggcgtgg atcttgagga
      601 tctcatgcct cccctgccgg tcaggcggcc ccaccaggag cattcggtcg aaccttcccg
      661 atctaagcag cgcgggatcg aggatatccg gccggttggt ggcggcgatc accaccacat
      721 ccttcaaggc ctccaggccg tcaagctctg ccagaagctg gtttaccacc ctctccatca
      781 ctctgccccc ttcgtccatt cccctcatgg gggcaatgga gtccagctca tcgaagaata
      841 tgattgccgg cgagacctgc cttgccttgc ggaatatctc tctgatcgcc ttctccgact
      901 cgcccaccca tttggagagc atctgcggcc cccggatgct tatgaagttg gcgttagtct
      961 cattggccac cgcctgagcg atcatggtct ttcctgtgcc cggcgggccg tagagcagaa
     1021 ttccctttgg gggccttatg cccatcttct ggaacttctc cggctgcttg atgggccact
     1081 ctatggactc tatcagttcc tgctttagag cccccaatcc tcccatgtcg ttccagttga
     1141 cccggggcac ctctaccaga acctctctca tggccgaggg ctcgatctcc ttgagagcct
     1201 cctcgaagtc cgcgcccatg acctgcatct gatcgatgat ctcctggggt atctcgtcct
     1261 cagaggtgag atcgggcaga tagcggcgca gggctttcat ggccgcctct ttgcacaggg
     1321 cgttaacatc tgccccgacg aaaccatgca tacggtccgc caacccctcc aggttcacat
     1381 catccgccac gggcatgctg tgcatatgta tctgcaggat ctcgattctg ccctccctgt
     1441 ccggaacccc gacctcgatc tccctgtcga accggcccgg ccggcgcaga gcgggatcaa
     1501 tggcctcttc ccggttggtg gctccgatca ccaccacctg gcctctctct ttcaggccat
     1561 ccatcatggc caggagctgg gccaccactc ttctctccac ctcccctgtg acctcacccc
     1621 gcttaggggc aatggagtcg atctcatcga tgaatatgat ggatggagcg gacttttgcg
     1681 cctcctcgaa gatctccctc agcctctgct cgctctctcc atagtatttg gacatgatct
     1741 ccggcccggc aatggagatg aagttagctc cactctcatt ggccactgct ttggcgatga
     1801 gggtctttcc cgtgcccggc gggccataga gcagaacacc tttgggaggc tcaatgccaa
     1861 gcttctggaa gatctcagga tgcttcatgg gcagctcgat catctctctt acccgttgca
     1921 cctctgagcg caggccgcct acattctcat aggtgattcc cgtggctttg accgatcgga
     1981 cgctcttggc cggcttttcc aggagaacga tctccgtcct ctcgcagacc accaccacat
     2041 tatcgggatg agtctctgtt accactaaag gcactggttc catccggcct acaaagggat
     2101 ggctgccgga gctcatgatg ggaagtatat ctcccgccac caccggccgc ttgagcgtct
     2161 ggcggcggat catatcagct gcctcatccc catagtgcat atgcgatccg gaaggaggag
     2221 cgaggacgat cctctgcgcc tcggcgaact tggccttgcg gatcttcacc ttgtccccga
     2281 ttccaacgcc ggcgttctgg cggatgaagc cgtctattct tatataatcc tgagaccagt
     2341 cctgccggtc ggcgcgccag accttggctg cggtaagcct cttcccctca atctcgatga
     2401 tgtcacccgg actgagcctc agggttagaa gggcattggg gtccaggcgg gcgatgcccc
     2461 tggcggagtc gttgggatag gctttggcca ctttcagaat tatatccttg aattcacgca
     2521 cagtttagtt tatctatgct tctcgattta aatttatctg gtgtcgatga ggattttttt
     2581 attcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnattta aataggaccg
     2701 actgtaattg attatcatgg gccagatgga agaatatgag gagttgatag ctgccgccag
     2761 gtgtgagatc gaggtggatc ttcttctcct tgggggatcc gtagccaatc tcatatcggg
     2821 cgaggttcgc agatcggacg ttgccgtaca caagggcagg atcgtgggct ttgaatgcag
     2881 cagcgctcgc cagatcatac ccctggagga ccagatcctg gcgccaggat ttatcgacgg
     2941 ccatgtccat atcgaaagca gcatggtcac agttccagag tacgcgcgag cggtggttcc
     3001 tcaaggtacc accaccgtgg tcgccgatcc gcatgagatc gccaacgtct ggggcgagga
     3061 gggggttcgc tatatcctgc agtcatcgag aaaggttccc ctgaacgtct tcatcatgct
     3121 cccctcctgt gtcccagcca ccgacatgga aacagccgga gccgcactgg gcccgggtgc
     3181 cattgcaggt ctcttggaag aggagggggt cataggcctg gccgaggtaa tgaattgtcc
     3241 tggcgtcatc ttccgggacc cgcagatcat gagaaaaatc cagctggcag ggggacggct
     3301 caaggacggc catgcccccg gcctctccgg cccggacctg tctgcctata tttgcgccgg
     3361 aatccgctcc gatcacgagt gcaccacact ggccgaggca gaagagaagc tgttttccgg
     3421 gatgttcatc atgctgcggg agggaagcgc agcccggaat ctcacggatc ttctgccgct
     3481 ggtcacgctg aaaaattctc gccagttttt attcgtctca gacgaccgtc acccggcaga
     3541 tatcctccgg gaagggcata tcgacaacat ggttagaaag gcgataaatg agggtctgga
     3601 ccccatagtt gccctgcaga tctcctctct caatgctgcc cgctacttcg gcctggccga
     3661 cctgggggca atagcacccg gctatcgggc agacatcgcc gtcctggatg ggttggatga
     3721 gccgcgggtt actcatgtct tcaaggacgg caggctggtg gccagggatg gagatttcct
     3781 cgcc
//
LOCUS       DAOSGP010000121         3764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_22492_length_3764_cov_18.366945, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000121 DAOSGP010000000
VERSION     DAOSGP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22492_length_3764_cov_18.366945"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..>1121
                     /locus_tag="PLV49_04465"
     CDS             55..>1121
                     /locus_tag="PLV49_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOG98131.1"
                     /translation="MSDELLETYIRQYLEAQRIPQATIAWQGGEPTLMGLDFFRRSIQ
                     IEKKYSRPNMIIQNTMQTNGTLLDDEWCEFFRENSFLIGLSLDGPRDFHNAYRVDKTG
                     KPTFDRVMKAARLLRHHKVDYNILTTVHAANGDHPLEVYRFLRDEVKANFIQFIPIVE
                     RDNATGFQEGEVVTNRSVKAEQYGRFLISVFNEWVCRDVGRIFVQIFDVALAAWSGMP
                     SGMCAFSETCGTAMALEHNGDMYSCDHFVEPKYLLGSIWKKDMAGLASSTKQQKFGRD
                     KLDALPEYCRRCEVRFVCNGECPKNRFIKTPDGKAGLNYLCAGYKEFFRHIDGPMRFM
                     AHELRLGRAPAGIMKYTKDGK"
     assembly_gap    1122..1221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1222..3509)
                     /locus_tag="PLV49_04470"
     CDS             complement(<1222..3509)
                     /locus_tag="PLV49_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOG98132.1"
                     /translation="MSNEDVISPEQETVLVIKSEKSESTSASSQGTTEAGEKCVPQIG
                     LTYADSTPCPIETVKAPKGSPNVVFLILDDIGYGGLGCFGGPVETPNIDRLAQGGLRY
                     TNFHTTGICSPTRACMLTGRNLHSVGVGSLMEFASGYPGYTTYLSKEAATMPEMLVGQ
                     GYDTYCVGKWHLAPSDSINAAGPYDQWPMSRGFERFYGFLESHTSQWYPDLVSGSTRI
                     TPPATPEEGYHLSKDLVNQSIQYISDGKSLQPDKPFFLYLAFGAGHWPHHVPQAYIDK
                     YKGRFDMGWDAMRNQTLAKEKELGVVPENTDLAPRDQWVKAWDNLTEDEKKVYARFAE
                     VHAAYIDYTDEQIGRFLDYLEDSGQLNNTMIVVISDNGASPEGDANGWTNMVMWANSV
                     SEGGDSSGFQNDFLPIGNITNISTMLSKIDEIGGPLSYPTYPLGWAMADNTPHKLYKW
                     TSHEGGTHDPMIIYWPDGIKEEGGIRSQFCHAIDVVPTVLDVLEFDAPEVYNGVTQKP
                     IEGISLAYTFANSTEPTHKKVQYFEMMGTRAIWYDGWKAVAFHHLDSGPDFQNDTWEL
                     YNLTDDVSEVHNLADQYPAKLEEMKDRWWAEASKYNVLPLDDRAGARYSALQARGIST
                     FTYLPGVEKIMEPAIPDTRNSSYTLTAYVNTSGNDSEGVLFSIGGRFAGLSLYVKDKH
                     LIFDYNLFNMKHYTVSSINEVPEGEATLGFEFEKTGRWKGEGTLFINGNEEGSVDMSF
                     TVPARYSFEEGLEVGQDPQTPVT"
     gene            3604..3759
                     /locus_tag="PLV49_04475"
     CDS             3604..3759
                     /locus_tag="PLV49_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98133.1"
                     /translation="MASAAIVWLINWLSEIAKASDASAELLLSAEKLSDAAIANKKIK
                     KAIVTVA"
BASE COUNT          846 a          940 c          965 g          913 t
ORIGIN      
        1 aagtactgct tctttctctc aaagaagagc ctttatcctg gcagcagctt caggatgtcc
       61 gatgaactac tggagacata tatccgccag tacttagaag cgcagagaat tccacaggct
      121 accatcgcct ggcagggcgg cgagcccacg ctgatgggct tggatttctt ccggcgttcg
      181 atccagattg agaagaagta cagccgtccg aatatgatta tccagaacac catgcagacc
      241 aatggcactc tgcttgacga tgaatggtgc gaattcttcc gcgaaaacag ctttctcata
      301 ggtctatccc tggacgggcc cagagatttt cacaacgcct atcgagtgga caagactgga
      361 aagcctacct ttgacagggt gatgaaggcg gcccgactcc tgcggcatca taaggtggac
      421 tacaacatcc ttaccacagt tcatgccgcc aacggcgatc atccactgga ggtttaccgt
      481 tttctcagag atgaagtgaa agctaatttc atccagttta tacccatagt ggagagggac
      541 aacgccaccg gctttcagga gggagaggtc gtcaccaacc gctctgtaaa ggcggagcag
      601 tatggccgtt tcttgatctc agtcttcaat gaatgggtgt gccgagatgt agggaggatt
      661 tttgttcaga tattcgatgt ggctttagcc gcctggtctg gaatgccttc cggaatgtgc
      721 gcattttcag agacttgcgg cacggcaatg gccttggagc ataacggaga catgtactct
      781 tgcgatcact ttgttgaacc gaaatacctt ctgggcagca tctggaagaa ggacatggcc
      841 ggattggcca gctcgacaaa gcagcagaag ttcggtcggg ataagctcga tgcgctgccg
      901 gaatattgcc gccgctgcga ggtgagattc gtctgcaatg gcgaatgccc caaaaaccgc
      961 tttataaaaa cgcctgacgg aaaagctggt ctaaactatc tatgcgccgg ctacaaggag
     1021 ttcttcaggc acattgacgg gccgatgcgg ttcatggctc atgaactccg cctgggccgg
     1081 gctccagctg gcattatgaa atacaccaag gacggcaaga tnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn ngtcacaggc gtctgcggat cttgtccaac ctccaaaccc
     1261 tcttcgaagg aatagcgtgc gggcacagtg aaagacatat ccacgcttcc ttcctcattg
     1321 ccattgatga agagggtgcc ttcgcccttc caccttccag tcttctcgaa ctcaaagccc
     1381 agggtggcct ctccttcagg tacctcattt atcgaggaga ctgtgtaatg cttcatattg
     1441 aagagattgt agtcgaagat gaggtgcttg tccttgacat agagcgacaa tcctgcgaac
     1501 ctgcctccta tggagaagag aacgccttcg ctatcgtttc cggaggtgtt cacatatgca
     1561 gtcaaagtgt aggagctgtt gcgcgtgtcg gggatagctg gctccatgat cttttctact
     1621 ccgggcaggt aggtgaaggt cgatattcct cttgcctgga gagcggagta ccttgcgcct
     1681 gcgcggtcat ccaggggcag cacgttatac ttactggcct cggcccacca gcgatccttc
     1741 atctcttcaa gcttggcagg atactggtct gccaggttgt gtacctcaga gacatcgtcg
     1801 gtgagattgt acagctccca ggtatcgttt tggaagtcag ggcctgagtc taggtggtgg
     1861 aacgcaaccg ccttccagcc atcgtaccat atggcgcggg ttcccatcat ctcgaagtac
     1921 tggaccttct tatgagtagg ctcggtggag ttagcgaagg tataggccag gctaattcct
     1981 tcgataggct tctgggtgac gccgttgtaa acctcaggag catcgaattc caggacatca
     2041 agaacagttg gcacaacatc gatagcgtga cagaactgcg accggatacc gccctcttcc
     2101 ttgatgccat caggccagta tattatcatt ggatcgtgag ttccgccttc atggctggtc
     2161 cacttgtaga gcttgtgggg ggtgttgtcg gccatagccc atcccagagg ataggtcggg
     2221 tatgacagag gtccgccaat ctcatcgatc ttggagagca tagtgctgat attggttata
     2281 ttgccaatgg gcaggaagtc gttttggaat ccggacgagt ctcctccttc ggacaccgag
     2341 tttgcccaca tgaccatgtt agtccaaccg ttggcgtcac cctctggaga tgcgccgtta
     2401 tcagatatca ccactatcat ggtgttattg agctggccgc tgtcctccag gtaatccaag
     2461 aaccttccaa tctgctcatc agtgtaatcg atgtatgcgg cgtgaacctc agcaaacctg
     2521 gcataaacct tcttctcgtc ttctgttagg ttgtcccacg cttttaccca ctggtcacgt
     2581 ggcgccagat cggtgttctc aggtacgact cccaattcct tctctttagc cagagtctgg
     2641 tttctcatgg cgtcccaacc catatcgaat cggcccttat acttatcgat gtaggcctgg
     2701 ggaacatgat gcggccaatg gccagcaccg aaggccaagt aaaggaagaa gggcttgtcc
     2761 ggttggaggg attttccgtc gcttatgtac tgtatggact ggttcaccag gtccttggag
     2821 agatggtagc cctcctctgg ggtggctggc ggcgttatgc gggtactgcc ggaaaccagg
     2881 tcagggtacc actggctggt gtgggactca aggaatccat agaacctctc aaatcccctg
     2941 ctcatcggcc actggtcgta aggtcctgct gcgttgatag aatcggaagg cgcaaggtgc
     3001 cacttgccca cgcaatatgt atcgtaccct tgtccaacca gcatttcagg catggtagct
     3061 gcctctttgg acagataagt cgtatatccg gggtagcccg atgcaaactc cattaatgaa
     3121 ccgactccta cagaatgaag atttctgccc gtgagcatac aggcacgggt tggagagcag
     3181 ataccagtgg tgtggaagtt ggtgtacctg agacctcctt gagccaatcg atctatattt
     3241 ggagtctcga caggtccgcc gaaacatcca agaccaccat atccaatgtc atctaaaatc
     3301 aagaacacta cgttgggcga tcccttcgga gcctttacgg tctcaatggg acatggtgtt
     3361 gagtctgcat aagtcaggcc aatctgaggt acgcatttct cacctgcctc ggttgtgccc
     3421 tgagaagaag cggacgtaga ttcgctcttt tcagacttga tgaccagtac ggtctcctgc
     3481 tcgggcgaga ttacgtcttc attggacaca tctgccaggg tcgaactctc agataagacc
     3541 tttcttctat tcgtaaccag ttccaggcca gagtcttcca gcgatccgtt gctggagcga
     3601 cccttggcat cagccgctat tgtctggctg atcaattggc tttctgaaat cgctaaagca
     3661 tctgatgctt cagcagaatt gctgctatct gcagaaaagc tatcggatgc tgctattgcg
     3721 aataaaaaaa tcaaaaaagc cattgtcact gtggcttgat ttgg
//
LOCUS       DAOSGP010000122         3731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_22761_length_3731_cov_16.533460, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000122 DAOSGP010000000
VERSION     DAOSGP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22761_length_3731_cov_16.533460"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLV49_04480"
     CDS             complement(<1..542)
                     /locus_tag="PLV49_04480"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOG98134.1"
                     /translation="MRPILFVDNHDSFVWNLVDYVSVFYDNTIVRSNEISMAEVESIN
                     PRGIVISPGPGHPAKARDIGSCLEIILGHPKTPILGVCLGHQAMNLAYGGTISHTRPL
                     HGKTSQIEHDGRGIFRGVENPLQGGRYHSLAIERLSPEVEACARSAEGVVMGIRHRNR
                     PHCGLQFHPESVLTPSGKRI"
     gene            complement(539..1993)
                     /gene="trpE"
                     /locus_tag="PLV49_04485"
     CDS             complement(539..1993)
                     /gene="trpE"
                     /locus_tag="PLV49_04485"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOG98135.1"
                     /translation="MPSDPVAPVFIDVSNKVSVDAPLSLYMALRDRRYPYLLESVEKS
                     GQKARFSFVGADPSAMISIKNRFLEMEFFSGGMGPMTERLSVCVDVERKGSVLQGPVK
                     DGCDLFDVLRAAIPSRRGAPNSFSRQVFTGGGIGFLAYDLVKERLGKGSTSQTPDAQF
                     GLAESTFIFDHLDRKVYFSIAPILPGPKVNLIETIGGLDPDYNPEEDPNISGRVLSAG
                     APEEYKDAVSRAKEHIIDGDIFQVVLARSTRIECPDPVKLYLKLRSINPSPYTYLFEF
                     GDLAIVGASPETLFNTYNRMLKVNPIAGTCPRGRTQSEDDRYARIMLNDEKERAEHVM
                     LVDLGRNDVRSVCRSGTVQVEDFMSVLQYSHVQHIETTVIGELREECDQFDAARSVFP
                     AGTLSGAPKLRAMEIIDDLERAPRGIYGGGIGYFSIDGSSDFAIAIRSVLVKDGVATV
                     QAGAGIVADSDPEKEYQETERKMAAMKRALGVAA"
     gene            complement(1975..2616)
                     /locus_tag="PLV49_04490"
     CDS             complement(1975..2616)
                     /locus_tag="PLV49_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOG98136.1"
                     /translation="MTRVKICGIMNSDDLALALQAGADAVGFVVEIEDSRHCISAEEA
                     ADLIHKVPVYSKSVAVIHPCDLDDALRLADITRADVLQLHSSLPPSDVADLKSRTGSK
                     LVVAVAADNGGLQAERYETVADAILLDSMVEGRLGGTGRVHDWDKSASIVRSLQVPVI
                     LAGGLDPENVLQAIERVRPYAVDVSSGTETAGRKDPFKVRAFVQEVRRCPATQ"
     gene            complement(2613..3638)
                     /gene="trpD"
                     /locus_tag="PLV49_04495"
     CDS             complement(2613..3638)
                     /gene="trpD"
                     /locus_tag="PLV49_04495"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOG98137.1"
                     /translation="MSYLQKIMEGQDLSQEESEALMDEIFNKASDAMIGAVLVALRRK
                     GESVSEIAGFARKMRESAIRIEPSVSGTLVDTCGTGGDGSNTINVSTAAAIVTAACGV
                     PVAKHGNYAISSKCGSANVLDALGVRISSDPAEVQSLIQDVGIGFMLAPAFHPAMKRV
                     GPVRRELGIRTVFNILGPLTNPAGAKAQVMGVYDPSLCEKLAYVLQILGAKRAMVVNG
                     EGMDEITNTGQTRVSELKDGSVKSYTLRPEDLGYPQAESADIAGGMPDENAKRLVEVL
                     RGETSAARDIIVINSGAAIYVSGLVSTLKKGASMAEDAIDSGEALKTLQRMVKASGEP
                     EKLERFL"
BASE COUNT          744 a         1175 c          976 g          836 t
ORIGIN      
        1 atgatcctct ttcccgaggg ggtaagcacc gattccggat ggaactgcag gccgcagtga
       61 ggccgattcc tgtggcggat gcccataacc actccctcag cgctccgggc acaggcctcc
      121 acctccgggc tgagccgctc tatggccagg gagtggtagc gaccgccctg caggggattc
      181 tccacccccc ggaatatgcc ccggccatca tgctcgatct gggaggtctt gccgtgaaga
      241 ggcctggtat gagatatagt ccccccatag gccaggttca tggcctggtg gcccagacac
      301 acccccagga tgggcgtttt tggatggccg agaattatct ccaggcagct gcctatatcc
      361 ctggccttgg ccggatgacc tggaccgggg gagatcacaa tccccctggg gttaatgctc
      421 tccacctccg ccatagagat ctcattggag cggacgatgg tgttatcata aaagacggag
      481 acataatcca ccaggttcca gacgaacgag tcatgattgt ccacgaagag aatcggcctc
      541 atgccgccac tcccagggct cttttcatag ccgccatctt cctttccgtc tcctggtact
      601 ccttctctgg gtccgagtcg gccactatcc ccgcccccgc ctgcactgtg gccacgccgt
      661 ccttgaccag aacgcttctt atggctatgg cgaagtccga gctcccgtca atggagaagt
      721 agcctatacc tcccccataa attcctcggg gagccctctc caggtcatcg atgatctcca
      781 tcgcccgcag cttgggagca ccggataggg tgcccgcggg aaataccgac cgcgcggcat
      841 cgaactggtc gcactcctcc cttagctctc caatcaccgt ggtctctatg tgctggacat
      901 gagagtactg cagaacagac atgaaatctt ctacctgaac cgtccccgac cggcagacgg
      961 aacgcacgtc attcctgccc aggtccacca gcatcacatg ctccgccctc tccttctcat
     1021 cattgagcat aatccgagca taccggtcgt cctcagactg ggttctcccc cggggacagg
     1081 tcccggcgat gggattgacc ttcaacatgc ggttgtaggt attgaagagg gtctcaggcg
     1141 atgctcccac aatggccaga tcaccgaact cgaagagata ggtgtaaggg ctgggattga
     1201 tgctcctcag cttcaaatag agcttgactg ggtccgggca ctcaattctg gtcgatcggg
     1261 ccaagacgac ctgaaatatg tccccgtcga tgatatgctc cttggcccgg gatacggcat
     1321 ccttatactc ctcaggcgcc ccggccgaga gcaccctgcc tgatatattc ggatcctctt
     1381 ccggattata gtcgggatct aatccgccga tggtctcaat cagattgacc ttggggccgg
     1441 gcagaatggg ggcgatggag aagtagacct tgcggtccag gtgatcgaag atgaaggtgc
     1501 tctccgccag gccgaactgg gcatcagggg tctgggaggt cgagcctttc cccagcctct
     1561 ctttcaccag gtcgtaagcc agaaagccta tccctcctcc agtgaacacc tgcctggaga
     1621 agctgttggg cgctccacgg cgggagggta tggccgccct caggacatcg aagagatcac
     1681 agccatcctt taccgggccc tggagaactg agccctttct ttccacatcc acgcaaaccg
     1741 agagcctctc cgtcatcggc cccattccgc cgctgaaaaa ctccatttca aggaagcggt
     1801 tcttgatcga gatcatggcc gatggatcgg ctcccacaaa agagaacctg gccttctgcc
     1861 cggacttttc caccgactcc aggagatagg ggtacctgcg atcgcgcaga gccatataca
     1921 gggacagggg agcgtctacg ctgaccttat tgctcacatc tatgaatacc ggagctactg
     1981 ggtcgctggg catcttctca cctcctgtac aaatgctcgg accttgaagg gatcctttct
     2041 gcccgcggtc tctgtgccgc tggagacatc cactgcatag ggccttaccc gctctatggc
     2101 ctggaggaca ttttccggat caagcccccc ggccaggatc accggcacct ggagggatct
     2161 gactatactg gcgctcttgt cccagtcgtg cacccttccc gttccgccca gccttccttc
     2221 gaccatgctg tccaggagta ttgcatccgc taccgtctca tatctctctg cctgcaggcc
     2281 gccgttatct gcggccacag caaccaccag cttggagcca gtcctgctct ttagatcagc
     2341 tacatctgaa ggcggcagcg atgagtgcag ctggagaaca tctgcccgcg ttatatcggc
     2401 cagtcgcagc gcatcatcta aatcacaggg atggatcacc gccacgctct tgctgtaaac
     2461 cggcacctta tggatcagat ccgctgcttc ttccgccgaa atgcagtgcc tggagtcttc
     2521 gatctccacc acaaatccca ctgcatccgc tcctgcctga agggcaaggg ctagatcgtc
     2581 gctattcata attccgcaga tcttgaccct ggtcataaga agcgctccag cttctcaggc
     2641 tctccacttg ccttaaccat tctctgaagg gtctttaagg cctctccgga atcgatggca
     2701 tcctcggcca tgctcgcccc cttcttcaga gtggagacga ggccggatac gtagatcgcc
     2761 gccccggagt tgatgacgat gatatcccgg gctgctgagg tctctcctct caatacctcg
     2821 actaacctct tggcattctc atcgggcata ccgccggcga tatcagcgct ctcggcctgc
     2881 gggtagccca aatcctcagg acggagggtg taacttttta ccgatccgtc cttgagctca
     2941 gagacgcggg tttggccggt gttggtgatc tcgtccatgc cctcgccgtt gaccaccatc
     3001 gctctcttcg cccccaggat ttgcagcaca taggccagct tctcgcatag gcttggatcg
     3061 tagaccccca tcacctgcgc ctttgccccg gctggattgg tgaggggccc caggatattg
     3121 aagacggtgc gaattcccaa ttcccttctc accggaccca cgcgcttcat ggcggggtgg
     3181 aatgccggcg ccagcatgaa tcctattccc acatcctgaa tcagagactg gacctccgcc
     3241 ggatcggagc tgatccgcac ccccagagcg tccaggacgt tggcgctgcc gcactttgag
     3301 ctgatggcat agttgccgtg cttggccacg ggcacaccgc aggcggcggt gacgatggcg
     3361 gctgcagtgc tcacattgat ggtgttggag ccgtctcctc ctgtgccgca ggtatcgacc
     3421 agcgtgccgc tgacagaggg ctctatgcgg atggcggact ctcgcatctt cctggcaaat
     3481 cccgcgatct cggataccga ttcgcccttt cttcgcaatg ctaccagaac tgctccgatc
     3541 atggcatcgg atgccttatt gaatatctcg tccatcaggg cctcagactc ctcctgggat
     3601 aggtcctgcc cttccatgat cttttgaaga tagctcaaat gaatcaccag atcgattgat
     3661 gtacaacttt gtactttact gagtaaaaac gtacattata tttatagatt atgcattgct
     3721 ggccttatgg a
//
LOCUS       DAOSGP010000123         3720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_22846_length_3720_cov_19.465211, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000123 DAOSGP010000000
VERSION     DAOSGP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22846_length_3720_cov_19.465211"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /gene="pyrE"
                     /locus_tag="PLV49_04500"
     CDS             complement(<1..512)
                     /gene="pyrE"
                     /locus_tag="PLV49_04500"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOG98138.1"
                     /translation="MNDRDRLLELLIERSLEIRPVTLSSGRKSDYYIDCKRVTLSPEG
                     AYLTAKLMLEQIHPQVSAIGGLTLGADPIVSSISVLSHLQGRGLAALIVRKEPKKHGT
                     MSYVEGPALEKGSKVAVVEDVVTSGASLLRAIDRIAAAGYEPVQALTILDREEGGREV
                     IEERGFSLQAL"
     gene            complement(610..1404)
                     /locus_tag="PLV49_04505"
     CDS             complement(610..1404)
                     /locus_tag="PLV49_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG98139.1"
                     /translation="MSAPDSDSPAYSIAPVFGAPLLLAEGKERLLIASDLHLGLEHEL
                     WLGGVSIPSQTRTILALLKGYLDMIMPDRLLILGDLKHNVPKTSWQEKREVPDFLRQL
                     SAQVRVDIVSGNHDSNLADMAPLGVRVHPSTGIVREGRGYFHGHTWPEEKVVRSGFMV
                     AAHLHPAVRLKDPLGNFQTHPVWARAPIQTEVVEEHYGFASSGEIIIMPAFNPLCGGL
                     ALNEPAEDMRGPLLAMANMEHARLYLLDGTDLGLLAEIKAAGDWPI"
     gene            complement(1401..1838)
                     /locus_tag="PLV49_04510"
     CDS             complement(1401..1838)
                     /locus_tag="PLV49_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOG98140.1"
                     /translation="MADMGCRHKIYLSGPLFSAGEIAWGRELSSFLRERLANAGVQVI
                     WPYELSINQLEPEEIFGENLRALDRCDLMVAILDGPLVDDGTAWEIGRFFLQGKKVLG
                     IRTDIRKAGESERSRVNLMIEFSCLSISSNIDELYSELERLLD"
     gene            complement(1848..3347)
                     /gene="lysS"
                     /locus_tag="PLV49_04515"
     CDS             complement(1848..3347)
                     /gene="lysS"
                     /locus_tag="PLV49_04515"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOG98141.1"
                     /translation="MSIHWADVIAEKLEGSGPHTIATGITPSGPVHIGNMREVMTAEA
                     VYRALLDRGVQARLIYIADTFDRLRRLYPFLPESFNEHIGKPLSQIPCPEGCCASYAD
                     HFLTPFLKSMERLGIKPEVYRADVLYKEGKYNDAIKTAFSRKDDIARILKEVSGRDVP
                     EGWSPFDAICKVCGRTNTTHVTGFDLDAETVDYVCSCGSSGTVSMAGGGKLVWRVDWP
                     ARWPIFRVTVEPFGKDHATAGGSYDTGKRISEEIYGYPAPFPMVYEWIHLKGVGAMHS
                     STGIVVTIQEMLDVVPPEVLRYLIIRNKPEKHIEFDPGLPLLSLVDEYDQRKGDERAI
                     ELSNVSKSGPFEIPFRHMVTAVQIARGDEEQLFVALKRSGYDVISRREEILGRARNVL
                     VWLEKYAPKYVKFQLQETLPAAVNNLSPAERQALGLLAGRIEDKTAAEIHDLVYVVAK
                     EMNLDSKKFFQAIYLTFLGDRQGPKVGWFLASLDRDFVRSRLAAAASPA"
     gene            3455..>3720
                     /locus_tag="PLV49_04520"
     CDS             3455..>3720
                     /locus_tag="PLV49_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOG98142.1"
                     /translation="MPSIERPGLDEYFMEIAIVVAKRSTCLRNQVGALFVKNKRILTT
                     GYNGAPAGLEHCDVVGCAREGVQSGTRHELCRAVHAEQNAIIQAA"
BASE COUNT          764 a         1101 c         1051 g          804 t
ORIGIN      
        1 agcgcctgca gggagaagcc gcgctcttcg ataacctccc gtccgccctc ctcgcggtcg
       61 agtatggtca gtgcctgaac cggctcatag ccggcagcgg cgatccggtc aatggctctt
      121 aatagtgagg ctccggaggt taccacatcc tccaccaccg ctacctttga ccccttctcc
      181 agcgctggcc cttccacata gctcatggtg ccatgcttct tgggctcctt tcgcacgatc
      241 agcgctgcca gccctctgcc ctgcagatgg ctcaggacgg atatggatga gacaatgggg
      301 tctgctccca aggttagccc gccgatggcc gatacctgcg gatggatctg ctccagcatc
      361 agcttggcgg taagataggc cccttccggg ctcaatgtga cccttttgca gtctatgtag
      421 tagtcgctct tcctgcccga ggagagagtg accggcctta tctccagcga tctctcaatt
      481 agcagctcga ggagtcgatc tctatcattc actttcaaat ccctcttatt ctgaatgcat
      541 agctgaggaa gaggtgataa tccttgctgt cctgaattga tagatgccgg tcaaatcgat
      601 ccatgcgggt cagattggcc aatccccagc cgcctttatc tcagccaaga ggcctagatc
      661 ggtcccatcc agcagataga ggcgtgcgtg ctccatattg gccatcgcca ggaggggacc
      721 acgcatatcc tctgccggct cattgagggc cagtcctcca caaagaggat tgaaggcagg
      781 catgatgatg atctccccag aagaggcaaa accgtaatgc tcctctacca cctccgtttg
      841 tatgggcgct ctggcccaga ctggatgggt ctggaagttg cccaggggat ccttgagcct
      901 cactgcaggg tgaagatgag ccgctaccat aaacccagaa cgcacgacct tttcctccgg
      961 ccaggtatga ccatgaaagt agcccctgcc ttcccgcaca attccagtgg agggatggac
     1021 tctaaccccc aggggggcca tgtctgctaa gttgctgtca tggtttcccg agactatatc
     1081 caccctcacc tgggcagata gctgacggag gaaatcaggg acctctcgct tctcctgcca
     1141 gctcgtcttg ggcacattgt gcttcaggtc ccccaaaatc agcagcctat caggcataat
     1201 catgtccagg taccctttca ggagagcgag gatcgttctg gtctggctag gaatggatac
     1261 tcctcccagc cacagctcat gctccaggcc caggtggagg tcggaagcaa tgagaagccg
     1321 ctcctttccc tctgccagga ggagaggggc gccaaagact ggagcaatgg aataagcagg
     1381 agagtcagaa tccggagcgc tcaatccagc agcctctcta gctcggaata gagctcatcg
     1441 atattcgagg atatgctcag gcaggagaat tctatcatca agttgacccg ggatcgttca
     1501 gactcccccg cttttctgat atccgtcctt attcccagaa cttttttgcc ctgaaggaag
     1561 aatcggccga tctcccaggc agtgccgtca tccactaaag gcccgtccag gatggccacc
     1621 atcagatcgc agcggtccag agctctcagg ttctccccga agatctcctc cggctccaac
     1681 tgatttattg atagctcata gggccatatg acctgaacgc cggcatttgc cagcctctcg
     1741 cgcagaaagg aagagagctc tcttccccag gcgatctcgc ccgcggagaa aaggggaccc
     1801 gatagataga tcttatgccg gcagcccata tccgccatcg ttcttcctca ggcaggggaa
     1861 gcggccgctg ctagcctgga cctgacaaaa tccctgtcca ggctggccag gaaccagccc
     1921 acctttggac cctgccgatc tcccaggaat gtcaggtata tcgcctggaa gaacttcttg
     1981 gaatccaggt tcatctcctt ggccacgaca tagaccagat cgtggatctc ggctgcggtc
     2041 ttatcctcta tccttccggc gagcaggccc agggcctggc gctcggcggg ggagaggttg
     2101 tttaccgcag caggaagcgt ctcctgaagc tggaacttga catatttggg agcatacttc
     2161 tccagccaga ccagcacgtt ccttgctcgg cccaagatct cctcccttct ggatatgaca
     2221 tcatagccgg atctcttcag ggcgacgaag agctgttcct catctccccg ggcgatctgg
     2281 acggcagtga ccatatgcct gaatggtatc tcgaaggggc cgctcttgct gacgttggag
     2341 agctctatgg ccctctcatc cccttttctc tggtcgtact cgtccaccag gctcaatagc
     2401 ggcaggccgg ggtcgaactc gatgtgcttt tccggcttgt tccggatgat caggtaacgc
     2461 aggacctcgg gcgggacgac gtccagcatc tcctggatgg tgactacaat cccggtggag
     2521 ctgtgcatgg cccccactcc cttgaggtgg atccattcgt agaccatggg gaaaggagcg
     2581 ggataaccat agatctcctc gctgatcctc ttgccggtat cgtatgagcc gcccgcggtg
     2641 gcatgatcct tgccgaaggg ctccacagtc acccggaata tgggccagcg agctggccag
     2701 tccacccgcc agactagctt tcccccgcct gccatggaga cagtgccgct gctgccgcag
     2761 gagcagacat agtccacggt ctcggcatcc aggtcgaatc ccgtaacgtg agtggtgttc
     2821 gtccggccgc agaccttgca gatggcatca aacgggctcc agccctcggg gacgtcgcgg
     2881 cccgagacct ctttcagtat gcgagctatg tcgtccttcc gggagaaggc ggtcttgatg
     2941 gcatcgttgt atttgccctc tttgtagagg acatctgccc ggtagacctc cggttttatg
     3001 ccgagccttt ccatgctctt caggaaagga gtgaggaaat ggtcggcata gctggcgcag
     3061 cagccctcag ggcagggtat ctgggagagg ggcttgccta tatgctcatt gaagctctcc
     3121 ggcaggaagg ggtagagccg gcgcagccgg tcgaaggtgt cggcgatgta gatcagacgc
     3181 gcttgtaccc ctcggtccag gagagcgcga tagacggcct ctgcggtcat gacctcgcgc
     3241 atgttgccga tgtgaaccgg tccggagggg gtaatgccgg tggcgatggt atgggggcca
     3301 gagccttcca gcttctcggc gatgacatcc gcccagtgaa tagacatgtt ccgcaatcga
     3361 tgcaaggagg agatattaag cttccgttgt catcctctga gggccagaag tctccaagag
     3421 agcttattaa gcaagagggc agctatccag gctgatgccc tctatcgaac gtcccggcct
     3481 ggatgagtac tttatggaga tagccatagt ggtggccaag cgctccacct gtctgcgtaa
     3541 ccaggtggga gctctctttg tgaagaataa gaggatcctg accacggggt ataacggcgc
     3601 gcctgcgggt ctcgagcact gcgatgtggt gggttgcgcc cgggaaggtg tccagagcgg
     3661 cacccgccac gaattatgcc gggcggtgca tgccgagcag aacgccatca tccaggcggc
//
LOCUS       DAOSGP010000124         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_23023_length_3700_cov_20.072702, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000124 DAOSGP010000000
VERSION     DAOSGP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23023_length_3700_cov_20.072702"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..562)
                     /locus_tag="PLV49_04525"
     CDS             complement(110..562)
                     /locus_tag="PLV49_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOG98143.1"
                     /translation="MNYSLTPAGRSSRAMGMELHISPKHAREICRTLRGMRANRARDY
                     LEDVIALKRAVPFKRYNRNVAHRHGLVGWDAGRYPEKAAREVLKVLDNAIANGEYKGM
                     ESEKLRIYHAGTLTGRTIQGWMPRAMGRATPKNTQTVTVEMVLTEVKS"
     gene            complement(581..1000)
                     /locus_tag="PLV49_04530"
     CDS             complement(581..1000)
                     /locus_tag="PLV49_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOG98144.1"
                     /translation="MIVLAKKAGSKLPKRKEEFLYRGRKMADLAKLSIEELAGLFPAR
                     QRRSIKRGLAKENKNLLANLKNKDSVRTHIRNMLILPDMVGKNIEIYNGKSFEKVEVM
                     PEMIGHFFGEFAMTRGRVQHGAAGVGATRSSKYVPLK"
     gene            complement(1001..1714)
                     /locus_tag="PLV49_04535"
     CDS             complement(1001..1714)
                     /locus_tag="PLV49_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOG98145.1"
                     /translation="MGHRIISQNRGAGGPRYRAPSHRYRADIKHLKRDQNETVKGIVQ
                     DIMRDPARTAPVGLVTLTSGEMRYILVPEGVCIGQEIACGISAPIEPGNTLPLSEIPE
                     GIPICNVESQPGHGGQFARSSGVCAILVAHDVGQTVVQLPSGEMKWLNPKCMATIGVV
                     AGGGRLDKPLVKAGKSFFKYRSKPKNWPVVRGVAMNAVDHPFGGGGRQHPGRPKTVSR
                     NTSPGRKVGSIAARRTGKR"
     gene            complement(1725..1973)
                     /locus_tag="PLV49_04540"
     CDS             complement(1725..1973)
                     /locus_tag="PLV49_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOG98146.1"
                     /translation="MIIKSPYVTEKVTSMIDSDDTLEFLVNTNATKPQIKKALEDLYD
                     VDVLAVRTMITSQGQKKAVVKLAGEGSANELATRLGLL"
     gene            complement(1970..2743)
                     /gene="rpl4p"
                     /locus_tag="PLV49_04545"
     CDS             complement(1970..2743)
                     /gene="rpl4p"
                     /locus_tag="PLV49_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOG98147.1"
                     /translation="MKTNVIDLSGNAAGEIELPAVFEEEYRPDIIKRAVLAAQANRLQ
                     AYGPHFYAGMNTSAVSWGTGHGTSRVLRLKNGRRAAGVPMAVGGRRSHAPQPNAVYSE
                     KVNKKERRKAIRSAIAATASSGLVEARGHKFSRELPVVAKNDLETLSKTSDIIKFLMA
                     AGLWDDVLRAKAGRHIRAGKGKLRGRKYKGKKSLLIVAGNDAGLGKAARNLPGVDFVT
                     VDMLNAELLAPGTRAGRLTVWTESSLEKLAGKGSPEANQ"
     gene            complement(2747..>3700)
                     /locus_tag="PLV49_04550"
     CDS             complement(2747..>3700)
                     /locus_tag="PLV49_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOG98148.1"
                     /translation="KRAKSEVPRIRSWPEEDKARIAGFAGYKAGMTHVMMIDDRPRSL
                     TEGMEISTPVTVLEVPPMNVVAVRAYESYNGGLRPAGELWAENLSPELSRAMTVPKKT
                     RGTAPGDLVALGDDIADVRVLVHTNPTLVSGIPKKVPEIMEMPINGGSMNDRLSFAQT
                     LLGQQVPISSVFELGDLMDTSAVTKGKGTQGPVRRWGIAMAKRKHARTGKVRHVGNLG
                     PWHPAHISWRVPQLGQMGYHQRTEYNKRLMFIGTDGSKITPDGGFPGYGVIKNQYILI
                     KGSVPGPIKRLVRMRHAMRPGKNFVKAPELLHVSQESKQGV"
BASE COUNT          624 a         1132 c         1012 g          932 t
ORIGIN      
        1 cggttcatct gcatgccgcc gtatcctgct ctctccagct catcagccag atactcattg
       61 accagggcct tggttatgcc ctcggccacg aacttcttct cgacggacat cagctcttca
      121 cctcggtcag gaccatctcc acagtcacgg tctgggtgtt cttgggggtg gctcttccca
      181 tggcccgggg catccaaccc tggatggttc tgcccgtgag agtgccggca tggtagatcc
      241 ttagtttctc tgactccatg cctttgtact cgccattggc gatggcatta tccaggacct
      301 tcaggacctc tcgtgccgcc ttctcgggat atctgcctgc atcccagccc accaggccgt
      361 gcctgtgggc tacattcctg ttgtatcgct tgaaaggaac agccctcttc aaagcgatca
      421 catcttccag atagtctcgg gcacggttag cccgcatgcc tcttagcgtc ctgcagatct
      481 cgcgggcatg ctttggtgag atgtgaagct ccatccccat ggcccttgaa gagcgtcctg
      541 caggcgtaag cgaatagttc aatctgccca atctcacacc tcacttcagc ggcacatact
      601 tgcttgatct ggtagcgccc acgcctgcgg ctccgtgctg gacgcgaccg cgcgtcatag
      661 cgaactcgcc gaagaagtgc ccgatcatct cgggcatgac ctctaccttc tcaaagctct
      721 tgccgttata gatctcgatg ttcttgccca ccatgtccgg cagaattagc atgttccgga
      781 tgtgggtccg aactgagtct ttgttcttca ggtttgccag gagattcttg ttctccttag
      841 caagacctct cttgatcgat ctgcgctgcc gcgctgggaa cagcccggca agctcctcta
      901 tgcttaactt ggcgaggtcg gccatcttcc ggccccgata caagaactct tcctttctct
      961 tgggaagctt ggatcctgct ttctttgcca aaactatcac ctagcgctta ccggttctcc
     1021 gtgcggcgat cgaaccgacc tttcggccgg gagatgtatt cctgctgacc gtcttgggcc
     1081 tgcctggatg ctgacggccg ccgccgccga agggatggtc tacggcgttc attgccaccc
     1141 ctctgaccac gggccagttc ttgggcttgg agcggtactt gaagaagctc tttcctgcct
     1201 taaccagggg cttgtccaga cggccgcctc ccgctaccac tccgatggtc gccatgcatt
     1261 tcgggttgag ccatttcatc tccccgctgg ggagctggac gactgtctgg cctacatcat
     1321 gagccaccag gatggcacag actccggagg agcgggcgaa ctggccgcca tgcccgggct
     1381 gcgactcgac gttgcagata ggtatgccct caggtatctc cgagagaggg agagtatttc
     1441 ccggctcgat tggcgcggat atgccgcagg ctatctcctg gccgatgcag accccctcag
     1501 ggaccaggat gtacctcatc tcgccgctcg tcagggtcac caggcccacg ggagcagttc
     1561 gagctgggtc tcgcataata tcctggacga tgcccttgac ggtctcgttc tgatctcttt
     1621 tcaggtgctt gatgtctgct ctgtaccggt gagagggtgc cctataccta ggacctcctg
     1681 cgcctctatt ttgagatatg attcgatgtc ccattgtatt acacctacag cagtcctagc
     1741 cttgtggcca actcgttcgc gctgccctct ccggccagct tcactaccgc cttcttctgt
     1801 ccctgagagg tgatcatggt cctgactgcc aggacatcga catcataaag atcctccagg
     1861 gcctttttga tctgaggctt ggtggcattt gtgttcacca ggaactccag ggtgtcgtcg
     1921 gagtcgatca tgctggtgac cttttctgta acataggggc tcttgataat cattgatttg
     1981 cctccgggga gccttttcct gccagcttct caagggatga ctctgtccat acggtaagtc
     2041 tgccggcccg ggttccaggg gcgagcagct ctgcgttcag catgtcaacg gtgacgaagt
     2101 caacccctgg aaggttcctg gctgcctttc ccaggcctgc gtcgttgcca gcgacgatga
     2161 gcaagctctt tttacctttg tatttccttc cccgcagctt tcccttgccg gcacggatgt
     2221 gcctccctgc ttttgccctc aggacgtcat cccagaggcc cgctgccatg aggaacttga
     2281 tgatatccga ggtcttgcta agggtctcga gatcattctt tgccacgacc ggcagctctc
     2341 gggagaactt atgaccccgg gcctcgacca ggccggatga ggcggtagct gcaatagctg
     2401 agcggatggc ttttcttctc tccttcttat tgaccttctc gctgtagacg gcgtttggct
     2461 gtggcgcatg cgatctgcgg ccgcccactg ccatggggac gcctgctgct ctcctgccgt
     2521 tcttaagcct gagaaccctg gaggtgccgt gtcccgtgcc ccaggagacg gcggaggtgt
     2581 tcatgccggc ataaaagtgc ggcccataag cctgaagcct gtttgcttgg gcagccagaa
     2641 ccgctctctt gatgatatcc ggtcggtact cctcctcaaa gactgctggc agctctatct
     2701 cgccagctgc gtttcccgat agatctataa cattggtttt cattgcttag accccctgct
     2761 tggattcctg gctcacatgg agcagctcgg gagctttcac gaagttcttt cctggcctca
     2821 tggcatgcct catccgcacg agcctcttga tggggccggg gacgcttcct ttgatcagga
     2881 tatactggtt cttgatcacg ccatagccgg ggaatccccc gtccggagtg atcttcgatc
     2941 cgtcggttcc gatgaacatg agcctcttat tatattctgt cctctggtgg tagcccatct
     3001 ggcccagctg gggaacgcgc cagctgatat gggctggatg ccaggggcca aggttgccca
     3061 catggcggac tttgcctgtg cgagcatgct ttctcttggc catggcgatt ccccagcgcc
     3121 tgactggtcc ctgggtgcct ttgcccttgg taacggcaga ggtgtccatc aggtcgccca
     3181 gctcaaatac gctggaaatc gggacctgct ggcccagcag cgtctgggca aaggagagtc
     3241 tgtcattcat cgagccgccg ttgatcggca tctccatgat ctcaggaacc ttcttgggaa
     3301 ttccggatac taatgtggga ttggtgtgca ccaggacgcg gacatcggcg atatcatcgc
     3361 ccagggcgac cagatctccg ggagcggttc ctcgggtctt cttgggaacg gtcatcgctc
     3421 gggagagctc cgggcttagg ttctctgccc aaagctcgcc tgccgggcgt aggccgccat
     3481 tgtagctctc gtaagcgcgc acagccacca cgttcatggg ggggacctca agaacggtca
     3541 cgggggtgct gatctccatg ccctcggtga ggctgcgcgg tcggtcatct atcatcataa
     3601 catgggtcat gcctgctttg tagccggcga agcctgctat cctggccttg tcctcctcgg
     3661 gccagcttct gattctgggg acctcgctct tggccctctt
//
LOCUS       DAOSGP010000125         3663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_23346_length_3663_cov_18.668792, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000125 DAOSGP010000000
VERSION     DAOSGP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23346_length_3663_cov_18.668792"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>1190)
                     /locus_tag="PLV49_04555"
     CDS             complement(5..>1190)
                     /locus_tag="PLV49_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HOG98149.1"
                     /translation="RQKYLGNRGLIALIAFLSAFVPLSTDLYLPALPGMSKYFQVSAE
                     QVNLTLIFFFIVFAAGMLFWGPLSDKYGRRPILIAGLAIYSLAGFLCAISQDINQLIA
                     FRILQAIGGSSASAVAMAMIKDVYDSRNREMILAWVSSMVLISPMAAPVLGALLLGFT
                     SWRGVFVVLGVIGFVALSGSLALVETLNTRYTGTLSQSIGRLAVVSKNPGFLSLLIVF
                     SLLSFSSMAFIASSSYIYIDGFGLSEKLFSLYFAFNALGMIGGPMLYLRLSKRFRRYS
                     IIIAGFISTFLGGLLVYHLGSLQPWIFALCLFPATTMGSCIRTPGANLMLEQQEEDTG
                     SASALMSCFGILMGSMGMTVISQEWSNTIRILGLMNILVGVLCLCLWLLLSNRPFVKQ
                     IP"
     assembly_gap    1191..1290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1291..1745)
                     /locus_tag="PLV49_04560"
     CDS             complement(<1291..1745)
                     /locus_tag="PLV49_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOG98150.1"
                     /translation="MNGIMRIVGISGSPRKAGNTEFLLNEALAVAAEYGFQTERLLCS
                     ELNFDFCSDCGDCSKGKPCPSKDDMPEVLRAMQEAEGIIVASPVYFGNVTAQLKAVFD
                     RTIPLRRQGFRLRNKIGCAIAVGGSRNGGQEKTLESIHAWMHIQGMIVVG"
     gene            1844..2104
                     /locus_tag="PLV49_04565"
     CDS             1844..2104
                     /locus_tag="PLV49_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOG98151.1"
                     /translation="MNAEITSLLEQDVYTQKGIYVGRVDDAVLDPDNGVVSGLALGDV
                     NRDLFDSKGKGIVIPYRWVTAVGDIIIIRHLSRNAKAEPKIS"
     gene            2076..2777
                     /locus_tag="PLV49_04570"
     CDS             2076..2777
                     /locus_tag="PLV49_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HOG98152.1"
                     /translation="MQRLSQKSANWEIYSGLRARPPLVARADGRGFKNILAGFKKPYD
                     AGFASAMASSASGLFEDSGLSPLLAFMFSDEINLIFLAAPFGGRIEKIDSLVAGSLSA
                     ALSLQLAKPVSMDCRTIPLCKAEIREYLIERQNETWRNHVFSYGFYMLQDEGIDPAGA
                     MERLRGMKEHEIHELVFQRGINLAKTPSWERRGIMIYRDEGRILQDWELPLFSSRKGE
                     ELLARIIISRSGREG"
     gene            complement(2752..>3663)
                     /locus_tag="PLV49_04575"
     CDS             complement(2752..>3663)
                     /locus_tag="PLV49_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98153.1"
                     /translation="SLAHPMTRGEGRLNFQKLLIRIIRLNCVNGHYEFLRPLARLTRE
                     YRRFERTVWALNAIIIAIAIYALMQYLGLTTLMKFYYQDSFILSALPAIVSIISGVAL
                     ATFFKRRKKADVFPLLGSELSEKAKTAFDNKDLDSLPMQSLAEELKLSLAGIKPSQIL
                     DRRQLSGRVVTAILLSAVVALLAQGNLVETADLQSIIELKDRAAGIFQEDKPQPDISQ
                     NMNLSENLFGNPSLAVLNENKLPLVLSPGTGAGSIPTNTKPVERVFQQSQAGEAAAVP
                     SELYIESLPPENREIIKNYFTLLSQSD"
BASE COUNT          895 a          888 c          957 g          823 t
ORIGIN      
        1 tttatcaggg aatctgcttt acaaaaggcc tatttgacag gagaagccaa aggcaaagac
       61 acaatacccc caccaggata ttcatcaatc ccaagatccg aatggtattg ctccactcct
      121 gagagatgac ggtcatgccc atgctgccca ttagaatccc aaaacagctc atgagagcag
      181 aggcagagcc ggtatcttcc tcttgttgct ccagcatcag atttgcgccc ggggtccgga
      241 tgcagcttcc catcgtcgtc gccggaaaaa ggcataatgc gaatatccag ggctgaaggc
      301 tgccaagatg gtacaccagc agccctccga gaaatgtaga gatgaagccg gcgatgataa
      361 tcgaatagcg gcgaaacctc ttagatagac gcagataaag catgggcccc ccaatcatcc
      421 ccagggcatt gaaggcgaaa tagagggaaa agagcttctc gctcaagccg aagccgtcta
      481 tgtaaatgta tgaggatgaa gcgatgaagg ccatagagga gaagctgagc agggagaata
      541 cgattagcag agataaaaat ccagggtttt tcgacaccac tgccagcctc ccaatagact
      601 ggctcaatgt accggtatat cttgtattta gcgtctccac cagggccagg ctaccagata
      661 gagcgacaaa tcctatgact cccaggacca caaacacccc tctccaggat gtgaacccta
      721 aaaggagcgc ccccaagact ggtgccgcca tgggtgatat gaggaccatg gaggagaccc
      781 aggccaggat catctcccgg ttgcggctgt cgtacacatc cttgatcatg gccatggcca
      841 ccgccgaggc tgagcttcct ccaatagcct ggaggatgcg aaaggcgatg agctgattga
      901 tatcctggga gatggcgcag aggaatcctg ccagagaata gatggcaagg ccagctatca
      961 gaatggggcg gcggccgtat ttatcgctca gaggccccca gaagagcatt cccgcggcaa
     1021 aaacgatgaa gaagaatatc agcgtgagat tgacctgctc ggctgatacc tggaaatatt
     1081 tggacatgcc gggcagggcc ggaagataga gatcagtaga tagaggaacg aatgcgctca
     1141 gaaatgcgat aagggcgata aggcccctgt tgcccagata tttctgtctc nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccacaacta tcattccctg gatgtgcatc
     1321 caagcatgaa tgctctccag ggtcttctct tggccgccgt ttctggagcc gcccacggcg
     1381 atcgcgcagc caatcttatt cctgagcctg aagccctgcc tgcggagggg aatggtccgg
     1441 tcgaagaccg ccttgagctg ggcagtgacg tttccgaagt agacgggtga ggccacaatt
     1501 atgccttctg cctcttgcat tgccctcagg acctcgggca tatcatcttt actgggacag
     1561 ggtttgcctt tgctgcagtc tccgcagtcg ctgcaaaaat caaaattcag ctctgagcag
     1621 agcagccttt ccgtctgaaa gccatattca gcagctaccg ccagtgcctc attcagcagg
     1681 aactcggtgt tgccggcctt cctcggactg ccggatattc caactattct cattattcca
     1741 ttcacactgc agtagattgc aggagacgaa tatatgcttt tgcggttcga tctgccgatg
     1801 gcaaagttaa tatctaatac cctaatttta ttatgggatt aaaatgaatg ctgagatcac
     1861 atcgcttctg gaacaggatg tctatactca gaaaggcatc tatgtgggca gggtggacga
     1921 tgccgtctta gatcctgaca acggggtggt aagcggcctg gctttaggag acgtcaaccg
     1981 ggatcttttc gatagcaagg gcaaagggat cgtcatccct taccgctggg ttactgctgt
     2041 cggcgacatc ataataatca ggcacctgag tagaaatgca aaggctgagc caaaaatcag
     2101 ctaactggga gatctattcc ggtcttcggg ctcgtccccc tctggtagca agggcagatg
     2161 gccggggatt caagaatatc ctggctggtt tcaagaagcc ttacgatgca ggcttcgcga
     2221 gcgcaatggc ctcctcagcc agcggcttgt tcgaggattc ggggctcagt cctctcctgg
     2281 cattcatgtt ctccgatgag atcaatctca tcttcctggc tgctcccttt gggggacgga
     2341 tagagaagat agattccctc gtcgcgggct cattatctgc tgctctctct ctccagctag
     2401 caaagcccgt ctcaatggac tgccggacca ttcccctgtg caaggccgag atcagagaat
     2461 acctaattga gcggcagaat gagacctgga gaaaccatgt cttctcttat ggattttata
     2521 tgctgcagga tgaaggaatc gatccggccg gagccatgga gaggctgcgg ggaatgaaag
     2581 agcatgagat tcatgagctg gtcttccaga gaggaataaa cctggccaag actccttcct
     2641 gggagagaag gggaataatg atctatcgcg atgaaggaag aatcttgcag gattgggagc
     2701 tgcctctctt ctcttctcga aaaggggagg agctgctggc ccggattatc atcagtcgct
     2761 ctgggagaga agggtgaagt aattctttat aatctctcta ttctcaggag gaaggctctc
     2821 aatatagagt tctgacggca ctgctgctgc ctctcctgct tgagactgct gaaagaccct
     2881 ctccacgggt ttggtatttg tggggatgga gcccgcgccc gttccaggag ataggactaa
     2941 aggaagctta ttctcgttca ggacggcgag agatggattg ccaaataggt tttcgctcag
     3001 attcatgttt tggcttatat ccggctgggg tttatcctcc tggaagatgc cggcggctct
     3061 gtccttcagc tcgatgatcg actggagatc tgcagtctcg accagattcc cctgcgctaa
     3121 gagcgccacc actgctgaaa ggaggatggc agtcaccact cttccgctca gctgtcttct
     3181 atccagaatc tgggagggtt tgattcctgc caaactcagt ttgagctcct cggccaggct
     3241 ctgcatggga agagaatcaa gatctttgtt gtcaaaggcg gttttagctt tctcagataa
     3301 ttcggagccc aaaagcggaa agacgtcagc cttcttgcgt ctcttgaaaa aggtggctag
     3361 agcaactcct gaaattatgg agacaatggc aggcagtgcc gataggataa agctgtcctg
     3421 atagtagaat ttcataagag tggtcaatcc cagatactgc atgagggcat agatcgcgat
     3481 ggcgattata atggcgttaa gagcccatac tgttcgctca aaacggcgat attccctcgt
     3541 cagtcgagcc agtggtctta ggaactcata gtgcccattc acacagttta atctgatgat
     3601 gcggataagt agtttttgga agttcagtct gccctccccc ctggtcatcg gatgagcaag
     3661 gga
//
LOCUS       DAOSGP010000126         3630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_23639_length_3630_cov_13.609231, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000126 DAOSGP010000000
VERSION     DAOSGP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23639_length_3630_cov_13.609231"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..663)
                     /locus_tag="PLV49_04580"
     CDS             complement(325..663)
                     /locus_tag="PLV49_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG98154.1"
                     /translation="MTEFVNPFSGKVPDRKLTSEELIRALRLDLAAEHEAVHLYMAHA
                     EATDNPLVKEVLIDIANEERVHAGEFSRLLQILTGDEDELLAQGADEVNKMAGKKPSE
                     EMKKPAKKGK"
     gene            complement(871..1338)
                     /locus_tag="PLV49_04585"
     CDS             complement(871..1338)
                     /locus_tag="PLV49_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98155.1"
                     /translation="MIFNRKSKDSKVKAEVYINYGDENGSVNGQKRIKKAEVLINPGM
                     SVLEILKMVSNIEYTPDESATGHMGSMITAIDGFKNDLTHFWLYYLREENDKGWMLPM
                     ETPDSVLIKKNTMIAWRYHDLQEKGPGAASNQIFGPFYSKECIARIKKCSRQF"
     gene            1607..2908
                     /locus_tag="PLV49_04590"
     CDS             1607..2908
                     /locus_tag="PLV49_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98156.1"
                     /translation="MERIIGLILFAALVNSASGLDFRYNETLDSRMPVTEFPETENSD
                     VYVFQQGQSIIAETADGRQIATSSIKSDASGLFKAAIDAVPDGGSLNIGKGLYVLSAP
                     YAVPLYPNGSNILYCCIPVIDKSMHIYGAGVDETIIQLAPNQRNLSRHVAMMIIRGSG
                     PLSPGYSDFTVANLTLDGNRAQQRDEQPQDGEALILAGSKRSNGKYYRLKLVNSHGSG
                     IYLGNNGSGRESDAVVFQVVAQNCGAEGFMLDTCHNSHVLDCAAWQCREGLCLHGNND
                     WKSRSKDQVSATKFQTDSQITVWQVNDFTLSEINMDCTSATDAYGLVIRDGTGQVRRS
                     VLKNDKAKTNSTGGATYIYESSRVLFDDCQLEGFFGIHAVGRSRVEAKNCRLVAPGGC
                     FCTTDPNPVQSTIIAENCVWSEKKVVLQAGAAFLEAGYVEE"
     gene            complement(3045..3530)
                     /locus_tag="PLV49_04595"
     CDS             complement(3045..3530)
                     /locus_tag="PLV49_04595"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOG98157.1"
                     /translation="MIRDSMAEITVIDEKPQLVAGMRRKGHYREIAEMLASLFEYAMS
                     CKAIIVGHPLFLYHEKSVEEAKRADEAGNADIEVCVPILKSIPENDRIRCYELAGGKM
                     AKTIHKGPYQECELTYIELFDWLQKNNHSIVGPIREGYVNDPREVRPEEILTIIYVPI
                     S"
BASE COUNT          915 a          884 c          829 g         1002 t
ORIGIN      
        1 ttcgctatat ggtgtgggca aggaatgctt tgtttctcta tgcgcaactc tgcgactctg
       61 cggttttact tcgcaaacat tagttataac cagtgtcttg agaccattta accgcagagg
      121 cacagagcgc acagaggact acggaaaaat tgttggattc ctgcgccagg cgcatgaacc
      181 cacagaaaac agcgatgagc caaattttag atataggaca aaactggggg agaatagccg
      241 cgcagccatg ttgatttcct gactcgcggc ccttctgcct gaaatcagat tgcacaactt
      301 tgcagcgcta cctgaaaagt tatgttattt acctttctta gcaggttttt tcatctcttc
      361 ggatggcttt ttccctgcca tcttgtttac ctcatctgct ccctgtgcca gcagctcatc
      421 ctcgtccccg gtgaggatct ggagcagacg ggagaactct cctgcatgaa ccctctcctc
      481 attggctata tctatcaata cctccttgac cagaggatta tctgtagcct cggcatgagc
      541 catgtaaaga tgcactgcct catgctctgc agccagatcg aggcgcaaag ctcggatcaa
      601 ttcctctgaa gtcaattttc ggtcaggcac ctttccgctg aatggattga caaactcggt
      661 catttaaaac tcctcttttt atgctgtcca gtttggtatt caagcaactc gagaattcat
      721 ctatctctat cagttaaatt ttcttttaaa gcaaataagc ttttgggttg aatgcctggc
      781 caattaaacg cactttttgt caaagggatg acaaccccta caagagccgc caaccttgcc
      841 tgaggatcct taggttctgc cagaataaga tcagaactgc ctgctgcatt tcttgatcct
      901 ggcaatgcat tccttggagt agaaagggcc gaatatctga ttgctggccg cgccggggcc
      961 cttctcctgc aagtcatgat accgccaggc aatcatggta ttctttttta ttaaaacaga
     1021 atccggtgtc tccatgggta gcatccatcc tttgtcgttc tcctctctta aataatacag
     1081 ccagaagtga gtgaggtcat ttttaaaacc gtcaatggct gttatcatag accccatgtg
     1141 gccggtagcg ctttcatccg gagtatactc gatatttgaa accattttga gaatttccag
     1201 aacgctcatg ccgggattga tcaatacctc tgcctttttt atcctctttt ggccattgac
     1261 acttccattt tcatcgccat agttaatgta tacctctgcc ttgaccttgc tgtcttttga
     1321 ttttctattg aagatcattc tgccacctgt ggccaataca taaatcctga tatatttgag
     1381 ccattgggat tgaatgctca ccaggagcgc tatatcaatc tgcccgttgg gggctctgac
     1441 aaccaggacg gccgtataat tagtgaggta tcaaagagat ggatgcccaa ggacatattg
     1501 catagaacaa cgccatcccg aataacaata tttataacta gtgtgtcctg caagactatc
     1561 tcgcgggcca tggctcgagc caaagaagca acgggatgga ttagaaatgg agcgaataat
     1621 tggattaatc cttttcgctg cactggtcaa tagtgcttcg ggactggatt tccggtataa
     1681 cgaaacgctg gacagtcgaa tgcccgttac tgaatttcca gaaacggaga attctgatgt
     1741 atatgttttc cagcagggcc aatccatcat cgccgaaact gccgacggca ggcagatagc
     1801 cacttcctcc atcaaatccg atgcatccgg tctatttaag gcagccatcg atgccgtccc
     1861 tgatggcggc agcttgaaca ttggcaaggg cctttacgtc ctatctgcgc cctatgccgt
     1921 cccgctatat cccaatggaa gcaacattct ttattgctgc atccctgtaa ttgacaaatc
     1981 catgcacatc tacggcgcgg gagtggatga gactatcatc cagctagccc cgaatcaacg
     2041 caatctttcg cggcacgttg ccatgatgat catcaggggg agtgggccgc tttccccagg
     2101 ttatagcgat tttacagtag ccaatttaac tttggacggc aaccgggcgc aacaaaggga
     2161 tgaacaacca caagatggag aggccctgat cttggctggc tcgaagcgaa gcaacggaaa
     2221 atactatcgc ctcaagttag tgaattctca tggctcaggg atctatttgg gaaataatgg
     2281 atcaggcaga gagagcgatg cggtggtttt ccaagtggta gcccaaaact gcggagcgga
     2341 aggattcatg ctggatacct gccataatag ccatgttttg gattgtgcag cctggcagtg
     2401 cagagagggg ctgtgcctgc atggaaataa cgactggaag agtagatcaa aggatcaggt
     2461 ctcagcaacc aagttccaaa ccgatagcca gataaccgtc tggcaggtga acgacttcac
     2521 cctcagcgaa ataaatatgg attgcacatc agcgactgat gcctatggcc tcgtgatcag
     2581 agacggaacc ggacaggtga gaagatcagt cctgaaaaat gacaaggcaa agaccaattc
     2641 gactggagga gcgacatata tctatgagtc ctctcgggta ctattcgatg attgccagct
     2701 ggaaggattt tttggaatcc atgccgtcgg gcggtctcgt gtagaggcaa agaactgccg
     2761 tctagtagca cctggtgggt gcttctgcac caccgatcca aatcccgttc aaagcaccat
     2821 catcgcagag aattgcgtct ggtccgaaaa aaaggtagtt ttgcaggcgg gtgctgcatt
     2881 cctcgaagct ggatacgtgg aagagtgatg gaggcaggct tttcgtggat gtggcttaat
     2941 acgacagtag tccagtcttt cttttttcca ttctctcaat atggtcatga ctacttcaga
     3001 ttcgacaaaa aatttcggtc caggtaggaa tattgctgca accgtcagct tatggggaca
     3061 tagattatgg tgaggatctc ctccggcctg acctctctgg gatcattgac atagccctct
     3121 cttattggtc cgacgatact gtggttgttc ttttgcagcc agtcaaagag ctctatataa
     3181 gtcaattcgc attcctggta aggtcctttg tgaattgttt tggccatctt tcctccggca
     3241 agctcatagc atctgatcct atcattctct gggatgctct ttaaaatcgg aacgcaaacc
     3301 tctatgtcgg cattgcctgc ctcatccgct ctctttgcct cctcaacgct cttttcgtga
     3361 tataaaaaaa ggggatgccc aacaattatg gctttgcagc tcatggcata ttcaaataga
     3421 gaggcaagca tttctgcgat ctccctataa tgccccttcc gtctcatacc tgcaaccagt
     3481 tgaggcttct cgtctatcac ggtaatttcc gccatgctat cacgtatcat tatctgattt
     3541 tatttcggat atatctttcg agctgtcctc tttggagcat tctctttcag agtctgtacc
     3601 atgtctataa atatttgaca atcagaatct
//
LOCUS       DAOSGP010000127         3614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_23791_length_3614_cov_15.663108, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000127 DAOSGP010000000
VERSION     DAOSGP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23791_length_3614_cov_15.663108"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3614
                     /locus_tag="PLV49_04600"
     CDS             <1..>3614
                     /locus_tag="PLV49_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98158.1"
                     /translation="ILPVSAEPNQIIGGVSYTISAPLAPEGQSYSYSWSATGGTPLNA
                     SQSSFQWTAPLVENSTTVLIELKIESNVTGCINENQIEMQVQPAAKAEIRLKKDCRYS
                     PPVRVGDTVVYTYNVTNTGGVTLYEINLTDTHDWGPNCHPTRVGGGGGDEALEPGQSW
                     QYECPYEIHDPANYTILRIMASTGYREQDMIEKLLRLRTRMEINLEKMRRLQKEFDPH
                     AATLTIESMQMAGINYTSYYYVNEVTEESLKRIIDPQGNLNVTYYSDPVSQLNLTTRY
                     DPKGKILSEEEYYYPHPETNEYMKIEYDNPSVGLKTITITDYNTGDTLIIIEDYNGVI
                     ISKEYKKTPGYIVYEEKYILKNIATVKAKTGAGQEVSDSDTYSLEIFKQNPVLSVFKD
                     AEPDPVQSNGTINYTITYKNNGGQYAHEVMLKETYDESLEFIWASPAPDPGTIDTWTI
                     GDLDKEESGKIAIQARLKAILTPGSIITNRIDMWCKEGSHAVAWANTTVTGEGLVIAK
                     RASSRIIVPGEHLNYTIDYRNNGSVKQTDVVIHDYLDEKVDFKYSLSDPLLINESSGR
                     HHWWYVGDMLPGSGGTIEIFVKAKGKDTFKDNADSVFNTYQINSSQTKGTNKTLETSV
                     VHSLWIKKEANATAAARGENITYTIKYGNSDTDFTAENVSITDMLPEVDFLGATPQPN
                     FINGKNLTWMIGNLTPRANGTIVLEVHIPKKAQMVFDETSSVEGDGFVRMRKSLSTEE
                     AERSLTNRVEIRGYYESMPIPVSSSSAVVTILGAAGTRITTSEHGSGHYEEDESSRLV
                     SENKSVSLKKDIFASYGSSTFQLPGKRSIDYQSLWSDRTSAANYIRGEVVTENFMYAD
                     SLQKNSSFDVDMNQTVYRSDANYGSGIARISYRKNIQDSPQSTKEMIDEDYHGSFRVF
                     QSVDSYGENIKYDRSITGKGRVSSDKRTEYQRSFEHGSGYYSAEESMDSTSITKDVKM
                     LYSPQKETLGSMNTSVGGLWQEGMQTKDPELELIISEKIQSASYIEKEAEMDLSSLKF
                     IGSFNGSMDTAILQGIGPDNEKVRLEQSFVGEFNIDTAISLHPEPLHRYVHVNIEKKA
                     IMQDENTVLFLINVTNDGSRPLKKLNVSDRLPLGLSFIDSSQRAEVDGQFINWTISSL
                     DIGRTMTIKLRARVENGRQIYDNYVSVSTGYNDTRIEAENHTLFEAYYQPLSCCPELP
                     "
BASE COUNT         1122 a          807 c          883 g          802 t
ORIGIN      
        1 taatccttcc cgtcagcgca gagccgaatc agatcatcgg tggcgtatcc tatacgatct
       61 ccgcaccttt agcccctgag gggcagagct acagttatag ctggtccgca actggaggca
      121 ctccactaaa cgcgagccag agctcatttc agtggacagc tccccttgta gagaattcta
      181 ccacggtcct gatcgaactg aaaatagaga gcaatgtaac cggctgcatt aatgaaaacc
      241 agattgagat gcaagtccag cccgcggcaa aagcagagat tcgcctaaaa aaagactgca
      301 gatatagccc accagtgagg gtgggcgata cagtcgttta tacatataac gtaaccaata
      361 ccggcggagt cactctgtat gaaataaacc tcaccgatac tcatgattgg gggccgaact
      421 gccatccaac tcgggttgga ggtgggggag gtgacgaagc attggagccg ggccaatcat
      481 ggcaatatga atgtccttac gagattcatg atcctgccaa ttataccata ctgcgcatca
      541 tggctagcac tggttacagg gagcaggata tgattgagaa gctactgcgg ctgagaactc
      601 gcatggaaat caatctggag aagatgagaa gactgcaaaa agagttcgat ccgcatgcgg
      661 caacgctaac tattgagagc atgcagatgg ctggcatcaa ctatacctcc tactactatg
      721 tcaatgaagt gactgaggag agcctgaaga ggattataga tccgcaaggg aatttaaacg
      781 tcacttacta tagcgatccc gtctcgcaat tgaatttgac cactcgatac gatccaaaag
      841 gaaagattct ctcggaggag gaatactact atccgcatcc ggaaacgaat gagtacatga
      901 agatcgaata cgataatcca tctgtgggct tgaagaccat tactattacg gattataata
      961 caggagatac attgatcata atagaagatt ataacggagt tataataagc aaggaataca
     1021 aaaaaacgcc gggctacata gtttacgagg agaaatatat cctcaaaaat atcgccactg
     1081 ttaaggcgaa gacgggagct ggccaagagg tatctgattc agatacatat tctttggaga
     1141 tattcaagca aaatccagtt ttgagcgttt ttaaggatgc tgagccagat ccagtgcaat
     1201 ccaatggtac tattaattat accatcacat ataaaaataa tggcggtcag tatgctcacg
     1261 aggtaatgct aaaagagacc tacgatgaga gcctggagtt catatgggca agccctgctc
     1321 cggatccggg cacaattgat acctggacca ttggagatct ggataaagaa gagtccggca
     1381 agatagcgat ccaggccaga ttgaaagcta ttctcacacc cggatcaata atcaccaatc
     1441 gcattgatat gtggtgtaaa gaaggatcgc atgccgttgc atgggccaat acaactgtaa
     1501 cgggagaagg gctggtaatt gcaaagaggg cctcatcccg cataattgtc ccaggggagc
     1561 atttgaatta caccatagac tacaggaaca atggctctgt gaagcagacc gatgttgtga
     1621 tccatgatta tttggatgaa aaagtcgatt ttaagtattc tctatcagat ccacttctca
     1681 tcaatgagag ctcaggaaga caccattggt ggtatgttgg agatatgctg cccggatccg
     1741 gagggacaat cgagatcttc gtgaaggcca agggcaaaga cacattcaaa gacaacgccg
     1801 attcggtctt caacacatat cagataaact cttcgcaaac caaaggcacg aataaaacac
     1861 tggagacctc cgtagtccac tcattatgga tcaagaagga ggcaaatgcg actgccgccg
     1921 ctcgaggtga gaatatcacc tatacaataa aatatggaaa ctccgacacc gattttaccg
     1981 ctgagaatgt gagcataacg gatatgcttc cagaagttga ttttctcgga gcaactcctc
     2041 agcccaattt cataaacgga aaaaatctta catggatgat cggcaatctg actccaaggg
     2101 caaacggcac gatcgttctc gaggttcata tacccaagaa ggcgcagatg gtcttcgatg
     2161 aaacctcctc tgtagaaggc gatggctttg tccgcatgcg aaagagcctc tccactgaag
     2221 aagcggagcg ctcgctcaca aacagggtcg aaatccgggg gtactatgaa tctatgccaa
     2281 tccctgtcag cagcagctct gcagtggtca ccattctcgg agctgccgga acaaggatca
     2341 ctacttcgga gcatggaagc ggccactacg aggaggatga gagttcgcgc ttggtatcag
     2401 agaacaagag cgtgagtcta aaaaaggata tctttgccag ctatgggagt tctactttcc
     2461 agctgcccgg caaacgaagc atagactacc aatccctgtg gtcggacaga acatcagcgg
     2521 caaattatat tcgaggagaa gtggtcacag agaattttat gtatgctgac agcctgcaaa
     2581 aaaacagcag ctttgatgtg gatatgaacc aaacagtata tcgttcagac gccaactatg
     2641 gttcgggaat agctcgaatc agctatagga agaatataca ggatagccca caaagcacaa
     2701 aagaaatgat agatgaagat tatcatggca gtttcagagt gtttcaatcc gttgactctt
     2761 atggagagaa tataaagtat gataggtcga tcacaggcaa aggacgggtg tcctcggaca
     2821 agcgaacaga atatcaaaga tcctttgagc atggtagcgg ttactattca gcagaggaat
     2881 cgatggattc aaccagcatc acaaaggacg tcaagatgct ctactcgccg caaaaagaga
     2941 ccctgggatc catgaacacc agcgtcggag gcctatggca ggaaggcatg cagactaaag
     3001 atccagagct ggagctcatc ataagcgaaa agattcaatc cgcctcttac attgaaaaag
     3061 aggcagaaat ggatttgtca tcattgaagt tcatagggtc cttcaacggg tctatggata
     3121 ctgcaatcct tcaaggaatt ggcccagata atgaaaaggt gcggctggag cagagctttg
     3181 tgggcgaatt caacattgat accgccatca gtctgcatcc tgagcccttg caccgctatg
     3241 tgcatgtgaa cattgagaag aaagccatta tgcaggatga aaatacagtc ctcttcctta
     3301 tcaatgtcac caatgacggc agcaggccgc tcaagaaatt gaatgtttcg gacaggcttc
     3361 ctcttggcct gagcttcatc gactccagcc agagggcaga agttgatggt caattcatca
     3421 attggactat ctcgtcgctg gacattggca gaacgatgac cataaagctg agagccaggg
     3481 tcgagaacgg ccgccagata tatgataatt acgtgagcgt gtcaaccggc tataacgaca
     3541 ctagaattga agccgagaac cataccctct ttgaggctta ctatcagccg ctctcctgtt
     3601 gcccggagct gcca
//
LOCUS       DAOSGP010000128         3612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_23811_length_3612_cov_17.557492, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000128 DAOSGP010000000
VERSION     DAOSGP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23811_length_3612_cov_17.557492"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLV49_04605"
     CDS             complement(<1..794)
                     /locus_tag="PLV49_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG98159.1"
                     /translation="MDLTPVQRDILTALINIYRVEGRAVKGEEIAELIDRNPGTIRNQ
                     MQSLKALNLVEGVPGPKGGYRATGSAYEALNVEATGDVVTVPVLRNGVLMEGTTASEI
                     IFNKVMHTQLCDGVIRIIGNIRDFNVGDEVEVGPTPVNKLYIRGTVRGRDDTMSRLII
                     HVDAMISVPKLAIKKIARRAVRIPPGASMQEAARILVHNGVQEALVEDSSPGMVNQTD
                     IVRAIADGKGDQEAREFMSRGFLTIDSEDTIYEAIKMLGKTGSGQLV"
     gene            complement(937..2073)
                     /locus_tag="PLV49_04610"
     CDS             complement(937..2073)
                     /locus_tag="PLV49_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98160.1"
                     /translation="MNPAQVDPEPSSDTITGRSYPLYRSPLVRADARLEGGRVKIRVT
                     GPLASLPAIRSSMELFDGQLAVRAGTEQLSLSTWIPPLPGKAFDRLANSRIRALFGLR
                     TPDQVTISITEECPNRCSHCALPNSGNRLRLDPNTVRDLINQVLDMGTTLVIFDGGEP
                     TLYRELPDLVRHVDDRAISTLFTSGAGFTLPLAERLKDAGLYAVNVSLDSPLAEEHDA
                     MRGREGVFSEAMQAVKNALLAGLLVDLYVVVRRENLCHLERFHQLARSTGAHELTLFE
                     VVSTGRWSGREGLALTSAEHAHLAEFVSSRGAPRIFSVPDAYRRFGCFAARSWMHVTP
                     SGEVYPCSCYPESWGNIMKEPMKRIWQRMGRFPHKGEKICPMRK"
     assembly_gap    2075..2174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2175..2491)
                     /gene="hisI"
                     /locus_tag="PLV49_04615"
     CDS             complement(<2175..2491)
                     /gene="hisI"
                     /locus_tag="PLV49_04615"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOG98161.1"
                     /translation="MNERLIPAIAQDWRTGEVLMLAYMNDEALAKTQETGKAHYWSRS
                     RNKLWLKGESSGHFQLVREIRVDCDEDAILLLIEQEGGACHTGYRSCFYRTIDGRVVG
                     EKV"
     gene            2579..>3612
                     /locus_tag="PLV49_04620"
     CDS             2579..>3612
                     /locus_tag="PLV49_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98162.1"
                     /translation="MSCITCKGRGECGQPVCPIVRRLEELVSLPKIGSRMEGFTPPEV
                     FVGRFGYPLVRAGPVLPSARAEELPHLGMNMDEIISARMGMVRSETKVRVMEADEPGR
                     LLEACQQIAMSSAPVGAEVSFIKPPRRRLQFDGVLSPTGPSGELAKMEITTNPLIPRK
                     VDQIVLDRGAPADVAIAELYSAGIDIDHLSRLLSIGLLGNKRRLVPTRWSITASDDMI
                     GKSLKDAVLDFPEVKGYYLFSGEELGNHFEVLLSPRPFSFELIEIWRPHSLWAEEGFI
                     GRDGEDARPKKGYSPLAGGYYAARLAVLEHLSRWGRQAGVLAIREISEDYRIPLGVWV
                     VREVARKAMSS"
BASE COUNT          704 a         1015 c          995 g          798 t
ORIGIN      
        1 accagctggc cagagccggt cttgcccagc atcttgatcg cctcgtatat ggtatcctcg
       61 gagtcgatgg tcaaaaatcc ccggctcatg aactctctcg cttcctgatc cccctttccg
      121 tctgctatcg cccggacgat gtccgtctgg ttgaccatcc caggggagct gtcctcgacc
      181 agtgcctcct gaacgccgtt gtgcaccaga atgcgcgctg cctcctgcat ggaagctcca
      241 gggggaatgc gcactgcgcg acgggcgatc ttcttgatgg ccaacttggg cacggatatc
      301 atcgcatcaa cgtggatgat cagccggctc atggtgtcat ccctgccccg gacggtgccc
      361 cgaatgtaga gcttgttgac aggagtgggt cccacttcta cctcatcgcc cacattgaag
      421 tctctgatgt tgccgatgat gcggatcacg ccatcgcata gctgagtgtg catcaccttg
      481 ttaaatatga tctcgctggc ggtggttccc tccattagca cgccattcct gagaacgggt
      541 acggtcacca catcccccgt ggcctcgaca ttgagtgcct cataggcgga tcctgtggcc
      601 ctgtagcccc ccttggggcc aggcaccccc tccaccagat tgagggcttt cagggattgc
      661 atctgatttc ggatggttcc cggattgcgg tctataagct cggcgatctc ttcgccctta
      721 actgctcttc cttcaacacg atagatgttg atcagcgcgg ttagtatatc tctttgaaca
      781 ggtgtcaaat ccatggttag atcaagattg ttcatgatta atgtcctata tagatctatt
      841 gattgtaggg gacctatgaa aattccgttt aattttaatc ctgctcatgt tcctccttga
      901 acagttaaaa tcttgtttaa tggcagaaaa attgttttat ttcctcatag ggcatatctt
      961 ctcccctttg tgtggaaatc gtcccattcg ctgccagatt ctcttcatcg gttctttcat
     1021 aatgttgccc caggactccg ggtagcagga gcagggatag acctctcctg agggagttac
     1081 atgcatccag cttctggccg caaagcagcc aaagcgtcga taggcgtcgg gaacagagaa
     1141 gatcctgggc gctcccctgg aggagacgaa ttccgccaga tgggcgtgct ccgcagaggt
     1201 cagggccagg ccctcgcgac cggaccagcg gccagtggat accacctcga atagtgtcag
     1261 ctcatgcgct cctgtgcttc tggccagctg gtgaaacctc tccagatggc agaggttctc
     1321 ccggcgaacc acaacataaa ggtccaccaa cagtcccgct agaagggcat ttttcactgc
     1381 ctgcatcgcc tcggagaaga cgccctccct tccccgcatg gcatcgtgct cctcagccag
     1441 ggggctgtcc aggctgacat tgaccgcata aagaccagca tcttttagcc gttctgccag
     1501 ggggagggtg aatcccgctc cagaggtgaa gagggtggat atggctctgt cgtccacatg
     1561 tctcaccaga tccggcagct ctcgatagag ggtcggctct cccccatcga atataaccag
     1621 ggtggttccc atgtccagaa cctggtttat gaggtctcgg actgtgttcg gatcgaggcg
     1681 cagccggttt ccggagttgg gcagggcgca atgcgagcag cggttagggc attcctcagt
     1741 gatagagatt gtcacctggt ccggggtgcg caggccaaag agagctcgga tgcgactatt
     1801 tgccagccgg tcaaatgctt tgcctggcag gggaggaatc caggttgata gggagagctg
     1861 ctcagttcct gcccgcacag ccagctggcc atcgaaaagc tccatgctgg atctgatagc
     1921 gggcaagctg gcaagcggtc ctgtaaccct gatcttcact cgccccccct ccagcctggc
     1981 atctgcccgg accagagggc tgcgataaag gggataggac ctgcccgtga tggtgtcgga
     2041 actcggttct ggatcgacct gggcgggatt catcnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnaagacc ttctccccca ccacccggcc atcaatggtg cgataaaagc
     2221 aggatctata acctgtatgg caggcaccgc cctcctgctc gatcaggagc agtatggcat
     2281 cctcatcgca gtccaccctt atctccctga caagctgaaa gtggccggat gactcccctt
     2341 taagccagag cttatttcgc gatctggacc agtaatgagc cttgcccgtc tcttgggtct
     2401 tggccagagc ctcatcgttc atataggcca gcatcagcac ctctcctgtc ctccagtcct
     2461 gggcgatggc cggaatcaac ctctcgttca tacagaaaaa ttatagcgga tatttcctat
     2521 aagggccttg cccagaaaga agggaaggca tttttcatat gagagtgaac ggagaattat
     2581 gagctgcatc acctgcaagg gccggggaga gtgcggccag ccggtctgcc ccatagtccg
     2641 ccggctggag gagctggtat ccctccccaa gatcggcagt cgcatggagg gattcacccc
     2701 accggaggtc ttcgtgggcc ggttcggcta tcccctggtc agagctgggc ccgtcctccc
     2761 atctgcccgg gccgaagagc tgcctcatct ggggatgaac atggatgaga tcatctctgc
     2821 ccgcatgggg atggtccgtt ccgagaccaa ggttcgggta atggaggcag acgagcctgg
     2881 aaggcttctg gaggcctgcc agcagatcgc tatgtcctct gccccggtgg gtgcggaggt
     2941 ctccttcatc aaaccgcccc ggagaaggct gcagttcgat ggcgtcctct cccccaccgg
     3001 tccatccggg gagttggcta agatggagat caccaccaat cccctaatcc cccgcaaggt
     3061 ggaccagatc gtcctggacc ggggtgctcc ggccgatgtg gcaatcgcgg agctttactc
     3121 tgcaggcata gatatcgatc acctctcccg actgctctcc ataggcttgc tcggcaataa
     3181 gaggaggcta gtgcccaccc gctggtccat aacggcatct gacgacatga tcggcaagag
     3241 cctgaaggat gcggttcttg atttccccga ggttaaaggc tactatttgt tctctgggga
     3301 ggaactggga aaccactttg aagtcctcct ttctccccgg cccttcagct tcgagctgat
     3361 tgagatctgg cggcctcatt ctctctgggc ggaggaggga tttataggcc gggatgggga
     3421 ggacgcccgt cccaaaaagg gctacagccc tctggccggc ggttactatg ccgctcggct
     3481 cgccgtgctg gagcatctct ctcgctgggg tcggcaggca ggggtgctgg cgataaggga
     3541 gatctcagag gactacagga tacctctagg ggtctgggtg gtgagggagg tggcgcgaaa
     3601 ggcgatgagc tc
//
LOCUS       DAOSGP010000129         3600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_23930_length_3600_cov_21.233850, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000129 DAOSGP010000000
VERSION     DAOSGP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23930_length_3600_cov_21.233850"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLV49_04625"
     CDS             <1..276
                     /locus_tag="PLV49_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HOG98163.1"
                     /translation="DVRRLIRCPGSLHGGSGLRVTPLSISGLEEFDPLEDAVVFGDEP
                     VFLEISKPFSTQMKGNSYSLKEGTEELPSCVAVFLMARGVAQARTRH"
     gene            349..915
                     /locus_tag="PLV49_04630"
     CDS             349..915
                     /locus_tag="PLV49_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98164.1"
                     /translation="MDLANALREERKSAELQPLDEDFYQQVGTYLTGMSQELSQIIDP
                     FSVEAQILQDTLKSERSNLNKLIDQRIKKIVRRALRNARSASREEVFSGMTAEETEIY
                     RQMLSTIALGRESILSHMTPTERPLTGKKDILREYEVVRLLDDVPMFAGVDGKNYLLA
                     RDDVVVLPAVHARNLRNKNLASEVKIQR"
     gene            928..1203
                     /locus_tag="PLV49_04635"
     CDS             928..1203
                     /locus_tag="PLV49_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HOG98165.1"
                     /translation="MPKKLNAYCPFCKKHTAQTVERVRKGRASTLTRITRQKLRANGI
                     GNQGKFSKVPGGDKPTKRLNLRYRCGECKKAHNRPGFKAGKFDLVES"
     gene            1206..1373
                     /locus_tag="PLV49_04640"
     CDS             1206..1373
                     /locus_tag="PLV49_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HOG98166.1"
                     /translation="MPSKFVKVKCNDCENEQVIFAHASTVVKCLVCGRTLAEPTGGKA
                     LIKTAVVEVLE"
     gene            1380..2153
                     /locus_tag="PLV49_04645"
     CDS             1380..2153
                     /locus_tag="PLV49_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HOG98167.1"
                     /translation="MQREGWPEPGSLVVCTVTQVVDFGAFVSIDEYAGKQGLIHISEV
                     ASGWIKYIRDHVREGQKIVCKVLNVDTSRHHIDLSLKDVNEHQRREKIQEWKADQKAW
                     KWLEMAYKDRPEDAKRVADSLMAAYDSLYLAMEEAAISGAEALNEADLTEEDVKILES
                     LSRDNVKIPSVDISGYVDITSPAPDGVEVIRKALKQARRTKDKDATLSVSYIGAPRYR
                     IHVTAPDYKHAESVLKKAAQAIVSYIEQHGGEGAFHREA"
     gene            2150..2311
                     /locus_tag="PLV49_04650"
     CDS             2150..2311
                     /locus_tag="PLV49_04650"
                     /inference="COORDINATES: protein motif:HMM:NF009623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HOG98168.1"
                     /translation="MRSKILRCESCARYTLKEICPACGGRAAPTKPARFSPDDPYGRY
                     RRALALEAE"
     gene            2308..3078
                     /locus_tag="PLV49_04655"
     CDS             2308..3078
                     /locus_tag="PLV49_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HOG98169.1"
                     /translation="MKETLVFRIKDVDLTDPIMVEGLPGVGHVGKLVADHMVEELHAE
                     KIIEIYSPHFPPQVMVKEDGTIKQVKNEIYARRGQNGEPDLLIIIGDYQSATNEGHYE
                     LTSIFLDIAESYKVRRIYALGGYGTGQFVDKSSVMGAATSIELVEEMKTQGVLFQENE
                     PGGGIIGVSGLLLGLGALRGLDVICLMGVTSGYLVDPKAASEVLRVLSGILGIEVGMH
                     ALEERAKEMEKIIGKLQEMERAQSPFEAGGDEDLRYIG"
     gene            3053..>3600
                     /locus_tag="PLV49_04660"
     CDS             3053..>3600
                     /locus_tag="PLV49_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOG98170.1"
                     /translation="MRISGTSDSLIESAHRLLSLALKEGATEAEVYGMVGRSVDVDLR
                     REYVEMASESYHCGLGLRAVVDGAVGFSSSSDMAQLKFVAMSAVRSARARGRDDTWIA
                     LPQPERVTEPEGIYDRRLDKISPEECIDIAHSLLAGCNEVDGAEPVSGGVACVSGTGF
                     VLNSQGIELVETSTLMQASMEA"
BASE COUNT          873 a          887 c         1119 g          721 t
ORIGIN      
        1 gatgtccgtc ggctgattcg atgccctggg agcctgcacg gcggctcggg gctgagggtg
       61 actcccttat cgattagcgg cctggaggag ttcgaccccc tggaggatgc ggtggtcttc
      121 ggggatgaac cggtttttct ggagatctca aagcccttca gcactcagat gaaggggaac
      181 agctacagcc tgaaggaggg caccgaggag ctgccaagtt gcgtggccgt attcctcatg
      241 gccagagggg tggcccaggc caggacaaga cactagaacg gtgaaaagct attatactat
      301 ccagccactt aagatcagtc cgcataaatt tgatgatgag gtggtgaaat ggatctggcc
      361 aatgctctcc gggaagagcg caagtccgcg gagcttcagc cgttggatga ggatttctat
      421 cagcaggttg gcacttatct gaccggcatg agccaggagc tctctcaaat catagatccc
      481 ttcagcgttg aggctcaaat cctgcaggat accctcaaga gcgagaggag caacctgaac
      541 aagctcatcg accagaggat aaagaagatc gtccgccggg cgcttcgcaa tgcgagatca
      601 gcttctcggg aggaggtctt ctccggcatg acggcagagg agacggagat ctaccggcag
      661 atgctctcga ccattgccct cggccgggag tccatcctct cccatatgac tcccaccgaa
      721 aggcctttaa cgggcaaaaa agatatattg cgggaatacg aagtcgtgcg gttgctggat
      781 gatgttccta tgtttgcggg agttgacggg aagaattacc ttctcgccag ggacgatgtg
      841 gtcgtcctcc ctgccgtcca tgccagaaat ctgcgcaaca aaaatttagc ttcagaagtg
      901 aagatccaga gatgataaga agtgaagatg ccaaagaagc ttaacgctta ctgtccgttc
      961 tgcaagaagc acaccgcaca aacggtggaa agggtgagaa aaggccgggc ttcaaccttg
     1021 acccgcatta ccaggcagaa gctcagagcc aatggaatag ggaatcaggg gaagttctcc
     1081 aaggttccag gcggagacaa gcccacgaag agacttaacc tgaggtaccg ctgtggtgag
     1141 tgcaagaagg cccacaaccg ccccggattc aaggcgggca agttcgatct ggtggagagc
     1201 tagatatgcc cagcaagttc gtcaaggtaa aatgcaacga ctgcgagaat gagcaggtga
     1261 tcttcgccca tgcctcaact gtggtgaaat gcctggtatg cgggcgcaca ttggctgagc
     1321 ccactggggg caaggcattg atcaaaaccg ccgtagtaga agtgctcgag tgatttctaa
     1381 tgcaacgtga gggctggcca gagcccggat ctcttgtggt ctgcaccgtc actcaggtgg
     1441 tggacttcgg tgctttcgtc tccatagacg agtatgcagg caagcagggc ctgattcata
     1501 tctccgaggt agccagcgga tggatcaagt acattcgcga ccatgtgcga gaagggcaga
     1561 agatagtttg taaggtactg aatgtagata cctcccgcca tcacatcgat ctatctctca
     1621 aggatgtgaa tgagcatcag aggcgggaga agatccagga gtggaaggcg gaccagaagg
     1681 cctggaaatg gctggagatg gcctataaag accggcccga ggatgccaag agggtggctg
     1741 attctctcat ggccgcctac gacagcctct atctggccat ggaggaggcg gcgatcagcg
     1801 gcgccgaagc tctgaacgaa gccgatctca ccgaagagga tgtcaagatc ttagagagcc
     1861 tatctcgaga taatgtcaag ataccgtcgg ttgacatcag cggctatgtg gatatcacct
     1921 ctcccgctcc ggatggtgta gaggttatcc gcaaggcttt aaaacaggcc agaaggacca
     1981 aggacaagga tgctaccctc tcagtttcgt atatcggagc accgcgatac cgcattcatg
     2041 taactgctcc tgactataag catgcagagt ctgttctcaa aaaggccgct caggccatag
     2101 taagctacat agaacaacac ggcggagagg gcgctttcca cagagaggca tgagatccaa
     2161 gatcctgcgg tgtgagtcct gcgctcgata caccttaaag gagatatgtc cagcctgtgg
     2221 cggccgtgcc gcgccaacca aaccagcgag gttctcccct gatgatcctt atggcagata
     2281 caggagagcc ctagccctgg aggcagaatg aaagagaccc tggtttttcg cataaaagat
     2341 gtagatctaa ccgatcccat tatggttgag gggctgcctg gggtaggcca cgtgggtaaa
     2401 ttggtagcag atcacatggt agaggagctg catgccgaga agatcattga gatctactct
     2461 ccccattttc ctccccaggt gatggtgaag gaggatggaa ccatcaagca ggtgaaaaat
     2521 gagatctatg cgcgacgggg gcagaatggg gagcccgatc tattgatcat catcggcgac
     2581 tatcagagtg ccaccaatga gggccattac gagctgacca gcatatttct ggacatcgcc
     2641 gagagctaca aggttcggcg aatctatgct ctgggcggct atggtaccgg ccagttcgtg
     2701 gataagtcct cggtgatggg cgcggccacc agcatcgagc tggtggagga gatgaaaacg
     2761 cagggggtcc tcttccagga gaacgagccc ggtgggggca ttataggcgt ctccggcctc
     2821 ttgctgggcc tgggagccct acggggactg gatgtgatct gtctgatggg tgttaccagt
     2881 ggctatctgg tcgatccaaa ggcagcatca gaggtcctgc gcgttctgtc cgggatcctg
     2941 ggcatagagg tgggaatgca tgcccttgag gagagggcga aggagatgga gaagatcata
     3001 ggcaagctcc aggagatgga gcgggcccag tctcccttcg aagcgggtgg ggatgaggat
     3061 ctcaggtaca tcggatagcc tcattgaatc cgcccaccgg ctgctatccc tggccttaaa
     3121 agaaggggca acagaggccg aggtctatgg tatggtggga cgctcggtcg atgtcgacct
     3181 ccgcagggaa tacgtggaga tggcgagcga gagctaccac tgcggtctgg gattgcgcgc
     3241 ggtagtggat ggtgctgtcg gattctccag cagcagcgac atggctcagc tgaagttcgt
     3301 agccatgagc gctgttcgat cggccagggc acggggcagg gatgacacct ggatcgctct
     3361 ccctcagccg gagagggtaa cagagcccga ggggatctat gaccggcggc tggataagat
     3421 cagtcccgag gagtgcatcg acatcgccca tagcctcttg gcgggctgca atgaggtcga
     3481 tggtgcagag ccggtctccg gcggtgtggc ctgtgtctca ggcacaggat ttgtgctcaa
     3541 ttctcagggg atagagctgg tggagacctc gaccctcatg caggcctcaa tggaggccat
//
LOCUS       DAOSGP010000130         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_24110_length_3582_cov_18.055855, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000130 DAOSGP010000000
VERSION     DAOSGP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24110_length_3582_cov_18.055855"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(417..677)
                     /locus_tag="PLV49_04665"
     CDS             complement(417..677)
                     /locus_tag="PLV49_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98171.1"
                     /translation="MRLNNQAKVGLATVVCLLLQGYIFTYVLFVEPHPLVSILPLFPY
                     LAYVYARGKRTWYFNKPLYWIGLVAMVTVLDILPFAVVATRS"
     gene            complement(852..1082)
                     /locus_tag="PLV49_04670"
     CDS             complement(852..1082)
                     /locus_tag="PLV49_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HOG98172.1"
                     /translation="MRMQTKIRMGVYKMVDLLYLAVVFFAIALILGVLGWRGAGISMD
                     IAKWLVIAFIILAILSLVFGGSINLATLLLAF"
     gene            1456..1611
                     /locus_tag="PLV49_04675"
     CDS             1456..1611
                     /locus_tag="PLV49_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98173.1"
                     /translation="MPKTTLTLSEPIAKALKIYTVQAKSSMRAQSQVIEEALREFFEN
                     HGIKVEE"
     assembly_gap    1799..1898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1899..2815)
                     /locus_tag="PLV49_04680"
     CDS             complement(<1899..2815)
                     /locus_tag="PLV49_04680"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOG98174.1"
                     /translation="MRREDSLKDSKTNSKKKLTPLQFHVTQQCGTEPPFQNEFWNNKR
                     SGIYVDVLSGEPLFSSKDKFDSGTGWPSFTKPLKEDRIIENADTSHNMRRVEVRSKDA
                     DSHLGHVFDDGPGPTGLRYCINSASLRFIPAEDLEREGYGEYLSLFQEDEARSGHMES
                     PGKDDPDYEMATFAAGCFWGVEALFQQVRGVLETKVGYTGGTAPNPSYAQVCTGTTGH
                     AEAVQIKYDPKVVSYSELLSIFWRMHDPTTPNRQGPDIGTQYRSAIFYHNEEQRIAAE
                     ESREEFDRSGAIARKAVTQIVPASEFFEA"
     gene            complement(2822..3064)
                     /locus_tag="PLV49_04685"
     CDS             complement(2822..3064)
                     /locus_tag="PLV49_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98175.1"
                     /translation="MGRSFESVRMGVNDLSLRWSKAGRALKKEDQSYAKELADMAKKH
                     SSEAFYALDDPLEAAVFSVLIELLKDREIYKQDPVK"
     gene            complement(3064..>3582)
                     /locus_tag="PLV49_04690"
     CDS             complement(3064..>3582)
                     /locus_tag="PLV49_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98176.1"
                     /translation="MEFELHPAIIIESRAFAILVAPLQMLIESLNDHLGIDRYQVLYI
                     CGNYSRVLSRLDRRFRDLEVRRAFTVFQLLTVLEESHHTLMILEHDPLLYEGAGEMVE
                     YASMAMREAAKGSILLLYSPTSDPYLEKMMTYADRVFCFAEGPCAAPRLSAKNNRKTQ
                     ASSTGQTTLESF"
BASE COUNT          922 a          853 c          790 g          917 t
ORIGIN      
        1 tttcatatca tcaccttctg gtaaaaggca tcttcagata tcaaaggagg ttatgagctc
       61 ttctaatatc acttaacctc ctcgaatttc ctgctatatc gatctgaaat gatgctacat
      121 ctacccttcg cttttgacaa tagaatctga gcttcgcttc aatgtctttt tgaggatgaa
      181 gatggattta taattggagc acggtggaaa ttgcgcgaat cctcagaaag aatattatca
      241 tttatggcgg gtgctatctg aagaccacac cgcctaaaag gagcaaaata gcccctaaaa
      301 agcctgtgat ggtgattatc gtctctgtac ttatatcacg gtgcacgagc gcccccttat
      361 gctgattcag gataatagct tttcgctcgg tctagccccc ggctttttgg gcggtatcaa
      421 gagcgcgtgg ctaccactgc aaaaggcaga atatccagca cggtcaccat cgcaactagt
      481 cctatccagt agaggggctt attaaaatac caggttctct ttccccgtgc atagacataa
      541 gcgaggtacg ggaaaagggg caaaattgag accaaaggat gaggctccac aaataggacg
      601 taggtgaaga tatatccttg caagagcagg cagacgacag ttgccagacc aaccttggct
      661 tgattgttga gtctcatatc tcttgccact cgctcttggt tgataagcct ttcagatgca
      721 ataatatgtg agtcaattac ttgtagaaat atgcataaaa tgtaagggga agacaaagtc
      781 ttctctgact gctggaggga tatacagacc atacccctcc agatggacat agatacgatg
      841 agccagagaa tctagaatgc aagaagcaat gtcgccaggt tgattgagcc cccaaataca
      901 agcgataata ttgccagaat gatgaatgcg atcacaagcc atttagctat atccatagat
      961 attcctgcgc cgcgccagcc gagcactccc aggatcaggg ctattgcaaa gaataccact
     1021 gccaaatata ataaatccac cattttatac actcccattc gtatctttgt ctgcattctc
     1081 atcgatgatg aagaatcctg accggcgtaa aatatatccg gatgaaggct tcatcgatca
     1141 agatgaaagc agcttaataa tacctatgac tctctttaaa tttaagactt ttggggctaa
     1201 tatttacata atacaaaata taatttataa caaatataat tctacttttg atgagatgtg
     1261 gacaatgaga ttaatctgtc ttattgctca taccatcata gctattttgg ccttgaagca
     1321 aaatccctga atcggatcaa ttctctggga tgatcgatga cgtctggctc aagcagacct
     1381 ttgcatctca tataatgagc tataaatcaa tataccataa agtatatatg tgcaagaacc
     1441 ggtttaaaaa acattatgcc aaagactact ctgacattat cagaaccaat agcaaaagcc
     1501 ctgaagatct atacagttca agccaaaagc agcatgcgcg ctcaaagcca ggtgatagaa
     1561 gaggcgctaa gagagttctt cgagaaccat ggaattaaag tcgaggagta gctacttttt
     1621 gcatctcctg ctctgcactc aacgttgttc tatcatgctc tcggttccgt caggctgacc
     1681 catataccag acccgctgat aaacagcatt tatagaattt gagatgattt tcacgcaaaa
     1741 cgcaaaaaac aggttaggga tcggggaatt gcaggctatg ctctcgcctt attttcttnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc cgcctcaaaa aattctgatg
     1921 caggaacgat ctgagtgacc gcttttcttg caatggcccc agatcgatca aactcttccc
     1981 gggattcctc tgctgctatt ctttgctcct cattatggta gaatattgca gagcggtatt
     2041 gagtgccgat atcaggaccc tggcggttgg gcgtggtggg atcatgcatc cgccagaata
     2101 tggagagcaa ctcgctgtag gaaacgacct tcggatcgta ctttatctgc acggcctcag
     2161 cgtggccagt agttcctgtg caaacctgtg cgtaggatgg attgggagca gtccctccgg
     2221 tatatcccac ttttgtttcc agcactccgc gaacctgctg aaagagagct tccacccccc
     2281 agaagcagcc tgcggcaaat gtggccatct catagtctgg atcgtccttc ccgggactct
     2341 ccatgtgccc agatcgagcc tcatcctcct ggaaaaggct gagatactct ccgtagccct
     2401 cccgttccag atcctctgcc ggtataaagc ggagggacgc agagttgatg caataacgca
     2461 atcccgtcgg cccaggacca tcatcgaaca catggcccag atgggaatct gcgtctttgc
     2521 tgcgaacctc aacccgcctc atattatggc tggtgtcggc gttctcaatg atgcgatcct
     2581 ccttcagggg ctttgtgaag ctcggccagc cggtaccgga gtcgaactta tctttcgagc
     2641 tgaacagggg ttctcctgag agcacatcca cgtagatgcc ggatcgttta ttattccaga
     2701 attcattttg aaagggaggt tcggtgccgc actgctgggt gacatgaaac tgcagcggcg
     2761 tcagtttctt tttggaattc gttttggaat ctttcaggga atcctctctt ctcataagca
     2821 cttattttac gggatcctgc ttatagattt ctctatcttt cagcagctcg atcagcactg
     2881 agaataccgc cgcctccaga ggatcgtcca gagcgtagaa tgcctcgctt gagtgctttt
     2941 ttgccatgtc ggcaagctct tttgcatagg actgatcctc cttctttaaa gctcgaccgg
     3001 ccttggacca gcgaagagat aggtcgttta cccccattct tacgctctca aagcttcggc
     3061 ccatcagaag ctctccaggg tggtctggcc agtggaactg gcttgcgttt ttcgattatt
     3121 ctttgcagaa agccgtggcg cagcacaagg cccctcggcg aagcagaata ctctgtcggc
     3181 ataagtcatc atcttctcta aataaggatc ggaggtgggg gaatagagaa gcaaaatgct
     3241 gcccttcgct gcctctctca tggccataga tgcatactcc accatctctc cagccccctc
     3301 gtaaagcagg ggatcatgct ccaggatcat caaggtgtgg tggctctcct ccaggacggt
     3361 tagcagctgg aagacggtaa atgccctcct tacctccaga tctctaaacc tgcgatccag
     3421 tctgctcagg acgcgagagt agtttccgca gatgtagagg acctgatacc tgtcgatgcc
     3481 cagatgatcg ttgagggact cgatcagcat ctgcaatgga gccactagta ttgcaaaggc
     3541 tctgctctcg atgatgatcg caggatgcag ttcgaattcc at
//
LOCUS       DAOSGP010000131         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_24213_length_3571_cov_15.107793, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000131 DAOSGP010000000
VERSION     DAOSGP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24213_length_3571_cov_15.107793"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLV49_04695"
     CDS             <1..386
                     /locus_tag="PLV49_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOG98177.1"
                     /translation="DAIEYARAHNKDVVLADTAGRLHTNINLMDQMRKIVRVTTPDLL
                     IFVDEAIAGNDAVERARLFNESVPIGGSILTKTDADAKGGSAISIAYITGKPVLFLGV
                     GQTYPDLVKFEPEWLVNRLMGEAEA"
     gene            complement(436..1113)
                     /locus_tag="PLV49_04700"
     CDS             complement(436..1113)
                     /locus_tag="PLV49_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG98178.1"
                     /translation="MIIKGSFICILVSAVILTQISIIAQSGFCAPASHDEEGISCYNK
                     GIDLAEQGLYDEAIAEFDRYILDNPDYARAWYNKGVALIQQGRHNEALIAFDRVTDIE
                     PQNSQAWYNRGVVLYILGRYDEALEASNRSTELNGEDARAWINEGNVLFQLNRHDQAV
                     AAYDRAIKIDPQSALAWCNRCNALINQSEYDLALSSAQKAIKIDPEMAEAYSCREAAL
                     MELGRDS"
     gene            complement(1185..1892)
                     /locus_tag="PLV49_04705"
     CDS             complement(1185..1892)
                     /locus_tag="PLV49_04705"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG98179.1"
                     /translation="MFAPVGTEEIIPRKSFPAVTAAIVAINVLFFLYEIFILFTEGSP
                     GVEAFFNTFGLVPADVLSGQSSMLPAYSTFFTSMFVHAGILHILSNMVFLAVFGDNVE
                     DLLGPVPYLIFYLLCGLAASAAQIAADPASTIPNVGASGAISGVLAGYLILLPTGTVR
                     LFIFFGFFYRITKIPALLFITVWFVIQLFSGVASLGVVAEGGGVAYWAHIGGFIAGLL
                     LAFAYKTIMRRHLFPSH"
     assembly_gap    2122..2221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2222..3046
                     /gene="htpX"
                     /locus_tag="PLV49_04710"
     CDS             <2222..3046
                     /gene="htpX"
                     /locus_tag="PLV49_04710"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOG98180.1"
                     /translation="NMIRTTLLLAGLTGILLLIGGYFGGRGGMLIALIFSILMNFGTY
                     WYSDKLVLKMYKARELTVAEAPELFGMVENLAANAGLPMPRLYVVDNPMPNAFATGRD
                     PKHAAVAVTTGITRILNRDELAGVISHELAHVRNRDTLISTIAATLAGVITFMANMAQ
                     WSLIFGGGDDDGANPLGVLAMAILAPLAATIVQLAISRGREFEADAGGAKISGKPLSL
                     ASALAKLESGNNAVRSDVNPSTAHMFIVNPLKGKAVASLFSTHPSTEERIARLRSM"
     gene            complement(3093..>3571)
                     /locus_tag="PLV49_04715"
     CDS             complement(3093..>3571)
                     /locus_tag="PLV49_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOG98181.1"
                     /translation="DSLDSTIIKTFRNSGYRATPQRIAISRYILRNHEHPSAQKTYQE
                     VKKTHPTVSLATIYTTIKILKKTGLICELKLPQSQTRYDPNTEPHAHLVCLQCGSVTD
                     WMDPIMQKLVKNVSKKANFTVIGSSFDLEGICALCGRRAKDALKSASPPAESISAE"
BASE COUNT          811 a          918 c          922 g          820 t
ORIGIN      
        1 tcgatgccat cgagtatgcc cgggcccata acaaggatgt agtcctggcg gacactgctg
       61 gcaggctgca taccaacatc aacctcatgg atcagatgag aaagatcgtt cgggtcacca
      121 cccccgatct gctgatattc gtggatgagg caatagcagg aaacgatgcg gtggagaggg
      181 cgcgactgtt caatgagtca gtgcccatcg gcggatcgat tctcaccaag acggatgccg
      241 acgccaaggg cggatctgcc atatccatcg cttatataac cggcaagcca gtgcttttct
      301 taggagtggg acagacctat cccgacctgg tgaagttcga gcctgaatgg ctggtgaata
      361 gattaatggg tgaggcagag gcataaattc tcgatgatac aagccaccac tgaccttgat
      421 cagggcggca gccgcctatg agtcccttcc cagctccata agtgccgctt ccctgcagct
      481 gtaagcctct gccatctctg gatcgatctt gatggccttc tgggcagacg ataaagccag
      541 atcgtactcc gactggttga tcagtgcatt gcagcggttg caccaggcca gggcagactg
      601 gggatcaatc ttgatggccc ggtcatatgc tgccaccgcc tgatcatgcc tattcaactg
      661 aaagagcaca tttccctcat tgatccaggc cctggcatct tcaccattca gctccgtgga
      721 cctgttggat gcctccagcg cctcatcgta tcttcccagg atgtacagga ctacgcccct
      781 gttataccag gcctgggaat tttgcggctc gatatcggtc accctgtcga aggcgatgag
      841 ggcctcattg tgcctgccct gctggattaa agcaaccccc ttgttgtacc aggcccgggc
      901 ataatccgga ttgtccagta tgtagcggtc gaactccgct atggcctcat catacaggcc
      961 ctgctcggcc aggtctatgc ccttattgta gcagctgatg ccttcctcat catgcgaggc
     1021 gggtgcacag aagcccgatt gagctattat tgatatctgc gttaagataa cggcagaaac
     1081 caggatacag ataaaactcc ctttgatgat cattcctccc caggatgtcc attctgcata
     1141 tctgaagaaa aagattttgg aggcccttat cgctctcctt tccctcagtg cgacgggaat
     1201 agatgccgtc tcatgattgt cttgtaggca aaagcgagca gtaatcctgc tatgaaacct
     1261 ccaatgtgag cccagtaggc cacaccaccg ccttctgcaa ccacccccaa cgaggccacg
     1321 ccgctaaata gctgtatgac gaaccagacg gtgatgaaga gcagcgctgg gatcttggtg
     1381 atgcggtaga agaatccgaa gaatataaac aggcgcacag tgcccgtggg caggaggata
     1441 aggtacccgg ccagaacccc agagattgcc ccgctggctc ccacattggg gatggttgaa
     1501 gcggggtcag cggcgatctg ggcggctgat gccgcaaggc cgcagagcag atagaagatc
     1561 agatagggta cggggcccaa gaggtcctcc acgttatcgc caaatacggc aagaaagacc
     1621 atgttggata ggatatgcaa tatgcccgca tggacgaaca tggaggtgaa gaatgttgaa
     1681 taagctggca gcatgctgct ctggccgctt aggacatcgg caggcaccag gccgaaggta
     1741 ttgaagaagg cctctactcc cggtgatccc tccgtgaaca gaatgaatat ctcatagagg
     1801 aaaaagagga cgttaattgc cactattgca gccgttaccg ccgggaaact cttgcgcggt
     1861 atgatctcct ctgtgcccac tggtgcgaac atatttcacc tcaaacttgg tcttctcttg
     1921 tactctatta ggcgcttgta tgtgctctct tcggctcttt tacttgctat aaaattacct
     1981 atcaatatac ttatccgatg catcgagagt ggacaactca ttcgatggta tccgatcagc
     2041 caatagcaaa agataaataa ctctcaagac gaaccaatac ctatggccaa tatgatcaga
     2101 acgaccctat tgctggccgg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 naatatgatc agaacgaccc tattgctggc cggtctgaca ggaattttgc tgctgatcgg
     2281 cggctacttt ggtggaagag gtggcatgct catagccctg atcttctcca ttttgatgaa
     2341 ctttggaact tattggtaca gcgacaaatt ggtcttgaag atgtataaag cccgcgagct
     2401 cacagtagcg gaggctccgg agctcttcgg catggtggag aatctggccg ccaacgccgg
     2461 ccttcccatg cccaggcttt atgtggtcga taatcccatg cccaatgcct ttgctacggg
     2521 cagggatccc aagcacgcag cagtcgccgt taccactggg atcaccagga tcctaaatag
     2581 agacgagctg gcaggagtga tctctcatga gctggctcat gtccggaatc gggacactct
     2641 gatcagcacc atagcggcta cccttgccgg agtgatcaca tttatggcca atatggccca
     2701 gtggtcgctg atcttcggcg gaggggacga tgatggtgcc aaccccctgg gagtcttagc
     2761 catggccatc ctggcccccc ttgccgccac cattgtccag ctggcgatat ccaggggcag
     2821 ggagtttgag gccgatgccg gtggagcgaa gataagcggc aagcccctct ccctggccag
     2881 tgccctggcc aagctggagt cgggcaataa tgccgtgcgt tcggacgtga atccatccac
     2941 ggctcatatg ttcatcgtca atcccctcaa aggaaaggca gtggcgtcac tcttctccac
     3001 ccaccccagc acagaggaga ggatcgcccg gctcagatcc atgtaaatca agtgattaag
     3061 actggtatcg acaaaaaggt ttataacgat ttttactcgg ccgatattga ttctgccggt
     3121 ggggaggcgc tttttagggc atcctttgcc cttcgaccgc acaaagcgca gatcccctcc
     3181 agatcaaagc tcgatccgat tacagtgaag ttcgccttct tggagacatt tttcaccaat
     3241 ttctgcatta tcgggtccat ccaatcggtg acgctaccgc actgaaggca gaccaggtgc
     3301 gcatggggct ctgtattggg gtcatacctg gtttgagatt gaggcagttt caactcacag
     3361 atcaggccgg tcttttttag aatttttatt gtggtgtaga tggtggccag gcttacggta
     3421 ggatgggtct tcttgacttc ctgatatgtc ttctgcgctg aaggatgctc atgattgcgc
     3481 agtatatatc tgctgatggc tatcctctgt ggagtggctc tatagccgct gttgcgaaat
     3541 gtctttatga tggttgagtc caggctgtct g
//
LOCUS       DAOSGP010000132         3550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_24401_length_3550_cov_19.959371, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000132 DAOSGP010000000
VERSION     DAOSGP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24401_length_3550_cov_19.959371"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLV49_04720"
     CDS             complement(<1..489)
                     /locus_tag="PLV49_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HOG98182.1"
                     /translation="MSGRTDQLRDLVLKIGATTKVVEKQATRHGSLRGSGEIEQALLG
                     VVREMIKQYNIQTKLTPEQLSSVVRLFYKGLSDTEIAEQLGDRALNKTVSRARIKLHL
                     FRETDLKPPFDKDEFIRLAEANRSVKDMAEALRVAPSTISEYRNIFDSQKASERDGYT
                     KRF"
     gene            591..1046
                     /locus_tag="PLV49_04725"
     CDS             591..1046
                     /locus_tag="PLV49_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HOG98183.1"
                     /translation="MSETSIYVVKTTANQERSVANMIAQIARKEKYDIRALLVPDVLK
                     GYVLVESPAPEIVDQAIQGIPHARSVIKGASNIGEVEHFLTPKPAVIGINEGAIVELI
                     SGPFKGEKARVKRVDLAKEEITVELFEAMVPIPITVRGDHVRVLSKDET"
     gene            1049..1534
                     /locus_tag="PLV49_04730"
     CDS             1049..1534
                     /locus_tag="PLV49_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOG98184.1"
                     /translation="MIYLANVVEALVPGGKASAGPPLGPALGPLGVNVAQVVAKINEE
                     TSDLNGMQVPVKVIVKSRTEFEIEVGTPPTSALIIKEIGGEKGTGDKTTIGDLTMEQV
                     LKIANIKRKGLLSKSLKNAAREVIGTAGSVGATVDGMSSKDAQLAVASGKYDEILEAK
                     A"
     gene            1619..2251
                     /locus_tag="PLV49_04735"
     CDS             1619..2251
                     /locus_tag="PLV49_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG98185.1"
                     /translation="MDIEEAVKQAITEAPARKFAESVDLAINLHNIDLSLPGNRVDAE
                     VILPHGLGKPTKIAVFAAGETALRAKSAGADRVISGDEIKEFAGDKRAARKLADEYKF
                     FIAETQFMPVIGKSLGSILGKRGKMPTPLPPTQDVTPQVQRLKNMTRIRSRDRPTFHL
                     TIGNRNMNTKELADNIEAVVVKLEQTLKDGRHNLKSVYVKTTMGPSIRVI"
     gene            2252..3226
                     /locus_tag="PLV49_04740"
     CDS             2252..3226
                     /locus_tag="PLV49_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOG98186.1"
                     /translation="MAGQIRHTTHIPEWKVKEVDELVDKISKSRVVGVVGLREIPADN
                     LQKMRGDLRGNVEIRMVRNTIARRALEASPAEIKPLADFIEDQTALIFSDLNPFKLNS
                     MLEKGKQPMPIKAGTRAPKDIVIEAGETSFSPGPMVGKLQAAGIPAAIKGGKVVINQK
                     ITLAKEGEVVPAKTAEILKTMEIFPRNVGLELRGAYEGGLIFRSKDLAIDVEGQVARI
                     SEASAKAFSFAVEIGYPTPATIGPMLQKAQTKARALVLDAGMIVPSMMEFILAKAAAN
                     ASAIAGLASGEMAQAAPAATAAPAAEEKQEEKKEEEGDTAAGLGALFG"
     gene            3257..>3550
                     /gene="rpl12p"
                     /locus_tag="PLV49_04745"
     CDS             3257..>3550
                     /gene="rpl12p"
                     /locus_tag="PLV49_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HOG98187.1"
                     /translation="MEYVYAALLLHSAGKDVTEEGIKNVVAASGVAADDVRIKALVAA
                     LEGVDITKVLSQAAAVPVAAAAAPVAAAAAAAAPAAKEEKEESKEAAEESGVEG"
BASE COUNT          927 a          856 c         1015 g          752 t
ORIGIN      
        1 aaacctttta gtgtagccat ctctctccga tgccttctgg ctgtcgaaga tattcctgta
       61 ttcggaaatc gttgaaggag ctacgcgaag ggcttcggcc atatccttga ccgatcggtt
      121 ggcctctgcg aggcgaataa actcgtcttt atcaaatggt ggtttcagat ctgtctccct
      181 gaagaggtga agctttattc tcgctcgact gacggtttta ttaagggctc tgtcgcccaa
      241 ctgctcggca atctcagtat cgctcaatcc tttatagaaa agccttacaa cagaggataa
      301 ctgttcagga gtcagtttcg tctggatgtt gtattgcttt atcatctccc ttactactcc
      361 caaaagggct tgttcaatct cgccgcttcc ccggaggctg ccatgccggg tagcctgctt
      421 ttccacgacc ttggtggtgg cgccaatctt gagtacgagg tcacgcaact gatcagtcct
      481 accactcatt ttagatactc acccctcaac aatcaccttg gctaatcgta taaagagatt
      541 tcgattgatt acaaagcttt ttataggcgg cccccgtcta aaagggcgga atgtccgaga
      601 ccagcatata tgttgtaaag accactgcga accaagaacg atcagtggcc aacatgatcg
      661 cccagatagc gcgcaaggaa aagtatgata taagggcact gctggttcca gatgtgctga
      721 aaggctacgt acttgtggaa tcaccagctc cagagattgt ggatcaggcg atccagggaa
      781 taccgcatgc gcgttctgtg atcaaagggg cctcaaatat tggagaggtt gagcactttc
      841 tcacccccaa gcctgccgtt atcggtatca atgaaggcgc aatcgttgag ctcatatccg
      901 ggccgttcaa gggggagaag gcaagggtca agcgcgtgga ccttgctaaa gaagagataa
      961 ctgtagagct cttcgaagcc atggttccca tccccataac agtgcgggga gatcacgttc
     1021 gcgttctcag caaagatgaa acttagaggt gatttatttg gctaatgttg tagaagcatt
     1081 ggtcccaggc ggaaaggcta gcgcgggacc acctttgggg ccggctcttg gacctctggg
     1141 cgtgaatgtc gctcaggtgg ttgccaagat caatgaagag acaagcgatc tcaatggaat
     1201 gcaggttcca gttaaggtca ttgtgaagtc cagaactgag tttgagatcg aggttggaac
     1261 gccgcccaca tcggcattga tcatcaagga gatcggtgga gaaaagggca ctggcgataa
     1321 gacaacaatc ggtgacctga caatggagca ggtcctgaag atcgcgaaca tcaagagaaa
     1381 gggccttcta tccaaatcgc tcaaaaatgc cgctcgcgag gtaataggta ctgcgggatc
     1441 ggtgggcgcc accgtggatg gcatgtccag caaggatgcc cagctggcag tggccagcgg
     1501 caaatacgat gagatattag aagcgaaggc ttaaatatag tgttatgatc tgagttttga
     1561 ttctcttttt tgagaagccg taggagacca taagagtcgc agactacgga gggttgaaat
     1621 ggatatcgaa gaagccgtaa aacaggctat cactgaggcc cctgccagga agtttgccga
     1681 gagcgtggat ctggcgataa acctccataa tatcgaccta tccctgcctg ggaatagagt
     1741 cgatgccgag gttatccttc cccacggact gggcaagcct accaagatcg ctgtattcgc
     1801 tgccggtgag actgccctgc gcgccaagag cgcgggagca gaccgggtaa tctccgggga
     1861 tgagatcaag gagttcgccg gagataagag agcagcgcgc aagctggcgg acgagtacaa
     1921 gttcttcatc gcagagactc agttcatgcc tgtcatagga aagagcctgg gttcaatcct
     1981 gggcaagagg ggcaagatgc ccacgccact gccgccgacg caggatgtca ccccgcaggt
     2041 gcagaggctt aagaacatga caaggatcag gtccagggac aggccgacct ttcacctgac
     2101 gattgggaac agaaatatga ataccaaaga gctggctgat aacattgaag cagtggtcgt
     2161 caagctcgag cagactctaa aggatggtcg gcataacctg aaatcagttt acgtcaagac
     2221 caccatgggc ccttccataa gggtgatcta aatggctgga cagatccgtc acactaccca
     2281 tataccggaa tggaaggtca aagaggtaga tgagctggtc gataagatca gcaaaagcag
     2341 agtggtgggt gttgtaggtc ttcgtgagat cccggcggac aatctgcaga agatgagagg
     2401 ggatctgaga ggcaatgtgg agataagaat ggtgaggaac accattgccc gccgtgccct
     2461 ggaggcttcc ccagctgaga taaaaccact ggccgacttc attgaggatc agacggcatt
     2521 gatcttcagc gatctgaatc ccttcaagct caatagcatg ctggagaagg gcaagcagcc
     2581 catgcccatc aaagctggca cccgtgcccc caaggatatt gtgatcgagg caggtgagac
     2641 ctctttctcg ccagggccca tggtcggcaa actgcaggct gcaggcatcc ccgcggcaat
     2701 taagggcgga aaggtggtca tcaatcagaa gatcaccctg gccaaagagg gtgaggtggt
     2761 ccctgccaag accgcagaga tccttaagac catggagatc ttccccagaa atgtaggtct
     2821 ggagctgaga ggcgcctatg agggcggcct gatattcaga tccaaggatc tagccataga
     2881 tgtggaaggc caggtcgcca ggattagcga ggcttctgct aaggcattca gctttgcggt
     2941 ggagatcgga taccccacgc cagctaccat tggacccatg cttcagaagg cccagaccaa
     3001 ggccagagcc ctggtgctgg atgcgggaat gatcgttccc agcatgatgg agttcatttt
     3061 ggccaaggcg gccgctaatg caagtgccat cgccggcctt gccagtggag agatggctca
     3121 ggctgcaccc gcagccacag cagcgcccgc tgcagaggaa aagcaagaag agaagaagga
     3181 agaagaagga gacaccgctg caggacttgg tgctctattc ggttaatttg gtctcaatta
     3241 atagaggttg atataaatgg aatacgtata tgctgcatta ctgcttcata gcgcaggcaa
     3301 agatgttact gaggaaggaa taaagaatgt cgtcgcagcc tctggtgttg ctgcggatga
     3361 tgtcagaatc aaggccttgg tggccgcttt agaaggcgtg gacattacca aggtcctctc
     3421 tcaggccgct gcagtccccg tagccgctgc cgcagcgcca gtcgctgctg ctgccgctgc
     3481 agctgcaccc gcagccaaag aggaaaaaga agagagcaaa gaggctgcag aggagagcgg
     3541 agtagagggc
//
LOCUS       DAOSGP010000133         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_24638_length_3528_cov_14.672041, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000133 DAOSGP010000000
VERSION     DAOSGP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24638_length_3528_cov_14.672041"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1994
                     /gene="cdhA"
                     /locus_tag="PLV49_04750"
     CDS             <1..1994
                     /gene="cdhA"
                     /locus_tag="PLV49_04750"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CO dehydrogenase/acetyl-CoA synthase complex
                     subunit alpha"
                     /protein_id="HOG98188.1"
                     /translation="TIMGSKPKTFKDFEEGLFYLEEQVVQLLAATHTGQEGAYIDFES
                     KALHAGRLDLLGMEICDMLQIVAYDMPRGAADAPIIEIGMGTMDQNKGVLIAYGHNLA
                     AGAEAMMYAENNNLWDKVDIGGVCCTAHDLNRIGTGTGELKVPKDIGKKPKVAGAMGW
                     WRKMIRSGVMDCVMVDEQCVFCDVLGDAEKRKIPVIATTDKIMLGLPDRTNDSVDAIV
                     DDLVNFKVPGVAVLDPVKAGEIGIRVAIAVKPKRAEAKIQNLITEEQFQEGIKKCTQC
                     NECAFVCPPHIRISKLIAEALKGNNQEFSNFYEICVGCGRCEQTCPQGINILDLFEYA
                     NRQTVKDQKFKMRSGRGPARDTEIRAVGAPIVLGTIPGIIALVGCSNYPNGTKECYDI
                     AKEFVDRGYIVVTSGCMAMDMSLYTDEDGKTIWEQYPGGLDGRNILNTGSCVSNANIG
                     DAAIKIATIFAHRNHRGNFDDIADYILNKVGACGVAWGAYSQKAASIATGCNALGIPV
                     VVQPHSVMYRRTFMGRTDKPEDWMVIDARDGSMQQIEPAPEALLYVAETKEEAMMEMA
                     KLCFRPSDNSIGRAIKLTHYCDISMKYCGKLPDDWHVYVRDTKDLPLTYKEQMMKELE
                     SKHGWQVDWKAKKFISGPLRPADVSFDPTNLARKIRTKK"
     gene            2015..2578
                     /gene="cdhB"
                     /locus_tag="PLV49_04755"
     CDS             2015..2578
                     /gene="cdhB"
                     /locus_tag="PLV49_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/acetyl-CoA synthase complex
                     subunit epsilon"
                     /protein_id="HOG98189.1"
                     /translation="MAGEVKKGIDTTKNAIPFEMAQIPGPEMAKTYMPKVMGAIIKKA
                     KRPLLVVGAELFDDPIMFDKMIEIGKMGIPIAATAHSVKGFVDRGYLDNVYQIGLHPL
                     TANLRFPDWKGLDGQGQYDVVIFMGIFYKFANAMFSTLKNFNRDIKRISIDRYYHVNA
                     NMTFGNMAFNPDDYHAAVDEVIAAMKK"
     gene            2619..>3528
                     /gene="cdhC"
                     /locus_tag="PLV49_04760"
     CDS             2619..>3528
                     /gene="cdhC"
                     /locus_tag="PLV49_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_function: GO:0043884 - CO-methylating acetyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HOG98190.1"
                     /translation="MADIKLDISPMYEGERIRKDDLWVELGGPKADGFELSLATPMDQ
                     IEDGKVTVIGPDLGETPEGTTMPFGMIFRVGGEKIEKDLESIIERRNHALLSYISGLM
                     HLNQRYDIWMRIGKGLKKKGVTSWKEIYGPVIDLYKAEMPFIEKMDITIVTDPAKVKE
                     ELAKAMAVYKQRDERAKGLHDEDVDVFYGCTLCQAFAPTSACVVTPDRPSLCGAITWF
                     DGRAAAKVDPEGPQFPIEKGSAVDEVSGEYAAINEMAEKRSGGEYSRMLLYTFFDAPH
                     TSCGCFETIGFYMPEVDGIGLADRDFK"
BASE COUNT          910 a          925 c         1005 g          688 t
ORIGIN      
        1 agaccatcat gggatccaag cccaagacct tcaaagattt cgaggaaggg ctcttttatc
       61 ttgaggagca ggtcgtacag ctccttgcgg ctactcatac cggtcaggag ggcgcttaca
      121 tagacttcga gtccaaggct cttcatgccg gcaggctgga cctactgggc atggagatct
      181 gcgatatgtt gcagatcgtg gcctatgaca tgccccgcgg cgctgccgat gcccccatca
      241 ttgagatcgg catgggcact atggatcaga ataagggcgt tctcatcgcc tacggccaca
      301 acctggcagc cggcgccgag gccatgatgt atgctgagaa caataacctc tgggacaagg
      361 tggatatcgg cggcgtctgc tgcacagcac acgatctgaa caggatcggc actggaacgg
      421 gcgagctgaa ggtgcccaag gatataggaa agaagcccaa ggtggctgga gcgatgggct
      481 ggtggagaaa gatgatccgc tccggcgtca tggactgcgt catggtggac gagcagtgcg
      541 tcttctgcga tgttctcggc gatgctgaga agcgcaagat cccggtcatt gccaccaccg
      601 acaagatcat gctcgggctt cccgacagga ccaatgattc tgttgatgcc atagttgacg
      661 acttggtgaa cttcaaggtt ccaggcgttg cagtccttga tccggtaaag gcaggagaga
      721 tcggcatcag ggtagccatt gctgtcaaac ccaagagggc tgaggccaag atccagaacc
      781 tgatcacaga agagcagttc caggaaggca tcaagaaatg tacccagtgc aatgagtgtg
      841 ccttcgtctg cccgccacat atcagaatca gcaagctcat cgctgaagca ctcaagggca
      901 acaaccagga gttctccaac ttctacgaga tctgtgtcgg ctgcggaaga tgcgagcaga
      961 cttgcccgca aggcatcaat atcctggacc tgttcgagta tgccaaccgc cagacagtca
     1021 aggatcagaa gttcaagatg aggtccggac ggggacctgc ccgcgacacc gagatcagag
     1081 ctgttggcgc accgatcgtg ctgggcacta ttcctggaat catcgccctg gtaggatgct
     1141 ccaactatcc caacggcacc aaggagtgct acgacatcgc caaggagttc gtcgatcgcg
     1201 gctacatcgt cgtcacctcc ggatgcatgg ccatggatat gtccctttac accgatgaag
     1261 acggcaagac catctgggag caatatcccg gcggcctcga tggcaggaac atcctaaaca
     1321 ctggctcctg tgtatccaac gccaacatcg gcgatgcagc catcaagatc gccactatat
     1381 tcgcccacag aaaccacagg ggcaacttcg acgatatcgc tgactatatc ctgaacaagg
     1441 taggtgcctg cggcgtggcc tggggagcat actcccagaa ggcagcatca atcgccacgg
     1501 gctgcaacgc actgggcatc cctgtagtcg ttcagcctca ctccgtcatg tatcgacgta
     1561 cattcatggg caggacggac aagcccgagg actggatggt catagatgcc agagacggct
     1621 caatgcagca gatagagccc gcacctgaag ctttgcttta cgttgcagag accaaggaag
     1681 aggcaatgat ggaaatggcc aagctctgct tcagaccgtc ggacaacagc atcggcaggg
     1741 caatcaagct gacccattac tgcgatatct ccatgaagta ctgcggaaag ctgcccgatg
     1801 actggcatgt ctatgtccgc gacaccaagg accttcccct gacctacaag gagcagatga
     1861 tgaaggagct ggagtcgaag cacggctggc aggttgactg gaaggccaag aagttcatct
     1921 ccgggcccct gagaccggcg gatgtgagct ttgatccgac caacctggca aggaagatca
     1981 ggaccaagaa gtgagggaga aggaggatat aaaaatggca ggagaagtta agaagggaat
     2041 tgataccacc aaaaatgcaa ttccctttga gatggcccag attcccgggc cagagatggc
     2101 caagacctac atgcccaagg tcatgggcgc tataatcaag aaggcgaaga ggccactcct
     2161 tgtagtgggg gcagagctct tcgatgaccc cataatgttc gataagatga tcgagatcgg
     2221 caagatgggc ataccgattg ccgccacagc tcattccgtc aagggctttg tggatagagg
     2281 ctacctggat aacgtttatc agatcggcct gcatccactg accgcaaatc tgcgcttccc
     2341 cgactggaag ggcctggatg gacaggggca gtatgatgtg gtcatattca tgggaatctt
     2401 ctacaagttc gccaatgcga tgttctcaac gctgaagaac ttcaaccggg acatcaagcg
     2461 catatccatt gaccgctact atcatgtcaa tgccaatatg acctttggca acatggcctt
     2521 caatccggac gattatcacg cagccgtaga cgaagtaatc gcagcgatga agaaatagag
     2581 tttatcctat aacagaagaa aacagaagag gtgtattaat ggcagatata aagttggaca
     2641 tatcccccat gtatgagggc gagagaatca gaaaggacga tctatgggta gagcttggtg
     2701 gccccaaggc agatggattc gagctgtcgc ttgccacgcc catggaccag attgaggatg
     2761 gaaaggtaac tgtcatcggt ccggatctgg gagagacccc cgagggcacc accatgccct
     2821 ttggcatgat cttcagagtt ggcggcgaga agatcgagaa ggacctggag tccatcatcg
     2881 agaggcgcaa ccatgccctt ctctcctaca taagtggcct catgcacctc aaccagagat
     2941 atgatatctg gatgaggatc ggcaagggtc tcaagaagaa gggagtcacc tcctggaagg
     3001 agatctacgg cccggtaatt gatctataca aggcagagat gccattcatt gagaagatgg
     3061 acatcaccat cgtcaccgat ccagcaaagg tcaaggaaga gctggccaag gccatggccg
     3121 tatacaagca gcgtgacgag agggccaagg gactgcacga tgaggacgtg gatgtattct
     3181 atggctgcac tctctgccag gcattcgctc ccaccagcgc ctgcgtggtc accccggaca
     3241 ggccgtcctt gtgcggagcc atcacctggt tcgacggcag ggctgccgcc aaggtggacc
     3301 cagagggccc ccagttcccc atagagaagg gatcggctgt ggatgaggtt agcggcgagt
     3361 atgccgccat caatgagatg gctgagaaga ggtccggcgg cgagtacagc agaatgctcc
     3421 tctacacatt cttcgatgca ccccacacct cctgtggctg cttcgagacc attggattct
     3481 atatgcctga ggttgacggc atcggccttg ccgatcgtga cttcaagg
//
LOCUS       DAOSGP010000134         3519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_24728_length_3519_cov_17.278868, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000134 DAOSGP010000000
VERSION     DAOSGP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24728_length_3519_cov_17.278868"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLV49_04765"
     CDS             <1..759
                     /locus_tag="PLV49_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOG98191.1"
                     /translation="PPAESLARENIQVIQDLGDKLNMHLSLKDLLQENLNQILHLRLP
                     VGIAAGAGLLWSATGVFVILSKNIDRAWSNSEGRNPLEWRLVGIAMVGSLLIGLIILS
                     MVVVSLFGLLDYGLGLLLGEGSAYHMPLESKLLYRLFPDAVIFIAFTILYWWAPKVDV
                     RWSDAALGAFVAALCWELSKMGFAWYLESGLSNHMLVYGSLGTVVILMLWIYLSGLIC
                     LFGAHLVAQMVGRRQAAAALRESHINKDLKADGA"
     gene            878..>1691
                     /locus_tag="PLV49_04770"
     CDS             878..>1691
                     /locus_tag="PLV49_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG98192.1"
                     /translation="MYVTKAFIAPLLLSFFFAFVLLPVYSRIHCFTGRKNLSAFLSLL
                     LVVLFFMIFLSSALNAIATETSGLFSSQEDIVQSIDIYITQKTEESFDTLHVVLNNIV
                     SESTADETVERLREMVRPFIEAPRDRILSDLLPQIAFEATNLATGFISELPVLMSQFF
                     VAIMITYYLLIGGSSAVKGFLLILPERELMNYFMGELNKIYNNLFNVYLLTSLITGLL
                     AAIGFALFGVSYPFLWGLTTAIFALLPMIGPPIIFVPICIYFLLIGNYLSGIG"
     assembly_gap    1692..1791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1792..2015
                     /locus_tag="PLV49_04775"
     CDS             <1792..2015
                     /locus_tag="PLV49_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98193.1"
                     /translation="VVGFLGFIIGPLLYGFLLAAFRTRIYMLKEEGAIDEKELAELSE
                     GEGLPPGPRFCGLGSKARSALEWLRRYRT"
     gene            2085..3155
                     /locus_tag="PLV49_04780"
     CDS             2085..3155
                     /locus_tag="PLV49_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG98194.1"
                     /translation="MENDLSCLWSHRNWLFAITLLLILLSSYFLWPLQDGIVLGIVFT
                     YLGRPVRDLFGKRRQIGSLAAIICIIAPLSIIFAAGVFEAVSQLRWLQSHRVQIVAAG
                     QEFISSIYIPQFIFDELSRGMDNLMGIGLSMLTSLPVFDLGTTLTLGLLNLLIAFCVC
                     YYLLVDGDRLLGATIDFLRQKKGDFELRCLGRIDSILSGIYTGSISTAMVYAIASVPI
                     FYLFDIPRPLAMASIILLAGVVPFLTWLVFLLTAISRYIEMGPMEAAVFFFAASVLVA
                     AVELVVRPYIVYARSSIHPMLVLLAFLGGGLVGGVFGFFLAPAMLGTISGIYQMIKSE
                     LTGIKRGHQVEKVDAFAAIQNN"
BASE COUNT          746 a          825 c          857 g          991 t
ORIGIN      
        1 cccccggcag agagcctggc aagagagaac attcaggtga tccaggatct gggggataag
       61 ctgaatatgc atctgtccct caaggatcta ttgcaggaaa atctcaatca gatccttcac
      121 ttgaggctgc cggtggggat tgcggcgggg gcaggtcttt tgtggtcggc gacaggcgtg
      181 tttgtgattc tgtccaagaa catagatcgc gcatggagca actccgaagg gagaaatccc
      241 ctggaatggc gtttagtggg cattgccatg gtcgggagcc tccttatcgg cctgatcatc
      301 ctctccatgg tggtagtctc cctcttcggc ctcctggact atggactggg tctgttgctg
      361 ggcgaaggaa gtgcttatca tatgccattg gagtcaaagc tcttgtacag actgtttccc
      421 gatgctgtga tcttcatcgc ctttaccatc ctctactggt gggctccaaa ggtggatgta
      481 agatggtctg acgccgctct tggggcattt gtggccgctt tatgctggga gctctccaag
      541 atgggctttg cctggtatct ggagagcggc ctttccaatc atatgctggt ctacggctct
      601 ttgggaacag tcgtcatcct gatgctgtgg atctatctga gcggtttgat ctgcctcttt
      661 ggagcgcacc ttgtcgccca gatggtggga cggaggcagg cggcagcagc acttagagag
      721 agccatataa ataaagactt gaaggctgat ggagcataaa gatgccccgt caaaaaatta
      781 tatattttag aatagctaaa ggatagctta catgccgggg tcaaagagat tcaacttatt
      841 agcatccact gtacttctca ttgtctttat cagcacagtg tatgtcacaa aggcattcat
      901 tgctcctctg cttctgagct tttttttcgc tttcgtcctg cttccggtct actctcgcat
      961 tcactgcttc actggaagga agaatttatc ggcgttccta tctttgctcc tggtcgtctt
     1021 gtttttcatg atctttcttt ccagtgccct caacgccatt gccacagaga catcgggcct
     1081 gttttcctct caggaggata tagttcaatc cattgacatt tatatcaccc agaagactga
     1141 agaatccttt gataccctgc atgtcgtctt gaataatatc gtctcagaat caaccgcaga
     1201 tgagactgta gagcgattga gggagatggt gaggcctttc atcgaggccc ccagagacag
     1261 gatcctctcc gatctgctgc cacagattgc atttgaagcc accaatttag ccacaggctt
     1321 catcagcgag ctgcctgtgc ttatgtccca gttctttgtg gccataatga tcacctatta
     1381 ccttctcatc ggaggttcca gcgcggtaaa gggattcttg cttatccttc ctgagaggga
     1441 attgatgaat tattttatgg gggagctgaa taaaatttat aataatcttt tcaatgttta
     1501 cctattgacc tcccttataa ccggactgct tgctgcaatt ggatttgccc tttttggggt
     1561 ttcatatccc ttcctatggg gcctgacaac ggccatattt gccctgttgc ccatgatcgg
     1621 accgccgatc atctttgtgc ccatatgcat ctatttcctc ctgatcggga attacttgtc
     1681 gggcataggt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttgtggtgg
     1801 gatttcttgg attcataata gggccgctcc tttacggatt tcttctagcg gcattcagga
     1861 ccagaatcta tatgctgaag gaggaggggg caatagacga aaaggagctt gccgagctct
     1921 cagagggaga aggcctgcct cctggaccca gattttgtgg tctgggctca aaggccagat
     1981 cggctctgga atggctgaga cgctaccgta cttgaggcaa ctggctgggc gagtcctggt
     2041 catcccaaag ttcatatcgt tgcagctctt attgagaatc agagatggag aatgatctgt
     2101 catgtctatg gagccataga aattggctct tcgccataac attgcttctc atacttctct
     2161 cctcttactt cctctggccg ctgcaggacg gcattgtctt gggaatagtc ttcacttatc
     2221 taggccgtcc agtgagagat ctgtttggaa agagaagaca gattggctct ctggcggcca
     2281 ttatctgcat catcgcccca ttatccatta tattcgctgc cggggtcttt gaggcagtat
     2341 ctcagctcag atggctgcag agccatcggg tgcagatcgt agctgccggc caggagttca
     2401 tatccagcat ttatattccg caattcatct tcgatgagct ttccagaggc atggacaatc
     2461 tgatgggaat aggactatcg atgctcacca gcttgccggt attcgatctg ggaacgacgc
     2521 tgaccctcgg gcttctgaat ctcttaatag ccttttgtgt atgctactac ctgctggtgg
     2581 atggcgatag gctcctcggg gcaacgatag acttcttgag gcagaagaag ggggattttg
     2641 agcttcgatg cctgggcaga attgatagca tcttaagcgg catctatacg ggcagcatct
     2701 ccaccgccat ggtctatgcg atcgcctccg ttcccatatt ctatctcttc gacattccca
     2761 ggcccctggc tatggccagc ataatactcc tggcaggggt ggtgccgttt ttaacctggc
     2821 ttgtcttcct cttgaccgcc atatccagat atattgagat ggggccgatg gaggcagcag
     2881 tcttcttctt cgcggcctcg gttctggtgg cagcagtaga gctggtcgtc cggccctata
     2941 tcgtctatgc cagatcgtcg atccatccca tgctggtgct gctcgcattt ctgggcggag
     3001 ggcttgtggg cggcgtcttt ggattctttc tcgctcctgc aatgctggga acgatctctg
     3061 gcatctatca gatgataaaa tcggaactga cagggattaa gagagggcat caggtggaga
     3121 aagtggatgc ttttgcggcc attcaaaata attaataaaa ctctaagaat gtaatgaatt
     3181 ccgtgcatta ttaatttttg tgccaaagat attttcatct atatattttg gtctcgttaa
     3241 tgaacgaata ccgaaaagta tatattcgtt gatcgcagta tagcgtattg gcaaaagtca
     3301 gaattctttt tccctcctac atcacagact tttgcctccc tccttattgc cctttcactg
     3361 ccttgtgacg ggccgattgc ctgaagctga tcgactgccc cgatcatcca ttattttttg
     3421 gtcgcaaaaa aaaccaagac ggcaatccat atatgccttg ccgttttcaa cttggcctga
     3481 atgttgagcg acttgcgcct ctccattgtg gcagagctg
//
LOCUS       DAOSGP010000135         3506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_24861_length_3506_cov_18.184584, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000135 DAOSGP010000000
VERSION     DAOSGP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24861_length_3506_cov_18.184584"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1096
                     /locus_tag="PLV49_04785"
     CDS             311..1096
                     /locus_tag="PLV49_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98195.1"
                     /translation="MNRRGIIHMLLAAVIAVCGQCQATEIPASQDVYVSLGTGNETVY
                     NQSDTLLCATNVPDANNTTLNSYPGVPLIQFNLSGIDIADDDIAILVLKAASVRQMDR
                     SGIVALVTIGSDWDEQSDITTFLVNILPARNLIVKNDLTLMSTNSDGDDIYAFDVSQK
                     LMQAKERGDKVSFLLEAIGNSTTEIDFLSKESGQGPYLMIMPYPGQPISGNADQAIPA
                     GQPVQADQANSDGISGQMNQTDQILSPDSVSAEPPVHAFGLVQ"
     gene            1275..2258
                     /locus_tag="PLV49_04790"
     CDS             1275..2258
                     /locus_tag="PLV49_04790"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOG98196.1"
                     /translation="MDFFSHALLPYLLGRFLRIRRDYLAALVLGGIAPDLDMLVVWIN
                     QIHPTSYLIVHRGFTHTFLFGFFVAAILLFLVSRDWIKTRSRGIVTEDLQFSPTTLAY
                     SYAGIISHLFLDFLTSGGVPLFYPLTAARYSAEIFSRLEIVIMIATLLFMAKLFRDRP
                     EMKIDKKVSAAFLIFLLVISGIRLEGKEMANGFHDDGFKIYPDSNLFQWTMLKNDSNN
                     NSFLIFKFNIFAGLLPDNASYLHQNAPLHKDGLDEALNRAESLPQVKLFRWRSSAVAI
                     NASEKEGNWIIEYYDPVIVTQYMNSWRPPNISSRGFGSIQVVANETGAELL"
     gene            complement(2331..3503)
                     /locus_tag="PLV49_04795"
     CDS             complement(2331..3503)
                     /locus_tag="PLV49_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOG98197.1"
                     /translation="MDYVLHPKSEIDSRIKKLKDRMDDIDGALLFQAVDMCYFSGTAQ
                     DGLVYIPRDGEPIVMMKRSLVRAKEESPLEVRPLKNMRNLKEDLGIKTSATIGLEMDV
                     LPCSFYFRVNKALEDASFVDVAERIKHIRSVKSEFEIKLAKEAAQMLVEGFSTVPDHI
                     KEGMTEVELMCRMESEMRLMGHQGSLRFRRFNSIVPIGHIMSGSSAAIPSFLASPTGG
                     RGTSVVFPHGPGYRKIKRNEPVFVDTVGICNGYIADATRIFSLGKIEPGLVEAYEASC
                     QIEEAIAREMMPGRTARELFELSESEGARLGYGDFLGGPVGSKCGFVGHGVGLELDEY
                     PVLAPLDHEIQEGMTIAVEPKIIYPGKGVLGVEDTFLTTSSGALRLTEMPLEIWEV"
BASE COUNT          840 a          983 c          839 g          844 t
ORIGIN      
        1 cgagaagata gaaaagtacg ctgccctggg cgagaagact cctcggagcc caatccctgt
       61 acgcgcatga agccatccgc ctggcagatg agactgaagg gcaagatgac ctgccagtag
      121 gaaagcataa gcatccaccc agcctcaggc cctatggtta tcccgcgcgc atttccgccc
      181 aagaagtgct tcctgtggct ctacattttt ctttctcttc acaaaaaaga tttccaaact
      241 ataattttaa atatgaatcg attttatgta atatcattct ggataagaat tgattgagga
      301 gagatgagat atgaatagaa gggggataat tcacatgctg ctggcggcgg tcatcgccgt
      361 ctgcgggcaa tgccaggcga cagagattcc cgcttcccag gacgtttatg tcagcctggg
      421 aacaggaaat gagacggtat acaaccagag cgatacactg ctttgcgcca ccaatgtgcc
      481 ggatgcaaac aataccactt tgaacagcta tcccggggtt ccattaatac aattcaacct
      541 ttccggcata gacattgccg atgacgacat cgcaattctg gtgctcaaag cagcatcggt
      601 gcgccagatg gatcgctcag gcattgtggc tctggtgacc atcggctcgg attgggatga
      661 gcaatccgat atcaccacct tcctggtcaa catcctgcct gccaggaacc tgatagtgaa
      721 aaatgacctc actctgatga gcaccaatag cgatggtgat gatatctatg ccttcgatgt
      781 ctcccaaaag ctcatgcagg caaaggaaag aggggacaag gtatcattcc tcctggaggc
      841 tattggcaac agcacgacgg agatcgactt tctgtctaaa gagagcggcc aggggccata
      901 tctgatgatc atgccctatc ctgggcagcc catctccggc aatgcagatc aggccatccc
      961 tgctggtcag ccagtccagg ctgatcaagc aaattcggac ggtatctcgg ggcagatgaa
     1021 tcagacagat caaatcctat ccccggactc tgtctcagca gagccacctg ttcatgcatt
     1081 cggactggta caataaagat caaggccaag ggggaagcca aggtgcctgg ggcgcagaac
     1141 cataggtaaa tcaggagcct tcccagatgc ctgcgaccat ctgacagttg atctcatcta
     1201 tactttttaa ttttgttctg gacaaaggcc gtttccaaaa agcaaaaata gccataatga
     1261 gctaaactgc tttgatggac ttcttctccc atgcattgct tccctatctc ttgggccgct
     1321 tcttgaggat caggagagat tatcttgccg ccctggtgct cggcggaata gctcccgacc
     1381 tggacatgct ggtggtctgg atcaatcaga tccatccgac ctcttatctc atagtgcaca
     1441 ggggtttcac ccacactttc ctctttggct tctttgtggc agcaatcctc ctctttctgg
     1501 tctcacgtga ctggatcaag accaggtcca ggggtatcgt tactgaagac ctgcagttct
     1561 cgcccacgac cctggcctat tcctatgccg gaataatcag ccacctcttc ttagactttc
     1621 tcacctcagg aggcgttccc ctcttctatc ctctgaccgc tgccaggtac tcagccgaga
     1681 tcttcagccg tttagagatc gtgataatga tcgccaccct cctatttatg gcgaagctct
     1741 tccgggaccg gccagagatg aaaatcgata aaaaggtgtc cgccgccttt ctcatatttc
     1801 ttttggtcat aagcggaata aggctggagg gcaaagagat ggcgaatggc tttcatgatg
     1861 acggcttcaa gatctatccg gactcaaatc tcttccagtg gaccatgctg aaaaacgata
     1921 gcaataacaa tagtttcctg atctttaagt tcaatatctt tgcgggattg ctgccggata
     1981 atgcatccta tcttcaccaa aatgccccgc tccataaaga cggattggac gaagccctca
     2041 atagagctga gagcctccct caggtcaagc tcttccggtg gagatccagt gcagtagcga
     2101 tcaacgcctc tgaaaaagaa gggaattgga tcatagagta ctacgatccg gtgatcgtga
     2161 ctcagtacat gaacagctgg cgcccgccga atatcagctc cagaggtttt ggatccatcc
     2221 aggtcgtagc aaatgagact ggggcggagc tcctctagcg aagttgtcca gacgaagcgt
     2281 cacaaaatcc aaaaacgaat ctgtacattc tgactccatc cgacaaggcc tcagacctcc
     2341 cagatctcca ggggcatctc cgtcagcctc agggctccag atgatgtcgt caggaaggtg
     2401 tcctccaccc ctaagacacc tttgcccgga tatatgatct tgggctccac ggcgatggtc
     2461 atcccttcct ggatctcgtg gtccagcggg gctagcactg gatactcatc cagctcaagg
     2521 cctactccgt gacccacaaa gccgcatttg ctccctaccg gtccacccag gaaatcgccg
     2581 tagcccagac gcgccccctc tgattcggag agctcgaaca gctctctagc cgtcctgccc
     2641 ggcatcatct ccctggcgat ggcctcttcg atctggcagg aggcctcata agcctccacg
     2701 agccccggct cgatcttgcc caaagagaag atcctggtgg catcggcgat atatccattg
     2761 cagatgccca cagtgtccac aaataccggt tcgtttctct ttatctttct gtagcctggc
     2821 ccatggggaa acaccaccga ggttcccctg ccccctgtgg gtgaggccag gaagctgggg
     2881 atggcagcgc ttgatccgga cattatatgg cctatgggaa cgatgctgtt gaagcggcga
     2941 aagcgcaggg atccctgatg gcccatcagc ctcatctccg attccatgcg gcacatcagc
     3001 tccacctcag tcattccctc tttaatatga tccggaacgg tggagaatcc ctccaccagc
     3061 atctgtgctg cttccttggc cagttttatc tcgaactctg acttgactga gcggatatgc
     3121 ttgatcctct cggcaacatc gacaaaagaa gcatcctcca gggccttgtt caccctaaag
     3181 tagaagctgc agggaaggac atccatctcc aggccaatgg tagcactcgt tttgatgccc
     3241 aggtcctctt ttagatttct catattcttc agcggccgga cctccagggg cgactcctct
     3301 tttgccctca ccaggcttct tttcatcatt acaatcggct cgccgtctct tgggatgtag
     3361 accagtccgt cctgggcggt tccagagaag tagcacatgt ccaccgcctg aaatagaagg
     3421 gctccgtcga tgtcgtccat ccggtctttt agctttttta tcctgctatc gatctctgac
     3481 ttggggtgaa gaacataatc catttt
//
LOCUS       DAOSGP010000136         3498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_24924_length_3498_cov_18.295382, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000136 DAOSGP010000000
VERSION     DAOSGP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24924_length_3498_cov_18.295382"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..87)
                     /locus_tag="PLV49_04800"
     tRNA            complement(4..87)
                     /locus_tag="PLV49_04800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Leu,seq:taa)
     gene            complement(314..907)
                     /locus_tag="PLV49_04805"
     CDS             complement(314..907)
                     /locus_tag="PLV49_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98198.1"
                     /translation="MRTVLAFALVLMLSSLAISAPESSQLGPYAVTFDMNTEMQYEMI
                     PLEAGESDAAAFYGLQVVTDNSTWARVVITEYKELIDSTIAPQKTITVLNAAVNGFNV
                     TSVEDTVIDGKEGYVASGVPFPGITSIPADTQLFEAVYWLDSEECECGPVSVGTTSVA
                     ISSTYPEDVTMNLINSLKIVKGEAAAAVAGEQVLPPE"
     gene            1028..1483
                     /locus_tag="PLV49_04810"
     CDS             1028..1483
                     /locus_tag="PLV49_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOG98199.1"
                     /translation="MRYGCDELGIYTIILSLEKAKQICIGALGEIDFASGCYAYTGSA
                     RGPGGCKRVDRHIEILQGSRITRRWHIDYLLAQAGLVEVFITRTSRDLECSIAEMIGE
                     RLVPIKGFGSSDCRCISHLHYGRDLVSTMAAVSLAHSRAASMTLEPSRE"
     assembly_gap    1853..1952
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1953..>3498
                     /locus_tag="PLV49_04815"
     CDS             <1953..>3498
                     /locus_tag="PLV49_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98200.1"
                     /translation="VNSSPNLSQVHLENNGITKLHRIKVTDRDRSLGTLSELDPGEKK
                     VLSISGRAEKLSVFALDPTGLEIQGRVKYNSTKQEKNASEDVGLETIFDPLLFSMSIP
                     AGGAIPAEAEVPIAEEPVIPAIPQNSSSSSLALNISVNRSACLAGEAISYRCLATNIG
                     QDELSDVRITCAGKLASTGFITPHKELELDGILLVENNTRLLAEASAKDGKGNIYTNN
                     TSIDIRMISPQINLLLEAPELVHRGENASILIRIENSGEENLTDLKVDDGFGEVTRIP
                     ALAAGEAEIVQQNRTILHSLPYEVIVSARDKVGGEVYSSQSQTIAVMNSSLEIQSEST
                     QVATYPGEPAEVTWILENTGQETLFNVTLQGNGADCILPQMLPGRSVRMAAIYSKNDS
                     TWINVTARGMDGRGFETTANGSVLLRAIKPGISLKVMPEKIEAAPGDEAEISCLVTNS
                     GEDRLREVVLTLDGAKLSSLGDLEPGEFRVVQARPLIEENSTLQFAVAGEDSQGEVWS
                     DRMAVEA"
BASE COUNT          883 a          867 c          892 g          756 t
ORIGIN      
        1 taatgcgggg tacgggattc gaacccgcga actcctgcga gacaggatct taagtcctgc
       61 gcctttggcc agcttggcga accccgcaac catataaggc cgccgcttac aataggacat
      121 gatggataaa tagtttcccg aatgatctca gaaaaaggaa tattatctgt ttagaggcaa
      181 aggggaggcc gtgtgagcct acctttgccg atatagcatc aggtatcggc aatgagccaa
      241 tggcccaaag cctattccag tgcttttact ctctatttgc catatcttga tagtatttat
      301 ctatccgtat tgcttattcc ggaggcagaa cctgctcgcc cgctactgct gctgccgcct
      361 cgcccttgac tatcttcagg ctgttgatca ggttcatggt cacatcctct ggataggttg
      421 aggatatagc tacacttgtc gtgcctacag agactggtcc gcactcgcac tcctcgctgt
      481 ccagccagta cacggcctca aagagctggg tatcggctgg aatgcttgtg atccccggga
      541 agggaactcc gctggctaca tatccttcct ttccatctat gaccgtgtct tctacactgg
      601 taacattgaa gccgttaacg gcggcattca ggacagttat ggtcttttgg ggagcgatgg
      661 ttgaatctat aagctccttg tactcggtta tgaccaccct tgcccaggtg gagttgtctg
      721 ttactacctg caggccgtaa aatgcagctg catcagactc tccggcctcc agcggtatca
      781 tctcatactg catttcggta ttcatatcga aagtgacggc atacggtcca agctgactgc
      841 tctccggcgc agatatagcc agagaggaga gcatcagcac caatgcaaat gctaatactg
      901 ttctcacaca attcaccgag cttctctttg ctcctcatcc tagaaaagtc ttgctttgat
      961 ataaatctcg tatcatccct ccaaaagagc attagtatca aatagcgata atgggaaacg
     1021 gattcatatg agatatggct gtgatgagtt gggaatctac accatcatcc tctctttgga
     1081 aaaggcgaag cagatctgta taggggccct gggtgagatc gattttgcct ccggatgcta
     1141 tgcttatacc ggctctgccc gggggcctgg cggctgcaag agggtggatc gccatataga
     1201 gatattgcag ggcagtagaa tcaccaggag atggcatata gattatctcc tcgctcaggc
     1261 gggcctggtc gaggtgttca taaccaggac atcgcgagat ctggaatgca gtatagcgga
     1321 aatgatagga gagcgtcttg tgcccatcaa gggatttggc tctagcgatt gccgctgcat
     1381 cagccacctt cactatggca gggatctggt gagcacgatg gctgcagtat cactcgctca
     1441 ttctcgggcg gcatcaatga cgcttgagcc atcgcgagaa taatcaaaat tataaactaa
     1501 ataaaatgaa aaaatagtta tagaagcggt ataagttcag ctgatagtga tatatctcca
     1561 ctgacataaa tctaaaagta tgcatgcatt cccctttttt gcccaaacat ccgaaaccca
     1621 tttatagatt gaaatataaa aaatgctcaa aagggaaata atcataataa attagcaggg
     1681 aaccaaagtc acaatctatt gaagggatag attagataca ataagggagt agttgaatga
     1741 ggaggagctc caagctcaga ctaccaaggg ggaagcgtag tccaaaggca agccctatga
     1801 tgatgggcat cgccatattt ctggttattc tagttccgac cgtattctat ctnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtcaactc cagcccgaat ctatcccagg
     1981 tccacctgga gaacaacggc atcacaaagc ttcataggat caaggtgacg gatcgggata
     2041 gatccctagg caccctctcc gagctcgacc caggggagaa gaaggtcctg tccataagcg
     2101 gcagagcgga aaagctatcg gtcttcgctc tggacccaac cggcctggag attcagggcc
     2161 gggtcaaata caactccaca aagcaagaaa agaatgcatc cgaggatgtc ggcctagaga
     2221 cgatattcga tccgctttta ttttccatgt ccattcccgc cggtggcgcc attccggcgg
     2281 aggcggaagt accaatagca gaagagcctg tcattcctgc catcccccag aactccagct
     2341 cctcttcttt agctctgaac atatcagtaa atagatcggc ctgcctggca ggagaggcga
     2401 taagctaccg atgcctggcc acaaacattg gccaggatga gctctccgat gttcgcatca
     2461 cctgtgcggg caagctcgca tccactggat ttatcactcc gcacaaagag ctggagctgg
     2521 atggcattct gctggtcgag aataacaccc gcctcctggc cgaggccagc gccaaagatg
     2581 gcaagggcaa tatctatacc aacaacacaa gcatcgatat ccgcatgatc tcccctcaga
     2641 tcaacctcct gctggaggct ccggagcttg ttcaccgggg ggagaacgcc tccatcctga
     2701 tccggatcga gaatagcggc gaggagaatc tgaccgacct gaaggtcgat gatggattcg
     2761 gagaggtgac caggattcct gcactcgcgg caggagaggc cgagatagtg caacagaata
     2821 ggacgattct ccacagcctg ccttatgagg tcatcgtatc cgcccgggac aaggtcggag
     2881 gagaggtcta ttccagccag agccagacga ttgcagtgat gaactcatcg ctcgagattc
     2941 agagcgagag cacgcaagtg gccacatatc ccggagagcc ggcagaggtg acctggatcc
     3001 tggagaacac tggccaggag actctgttca atgtcacgct gcaagggaac ggagcggact
     3061 gcattctgcc ccagatgctc ccgggaagat cggtacgaat ggcagccatc tacagcaaaa
     3121 atgacagcac ctggatcaat gttaccgcca ggggaatgga cggccgcggc tttgagacca
     3181 ccgccaatgg aagtgtgctt ttaagggcca tcaagcctgg aattagcctg aaggtcatgc
     3241 ctgaaaagat cgaggctgca ccaggtgacg aagcagagat cagctgtctg gtcaccaact
     3301 ccggcgagga ccgattgagg gaggtggtcc tcaccctgga tggcgcaaag ctcagctcac
     3361 tgggcgatct tgagccggga gagttcagag tggtccaagc aaggccgctc atcgaggaga
     3421 acagcaccct gcagttcgcc gttgctggag aagactccca gggcgaggtc tggtccgacc
     3481 ggatggcagt tgaggcga
//
LOCUS       DAOSGP010000137         3494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_24958_length_3494_cov_19.296889, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000137 DAOSGP010000000
VERSION     DAOSGP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24958_length_3494_cov_19.296889"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLV49_04820"
     CDS             complement(<1..759)
                     /locus_tag="PLV49_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98201.1"
                     /translation="MKRVLLVILILAALAISGCTENQDKAEKSVEELKALSLSAAENL
                     TSYSLKSTISNIRTLNDRGENATAENATIIRESAETEATVNLAGKQAHARGSTTIEVE
                     HRLLERNTTTNEADLYQMGNSTYIRDNGNWTHLQDPMTAEDIWKDGRNNQIKAMAETF
                     NLSLAELAGSENINGTDAYKLRIINGAAESDALYETAFSMAANLVSYPMFMPSVNRTE
                     LNETGRIEKTIWISKNTYIPVKYESKTSFKMTPII"
     gene            complement(819..1733)
                     /locus_tag="PLV49_04825"
     CDS             complement(819..1733)
                     /locus_tag="PLV49_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOG98202.1"
                     /translation="MERTLLLKGKVKEAYDLGDRLEFQFTDNISVFDKIIPSTIPFKG
                     ETLSREGVYWFERAHEMGIKTHFLQYLPPAGMLCKKVDILPTNRITPQSRNYLIPLEW
                     ICRWYVAGSLFDRVKAGEVAAQDLGFASGHVVRPGEELPEPFLEVSTKLEKTDRLIAK
                     EEALQISGLSPQEYEDARDIVLKIDEDINRSVASRGLIHADGKKELAFDMDRQIMIVD
                     VYGTADEDRFWDRKRYDEGELVDLSKEYVRQYYRQTGYKDRLYQAREEGKAEPAIPAL
                     PNDVVGETSRIYIKLFEMITGEKFKAKG"
     gene            complement(1815..3452)
                     /gene="thsA"
                     /locus_tag="PLV49_04830"
     CDS             complement(1815..3452)
                     /gene="thsA"
                     /locus_tag="PLV49_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HOG98203.1"
                     /translation="MAAGQLGGQPIIILKEGSQRTAGREAQKSNIMAAKAVAGAVRTT
                     LGPKGMDKMMVDNLGDVVITNDGVTILKEMDIEHPAAKMMVEIAKTQDAEVGDGTTTA
                     VVLAGELLKQAEGLLDQEIHPTVIAAGYRAAADKAMEILNKIAVSVTVNDADFLKKIA
                     ITSMTGKGSGTAREELAALAVEAVKAVVDEDGSVDTDNITVEKKVGGGITDSELVHGM
                     VIDKDRLHPNMPKKVTGAKIALLNAAIEIEKTEVDAKIEITSPDQLQAFLDQEESMLK
                     GMVDNVIKTGANVVFCQKGIDDLAQHFLAKEGIYTVRRVKKSDMEKLARATGGRVVTS
                     IHDLTEKDLGRAGLVEERKIGDEKMTFVEECENPKSVSIILRGGTEHVVDELNRAMED
                     ALRVVGVVVEDKMLVPGGGAPEVELALRLREYAATVGGREQLAIEAFADSMEVIPKTL
                     AENAGLDQIDSLVALRSQHEKGIKGSGLDMDTGVPVDMMKLGVVEPLRVKTQAINSAA
                     EAAVMILRIDDVIASKAGGPGGMPGGMPGGMGGEDFE"
BASE COUNT          641 a         1019 c          872 g          962 t
ORIGIN      
        1 gattattgga gtcattttaa agctcgtctt gctctcgtac ttgacgggga tataggtgtt
       61 ctttgagatc cagatggtct tctctatcct tcctgtctcg ttcaactccg ttcggttgac
      121 cgagggcatg aacatggggt aggagaccag attggcggcc atgctaaaag cggtttcata
      181 tagggcatcg ctctcagcag ctccgtttat gattcgcagc ttataggcgt ctgtgccgtt
      241 tatgttctcc gagccggcca attccgccaa agagagattg aaggtctcgg ccatggcctt
      301 tatctggtta tttctgccat ctttccagat atcctctgct gtcatcggat cctggagatg
      361 ggtccagttg ccattatccc taatgtaggt cgagttgccc atttggtaga gatctgcttc
      421 attggtggtg gtgttcctct ccaataaacg atgctccacc tcaatagtgg ttgagcctct
      481 ggcatgggct tgcttgccgg caaggtttac agtggcctcg gtctctgcac tctctcttat
      541 tatggttgcg ttctctgcag tggcattttc ccctctgtca ttgagcgttc tgatgttgga
      601 tattgtgctc ttgagactgt agctggtcag gttctctgcc gcggacaggg atagagcctt
      661 tagctcttcc acgctctttt ccgccttatc ctggttctcg gtacaaccgg atatagccag
      721 ggcagcaagt atcagaatta ccaatagcac tctcttcata tcatcatccc ttgataaatt
      781 tcctttaaat tttcgcttat tcaaaattag ccttaacctc atcccttggc cttgaacttt
      841 tcccctgtga tcatctcgaa cagcttgatg tagatccggc ttgtctcccc aaccacatca
      901 ttcggaaggg cgggtatagc aggttccgct tttccctctt ctcgggcctg gtagagccgg
      961 tccttgtagc ccgtctgacg gtagtactgg cgcacatact ccttggagag atcaacgagc
     1021 tctccctcat cgtagcgctt cctatcccag aaacggtcct cgtctgcggt tccatagaca
     1081 tctacgatca ttatctgtcg atccatatcg aaggccagct cttttttgcc gtctgcgtgg
     1141 atcaagccac gactggcgac gctgcggttg atatcctcat cgatcttgag aacgatatcc
     1201 cgggcatcct cgtactcctg aggggagagg ccggatatct gcaatgcctc ttcctttgca
     1261 atgagtcggt cggtcttctc cagcttcgtg gagacctcca ggaatggttc gggcagctct
     1321 tctccaggtc tcaccacatg ccctgatgca aagcccagat cttgagctgc tacttcgccg
     1381 gcttttaccc ggtcgaagag ggatccggcc acataccagc ggcatatcca ttccagggga
     1441 atcagatagt ttctggattg gggtgtgatc ctgttcgtgg gaaggatatc cacctttttg
     1501 cagagcatac cggccggcgg cagatactgc aaaaagtggg tcttgatgcc catttcatgt
     1561 gccctctcaa accagtagac cccctcccgg ctgagggtct cgcctttgaa gggaatggtg
     1621 ctcggaatta tcttgtcgaa gacggatata ttgtcagtga attgaaactc cagtcggtct
     1681 cccaggtcgt atgcctcttt gaccttgcct ttcaatagaa gcgtgcgttc cattgccgga
     1741 gatgaacctg gcaagaagat aacacttttg ctattatgtc tcgcaaactt aaaaaaagag
     1801 atgattggag aggtctattc gaaatcctct ccacccatgc cgccgggcat gccgccaggc
     1861 atgccgcccg gtccgccagc cttggaggcg atgacatcgt cgatcctgag gatcatgact
     1921 gctgcctcgg cagctgagtt gatggcctgg gtcttgaccc gcaggggctc gaccacaccc
     1981 agcttcatca tgtctacagg aacgccggta tccatgtcca ggccggagcc cttgataccc
     2041 ttctcatgct ggctgcgcag ggcaaccaga gagtcgatct ggtccagacc ggcgttctcg
     2101 gccagggtct tgggtatgac ctccatggag tcggcgaagg cctcgatggc cagctgctcc
     2161 ctgccaccga cggttgctgc atactctctg agacgaagcg ccagctctac ctctggagcg
     2221 ccgccgccgg gaacgagcat cttgtcctcc actacgaccc ccaccacccg cagggcatcc
     2281 tccatcgctc tgttcagctc atccactacg tgctcagtgc ctcctctcag gatgatagag
     2341 accgacttgg gattctcgca ttcctcgaca aaggtcatct tctcgtcgcc gatctttctc
     2401 tcttcgacca ggcctgctct tcccagatcc ttctccgtca ggtcatggat gctggtgaca
     2461 accctgccgc cagtggcgcg agccagcttc tccatgtcgc tcttcttgac ccggcgcaca
     2521 gtgtagatcc cctctttggc caggaagtgc tgggccagat catcgatgcc cttctggcag
     2581 aataccacat tggccccggt cttgattacg ttgtccacca tgcccttgag catgctctct
     2641 tcctgatcca ggaaggcctg aagctgatct ggggatgtga tctctatttt ggcatcgacc
     2701 tcggtcttct cgatctcgat ggcagcattg agcagggcga tcttggcccc ggttaccttc
     2761 tttggcatat tggggtggag cctgtctttg tcgatgacca tgccgtgaac cagctcggag
     2821 tcagttatcc ctcctcctac cttcttctcc actgtgatgt tatcggtgtc cacagagccg
     2881 tcctcatcca ccactgcctt aaccgcctcc acggccagcg cggcaagctc ctctctggcg
     2941 gtccccgatc ccttgcccgt catggaggtg atggcgatct tcttgagaaa atcggcgtca
     3001 ttgacagtga cgctgacggc aatcttattt aggatctcca tggctttgtc tgctgctgcc
     3061 ctgtagcccg cggcgatgac tgtgggatga atctcctggt ccagcagtcc ttctgcctgc
     3121 ttgaggagct ctcctgccag gacgacggca gtggttgtgc cgtccccgac ctcagcatcc
     3181 tgggtcttgg cgatctccac catcatcttg gctgccggat gctcgatgtc catctccttt
     3241 agaatggtga cgccgtcatt ggttattacc acatctccca ggttgtcgac catcatcttg
     3301 tccatgccct tggggccgag tgtggttctt acggcacctg cgactgcctt tgcggccatg
     3361 atgttcgact tctgtgcctc acgacctgca gttctctggc ttccttcttt aagaataatt
     3421 atgggctgtc cgcccagttg tcctgctgcc atttaatctc ctccgttgaa ttagttcaaa
     3481 gcgtgatatc cggt
//
LOCUS       DAOSGP010000138         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_25002_length_3490_cov_22.602329, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000138 DAOSGP010000000
VERSION     DAOSGP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25002_length_3490_cov_22.602329"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PLV49_04835"
     CDS             complement(<1..708)
                     /locus_tag="PLV49_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOG98204.1"
                     /translation="MKLLEEMSWPEIEEGLKVTRTVILPVGATEEHGPHLPTITDTIQ
                     ALETARTVAQKREVFLAPPIHYGVCRSTRGFPGTITVGHDALRSYVKELLISFTDSGF
                     ENVLILTGHAGSQHMSALKEACQMALEEREFRVSLVSLFDLIDPQAAETPHDGHAGEI
                     ETSMMMVIRKDLVKGLPEEHFPPRPRFLIMRDVRHLMGNGIMGNPAPATVKKGQSFLA
                     MAAEGLIEALEELESDCW"
     gene            complement(705..>3490)
                     /locus_tag="PLV49_04840"
     CDS             complement(705..>3490)
                     /locus_tag="PLV49_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HOG98205.1"
                     /translation="MSKRSVGKGDDGKKTAAGVQEAKPRDLPPTPLEKKAKSKSAAGL
                     WQNNDILIYAGLIVAAAVSLFLRTYYNWKLVFTDNGVVFSSETDAWYHMMLARGVVIN
                     HQRLWFDPMTNFPNGTTLHFGPFHSWAIALVSEILGLGNPSMHLVDTVGAIMPAVLGA
                     LLVFPIYFIGRELAGKSCGLISALIIAVIPGQVFSRTTLGFTDHHAAEILLSTIAMMF
                     LLMALRSGRRMTFDSLKDDWSKFKSPLIFSALSGLFLGLYIDAWSSGFLFVGVILFFV
                     FWQSIFDHMKGKSIDYLGIIAAIAFIVAIIPILPFVKPYYGFNHYYYSLFQPTSLLIG
                     AAAALVFALLSRYLNQKGLKRYYYPGSIAAATLIGTGILYIAIPQFTRPLLAGLSIFQ
                     QKTGGAATIGEASPLFANVNNVLTWFPGIGWIEGIFSFHTNSMIALLLSTFTFALVAL
                     LLLAFRNIWRQRPVDIAVLSWSLIMLVMILAQNRFGYYYGVNVAVLTAFLVVYILQKL
                     GIEDMDLDIDNLKDPSRLVRSNLKTIAAAIFIFALIILPSMSWSTVYARGASGPESDW
                     LTSTAWLKDNTPSPGMELYEKYQYPASGKYEYNDSAYGIMSWWDYGHLIEVVGHRIPN
                     ANPFQQGIGSVTQNTPGSSPFFLAENESQAEKVLAELDQNRSKYMNTKYVMVDQQMAT
                     GKFHAMAAWSGISNSNYVGGFLQEQGGEYGQFQIWREPYFKSMTARMFFFDGSEMAGN
                     QGIGLSYKGVEMEEGVIVPVLTEAPKISSNFTELEEFVRTSKEKGYMAEIGSTSPAVS
                     PVPLEALQHFRLVHESETPVTSSGQKWVKIFENVPGAVVKGSAPAGTPVMASIDIQTN
                     QNRMFEYRQSNVSNSDGQFVLVLPYSTEGPISGGTQFDTKAVGNYTLYVGNVVYGLRV
                     PEEYVLAGASINI"
BASE COUNT          781 a          943 c          939 g          827 t
ORIGIN      
        1 ccagcagtcg gactccagct cctccagagc ctctatgagc ccttccgccg ccattgccag
       61 gaagctctgt cccttcttta cggtagctgg ggcgggattt cccatgatcc catttcccat
      121 caggtggcgg acatccctca ttatcaggaa tctgggcctt gggggaaaat gctcctctgg
      181 cagtcccttg accaggtctt tgcgaatgac catcatcatc gaggtctcta tctctccggc
      241 atgcccgtca tgtggcgtct ctgctgcctg aggatcgatc agatcgaaca gagataccag
      301 gctgacccgg aattcgcgtt cctccaatgc catctggcag gcctctttta gggcggacat
      361 atgctggctc ccggcatggc cggttaggat caggacattt tcgaatccgc tatctgtaaa
      421 tgatatcaga agctctttca catatgagcg cagggcatca tgccccacgg tgatggtgcc
      481 tggaaatcct cgggtgctgc gacaaacccc gtagtggatg ggcggagcca ggaatacctc
      541 tcgcttctgg gcaacagtgc gtgccgtctc cagggcctgg atggtatcgg tgatcgtggg
      601 gagatgaggg ccatgctcct cagttgctcc cacgggcagg atcaccgttc tggttacctt
      661 cagcccctct tcgatctcag gccagctcat ctcctccaag agcttcatat gttgatgctc
      721 gctcctgcca gaacatactc ctctgggacc ctcagaccgt agacgacatt gcctacgtac
      781 agggtgtagt tccctaccgc cttggtgtca aactgcgtcc caccggatat agggccttca
      841 gtcgaatatg gcagcaccag gacaaactgg ccgtctgaat tgctcacatt tgattgtctg
      901 tactcgaaca tcctgttctg attggtctgg atgtctatgg aggccataac cggagttcct
      961 gccggagcac tccctttgac cactgcaccc ggcacattct cgaagatctt gacccacttc
     1021 tggccgctgg aggtgactgg cgtctctgac tcatgcacca ggcggaagtg ctgcagggcc
     1081 tctaagggta cgggggagac tgccgggctg gtggaaccga tctcagccat atatcctttc
     1141 tctttgctgg tccggacgaa ctcctccagt tcggtgaagt tgctgcttat cttaggcgcc
     1201 tcggtgagga cgggaactat cactccctct tccatctcca ctcccttgta ggacaatcct
     1261 attccctgat ttcctgccat ctccgatcca tcgaagaaga acattcgcgc agtcatggat
     1321 ttaaagtagg gctctcgcca gatctggaac tggccatatt ctccaccttg ttcctgcaaa
     1381 aatccgccga cataattcga attagatatg ccgctccatg cggccatggc atggaacttt
     1441 ccggtggcca tctgctgatc gaccatgacg tacttggtat tcatatactt agatctattc
     1501 tggtccagct cagccagcac cttctccgcc tggctctcgt tctccgccaa gaagaaggga
     1561 gatgatcccg gcgtgttctg ggtgacgctg cctattccct gctggaaggg attggcattg
     1621 ggaattctgt ggccaacgac ctctatcagg tggccgtaat cccaccagga catgatgccg
     1681 taagcagaat cattatactc gtacttgccg ctggcagggt attggtactt ctcatagagc
     1741 tccattcccg ggctgggggt attgtccttt agccaggcgg tggaggtcag ccagtcagat
     1801 tccgggccgc ttgcgcctcg agcgtagact gtgctccagc tcatgctggg cagtatgatc
     1861 aaagcgaaga tgaatatcgc agcggcaatt gtcttgaggt ttgaccttac aagcctggag
     1921 ggatccttca ggttatcaat atccaggtcc atatcctcga tgcccagctt ctggaggata
     1981 tacacaacca gaaatgccgt tagcacggcc acattaaccc catagtaata gccaaatctg
     2041 ttctgggcga ggatcatgac cagcatgatc agtgaccatg ataataccgc tatatccaca
     2101 ggcctctgcc tccagatatt cctgaatgca agcaggagaa gagccaccag ggcgaaggtg
     2161 aatgtcgaca ggagaagggc gatcatgcta ttggtatgga aggagaagat gccttctatc
     2221 cagccgatgc ctgggaacca ggttagaaca ttgttgacat tggcaaataa tggcgatgcc
     2281 tcgccgatgg tggcagctcc tcctgtcttc tgctggaaga tggacaggcc ggccagcagg
     2341 ggacgggtga actgggggat ggcgatatag agtatgcctg tgccgatcag cgtcgcagca
     2401 gcgatcgatc cgggataata gtatctcttg aggcctttct ggttgagata tcgggacaga
     2461 agggcgaaga ccaacgccgc tgccgcgcct atgagaaggc tggtcggctg gaagagactg
     2521 tagtaataat ggttgaatcc gtagtagggc ttgacgaagg gaagaatggg aatgatcgcc
     2581 actatgaagg cgatggctgc gatgatgccc aggtaatcga tgctcttccc cttcatatga
     2641 tcgaagatgc tctgccagaa gacgaagaag agtatcacgc ctacaaatag gaatccggac
     2701 gaccaggcat cgatgtacag ccccaagaaa agccctgaca gggcggagaa tatgagtggg
     2761 ctcttgaact ttgaccagtc atctttgagg gaatcgaagg tcatgcgcct tccagagcgg
     2821 agtgccatga gcaggaacat catggctatg gtgctgagca ggatctcggc cgcatggtga
     2881 tcagtgaatc ccaatgtggt gcggctgaat acctggccgg gaatgacggc gatgatcaat
     2941 gccgatatca gtccacagct cttgcctgcc aattcccttc caatgaagta gatgggaaag
     3001 accagaagcg ctcccagaac ggcaggcatg atggctccaa ccgtatccac cagatgcatg
     3061 ctggggttgc ccaggcccag tatttcagag accagggcga ttgcccagct gtgaaatggg
     3121 ccgaaatgca atgtcgttcc attggggaag ttggtcatgg gatcgaacca cagcctctga
     3181 tggttgatca ccactcctct ggccagcatc atgtggtacc atgcatcagt ttcgctgctg
     3241 aagacgacgc cgttatctgt gaataccaat ttccaattgt aataggtgcg caaaaagagc
     3301 gagactgctg cagcaacaat caatcctgcg taaatcagga tatcgttgtt ctgccacaag
     3361 cccgctgcgc tttttgattt tgccttcttt tccaaagggg taggcggcaa atcccgcggc
     3421 ttggcctcct gaacgccagc ggcggttttt ttgccatcat caccctttcc tacggatctc
     3481 ttagacatca
//
LOCUS       DAOSGP010000139         3472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_25193_length_3472_cov_14.192859, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000139 DAOSGP010000000
VERSION     DAOSGP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25193_length_3472_cov_14.192859"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLV49_04845"
     CDS             <1..216
                     /locus_tag="PLV49_04845"
                     /inference="COORDINATES: protein motif:HMM:NF037753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGF-CTERM sorting domain-containing protein"
                     /protein_id="HOG98206.1"
                     /translation="VEEAKEAVEEANKTVEEAEQVVEEAKEEVAEAEAKAEEAAEVPA
                     NQTPGFGSIFALTGLLAVAYLVLSRRD"
     gene            321..1340
                     /gene="amrS"
                     /locus_tag="PLV49_04850"
     CDS             321..1340
                     /gene="amrS"
                     /locus_tag="PLV49_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOG98207.1"
                     /translation="MRSTQEAQFWRRPDSESEDVNCSLCHQSCHIRPGKRGICAVREN
                     QGGMLMTLVYGNLIAANEDPIEKKPFFHFLPGSLAYSIATVGCNFRCLHCQNADISQL
                     PHETGKLPGNYVPPEDVVAGAVSRGCQSVAYTYTEPTIFFEYAYDVARLARASKLRNV
                     FVSNGYIGQDAADKIIPFLDGINIDLKGDDEFYRKVCKAKLDPVKSNIEKFWRAGVWV
                     EVTTLVIPGYNDSVEVLTDMAGFLAGISRDMPWHVSAFYPTYKLLDAPPTGVESIKRA
                     LNIGREAGLKYVYAGNIIGESESTICPDCGFVLIERRGYRVMKNSVTEGRCPNCKAAV
                     AGVWS"
     gene            1381..2589
                     /locus_tag="PLV49_04855"
     CDS             1381..2589
                     /locus_tag="PLV49_04855"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOG98208.1"
                     /translation="MPFCTVEEAIADIRAGRFIIILDDENRENEGDLMMAAEKVTPEA
                     INFMARFGRGLICMPMTAERLRELDIPLMTSQNTESMGTAFTVSVDARINTTTGISAF
                     DRATTVHALIDPVTRRSDIVTPGHLFPLQAKEGGVLRRTGHTEACVDLARLAGLQPAG
                     VIVEIMNDDGTMARLPQLERFAQEHGLKMLTIESLIRYRMQYEKLVCRISEVNMPTEY
                     GHFTAIGYESVLDGQCHIALVAGDPTCPDALVRVHSECLTGDVFSSKRCDCGEQLRRA
                     LQLISKNGNGVLLYMRQEGRGIGLANKLRAYALQDGGSDTVEANHRLGYPADLRDYGI
                     GAQILVDLGIKQIRLLTNNPRKVIGLEGYGLEIAERVPLEVEPNNVNRRYLETKRDKM
                     QHLLLQNEGQ"
     gene            complement(3111..>3472)
                     /locus_tag="PLV49_04860"
     CDS             complement(3111..>3472)
                     /locus_tag="PLV49_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG98209.1"
                     /translation="MEEVEGPNLKKGVIRPLADVHETEEALVVTMDMPGVEKQDIDIS
                     VVEDELRVSAQRKSEAEVNEQDYHRRERTYTRFERRVLLPDSIRTEEGRATLNNGVLQ
                     ITLPKVSVLNRKRIAID"
BASE COUNT          813 a          934 c          964 g          761 t
ORIGIN      
        1 gtagaagagg ctaaggaagc cgtcgaggaa gccaacaaga cggtagagga agctgagcaa
       61 gtcgttgaag aggccaaaga agaggtagct gaagctgaag ccaaagcaga ggaagctgca
      121 gaggtacccg ctaaccagac acctggattc ggcagcatct tcgccctgac tggccttctg
      181 gcagtcgctt acctcgtcct gagcaggaga gactgaaaga tcaaatagct ggaggagcta
      241 ggcttctcca gaactatctc tttttttatt tcatctaatc catatcatca ctattgcgtt
      301 tggttttctg gtgattatcc atgcggagca ctcaagaagc ccaattctgg aggaggccgg
      361 attcggagtc ggaggacgtg aactgcagcc tctgccatca gagctgccac atccgtcccg
      421 gcaagagagg aatatgcgct gtgcgtgaga atcaaggtgg aatgctaatg acgctggtct
      481 atggaaacct cattgctgcc aatgaggatc ccattgaaaa gaagccattc tttcatttcc
      541 ttcccggcag cctggcctac tccatagcca ctgtaggctg caacttccgc tgtctgcact
      601 gccagaacgc agatatctcc cagcttcctc atgagaccgg caaactgccg ggcaattacg
      661 ttccccctga ggatgtggta gcgggggcag tctccagggg ttgccagtcc gttgcttata
      721 cctataccga gcccacgatc ttctttgagt acgcctatga tgtggccaga ctggctcgag
      781 cctccaaact gcgcaatgtc tttgtcagca acggctacat tggccaggat gcggcggata
      841 agatcattcc cttcctggat ggcatcaaca tcgacctgaa gggagatgat gagttctacc
      901 gcaaggtgtg caaggcaaag ctcgacccgg taaagagcaa catagagaag ttctggcggg
      961 caggggtctg ggtggaggtc actaccctgg tcatcccagg ctataatgat tcagttgagg
     1021 tgctgaccga tatggcaggg ttcctggccg ggatcagccg ggatatgccc tggcatgtat
     1081 ccgccttcta ccccacctac aagctgttgg atgcaccgcc cacgggagta gaatcgataa
     1141 aaagagccct taacatcgga cgggaggcgg gattgaagta cgtttatgcc ggaaacatca
     1201 tcggagagag cgagagcacc atatgtccgg attgcggctt cgtcctaatc gaacgccgcg
     1261 gatacagggt catgaaaaac tcggtgaccg aagggcgctg ccccaactgc aaagctgcgg
     1321 tggcaggagt ctggtcctga caaaaatctt aatatctgac ccgctccgac tctgctccat
     1381 atgccatttt gcactgtaga ggaggcgatc gccgatatcc gcgccggtcg cttcataata
     1441 atattggacg acgagaaccg agagaacgag ggggacctga tgatggcagc ggagaaggtg
     1501 accccggagg ccatcaattt catggcccgc tttggccggg gtctgatatg catgccgatg
     1561 accgccgagc gccttcgcga gctggacatt cccctgatga ccagccagaa caccgagtcc
     1621 atgggcaccg cttttaccgt ttcggtggat gccaggatca ataccaccac cgggatttca
     1681 gcattcgacc gggcgacgac cgttcatgcc ctgatcgatc ctgtgaccag acgaagcgat
     1741 attgtcaccc ccggccatct gttccccctg caggccaaag aggggggagt gctgcggcgg
     1801 acggggcata cggaggcctg cgtcgacctg gcccggctgg cagggctcca gcccgctggg
     1861 gtgattgtgg agatcatgaa cgatgacgga accatggccc ggctcccaca gctcgagcgc
     1921 ttcgcccagg agcacggcct gaagatgctg accatcgaga gcctgatccg ctacaggatg
     1981 caatatgaga agctcgtctg caggatctcc gaggtgaata tgcccacgga gtacggccac
     2041 ttcacggcca ttggctatga gtccgtcctg gacggacaat gccatatcgc tctggtagca
     2101 ggagacccca cctgccctga tgccttggtc cgggtccact cagaatgcct cactggcgat
     2161 gtcttctcct caaaaagatg cgactgcggc gagcagctcc gccgggctct gcagctcata
     2221 agcaagaacg gcaatggcgt ccttctctat atgcgccaag agggaagggg cataggtctc
     2281 gccaataaac tgagagcata tgccctccaa gatggcggat cggatacggt agaggcgaat
     2341 caccggctgg gctacccggc agacctcagg gactatggaa tcggcgctca gatcctggtt
     2401 gatctgggaa taaagcagat tcgccttctg accaacaacc cgcgcaaggt gatagggctt
     2461 gagggctacg gcctggagat cgcagagcga gtacctttag aggtagagcc caataatgtc
     2521 aaccgccggt acctggagac caagagagat aaaatgcagc accttcttct ccagaatgag
     2581 gggcagtaaa aaagaaaggc tcttcgctga attatgtggg tcaggcactg cagtggtttt
     2641 agtctctgtt cctctgcgcc tctgtggttg ggtcaggatc gcggttcacc acagaaggcg
     2701 caaaagacac agagaatttg tgattcaagt ccatccgtgc taaatagcga ggagcctggt
     2761 tatactcgtc gtatataggt ttgattggat tacagataac ggattttaac cacaaagagc
     2821 acaaagatca caaaggcctc gcaaaggtta atccagttct tgatagccca ataatcattt
     2881 ggtgcggtct ttgtgtgctt tgtgtgcttt gtggtttatg gctcttcgct gaattatgtg
     2941 ggtcaggcac ggcagcggtc atggtctctg ttcctctgcg cctctgtggt tgggtcaaga
     3001 tcgcggttca ccccagatgc aaaaggatac agacaatttg cgattcaagt tcacccatac
     3061 cacatagcga ggagacaaaa gaaaaatagc tgatacgggg ttatctgtcc tcaatcgatg
     3121 gctattcgct tgcgattgag tacagagacc ttgggcaggg taatctgcaa gacgccgttg
     3181 ttgagtgttg cccttccctc ctccgtcctg atgctgtccg gcaggaggac tcttcgctcg
     3241 aaccgggtgt aggtcctctc ccggcggtgg tagtcctgct cgtttacctc agcctcactc
     3301 tttctctggg cagagacccg cagctcatcc tccaccaccg agatatctat atcctgcttt
     3361 tccactccgg gcatatccat ggtcacaacc aacgcctctt cagtctcatg aacatcagcc
     3421 aggggcctta tcaccccttt cttcaggttc gggccctcta cctcctccat aa
//
LOCUS       DAOSGP010000140         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_25338_length_3460_cov_18.022026, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000140 DAOSGP010000000
VERSION     DAOSGP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25338_length_3460_cov_18.022026"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..426)
                     /locus_tag="PLV49_04865"
     CDS             complement(1..426)
                     /locus_tag="PLV49_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98210.1"
                     /translation="MRHIILVLIALIALVGLMPGGMSKDPAPFDNGEINEAGDESLLT
                     TPSIAAFLNDSWAPTPFVAPLASAPLYAKKSFNNNTTNTTYAILDFLQNDTNNATNTS
                     LATYMVEGGNIYQFLKDDWNPSTPVEVVKGTPYTKGKMS"
     gene            complement(533..1447)
                     /locus_tag="PLV49_04870"
     CDS             complement(533..1447)
                     /locus_tag="PLV49_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOG98211.1"
                     /translation="MALVIGHRTARALAAENSLEGIRAAKRCLADYVHMDVRLSRDGK
                     LVLMHDDSVDRTTDGKGMVQDLNCIELKAIHAGNEQVPTLAEALSLAEELELGVVVEM
                     KEEGLEALVADVVPKEKSIITSSYHASLREIKEISDLKTGIIITSLPIKPVELALWAD
                     ADAIFPERVSPRLFKEAHQKGLEVYPGGVNTVAQAAWLLRLGADGLLTDDPCLIREAL
                     DQPVKATGQSNCEYYPCHHFEGQDCTHCFCPLYPCRDEELGRFVRTKRGKRFWSCIDC
                     KLVHRPIVAKYLSEHPESTTEELKALAD"
     gene            1533..2717
                     /gene="pscS"
                     /locus_tag="PLV49_04875"
     CDS             1533..2717
                     /gene="pscS"
                     /locus_tag="PLV49_04875"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HOG98212.1"
                     /translation="MLENLEKFTRLKRDVAQINVDPLQRGGILTPEARAALVEWGDGY
                     SICDFCAGMLDHIKNPPVEEFVHSILPEFLDIDEVRVTHGAREGKFAAMHCLCQKGDF
                     VVMDGNAHYTSVLAAERAGLNVEYVPPSKEPDYAISSEGYALAIERAYEKKGNVTLAL
                     LTYPDGNYGNLADARAIADVCHDYGVPLLLNGAYSVGRMPVKARELGADIIVGSGHKS
                     MAACGPVGVLGASEEYARIIFRPSATKKNKEVELLGCTVRGAPLITLMASFPTVVERV
                     GRWPEELENARWFSSEMEKMDLVQVGDRPHNHDLMFFLTERLYDISQTAKGGRYFLYR
                     EMKDRGVCGIKPGLTRQFKLSTYGLGRPELEVVLNALSEIIESHEKDEKGDKNEKGAN
                     GS"
     gene            complement(2714..>3460)
                     /locus_tag="PLV49_04880"
     CDS             complement(2714..>3460)
                     /locus_tag="PLV49_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98213.1"
                     /translation="GWHILPLMIFLICAAGPSLAQESDSSDAETVALDQTALIPENSS
                     IMDNESSNVSQSVPISSQSILDLSYIWSVSGIEIDNVIMALNQENEILYGQAKYEPDG
                     QGAWNAVVIGSVKEDNVDLVMTYLDDGSETSTRLNGTYDPSIQSIRGDLLKVKDGEIS
                     ERGTFEAIWINPDTASYTPAVMPQKAEPSDVQIEPSSSVAAMNETVAVKSGTRSEDYY
                     HDVRQDAVSILTGVGDLSQIPIGMGGSGLS"
BASE COUNT          775 a          894 c          968 g          823 t
ORIGIN      
        1 ctagctcatc ttgcccttgg tgtagggggt gcccttgacg acctcaaccg gcgttgaggg
       61 attccagtca tctttcagga actgatagat gtttccccct tcgaccatat aggtggctag
      121 gctggtattg gttgcattgt tggtgtcgtt ttgcaggaaa tccaagatgg catatgtggt
      181 attggtggtg ttattgttga aagatttctt ggcatacaga ggggcgctgg caagaggtgc
      241 cacgaatggg gttggcgccc agctatcatt caggaaagca gcaatggaag gagtagtcag
      301 cagagattca tcccccgctt cattgatctc tccattatcg aacggtgcag gatccttgct
      361 catgccgcct ggcataagcc caaccagagc aatcagagca atcagtacaa ggataatgtg
      421 tctcatgagt cctgcattat ccatcaggta tccataaagc tttccgtcgg taaaactata
      481 ttctcgatca agatcggtta tcctgaatgc attatggtga gatgagagtc gatcagtccg
      541 ccagggcctt gagctcttct gtagtcgatt ctgggtgctc tgacagatat tttgccacaa
      601 ttggccggtg aacgagcttg cagtcgatgc agctccagaa cctcttcccc ctctttgttc
      661 tcacgaacct gcccagctcc tcatccctgc aaggataaag gggacagaag caatgggtgc
      721 agtcctgacc ttcgaaatgg tggcagggat agtattcgca gttggactgg ccagtggcct
      781 tgaccggctg atctagcgcc tcccttatga gacagggatc gtctgtgagc agaccgtctg
      841 cccccaatcg gagaagccag gccgcctgag ctaccgtatt gacccccccc gggtagactt
      901 caagcccctt ctgatgcgcc tccttgaaaa gacggggact taccctttct ggaaatatgg
      961 catccgcatc agcccaaagg gccagttcta ccggctttat gggcagtgag gtgataataa
     1021 tgccggtttt aagatcggag atctccttga tctcgcgcag gctggcgtga taggaggagg
     1081 tgatgatgct cttttccttc ggcactacat cagccaccag ggcttccagg ccctcttctt
     1141 tcatctccac caccaccccc aactccagct cttctgccag ggatagagcc tctgccagag
     1201 tgggaacttg ctcatttccg gcatggatcg ccttaagctc aatgcagttc agatcctgga
     1261 ccattccctt gccgtctgtg gttctgtcca cgctatcatc atgcatcagc acgagctttc
     1321 cgtccctaga gagcctcaca tccatatgga cataatccgc aaggcagcgc ttcgcggctc
     1381 ttatcccttc caggctgttt tctgcggcaa gcgcgcgggc tgtgcggtga cctatcacca
     1441 gagccattga aatgaattcg cctggaaaat atatcacctt ttgcctgatt ctggcaaagg
     1501 attatactgc aagagggaca atcgatatgc aaatgcttga aaatcttgag aagttcacca
     1561 ggcttaaaag ggatgttgcc cagataaatg tcgatcccct gcagagaggt ggaatattga
     1621 ctcccgaggc aagggccgct ctggtggagt ggggcgatgg ctactccatc tgcgattttt
     1681 gcgccgggat gctggatcac ataaagaatc ctccagtgga ggagtttgtg cacagcatcc
     1741 tcccggagtt cctggacata gatgaggtta gagtcaccca tggcgcgcgg gaggggaagt
     1801 tcgctgccat gcattgccta tgccagaagg gggatttcgt ggtgatggat ggcaacgccc
     1861 attacacctc cgttctggca gcggagcgag cgggacttaa tgtggagtat gtgcccccca
     1921 gcaaggagcc ggattatgcc ataagctctg agggatatgc tctggctata gagagagcct
     1981 atgagaagaa gggaaacgta accctggccc tcctgaccta tcccgatggc aactacggta
     2041 atctggctga tgcgcgagcg atagctgatg tctgccacga ttacggtgtg ccgctccttc
     2101 tcaatggcgc ctactccgtc ggcaggatgc cggtgaaggc tcgggagttg ggggcagaca
     2161 taatcgtagg cagtggccat aagtccatgg ccgcctgcgg gccggtgggc gtcctgggag
     2221 cctcggagga gtatgcccgg atcatattca ggccctccgc taccaagaag aataaggagg
     2281 tggagctatt aggctgcact gtgcggggag cgcccctcat aaccctgatg gcctcattcc
     2341 caacagtggt agaaagggtg ggccgctggc ccgaggagct ggagaatgcc cgctggtttt
     2401 ccagcgagat ggagaagatg gatctggtcc aggtggggga tcggccgcat aaccacgatc
     2461 tgatgttctt tttgaccgag aggctctatg atatatctca gactgcaaaa ggaggtcgct
     2521 acttcctcta ccgggagatg aaggatagag gagtgtgcgg catcaagcct ggcctcaccc
     2581 gccagttcaa gctgagcaca tatggcttgg gccgtccgga gttggaggtg gtcttgaatg
     2641 cactctctga gatcatcgag tcccatgaaa aggacgaaaa gggagataaa aacgaaaagg
     2701 gagcaaatgg gtcttaagac agaccgcttc ctcccattcc aatgggtatc tggctgagat
     2761 ctccaacgcc ggtcaggata ctcacagcat cctgccggac atcatggtag taatcttctg
     2821 agcgggtgcc cgattttact gcaaccgtct cattcatggc ggccactgag ctgctcggtt
     2881 cgatctggac gtctgagggc tcagcctttt ggggcatgac cgcaggggta tagctggcgg
     2941 tatcgggatt gatccatatg gcctcgaagg tgccccgctc ggagatctcg ccatctttca
     3001 ccttcaagag atcgcctctt atcgactgga tcgaggggtc atatgtcccg ttcaggcggg
     3061 tggaggtctc tgagccatca tcaaggtagg tcatgaccag atctacattg tcctctttta
     3121 ctgatccaat gaccactgca ttccaggccc cctggccgtc cggctcgtat tttgcctggc
     3181 cgtagaggat ctcattctcc tgattgagag ccattatcac gttatcgatc tcgatacctg
     3241 aaaccgacca gatatagctc aggtccagga tagactgact ggaaatcgga acggattggc
     3301 taacgttgga agattcattg tccatgatgg atgaattctc cgggatcaat gctgtctgat
     3361 cgagcgctac cgtctctgca tctgagctgt ccgactcctg ggcgagcgaa ggaccggcag
     3421 cacagatgag aaagatcatc agaggaagga tatgccatcc
//
LOCUS       DAOSGP010000141         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_25448_length_3448_cov_17.612143, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000141 DAOSGP010000000
VERSION     DAOSGP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25448_length_3448_cov_17.612143"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLV49_04885"
     CDS             complement(<1..1136)
                     /locus_tag="PLV49_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG98214.1"
                     /translation="MAWLMLCSLPILACGSDLTLKIYGNANLDDTIDQRDVDYVEGII
                     NGTQEYSELADANNDGNIGQEDIAQIEDIVAGRETNLILKDTANRTVSIDMPVERVIV
                     PSGLTAEAFKVLKATDKVVGVSNAIHEKTYFFPEFADLPSVGGWKIEDYEAAISLDPD
                     LVISYEKWPPISEAEEKLDPAGIPVVVLEFTDPNFVTEELVKLSYILGTNGAEDRYIQ
                     WRSGYERQIDDYVNNLSRDERPSVFLETGFKGLNDVGTYGEGAKGSLVLELTGGRNIA
                     ANMSEAYPHVDSEWIITENPEVLMKYVYSSELDWGWNSTDEPEALIEEISSRDGWESI
                     GAVKNQRVYLISNELMTGLDSVVGYLYWVKLLHPEFAADPTEAY"
     gene            1338..2339
                     /locus_tag="PLV49_04890"
     CDS             1338..2339
                     /locus_tag="PLV49_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG98215.1"
                     /translation="MRKKALQILISLLLLISVCCGETVTLIDFTGHQVELSAPVTKTV
                     SLSAAASEILWALDGGKSMVGRSTSSNFPPMIESLTVAGKHSNSPDLELIMELEPELV
                     IADTMLSEEDRKVLEEDGIAVVVERFIDPHRTLLVIENLGRALGREERASELAGQLKG
                     YHDLIKERISGTNSEERPTVFFEWLGKPYHSASNGTSYDNFIDFAGGVNIAANESVDY
                     PDVSPEWVIEKDPDVILHILGSSKDYTEEELEQWRDEIISRPELQNSRAVKEGRVYVL
                     SGTVATGIRSAIGELYLAKWFYPELFEDTDPGVEHEKLIQEFYNMKLEGGYAYPSNA"
     gene            2332..3408
                     /locus_tag="PLV49_04895"
     CDS             2332..3408
                     /locus_tag="PLV49_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG98216.1"
                     /translation="MPEIAMVARKQCVVALMRTAFLVIIYIGLLSITGSGEMVTVVDF
                     TGHTVSVDAPIKSIVSLAGGASEIICALDEGASLVGRSTNSDFPPYLATVAEVGKNSY
                     SPDIELILELDPDLVVADTMISDGDKKTLEDAGIPVMEEKFIDPSRTILVMENLGQVL
                     GKEKRAQELVEFIKNYQDKIQDQVAALPEEDVPRVFFEWYGTPYHTVSSSGSYQNFIT
                     FAGGLNVAADLGNESNSYPDVSPEWVVQIDPDIILQSEPSTKNYTEDELAQLRDEIMS
                     RPELQSVKAVEDGRVFVMSGKITSGIRAIVGELYLAKWLHPQRFEDVDPDVVHRELID
                     KFYGLELEGAYAFPSSSFLDMEIE"
BASE COUNT          849 a          867 c          881 g          851 t
ORIGIN      
        1 ttatacgcct ctgtcggatc tgcagcgaac tccggatgga gcagcttgac ccagtaaaga
       61 tatcctacca cgctatccag ccccgtcatc agctcgttgc tgatcaagta tacccgttgg
      121 tttttcaccg cacctatgct ctcccatcca tcgcgacttg agatctcttc gatcaacgcc
      181 tcgggctcat cagtgctgtt ccagccccag tccaactctg agctgtaaac gtacttcatc
      241 aagacctctg gattctcggt gatgatccac tcgctatcca catggggata agcttccgac
      301 atattggcag caatgttcct gccgccggtc agttcaagca ccaggcttcc ctttgcccct
      361 tctccatatg tcccaacatc atttagaccc ttaaagcccg tctccaaaaa gacgctgggc
      421 cgctcatccc ggctcaaatt gttcacataa tcatctatct gcctttcata tccggagcgc
      481 cactgaatat accggtcctc tgctccattc gtgcccagaa tatagctgag cttgaccagc
      541 tcctccgtca cgaagttcgg atctgtgaac tccaggacga ctacgggtat tcctgcggga
      601 tcgagcttct cctctgcttc ggatatgggc ggccattttt cgtagctgat taccagatcc
      661 ggatcaaggg aaatggccgc ctcataatcc tctatcttcc agccgccgac tgatggcaga
      721 tcggcaaact cggggaagaa gtaagtcttt tcatgaatgg cattggagac gccgacgacc
      781 ttgtccgtcg ccttcaaaac cttgaatgcc tcagcggtaa ggccgctggg aacgatgacc
      841 ctttccacag gcatatcgat tgaaaccgtc cggttggcag tatccttcaa gatgagattc
      901 gtttctctgc ctgctacgat gtcctcgatc tgcgcgatgt cctcctggcc gatattgcca
      961 tcgttattgg catcggccag ctcgctatat tcctgcgtgc cattgatgat cccttcaaca
     1021 taatcgacgt ccctttgatc tatggtatca tccaggtttg cattgccata gatcttcagg
     1081 gtgagatcgg atccgcatgc caggatcggc agtgagcaca gcattaacca tgccattagt
     1141 atccatattc gcgtcttact aagcatttcg atcaccattt tttgattaaa ggaaagatta
     1201 attgatttaa gtctttctca tccggacaag gctgcatttg attcattacc tctcagtatg
     1261 acgcaccggc ccaagattta tatacaactt aatttgcctt aaaactaaat agtttgacta
     1321 aataggaggt agtaacgttg agaaaaaagg ctttgcagat cctgatctct cttttattgc
     1381 tcatatcagt atgctgcgga gagacggtga ccctaattga ttttactggg catcaggtcg
     1441 agctgtccgc tcccgtaacc aagacagtat ctctctcagc cgctgcttct gagatcctct
     1501 gggctctgga tgggggaaaa agcatggtcg gacgcagcac cagctccaac tttcccccaa
     1561 tgatcgagag cttaactgtc gcgggaaagc actcaaactc accggattta gagctcatca
     1621 tggagcttga acccgagctg gtaattgccg ataccatgct ctcggaggag gaccggaagg
     1681 tgctcgagga ggacggaatt gccgttgtcg tggaaaggtt catagatccc cataggacgc
     1741 tcctggtcat cgagaacctg ggaagggctc ttggaagaga ggagcgcgct tctgagctgg
     1801 cgggccagct gaaaggttat catgatctca taaaggagag aatctcgggc acaaattcgg
     1861 aggagagacc aaccgtgttc ttcgagtggc tgggaaagcc gtatcattcg gcatctaacg
     1921 gcacatccta tgacaacttc atagattttg ccggcggagt caacatcgca gccaatgaat
     1981 cggtcgatta tcccgatgtc agcccggaat gggtgataga gaaagatccg gatgtcatac
     2041 ttcacattct gggaagctcg aaggactata cagaggagga gctagagcag tggagggatg
     2101 agatcatatc tcggccggag ctgcagaatt caagggcggt caaagaggga cgggtctacg
     2161 ttttaagcgg tacggtggcc acgggaatcc gctctgcgat cggcgagctt tacctggcca
     2221 agtggttcta tcccgaatta ttcgaggaca cagatccggg tgtcgagcat gagaagctca
     2281 tacaggagtt ctacaacatg aagctggagg gaggatatgc ttatccctcc aatgcctgag
     2341 atagcaatgg tagcacgaaa acaatgcgtg gtggcattaa tgaggacagc ctttctggtt
     2401 attatctaca tcggcctcct ttccattact ggaagcggag aaatggtcac agtagtcgat
     2461 tttaccggac acaccgtgag tgtggatgcg ccgataaaaa gcatcgtctc tctcgcagga
     2521 ggagcttcag agatcatctg tgctctggac gagggagcga gcctggtggg acgttcgacc
     2581 aactccgact ttcctcccta tctggccacg gttgcagagg tggggaagaa ctcctattcg
     2641 cccgatattg agctgatatt ggagctggac cctgatctgg tagttgccga cacaatgatc
     2701 tccgacgggg ataagaagac cctggaggat gcgggaatac cagtaatgga ggagaaattc
     2761 atagatccct ccaggacgat cctggttatg gagaacctgg gtcaggtttt aggaaaagaa
     2821 aagcgagctc aagagctcgt agaatttatc aagaattacc aggataaaat ccaggaccaa
     2881 gttgctgctc tgccggaaga ggacgtccca agagtcttct tcgagtggta tggaacgccc
     2941 tatcataccg tttcatccag cggttcttat cagaatttca taacatttgc cggaggattg
     3001 aatgttgccg ctgacctggg aaatgaatcg aattcgtatc ctgacgtgag ccccgagtgg
     3061 gtagtacaga tcgacccaga tataatcctt cagtcggagc ccagcaccaa aaactacact
     3121 gaggatgagc tggcccagtt gagggatgag ataatgagcc ggccggagct tcagagcgta
     3181 aaggcggtag aagacggacg ggtctttgtg atgagcggca agatcacatc gggaatacgg
     3241 gccatagttg gagagcttta cctggccaaa tggcttcatc cacaacggtt cgaagatgtg
     3301 gaccctgatg ttgtgcaccg ggagctcata gataagttct acggtcttga gctggagggg
     3361 gcttacgcct ttccctcatc ttccttttta gatatggaga tcgaatgaga acaaccacac
     3421 tgttgatcgt ccttgctctt cttctagc
//
LOCUS       DAOSGP010000142         3431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_25633_length_3431_cov_18.399882, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000142 DAOSGP010000000
VERSION     DAOSGP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25633_length_3431_cov_18.399882"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PLV49_04900"
     CDS             <1..830
                     /locus_tag="PLV49_04900"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HOG98217.1"
                     /translation="PDMVVHLNLEKGEAELQVASLFLRGRYRKLVRGIPQTRWPCREC
                     RGRGCPRCNGTGRMYQESVDELIRPAVIQTAEAEDTVFHGAGREDIDARMLGSGRPFI
                     VEAVRPRIRTIDLAGLQENINRHAEGKVEVIELNVAYSAMVERLKEATFLKTYSALVR
                     LGAEVAEEKLKSGLKRLVGQVDQRTPTRVSHRRADKLRVRKVYSADLIEVVGNLARIN
                     VKGDSGLYIKELISGDGGRTQPNLAEILRVDAVVEELDVIDVGGESDGTSSRNAEEV"
     gene            814..1086
                     /locus_tag="PLV49_04905"
     CDS             814..1086
                     /locus_tag="PLV49_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HOG98218.1"
                     /translation="MRKKSRDKLSKTVRSSGISPVVRAIQEFDTGSKVHVLIDPSIHK
                     GMPHPRFHGKTAEVVGKRGRAFVLKVTDGDATKTLITLPEHLKAQK"
     gene            1088..1432
                     /locus_tag="PLV49_04910"
     CDS             1088..1432
                     /locus_tag="PLV49_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HOG98219.1"
                     /translation="MIVKSVLEEEFLTLAEVKEMLDQIREKRADEEELGYELRRAIRH
                     AELFSKGSAEENTAMVAELMKMEKMTRTIAVKIADIRPVTKDELRAIYAKERFNLSEE
                     ELSNILEIAFRG"
     gene            1438..2019
                     /locus_tag="PLV49_04915"
     CDS             1438..2019
                     /locus_tag="PLV49_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HOG98220.1"
                     /translation="MPEGRPPKREEIARVLDYLPYGRTPDSRTYQKQPLVQAVGETNF
                     VLMEMTPKEGVVPVAGARVYIGSGSRDVIDHVNRRIDYSELSNSAKLELPFVIQTIVL
                     EDEPRFIRYFNDAGPITTRMHALELLPGIGKKLMWSVLNERKKGSFKGFTDLVERVKG
                     LHNPEKLIAKRIEDELMDDRIKYRVFTTMPRIR"
     gene            2055..2759
                     /gene="rsmA"
                     /locus_tag="PLV49_04920"
     CDS             2055..2759
                     /gene="rsmA"
                     /locus_tag="PLV49_04920"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOG98221.1"
                     /translation="MDSIVSHADLGPEDWVLEIGPGEGILTRELAARAGRVYAVEIDP
                     DLAASLCNMAPNVMVIHADALTVRLPQYNKIVSNLPYNISSKITYRLLSQPFDLAVLM
                     FQKEFAQRLKAEPASKIYGRLGMVAGFFSHIEILQNVSRKAFRPVPDVDSAIVRLRPR
                     TERPQVDPRAFMRLADILFRNRRKKVKKGLAAYGVDKITLAELDQSLINMRPEELTPD
                     QVAGLILAIEKKSDWI"
     gene            complement(2796..>3431)
                     /locus_tag="PLV49_04925"
     CDS             complement(2796..>3431)
                     /locus_tag="PLV49_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG98222.1"
                     /translation="ACAQRAFSNMGQICISVNRVYAADEVAEEFTDRLVHQTERLRIG
                     NGLEDNVDLGPMFSNEQREKTKEHMKDALDRGAELLFGGREPEGKAFEKGYFFLPTVL
                     DRADHKMKIMREETFGPVAPIMRFETVDEAIKLANDSNYGLAAYIYTSDIATGIQAAE
                     RLEAGGVGVNINNVMDYQMPFGGWKESGIGRELGHYGLESFLEIKHIRLGI"
BASE COUNT          811 a          886 c         1006 g          728 t
ORIGIN      
        1 atccggatat ggtggtgcac ctgaacctgg agaagggcga ggcagagctg caggtggcat
       61 ctttattcct ccgcggccgc tacagaaagc tcgtcagggg tattcctcag acccgctggc
      121 cctgtcgcga gtgccgtggc cggggctgcc cacgatgcaa cggcacgggc cgcatgtacc
      181 aagagtcggt ggatgagctc atccggccag cggtgatcca gacggcagag gctgaggata
      241 ccgtctttca cggagccggt cgagaggata tcgacgcccg catgctgggc agcggcaggc
      301 cgttcatagt agaggccgtc cggccgcgga taaggaccat cgacctggcg gggctgcaag
      361 aaaatatcaa tcgtcatgca gagggcaagg tggaagttat tgagcttaat gtggcgtatt
      421 ccgctatggt ggaacggctt aaagaggcca cttttctgaa gacctactcc gccctggtca
      481 ggctgggagc agaggtcgca gaagaaaagc ttaaatctgg cctgaagaga ttagttggac
      541 aggtcgatca gcgtacgcca actcgggtgt ctcaccgaag ggcagataag ctcagggtga
      601 ggaaggttta tagcgcagat cttattgagg tagtgggcaa tctggctcgc ataaatgtaa
      661 agggcgacag cggtctttac atcaaggagc tgatctccgg agatggaggc agaacccagc
      721 cgaacctcgc ggagatcctc agagtggacg cagtcgttga agagctagat gtaatagatg
      781 taggtggaga atcagatggc acatcatcac ggaatgcgga agaagtctag agacaagctg
      841 agcaagactg tcaggagcag tggaatatct cccgttgtca gagctattca agagttcgat
      901 acgggatcaa aggttcacgt gctcatcgat cccagcatac ataagggcat gcctcacccc
      961 aggttccatg ggaagacggc cgaggtggtt ggaaaaaggg gcagagcatt cgtcctgaag
     1021 gtcacagatg gcgatgccac caagaccttg atcacactgc ctgaacatct taaggcgcaa
     1081 aaataagatg attgtcaaga gcgtattaga agaggaattc ctgaccctgg cggaggtcaa
     1141 ggagatgctc gaccagattc gagagaagcg agctgatgaa gaggagctgg gctatgagct
     1201 tcgccgggcg ataaggcatg ctgagctctt ttccaagggc agcgcagaag agaatacggc
     1261 aatggtagct gagcttatga aaatggagaa gatgacgagg accatagcgg ttaagatcgc
     1321 ggatatcagg cccgtcacca aggatgagct gagggcgatc tacgccaagg agcgattcaa
     1381 tctcagcgag gaagagctca gcaatatttt agaaattgca ttcagggggt aaggcgcatg
     1441 cctgagggga gaccgccgaa gagagaagag atagctaggg ttctcgatta tctcccctat
     1501 ggccgcactc cggacagtag aacctatcag aagcagcctt tggttcaggc ggtgggtgag
     1561 accaattttg tgctcatgga gatgactccc aaagagggag tggtccctgt agctggagcc
     1621 cgggtgtata tcggcagtgg tagccgtgat gtgatcgatc atgtcaaccg caggatcgac
     1681 tactcagagc tctctaacag cgccaagctg gagctgcctt ttgtcatcca gaccatagtg
     1741 cttgaggatg agcccaggtt catccgctac ttcaatgatg ccggccccat caccacccgc
     1801 atgcacgccc tggagctgtt gccgggtata ggcaagaaac tcatgtggtc ggtgcttaac
     1861 gaacgcaaga agggttcctt caagggcttt accgatctgg tggagagagt gaaaggcctg
     1921 cacaatccag agaagctcat agccaagcgc atagaggacg agttgatgga cgaccggatc
     1981 aagtaccggg tctttaccac aatgcctcgc ataagatgag actgggccag cattttttga
     2041 tcgatcgctc gattgtggat tctatcgtga gccatgccga tcttgggccg gaggattggg
     2101 tgctggagat agggcccggg gaaggcatcc taacacgaga gctggctgcc cgcgccggcc
     2161 gggtgtatgc agtggagatc gatcccgatc tggcggccag tctatgtaat atggccccaa
     2221 atgtgatggt tatacatgcc gacgctctga ctgttcgatt gccccagtac aataagatcg
     2281 tctccaacct tccctacaat atctccagca agatcaccta ccgtctgctc agccagccct
     2341 ttgacctggc ggtgctgatg ttccagaagg agtttgccca gcgattgaag gccgagcctg
     2401 ccagcaaaat ttacggtcgg ctggggatgg tcgccggctt tttcagtcat atcgagatcc
     2461 tgcagaacgt ctccagaaag gccttccgcc cggttcccga tgtagattcg gcgatagtcc
     2521 ggctccgccc gcgaacagag aggcctcagg tggatcccag ggcttttatg aggctggcgg
     2581 atattctctt cagaaacagg cgcaagaagg taaaaaaagg cctggcagca tacggggttg
     2641 ataagattac tctggccgag ctggaccagt ctctgatcaa tatgcggcct gaagagctga
     2701 ccccggacca ggtggcaggt ctgatattgg cgatcgaaaa gaagagcgat tggatctgat
     2761 cgggcaaagg ggcccgtttg ctccattccc tcttatcaaa tccccaggcg gatgtgcttt
     2821 atctccagga atgactccag gccatagtgc cccagctccc ggccgatgcc gctctccttc
     2881 cagccaccaa agggcatctg atagtccatc acattgttta tgttaacccc caccccaccc
     2941 gcctccagcc tttctgctgc ctgaatgcct gtggcgatgt cgctagtgta tatgtatgcg
     3001 gccaggccgt aattcgagtc gttagccagc tttatggcct cgtcaacggt ctcgaatctc
     3061 ataatcggag ctactgggcc aaaagtctcc tctctcatga tcttcatctt gtggtcggct
     3121 cgatccagga cggtgggcag gaaaaagtag cccttttcga atgccttccc ctccggctcc
     3181 cggccgccga agaggagctc tgctccccta tcaagggcgt ccttcatatg ctctttggtc
     3241 ttctcacgct gctcattgct gaacatcggc cccaggtcca cattatcttc caggccgttg
     3301 ccaatccgca gtcgctcggt ctgatggacc agtctatcgg tgaactcctc tgccacctca
     3361 tctgccgcat atacccggtt gacgctgatg cagatctgcc ccatattgga gaaggcccgc
     3421 tgggcgcagg c
//
LOCUS       DAOSGP010000143         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_25664_length_3428_cov_19.000000, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000143 DAOSGP010000000
VERSION     DAOSGP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25664_length_3428_cov_19.000000"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..645
                     /locus_tag="PLV49_04930"
     CDS             76..645
                     /locus_tag="PLV49_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HOG98223.1"
                     /translation="MLKANCRTSWNSTEIMPQIHSSSYVDESATIIGDVRVGKDVYIA
                     PSVSLRADEASPIIIGDECNIQDCAVFHGLKGSSIELGENVSIAHGAVIHGPILMGDE
                     CFVGFNSVVHASTLGKKCFVGHLAAVIGVTLAEGSFVPHGRVVDTQEKADALGRVPES
                     LKDFNEEVVEVNCEFAKSYGIRRRSCVGQ"
     gene            746..1774
                     /gene="asd"
                     /locus_tag="PLV49_04935"
     CDS             746..1774
                     /gene="asd"
                     /locus_tag="PLV49_04935"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOG98224.1"
                     /translation="MAKIKAGVLGATGAVGQRFIEGLKSHPWFELTSLAASERSAGKK
                     YREAAKWRLESELPDDIAEMTVVNVDPREVDADIVFSALPAADAMSVEPAFAAAGCAV
                     ASNTSSYRMVEDVPLLIPECNHEHLDIIKVQREKRGWEGFLTTNPNCTTIMFTLPLKP
                     LMKFGIESVHVASMQAVSGAGYEGVPSMAILGNVIPYIGGEEEKVETEPQKILGHFDG
                     ERIINADFTISASCHRVPVMDGHTEAIWVKMRDNPTPEEVRQAMLDFDPGLGELPTSP
                     KKALIVKDEPDRPQPRLDRMRGNGMSISVGRIRSGIRFICIGHNTVRGAAGASILNAE
                     LLVKKGLL"
     gene            1814..2779
                     /locus_tag="PLV49_04940"
     CDS             1814..2779
                     /locus_tag="PLV49_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98225.1"
                     /translation="MLIIAWELTAACNLSCQYCRASASHEQDLGELDTDEAKRFVESI
                     APRKPMLILSGGEPLLRPDLFQIIRLAVSLGIRVSLASNGTLITPELAKEIADSGVSR
                     VSISLDGADAAMHDLGRGQGSFKRSIRGIENLRGRVDFQINFTVTQKNQSELIRIFDL
                     AEKLGAAALHIFFLVPTGRGREEDVITPVRQEEMLRQIEGEMDRRRLEVQVTCAPQYA
                     RLKRPGHERGSGGCLAGRRFVFVSRKGEVYPCGYLPLRVGSVREKNFIEIWENSPELQ
                     ALREGRLKGKCGRCEFSRSCGGCRARAYALTGDYLQSDPSCRLEI"
     gene            2782..3246
                     /locus_tag="PLV49_04945"
     CDS             2782..3246
                     /locus_tag="PLV49_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOG98226.1"
                     /translation="MDEIDLRLLSLVQEGFPLTPKPFEYLGGLLSLEEKEVIERLDGL
                     QKDGLVRRIGPILDLRRLGRSGILAALQVSPEEADSLAEFVNQYQEVSHNYLRPNESG
                     YNMWFTVSATEERIQEVLEEIRKSTGRKMLVLPTGRIFKIGVKFDIMCEEEE"
     gene            3246..>3428
                     /locus_tag="PLV49_04950"
     CDS             3246..>3428
                     /locus_tag="PLV49_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG98227.1"
                     /translation="MTKPILMDFYADWCGPCKQQGPIFEALAEKMKDVADFKKINVDR
                     EGDLAMEKGIFVVPTLI"
BASE COUNT          813 a          866 c         1041 g          708 t
ORIGIN      
        1 atagctcctt ttgcaatcgt cgttaatggc aatctctttt aaggtgaatg gagaatatgc
       61 gaaaaggtgg taaatatgct caaggccaat tgcagaacct catggaatag cactgagatc
      121 atgccccaga tacattcctc gtcctatgtg gatgagtcgg cgacgatcat aggcgatgtg
      181 cgcgtcggca aagatgtcta tatcgctcca tccgtatcct tgcgggcaga tgaagccagc
      241 cccataatca ttggtgatga atgcaacatt caggactgcg ccgtctttca cggcctcaag
      301 ggaagctcca tcgagctggg agagaatgtg tccatcgccc atggagcagt cattcacgga
      361 cccatattga tgggagacga gtgttttgtg ggcttcaact ccgtggttca cgcctcaacc
      421 ctgggaaaga aatgctttgt cggccacctg gcagcagtca taggcgtcac tctggcggag
      481 ggcagtttcg tgccccacgg tagagtcgtc gatactcagg agaaggcgga tgctcttggc
      541 cgcgtgccgg agagcctcaa ggacttcaat gaggaggtgg tggaggtaaa ctgcgagttc
      601 gccaaaagct atggcattcg gaggagatcc tgcgtggggc agtgaccccc cttcgaaaat
      661 cccagaactt tttttgctcc atcatccgga caataaatat atacatccct agcttaagtc
      721 cccatcagaa tttaggtgat ttataatggc caagatcaaa gcaggcgtct taggtgccac
      781 cggtgccgtt ggacagcgtt tcatagaagg actgaagagc catccctggt ttgagctcac
      841 cagccttgcc gcatcggaga ggagcgcggg caagaagtac cgcgaggcgg caaaatggcg
      901 tctggagagc gagcttcccg atgacatagc agagatgacg gtggtcaatg tcgatccgag
      961 agaggtggat gctgatatag tcttctccgc tcttcccgcc gccgatgcca tgtctgtaga
     1021 gcctgccttc gctgctgccg gctgtgccgt ggccagcaat accagctcat atcgcatggt
     1081 ggaggatgtc cccctgctca taccggagtg caaccacgag cacctggaca taataaaggt
     1141 tcagagggag aagagaggct gggagggatt cctaaccacc aatcccaact gcacaaccat
     1201 catgttcacc cttcctctca agcccctgat gaagttcggg atagagagcg tgcatgtggc
     1261 ctccatgcag gcggtgagcg gagcaggcta tgagggcgtg ccctccatgg cgattttagg
     1321 gaacgtcatt ccctatatcg gcggggagga ggagaaggtt gagacggagc cgcagaagat
     1381 cctgggccat tttgacggtg agaggatcat caacgccgac ttcaccataa gcgcaagctg
     1441 ccaccgggtt ccggtgatgg acggccacac cgaggccatc tgggtgaaga tgagagataa
     1501 ccccacccca gaagaggtga gacaggccat gctggacttc gatcccggcc tgggagagct
     1561 gcccacctcg cccaagaagg cgctcatagt caaggacgag ccagaccggc cccagcccag
     1621 gctggaccgc atgcgaggca acggaatgtc catttctgtg ggcaggatca ggtcgggaat
     1681 caggttcatc tgcattggcc acaataccgt ccgcggtgca gcaggtgcga gcatactcaa
     1741 tgccgagctg ctggtcaaga aaggactgct ttaaattggc ggcaattgaa aagcagtatg
     1801 ctctgatcgc aacatgttga tcatagcctg ggagctgacg gctgcctgca acctatcctg
     1861 tcagtactgc cgcgcttctg cctctcatga gcaggatctg ggcgagctgg acacagatga
     1921 ggccaaaagg ttcgtagaga gcattgctcc ccggaagccg atgctcatcc tcagcggggg
     1981 agagcctctg ctgcggcccg atctatttca gatcatcagg cttgccgtat ctcttggcat
     2041 ccgggtctca ttggccagca acggaaccct gataaccccg gagctggcga aggagattgc
     2101 cgactccggg gtcagcaggg tgagcatcag cctggatggg gcagacgctg cgatgcacga
     2161 ccttggccga ggccagggga gtttcaagcg gtcgattagg gggatagaga atctccgcgg
     2221 ccgggtcgac ttccagatca acttcacagt cacccagaag aatcaatccg agctcatccg
     2281 gatcttcgac ttggcggaaa agctgggtgc agcagcgctt cacatcttct ttctggtacc
     2341 tacgggccgg ggaagggagg aggacgtcat tactcctgtg cggcaggagg agatgctccg
     2401 ccagatcgag ggagagatgg atcgaaggag attggaggtg caggtcacct gcgctcccca
     2461 gtacgcccgc ctgaagaggc cgggccatga aagaggaagc ggagggtgtc tcgccggaag
     2521 gaggtttgta ttcgtatccc gcaagggtga ggtctatccc tgcggctatc ttcctctccg
     2581 ggtcggaagc gtcagggaaa agaattttat agagatatgg gagaactctc ccgagcttca
     2641 agccctgcgt gagggaaggc tgaagggaaa atgcggccgg tgcgaatttt cccgaagctg
     2701 cggcggctgc cgggcccggg cctatgccct gactggggat tatctgcagt ccgatccttc
     2761 atgccgcctg gagatataag gatggatgaa atcgatcttc ggctgctctc cctggttcag
     2821 gagggatttc ctcttactcc caaacccttc gagtatctgg gaggactcct aagcctggag
     2881 gagaaggagg tcattgaaag gctggacggt ctgcaaaaag atgggctagt gaggaggatc
     2941 ggccccatcc tcgacctgcg caggctggga cggagcggaa ttctggccgc tcttcaggtc
     3001 tccccagaag aagcggatag tttggctgag ttcgttaatc agtaccaaga agtctctcat
     3061 aactacctcc ggccaaatga gagcggctac aatatgtggt ttaccgtctc agcaactgag
     3121 gagcgcattc aagaggtgct ggaggagatc agaaaaagca ccgggcgaaa gatgctggtt
     3181 ctccccacgg gcaggatatt taagatcgga gtcaaattcg atataatgtg tgaagaggag
     3241 gaataatgac aaagccaata ctgatggact tttatgcaga ctggtgcggg ccgtgcaagc
     3301 agcagggtcc catcttcgag gcattggccg agaagatgaa ggatgtggct gatttcaaaa
     3361 agatcaatgt tgaccgggag ggagacctgg ccatggagaa gggcatattt gtggtgccca
     3421 cattgata
//
LOCUS       DAOSGP010000144         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_25665_length_3428_cov_14.930626, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000144 DAOSGP010000000
VERSION     DAOSGP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25665_length_3428_cov_14.930626"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PLV49_04955"
     CDS             <1..1062
                     /locus_tag="PLV49_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG98228.1"
                     /translation="LASYPKTFTDTQGREITLDESPQRIITRAPDEARIVIALGYGDK
                     LVAGEQATKSCLCPMTFSNTTEGCLECYENIIDGRMPDLPEISTRYDIYFEEIAKLEP
                     DLIFCGNLKDAEAFEDKIGCPVVVLGGQGWNFGDDGYFDSIRVAGEALDARDQADELI
                     AFAKGKVEMIESVTKEIDPDDRPKVYFASRGAGSGFYDPKEGRDFTRTEPKYDPLTIA
                     GGNNIASEINGSTVNVALEQIIAWNPDFIFISNSNAGNNTGLDFIKNSPELASINAIK
                     EGNVYNCFYPHCRGTPPDRNLFNMMYMAKVMYPEEFKDLDLEKEGNEIFKAFLGVDGV
                     FSEYADYLVWPREYLDSLK"
     gene            1071..1916
                     /locus_tag="PLV49_04960"
     CDS             1071..1916
                     /locus_tag="PLV49_04960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG98229.1"
                     /translation="MPGIINWDELWKATHAGGFHHHGRDGQDLASHWDSKARGFNKRV
                     MKNTERAINQVATLGLLPHETVLDVGAGTGRLAIPMARVARSVTALDQSKGMLACLEE
                     NMAKEGIENIRSIHKGWEELAEGEIEPHDVVLSSNSLGVLNLRESLAKMDSMAKRAVY
                     IFTFADRRRDDGFADFLRRGKRARHHFGGPADYLVIYNLLADMGIFADINIMNWQSSE
                     HYSDLDEAVAEWKEMHEIPAEREPELREFLSGKMTESEQGLCMHRHHKQAMISWQKDQ
                     PASEH"
     gene            1937..2719
                     /locus_tag="PLV49_04965"
     CDS             1937..2719
                     /locus_tag="PLV49_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOG98230.1"
                     /translation="MVSIPILAGSALLIVILVSSIRVVRQYERGVIFRLGKIKKERGP
                     GLFPLIPLADKMVRVDMRVRELDVPKQTVISKDNVTLEVDAVIYYKVMDASRAIIEVE
                     DFEAATLLLAQTTLRDILGQNELDTILSDRDDLNKRIKEILDSTTGPWGMHVVMVTMR
                     DVSLPENMLRAIARQAEAEREKRARIILAEGEYQASKMMNLAADMYEDKPSALKLREF
                     QTLTEIAKEKNLIVVSTGSDDGHRNSDLPLFMGLARAAVSNK"
     gene            2716..>3428
                     /locus_tag="PLV49_04970"
     CDS             2716..>3428
                     /locus_tag="PLV49_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG98231.1"
                     /translation="MTSFRALFFRGKPDAGYSRAKRSGKKSNHELAHDRFKFIRILFV
                     LGIAGLLVLLTGLIITLGPLDISVLDSYSALLARFWPEHFYVDELTSRVVWNIRFPRI
                     MGGIMAGFGLGICGCVMQAVLKNPLASPFTLGITAGAQFGISLAAVIGFSFLAGPYFI
                     IGNAFLCALFCSLFIIGLAAIKGATSENLVLAGIAVNYFFTAISQLLKYFATDEQLRL
                     MTNWGMGDLAAFSWTNSRIL"
BASE COUNT          807 a          948 c          987 g          686 t
ORIGIN      
        1 ctggcatcat accccaagac ctttaccgac acccagggcc gggagatcac tcttgatgaa
       61 tccccgcaga ggataatcac cagagccccg gacgaggcca gaatcgtgat cgccctgggc
      121 tacggggaca agctagtcgc cggtgagcag gccaccaaat cctgcctctg ccccatgacc
      181 tttagcaaca ccaccgaagg ctgcctggag tgctatgaga acatcattga tggccggatg
      241 cccgaccttc ctgagatcag caccaggtac gacatctatt tcgaggagat cgccaagctg
      301 gagcctgacc ttatattttg cggaaacctg aaggatgctg aggcctttga ggacaagatc
      361 ggctgcccgg tggtagttct tgggggacag ggctggaact tcggcgacga tggctacttc
      421 gatagcatcc gcgtggccgg cgaggccctg gatgctcggg atcaggccga tgagctcatc
      481 gcctttgcca aaggcaaggt ggagatgatc gaatcggtga caaaagagat agatcccgat
      541 gatcggccaa aggtctactt cgcttccagg ggcgctggtt ccggatttta tgatcccaaa
      601 gagggaaggg acttcaccag aaccgagccc aaatacgatc ctctgaccat cgccggcgga
      661 aacaacatcg cctcggagat caacggctcc acagttaatg tggcccttga gcagattata
      721 gcctggaacc ctgacttcat attcatatcc aacagcaatg cgggaaacaa taccggcctt
      781 gatttcatca agaacagccc tgagctggcc tccatcaacg ccataaagga aggcaacgtc
      841 tacaactgct tctatcccca ctgccggggc acaccgcctg acagaaacct gttcaatatg
      901 atgtacatgg ccaaggtgat gtacccggag gagttcaagg atctcgacct ggagaaagag
      961 ggcaatgaga tattcaaggc cttcttggga gtggacggcg tcttcagcga gtacgccgac
     1021 tacctggtct ggccaagaga gtacctggac agcctgaaat aggtgctcct atgccgggaa
     1081 tcattaactg ggacgagctg tggaaggcaa cccatgcagg cgggtttcac catcacggcc
     1141 gggacggcca ggatctggca tcccactggg atagcaaggc ccgaggattc aacaagaggg
     1201 tgatgaagaa tacggagagg gcaataaatc aggtggccac cctcggcctc ctccctcatg
     1261 agaccgtact ggatgtgggc gccggcacag gcaggctggc gatccctatg gcccgggtgg
     1321 ccaggagcgt aactgctctt gatcaatcca agggaatgct ggcctgcctc gaggagaaca
     1381 tggccaaaga gggcatagag aacatccgct ccatccataa gggctgggag gagctggctg
     1441 agggtgagat cgagccgcac gacgtggtct tatcctccaa ctctttgggg gtgttgaacc
     1501 taagagagtc cctggccaag atggactcta tggccaaaag ggcggtctat atcttcacat
     1561 tcgccgatcg gaggagagat gacggctttg cagacttcct gaggcgaggc aaaagggccc
     1621 gccatcattt cggcggccca gccgactacc tggtgatata caacctcctg gcggatatgg
     1681 gaattttcgc cgacatcaat atcatgaact ggcagtcctc cgagcactac tcggaccttg
     1741 acgaggcggt agctgagtgg aaggagatgc acgaaatacc agcagaaaga gagcccgagc
     1801 tgagagagtt tctctccggg aagatgactg aaagtgagca ggggctgtgc atgcaccgcc
     1861 atcataagca ggccatgatc tcctggcaga aggaccagcc tgcgtctgag cattagatgc
     1921 aggggaggac aatacaatgg tctccattcc cattctggcc ggatcggctc tgttgatagt
     1981 aatactggta tcgagcatcc gggtggtgag gcagtacgag cgaggggtga tattccgcct
     2041 gggcaagatc aagaaggaga gaggtccagg ccttttcccc ctcattcctc tggcggataa
     2101 gatggtccgg gtggacatga gggtaagaga gcttgatgtc cccaaacaga cggtgatcag
     2161 caaagacaat gtcacccttg aggtggatgc ggtcatctac tacaaggtga tggacgcctc
     2221 tcgagcgatc atcgaggttg aggacttcga ggccgccacc cttcttctgg ctcaaaccac
     2281 cctgagagat atcctgggac agaatgagct ggacaccatc ctctccgatc gggacgatct
     2341 caataagagg atcaaggaga tcctggactc gaccaccggt ccctggggca tgcacgtggt
     2401 gatggtcacc atgagggacg tctcattgcc ggagaacatg ctgcgcgcca tcgcccgcca
     2461 ggcagaggcg gagagagaga agagagcccg gatcatcctg gcagaaggag agtatcaggc
     2521 ctcgaagatg atgaacctgg cagcagatat gtacgaggac aagccgagcg ctctgaagct
     2581 gagggagttc cagaccctga ccgagatcgc taaagagaag aacctgattg tggtctccac
     2641 cggatcagac gatggtcaca ggaattcgga ccttcccctt ttcatggggc tggccagggc
     2701 ggctgtgagc aataaatgac cagctttcgg gcactcttct tccggggcaa gcccgacgct
     2761 ggctactctc gggcgaagag gtcgggcaag aagagcaacc acgagctggc ccatgacaga
     2821 ttcaaattca tcagaatctt attcgtgctg ggcatcgccg gccttctggt ccttctcacc
     2881 gggctcataa tcaccctcgg ccccctggat atatccgtgc tcgactccta ttccgccctt
     2941 ctggcccgct tctggccgga gcacttctat gtggatgagc tcaccagccg ggtggtctgg
     3001 aacatccgct ttcccagaat catgggagga atcatggccg gctttgggct ggggatatgc
     3061 ggctgcgtga tgcaggcggt cttaaagaac cctctcgcca gccccttcac cctgggaatc
     3121 actgccggtg cccagtttgg catatccctg gccgcagtca tcggcttttc atttcttgcc
     3181 ggtccctatt tcataatcgg taatgccttc ctctgcgccc tcttctgctc tctgttcatc
     3241 atcggcctgg ccgccatcaa aggagcaacc tctgagaacc tggtcctggc aggaatcgcc
     3301 gtcaactact tcttcactgc gatcagccag ctgctcaagt acttcgccac cgacgagcag
     3361 cttcgcctca tgacaaactg gggaatgggg gacctggctg ccttctcctg gaccaactcc
     3421 aggatact
//
LOCUS       DAOSGP010000145         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_26546_length_3349_cov_13.146327, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000145 DAOSGP010000000
VERSION     DAOSGP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26546_length_3349_cov_13.146327"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..117
                     /locus_tag="PLV49_04975"
     CDS             1..117
                     /locus_tag="PLV49_04975"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HOG98232.1"
                     /translation="MAGSRKHLRSYVADLKEQEIEYEPHHLERSEFEETVRV"
     gene            325..1185
                     /locus_tag="PLV49_04980"
     CDS             325..1185
                     /locus_tag="PLV49_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG98233.1"
                     /translation="MLQDLCLTIVVENSSSLENDHLQAQHGLSILLDLDMNLGSENMK
                     LLWDTGATPDVMLHNADALHIDPEEIDLICLSHGHYDHTGGLMGVLQRRKSPVPIITH
                     PDIFAPKLKVRPFLKYIGAPFTRDQLEAAGAIMLPCRSPVTLAPGVMTTGEVPRVEAF
                     ETVEGFWTMKNDQNTPDAMPDDQSLAIHLPGKGLVVISGCAHSGIINTIRYAQRITGE
                     ERLYAVIGGFHLMDADNKRIDATARALLDLDPAIVRPGHCTGQKALSRLQDILGESCQ
                     PLTVGDSIEF"
     gene            1198..2166
                     /locus_tag="PLV49_04985"
     CDS             1198..2166
                     /locus_tag="PLV49_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98234.1"
                     /translation="MKQLIAFGPVPSRRLGRSLGINNIPAKICSYSCAYCQVGRTLQM
                     SAERRSYYLPQQIFDQVAQKVAEARDLGLTIDYLSFVPDGEPTLDSNLGEEIDMLKTL
                     GIKIAVISNASLIWDEKVREDLFKADWVSVKVDSLEDKAWRRIDRPHRSLRLDTILQG
                     IKDFAREYSGILATESMLLAGINDCKASLLALAEFLEGIDPEVSYISVPIRPPTESWV
                     QMPSPENLCLAYQIFSQRLKRVELLVGYEGDSFDFTGEAKEDILAIASVHPMREAALR
                     RFLEKSGNSWDIIEELLEKKELLRIEYQGNSFYLRRPERPDKIGAE"
     gene            complement(2336..2935)
                     /locus_tag="PLV49_04990"
     CDS             complement(2336..2935)
                     /locus_tag="PLV49_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98235.1"
                     /translation="MSAKNTVALGGLMIYLMTSFFVQAQAYDPLGSDIFLMPGESRAI
                     AGQDNVKTYIDPNADPLSIDPLMRPIDPQHSAEYLDKISGREYLSPFVQITQDLRGNW
                     RLNLYGSLPGTVDLTLVQNKDAVFGRGMLTSFGTTKDLSASGWTAKDVLYLDMVDMQN
                     MMLYRCTLTMSKDNLSGSFNAYDAQGGAWSGTIQGSRQA"
     gene            complement(2991..>3349)
                     /locus_tag="PLV49_04995"
     CDS             complement(2991..>3349)
                     /locus_tag="PLV49_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOG98236.1"
                     /translation="DGSPGIGCPVIASLTGVDLALVVTEPTVTGEHDLVRLLDVAGHF
                     GIKTAVCINKYDLNPEAARQIEELCGKRGVKIAGRLPYHTSVIDAMVKALAVVEIGGP
                     VAEAIEDMWKGLERQL"
BASE COUNT          811 a          921 c          880 g          737 t
ORIGIN      
        1 ctggcgggct caagaaagca ccttcgctct tatgtcgccg accttaaaga gcaggaaata
       61 gaatacgagc cgcaccatct tgagaggagc gagtttgaag agacggtgcg ggtataaata
      121 gggctgcatt ttttgcggta tgagccgggt tagtcttgca gatccggagg gatgagctgg
      181 taatgctgcc tgccatacag gctaaaagcc gggactaccc tttgggaaag ccacaatcac
      241 tgccatactg aaaagacagg aaatatagac aaaaagcggc cctgacgcgc catccttaag
      301 ctccgatcag tcatatatca atccatgctc caggacctct gtctgaccat tgtggttgaa
      361 aactcctcat cccttgaaaa cgaccatctg caggcccagc acggcctgtc catcctcctg
      421 gatctggata tgaatctggg atcagagaac atgaaacttc tctgggacac cggggccacc
      481 ccggacgtga tgctccataa tgccgatgct ctgcatatag atccagagga gatcgacctg
      541 atctgcctca gccacggcca ctacgatcac actgggggac tgatgggcgt gctgcagagg
      601 aggaagtcac cagttccaat cattacccat cccgatatct tcgcccccaa gctgaaggtc
      661 aggccttttt taaagtacat aggtgccccc ttcacccggg accagctgga ggctgcaggg
      721 gcgataatgc tcccctgcag gagcccggta acgctggctc cgggggtcat gaccaccggc
      781 gaggtgcccc gggtggaggc cttcgagaca gtggaaggat tctggaccat gaaaaatgac
      841 cagaatactc ctgacgccat gcctgatgat caatcactag ccatacatct gcctggaaag
      901 gggctggttg tgatcagcgg ctgcgcccat agcggcatca tcaacaccat aagatatgcc
      961 cagaggataa cgggagagga gaggctgtat gcggtgattg gcgggttcca cctcatggat
     1021 gcagacaata agcgcataga tgccactgcc agggcccttc tcgatctgga cccggcaatt
     1081 gtccggccgg gtcactgcac cggtcagaag gccctctccc ggctgcagga tatactggga
     1141 gagagctgcc agcctctgac cgtgggagat tcaatcgagt tttagatata aaggagtgtg
     1201 aaacagttga tagcttttgg gcctgtgcct tcccgcaggc tgggacgatc cctggggatc
     1261 aacaacattc ctgccaagat ctgcagctat tcctgcgcct actgccaggt gggcaggact
     1321 ctgcagatgt cggcagagcg ccgctcctac tatctacccc agcagatctt cgaccaggtg
     1381 gcccagaagg tggcagaggc aagagatcta ggcctgacta tagattactt atcttttgtt
     1441 cctgacggcg agcccacatt ggacagcaac ctgggcgaag agatcgatat gctcaaaaca
     1501 ctcggcataa aaatagcggt gatcagcaat gcctccctga tctgggatga aaaggttcga
     1561 gaggatctat tcaaagcgga ctgggtgtcg gtcaaggtgg attctctgga ggataaagcc
     1621 tggcggcgaa tagaccggcc ccaccgcagt ctcaggctcg atacgatcct tcagggaatc
     1681 aaagatttcg cccgggaata tagtggcatc ctggccacgg agagcatgct cttagccggc
     1741 ataaacgatt gtaaggccag cctcctggcc ctagctgagt tcctggaggg gatcgatcct
     1801 gaagtatcct atatctccgt tcccatccgt cctccaacag aatcatgggt acagatgccc
     1861 tccccggaga acctgtgcct ggcttatcag atattcagcc agaggctgaa gagggtcgag
     1921 ctcctggtcg gatatgaggg agacagcttc gacttcaccg gcgaagctaa agaggatatc
     1981 ctggcgatcg cctccgttca ccccatgaga gaagctgcct tgagaagatt tttagaaaaa
     2041 tctggaaata gctgggatat aatagaagag ctgctggaaa aaaaggagct cctccgaatt
     2101 gaataccagg gcaatagctt ctacttaagg cgccctgaga gaccggataa gataggagcc
     2161 gaataagagt gttgcgggat attttgcgaa gcccatcctg ccccaaaatc cgctgtgaga
     2221 tcccgatcaa ggaatctcga taagcgatcc tgaccggtga tcttgggagc ggaattctgt
     2281 tcagtcttat cccatctggg caaatttctt gcccataatt tttggcctat tcgatctatg
     2341 cctggcggct tccctgtatg gttcccgacc aggcccctcc ctgagcatca taagcattga
     2401 agctgccgga gagattatct ttgctcatgg ttagagtgca ccggtagagc atcatgttct
     2461 gcatatcgac catatccaga tagagcacat ccttcgccgt ccatccgctg gcagacaggt
     2521 cttttgtcgt tccgaacgag gtgagcatgc cccggccaaa gactgcatcc ttgttctgca
     2581 cgagcgtaag atcaaccgtg cccggcaagc tgccatatag attcagcctc cagttgcccc
     2641 gcagatcctg agtgatctga acgaagggac tcaaatactc ccggccgctg atcttatcca
     2701 ggtactccgc cgaatgctgt ggatcgatcg gacgcatcaa cgggtcaatg ctcagtggat
     2761 cggcatttgg atcgatatag gtcttaacat tatcctgacc ggcgatggct ctcgactcac
     2821 cgggcatgag gaagatatcg cttcccagtg gatcgtaagc ctgagcttgc acgaagaagc
     2881 tggtcatcag atagatcatc aagcccccaa gggctacagt atttttggcg gacatttttt
     2941 tataaccccc aatttagttt cttcatctta agaaataaaa aattcgcctt ctaaagctgc
     3001 ctctccaggc ccttccacat atcttcgatc gcctcggcca ccggtcctcc gatctccacc
     3061 acagccagag ctttgaccat agcatcaatg accgaagtat gatagggcag ccttcccgcg
     3121 atcttcaccc ccctcttccc gcacagttcc tcgatctgcc tcgcagcttc ggggttgagg
     3181 tcatacttgt tgatgcagac cgcggtcttt atgccgaagt ggccggccac atccaggagc
     3241 ctcaccagat cgtgctcccc ggtgacggtg ggctcggtca ccaccaacgc cagatcgact
     3301 ccggtcaatg atgcgatcac cggacagcct atgcccggag agccatcta
//
LOCUS       DAOSGP010000146         3348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_26555_length_3348_cov_14.904950, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000146 DAOSGP010000000
VERSION     DAOSGP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26555_length_3348_cov_14.904950"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PLV49_05000"
     CDS             complement(<1..867)
                     /locus_tag="PLV49_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF166 domain-containing protein"
                     /protein_id="HOG98237.1"
                     /translation="MHDRREGSKGYWNGERMKLAVYYSGDFGSRVVGNLVNYSGFCIS
                     CADACTECRNVAPDLAKDIVALVEMPDPSTYGDFIDDVEPLLPQNIPKADLVIVINVH
                     PDILYGLLPKFKEAGVKAIIGGSESPKEMPLGQRRQVEEKAAELGMEAAFAKPFCALA
                     PDPNKPVIAQFLKEARIGNPVIEFSVQGSREGKDVIMGANVVRSAPCGSTWFVAKKML
                     GLETDQPDLRERISEAHHSYPCTGSMERDAELGDTVLHVGGYIIRDAVEGGLKKASKL
                     PRSKLPKPGSSIV"
     gene            complement(816..1610)
                     /locus_tag="PLV49_05005"
     CDS             complement(816..1610)
                     /locus_tag="PLV49_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG98238.1"
                     /translation="MGVAMVKRNIIKIDESLCNGCGSCAIACSEGAIEMVDGKARVAR
                     ESFCDGLGACIGECPTGALTIEWRDAEAFDEKAALEFQQQSKAEGNSPAEKRAEQEGE
                     SGPIAPCDLPIHQSQRSSRGRGEGPGQHLNKLGSSSQLSSWPIQMRLAHTNAPYFKDA
                     SLLIAADCSAFACPVISEFIRKRVVLIGCPKLDQSEPFISKLAEILSSNEIRDIAVLR
                     MEVPCCYHIINLVEQAMKRSGKNIPLDQFICMIDGKVVKDTGMVRG"
     gene            complement(1615..2925)
                     /gene="hcp"
                     /locus_tag="PLV49_05010"
     CDS             complement(1615..2925)
                     /gene="hcp"
                     /locus_tag="PLV49_05010"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOG98239.1"
                     /translation="MAATEKLDMFCYQCSQTARGTGCTLKGVCGKEPIVARLQDNLLF
                     AIKGISAYLYHARELGYSDPEVDAFIEKGFFSTLTNVNFDPEEFLNLAVKAGEMNIRT
                     MKLLKRAHIEKFGEPTPTKVRTGTVKGHGIIITGHDMNALDKLLQQVEGSDVFVYTHS
                     EMLPAHGYPGLRKYKNLAGNLGKAWFDQKKLFAQYPMALLGTSNCFLPPREEYIDRMF
                     STGPVYLPGVKHIDGYDYSEVIARARELPELPDVPGEYELTTGFSTSVLLSHAAKIKE
                     LVEKGKIKRFFLVGGCDAPLKKSDYYREFVQKLPQDVVVLTLACGKFRFNDLDLGDIE
                     GIPRLIDLGQCNDAIVALEVAGALADLFGVGVNDLPLTLVISWMEQKAVAILWSLLAL
                     GIKGIWLGPIVPGWINDDMLKILVDQYDIRLISTPDEDIKKMMG"
     gene            2998..>3348
                     /locus_tag="PLV49_05015"
     CDS             2998..>3348
                     /locus_tag="PLV49_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG98240.1"
                     /translation="MQEGCTVNQTVKYIARKWTMLILLELYKGKGHTRRFSELKGCLA
                     GITQKVLSVRLKELEREGLVEKKMDSEAFPLKSEYTLTESGLEMIEVIKSIKLWALRW
                     KIKNIECGSQDCGQC"
BASE COUNT          699 a          958 c          904 g          787 t
ORIGIN      
        1 tacaattgag ctgcctggct tgggcagctt ggatctaggc agcttgcttg ccttcttcag
       61 ccccccttcc actgcatcgc gtatgatata gccgcccacg tgcagcacag tgtctccaag
      121 ctctgcatct ctctccatag agccggtgca gggatatgaa tggtgagcct cggatatcct
      181 ctctctcagg tcgggctgat ctgtctccag gcccagcatc ttcttggcca caaaccaggt
      241 ggatccgcag ggagcgcttc gcacgacatt ggcccccatg attacatcct ttccctctct
      301 gctgccttga acggaaaact ctatcactgg gttcccaatt ctcgcctctt tcaggaactg
      361 agcgatgacg ggcttattgg gatcgggagc gagagcgcaa aacggcttgg caaaggccgc
      421 ctccattccc agctccgcag ccttctcctc cacctgtctt ctctgcccca aaggcatctc
      481 cttcggagat tccgatccgc cgatgatcgc tttgacccca gcctctttga acttgggaag
      541 caggccgtag aggatatcgg gatggacatt gatcacaatg accagatccg ccttcggtat
      601 attttggggg agcaagggct ctacatcatc tatgaaatcc ccatatgtcg agggatcggg
      661 catctccacc aatgccacaa tatcttttgc caggtccggg gcaacattcc ggcattcggt
      721 gcaggcgtct gcacaggaga tgcagaagcc tgagtagttc accaggttcc caaccacccg
      781 gctgccgaag tcgccactgt agtataccgc cagcttcatc ctctcaccat tccagtatcc
      841 tttactacct tcccgtcgat catgcatatg aactgatcga gggggatatt ctttcctgac
      901 ctcttcattg cctgctccac caggttaatg atatggtagc agcagggaac ctccatcctg
      961 aggacggcta tatcccggat ctcatttgag ctgaggatct ccgccaattt agagatgaag
     1021 ggctcgctct gatccagctt cgggcagccg atgagaacca ccctttttcg gatgaactcg
     1081 gagatgacgg gacaggcaaa tgccgagcag tcagcggcta tcagcagact ggcgtccttg
     1141 aaataggggg cattggtatg ggccagccgc atctgtatag gccagctgga gagctgggag
     1201 gaggagccga gcttgtttag atgctgtcct ggtccctctc ctctccccct actcgatctc
     1261 tgtgattgat gaattgggag gtcgcagggg gctattggtc ccgattcgcc ctcttgttcc
     1321 gccctctttt cggcggggct gttgccctct gctttggatt gctgctgaaa ttccagggcc
     1381 gccttctcat cgaatgcttc tgcatctctc cattcgatgg tcagagctcc agtgggacac
     1441 tcgccaatgc aggctcccag gccgtcgcaa aacgattctc ttgctacccg cgctttgcca
     1501 tccaccattt ctatcgctcc ctctgagcag gcgatagcgc agctgccgca gccattgcag
     1561 agtgactcat cgatcttgat gatatttctc tttaccattg cgactcccaa tcatctaccc
     1621 catcatcttc ttaatgtcct catccggggt gctgatcagc cggatgtcgt actggtccac
     1681 caggatcttg agcatatcat cattgatcca tccggggacg atggggccca accagatccc
     1741 tttgattccc aatgccaata aggaccagag aatggccacc gccttctgct ccatccagct
     1801 tatcaccagt gtcaagggca ggtcattgac tcccactcca aacaggtcgg ccagagcccc
     1861 ggccacctcc agggcgacga tggcatcgtt gcactgaccc aggtcgatca gacgggggat
     1921 gccctctatg tcccctaaat ccaggtcatt gaagcggaac ttgccgcagg ctaaggtgag
     1981 gaccactaca tcctggggca gcttttgcac gaactcccgg tagtagtcgg acttcttcag
     2041 gggagcatcg cagccgccca ctaagaagaa cctcttgatc ttgcccttct ccaccagttc
     2101 cttgatcttg gcggcgtggg agaggagaac ggaggtggaa aagccggtgg tcagctcata
     2161 ttctcccggg acgtctggga gctccggaag ctctcgagct cgagcgataa cctcggagta
     2221 gtcgtatcca tcgatgtgct tgaccccagg caggtagacc ggcccggtgg agaacatgcg
     2281 gtcgatgtac tcctcacgcg gaggaaggaa gcagttggag gtgcctaata gggccatggg
     2341 atactgggcg aagagcttct tttggtcaaa ccaggctttg cccaggttgc ctgccaggtt
     2401 cttgtacttt ctcaagcccg gatagccgtg ggcggggagc atttcggagt gggtgtagac
     2461 gaagacgtct gagccctcca cctgctgcaa gagcttatcc agggcgttca tgtcgtggcc
     2521 ggtgataatg atgccgtggc ccttgaccgt tcccgtcctc accttggtgg gcgtgggctc
     2581 gccgaacttc tcgatgtgcg cccgcttgag cagcttcatg gtcctgatat tcatctcccc
     2641 cgctttcact gccaggttga ggaactcctc cgggtcgaag ttcacattgg tcagcgtgga
     2701 gaaaaagccc ttctctatga atgcgtccac ctccgggtcg ctgtagccca gctcccgggc
     2761 atgatagaga taggcgctga tgcccttgat ggcgaagagc aggttatcct gcagccgggc
     2821 gacgatcggt tcctttccac atactccttt cagtgtgcag ccggtgcccc gggcggtctg
     2881 ggagcactgg tagcagaaca tgtccaattt ttcagtagct gccatttttt attgcctcac
     2941 aaccagtata ctattgatac caaagtatat aacctcgaaa gtttccctga gtataccatg
     3001 caggaaggat gcacagtaaa ccagactgtg aagtatattg ccagaaaatg gaccatgcta
     3061 atactcttgg agctctacaa gggaaagggg cacaccaggc ggttctctga gttgaagggc
     3121 tgtctggcag ggatcaccca gaaggtccta tccgttcggc tcaaggaatt ggagcgggag
     3181 gggctggtgg agaagaagat ggacagcgag gcctttcccc tgaagagcga gtacaccctc
     3241 accgagagcg gcttagagat gatcgaggtc atcaagagca taaagctgtg ggccttgcgc
     3301 tggaagataa agaacatcga gtgcggcagc caggattgcg ggcagtgc
//
LOCUS       DAOSGP010000147         3341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_26636_length_3341_cov_12.632684, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000147 DAOSGP010000000
VERSION     DAOSGP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26636_length_3341_cov_12.632684"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PLV49_05020"
     CDS             complement(<1..773)
                     /locus_tag="PLV49_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG98241.1"
                     /translation="MNPGVITEGISLSYNSRLVLGDVSLQLRKGAIVTLLGPNGCGKT
                     TLLKIINGLLRPDSGKVYVDGYDVSRMGQTDMARLMGHVPQTQRSSFPFTALDIVLTG
                     RMPHISALSQPGPRDLEKARQAMDMVGASHLSSRPYTQISGGERQLVMIARALAQEPS
                     FLLLDEPTSYLDFKNQHQVLKMISRIARDQKVTVVMTLHDPNHALMFSDELVLLRKLT
                     EKECGDEQKFKGESNCNHQNVVAAGRPAEVMTPENIFEA"
     gene            complement(776..1822)
                     /locus_tag="PLV49_05025"
     CDS             complement(776..1822)
                     /locus_tag="PLV49_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG98242.1"
                     /translation="MPSQTEKMGMARERITGRSLLYYLFLSLPLFAFFSSIFIGPFHI
                     NPVQLVDLMASKILGYATDYPDVYSTVIFQVRLPRLLLGMMVGASLSVSGAAFQGIFR
                     NPLVSPYILGLSSGAAFGAALSLAFLPGVSIQYAAFFFSLLAVALTYFMATTNRSTPI
                     VSLVLAGVIVSAVFDSLLAIIQIAVSERALQSIVFWIMGSLGTASWSKIYGAFPLFAL
                     GCTVMLLLRWRLNVLALGDEEARSVGLNPEKYKIIFIVSASLAASSAVAVAGIIGLVG
                     LVVPHMLRMIFGPDHRTLIPLSLTFGASFLIVVDDFARAGLGFEVPVGVITTLVGAPL
                     FAYLLRTTRSGGWE"
     gene            complement(1834..2877)
                     /locus_tag="PLV49_05030"
     CDS             complement(1834..2877)
                     /locus_tag="PLV49_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG98243.1"
                     /translation="MKSSTYFLLAVALCLLLAVPASYANETVLIEDDLGRSINIGSPS
                     ERVVFTMENALKTYYAVGDPDNVVALKDDKWMRRLMEDVFPVVDPNFEDKILINITGD
                     QLGLESLAKVDPDLVVLWASSPDDPNILAINDTLKVPVFAIYVTSLDDVYRQVDTMGI
                     ISGNTDRASEVKEIMEGYVELTTNVTDQIQESDRPGVFWMWTDIYGTAGVDSGINDLI
                     DMAGGNNVMKLAEEEAQNLEHPVINQETLIKLNPDVIYMWYNEDLDPEDIINGEDFQG
                     LRDINAVKNRRVYEISNPYLFDAFSPRMPLALLHVARDLHHDKFQDVDMNQTIDQYNV
                     DIWGVHYPSMVKA"
BASE COUNT          841 a          875 c          825 g          800 t
ORIGIN      
        1 taggcctcaa agatgttctc cggggtcatg acttctgcgg gccttcctgc tgctaccaca
       61 ttctgatgat tgcaattgga ctcgcctttg aatttctgct catccccaca ttctttttct
      121 gtcagctttc gcagcagtac cagctcatcc gagaacatca gggcatgatt gggatcatgc
      181 agagtcataa ccaccgtcac cttctgatcc cttgcgatcc gggatatcat cttgaggact
      241 tgatgctgat ttttgaagtc cagataagag gtgggctcgt ccagcaatag aaaagagggc
      301 tcctgagcca gagccctggc gatcatcacc agctgcctct cccctccact gatttgagtg
      361 taaggcctgc tggatagatg agaagctccc accatatcca tggcctgacg agctttttcc
      421 agatctctcg gtcctggctg ggatagggcg gagatatggg gcatcctccc ggtaagtacg
      481 atatccaggg ccgtaaaggg aaatgatgac ctctgagtct ggggcacatg tcccatcagc
      541 cgtgccatat cggtctgccc catccgggag acatcatatc catccacata gacctttcca
      601 gaatcaggac gaaggagtcc attgatgatc ttcaatagag ttgtctttcc gcagccattg
      661 ggacccagaa gggttacgat ggctcccttt ctcaattgca ggctaacatc gcccaagacc
      721 agacgactat tgtagcttaa agagatgccc tctgtaatca cgcctggatt catgttcact
      781 cccaaccacc cgatcgcgtt gtgcgaagca gataagcgaa gaggggcgct cctactaatg
      841 tggtgatcac cccgaccggc acttcgaatc ccagccccgc ccgggcgaag tcgtcaacca
      901 caatcagaaa agaggcgcca aatgtcagag ataggggaat tagtgtcctg tgatccggcc
      961 caaagatcat gcgcagcata tggggaacga ccagccccac caggccgatg atcccggcca
     1021 cggcaaccgc agaagaggcg gcaagagatg ctgagacgat gaaaatgatc ttgtatttct
     1081 ctggattcag accaactgag cgcgcctcct catcacccaa agcgaggaca ttcagcctcc
     1141 agcgcaatag cagcatgacg gtgcatccca gtgcgaagag cggaaaggcg ccatatatct
     1201 tcgaccagct cgctgtacca aggctgccca taatccagaa gactatgctc tgcagggccc
     1261 tctcgcttac ggcgatctga attatagcca ggagggagtc aaacaccgca gagactatca
     1321 cccccgccag gacaagggag actatgggag tgctccggtt ggtcgttgcc atgaaatagg
     1381 tcagagccac tgccagcaga ctgaagaaga aagcagcgta ttgaatggac actcccggca
     1441 aaaaggctaa tgagagggca gctccaaagg ccgctcctga ggataggccg aggatgtaag
     1501 ggctaacaag cggatttctg aaaatgccct ggaatgccgc cccggagacc gagaggctgg
     1561 ctcctaccat catgcccaaa agcagtcgcg ggaggcgaac ctgaaagatc acagtgctgt
     1621 agacgtccgg gtagtctgtg gcatagccca atatctttga ggccatcaga tcgaccagct
     1681 gcactggatt tatgtgaaaa gggcctatga aaatggatga gaagaaggca aaaaggggaa
     1741 ggctcaaaaa tagataatag aggagcgatc tgcccgtgat tctctcacgc gccatgccca
     1801 tcttctcagt ctgagatggc atcaaattcc atctcaggct ttaaccatgc tgggataatg
     1861 cacgccccag atatccacgt tgtattgatc gatagtctgg ttcatgtcca cgtcctggaa
     1921 cttatcgtga tgcagatccc tggcaacatg cagcaaagcc agaggcattc ggggggagaa
     1981 ggcatcaaat agatagggat tgctgatctc atagacccgg cggttcttta cagcatttat
     2041 gtccctcaag ccctggaaat cctccccatt gatgatgtcc tcaggatcca ggtcctcatt
     2101 gtaccacata tagatgacat cggggttgag cttgatcaga gtctcctggt tgattactgg
     2161 atgctctaga ttctgcgcct cctcttccgc cagcttcatg acattgttgc ctcctgccat
     2221 atcgatcagg tcgtttatgc cgctatcgac ccctgctgtg ccatagatat ccgtccacat
     2281 ccagaaaacc cccggcctat cgctctcctg gatctgatcc gtgacgttgg tggtcaattc
     2341 aacgtatcct tccattatct ccttaacctc ggatgcgcga tcggtgtttc ctgagatgat
     2401 gcccattgta tccacctggc ggtagacatc atcaagagag gttacgtaga tggcaaagac
     2461 gggcactttc agtgtatcat tgatggccag gatgttcggg tcatcgggag aggaggccca
     2521 caggaccacc agatcgggat ctaccttggc cagggattcg aggcccagct ggtcgcccgt
     2581 gatgttgatc agtatcttat cctcgaagtt gggatcgact acgggaaata catcttccat
     2641 tagccttcgc atccatttgt catctttaag ggcgaccaca ttatctggat ctcctacggc
     2701 ataataggtc ttgagggcat tctccatggt gaagaccact ctctctgatg gggagccgat
     2761 gttaatgctt ctccccagat cgtcctctat aagaacggtc tcattagcat agctggctgg
     2821 cactgccaga agcaggcaga gggctacagc tagcaagaaa taggtcgaac tcttcatact
     2881 attgcctcca acagatcgat ttcgtacttg attgcgtatt ctttaactta tcctgcactt
     2941 tccaaattta tgattgttgc gtacataata tgaccaggca gatgatatta tgatactaga
     3001 ctccgcaatg atatcactat ttataaagcc ggcggtagca ggtgaaattt ttgaaactac
     3061 agaacttatc caataaaaca aaggctcttc gctatttggt atgggtgaat agaatagcaa
     3121 attctctgca tctttgcgcc actgtggtga accgggatgc tttgacccaa ccatagagat
     3181 ggagtagtca tccttttcat cataatgaga agcatccgag accttgcctt tccttaagct
     3241 gctctacgtc gcccggcata ttggccggca aaggttttat agggcagata tcattgctca
     3301 atctatgaca tatgaaacct ctgtggggaa tcgtcttcta c
//
LOCUS       DAOSGP010000148         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_26744_length_3331_cov_16.049145, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000148 DAOSGP010000000
VERSION     DAOSGP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26744_length_3331_cov_16.049145"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /gene="rqcH"
                     /locus_tag="PLV49_05035"
     CDS             <1..1512
                     /gene="rqcH"
                     /locus_tag="PLV49_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HOG98244.1"
                     /translation="KMLYRGRKMAAGEPYLYHPGQLDPRTIARDELALWLASSGQDLV
                     RSLVRGLNMGGTYGEEVCLVAGVDKNKEAIDLDPGEIDRVHQALSEVFLNETLDPHIV
                     IEDGAPIDVLPRPLKLYSGLEKKRFVTFSEALDAFFVEREKETTRQNPLEHRIELQRK
                     AIEDFRAQEAELVRKGELIYQLYGSVEQILTLMNDARARGFSYNQIWERISGSGLPQA
                     KTILSLDGRGEMRVLLEEELELNASLTVPQNAQRYYDKAKDMVRKAKGAQSALAITEE
                     LKAGKVAPKKTRVVSSYYRRRKPKWYERFRWFYSSDRFLVLGGRDADSNEEIYGKYLE
                     RRDLAMHTDAPGAPLTVIKTEGKEVPQSTLQEAAGFAVSYSSLWKSGLAAADCYLVKG
                     DQVSKTPESGEFLKKGAFVIRGERRYFRDVPLGIALGIRDGVLIGGPVSASKPGSDPV
                     VEIEPGELNPDDLAKRIYRQFSLKVEDRAFLKSVASVDQIAQFLPPGNSRMKE"
     gene            complement(1568..2464)
                     /locus_tag="PLV49_05040"
     CDS             complement(1568..2464)
                     /locus_tag="PLV49_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOG98245.1"
                     /translation="MSGNQARANEKKEASGKGGKKSSQARSLRETVQFYMIDFKTPLG
                     KAIDIFIILLNLLVVLLFVIKTYPLSAGMQEILWRTEVAAVSLFIIEYLLRLYGAPDR
                     WAYVKDGYSIIDIVAIMPTLVLMVLPATLFTHDIRFIQTIRVMAAFRIFRFLRFMAKD
                     HLLFGMISPGMINVARLVASIFIILFVYSGLFYFVEEPVNPDVQNFGDAFYFVVVAVS
                     TVGFGDIVPSSSAGRLVTVAMIISGIIFIPFQAARIFRAWLQASSQKRMVICTNCGLD
                     RHDVDAEYCKKCGEPLPETGEG"
     gene            2559..>3331
                     /locus_tag="PLV49_05045"
     CDS             2559..>3331
                     /locus_tag="PLV49_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOG98246.1"
                     /translation="MIEFSDVLQAANLLKDRVIRTPLVYSPTFSRMAQAEVYLKLESL
                     QMGGSFKVRGATYKLQSNLKKIQPGVIAASVGNHAQGVALAASAAGVPATIIMPLWAS
                     ISKQEATRAYGAEVRLMGKSLVESIDIGRRMAQETNRIFIHPYDDEEVISGQGTVGLE
                     ILEDLPDTDFIIVPVGGGGLIAGIALAAKAIRPEARVVGVQAASCPSARKALELGRPA
                     EVQAEERGSIADAIMVTQVGEAPFPLLQKLVDDIVLVDE"
BASE COUNT          783 a          859 c         1019 g          670 t
ORIGIN      
        1 aagatgctct accggggaag gaagatggcc gccggggagc cctacctcta ccaccccggc
       61 cagctcgatc caaggaccat agcccgggat gagctggccc tctggcttgc ctccagcggc
      121 caagacctag tgagatctct ggtgcgcggt ctgaacatgg gcgggacata cggcgaggag
      181 gtctgcctgg tggccggagt ggacaagaac aaggaggcga tcgacctgga ccctggagag
      241 atcgaccgtg tgcatcaggc attaagcgaa gtgttcctga atgaaaccct cgatcctcat
      301 attgtcatcg aagatggagc ccccatagat gttctgcccc gccctctgaa gctctattcc
      361 ggtctggaga aaaaaaggtt tgtcaccttc agcgaagctc tggatgcctt ctttgtggaa
      421 agggagaaag agacgacccg gcagaatcct ttggagcaca ggatagagct gcagcgcaag
      481 gccattgagg attttcgagc ccaggaggcg gaactggttc gaaagggcga gctgatctac
      541 cagctctacg gtagtgtcga gcagatactc accttaatga acgatgcccg ggcgcggggc
      601 ttctcctaca accagatatg ggagaggatc agcggctcgg gccttcccca ggcgaagacg
      661 atcctctctt tggacggcag gggagagatg cgggtcttgc tggaggagga actggagttg
      721 aatgccagct tgacagtccc ccagaatgcc cagaggtact atgataaggc caaggatatg
      781 gtccgcaagg ccaaaggggc acagagcgct ctggctatta ccgaggagct gaaagcggga
      841 aaggtcgctc ccaagaagac ccgcgtcgtg tcttcatatt acaggaggag aaagcccaag
      901 tggtatgaga ggttccgctg gttttactcc tccgaccgct ttctggtcct ggggggaagg
      961 gatgcggata gcaatgagga gatttatggc aaatacctgg agagaaggga cctagccatg
     1021 cataccgacg ctccaggcgc tcccctgacg gtgattaaga ccgagggcaa agaggtgccc
     1081 cagagcactc tccaggaggc ggccgggttt gcggtgagct actcctccct ctggaagagc
     1141 gggcttgctg ctgcagactg ctatctggtg aagggtgacc aggtgagcaa gaccccggag
     1201 tcgggggagt ttttgaagaa gggtgccttt gtcatccgtg gagagcggag gtacttcagg
     1261 gatgtgccac tgggaattgc tctggggata agagatggag ttcttattgg cggtcccgta
     1321 tcggcgagca agcccgggtc ggatccggtg gtggagatcg agccgggaga gcttaatcct
     1381 gacgacctgg caaagaggat ctaccggcag ttctctttaa aggtggagga cagagccttc
     1441 ttgaagtccg tggcgtcggt ggatcagatc gcgcagttcc tgcctccggg aaattcgaga
     1501 atgaaggaat gattgagaca aatccaaaac aaaaaggcac tcctgaaggc aaatgctctc
     1561 gaaccagcta gccttctcct gtctccggca gaggctcgcc acattttttg cagtactctg
     1621 catcgacgtc atgcctgtcc agaccgcagt tggtgcatat caccatcctt ttctggctag
     1681 aagcttgcag ccaggcgcga aatatcctcg ctgcctggaa ggggatgaag atgatcccgg
     1741 agattatcat ggccaccgtg accagccggc cggcagagga tgagggaacg atatcaccga
     1801 agccgacggt ggagacggca accaccacga agtaaaaggc atctccgaag ttctgcacat
     1861 caggattgac cggctcctcc acaaagtaga agagtcctga atatacaaag aggattatga
     1921 atatgctcgc caccagccgg gcgacgttaa tcatccccgg gctgatcatc ccgaagagca
     1981 gatgatcttt tgccataaag cggaggaacc tgaatatcct gaatgccgcc atcactctta
     2041 tggtctgaat gaaccgaata tcgtgggtga agagggtggc gggcaggacc atcaaaacca
     2101 gggtgggcat tatagccacg atatcgatga tgctgtagcc atctttaaca taggcccagc
     2161 ggtcgggagc gccatagagc cgcagcagat actcgattat aaacaggctc actgccgcta
     2221 cctcagtcct ccagaggatc tcctgcatgc ccgctgaaag gggataggtc ttgatgacga
     2281 agagcaggac caccagcaga ttgagcagga tgatgaagat gtctatggcc ttacccaagg
     2341 gggttttgaa gtctatcatg tagaactgga cggtctcccg caagctcctg gcctgagaag
     2401 atttcttgcc cccctttccc gaagcctcct ttttctcatt ggctcttgcc tgattgccgg
     2461 acatctgaat gcttattgca tctttcaggg gataaaacca tccgaatcag ctcaggaaat
     2521 gattaagtct tcagacagca gtgtgttatt atgatattat gatcgaattc tctgatgtct
     2581 tgcaggcggc aaacctcctc aaggacaggg tcatccgcac ccctctggtc tactctccta
     2641 cgttcagcag gatggctcag gccgaggtct acctgaagct ggagagcttg cagatgggcg
     2701 gctcgttcaa ggtcagaggg gccacctaca agctccagtc caatctgaaa aagatccaac
     2761 cgggggttat agccgcctct gtgggaaacc atgcacaggg ggtggccctg gccgccagtg
     2821 cagccggtgt ccctgctacg atcatcatgc ccctctgggc ctccatctct aagcaagagg
     2881 ccacccgagc ttatggggca gaggtgaggc tgatgggcaa gagcctcgtc gagagcatag
     2941 atatcggcag gcgcatggcc caggagacga acaggatctt cattcatccg tatgacgacg
     3001 aggaggtcat atccggccag ggcacagttg gcctggagat cctggaggac ctgcccgata
     3061 cagacttcat aatagtcccg gtgggcggag ggggattgat cgcaggaatt gccctggctg
     3121 ccaaggccat ccggccggag gcaagggtgg tgggggtgca ggcggcatcc tgcccctcgg
     3181 ccagaaaggc cctggagctt ggaaggccgg cggaggtcca ggctgaggag cggggctcga
     3241 tcgccgatgc catcatggtc acccaggtgg gcgaggcccc attcccgttg ctgcagaagc
     3301 tggtggatga tatcgttctg gttgatgagg a
//
LOCUS       DAOSGP010000149         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_27025_length_3306_cov_19.235620, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000149 DAOSGP010000000
VERSION     DAOSGP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27025_length_3306_cov_19.235620"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1439
                     /locus_tag="PLV49_05050"
     CDS             <1..>1439
                     /locus_tag="PLV49_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HOG98247.1"
                     /translation="SQMEEHRLVRRDGGILQRTRKAREYYIENLSMIPDEKRYNVYDI
                     VGRRSVGTLDEAFVVGFAQPGATFVTKGEIWEITEIAEEEIKVVPIQRSGEIPSWTGE
                     EIPVPFDVAQEVGRIRRKISEMLGSGKAAEDAEEWLQSCYPVEDEATRELVDLIMRQR
                     EKNHPVPNESLVVVEGGGEGAIINCCFGHKTNDTLGRVITSILSARFGSSVALQIDPY
                     RIELTLPKALLAEEIEKLIEDLDPDYVQPILEMTLKNTSLLRWKMIHVARKFGALSRD
                     VDYQRVSMAKLLAVFEGTPMYREALREIYHDRLDIERTRMVLVRIRDGSMAMTTSSLS
                     PIGTSGRGGGKDVTSPENADAAVIKLLKNRIMNDRVLLFCVNCKKWKSMRQVERVPDR
                     PECPLCGSRMVAALKPWEEDEIKVVRKRDKKTSEEKRRTKRVFRNANLVLSYGKTAAI
                     ALASRGLGPETAARVIGKLQKDEIEFYRD"
     assembly_gap    1440..1539
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1922..3253)
                     /locus_tag="PLV49_05055"
     CDS             complement(1922..3253)
                     /locus_tag="PLV49_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOG98248.1"
                     /translation="MNYWQPKLELMERSELEELQLKRLKSIAEKVYNSVPFYRNKFKE
                     AGVSPDDIKSLNDLSKLPTTRKQNLRENYPFGLFAVPLDEVVRVHASSGTTGKPTVVG
                     YTARDIETWSDLMARDFVMAGVRKGDIFQNAVNYGFFTGGLGVHYGIERMGAMAVPSG
                     VGNTSRQLEIMMDFGVTVLHCTPSYALYLAETAKANGVVDKLKLRIGCFGAEPWSDEA
                     RKELEEALNIKAYDSYGLSEMMGPGVAFECQEQDGLHIWEDHFLIEILDNNEQPCAPG
                     EPGELVVTSLTKEAMPLIRYHTGDVTYIMEEKCSCGRTSRKLHRFLGRADDMLIVRGI
                     NVFPSQIEDVLVSIPEIGNYFQVVVDRKKHGLDEISIQVELKDEAFTGELADLARIRK
                     ITEDKLKSVLNVRSRIELVEKGSIPRTAGKSKKVVDLRDVYAKDERAKGTS"
BASE COUNT          766 a          833 c          837 g          770 t
ORIGIN      
        1 tctcagatgg aggagcacag actggtcagg cgagatggag gcatcctgca gaggactcgc
       61 aaggcacggg agtactatat cgagaacctc tccatgatcc cggatgagaa gaggtacaat
      121 gtctacgaca tagtgggacg cagatcggtg ggaaccctgg atgaagcatt tgtggttggc
      181 ttcgcccagc ccggtgccac attcgtgacc aagggagaga tctgggagat aaccgagatt
      241 gctgaggagg agattaaggt ggttcccatt cagcgaagcg gagagattcc cagctggaca
      301 ggagaggaga ttcccgtccc ctttgatgtg gcccaggagg tgggccggat ccggaggaag
      361 atcagcgaga tgctcggatc gggcaaggct gcggaagatg ctgaagagtg gctccagagt
      421 tgctatccgg tggaagatga ggcgacaaga gagctggttg atctcataat gagacagagg
      481 gaaaagaatc atcctgtacc aaacgaatct ctggtggtag tggaaggcgg tggggaggga
      541 gcgatcataa actgctgctt cggccataaa acaaatgaca ctcttgggag ggttataacc
      601 tccatcctat ccgcccgctt tgggagcagc gtggccttgc agatcgatcc ttacagaatt
      661 gagctgaccc ttcctaaagc gttgctggcg gaagagatcg agaagctgat cgaggatctt
      721 gatccggact atgttcagcc catcctggag atgaccctga agaacacctc cctccttcgc
      781 tggaagatga tccatgtggc ccgcaagttc ggtgccctat ccagggatgt tgactatcag
      841 agagtgagca tggcaaagct gctggcagta ttcgagggca cccccatgta tcgagaggct
      901 ttgcgggaga tctatcacga tcgcctggat atcgaacgga ccaggatggt tctggtgagg
      961 atcagggatg gctcgatggc catgaccacc agcagcctct cgcctattgg gaccagtggc
     1021 cggggaggcg gaaaggatgt aacctctccg gagaatgcgg atgctgcagt gatcaaactc
     1081 ctcaaaaaca ggattatgaa cgaccgcgta ctgctatttt gcgtgaactg caagaaatgg
     1141 aagtccatgc gtcaggtcga acgggtgccc gaccgacctg agtgccctct ttgtggttcc
     1201 aggatggtgg ctgctctcaa gccctgggag gaggatgaga tcaaagtggt ccgaaagcgg
     1261 gataagaaga cctctgagga gaagcgcagg accaagaggg tctttcgcaa tgcaaacctg
     1321 gtcttaagct atggcaagac cgcagccatt gcccttgcca gccggggttt aggacctgag
     1381 accgcagcga gggtgatagg caagcttcag aaggacgaga tcgagtttta cagggacatn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ccatgatgat gtgcttcatc
     1561 ataatgcaat ctctctgatc ttcagcaccg caggtatgct caaagaattc gctttggcca
     1621 ttcttcgaat cggatttccc tttttctgca aattcaatca tgtccttcta ctcctgcagc
     1681 catatttcgg aaccggagat ttgtttgccc ttgaagccgc agcctcacga tccttatcaa
     1741 ggaacatgcc ggttctgcat atgccattat aatattcacc agttgcatta aaggcgcatt
     1801 gagatggttt atataactat taagaattat agactatata actcttccct ccggcgatat
     1861 gtatttaaaa tattgaaaaa aaaccgagga gaattagcag ggcgatttag agcttatgcg
     1921 ttcagctcgt tccctttgcc ctctcatcct tggcatagac gtcccgcagg tccacgacct
     1981 tctttgactt accagccgtt cttggaatcg accctttctc caccagttct atcctgctcc
     2041 gcacattcaa gaccgatttc agcttatcct cagtgatctt ccggatccgg gctaagtcag
     2101 ccagctcgcc ggtaaatgcc tcgtccttca gctcaacctg aatgctgatc tcatccagac
     2161 cgtgcttctt tctgtccacg accacctgga agtagtttcc aatctctgga atgctcacta
     2221 agacatcctc gatctggctg gggaagacat tgatgccccg cacaatgagc atatcatcgg
     2281 ctcttcccag gaatctatgc agctttctgc tggtgcgacc acaggagcat ttctcttcca
     2341 taatataggt cacatcgccg gtatggtatc ttatcagagg catagcctcc ttggtcagag
     2401 aggtaaccac cagctcgccg ggctcaccag gcgcacatgg ctgctcgtta ttatccagga
     2461 tctcaatcaa gaaatggtcc tcccagatat gcagaccgtc ctgctcctga cactcgaaag
     2521 ctacgcccgg ccccatcatc tcagagagac cgtaagagtc gtaggctttg atgttcaggg
     2581 cctcctccag ttctttgcgt gcctcgtccg accagggttc agctccaaag cagccgatcc
     2641 tcagcttgag tttatccacc acaccattag ctttagctgt ctctgccaaa tagagggcat
     2701 aggagggcgt gcagtgcagg acggtcaccc cgaagtccat catgatctcc agctggcgac
     2761 tagtatttcc cacaccggaa ggaacagcca tggcacccat ccgctcgatg ccatagtgaa
     2821 ctccaagccc tccggtgaag aatccgtaat tcaccgcatt ctggaatatg tctccctttc
     2881 ttacccccgc catcacaaag tctctggcca tgaggtctga ccaggtctca atgtctctgg
     2941 cggtgtagcc gaccactgtg ggctttcccg tcgttccgga agaggcatga acccgcacaa
     3001 cctcgtccag aggaacagcg aaaaggccaa aggggtagtt ctccctgaga ttttgcttgc
     3061 gagtggttgg aagctttgac aggtcattga ggctttttat atcatctgga gaaactcctg
     3121 cctccttgaa tttgttgcgg taaaatggaa cgctattgta gaccttctct gcgatgctct
     3181 tgagccgctt gagctgcagc tcttccagct cacttctttc cattaactcc aatttcggtt
     3241 gccagtaatt cattttcata ccctggtgaa tttgatcttg ctcgtatgat gctgctaatg
     3301 ctacac
//
LOCUS       DAOSGP010000150         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_27261_length_3288_cov_16.956697, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000150 DAOSGP010000000
VERSION     DAOSGP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27261_length_3288_cov_16.956697"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLV49_05060"
     CDS             <1..294
                     /locus_tag="PLV49_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOG98249.1"
                     /translation="LGNRFGGLSGRAIKPVAVRAVYDLASRLEIPVIGVGGISSWEDA
                     VEMIMAGASAIQVGTALLQGYGIFEEITSGLSAYLERKSITLDELCGMAGRCS"
     gene            291..1061
                     /locus_tag="PLV49_05065"
     CDS             291..1061
                     /locus_tag="PLV49_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOG98250.1"
                     /translation="MRPIDTIIMEVQSETPLIKTLRLDRSLDPHPGQYAMVWIRGLDE
                     VPMSFSGPDTITVQSVGEASEALNSMGEGRSIGLRGPLGKGFTLLGERILIIGGGVGT
                     APLAFLGEQAMNAGMEVTSLIGYRCCSDMIFLKRFQRLGRTIVTTDDGSSGIRGRVAA
                     GLEALDLDDYDQIYLCGPELMMWDVISRSREHAKKTQACINRYFKCAVGICGSCCLDP
                     DGLRVCVEGPVIRADRLLESEFGRYRRGPTGVKGPCRG"
     assembly_gap    1094..1193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1218..1949
                     /locus_tag="PLV49_05070"
     CDS             1218..1949
                     /locus_tag="PLV49_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOG98251.1"
                     /translation="MIVRFKKDGLVRGGSYSIEHGIVSGGEIIPLEDAELLIPCQPSK
                     IICVGLNYVEHARELKMKLPEEPIIFLKPPTAALSPGGEIIYPPSSRQVDYEGELAVV
                     IGKRGRNIPADEAEGHILGYTCFNDVTARDLQQRDTQWTRAKSFDTFAPFGPWIASID
                     PSGVDIETRVNGETVQKSNTSDLIFGVPRLIEFISGIMTLQPGDVIATGTPPGVGQLH
                     LGDLVEVEIEGIGVLKNSVVAADRS"
     gene            complement(2006..>3288)
                     /locus_tag="PLV49_05075"
     CDS             complement(2006..>3288)
                     /locus_tag="PLV49_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HOG98252.1"
                     /translation="KGGFEPGYSTLEDWILAQAIPFSLDSTELNDAIDRAMARIGDTV
                     RLLGFGEALHGKSDILLLRNRLFIRLVENHGFVAMAIESSFPRGRLVDQYIAGQGPDS
                     FDDILELGFSHGFGRLDANRELVEWMREYNSDPSHAHKLHFYGFDSPTEMLASDSPKQ
                     LIYFVLDFLGTIDPVQGEEHRQRIEPFLGQESDWDNPDALIDPAKSIGRSPNAIALRA
                     EVEDLYEELLVRRPELVAGSDPARYQEAVHYAALARQQLSYHAELAGNSLDRTSRLLG
                     IRDAMMAENLAYITSREGSQRGRVMAFAHNRHLQRGRAEWQYTDDLYSWWPAGSHLDQ
                     IMGSGYAVIGTAVGVSSSNGIGKPEESTLEARLTAAPGPALLIPTCKGQGLPADEIAS
                     LPRRSGSAKNPTYFPLGPESFTDFDWLAVLDSTA"
BASE COUNT          691 a          842 c          980 g          675 t
ORIGIN      
        1 ctgggcaacc gcttcggagg cctatccgga cgggccataa agccggtggc cgttcgcgcg
       61 gtgtacgatc ttgcctcccg tctggagatc ccggtgatcg gggtgggcgg aatatcctcc
      121 tgggaggatg cggtggagat gatcatggca ggagcctctg ctattcaggt ggggaccgcc
      181 cttttgcagg gctatggcat attcgaggag atcacatccg gcctgtcagc atacctggag
      241 aggaaatcaa taacactaga tgagctgtgc ggcatggctg ggaggtgctc atgagaccta
      301 tcgacaccat tataatggaa gtccaatccg aaacccctct gatcaagacc ctacggctgg
      361 acaggagcct ggatcctcat cccgggcagt atgccatggt ctggattcgc ggactggatg
      421 aggttcccat gagcttctct ggacctgata ccatcactgt gcaatcggtg ggtgaggcct
      481 ccgaggccct caattccatg ggcgagggaa ggagcattgg gctgcgtgga cctcttggca
      541 aaggttttac tcttctggga gaaaggattc tgatcatcgg aggcggggtg ggaactgcgc
      601 ctttggcctt cctgggcgag caggctatga atgcgggcat ggaggtcaca tcgctgatcg
      661 gctatcgctg ctgtagcgat atgatattcc tgaagaggtt ccagaggctg ggcagaacaa
      721 tcgtcaccac tgacgatggt tcaagcggga tccgcggccg ggtggccgct ggccttgagg
      781 ctctggacct tgatgattac gaccagatct atttatgcgg cccggagctg atgatgtggg
      841 atgtaatctc ccgatcacga gagcatgcca aaaaaactca ggcctgcatc aatcgctact
      901 tcaaatgcgc tgtgggcata tgcggatcat gctgcctgga tccggatggg ctgagagtct
      961 gcgtggaagg gccggtgatc agggctgatc gccttctaga gagcgagttt ggccggtacc
     1021 gccgcggacc gacaggtgtc aaggggccct gcaggggatg aaaattatag gggtaaagaa
     1081 aggcgatgaa gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatgaag
     1201 aaaggggatg aaagaggatg attgtcagat tcaagaagga cggcctcgtc cggggcggct
     1261 cgtacagcat tgagcatgga atagtatcgg gaggagagat aattcccctg gaggatgcag
     1321 agctgctcat accctgtcag ccatcaaaga taatctgcgt gggcctgaac tatgtggagc
     1381 acgcccggga attaaagatg aagctgcccg aggagcccat catctttctt aagcctccta
     1441 ctgccgcgtt gagccctggg ggcgagatca tatatcctcc ctccagccgg caggtggact
     1501 atgaagggga actagcggtg gtgataggaa agaggggcag gaatatcccg gcagatgagg
     1561 cggaaggtca tatcctggga tacacctgct ttaacgatgt tactgctcgc gatctgcagc
     1621 aaagagatac tcaatggacc cgggccaaga gctttgatac ctttgctccc ttcggcccat
     1681 ggattgcgtc gattgatccc tctggcgttg acatcgagac ccgggtgaac ggcgagaccg
     1741 tgcaaaagtc caatacctcg gacctgatat tcggcgttcc caggctcatc gagttcataa
     1801 gcggcatcat gactctgcag ccgggagatg tgatagccac tggaacgccg ccgggagtgg
     1861 gacagctgca tctgggcgat ctggtggagg tggagatcga ggggataggc gttctcaaga
     1921 actctgtggt ggctgccgac aggagctgaa gggatcgttt aactgccagc attgtccaat
     1981 tttttatcct tcccggcgca aacgctcatg ccgtcgagtc tagaaccgcc agccagtcga
     2041 agtcggtgaa gctctctggc cccaggggaa agtaggtggg attctttgcg cttccggatc
     2101 ggcgcggaag agaggcgatc tcatctgcag gaagcccctg gcctttgcag gtggggatga
     2161 gcagtgccgg accaggtgca gcagtcagtc gtgcctccag ggtgctctcc tcaggcttgc
     2221 ctatgccatt ggagcttgat acgcccactg cggttcctat gaccgcatag cccgaaccca
     2281 tgatctgatc caggtgagag cctgccggcc accaggaata aaggtcatct gtgtattgcc
     2341 attcagcccg accccgttgc agatgacggt tgtgagcgaa ggccatcacc cttcccctct
     2401 ggctgccctc ccgggacgtt atatacgcca ggttttccgc catcatggca tcacgaatgc
     2461 caaggagcct gcttgttcgg tcgagtgagt ttccggcaag ctcggcatga tagctaagct
     2521 gttgccgggc aagggctgca taatgcactg cctcctgata gcgagctgga tcgcttccgg
     2581 ccaccagctc ggggcggcga acgagcagct cctcatagag gtcctccact tccgcgcgca
     2641 gcgcgatcgc attgggagag cggccaatgg attttgccgg atcgatcaga gcatccggat
     2701 tatcccaatc ggattcctgg cccaggaagg gttcgatcct ctggcgatgc tcctctcctt
     2761 gaaccgggtc gattgtgccg aggaagtcca ggacgaagta tatcagctgc tttggactgt
     2821 cgctggccaa catctccgtg gggctgtcaa agccgtagaa atggagcttg tgggcatgag
     2881 acggatccga attgtactct ctcatccact ccaccagctc tcgattggca tcaaggcggc
     2941 caaagccgtg gctgaagccc aactccagga tatcatcgaa tgaatccggg ccctggccgg
     3001 cgatatactg gtccaccagc cgcccgcggg gaaagctgct ctcaatggcc atggccacga
     3061 agccatgatt ctccaccagg cgaatgaaaa gccggttgcg caagaggagg atgtcgctct
     3121 tcccatggag ggcttctcca aagccaagga gcctcactgt gtcccctatt ctggccatag
     3181 ccctatctat ggcatcgttc aattcggttg aatcgagaga gaatgggatc gcctgggcca
     3241 agatccaatc ttccagggtg gaatacccgg gctcaaagcc gcctttgc
//
LOCUS       DAOSGP010000151         3272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_27445_length_3272_cov_15.595586, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000151 DAOSGP010000000
VERSION     DAOSGP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27445_length_3272_cov_15.595586"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..779)
                     /locus_tag="PLV49_05080"
     CDS             complement(102..779)
                     /locus_tag="PLV49_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOG98253.1"
                     /translation="MSVVVQAKMNKIMNRIEDPREVLEVSYEKQLQLLQNVKRGVAEV
                     TTSKKRLELQKAKLQMNIDKLDGQAKEAISASREDLARKALESKALMQAQLNTLERET
                     KELNDQQLKLQAAETRLATKVEAFRTKKETIKAQYTAADAQVKITESVTGISEEMADV
                     GLAVQRAEEKTEDMKARSAALDELLESGTLTDYSGGDEIESELARVKAKNTVDDEMAR
                     LKAEMNK"
     gene            1002..1232
                     /locus_tag="PLV49_05085"
     CDS             1002..1232
                     /locus_tag="PLV49_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGF-CTERM sorting domain-containing protein"
                     /protein_id="HOG98254.1"
                     /translation="MRKSILALLLALFAIMALAAPIYAEDDDDDEDDEEENEASSESE
                     SDSDEKSTPGFGFAFAVAGALAGARLIRSKNH"
     gene            1326..>1738
                     /locus_tag="PLV49_05090"
     CDS             1326..>1738
                     /locus_tag="PLV49_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOG98255.1"
                     /translation="MSFAFFPAVDLRGGKCVQLVGGVPGTEVVALDNPLAQAERWIDE
                     GADHLHIIDLDGAIQGKRVNAPILAQIAKELDVYIQVGGGIRSEEEARDVLRLGVDRV
                     ILGTAALKDPDMIVQLAEEHGRERIMVALDVKRGDV"
     assembly_gap    1739..1838
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1929..2495
                     /locus_tag="PLV49_05095"
     CDS             1929..2495
                     /locus_tag="PLV49_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HOG98256.1"
                     /translation="MAKRNGRGLGLFRDCRAQATVNLDGSGQAKAASGSGFLDHMLLA
                     LARTANMDLIAESEEGLWRAQSLGKAIGLALDDALGDRSGICRYGSASVPMDESLADV
                     ALDFSGRPYLIISGEFRGERIGDFEVMLLEPFLEALSTAAGLTVHIRFYGENDHHKME
                     CIFKALGLALRQAAAKGGMGIPSTKGVI"
     gene            complement(2532..>3272)
                     /gene="surE"
                     /locus_tag="PLV49_05100"
     CDS             complement(2532..>3272)
                     /gene="surE"
                     /locus_tag="PLV49_05100"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOG98257.1"
                     /translation="GLEAAAKSVRGLGEVTVAAPSGQKSGVGRSISVFEPLRYAEVNL
                     NGFKAYAVTGTPVDSVIIGIFAILKEMPDLVISGINVGENISTDTVTTSGTIGAALEA
                     ASYGIPAIAASIQAVDQGDKFDMHHGAKHSFDVAALFLRRIATRVLEHGLPEGVDLLN
                     LNVPVGASEETEIKVTRLAKKIFKTAVQERFDPRGRPYYWIDGELICCDEEGTDVQTI
                     YQDKLVSLTPLSLDSTARIDLKEIEKLF"
BASE COUNT          681 a          858 c          864 g          769 t
ORIGIN      
        1 gccattcttc acatgatcca ctatctgatt gtcaatttta ttcagatcgt ccagatggct
       61 gtcagcaaag cgaaactgac cctctcccag gattctgatg atcatttatt catctccgcc
      121 ttcaatctgg ccatctcatc atctacggtg ttcttggcct tgacccgtgc caattcactc
      181 tcgatctcat cccctcccga gtagtcggtg agagttccgg attccaggag ctcatccaaa
      241 gcagcagagc gggctttcat atcctcagtc ttctcttctg ctctctgaac cgccaggcct
      301 acatccgcca tctcctcgct tatgccggtc accgactcgg tgatcttgac ctgagcatcg
      361 gcggcagtat actgggcttt gatggtctcc ttcttggtgc ggaaggcctc caccttggtg
      421 gccaggcggg tttcggctgc ctgcagcttc agctgctgat cattcagttc tttggtctcc
      481 ctttccaggg tattaagctg ggcctgcatc aaggccttgc tctccagggc ctttcgcgcc
      541 aggtcctccc ggctggcaga aatggcctcc tttgcctggc catcaagctt atcgatattc
      601 atctgcagct tggccttctg caattcaagc ctcttcttgg aggtggtcac ctctgccacc
      661 cctctcttga cattctgcaa tagctgcagc tgcttttcat aagagacctc aaggacctct
      721 ctgggatcct ctatcctatt catgatctta ttcatctttg cctgcacgac aacactcatc
      781 cgctctaaga agcccatatc aattctcctt cagcctatct gttctccacc gtctcttgat
      841 tggatgcaaa ttacatatac caatgtcgtt ttagatattt ataggctctt gtgcaatgga
      901 tgccatttgt gtgcttgttg ctgcaattga gcagaggatg cggctaaatt aagggaaagg
      961 atgaattacg atgaaatagg tttgaatccg gaggtgaata gatgagaaag tccatattgg
     1021 ctcttctttt ggccctgttc gccatcatgg cattggcagc accgatctat gccgaggatg
     1081 atgatgacga tgaggatgat gaggaagaga acgaggcttc cagcgagagc gaaagcgact
     1141 ctgatgagaa aagcactccc ggattcggat ttgcatttgc agtggcaggc gcgctggcag
     1201 gagctcgcct aatcagatct aaaaaccatt gagatcgttt gatatcgatt gctcgagatc
     1261 aattgatcgt ctcttttttc cagccatcga aacgattaag gtctattcga tgccaattgg
     1321 ccttcatgag ttttgcattc tttcctgcag tggacctgcg aggcggcaag tgcgtgcagc
     1381 tggtgggagg agtcccagga actgaggtcg tggccctgga caatcccctt gcccaggcag
     1441 agcgctggat cgacgaaggc gctgaccacc tgcacatcat cgacctggac ggggcgatcc
     1501 agggcaagag ggtgaacgcc cccatcctcg ctcagatcgc aaaggagctg gacgtctata
     1561 tccaggtagg ggggggcatt cggtcagaag aggaagcgag ggatgttctc cgcctgggcg
     1621 tcgatcgggt gatcctgggc actgccgccc tgaaagatcc cgatatgatc gtccaattgg
     1681 ctgaggagca cggccgggag agaattatgg tggccctgga tgttaagcga ggggatgtnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gcaggagctg tggccgggac
     1861 ggccatttac accggaaaat tgagcgtctc agctactctg caggccctgc gaagataggt
     1921 gatggtaaat ggcgaaaaga aatggaagag gattgggatt attcagagat tgcagggccc
     1981 aggccacagt taacctggac ggatcaggcc aggcaaaagc cgcctcagga tcgggatttc
     2041 ttgatcatat gctgctcgcc ctggcgcgca cggcgaatat ggacctgata gcggaatcag
     2101 aggagggctt gtggagagcc cagtctttag gaaaggccat cggccttgct ctggatgatg
     2161 ccctggggga caggagcgga atatgccgtt acggctcggc ctcggttccc atggacgagt
     2221 ctttagcgga cgtggctctg gacttcagcg gccgacccta tctgatcatc tccggagagt
     2281 ttcgggggga gaggatcggg gacttcgagg tcatgctcct tgaacccttc ctggaggcgc
     2341 tttctactgc agccgggctg acggttcata tccgcttcta cggggagaac gaccaccata
     2401 agatggaatg catcttcaag gccctgggac ttgccctgcg ccaggcggcg gcaaagggtg
     2461 gaatgggtat ccccagcacc aaaggagtaa tctgagggta gatcggaggt aaatctgagg
     2521 tcagatagag atcaaaatag cttctctatc tctttcagat cgattctggc cgtggaatcc
     2581 aggctgaggg gggtcaggga taccagcttg tcctgataga tggtctgcac gtcagtcccc
     2641 tcctcgtcgc agcatatcag ctccccatca atccagtagt aaggccggcc acgggggtca
     2701 aaacgctcct gcactgccgt cttgaagatc ttcttcgcca ggcgggtcac ttttatctcc
     2761 gtctcctcgc tggcaccaac tgggacattc aggtttaaga gatccactcc ttcaggaagg
     2821 ccgtgctcta agacccgggt cgctattcgg cgcagaaata gagctgccac atcaaagctg
     2881 tgcttggctc catgatgcat atcgaacttg tcgccctgat caacggcctg aattgaggca
     2941 gcaatggcgg gaataccgta gctagcggcc tccagagccg ctccgatggt accgctcgtg
     3001 gtaacggtat cggtgctgat gttctctccc acattgattc ccgagatgac cagatcaggc
     3061 atctccttaa ggatggcgaa gatgccaatg atgaccgaat ccaccggcgt tccagtgacc
     3121 gcataggctt taaaaccatt caggttcacc tcggcatagc gcaggggctc aaagacggat
     3181 atagatcttc ccacgccgct cttctgtccc gagggcgcgg ctaccgtcac ctcccccagg
     3241 cctcgaacgc tctttgccgc cgcctcgaga cc
//
LOCUS       DAOSGP010000152         3246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_27763_length_3246_cov_16.810404, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000152 DAOSGP010000000
VERSION     DAOSGP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27763_length_3246_cov_16.810404"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..816
                     /locus_tag="PLV49_05105"
     CDS             76..816
                     /locus_tag="PLV49_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG98258.1"
                     /translation="MEIIFLGTNGWYDSDTGNTICTLINSEKYHIILDAGNGISKADQ
                     YIDDDLAVYLFLSHFHLDHIEGLHILNKFHFSQGLKIFGQKGTREVLNTIVNDPFTVP
                     LMKLPYPVEVGELLPGPYKMPFALECEFLLHASPTLGYRFDLEGKIIAYCPDTGVCEG
                     AVHLAGDSDLLIAECSHKPGESSPAWPHLNPQDAADIARRAGAKRLALTHFDAGRYRS
                     IQERIDAVASVKDYQDIIVGKDGLKLHI"
     gene            complement(920..1051)
                     /locus_tag="PLV49_05110"
     CDS             complement(920..1051)
                     /locus_tag="PLV49_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98259.1"
                     /translation="MHDATCAECGKACQVPFKPDGSRPVYCSDCYQKHRPARPPRRY"
     gene            complement(1250..>3246)
                     /locus_tag="PLV49_05115"
     CDS             complement(1250..>3246)
                     /locus_tag="PLV49_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG98260.1"
                     /translation="RKAAWEAARVTVSTPQVIENDLLSRRIDLEDISLIIFDEAHRAV
                     GNYAYVYIAERYAREGTNPLVLGITASPGSEKEKIAEICTNLCIENIQTRSEKDSDVA
                     PFVHSREIEWVKLTVPKELLDIRTAIEEVLRDRIDDLNRLGISPARIDPRATKKELLG
                     LQAMLMSSAKRQANQATFKGISILAEVLKLYHAVELAETQGTEALLKYFQRLQGEAIS
                     RSGSKASRRIMEDPKFRQAMQALEELKVEHPKPAMVRKILTEQIEAKPESRIMVFTNY
                     RDTASALIRFLKEDPKIKAVRFVGQSSRADDEGLSQKKQAEILQRFRAGEYNVLIATS
                     VGEEGIDIPATDMVLFYEPVPSEIRSIQRKGRTGRARSGRVVVLMARGTRDEAYYWIS
                     DRKEKTMNKQMRTLSSQASQSPSSSSKDFLPRQLEITEDNPERQNRKGLERLVDWTEA
                     DEERDKIYVDPRERGMARLLEGRGAEVTLKNLEVGDYVVSDRVAIERKTAQDFVASII
                     DPERNLFRQIADLARSYERPVLILEGRDLYSRQVSASSIQGALASVAVDYGVPIIPTE
                     DQEDTASVITMLAARERREGHEPKLHGHKTARTLKEQQEYLISAIPSVGPSVARKLLR
                     HFGSIEKVIAASKEELQEVDMVGPKIAERIRELVGVEYKG"
BASE COUNT          675 a          951 c          807 g          813 t
ORIGIN      
        1 cgctccggga gagacgaaat ccatacccat tcaccgcatc aggcgcatct cttattgacc
       61 caatgaggca gaaagatgga gataatattt ctaggaacca acggctggta tgacagcgac
      121 accgggaaca ccatatgtac cctgatcaac tccgaaaagt atcacataat ccttgatgcg
      181 gggaacggca tcagcaaggc agaccagtac atcgatgacg atcttgctgt ctatctcttc
      241 ttgagccact ttcacctgga ccacatcgaa ggcctgcata tattaaacaa gtttcacttc
      301 tctcaagggc tgaagatatt cggccagaag ggcaccaggg aggttctgaa caccatagtc
      361 aatgatccct tcaccgttcc tctaatgaag cttccttatc ctgttgaggt tggcgagctt
      421 ttgcccgggc cctataagat gcctttcgcc ctggagtgcg agtttctgct tcatgcctct
      481 cccaccctcg gctatcgctt cgatctggag ggaaagataa tcgcctactg tccagatacc
      541 ggcgtctgcg agggtgcagt tcatctggca ggtgactcag acctattgat agctgaatgc
      601 tcccataagc ctggggagtc aagccctgcc tggcctcacc taaatcccca ggacgcagcc
      661 gatatcgccc ggcgggcagg agccaaaagg ttggccttaa ctcatttcga tgcaggcagg
      721 tacagaagca tccaggagcg tattgatgcc gtggcatcgg taaaggacta tcaggatata
      781 atcgtgggaa aagatggctt gaagctgcac atctgaagat attaaaaaat tcgaaaaaag
      841 ctttctaaaa atttgctttg cagggcttaa aaaattgacc ccgcttatac agcaatccca
      901 ttagggatga attcccagtt tagtatcttc tgggtggtct ggctggtcta tgcttctggt
      961 agcagtcgct gcaataaact ggcctggagc catcgggctt gaagggaacc tgacatgcct
     1021 ttccgcactc tgcgcaagtg gcatcgtgca tctctctcgg gccgctgctg tagccgcctc
     1081 tgcttccgct tccatatctt tccatttaaa ctcacttacc tttggcatcg attccgtgaa
     1141 tcgatcctac atactacaaa acatcccatc aaactcaatt gaagcgacgc ttatgcagca
     1201 tcaccagagt ggtttaattg ccttatatac ttgttggtta acacaaatct catcccttgt
     1261 actcaactcc caccagctcc cggatgcgct cagcgatctt aggcccgacc atgtccacct
     1321 cttgcagctc ttctttgctg gcagcgatca ctttttcaat tgacccaaag tggcggagga
     1381 gttttcttgc caccgacggc cccacgctgg gaatggcgga tatgaggtac tcctgctgct
     1441 cttttagcgt tctggcggtc ttgtggccat gcagcttggg ctcatgcccc tccctccttt
     1501 cacgagcggc gagcatggtg atgaccgatg ccgtatcctc ctggtcctcc gtgggaatga
     1561 tgggcacccc ataatcaaca gccaccgaag ccaatgctcc ctgaatggag cttgcgctca
     1621 cctggcggga atacaggtcc cttccctcca ggatcaagac cggccgctcg tagctccggg
     1681 caagatctgc tatttggcgg aagaggtttc tctccggatc gatgatcgag gccacgaaat
     1741 cctgggcggt cttgcgctcg atcgccactc gatcgcttac cacatagtcg cccacctcca
     1801 ggtttttcag ggtgacctct gcacctctcc cctccagaag cctggccatt ccacgctctc
     1861 tgggatcgac atagatcttg tcccgttcct catctgcctc tgtccaatcc accagcctct
     1921 ccagcccttt cctgttctgc ctttctggat tatcttcggt tatctccagc tggcggggaa
     1981 ggaaatcctt ggagctgctc gagggtgact gggaagcctg gctgctcaga gttcgcatct
     2041 gcttgttcat agtcttctct ttgcggtcgc taatccagta gtaggcttca tcccgagtgc
     2101 ccctggccat caggaccacc acccttccgc tcctcgccct ccccgtcctg cccttgcgct
     2161 gaatgctgcg aatctccgaa ggaaccggct cgtagaagag gaccatatca gtggccggaa
     2221 tatcgattcc ctcctccccc accgaggtgg cgatgaggac attgtactcc cctgccctga
     2281 acctttgcag gatctcagcc tgcttcttct ggctcaggcc ctcatcatct gcccggctgg
     2341 actggcccac aaagcgaacg gccttgatct tgggatcctc ttttaaaaat cggatgagag
     2401 cggaggcggt gtccctgtaa ttggtgaata ccatgatccg ggactcgggc ttggcctcaa
     2461 tctgctcggt cagtatcttc ctgaccatgg caggcttggg atgctccacc ttcagctcct
     2521 ctaaggcctg catggcctgg cggaacttgg gatcctccat aatccggcga gaggccttcg
     2581 agccgctgcg ggagatggcc tcgccctgca gccgctggaa gtacttgagc agagcttccg
     2641 tgccctgggt ctcagccagc tccacggcat gatagagctt gagaacttct gccaggatgg
     2701 atatgccctt gaatgtggcc tgattggcct gtcttttagc tgaggacatg agcatggcct
     2761 gaaggccgag gagctccttt tttgtggctc tgggatcgat cctcgccggg ctgatcccca
     2821 ggcggtttag gtcatctatt cgatctctca gcacctcctc tatcgccgtt ctgatatcaa
     2881 gtagctcctt gggaacggta agcttcaccc attcgatctc cctggagtga acaaaaggcg
     2941 ctacatcaga gtccttctcc gaacgggttt ggatattctc tatgcagagg ttggtgcaga
     3001 tctcagcgat cttttccttc tcgctgcctg ggctggcggt gatccccagg accaggggat
     3061 tggtcccctc ccgagcatag cgttccgcaa tatagacata agcatagttt cccactgccc
     3121 ggtgggcctc atcgaagatt atcagggaga tatcctccag atcgatcctg cgagagagga
     3181 gatcgttctc tatcacctgg ggagtggata ctgttactcg cgcagcttcc caggcggcct
     3241 tcctct
//
LOCUS       DAOSGP010000153         3237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_27887_length_3237_cov_16.110308, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000153 DAOSGP010000000
VERSION     DAOSGP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27887_length_3237_cov_16.110308"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1183
                     /locus_tag="PLV49_05120"
     CDS             71..1183
                     /locus_tag="PLV49_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98261.1"
                     /translation="MIGKGLNGRLEKDASPHAALPLFWIALAAYLILSLSSIPPALAA
                     ESGAPIPPERFSADYYKVYGTPNLTASLDMSRIYQGEATVLTMTLTNRGRITSFEINE
                     EPAANKREEILAAQRELELEKQRTVAQDVSVLLRAENESAIDIKRSVAFPGNIREGQT
                     SPRLEFPLEAFENALPGSYRLLAQVNYTYQKDVAVKEDDDRPENPDVYYWYDSLSQTI
                     PIDLIVERRSGAQFEVLNVTPTSLRPGSEGNVVRIRVKNVGDDRATDLVARLRPESGL
                     YVSVDESPIPSLEPQEEAELLYKLDVSKDALPGKNYQLRILFQFSDSRRDDLTDWENA
                     YLRIEEGGREWALAILLFLALLAAVLILAVRRRKKA"
     gene            1189..2097
                     /locus_tag="PLV49_05125"
     CDS             1189..2097
                     /locus_tag="PLV49_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG98262.1"
                     /translation="MAVGAGPAVSARGVTKSFGQLRVLDGLDVDIPRGVTYCLLGPNG
                     SGKTTFIRAIVRLLRLDAGELMVLDQPVSRVEEIYSRIGYMTQHKALYPDLTVEENME
                     FYSGLYGIKGQDREVRIEELLRMVDLSEHRKRLAGALSGGMYQRLSLACTLVHRPELL
                     LLDEPTVGIDPRLRKTFWEYFQSMAEAGKTVIITTHLMDEAERCQMVGYMRAGRMAAQ
                     GSPEEILNQAGLGPRLRLWLAQPEDDAALLRSLGFEAEIDGGVVMVSLKSHAQIKDVL
                     AQISPRDIRLSEPRLEEAFLRLSEGA"
     gene            2094..3152
                     /locus_tag="PLV49_05130"
     CDS             2094..3152
                     /locus_tag="PLV49_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98263.1"
                     /translation="MKRPKPERVGVVALRVVRQLKRDRRTIGLILFAPVVLMILFGYA
                     LSGEMTGVQLGLVDGGGHEPLRDYIASLDDFDILYLGSQSDAEKLISEGRLDGAVVIQ
                     PEEVRVLLDSSSLQIADRVLAAVQTGVSREVSIRTDSYPPIITRYIFGYDLEMIDTIG
                     PAILGLVAFFFAFIIAAISFLRERTLGTLEKFMVSPLSRVEIVSGYILGFSLVAIIQS
                     ATTLLIVIYLFGVPMKGSPLDAFAIILLLGAGALALGSFVSNFARSEFQVVQFIPIVI
                     IPQIVLCGVFWPVQSVPGFLRPISNILPLTYASDALRAIMLKGASLSQILPDLIFLGA
                     FFLLMFTAATLMLKREVG"
BASE COUNT          645 a          915 c          971 g          706 t
ORIGIN      
        1 aaaagcatca tactcttatt cggatttttg tcatggcccg atcaaggcct gcaggaggag
       61 gaatgatccc atgattggca aaggactaaa tggcagatta gagaaggacg cctcccctca
      121 tgccgccctg cccctcttct ggatcgcctt agctgcatat ctgattctat ctctatcctc
      181 gatcccgcct gctctggccg ccgagagcgg tgcccccatc ccgccggaga ggttctcagc
      241 cgactactac aaggtctacg gcaccccgaa tctgaccgca tccctggaca tgtctcgcat
      301 ctatcagggg gaggcgaccg tcctcaccat gaccctgacc aaccggggcc gcataacctc
      361 ttttgagata aacgaggagc cagcagccaa caaaagggag gagatcctgg cagctcagag
      421 ggagctggag ctggaaaagc agaggactgt ggcccaggat gtctccgttc ttctccgggc
      481 ggagaacgag agcgcaatag atatcaaacg ttcggtggcc tttccaggca acatccgcga
      541 gggccagacc tccccccggc tggagttccc cctggaggct tttgagaacg ccctccccgg
      601 cagctaccgc ttgctggccc aggtcaacta cacctatcag aaggacgtgg cggtgaaaga
      661 ggatgatgac cggccggaga acccggacgt atactactgg tacgattctc tcagccagac
      721 aattcccatc gacctgattg tcgagaggag aagcggtgcc caatttgagg tcctaaatgt
      781 cacacccacc tcccttcggc cgggctcaga agggaatgtg gtccggataa gggtgaagaa
      841 tgtgggggac gaccgggcaa ctgacctcgt cgcccgtctc cgtccggaaa gcggcctgta
      901 tgtgagcgtt gacgagtctc ccatcccttc cctggaaccc caggaggagg cagagcttct
      961 ctataagctg gacgtctcca aggacgccct tcccggcaag aactaccagc taaggattct
     1021 cttccagttt tccgacagcc gccgggacga cctgaccgac tgggagaacg cctatctgag
     1081 aattgaggag ggaggacggg aatgggcatt ggccattcta ctttttctcg ccctgctggc
     1141 tgcggtgctg attttagccg ttcgcagaag gaagaaggcc tgaggtccat ggcagtcggt
     1201 gcaggtccgg cagtatcggc tcgtggcgtg accaagagct tcgggcagct gcgggtattg
     1261 gacgggctgg atgtggacat tcccaggggg gtgacctact gcctcttggg gcccaacggc
     1321 tcgggcaaga ccacattcat ccgggccata gtccggctgc ttcgcctgga tgctggagag
     1381 ctgatggttc tggaccagcc cgtctctcgt gtggaggaga tctactcccg gatcggttat
     1441 atgacccagc ataaggccct ttatcccgac ctgacggtgg aggagaacat ggagttttat
     1501 tctgggctct atggcataaa ggggcaggat cgagaggtca ggattgaaga gctgctgaga
     1561 atggtcgacc tctccgaaca ccgcaaacgc ctggcaggag ccctctccgg cgggatgtac
     1621 cagaggctct cactggcctg taccctcgtc caccggcctg agctgctctt gcttgacgag
     1681 cccactgtag ggatagaccc ccgcctgagg aagaccttct gggagtattt ccagagcatg
     1741 gccgaggcgg gaaagacggt gatcatcacc acccacctca tggatgaggc ggaacggtgc
     1801 cagatggtgg gctacatgcg ggcgggaagg atggccgccc agggcagccc ggaggagatt
     1861 ttgaaccagg caggcctggg gcccaggctc cgtctctggc tggcccagcc ggaggatgat
     1921 gctgctctgc tgcggtcgct gggatttgag gcagagatcg atggtggggt ggtgatggtc
     1981 tccctcaaaa gccatgccca gatcaaagat gtcttggccc agatatctcc ccgggatatc
     2041 aggctatctg agcccaggct ggaggaggct ttcttgcgcc tctcggaggg ggcatgaaaa
     2101 ggccaaaacc ggagcgtgtg ggcgttgttg ccctgagggt cgtccgtcag cttaaaagag
     2161 accggcgcac catcggcctg atcctgttcg ctcctgtcgt cctgatgata ctcttcggct
     2221 atgccctctc cggagagatg acaggggttc agctgggtct ggtggacggc ggaggccatg
     2281 aaccgttgcg tgactacatc gcatccttag acgacttcga tatcctctat cttggctcgc
     2341 agtcggacgc agagaagctc atctctgagg gcaggctgga tggtgcggtg gtcatccagc
     2401 cagaagaggt gagggttttg cttgactcca gcagcctgca gatcgcagac agggttttgg
     2461 ctgctgtgca gaccggagtg agcagggagg tgtcgatccg gaccgattca tatccgccca
     2521 tcatcacccg ctacatcttc ggctacgacc tggagatgat cgataccatc ggcccggcga
     2581 ttttaggcct ggtggcattc ttcttcgcat tcatcatcgc cgccatatcc tttctccggg
     2641 agaggacctt ggggacgctg gagaagttca tggtctcgcc cctgagccgg gtggagatcg
     2701 tctccggcta catcctgggc ttcagcctgg tggccatcat ccagtcggca accactcttt
     2761 tgatcgtgat ctatttattc ggcgtgccaa tgaagggaag ccctctggat gctttcgcca
     2821 taatcctctt gctgggcgca ggcgcacttg ctctgggctc gttcgtatcc aacttcgcta
     2881 gaagcgagtt tcaggtggtg cagttcatcc ccattgtaat cattcctcag atagtgctgt
     2941 gcggggtgtt ctggccggtt cagtccgttc cgggtttcct ccgccccatc tccaacattc
     3001 tgcccctgac ctatgccagc gatgccctga gggcgataat gctcaaggga gcatcgctat
     3061 ctcagatatt gccggatctc atctttttgg gagccttctt cctgttgatg ttcaccgctg
     3121 ccacgctgat gctcaaaaga gaggtgggct gaaggctggc ggcattagca tgatatggag
     3181 aattgagagc cagctcgatt gcctgattgc ctaggccagc ttgcttttca ccttggc
//
LOCUS       DAOSGP010000154         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_28251_length_3209_cov_4.588142, whole genome shotgun sequence.
ACCESSION   DAOSGP010000154 DAOSGP010000000
VERSION     DAOSGP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28251_length_3209_cov_4.588142"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLV49_05135"
     CDS             <1..303
                     /locus_tag="PLV49_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HOG98264.1"
                     /translation="FSYAPTSEPLVNIRFNLDLAKEKGIISEQFREKLFLAARRLYFP
                     QRSYQSMLQEGSKDLPPEEMKRLRDFLEKERRDFKMEDALLALVRAGELAESSSPG"
     gene            424..1326
                     /gene="nadA"
                     /locus_tag="PLV49_05140"
     CDS             424..1326
                     /gene="nadA"
                     /locus_tag="PLV49_05140"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOG98265.1"
                     /translation="MAIIDEILSLKEQRRAAILAHNYQIPEVQDIADLTGDSLELARA
                     AARLDCEVLVFCGVDFMAETAAILSPEKTVVLPAKGAWCPMAHMITPEELMDLKALHP
                     QAAVVCYVNSTAEIKAESDVCCTSANGVEVVSSISEKEVIFVPDRNLAAYVARHTDKK
                     VIPWNGYCYVHDQFTAAEVEAARARHPQAEVLVHPECRPEVIDQADFVYSTSGMGRHA
                     RSSPAREFIIGTETGMIHKLKKDCPDKEFYPLSRNAICQNMKKTNLSLVLQALQTLEP
                     QVSVPEETAVRAKKAIERMLAVKV"
     gene            1523..1594
                     /locus_tag="PLV49_05145"
     tRNA            1523..1594
                     /locus_tag="PLV49_05145"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1555..1557,aa:Cys,seq:gca)
     gene            1906..>3209
                     /locus_tag="PLV49_05150"
     CDS             1906..>3209
                     /locus_tag="PLV49_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG98266.1"
                     /translation="MYIGSTDSLGLHHLVYEVVDNSVDEALGGYAKGINVLLNRDGSV
                     SVADDGRGIPVDNHPQFGRPALEIVMTVLHAGGKFDHESYSVSGGLHGVGVSVVNALS
                     EWMEVEVRRNGRIYWQRYQQGSVASDVEVRGHSEDTGTTVTFKPDGNIFEETEFNFDT
                     LSSRLRELAFLNRGLSITIKDERTEKENDFCYEGGIISFVEYLNANKETLHKPPLYFT
                     RSKNGTAVEVALQYNNSYNENVFSYANNINTREGGTHLMGFRAALTKTVNEYARANKI
                     FKNETKLGGEDLREGLVAIVSVKLPDPQFEGQTKTRLGNSSIRGLIESMVSEGLSEYF
                     EENPLVATTIVEKAGEALRAREAARKAKELTRRKNALSSGGLPGKLADCSNNEPGQCE
                     IYIVEGESAGGSAKQGRNRHFQAILPLRGKILNVERARLDKIL"
BASE COUNT          773 a          841 c          920 g          675 t
ORIGIN      
        1 ttcagctatg ctcccacatc cgagcctctg gtcaacatcc gcttcaatct ggatctggcg
       61 aaagaaaaag gcataatctc tgagcaattc cgggagaagc tctttcttgc cgcgcgccgc
      121 ctctactttc cccaaagaag ctaccagtcc atgctgcaag agggatcaaa agatctcccc
      181 ccggaggaga tgaagcggct gagagacttt ctggaaaagg agagacgcga ctttaagatg
      241 gaggatgccc ttttggccct ggtgcgggct ggagagctgg ctgagtcatc ctctcctgga
      301 tgagatcggc tgtaagaccg caggagatgc aaactaaaat ctactttaat tgaagtttta
      361 gtatcaatct aatctactta aatagcttga aataatagtt tctcaaaaag ggggccagtt
      421 cccatggcga tcatagatga gattctctct ttgaaagagc agcgacgtgc tgcaattctg
      481 gcccataact accagatccc ggaggttcag gatattgccg acctgaccgg cgactccctg
      541 gagctggcca gagcagcagc ccgtctggac tgcgaagttc tggtcttttg cggagttgac
      601 ttcatggctg agactgcggc cattctctct ccggaaaaga cggtggtgct gccggcgaag
      661 ggcgcctggt gccccatggc ccatatgatc accccggagg agctcatgga cctcaaagcc
      721 ctccaccccc aggcagcggt ggtatgctat gtcaactcca cggcggagat caaggcagag
      781 agcgatgtct gctgcacctc ggccaatgga gttgaggtcg tctcctccat ctcggaaaag
      841 gaggtgatct ttgtgcccga ccgcaatctt gccgcctatg tggcccgcca cacggacaag
      901 aaggtcatac cctggaacgg ctactgctat gtgcacgatc aattcactgc tgccgaggtg
      961 gaggcggccc gggccaggca tccccaagca gaggtgctgg ttcatcccga atgccgcccg
     1021 gaggtcatcg atcaggccga tttcgtttac agcacatcgg ggatggggag acatgctcgg
     1081 tccagtccag cccgggaatt tatcataggc acggagacgg gcatgatcca taagctgaaa
     1141 aaggactgtc cggacaagga gttctatccc ctgagcagga atgccatctg tcagaatatg
     1201 aagaagacca atctctcttt agtcttgcag gcgctgcaga ccttagagcc gcaagtcagc
     1261 gtgcctgagg agacagccgt ccgggccaaa aaagcgattg agagaatgct ggcggtcaag
     1321 gtttgagcac atcgctcgcc tggcctggat caagcagatt aggatcgggc agattatctt
     1381 tttcccagcc tggcctctat gggtaaggat ggcaatcttt atagaggatg aaatcttcct
     1441 gcccctcgtc gggcgaaaga gcgcgctcca accgataaag gtttaaatca aactgacact
     1501 ctgggacgcg gtagcgccta ttgccaaggt ggcggagcgg ctacgcaaat gcctgcagag
     1561 cattaccact ccggttcgaa tccggacctt ggctttcttt aatttgaggc ggcttggttc
     1621 ataattttta aagatgtctt cctggttgga gaggagaaga ttaatcccta tgtccacatt
     1681 tcatttgccg ggagatccat gcgccatcag gtcagcagca ttgtatggaa acattagata
     1741 ataattatga tatacttttg gggaaaggct tatatcgtct caggttattt cattacagtc
     1801 ctaattattc agggggatta atcagttttg agccaagtaa cgggatatga cgcaagccag
     1861 attcaggttc tcgaaggcct tgaggcagta aggcgcaggc cgtccatgta tataggcagc
     1921 accgacagct tggggctgca ccacctggtc tatgaggtgg tggacaacag tgtggatgag
     1981 gccctgggtg gctacgccaa ggggatcaat gtgctcctaa accgggacgg cagcgtctcc
     2041 gtagcagatg atggccgggg catccccgta gacaatcatc cccaattcgg caggcccgct
     2101 ctggagatcg tcatgactgt tcttcatgcc ggaggcaagt tcgaccacga gtcctatagc
     2161 gtctcgggtg gactgcatgg cgtgggcgtc tccgtggtca atgccctttc cgagtggatg
     2221 gaggtagagg tccgccgcaa cggtcgaatt tattggcagc gctaccagca gggcagcgtg
     2281 gccagcgatg tagaggtgag gggccactct gaggatacgg gcaccactgt caccttcaag
     2341 ccggatggca acatattcga ggagaccgaa ttcaacttcg atactctatc gtcccggctg
     2401 agagagctgg cctttctcaa ccggggcctc tccataacca tcaaagatga gagaactgaa
     2461 aaggagaacg acttctgcta tgagggcggc atcatctcct ttgtggagta cctcaatgcc
     2521 aacaaagaga cgctgcataa gccgccttta tacttcaccc gctccaagaa cgggacggca
     2581 gtggaggttg ccctgcagta caacaactcc tacaacgaga atgtcttttc ctatgccaac
     2641 aatatcaaca cccgtgaggg cggcactcat ctcatgggat ttcgtgccgc tctgaccaag
     2701 acggtcaacg aatacgcccg ggccaataag atattcaaga atgagaccaa gctgggcggc
     2761 gaagacctgc gggaggggct ggtggcaatc gtcagcgtga agcttcccga tcctcagttc
     2821 gagggtcaga ccaagacccg gctgggcaac agctccatcc gggggctgat agagtccatg
     2881 gtctcggagg ggctatcgga gtacttcgag gagaaccccc ttgtggccac caccatcgtg
     2941 gagaaggcgg gagaggctct gcgggcgagg gaggcggcca gaaaagccaa agagctcacc
     3001 cgccgcaaga atgccctcag ctcgggtggc ctgccgggaa agctggccga ctgctctaat
     3061 aatgaacccg gccaatgcga gatatacata gtggaaggcg agtccgccgg gggaagcgcc
     3121 aagcagggca gaaaccgcca ttttcaggcc atactgcccc tgcggggcaa gatcctcaat
     3181 gtggagaggg ccaggctgga caagatcct
//
LOCUS       DAOSGP010000155         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_28508_length_3189_cov_21.291002, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000155 DAOSGP010000000
VERSION     DAOSGP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28508_length_3189_cov_21.291002"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLV49_05155"
     CDS             <1..267
                     /locus_tag="PLV49_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98267.1"
                     /translation="IMRAIREYLKHKPCLRGQILDRGELKRVAQACGLSPQEARMELR
                     RLGFILTRNNHGLPVWTIPKADRSEGMASNEMLNRASKRGSLTG"
     gene            382..990
                     /locus_tag="PLV49_05160"
     CDS             382..990
                     /locus_tag="PLV49_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG98268.1"
                     /translation="MKGIDLEIENGEFVALMGPSGSGKSTLLNIIGCLDRPTSGRFLL
                     LGKDISRTPNEELARIRREEMGFIFQTFNLIARISVLQNVEIPMMLRGVPRGVRRERA
                     MKLLESINVAHRADFGPQNISGGERQRVAIARALANDPQIIIADEPTGNLDLKNSDEV
                     MKILNQLHEEGRTIIMVTHNPEITENCSRVIRLRDGRILENC"
     gene            991..1908
                     /locus_tag="PLV49_05165"
     CDS             991..1908
                     /locus_tag="PLV49_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOG98269.1"
                     /translation="MKRILIIILALLITGLAGGYEYRMPVNIQSSTTPAVLMPGDEAI
                     LAIQMQNGAAAYGVGKDAGVSSLSSNVLLSTPINRTLLQGTSELEVIDPYRYDLGMVG
                     PNDQITVFYKVRARENVSAGTTLLNFTVLGGYDMITINRNIPIKVDPSAVSMAIIDST
                     SSSPSTTPSKATVNLNVANPRENTLNAVTIVPSAQGMRFSPERYYIGTMDPDEVFTIS
                     FGVEYTDLGKTARNPSNVSFVAEFKNGDTWHESEPYIATYTPPLQADRPNNNLLLMAI
                     GALVLLAAGGYLYRKKRLSKNGDGGKGSS"
     gene            1948..3177
                     /locus_tag="PLV49_05170"
     CDS             1948..3177
                     /locus_tag="PLV49_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98270.1"
                     /translation="MRLQDMLEFISLGFRSDRFKTLMSSLGIIIGVLAIVVMLSVGEG
                     LYSGVSSQFSTLDLDVIHVIPGSARFGGPDGEPGSRNAAEPAKFTDKDTKVLENVVGV
                     KNVAPQTGASVVISFRNTNSSGSLTGVDPDKEQDLKEKVAQGRWLSGSDYRSIVIGNG
                     ISQEMFRMKVTPGNKIRLYYRDRPMDFTVVGVLEEEEESGFGGGMGDSADNAMYVTHK
                     AMEELLDEENYYYQTFQVTVSDPDQADYIIERIINDLRRYHKDEAYDATTARDMLSTL
                     MSTLSMIKYALAGIGAISLLVGGIGIANVMMLTVKERIREIGTMLALGATVEDIRRQY
                     LLEAGVLGMVSSLIGIVLGVGTSLLIGSLAGLPSSITPESIILGVLFGVLTTTIAGYY
                     PANKAAKLDPIEALRAE"
BASE COUNT          777 a          829 c          936 g          647 t
ORIGIN      
        1 ataatgagag cgatcagaga atacttaaag cataaaccat gtctgagggg ccagatcctc
       61 gatcggggcg aactgaagcg cgtggcccag gcctgcggat tgagccctca ggaggccaga
      121 atggagctga gacgcttggg tttcatcctg acccggaaca atcacggcct gcccgtctgg
      181 acgattccta aagccgatcg gtcggagggc atggccagca atgaaatgct caaccgggct
      241 tctaaaaggg gcagcctgac aggctaacgg aaaatatatg aagataaaaa agataagaga
      301 gagctgagtg ccattcctgt acttatagag ctgaaggaca tccgtaagac ctatctcatc
      361 ggcaggagcg aatatcctgt tttgaagggc atagatctgg agatagagaa tggggagttc
      421 gtggcgttga tggggccatc tggcagcggc aagtccaccc tgctgaacat catcggctgc
      481 ctggaccggc ccactagcgg gcgcttttta ctgctgggca aggatatcag ccggacgccg
      541 aacgaggagc tggccaggat ccggcgggag gagatgggtt tcatcttcca gaccttcaac
      601 ctcatcgccc ggatatctgt cctgcagaat gtggaaattc ccatgatgct gcgcggagtc
      661 ccccgcgggg tgagacgaga gagggccatg aagctcttgg agagcataaa tgtcgcccac
      721 cgggcggact tcggccccca gaacatctct ggcggggagc ggcaaagggt ggcgatagcc
      781 agagctcttg ccaacgatcc ccagatcata atagccgatg agcccacggg caacctggac
      841 ctcaagaata gcgatgaggt catgaaaatc ctcaaccagt tgcatgagga gggcagaacg
      901 atcatcatgg tcacccacaa tccggagata actgagaact gcagccgggt catccggctc
      961 agagacggac gaattttgga gaattgctag atgaagagaa tactaatcat cattttggcg
     1021 cttctgataa cgggcctggc aggaggatat gagtatcgca tgccagtgaa tatccagagc
     1081 agtaccactc ccgcagtgct catgcccggg gatgaggcga ttttagccat tcagatgcag
     1141 aacggagcag cagcctacgg cgtaggaaag gatgcagggg tctcaagcct ctcctcaaat
     1201 gtcctattat ccactcccat caatcgtaca ctgctccagg gcacctctga gctggaggtc
     1261 atcgatccct atcgctatga cctgggaatg gtcggtccga acgatcaaat caccgtattc
     1321 tataaggtca gggcacggga gaacgtgagc gcaggcacta cccttcttaa ttttacggtt
     1381 ctggggggct acgatatgat caccatcaac cgcaatattc cgatcaaggt cgatccttct
     1441 gcggtgagca tggccatcat cgactcaacc tcttcctctc catccacgac cccttccaag
     1501 gccaccgtca acctgaatgt ggccaacccc agggagaaca ccctcaacgc cgtgaccata
     1561 gtgccctcag cccaggggat gaggttctcc ccggagaggt actatatcgg cacgatggac
     1621 ccggatgagg tcttcaccat cagttttggt gtggagtaca ccgatctggg gaagactgcc
     1681 cgaaatccct ccaatgtcag ctttgtggct gagttcaaga acggcgatac ctggcatgag
     1741 tcagagcctt acatcgccac ttacaccccg cctctgcaag ctgaccggcc gaacaacaat
     1801 ctcctgctca tggccattgg tgccctggtc cttttggctg ctgggggcta cctctaccga
     1861 aaaaagaggc tctcgaagaa tggcgatggg ggaaaggggt catcttgaag gatctgaaat
     1921 gagcctgagc cttgttgggt ggcgagaatg agattgcagg atatgctgga gttcatctcc
     1981 ctgggattca gaagcgatcg cttcaagacg ctcatgtcct ccctgggaat aatcatcggc
     2041 gtcttggcga tagtggtgat gctctccgtg ggggaggggc tatactctgg tgtctccagc
     2101 cagttctcca ccttggacct ggatgtgatc catgtaattc caggcagcgc tcgctttggc
     2161 gggccggacg gggagccagg aagccgaaat gctgctgagc ccgccaagtt cactgataag
     2221 gacaccaagg ttttagagaa cgtggtgggg gtcaagaacg tcgcaccaca gaccggggcc
     2281 agcgtggtga tctccttcag gaacaccaat agctcaggca gcctcaccgg ggtggacccg
     2341 gacaaagagc aggatctgaa ggaaaaggtg gcacagggca ggtggctctc gggctcggat
     2401 tacaggtcga tagtgatagg caatggcata tcccaggaga tgtttcgcat gaaggtcact
     2461 ccggggaaca agatccgact gtactatcgt gaccggccca tggacttcac tgtggtaggg
     2521 gtgctagagg aagaggagga gtcgggcttt ggcggcggta tgggagatag cgctgacaat
     2581 gccatgtatg tcactcataa agccatggaa gagctcttag atgaggagaa ctactactat
     2641 cagacctttc aggtcacagt ctccgatccc gatcaggcgg attatataat cgagaggatc
     2701 ataaacgacc ttcgtcgcta ccataaggac gaggcctatg atgccaccac cgcccgggat
     2761 atgctcagca ccctgatgag caccctctcc atgatcaagt acgccctggc gggaatcggg
     2821 gccatatcac tgctggtggg gggaattggc atcgccaatg tgatgatgct caccgtaaag
     2881 gagaggatca gggagattgg gaccatgctc gccctggggg cgacggtaga ggacattcgc
     2941 aggcagtatc tgctggaggc aggggtctta ggaatggtct caagcctgat aggcatagta
     3001 ctaggggtgg gaacctctct tctcataggc tctctggccg ggctgccatc gtccataacc
     3061 cccgagtcga taatactggg tgttctgttc ggcgtcctaa ccaccaccat tgccggctat
     3121 tatccggcga ataaagcggc aaagctcgat ccgatcgagg cgctcagagc agaatgagcg
     3181 cctccgagg
//
LOCUS       DAOSGP010000156         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_28632_length_3178_cov_17.260327, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000156 DAOSGP010000000
VERSION     DAOSGP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28632_length_3178_cov_17.260327"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLV49_05175"
     CDS             <1..873
                     /locus_tag="PLV49_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HOG98271.1"
                     /translation="ELSEKEAEKLRLVADYDVVNFRFVPEEKILTPEDILGFRPSFEI
                     VGDIALIEDLEEKEAERVASALMATSKSIKTVIAPISDVEGEYRVRRFRHVAGEARTT
                     TTHKEHGLRYLIDLEGAYFTPRLGTERLRIAGLVGPDDTVFDMFAGVGPFSILMAKRC
                     RWVVAVDKNPVAVQYMRENARLNKVENIDILEGDANEIALRYQNAADHVIMNLPHSAS
                     LFLPAAIGAAKPGGTVHYYCIAPEDDLYRDELLIREAAEKMSAGIEVLYESIVRSYAP
                     HRLNVVIDFRVKKL"
     gene            complement(939..1958)
                     /gene="dph2"
                     /locus_tag="PLV49_05180"
     CDS             complement(939..1958)
                     /gene="dph2"
                     /locus_tag="PLV49_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HOG98272.1"
                     /translation="MDRALLTTGSEFVDFGYELDLDLAVHLIKKSGAARVGLQAPEGL
                     KRATPIVAARIAEETEAEVIISGDPCFGACDVDTRLLGEVDLMLHLGHAGIGEEPEKV
                     IFLEARMNEDLRGVVEKAIGELKSKRVGVATTVQHVHKLDGALEVLAEHGIEGLLGPA
                     SGRIKYPGQVLGCCYASARALDVDEYLFIGTGQFHPLGIALATGKRVLAADPVTCEIL
                     EIDPDPMLRRRFAAITRAADAKSFAVLVSKKSGQKRMELARRMMALGEARGREMFLVY
                     LDNIEPDRLLNLGARAAVSTACPRVALDDAAKYRIPILTPPEFEVLVGKREWEDYLFD
                     EIDDI"
     gene            complement(1927..2937)
                     /gene="fen"
                     /locus_tag="PLV49_05185"
     CDS             complement(1927..2937)
                     /gene="fen"
                     /locus_tag="PLV49_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HOG98273.1"
                     /translation="MGVDLGELLKRKQIEIKDLSGRWVAIDAFNTLYQFLSIIRQRDG
                     TPLMDRQGRVTSHLSGLLYRTTNLIEAGVKVAFVFDGEAPTFKAVTLAERSEIRDKAA
                     SAWEEARAAGEDGFKYAQAASRINSEIIEDGRRLILAMGLPVIQAPSEGEAQAAYMAA
                     KGDVDYAGSQDYDSLLFGAPLVVRNLAITGKRKLPRKNIYVDVEPEVINLEEGLANLG
                     ITHKQLVEIGIMCGTDFNSGLERVGPKTALKLIREKGDLESILADRDDKIEDFEKIRE
                     FFLHPPVTDDYKIALKKPVSEEIVSFLVDERDFDPERVEKTAKRLEEVYRSGQSTLDH
                     WF"
BASE COUNT          700 a          928 c          834 g          716 t
ORIGIN      
        1 gagctgtccg aaaaggaagc agaaaagctg cggctggtag cagattatga tgtcgtgaat
       61 tttagattcg tgcccgagga gaagatcctt accccagagg acattctggg ctttaggccc
      121 agctttgaga tcgtgggcga tatcgctctg atagaggacc tggaggaaaa agaggcggag
      181 agggttgcct cagccctcat ggccaccagc aagagcatca agacggtgat agcccccatc
      241 tctgatgttg aaggggagta tcgagttcga cggttcaggc atgtagcagg ggaggcgagg
      301 accaccacca cccataagga gcacgggctg cgctatctaa tagacctgga gggagcctac
      361 ttcacccccc gactgggaac tgaaaggctg aggatagcgg gcctggtcgg gcccgatgac
      421 accgtctttg acatgttcgc cggagtggga ccgttctcca tcctcatggc caagagatgc
      481 agatgggtgg tggccgtgga caagaatcca gtagctgttc agtatatgcg agagaacgcc
      541 cggctgaata aggtggagaa catcgatatc ctggagggag atgcaaatga gatcgcactg
      601 cgataccaga atgcagcgga tcatgttatc atgaacctcc cccatagtgc atccctcttc
      661 ctgcctgctg ccatcggggc ggcaaagccg ggagggactg tccactacta ctgcattgct
      721 cctgaggacg acctctaccg ggacgaattg ctgataaggg aggcagcgga gaagatgagc
      781 gcggggatag aggtgctcta cgagagcatt gtgagaagct atgcccctca caggctcaat
      841 gtggtgatag acttcagggt caagaagctt tagtgaagct ctgtttagtt ccctgctatt
      901 gatccttaaa gtgcttgctg aaagcttctc ctggtcgttt aaatatcatc gatctcatca
      961 aacaggtagt cctcccactc cctcttcccc accagcacct cgaactccgg cggggtgagg
     1021 atgggaatac gatacttggc ggcatcatcc aatgccactc tggggcaggc ggtagagact
     1081 gccgcgcggg cccccaaatt gagcagccgg tccggctcga tattgtccag atagaccagg
     1141 aacatctccc gacctcgggc ctcgcccagg gccatcatcc ttcttgccag ctccatcctc
     1201 ttctggccgg actttttgga gacgagaacg gcaaagctct tggcatcggc agctctggtg
     1261 atggctgcaa agcgcctgcg cagcatggga tccggatcga tctctaagat ctcgcaggtc
     1321 accggatctg ctgccagcac cctctttcct gtggcaagag cgatccccag gggatgaaac
     1381 tgtcccgtgc cgatgaagag atactcgtcc acatcaagcg ccctggcaga ggcatagcag
     1441 catcccagga cctgtccagg atacttgatc cttcctgaag ccggacccaa aaggccctcg
     1501 attccgtgct cggccaggac ctccagcgct ccatcgagct tatggacatg ctgaacagta
     1561 gtggcgaccc ctaccctctt tgatttgagc tctccgatcg ccttttccac cacgcctctt
     1621 aggtcctcat tcatcctggc ttccaggaag atgaccttct ccggctcctc tccaatgcca
     1681 gcatggccca gatggagcat cagatccacc tctcccaaaa gcctcgtgtc cacatcgcaa
     1741 gccccaaagc aggggtcccc ggagatgatc acctctgcct cggtctcctc tgcgatccgg
     1801 gccgctacta ttggcgtagc cctcttcagc ccctcgggag cctgcaggcc tactcgcgcc
     1861 gcaccgctct tcttgatcaa atgaactgcc agatcgaggt cgagctcata gccgaaatcg
     1921 acaaattcag aaccagtggt caagagtgct ctgtccactc ctgtagacct cctctagcct
     1981 tttggcggtc ttctccaccc tctcgggatc aaagtccctc tcatccacca aaaaggagac
     2041 gatctcttct gacacgggct ttttcagggc gatcttatag tcatcagtga ccggaggatg
     2101 gaggaagaac tccctgatct tctcgaagtc ctcgatctta tcatctctgt ccgccaggat
     2161 gctctccaga tctcctttct cccgaatgag ctttagagcg gtcttgggcc ccaccctctc
     2221 caggccggaa ttgaaatcag tgccgcacat gatgccgatc tccaccagct gcttgtgggt
     2281 gattccaagg tttgccagtc cctcttccag gttgatcacc tccggctcca catccacata
     2341 aatgttcttt cgaggcagct ttctcttgcc ggtgatggcc agattgcgca ccaccagggg
     2401 agcgccaaag agaagagagt cgtagtcctg gctgccggca tagtccacgt cgcccttggc
     2461 ggccatataa gccgcctggg cctcgccctc tgatggggcc tggattaccg gcagccccat
     2521 ggccaggatg agccttctcc cgtcctcgat tatctcggag ttgatccgtg aagccgcctg
     2581 agcatatttg aatccgtctt caccggctgc gcgagcctcc tcccaggccg acgccgcttt
     2641 atcccttatc tccgacctct cggccagggt gacagccttg aaggttgggg cctcgccatc
     2701 gaagacgaag gctaccttca cccctgcctc aatcaggttt gtggtgcgat acaataggcc
     2761 agagagatga gaggtcaccc ttccctggcg gtccatgaga ggggtaccat ccctctgccg
     2821 gatgatggat aaaaactgat agagggtgtt gaaggcatct atcgccaccc atctgccgga
     2881 cagatcctta atctctatct gctttctctt tagcagctcc cctaaatcaa cgcccatgat
     2941 tgtcacatac cactgctcta tgataaatcc tctatcctat gctccgattt ccaggctcca
     3001 attgcagatg catgctacct ttaaatatca gttttgtgca gataacattt ggagtgctat
     3061 tcatgattga gattacaaaa gaagcggctg atatgcttaa aagcaaggtg gtggaggata
     3121 aggtcataag gatgttcctg gcggcagtcg atccatctgg cgcgaactat ggcatgac
//
LOCUS       DAOSGP010000157         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_28681_length_3174_cov_18.892273, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000157 DAOSGP010000000
VERSION     DAOSGP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28681_length_3174_cov_18.892273"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLV49_05190"
     CDS             <1..534
                     /locus_tag="PLV49_05190"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HOG98274.1"
                     /translation="FDDPGCYILDLQMDTTFFDPASFGAAGKKSLVEKYGRVLMPYQS
                     GEGYDWSSRKGMEIVPETDPYQLKSGEDFRARVFYNGKPVPGSYSAIITRSPEDVLVV
                     QHAQESEISGESEDGIISFATGRAGLWVLAYEATIDESGTWTAVKDDPSENYKKGDQI
                     EYDEVAPTAYLTFWVND"
     gene            543..1172
                     /gene="cbiM"
                     /locus_tag="PLV49_05195"
     CDS             543..1172
                     /gene="cbiM"
                     /locus_tag="PLV49_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HOG98275.1"
                     /translation="MVHISDGVLPPAMLAAGFLIALTLLVFSMRNIRIEEIPKVSLIT
                     AALFVASLVHFPIGPTSVHLIMNGLAGILLGRRAFIGVFVALTLQAVFFQHGGLSVLG
                     VNAFNIGVPALLAWQLFEKRRGLKSPRLEAVFGALAGGLAVLASVLLVSLELLALGEA
                     FNEISLLVIGAHLPVMIIEALVVGAAAGFLLKVKPEMLADSSSRIMEVN"
     assembly_gap    1277..1376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1547..2296
                     /gene="cbiQ"
                     /locus_tag="PLV49_05200"
     CDS             1547..2296
                     /gene="cbiQ"
                     /locus_tag="PLV49_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOG98276.1"
                     /translation="MSSFDPERYSDLDSPLHRWETQVKLISLFILLISIVIAVDISQA
                     LAGLIVSMLLVLISGLPLHHVLRFMKWPFLFLLPLLVILPITAGGESLFRYHILTVSH
                     QGLQLGVLFMVRGIAAALLALIIVGTASFDMNINALRSLGMPGPLTQIFLFAYRYIFL
                     FNDDLQTMRRSLASKGFEMRSSARSARILAMAVAMLLIRSYERSEDVFNAMLSRGYQG
                     RLPSGQKMMIKGDDLLKGLLVVAAALFIQFV"
     gene            2335..3165
                     /locus_tag="PLV49_05205"
     CDS             2335..3165
                     /locus_tag="PLV49_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG98277.1"
                     /translation="MNQKMAIEVENLSYSYSDGTRALNEVSFSLAEGESLALLGPNGA
                     GKSTLLLHLNGLLRGQGKVRIRNKEISDESLRWVRSQVGMVFQDPDDQLFMSRLEEDV
                     AFGPTNMELSQKEVDERVQWALKCVGLSDQAAKMPHHLSFGQRKRAAFATVLSMRPPV
                     LVLDEPTSNLDPRSRMEMVSLIREQQRNGTAIITATHDVNLVPLLADRILLLNRSIVA
                     EGSVHQIMSRRDLMDDLGLEMPILADLFETLQEEGLYSGAIPFKKDEAVGAIREMLHR
                     "
BASE COUNT          694 a          781 c          874 g          725 t
ORIGIN      
        1 ttcgatgatc ctggctgcta tatattggac ctgcagatgg atacaacctt ctttgacccc
       61 gcctcgtttg gagcagcggg caaaaagagc ctggtggaga agtacggccg ggtcctcatg
      121 ccataccagt cgggcgaggg ctatgactgg tccagcagaa aaggcatgga gatcgttccc
      181 gaaactgatc cctaccagct caaatccggt gaagacttcc gggcccgcgt gttctacaac
      241 ggcaagcccg tgcctggcag ctacagcgca atcatcaccc gctctcctga ggacgtcctg
      301 gtggtccagc atgcccagga gagcgagatc tcaggagaga gcgaggacgg catcatcagc
      361 tttgccaccg gaagagcggg attgtgggtg ctggcatatg aggccaccat agatgaaagc
      421 ggaacctgga ctgcagttaa ggacgatcca tctgaaaact acaagaaagg agaccagatc
      481 gagtacgatg aggtcgctcc caccgcatac ctcaccttct gggtgaacga ttgaggcgat
      541 agatggtcca tatatcagat ggcgtcctgc ccccggccat gctggcggca ggattcctca
      601 tagctctcac cttgcttgtt ttctctatga gaaacatacg gatagaggag attcccaagg
      661 tctcattgat cactgcagcg ctgttcgtgg ccagcctggt ccacttcccc ataggcccaa
      721 catcagtgca cctgatcatg aacggtctcg ctggcatact gctgggacga agggccttta
      781 tcggcgtttt tgtggccctg actctgcagg cggtcttctt tcagcatgga ggcctgtccg
      841 tgctgggggt caatgcattc aatataggcg ttccagctct gctcgcctgg cagctatttg
      901 agaagagaag aggtttaaag tcccctcgtc tggaggcggt cttcggggct ctggccggag
      961 gactggcagt gctggcttca gtgctactgg tctccctgga gctattggct ctgggcgagg
     1021 cattcaacga gatatcgctc ctggtgatag gcgctcacct gccggtaatg atcatagagg
     1081 ccctggttgt gggagcagca gcaggatttc tcctgaaggt caagccagaa atgcttgcgg
     1141 atagcagcag taggattatg gaggtaaatt gatatgaggt ggatactagc tgtgattatt
     1201 gccgccctgc tcctggcagg agcggccagt gctcacagga tgtttgtggg ccagcagata
     1261 accctggacc ttttcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtga
     1381 gctactctgc cggacataag gcagagctgg agcttgacct ggagcagaag gaagcggaag
     1441 agcagatctc acggccactc cgagcagcgg caggccttgg ctatctgctg gggatcgcag
     1501 gattgtcaat gatattcata tccagaaaga gaagctagaa gaaagcatga gcagcttcga
     1561 cccggaaagg tactctgacc tcgattctcc cttgcaccgc tgggagacgc aagtcaagct
     1621 catatctctt tttatattgc tcatctccat tgtaattgcg gtagatatca gtcaggccct
     1681 ggcagggctg atagtctcta tgctattggt cctgatttca gggcttcctt tgcatcatgt
     1741 cctgagattc atgaagtggc ctttcctatt ccttctcccc ttgctggtca tccttcccat
     1801 caccgctgga ggggagagcc tgtttagata ccatattctg acagtcagcc atcagggtct
     1861 gcaactcgga gtgctgttta tggtccgggg aattgccgcc gccctcctgg ccctgattat
     1921 agtgggaaca gcttcattcg atatgaacat caatgccctg aggagccttg gcatgcccgg
     1981 ccctctcact cagatcttcc tattcgccta tcgctatatc ttcctcttca acgatgatct
     2041 gcagaccatg agaaggtctc ttgcctccaa gggatttgag atgaggtcat ctgccagatc
     2101 cgctcgcatc ctggcgatgg ctgtggcaat gcttctcatc aggagctatg aacgatcgga
     2161 ggatgtcttc aatgccatgc tctctcgtgg ctaccaggga agattgccct ctggccagaa
     2221 gatgatgatc aaaggcgatg atctgctcaa gggcctttta gtagtggctg cagctctctt
     2281 tatacaattc gtctaatgac gttcatttga tcggcgatat tggcaggtat tacgatgaat
     2341 caaaaaatgg ccatagaggt tgaaaacctc agctacagct actctgatgg aacgcgtgcc
     2401 ctgaatgaag tcagcttctc cctggctgag ggagagtccc tggcccttct tgggcccaat
     2461 ggagcgggca agtccaccct gctgcttcac ttgaatggac tattgagggg gcagggaaag
     2521 gtcaggatcc ggaataaaga gataagcgat gagagcctga gatgggttcg aagccaggtg
     2581 ggaatggtct ttcaggaccc ggatgatcag ctcttcatgt cccgtctgga ggaggatgtg
     2641 gccttcggtc cgacgaatat ggaattatcc caaaaggagg tggacgagag agtgcagtgg
     2701 gcactgaaat gcgttggcct ttccgaccag gcggcaaaga tgcctcatca cctgagcttc
     2761 ggccagagga agagagcggc ttttgccacc gtcctgtcca tgaggcctcc agtgttggtc
     2821 ctggatgaac ccacctcgaa cctggatccc cgatccagga tggagatggt ctcacttatc
     2881 cgagagcagc agaggaatgg gacggccatc atcaccgcta cccatgatgt aaaccttgta
     2941 cccctgctag cggacaggat tcttctcctc aataggagca ttgtggcaga gggcagtgtt
     3001 catcagatca tgagcaggag ggatttgatg gatgatctgg gcctggagat gcccatcctt
     3061 gcagatctat tcgagactct gcaggaggag ggcctgtatt ccggagccat tcccttcaaa
     3121 aaagatgagg ccgttggagc tataagagag atgctccatc gatgagatag gcag
//
LOCUS       DAOSGP010000158         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_29476_length_3113_cov_17.974166, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000158 DAOSGP010000000
VERSION     DAOSGP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29476_length_3113_cov_17.974166"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PLV49_05210"
     CDS             complement(<1..1389)
                     /locus_tag="PLV49_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOG98278.1"
                     /translation="MTDIFGNSSSSNSVSDHSPFKQAVGIDDLAVYIPQLYLPLEGEF
                     SRRREIDPGKLTRGIGIERMAIPDAHEDAATMAAMSLLELMRRCDLLPEDIGKIHIGT
                     ESAVDEAKAIGTYVIGMLEKVYGPGSFQHCSTVEFKSACIGTTLALESISYWVAADEG
                     ERVGVVIASDVAKYPLGSSGEYTQGAGSVSLLVKKNPRIAALEQIYGCFTRDENDFFR
                     PTGCTMATVNGKHSNQCYLDAMQGAFASFAAKAARKGIISPKNGECTTDWIDHLLFHI
                     PYPRMIEYASAAIFRQDWKNSSRWSDVEAEIGPEPEAGNYDDLERYRLDLARYARSFA
                     RSRQFLEAYNARVIDSAALSRSIGNIYTGSIYLGLASLLELQKLRAHERLCFGAYGSG
                     CSALVFSARVSPKVSSVPSRDLFERLEKRREISLEEYELLHEGEIKESLIQPSGEFAL
                     AQIDHQGYRHYEY"
     gene            1524..>1723
                     /locus_tag="PLV49_05215"
     CDS             1524..>1723
                     /locus_tag="PLV49_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOG98279.1"
                     /translation="MSGDVYKVIELVGTSTTSWEDAVKTVVDRATKTLRDLRIAEVKE
                     LDVRLDNGKIAEYRAKVRLSFK"
     assembly_gap    1724..1823
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1824..3058)
                     /locus_tag="PLV49_05220"
     CDS             complement(<1824..3058)
                     /locus_tag="PLV49_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG98280.1"
                     /translation="MRSIVKGSVGTIVGETTPTAFKFLISKEVGRGTYIKAKGDGREW
                     ILAQIEDIKRSNTAYTVNQLNDAARNYDSREMMIAESRVIGVESNGKLRLPTSPARPG
                     ESVFLADEKLIKATLGLSKGDMYIGMLKGYDIRVEMDANTLVQKHCSVLAKTGSGKSY
                     TAAVILEELLDRKVALLVIDPHGEYASMKEPNNVGDFSKYKIKPRGYDITVYTPGEMA
                     MNPRADRPFRFNSLNLSAREVAKMIPNESSSSGQLGLLYEAISALRAETDAYTLEDII
                     EQVVRSNSKVKWNLVGQLESLLELGLFSGTATPCDELLRPGRAAVIDMTGIIPELQAM
                     IVARLLTDIFEARKRRLISPGMVVVEEAHNYIPERGTGNAASTNIVRTIAAEGRKFGL
                     GLMIISQRPARVDKNVISQ"
BASE COUNT          672 a          865 c          747 g          729 t
ORIGIN      
        1 atactcataa tgccggtatc cctggtggtc gatctgggcc agagcgaact cccctgaagg
       61 ctggatcagg ctctccttta tctctccctc atggagcaat tcatactcct ccagggatat
      121 ttcccttctc ttctccagcc tctcaaacag gtcccttgac ggtactgaag agacctttgg
      181 gctgacccgc gccgagaaga ccagggctga acatccgctt ccgtaagccc caaagcaaag
      241 cctctcatgc gctcgaagct tctgcaactc caggaggctg gccaggccca gatagatcga
      301 accggtatag atattcccaa tggaacgaga gagagctgcc gaatctataa ccctggcatt
      361 ataagcctcc agaaactgcc tggaccgagc aaagctccgg gcatagcggg ccaggtcgag
      421 ccgatacctc tcgagatcat cataatttcc agcctcgggc tccggtccga tctccgcctc
      481 aacatcactc caccggctgc tgttcttcca gtcttgccgg aatatagcgg cagatgcata
      541 ctcgatcatt ctgggataag ggatatggaa gagaaggtgg tcgatccagt cggtggtgca
      601 ctccccattc tttggactga ttatgccctt gcgagcagct ttggcagcaa atgatgcaaa
      661 tgctccctgc atggcatcga ggtaacactg gttgctatgc ttgccattga ccgtggccat
      721 tgtgcagcct gtcgggcgga agaagtcgtt ttcatcccga gtaaagcagc catagatctg
      781 ctctaaagcg gcaatcctcg gatttttctt gaccagcaat gagacgcttc ctgccccctg
      841 agtgtattct ccagaggagc ccagtgggta tttggccaca tcagaggcta tgaccacccc
      901 aaccctttcg ccctcatcgg cagccaccca gtagctgatg ctctccagag ctaaagtggt
      961 gccgatgcag gccgacttga actccaccgt ggagcagtgc tgaaaagagc cgggaccata
     1021 taccttctcc agcatgccga tgacataggt tccaatcgct ttggcctcgt cgaccgcaga
     1081 ctccgtgcca atatgaattt ttccaatatc ctcgggcaat aggtcgcagc ggcgcatcag
     1141 ttcgagaagt gacatggcgg ccatggtggc tgcgtcctca tgggcatcgg gaatggccat
     1201 cctctctatt cctattcctc tggtgagctt gcccggatcg atctctcgcc ggcgggagaa
     1261 ctccccctct aaaggaaggt aaagctgcgg aatatagacg gccaggtcgt caattcccac
     1321 cgcctgcttg aatggcgaat ggtctgaaac tgaattgctg ctcgaagaat tgccaaaaat
     1381 atcggtcact atagcctctc tccttttggc tagaggctgg gtggatatta tttcaggctt
     1441 ttgctgctat taatcgagaa agatcaggaa aatatatcaa ctaaaagagc caataattga
     1501 ttaaaattta aggagtgaag gatatgtccg gagatgtata caaagtaata gagctagttg
     1561 ggacctcgac caccagctgg gaagatgccg tcaagacagt agtcgacagg gcaaccaaga
     1621 ccctcagaga ccttcgcatc gctgaggtca aggagctgga tgtcaggctg gataatggga
     1681 agatcgccga gtacagggcg aaggtccgcc tctcgttcaa gtannnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnncactgag atatgacgtt cttgtccacc ctggccggcc
     1861 tctggctgat gatcatcagt cccaggccga acttgcgccc ttcagcggca atggtgcgca
     1921 cgatattggt ggatgccgca tttcctgtcc cccgctcggg aatatagtta tgcgcctctt
     1981 ccaccaccac cattccagga gagatcagcc tgcgctttcg ggcctcgaag atatcggtca
     2041 gcagacgggc gacgatcatg gcctgaagct caggtatgat cccagtcata tcgataactg
     2101 ccgccctccc aggcctgagc aactcgtcgc atggcgtcgc cgtaccggag aagagcccca
     2161 gctccaggag agactccagc tggcccacca gattccactt caccttggag ttgctcctca
     2221 ccacctgctc tatgatatcc tccagggtgt aggcatccgt ctccgctctc aaggcgctga
     2281 tggcctcata gagcagtccc agctggccgc tgctgctgct ctcattggga atcatcttcg
     2341 ccacctctct tgccgagagg ttcaatgaat tgaagcgaaa tggcctgtcc gccctgggat
     2401 tcatggccat ctcccctgga gtatagacag tgatatcata tccccggggc ttgatcttat
     2461 acttgctgaa atcgcccacg ttgttcggct ctttcatgga ggcgtactcc ccatgaggat
     2521 cgatcaccag cagtgccacc ttcctgtcca ggagttcttc aagtatcaca gctgctgtat
     2581 aggattttcc cgaaccggtc tttgccagaa cgctgcagtg cttttgcacc agggtattgg
     2641 catccatctc cacccggata tcgtagcctt tcagcatgcc tatgtacatg tcgcctttag
     2701 ataggccgag ggttgccttg atcagcttct catcggccaa gaataccgac tctccagggc
     2761 gggctgggct ggtggggagg cgcagcttgc cgttgctttc cacgccaatc accctcgact
     2821 cagcgatcat catctctctg gagtcgtagt tcctggctgc atcattgagc tggtttacgg
     2881 tgtaagcagt gttagacctt tttatgtcct caatctgggc taagatccac tccctgccat
     2941 ctcctttggc ttttatgtat gtgccccgtc caacctcttt acttatgaga aacttaaatg
     3001 cagtaggtgt ggtctcacca acgattgtgc ctacagatcc cttgactatc gacctcaaag
     3061 accatcaccg cattcttgcg cttccatgcg caacacattc ttcaaaaatt ttc
//
LOCUS       DAOSGP010000159         3109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_29547_length_3109_cov_20.008186, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000159 DAOSGP010000000
VERSION     DAOSGP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29547_length_3109_cov_20.008186"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="thiM"
                     /locus_tag="PLV49_05225"
     CDS             complement(<1..677)
                     /gene="thiM"
                     /locus_tag="PLV49_05225"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOG98281.1"
                     /translation="MILDAAEVLERVRRDKPLVCHLTNFVTIYDCANIVKVFGASPVM
                     AHAREEVADMAKIASSLVLNIGTLTTEFVEAMLLAGISANEKKIPVVLDVCGAGATRF
                     RDDKCQEILDAVEVSIIKGNSSEVARIAGEDVKTRGVDAADIESNLLGVAHSLAGERG
                     CTVVITGKDDIVADMKRAVWVHNGHPMMANVVGTGCMATSVIGAFAAVEADHVVASVS
                     GLVCYEVA"
     gene            complement(708..2285)
                     /gene="serA"
                     /locus_tag="PLV49_05230"
     CDS             complement(708..2285)
                     /gene="serA"
                     /locus_tag="PLV49_05230"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOG98282.1"
                     /translation="MKVLVSDSLAEDGVKRLQSGADVDVITNLSPEELIQKIPDYDAL
                     VIRSGTKVTADVINAAKRLRVIGRAGVGIDNVDVEAATKKGIIVLNTPGGNTISAAEH
                     TIAMMLALARNIPQANSALHQGEWNRKKYTGVEFFNKTLGVVGLGRVGAEVAARMKSF
                     GMRILAYDPFVTEEKAQQMGLTLATLETVLREGDFITVHTPLTNETRNLIDDDEFKIM
                     KDGVRIVNCARGGIINEAALAKAVAEGKVAGAAVDVFTKEPPTGNPLLGQERIITTPH
                     LGASTAEAQVNVALAVADQILAIAKGGLPTNAINMPAISPETLAVMEPYMMLAERMGS
                     LLGQMVGSGFESLELIYSGTIAEKDTRPVTISAIRGLLGCLMGKDSINFVNATTTLKE
                     MGVKLLESKTESINGYSNAITMKLSKNGVINMVQGTVSGNKEKRIVQLDLYRTYIPTE
                     GDMVIAVIDDKPNIIGPCCVVLGEGNINIGSMHVGRMDEGQPQLMVLSVDQMVPDDLM
                     KRLLQVPGVKSAKMVEL"
     gene            complement(2368..3060)
                     /locus_tag="PLV49_05235"
     CDS             complement(2368..3060)
                     /locus_tag="PLV49_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOG98283.1"
                     /translation="MDVLAINGSPHMNEGNTAKILNPFLEGLKEAGANVELIYSWKLN
                     IEPCSGDMSCWFVNPGTCAKDDDMKTILPKIKNADVIVWASPVYYSGITGPLKTLMDR
                     QLPAYVMGQPRSKMQKAVLVSTCSAWEISMFDPLLVQMKAIYDNPQEGCEFAGALLRP
                     MAEGMNEMIKAGETALIEGIFKAAKQAGHQLIKDGRISEDMEKAVSRELMPRDAYYRA
                     AQEMIEQMRTSQ"
BASE COUNT          623 a          911 c          767 g          808 t
ORIGIN      
        1 gccacctcat agcagaccag cccggagacg gaagcaacga catgatccgc ttcgacggcc
       61 gcaaatgctc caatcaccga tgtggccatg caccctgtgc ccactacatt ggccatcatg
      121 gggtggccgt tatgcaccca taccgctctc ttcatatcgg ccacgatatc atccttgccg
      181 gtgatgacca cagtgcaacc tcgctccccg gcaagagaat gggccacccc cagcaggttt
      241 gattcgatat ctgcggcatc aacgcccctg gttttgacat cctctccagc gatgcgggcg
      301 acctcggacg agttgccttt gatgatgctt acttcgactg catctaagat ctcctggcat
      361 ttgtcgtccc ggaacctcgt cgctccggcg ccgcagacat caaggaccac gggtatcttt
      421 ttttcattgg cgctgattcc ggcaagcagc atggcctcaa cgaattccgt ggtgagggtg
      481 ccgatattca ggaccagaga agaagctatc tttgccatat cagctacctc ttccctggca
      541 tgtgccatta ccggagaggc cccaaagacc ttgacgatat tggcgcaatc gtaaatggtg
      601 acgaagttcg tcaggtggca taccaggggc ttatcccttc tcaccctctc aagaacctct
      661 gctgcatcta atatcatccg acatctcctt tcttgctttc gtcggcttta cagctccacc
      721 atcttggcgc tcttcacccc tggaacctgc aatagccttt tcatcaaatc atcgggtacc
      781 atctggtcga cgctcagtac catgagctga ggctgaccct cgtccatacg ccccacatgc
      841 attgagccga tgttgatatt gccctcaccc aaaaccacgc agcagggccc aatgatattc
      901 ggcttgtcat ctatgactgc tatcaccatg tcaccctctg tggggatata ggtccggtag
      961 agatcaagct gcacaatgcg cttctccttg tttcctgaga ccgtgccctg aaccatgttg
     1021 atcacgccat tcttggaaag cttcatggtt atggcattac tgtatccatt tatcgactcg
     1081 gtcttgctct ccagaagctt cacgcccatc tctttgagcg tcgtggtggc attgacaaag
     1141 ttgatgctgt cttttcccat caggcagccc aaaagacccc tgatggctga tatggtcacg
     1201 ggtctcgtat ccttctcggc aatggttccg ctatagatca actccaagct ctcaaagccg
     1261 ctgccaacca tctgcccgag cagcgatccc atcctctcgg ccagcatcat atagggctcc
     1321 atgaccgcca gcgtttccgg tgatatggca ggcatgttga tggcattggt gggcagtccg
     1381 cctttagcta tggccaggat ctggtctgcc actgctaagg cgacattgac ctgggcctcg
     1441 gcggtagaag ctcccaaatg gggagtggtt atgatcctct cctgcccgag cagggggttt
     1501 ccggtgggag gttccttggt gaagacatcg accgccgctc cggcaacctt tccttcggcc
     1561 acagctttgg caagagcggc ctcgttgatg atcccgcctc gagcgcagtt cacaatgcga
     1621 accccgtcct tcatgatctt gaactcgtca tcgtcgatca ggttgcgggt ctcattggta
     1681 aggggagtat ggacggtgat gaagtcgccc tcacgcagaa cggtctccag ggtcgccagg
     1741 gtcagtccca tctgctgggc cttttcttct gtcacaaagg ggtcataggc taagattcgc
     1801 atgccaaagg acttcatgcg tgctgcaacc tctgctccaa cccggcccag accgacgacg
     1861 cccagggtct tgttgaagaa ctcgacgcct gtgtacttct tgcgattcca ttcgccctgg
     1921 tgcagggcgg agtttgcctg gggtatgttc ctggccaggg ccagcatcat ggcgatggtg
     1981 tgctcggctg cggagatggt atttccgccg ggggtattga gaacgatgat cccctttttg
     2041 gtcgcagcct ctacgtccac attgtctatg cccacaccgg ctctgcctat tactctgagc
     2101 cttttcgcgg cattgatgac atcagccgtt accttggttc cgctgcggat taccagggca
     2161 tcgtagtctg ggatcttctg gatgagctcc tctggagaga ggttggtgat aacatcgaca
     2221 tcagcacccg actgcagtct cttcacgcca tcctctgcta atgaatcact gacaagaacc
     2281 ttcatgatca taccttcttc ctcgggggct aaagacgtac aagcatttga tcctttcgag
     2341 agcacacttc agaagggtat tagcaatcta ttggctcgtc ctcatctgtt cgatcatctc
     2401 ttgagcagct ctgtagtatg catccctggg catcagctct ctgctcaccg ccttctccat
     2461 atcctctgag atcctgccgt cttttatcag ctggtggcca gcctgctttg ctgccttgaa
     2521 gatgccttcg atcagtgcgg tctcaccggc ttttatcatc tcattcatgc cctctgccat
     2581 gggcctgagc agcgcgccgg caaactcgca gccctcttgt gggttgtcgt agatggcctt
     2641 catctgcaca agaagcggat cgaacatgga aatctcccag gcggagcagg tggagacgag
     2701 aaccgctttt tgcatctttg atctgggttg gcccatcaca taggccggta gctgtctgtc
     2761 catcagagtc ttaagagggc cggtaattcc cgaataatag accggactgg cccagacgat
     2821 gacatctgca tttttgatct ttgggaggat ggtcttcata tcatcgtctt tggcgcaggt
     2881 gccgggattc acaaaccagc agctcatgtc tccgctgcag ggctctatgt tcagcttcca
     2941 agaatagatc agctcaacat ttgctcccgc ttccttcaag ccttccagga atggattcaa
     3001 gatcttcgcc gtatttccct cgttcatgtg agggcttccg tttatggcca ggacgtccat
     3061 tttatatcac actgatattt gtattggcca attgcttatg tacatatct
//
LOCUS       DAOSGP010000160         3076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_29987_length_3076_cov_17.748428, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000160 DAOSGP010000000
VERSION     DAOSGP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29987_length_3076_cov_17.748428"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="PLV49_05240"
     CDS             complement(<1..170)
                     /locus_tag="PLV49_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG98284.1"
                     /translation="MILLQIENLSKTYRLNDRDIEALKAISFTAQPGEILGIVGKSGG
                     GKSTLMKILRGM"
     gene            complement(176..>1405)
                     /gene="metG"
                     /locus_tag="PLV49_05245"
     CDS             complement(176..>1405)
                     /gene="metG"
                     /locus_tag="PLV49_05245"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOG98285.1"
                     /translation="EEWCARSGRDWEDYWKKPSQIIHFIGGDIVYHHCIFWPAMLKGA
                     GYTLPSAVVASGMLKIDDKKFSKSRGNVIWVKDDYMDRGLHPDLLRYYLASYTAHNKE
                     VNFAWNIFADKVNSELVGAFGNFINRALTFSVKNFEGKVPEGKIDADVLARIEETMAE
                     VVSGLEEYEFKKATDSVMTLADYGNTYFQSHEPWKLVKSDKDAAGSVLRSCLQIAKAL
                     VILMEPVMPAKMQNAWKQLGMPGEVSKIALEDALVPLDSGQSLGTPEILFDRLEEKFV
                     KELEKIFMDRIKEAGSKGKGTQDKKKEEISFEDFQSMDIRIGEIKNAEAIKGSNKLLK
                     LAVDIGGEVRQVVAGIAQSYAKEDLVGTQVVVLVNLKPAKLFGIESQAMLLAADVEGK
                     AVLLRPRDAVETGTKVR"
     assembly_gap    1406..1505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1506..1948)
                     /locus_tag="PLV49_05250"
     CDS             complement(<1506..1948)
                     /locus_tag="PLV49_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG98286.1"
                     /translation="MPILVTCGLPYANGPCHLGHLRTYVPADIFVRGLRKMGKEVLFV
                     CGSDSHGTPIVVNAEAQGMTPKALSIKYHQHFDEIFQGMEVDFDFFGDTDDPACHNRT
                     KEMVEALMENGYVYPREIDLAYCPKCERFLPDRYVEGECPYCGKPA"
     gene            2169..>3076
                     /locus_tag="PLV49_05255"
     CDS             2169..>3076
                     /locus_tag="PLV49_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98287.1"
                     /translation="MNKMCLIIPAFLLALAMGPAGAYPLQASDGNATVVLFGSIRMPY
                     ELNDSEEVLKVDTGLLNASNATYQMVDQYDAVYDPAMYKPLNEGRQLVYFVVPKDSLF
                     KLITATPEGGRPININWWATPKAGNEFLLIRYYGITDWLINPDEQGIVVQVRGQNNGT
                     QDVTFSPENFVLLDQWGWPYSATAGFDTEVIAPGNASQERVLVGFSGISLQSRPVALV
                     YGYYTNSPIVIDFDSAYTQLTNEQVYGTAGAQNVSAAQPAGQAVQPIALAAATTNESA
                     VAGAAANNTTAQNATVASNINSSVSST"
BASE COUNT          681 a          795 c          776 g          724 t
ORIGIN      
        1 tccatgcctc gcaggatctt catcagggta ctctttccgc ctccgctctt gcccactatt
       61 cccaaaatct cgcccggctg ggcggtgaag cttatggctt taagggcttc gatatccctg
      121 tcatttaacc tgtaagtttt ggaaagattc tctatctgca gcaaaatcat acctcctaac
      181 gtacttttgt tcccgtttcc acagcgtctc tcggcctgag aagcactgcc ttgccttcca
      241 catcagccgc caggagcatg gcctgtgact ctattccgaa gagcttggcc ggctttaaat
      301 tgaccaaaac cactacctgt gtgcccacaa ggtcctcttt tgcataggac tgggcgatgc
      361 ctgctaccac ctggcggacc tctcccccta tatccacagc aagcttgaga agtttgtttg
      421 agcctttaat ggcctctgca ttcttgatct cccctattct tatatccatg gactggaaat
      481 cctcaaagga gatctcttct ttcttcttat cttgcgttcc cttgcctttg gatccggcct
      541 cttttatcct atccatgaag atcttctcca gttctttgac gaatttctcc tccaggcggt
      601 caaagaggat ctcaggggtt cccaggctct ggccggagtc caggggcaca agagcatctt
      661 ctaaagcgat cttactgacc tctccgggca tgcccagctg cttccaggcg ttttgcatct
      721 ttgcgggcat taccggttcc atcagaatta ccagcgcttt ggcgatctgc aggcagcttc
      781 gaaggacaga gcctgcggca tccttgtcgc ttttgaccag cttccagggc tcatggctct
      841 ggaagtaggt gttcccataa tcggccaggg tcatcacgct gtctgtggcc ttcttgaact
      901 cgtactcctc caggccggat acaacctcag ccatggtctc ttcgatccgg gccaaaacat
      961 cagcatcgat cttgccctca ggcacctttc cctcgaagtt tttcacgctg aaggtgaggg
     1021 cccggttgat gaagttgcca aaagccccca caagctcgga gttgacctta tcggcaaata
     1081 tgttccaggc gaagttgacc tctttattat gagcggtata gctggccagg taatagcgca
     1141 gaaggtcggg atgaaggcct cggtccatgt agtcgtcctt cacccagatg acattgcccc
     1201 gggatttgga gaacttcttg tcatcgatct tgagcatgcc gctggcaact accgccgagg
     1261 gcagggtgta tcctgctcct ttgagcatcg ccggccagaa gatgcagtga tgatagacga
     1321 tatctccgcc gatgaagtga atgatctggg aaggtttttt ccagtagtcc tcccagtccc
     1381 ggccagacct ggcgcaccac tcctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnngcggg cttgccgcag tagggacact ccccttccac atatctatcc ggcaggaagc
     1561 gctcgcattt ggggcagtag gcaagatcga tctccctggg atagacgtag ccgttctcca
     1621 tgagagcttc taccatctct ttggtgcgat tgtggcaagc gggatcgtca gtatcgccaa
     1681 agaagtcgaa atcaacctcc attccctgga agatctcatc gaagtgctgg tgatacttga
     1741 tggacagagc cttgggggtc attccctgag cctcggcatt gaccacgatc ggggttccat
     1801 gggaatccga gccgcagacg aagagcacct ctttgcccat ctttctcaat cctcggacga
     1861 agatgtccgc tggtacatag gttcttaggt ggcccaggtg acagggcccg ttggcgtaag
     1921 ggaggccgca ggttacaagg ataggcattg acaattgaga acaccgccgc tggatatgaa
     1981 agaacaggga gatataggtt tcccaatagc tctcgggggc atggtacagg gatcctggtc
     2041 ggaaggatcc tggataaaat gtatataacc ggatgataaa atacagatga tggcagaatg
     2101 caatttaaat gcgaaaagca tgaagtcagc cattccatga gagatgatgg caaaagtggg
     2161 gatattcaat gaataagatg tgtttaatta ttccggcctt cttactggca ctggctatgg
     2221 gcccggcagg agcctaccct ctgcaggcca gcgatggaaa tgctacggta gtcctgtttg
     2281 ggtcaatcag gatgccatat gagttaaatg atagtgagga ggtcctgaag gtggataccg
     2341 ggcttctgaa cgcctcgaat gccacatatc aaatggtgga tcagtatgat gctgtttacg
     2401 atccagcgat gtacaaaccc ctgaacgagg gaaggcagct ggtctacttc gtggttccaa
     2461 aggacagcct cttcaagttg attactgcga ctcctgaggg cggaaggccg atcaacatca
     2521 actggtgggc tacaccaaaa gccggcaatg agtttctgct gataaggtac tatgggatca
     2581 cagactggct gataaatccc gacgagcagg gaatagtggt tcaggtccgt gggcagaaca
     2641 atggcaccca ggatgtgaca ttcagtccag agaatttcgt tcttcttgat cagtggggct
     2701 ggccgtatag tgcgaccgca ggattcgata ccgaggtcat agctcctgga aacgcaagcc
     2761 aggaaagggt gctggtgggc ttcagcggca tctctctgca gagcaggccc gtggctctgg
     2821 tgtatggcta ctataccaac agtcccatag tcattgactt tgatagtgct tacacccagc
     2881 tcacaaatga gcaggtctat gggacggcgg gagcacaaaa tgtctccgca gctcagcctg
     2941 ccgggcaagc agtccagccg attgcattgg ccgctgcaac gacaaacgag agtgctgtag
     3001 ctggagctgc ggccaacaac accaccgccc agaacgcaac cgtggcctct aacatcaatt
     3061 ccagcgtcag ctccac
//
LOCUS       DAOSGP010000161         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_30190_length_3062_cov_16.471566, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000161 DAOSGP010000000
VERSION     DAOSGP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30190_length_3062_cov_16.471566"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..91
                     /locus_tag="PLV49_05260"
     CDS             14..91
                     /locus_tag="PLV49_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98288.1"
                     /translation="MRCPYCGHRILMKERPTTVKKMNAV"
     gene            91..501
                     /locus_tag="PLV49_05265"
     CDS             91..501
                     /locus_tag="PLV49_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98289.1"
                     /translation="MLVTTSRDPSPKARRFGRALAEFLSLPYLNRGKHSSGGEETWLV
                     VVEDHGNPHGLAKRSIAGEELLAFRLLGEPLAGRLKKDVPVVTGRSTESKAIAQFFEL
                     RWSHLPDPEARTISVASGRIDFMDVGATRFSLRL"
     gene            498..725
                     /locus_tag="PLV49_05270"
     CDS             498..725
                     /locus_tag="PLV49_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HOG98290.1"
                     /translation="MKGWTELVIETPHSQEIYEALAPELNDEIHRSNVELVEGPGFVR
                     LKILGEDVVSLRAALNTWIRLVKIAIEMVSL"
     gene            722..1084
                     /locus_tag="PLV49_05275"
     CDS             722..1084
                     /locus_tag="PLV49_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HOG98291.1"
                     /translation="MSGELPPQIQNQLAQLQQLQQQAQAVMTQKTQIEGLIRETEAAL
                     KELEKSADDAVIYKSVGELLFRADKLKLTEELKERKDMMDIRLKTMAKQEERIQGRFT
                     QLQEQLKQSLGQMPPKGG"
     gene            1095..1169
                     /locus_tag="PLV49_05280"
     tRNA            1095..1169
                     /locus_tag="PLV49_05280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1130..1132,aa:Arg,seq:tct)
     gene            1191..1376
                     /locus_tag="PLV49_05285"
     CDS             1191..1376
                     /locus_tag="PLV49_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HOG98292.1"
                     /translation="MCELTVYTLKGGVREKVMESVVRLMVHDGKVILEGIFGDSMEVE
                     GRISEVNIMSQSATIIG"
     gene            1431..2000
                     /locus_tag="PLV49_05290"
     CDS             1431..2000
                     /locus_tag="PLV49_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98293.1"
                     /translation="MRPIETICVLSISLLLASIMGCLAEDSTGSLEGNPDIPKTMLPD
                     GFKLLAALPEMDSNVNMTDYITEFYGEKEIKPINISVGIYQWEAKEGGQPYDAKVTYI
                     RLPDQEQALAAINNFRSQEDYADQIARGLKIFGNATINDHDALEIRHIQNSNSYKYIY
                     LWNNLDLVVLVEGNNDKNQSLQLASATGL"
     gene            complement(2011..3036)
                     /locus_tag="PLV49_05295"
     CDS             complement(2011..3036)
                     /locus_tag="PLV49_05295"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOG98294.1"
                     /translation="MEHSHGHDLICNKERTTIPMKDRGVAELVGDMGRMGFQGGQLGT
                     SLRVWERMMDEDVTIFLGLAGAMVPAGLGEFIAYLLRERKVDCLVSTGANLFHDLCEG
                     LGIIHFRGSSCADDAYLNECKIDRIYDVFVSEIELHKADNYISDFVKGLDQDRRYSSR
                     ELMEIVGKGLPDTTILGAAHKAGVPIFVPALGDSSWGIGMVIALREGCRVLVDQIKDV
                     DEITRIVERSAQTGVIYIGGGVPKNFIQQTEVIAELIGNYSGGHSYAIQYTADSPHWG
                     GLSGCTFEEAVSWGKVKKEASKVQVFSDATITVPLVVQALQASGHRRKSYPVYNWREG
                     DLELNYR"
BASE COUNT          771 a          793 c          815 g          683 t
ORIGIN      
        1 cgagtacagc ggcatacgct gtccttactg cggccatagg atcctgatga aggagaggcc
       61 caccacggtc aagaagatga acgccgtctg atgctggtca ccacctcgcg agatccttct
      121 cctaaagcga gaaggtttgg gcgggccctg gcggagttcc tctcccttcc ctatctcaac
      181 cggggaaagc atagctcagg aggagaggaa acctggctgg tggtggtgga ggatcatggc
      241 aacccccatg gcctggccaa gcgttctata gcaggagaag aattgcttgc ctttcggctg
      301 ctgggcgagc cgctggcggg acggctgaag aaggatgtgc ctgtggttac gggacggtcg
      361 actgaatcaa aagcgattgc tcagttcttt gagctgaggt ggtcgcatct tcccgatcca
      421 gaggcgagaa cgatttccgt ggcatctggc aggatagatt tcatggatgt aggcgcgacc
      481 aggttcagtc tgaggctatg aagggctgga cagagctggt catagagacg cctcattccc
      541 aggagatcta tgaggcgctg gcaccggagt tgaacgatga gattcaccgg tcgaatgtcg
      601 agttggtcga aggacctggc tttgttcgat tgaagatcct gggcgaggat gtggtctccc
      661 ttcgggcagc cttaaatacc tggataaggt tggtcaagat cgcaattgag atggtgagtt
      721 tatgagtggc gaattgcctc cgcagataca aaaccagttg gctcagcttc agcagctcca
      781 gcagcaggct caggccgtta tgactcagaa gacccagata gagggtctga tcagagagac
      841 cgaagcagcg ctaaaagagc tggagaagag cgcagacgac gctgttatat acaagagcgt
      901 cggcgaactt ctattcagag cggacaaatt gaagctgact gaggaattga aagagagaaa
      961 ggacatgatg gatatcaggc ttaagacgat ggcaaagcaa gaagagcgca ttcaagggcg
     1021 ctttacacag cttcaggaac agcttaagca gtcattaggt cagatgccgc ccaagggcgg
     1081 ctaaacaaac ttgcggaccc gtggcttagc caggatatag caccgggctt ctaacccggg
     1141 ggtcgcgggt tcgaatcccg ccgggtccgt tttatatttt ttgaggatat atgtgcgaac
     1201 tgacagttta taccctgaaa ggtggagtcc gggaaaaggt catggagagc gtggtcagac
     1261 tgatggtcca tgacggcaag gtgatactgg aaggcatatt cggcgattcc atggaagttg
     1321 agggaaggat ctccgaagtg aatataatgt cccaatcggc taccataatc ggatgaagat
     1381 cccaattcgg ctcataaagc ttatggactt gaaaatggat gagaaaagct atgagaccaa
     1441 ttgagaccat ttgtgtgctg tccatatccc tgcttttagc atcgataatg ggctgcctgg
     1501 cagaggatag cactggttcc cttgaaggca atccggatat tccaaaaacg atgctgccgg
     1561 atggattcaa gctgttggct gccctccctg agatggactc caatgtgaat atgaccgatt
     1621 atataaccga gttctatgga gaaaaggaga tcaagccgat caatatctct gtaggaatat
     1681 atcaatggga ggccaaggaa ggcggccagc cctatgatgc caaggtcaca tatatccggt
     1741 tgcctgatca ggaacaggca ctagcagcca tcaataactt caggtcccag gaggattacg
     1801 ccgatcagat cgccagaggc ctcaagatat tcggaaatgc cacaatcaac gaccacgatg
     1861 ctctggagat aagacacatc caaaatagca acagctacaa atatatttat ctctggaaca
     1921 atttagatct ggtggtactg gtcgaaggca acaatgataa aaatcagagc ctgcagcttg
     1981 cttctgccac aggcctctga aaatcttttt ttacctgtag ttcagctcga gatctccctc
     2041 gcgccagttg taaaccggat agctctttct cctatggccc gatgcctgca atgcctgcac
     2101 caccaggggc acagtgatgg tggcatcgga gaagacctgc accttgcttg cctccttttt
     2161 caccttgccc caggagaccg cctcctcaaa tgtgcagcct gataggcccc cccaatgagg
     2221 agagtcagcg gtatactgaa tggcatagct atgccctccg gagtagttgc caatgagctc
     2281 cgctatgacc tcggtctgct ggatgaagtt cttggggacc ccgccaccga tatatatcac
     2341 accagtctgg gcggacctct ccactatcct tgtgatctca tccacatcct tgatctgatc
     2401 gaccaaaacc cggcagcctt cgcgcagggc aataaccatc ccaatgcccc aggagctatc
     2461 tcccagagca ggaacgaata tggggacacc agctttatgg gcagcaccaa ggatggtggt
     2521 atcgggcagc cccttgccca ctatctccat cagctcgcgg gaggagtagc ggcggtcctg
     2581 gtccaggccc ttaacgaaat ccgatatgta gttgtctgcc ttatgcaact cgatctccga
     2641 gacgaataca tcgtatatcc ggtcgatctt gcattcgttc aggtaggcgt cgtcagcaca
     2701 cgagcttccc cgaaagtgaa ttatccccaa tccctcgcac agatcgtgga atagattggc
     2761 cccagtgctt accagacagt caaccttcct ctccctcaat agatatgcaa tgaactcccc
     2821 gagccccgct gggaccatag ctcccgccag tcccaggaag atggttacat cctcgtccat
     2881 catcctctcc cagacgcgca gagatgtgcc aagctggcca ccctgaaagc ccatcctgcc
     2941 catatctcca accagctcag cgacgccccg gtccttcatg gggatcgtcg tcctctcttt
     3001 attgcatatc aagtcatgac cgtgactatg ttccattcta tccctcttca gcctctggtt
     3061 at
//
LOCUS       DAOSGP010000162         3049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_30356_length_3049_cov_18.722779, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000162 DAOSGP010000000
VERSION     DAOSGP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30356_length_3049_cov_18.722779"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLV49_05300"
     CDS             <1..675
                     /locus_tag="PLV49_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOG98295.1"
                     /translation="IRSENVLLPESSTEEEIIAKIEELNLREDINGILLQLPLPEGLN
                     PQRAMMSILPEKDVDGFHPRNMGALLLGAERLVPCTPLGIIYALERLDKKLEGTEVVI
                     VGHSNVVGKPLAAMMLNRNATVQVCHVFTRDLAEHTRDADILVVAAGVPGLIKKEMVQ
                     PGAYIFDVGINRVGDKTVGDVDYEEVSQIAGAITPVPGGVGPLTVAMLLRQTLNATED
                     QTKDQQ"
     gene            732..1328
                     /locus_tag="PLV49_05305"
     CDS             732..1328
                     /locus_tag="PLV49_05305"
                     /inference="COORDINATES: protein motif:HMM:NF021420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2121 domain-containing protein"
                     /protein_id="HOG98296.1"
                     /translation="MSLIVALAKSKEAVIGGDRRSITFLGSWPELEEELYCGRISDDE
                     ALLAKAMEIGATLQVTDGRDKVWRRGDLLVGEVTEITPQLERRRRIYLAPGSQLQVDI
                     TGKEVRIRDRGAMGCIIYGNRFTQQIAVSIVVQANGRVDEALIRKIFQEAAERTASVS
                     QEHVILRSERMFTNPQKALEKALDEDCQENGWKLCDPL"
     gene            1313..1981
                     /locus_tag="PLV49_05310"
     CDS             1313..1981
                     /locus_tag="PLV49_05310"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOG98297.1"
                     /translation="MRSAIILAGGNSHRLGVEKALLEFDGRPLICWVAETLLSAADEL
                     VVVARDQEHAKRLAEIVSDRTLLDQTRSDRIRIRFTWDRVSGFGPVAGLEAGLGMAEG
                     RFAFAAACDLPFLNGMVIQRLFELADPGRGYEAAVPVQPNGYFEPLHSVYEREKMQAS
                     CQRALESGERRIHVPLQELVVNRVSKELLLPMDPELLSFFNLNTREDLEKARTLWQEK
                     GKKG"
     gene            complement(1987..2169)
                     /locus_tag="PLV49_05315"
     CDS             complement(1987..2169)
                     /locus_tag="PLV49_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98298.1"
                     /translation="MPRRMYKLQQPSMSREKNLVLILLAVIVALILWNSFWSLAKLAL
                     FIAIVYMIYYALKRYF"
     gene            2274..>3049
                     /locus_tag="PLV49_05320"
     CDS             2274..>3049
                     /locus_tag="PLV49_05320"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOG98299.1"
                     /translation="MDKLELVMRNTEEIVTVDELKGLLEKPSRPRAYVGYETSGKVHL
                     GHMLTANKLLDLQRAGFDVVVLLADLHAFLNEKGTLEEVRQIADYNRDCFMALGLDPE
                     RTEFVYGTDYQLQPDFMLKILQMARNTSLNRARRSMDEVSRNAENPMVSQMIYPLMQA
                     VDIADLKIDLAVGGIDQRKIHMLAREELPRLGLPAPVCLHTPLIPGLNGEKMSSSKGN
                     NIAVDEPAQDVEKKIKSAFCPAKVVENNPVLAICKYHVFPR"
BASE COUNT          742 a          731 c          943 g          633 t
ORIGIN      
        1 ataagatccg agaacgtgct cttgccggaa agcagcacag aagaggagat catcgccaaa
       61 atagaggagc tcaaccttcg ggaggatata aacggcatac tgcttcagct gcccctacct
      121 gagggactga atcctcagag ggcgatgatg agcattctgc cagagaagga tgtggatgga
      181 tttcatcccc ggaatatggg ggcattgctt ttgggggcgg agaggctggt gccctgcact
      241 cctctgggga tcatctatgc cttagagagg cttgataaga agctggaggg gacggaggtt
      301 gtcattgtcg gccacagcaa tgtggtgggc aagcctcttg cggccatgat gctcaaccgc
      361 aatgctacgg tgcaggtatg ccacgtcttt acccgcgacc tggctgagca cactcgcgat
      421 gcagatatac tggtggtggc agcaggagtt ccgggcctca tcaaaaagga gatggtccag
      481 ccaggagcct atatttttga tgtgggcata aaccgggtag gagacaagac ggtgggagat
      541 gtggactatg aggaggtatc ccagatcgct ggagcgataa caccggttcc aggcggggtt
      601 gggccgctca ccgtggccat gctccttcgg cagaccctga acgccacaga ggatcagaca
      661 aaggaccaac agtaacggaa taggatcaaa aggaataggc atagagaaaa agctcaggaa
      721 aggatcagaa gatgtcactg atagtcgccc tggccaagag caaggaagcg gtgataggag
      781 gcgataggcg ttccatcacc ttcctgggca gctggcccga gctcgaggag gagctctatt
      841 gtggccggat ctcggatgat gaggctcttt tggccaaagc tatggagatt ggggccactc
      901 tgcaggtaac agacggcagg gacaaggtct ggaggcgggg cgaccttttg gtgggggagg
      961 taacagagat caccccccag ctggagaggc gcagaaggat ctacctcgcc cctgggagcc
     1021 agctgcaggt cgacatcacc gggaaggagg tcaggatccg agatcgcgga gctatgggat
     1081 gcatcattta tggaaaccgg ttcacccagc agattgctgt atcgattgtc gttcaggcca
     1141 acggccgggt ggatgaggct cttatccgga agatcttcca ggaggcagca gagagaacgg
     1201 cttctgtcag ccaggagcat gtgatcttaa ggagcgagag gatgttcacc aacccccaaa
     1261 aggctctgga gaaagccctg gatgaggact gccaggagaa tggctggaag ctatgcgatc
     1321 cgctataatc ctggcaggag gaaacagcca tcgcctgggt gtggagaagg ccctcttgga
     1381 gtttgatgga aggccgctca tctgctgggt ggccgagact ctcctttctg ctgccgatga
     1441 gcttgtggtg gtggccagag atcaagagca tgcgaagagg ttagcggaga tcgtatcgga
     1501 tagaactctc ttggatcaga cccgttcaga tcgaatccgg attcgtttca cctgggacag
     1561 agtcagcgga ttcggaccgg tggcggggct tgaagctgga ttggggatgg ccgagggcag
     1621 gttcgccttt gctgccgcat gcgatcttcc ctttctaaac gggatggtaa tccagaggct
     1681 ttttgagctg gcggacccag gaaggggcta tgaggcggca gttcctgtgc agcctaatgg
     1741 atatttcgag cccctccatt ccgtctatga gcgggagaag atgcaagcat cctgccagag
     1801 ggctttggag agcggggaga gaaggattca cgttcccctg caggagctgg tcgtcaaccg
     1861 ggtctcaaaa gagcttctgc tgccgatgga ccccgagctt ctctcttttt tcaacctcaa
     1921 taccagagag gatctggaaa aggcaagaac tctatggcag gagaaaggca aaaaaggctg
     1981 attattttag aaataccgct tcagagcgta atagatcatg tagactatcg ctatgaagag
     2041 tgctaattta gccagagacc agaagctgtt ccagaggatt agggccacta tgacggccag
     2101 cagtatcagc actagattct tctcgcggct cattgatggc tgctgaagtt tgtacatccg
     2161 ccttggcatg aaaggtcatc gtcggaatta gtattaatca tttttcccaa gctcaacctc
     2221 agaaccaaaa gcattataca tccttattcc tttcctgggc tggtgaattt gatttggaca
     2281 agctcgagct ggtgatgagg aatacagaag agatcgtgac cgtagatgaa ttgaaaggac
     2341 tgctggagaa gcccagccgc ccgcgggctt atgtgggcta tgagaccagc ggcaaggtcc
     2401 atttgggcca tatgctgacc gccaacaagc ttctcgacct gcagagggcg ggcttcgatg
     2461 tggtcgttct tttagccgat ctgcatgcct ttctcaatga gaagggaact cttgaggagg
     2521 tgcgccagat cgccgactac aaccgggact gctttatggc tttgggcctc gacccggaga
     2581 ggacggagtt cgtctatggc acagattatc agctccagcc ggatttcatg ctcaagatcc
     2641 tgcagatggc cagaaatacc tctctcaacc gggccaggcg cagcatggac gaggtcagcc
     2701 ggaatgcaga gaaccccatg gtttctcaga tgatatatcc cctgatgcag gcggtcgaca
     2761 tcgccgacct gaagatcgat ctggcggtgg gaggcataga ccagaggaag atccacatgc
     2821 tggcccgtga ggagctgccc cgcctgggac tgcccgcgcc cgtatgtctg catacgcccc
     2881 tgattccagg actgaacggg gagaagatgt cctcctctaa ggggaataat atcgcggtgg
     2941 acgagccggc tcaagatgtg gagaaaaaga tcaagagcgc cttctgcccg gcaaaagtgg
     3001 tggagaacaa tccggtgctg gcgatatgca agtatcacgt cttcccccg
//
LOCUS       DAOSGP010000163         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_30778_length_3019_cov_18.820513, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000163 DAOSGP010000000
VERSION     DAOSGP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30778_length_3019_cov_18.820513"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..1566
                     /locus_tag="PLV49_05325"
     CDS             10..1566
                     /locus_tag="PLV49_05325"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOG98300.1"
                     /translation="MKPLWGIVFYDVHSLVNEKVRIFDTTLRDGEQTPGVSLTTDEKI
                     QIAQQLDKLGVNVIEAGFPVSSKGEFDSVRQIARSGLQAQVCGLSRIVKKDAQACLDA
                     DVDLVHVFSPTSDIQIAHTIKMSHDEIVEGAMRTVEFVKDNGRTCLFSAMDATRTSPE
                     FLHRIFKAVEEVHCDIINIPDTVGVAVPSSFYRLVKDICDDVHMVVDVHCHNDFGLAV
                     ANSLAAVEAGAREVQVCVNGLGERAGNANLAEVVMGLHSIYNAKTSIKTEYLVETARM
                     VERLTKVKIPITAPIVGENAFSHESGIHSHGIIENSKTFEPGIMTPEMVGHKRRIVLG
                     KHTGKHAVAKVLEEAGYRPSDEQLQEILERIKELGDKGKQVGNIDLQTIADVVIGDVA
                     KESQAVVLKEVSVMTGNIITPTATVKALVRGKEKVLANTGVGPVDAAVNAVQGLLDSD
                     TSIHLCDFRIEAISGGADALAEVVIGVEDDRGRRVSARSAREDIVMASVEALVSAINR
                     LMLLEEAAAR"
     gene            1637..2401
                     /locus_tag="PLV49_05330"
     CDS             1637..2401
                     /locus_tag="PLV49_05330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG98301.1"
                     /translation="MTLSGVIDSHCHLDFKHFNKDREAVMENARKAGVVRMINSGVDY
                     STNSKSLELAKNYDFISATLGLSPNSIDGKNDPEIKMILDQIESNARQAVGIGEAGLD
                     YYHTKDAPSRERQAEIFKKVIAIAESLNLPLVIHSRDAEQPALDMVKHLDKVVFHCYS
                     GTLPTMKEAQDRGYYISLATNVCRSGHHQILARNVSLDHLLVETDSPFLSPRKGRNEP
                     ANVLDSVRLIARIKGLEPQEVAAQTATNTKRIYNIR"
     gene            2529..>3019
                     /locus_tag="PLV49_05335"
     CDS             2529..>3019
                     /locus_tag="PLV49_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00288 family NYN domain-containing protein"
                     /protein_id="HOG98302.1"
                     /translation="MDRMTVPFEHIMKYLGSRKEKGRKRIGLLVDGPNMLRKEFQMDL
                     EEIRDILKDYGDIKMGKVFLNQYASEKLVEAVENQGFEPVICTSDVDVRMAVEGVDMI
                     YNPVIDTLALVTRDADLKPVLMKAMEHGKETIIFGAEPGFSVALRNSADYVIVLRDGQ
                     YVT"
BASE COUNT          761 a          753 c          910 g          595 t
ORIGIN      
        1 ctatgacata tgaaacctct gtggggaatc gtcttctacg acgttcattc gcttgttaat
       61 gaaaaagtgc gcatttttga taccaccctt cgcgatggag aacagactcc tggcgtctct
      121 ctcaccacgg atgagaagat ccagatcgcc cagcagcttg acaaactcgg agtgaatgtt
      181 atcgaggccg gttttcccgt ctcctcaaag ggcgagtttg acagcgtccg ccagatcgcc
      241 aggtccggcc tgcaggctca ggtctgcggc ctctcccgca tagtaaaaaa ggatgcccag
      301 gcctgccttg acgctgatgt ggacctggtc catgtctttt ctcccacctc cgatattcag
      361 atcgctcata ccatcaagat gagccatgat gagatcgtag agggagcaat gaggacggtg
      421 gagttcgtga aggataacgg acggacctgc ctcttctcgg ccatggatgc cacccgcaca
      481 tcgcccgagt tcctgcatcg catattcaaa gcggtggagg aagtgcactg cgatatcata
      541 aatataccgg atacggtcgg cgtggcagtc ccctcctcat tctaccggct ggtcaaggac
      601 atctgcgacg atgtccatat ggtggtggat gtccactgcc acaacgactt cggcctggcg
      661 gtggccaaca gcctggctgc agtggaagca ggcgcaaggg aggtccaggt ctgcgttaac
      721 ggcctggggg agagggcggg caacgccaac ctggcggagg tggtgatggg cctgcactcc
      781 atctacaatg ccaagaccag cattaagacc gagtacctgg tggagaccgc caggatggtc
      841 gagcggctca caaaggtgaa gatacccata actgctccta ttgtgggtga gaacgccttc
      901 tcccatgagt ccggaattca cagccacggg attatcgaga acagcaagac attcgagcct
      961 ggcataatga ccccggagat ggtgggccat aaaaggagga tagtcctggg aaagcatacc
     1021 ggaaagcatg ccgtggccaa ggtcctggag gaggcgggtt accggccgtc tgatgagcag
     1081 cttcaggaga tcttagagag gatcaaggag ctgggcgaca agggcaagca ggtcgggaac
     1141 atcgatcttc agaccatagc cgatgtggtc ataggagatg tggccaaaga aagccaggcg
     1201 gtggtgctca aggaggtctc agtgatgacc gggaacatca tcactcccac cgctacggtt
     1261 aaggccctgg tcagaggaaa ggagaaggtc ttggccaata ccggggtggg cccggtggat
     1321 gctgcggtca atgccgtaca gggactattg gatagcgata catctataca cctctgcgac
     1381 ttccggatcg aggccatatc cggcggtgcc gatgctctgg ctgaggtggt gatcggggtg
     1441 gaggatgacc ggggcaggag agtcagcgcc cgctcagcgc gagaggatat cgtcatggcc
     1501 tcggtggagg ccctggtaag cgccatcaac agattgatgc tgctggagga ggccgctgct
     1561 cgctaaatca atgatagggg atgtgcagag atatatgcag ggaactaccc aaggaaatat
     1621 gcagggaaac gtacagatga cattgagtgg ggtaatcgat tcgcattgcc atctggattt
     1681 caagcatttc aataaagatc gggaagctgt gatggagaac gcccggaagg cgggagtggt
     1741 caggatgatc aactccggcg tggactactc caccaacagc aagtcgctgg agctggcgaa
     1801 gaattacgac ttcatctctg ccaccctggg cctcagcccg aactcaattg acgggaagaa
     1861 cgatccggaa atcaagatga tcctggatca gatcgagagc aacgcccgcc aggcagtggg
     1921 aataggcgaa gcaggcctgg attactacca cactaaagat gctcccagca gagaacggca
     1981 ggcagagatc ttcaaaaagg tcatagcaat agccgagtcc ctgaacctgc ccttggtcat
     2041 ccattcccgg gatgcagaac agcctgctct ggacatggtc aagcacctgg ataaagtcgt
     2101 tttccactgc tacagcggca cccttcccac catgaaagag gcccaggata ggggatacta
     2161 catatctttg gccacaaacg tttgccgatc cggacatcat cagatcctgg cccggaacgt
     2221 ctccctcgat catttgctgg tggagacgga cagccccttc ctctcccctc gcaagggcag
     2281 aaatgagccg gcaaatgtac tggattccgt ccggctaatc gccaggataa aggggctgga
     2341 gccgcaggag gtggcggcgc agactgccac gaataccaaa cgaatttata acatccgttg
     2401 aaatgaccta aaatgattga gacgtcctgc aataagagcc tctgaggcca agacctcctg
     2461 aggccgaggc gtccctattc agggaagaag actggaaatc aggtcatgac atcaagaaaa
     2521 gggtcaacat ggatcggatg actgtgcctt tcgagcatat aatgaagtat ctgggctcga
     2581 gaaaggaaaa gggaagaaag agaatcggcc ttctggtgga tgggcccaat atgctcagga
     2641 aggagttcca gatggacctg gaggagatca gggacatcct gaaggactat ggcgacatca
     2701 agatgggcaa ggtattcttg aatcagtatg catctgagaa gctggtcgaa gcggtggaga
     2761 atcaggggtt tgagccggtg atctgcacca gcgatgtgga tgtgaggatg gcggtggagg
     2821 gtgttgacat gatctacaat ccagtcatcg acaccctggc cctggtcacc cgcgatgcgg
     2881 acctgaagcc ggttttaatg aaggccatgg agcatggcaa agagaccatc atcttcgggg
     2941 cggagcccgg gttctctgtg gccctgagaa attcggccga ttatgtgata gtgctgcgcg
     3001 atggccagta tgttaccga
//
LOCUS       DAOSGP010000164         2973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_31465_length_2973_cov_17.889308, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000164 DAOSGP010000000
VERSION     DAOSGP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31465_length_2973_cov_17.889308"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..>1335
                     /locus_tag="PLV49_05340"
     CDS             136..>1335
                     /locus_tag="PLV49_05340"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOG98303.1"
                     /translation="MFTILTGAQFGDEGKGKVIDLLAENYDVIVRFQGGDNAGHTVVV
                     GGKTYKLHLVPSGAIFGRRLLIGPGVVLNPRVLWNEIQSLESEGIKVDLGIDPKTTII
                     MPYHIELDSLREKARTEKIGTTNRGIGFAYVDKVAREEVQMGDLTDPALLEAKLKEIA
                     PGKEKAILDMGGDPTVVRYAPYIDEYLEIGKKLKSRIADVSREINIAILEEKSVLAEG
                     AQGAFLDVIHGTQKFVTSSFTIAGSACANLGVGPVMVDNVVGVIKAYITRVGEGPMPT
                     ELVDSLGEQMREKGGEYGTTTGRARRCGWFDAVLGLKSVYLNSYTEMALTKLDVLTGI
                     NPIKICVGYDLDGDTLGYPPESTTDLARCKPIYEEVPGWTEDITEVKDYDDLPENARD
                     YVEKLEDL"
     assembly_gap    1336..1435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1436..2814)
                     /locus_tag="PLV49_05345"
     CDS             complement(<1436..2814)
                     /locus_tag="PLV49_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG98304.1"
                     /translation="MIDSVKNREIAGILYQMAELLELHAENRFKIIAYSRAARAIESL
                     TEDIEQVCRDGRLEGIPGVGKAIAEKIKEYLRTGRIQSHQDLLADTPQGLAELLQISG
                     LGPKTVFMLHEKLDITNLDELEKAAREHRIRRLPRMGVVREKNILKSIERYRKRSNRI
                     LYSTAESIVDEILTYLGGIEGLEHATAAGSYRRRKETVGDIDILATAARPEEIVAAFV
                     KMPLVEEVLAKGPTKASVIMNDTIQVDLRIVEHRSYGTVLQYFTGSKEHNVSMRQLAL
                     DRGYSLSEYSLTRLANGQDLFFDQEEEVYQALGLQYIPPELREDRGEIEAALGGRLPR
                     LVEAKDIRGDLHVHSIWSDGRASIIELAQAARSLGYEYIALSDHSPSVGIAGGIGRKK
                     MEEKIEAVAEANDSLEGITVLMGAEVDIKADGSLDYPDDLLERMDVVVASVHMAQQQK
                     ERTITGRLIS"
BASE COUNT          648 a          799 c          771 g          655 t
ORIGIN      
        1 ggcaaaggga tctatgaggc tctgggaata gatgtcacag aagcgatagc acagcatgtc
       61 tgctccaatc tggatcacag aaagggatga tttccatcaa aatagttatc tacgtggccc
      121 gccatctcac ggagtatgtt tacaatactc accggtgcgc agtttggtga tgagggaaaa
      181 ggcaaggtta tagatctact ggcagagaac tacgatgtca ttgttcgctt ccagggaggc
      241 gacaatgcag gtcatacggt ggttgtggga ggaaagacat acaagctcca tctggttccc
      301 agcggcgcga tcttcggccg caggcttctg atcgggcccg gtgtggtgct taacccccgg
      361 gttttatgga atgagattca gtccctggag tctgaaggca tcaaggtcga cttgggaatt
      421 gatcccaaga cgacgatcat catgccctat cacatcgagc tggacagcct gcgtgagaag
      481 gcgaggacgg aaaagatcgg gaccacaaac cgcggcattg gatttgccta tgtggacaag
      541 gtcgctcgcg aggaggtcca gatgggtgac ctcaccgatc ccgccttact ggaagccaag
      601 ctgaaggaga tcgcgcctgg caaggagaaa gcaatccttg atatgggcgg agacccgact
      661 gtagtcagat atgctcctta catcgatgag tatctggaga tcggaaagaa actgaagagc
      721 aggatcgccg atgtctcgcg ggagatcaac atcgctatcc tggaggagaa gagcgtcctg
      781 gctgaagggg ctcaaggggc atttctggat gtcattcatg gcacccagaa gtttgtaacc
      841 tccagcttca ccatcgctgg aagcgcctgc gcaaatctgg gcgtgggccc ggtgatggtc
      901 gacaacgtgg tgggagtgat caaggcctac atcacccggg tgggcgaggg gcccatgccc
      961 acggagctag tcgattccct gggcgagcag atgagagaga agggtggaga atatggcacc
     1021 accacgggaa gggcgcgcag atgcggctgg tttgatgccg tcttaggcct gaagtccgtc
     1081 tatctcaaca gctacactga gatggccctc accaagcttg atgtgctcac gggcatcaat
     1141 cccataaaga tctgcgtggg ctacgatctg gacggcgata cccttggcta tccgccggag
     1201 agcaccacag accttgcccg ctgcaagccc atctatgaag aagtgcccgg ctggacggag
     1261 gatataaccg aggtcaaaga ctatgacgat ctgcccgaga atgcccgcga ttatgtagaa
     1321 aagctcgagg acctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagat
     1441 cagccttccg gttatcgtcc tctccttctg ctgctgggcc atatggaccg aggcaacaac
     1501 gacatccatt ctttccagaa gatcatcggg atagtccaaa ctgccgtctg cctttatgtc
     1561 cacctccgca cccatcagga cggtgatccc ttccaggcta tcatttgcct ctgccaccgc
     1621 ctcgatcttc tcctccatct tctttcgtcc gatccctcca gcgatgccta ccgatgggga
     1681 atggtcggat aatgcgatat actcatagcc cagagaccgg gcggcttggg ccagctcgat
     1741 tatggaggct ctgccgtccg accagatgct atggacatgc agatctcccc gaatatcctt
     1801 cgcctcgacg agcctgggca gcctccctcc cagcgccgcc tcgatctctc cccggtcctc
     1861 tcgcagctca ggcggtatgt actgcaggcc cagggcctgg taaacctctt cctcctgatc
     1921 gaagaacaga tcctggccat tggccaagcg ggtgagggag tactcgctca atgagtagcc
     1981 gcgatcgaga gcaagctggc gcatgctgac attatgctcc ttggagccgg tgaagtactg
     2041 aagcacggtg ccataggacc ggtgctcgac tatcctcagg tccacctgga tggtatcatt
     2101 cataatgacg ctcgctttgg tgggaccctt ggccaggacc tcctctacta aaggcatctt
     2161 cacaaaagcc gccacgatct cctccggcct tgcagcagtg gccagaatat cgatgtctcc
     2221 aacggtttcc tttcgtcggc ggtagcttcc tgcggcagtg gcatgctcca ggccctctat
     2281 acctcccaga tatgtcagaa tctcatcaac aatgctctca gcagtgctgt agagtatacg
     2341 gttgctgcgc ttcctgtacc tttcgatgga tttcaatata ttcttctcac gcaccacgcc
     2401 cattctggga agccggcgaa tacggtgctc cctcgcagct ttctccagct catccaggtt
     2461 ggttatatcc agcttctcat ggagcataaa gaccgtcttt gggcctaaac ccgagatctg
     2521 gagaagctcg gccaggccct gcggtgtatc cgccaggagg tcttgatgag actgaatcct
     2581 gccggtgcgc aggtactctt tgatcttctc agcgatcgct ttcccaacgc caggaatgcc
     2641 ctccagcctt ccatccctgc agacctgctc gatatcctct gtcagggatt cgatagccct
     2701 tgcagctctg ctgtaagcta ttatcttgaa gcgattctct gcatgcagct ccaggagctc
     2761 tgccatttgg tagagaatgc cagcaatctc gcggttcttg acggaatcga tcacaaaaac
     2821 ggattgatca cttgtggtat aagaataatg ctttctaaag aagctcttct atatgcttca
     2881 attcagaaat atctgtcaga tcgctgatcg actcatgcat cacatctatc tggcattcct
     2941 ttgctgcagc catgagattg gtccctccga cca
//
LOCUS       DAOSGP010000165         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_31534_length_2969_cov_20.498284, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000165 DAOSGP010000000
VERSION     DAOSGP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31534_length_2969_cov_20.498284"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLV49_05350"
     CDS             complement(<1..238)
                     /locus_tag="PLV49_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98305.1"
                     /translation="MKDFMGSRTTYYSLAKDRLKGKHPRIYNLILILTLMSLTGTAAF
                     LISDIFSFGIDYDNMSIQGGKGIIFPALWGDKKAS"
     gene            460..825
                     /locus_tag="PLV49_05355"
     CDS             460..825
                     /locus_tag="PLV49_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98306.1"
                     /translation="MKKIICKNCGVVNEISDGALDDGDDWLECTLPEGFEWILPAGKI
                     TPIVGEPLYISGFGEHLSRQAYRDKYDIDPEIAYQLMRGSSRVRSAAMAYAGKQRVAA
                     TVSSKRRSESWLDEDDWTS"
     gene            1282..1845
                     /locus_tag="PLV49_05360"
     CDS             1282..1845
                     /locus_tag="PLV49_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG98307.1"
                     /translation="MKETLEEIGTRLRELRELNRVSPEEMAEHLNVAVDTYNCYEDGM
                     LDIPASILIGIARRLNVDTGLLLTGEESKMSIFAITRKGEGVEVERRKQYHYQSLARK
                     FANKKAEPFIVTIDPKKESQSHPVHSLYSHPGQEFEYVLEGALKITINENEVILNEGD
                     SIFFDSTYDHVMEALSDRPVKLLAVVM"
     gene            1867..>2969
                     /locus_tag="PLV49_05365"
     CDS             1867..>2969
                     /locus_tag="PLV49_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG98308.1"
                     /translation="MTSLLPRYVSRLDFKSYDDFRENLKILVPENFNFAYDVVDVYAA
                     ECPDKCALVWCNDHGEAKTISFKELKKLSDKAANLFQGFGIKKGDTAMLTLKSRWEFW
                     VCMVGLNKLGAISIPSTHMLKAKDFLYRIKKADLKLIVCIGENGVPAEFDAAHREVGD
                     VPLVKALVGSRDLNREGWINFDLELERASETFVRPKGDQATKNDDILLAYFTSGTTGY
                     PKLVKHDQTYPLGHILTAKFWQNVEDDGLHYTVADTGWAKCAWGKIFGQWIAGSAVFV
                     YDYDRFDAGRMMDEMGKYGVTTFCAPPTIFRFMIKSDMSRYDFSRLKYAVTAGEPLNP
                     SVYESFLETTGLRLMEGYGQTETVVCISNYPWM"
BASE COUNT          798 a          713 c          740 g          718 t
ORIGIN      
        1 tgcttgcttt tttgtcgccc cagagagctg ggaatatgat gcccttgccg ccctgaatgc
       61 tcatattatc gtaatcaatg ccgaaagaaa agatatccga aatcagaaaa gcggctgtgc
      121 cagtcagtga catcagtgtg agtatcaaaa taaggttgta tattctggga tgctttccct
      181 tcaatctatc tttagccaga ctgtaatagg tggttctcga tcccataaag tccttcatac
      241 ttaatcagat attttctaaa gtttataact cttgcgattg aatattccat ttatttacga
      301 agtctctcaa ttgagatctc attttacttg attactaatg agtataaata atttatgttc
      361 gccattgcac tggatagagc ggctcattcc aataagtatt ttcttaggta aattatatat
      421 actgttatta accgaatact ctcattgaag gtgaggaata tgaaaaagat catctgcaaa
      481 aactgcggag tggtaaacga gatctccgat ggagctttag acgatggcga tgattggctg
      541 gaatgcaccc tccctgaggg ctttgaatgg atcctgcctg cggggaagat aactcccata
      601 gtaggcgagc ccctctacat ctccggcttt ggcgagcacc tctcccgcca agcctaccgg
      661 gacaaatacg acatcgatcc ggagatagcc tatcagctca tgcgcgggag cagtcgcgtt
      721 cgatccgcag ccatggctta tgccggcaaa cagagggtcg cggcaacggt cagttcaaag
      781 agaagatccg agagctggct tgatgaagac gactggacct cttgaaaaga tgagatacag
      841 ggcctacaac aggtcgaata tatggccatc tgcagctgcc aggagcctcc tggcaatcgc
      901 tcacgacagc tataataatt aaaaatcgct cataatggtc ataatggccc atagtattcg
      961 tttttgcggg gcaaaacacc ttttctctgg caaagaatcg cctggtcagt caggtttccg
     1021 ttttatctcc aagcttccct cagtcatcaa ttttagggtg aatgattaag tgattattat
     1081 gtatttcagg cacaattgag cataattgat aattaaggca ccatatcatc cctatccatc
     1141 atccgatatg gatcggcccg aattgagccc atttccagag atagattcaa atataaatgt
     1201 atggcagtct caaccctgca ttacctgtgg cctggatcta ctgctctgga ccatgaagtt
     1261 gggatgcgcc gaggaatgtg catgaaagag acgcttgagg agataggaac gcgacttcgt
     1321 gagctgagag agctgaaccg agtatcgcct gaagagatgg cagagcacct gaatgtggca
     1381 gttgacacgt acaactgcta tgaggacggt atgctggaca tcccggccag cattctcata
     1441 ggcattgcgc gacggctgaa cgtggataca ggcctgctcc taaccggcga ggagtccaag
     1501 atgagcatct tcgccataac ccgcaagggc gagggtgtag aggtggagcg cagaaagcag
     1561 tatcattatc aaagcctggc ccgaaagttc gccaataaga aggctgagcc ttttatcgtc
     1621 accatcgatc ccaagaaaga gagccaatca catccggtcc actcgctata cagccaccca
     1681 ggccaggagt tcgagtatgt tcttgagggc gcactaaaga tcaccatcaa tgagaacgag
     1741 gtcattctca atgaagggga ctcgatattc tttgattcaa catatgacca tgtcatggaa
     1801 gctttaagtg ataggccagt gaagcttttg gccgtggtca tgtaaacccg ttacagagga
     1861 ttaaaaatga catctttatt gcctaggtat gtctcgcgat tggatttcaa atcatacgat
     1921 gacttcagag agaacctgaa gatcctcgtc ccagagaact tcaatttcgc ttatgacgtc
     1981 gtcgacgttt atgccgctga atgtcctgat aaatgtgcac tcgtctggtg taacgatcat
     2041 ggcgaggcga agaccatcag cttcaaagag ctgaagaaat taagcgacaa agccgccaac
     2101 ctctttcagg gctttgggat aaagaaaggc gatacagcaa tgctcaccct caagagccgc
     2161 tgggagttct gggtgtgcat ggtgggcttg aacaagctcg gcgcaatctc catcccttcc
     2221 acccatatgc tgaaagccaa agactttctc tatcgcatca aaaaagcaga tctgaagctg
     2281 atcgtctgca tcggtgagaa cggcgttcct gcagagttcg atgccgccca cagagaggtg
     2341 ggagacgttc ccctggtcaa agccctggtg ggaagccggg atctgaatcg agagggctgg
     2401 atcaacttcg atctggagct ggaacgggca agtgaaacct tcgttcggcc taaaggagat
     2461 caggcgacta aaaacgatga catactgctg gcatacttca cctccggcac caccggctat
     2521 ccgaagctgg tcaagcacga tcagacctat cccctgggac atatcctcac cgccaagttc
     2581 tggcagaatg tggaggatga tggattgcat tacacggttg ccgataccgg ctgggccaaa
     2641 tgcgcctggg gcaagatatt cggccaatgg atagcaggat cggccgtctt cgtctatgat
     2701 tacgaccgct tcgatgccgg aaggatgatg gacgagatgg gcaaatacgg cgtgaccacc
     2761 ttctgcgccc cgcccaccat cttcagattc atgatcaaga gcgatatgtc tcgctatgac
     2821 ttctcccgcc tcaagtatgc agtcacagca ggtgagcccc tcaatccttc ggtatatgag
     2881 agcttccttg agaccaccgg cctgcggctg atggagggat acggccagac ggagaccgtg
     2941 gtctgcatct ccaactatcc ctggatgga
//
LOCUS       DAOSGP010000166         2967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_31572_length_2967_cov_13.477679, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000166 DAOSGP010000000
VERSION     DAOSGP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31572_length_2967_cov_13.477679"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLV49_05370"
     CDS             <1..834
                     /locus_tag="PLV49_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOG98309.1"
                     /translation="SGNSIGDGNLFRPLRAHWKPLWFYFFDKSTGEAVYLEANSEALS
                     KSLDEFLDLPDDQVFSKISKANVDIEYLQNHTDEGNVTFNDKAFNGNEFGLVAMSNVW
                     ARGASYDFLQATAFHDHLCPGVTSGLHIAEFVEEKLPITNSSESYKVISCPNWCKEDL
                     FQMRWDATPGKSGMFVMALTDAEKKAVPNVAGIYIRWNDTAKEGDALVLAYNFSAVDL
                     PKWTGPSWGSKIYQDIVLMPYENNPEAFITVLKEFKVDAATLSQMQNAGMHPLKVAGV
                     M"
     gene            1036..2718
                     /locus_tag="PLV49_05375"
     CDS             1036..2718
                     /locus_tag="PLV49_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOG98310.1"
                     /translation="MPDNNNNDSVAGAAACKSSHWLHRYRSFLVDPGTLITLSCGILL
                     ILAVISDPGGLYHGEVHEGGSILYLAAALLGSSYIWWSALQGIRERDFTADIPVSIAT
                     MAAITIGEYAAAAVVAVLLLLGGLLEEFVAARANQSLESLSCLLPDSVTVRREGRDLT
                     VCLEEVRAGDMLLVRSGERIAVDGEVVSGFASVSQAAITGESLAVEKKAGDKVFAGTL
                     LEVGAIEVLVTGIGSETTLGQIRTLIEEAQAHKPPIERLLNRYAKIYMPTALIAGALL
                     WWWSGDVMRAITMLIVFCPCVIVLATPTALVAAIGNAALRGSLIKKGATIEALSNVDT
                     VIFDKTGTLTAGKPKLLKIEALGDIPHDKLLKKAARGEKFSEHPLGRAMVDEAKSRDL
                     DLVDPQLFEPLPGLGIRASVDGEEIFLGRINSLEREGIFVGQDIKERAESLEATGCSV
                     ILLAAGGRTEGLFVFEDELRPESHNVVQKLDELGLDVVIVTGDNRASTERVASRIGVR
                     ELFFEKMPQEKVQIVKELQSKGRKVAFVGEGGERWPGSGPGGCRHSHGHCGN"
     gene            2654..>2967
                     /locus_tag="PLV49_05380"
     CDS             2654..>2967
                     /locus_tag="PLV49_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOG98311.1"
                     /translation="MNDGPALAQADVGIAMGIAGTDVAMETADIGLLSDDLSKMPHLI
                     KVSRKAIKTIKHNLAFSLGVLAVAVALTVPGILTPVSGALLHELSSIPVILNSMRLIA
                     YGP"
BASE COUNT          703 a          739 c          852 g          673 t
ORIGIN      
        1 agcggaaaca gcatcggcga tggaaacctt ttcaggcctc tgcgcgctca ctggaagccc
       61 ctctggttct acttcttcga taaatccacc ggcgaagcag tgtatctgga ggcaaactcc
      121 gaggccttga gcaagagtct cgatgagttt cttgacttgc cggacgatca ggtattcagc
      181 aagatatcaa aagccaacgt ggatatagaa tatctgcaga accatacaga tgaaggcaat
      241 gtcaccttca acgataaagc cttcaacggc aatgagttcg gacttgtggc aatgtccaat
      301 gtctgggcaa ggggagcatc ctatgacttc ctccaggcta ctgcattcca tgaccatctc
      361 tgccctgggg tcaccagtgg ccttcatatc gctgagtttg tcgaggagaa gctgccgata
      421 accaatagca gcgagagcta taaggtcatc tcatgcccca actggtgcaa agaggaccta
      481 ttccagatgc gctgggatgc cactcccgga aagagcggaa tgttcgtgat ggccctgacg
      541 gatgccgaga agaaggcagt gcccaacgtc gccggcatct acatccgctg gaatgatact
      601 gccaaagagg gtgatgcact ggtgctggcc tacaacttca gcgctgtaga tctgccaaag
      661 tggacaggcc cctcctgggg atcgaagata taccaggaca tcgtcctcat gccatatgaa
      721 aataatccag aggcgttcat aaccgtcctc aaggaattca aggtggatgc tgccaccctc
      781 tcccagatgc aaaacgccgg catgcatccg ctgaaggttg cgggcgtaat gtaatttgca
      841 taatgaaaga aggctgctgg atcaagtagc caattctttc actctaatga gcaatgagca
      901 gcttatctat taactttttt ttccatatgt ctcgataact atttatctag ccttctcctg
      961 cttgattgtt actgcagtca atatatgtaa cataaaagct tactggaatc agacgaatta
     1021 ggagccttag ataagatgcc tgacaataat aacaatgatt cggtcgccgg agccgctgcc
     1081 tgcaagagca gtcattggct gcatcgctac aggagctttc ttgtcgatcc cggcactctc
     1141 atcaccttat cctgcggcat tctcctgatt ctggctgtca tcagcgatcc gggcggcctg
     1201 tatcatggtg aggtgcatga gggaggaagc atcctctatc tggccgcggc ccttctcggc
     1261 tcctcttata tttggtggtc tgctttgcag ggcataaggg aacgggactt cacagccgat
     1321 attcccgtat ccattgccac aatggcggcg atcaccattg gcgagtatgc agcagctgcg
     1381 gtggtggccg tccttctcct cttaggaggc ctcctcgaag aattcgtcgc cgctagggct
     1441 aatcaatcgc ttgagtccct atcctgtctc ctgcccgata gcgttaccgt gcgcagagag
     1501 ggccgagatc tgacagtgtg cctggaggag gttcgagcag gggatatgct tcttgtccgg
     1561 tccggagaga ggattgctgt ggatggcgag gttgtctctg gctttgcctc tgtcagccag
     1621 gcagccataa ccggggagag cctggcggtt gagaagaagg caggggataa ggtctttgcc
     1681 ggaacccttc tggaggtggg tgccattgag gtgctcgtca ccggcatagg gagcgagacc
     1741 actcttggcc agatccgcac tctgatcgag gaggctcaag cccataagcc gcccatagag
     1801 aggctactaa accgctacgc taagatctat atgcccacag ccctcatcgc aggagcgctt
     1861 ttgtggtggt ggagcgggga tgtcatgagg gccatcacca tgctcatcgt cttctgcccc
     1921 tgcgtgattg tgctggccac gccgacggct ctcgttgctg ccattggaaa tgccgccctg
     1981 agagggagcc tcatcaagaa aggggcaacc atcgaggctc tatcaaatgt ggatacagtg
     2041 atattcgaca aaaccggaac gctgacggca ggaaagccga agcttctcaa gatcgaggct
     2101 ctgggagata ttccccatga taagctgcta aagaaggctg ccagggggga gaagttcagc
     2161 gagcatcctc tgggcagagc gatggtcgat gaggctaaaa gcagggatct ggatcttgtc
     2221 gatccccaac tctttgaacc tctgccggga ttgggaataa gggctagtgt tgacggtgag
     2281 gagatatttc tgggaaggat aaacagcctg gaaagggagg gtatttttgt cggccaggat
     2341 attaaagaga gggctgaatc tctggaggct actggctgca gcgttatact cctggccgca
     2401 ggcggtcgga cggaggggct attcgtattc gaggacgaac tgagaccaga gtcgcataat
     2461 gtcgtgcaga agctggatga gctgggtcta gatgtggtga tcgtaaccgg tgacaatcga
     2521 gcgtctacag agcgggtggc cagcagaatt ggagtcaggg agctattttt cgagaagatg
     2581 ccgcaggaga aggtccagat agtaaaggag ctgcaatcca agggacgcaa agtggcattt
     2641 gttggcgagg ggggtgaacg atggcccggc tctggcccag gcggatgtag gcatagccat
     2701 gggcattgcg ggaactgatg tagccatgga gacggcggat ataggccttc tctctgatga
     2761 cctctccaag atgcctcatc taatcaaagt ctcacgcaag gcgatcaaga ccataaagca
     2821 caacctggcc ttctcccttg gggtgctggc ggtagcagtt gcactgactg tgccggggat
     2881 actgacaccc gtctccggag cactactcca tgagctgtca tccatcccgg tgatattgaa
     2941 ttccatgagg ctcattgcat acgggcc
//
LOCUS       DAOSGP010000167         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_31770_length_2955_cov_16.790345, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000167 DAOSGP010000000
VERSION     DAOSGP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31770_length_2955_cov_16.790345"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1274)
                     /locus_tag="PLV49_05385"
     CDS             complement(<1..>1274)
                     /locus_tag="PLV49_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOG98312.1"
                     /translation="YGSCRLCTVEIEKNGKKRFVTACNYPVEDGLVVRTATPAVLDIR
                     RMIAELLLSRCPKEKQIQNLASEYGVIEPRFKLGEEKCILCGLCCRVCGEMVGVFAIN
                     FQNRGTDRSVGAPYGELSEDCIACGACSLVCPTSAISAQRNIFPLTAEDIIRIEEEHL
                     SGERDADLGVCSELFAARTEIQGQDGGVVTSLLARCLDKGVIDAAVVVYQKENNGGRA
                     AAVDDIEGVIKAKGTKYVRVSALAPLIDALRGGKRRVAVVGTPCQIRVIRKLEQLDYF
                     KDEFPDAEIFLVGLFCFESFDYRRLRDHAKGLLGIDIEDADKIQIAKGRYIATLDGME
                     HSCSVRELENDIREGCRFCGDLVSRLADISIGSVGSAEGYSSVIVRSEKGKKLLDWLS
                     FCREKAVREDIVKLARMKRRNADRNLERIRKG"
     assembly_gap    1275..1374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1375..2608)
                     /locus_tag="PLV49_05390"
     CDS             complement(<1375..2608)
                     /locus_tag="PLV49_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG98313.1"
                     /translation="MTPEEVIKEVKISNLRGRGGGGFPTGRKWESTREATGDPKYVIV
                     NCDEGDPGAFMDRALMEGNPHSVLEGLIIAAYAMGSHKGFIYVRMEYPLAVQNAALAL
                     QQAREYGLLGDNILGTGFDFDVSIHRGAGSFVSGESTALMNALEGWVGEPRPKYVHTS
                     QKGLWGRPSALNNVETFANVPLIIKHGAKWFNSIGTERSKGTKIFSLVGKVNNTGLVE
                     VPMGMTLADIIYKIGGGIPGNKKLKGVQTGGPSGGIIPDRYLNTPVDFDELTSLGSMM
                     GSGSIIVIDEDTCAVDLARYFVDFLCSESCGKCLPCREGLKRMREILDSIESGRGTET
                     DIQTLQEIAELMRTASLCALGRTAVNPVLSTIRYFREEYDAHIKDKRCPALSCKSLVS
                     YSIDPEICIGCDLCRENCP"
     gene            2711..>2955
                     /locus_tag="PLV49_05395"
     CDS             2711..>2955
                     /locus_tag="PLV49_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98314.1"
                     /translation="MVFIKGNFALNDGILFRFRILVEKAVNQVAFEYRLLHDIRDIIL
                     MNANIIDASGVDHHQSSFFAKARATGLFDLYLLFQAL"
BASE COUNT          628 a          869 c          634 g          724 t
ORIGIN      
        1 tccttttctg atcctctcaa gattgcggtc tgcatttcgc ctcttcatcc tggccagctt
       61 cacgatgtcc tctcgaactg ccttttctct gcaaaacgat agccaatcca gcaacttctt
      121 ccccttttcc gatctgacga tcactgagga gtatccttcc gcgcttccca ctgagcctat
      181 ggagatatcg gccagccttg agaccagatc gccgcaaaac cggcatcctt cacggatgtc
      241 attttccagc tcccgcacgc tgcaggagtg ctccattcca tccagagtcg caatgtatct
      301 cccttttgcg atctgaattt tatccgcatc ctctatatct atgcccaaaa gccctttagc
      361 atgatctctc agtcttctgt aatcgaagct ctcgaaacag aacaggccaa cgagaaagat
      421 ctctgcatct ggaaactcat ccttgaagta atccaattgc tccaattttc tgatcactct
      481 gatctggcag ggagtgccaa ccacagcaac ccttctcttt cctcctctca aagcatcaat
      541 cagcggcgcc agagcggata cgcgcacata ttttgtcccc ttggccttta tcactccttc
      601 tatatcgtct accgctgccg ctcttcctcc attattctcc ttttgataaa ccaccacagc
      661 agcatcaatg acgcccttat ccaggcatcg agcaagaagg gaggtgacaa cccctccatc
      721 ctgtccctgg atctctgttc tggcggcgaa aagctcgctg cacactccca gatcagcatc
      781 cctttcgccg gacagatgct cctcttcgat cctgatgata tcttctgcag tgagcgggaa
      841 gatgtttctc tgagcggata tggcactggt ggggcatacc agagagcagg caccacaggc
      901 tatgcagtcc tccgagagct ctccatacgg cgcgcccaca ctccgatctg ttcccctgtt
      961 ttggaaattg atggcgaata cacccaccat ctcaccacat acccggcaac aaagcccgca
     1021 gagaatgcat ttctcctccc ccagcttaaa tcgaggctca atgactccat actcactggc
     1081 caggttctgg atctgcttct ctttcgggca tctggacaat aagagctcag cgatcatccg
     1141 acggatgtcc aggaccgcag gggtggcagt cctgaccacc agaccgtctt ctaccggata
     1201 gttgcaggcg gtgacaaacc tcttctttcc attcttttca atctccaccg tacagagcct
     1261 gcaagaccca taggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaggac
     1381 agttctcccg gcacaggtcg cagcctatgc agatctcagg atcgatggaa tatgacacaa
     1441 gagacttgca ggatagggcg ggacatctct tatctttgat atgggcatca tactcctctc
     1501 ggaaataacg aatagtgcta aggaccggat tgactgccgt tcgtcccagg gcgcagagcg
     1561 atgccgttct catcagttct gcaatctcct gcagggtctg gatatccgtc tccgtccccc
     1621 ggccggactc gattgaatca agaatctctc gcatccgttt cagcccctcc cggcagggca
     1681 ggcatttgcc gcaggactcg ctgcagagga agtccacaaa gtaacgggcc aggtcgacgg
     1741 cacaggtatc ctcatcgatg acgataatgc tccccgaacc catcattgag cccagactgg
     1801 tcaattcatc aaagtccacc ggtgtattca gataccggtc cggaattatc ccaccagacg
     1861 gccctccggt ctgcacccct ttgagcttct tgttcccggg aattccccct ccaatcttgt
     1921 atattatatc ggccagggtc atgcccatag ggacctccac cagaccggtg ttattgacct
     1981 tgcccaccag agagaagatc ttggtccctt tgctcctctc ggttccaatg gagttgaacc
     2041 attttgcgcc atgctttatg atcaaaggca cattggcaaa ggtctcaacg ttgttcaggg
     2101 cgctcggccg gccccagagg cctttttggg aggtgtgaac atacttgggc ctgggctcgc
     2161 ccacccatcc ctccagggcg ttcatcagag cggtcgactc tccggagaca aaggagccgg
     2221 cacccctatg aatgctcaca tcaaaatcaa agccagtgcc cagaatatta tcacccaaga
     2281 gaccgtactc ccgagcctgt tggagggcga gtgcggcatt ttggaccgct aatgggtatt
     2341 ccatgcgcac atagatgaag cccttatgcg atcccatggc gtaggcggcg atgatcagcc
     2401 cctccaggac gctatgagga ttgccctcca tgagagcccg gtccataaat gcacctggat
     2461 cgccttcatc gcagttgacg atcacatatt ttggatctcc tgtggcctct cgggtcgatt
     2521 cccattttcg tccggtggga aatccgcctc cacctctgcc ccggaggttt gagatcttca
     2581 cctccttgat cacctcttct ggggtcatct cggatagagc tctggccagg gcactgtatc
     2641 cgcccagcgc tatataatct tctatatttc taggatctat ttttatgtta ttttttataa
     2701 gatgcctcat ttggtattta taaaagggaa tttcgctctc aacgacggca ttctcttccg
     2761 tttccggatc cttgtagaga aggcggtcaa ccaggttgcc tttgagtatc gtctcctcca
     2821 cgatatcagg gacatcatcc tgatgaacgc caatataata gacgcctctg gggtagacca
     2881 ccaccagagt tcctttttcg caaaagccag ggcaaccggt ctctttgatc tctatctcct
     2941 tttccaggcc ctttt
//
LOCUS       DAOSGP010000168         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_32068_length_2935_cov_19.095833, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000168 DAOSGP010000000
VERSION     DAOSGP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32068_length_2935_cov_19.095833"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..911
                     /locus_tag="PLV49_05400"
     CDS             36..911
                     /locus_tag="PLV49_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOG98315.1"
                     /translation="MAAGCTKCNMTWQKLLVLGFLAGAYIAFGALLSEIVAGGLSNGT
                     ITGPDGEIWKIALPGGLVKFAAGAVFPVGLMLVVIAGSELFTGNCMFAPISVLNGEAS
                     LGGLVKNWSLVYVGNLVGSVFVAYFLAYQCGLFDNLPWAGWAATVANTKCGLDFGTAF
                     LRGIGCNWLVCLAVWLAISSEDVISKIFSCWFPIMAFVTIGFEHSVANMFFIPLGIFA
                     ANDPAIAANLATAGVATTNLAGGIGWYNFFITNLIPVTLGNIVGAALFVAALYWYVYL
                     KGPVCKVIPEKGAKK"
     gene            946..>1145
                     /locus_tag="PLV49_05405"
     CDS             946..>1145
                     /locus_tag="PLV49_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG98316.1"
                     /translation="MNQNNGERNGYLVIIGCCIAIFWPGALTFGFPGVMASLWQEMFH
                     VGSGATGSTIFFMLSAVGIFMFL"
     assembly_gap    1146..1245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1246..2034
                     /locus_tag="PLV49_05410"
     CDS             <1246..2034
                     /locus_tag="PLV49_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG98317.1"
                     /translation="VFGLMLNAFGYRNMSLVLAVLTLTAGLLGAYFARERDDETIKRQ
                     NEMRDALKRQIREGNEIDKDEMRRSLTVSETLHTRSFWFLWTTWILAGAAGVSMTVLS
                     TGYGLSLGLPLEGAVLILTAFNLTNGTGRLASGYFSDRLGRRRVMSLAFLAAGLAYFL
                     FPLAGDLPTSAILAAVVGLSFGTLFSVSAPLVADCFGLDHFGAIFGLTFAAYGFLAGP
                     LGPTLSGYLLDLNGDNFVPIFLYLGAFSLLAALSIRQVVSPEWR"
     gene            complement(2093..2731)
                     /locus_tag="PLV49_05415"
     CDS             complement(2093..2731)
                     /locus_tag="PLV49_05415"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG98318.1"
                     /translation="MIRIISLDMDGTLVNSRFVDKVWMEGMPRLYAERTGLDLPAARE
                     YVIGEYMKIGSDHLEWYDLKFWIDKFGLSIGKEELLELYEDEIEVYPEVEEVLELLSE
                     NYELVVTSNAAREFIDIELDGLQGYFREVFSATSDFREVKKSPLLYGAICAHLDARPF
                     EVLHIGDHYSYDYESALDAGLDALFLDRRGERQGPEVIGDLREAVELIDGCA"
BASE COUNT          579 a          800 c          770 g          686 t
ORIGIN      
        1 cattcaaagt tcccgctgat atcgctaagg cagccatagc tgccggatgc acgaaatgca
       61 atatgacctg gcagaagctg ctggttttag gctttttggc cggggcctat atcgcctttg
      121 gagccctttt gagcgagatt gtggccggag gcctctccaa tggcaccata accggcccag
      181 acggcgagat ctggaagatc gcccttccag ggggcctggt taagttcgcc gccggagcgg
      241 tcttccccgt gggcctgatg ctggtcgtca tcgccggatc ggagctgttt accggcaact
      301 gcatgttcgc ccccatcagc gtcctgaacg gcgaggcaag cctgggaggc ctggtcaaga
      361 actggtcgct cgtctacgta ggcaacctgg tgggatcggt cttcgtcgcc tacttccttg
      421 cttaccagtg cggactgttc gataacctcc cctgggcggg ctgggcggca accgtggcca
      481 acaccaaatg cggcctggac ttcggcaccg ccttcctgcg agggataggc tgcaactggc
      541 tggtttgcct cgccgtctgg ctggccatca gctcagagga cgtgataagc aagatcttct
      601 cctgctggtt ccccatcatg gccttcgtta ccatcggctt tgagcatagc gtggccaata
      661 tgttcttcat ccctcttggc atcttcgcag ccaacgatcc ggccatcgcc gctaatttgg
      721 cgaccgctgg agtggctaca accaacctcg ccggtggaat aggatggtac aacttcttca
      781 tcaccaatct tattccagta acgctgggca atatcgtggg agcggccctc ttcgtggcgg
      841 cgctctactg gtatgtctat ctcaaggggc cagtctgcaa ggtaatcccg gagaagggag
      901 caaagaagta gcaatgcccg gcagatctat tggctgcaat agagcatgaa tcaaaataat
      961 ggagaaagga atggatatct ggttataatc ggctgctgca tcgctatctt ctggccgggc
     1021 gccctcacct tcggctttcc cggagtgatg gcctccctat ggcaggagat gtttcatgtt
     1081 ggcagcgggg ccactgggtc taccatcttc ttcatgctct ctgccgtggg catcttcatg
     1141 tttctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtatt cggccttatg
     1261 ctgaatgcat tcggctacag gaacatgagc ctggtcctgg cagtccttac tctgactgca
     1321 ggcctgctgg gcgcatattt cgcccgggag cgggatgatg agacgataaa aaggcaaaac
     1381 gaaatgaggg acgccttgaa gcggcaaatc agagagggga atgagataga taaggatgag
     1441 atgaggcgat ctctcactgt atctgagacc ctccatactc gaagcttctg gtttctctgg
     1501 acaacctgga tcctggccgg agcggcggga gtctccatga ccgtcctctc caccggctat
     1561 ggattatctt taggccttcc tctggagggt gcggttctca ttctcaccgc cttcaacctc
     1621 accaatggca ccgggaggct ggcgagcgga tacttctccg accggttggg aagaaggagg
     1681 gtcatgagcc tggccttcct tgctgctggt cttgcatatt ttctctttcc cctggcagga
     1741 gatctgccta catcggcaat cctggcagca gtggtgggac tctcttttgg caccttattc
     1801 tccgtctctg ctccattggt ggcggactgc ttcggcctgg accattttgg tgccatattc
     1861 ggactaacct ttgcagccta cggatttctc gccggaccac tggggcccac cctttctgga
     1921 tatctgctgg atctgaatgg cgacaatttc gttcccatct ttctctatct gggggcattc
     1981 agcctgctgg ccgctctctc catccggcag gtggtgtcgc cagagtggcg ttgaaaggca
     2041 tcaaaaaaag tcaagatgat ctagtcatga agagatgacc aaaggtcagc cctcatgcgc
     2101 acccatcgat cagctccact gcttccctca ggtcgcctat gacctcgggg ccctggcgct
     2161 cgccccttcg gtccaggaag agggcatcaa ggccagcatc caaagcggac tcatagtcat
     2221 agctgtagtg gtcgccgata tgcagaacct caaagggcct ggcatccaaa tgagcgcaga
     2281 tggctccata gaggagaggc gatttcttga cctccctgaa atcgctggtt gcagagaaga
     2341 cctcccggaa gtagccctgc aggccatcca gctcgatgtc gatgaactcc cgagcggcat
     2401 tggatgtcac caccagctca tagttctcgg aaaggagctc cagcacctcc tctacctcgg
     2461 gatatacctc gatctcgtcc tcgtagagct ctaaaagctc ctctttgcct atggagaggc
     2521 cgaacttgtc tatccagaac ttgaggtcgt accactccag gtgatcgctg ccgattttca
     2581 tatactcgcc aatcacgtat tctctggctg cgggaaggtc caatcccgtc ctctcggcat
     2641 aaagcctggg catgccttcc atccagacct tgtccacaaa cctcgaattt accagggtgc
     2701 catccatgtc cagggatatt atgcgaatca tcttgaactc ctgttgaaga ggaaatagtt
     2761 gcctattagg tttattattc ttttggatgg gatcatgaga tcgccttgcg gggtggcaag
     2821 acgaacatag cgctcagtca catctgcatt ggccggctca aaacgaagag ctggatgagg
     2881 cctggctcat ggaaccccca ccatccatca tgctgatggc tgtcttggag cttat
//
LOCUS       DAOSGP010000169         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_32213_length_2927_cov_17.272284, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000169 DAOSGP010000000
VERSION     DAOSGP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32213_length_2927_cov_17.272284"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLV49_05420"
     CDS             complement(<1..245)
                     /locus_tag="PLV49_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron cofactor
                     biosynthesis protein"
                     /protein_id="HOG98319.1"
                     /translation="MSEESYPVANVRGQEVPYDPEQLRRISEHPCFSEKACHSFGRMH
                     LAVAPKCNIQCKYCIRDFDCVNESRPGVTSQVLKPKEA"
     gene            364..678
                     /locus_tag="PLV49_05425"
     CDS             364..678
                     /locus_tag="PLV49_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0008312 - 7S RNA binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HOG98320.1"
                     /translation="MPDKSKIVIWPIYFDAARSRDEGRMVSREYAINEPTLDMIITAS
                     IKSGLKPEIEREKKHPKTWHKPEAAGRILVAKKGSKSATLKKIAGSLKMKYKKQATGK
                     RG"
     gene            688..1320
                     /locus_tag="PLV49_05430"
     CDS             688..1320
                     /locus_tag="PLV49_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HOG98321.1"
                     /translation="MIDLHTHTTFSDGELIPSELVRRAEVLGYTAIGITDHADYTNIE
                     HIICCVSKAKYLEGILDIRILPGVELTHVPPQKIAQLADLARNLGAEIVVVHGESPVE
                     PVRPGTNRAALEAGVDILAHPGFITLEEAEMARDNDICLEITSRSGHNITNGHVVQMA
                     IQARAKMVVDTDAHAPEDLIDSERAIQIAFGAGLTLEEAREIVLHHAIIR"
     gene            1389..>1724
                     /locus_tag="PLV49_05435"
     CDS             1389..>1724
                     /locus_tag="PLV49_05435"
                     /inference="COORDINATES: protein motif:HMM:NF021491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2209 family protein"
                     /protein_id="HOG98322.1"
                     /translation="MSGRHSVKGRYKMVCAVLSARVSPNFIEKVHSVRLVPRIAEALD
                     LNVIADLISDACLCLPGTIVAEQGDLYNLEVWRAQSILGRDFKYPETIAERTAIELAH
                     HISLAGRRLI"
     assembly_gap    1725..1824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1825..>2927
                     /gene="hflX"
                     /locus_tag="PLV49_05440"
     CDS             <1825..>2927
                     /gene="hflX"
                     /locus_tag="PLV49_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOG98323.1"
                     /translation="AGYRVLQEIKQRRERDHRFQIGRGKIEEALSCHPKKLIFYNPLS
                     PTQVFNIRSEFPVQVLDRFNLILEIFASRASTREAKLQVELARLSYDAPQVRSELALK
                     KRGEQPGFRGAGAYEQSMYHDIRGRMAKIRGELKEVEAMGEGRRKRRRELGFDLIALA
                     GYTNAGKSTLLNTLTGSVVNAQDQPFTTLSPTTRALEINGRRAMLTDTVGFIDDLPHF
                     LIKAFQSTLSEIAQADLVLLVADLSDPLELLRRKLVASHKALWDCQVTAPMITVLNKM
                     DRLDEFDARMRFDQIKDLAPNPVMVSAHSSQGQESLKECIYQHLPPLKEYQIRLPYTS
                     QSFGELSRLYGTAELLDVSYEDELVLSLRGRAE"
BASE COUNT          686 a          728 c          802 g          611 t
ORIGIN      
        1 gcctccttgg gctttaaaac ctggctggtg accccggggc gggactcgtt cacgcagtcg
       61 aagtcccgga tacagtattt gcactggata ttgcattttg gggccacggc cagatgcatc
      121 ctgccaaagc tgtgacaggc cttctcgctg aaacagggat gctctgatat cctcctcagt
      181 tgctccggat cgtaggggac ctcctgtcct ctcacattgg caacgggata actctcttca
      241 ctcatgatcg aactccacta ttgtattgct cttagtatcc tttcgcatag ctaaaagact
      301 tgcgtgccag aaggtgaagt tagacaaaga tactttaagg aggcgagaga aggcaggagg
      361 tatatgcctg acaagagcaa aattgtgatc tggccgatat atttcgatgc cgcccgttcc
      421 cgtgatgagg gcagaatggt ctccagagag tacgccataa atgagcccac cctggatatg
      481 atcataacgg catcgataaa atccgggctg aagccggaga tcgagaggga gaaaaagcac
      541 ccaaagacct ggcacaaacc agaggcagca ggccgaatat tggtggcgaa gaagggctcc
      601 aaatccgcta cattgaagaa gatcgcagga tccttaaaga tgaaatataa aaagcaggcc
      661 actggcaagc gaggttgagc ggtatatatg atagatctgc atacccatac aaccttcagt
      721 gatggcgaac tcatacccag cgagctggtg cgccgggcag aggtcttggg ctataccgcc
      781 attggcataa ccgatcatgc tgactatacc aatatagagc acatcatttg ctgcgtctcc
      841 aaggccaagt atctggaagg catactggat atcaggattc ttcccggggt ggagctgact
      901 catgtgccgc cacagaagat agcgcagcta gcggatctgg cccgaaacct gggcgcggag
      961 atcgtggttg tccacgggga atctcctgtg gagcccgtcc gtcctggcac caaccgggcg
     1021 gctctggagg caggtgtgga tatccttgcc catcccgggt tcatcacctt agaggaggcg
     1081 gagatggcca gggacaacga tatctgtctg gagataacct cacgctccgg ccataacata
     1141 accaacggcc atgtggtgca gatggcaatt caggccaggg cgaagatggt ggtggacact
     1201 gacgcccatg ctcccgaaga tctcatcgat agcgaaaggg caattcagat tgcatttggc
     1261 gcgggactga ctcttgaaga ggccagggag atcgtcctgc accatgccat tataagatga
     1321 gaccattatt gagtggattc tgcaggcatc taccgggggt tgaatagata gacatcgtag
     1381 cgattgacat atcgggccgg cattctgtaa agggcaggta taagatggta tgtgccgtcc
     1441 tatccgcccg ggtatcaccc aattttattg agaaggtcca ttcggtcagg ctcgtgcccc
     1501 gcatcgctga ggctctggac ctcaatgtta ttgctgacct gataagcgat gcctgcttat
     1561 gcctcccggg aactattgtg gctgagcagg gggatttata caacctggag gtctggaggg
     1621 cacagagcat cctaggaagg gatttcaagt atcctgagac gatagcagaa aggaccgcca
     1681 tagagttggc tcatcatata tctctggccg gtcgaaggct tatcnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnngccggc tatcgagtcc tgcaagagat aaagcagcgc
     1861 cgagagaggg accaccgttt tcaaatcggc aggggaaaga tagaggaggc cctatcctgc
     1921 catccaaaaa agctcatctt ctacaatccg ctcagtccca cccaggtatt caacattcgt
     1981 agcgagtttc cggtccaggt cttggaccgg ttcaatctga tcctcgagat ctttgcctcc
     2041 cgggcttcca ccagggaggc caagctgcag gtggagctgg cccgtctgag ctatgatgct
     2101 cctcaggtga gaagcgagct tgcccttaag aagcggggtg agcagccagg attccgcggt
     2161 gcaggtgctt atgagcagtc gatgtaccac gacatccggg gaaggatggc aaagatcagg
     2221 ggggagctta aagaggtgga ggcaatgggc gagggaagga gaaagagacg cagagaactg
     2281 ggctttgacc tcatcgccct ggccggatac accaatgctg gcaagagcac cctgctcaac
     2341 accctcacgg gctctgtggt caatgcccag gatcagcctt tcaccaccct ctctcccacc
     2401 accagggctc ttgagatcaa tggccgccgg gccatgctta ccgatacagt gggatttatc
     2461 gatgatctgc cccacttcct gatcaaagcc ttccaatcca ccctttccga gatcgcccag
     2521 gcggatctgg tccttttggt tgccgacctg agcgatcctt tggagcttct caggcgcaag
     2581 ctggtggcat ctcacaaggc cctctgggac tgccaggtga ctgcgcccat gatcaccgtt
     2641 ttgaacaaga tggaccgatt ggatgaattc gatgccagga tgaggtttga tcagataaag
     2701 gatttggctc ccaatccggt tatggtctcg gcacattcct ctcagggtca agagagcctc
     2761 aaggaatgca tctatcagca cctgccacca ttaaaggagt atcaaatccg gctcccgtat
     2821 acctcccaga gctttggaga gctgtcccgc ctctatggga cggctgagct tctggatgtg
     2881 agctatgagg atgagctggt cttgagcctg cgggggaggg ctgagga
//
LOCUS       DAOSGP010000170         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_32445_length_2913_cov_16.973408, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000170 DAOSGP010000000
VERSION     DAOSGP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32445_length_2913_cov_16.973408"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1393
                     /locus_tag="PLV49_05445"
     CDS             <1..>1393
                     /locus_tag="PLV49_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrophobe/amphiphile efflux-3 (HAE3) family
                     transporter"
                     /protein_id="HOG98324.1"
                     /translation="KHVTKPVIIALIITSIGFVSLLTSSIPMIRDFGLLCLIGLILCF
                     LSALFVGVTILYIAVGKRLNHQPTDELSTQDTNADDRGEVDPRGEGTIFERMLTEISG
                     FCIERWQLVLALALLLSLAGIYSDTQVPVDTDFKNYVPQDLPPLIDYWHMSETFGGTD
                     SIQLLVQADDVTDPQVLEWMDEFSIYLKGSRDQILGYTSIASLTKEANQGTLPKDRTI
                     IRMLIDALPANWRDIYLDGHNTAIIDLNVGQTITNLGTEGSDRLMKEIEKDIAWNPPP
                     AGVSVRMTGDAVVMTTVITALTRGRTEMTLFGLLLIFIVLLAIYRDLIKALLPVLPML
                     VVIGWMGGVMYISGMKYTPLTACLGALILGVGSEYAILMMERFYEELEKIGDPRKALS
                     TATRAIGSALIASGLTTIFGFAALISSPFLITNNFGMVTVLAIVFALATTFTVFVVLI
                     YRMELRRELVKNAI"
     assembly_gap    1394..1493
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1494..1990)
                     /locus_tag="PLV49_05450"
     CDS             complement(<1494..1990)
                     /locus_tag="PLV49_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98325.1"
                     /translation="MFEELLELYKTKAEEIERDRREAQAMIMLVSAIDKMSQDMDSKK
                     SDVELLGRLIDQVDRLSKEVSDLKSQFRTRQARPSAAVESGSGDGKALDNHARSLIDI
                     MAEKPGRYTTSELVDMLGLNKTTVISVMKRALELDPKHVKMSQGKRRKLYLSYVSDED
                     TQKVES"
     gene            complement(2070..>2913)
                     /locus_tag="PLV49_05455"
     CDS             complement(2070..>2913)
                     /locus_tag="PLV49_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG98326.1"
                     /translation="VMDVAGTIMRMYRVAKDIPGDIILEKVVTWELIMEKSGRALVVP
                     QIDPDTIISCNPDHPIRVLVLGREDCIKISCSSSQISPDEVLRILKSSPVKMRDLQEA
                     CAIVKAKCPGNYRTAGFIVDRDHGEIAYAISTGGIPFPGLERVLLDLKAMGADVFLAS
                     GDSRRSLNSLDDLGIDPSRMNPVANPWRKKEIVAELKERYGQVVMVGDGLNDIYALKA
                     ADLGVLTVEQHTRPSPRLLDSASVIIKSIRDLPRVIKESGFISQDESIPSRQGELQEK
                     YYIP"
BASE COUNT          644 a          772 c          699 g          698 t
ORIGIN      
        1 caaacacgtc accaagccgg tgatcatcgc cctgatcata acctccattg gctttgtctc
       61 tctgctcaca tcctccatcc ccatgatcag ggatttcgga ctgctctgct tgatcggact
      121 gatcctctgc ttcctctctg ccctcttcgt gggggtgact atcctttaca ttgcggtggg
      181 aaagcgcttg aaccatcaac caacagatga gctttcgaca caagatacga atgcagacga
      241 caggggagaa gtcgacccga ggggagaggg gacgatcttt gagaggatgc tgacggagat
      301 ctcaggcttt tgcattgaga ggtggcagct cgttttggct ttagccctac ttctttccct
      361 ggccggcata tattcagata cccaggtccc cgtggacacc gacttcaaga attatgtgcc
      421 ccaggatctg cccccactga tagattactg gcatatgagc gagaccttcg ggggcactga
      481 cagcatccag ctgctggtgc aggccgatga tgtcaccgat ccgcaggtgc tagagtggat
      541 ggatgagttc tccatctatc tgaaaggctc cagagatcag atattgggat atacgagcat
      601 tgccagcctg acaaaagagg caaaccaggg aactctcccc aaggatagga caattatccg
      661 aatgctaatc gatgcccttc ctgccaattg gcgggatata tacctggatg gacacaacac
      721 cgccataatc gacctcaatg taggccagac cataaccaac ctgggcaccg agggctcaga
      781 ccggctgatg aaagagatcg agaaagatat cgcctggaat cctcctccag caggggtctc
      841 ggtgagaatg actggagacg cagtggtgat gaccacggtc ataactgccc tgaccagagg
      901 aaggacggag atgaccctct tcggcctctt gctgatattc atagtgctcc tggcgattta
      961 ccgggatctg ataaaggcct tgcttcctgt gcttcccatg ctggtggtta taggctggat
     1021 gggtggtgta atgtacatat caggcatgaa atacaccccc cttactgcgt gcctgggggc
     1081 tctgatcctg ggagtgggat cagagtatgc catcctgatg atggagcggt tctatgagga
     1141 gctggagaag atcggcgatc ccagaaaggc cctctccaca gccaccaggg ccataggctc
     1201 ggcattgata gcctcagggc ttaccaccat attcggattt gccgccttga tatcatcccc
     1261 atttctgatc accaataact tcggcatggt gaccgttctg gccatagtgt tcgccctggc
     1321 caccaccttc accgtatttg tggtgctgat ctaccgcatg gaattgagaa gagagctggt
     1381 gaagaatgca attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngattcta
     1501 ccttttgcgt atcctcatcg gagacatagg acaaatagag cttgcgcctc ttgccctggc
     1561 tcatcttgac atgcttcgga tcaagctcta aagcgcgctt catcacgctt atgactgttg
     1621 tcttattcag accgagcatg tccaccagct cgctggtggt gtatctgcct ggcttctccg
     1681 ccatgatatc gatcagagat cgggcatgat tatccagggc cttaccatcg cctgagcctg
     1741 actccactgc agcagaaggc cgggcctgcc tggtgcgaaa ctggctcttc aggtcggata
     1801 cctctttgga taagcggtca acctgatcga tcagcctgcc caaaagctcg acatcgctct
     1861 tctttgagtc catgtcctgg gacatcttgt cgatagcgga gacgagcatg atcatagcct
     1921 gtgcttctcg acgatctctt tcaatctctt cggcctttgt tttgtatagc tctaaaagct
     1981 cttcgaacat ttattggcta ctcctagtat cttctagttc aatctcattt cgaagcgaat
     2041 taaaaggttg ccggacagag ggtcaactgt caaggtatat aatacttttc ttgtagttcg
     2101 ccctgtcggg aaggaataga ttcgtcctgg ctgataaatc cagattcttt aatgactcgg
     2161 ggaaggtcac ggatgctctt gatgatgaca ctggcagaat ccagcaaccg cggagagggg
     2221 cgagtgtgct gctctaccgt cagaaccccc aggtctgccg ccttcaaggc atagatatca
     2281 ttcaagccat cgccaaccat gaccacctgg ccataccttt ccttgagctc tgctacaatc
     2341 tccttcttcc gccaggggtt ggcgactgga ttcattcgcg atggatctat gcccagatcg
     2401 tccaggctgt tcaggctcct tctgctatct cctgaggcca gaaaaacatc cgcgcccatg
     2461 gcctttaaat caagcagtac cctctccaat ccaggaaatg gaatgccacc agtgctgatt
     2521 gcataagcga tctccccatg atcacggtca acgatgaatc ctgcagtgcg atagttcccc
     2581 gggcatttcg ctttcactat tgcacaggcc tcctgcagat cgcgcatctt gaccggagag
     2641 ctcttcagaa tcctcaagac ctcatctggg gagatctgag aggatgagca gctgatcttg
     2701 atgcaatcct cgcgacccaa gaccagcacc ctgatgggat ggtcgggatt gcaggatatt
     2761 atcgtatccg gatcgatctg aggcactacc agtgcccggc cactcttctc catgataagc
     2821 tcccaggtga cgaccttctc caggataata tctcctggta tgtccttggc cacgcgatac
     2881 atcctcataa tggtccctgc tacatccatg acc
//
LOCUS       DAOSGP010000171         2901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_32634_length_2901_cov_20.223823, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000171 DAOSGP010000000
VERSION     DAOSGP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32634_length_2901_cov_20.223823"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLV49_05460"
     CDS             complement(<1..605)
                     /locus_tag="PLV49_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOG98327.1"
                     /translation="MDRSQAGKALAGALDGFIGQDSVVYALPRGGVVLGYEVARHLDA
                     PLDLLITRKIGYPGSRECAVCAISEDGEMICDSLGASRLDPVWLGVRAEEEMEEANRR
                     RRAYLEGRPPLNAGGKVAIVTDDGMATGLTVLLAIMVLKKQHPKRIVVAVPVSSMEAA
                     ERIRREADELFALDVPPDFCSVSEHYEEFAQLEDEDVIRLL"
     gene            669..1607
                     /locus_tag="PLV49_05465"
     CDS             669..1607
                     /locus_tag="PLV49_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOG98328.1"
                     /translation="MLISEAEFGSIVSRYKSVLYLCHRNADPDAIGSAFALAGAFGGT
                     VGAIDDLSRTGEALSDIIGAHILIDPSEEGWDLIVVVDTSVRLQLGRIQLARYGLIDH
                     HQDEGLTREAEFYIQRPAKSTAEIVWKILKDNGRQPSREMALGLMAGIISDTGRFKRA
                     SAESFMAASELLEAGGFDYEEALQTLSVAPAISQRIAVLKAASRTKIERQGEWLIAAS
                     RINSFEGSAAAALIDLGADVAFVAGRHGDRVRISARSSRKAAKAGLNLNQILGDIGRS
                     HGGDGGGHNSAASFDARGDPEALVQECRNRVAELLP"
     gene            complement(1618..2838)
                     /gene="pgk"
                     /locus_tag="PLV49_05470"
     CDS             complement(1618..2838)
                     /gene="pgk"
                     /locus_tag="PLV49_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOG98329.1"
                     /translation="MKDYLTMEDVKLDNKRVLVRVDFNSPMDPSGNILDDKRIKSHLD
                     TLRALEDCRVVLMAHQSRAGKKDYTTMEAHARSATRLLRRDVAYIDDIFGSHAREAIR
                     SIMPGEVLLLENTRFYAEENMNRTPADHAKSHMVKRLAPLFDLFINDAFAVSHRSHCS
                     VVGFTEVLPSIAGLLMDKEITALDKGLKGDEHPTIYSLGGTKADDSIKVIENVLRRGG
                     ADKILTSGVVATVFLMAAGINIGEANRKFVEDQEYLEQIPIAAKLLAEYPDKIAMPVD
                     VALNKNGERIDVAVSALSNDLPIADIGLETIVDYSKELKDARVTVMNGPAGIFEQEKF
                     KLGTSELLKASAESDYSIAGGGHSVAAIEQLGLEPKFSHVSMGGGASITYLSGEPLPG
                     IEALKKYALKVRKA"
BASE COUNT          639 a          820 c          786 g          656 t
ORIGIN      
        1 ttgagcagac ggatgacatc ctcatcctca agctgtgcaa actcctcgta atgctcggac
       61 actgaacaga aatcaggggg cacatccagg gcgaaaagct catcggcttc tcttctgatc
      121 ctctctgctg cctccatcga cgagacggga acggcaacca ctatcctctt aggatgctgc
      181 tttttcagca ccataatggc caggaggacg gtaagcccag tggccatgcc gtcgtctgtc
      241 actatggcca ccttgcctcc tgcattcagg ggcggtctgc cttcaagata ggctcttcgc
      301 cgtctgttcg cctcttccat ctcctcttct gcccggacgc cgagccatac gggatccagg
      361 cgggaagcgc ccaagctgtc gcatatcatc tccccatcct cggatattgc gcagacggcg
      421 cattcccggc tgccgggata gccgatcttt ctggttatga gcagatccag tggagcatca
      481 aggtggcggg ctacttcata tcccaggacc accccacctc tgggaagggc atagaccacc
      541 gaatcctggc ctataaaccc gtccagggcc cctgccagcg cctttcctgc ctgggatcta
      601 tccataaaga gcataaaata ttgtctggcg ggaaagaact tatctctttg gatttgacca
      661 attctcttat gctcatctca gaggccgagt tcggctccat cgtcagcaga tacaaaagcg
      721 tcctgtacct gtgccaccgc aacgccgacc ctgatgccat agggagcgcc tttgccctgg
      781 ccggggcctt cggtgggaca gtgggggcga tagacgacct gagccgcacc ggagaggctc
      841 tatctgatat cataggagca cacatactga tcgatccctc agaagagggc tgggatctga
      901 tcgtggtggt ggacacatcc gtccggctgc agctgggcag aattcagctt gccagatatg
      961 gactcataga ccaccatcag gatgaagggc tgacccgaga ggcagaattc tatatccaaa
     1021 ggccggccaa atccactgcg gagatcgtct ggaagatcct gaaggataac ggacggcagc
     1081 cgagcaggga gatggccctg ggtctaatgg cagggattat ctcggatact ggacggttta
     1141 agcgggccag cgcggagtca tttatggcag cctcagagct actggaggca ggaggatttg
     1201 attacgaaga ggcattgcag accctatccg ttgccccggc catctctcag aggatagcgg
     1261 tcctcaaggc agcctcccgg acaaagatcg agcgccaggg agaatggctg atagccgcct
     1321 ccaggataaa ctccttcgag ggctcagcgg cagcagcgct gatcgacctg ggggcagacg
     1381 tggcctttgt tgccggaagg catggtgatc gggtcaggat cagcgcccgg tccagccgca
     1441 aagcagcgaa agccggcttg aacctcaacc agatcctggg tgacatcggc aggtctcatg
     1501 gaggtgacgg cggaggccac aattcagcag cctcctttga tgccaggggc gatcctgaag
     1561 ccctcgtgca ggaatgcaga aatagggtgg cagagctgct gccctgatat ttcttttcta
     1621 agccttgcgc acttttagag cgtacttttt cagggcttca attcccggca ggggctctcc
     1681 cgagaggtac gtgatgctcg caccgccgcc catgctcaca tgagagaact tgggctccag
     1741 ccccagctgt tcgatggcgg ccacgctatg ccctccgccg gcgatggagt agtccgactc
     1801 ggctgaggcc ttcaggagct cagatgtccc caatttgaac ttctcctgct caaatatccc
     1861 tgccggcccg ttcatgactg taacccgagc atcctttagc tctttggaat aatcgactat
     1921 ggtctccaga cctatgtccg ctataggcag atcgttggaa agggcggaga ctgcgacgtc
     1981 gatcctctct ccattcttat tcagggccac atccaccggc attgcgatct tgtccggata
     2041 ttctgccaga agcttagctg caatcggtat ctgctcaaga tactcctgat cctctacgaa
     2101 ctttcgattg gcttcaccaa tattgattcc tgctgccatc aagaaaacag tggctacaac
     2161 ccccgaggtc aaaatcttat ccgctccgcc gcgccgcagc acattctcta tcaccttgat
     2221 ggagtcgtcc gctttggtac ctcccaagga gtagatcgtg ggatgctcat cgcccttcag
     2281 ccctttatcc agagcagtga tctctttgtc catcagaagg ccggcaatac tgggcaggac
     2341 ctcggtgaat ccgacaacag agcaatgaga gcgatgggag acggcaaaag cgtcatttat
     2401 gaaaagatcg aatagaggag ccagcctctt caccatatgg cttttggcat gatcggcagg
     2461 cgtgcggttc atgttctctt ctgcataaaa acgggtgttc tccagtagaa gaacctcccc
     2521 tggcattatg gatcttatgg cctctcgagc atgagagccg aagatgtcat cgatataggc
     2581 aacatcgcgt cgaaggagtc tggtggcaga gcgggcatgg gcttccatgg tggtatagtc
     2641 cttctttccc gccctgctct gatgggccat gaggaccacc cggcaatcct ccagagcgcg
     2701 caaagtatct aaatggctct tgatccgctt gtcatccagg atatttccgc tcgggtccat
     2761 gggggagttg aagtcgactc taaccaggac ccttttatta tcgagtttga catcttccat
     2821 cgtgagataa tccttcaact aaagttcctc ccggtgtgtt aggggttatc ggaaggagta
     2881 taaagtcgtt gctgatttgg g
//
LOCUS       DAOSGP010000172         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_32961_length_2882_cov_18.708171, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000172 DAOSGP010000000
VERSION     DAOSGP010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32961_length_2882_cov_18.708171"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..584)
                     /locus_tag="PLV49_05475"
     CDS             complement(3..584)
                     /locus_tag="PLV49_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98330.1"
                     /translation="MTGMDTIPSVWRYGLALLIVIAGFAAFTWYLTTGISSSFGGLSQ
                     MAAPGELELDLKEPGEYTIFYENKSYFNGSFYMTAAQIPGLQIVVMEKSSGRRLRTYP
                     APTGSTYSIGGRSGQSFMAFRAEDAGIHVINASYPSGPGPKVILAVGNEVMEDLLRMI
                     MVSMTLILVSLLIAAIITYTTYKGRKKALEAER"
     gene            complement(637..1197)
                     /locus_tag="PLV49_05480"
     CDS             complement(637..1197)
                     /locus_tag="PLV49_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98331.1"
                     /translation="MSLVLHSGTMWDCSYKAGHLSGSLRGNSAGRNLNILRVVVIQKL
                     SWMVKTMKKFGMAVLIMLMAALSLTAIAYSLDGVEYRPAEKAMEKLFEKGIGGPWVGG
                     DPFTSDIFKSDGTQMGQKQTTSIQFPLSSCQLMGFLKTDSGLIIASDKDNSLKLSPGP
                     GNYPVYGCFEDGKLMGLFIDFRFLSI"
     gene            1299..2588
                     /locus_tag="PLV49_05485"
     CDS             1299..2588
                     /locus_tag="PLV49_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOG98332.1"
                     /translation="MKGLADFHLAGKNQGTIALTGTFCATIMGASTTIGMAGLGYSRG
                     LPGSWWLLSGTIGMLLLSVLLAEKIRATGCSTLPQLVGSFWGERARAAASILIAISWI
                     GVIAVQIIASGNILLAVFGGDMSHYMVACTIVFVLYTAYGGKVSVIRTDMFQILIIIF
                     GMIALFWQALKAAGPLPLIQLSFPTSAEMNCWEVISMILVVGSAYLIGPDMYSHIFSS
                     RSSKEAKASAFISAILLIPLAFLITSLGILSRYLYPTITPEQAIPSLMAGLLSPAIVG
                     LVAAALLAAFMSSADTCLMTATSILTFDIYGEVYRRVRSNADPNVIQDHMMKVSRIAV
                     VAIGASALILAVSNPDIIKTLLIAYTVFTSGLLVPVLAGFCKDRLGLTCNGAISAIAG
                     GGFIAIILGQSYPLLGMTASAVILFAASWLERWLARR"
     gene            complement(2659..>2882)
                     /locus_tag="PLV49_05490"
     CDS             complement(2659..>2882)
                     /locus_tag="PLV49_05490"
                     /inference="COORDINATES: protein motif:HMM:NF028518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOG98333.1"
                     /translation="TAKQSSPQFIPKLKGKRVLIRLTSGGQPVTGTIESFNSYEILLQ
                     TAKGELLVFKHAISTIEVVDEPRGYRPAV"
BASE COUNT          695 a          751 c          701 g          735 t
ORIGIN      
        1 ttttatcttt ccgcctcaag cgccttcttt ctccccttat aggttgtgta ggtgataatg
       61 gcggcaatca acagcgagac caagatcaag gtcatagaga ccattatcat acgaagcaaa
      121 tcctccatga cctcatttcc cactgccagg ataaccttcg gcccgggccc agatgggtag
      181 gacgcattta tcacgtggat tcctgcatct tccgctctga aagccatgaa agactggcca
      241 gagcgcccac cgatggaata ggttgagccg gttggtgcgg gataggtccg gagccttcgg
      301 ccggaagact tttccatcac cacaatctga agtcctggta tttgcgccgc ggtcatatag
      361 aagctgccat tgaagtagct cttattctcg tagaagatgg tatactcgcc tggctccttg
      421 agatcgagct ccagctcacc gggtgcggcc atctggctca ggccgccgaa agagcttgat
      481 attccggtgg ttagatacca ggtaaatgcg gcgaatcctg caatcacaat caacagggca
      541 aggccgtatc gccaaacgct gggtattgta tccatgcctg tcatgagtga atgccttgca
      601 tcataaaaag gctatgcgag agcccgtaac ggaccctcag atagataaga acctgaaatc
      661 gataaagaga cccatcaatt tgccatcctc gaagcatcca tagacaggat agttgccggg
      721 accgggactc agcttgaggc tgttatcctt atcggaagcg attatcagtc cgctgtcagt
      781 ctttaagaat cccatcagct ggcagcttga aagtgggaac tggattgaag tcgtctgctt
      841 ttggcccatc tgagtcccat cggacttgaa gatgtcggac gtgaatgggt ccccgcccac
      901 ccatggaccg ccaataccct tttcgaagag tttctccatc gccttttcag caggccgata
      961 ttcaacgccg tccaatgaat aagcaatcgc ggtcagcgat aatgccgcca tcagcatgat
     1021 cagcacagcc attccgaatt ttttcattgt tttcaccatc cacgataatt tttgaatgac
     1081 aactacgcgc aagatattta aatttcttcc ggcagaatta ccccggagag atccagaaag
     1141 atgaccagcc ttataactgc agtcccacat tgtcccggaa tgcaatacaa gagacataat
     1201 cagccatatg ctttccagga ggatttacga atcacgcggt catagcgata gcagtctata
     1261 tgctggccat ttttatcata ggaacttggc gccatcgatt gaaagggctg gcggacttcc
     1321 atcttgctgg caaaaaccag gggaccatag ccctcacagg caccttttgt gcaacgataa
     1381 tgggagcctc cacaaccata ggaatggctg gcctgggata cagccggggg cttccaggtt
     1441 cctggtggct gctctcgggc acaataggaa tgctgttgct gtctgttctt ctggctgaaa
     1501 agataagagc caccggatgt agcactctgc cccagcttgt cggctccttc tggggggaga
     1561 gagcaagggc tgctgcctct attctcattg ccatttcctg gataggcgtg atcgccgttc
     1621 agatcatagc ttctggaaat atcctcttag cggtctttgg tggggatatg tcccattaca
     1681 tggttgcctg cactatagta ttcgttctct atactgcata tggaggaaaa gtttccgtca
     1741 taagaactga catgtttcag atcctgataa taatttttgg aatgatagcc cttttctggc
     1801 aagccttgaa ggccgcggga cccttgcccc tcatccagct gagctttccc acatctgctg
     1861 aaatgaattg ctgggaagtg atctccatga ttttggtcgt aggttcagct taccttattg
     1921 gcccggatat gtattcgcac atcttctcct cccgcagctc aaaagaggca aaggcgtctg
     1981 cattcatttc cgcgatcctc ctgatacccc tggcattcct gatcacctca ctgggaatat
     2041 tatccagata tctctatccg acaatcaccc cagagcaggc aattccatcc ctgatggcag
     2101 ggctcctctc gccagcgatt gtaggcttgg tggccgcagc tctcctggct gcctttatgt
     2161 cctcagccga tacctgcctc atgactgcca catctatcct caccttcgat atttatggag
     2221 aggtgtacag gagggtgagg agcaacgcag atccaaacgt cattcaggac catatgatga
     2281 aggtctcaag gattgcagta gtggcaattg gagcatctgc tctaattctt gcagtctcca
     2341 atcccgacat catcaaaacc ctgctcatcg cctacacagt attcaccagc ggccttctcg
     2401 tgcctgtgct cgccggattc tgtaaagata gactcgggct tacatgcaat ggagctatct
     2461 cggccattgc aggaggaggg ttcattgcca tcattctggg ccagagctat ccactgctag
     2521 gcatgacggc ctctgcggtg attctatttg cagcaagctg gctggagagg tggctggcaa
     2581 gaagataagc aaattcagcg aagagccgaa aaaataggag aggatgattc tatttgcaga
     2641 tgtctactgc tatcgtcatc agactgcagg cctataccct ctgggctcgt ccacgacctc
     2701 gatggtagaa atagcatgct tgaataccag tagctctccc tttgcagtct ggagcaggat
     2761 ctcatatgag ttgaatgact caatggtccc agtaaccggc tgaccaccgg aggtcagacg
     2821 gatcaatacc cttttgccct ttaattttgg tatgaattga ggcgaggatt gctttgcagt
     2881 gg
//
LOCUS       DAOSGP010000173         2846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_33542_length_2846_cov_19.584737, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000173 DAOSGP010000000
VERSION     DAOSGP010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33542_length_2846_cov_19.584737"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..1840
                     /locus_tag="PLV49_05495"
     CDS             452..1840
                     /locus_tag="PLV49_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOG98334.1"
                     /translation="MLIASVACAATISVSSDLQAAINAAQPGDTLLVAPGIYEKISIG
                     KSLNLVGNGAVIQAGERDACVNIEASDVSISGFLVRNGFYGIKLNNVRGCTVANNTVI
                     RCNQPGIALLFSDGNIIQGNNASFNGLGGEGWYGIYLSNSNDNLILDNVAYGNGAYGI
                     NLFPSCSNNTIRGNVLQGNMYGLYMFTDCSNNIIEYNDLSRNTNSGLDLRFDCTDNLI
                     RNNSMIENVVAGLTLLDSGYNSISGNLIEANGRYGLQIQSNSNGNTIIDNTISDSQTG
                     IFLDSSQNMIYRNKISYNVIQAEDRGENSWNASYPLGGNLWSDYHGSDEYQGQEQNVL
                     GADGFGDLPYEIDSSSVDRYPIMGDQVRQISIIEKALNPTQARVGDDIKVTVRMEALY
                     DLNQLTVRAYQSGEEAKGYGRLVASGDAYQGSFSTALLDPGEYDLVLRVNDVRGFELE
                     ETLGSFSVSPRS"
     gene            1894..2796
                     /locus_tag="PLV49_05500"
     CDS             1894..2796
                     /locus_tag="PLV49_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG98335.1"
                     /translation="MRVIRTAGCSLLLAALFMMAFIWNATPVAGECASCKAAGSSAEQ
                     GALNQQVNRSDMAREMGFSTILISPEEIKILDSSDIIIDISPRSTEYIPGAISIPYDR
                     FIDPAGRLEPIGEMTRILGDAGVSENDSIIIYGECQPCGGGPSAATYVFWMLKFLGHE
                     DVRLLDGGIDDWVAASNPTWSEPARLAPRNYTPAIDADLLATYDFVCSGIPQIIDART
                     GRDFEAGSIPGAINIPYTSVLDGKKIKNESELEEIFSDLQRERPVVVYTNTGVKASMI
                     WLPLTLLGYDAKVYAWQDWKADQW"
BASE COUNT          745 a          735 c          739 g          627 t
ORIGIN      
        1 catcgtcatc gtcctgggaa gacacccaca gtagatcgct atcgccatag ctttcctcct
       61 cttcaataga attgacgtag gtatccacat cgattctcgc ttcaaagctc cctgcgctgc
      121 cagcggccgc cagcaggaga agtgtgccaa taatcgcaag ccagtatctc aaacgctatt
      181 cctccattga atttaaccaa tactgaatgt acttgtcaat tgataaaaaa agcttacctt
      241 catatgtagc ctatattaga taagccatga atctttataa aatagatcga tcatctagag
      301 atggatcgct atgagcctca aaatgaaacc atcattaacc catcccagtg ccacgggaaa
      361 atgccatgct ccggctttat caaatgcata tcatccttcg cgcggcatgg gtcgcccgac
      421 catttgggcg gtatcccttc tttatgcttt tatgctgatc gcttccgttg cctgtgccgc
      481 caccatatcc gtcagctccg atctgcaagc ggcaatcaac gccgcccagc ctggcgacac
      541 cctcctggtg gccccgggga tttacgagaa gatatccata ggaaagagcc tcaacctggt
      601 aggcaatggg gcagtcattc aggcagggga gagggacgcc tgcgtgaata tagaagcgag
      661 cgatgtcagc atatccggct ttctcgtcag gaacggattt tatggcataa agctgaacaa
      721 tgttcggggc tgcactgtgg ccaacaatac cgtcatccgg tgcaaccagc cagggattgc
      781 cctccttttt tccgacggca atatcatcca gggaaacaac gccagcttca acggactggg
      841 gggtgaaggg tggtatggaa tatacctctc caactccaat gacaatctga tccttgataa
      901 tgtggcctat ggcaatggcg cctacggcat aaacctcttc ccctcctgca gcaataacac
      961 catccggggc aacgtccttc aaggaaatat gtacggcctg tacatgttca cagattgcag
     1021 caataacata atagaataca atgacctctc ccggaacacc aactccggcc tggacctccg
     1081 cttcgactgc actgacaacc tgatccgaaa taacagcatg atcgagaacg tagtcgccgg
     1141 cctgaccctc ctggactctg gatataacag catatccggc aatctgatag aggcaaatgg
     1201 gcgctatggc ctgcagatcc agagcaattc gaacggcaat accatcatcg ataacacaat
     1261 ctcggacagc cagaccggca tcttcctgga ttcgagccag aacatgatct acagaaataa
     1321 gatcagttac aatgtgatcc aggcggagga tagaggagag aattcgtgga atgccagcta
     1381 tccgctgggg ggcaacctgt ggagcgacta ccatgggagc gatgagtacc agggacagga
     1441 acagaatgtt ctgggtgcgg atggatttgg cgacctgccc tatgagatag acagttcctc
     1501 cgtagatcgt tatcccatta tgggcgatca ggtccggcag atctccatca tcgagaaggc
     1561 tcttaatccc acccaggcca gagtgggaga cgatataaag gtcaccgtgc gtatggaggc
     1621 tctctatgac ctcaaccagc tgacggttag agcatatcag tcgggagaag aggcaaaggg
     1681 ctatggccgc ctggtagcct ctggggatgc atatcaggga tccttctcca ccgccctgct
     1741 cgatccgggc gagtacgacc tggtcttgag ggtaaatgat gttcgtggat tcgaactgga
     1801 ggagacttta ggaagcttct cagtctcccc caggagctga tgagccttct attgcctgaa
     1861 gacgaaaaat ttaagatgtt caagagtctt gccatgagag taatcagaac cgcgggctgc
     1921 agcttgttgc tggcagcctt attcatgatg gcgttcatat ggaatgcaac acctgtggcc
     1981 ggagagtgcg catcctgcaa agctgccggc tcgagcgcag agcagggtgc gctcaatcaa
     2041 caggtcaatc gatccgatat ggcccgggag atgggatttt ccacaatttt gatttcacca
     2101 gaagagatca aaatcctgga tagctccgac ataatcatcg atatcagccc cagatcgaca
     2161 gagtacattc caggagcgat aagcattccc tatgacaggt tcatcgaccc tgcaggaagg
     2221 ctcgagccga tcggcgagat gacccggatt ttaggggatg caggcgtatc cgagaacgat
     2281 tcgatcatca tttatggtga atgccagcca tgcggcggcg ggccctcggc agccacctac
     2341 gtcttctgga tgctgaaatt cttggggcat gaggatgtca ggctcctgga cggggggata
     2401 gacgactggg tggcagcgtc gaatcccacc tggtctgagc ctgcacgcct tgcgcccagg
     2461 aattatactc ccgctataga tgccgatctt ctggcaacat atgatttcgt ttgcagcggc
     2521 atcccccaga taatcgatgc ccgaacgggg agggattttg aggcagggtc gataccagga
     2581 gcgatcaata tcccctatac aagcgttctg gatggcaaga agataaagaa tgagtcagag
     2641 ctggaagaga tattctcaga tctgcaaaga gaaaggccgg tggtggtcta caccaatacc
     2701 ggggtcaagg cctccatgat ctggttgccc ctcacccttc tcggctatga cgccaaggtc
     2761 tacgcctggc aggactggaa agcggatcag tggtgaaatg acaccagcaa atataagaat
     2821 atcgaaaagg atagctggag ttaaga
//
LOCUS       DAOSGP010000174         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_33966_length_2822_cov_14.995663, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000174 DAOSGP010000000
VERSION     DAOSGP010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33966_length_2822_cov_14.995663"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLV49_05505"
     CDS             <1..198
                     /locus_tag="PLV49_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HOG98336.1"
                     /translation="ASNNFELAIAVAVGVFGIASQEAFAAVIGPLIEVPVLIALVNVA
                     LKLRDKYYPSAQEKKIFCERT"
     gene            218..1015
                     /locus_tag="PLV49_05510"
     CDS             218..1015
                     /locus_tag="PLV49_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG98337.1"
                     /translation="MTGSPAEVKLVSNAMANATRRKIMAMLVEKERTKEEIEQAVGGT
                     MLDYHLQMLGQAGLADMKEDRVVITDFGTNFMQAKSDKPAETKKDLAGTRPLQVVDLR
                     QLLPCIADSSKFRIIARFDPPLEGALKLLEPLFPRARYSDRIGALIIQRGNILITIYS
                     TGNVTMTMIKSEAEAREVLEDLKKTINEAIIKGVTPVPREKVKVDHAEIYQYLPRTDC
                     QICGEQSCYAFAIKLVGRETEIDKCTPLLEPRYATNLEHIRTLLEYL"
     gene            1037..1399
                     /locus_tag="PLV49_05515"
     CDS             1037..1399
                     /locus_tag="PLV49_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOG98338.1"
                     /translation="MKTLTIILTDGPYISQYADMAYKIASRALNVFQVNIFLYLDAVH
                     IPKSGQNPSQFKNTGRLFQELADRGAVVRACARCAAARGYQAEEDGSCPDYLQGIKIT
                     SLYDLAEMLEKSDRVIAL"
     gene            1396..1737
                     /locus_tag="PLV49_05520"
     CDS             1396..1737
                     /locus_tag="PLV49_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOG98339.1"
                     /translation="MRSIFYLLDSAPYGSEKAYGVLNATAVSIKMDVTLGLYADGVYL
                     ALAGQNSCLLDVPSLSDILYAYPELRVLAHEPSLGERGLLSRGLVERVELVDEEDFVK
                     QALAADGLIAL"
     gene            1748..2044
                     /locus_tag="PLV49_05525"
     CDS             1748..2044
                     /locus_tag="PLV49_05525"
                     /inference="COORDINATES: protein motif:HMM:NF016003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrH/TusB family sulfur metabolism protein"
                     /protein_id="HOG98340.1"
                     /translation="MANRDIFLLTKPPQSERTELCLRLMEHGDDSVLYLAGDGVYNLL
                     DEALMGKWPGRVMACKEDMEARGLQAGEGLNVPEDFYQLLVEDMVVEGSRIYSF"
     gene            2079..2363
                     /locus_tag="PLV49_05530"
     CDS             2079..2363
                     /locus_tag="PLV49_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98341.1"
                     /translation="MDGVLARKNGKKDCYYEWTVGCHQVKVGKCSMEVKGLYELFQNY
                     SKKGMTAEEIDGSEMIKDVRRQNFIPEDIEDLYEEALIKEYKRYFESRKK"
     gene            complement(2402..2731)
                     /locus_tag="PLV49_05535"
     CDS             complement(2402..2731)
                     /locus_tag="PLV49_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98342.1"
                     /translation="MLLCECGEIIDNCTFKDYIETSANPSTPTIGHQKCGNIFNFVDG
                     KMPKRYSSKIELKSIAMRFAEKNKMSIETIESLLIEVDRMKSSGNLSDGEILIAAIKK
                     CCKDRRY"
BASE COUNT          720 a          663 c          782 g          657 t
ORIGIN      
        1 gccagcaaca acttcgagct ggccatcgcc gttgccgtgg gcgtctttgg catcgcctcc
       61 caggaggctt tcgctgcggt catcggcccc ttgatcgagg tgccggtcct gatcgccctg
      121 gtgaacgtgg ctttgaagct gagggacaaa tactatcctt ccgcccagga gaagaagata
      181 ttctgtgaac gcacttgaaa tccggatgtg atataaaatg actggatcgc ctgctgaggt
      241 aaagctcgtt agcaatgcca tggccaatgc tacgagaaga aagatcatgg ccatgctggt
      301 ggaaaaggag aggacaaaag aagagataga gcaggcagtg ggaggtacca tgctcgacta
      361 ccacctgcag atgctcgggc aggcgggcct ggctgacatg aaagaggaca gggtcgtcat
      421 cacggatttc ggaacgaact tcatgcaggc caagtccgat aagccggcag aaacgaaaaa
      481 agacctggct ggcacaagac ctctgcaggt cgtcgatctg cgtcagctac tgccctgtat
      541 cgccgacagc agcaagttca ggataatcgc ccgcttcgat ccgcccctgg agggagccct
      601 caagctattg gagccactct ttccccgagc caggtactct gataggatag gcgctctgat
      661 cattcagaga ggtaacattc tgatcaccat ctattccacg ggcaatgtga ccatgaccat
      721 gatcaagagc gaggcagagg ccagggaggt tttggaggac ctgaaaaaga ccatcaatga
      781 ggccattatt aagggagtca ctcccgttcc gcgggagaag gtcaaagtgg accatgccga
      841 gatctaccag taccttcccc gcaccgactg ccagatatgc ggcgagcaga gctgctatgc
      901 ttttgccatc aagctggtgg gcagggagac cgagatcgat aaatgcacgc cgctcctcga
      961 gcccaggtat gccaccaatc tcgagcacat caggactctg ctggagtacc tttgaggatg
     1021 ccatgcaggt gagcagttga agaccttgac catcatactt acagacgggc cttatatctc
     1081 ccagtatgca gatatggcct ataagatcgc ctcccgggcg ctcaatgtgt tccaagtgaa
     1141 catattcctc tacctggatg cagtccatat tccaaagagc ggccagaatc cctctcaatt
     1201 taaaaatact ggaaggctat tccaggagct ggcggataga ggagctgtgg tcagggcctg
     1261 cgccagatgc gctgctgcca gaggctatca ggcagaggag gacggaagct gcccggatta
     1321 cctgcagggg ataaagatca ccagcctcta cgatctggca gagatgctgg agaagagcga
     1381 ccgagtgatt gcgctgtgag atcgatcttc tatcttcttg acagcgctcc ctatggaagc
     1441 gaaaaggcat atggcgtgct caatgcgaca gcggtgagca tcaagatgga tgtaacgctg
     1501 ggcctgtacg ccgatggcgt atatcttgcc ctcgccggcc agaactcctg ccttcttgat
     1561 gtgccaagcc tttccgatat cctctatgcc tatcccgagc tgcgggtcct ggcgcatgag
     1621 ccctccctgg gggagagggg cctcttgagc cgcgggctgg tggagagggt ggagcttgtg
     1681 gatgaagagg atttcgtgaa gcaggccctt gctgccgacg gtctgattgc actatgaatg
     1741 gtgaattatg gcaaatagag atatcttcct tctgaccaag ccgccccaga gtgaacggac
     1801 agagctgtgc ctgcgcctga tggagcacgg cgatgattcc gttctatatc tggcgggaga
     1861 cggggtttat aatcttctgg atgaggccct catgggaaaa tggcctggga gggtcatggc
     1921 ctgcaaagag gacatggaag cgagaggatt gcaggcggga gaagggttaa atgtgcctga
     1981 ggacttctac cagctcctgg tagaggatat ggttgtagag ggcagtcgga tatattcttt
     2041 ctaaaactgt atggaactcg gatgggatta acataccaat ggatggtgtt ttggcgagga
     2101 agaacggcaa gaaggactgc tactacgagt ggacagtagg atgccaccag gtcaaggtcg
     2161 gaaagtgttc tatggaagta aaaggattgt acgaattatt ccagaattac tctaagaaag
     2221 gaatgactgc cgaggagatt gacggcagtg aaatgatcaa ggatgtaaga aggcaaaact
     2281 tcattcccga ggacatcgag gacctgtatg aagaggccct tataaaggag tacaagagat
     2341 actttgaatc caggaagaag taaaatcctc aatagtggat gaagctttcc ttcggcatca
     2401 attagtatct cctatctttg cagcattttt tgattgctgc aataagtatc tcaccatcag
     2461 agagattccc ggaggatttc atcctgtcaa cctcgatgag caggctctca atggtttcaa
     2521 tgctcatttt atttttctct gcaaacctca tggcgatgct cttcagttca atctttgagg
     2581 aataccgctt gggcatcttt ccgtcgacga agttaaatat gtttccgcat ttctggtgtc
     2641 ctatggtcgg agtagatggg tttgcagatg tttctatata atctttgaag gtacaattgt
     2701 cgataatctc accgcattcg cacaagagca ttgaacccct cctggtaaat atcaaaagat
     2761 atctctaatt tattgaagtc tcgaagttca gatgcattct cctttaatgc aattgcttct
     2821 tt
//
LOCUS       DAOSGP010000175         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_34101_length_2815_cov_18.265942, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000175 DAOSGP010000000
VERSION     DAOSGP010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34101_length_2815_cov_18.265942"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..639)
                     /locus_tag="PLV49_05540"
     CDS             complement(28..639)
                     /locus_tag="PLV49_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98343.1"
                     /translation="MKKMILLILALCFGSLVGVTLSQNDEVDNAATMTVLKQDSGTNL
                     SQMNMTLYSVAMDMDAFSVGEAVKFTAPNPGWKLKQVRVAGWNGFDGNATSIPESENF
                     LLEVRDEKMNLLYRMADTQNAYFTFPAIILRAIDLPSVPVTDEFYVIFYDRGSMFIGM
                     ELENSTGSSYFYDSVYSELLPVEFTDQSNTTTSINWLIRAVGE"
     gene            852..1205
                     /locus_tag="PLV49_05545"
     CDS             852..1205
                     /locus_tag="PLV49_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HOG98344.1"
                     /translation="MSPKKMKGMLKSMGIDIDELEGVIEVVIRMPDKEIVIQNASVAI
                     MDAHGQRSYQISGDATERPLSGTEANEGPELEIPESDVDLVVAQTGANHEDARAALLE
                     AKGDLAAAILLLAPK"
     gene            1202..>2815
                     /locus_tag="PLV49_05550"
     CDS             1202..>2815
                     /locus_tag="PLV49_05550"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG98345.1"
                     /translation="MNDQKNRRNISDNKAIFVSEAREVIRDPVHGSISTDPLEWQIIR
                     SRPVQRLKGIKQLGLVEAVYPGANHTRFEHSLGTMHMAGRMAEHLGLSSEEVRKVRLA
                     GLLHDLGHSALSHAVEGVLGRNPEVQPLLKGKRASGHEEFTQDIISCHPFGQEAIRIA
                     ERDFGDADKLFAEVADIARGRIPPLGQIIVGDLDADRVDFLLRDSHHSGVSLGLVDTD
                     QILQSLTICKGRIVLVGQGDYRAEMSQTAAESMLIARAHHYNALIYHPSVQSIRAMLL
                     TALENALSRMNEDDAKRRIELFFREYTDPDLLRFIWEKGDDVSRDLLQRIKFGQEIPL
                     AARFDHRSLAPDIRMALSTIYRHGRMRKLFEDTLCKKYGALVSITAGSGVPRSMRTQA
                     DSFLYDESALAAGLVKSLTRQIAISFFSDDRPEISLDEVMEQAAKLLSFIRSESYLPI
                     DGLLLLFYALHNMLSERYGEKILVPRIRNITWLYRTVARLRDLGPGGLSGLFDYSFHS
                     EYGFPYSERLFEDIQILVANGMIYQDQRHY"
BASE COUNT          693 a          721 c          739 g          662 t
ORIGIN      
        1 ttcgtctgca ggactcagcc tgctctttca ctcgccaact gctctgatca gccaatttat
       61 gctggttgtg gtattgctct ggtctgtgaa ttcgactgga agcagctccg aatacacgct
      121 atcatagaag taagagctgc cggtagaatt ttccagttcc atacctatga acatcgagcc
      181 ccggtcatag aagatcacat agaattcatc cgtcacggga actgaaggca agtctatggc
      241 tcggagaatt atagcaggaa atgtgaagta agcattttgg gtatctgcca tcctgtagag
      301 gagattcatt ttttcgtctc tgacctcgag gaggaagttt tcagattcag gaattgatgt
      361 cgcatttcca tcgaagccat tccagcctgc taccctcacc tgcttcaatt tccagccggg
      421 atttggcgca gtgaacttca cggcctcgcc cacagagaat gcgtccatgt ccatagcgac
      481 gctatacaga gtcatattca tttgggagag atttgtgccg ctatcctgtt taaggacggt
      541 catggttgca gcattgtcaa cttcatcatt ttgagataga gtaactccca ctaaggatcc
      601 aaagcataga gccagaatca ataagatcat ctttttcatg cgatcaccct gaataaaagg
      661 tttatatttt ctctttataa agatgatgct gtattccgat gcaggaaacc actgacgagc
      721 tcgattccat tatatccaat cccctttgca cacaatagtg acagatatct ttacctacga
      781 taatatcgac tccttagatt agagtgattg acatgtttcc tggtttagga ggtttaggag
      841 gaaggggcgg catgagcccc aagaagatga aaggcatgct caagagcatg ggaatagaca
      901 tcgatgagct agaaggagtc attgaggttg tcatccgcat gcctgataag gagatcgtta
      961 tacaaaacgc ctctgtcgcg ataatggatg cccatgggca gcgcagctat caaatatccg
     1021 gcgatgccac tgagcgccct ctctccggaa cggaggcaaa cgagggaccg gagcttgaga
     1081 tccccgagtc agatgtggac ctggttgtcg cccagacggg tgcaaatcat gaagatgcaa
     1141 gagcggccct cctggaggca aaaggcgatc tagcggcagc aattttgctg ctcgcaccaa
     1201 agtgaatgat caaaaaaacc gccgaaatat cagcgataat aaggcgatct tcgtgagcga
     1261 ggcaagagag gtcattcgcg atccagtcca tgggtccatc tcgacagatc cgctggagtg
     1321 gcagatcatc cgatcccgtc cagtacaaag gcttaagggg ataaagcagc tgggcctggt
     1381 ggaagcagtc tatcccgggg ccaaccacac ccgattcgag cattccctgg gaaccatgca
     1441 tatggccggg cgaatggccg agcacctggg tctctcctct gaggaggtga gaaaggttcg
     1501 gttagccggg ctgctccacg acctgggtca ttccgctctc tctcatgctg ttgagggggt
     1561 cttaggccgc aaccccgagg ttcagcccct gttgaagggc aaaagagctt ccgggcacga
     1621 ggagttcacc caggatataa tctcctgcca tcctttcggc caagaggcca taaggattgc
     1681 agaaagggat ttcggcgatg cggacaagct ctttgcagag gtcgcagaca tagcccgggg
     1741 aaggattccg cccctggggc agatcatagt cggcgacctg gatgcagaca gagtggactt
     1801 tttgctcaga gactcccatc attctggcgt atccctgggc ctggtggata cagaccagat
     1861 actgcagtcc ctcaccatct gcaagggcag gatagtcctc gtcggccagg gtgactatcg
     1921 ggcagagatg tcccagacgg cagcggaatc aatgctcatc gcccgggctc atcattataa
     1981 tgccctcatc tatcatcctt ctgtccaatc cataagggcc atgcttctca cggctctgga
     2041 gaacgccctg tccaggatga atgaggatga tgccaaaagg aggatagagc tattcttccg
     2101 ggagtacacc gatccggacc ttctgcggtt catctgggag aagggagatg atgtctcacg
     2161 ggatctcctg cagcgtataa aattcggcca ggagataccg ctggctgcca ggttcgacca
     2221 ccggtccctc gcgccggata tcagaatggc cctgtccacc atctaccgcc atgggaggat
     2281 gagaaagctc ttcgaggata ccctctgcaa aaaatatggg gccctggtga gcatcaccgc
     2341 gggcagcgga gtgcccaggt ccatgagaac ccaggcggat agctttctct atgacgaatc
     2401 cgccctggcc gcgggcctgg taaagtcgct gacccgccag atcgccatat ccttcttctc
     2461 cgacgataga ccggagatct cccttgatga agttatggag caagcggcaa agctgctgtc
     2521 ctttattcgc tccgagagct atcttcccat cgatggacta ttgctgctct tttatgcttt
     2581 gcataatatg ctctcagaga gatacggtga gaagatcctg gttccccgga tcagaaacat
     2641 aacctggctc tacaggactg tggccaggct cagggacctg ggccctggtg ggctttcagg
     2701 actatttgat tattcctttc attctgaata cggattcccc tatagcgaga ggcttttcga
     2761 ggatattcag atcctcgttg ccaatggcat gatctatcag gaccaaagac actat
//
LOCUS       DAOSGP010000176         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_34156_length_2812_cov_21.669206, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000176 DAOSGP010000000
VERSION     DAOSGP010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34156_length_2812_cov_21.669206"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PLV49_05555"
     CDS             <1..1151
                     /locus_tag="PLV49_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG98346.1"
                     /translation="DGRTEVNQEDVKKAMELALAHRMRSRPFEPPTLNKERLEKSMSQ
                     KQHEHQHQDQKPEQQKKNEQQPQEPSEQKDTDQKQAAKPQERIFEIGTPIDVRAITMP
                     RKRDRIARRKTSGRRMNTLALRNRGRYLRQKMPQEGKDIAIDATIRAAAPYQRIRSGP
                     NAIIVKGEDIREKERVGKTSAVVLFVVDASGSMGANQRMESAKGAVLSLLMDSYQKRD
                     KIGMIAFKGKEAEIILPPCTSVDLALGRLRELPTGGKTPLSAGLSRGLQLLQGELRKD
                     EESKLMMVLISDGRANEGMGGKIKDELMAISERIKHLGIHTIVIDSEVVDSSFLDMRL
                     GYCHQIADQCQGKYYPLSSLTPESLLRIVDEEQKLLFDDAAINEYAAT"
     gene            1253..2104
                     /locus_tag="PLV49_05560"
     CDS             1253..2104
                     /locus_tag="PLV49_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG98347.1"
                     /translation="MIEISGLSKEYSRKKVVDNLNLEVGQGELFGFLGPNGAGKTTTI
                     RILTTLTKPSSGRVFINGIDVAKDPSRVKSEFGVVQQHLSLNRDLTIGENLELHARLH
                     HLQSRERKERIAELLDYVGLSDHSDQLIDSVSGGMKRRAMIARALIHRPKLLFLDEPT
                     VGLDAQTRRRVWDLIRRMNLEGSTVFLTTHYIEEAEALCQRVGILHHGRLIALGSPLE
                     LRQRLGMVAVEMQGEACGTTYRYFPDRSSASLFIQDLPGSERVVMRESNLEDVFIELT
                     GEKVMGD"
     gene            2106..>2812
                     /locus_tag="PLV49_05565"
     CDS             2106..>2812
                     /locus_tag="PLV49_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98348.1"
                     /translation="MIDAFSILWSDLTALKRRWPRYLLTTLMSPLLYLVAFGWGLGRG
                     IDMNGTSYLDFVIPGIIALTAMTTSFNGAGTRLNVDRLYFKSFDECLMAPVELPSILL
                     GKALIGVVRGILSSVAFLLVAFLIARHIHITLPFVLGLLLSCLTFAFLGVLAALLARS
                     HEDMATFGSLILMPMTFLGGTFFSVSQVPFGLKMALYALPLTHSSLFLRAAALDQPLP
                     WSSFLVLFLFFLLFLGGG"
BASE COUNT          678 a          734 c          790 g          610 t
ORIGIN      
        1 tcgatggtcg aaccgaagtc aaccaggagg atgtcaagaa agccatggag ctggccctgg
       61 ctcataggat gaggagcagg ccatttgagc cgcccacgct gaacaaggaa cggctggaga
      121 agtccatgtc tcagaagcag catgaacacc agcatcagga tcaaaagccc gagcagcaga
      181 agaagaatga gcaacaacca caggagccgt cggaacaaaa ggacacagat caaaaacagg
      241 ccgctaagcc ccaagagagg atattcgaga tcggcacgcc tatcgatgtg cgtgccataa
      301 cgatgccccg caagagggac cgaatcgccc gccgcaagac cagcggcagg aggatgaata
      361 cactggctct gcgcaaccgc ggccggtatc tgcgccagaa aatgccccag gaaggaaaag
      421 atattgccat tgatgccacc attcgtgccg ctgcccccta tcaaaggatc agatccggcc
      481 ccaacgccat catcgtgaag ggcgaggata tacgggagaa ggagagagta ggcaagacct
      541 ccgccgtagt cctcttcgtg gtagatgcca gtggatccat gggggccaat cagaggatgg
      601 agagcgccaa gggtgcagtt ctctccctgc taatggactc ctaccagaag agagacaaga
      661 tcggcatgat agccttcaaa ggaaaggaag ccgagatcat acttcccccc tgcaccagcg
      721 tggatctggc ccttggccgc ttgcgggagc ttcccaccgg gggcaagaca cctctatccg
      781 ccggcctctc ccgcggattg cagctcttgc agggagaact gagaaaggat gaggagagca
      841 aattgatgat ggttctcata tccgatggca gggctaatga gggaatgggt ggaaagatca
      901 aagacgagct gatggcgatc tcagaacgga taaagcacct gggaatccat accatcgtca
      961 tcgacagtga ggtcgtagac agctccttcc tggatatgcg tttaggctat tgccatcaga
     1021 tcgcggatca gtgtcaggga aagtactatc ccctatccag cttaaccccg gagtccctgc
     1081 tcaggattgt agatgaagag cagaagcttc tgttcgatga cgcggccata aatgaatatg
     1141 ctgcaacata atgctattat agatatgata atagaaataa agagttaata aatgtaatta
     1201 acatatataa ttaatatatg cagttaatat aattgagata gatatgagca aaatgatcga
     1261 gatatcgggc ctgagcaagg agtactcgag gaagaaggta gtagataact tgaatcttga
     1321 ggtaggacag ggagaactct tcggcttctt ggggccaaat ggagctggca agaccaccac
     1381 cattcgcatc cttaccaccc tcaccaagcc ctcgtccgga cgggttttta tcaacggcat
     1441 cgatgtggca aaggacccgt ccagggtgaa gtcggagttt ggcgtggttc agcagcactt
     1501 gagcctgaac cgggatctga ccatcggcga gaacctggag cttcatgccc ggctgcacca
     1561 cctccaatcc agggaacgaa aggagaggat cgcggaactg ctcgattatg tggggctgtc
     1621 cgatcactcc gatcagctca tcgatagcgt ctctggggga atgaagagga gggccatgat
     1681 agcccgggcg ctcatccaca ggcccaagct cctcttcctg gatgagccga cggtgggatt
     1741 ggacgctcaa accagacgcc gggtctggga tctcatccgc aggatgaacc tagaaggctc
     1801 gacggtcttc ttgaccaccc actatatcga agaggccgag gccttgtgtc agcgggttgg
     1861 aattcttcat cacggcaggc tcatagctct gggaagccct ctggagctgc ggcaaaggtt
     1921 aggaatggtg gccgtagaga tgcaggggga ggcgtgcggc acaacttacc gctactttcc
     1981 ggatcgatca agtgcctccc tgtttattca ggatcttcca ggctcggaga gggtggtcat
     2041 gcgagagtcc aacctggagg atgtgttcat cgagctgacc ggtgagaagg tgatgggaga
     2101 ctagcatgat cgatgccttc agcatactct ggtcggacct gacagcgctc aagcggcgct
     2161 ggcctcgcta tcttctcacc accctgatga gcccgcttct ctatctggtg gcctttggct
     2221 ggggcctggg tcgcgggata gatatgaacg gaaccagcta tcttgatttc gtgataccgg
     2281 gaatcatcgc tctgaccgcc atgaccacca gcttcaatgg agccggcacc aggctcaatg
     2341 tcgatcgcct ctatttcaag agctttgatg aatgcctcat ggccccggtg gaactgccct
     2401 ccattctctt gggaaaagcg ctgattgggg tggtgagggg tatactgagc tcggtagcat
     2461 tcttgctcgt agcctttctc atcgccaggc atatccatat cactctgccc tttgtattag
     2521 gattgctgct tagctgcctg acctttgcct ttcttggtgt tctggctgca ctgctggccc
     2581 gctctcatga ggatatggcc acatttggca gcctgattct catgccgatg accttcctgg
     2641 gcggaacctt cttctcagtc tcccaggttc ccttcgggct gaagatggcg ctgtacgccc
     2701 ttcctctcac ccactccagc ctgtttctga gggcggctgc attggaccag cctcttccct
     2761 ggtcatcttt tctggttctt ttcctcttct ttctgttatt cttgggcgga gg
//
LOCUS       DAOSGP010000177         2805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_34282_length_2805_cov_20.841818, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000177 DAOSGP010000000
VERSION     DAOSGP010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34282_length_2805_cov_20.841818"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..256)
                     /locus_tag="PLV49_05570"
     tRNA            complement(join(167..201,220..256))
                     /locus_tag="PLV49_05570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Arg,seq:gcg)
     gene            complement(294..1073)
                     /gene="uppS"
                     /locus_tag="PLV49_05575"
     CDS             complement(294..1073)
                     /gene="uppS"
                     /locus_tag="PLV49_05575"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOG98349.1"
                     /translation="MSQIFRNPVYLLYERLLKAQVLAGSAVEHIAIIQDGNRRYARQK
                     GLAKSLGHSLGAETSERVSDWCLEVGVRHLTLYAFSTENFSREDSEKRYIFDLIKKKF
                     NDLRNSKKIHFNKVRVRAIGRLEMLPDDLQEEIRLTEEATKDYKKMYLNVALAYGGQR
                     ELVDAVRSLARLVEEGRIRAKDLNETLIAQHLYPQDGIAVPKVDLIIRTAGDFRTSNF
                     LPWQANGNECAAYFCAPFWPEFRKIDFLRAIRTAQTRASCQ"
     gene            1196..1269
                     /locus_tag="PLV49_05580"
     tRNA            1196..1269
                     /locus_tag="PLV49_05580"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1230..1232,aa:Val,seq:cac)
     gene            1449..1769
                     /gene="eif1A"
                     /locus_tag="PLV49_05585"
     CDS             1449..1769
                     /gene="eif1A"
                     /locus_tag="PLV49_05585"
                     /inference="COORDINATES: protein motif:HMM:NF003085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HOG98350.1"
                     /translation="MYNRGHGGEEGAVITRVRLPRKQDREIFGHVESLLGSNRIKVRG
                     IDGVTRMARIPGKMKKRIWIREGDVVIIIPWEFQNEKADVVWRYTGPQVDWLQRKGFL
                     KGSS"
     gene            1786..2586
                     /locus_tag="PLV49_05590"
     CDS             1786..2586
                     /locus_tag="PLV49_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HOG98351.1"
                     /translation="MSRRSKDEDFDTRIDVLRTRIKDSDDLNVQDAVFDKRTLMDLYS
                     LASKGVIDALGGSICTGKEANIFRALVGSRELALKIYRISTSNFNVMQDYLHGDPRFG
                     SVKGTKRAIVAAWTRKEYRNLMRAEEVGVRVPHPITMKENILVMDLIGKGDDVAPPLK
                     DVGLDSAEAEQIYQKIVEYISLLYNQAGLVHADLSEFNILYDDGEPVIIDMGQSVTLD
                     HPMARKFLERDISNIVHYFQKKYSIGSSEEIYARIQKDGAEKAKKESS"
     gene            2609..>2805
                     /locus_tag="PLV49_05595"
     CDS             2609..>2805
                     /locus_tag="PLV49_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOG98352.1"
                     /translation="MDIKIPEERIGVLVGPNGSMKHFIEEKTKTSLEIDSDTGTISVT
                     SAEDPLQVLRVMDLVKAIGRG"
BASE COUNT          735 a          721 c          718 g          631 t
ORIGIN      
        1 cggctaccgt cacctccccc aggcctcgaa cgctctttgc cgccgcctcg agaccagagg
       61 cataaacgcc atcatcgttg ctgaccagta tgcgatgcat cgcatagccc tgagcatttc
      121 tcggtattaa actataccct acctgcaaac cctaaaatgc aatagacgtc ccgaccggga
      181 ttcgaacccg ggttgaaggc tctctgcgcg aaaaatgctc cgcaggcctc cgggatatcc
      241 actaccccat cgggacaaga acacctctga tggcatgctt caggttatta agcttattgg
      301 catgatgccc tggtctgtgc cgtcctgata gcccgcagaa agtcgatctt cctgaactcc
      361 ggccaaaaag gagcgcaaaa gtaggcggcg cactcattgc cattggcctg ccagggaagg
      421 aagttcgatg tacgaaaatc ccccgcggtc ctgatgatca gatccacctt gggaacagca
      481 atgccatcct gaggataaag atgctgggcg atgagagtct cattaaggtc cttggctctt
      541 atccgtccct cctccaccag gcgggctaag gagcgtacag catccaccag ctccctctga
      601 cctccgtaag ccagggccac attgagatac atcttcttat aatccttcgt ggcctcctca
      661 gtcagtcgga tctcctcctg cagatcatcg ggaagcatct ccaaccgacc gatggctcgc
      721 acccgaactt tattgaaatg gattttcttt gaatttctca gatcattgaa ctttttcttt
      781 atcaaatcaa agatatagcg cttctccgaa tcctcccggc tgaagttctc cgtggaaaaa
      841 gcataaaggg tgagatgccg aacccccacc tccaggcacc agtctgacac cctctccgag
      901 gtctcagcgc caaggctatg gccaagagat ttagcgaggc ccttctgcct ggcatacctg
      961 cggtttccat cctgaattat cgctatatgc tccaccgctg accccgccag gacctgagcc
     1021 ttgagcagcc tctcatacaa aagataaaca ggatttctga agatttgact catttactta
     1081 attaagctcg cgaatagata tttaaataat tccttgccac gagcccaaaa ctggctccgg
     1141 gatttttcct ctagggcaag atactaatat ctgatgcctt acttccttac ttcgagggcc
     1201 tgtggtctag ctggttatga catcgccttc acacggcggg gatcacgcgt tcgaatcgcg
     1261 tcgggcccat taggattcgg atcgacacaa gctttaacta tttaagcgtc attttcgact
     1321 gaggtggtgc ggcaagttgc tctggcgatc ctggctgcaa atgggccaga tagcgattcc
     1381 acaccgctca gggtctcatc gatccggcat ctaatctaaa atctgcaatt attatttgag
     1441 gtgataagct gtacaataga ggtcatggtg gggaggaagg agcagtaata accagagttc
     1501 gcctccctcg caaacaggat agagagatat tcggtcatgt agaaagcctg cttggctcca
     1561 atcgcatcaa ggtcaggggc attgacggcg taaccaggat ggcccgcata ccgggaaaga
     1621 tgaagaagag gatctggata cgagagggcg acgtggtcat aatcatcccc tgggagttcc
     1681 agaatgagaa ggcggacgtg gtctggagat acaccggccc tcaagtggac tggctgcagc
     1741 gcaaaggttt tctgaagggc tcttcataag ccggatagac aaatcatgag caggcgcagc
     1801 aaggatgagg attttgatac ccggattgat gttcttcgta ccagaatcaa ggactccgat
     1861 gacctgaatg tccaggacgc tgtctttgac aagcgaaccc tgatggacct gtacagcctg
     1921 gcaagcaaag gggtcataga tgccctgggt ggttccatct gcaccggaaa agaggcgaat
     1981 atattccggg ctctggtggg cagcagagag cttgccctca agatctatcg catttctacc
     2041 agcaacttca atgtgatgca ggattacctg catggcgatc ccagatttgg aagcgtcaag
     2101 ggcacgaaaa gagctattgt cgccgcctgg acccgcaagg agtatcgcaa cctcatgcga
     2161 gcggaggagg tgggtgtgcg ggtgccccat cccatcacca tgaaggagaa tattctggtt
     2221 atggatctga tcggcaaggg agacgatgtc gcaccacccc taaaggatgt cgggctggac
     2281 tctgcagagg ccgagcaaat ctaccagaag atcgtcgaat atatatccct gctttacaat
     2341 caagccggtc tggttcatgc cgacctgagc gagtttaata tcctatacga tgatggcgag
     2401 ccggtgataa tcgatatggg acaatcggtc accctggacc atcccatggc cagaaagttc
     2461 ctggagcgag acatctccaa tatagttcat tacttccaga aaaagtactc tattggctca
     2521 tccgaggaga tctatgccag gatccagaag gatggagctg aaaaggcaaa aaaggagagt
     2581 agctgatcaa gataaaagga ggctgacaat ggatataaag atcccagaag aacgtatagg
     2641 agtgttggtg ggacctaacg gctccatgaa acacttcata gaagagaaga caaagacctc
     2701 tctggagata gacagcgata caggcacaat ttctgtcaca tccgcagaag atccattgca
     2761 ggtattgagg gtcatggacc tggtgaaggc gattggcaga ggatt
//
LOCUS       DAOSGP010000178         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_34400_length_2798_cov_12.533358, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000178 DAOSGP010000000
VERSION     DAOSGP010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34400_length_2798_cov_12.533358"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..164
                     /locus_tag="PLV49_05600"
     CDS             15..164
                     /locus_tag="PLV49_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG98353.1"
                     /translation="MRSGHHCCIPLMRHLGLKHGTARASLYLYNTDEEIEKLLETLEQ
                     IARMA"
     gene            181..936
                     /gene="fdhD"
                     /locus_tag="PLV49_05605"
     CDS             181..936
                     /gene="fdhD"
                     /locus_tag="PLV49_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOG98354.1"
                     /translation="MIRDFECLRVDGDDSYKATCPVAEEVPLSIFINGRHFATAMISP
                     QMRKEFVAGHLYSERIVSAIEEIESIEVEGDVARAIVANPIRAIVPRRPIVSGCGGIA
                     SFLDESKLPRIKSDLTINKKQAQEAMKAISLSETHIATGGVHSVGLFDQSGPVSIIED
                     IGRHNALDKAIGSLILKGEGFNLGRAFAACTGRVSSDMALKCSVAGIPIVVSRGATTG
                     LAITIAQRTGLGIIGFLRGKRLSVYANPERFVI"
     gene            989..1897
                     /locus_tag="PLV49_05610"
     CDS             989..1897
                     /locus_tag="PLV49_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HOG98355.1"
                     /translation="MICSLCQERAIVSQRYLGQHLCQDHFIQDFEGRMVQTIEMNRMI
                     EKGERIAVAVSGGKDSTALLFGLSRVLAEKEVEMLAVTVDEGIAGYRDDTILAAKRIA
                     RQLGVEQVIVSFREKYGWSLDEMVVGAEVAPCTYCGVFRKNALNCAAKSLGATKLATG
                     HNQDDEAQSVMMNYLKGDMERLMRFRPRREQPGLIPRIKPLRDIPEKEIALYAMVNGI
                     YFESRECPYARLSLRADVRDMMNRMESLFPGTRQKTLQGFERIEELCRGDWAQMELAE
                     CRECGEPCVGKRCKACELLGMMKTIN"
     gene            complement(1939..2298)
                     /locus_tag="PLV49_05615"
     CDS             complement(1939..2298)
                     /locus_tag="PLV49_05615"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOG98356.1"
                     /translation="MALMRLLADENVPIKAIELLRKRGYDVLSISESTPQACDRDIMM
                     LAEREGRILLTFDKDFGELAFNSITKPSCGIILFRIPLRSADYIASFIAGIIESGCDW
                     PGNFAVVEPGRIRLRKL"
     gene            complement(2273..2533)
                     /locus_tag="PLV49_05620"
     CDS             complement(2273..2533)
                     /locus_tag="PLV49_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOG98357.1"
                     /translation="MNWQDRISQDPRILAGKPVIKGTRIAVEFVIELMANDWTESEII
                     QNYPRLTHEDIQACLQYASDLMKSERVYPHKVQQDGLNAATS"
BASE COUNT          650 a          696 c          806 g          646 t
ORIGIN      
        1 cctccaacat ctgcattcgc tcggggcacc attgctgcat ccccctgatg aggcatctgg
       61 ggcttaagca tggcaccgcc cgggcatcgc tgtatcttta taataccgat gaggagatcg
      121 agaagctcct ggagacttta gagcagatcg ccaggatggc ttaagggaac gcatgagaag
      181 atgataagag actttgagtg cctgagggtg gatggagatg actcctataa ggccacctgc
      241 ccggtggctg aggaggttcc cttatctatt ttcatcaacg gccgccattt tgccaccgct
      301 atgatcagcc cccagatgag aaaagagttc gtggccgggc acctttattc cgagaggatc
      361 gtctctgcca tcgaggagat cgaatctata gaggtggagg gagatgtggc cagggcgata
      421 gtcgccaatc ccattcgggc gatagtcccc aggaggccca ttgtgagcgg ctgcgggggc
      481 atagcctcat ttctcgatga gtcaaagctc ccccggataa aatccgatct gacgatcaat
      541 aaaaaacaag ctcaggaggc gatgaaggcc atatccctct ccgagacgca catcgctacg
      601 ggcggggttc actcagttgg gctcttcgat caatccgggc ctgtatcgat catcgaggac
      661 ataggaaggc acaatgccct ggacaaggcc ataggcagcc tgattctcaa aggggaggga
      721 ttcaacctgg gccgggcgtt tgccgcctgc accggccggg tctcttccga catggccctg
      781 aagtgctctg tagccggcat ccccatagtc gtctcccggg gggcgacgac gggactggcc
      841 ataaccattg ctcagaggac gggtcttggc atcatcggtt ttctgcgggg gaagaggctg
      901 tccgtttatg ccaatccgga gaggtttgtg atttgaggtt gactgaatga ggttgactga
      961 atgaggttga ctgcatgagg tcgcctaaat gatctgctcc ctctgtcagg aacgggcgat
     1021 cgtttcccag cgctacctcg gccagcacct ctgccaggat catttcatcc aggattttga
     1081 gggaaggatg gtccagacca tcgaaatgaa caggatgatc gagaagggag agaggatcgc
     1141 agtggcggtg agcggaggga aggacagcac tgcccttcta tttggcttaa gccgggttct
     1201 cgccgaaaaa gaggtggaaa tgttagcagt gacggtggat gaaggcatcg ccggctaccg
     1261 ggatgacacc atccttgccg caaagaggat cgccaggcag ctgggagtgg agcaggtgat
     1321 cgtctccttc cgggaaaagt acggctggag cctggacgag atggtggtgg gagcagaggt
     1381 tgcgccctgc acctactgcg gggtctttcg caagaacgca ttgaactgcg ctgccaaaag
     1441 cctgggggcg acaaagctcg ccaccggcca caaccaggac gatgaggccc agtcggtgat
     1501 gatgaactat ctgaaggggg atatggagcg tctgatgcgc tttcggccca ggagggagca
     1561 gccggggctg attcccagga tcaagcccct gcgagacatt cctgaaaagg agatcgccct
     1621 ctatgccatg gttaacggga tctatttcga gtccagggag tgcccctatg cccggctctc
     1681 tttaagagcg gatgtgcggg atatgatgaa ccggatggag agcctctttc ccggaacccg
     1741 gcagaagacg ctgcaggggt tcgagaggat cgaggagctg tgccgaggag actgggcgca
     1801 gatggagctg gcagagtgca gggaatgcgg cgagccatgc gtggggaaga ggtgcaaggc
     1861 ctgcgagctc ttggggatga tgaagactat caactagatg ggcattgatt ttgtaggtta
     1921 tctggtaatg cagaaggtct atagcttcct caagcgaatc ctgccaggtt ctacaaccgc
     1981 gaaattgccg ggccaatcac atccagattc tattattcca gcaatgaatg acgcaatata
     2041 gtcagccgat ctgagaggta tgcgaaacag tatgattccg caagatggct tggttataga
     2101 attgaaagca agctctccaa aatccttgtc aaaggtgaga aggatacgtc cttctctttc
     2161 cgcaagcatc atgatgtctc tgtcgcaagc ctgaggggtg ctctcactga tcgatagcac
     2221 atcgtatccc ctcttgcgca gcagctcaat ggctttgatg ggaacattct cgtcagctag
     2281 tagccgcatt aaggccatcc tgctggactt tgtgaggata taccctctcg gatttcatca
     2341 ggtcgcttgc atattggagg catgcctgaa tgtcttcatg agtcaatctg ggatagttct
     2401 ggattatctc ggattcagtc caatcattgg ccatcagctc gatgacaaat tccactgcta
     2461 tgcgcgtacc tttgatcacc ggttttccag caaggattct gggatcctgg gatattcgat
     2521 cttgccagtt catgatagaa ttataagatg tggaccctta agatatcact ttttcggctc
     2581 taatgcctgc cagcttctat tgcgaaatat ccttatctat tgggatttct ttttaatcgt
     2641 taggtcaaat aaaattggat tatgaactct gcttgttcaa tataatggtg acgccctcac
     2701 tttcgagatc tcttaaacct tcaccctcag catcctctcg attgcccctc tcgcccgcac
     2761 agcgatctcc tctggcacaa caacccgcgg ctcgagcg
//
LOCUS       DAOSGP010000179         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_34745_length_2778_cov_14.821888, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000179 DAOSGP010000000
VERSION     DAOSGP010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34745_length_2778_cov_14.821888"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1051
                     /locus_tag="PLV49_05625"
     CDS             98..1051
                     /locus_tag="PLV49_05625"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOG98358.1"
                     /translation="MSEKMTNNLKTADQQRAELFLEEFVSGLVGSMGDDMDFILLFGS
                     AARGEFILGKSDVDLIIQTKSDAAVRRVERFAESLFWRLDEKHGMHFEKVLSTGVSES
                     ILEESIKFLEKHVRLYKPFEVFGPNDIDWNEGLIKRPDLLPGAVLVASQLTLLYKMKY
                     EGKILFGRDVRPEINPRFTCWERLKAIMVPQSIAFASFVLALILPQKATGYAVKALFY
                     EVDSVNIYQKSVIPPHQEKMRSFAEASQFENAIFDRLRFLLEKNLGLSSEQKLELLRK
                     AAAIKRDGFHGSRWEALRFCVVAFGAIWQTNAALILRARGS"
     gene            complement(1046..>2778)
                     /locus_tag="PLV49_05630"
     CDS             complement(1046..>2778)
                     /locus_tag="PLV49_05630"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HOG98359.1"
                     /translation="AAPGGESMIADLTMIHAIQGDGQSSPFQGMKVSIEAIVTADFQG
                     TDKLEGFFVQEEDSDIDDNIETSEGIYIYDPGRLGVKENVTRGDLVIARGHIEEFHGL
                     TQMNLEEIKKKKAGKNENKSTLTAHQLILPLKEDSLERYEGMLLMLPQDLVITDIENF
                     SAYGELAISPQSRLPVPTNVAKPGAPAAQVQELNSRSMIILDDGSGRRFPQSYPFPRT
                     IRCADTIQGIMGIMSYGYGNYRLEPLNISKIMISNPRPTNPESVGGRLRIASLNLENY
                     FNGNGAGRGFPSARGARSYAEFELQRAKIIQAITDMKADVIGLIEIENDGYGNLSAIH
                     DLCDGLNAREDGIGLENYSFVDPGSPKLGDDLISVGLIYNRTTIRPIGRAITTSLGAF
                     SSGNRQPLAQTFEEISSLERFTIVVVHLKSKNPPGRDEVADGDNRDCGDGQGYWNGDR
                     TRAANELTKWLSSDPTGSHDPDYLILGDMNSYRNEDPIMALRRAGYVNQISSQLASNS
                     YSYVFQGRWGELDHILASPSLTGQVTGASIWHINSDESPDFGYDGIWSSPDKYRCSDH
                     DPLIAGISLS"
BASE COUNT          661 a          695 c          686 g          736 t
ORIGIN      
        1 tttaaatgaa atttctgaaa tgctcgagcg ataatggacc aatttagctg atatgacaag
       61 gcttttttcc ctgaagaaca aattaagata aataataatg agtgaaaaga tgacaaataa
      121 cctgaagaca gcggatcagc agagagctga attattcctg gaggagttcg tctctggcct
      181 tgtgggatcg atgggggatg atatggattt tatccttctc ttcggaagtg cagcccgagg
      241 cgagttcatc ctgggaaaga gcgatgtaga tctcatcatt cagacaaaga gcgatgcagc
      301 tgtccgcagg gtggaaaggt ttgccgaatc tctcttctgg cggctggatg agaagcacgg
      361 catgcacttt gagaaggttc tatcaaccgg cgtgagtgaa agcatcctgg aagaatcgat
      421 caagtttctg gaaaagcatg tcaggctcta caagcctttt gaggtatttg gaccgaatga
      481 tatcgattgg aatgaaggat tgatcaagag gccagacctt ttgccgggtg ctgttctcgt
      541 cgccagccag ctcactttgc tgtacaaaat gaaatatgag ggcaagattc tcttcggccg
      601 ggacgttcgc ccggagataa atccgcgctt cacctgctgg gaaagactga aagctataat
      661 ggtgcctcaa agcattgctt ttgcctcttt tgttctcgcc ctgatccttc cccagaaggc
      721 aactggctat gcggtcaaag ccctcttcta tgaggtggat tcggtgaaca tctaccagaa
      781 aagcgtgatt cctccccatc aggagaagat gagaagcttt gccgaagcat cgcaattcga
      841 gaatgctatc ttcgaccggt tgcgctttct tttagagaag aaccttggcc tatcaagcga
      901 gcagaagctg gagctactga ggaaagcggc tgctatcaag cgggacggct ttcacgggag
      961 cagatgggag gctttgagat tctgtgtggt cgcttttggg gccatctggc agaccaatgc
     1021 cgctttgatc ctgagagccc ggggctcatg atagactgat tcccgcgatc aatggatcgt
     1081 gatctgagca gcgatatttg tctgggctac tccaaatgcc atcgtagccg aagtcagggg
     1141 attcatcgga atttatatgc cagatgctcg ccccagttac ctgtccggtc aagcttgggc
     1201 ttgccaggat atgatccagc tccccccatc taccctgaaa cacatatgag taagaattag
     1261 aggcgagctg agaggaaatc tggttaacat atccggccct cctcaaagcc atgattggat
     1321 cctcgtttcg gtacgagttc atatccccca ggatcagata gtcgggatca tggcttccag
     1381 taggatcgga agacaaccat tttgtcagct catttgcggc ccgtgtgcgg tcaccattcc
     1441 agtatccttg accatctcca caatccctat tatctccatc cgccacctca tctctacctg
     1501 gcggattctt ggatttcaga tgaacgacca cgattgtaaa ccgctcaaga ctgctgatct
     1561 cctcgaaggt ctgggccaga ggctggcggt tgcccgatga gaaagcgccc aaggatgtcg
     1621 ttattgccct gcctatgggt ctgatcgttg ttctgttgta gatcaatccc actgatatga
     1681 ggtcatcgcc tagctttggt gatcccgggt ctacaaacga ataattctcc aacccaatcc
     1741 catcttcacg agcattcagc ccgtcacata gatcatgaat tgcgctcagg tttccatagc
     1801 catcattctc tatctcgatg agaccgatca catccgcctt catatcagtg atagcctgaa
     1861 tgatcttagc tctctgcagc tcgaactcgg catacgacct ggctcctctt gcactgggaa
     1921 aaccgcggcc tgctccattg ccgttgaagt agttttccag attcaggctg gcaatcctta
     1981 atctgccgcc cacggattcg ggattcgtcg gccggggatt ggatatcatg attttagaga
     2041 tattcaaagg ctccaatcta taatttccgt agccatagct cattatcccc ataattccct
     2101 ggatggtgtc tgcacagcga atggttcttg ggaatggata agactggggg aatcttcggc
     2161 cgctgccatc atcaagtatg atcatgctgc gactgttcaa ttcctggacc tgcgcggctg
     2221 gggctccggg cttggcaacg ttggtgggaa caggcagccg ggattgggga gatatggcca
     2281 gttccccata tgcggagaag ttctcaatat cggtgatgac cagatcttga ggaagcatca
     2341 agagcattcc ctcgtatctt tccagcgaat cctcctttag cggcaggatg agctggtggg
     2401 cggtcaaagt gctcttattc tcatttttgc cagccttttt cttttttatc tcctctaaat
     2461 tcatctgggt caggccatgg aactcttcga tatgccccct ggctatgacc agatcacctc
     2521 tggtgacatt ctctttcact cccagccgtc caggatcata gatatagatg ccctctgagg
     2581 tctcaatatt gtcatctata tcgctgtcct cttcctggac aaagaatcct tccagcttat
     2641 ctgttccctg gaaatcagcg gtaacaatag cctctatcga gactttcatc ccctgaaatg
     2701 ggcttgactg tccatctccc tggatggcat gaatcatggt cagatcagca atcattgatt
     2761 ctccgcccgg agcagcag
//
LOCUS       DAOSGP010000180         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_34809_length_2775_cov_14.442647, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000180 DAOSGP010000000
VERSION     DAOSGP010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34809_length_2775_cov_14.442647"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLV49_05635"
     CDS             <1..299
                     /locus_tag="PLV49_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOG98360.1"
                     /translation="RSLLEGDQIAARALKAGIDAEHKGILIENCRGDDVVIAPEGISG
                     NLIFRTLMLLCGAQSYGAPVLWEEEIFIDSSRARNGFDGPVMLAGAMAGMRKEK"
     gene            complement(350..784)
                     /gene="mobB"
                     /locus_tag="PLV49_05640"
     CDS             complement(350..784)
                     /gene="mobB"
                     /locus_tag="PLV49_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOG98361.1"
                     /translation="MKVIAVVGTKKTGKTTLVAALVRSLAKHGKVGTVKNMVDHPVDR
                     GDTKRHFDAGADVVIGLGEARLLVRRDRGDLESALAELEREGMDYAVVEGFKNSSLFK
                     IVMADIEVPNMVRRVRLPEVDEALVAELAEMVRGLEDYETVS"
     gene            complement(791..1984)
                     /locus_tag="PLV49_05645"
     CDS             complement(791..1984)
                     /locus_tag="PLV49_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HOG98362.1"
                     /translation="MDLLKNIISFRTVAPPGDCYQEIVEWLEPIFRDMGFETTKMKMP
                     QEVFAAKCCDSRLVGDRYNLRADLSVGAEKTLVIYAHLDVVPAEGEWDTDPFCAVQKN
                     GRVYGRGVSDCKGSVAALVAALKALLSKEKPKYNLSILLTTDEEVGGYSGLCYLTDLG
                     QVKGDLMLCMDGFSDDVGIGSNGIITWDVMVKGRSAHSGSSFLGVNAVERSILVMERL
                     MDLKKVVQARRSRLPASSPVKSVGIENLMPILNITMVNGGVKENIVPDRCVLRGDRRV
                     IPEERMEDAMEEIERALEPLRSEGIDFELKFYPGYPPMSVDPEHAWVNEVREAVERGM
                     GFMPQLSAAQGSLDQAYATEKTGIPTCVYGVGRQLESNIHAPNENVRIADLEGYTRFL
                     IELLQ"
     gene            complement(2081..>2775)
                     /locus_tag="PLV49_05650"
     CDS             complement(2081..>2775)
                     /locus_tag="PLV49_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG98363.1"
                     /translation="PIRVHYTCMTQYDATLNAPVAFPAENLTDTLGEVVSRAGLKQLR
                     IAETEKYAHVTYFFNGGKEEVNPGEDRVLIPSPKVATYDLQPQMSAYEVRDELLARLD
                     SGRYDLVVLNFANPDMVGHTGIYEAAVRAVEVVDGCVGEIVNRILSLGGAVLLTADHG
                     NAEKMRDSDTGQPHTAHTTNPVPFTLITSDRKSYSLREDGILADVAPTALELMHIPQP
                     EAMTGRTLRSIL"
BASE COUNT          570 a          885 c          669 g          651 t
ORIGIN      
        1 acaggagcct tttggagggc gaccagatcg ccgcccgcgc tctcaaggcg ggaatcgatg
       61 cagagcataa gggaattctg atcgagaact gccggggaga tgacgtcgtc atcgctccag
      121 aaggcatttc gggcaacctc atcttcagga cattgatgct cctgtgcgga gcccagtcct
      181 atggcgctcc ggttttgtgg gaggaggaga tctttatcga ctccagccgg gcgaggaacg
      241 gatttgacgg gccggtgatg ctggccgggg caatggccgg gatgagaaaa gagaagtagc
      301 atttacgccc tcttgctgtc ttgatttctt ttccgctcaa gtcaataatt cagctcacag
      361 tctcgtagtc ctccaaaccc cggaccatct ccgcgagctc cgccaccagg gcctcgtcca
      421 cctcagggag cctcaccctt cgcaccatgt tcggcacctc tatatccgcc atgacgatct
      481 tgaatagaga gctgttcttg aacccctcca ccaccgcata gtccatcccc tctctttcca
      541 gctccgccag ggcagactcc aggtctcctc gatcccgtct taccaaaagg cgtgcctcgc
      601 ccagacctat gaccacatca gccccagcat cgaagtgcct tttggtgtcc ccccggtcca
      661 cgggatgatc gaccatgttc ttgactgtcc ccacctttcc gtgctttgcc agggatctta
      721 ccagcgccgc taccagggtg gtctttcccg tctttttggt cccgacgacc gctatgacct
      781 tcataataga ctattgcaat agctcaatta agaatctggt atacccttcc agatcagcta
      841 tacgcacgtt ctcgtttgga gcatggatat tgctctccag ctgccggccg acgccgtaga
      901 cgcaggtggg aatccccgtc ttctctgtgg cataggcctg atctaagctt ccctgggctg
      961 ctgagagctg gggcataaat cccatccccc tctccaccgc ttccctcacc tcattcaccc
     1021 aggcatgctc cgggtcaacg ctcatgggcg gatatccggg atagaacttc agctcaaagt
     1081 cgataccctc agatctcaga ggctccagcg ccctctcgat ctcctccatc gcatcttcca
     1141 tcctctcctc cggtatcacc ctgcggtccc cccggagaac gcacctatcg ggcactatgt
     1201 tctctttgac tcctccattg accatggtga tattgaggat cggcatgagg ttctctattc
     1261 ccaccgattt caccggcgag ctggcgggaa ggcgggaccg tctggcctgt accaccttct
     1321 tcaggtccat aagcctctcc atgaccaaaa tcgacctttc taccgcattg acccccagga
     1381 agctcgatcc gctgtgggcc gaccgacctt taaccattac atcccaggtt atgatgccat
     1441 tgctcccgat ccccacgtca tcagaaaacc catccatgca gagcatcaga tcccctttca
     1501 cctggcccag atcggtcaga taacacaggc cggagtagcc tcccacctct tcatcggtgg
     1561 tcaagagaat gctcagattg tacttgggct tttccttgga caagagcgcc ttcaaagcag
     1621 cgacgagggc cgcgaccgat cccttgcagt ctgagacccc ccggccatag acccggccgt
     1681 tcttctgcac cgcacagaag ggatcggtgt cccattcgcc ctctgccggc accacatcca
     1741 ggtgggcata tatcaccaag gtcttttccg cccctacaga caaatcggcc cggaggttgt
     1801 accggtcccc caccagccgg ctatcacagc atttggctgc gaatacctcc tggggcatct
     1861 tcatcttcgt cgtctcaaat cccatatccc tgaatatggg ctccagccac tccactatct
     1921 cctgatagca gtctcctgga ggggcgaccg tcctgaaact tatgatattc ttcagcagat
     1981 ccataagata atctcgatct ggaagaatgg actcttcggg ctttgaatat tctcgcatct
     2041 ctaatccctt ctctaaacag ctaattaccc attctgcaac ctataaaatg cttcggagag
     2101 tccttccggt catggcctcg ggctggggga tatgcattag ctccagtgct gtcggtgcca
     2161 catcagccaa aattccgtcc tctcgaagcg agtagctttt ccggtcagaa gtgattaagg
     2221 tgaatggcac tggattggtg gtatgggcag tatggggctg gccggtatcg gaatccctca
     2281 tcttctctgc attgccgtgg tctgcggtga gaaggaccgc tccgcccagg ctcagtatcc
     2341 ggttaacgat ctcacccacg cagccgtcca ccacctccac cgccctcact gcagcctcat
     2401 agatgcccgt atggccgacc atatccggat tggcgaaatt gagtacgacc aaatcatacc
     2461 ggccggaatc gagacgggca aggagctcgt cccggacctc ataagcgctc atctggggct
     2521 gcaggtcata ggtagccacc tttggtgagg gtataagcac ccgatcctct cccggattga
     2581 cctcttcctt tcccccattg aaaaagtagg tgacatgggc atacttctca gtctcagcta
     2641 tcctcagctg ctttaatcct gcccggctga ccacctctcc cagagtgtca gtcaggttct
     2701 cagccggaaa ggccactggt gcgttcaggg tggcatcata ctgggtcatg caggtgtaat
     2761 ggacccggat cggtt
//
LOCUS       DAOSGP010000181         2762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_35055_length_2762_cov_17.769117, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000181 DAOSGP010000000
VERSION     DAOSGP010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35055_length_2762_cov_17.769117"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLV49_05655"
     CDS             complement(<1..518)
                     /locus_tag="PLV49_05655"
                     /EC_number="2.1.1.206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HOG98364.1"
                     /translation="MRAVVLRLGHRPERDKRITTHVGLVARAFGAEGMLLCGRDAHVE
                     ESLNDAARRWGGDFRLTPNASWKGEIVRWKESGGQVVHLTMYGSNLPDVIDEIRKSED
                     ILIVVGAEKVPAEVYQLADWNVAVGNQPHSEVAALAVFLDRLFLGRELCREFEGGLKI
                     VPSSRGKEVIYP"
     gene            632..>2762
                     /locus_tag="PLV49_05660"
     CDS             632..>2762
                     /locus_tag="PLV49_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOG98365.1"
                     /translation="MNQCERCQGRGVLTVGEKPCPNCNGKGTTESISLGEASDKDMKS
                     LIESGSAKCSVCQGSGKIPITEICPDCHGLGREYLCVVCKTKLDSKKELCERCAKKPL
                     VHILDPACDIRDISVGEIYQGKVSGLANFGAFVDLSDSVRGLAHNKYLKSRPEAGDAI
                     MVTVRNILPNGNIELEPAELKEYNTVEVEKDLPLSKASDLHSQVGKTVMIKGEVIQVK
                     QTGGPTIFTLADDSGLVFCAAFERAGVRAYADIDSDMMVRVIGEVSLRNNQVQVEIKS
                     MKRLWGGDASVVKDQIEKAIDLRAEPAQTEFLVKSEIMDRLRPAMKAVAKEIRRAVLK
                     SRPIVVRHHADADGISAAVAIEMAVLPLIAEVSGPDAEYHNYRRAPSKAPFYELEDVT
                     KDLTFALEDQSRHGQKMPLIVLMDNGSTEEDVPAMRQAQVYGLEMLVVDHHHPHKIVD
                     QFLVAHVNPAHAGGDFGLTTGMLATEIARMIHPEVENKIRHFPAVSAVGDRSEAPEAE
                     EYIRLVEDRFPRDDLKKIALALDYEAFWQRFNDGRGLINDILCLGRLDRHQKIVELLC
                     EQANAAIDEQLRASMGNVKSTKLPNGIIMNVLDVENFAHKFTFPPPGKTSGEVHDRLC
                     QKYDGKPVVTIGYGPDFAVLRSRGVRMNIPQMVKELMEEIPNGGVSGGGHLVVGSIKF
                     VGGMRKEVLAKLAEKIAACSVDEVPLKA"
BASE COUNT          623 a          831 c          796 g          512 t
ORIGIN      
        1 tccggataga tgacctcctt cccccgagag gagggcacta tcttcagccc cccctcaaac
       61 tcccggcaca gctcccggcc taaaaagagc cggtccagga aaacggccaa agccgccacc
      121 tcggaatggg gctggttgcc cacagccaca ttccagtccg ccaactgata cacctctgcc
      181 ggaaccttct ccgcccccac cacgatcagt atatcctcgc tcttgcggat ctcatcgatc
      241 acatcgggca gattcgagcc gtacatggtg aggtgaacca cctgaccacc gctttccttc
      301 cagcgaacga tctctccctt ccatgacgca ttcggggtga gacggaagtc ccctccccac
      361 cgtctggctg catcattcag gctctcctcc acatgggcat cgcgaccgca aagaagcatc
      421 ccctccgctc caaaagcccg ggcgaccagg cccacatggg tggtgatcct cttatccctc
      481 tccggtctat gccccagacg caaaaccact gctcgcacgg gccaaaagaa tatggtctcc
      541 cttatttaga gccttccagg accctgctat ctcgtgcccg aaaccgggat catcccgaag
      601 ctataaattc ctccaaaaca tcccaagagc gatgaatcag tgtgagagat gccagggccg
      661 tggagtgctc acggttggag agaagccctg ccccaactgc aatggcaagg gaacgaccga
      721 gtccatctcc ctgggagagg catcagataa ggacatgaag tctctgatcg agagcggctc
      781 ggcgaaatgc agcgtctgtc agggtagcgg caagattccg atcaccgaga tctgccctga
      841 ttgccatggt cttggccggg aatacctgtg cgtggtctgc aagacaaagc tggacagcaa
      901 aaaggagctg tgcgagcgtt gcgccaaaaa gccgctggtc cacatcctcg atccagcctg
      961 cgacatcaga gatatctccg tgggggagat ctaccagggc aaggtctccg gtctggctaa
     1021 cttcggggct tttgtcgatc tatccgattc tgtgcgcggc ctggcccaca acaaatatct
     1081 caagagccgg ccggaagctg gcgacgccat catggtgacg gtgagaaaca tcctgcccaa
     1141 tggcaacata gagcttgagc cggctgagct gaaggagtac aacaccgtcg aagtggaaaa
     1201 ggaccttcct ctatccaagg catctgatct gcacagccag gtgggcaaga cggtcatgat
     1261 caagggagag gtgatccagg tcaagcagac cggcggtccg accatcttca ccctggccga
     1321 cgacagcggc ctggtcttct gcgctgcctt cgagcgggcc ggagtgcggg cgtacgctga
     1381 tattgatagc gatatgatgg tccgggtcat cggcgaggtc agcctgcgca acaaccaggt
     1441 tcaggtggag ataaaatcca tgaaacgcct ctggggaggc gatgcctcgg tagttaaaga
     1501 ccagatagag aaggccattg accttcgggc cgagcctgcc cagacggagt ttttggtcaa
     1561 aagcgagatc atggaccggc tgcgtccggc gatgaaggcg gtggccaaag agatccgccg
     1621 cgccgtcctc aagtcccggc ccattgtggt ccgccatcat gccgacgctg atggcatttc
     1681 cgctgcagtg gccattgaga tggccgttct cccattgatt gccgaggtca gcggcccgga
     1741 tgccgagtac cacaactacc gccgcgcccc cagcaaagcc ccattttacg agctggagga
     1801 tgtgaccaag gacctgacat ttgccctgga ggatcagagc cggcacggtc agaagatgcc
     1861 tttaatcgtc ctgatggaca acggctctac cgaggaggat gttcccgcca tgcgccaggc
     1921 ccaggtctac ggcctggaga tgctggtggt cgaccatcac catccccaca agatcgtaga
     1981 ccagttcctg gtggcccatg tcaatcccgc ccacgccgga ggggacttcg gcctgaccac
     2041 gggcatgctg gccacggaga tcgcccgcat gatccatccc gaggtcgaga acaagatccg
     2101 tcattttccg gctgtatccg cggtgggcga ccgcagcgag gccccggagg ctgaagagta
     2161 catccgcctg gtcgaggacc ggttcccgag agatgacctc aaaaagatcg ccctcgcttt
     2221 agactatgag gccttctggc agcgcttcaa cgatggccgg gggctgataa acgacatcct
     2281 gtgcctggga aggcttgacc ggcaccagaa gatcgtcgag cttctctgcg agcaggccaa
     2341 tgctgccata gacgagcagc ttcgggcgag catgggcaat gtcaagagca caaagcttcc
     2401 caatggcatc atcatgaacg tcctggatgt ggagaacttc gcccataagt tcaccttccc
     2461 cccgcccggc aagacctcag gcgaggtgca cgaccgacta tgccagaaat atgatggaaa
     2521 gccggtggtc accatcggct acggcccgga tttcgcagtg ctgcgcagcc ggggagtgag
     2581 gatgaacatc cctcagatgg ttaaagagct gatggaggag atccccaatg gaggggtctc
     2641 aggaggaggt catctcgtcg ttggttccat caagttcgta ggtgggatga gaaaagaggt
     2701 cctggcgaag ctggcagaga agatcgccgc ctgctcggtg gatgaggttc ccctgaaggc
     2761 tc
//
LOCUS       DAOSGP010000182         2759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_35114_length_2759_cov_11.792530, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000182 DAOSGP010000000
VERSION     DAOSGP010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35114_length_2759_cov_11.792530"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1120)
                     /locus_tag="PLV49_05665"
     CDS             complement(212..1120)
                     /locus_tag="PLV49_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOG98366.1"
                     /translation="MLSSCDFKPVTLPDRDFFVQHYRRFPQRHSDNTFTNMVCWNYYA
                     NYRYAYVQDCVLLSSTIAGKTRYRPPIGPRNPDLLHEVLALALKSDETRPFVVLDQET
                     LEWISGIYPELQFQPERMYFDYVYLANDLAELPGKGYSTIRRQLNQFMKTCSPLVEDL
                     GTDNIEEINDFVEQWCEWKDCDSNPDLASEKDALCFALAHYRELELSGIAIRSEGKIG
                     AISLFEGLNEDTALVHFEKGLPECKGIYRAINAETARILAKRYTYINRESDMGVEGIR
                     EAKMRYHPHHMVQVHMLGREELEKVV"
     gene            complement(1114..1569)
                     /locus_tag="PLV49_05670"
     CDS             complement(1114..1569)
                     /locus_tag="PLV49_05670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG98367.1"
                     /translation="MRDKSLLDDNNCVVVEFVSCWNEEDIVELYRAGGWWRDYMDTSH
                     INDLIKGSFLFAVAVDGESGRAVGMGRVISDGVSDGYIQDLVVLADWQKMGVGKMIIE
                     KLIEGCRARKIAWVGLIAEPGKEDFYDSIGFRRMPGHVPMLLDWEARGC"
     gene            complement(1612..2445)
                     /locus_tag="PLV49_05675"
     CDS             complement(1612..2445)
                     /locus_tag="PLV49_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98368.1"
                     /translation="MKDISPKQQEEFSQNRYELLQQLEDFLDLPLAILGFAWLALLVA
                     DLVWGLTSPLESLLVTIWIIFVADFLLEFLLAPKKGFFLKANWLTIIALIVPAVRVFR
                     VLRIFRFSRGLTLTRIVSSINRSMKSLRLTMGRRGFGYIMALASIVALAGASGMYTFE
                     RDINPDIKSFGTALWYAVTAITVSTDYWPYSPEGRILAFLLGLFSLAATSYVTAALAS
                     YFIGSDAESDDTAISNKKAINDLRAEIQALRDENRVVISEIKSISEALSENRKTGSPP
                     N"
BASE COUNT          648 a          789 c          638 g          684 t
ORIGIN      
        1 gagccgccct ctctttaaag gatatgctat ggctctcgtt atgctctatc cagtgctcca
       61 ggaggtgctc tacctcatga tgggtcaggt catggccctt cggatcatgg ctatgatcat
      121 ggctgtgcaa aatcatcagc tccagacccc tgatgatcct tccctcattt gaaccttgcc
      181 ttcaatcctg aaaagcggca aatgagtttg gtcagaccac cttctccagc tcttcccggc
      241 cgagcatatg cacctggacc atatgatgag gatgatagcg catcttcgcc tcccggatcc
      301 cctccacacc catatcgctc tcccggttga tgtaggtgta tcgtttggcc aggatccttg
      361 ccgtctcggc atttatcgcc cggtagattc ctttgcattc agggagcccc ttctcgaagt
      421 ggaccagcgc tgtatcctcg ttcagcccct caaagaggga tatggccccg atctttccct
      481 ctgagcggat ggctattccc gacagctcaa gctcccgata atgggccagg gcaaagcaca
      541 gggcatcctt ctccgatgcc agatccgggt ttgaatcgca gtccttccac tcgcaccact
      601 gctctacgaa atcgtttatc tcctcgatat tatctgtccc caaatcctcg accaggggac
      661 tgcaggtctt catgaactgg ttgagctggc gcctgatggt cgagtatccc tttccaggca
      721 gctcggccag atcattcgcc agatagacat aatcaaagta catcctctcg ggctgaaact
      781 gcagctccgg atagataccg cttatccatt ccagcgtctc ctggtccagc accacaaagg
      841 gcctcgtctc atcgctcttc agggcgaggg ccaggacctc atggaggaga tccggatttc
      901 ttggcccgat gggaggacgg tagcgggtct tgccggcgat ggtgctggag aggagcacac
      961 agtcctggac ataagcatac cggtagttgg cataatagtt ccagcagacc atgttggtga
     1021 aggtgttgtc gctgtgcctc tgagggaacc ggcggtagtg ctggacgaaa aagtccctgt
     1081 ccgggagcgt caccggcttg aagtcgcaag agctcagcat cctcgggcct cccaatccag
     1141 gagcataggc acatgcccag gcattctgcg aaatccaatt gaatcataaa agtcctcctt
     1201 tcccggctcg gcgatcaggc ccacccaggc gattttcctg gctctgcacc cctctataag
     1261 cttctctatg atcatcttgc ctactcccat cttctgccag tcggccaaaa ccaccagatc
     1321 ctggatgtat ccatccgaga ccccatcgga gatcacccgg cccataccca cagccctgcc
     1381 actctctccg tcaactgcca ctgcgaaaag gaagcttccc ttaatcaggt cattgatatg
     1441 agatgtgtcc atgtagtctc tccaccagcc tccggcccgg tacagctcca ctatgtcctc
     1501 ttcattccag cagctgacga attcgaccac cacacaattg ttatcgtcta aaaggctttt
     1561 gtcccgcaat gagtctaccc ccaatcattt attgaaagcc gtaatttaaa cttaattggg
     1621 gggactgccc gtcttcctat tctcacttaa agcctcggat atagacttga tctcggaaat
     1681 aacgactcta ttttcgtccc tcagggcctg gatctcagct ctgagatcat tgatcgcctt
     1741 tttatttgag atggccgtat cgtcgctctc tgcatcacta cctataaagt aactggccaa
     1801 ggcagcagta acatagcttg ttgctgcaag ggaaaatagc cccagcagaa atgccaggat
     1861 cctgccctct ggactgtacg gccagtaatc ggtgctaaca gttatcgccg ttacagcata
     1921 ccaaagggcg gtcccaaagc tctttatatc cgggtttatg tccctctcga aggtgtacat
     1981 gccagaggcg ccggcaagag ccactatcga ggccaaagcc attatgtaac caaagcctct
     2041 ccgacccatg gtcaatctta gcgacttcat ggatctattg atggagctca cgatcctggt
     2101 caaagtcagc cctcgggaga accgaaagat gcgcaaaact cgaaaaactc tgaccgcagg
     2161 aactatcaga gctatgattg ttagccaatt agctttcagg aaaaagccct tttttggagc
     2221 tagcaggaac tcgaggagga aatccgccac gaaaatgatc cagatggtga ccaagaggct
     2281 ctccaggggt gaggttagtc cccagactag atctgctacc agaagggcaa gccaggcgaa
     2341 gcccagtatt gccagaggga gatctagaaa atcctccagt tgctgaagta gttcgtagcg
     2401 attttggctg aattcttcct gctgctttgg acttatatcc ttcaattaac aaacctcctc
     2461 aatgaagtat atgattatat ctttggttat cctatttccc tcttttagct tggaggggcg
     2521 gaaaaacaaa aaaaaacctc aatcaagaca ggcgtccatt aacgcagccc gcaacatgca
     2581 gtttatcgca ttgcgtggcg ccggatgctt ggaaaaaaaa ttgaagcttt tatgcacgct
     2641 gtttttgctc aagcgtatat gtcccctctc tctacctctc tggaggcctt cttgcgcagc
     2701 tccttggcca ttgatgaata gtatttcatc gtatccgcag tcaccgaggg cccaatctc
//
LOCUS       DAOSGP010000183         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_35703_length_2728_cov_18.586981, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000183 DAOSGP010000000
VERSION     DAOSGP010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35703_length_2728_cov_18.586981"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLV49_05680"
     CDS             <1..466
                     /locus_tag="PLV49_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98369.1"
                     /translation="KWVKMRHIALALLLSMTMVLLISGSVRGECIEIRDVTMHLDKDR
                     ATFELNYTLDTFTRLYVLALGCRHLEPDLISFLGGYKDVKLIKADENGAALQVNGAGK
                     NIDDFYLFYSCPFGSKDKPLKKGIEKLSVVYPEGKIETFYNVFSTQNVFCGE"
     gene            572..>2728
                     /gene="glgP"
                     /locus_tag="PLV49_05685"
     CDS             572..>2728
                     /gene="glgP"
                     /locus_tag="PLV49_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOG98370.1"
                     /translation="MELKQIMKGINDKFPHLPERIAGLRKLTYNLWWSWHPEGRELFK
                     LLHRQGWHLSGHNPVKMLNQMDAHMLKAASNDTHFLRHYDSVMARFEGYMEPETSWFC
                     AGSREPERCAIAYFSAEYGLHHALPFYAGGLGFLAGDYLKECSDLGIPLVGLGFMYPQ
                     GYLRQMVSSDGWQIGACEKLDRENAPIHKVLDGDGSPLSVKVPVMDPPLTLEVWMVQV
                     GRTKLYLIDTSSEANESQNRIISDRLYTGDREMRLRQEIVLGIGGARVLQALGEDYSV
                     LHLNEGHPAFAVLERIREKVEQGMNYSEASELVRNTTVFTTHTPVPAGHDAFAYPLLE
                     KYLSSYLPTLDLSMEEFLRQGLNPDRPGEFNMTAFALRNSAYRNAVSKKHGEVSRSMW
                     QPLWPHLPVDEVPIDYVTNGIHVPTWIDRRLGYDIFSRYMGRTWLEEHDESRIWELVC
                     EIPDEIIWNHHNAMKNMLITKIKERARNKWLEGGAEPGQIMASGVLLDPAALTLGFAR
                     RFASYKRPTLLLQDMDRLRRILNDPWRPVQIIFAGKAHGDDQQSKMLLQQVFNAAKDK
                     VSGGRIAFVEDYDELLAQYLVHGVDVWVNNPIPPMEASGTSGMKASVNGVPQLSVLDG
                     WWIEGYNGKNGWAFSGADDGLQRDAKDAASIYTLLEKDIVPLYYNLGEDGIPHGWVTV
                     MKEAIRMNGPNFSATRMAKEYTDKFYRQAIDAAMMER"
BASE COUNT          661 a          678 c          769 g          620 t
ORIGIN      
        1 caaatgggta aagatgcgac acatcgcctt agctctatta ttatcaatga caatggtcct
       61 attaatcagc ggcagcgttc gtggtgagtg cattgagatc agggatgtca caatgcatct
      121 ggacaaagat cgcgccacct ttgaactgaa ttatactctg gatacattca cccgccttta
      181 tgtgctcgct ttgggatgcc ggcatttgga gccggacctg atctctttcc tgggtgggta
      241 caaagatgta aagctcataa aggcagacga gaatggtgcg gctcttcagg ttaacggggc
      301 gggaaagaat atcgatgact tctacctatt ttactcctgt ccttttggct caaaggataa
      361 accgctgaaa aaggggatag agaagctatc cgtggtatat cctgagggca agattgagac
      421 attttacaat gtcttctcca cgcagaatgt cttttgtggg gagtaagaca attctatgca
      481 aataccattc tgcgggcttg tatgaccatc agatggccat atcatctgat atcgaaggct
      541 atatcattga gaataaccgc atggaaattg cttggagctg aaacagatca tgaaaggcat
      601 caacgacaaa tttccccatc tgccggagag gatcgccgga ttaagaaagc tgacctacaa
      661 cctatggtgg agctggcacc ctgagggcag ggagctattt aagctcctgc acaggcaggg
      721 ctggcatctg agcggtcaca atccagtaaa gatgctcaac cagatggatg cgcatatgct
      781 caaagcggca tcaaatgata ctcattttct gaggcattat gattctgtta tggccaggtt
      841 cgagggctat atggagcctg aaactagctg gttttgtgcc ggcagccgtg agccggagag
      901 atgcgctatt gcctacttct cagcagaata cggcctgcat catgcccttc ccttttatgc
      961 cggcggtctg ggattcctgg ccggggacta cctgaaagag tgcagtgacc tgggcatccc
     1021 tctggtagga ttgggattca tgtatcccca gggctacctc cgccagatgg tctcatcaga
     1081 cggctggcag atcggggctt gcgagaagtt ggacagagag aatgcgccca ttcacaaggt
     1141 tttggatggg gatggaagcc cactctcggt caaggttccg gtcatggacc ctcccctcac
     1201 actcgaggtc tggatggttc aggtaggaag gaccaagctc tatctgatag atacctccag
     1261 cgaggccaac gaatcgcaga accggatcat ctccgaccgg ctctacaccg gcgaccggga
     1321 gatgcgcctg cgccaggaga ttgtcctggg aatcggcggg gcaagagtat tgcaggcttt
     1381 gggcgaggat tattccgtcc ttcatctgaa cgagggccat cccgctttcg ccgtgctgga
     1441 gaggatcaga gaaaaggtcg agcaggggat gaactacagc gaagcctctg agctggtcag
     1501 aaataccaca gtgttcacta cccacacccc ggttccagcg gggcacgatg cttttgccta
     1561 tccgcttctg gaaaagtatc tctcatccta tcttccaacc ctggatcttt ctatggagga
     1621 gttcctgaga caggggctca atcctgaccg gcctggtgag ttcaatatga ccgccttcgc
     1681 tcttcgcaac tcagcctacc gtaatgctgt gagcaagaag cacggagagg tgagcaggag
     1741 catgtggcag cccctgtggc ctcatcttcc tgtggatgag gttcccatcg attatgtcac
     1801 caatgggatt catgtcccca cctggatcga ccgccggcta ggttatgaca tcttcagccg
     1861 ctatatgggc aggacctggc tggaggagca tgatgagagc cgcatctggg agctggtctg
     1921 cgagattccg gatgagatca tctggaacca ccataatgcc atgaaaaaca tgctgatcac
     1981 caagataaag gagcgagcgc ggaacaaatg gctggaaggt ggtgccgaac caggccagat
     2041 catggcctct ggagtgttgc tcgatcccgc cgccctcacc ctggggtttg ccaggcgctt
     2101 cgcctcttat aagcgaccta cccttctcct gcaggatatg gatcggctga gaaggattct
     2161 caatgatccc tggaggccgg ttcagatcat ctttgcagga aaagcgcatg gggacgacca
     2221 gcagagcaag atgctcctcc agcaggtatt caatgccgcc aaggacaaag tatccggagg
     2281 aaggatcgca ttcgtcgagg actatgatga gctgctggcc cagtatttgg tgcatggcgt
     2341 cgacgtctgg gtgaacaacc ccatccctcc catggaggca agcggaacca gcggaatgaa
     2401 ggcttcggtg aatggcgttc cccagctatc cgtcctggac ggctggtgga ttgaagggta
     2461 caatggcaaa aacggctggg cgttctctgg tgcggatgat gggctacaga gagatgccaa
     2521 ggacgcagcc tctatctata ctcttctgga gaaggatata gtgccactgt attataatct
     2581 gggcgaggat ggcattcctc atggctgggt gacagtgatg aaggaggcca tcaggatgaa
     2641 cggtccaaac ttctctgcca cccgcatggc caaggagtat actgacaagt tctatcgcca
     2701 ggccatcgat gccgccatga tggaaagg
//
LOCUS       DAOSGP010000184         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_35705_length_2728_cov_10.691732, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000184 DAOSGP010000000
VERSION     DAOSGP010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35705_length_2728_cov_10.691732"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..638
                     /gene="moaC"
                     /locus_tag="PLV49_05690"
     CDS             126..638
                     /gene="moaC"
                     /locus_tag="PLV49_05690"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOG98371.1"
                     /translation="MDSRIEDERMKEEDGWFGEVAGAEKLEGMVDITSKSLVYRKATA
                     TGSIRLKESTLQAIRCGEIKKGDPLATARLAAILAVKETPRLIPLCHPIPITGLVVGL
                     ELEVMRIRATVTVTSVGRTGVEMEALTGVSVALLNIWDMVKYLEKDETGNYPETEIEE
                     IRVLEKVKGE"
     gene            829..2487
                     /locus_tag="PLV49_05695"
     CDS             829..2487
                     /locus_tag="PLV49_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98372.1"
                     /translation="MVESRQDLIRELCTAQSFQRGLRYFEEGRVKIVEASSSRIVAKV
                     VGKDNYRVEVDLDHLSAVCNCPYDLEGYCKHIVAVFMAVDREPEKVNSMIDECIQELE
                     KMSSLLKNADPDELDDFFRRELGANAELRSRFLARFSAVGEGRSLSSYKDEIESRFEE
                     AEDEHGLIYYDNNLDFESFQNLAEIYIQKNDLLEAAKIYQALTEKISEKMDHVDDSDG
                     YYGGVFSHSLEAFVECIKNAGLDAEARRKYIDYLFQRYLLRDPDYFQDDYFDALKELC
                     SSKEDLQFWNNRLASYLPVGLADKKDDPSSHYRDKERISMRLYLLSRLNETSNFYALM
                     DENYRSSSDLCLQYAKKLLENKDRSKAIKIAEEGIALFPEYSSRYLREFLRENYRETD
                     HKKYRAQLLSLFLLTWEWKYYEQLKAAVKKEEWKETLDDILANVATKGMSRTKLIDIY
                     LKERMFDAALEEVMAQKSIHSLRAYHGKLANLYPRQYFAAYKELIHPYAESRLGREHY
                     RDVVAILKNMVGIKGFESEAEEIIERLRMENKRKPAFIDELKGL"
BASE COUNT          773 a          548 c          768 g          639 t
ORIGIN      
        1 gggattcggc atgctggcca ccgatgtggt ggagatgatc cctcaggcca tgaggtttta
       61 ggatggagct gagatagaaa gacgggaaac ggtgggcagg gattgggaag agagcagaat
      121 tgagaatgga cagtagaatt gaggacgaga ggatgaaaga agaggacggc tggtttggag
      181 aggtggccgg ggcagaaaaa ctggagggca tggtggatat cacctctaag tctctggtct
      241 acagaaaggc cactgctact ggatctataa ggctgaagga gtccactctc caggctatta
      301 gatgtggaga gataaagaag ggcgatccac tggctaccgc caggctggcg gccatactgg
      361 cagtgaagga gactcctcgt ttgattcctc tctgccaccc cattcccata accggcctgg
      421 tggttggcct tgagctggag gtgatgagga tcagagccac agtcacagtg acctctgttg
      481 gaaggacggg ggtggagatg gaggccctca caggtgtgag cgtagctctg ctcaacatct
      541 gggatatggt caagtatctg gagaaggacg agacgggcaa ctatcccgag acggaaattg
      601 aggagatccg ggtgctggag aaggtgaaag gggaatagct ctcgatgcag ggtgatatcg
      661 atatgatggt ggccaagttt catcctcgcc cgggaactgc tggtcgcggg cctaaagtgt
      721 ctgggaatac cctgcagcgt tccaggtacc gctttatata tattgcaaaa acgactgttt
      781 gattctcgta ggtcatcaat atttcatctg tgatatggga ggtcacctat ggtcgagtcc
      841 aggcaagatc tgataagaga actctgcact gcgcagtcct tccagagagg attgagatac
      901 ttcgaagagg gccgagtcaa gatcgtggaa gcttcatctt caagaattgt cgcgaaagtg
      961 gtcggaaagg acaactatcg ggtggaagta gatttagacc atctctctgc ggtctgcaat
     1021 tgtccttacg acctggaagg atactgcaag cacattgtgg ccgttttcat ggctgttgac
     1081 agagaaccag aaaaggtcaa tagcatgata gatgaatgca tccaggagct ggagaagatg
     1141 agtagtcttc tgaagaacgc agatccagat gagcttgatg atttctttcg ccgggaattg
     1201 ggggcaaatg cagagctcag gtctcgcttc ttagctcgat tctccgcagt tggggaagga
     1261 agaagcctat ccagctataa agatgagatc gaatcaagat tcgaagaggc tgaagatgag
     1321 catggtctga tctattatga caataatctg gatttcgagt cttttcagaa tcttgccgag
     1381 atctacatcc agaaaaatga tctcctggaa gctgccaaga tctaccaagc tttgacagaa
     1441 aagatatctg agaagatgga ccatgtggac gattcagacg gatattacgg aggggtattc
     1501 tctcactcac tggaggcatt tgtggaatgc ataaagaatg cagggctgga tgccgaagcc
     1561 aggagaaaat atattgacta tttatttcaa aggtatctct taagggaccc ggattacttc
     1621 caggatgatt acttcgatgc tttaaaggag ctctgcagct ctaaagagga tttgcagttc
     1681 tggaacaata ggctggcctc ctatcttcca gtggggcttg cggataagaa agatgaccca
     1741 agcagccatt accgggacaa agagcggatc tctatgcgcc tttatctgct atccaggctc
     1801 aacgaaacat ccaatttcta tgcattgatg gatgagaatt accgttccag ctcggattta
     1861 tgcctccaat atgctaagaa gcttttggaa aataaagatc gctccaaagc aataaaaata
     1921 gctgaagagg gtatagctct ttttccagaa tactcatcaa gatatctgag agaatttctg
     1981 agagaaaatt acagagagac agatcataag aagtatagag cgcaattgct gtcgctcttt
     2041 cttttaacct gggaatggaa gtactatgag caactgaagg cagcagttaa gaaagaagag
     2101 tggaaggaga cgctcgacga tattctggcc aatgtcgcga caaaaggaat gagcagaaca
     2161 aagctcattg atatctacct gaaagagcgg atgtttgatg ccgctcttga ggaggtgatg
     2221 gctcagaaga gcatccacag cttgagagct tatcatggga aattggcaaa cctctatccg
     2281 aggcaatatt tcgccgcata caaggagctg atccatcctt atgccgaatc tcgcctgggc
     2341 agagagcact atcgggatgt ggtcgcaatt ctgaagaata tggtaggaat caagggcttt
     2401 gagtccgagg cagaagagat catcgagcgg ctgcgaatgg agaataagag gaagccagct
     2461 ttcatagatg agctgaaggg attgtaatgc gataaagatc cctttgcagc ccggatgcat
     2521 tttttgattt tgccagatta tccatgcagt caagatggga gatggccaga cacatgcggg
     2581 gatttttggc cagaatatca cgatgctgca acttcagcct ccagtaatcg ccgcccaagg
     2641 cgggtccagt tcatccatag gcgcgctcat attggagtac gaattgcagt tggcacccag
     2701 cccggatcgt ccacaaaaga agctattt
//
LOCUS       DAOSGP010000185         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_35900_length_2717_cov_17.690834, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000185 DAOSGP010000000
VERSION     DAOSGP010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35900_length_2717_cov_17.690834"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..820)
                     /locus_tag="PLV49_05700"
     CDS             complement(83..820)
                     /locus_tag="PLV49_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOG98373.1"
                     /translation="MSLAKRSIAEAVGTFILVYFGAGAAAITLMISQGSSPPNPFNIG
                     IGTLGGLGDWFAIGMAFAIAIAASIYALGRISGAHLNPAVTIALLATRRFSLGDAIPY
                     ILAQLVGASVASFLFAASVGMDAVTVGGLGATAPFPGIGYWQAVIVEAIGTFLLMLTI
                     MGVAVDRQAPPGFAGLVIGLVVGGIITTIGNITGSSLNPARTFGPYLGDYILGGNSLW
                     HFFPIYIIGPVAGALIAAFLYDYLSAE"
     gene            complement(823..1032)
                     /locus_tag="PLV49_05705"
     CDS             complement(823..1032)
                     /locus_tag="PLV49_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HOG98374.1"
                     /translation="MKCYLCALAQKDTDAVAICILCGMGVCMDHLVREELEIWEGGYP
                     FPSKKMKRTLPRILCKPCYAAYHEG"
     gene            complement(1095..2594)
                     /locus_tag="PLV49_05710"
     CDS             complement(1095..2594)
                     /locus_tag="PLV49_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2193 domain-containing protein"
                     /protein_id="HOG98375.1"
                     /translation="MSELYRKMVEEAMAAQRADVETVKRKRGQNFQVEDAKAYLDVVN
                     KMKAVEGQSKSVIALHVDSVNSHYEVIKSLTKTIRPEDDPFVEHYQTPAILEILCEQD
                     ESFKKSLKAFIDGVGRSEALIGLEAARRYAGFYGPTCVVDFALIPGSTSNVVNRILKT
                     IDIPDVHKQAILAAKSWGMNTSYGIGEVFAQEAEKGTSLAEAVRKEIDEIKYIYESPI
                     EAQGKLMDNAGHQSFDVRKYMTEYKKRMMPAVRAAMNEGVHYGNILTIPAYCVGDIAH
                     HISQSTFNMCKDDVIMAVIEAVTEVMSSTLNNSLDSFKNEHQPLNLATGAAASAVEYI
                     AELDGFNAIGIVDLLTKRFHNYVQLYPTRGAAAELHNCDFMDMIYRGWKVIDKARRLK
                     NGLGGKLVPRISGFDVDLEPIHRNEVLMNPQRYAYPACAITVRFSALMRLSDYPCLLT
                     SEPVTATMMTNIIALHKEIPASPARVCKNCASAALVDFRHSYCQYREAV"
BASE COUNT          619 a          752 c          680 g          666 t
ORIGIN      
        1 gggcagggaa gagaagacag atcgaaagat tgggctatga gcatcggata gggctataag
       61 caaaaaaaat atcaaaaaaa aattattctg ccgagagata gtcgtacagg aaggcggcga
      121 tcagagctcc tgctaccggc ccgatgatgt atatggggaa gaaatgccac aggctatttc
      181 cgcccaggat gtaatcccca aggtagggcc cgaatgttct tgccggattg agagatgagc
      241 ctgtgatgtt cccgatggtg gtgattatgc cccctaccac caggccgatc accagccccg
      301 caaatcccgg cggagcttgc ctgtccacag ccactcccat gatggtcagc atgagcagaa
      361 aggtgcctat ggcctccact attaccgcct gccagtatcc gattcctgga aatggcgctg
      421 tggctcccag accacctacc gtgactgcat ccatgcccac gctggcggca aacaggaaac
      481 tggcgacgga tgcgcccacc agctgggcca ggatataagg aatggcatct cccagggaaa
      541 atctcctcgt ggccaagagg gctatcgtca ctgctgggtt cagatgggct cctgagatcc
      601 tgcccaatgc atatatagaa gctgcaatgg cgatggcaaa agccattcct atggcaaacc
      661 aatcccccag gcctcccagg gtgcctatgc ctatgttaaa tgggttgggc gggcttgatc
      721 cctgggagat catcagggtt atcgctgccg cgcctgcccc gaagtagacc aggatgaaag
      781 tcccgaccgc ctcggcaatt gacctttttg ccagtgacat tttcatccct catgatatgc
      841 agcatagcat ggcttgcaga gaatacgggg cagcgttcgc ttcatcttct tggaggggaa
      901 gggatagcct ccctcccaaa tctccagctc ctctctgacc aggtgatcca tgcagacccc
      961 cattccgcag agtatgcaga tagccaccgc atcagtatcc ttttgagcca gagcacaaag
     1021 atagcatttc atatatccac catcaacctt gattggaatt caatatgctt cttttcttgc
     1081 tgtttgatgc tgcttcatac tgcttctctg tactggcagt aggagtgcct gaagtcgacc
     1141 agtgccgcag aagcgcagtt cttgcatacc ctggcaggtg atgctggaat ctccttatgc
     1201 agggcgatga tgttggtcat catcgtagcc gtcaccggct cgctggtaag gaggcaggga
     1261 tagtcagaca gcctcatcag agcagagaag cgcacagtga tggcacaggc cggataggca
     1321 taacgctggg gattcatgag aacctcattt ctgtgaatgg gctcaagatc cacatcgaag
     1381 cccgaaatcc tggggacgag cttgccgccc aggccgttct tcagtcttct ggccttgtca
     1441 atcaccttcc atccccggta gatcatatcc atgaagtcgc agttatgcag ctcagccgct
     1501 gctcctctgg tgggatagag ctgcacatag ttgtggaacc tcttggtgag aaggtccact
     1561 atgcctatgg cgttgaagcc gtccagctct gctatatact cgactgcgga agcggcggca
     1621 ccagtggcca ggttcagagg ctggtgctcg ttcttgaaac tgtccagaga gttattaagg
     1681 gtggagctca tgacctcagt gactgcctcg atgactgcca ttatgacgtc atccttgcac
     1741 atgttgaagg tggactggga tatatgatga gcaatatccc ccacacaata agcgggaatg
     1801 gtaaggatgt tcccatagtg aaccccttca ttcatcgccg ccctgaccgc gggcatcatc
     1861 ctcttcttgt actcggtcat gtatttgcga acatcaaagg actggtggcc agcattgtcc
     1921 atgagctttc cctgagcctc aatgggactc tcgtaaatgt acttgatctc atcgatctcc
     1981 tttcttaccg cttccgccag actggttcct ttctctgctt cctgggcaaa gacctcgcct
     2041 atgccatagg aggtgttcat gccccaggat ttggcagcca gtatagcctg cttatggacg
     2101 tcaggaatat cgatcgtctt caggatcctg ttgaccacgt tacttgtgct gcctggaatc
     2161 agggcaaagt ccaccacgca ggtcggaccg taaaatcctg catatctgcg agcagcctca
     2221 agcccgatca gagcctctga tcttcccacc ccatcgatga aggccttaag gctctttttg
     2281 aagctctcgt cctgctcgca caatatttca agaatggcag gggtctggta atgctcgaca
     2341 aacggatcgt cctccggtct tatggtcttg gtcagggact ttatgacctc gtaatgagag
     2401 ttgaccgagt ccacatgcag agcgatgacc gatttggact ggccctcgac ggccttcatc
     2461 ttgttcacta catccagata ggccttggca tcctctacct gaaagttctg ccctcttttt
     2521 ctcttgaccg tctccacgtc tgcccgctgg gctgccatgg cctcttctac catcttcctg
     2581 tatagttcag acatcttcct cacccttatc aattggaggc cgcaattctc tcatgatatc
     2641 ctgagcacca tatcctgagc atcagcaata aaacattttt aaagcctcag ttctgattgg
     2701 ttgtatcctg cctcctg
//
LOCUS       DAOSGP010000186         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_36111_length_2706_cov_16.123350, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000186 DAOSGP010000000
VERSION     DAOSGP010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36111_length_2706_cov_16.123350"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..336
                     /locus_tag="PLV49_05715"
     CDS             100..336
                     /locus_tag="PLV49_05715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HOG98376.1"
                     /translation="MTKFAVKDHEMVPEHILLTPEEGLDVLKEFGIGAPQLPKIHISD
                     PAAKEIGAKVGDIIKIIRKSTTAKHSTFYRLVID"
     gene            347..1837
                     /locus_tag="PLV49_05720"
     CDS             347..1837
                     /locus_tag="PLV49_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HOG98377.1"
                     /translation="MLDRNSLYSAYFTRDKLVHHHINSFNDFIDKGLQKVIDEVNIIE
                     TNIENTYVKLGNIRVEKPMVREADGSVERLYPNDARLRNLTYASPIKLEMTIVEGETE
                     KDPVEADIGTLPIMLNSKVCNLSGLSADEMISFGEDPSDPGGYFVVNGSERVIMTLED
                     LAPNKILVEYNQRYDENIEVAKVFSQRQGYRSLVIVERGRRSILEVSFPSVSGRLNFV
                     TLLRSLGMETDMDIVKSVSDNPEIIKFMLENLEEAEVSTNDEALEKIGSRVAAGQAKE
                     YQKKRATYVLDRYLLPHIGVDEKDRYAKALFLSRMAEACFELALARRSEDDKDHYSNK
                     RLKLSGDLMEDLFRVAFNRLTRDIKYQLERASMRNRDLNVVTTVRADVLTERMIHPLA
                     TGNWVGGRTGVSQLLDRTDYMATLSHLRRVISPLSRSQPHFEARDLHATQWGRICPSE
                     TPEGPNCGLVKNFAQGVELSKGIDDSEDIKKILMDLGVISVGGSIG"
     gene            1830..>2706
                     /locus_tag="PLV49_05725"
     CDS             1830..>2706
                     /locus_tag="PLV49_05725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585953.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. The beta
                     subunit is part of the catalytic core which binds with a
                     sigma factor to produce the holoenzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HOG98378.1"
                     /translation="MAEEMTGGARVYVNGEIVGYHDDPKTLVENLRRLRHSGSISSQT
                     NVAYFEDTGEIFMNTDSGRARRPLIVVEDGRALVTDEHVTRIQKGEMKFDDLVAEGLV
                     EYLDAEEEENSLIAIWEEDITPEHTHLELDPSMILGIAAGLVPYPEHNASPRNTMGAG
                     MVKQCLGMSTANMRLRPDTRGHYLNSPQKAIVRTKTSKAIGFDERPAGQNFVVAVLAY
                     EGYNIEDALVMNRGSIQRGLARSHFFRVSEAEERKYPGGQEDKFEIPDVEVRGARGSE
                     AYDQLDSDGLVNPNAY"
BASE COUNT          662 a          730 c          788 g          526 t
ORIGIN      
        1 gcatgaagat accaggtggt cgagggttca aatccccccg agcccgtttg gagtttcctt
       61 gagcctgtat gctgctcttt ctaagagttg gtggttctag tgaccaagtt tgccgtaaaa
      121 gatcatgaga tggttccgga acatattctg ctcactccgg aagagggcct cgatgtgctg
      181 aaggagttcg gcatcggcgc gccacagcta ccaaaaatcc atataagtga ccccgcagcc
      241 aaagagatcg gggccaaagt gggcgacata atcaaaataa tacgaaaaag cacaacagcc
      301 aagcattcga ccttttaccg gctggtaatc gattaagggt gatcttttgc tcgacagaaa
      361 ttctctttat agcgcttatt ttaccaggga taagctggtg catcatcata tcaactcatt
      421 caacgatttc atagataaag gcctgcaaaa ggtgatcgac gaggtgaaca tcatcgaaac
      481 caacatcgag aacacctatg tcaagctggg gaacatccgg gtggaaaagc ccatggttcg
      541 cgaggcggac ggctccgtgg aaaggctcta ccccaacgat gcccgccttc gcaacctgac
      601 ctatgcctcg ccaataaagc tggagatgac catcgttgag ggggagacgg agaaagatcc
      661 ggtggaggcg gacataggga ccctgcccat aatgctcaac tccaaggtct gcaacctctc
      721 cggcctcagc gcagatgaga tgatatcctt tggagaggac ccctctgacc ccggtggcta
      781 cttcgtggtc aacggatcgg agagagtgat catgaccctg gaggatctgg cgccaaacaa
      841 gattctagtg gagtacaacc agcgctacga tgagaacatc gaggtggcca aggtcttcag
      901 tcagaggcag ggctacagat cgctggtcat tgtggagagg ggtcgccgat ccatcctgga
      961 ggtctcattc ccctccgtct cgggcagatt gaacttcgtc acccttctcc gctcgctggg
     1021 gatggagacg gatatggata tcgttaaatc ggtatcagat aacccggaga tcatcaagtt
     1081 catgctggag aacctggagg aagcagaggt ttccaccaac gatgaggccc tggagaagat
     1141 aggctctcgc gtcgcagccg gccaggccaa ggagtaccag aaaaagaggg cgacctatgt
     1201 cctggaccgc tacttgctcc cccacatagg cgtcgatgag aaggataggt atgcaaaagc
     1261 tctcttcctc tcccgcatgg ccgaggcctg cttcgagctg gccttagctc ggcgcagcga
     1321 ggacgacaag gaccactact ccaacaagag gctgaagctc tcaggagacc tgatggaaga
     1381 cctcttccgg gtggctttca accgtttgac ccgggatata aagtatcaac tggagagagc
     1441 tagcatgaga aacagagacc tgaatgtggt gaccaccgtc cgggcggatg tcctcaccga
     1501 gaggatgatt caccccctgg ccacaggcaa ttgggtagga gggagaaccg gcgtctccca
     1561 gctcctggac aggaccgatt atatggccac cctctcccat ctgcgcaggg tgatatctcc
     1621 tctatcccgg tcccagcccc acttcgaggc cagagatctg catgccaccc agtggggacg
     1681 catctgtccc tctgagacgc cagagggtcc aaactgcggc ctggtgaaga acttcgccca
     1741 gggcgtcgag ctctccaaag ggatagacga ttcggaagac atcaagaaga ttctcatgga
     1801 tttaggggta atttcggtag gtggtagcat tggctgagga aatgactggc ggcgctagag
     1861 tatatgtgaa cggtgagata gtgggctatc atgatgatcc caagactctg gtcgagaacc
     1921 tccgccgcct ccggcactcg gggagcatca gcagccagac gaacgtagcc tatttcgagg
     1981 ataccggtga gatcttcatg aacaccgact ctggccgggc gaggaggccc cttatcgtgg
     2041 tcgaggatgg ccgggccctg gtcaccgatg agcatgttac caggatccag aagggagaga
     2101 tgaaattcga tgacctggtg gctgagggcc tggtggagta cctggatgca gaggaggagg
     2161 agaacagcct catcgctatc tgggaggagg acatcactcc cgagcatacc catctggagc
     2221 tcgacccatc catgattctg ggaatcgctg ccggcctggt cccctatccc gagcataacg
     2281 ccagccccag gaacaccatg ggcgcgggaa tggtcaagca gtgcctgggc atgtccaccg
     2341 ccaacatgag gctccggccc gacacccgcg gccactatct caacagccct cagaaggcta
     2401 tagtgcggac gaagacctcc aaggccatcg gctttgacga gaggcctgca ggccagaact
     2461 tcgtggtggc agtcctcgct tatgagggat acaacattga ggatgccctg gtcatgaacc
     2521 ggggctcaat ccaacgtggc ctggctcgca gccacttctt ccgggtctca gaggcagagg
     2581 agcgcaagta ccccggcggc caggaggaca agttcgagat cccggatgtt gaggtccggg
     2641 gggctagagg aagcgaggcc tatgaccagc tcgattcaga cggcctggtc aaccccaatg
     2701 cctatg
//
LOCUS       DAOSGP010000187         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_36347_length_2694_cov_16.702918, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000187 DAOSGP010000000
VERSION     DAOSGP010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36347_length_2694_cov_16.702918"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLV49_05730"
     CDS             complement(<1..965)
                     /locus_tag="PLV49_05730"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HOG98379.1"
                     /translation="MILKNVICPACGACCDDIRIDLDGNRTIVKNACKIGNARFQAIA
                     GDHRIKDPMINVGGKLQKSAWNEALEKAAKILADSKRPLLFIGGETSCETMKAGLNLG
                     EYLGAVVDSIATVCHGPSAMGIQEAGRIGSTAGQSKTRADLVVYWGSNPLELVPRHMS
                     RYAVYPRGYWTGRGRFDRTVITVDPRKSITSENSDLHIPLMPNTDYELLSALLTLIHG
                     KKPHPSVEEITGVSISMMEKMLQMMKNCKYGVIYVGLGIASSYGKHRNVELAFNLVKE
                     LNAHTKFVISILRGYCNAAGFSQTASYLYGYPFGLDFARGYPRYNP"
     gene            complement(992..2050)
                     /locus_tag="PLV49_05735"
     CDS             complement(992..2050)
                     /locus_tag="PLV49_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOG98380.1"
                     /translation="MLENDLIKLTPQPFQELKEKVWQKGICAGCRGCIAVCRADTLAY
                     DHELNKPCEVNPCIDCRACLDACPRLPANVESIVSSDVIGPYLDIKNVRSVKANERFQ
                     NGGAVIALLTAALEEELVDCALVMGLDRWAQKSYPFVVYDASELNRSAGSKYTSNEVL
                     ESIKELVKDQMVKNIALVGTPCAAQSVGLLRKSSNEYAVKLVQKIRFLIGLFCFEAFS
                     DDVMAEITGRIGVPPWRIDKMSAGEGQMTVTLRDRSMKSIPLAELEKYVKPGCRFCTD
                     FTAKLSDISVGGIGSAPGMSSVVIRTPEGMGLFNIAEEMGFLRVSDGVNVEAIKKAGG
                     QKIRKNRMANELAISGQR"
     gene            2504..>2694
                     /locus_tag="PLV49_05740"
     CDS             2504..>2694
                     /locus_tag="PLV49_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98381.1"
                     /translation="MVQTGRDECPAGGVDSLLEGTELMAGLEGPKRLFERTRWRAWLH
                     IFTGRREAFRDAGKEGPSTP"
BASE COUNT          674 a          698 c          603 g          719 t
ORIGIN      
        1 ggattgtagc gtggataacc tctggcaaaa tcaaggccga agggatagcc atagaggtag
       61 gacgcggtct ggctaaatcc tgctgcgttg caatatcctc tcagaatact tattacgaat
      121 ttggtatgtg cgttgagttc cttgaccagg ttgaaggcca actcaacatt acgatgtttt
      181 ccataggatg aggctatacc aaggcctacg tagataactc catatttaca gtttttcatc
      241 atctgcaaca tcttttccat catggagatt gagacgccag tgatttcttc aacagaagga
      301 tgaggcttct taccgtgaat gagcgtcaaa agtgccgata gaagctcata gtctgtgttg
      361 ggcataagag gaatgtgcag gtcagaattc tcagatgtga tcgattttct gggatcgaca
      421 gttataaccg tcctatcaaa tcttccccgc ccagtccagt aacctcttgg ataaacagca
      481 tatctggaca tatgccttgg caccaattca agcggattgg agccccaata aacaaccaag
      541 tcggctctag ttttgctctg accggctgtt gatcctattc gcccagcctc ctgtattccc
      601 atagcgctag gcccatgaca aactgtagcg attgaatcaa caacagcgcc caagtattct
      661 ccgagattca aacctgcttt cattgtctcg caggaggttt ctccgcctat gaacaggaga
      721 ggtcgctttg aatctgccaa aatctttgcc gctttttcta gagcctcatt ccatgcggat
      781 ttttgcagct tgccgccaac atttatcatg ggatctttaa tccgatgatc gcctgcaatc
      841 gcttgaaacc tggcattgcc aatcttgcat gcatttttga caatcgtcct atttccgtcc
      901 aggtctattc gtatgtcgtc gcagcaagcg ccgcaggctg gacagataac attctttaag
      961 atcataatct cccaaaaaaa taatctccga gctatctttg tcctgaaatt gccaactcgt
     1021 tcgccatacg gttcttcctg atcttttgcc ctcctgcttt cttgatagcc tcaacattaa
     1081 cgccatcaga gactctgagg aagcccattt cttcagcaat attgaataag cccatgcctt
     1141 cgggcgttct gataactaca gaactcattc cgggagcgct tcctatgccg cccacggaga
     1201 tatctgaaag cttggctgta aagtcggtgc aaaatctgca gccgggcttg acatattttt
     1261 ctagttcggc caggggaatt gacttcatgc ttcgatcccg aagcgttaca gtcatctgtc
     1321 cctcacctgc gctcatttta tctattcgcc aaggtggaac gccaatgcgc cctgttatct
     1381 ctgccatcac atcatcactg aatgcttcga agcagaatag cccgatgagg aatcttattt
     1441 tttggacaag cttcacagca tattcattcg aggacttcct cagaagccca actgactgag
     1501 cagcgcaagg tgttcccacg agcgcgatat tcttcaccat ctgatcctta accaactcct
     1561 taatggattc caatacctca ttgctcgtat atttgctccc tgcagaccta ttcaattcag
     1621 aagcatcgta aacaacgaaa ggatacgatt tctgtgccca tcggtccaaa cccataacaa
     1681 gcgcacaatc caccagttcc tcttccagcg ccgcagtcaa tagtgcaatg acggctcctc
     1741 cattctggaa gcgttcgtta gctttcacag atctcacatt ctttatgtcg aggtagggtc
     1801 cgatgacatc tgaagaaaca atgctctcga catttgctgg caagcgtgga caggcatcca
     1861 ggcatgctct gcaatcaata catgggttaa cctcacacgg cttattcagc tcatgatcat
     1921 aagccagagt atccgcacgg catacagcga tacatcccct gcagcctgca caaatcccct
     1981 tttgccaaac cttttcctta agctcctgaa aaggttgagg ggtgagctta atcaaatcat
     2041 tttccagcat aaaattgcac ctttggccga tttaaatgct cgtccgagag cgccttccct
     2101 ggatggcccg ccatggagac cccattcctt ttagaacatg aagtcatcac tgccctgatc
     2161 gccaatatca tcctcatcga tatcagatca ctttccacca catgttagca tatgtgatta
     2221 cttttaccat aaaagttagc tattatgttg aacataataa tccttgcgct tgatggcaaa
     2281 tgacgtagga ttgccaccaa gctgccggtt gcgctaatga agcatatgca gctcttgccc
     2341 ataaagcttt gaaaagggat tccaggttaa tccaactcag gattatctga tatgtgtaac
     2401 ttgcgagatc tgcaccgctt ccagcggctt tctgtgggca agagaggcgg ttgtatttct
     2461 ggtagaggag agcggggtgc acagcccgga ggatgcccag cgaatggtgc aaacggggag
     2521 ggacgaatgt cccgctggtg gggtggattc acttcttgag ggaacggagc tgatggcggg
     2581 gctggagggg ccgaagaggt tgtttgaacg gactcgctgg cgcgcctggc tgcacatttt
     2641 tacggggcgc cgagaggcct tccgggacgc aggcaaagag ggcccctcaa cgcc
//
LOCUS       DAOSGP010000188         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_36464_length_2687_cov_14.813070, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000188 DAOSGP010000000
VERSION     DAOSGP010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36464_length_2687_cov_14.813070"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2687)
                     /gene="uvrA"
                     /locus_tag="PLV49_05745"
     CDS             complement(<1..>2687)
                     /gene="uvrA"
                     /locus_tag="PLV49_05745"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG98382.1"
                     /translation="LAFDTIYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAI
                     SIEQKTTSKNPRSTVGTVTEIYDYLRLLFARIGIPHCPEHGTRIESQSPERIAVSILE
                     EMKEKEKGKEKEKGKKMGEGDEESMITILAPVIRQKKGTYGQLLKDLNAEGYARVRLN
                     GQIQRTDEEIELDRYKKHDIDVVIDRLDPREDKSRLVEAIEKALKKTEGLVYVSNEEG
                     KERVYSARMACPVCGLVFEELQPRMFSFNSPFGACESCNGLGFRMEFDPELIIPDRNR
                     CIADGAVALYRNIIDGWRGQHLAAVAKHFGFDIFTPIKDLTPEQYDPLMYGSKEKIRM
                     NMSMKNGDVYWSHNSQWEGLIPQSIRLYSQTASEYRREELEKFMRVLPCPACEGKRLK
                     EKVLAVRIQDLSIVDVANLSVSECNEFFMNLEEKLSVKEGEIARQVLKEIRSRLGFLE
                     HVGLGYLTLSRNAGSLSGGEAQRIRLATQIGSNLTGVLYVLDEPSIGLHQRDNARLIE
                     TLKKLRDLGNTLIVVEHDEDTIRSADHVLDIGPGAGIHGGYVVAEGTPEEIEANPKSL
                     TGQYLSGKKRIEVPAKRRKSNKFIHLMGCRENNLKDIDVDIPLGVLTVVTGVSGSGKS
                     TLIYDTLYRALMKRIYHSNETPGEHDGIRFDTEVDKVIVIDQSPIGRTPRSNPATYTK
                     VFDPIRQAYAQTKEARVRGYKSGRFSFNVQGGRCESCHGDGLIKIEMNFLPDVYIECE
                     ECKGSRYNQETLEVKYKGKSIAEALDMTVEEAREHFENIPAIRSKLDTLLGVGLGYIK
                     LGQSSTTLSGGEAQRIKLTRELAKKGTGRTIYLLDEPTTGLHFDDVKKLIHVLDDLVS
                     KGNTVVVIEHNLDVIKSADYLIDMGPEGGNAGGQVVATGT"
BASE COUNT          475 a          809 c          728 g          675 t
ORIGIN      
        1 gtgccggtgg ccacaacctg gccgccagca tttcctcctt ccggccccat atctatgagg
       61 tagtctgcgg atttgatcac atccaggttg tgctctatca ccactaccgt gttccccttg
      121 gataccaggt cgtccaggac atggatcaat ttcttcacat catcgaagtg cagtccggtg
      181 gtaggctcat ctaaaaggta gatcgtcctg cccgttccct tcttggccag ctctcgcgtg
      241 agctttatcc tctgagcctc gccaccggat agggtggtcg agctctggcc cagcttgatg
      301 tagcccagcc ccacacccag aagggtgtcc agcttgcttc ttatggccgg gatgttctcg
      361 aagtgctccc ttgcttcctc caccgtcata tccagggcct cggcaatgga ctttcccttg
      421 tacttgacct ccaaggtctc ctggttgtag cggctccctt tacattcttc gcactcgata
      481 tagacatcag gcaaaaagtt catctcgatc ttgatcaggc cgtcaccgtg gcagctctcg
      541 caccggccgc cctgtacatt gaatgagaag cgaccgctct tgtagccccg aaccctcgcc
      601 tctttggtct gggcatatgc ctggcgaatg ggatcgaaga ccttggtgta ggtggcggga
      661 ttggatcggg gagtcctgcc gatggggctc tggtcgatga cgatcacctt gtccacctca
      721 gtgtcgaagc gaatcccatc atgctctccc ggagtctcgt ttgagtggta gatcctcttc
      781 atcagcgccc ggtatagtgt atcgtagatc agggtggact tgcccgatcc cgaaacgccg
      841 gtgaccacag tcaggacccc caggggaatg tcgacatcga tatccttgag gttgttctcc
      901 cgacagccca tgagatggat gaacttgttg ctcttcctcc tctttgccgg aacctcgatc
      961 ctctttttgc cggagaggta ctggccggtc agcgattttg ggttggcctc gatctcttca
     1021 ggagtgccct cggccaccac atagccgccg tgaattcctg ctcctgggcc gatgtccagc
     1081 acatgatcgg cactgcggat ggtatcctcg tcatgctcaa ccacaatcag ggtgtttccc
     1141 aggtctctga gctttttcag ggtctctatc agccgggcat tgtccctctg gtgcaggcct
     1201 atggagggct cgtccaggac gtagaggacg ccggtcaggt tcgagcctat ctgggtggcc
     1261 agacggatgc gctgcgcctc cccaccggag aggctgcctg cgtttctgga gagggtcagg
     1321 tatcccaggc ccacgtgctc taaaaatccc aggcgggagc ggatctcctt cagaacctgg
     1381 cgggcgatct ctccctcctt gaccgagagc ttctcctcta aattcatgaa gaactcattg
     1441 cattcgctca ccgagaggtt ggccacatca acaatagata agtcctggat ccggaccgct
     1501 aagacctttt ctttcagcct ttttccctcg caggccgggc agggaaggac ccgcataaac
     1561 ttctccagct cctcccggcg atactcggag gcggtctggc tgtagaggcg aatgctctgc
     1621 ggtatcagcc cttcccattg ggagttatgc gaccagtaaa cgtccccatt tttcatgctc
     1681 atattcatcc ggatcttctc cttcgagccg tacatcaggg gatcgtactg ctcaggggtg
     1741 agatccttga tgggggtgaa gatgtcaaag ccgaagtgct tggccactgc tgccaggtgc
     1801 tggccccgcc agccatcgat gatattacgg tagagggcta ccgctccatc ggcgatgcat
     1861 cgattcctgt ctggtatgat cagctcggga tcgaactcca ttctgaaacc caggccgttg
     1921 caggactcgc aggctccaaa tgggctgttg aaggagaaca tgcgcggctg cagttcctcg
     1981 aaaaccaggc cgcagacggg acaggccatc ctggcggagt agacccgctc cttcccctct
     2041 tcgtttgaga catagaccag gccttccgtc tttttcaggg ctttttctat cgcctccacc
     2101 agcctcgact tgtcttctct cgggtccagc cggtcgatga ccacatcgat atcatgcttc
     2161 ttgtagcggt cgagttctat ctcctcgtcc gtcctctgga tctggccgtt cagcctaacc
     2221 cgggcatagc cctcggcgtt caggtccttg agaagctggc cataggttcc tttcttctgg
     2281 cggatgacgg gggcgaggat ggtgatcatg ctctcctcat ctccctcccc catcttcttt
     2341 cctttctcct tttccttccc cttctctttc tccttcatct cctccagaat gctgacggcg
     2401 atcctctccg gcgactgaga ctcgatgcgg gttccgtgct ccgggcagtg ggggatgcct
     2461 attctggcaa atagcaggcg gaggtagtcg tagatctcgg tcaccgttcc cacagtgctg
     2521 cgtgggtttt tggaggtagt cttctgctca atggagatgg cgggggagag gccgtctatc
     2581 gagtccacgt ccggcttgtc catctggcct aagaactggc gggcgtaggc ggagagcgat
     2641 tccacatagc ggcgctggcc ctctgcgtag atggtatcga aggccag
//
LOCUS       DAOSGP010000189         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_36521_length_2685_cov_19.589354, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000189 DAOSGP010000000
VERSION     DAOSGP010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36521_length_2685_cov_19.589354"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="ilvB"
                     /locus_tag="PLV49_05750"
     CDS             complement(<1..1136)
                     /gene="ilvB"
                     /locus_tag="PLV49_05750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOG98383.1"
                     /translation="MSKGIARTGTDKNRDTPHKMTGAQAVLESLKREGVDVVFGLPGG
                     VLLPLYDAIYKSDIRHILVRHEQGAAHMADGYARATGKVGVCIATSGPGATNLVTGIA
                     NAYMDSVPMVAITGQVNTWLIGKDAFQEADITGITLPITKHNYLIRSAKEIPGVFREA
                     FYIARTGRPGPVLIDLPRDVTVDELVFTWPDVDLPGYHPSFKAHEMQIKKAAKALMEA
                     ERPVIYAGGGVRYASAHKELFELARKVNAPVTTTLMGVGCFPEDHPLSLGMLGMHGTK
                     YANYAIQESDLLMAIGARFDDRVTGKISSFAPHARIVHIDVDPAEVGKNVRVDIPVVG
                     DARNALQGLLKEVSPKPKGPWLEKIDGWKKDHPMRYIPDMNLIKP"
     gene            complement(1121..2644)
                     /locus_tag="PLV49_05755"
     CDS             complement(1121..2644)
                     /locus_tag="PLV49_05755"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOG98384.1"
                     /translation="MCGIDLSGRIRFLDTTLRDGEQTPGVSLGMDEKLAIAQGLDSLG
                     VDVIEAGSAMTSEGERLAIKKIADSGFKAEICSYVRALTADIDTALACDVDSVHLVVP
                     VSDLHIQIKLKADRESVVRMAVDVTEYAKDHGLIVELSGEDASRADQDFLAGLYTAGI
                     EAGADRLAFCDTVGVLVPEVTADIFSRLSRLEAPISIHCHNDFGMATANTVAALRAGA
                     SQAHVTVNGIGERAGNASLEEVVMALESLYQIDTGIRCKDIYQLSRTVSQMTGLLVAP
                     NKAIVGENAFTHEAGIHVHGLLADTKTYEPMQPENVGRKRRIVLGKHAGRASVELAMR
                     EFGLSVTSQQLNQIVTRVKELGDKGKRVSDVDLQSIADTVLSIQMEPKVKLEELTVVA
                     GNTVTPTASVKILLAGNERLEAGVGVGPVDAAINAIRRAISGVADVRLDEYHVDAVTG
                     GTNALVEVWVTMAMADRKITARGAGADIIMASVEAVINGINRLMRLEEEEIARCRKA"
BASE COUNT          558 a          850 c          663 g          614 t
ORIGIN      
        1 ggcttgatca ggttcatgtc cggaatatag cgcatgggat gatccttctt ccagccatcg
       61 atcttttcca gccaggggcc tttaggtttg ggcgagacct ctttaagcag cccctgcagg
      121 gcattgcgcg catcccccac caccggtatg tccactcgca cgttctttcc cacctctgcc
      181 ggatcgacat cgatatgaac gatcctggca tggggagcga agctggagat cttgcccgtc
      241 acccgatcat caaacctggc tcctatggcc attagcagat ccgactcctg gatggcatag
      301 ttggcatatt tagtaccgtg cattcccagc attcccaaag agagcggatg gtcctcgggg
      361 aagcagccca cgcccatgag ggtggtggtc accggagcat tgacctttct cgccagctca
      421 aagagctcct tatgagcaga ggcgtacctc acaccgccac cggcatagat gaccggcctt
      481 tcagcctcca ttaaagcctt cgccgccttc ttgatctgca tctcatgagc cttgaaggag
      541 ggatgatatc ccggcaggtc cacatccggc caggtgaaga ccagctcatc cacagtcaca
      601 tccctgggga gatcgatcag aactggacca ggccgaccgg tgcgggcgat gtagaaggcc
      661 tccctgaata ccccaggaat ctcctttgcg ctgcggatca gataattatg cttggtaatg
      721 ggcagagtga tgccggtaat atccgcctcc tggaaggcgt cctttcctat cagccaggtg
      781 ttcacctgac cggtgatggc aaccattggc accgagtcca tgtaggcgtt cgcgatgccg
      841 gtaaccaggt tcgtggctcc cggcccggag gtggcgatgc atactcccac cttgcccgta
      901 gccctggcat agccgtctgc catatgggct gcaccctgct catggcgcac cagaatatgg
      961 cggatgtctg atttgtatat ggcatcgtaa agaggtaaga gtaccccacc aggaagcccg
     1021 aatactacat ccactccttc tcttttcagg ctttccagca ccgcctgggc gccggtcatc
     1081 ttatggggcg tatctcgatt tttatctgtg cccgttcggg ctatgccttt cgacatctag
     1141 ctatctcctc ctcttccagc cgcatcagac ggttgatgcc attaatcacc gcttccactg
     1201 atgccatgat aatatctgcg ccagctcctc gagctgtaat cttccgatct gccattgcca
     1261 ttgtcaccca cacttctact agggcattgg tcccgccagt tacagcatcc acatgatact
     1321 catcgaggcg aacatcagcc acgcctgata tagcccttct tatggcattg atggccgcat
     1381 ccacagggcc tacgcccact cccgcttcca gcctctcatt tccggcgaga aggatcttaa
     1441 ccgacgctgt gggggttacg gtattaccgg caaccaccgt cagctcttcc agcttcacct
     1501 tcggctccat ctggatggag agcacggtat ccgctatgct ctgcaaatcc acgtccgaga
     1561 cgcgtttgcc cttgtcgccc agctccttta cccttgtcac tatctggttc aattgctggg
     1621 atgtgacaga caggccaaac tcgcgcatag ccagctcaac tgaggcccga ccagcatgct
     1681 tgcccaacac tatccgcctc tttctaccca cattctccgg ctgcatgggc tcataggtct
     1741 tggtgtcagc cagaagaccg tgcacatgga ttcctgcctc atgggtgaag gcgttctcgc
     1801 ccactatcgc tttattagga gccaccagaa ggccggtcat ctgcgagacc gtccttgaga
     1861 gctggtagat gtccttgcat ctgatcccag tatcgatctg ataaagggac tccagggcca
     1921 tcaccacctc ctccaggctc gcgtttcccg cccgttctcc aattccattt acagtcacat
     1981 gggcctgaga ggctccggcg cgaagggcag caactgtatt ggcggtggcc atgccaaagt
     2041 cgttatggca gtgtatactg atgggcgcct ccagccggga gaggcgggag aatatgtccg
     2101 ctgtcacctc cggaaccaac acgcccacgg tatcgcaaaa ggccagcctg tccgcgcccg
     2161 cttcgattcc ggctgtgtaa agacctgcca gaaaatcctg gtccgcgcga ctggcatcct
     2221 cgccgctcag ctccacgatc aagccatggt ccttggcata ctccgtcaca tccacggcca
     2281 tgcgcaccac cgactcccgg tcggccttca gcttgatctg gatgtgaagg tcagacaccg
     2341 gaacgaccag atggactgag tccacatcac aggcgagagc ggtatcgatg tccgcggtta
     2401 aagccctaac atagctgcat atctcggctt tgaagcccga atctgcaatc ttctttattg
     2461 ccaggcgttc tccttcagag gtcattgccg acccggcttc aatgacatcc acccccagag
     2521 aatcaagccc ttgagcgatg gcaagcttct cgtccatgcc caaggatacg cctggagtct
     2581 gttcaccgtc acgtaaggta gtatctagaa atcgtattct acccgataaa tcgatcccac
     2641 acattcatgc tcaatcaccc aacggcgcac aagccgtctc cggcc
//
LOCUS       DAOSGP010000190         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_36571_length_2683_cov_17.950152, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000190 DAOSGP010000000
VERSION     DAOSGP010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36571_length_2683_cov_17.950152"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1659)
                     /locus_tag="PLV49_05760"
     CDS             complement(1..1659)
                     /locus_tag="PLV49_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG98385.1"
                     /translation="MRLQSDFSRPLLAEGERPSTIGGRTAIAGAIAVLLLILSLSFIL
                     LAGSASAGTETGEFCPTCPDWTNLDGWLEKKAAYEEDMKNVQQNEMNNNELKIKTAEA
                     EIGLNAGQNSSFSTAYMGNSSNNNASNSTASSLSRSSELDGAIASPNSIFSDEVILDI
                     SPSAAAYIEGAVNLNYDLFFSEGGEIVPVPEMARLLGDAGISSNDSLLIAGECLPCGG
                     GPSPAFFTYWALKYLGHEKVRVLEGTIEDWKAAGLNISNISATRPKAEYIPHIRPELL
                     ATYEYVVTGGAQIVDARPANAYELGSIPGAINIPYENVLVNDSMMPKEMLKEYFLDLD
                     KNRSVVVYTNIGVEAALLWFALNESGYDARLYTWRDWLENQPKFPYELAEVSAQPNPV
                     RSGEATTITASLIERAERTNSSQYSSSSANSSSNQEPKLTVKGCATCGFGSPQGFANL
                     DRKDGYVQIGSNRKAGQSSASSTNASDQSSSDGILRCTAVIYAPDGGEETRTSLLETS
                     GGRYMGIWNANVAPGVYKVSIVASLDGNAEAFTDVLQVEVTRKG"
     gene            complement(1643..>2683)
                     /locus_tag="PLV49_05765"
     CDS             complement(1643..>2683)
                     /locus_tag="PLV49_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG98386.1"
                     /translation="DSSGWDPMAKLNEIGNVTATQQSAGANWPKKSREIRWGMNSSQG
                     VEDEIGEENESSVASDSIQLAEAEEAKDVQNAARALPNELQINMTRSLKAREMLLPLD
                     GVDDADILLDVSENSSLHIEGSIYIPYMEFFLQPGILKPADEVASILGEAGISREDSV
                     VIYGECLPCGGGPSVATYVYWMMKSLGHEKAKVLDGTAEDWATAGWATSGSAAVLPRK
                     EYIPLMNLNYTATFDLVKSGQVQIIDARTPPEFAMGTIPGSISMPYESVLADKKITGE
                     DRLDMVFMVLNKNQPVVVFTNTGMKGSVVWFALEMMGYDARLYSYQDWMANQLPDLRE
                     VDGSGRLNETAV"
BASE COUNT          549 a          808 c          677 g          649 t
ORIGIN      
        1 tcatcccttc cttgtcacct ccacctgcag cacatcagta aatgcctctg cattcccatc
       61 cagggatgcg actatgctca ccttatacac cccgggagcg acattggcat tccagatgcc
      121 catgtacctg cctccagatg tctccagcag gctggtcctt gtctcttcac cgccatccgg
      181 tgcatatatc actgccgtac acctcaaaat gccatcggag gaggactggt cggatgcatt
      241 tgtggaggag gcagagctct ggcccgcctt tctattcgaa ccgatctgca catatccatc
      301 cttgcgatcc aggttggcaa agccctgggg agagccaaat ccgcaggtag cgcacccctt
      361 aaccgtcagc ttcggttcct ggttggagga ggaattggct gaggaagagc tgtactgtga
      421 tgagtttgtc ctttctgccc tctcaatcaa ggatgcggtt atggtcgtcg cctctcctga
      481 tctgaccgga ttgggctggg cagagacctc cgccagctca taggggaact tgggctggtt
      541 ttccagccag tccctccagg tgtagagccg ggcatcgtag ccggactcgt tcagagcgaa
      601 ccagagcaat gcggcctcaa ccccgatatt ggtgtaaacc acaaccgatc ggtttttgtc
      661 aagatccagg aagtactctt tcagcatctc tttgggcatc atgctatcat tgaccagcac
      721 attctcatag ggtatgttga tcgccccggg aattgagccc agctcataag catttgccgg
      781 cctggcatca actatctgtg ccccaccagt gactacatat tcataggtgg caagaagctc
      841 tggccttata tgggggatgt actcggcctt aggcctggtg gcagagatgt tgctgatgtt
      901 caatcccgca gccttccagt cctcgatcgt gccctcaagc accctgacct tctcatgtcc
      961 caagtatttt aaggcccagt aggtgaagaa cgccggcgac ggtcctccgc cgcagggcag
     1021 gcactccccg gcgatcagca gggaatcgtt gctgctgatg cccgcatctc ccagcagccg
     1081 cgccatctcc ggtaccggca cgatctcccc gccttcggag aagaagagat cgtaattgag
     1141 attgacagcg ccctcgatgt atgcggccgc actggggctg atgtccagta taacctcatc
     1201 cgaaaagatc gaattcgggg atgctatcgc cccatccagc tcgctgcttc tgctcagtga
     1261 ggatgctgtg ctgttgctcg cgttattgtt gctgctattg cccatatagg cggtggaaaa
     1321 ggatgaattc tgaccggcgt tcagcccgat ctctgcttca gcagtcttta tcttcaactc
     1381 attattgttc atttcattct gctgaacatt tttcatatcc tcttcatagg cagccttctt
     1441 ttccagccag ccgtccagat tggtccaatc agggcaggtg gggcagaact ccccagtctc
     1501 tgtgcctgcg ctggccgatc ctgccagcaa aataaaagag agggacagga tcaacaacag
     1561 aaccgcaatc gctccagcaa tggccgttct cccgccgata gttgacggcc tttctccctc
     1621 agccagaagg gggcgggaaa aatcagactg cagtctcatt tagcctcccg gacccatcca
     1681 cctctcggag atctggcagt tgatttgcca tccagtcctg gtagctgtag agccgggcat
     1741 catagcccat catctccagg gcgaaccaga ccacagagcc cttcatcccc gtattggtga
     1801 agacgaccac cggctggttt ttgttcagca ccatgaagac catatccagc cggtcctcgc
     1861 cggtgatctt cttatcggca agaacgctct catagggcat gctgatcgat ccgggtatgg
     1921 ttcccatggc aaactcaggc ggcgtgcggg cgtcgatgat ctgcacctgg ccgctcttga
     1981 ccagatcgaa agtggctgtg tagtttaagt tcatcagagg gatatactcc ttccgaggaa
     2041 gcactgcagc actccctgag gtagcccatc ccgctgtcgc ccagtcctca gccgtcccgt
     2101 caaggacttt tgctttctca tggcccaggc tcttcatcat ccagtagacg taggtggcca
     2161 ccgatggtcc tccgccgcag ggcaggcatt ccccatagat gacaaccgaa tcctctctgg
     2221 agattccagc ctcgcccagg atgctggcga cctcatctgc cggcttcaga atccccggct
     2281 gaaggaagaa ctccatatag ggaatgtaaa ttgagccttc gatatggagg ctcgagttct
     2341 cgctcacatc cagcagaata tctgcatcat cgaccccatc cagcggcaag agcatctccc
     2401 ttgccttgag gctgcgggtc atgttgatct gcaattcatt cggcaatgct cgggcggcat
     2461 tctgaacatc tttggcctct tctgcctctg ccagctggat gctatcggac gctactgaac
     2521 tctcgttttc ctcgcctatt tcatcctcta cgccctggga ggagttcata ccccagcgga
     2581 tctccctgga cttcttgggc cagtttgctc cagccgactg ctgggtagcg gttacattgc
     2641 ctatctcatt aagcttggcc ataggatccc agccgctgga gtc
//
LOCUS       DAOSGP010000191         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_36572_length_2683_cov_15.381659, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000191 DAOSGP010000000
VERSION     DAOSGP010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36572_length_2683_cov_15.381659"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLV49_05770"
     CDS             complement(<1..220)
                     /locus_tag="PLV49_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98387.1"
                     /translation="MNRNKGGAPKSMMDGKQLNQLCKMPQAGQDDKIDPEVMMDCREG
                     PRGQNSENPQNDKIVPKSMMDGKRNPCDQ"
     gene            1115..2176
                     /locus_tag="PLV49_05775"
     CDS             1115..2176
                     /locus_tag="PLV49_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOG98388.1"
                     /translation="MSPASAQTASDTDYTCWQCPVCGYTVILTPAEAESIDPYTLCPN
                     CYSAYAGNFIQVYCSGYVPEDDQSDDPYQDNEQPYWQEDEMNSQDFGDNKNSEDRSSN
                     GLAPALDTVSNKEKILMVLPPDQYQEEELNVPRDYFQSMGYQVHLASKGVKTATGMSG
                     ERTEVDLDLDEVKLSDYIAVVFVGGEGIYSQELNRDPDYQKIAKSASAQKKIVGAICL
                     GPWILADAGLLKGKKATASETDHIKSKGAIVSDDAVVQDGKIITANGPSASQEFAEAV
                     VAALQASSRTDGNSDGLDKTEANAAASSNAASQAAAWRCTVCGYVYDPAENDGVAFED
                     LPGTWKCPCGAPKSKFVRV"
     gene            complement(2302..>2683)
                     /locus_tag="PLV49_05780"
     CDS             complement(2302..>2683)
                     /locus_tag="PLV49_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOG98389.1"
                     /translation="IRIDGTFDYVKTRSVPAQEKPYPPLEEAIKGQEIFEFHDVEGTV
                     VGFRCPDYVEGVNVPGYHLHFITLDRKAGGHLLDLEIEDASAKVDSISGFEMDLPENK
                     QFYQADLSDSQEEELERIESNPRE"
BASE COUNT          657 a          714 c          616 g          696 t
ORIGIN      
        1 tctgatcgca cggattccgc ttgccatcca tcatcgattt gggaacgatc ttatcgttct
       61 gtggattttc cgagttctgg ccacgaggac cttctctgca atccatcatg acctcaggat
      121 cgatcttatc atcctggccg gcctgtggca tcttgcagag ctgattgagc tgcttgccgt
      181 ccatcatgga cttgggtgcg cctcctttat tccgattcat ccggcaatcc tggcatggat
      241 cttccccgca cgaatcttgc ctgccatcca tcatcgactt gggggcaaag ttaccctcat
      301 aattatctgt catggcgttc gttcgaccct gctgctggag atccggagcc gtttcgctca
      361 tcagggactt ctgacagggg gattccatct gctttgcaca ttcctttttt aaagcaattc
      421 cattgtcctg cattgaaagg gctgggacga tggaaatgca tgcgaccaac aatgccgcaa
      481 atagtctaac atatctcatt cactatacct ccactcaaga tcaacgtcag ccctcaggct
      541 tgagttgtct tgcaggcaat cggtaaattt gaacgattta aaaaagtaga accggacgaa
      601 ctgtgagttc attgaacgct gatcccaata actggctaat gagcgtttta aaatcatatt
      661 atgggcttat aatggtttta tttcgcttat tagatggcta atgtattgtg atattgatta
      721 atttcttgaa ttttgatcca aaaagataat aaaatcactc tagtacttta attttgaaat
      781 agagatcaag tggtatttat taagctcttt tatatcgcct taggctcccg atccagctca
      841 acgttcaaat ttcgcccaat acggccacct ctgtctccat agaaagatgc ctttattcct
      901 catctccgcc ccgcgccgca ggaggggatt ttagcattcg atttatagat cgattctaag
      961 agtcactttg cctgaaatgc ggatttgcat cgtgatatcc cttatcatca accacagtaa
     1021 ttcagtttcc ctcatctatt tatcctaatc catcacttca ttcaacatga aaagcccatt
     1081 cctcacacca ctcattctca tcttgctggc tgcgatgtct ccagcatctg cacagactgc
     1141 ttcagataca gactacacct gctggcagtg tcccgtctgc ggatataccg tcatcctgac
     1201 gcccgccgag gccgagagca ttgatcccta caccctctgc cctaactgct attccgcata
     1261 tgccggcaac ttcattcaag tctactgctc aggctatgtt cccgaggacg atcaatccga
     1321 cgacccgtat caggataatg agcagccgta ttggcaagaa gatgaaatga actcgcagga
     1381 tttcggagat aacaaaaact cagaggatag atcgagcaat ggccttgcac ctgccctgga
     1441 tacagtctca aataaagaaa agatcctgat ggttctcccg ccagatcagt atcaagaaga
     1501 ggagctgaat gtgcctcgcg actacttcca gagtatgggc taccaggtcc atctggccag
     1561 caaaggggtg aagacagcca ccggaatgag cggcgagagg acggaggtgg atctggacct
     1621 tgatgaggta aagctctccg attacatcgc cgtggtcttc gtaggcggcg aagggatcta
     1681 ctcccaggag ctgaaccgtg acccggacta tcagaagatc gccaaatcgg ccagcgcaca
     1741 aaagaaaatc gtgggagcca tctgtctggg cccctggatc ctggcggacg ccggccttct
     1801 caagggcaag aaggccactg catctgagac cgaccatatc aagtcaaagg gagcgatagt
     1861 atcagacgat gccgtggtcc aggacggaaa gatcatcact gctaacggcc cctccgcctc
     1921 tcaggagttt gccgaggcgg tagtcgctgc cctgcaggct tcctcgcgca ctgatggcaa
     1981 ttcagacgga ttggataaaa ctgaagcgaa cgcagctgca tcaagcaatg cagcctctca
     2041 ggctgccgcc tggaggtgca cggtatgcgg atacgtatat gatccagcgg aaaatgatgg
     2101 agtggcattc gaagatctgc caggcacctg gaaatgcccc tgcggggccc ccaagagcaa
     2161 gttcgtcaga gtctgaagcc aggaatctgg cacaaaactt ttcttccttt tttgaagatt
     2221 gccaggaatg tgaggatctt ccaatgctac cagctcacac atcacgtaat cattgataat
     2281 aaaaatattt tgcaagatcc tctactccct gggattgctc tctatcctct ccagctcttc
     2341 ctcctggcta tctgatagat ctgcctgata aaactgcttg ttctctggta gatccatctc
     2401 gaagccggat atgctgtcca ctttcgcgct cgcatcctcg atctccaggt ccaggagatg
     2461 gcctcccgct ttgcgatcta aagtgatgaa gtgcaggtgg tagccgggaa cattcacccc
     2521 ttcgacatag tctgggcatc tgaatccgac cactgttccc tcaacatcgt gaaactcgaa
     2581 tatctcctgc ccttttatag cttcttcgag aggcggatag ggcttctcct gggcgggcac
     2641 gcttcttgtc ttgacatagt cgaatgtgcc gtcgatcctg atg
//
LOCUS       DAOSGP010000192         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_36944_length_2664_cov_17.220008, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000192 DAOSGP010000000
VERSION     DAOSGP010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36944_length_2664_cov_17.220008"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..466)
                     /locus_tag="PLV49_05785"
     CDS             complement(65..466)
                     /locus_tag="PLV49_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98390.1"
                     /translation="MNLEEWNLENIREIPGWEGPVSLSEGACRYSKYIRWIRLFINVQ
                     IDEEVDGGRIAFSGGAVGDCPSFEVRRENGQWMRYEIEMAWTPKGEPVLRLRNYSCWD
                     LVYDRISDGTQIDEKIETIEDLAEYLERCLS"
     gene            565..1416
                     /locus_tag="PLV49_05790"
     CDS             565..1416
                     /locus_tag="PLV49_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOG98391.1"
                     /translation="MAEVVQLVEKTAGKEEDGAEDEESIPSAPPRTIWSGSLRIGLVN
                     IPVKAVSMIRNTRISFRMLHKACKTPISFKRYCQEGEEVSLADIVYGYPLGNEKYVVL
                     EKKEIDKAKPESRSTIALDRFVSFFEVDPHYFEKTYLLLPDRSEEAYSLLRAVMEKTG
                     KAAIGRITMHSRERPVIVHYYRQAIAATTMRYPDEIRDPSGYAILKELPEPSEKEMEL
                     GFEIVRGLTGELDYSAYEDEYKKRIEALVRSKMEGVVSVPERKKKEKPPAKSLMEALR
                     ATADSLK"
     gene            1413..2360
                     /gene="ligD"
                     /locus_tag="PLV49_05795"
     CDS             1413..2360
                     /gene="ligD"
                     /locus_tag="PLV49_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOG98392.1"
                     /translation="MNPIKPMLAVSGQPFSREGWIFEPKIDGTRCIASAKKRVLLSNR
                     RLADITYRYPELASALASLQGECVLDGEITVFRKGIPSFRSLAERDHQNDPIKIDYLS
                     RAMPASYVVFDILSLDKESLMHLPLKERKRILFRELEADLKDSETVTLIDSFPEKGED
                     YFQAALKMGIEGVVGKRLESLYLPGIRSQDWVKIKKSLKLDLVVGGYIPGKGDRLPYF
                     GGLLLGAYSQGELVYISRVGSGFTEEELQDITSRFSPREKPPFSDPPATAGVVWIEPE
                     MVVQVTALERTHDGGLRAPVFLRMRDDKEPEECTLDQLG"
BASE COUNT          649 a          693 c          760 g          562 t
ORIGIN      
        1 gcatacttgg cggccatgga gcctgccggg ccggcgccga tgacggctac atcaaagcgt
       61 tctttcagga tagacatctc tccaggtatt ctgccagatc ctctatcgtc tcgatctttt
      121 catcgatctg agtgccatcg gagatgcggt cgtacaccag atcccagcag gaatagttcc
      181 tcagcctgag aacgggctct ccttttggcg tccaggccat ttcaatctca tagcgcatcc
      241 actggccgtt ctctcgtctg acctcaaagc ttgggcagtc tccgacggcg ccgccggaaa
      301 aggctattct tccgccatcc acttcctcat cgatctgtac attgataaac agccttatcc
      361 agcgtatata tttcgaataa cggcaggccc cctccgagag actgacagga ccctcccagc
      421 cggggatctc tctaatgttc tccagattcc attcctccaa attcacatta tccagattgt
      481 gccgagagca tatgcctctt gcgggcggtt aaaaattgcc caaggtgcaa aagattttgt
      541 acaatccagg gataataata ctctatggca gaggtagtgc agctggtcga aaagacagca
      601 ggaaaggagg aagatggggc ggaggacgag gagtcaatcc cctcggcgcc tcccagaacc
      661 atatggtcag gcagcctgcg aataggtctg gtcaacattc cggtcaaagc cgtctccatg
      721 atcaggaaca cccgcatcag ctttcgcatg cttcacaaag cctgcaagac ccccatatcc
      781 ttcaaacgct actgccagga gggggaggag gtctccctcg cggacatcgt ctatggttat
      841 cctctgggaa atgagaagta tgtggtttta gagaaaaagg agatagataa agcgaagccc
      901 gagtcaagga gcaccatagc cctcgaccgc ttcgtcagct tctttgaggt tgatcctcat
      961 tactttgaga agacctatct gctcctgccc gaccgctcag aggaggccta ttctctcttg
     1021 agggcggtga tggagaagac gggcaaagcg gccatcggca ggatcaccat gcactcccgg
     1081 gagaggccgg tcatagttca ttactaccgg caggcgatcg ccgccaccac catgcggtac
     1141 cctgatgaga tacgggaccc gtcgggctat gccatcctga aagagcttcc cgagccatcg
     1201 gaaaaggaga tggagctggg atttgagatc gttcgaggcc tgacaggaga gctggattat
     1261 agcgcttatg aggatgagta caagaagaga atcgaggccc tggtgaggtc aaagatggag
     1321 ggcgtggtga gcgtgccgga gaggaagaag aaggagaagc cccctgccaa gagcctgatg
     1381 gaggctctgc gggcgacggc cgattcgctg aaatgaatcc catcaagccg atgctggctg
     1441 tatccggcca gcccttcagc agagagggct ggatcttcga gccgaagatc gacgggaccc
     1501 gctgcatagc ctcggccaaa aaaagggtcc tgctctccaa ccgcaggctg gcggatatca
     1561 cctatcgcta ccctgagctg gcgagcgctc ttgccagcct gcagggcgag tgcgtcctgg
     1621 acggcgagat cactgtcttc aggaagggca ttcccagttt tcgttctctg gcggagcggg
     1681 accatcagaa cgatccgatc aagatagact acctctcccg cgccatgccg gccagctatg
     1741 tggtattcga tatcctcagt cttgataaag agagcctgat gcaccttccc ctcaaagaga
     1801 gaaagaggat attgttcagg gaattggagg cggacctgaa ggatagcgag accgtgaccc
     1861 tcatcgactc atttcccgag aaaggggagg attattttca ggcagctttg aagatgggga
     1921 ttgagggagt ggtcggcaag aggctggagt cgctctatct tcccggcatc aggagccagg
     1981 actgggtgaa gatcaaaaag agcctcaagc tggatttggt tgtgggcggc tatattccag
     2041 gcaaaggaga tcgattgcct tattttggcg gactgctcct cggcgcctac agccagggcg
     2101 agctggtgta catcagcaga gtgggatcgg gcttcacaga agaggagctg caggatatca
     2161 ccagccgctt ttcgcccagg gaaaagcctc cgttctccga tccgccggca acggcgggag
     2221 tggtatggat cgagccggag atggtggtac aggtcaccgc actggagagg acacatgacg
     2281 gcggcctgag agctccggta ttcttgcgga tgagggatga caaggagccg gaggagtgca
     2341 cactggatca gctcggatag cgaaagaatg caggggaggg gtttggtttt atatccttgt
     2401 tttcggtggc tgctcgttgt ttagcagggg tgaacttgaa tcgccaaaaa atctatatct
     2461 tttcgcctcg gtgatgaacc gctatgctct gacccaacca cagagaaaca gagactataa
     2521 ccgctgccgt acctcatcca aatgattcag cgaagagcca taaaccacaa agcacacaaa
     2581 ggacacaaag accgcaccaa aggattattg ggctatcaag aactggatta acctttgcga
     2641 ggcctttgtg atctttgtgc tcta
//
LOCUS       DAOSGP010000193         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_36988_length_2662_cov_18.964327, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000193 DAOSGP010000000
VERSION     DAOSGP010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36988_length_2662_cov_18.964327"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PLV49_05800"
     CDS             complement(<1..1292)
                     /locus_tag="PLV49_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98393.1"
                     /translation="MKILLLSSPVVQLDFDRIARLPNLGLASLAAHVDDLCTVHVADI
                     HGLKGYREYVKKIVQGYDLVGLTAMSFQYQEALALARIAKEAGAQTVFGGYHPTLAAE
                     EISESDDSQLIDYIVRGEGEATFRELTESLLAGREFKGISGLSYHDPGSYRMIHNPAR
                     PLLDVSDIKMPNRDARLVTRGFYSFDVPIDSVETSRGCTQGCKFCSINLMYGRHFRTF
                     PIERVVEDIRDAEEHGAGSIFFPDDNITLNPKRLERLCQAIIDAGLTHLRYKTQASAS
                     GIASSKRLVDKMGEAGFDGVFLGVESINKRNLEFLGKGKMSNDAEIAVQYLHDNNIIV
                     STGLIGGNPDDDEEDLWENFHLARRLKVDFPIFYISTPYPKTQMRAELEEMGLVTRND
                     FNKYDGMHANLRTKHLSDDEVQYITWEMNARYYDLDWI"
     gene            complement(1320..>2662)
                     /locus_tag="PLV49_05805"
     CDS             complement(1320..>2662)
                     /locus_tag="PLV49_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98394.1"
                     /translation="TAYRNSSEPYDYIGANTRSWFRFYWPRIQSFGLRFLKQNIPEIE
                     ILEFPTWDEYEKKLQEGWDVVGLSFYLSETHEAVEMAEAARSAGAGEVWAGNYGALTT
                     AVQDRFDKVFVGYAEHQLAPYFGRQIKDVIHPPLIEYLNTPFNIKVNIYGVLFTTRGC
                     PVGCKFCQTPVFCNKPNIISLESIERVLAYYKKMGINVVIIEDENFGCNRRHADEVVK
                     LLDKYEMVWGCMARADYLRKKIDDWADARKKNGQQISGFGGAAIGIENLHQERLDEIK
                     KKEGTEDILETLRMLQARGLGTVGYYMIGFEEDTKESLKVDIKKLAALKLDITQICVI
                     TPLPQTPLWKEIEEKYGIFDQDYHHYDGKHLVWNHPQIKPDEMLEILDRSLHQVHSWY
                     VPLRISGRVWYNAYRYGGWEGLKEVAGYISRANKFDWNQGPRFLPLTGNKGSSA"
BASE COUNT          568 a          771 c          660 g          663 t
ORIGIN      
        1 ctgatccagt ccaggtcata gtagcgggca ttcatctccc aggtgatgta ctgcacctcg
       61 tcgtctgaga gatgctttgt cctcaggttg gcatgcatcc catcgtactt gttaaagtcg
      121 ttcctggtca ccagccccat ctcctccagc tccgccctca tctgggtctt cggatagggt
      181 gtggagatat aaaatatagg aaagtctacc ttaagcctgc gggccagatg gaagttctcc
      241 cacaggtcct cctcatcgtc atccgggttc ccgccgatga ggccagtgga gacgatgatg
      301 ttgttgtcat gcaggtactg caccgcgatc tcggcatcat ttgacatctt gcccttgccc
      361 aagaactcca ggttcctctt attgatgctc tccaccccca aaaagacgcc atcaaacccc
      421 gcctcgccca tcttatcgac aagcctcttg ctcgatgcaa tgccggaagc ggaagcctgg
      481 gtcttgtaac ggaggtgagt tagcccggca tcgattatcg cctggcagag cctctccagc
      541 ctcttggggt tcagagtgat attgtcgtcc gggaaaaaaa tcgagccggc accatgctcc
      601 tctgcatccc ggatatcctc gactaccctc tcaatgggga aggtgcgaaa atggcgtccg
      661 tacatgaggt tgatggagca gaacttgcag ccctgagtgc atccgcggct ggtctccacc
      721 gagtcgatgg gcacatcaaa gctgtagaag ccccgggtga caaggcgggc atcccggttt
      781 ggcatcttga tatccgatac atccaagaga ggcctggccg gattgtgaat cattctatag
      841 ctgcccggat catgatagga gaggcccgag atccccttga actctcttcc tgccagcagg
      901 gactctgtca gctcccggaa ggtagcctcg ccttcacccc gcacgatata gtcgatgagc
      961 tggctgtcat cgctctcact gatctcctct gccgcgagag tgggatgata gccgccaaag
     1021 accgtctgag cccctgcctc tttggcgatc ctggccagag ctagtgcctc ttgatactgg
     1081 aagctcatag cagtcaggcc gacgagatca tatccctgga caattttttt cacatattcc
     1141 cggtaacctt tcaggccgtg aatatctgcc acatgaactg tacacaggtc atccacatgc
     1201 gccgccaggc tggccagacc cagattgggc aggcgggcta tgcggtcgaa gtccagctga
     1261 accacagggg aggataggag gagtatcttc attcagaacc ttcgtttatt cgttcgtatt
     1321 catgcggatg atcctttatt tcccgtcaat ggaaggaacc tgggcccctg attccagtcg
     1381 aacttgtttg cccgggagat gtaaccagca acctctttta gcccttccca tccaccatag
     1441 cgataagcat tgtaccagac tcttccagag atgcgcaggg gcacatacca ggaatggacc
     1501 tggtggagcg accggtctaa gatctccagc atctcatccg gtttgatttg gggatggttc
     1561 cagaccagat gctttccatc atagtgatga tagtcctggt cgaatatgcc atacttttcc
     1621 tcgatctctt tccacaatgg ggtctggggc aaaggagtaa tgacgcatat ctgggttatg
     1681 tccagcttga gagcagccag cttctttatg tccaccttta gcgactcctt tgtgtcctcc
     1741 tcaaagccga tcatatagta gccaaccgtc cccaggcctc gagcctgcag cattctcagc
     1801 gtctccagaa tatcctcggt gccttccttc ttcttgattt cgtccagacg ctcttgatgc
     1861 aggttctcta tgccgatagc cgccccgcca aagccggata tctgttggcc gttcttcttc
     1921 cgggcatcag cccaatcatc gatcttcttt ctcagataat cagcccgggc catgcacccc
     1981 cagaccatct catacttgtc caggagcttc acaacctcat ccgcatggcg gcggttgcag
     2041 ccgaagttct catcctcgat tatcaccaca tttatgccca tttttttgta gtaggcaagg
     2101 accctctcga tgctctccag ggagattata ttgggtttat tgcagaaaac cggagtctgg
     2161 cagaacttgc agcccactgg gcagcctcgg gtagtgaaaa ggaccccata gatgttcacc
     2221 ttgatattga agggcgtatt cagatactcg ataagcggcg gatggatgac atcctttatc
     2281 tgcctgccga aatagggcgc cagctgatgc tccgcatatc ccacaaagac cttgtcgaac
     2341 ctgtcctgca cggcggtggt tagggctcca tagtttcctg cccatacctc tcctgcgccc
     2401 gcactgcgtg ccgcctctgc catctctact gcttcgtggg tctcgctaag gtaaaaggag
     2461 aggccaacaa catcccagcc ctcttggagc ttcttttcat attcatccca ggtggggaac
     2521 tccagtatct caatctccgg aatgttctgc ttcaggaatc tcaggccgaa ggattgaatg
     2581 cgcggccagt aaaatctgaa ccacgaccgg gtgttggccc cgatgtagtc gtatggctca
     2641 gagctatttc ggtaagcggt tg
//
LOCUS       DAOSGP010000194         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_37088_length_2657_cov_19.193697, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000194 DAOSGP010000000
VERSION     DAOSGP010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37088_length_2657_cov_19.193697"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="PLV49_05810"
     CDS             complement(<1..1341)
                     /locus_tag="PLV49_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HOG98395.1"
                     /translation="MSIFGVDIASGSPGSRRAPSYSLVILEGDATALHHMISRHKLIR
                     MIRERQPEVVAMDNVHELAQSHKDLINLLRRLPPSTKLVQVTGGERPESLVRLARWNG
                     ITFDRTKPLEEAEASARLAARGVGAVVSAFEDKTLIKVSRRRSLGRGGWSQNRYTRKI
                     HGAVMAMAREVEKQLRESGLEYSLRTVEGLGGFTRAEFVVEASRDKVHINPGYSVDAQ
                     LKVQSIERPALQYKPLLHRRGYIIVGLDPGTTTGIAALNLRGELVDLISSRAMSSSDV
                     IEWIAARGRPLIVATDVSPTPGAVEKVKRAFNAVLFSPGADMAGEEKIALGREVGYKN
                     DHERDALAAALSAFRKYKNKFIQVEKKAPAEVDPDEIKALVVRGYSIENAIAEFTQPT
                     PAEGRPAAPAASGAPAPDPDTAGLRQHIQQLSEQVKTLRTYVDELQAQLAEKDAD"
     gene            complement(1338..2207)
                     /locus_tag="PLV49_05815"
     CDS             complement(1338..2207)
                     /locus_tag="PLV49_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HOG98396.1"
                     /translation="MENVSEAFLGLSREDFSLLGAIETGMRTHEWVPTFIISKLAGLS
                     ASKSEYRLQHLFEKKLVVREAQHYLGYQINFDSYDLLALYDFVRRDQVKSIGERIGVG
                     KESIVLGAQGYASLAIKFHRQGRTSFKHVRRLRDHLKDKPRVPWLYAASLAARREYQV
                     MEKLYSKVSIPRPVAISRHALAMEYVPGPLLNRITLEDPEDGLRLILGEVGRALGQGI
                     VHADLSEFNIIITDSGPVIIDWPQAVDAAHPHADELLKRDLGNVLRFFQRKYRIDMPL
                     EEALSVVREADRV"
     gene            complement(2233..>2657)
                     /locus_tag="PLV49_05820"
     CDS             complement(2233..>2657)
                     /locus_tag="PLV49_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOG98397.1"
                     /translation="FKVIVLPNLYGDILTDEAAEFQGGVGTAGSANIGKRYAMFEAIH
                     GSAPRMVDEGRAQYADPASMMRACVMMLRHISFIDQASRMEMALDICGQFEKRVCLTG
                     RPDGATGAQYADYVLETVQDPDLQNRWQRYQASGEVKL"
BASE COUNT          515 a          866 c          729 g          547 t
ORIGIN      
        1 atcggcatcc ttctccgcca gctgggcctg cagctcatcc acgtaagtgc gcagcgtctt
       61 cacctgctcg gatagctgct ggatatgctg cctcaggccg gcggtatccg gatctggagc
      121 aggggccccg gaggccgccg gggcggctgg ccttccctcc gccggggttg gctgggtgaa
      181 ctcggcgatg gcgttctcaa tggaatagcc ccggaccacc agtgccttga tctcatcggg
      241 atctacctcc gccggagcct tcttctccac ctggatgaac ttgttcttgt acttcctgaa
      301 ggcagacaga gcagcagcca gagcatccct ctcatggtcg ttcttgtagc cgacctctct
      361 gcccagggca atcttctcct ccccggccat atccgccccc ggactgaaca gcacggcatt
      421 aaaggccctc tttaccttct ccaccgcccc cggcgtgggc gagacgtcgg tagccacaat
      481 cagcggcctt cccctggcag ctatccactc gatcacatcg gaagaggaca tagcccggga
      541 gctgatcagg tcgaccaatt ccccccgcag gttcagggcg gcaatgccgg tggtggttcc
      601 tgggtcaagg cccactatga tgtagcccct gcggtgcagc agcggcttgt actgcagagc
      661 aggcctctca atgctctgca ccttcagctg ggcatcgacc gaatagccgg gattgatgtg
      721 caccttatct cgggaggcct ccaccacgaa ctccgcccgg gtgaagccgc ccagcccctc
      781 caccgtccgc agagagtact ccaaaccaga ttcgcgcagt tgcttctcca cctcccgcgc
      841 catggccatc acagctccgt ggatcttgcg agtatagcgg ttctggctcc agccgccgcg
      901 tcccagggac cgcctccggc tcaccttgat caatgtctta tcctcaaatg cggagaccac
      961 ggccccaacg cccctggccg ccagcctggc cgaggcctcc gcctcctcca ggggcttggt
     1021 gcggtcgaag gtgatgccat tccagcgggc cagacggacc agggactccg gccgctcacc
     1081 gcccgtcacc tgaaccagtt tggtcgaagg ggggagacgg cgcaggaggt tgatcagatc
     1141 cttgtggctc tgggccagct catggacgtt atccatggcc acgacctccg gctgccgctc
     1201 cctgatcatc cggatcagct tatgcctgct gatcatatga tgaagggcag tggcatcccc
     1261 ctccaggatc accagtgagt agctgggagc cctgcgactg cctggtgagc cgctggcgat
     1321 atccaccccg aagatgctca aaccctgtcc gcctcccgga caaccgagag cgcctcttcc
     1381 agcggcatat cgatgcggta ctttctctgg aaaaagcgaa gcacattgcc cagatccctc
     1441 ttaaggagtt catctgcatg aggatgagcg gcatccaccg cctggggcca gtcgatgatc
     1501 accggcccgg aatcggtgat tatgatgttg aactcgctca ggtcggcatg aactatcccc
     1561 tgacccaggg cccgtccaac ctcacccagg atcaacctca ggccatcctc gggatcttcc
     1621 agagtgatcc tgtttaaaag cggcccgggg acatactcca ttgccagagc atgccggctg
     1681 atggccaccg gccgggggat ggaaaccttc gagtagagct tttccatcac ctgatactcc
     1741 cgtctggctg ccagagaggc ggcataaagc cacgggaccc gaggcttgtc cttgaggtgg
     1801 tcgcgcaggc ggcggacgtg cttgaagctg gtccgtccct ggcggtggaa cttgatggct
     1861 aaagaggcgt atccctgggc cccaaggaca atagactcct tgcccacccc tatccgctct
     1921 ccgatgcttt ttacctgatc gcgccggacg aagtcataga gcgctaaaag gtcgtaggaa
     1981 tcgaagttta tctgatagcc caggtaatgc tgagcctctc gcaccaccag ctttttctca
     2041 aaaagatgct gcaggcgata ctctgatttg ctggcggaga ggccggcaag cttagatatt
     2101 ataaaagtgg ggacccactc gtgggtcctc atcccggttt caatcgcgcc cagcagagag
     2161 aaatcctctc ggctcaagcc cagaaatgct tctgaaacgt tttccacgag caatattatg
     2221 gaaccacttc agctaaagct ttacctctcc agaagcctga tatctctgcc agcgattctg
     2281 tagatctgga tcttgaacag tctcgagcac atagtcagca tactgggctc cagtggcacc
     2341 atctggcctg ccggtgagac ataccctctt ttcgaactgg ccacagatat ccagcgccat
     2401 ctccatccgg ctggcctgat ctatgaagct gatgtggcgc agcatcatca cgcaggccct
     2461 catcatgctc gccggatcgg catactgcgc tctgccctcg tcgaccatgc gcggagctga
     2521 gccgtggatg gcctcgaaca tggcatacct cttgccgata ttggcgcttc cggcggtgcc
     2581 cactccgccc tggaactccg cggcctcatc ggtgaggatg tccccataca ggttgggcag
     2641 gacgatcacc ttgaagt
//
LOCUS       DAOSGP010000195         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_37240_length_2650_cov_15.131792, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000195 DAOSGP010000000
VERSION     DAOSGP010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37240_length_2650_cov_15.131792"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..687
                     /locus_tag="PLV49_05825"
     CDS             235..687
                     /locus_tag="PLV49_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98398.1"
                     /translation="MPSTKYTRIEITPEAYRALEAEAILQEKTLKKLASELILRGISK
                     EALDFIKKAGESKKSRRALDSSAMERAIEEIGATGMSFDQSILENMHDIIQDEGYSEG
                     MLYAVQNTASMQRDELHRVLNICERHGLTNILAADIILNLNKIESGTR"
     gene            complement(714..1709)
                     /locus_tag="PLV49_05830"
     CDS             complement(714..1709)
                     /locus_tag="PLV49_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOG98399.1"
                     /translation="MIDTDLSIILFLQGQDWATPIMRLASSLGSLEFMLLSMPFLYWC
                     WDAKQGFMIGLMLVTSHGLNAVLKIALHCPRPYWVDPNVQAWSTEPSFGMPSGHAQNA
                     VSIWGLTAQLIHKRWAYLLAAGTCLLIGLSRAYLGVHFMGDVLAGWGVGAVLLGSSIK
                     AMPVMEEKIGKMDLKGQILLATLASLAIIALYLLSLAGTGAWQMPSAWEANALAATGE
                     PIDPFRPVDAFCAAGMMLGISSGYAILKRRGGFLADGPLSRRLVRYLLGMIGVVLIWY
                     GLKGAMQIEAADWALDYIRSMLAGLWVTLGAPLMFIGLGLAKREQVDGQSASGCP"
     gene            complement(1717..>2650)
                     /locus_tag="PLV49_05835"
     CDS             complement(1717..>2650)
                     /locus_tag="PLV49_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOG98400.1"
                     /translation="SIIKYVYIRYIGSALAENIWYIISTIISISIIIILTILIARLVN
                     GLLEKQIPKIMESSRLGKEFDETSHALTRRLIVAAIYTVGILMTILLIPSLNRIAIAM
                     LAGAGVATLAIGFAAQDSLSNIISGIFLAIFKPMRIGDYVDFKGEYGYVEDITLRHTI
                     ICTWDQRRIIVPNKLMGSDFIVNWSIGNPETVWPVNFGIGYSADIDKAKSIITEVAES
                     HPHVLKEKGINVRLTELGDFAMNLRLTFYAPSRGMAYDAGCDIREAVKKRFDAEGIEI
                     PYPYQNVIIMKQPVDVSSCPGPENDHSQEEEQAN"
BASE COUNT          657 a          704 c          691 g          598 t
ORIGIN      
        1 cctctgccgc tgcatccaat ccgcttccct cgatctgcat taaccatcgc ttgtacgaaa
       61 agcttttgga tgaatgatct caaaccagct ttaccataat ccattatgat cgtctggtgg
      121 gattggctgc cggagacggt ccctccccta caatttttcc gcaaatgatc gcagtctatt
      181 aataggaata attatattag cactttcgcg agaaaaccga ggcgcagcgg ataaatgccc
      241 agcacaaaat ataccagaat agagatcacc cccgaggctt acagggccct tgaagccgaa
      301 gctattctgc aggagaagac actgaagaag ctagcttccg agctgatctt gcggggcatt
      361 tccaaagagg ctctcgattt tatcaagaag gcaggggaat caaaaaaaag tcgccgggca
      421 ctggacagca gcgccatgga gagggctatt gaggagatcg gggccaccgg tatgagcttc
      481 gatcagtcca ttctggagaa catgcacgat atcatccagg atgagggcta ttccgaggga
      541 atgctttatg ccgttcagaa caccgcctct atgcagagag atgagcttca ccgggtgctg
      601 aacatttgcg agcgccacgg cctgaccaac attctggccg cggatatcat cctgaacctg
      661 aacaagatcg agtccggcac gagatgagaa tttgatcaaa atcggctcgc ggattatgga
      721 catccgctcg cactctgccc atcaacctgt tcccttttgg ccaggccaag gccgatgaac
      781 atcaatggcg cccccagtgt gacccagagc cctgccagca tgctcctaat gtagtcgagg
      841 gcccagtcgg ctgcctctat ctgcatcgcc cctttcaatc cataccagat caggaccact
      901 cctatcattc ccagaagata gcggactaga cgcctggata gtgggccgtc tgccaagaag
      961 cctcctctcc tctttaaaat ggcatagccg gaagagatac caagcatcat ccccgcagca
     1021 cagaatgcat ccaccgggcg gaatggatcg atgggctctc cagttgcggc cagagcattg
     1081 gcctcccagg cagagggcat ctgccaggca cccgtgccgg ccaggctcag gagataaagg
     1141 gcgatgatag caagagatgc cagggttgcg agcaggatct gtcctttaag atccattttt
     1201 ccgatcttct cctccataac tggcattgcc ttgatggatg agcccagaag cactgcaccc
     1261 acgccccagc ctgcaagcac atctcccatg aagtggaccc ccagatacgc tcgagagaga
     1321 ccgattaata agcaggtgcc agcggccagg agataggccc agcgcttgtg gatgagttgt
     1381 gcggtcaggc cccagatgga gacggcattc tgggcgtgcc cggagggcat gccaaaggag
     1441 ggttcagtgc tccaggcctg gacattggga tcgacccagt agggccgggg gcagtgcagg
     1501 gcgatcttca ggacggcatt cagcccatgg ctcgtgacca gcatcagccc tatcataaag
     1561 ccttgctttg catcccagca ccagtacagg aagggcatgg aaaggagcat gaactccagc
     1621 gatcccaatg aggaggccaa tctcattatg ggggtggccc agtcctggcc ctgcaagaat
     1681 aagataatgg agaggtccgt atctatcata tttagattaa ttggcttgct cctcttcctg
     1741 agagtgatca ttctccggtc ctggacaaga tgatacatcg accggctgct tcatgattat
     1801 gacattctga tatggatacg gaatctcaat tccctctgca tcaaaccttt ttttgaccgc
     1861 ttcccttatg tcgcagccgg catcatatgc catcccccgg ctgggggcgt agaaggtcag
     1921 gcgcaggttc atggcaaagt ctcccagctc ggtgaggcgg acattgatgc ccttctcctt
     1981 cagaacatgg ggatggctct cggctacctc tgtgatgatg ctcttggcct tatcgatatc
     2041 cgctgagtag cctattccaa aattaaccgg ccagacagtc tccggattgc cgatgctcca
     2101 gttcacaatg aagtcagagc ccatcagctt gttcggcact attatcctcc tctggtccca
     2161 ggtgcagatt attgtatggc gtagggttat atcctcgaca tagccgtact cgcctttgaa
     2221 gtcgacgtag tcgccaatgc gcatgggctt gaagatggcc aaaaagatcc cggatattat
     2281 attggagagg gagtcctggg cggcaaaacc gatggccagg gtcgccacac cagcaccagc
     2341 cagcatggcg atagcaattc tgttcaggga ggggatgagg agaatggtca ttaaaatgcc
     2401 gacggtatat atcgcggcaa cgatcagcct gcgggtcagg gcatgggagg tctcatcaaa
     2461 ctctttgcca agccggctgg attccataat tttagggatc tgcttctcca gcagaccatt
     2521 gaccagccgg gcgattaaaa tagttaagat gataattata gatatggata tgatggtcga
     2581 tataatgtac catatgttct ctgccagagc gcttccaata taacgtatat atacgtattt
     2641 tattattgat
//
LOCUS       DAOSGP010000196         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_37404_length_2642_cov_17.545419, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000196 DAOSGP010000000
VERSION     DAOSGP010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37404_length_2642_cov_17.545419"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PLV49_05840"
     CDS             complement(<1..1286)
                     /locus_tag="PLV49_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98401.1"
                     /translation="MILILAVSTAQATVWGQGVSGELHWKEQLTLEDYTLTLADFSLE
                     EKLMVLVELQEDERPIARRALHAGEWFVINDSLRISALKIVEGADKDEPYAIVRMQLP
                     SAPEISLILSFDNDIYRGGEDIKILLSVENRGAVDAEDLKITVDSSPPIFSRRFNISS
                     LLAGSAWDDKKKTAQTDPIRIDLVAPYMLEAGDLQVRVRAQYTDPDGNSHQSWGGGKA
                     HISGPLRLYKRVEETQELSESYYVINSLSNYGNRTFSVDLSDSTGKEFHSNDTLQWKI
                     VLEPGQTKTVSYCIKPIEPAMGISLPDAVASFRMGKRIYTIRSNRPVVDVTGPLIDAK
                     RSISPKSVKAGEEVVISLELENRGNRKAVLTMAEMVPQGAELISGELNESFMLTPGEK
                     AARKIRLRVRDPDGISIPTSMVRYRDVRGNDYSTRTS"
     gene            complement(1303..2433)
                     /locus_tag="PLV49_05845"
     CDS             complement(1303..2433)
                     /locus_tag="PLV49_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98402.1"
                     /translation="MRCSPVPRRKGATLAIILTALFGASILLSIIIPTQICLASGEET
                     NLSTDAAPSNLTDGNTSAKEGIPVHVYITNNDNEKLDVSLFIDSELIDRKSISSGADQ
                     KIDSYPLEKGSHTFKISWWDEDVKSSFEVEEIKDITAETSVNLYTTLNEEPEEFEITV
                     NLVNENSRELEAFLYADGSFEKSKKVGKESSAELGKINLKEGIHNLSVRWQDDDTRIE
                     YEKTKKLMVKRDEVVIFYAPKGVSFEAKNASSGADDRSSGDSDKKTSIKDEQIDKEDP
                     VDEPDAGSENASKTDSTSAEVDDEKKGAKGDVIETKDEASSRYGIGSSASTDNPLQDS
                     SGGKSSPAKNANSMIGGSLEDGNRLYIYAALIMLTIYLLLRH"
BASE COUNT          578 a          724 c          602 g          738 t
ORIGIN      
        1 gaagttctgg tgctataatc attcccccga acatcccgat atctcaccat ggaggtggga
       61 atgctgattc catccgggtc tctcacccgc agccgaatct tccgggcggc cttctcgcct
      121 ggggtgagca tgaaggattc attaagttct cccgagatca gctctgcccc ctggggaacc
      181 atctccgcca tcgtcagcac cgcttttctg ttgcctcgat tctccagctc caaagatatc
      241 accacctctt cccctgcctt tacactcttg gggctgatgc ttctcttggc atcgatcaag
      301 ggtccggtaa catctaccac cggcctgttg gagcggatgg tgtaaatcct cttccccatg
      361 cggaacgagg ctacggcatc aggaagagag atgcccatgg ctggctctat gggcttgata
      421 caatagctga ccgtcttcgt ctgacccggc tcaagaacga tcttccactg gagggtgtca
      481 ttgctgtgga attcctttcc cgtggagtcg gacagatcca cggaaaatgt cctgtttcca
      541 tagttgctga gggaattgat cacatagtag ctttcagata actcctgtgt ctcttcaacc
      601 ctcttgtaaa gccggagagg gccggatata tgcgctttgc cgccgcccca tgactgatgg
      661 gaatttccat ccggatcagt gtattgagcg cggaccctga cctgcagatc gcccgcttcg
      721 agcatatatg gagccaccag atcgatcctg atgggatcgg tttgggccgt ctttttctta
      781 tcatcccagg cggatcccgc caaaaggctt gatatgttga acctcctgct gaatatcggc
      841 ggactggaat cgacggttat cttcaggtct tctgcatcaa cagcgcctcg attctccacg
      901 gacagtagta ttttgatgtc ctccccgccc cggtagatat cgttatcgaa gcttaaaatc
      961 agagatatct ccggggcgct gggaagctgc attctcacaa tggcataagg ctcatccttg
     1021 tccgctccct ctacgatctt cagggcgctg atcctcaagg aatcgtttat gacgaaccac
     1081 tcgccagcat gaagagcccg gcgagcaatg gggcgttcat cctcctgcag ctccaccaag
     1141 accatgagct tctcctccag ggagaagtcg gccagggtca gggtgtagtc ctccaatgtc
     1201 aattgctctt tccagtgaag ctcgcctgag accccctggc cccagactgt tgcctgagcg
     1261 gtggagaccg ccaagatcag gatcaaaaag gctctgatca tctcaatgcc ttaagagcag
     1321 atagatcgtc agcatgatca atgccgcata gatgtacaat cgatttccgt cctccaatga
     1381 gcctccgatc atggagttgg cattctttgc tggggagctt tttccccccg agctatcctg
     1441 cagagggtta tctgtgctgg cactcgatcc aatcccatac cggcttgacg cttcatcctt
     1501 cgtctcaata acatcgccct ttgctccctt cttctcatca tcgacctcag cagaggtact
     1561 atcggtcttt gaagcattct cactgcctgc atccggctca tcgacgggat cctctttgtc
     1621 gatctgctca tcctttatgc tcgttttctt atcgctatca ccagaggagc gatcatctgc
     1681 accagaggag gcattcttag cctcaaagga tacccctttg ggggcataga atatgaccac
     1741 ctcatcgcgc tttaccatta gctttttcgt cttttcatac tctattctgg tatcatcatc
     1801 ctgccatctg accgatagat tgtgaatgcc ctcttttaga tttatcttcc ccagctcagc
     1861 gctgctctcc tttcccacct ttttgctctt ctcgaagctg ccgtctgcat agagaaatgc
     1921 ctccagctcc cgggagttct cattgaccag attcactgtt atctcaaact cctccggctc
     1981 ctcattgagg gtggtgtaaa ggtttaccga tgtctcggcc gtgatatcct ttatctcctc
     2041 cacctcaaag gaactcttca cgtcctcatc ccaccagctt atcttgaagg tatgactccc
     2101 cttctccaga gggtagctgt ctatcttttg atccgctcct gaggagatcg acttgcggtc
     2161 tatcaattcc gaatctataa acagggagac atcaagcttc tcattgtcat tgttcgtgat
     2221 atagacgtgt actgggattc cttcttttgc cgatgtattc ccgtccgtca ggttcgatgg
     2281 tgcagcatca gttgagagat tcgtctcctc cccgctggca aggcatatct gggtgggaat
     2341 gatgattgaa agcagaatgc tagccccgaa aagagcggtg agaataatgg ccaatgttgc
     2401 tccttttctc ctgggaactg gactgcacct catgtatatc aattatgcaa tgatgcttta
     2461 aatatcattt ggtaagcaaa aaattaagat tcatgttaat attagtctat caaatagatt
     2521 ttaaatcaga tattttattc ttcataacga aagtattatc tacgagaggt taagataaag
     2581 cccatcgaga ggatgagata ttgatggata ctgccatctt gcttctggta gtggagggag
     2641 tg
//
LOCUS       DAOSGP010000197         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_37588_length_2634_cov_16.667313, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000197 DAOSGP010000000
VERSION     DAOSGP010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37588_length_2634_cov_16.667313"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PLV49_05850"
     CDS             complement(<1..505)
                     /locus_tag="PLV49_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG98403.1"
                     /translation="MSQAAVRNETNSQPLTLTVQGGVDKCGHQDLVHEIEISRGEIIG
                     IVGPTGSGKSTLISDIEQFALGDTPTGRSILINGQVPKNEQRYDPRKKLVAQLSQNMH
                     FLADMQVGEFLRMHAKSRGKDPNQADKVIELANTLTGEPISPEFQLTILSGGQSRALM
                     VADIAVIS"
     gene            complement(577..1800)
                     /locus_tag="PLV49_05855"
     CDS             complement(577..1800)
                     /locus_tag="PLV49_05855"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HOG98404.1"
                     /translation="MGEYLLRCVSDGSVRPPYSLSCPHDSSLLRAEYKSRQLEIKDLP
                     GIWRFIDWLPVKGIIEKATGRSVTYKSQGLARELDLENLYISFNGYWPEKYASLPTCS
                     FKDLEAAPTMQMLLDKGERSSLVVASAGNTARAFAHIASLTGQELVLFVPEKALERMW
                     TIVPPGRITLIAVKGDYLDAIQMAEKVQSRKGFTPEGGARNIARRDGMGTVMLDAAVT
                     IGRTPDHYFQAVGSGTGGISAWEASLRLKNDGRFHPELPLPRLHLAQNIPNAPIYYAW
                     KALEPEPYQEEMFDDVLFNRKPPLDMPGGVRDALKATEGMVYGITDREASKARDLFEQ
                     SEEIDILNAPAVAVAALQMAMEAKSVSSKDIILLNITGGGMARMKEDHSRHMLQPDVA
                     VSSWEDAALFLEEGS"
     gene            1942..2457
                     /locus_tag="PLV49_05860"
     CDS             1942..2457
                     /locus_tag="PLV49_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG98405.1"
                     /translation="MPNVERVMRAAFQSDESLRDTLKEVIGELGLSAREFSRISEIPQ
                     STLYKILSGHREPNITTLRQIVKTIRQLEGSGGDFIAIIAARPVLDKISEKKMKIGER
                     LLTLREYSATNIEEAIIAAVRAERDGASALVCAPIVSPTVEKLLTIPVATIIPQESVV
                     RAIEVAARKIE"
BASE COUNT          573 a          734 c          690 g          637 t
ORIGIN      
        1 cgctgataac agcaatgtcc gccaccatca gggcccggga ttggccgccg ctgagaatgg
       61 tgagctgaaa ctcggggctt atgggctcgc cggtcagggt attggccagc tcgatgactt
      121 tgtccgcctg attaggatct ttgccccgag acttggcatg catccgcagg aactctccca
      181 cctgcatgtc tgccaggaaa tgcatattct gggagagctg ggcgaccagc ttctttcgcg
      241 ggtcatagcg ctgctcgttt ttcggcacct ggccattgat cagaatgctg cgcccagtag
      301 gtgtgtctcc cagggcaaac tgctctatat cggagataag agtgctcttg cctgagcccg
      361 tgggccccac gattcctatt atctctcccc gggagatctc tatctcatgg acaagatcct
      421 ggtggccgca cttgtccact ccaccctgaa cggtgagcgt caagggctga ctgttggtct
      481 cattgcggac cgctgcctga ctcattgcag cgcccatcgg ctgatcagct atatcagcta
      541 tccttttccg gctcattgta tcgctaatag tctggctcac gatccttcct ccagaaatag
      601 ggctgcatcc tcccaagagg agactgcaac atccggctgc agcatatggc gggaatgatc
      661 ctctttcatc cttgccatgc cgccgccggt tatattgaga agtatgatat cctttgagga
      721 gacgctcttc gcttccatcg ccatttgcag cgcggccaca gcaacggcag gggcattgag
      781 aatatctatc tcctcagact gctcgaatag gtccctggct ttgctggcct ctctgtctgt
      841 gattccgtaa accatgccct ccgtggcctt cagggcgtct ctgactccgc cgggcatgtc
      901 caatggaggc ttgcgattga acaggacatc atcgaacatc tcctcctggt acggctcggg
      961 ctccagagcc ttccaggcat agtagatggg ggcattgggt atattctggg ccagatgcag
     1021 cctgggcaaa ggaagttccg ggtggaaccg gccatcgttt ttcagccgca ggctggcctc
     1081 ccaggcggat atgcctccag tgccgcttcc caccgcctga aaatagtgat cgggcgtgcg
     1141 tccaattgtt accgctgcat ccagcatcac cgttcccatt ccatcccggc gggcgatatt
     1201 cctcgcccct ccctcggggg taaaaccctt tcgggattgc accttctctg ccatctgaat
     1261 ggcatcgagg taatcgcctt tgactgcgat cagagttatt ctgcctggtg gtacaatcgt
     1321 ccacattctt tccagggctt tttccggaac aaataagacc agctcctggc cagtcaaaga
     1381 tgctatatgg gcaaatgcac gggcggtgtt tcctgctgag gccaccacca gtgatgacct
     1441 ttccccctta tccaggagca tctgcatggt tggcgcagct tccaggtcct tgaagctaca
     1501 ggtgggcaaa cttgcatact tctccggcca gtagccattg aagctgatat aaaggttctc
     1561 aagatcaagc tcccttgcca gcccctggct tttgtaggtc actgaccttc cggttgcttt
     1621 ctctataatc cccttgacag gaagccagtc aatgaaccgc cagataccgg gaaggtcctt
     1681 tatctccagc tgcctggatt tgtactccgc ccgcaacagc gagctatcat gagggcagct
     1741 aagggaatat ggaggccgaa ccgatccatc gctcacgcat ctgagcagat attctcccat
     1801 gagagatacc ttcaccaaag ggaatataac attttctatt tgtgaccata ttttatcctt
     1861 tttgtacaag aattatatct ggcaggcacg tttaggttat atcccaaaag tacaaatatt
     1921 agctgttgca tacccaatag tatgccaaat gtggaaaggg tgatgagagc tgccttccaa
     1981 tcggatgaga gcctgcgtga caccttaaaa gaggttatcg gggagctggg gctctctgcg
     2041 cgtgagttct cccggatctc tgagataccc cagagcactc tttataagat cctttccggc
     2101 caccgggagc cgaatatcac cactttgcgc cagattgtga agaccatccg ccagctggag
     2161 ggctcgggag gggacttcat cgccatcatc gccgcccggc cagtcctaga caagatcagc
     2221 gagaagaaga tgaagatagg agagcggttg ctgacattga gagagtattc ggcaaccaac
     2281 atcgaggagg ccataattgc cgcggtgagg gcggagaggg atggagcctc ggctctggtc
     2341 tgcgctccca tcgtcagccc tacggtggaa aagctgctca ccattcctgt ggctaccata
     2401 ataccgcaag agagcgtcgt ccgggcgatt gaggttgccg ccaggaagat tgaataggat
     2461 aggatggatt gaaatgaaaa atatccctgc tgtagcagca atattgatca cttcactgct
     2521 gtttttcctt gcagtcactt gcagctccca gaatgcacct gaattccaga gcgtgagcgg
     2581 cgaatttgcc agggactgga ttgagagctt caaggccgag aatcctcagc cagt
//
LOCUS       DAOSGP010000198         2617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_37950_length_2617_cov_17.651444, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000198 DAOSGP010000000
VERSION     DAOSGP010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37950_length_2617_cov_17.651444"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PLV49_05865"
     CDS             complement(<1..1109)
                     /locus_tag="PLV49_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOG98406.1"
                     /translation="MDRLAAGLRSIAEAVLMGEIGDEAALEAAKKRLSAQLGLASLPS
                     NADILGQARAGEREALKMLIRKPTRTLSGVAVIAAMTSPARCPHGTCVPCPGGILNLS
                     PQSYTGKEPAALRACQHRFDPYDQVAARLAQLDEIGHPLDKSELIIMGGTMTSRPLGY
                     QNWFVKRCLQAMNDFPDVRESSGWQPFESVARANESALVRNIGTTFETRPDWCRPRDI
                     EEMLRLGATKVELGVQSLDEDLLVRMKRGHTVEDAAQASANLREAGLKVGFHMMPGLP
                     GSSQQRDLAVFRALFSDSRFRPDYLKIYPTLVIEGTELYRQYLRGEYTPLGDEDAAEL
                     VSRIKEILPRYVRLQRVQRDIPSPLIVAGVKKSNLR"
     gene            complement(1097..1612)
                     /locus_tag="PLV49_05870"
     CDS             complement(1097..1612)
                     /locus_tag="PLV49_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG98407.1"
                     /translation="MNLMDALKSRRSIRRYQSRPIPKDLAESLIDAAQTAPSAGNLSA
                     RRYVLVSNEDMRRLLTAAAYGQEHIEEAPLLIAVCADVKASSVRYGSRGGLYAIQDAD
                     AATMCLLLAAHDAGLGACWNGAFDDDMVREALHLEDDVLPVAIVSLGWPEESPARPER
                     PRRVGEVNWID"
     gene            complement(1685..2617)
                     /locus_tag="PLV49_05875"
     CDS             complement(1685..2617)
                     /locus_tag="PLV49_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG98408.1"
                     /translation="MIISDTHFGLDSSTLSDPSKVDRLMKEISVYGGGCDQVILLGDI
                     FDFWRVRPENALRESVPLLKRLGEEDLKIHYVVGNHDHHLVVQKQESDFMERTARGDL
                     FPVYSPSLRWRQTINGLNIDMLYPTYRIRCCHRTVLFTHGHHLAGIHIFTMQMMERIR
                     RLYGEELLPADLEMMMAYAYESIYRSSYIGEMVSFEERVWKASSLLQRITAGVFPDRH
                     IASMEAQYEAIERFIRDRNLGEIDCFIYGDTHRAGIFQRMGGPLAVNAGSFTRIPGKG
                     SRIETPDTYIILDEDGLALRQLGKAEPLFLCELL"
BASE COUNT          563 a          788 c          720 g          546 t
ORIGIN      
        1 cgaagattgg acttcttcac accagccacg atcagagggg atggtatatc cctctgcacc
       61 cgctgcaggc ggacatatcg gggaaggatc tccttgattc gtgagactag ctctgccgca
      121 tcttcatctc ccaggggagt gtactcaccc cgcaggtact gacggtatag ctctgtaccc
      181 tcgatgacca gtgtgggata aatcttgagg tagtcaggac gaaaacggct atcggagaag
      241 agcgccctga atacagccag atcccgctgt tggctggagc cgggaaggcc gggcatcata
      301 tggaatccca ccttcagtcc tgcctctcgc aggttggcac tggcctgggc agcatcctcg
      361 accgtatgcc cacgtttcat cctcaccagc aggtcctcat ccaggctctg tacacccagc
      421 tccaccttgg tcgcccccag ccgtagcatc tcttctatat cccgggggcg gcaccagtcc
      481 ggcctggtct caaaggtggt accgatgttt ctgaccagag ctgactcatt cgccctggcc
      541 accgactcga agggctgcca gccagagctc tcccgtacgt cggggaagtc gttcatggcc
      601 tgaagacagc gcttgacaaa ccagttctga tatcccaggg gtcgggaggt catcgtcccc
      661 cccatgatga tcagctccga cttgtccaga gggtggccga tctcatccag ctgggctagc
      721 cttgccgcta cctggtcata gggatcgaag cggtgctgac aggctcgaag tgccgccggc
      781 tccttgccag tgtagctttg gggtgagagg tttaagattc cccccgggca gggaacgcag
      841 gtcccatgag ggcagcgggc aggagaggtc atggcagcga tgaccgccac cccggagagg
      901 gttcgggtcg gctttctgat cagcatcttc aaagcctctc gttccccagc ccttgcctgg
      961 cccagaatat cggcattgct gggcaaggag gccaggccca gctgagcaga gagcctcttc
     1021 ttggccgcct caagagctgc ctcatccccg atctccccca tgaggaccgc ctctgcaatg
     1081 ctgcgcagcc cggcagctag tctatccaat tgacctcccc aacacgcctc gggcgctccg
     1141 gccgggccgg gctctcctcg ggccagccca gggagactat ggccacaggc aggacatcat
     1201 cctccagatg gagggcttcg cggaccatgt cgtcgtcaaa tgccccattc cagcaggccc
     1261 ccaggccggc atcatgagcg gccaggagaa ggcacatcgt tgctgcatct gcatcctgga
     1321 tggcatatag gccgccccgg ctgccataac ggacgctgga tgccttcaca tcagcgcaga
     1381 cagcgatgag aagaggagcc tcttcgatat gctcctggcc gtaggcggca gcggtcagca
     1441 gccgcctcat atcctcattg ctcaccagga catatcttct ggcggagagg ttgcccgcag
     1501 aaggtgcagt ctgggcggca tcgatcaggg attcggccag atctttgggg atgggacggg
     1561 actgatacct tcggatcgac cttctgcttt tcagggcatc catgagattc atcgattatg
     1621 aaaggcaggc cacgattata ataatcttct ccaatcacga tatgagtgga tatgatccag
     1681 aaattcataa aagctcgcat agaaataggg gctcggcctt tcccagctgc cttaaagcca
     1741 ggccatcttc atcgagaatg atgtaagtgt ctggagtctc aatgcgagag ccttttcctg
     1801 gaatccgggt gaagcttccc gcattcaccg ccaatggacc ccccatcctc tggaagatcc
     1861 ccgcccggtg ggtatcccca tagatgaagc agtcgatctc acccaggttc ctgtcgcgaa
     1921 tgaatctctc aatggcctca tactgcgcct ccatcgaggc aatatggcga tcgggaaaga
     1981 ccccggcggt gatcctctgc aaaaggctgg atgccttcca gaccctctcc tcaaaggaga
     2041 ccatctcgcc gatgtatgag gagcggtaga tgctctcata agcataggcc atcatcatct
     2101 ccaggtccgc ggggaggagc tcctctccgt aaagtcggcg aatcctctcc atcatctgca
     2161 tggtgaagat atgaatgccg gcaaggtggt ggccgtgagt gaatagcacc gtccggtggc
     2221 agcagcggat gcggtaggtg gggtagagca tatcgatgtt caggccgttt atggtctggc
     2281 gccagcgcaa agagggtgag tatacgggaa aaaggtcgcc acgcgcagtc ctctccatga
     2341 agtccgattc ctgcttttgc accaccaaat gatgatcgtg atttcccacc acatagtgga
     2401 tcttaagatc ctcctcgccc aacctcttta aaaggggaac cgactctcgc agggcattct
     2461 caggccgcac cctccagaaa tcgaatatat ctcccaataa gatgacctgg tcgcatcctc
     2521 ccccataaac cgatatctcc ttcatcaacc gatcgacctt agaaggatcg ctgagtgtgg
     2581 atgaatctag gccaaagtgc gtatccgaga tgatcac
//
LOCUS       DAOSGP010000199         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_37979_length_2616_cov_21.984772, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000199 DAOSGP010000000
VERSION     DAOSGP010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37979_length_2616_cov_21.984772"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLV49_05880"
     CDS             complement(<1..496)
                     /locus_tag="PLV49_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98409.1"
                     /translation="MGDFEWAMVNSLNSFFEREGIKAIAYRLRQSRFATQLADILVDS
                     KIAEYYLAIECKSLDARKTRSLYFKQHFSEAAGGHQIERETDFITRSGRQGILAVELR
                     YGVGKSRTAHLVPWGQIFDRYNEGGAGITLQEVQVNPPLERRGGAYEISRLDILRITG
                     YYDLA"
     gene            625..852
                     /locus_tag="PLV49_05885"
     CDS             625..852
                     /locus_tag="PLV49_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98410.1"
                     /translation="MVVGRTLWDFAFEVIRTPGFGLFLIALLIFIELILIIILLMAYA
                     GLIDIEFSYGELGLAIHHLTSHLAKRVILIC"
     gene            894..1583
                     /locus_tag="PLV49_05890"
     CDS             894..1583
                     /locus_tag="PLV49_05890"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOG98411.1"
                     /translation="MPHTASSELKSNGLGRFIRARSISDAWHRGLNLIWRQGEEISDE
                     RGTKIRELLSLQVVVENPYQEMIPEEYSWNEERLEEYAHQLLCGDNPGFEYTYGERLR
                     AWRLPGTPPLDQIDMAIQRLKESPATRRATAVTWIPPVDGERNEVPCMIVDDFKLRGG
                     RLHLAIFFRSHDFAGAYPANLYGLARLLEYAAREVGAQPGSISTTSSSAHIYEHDWDW
                     IEEMLLGRGEA"
     gene            1634..>2616
                     /locus_tag="PLV49_05895"
     CDS             1634..>2616
                     /locus_tag="PLV49_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOG98412.1"
                     /translation="MVDSLLMIKAEEIHKRIEEGKPVEYENVIIYGDLDLHNLDLPLN
                     RNKRKIVESIIKIEYSVIKGNVFFDHSAFQELVDFDGTVFSQAANFSDSFFQEDAGFS
                     QAQFHGEANFSRAHFTTEANFSRARFNDADFGRAKFHRSFHLSNARVYTLRLSDAIFE
                     EDASIHLKDLNYNRIVVRWNTIREHLPYNGSVYLTLIKNFRNLEQFEDEDDCYYQYRK
                     EKHARTTKAFPRIIDRLAWLSCGYGVRPSHTMILSLSLIILFTAIFWGAHALQPSASA
                     SPGSSISINDAFYFSSMWFLGRAPQNISIIEGFEFLTVFETLAGWLLMALFL"
BASE COUNT          596 a          737 c          673 g          610 t
ORIGIN      
        1 ctgccagatc atagtatccg gtgatcctga gtatgtccag acgggatatc tcataagctc
       61 cacctcttcg ctccagtggg ggattgacct ggacctcctg caatgttatc cccgcccccc
      121 cctcattgta gcggtcgaat atctgccccc aggggacgag atgggcagtt ctgctcttcc
      181 ccaccccata acgcagctcc accgccagta tgccctgacg gccagaacgg gtgatgaaat
      241 ccgtctccct ctctatctga tggccgcctg cggcctcgct gaagtgctgc ttgaaataaa
      301 gcgacctggt cttgcgggca tcaaggcttt tgcattcgat tgccaggtag tactcggcaa
      361 ttttagagtc caccaggata tcggccagct gggtggcaaa ccgcgactgt cggagccggt
      421 aggctatggc tttaatgcct tccctctcga agaaggagtt gagagaattg accattgccc
      481 actcaaagtc acccatcagt agagatattt caaagccctt attaaagtat cttttcccgt
      541 gtaatcactt tcgccccacc ctcattgcta cactttatag cctttggaca gtaacacttc
      601 ctgttgctct ctgtgaggat tcatatggtt gtgggaagga ctctgtggga ctttgcattt
      661 gaggtcattc gcacgccggg attcggctta tttctcatag ctttgctaat atttattgag
      721 ttaatattga taatcatatt gttaatggca tacgcaggat tgatcgatat agagttcagt
      781 tacggcgaac tgggcttggc gatccaccat ctcaccagtc atctggcgaa gagggttatc
      841 ttgatatgtt gaaaagtgga ttaacaacac atggacggag aggtggacag atcttgccgc
      901 atacggcatc atcagagctg aagagcaatg ggctgggcag gtttatacgc gcccgctcca
      961 tctccgatgc ctggcatcga ggtctgaacc tcatctggag acagggtgaa gagatctcgg
     1021 atgagagagg gaccaagata cgggagcttc tatccttgca ggtggtggtg gagaatccat
     1081 atcaagagat gattccggag gagtactcct ggaatgagga gcggctggag gagtatgccc
     1141 atcaactgct ctgcggcgac aatcccggat ttgagtatac ttatggcgag aggctgcgcg
     1201 cctggcgcct gccgggcacc cctcccctgg atcagatcga tatggccatt caacggctga
     1261 aggaatctcc tgccacccgc cgggccacgg cagtgacctg gatccccccg gtggatgggg
     1321 agaggaatga ggtcccctgc atgatcgttg atgatttcaa attacgcggc ggcaggctgc
     1381 acctggccat cttctttcgc agccatgact tcgccggggc ctatcccgcc aacctctacg
     1441 gcctggccag gctgctggaa tatgcagcac gagaagtggg tgcccagccg ggctccatct
     1501 ccaccaccag ctcctctgcc cacatctatg agcacgactg ggactggatc gaggagatgc
     1561 tcctgggaag aggcgaggca tagaccggtc gagcgaaagg catatctgca tttatcgaat
     1621 actcattacg cttatggtcg atagccttct gatgatcaag gcagaggaga tccacaaaag
     1681 gatagaagag ggcaagccgg ttgagtatga gaatgtcatc atctacggtg acctggacct
     1741 gcacaatctg gacctacctt tgaacagaaa caagcggaag atcgtagaat cgataatcaa
     1801 gatcgagtac tcggtgatca agggaaatgt gttcttcgac cactccgcct tccaggagct
     1861 ggtggatttt gacggcaccg tattctctca ggcggccaac ttctctgact ccttcttcca
     1921 ggaagacgcc ggtttctccc aggcccaatt tcacggggaa gccaacttct cccgcgctca
     1981 cttcactacc gaggccaact tctcccgagc caggttcaac gatgccgact tcggccgggc
     2041 aaaattccat cgcagctttc acctctccaa tgccagggtg tacaccctgc ggctctccga
     2101 cgccatcttc gaagaggacg ccagcatcca cctaaaggac ctgaactaca accgcattgt
     2161 ggtccgctgg aacaccatcc gcgaacatct gccctacaac ggctcagtct atctcaccct
     2221 gataaagaac ttcagaaact tggagcagtt cgaggacgag gacgactgtt actatcagta
     2281 tcgaaaggag aagcatgcac ggaccacgaa ggcatttccc agaatcatcg accgtctggc
     2341 ctggctctcc tgcggctatg gagtccgtcc atcccacacc atgatcctca gcctgtctct
     2401 gatcatcctc ttcacagcga tcttctgggg agcacatgcc ctgcaacctt ctgcttctgc
     2461 aagccccggc agcagcatat cgataaacga tgccttctac ttcagcagca tgtggtttct
     2521 tggccgggcc ccccagaaca tcagcatcat tgagggcttc gagttcctga ccgtttttga
     2581 gaccctggcc ggatggctgc taatggccct cttcct
//
LOCUS       DAOSGP010000200         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_38350_length_2600_cov_17.506876, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000200 DAOSGP010000000
VERSION     DAOSGP010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38350_length_2600_cov_17.506876"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PLV49_05900"
     CDS             <1..146
                     /locus_tag="PLV49_05900"
                     /inference="COORDINATES: protein motif:HMM:NF002316.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HOG98413.1"
                     /translation="KLKGKKIRLAKAFNQNRRVPSWVIVKTMRKVVAHPKRRHWRRSS
                     LER"
     gene            149..415
                     /locus_tag="PLV49_05905"
     CDS             149..415
                     /locus_tag="PLV49_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HOG98414.1"
                     /translation="MADTERVYTIPLGIVKCVPVYRRSNRAMAEIRNYLARHMKTPAE
                     DVKIDPGLNQVIWRRGDMKPPRRVRVRAVKFEDGGVEAEFAGER"
     gene            405..1070
                     /locus_tag="PLV49_05910"
     CDS             405..1070
                     /locus_tag="PLV49_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HOG98415.1"
                     /translation="MRDKRIKIAGSSLLGVFARCTDDIVLLPAVVGAEDLAFIEEGLH
                     VKAVCIPAGIGSVTGSLISANKSGFVVSRHAEAEDLKILREHGKVSRLPTRINAAGNV
                     ILANDSAAMVHPGLSTRACRAIEETLEVKVQKGTIGGLKTVGMAGVATNKGLLVHPRV
                     SEQEMAVLEELFELPVDVGTVNLGSPLVGSGLLANSYGYLAGDETTGPELGRIEDALD
                     FLA"
     gene            1086..1274
                     /gene="rpl18a"
                     /locus_tag="PLV49_05915"
     CDS             1086..1274
                     /gene="rpl18a"
                     /locus_tag="PLV49_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HOG98416.1"
                     /translation="MTNFEIKGRYKGGLIGKTWLPFSKVVDSQNEKNAKNMVYSLMGS
                     QHGLKRGLIKIDEVKTVE"
     gene            1267..1722
                     /gene="pfdA"
                     /locus_tag="PLV49_05920"
     CDS             1267..1722
                     /gene="pfdA"
                     /locus_tag="PLV49_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HOG98417.1"
                     /translation="MSSSPGSEEEIRRLLAAYEQYQAQADGISHQLGLSQIAAQGLES
                     ALAAVDALQEAEVGQDILVPIGSGSFIHGKLASKENVILNVGAGVSIEKRSEEAVEIL
                     KSRKNEVLEGSKKLTEVLAKIDQEMQKIQGIMQQYESRLSSGQGSEGVV"
     gene            1694..>2600
                     /locus_tag="PLV49_05925"
     CDS             1694..>2600
                     /locus_tag="PLV49_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOG98418.1"
                     /translation="MARGAKGLFSRFKEKLSGFKEALSAKVAEKVSAKGSKGAAQPEE
                     IEAEDRPALEAPDAGRKDSKAKEPKMGASSGSAKGEPLAKPSDNGEAKNKKGRFSFLQ
                     KAKTLVFEQEIILEEKDLEEPMWALEMALMESDVALPVAEEIVREVKSDLVGKKKKIG
                     SDTGQLAEDSLRNALITLLSKNHLDFDEYIRAKDKPVKILFVGVNGTGKTTSIAKVAK
                     YLMDQGYSVVLAAGDTFRAGAIEQLEVHGNNLGLKVVKHKTGGDPAAVIFDAIEYARA
                     HNKDVVLADTAGRLHTNINLMDQMRK"
BASE COUNT          691 a          601 c          817 g          491 t
ORIGIN      
        1 agaaattaaa gggcaagaag atcaggcttg ccaaagcatt caatcagaat cgtagggttc
       61 cctcctgggt catagtcaag accatgagga aggtagttgc tcaccccaag agaagacatt
      121 ggcgaagaag cagtctagag agatgacaat ggcggacaca gaaagagttt acaccatacc
      181 cctgggtatt gtgaaatgcg ttcccgttta cagaaggtcc aaccgggcca tggctgagat
      241 aaggaattac cttgcccggc atatgaagac ccccgcagag gatgtgaaga tcgatcccgg
      301 cctaaaccag gtgatctgga gaagagggga tatgaagcca ccaaggcggg taagggtgcg
      361 ggcggtcaag tttgaagacg gcggagtgga agcagagttt gccggtgaga gataagagga
      421 tcaagattgc aggtagcagt ctgctgggtg tattcgcccg ctgcacagat gatatagtac
      481 tgctgccagc agtagtcggg gcagaggatc tggcattcat agaagaaggg cttcacgtaa
      541 aggcagtatg catcccggct ggcatcggat cggtcacagg ctcgctcatt tccgccaaca
      601 agagcggatt tgtggtcagc cgccatgcgg aagctgaaga tctgaagatc cttcgagagc
      661 atggaaaggt ctccaggcta ccgaccagga tcaatgccgc aggcaatgtc atcctggcca
      721 acgatagcgc cgctatggtt catcccggcc tctccaccag agcctgtcgg gcgatagagg
      781 agacgcttga ggtcaaggtg caaaagggca ccatcggagg cctgaagacc gtgggaatgg
      841 ctggtgttgc caccaacaaa ggccttcttg tccatcccag ggtatccgaa caggaaatgg
      901 cagtgctgga ggagctattc gaactgcctg tggatgtggg cacagtcaac ctcggctcgc
      961 cactggtggg ctcaggtctg ttggcgaaca gctacggcta cctcgcagga gacgagacca
     1021 ccggcccgga gctgggccgc attgaagatg cattggattt cctggcgtaa tagaggtgaa
     1081 aattcatgac taattttgag atcaagggaa gatataaggg agggcttatt ggcaagacct
     1141 ggcttccttt cagcaaggta gtggatagcc agaacgagaa gaatgccaaa aatatggtct
     1201 actccctgat gggaagtcaa catggtctga aaagaggcct catcaagata gacgaggtga
     1261 agacagttga gtagctctcc tgggagcgag gaggagatca gaaggctcct ggctgcatat
     1321 gagcagtacc aggctcaggc agacggaatc tcccatcagc tcggcctgag ccagatcgct
     1381 gcccagggtc tggaaagtgc tctcgcagca gttgatgctc tgcaggaggc tgaggtcggt
     1441 caggatattc tggttcccat aggctctgga tcgttcatcc acggcaaact cgcctccaag
     1501 gagaatgtaa tcttgaacgt cggggcaggc gttagcatag aaaaaagatc tgaagaggcc
     1561 gtagagatcc tgaagtctcg aaagaacgag gttcttgagg gctcgaaaaa gctcacggaa
     1621 gtgcttgcca agatcgatca agagatgcag aagattcagg gcataatgca gcagtacgaa
     1681 agccgcctca gcagtggcca ggggagcgaa ggggttgttt agccggttca aggagaagct
     1741 ctccggcttt aaggaggccc tgtctgccaa ggtcgctgag aaggtttcag ccaagggctc
     1801 gaagggagca gcacagccag aggagatcga ggcagaggat aggcctgctc tggaggctcc
     1861 ggatgccggc aggaaggatt cgaaggcaaa ggagcccaaa atgggagcat cttctggatc
     1921 tgctaagggc gagcccttgg ctaagccctc ggataatgga gaggccaaaa ataagaaagg
     1981 cagattctca ttcctgcaga aggccaagac cctggtcttc gagcaggaga taatcttaga
     2041 ggagaaggat ctggaggagc ccatgtgggc attggagatg gccctcatgg agagcgatgt
     2101 cgcccttccg gtggccgagg agatcgtaag agaggtcaag tccgatctgg ttgggaagaa
     2161 gaagaagatc ggttcggata ccggccagct ggctgaggac tctcttcgaa atgcgctgat
     2221 aacccttcta tccaagaacc acctggactt cgatgagtac atccgggcga aggataagcc
     2281 ggtcaagatc ctgttcgtgg gagtgaacgg caccggcaag acgaccagca tcgccaaggt
     2341 ggccaagtat ctcatggatc agggctactc tgtggtcctt gccgcgggcg ataccttccg
     2401 ggcgggagcc attgaacaat tagaggtgca tggcaacaat ctggggctga aggtggtcaa
     2461 gcacaagacg ggcggagatc cggcagcggt gatcttcgat gccatcgagt atgcccgggc
     2521 ccataacaag gatgtagtcc tggcggacac ggctggcagg ctgcatacca acatcaacct
     2581 catggatcag atgagaaaga
//
LOCUS       DAOSGP010000201         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_38678_length_2584_cov_15.883749, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000201 DAOSGP010000000
VERSION     DAOSGP010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38678_length_2584_cov_15.883749"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..85)
                     /locus_tag="PLV49_05930"
     tRNA            complement(1..85)
                     /locus_tag="PLV49_05930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Leu,seq:gag)
     gene            complement(134..928)
                     /locus_tag="PLV49_05935"
     CDS             complement(134..928)
                     /locus_tag="PLV49_05935"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HOG98419.1"
                     /translation="MNFELLELREDRIRYLLSGVSAAFANGIRRACMSEVPILAIDEI
                     NLYDNTSVLFDEQIGLRIGEVPLVADELDLFSQPEDCQCGGTGCPGCRVDFMLSKEGP
                     GMVYSEDIQFTDPGVRPAFEKIPLVILGEGEKLVMEGYATKRVGKEHSKWQAGTLCGY
                     KNLPSITVSEACDGCGKCVEACPRDILVIEEGRAKATNILECSLCKLCVGACEAAAID
                     LKPIQDSFVVSIEGSGSVAAKDLVARAVDEIKMRALDLDSKLAELS"
     gene            complement(932..1321)
                     /locus_tag="PLV49_05940"
     CDS             complement(932..1321)
                     /locus_tag="PLV49_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOG98420.1"
                     /translation="MAEGKWGIAHIFSSFNNTIITITDQTGAETLAKISGGMVVKAAR
                     DESSPYTAMQMAMQVADAVKDKGIVGVHVKVRAPGGNQQRSPGPGAQAAIRALARAGL
                     RIGRIEDATPIPHDGTKPRGGKRGRRV"
     gene            complement(1322..1858)
                     /locus_tag="PLV49_05945"
     CDS             complement(1322..1858)
                     /locus_tag="PLV49_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOG98421.1"
                     /translation="MGYPGKSRKMYARPRTPWQAERIAGEVEVVKAYGLRNKRELWKA
                     QAVLKKYRQASRKLLAAVSLGEEPPQAQAILNRLKKLGMLKEEGDLDSILSMKVNDVL
                     ERRLQTQVYRQGLANSLKQARQFIVHGHIQVSGRRVDVPGYLVPRGEEMSIDYYMGSP
                     MASEGHPERASRTPRVEG"
     gene            complement(1880..2425)
                     /locus_tag="PLV49_05950"
     CDS             complement(1880..2425)
                     /locus_tag="PLV49_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOG98422.1"
                     /translation="MAKPTAGKEKPKKAEEKPKKGSDEELRHIVRILNTDLAGKRQVH
                     MALTGIKGVGRRCAKIFTERAGVDPNATLGLLPDAEIDKLKKVVEEDAINILPVWMVN
                     RRGDIETGKDIHIMGMDLNMTLREDLDLMKKMRSYKGLRHERGLRVRGQKTRSTGRTG
                     AIVGVSRKREGTPAAGSAAKE"
BASE COUNT          482 a          834 c          619 g          649 t
ORIGIN      
        1 tgcgggggac gggattcgaa cccgcgaacg cctgcgcgac agggccctca accctgctcc
       61 tttgacctgg cttggaaacc cccgctcaca aatatgggtt acagatcggc ttttatctta
      121 gccgatccca gctctatgat agctctgcca gctttgaatc gaggtcaaga gctctcatct
      181 tgatctcatc cactgctctt gccaccagat ccttggcggc aacagacccg ctgccctcga
      241 tggaaaccac aaaggaatcc tggatgggct taagatcaat ggctgcggcc tcacaggcac
      301 caacgcagag cttgcacaag gagcattcca gtatatttgt agctttcgct ctgccctcct
      361 ctatcaccag gatatcacga ggacaggcct caacgcactt tccgcaccca tcgcatgcct
      421 cgctcacagt gatgctgggc agattcttgt agccgcacag agttcctgcc tgccacttgg
      481 agtgctcttt gcccactctc ttggtggcat agccctccat caccagcttc tcaccctcac
      541 ccaaaatcac cagtgggatc ttctcgaagg ccggacggac tccgggatcg gtgaactgaa
      601 tgtcctcaga gtaaaccatc cccggcccct ccttggagag catgaaatcc acccgacatc
      661 cgggacatcc cgtcccgccg cactggcaat cctcaggctg ggagaagagg tccagctcgt
      721 ctgccaccag cgggacttcc cctatcctca atcctatctg ctcgtcaaat agaaccgagg
      781 tattgtcata gaggttgatc tcatctatgg ccagtatcgg aacctcgctc atgcaggccc
      841 ggcgaatgcc attggcgaaa gcggcgctaa cgccgctgag gaggtaccgg atgcggtcct
      901 ccctcagctc aagcaactcg aaattcaagc tttacaccct tcgacccctc tttcctcctc
      961 tgggcttggt cccatcgtga ggtatggggg tcgcatcttc aatcctgccg atccgcagtc
     1021 ccgcccgcgc cagagccctg atggccgcct gtgcgcccgg tcctggagac ctctgctgat
     1081 tcccgccggg agcacgtacc ttgacgtgaa ctcctacgat tcccttgtcc ttcacagcgt
     1141 cagcgacctg catggccatc tgcatggctg tgtagggaga actctcgtcc ctggctgcct
     1201 tgaccaccat tccgcccgag atcttggcca gagtctctgc tccggtctgg tcggtgatgg
     1261 tgatgatcgt gttgttaaag gacgagaata tatgggcaat gccccacttt ccttctgcca
     1321 tttatccttc caccctgggt gttctggatg ccctctccgg atgaccctcg ctggccatgg
     1381 gagagcccat gtaatagtca atgctcatct cctcgcccct gggcacgaga taaccaggaa
     1441 catccaccct cctcccggag acctgtatat ggccgtggac gatgaactgt ctggcctgct
     1501 tcagggagtt ggccagccct tgccggtaga cctgggtctg cagccttctc tccagaacgt
     1561 cgttgacctt catggataag atggaatcca gatcaccctc ctctttgagc attcccagct
     1621 tcttgaggcg gttgaggatg gcctgtgcct ggggaggctc ctctcccaaa gataccgctg
     1681 ccaagagctt tctgctggcc tgcctgtact tctttaaaac ggcctgagcc ttccacagct
     1741 ctctcttgtt tcgcagtcca taagccttga ctacctccac ctctcccgcg atcctctccg
     1801 cctgccaggg agtacgtggc ctggcataca tctttcgact cttgcctgga tatcccatct
     1861 cttaccacct tctcctttcc tattccttgg cagccgatcc tgcggctggt gttccttccc
     1921 tctttctgct gacgcccacg attgcgcccg tgcgtccggt tgacctggtc ttctgacccc
     1981 taaccctcag accccgttca tgcctgagcc ctttatagct cctcatcttc ttcatgagat
     2041 cgaggtcttc gcggagggtc atattcaggt ccatgcccat gatgtgaata tctttgccgg
     2101 tctcgatatc gcctcgccgg ttgaccatcc aaacgggcag tatattgatg gcgtcctctt
     2161 ccacgacctt cttcagctta tcgatctctg catcaggaag cagtccgagg gtggcattgg
     2221 gatccactcc cgctctctcg gtgaagatct tggcgcatct gcggcccacg cccttgatgc
     2281 ctgtcagggc catgtggacc tgtctctttc cggccagatc ggtattcaaa atgcgaacta
     2341 tatgcctgag ctcttcatca gagcccttct taggcttctc ttcagccttc ttaggcttct
     2401 cttttcctgc agttggtttt gccaagttag tcctccaggg aactttattc ctctggatca
     2461 gccccgggtc acagcccgac gccgcccgct caacacgttg tggggcgaat taatccaatg
     2521 agccaaagtg aagttatgct tctcgtataa aagacttatg aggatttggc cgtttgggat
     2581 tcca
//
LOCUS       DAOSGP010000202         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_38998_length_2570_cov_18.697018, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000202 DAOSGP010000000
VERSION     DAOSGP010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38998_length_2570_cov_18.697018"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            483..2453
                     /gene="acs"
                     /locus_tag="PLV49_05955"
     CDS             483..2453
                     /gene="acs"
                     /locus_tag="PLV49_05955"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOG98423.1"
                     /translation="MVDNKTENSAQTPNGGLFYPPEDLVENSIVMAWMKKKGMKSEKE
                     LRAWCSENYVQFWDEMARTYADWFEPYKDVVQWKAPFAKWFTGGKINIAHNALDRHAK
                     SWRRNKLAYIFVGEPMGDVRKITYYELYRDVNRFACALQKMGVKKGDRVGIYLPMIPE
                     LPVAMLACAKLGAIHVVVFSGFSAVAVRDRLEDCQPKVLITCDGSYRRGKPIATKPQA
                     DEAIAGLEYIEKVVVVKRNGQETPMQEGRDVWWDDFVAGQPATFQTLPMDSADPLFIL
                     YTAGAGGKPRGIVHTHAGASVGPAFTSSWVFDIKDTDVYWCTADIGWITGHSYIAYGP
                     LSLGATSIMYEGSPDYPDFGRWFSIIEEYGVSVIYTAPTAIRMFMNEGPEWPQKYDLS
                     TVRLMGSVGEAMNPDAFIWWRKYVGGGAAPIMDTWFQSETCSQVIAPLPITPLKPGSP
                     SFPLPGYNVRVVDENGQPSAAGATGDIIITEPWPSMLREIYRDPAQYNETYWSTFPGK
                     YFSGDKARVDEDGYIWALGRGDDVLKVAGHRISNAEVEASAEKHPAVKAAAVVGKPDR
                     VKGESIVVYAVLNPGIEGDANLQKDIKTFVRKTLGPIAIPSDVIFVEDIPRNKAGKPV
                     RPLIKARALGEKIADTSAVVNPKALDMIPLLG"
BASE COUNT          651 a          681 c          673 g          565 t
ORIGIN      
        1 gcaaaaatga atgattacaa ttatgctgct gatttgaaag cccaaaatta aggaatcgtg
       61 ataaacggct atatttcccg ccagaggcat tatctggtca ataccatcgg cactcctgga
      121 ccatatgatt ccgatttctc ccagctcgct gcgacaacaa tgctcatcca accatgacct
      181 ccgaataaac cagggattcg acgaaattgc agatagccaa tagattcgct tttctcaatc
      241 aacaagaatt ttttatggga aaaaacacca taaatctagc ttaaaagacc attaaagata
      301 aaataagtca aaagagaata tatcatcata attaggattc aacaaactta tatccttgta
      361 atgaaactta aattaattgt ctaaaaggca ttacagtgtc acaaacaatt aatcataagt
      421 agataaatca cataaagaca aataatgcct tttaaagctc taatatagta aggcgattta
      481 aaatggttga taataaaaca gaaaattctg ctcagacccc taatggcggc ctgttctatc
      541 cgcctgagga ccttgtggag aactctattg ttatggcctg gatgaagaag aagggcatga
      601 agtcggagaa agagctgaga gcctggtgct ctgagaacta tgtccagttc tgggacgaga
      661 tggccaggac ctatgccgac tggttcgagc cctacaagga tgttgtgcag tggaaagccc
      721 cctttgcaaa atggttcact ggcggcaaga tcaatatcgc ccataatgcc ctggacaggc
      781 acgccaaatc atggcggcgg aacaaactgg cctacatctt tgtgggcgag cccatgggtg
      841 acgtgcgcaa gataacctac tacgagcttt acagagatgt caatagattc gcctgtgccc
      901 tgcagaagat gggggtcaag aagggcgatc gggtgggaat atacctgccc atgattcccg
      961 agctgccggt ggccatgctc gcctgcgcaa agctgggcgc catccatgtg gtggtcttct
     1021 caggattctc cgccgtagcc gtccgcgacc ggctggagga ttgccagcca aaggtcctga
     1081 tcacctgcga tggctcttat cgcagaggaa agcccatcgc caccaagccc caggcagatg
     1141 aggccatcgc cggccttgag tacatcgaga aggtggtagt ggtaaagagg aacggccagg
     1201 agacccccat gcaggaggga agggatgtct ggtgggacga ctttgtggcc ggccagccgg
     1261 caacattcca gaccctgccc atggactctg cagatcctct cttcatcctc tacaccgccg
     1321 gcgccggagg caaacccaga ggcatagtgc atacccatgc tggagccagc gttgggcccg
     1381 ctttcacctc ttcctgggtc ttcgatatca aggataccga cgtctactgg tgcacagcag
     1441 acatcggatg gatcaccggt cacagctaca tcgcctacgg accgctctct ttgggtgcga
     1501 cgagcatcat gtacgagggc tcacccgact atcccgactt cggccgctgg ttctccatca
     1561 tcgaggagta cggggtcagt gtgatctata ctgctcccac cgccatcagg atgttcatga
     1621 atgagggacc ggaatggccg cagaaatatg acctctccac cgtccggctg atgggatcag
     1681 tgggcgaagc catgaatccc gacgccttca tctggtggag gaagtatgtg ggcggcgggg
     1741 ctgctcccat tatggacacc tggttccaga gcgagacctg ctctcaggtc atcgctcccc
     1801 tgcccataac tccgctcaag cccggctcac cctcattccc cctgcccgga tataatgtcc
     1861 gggtagtgga tgagaacggc cagccatcag ctgctggagc cacaggcgat atcatcatca
     1921 ccgagccctg gccgtccatg ctcagggaga tctatcggga tccagcccag tataatgaga
     1981 cctactggtc cactttccca ggcaaatact tctccggaga taaagcccgg gtggatgagg
     2041 atggctacat ctgggccctg ggacggggag acgatgtcct caaggtggcg ggccaccgca
     2101 tctccaatgc agaggtggaa gcctcagccg aaaagcaccc cgcagtgaaa gccgctgctg
     2161 tggtgggcaa gccggacagg gtgaagggcg agagcattgt ggtctatgcc gtcctcaacc
     2221 ccgggataga gggtgacgcc aacctgcaaa aggatatcaa gaccttcgtg cgcaagaccc
     2281 tggggccgat cgccataccc tcggatgtga tattcgttga ggatatcccc aggaacaagg
     2341 caggaaagcc tgtgcgcccc ttgatcaaag ctcgggccct tggagagaag attgccgaca
     2401 cgtccgctgt cgttaacccc aaggccctag atatgattcc gctcctcggc tgagcggaat
     2461 aactttttct ttttgtctcc tcgctatttg gtgtgggttc gtgcgctagt cacaagaatt
     2521 cgacattttt tcatgatcct ctgtgcgctc tgtgactctg aggttttatt
//
LOCUS       DAOSGP010000203         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_40111_length_2521_cov_19.252230, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000203 DAOSGP010000000
VERSION     DAOSGP010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40111_length_2521_cov_19.252230"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1089
                     /locus_tag="PLV49_05960"
     CDS             199..1089
                     /locus_tag="PLV49_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG98424.1"
                     /translation="MVIVGAGPAGLTAAMYCARGGKKTLVLGSIYGSQTAMGGLYENY
                     PGFPEGINGIELSERMLAQAQRFGAENQTELVEKIVNLGDFFRLKTENWQYESKTIIL
                     AMGASHRQIGVPGEKELTARGISYCVHCDGALYRNKAVAVIGYGNGAARAILYLANIA
                     SRVHLISPKEKLVAEPVYLERIKSLTNYAATFGAQIAEIRGEEFVESVEFNVGGTPRS
                     LKVEGVFVEMGMKPNVELAENLGLDMTSGGFVKINRLNQETSMPGVFAAGDMTGGRMQ
                     AATAVGAGASAGISALQYLG"
     gene            1093..2052
                     /locus_tag="PLV49_05965"
     CDS             1093..2052
                     /locus_tag="PLV49_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HOG98425.1"
                     /translation="MTRSEERRDRRRPVSVWRSQDLVDGRPAQALTMILRTVGCRWNR
                     CTMCGYASEGAPASAEDLIVQFESAMEKCSPEVTVVKIYTSGSFLDSEEMPAGARVEI
                     LRRLENAGIEKLIIETRPEYVNPDAVEECLSHLQTEFAMGLESANDLIREHLIRKGFL
                     FQDFVRASEMVHGQGGGVKAYLLLKPPFLTEGQAMRDAISSARAARPYADIISLNLCN
                     VQRNTVVERMWEKGEYRPPWLWSALEVLKNSPGPIICDPVGAGTRRGPHNCGQCDAAL
                     AEAIRKHDLTQDARHFDDLHCQCKEAWQKLMELEEQSFGTPLC"
     gene            2103..>2521
                     /locus_tag="PLV49_05970"
     CDS             2103..>2521
                     /locus_tag="PLV49_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG98426.1"
                     /translation="MFEKVLIATDFSKHAKKVIECIGEMPGLKEVLLLNVISRDPLAR
                     VWDPVSEIKDTEKKLEEEKKAIGAPGVTVKVKAVSVLEGEVASAINKVAMEENVSLVA
                     MGARGRSLIMSTLLGSSSRNVLRFGRTHLLIMRYKTL"
BASE COUNT          595 a          644 c          793 g          489 t
ORIGIN      
        1 gaacccgagt ttaggcgttg gcaacgccta gtgataaccg ctacactatc ggcgcggcgc
       61 ttgctggaag catccccatc aacaaccttc tagtctaaaa acattgcggg cagatagtac
      121 cctggaagcc caccatatta ctgcacactt tatctacctt gaaagggtgc tgatttatgg
      181 tgaattcgct tgtatgatgt ggtcatagta ggagcaggcc cggcagggct gaccgctgcc
      241 atgtactgcg ccagaggagg gaagaagacg ctggttttgg ggagcattta cggctctcag
      301 accgccatgg gtggcctcta tgagaactat cccggctttc cggagggcat taatggcatt
      361 gagctctcgg agaggatgct ggcccaggcc cagaggtttg gagcggagaa ccagacggaa
      421 ctggtggaga agatcgtgaa cctgggagac ttcttccgcc tgaagactga gaactggcag
      481 tatgagtcca agactatcat cctggccatg ggggccagcc atcgccagat aggggttcca
      541 ggcgaaaaag agctgacggc cagaggaatc tcctattgcg tccattgcga tggtgcccta
      601 tataggaaca aggcagtggc ggtgattggc tacggcaatg gcgcagccag agcaatccta
      661 tatctggcca atatcgcgag ccgagtccac ctcatatcac ccaaagagaa gctggttgca
      721 gagccggtgt atctggaaag gatcaagtcg ctcaccaact atgccgccac ctttggcgcg
      781 cagattgccg agatccgagg ggaagagttc gtggaaagcg tggagttcaa tgtgggggga
      841 accccccgtt ccctgaaggt ggaaggggtc ttcgtggaga tgggcatgaa gcccaatgtt
      901 gagctggcag agaaccttgg cctggatatg acctctgggg gctttgtgaa gatcaaccgg
      961 ctcaaccagg agacgagcat gcccggtgtc ttcgccgccg gggacatgac cggcggacgg
     1021 atgcaggcgg caactgctgt gggtgcgggc gcctccgccg gcatcagcgc tctgcagtat
     1081 ctcgggtaga cgatgacaag atctgaggaa agaagggacc gcagaaggcc ggtcagcgta
     1141 tggcgatctc aggatctggt ggatggacgc cctgcccagg ctctgaccat gatcctgcgc
     1201 acggtgggat gcagatggaa ccgctgcacc atgtgcggct atgccagcga gggcgcaccc
     1261 gcctctgcgg aagacctgat cgtccagttc gagagcgcca tggaaaagtg ctctcctgag
     1321 gtgacagtgg tcaagatcta caccagcggc agcttcctgg actcagagga gatgccagca
     1381 ggggccaggg tcgagatact gcgccgcctg gagaatgcgg ggatcgagaa gctgatcatt
     1441 gagacccggc cggagtacgt gaaccctgat gcggtggagg agtgcctctc ccacctgcag
     1501 accgagttcg ccatgggcct tgagagcgca aacgacctga tcagagagca cctcatccgc
     1561 aagggctttc tcttccagga cttcgtccgc gcctcggaga tggtgcacgg ccagggagga
     1621 ggggttaagg cctatctgct cctgaagccg ccctttctca ccgaaggtca ggctatgcgt
     1681 gatgccatat cctccgctcg ggcagcacgc ccttatgcag acatcatctc cctgaacctg
     1741 tgcaatgtgc aacgcaatac agtggtggag aggatgtggg agaagggcga gtatcggcca
     1801 ccatggctct ggtccgccct tgaggtgctg aaaaactccc caggccctat catctgcgac
     1861 ccggtgggtg caggcaccag gcgggggccc cataactgcg gccagtgcga tgccgccctg
     1921 gcggaggcga tcaggaagca cgatcttacc caggatgcga ggcactttga tgatctgcac
     1981 tgccagtgca aggaagcatg gcagaagctg atggagctgg aagagcaatc cttcggcacc
     2041 cctctctgct gaaatagatt gaaatatcaa gaaatgaaac tagttcataa ggtgagctag
     2101 aaatgtttga aaaggtattg attgccacag atttctccaa gcatgccaag aaagtgatcg
     2161 aatgcatcgg cgaaatgccg ggattgaaag aggtactact tctcaatgtc atttctagag
     2221 atccgctcgc ccgtgtctgg gatccggtat cggagataaa ggataccgaa aagaagctgg
     2281 aagaggagaa gaaggccata ggagctccag gggtaacggt taaggtgaaa gcggtatccg
     2341 ttctggaggg cgaggtggca agcgccatca ataaagtggc aatggaagag aacgtctctc
     2401 tggtggccat gggagcgcgg ggaaggagcc tgatcatgag cactctgctt ggaagctctt
     2461 cccggaatgt gcttcgcttc ggcaggacgc acctcttgat catgcgctac aagaccctgg
     2521 a
//
LOCUS       DAOSGP010000204         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_40468_length_2507_cov_20.813622, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000204 DAOSGP010000000
VERSION     DAOSGP010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40468_length_2507_cov_20.813622"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1200
                     /locus_tag="PLV49_05975"
     CDS             139..1200
                     /locus_tag="PLV49_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOG98427.1"
                     /translation="MQLKLTSKERLVLYGLARYPGFSDIDLASNIGVDRSTIFKSKRK
                     FRDWKLIKLLNVPSGEAVGAEILTSVFVKYNPTAPYEVRKESESHKRWINYPNCVSHT
                     ATDTDSVSTFYSRSLTDFRVNFDPIIDEYYRKDFVEDIHYYHYPFQVSGYSSDAALAV
                     DGLFELERADLPSGMIPPRPVLTEDSRLTEKDKLTLYAFVKYPMLSDLELSRRTGISR
                     PTISGKRTKFFQSGLLTREAYIDWQKICCELMSFYHVPIRHGHDREDLAKVYQAFRRV
                     GAPLFTYMQPGEIFGAFLSTGYPELKSRLDQELRNLSSQGLITERPMLVIMPLNEIKS
                     TKIDYAPMVADMLGIGREI"
     gene            1413..2039
                     /locus_tag="PLV49_05980"
     CDS             1413..2039
                     /locus_tag="PLV49_05980"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOG98428.1"
                     /translation="MIVLPCRHEAKAALPWFGGHFTGILEVGEGKSRLSAAAPSGLRS
                     FLHNISAIVSLSMPGYMVSAEATHHAPVDVRTPSFFAEIGSTKEQWVDLKAGEAVARA
                     ILALEPVELPVFLGFGGGHYVQRQTELIFNSRIAFGHMFSSYQVPDLDLEAIEIARES
                     SNATYAYIDRKSLRSAERKRLERMVEEIGLPMLKAQEIRARFSPSDAI"
     gene            2290..>2507
                     /locus_tag="PLV49_05985"
     CDS             2290..>2507
                     /locus_tag="PLV49_05985"
                     /inference="COORDINATES: protein motif:HMM:NF016098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98429.1"
                     /translation="MKMGEKSEFAAKGSIAALQEKAMDIIASRPEGIYQSDLRRLLEI
                     DSSKCSKVVARLQGSELIRRESVPASST"
BASE COUNT          637 a          593 c          655 g          622 t
ORIGIN      
        1 aaagtgcaga ccatgagcct gcagattaaa ttatggcaaa attgacctgc tgataccatg
       61 taaaatgaag gacgtatgta ttctatactc taagaataag caatctataa atacttatga
      121 aatgtatatg atatgcgaat gcagctaaaa ttaacatcaa aggagaggtt agttctttac
      181 ggtcttgcca gatatccggg atttagcgat attgatctcg cctccaacat aggcgtggat
      241 cgatcaacca tattcaagag caaaaggaag ttcagagact ggaaactcat taagctattg
      301 aatgtgcctt ccggagaagc tgtgggtgcc gaaatcctga cctcagtgtt cgtcaagtac
      361 aatccgactg ccccatacga agtgaggaag gagtctgagt cccacaagag gtggataaat
      421 tatcctaact gcgtatctca tactgcaaca gacaccgatt ccgtatctac attttactcc
      481 cggagcctga cggattttcg ggtgaacttc gatccaatca tcgatgagta ttacaggaaa
      541 gatttcgttg aggatattca ttattaccac tatccattcc aggtctcagg ctactcatct
      601 gatgccgccc tggcagttga tggcctcttc gagctggaaa gagctgatct tcccagcggg
      661 atgatccctc cccgtcccgt actgactgag gactcacggc tgacggagaa ggacaagctg
      721 accctatatg cctttgtgaa gtatcccatg ctctccgacc ttgagctctc cagaaggacg
      781 ggcatatcca ggcctacgat ctccggaaag cggaccaagt tctttcagag cggcctcttg
      841 acccgggaag cctatatcga ctggcagaag atctgctgcg agctgatgtc cttctatcat
      901 gtacccatca ggcacggcca tgaccgggaa gatctggcca aggtctatca ggccttccgc
      961 cgggttggag cgccgctctt tacctatatg caaccgggcg agatattcgg ggcgttcctc
     1021 tccactggct atccagagct taagagccgc ctggatcagg agctgcgcaa cctctcttcc
     1081 caggggctga tcactgaaag gcctatgctg gtcatcatgc ctctcaatga gataaagtcc
     1141 accaagatag actatgcgcc aatggtagcg gacatgctgg ggatcggcag ggagatctga
     1201 gaattcggca gcttggtcag atcgctgtag tctgctctcg ggcagatccg gcaagcttca
     1261 atatcttcga gcgtctgctt gagctgcagg cttggcagga gtgcaaagga taccgatcca
     1321 gcggaaactt ccgtctcctg gttcatgatg ataagcagac cgcccttatt ggtctggacg
     1381 atcggctggc agatctggat ctttatccag acatgatcgt cctcccctgc aggcatgagg
     1441 ccaaggcagc attgccctgg ttcggagggc attttactgg cattttggag gtgggagagg
     1501 gcaagagccg tctttccgct gccgctccct ctggcttgag gtcatttctg cacaatatct
     1561 cagctatcgt ctccctgtcg atgccggggt atatggtctc agctgaggcc actcatcatg
     1621 ctcctgtgga tgtaaggaca ccatccttct tcgccgagat cggcagcaca aaggagcagt
     1681 gggtcgatct gaaggcgggg gaggcggtgg ccagggcgat tttggccctt gaaccggtgg
     1741 agttgcccgt gtttctggga tttggaggag gtcattatgt gcagaggcag actgagctga
     1801 tcttcaactc tcgcatagct tttggccata tgttctccag ctatcaggtc ccggatctgg
     1861 atctagaggc gattgaaatt gctcgggaaa gctcgaatgc cacttatgcc tatatcgacc
     1921 gcaagtcgct gcgatccgcg gaacgaaaga gacttgagag aatggttgaa gagatcggac
     1981 tgcctatgct caaagcgcag gagattcggg cccggttttc cccgagcgat gcgatataat
     2041 aatattagat tttcccgccg ccaacattac gatcctttgg cagaagctga atatctaata
     2101 aaatatttta atattgatcg attttctgca ataaatcccg ggattctatg gaattttctc
     2161 ggaatatgag gttatatctg ttatttttag acaaaataag gggcaaatgc aaaagagttt
     2221 tatatcaaga gaattcatta cagtatatca aaaataatta agtttgggct ctatctgacc
     2281 aaagggagta tgaaaatggg ggagaagtca gaatttgccg ctaagggcag cattgctgct
     2341 ctgcaggaga aggcgatgga tatcatagcc tcgcggccag agggaattta tcaatccgat
     2401 ctgaggaggc tcctggagat cgacagcagc aaatgctcaa aggtggttgc caggctgcag
     2461 ggctcagaat tgatccgcag agagagcgtt cctgccagca gcaccta
//
LOCUS       DAOSGP010000205         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_40736_length_2496_cov_21.441213, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000205 DAOSGP010000000
VERSION     DAOSGP010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40736_length_2496_cov_21.441213"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PLV49_05990"
     CDS             complement(<1..950)
                     /locus_tag="PLV49_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG98430.1"
                     /translation="MERSHAAIALPELDLKDEYARFLKRKFLVLVAALLGIVALAGVA
                     VTLGSAHITPFQVYSCILARFFPDYFQTTDFLDTIVWKLRLHRVILAIFAGMGLAIAG
                     AAMQGILKNPLASPFTLGIASAASFGASLAIILGAGFIGGEYLIVGNAFIFTIMASLA
                     VYGLAKYRGISPETMVLAGIAIMYLFSAMTSFLQYVGHAEQVHEVVFWMMGSLGRSSW
                     DKVGIIALVTVLCSLILMRLSWDINTLGAGDETAKSLGVNVELVRVSGMMLAALITAS
                     VICFTGTIGFIGLVAPHITRMVVSGDHRFLLPASGLFGALL"
     gene            complement(973..1425)
                     /locus_tag="PLV49_05995"
     CDS             complement(973..1425)
                     /locus_tag="PLV49_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOG98431.1"
                     /translation="MLLLVNLAGADVLINEMLANPATDWNGNGQVGGMDDEFIELYNS
                     GNESVDVSGYSLKDKVFRSTPTEYSIPNGTVIGPGEFLVIFVSESGVYQGNEGDAIML
                     YDPTGDLVDEKEYDAATEDVSFARIPDGGEWRNSTAPSPGKPNQEAAS"
     gene            complement(1486..2409)
                     /locus_tag="PLV49_06000"
     CDS             complement(1486..2409)
                     /locus_tag="PLV49_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HOG98432.1"
                     /translation="MRKIALGLAALIAVAFVVPSYGAMDRPAKEDPAILLVVFGTSYP
                     EAQAAYENIERVYREEFPQAEVRIAFTSDYIRRKLLDRDNVTIDNPLTALAHLNDEGY
                     TDVVVQSLHVIPGEEFHDLANIVESVRGIEGKFGFRNLVLGEPLLMNLEDYHNVSRAL
                     ASQFEQNTTGTERTPHSSPRDAGQMAVIYMGHGTEHSANSAYSQMANILAEDHENVFL
                     GTVEGYPGYDEVLASLNESGVKKARLIPLMVVAGDHALNDLTGNESESWKSMLEKEGF
                     EIDYNLLGMGENDGIVEIFVGHTKEAFAKFK"
BASE COUNT          605 a          701 c          612 g          578 t
ORIGIN      
        1 agcagggcac cgaaaagccc tgatgccgga agcaggaacc tgtgatctcc cgacacaacc
       61 atcctggtga tatgaggagc caccaggccg atgaagccga tggtgccggt aaagcagatc
      121 acgctggcgg tgatcagagc ggccagcatc attcccgaga cccttaccag ctctacattg
      181 acccccaggc tcttggcggt ctcgtctccg gctcccaggg tgttgatgtc ccaggagagc
      241 cgcatcaaaa tcaacgagca gagcaccgtc accagggcga ttatgcccac cttgtcccag
      301 gaggagcgac cgaggctgcc catcatccag aagaccacct cgtgaacctg ctctgcatgg
      361 cccacatact gcagaaatga ggtcatggca ctgaaaaggt acatgatggc gatgccagcc
      421 aggaccatgg tctccgggct aatcccacgg tacttggcca ggccgtagac cgctaaagag
      481 gccattatgg tgaagatgaa ggcattgccg acgatcagat actcgccgcc gatgaaacct
      541 gctccgagaa ttatcgccag cgatgcccca aaggaggcag cagaagcgat tcccagagta
      601 aaaggactgg ccagaggatt tttgagaatg ccctgcatgg ctgctcctgc aatggccagc
      661 cccattcctg caaaaatggc caagatcacc cggtgcagcc tcagcttcca gacgatggta
      721 tccaagaagt ctgtggtttg aaagtagtca ggaaagaacc tggccagtat gcaggagtaa
      781 acctgaaacg gagtgatatg ggccgagcca agcgtcactg cgactcctgc cagagctact
      841 attcccagca gcgcagcgac caagaccagg aatttgcgtt tcaggaatcg agcgtactca
      901 tccttaagat ccagttccgg caaggctatg gcagcatgag atctctccat ttaaagcacc
      961 acatcttgct ctctaggaag ctgcctcttg gtttggcttg ccaggcgatg gagcggtgct
     1021 gttgcgccat tcccctccat ccgggatccg ggcaaaagag acatcctctg ttgcggcatc
     1081 atactctttt tcgtccacca ggtctccagt gggatcatac agcattatag cgtcaccctc
     1141 atttccctga tatacgccgc tctcagagac gaatatgacc aggaattctc cagggcctat
     1201 cacagtccca ttgggaatcg agtactctgt gggcgtcgac cggaatacct tatctttaag
     1261 agagtatccg ctgacatcca cggactcgtt tccggaattg taaagctcta tgaattcatc
     1321 atccattccc ccaacctgtc cgttgccgtt ccaatccgtg gctggattgg ccagcatctc
     1381 attgatcagc acatcagcac cagccaggtt taccagcagg agcatcgata gcagatagat
     1441 tgaatatctc aatttaaggc ctcctaaaat atattttatg attaattatt taaatttggc
     1501 aaaggcttcc ttggtatgcc caacgaatat ctcgacaatg ccgtcgttct cgcccattcc
     1561 caagagattg tagtcgatct cgaatccttc cttctccagc atgctcttcc aggattcgga
     1621 ctcattgccg gtgagatcgt ttaaagcgtg atctcctgcc acgaccatga gggggatcaa
     1681 tctcgccttc ttcacgcccg attcattcag gctggccagc acctcatcgt agcccggata
     1741 tccctccact gtgcccagga agacgttctc gtgatcctct gccaggatgt tggccatctg
     1801 ggagtaggcg ctgtttgccg aatgctcggt tccatgcccc atgtagatca ctgccatctg
     1861 cccggcatct ctggggctgg aatgaggagt tctctccgtt ccagtggtat tctgctcaaa
     1921 ctggctggca agagctctgg agacgttgtg gtaatcctct aaattcatga gcaggggctc
     1981 gccgaggacc agattccgaa agccgaactt tccctcaatc ccccttacgc tctctacgat
     2041 gttggccagg tcgtggaact cctcgccagg tatcacatgc agcgactgga ctacaacatc
     2101 agtataacct tcatcgttga gatgagcaag ggcggtgaga gggttatcta tggtgacatt
     2161 atccctatcc agcagctttc tccggatata gtctgaggtg aaagcgatcc ttacctccgc
     2221 ctggggaaat tcctcccgat agactctctc tatgttctcg taggctgcct gggcctcagg
     2281 atagctggtg ccgaatacca ccagaagtat ggccggatct tccttggcag gcctgtccat
     2341 ggccccgtat gaaggaacca caaacgccac ggcgatcaat gccgccagtc ctaatgctat
     2401 ctttctcata tgacgcacct attcaaataa agtttattta aaacaagaga attggttata
     2461 acataaaaga gttgcgctga attgatctga tataaa
//
LOCUS       DAOSGP010000206         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_41255_length_2475_cov_13.408678, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000206 DAOSGP010000000
VERSION     DAOSGP010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41255_length_2475_cov_13.408678"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLV49_06005"
     CDS             complement(<1..645)
                     /locus_tag="PLV49_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOG98433.1"
                     /translation="MTENNENCETCTHQGTCPSQNSDQAAACVDNLARKIKHKIIIAS
                     GKGGVGKSTVSVNLARALQLEGFKVGILDADITGPNIPKLLGIEDQSLVMGDEGIRPA
                     DAGGIKAASMALVLSSTDSPVVWRGPMKMAAIKQFIQDVDWGELDFLIIDLPPGTSDE
                     PLSVVQLIPALSGAIIVTTPQEVSLLDSRKAVNMVRGMKLPVIGIIENMAGLMCP"
     gene            complement(674..1582)
                     /locus_tag="PLV49_06010"
     CDS             complement(674..1582)
                     /locus_tag="PLV49_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98434.1"
                     /translation="MGYLFGPVLSRRLGLSMGVDLLRHKTCNLDCIYCELGRTDCLTC
                     RRGRFVPPQNVLREIREKRNEPFDYLTFAGSGEPTLSQDLGRVVAFAKETVSRPVAVI
                     TNSTLLTSPAVRKEVAAADIVLPSLDAASPAAFQAINRPDPGLKIEEIVQGLKEFRRE
                     YSGEIWLEVMLVAGINDHEADLIARAAESTEPDRIQLNTVVRTPAEPVEPLSEEEMVR
                     MLEIFPGAELIPDWDWRVPVDIRNRILDSLCRSPSTLEEICRLHDLTDSDAIKYCKIL
                     EQDGLITRRIEGGKLCFQGSKSRGRG"
     gene            1697..>2475
                     /locus_tag="PLV49_06015"
     CDS             1697..>2475
                     /locus_tag="PLV49_06015"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG98435.1"
                     /translation="MDKAHELIKKLGQSDDYFERQKAAWALANLGEEAVDAVAEALEK
                     GEFSDLRYKSAWILGKTGSDRALKPLCYALLSDPDHVVREWSAAALEALGNQEAVQPL
                     VLAMKRDSSRDVRLRAAMALRALEAAEAFRELLSYSDPEVRGMATTGLAKIGYAQSLP
                     DVARLIEDEDAEVRRRAAAYMGEIASEEAIESLAIALKDSEADVRCEALKSLGKIKGE
                     GAADLALAALLDKEWKVRYTAVTALGEIGHDKALEALIEVM"
BASE COUNT          545 a          700 c          679 g          551 t
ORIGIN      
        1 gggacacata aggcccgcca tgttctcgat aattccaata acaggaagct tcatccccct
       61 gaccatgttc actgctttgc gggagtcaag cagggagacc tcctgagggg tggtgacaat
      121 tatcgctcca gagagagcgg gaatgagctg caccacgctt aaaggttcgt cactggtgcc
      181 tggtggaagg tctatgatca gaaaatcgag ctctccccag tctacatcct ggatgaactg
      241 cttgatggca gccatcttca taggacccct ccacaccact ggggagtcgg tgctggagag
      301 aactagggcc atggaggccg ccttgatacc gccggcatcg gccggtctta tcccctcatc
      361 tcccatcacc agactctgat cctcaattcc caggagcttt ggtatattcg ggccggtgat
      421 atcggcgtcc agaatgccaa ccttgaatcc ctccagctgc agagcacggg ccaggttgac
      481 agatactgtg ctcttgccca ctccaccctt gccgctggcg atgatgatct tatgttttat
      541 ctttctcgcc aggttatcta cgcatgcggc tgcttggtcg ctattctggc tggggcaggt
      601 gccctgatga gtgcatgtct cgcaattttc attattctcc gtcatcgaaa accatccatt
      661 tgaggatttt tagttatcct ctgccacggc ttttgcttcc ctgaaaacag agctttcctc
      721 cttcgatcct tctggtgatc agaccatctt gctccaggat cttgcagtat ttgatggcat
      781 cgctatcagt aagatcatga aggcggcaga tctcctccag ggtagagggc gagcggcata
      841 agctgtccag gattctgttg cgaatatcaa ccgggaccct ccagtcccag tcgggaatca
      901 gctcagctcc tgggaagatc tccagcatcc gaaccatctc ctcctcactc aggggctcta
      961 ccggctcagc cggagtcctg acaacggtgt tcagctgaat tctgtccggc tcagtcgatt
     1021 ccgccgctcg agcgatgaga tctgcttcat ggtcgttgat cccagccacc agcatcacct
     1081 caagccagat ctctcccgag tactcccggc gaaactcctt gaggccctga actatctcct
     1141 cgatcttcag gcccggatca ggccggttta tcgcctggaa ggcagcggga gaagcggcat
     1201 caagggaggg aaggacgata tccgctgctg caacctcctt tcgaactgca gggctggtaa
     1261 gaagggtgct gtttgtgatc accgccactg gacgggagac cgtctctttg gcgaaggcca
     1321 ccacccttcc cagatcctgg ctcaacgtcg gctctcccga accggcaaac gtcaggtagt
     1381 cgaagggctc attcctcttc tctctgatct ccctgagcac attttgcgga ggcacaaacc
     1441 gtccccgtct gcaggtcagg caatcggtcc ggcccagctc gcagtagatg cagtccagat
     1501 tgcaggtctt gtgcctgagc aggtccaccc ccatggatag ccccaagcgg cgggagagca
     1561 caggtccgaa tagatatccc atgttttctg ttcctgtcca atgtctgccg aagcagccct
     1621 ttaacgctta aacatttgca tgcaattgaa ttctatttgc acaaagctta atagactgcc
     1681 atgcccatga atcggcatgg acaaggctca tgaactcata aagaagctcg gccagagcga
     1741 tgactatttt gaacgtcaga aggcggcatg ggccctggca aacctgggcg aggaagcggt
     1801 cgatgccgta gctgaagccc tggaaaaagg cgaattctcc gacctgcgct acaagtccgc
     1861 ctggatcctg ggcaagacgg gatccgacag ggcgttaaaa cccctctgtt atgctcttct
     1921 cagcgatcca gatcatgtag ttcgcgaatg gagcgcagcc gcattggagg ctcttggcaa
     1981 tcaggaggca gttcagcctc tggttttagc catgaagaga gacagctcca gggatgtgcg
     2041 cttgagagcg gctatggccc tccgcgcttt agaggcagca gaggctttca gagagctact
     2101 gagctattct gatcccgagg tccgggggat ggctaccacc ggcctcgcga agatcggata
     2161 tgctcaatcc ctgccggatg tggccaggct aattgaagac gaggacgcgg aggtgaggcg
     2221 aagggcggca gcctacatgg gagagatcgc cagcgaagaa gctatcgaat ctctcgctat
     2281 tgccctgaag gacagtgaag cggatgtacg ctgcgaggcc ttgaagtccc tgggaaagat
     2341 aaaaggggag ggcgccgcag atcttgctct tgccgctctg ctggacaaag aatggaaggt
     2401 gcgctatacc gccgtgaccg ccctgggaga gataggccac gacaaagccc tcgaagccct
     2461 gatagaggta atgtt
//
LOCUS       DAOSGP010000207         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_41890_length_2448_cov_19.451316, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000207 DAOSGP010000000
VERSION     DAOSGP010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41890_length_2448_cov_19.451316"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /gene="radA"
                     /locus_tag="PLV49_06020"
     CDS             complement(<1..593)
                     /gene="radA"
                     /locus_tag="PLV49_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HOG98436.1"
                     /translation="MAIKLLEDLPGVGPATAEKLREAGFNSIEAIAVASPGELVTAAE
                     VGEATAAKIIAGAREAADVGGFETGDRILERRKQVGKVTTSCKSFDELLGGGMETQAI
                     VELYGEFGCGKTQVAHQLAVNIQLPVEMGGLNGSVIIIDTENTFRPERIDQMVKGLPP
                     APDGRIWETEDFLKNINVARAFNSNHQILLAESAMDLA"
     gene            complement(640..1755)
                     /locus_tag="PLV49_06025"
     CDS             complement(640..1755)
                     /locus_tag="PLV49_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOG98437.1"
                     /translation="MDSELEEIYLQVADSISPEEFEERVKEKVALMAGLCDSRTAAML
                     VARDLGSPDLLTKIGSIRPEMGNVTFTGRVIAVSPVREFQRSDGSLGRVANITLADET
                     GSVRVALWDETAELIKSGDLAVDQCLKVRGLAKEGFAGTEVSLGRGGGFEEIDQDIHP
                     RVAPYKIGELKRDMSDVSLLAVVVDPGEIREFERRDGGKGQVRGVTLGDETGKIRLTL
                     WDEQAQMPLLKGETLEIVGGSVRERYGLLEIQTGGSTAIRKSTKKLDFSERMTPIAEL
                     KEGMLSSVSGFVSGLGEVREFQRDDGRVGRVANIYISDDSGRIRAALWGEHVDRLVEV
                     DLGYRAEIIDAQVKSGWNEGLELSCGWRTRITFAPPE"
     gene            complement(1762..>2448)
                     /locus_tag="PLV49_06030"
     CDS             complement(1762..>2448)
                     /locus_tag="PLV49_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG98438.1"
                     /translation="RRTLGIMYQEFALYAGETIIDQIAYQMGIKSTDVIEYARSVAEE
                     MGISEKVLDVIYAMADMSEEDAKSALESLELTRDIFKDLFPRFPNTEAQKFAEPIFDL
                     LDLDRSVMWKLPRQLSGGELVRASLAILLAAQPEVMILDEPFGDIDPITLREVSNAIK
                     KINSEFGTTIILVSHHVDFVKEVSHRAILIENGALIEDGDPIEISNAFLSRCNAPYLR
                     STQERYAIHG"
BASE COUNT          496 a          802 c          585 g          565 t
ORIGIN      
        1 gccaggtcca tggcgctctc cgccagcagg atctggtgat tggagttgaa ggccctggcc
       61 acgttgatgt tctttaagaa atcctcagtc tcccagatcc ggccgtcggg tgcaggcggc
      121 agccccttga ccatctggtc gatgcgctcc ggccggaatg tgttctcagt atcgattatg
      181 atgacggagc cattcagccc gcccatctcc accggcagct ggatattcac tgccagctgg
      241 tgggcgacct gggtcttgcc gcatccgaac tctccgtaga gctccacaat ggcctgagtc
      301 tccatacctc cgcccagcag ctcatcgaag ctcttgcagc ttgtagtgac cttacccacc
      361 tgctttctgc gctccaggat gcggtcaccg gtctcaaagc ctcctacgtc tgccgcttct
      421 cgggccccgg ctattatctt ggccgcagtg gcctcaccaa cctctgccgc agtaaccagc
      481 tctccaggag aggcaacggc aatggcttcg attgagttga atcccgcctc gcgcagcttt
      541 tcggcagtgg ccggacccac gcccggcagg tcttccagta gttttattgc catcttcaca
      601 gttcctctgg taggtgttct cataactcac ttaacttcct tactcaggcg gggcgaaagt
      661 aatcctggtg cgccatccgc aggagagctc cagtccttca ttccagccgc tcttgacctg
      721 cgcatcgata atctccgccc tgtatccaag atcaacctcc accagtcgat cgacatgctc
      781 tccccataga gctgcccgga tccggccgga atcatcggag atgtagatat ttgccaccct
      841 gcccactctg ccatcatccc tctgaaactc ccgcacctcc cccaggccgg atacaaagcc
      901 ggaaacgcta gagagcattc cctctttcag ctccgctatt ggtgtcatcc tctctgagaa
      961 gtccagcttc ttagtgctct tgcgaatagc ggtgctccca ccggtctgga tctcaagcag
     1021 gccgtacctc tcccggactg agccgcctac gatctcaagc gtctccccct taagaagagg
     1081 catctgggcc tgctcatccc agagggtgag ccggatcttg ccggtctcat ctcccagcgt
     1141 cacccctcga acctggccct tgcccccatc tcggcgttca aactccctga tctctcctgg
     1201 atccacaacc accgccagaa ggctgacatc actcatatcc ctcttcagct ctccaatctt
     1261 gtaaggcgcg acgcggggat gaatatcctg atctatctcc tcgaatccgc cgccacggcc
     1321 caggctgacc tcagtccccg caaatccctc tttggccagt cccctcacct tgaggcactg
     1381 gtccacagcg aggtctcctg acttgatcag ctctgctgtc tcgtcccaga gggcgacacg
     1441 caccgacccg gtctcgtccg ccagggtgat gtttgccacc ctgccaagag atccgtctga
     1501 tcgctgaaac tcccgcacag gagatacggc gatcaccctg ccggtgaatg tgacgttccc
     1561 catctcaggc cggatgctcc cgatcttggt cagaagatca gggctgccca agtccctggc
     1621 caccagcatg gccgcggtcc gggagtcgca taagccggcc ataagggcca ccttctcctt
     1681 caccctctcc tcgaattctt caggtgatat gctgtctgca acctgaaggt aaatctcctc
     1741 cagctctgaa tccatcaacg atcatccatg aatagcataa cgctcctgcg tacttctcag
     1801 gtagggggca ttgcagcggg agaggaaggc attagagatc tcaatgggat cgccgtcttc
     1861 aattagcgct ccattctcta tcagtatggc ccggtgagat acctccttga cgaaatccac
     1921 atgatgactc accaggatga tggtcgtccc aaactccgag ttgatcttct ttatggcatt
     1981 ggagacctcc cgcaatgtga tgggatcgat atctccaaac ggctcgtcca gaatcataac
     2041 ctctggctgg gcggcaagga gaatggccag ggaggctctc accagctccc cgccggacag
     2101 ctgcctggga agcttccaca tgacacttct gtccaaatcc agcaggtcga atatgggctc
     2161 ggcgaacttc tgggcctcag tgtttggaaa gcgggggaat agatccttga atatatccct
     2221 tgtaagctcc agagactcta gagctgactt tgcatcctct tctgacatat ccgccatggc
     2281 atagataaca tccagcacct tttcgcttat gcccatctcc tcggcaacgg aacgggcata
     2341 ttcaatcaca tcagtgcttt taatgcccat ctgatatgcg atctgatcga tgatggtctc
     2401 cccggcataa agggcgaact cctgatacat gatccctaaa gttcgacg
//
LOCUS       DAOSGP010000208         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_42038_length_2442_cov_14.989108, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000208 DAOSGP010000000
VERSION     DAOSGP010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42038_length_2442_cov_14.989108"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..>2442
                     /gene="ppsA"
                     /locus_tag="PLV49_06035"
     CDS             212..>2442
                     /gene="ppsA"
                     /locus_tag="PLV49_06035"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOG98439.1"
                     /translation="MEAVVWLEDVGKEDLSIVGGKGASLGEMISIGVPVPGGFAVTAQ
                     AFRDFINRAGIADKLFEALNVDVNQETELHRAEQTAKKLIMDAKVPKDIEEAIKTRYE
                     ELCKREGKQVFVAVRSSATAEDLPDASFAGQQETYLNMRGAEDVFNAVRKCWASLYGA
                     RAIFYRVEQGFEHNKVNLSAIVQLMVDSEKAGVMFSSQPSTGEPLVVIEAAWGLGESV
                     VSGSVSPDNYVVDRKTKKIVNKYIATKEIMITRDPKTHKTTTTKVPAKKKEAMVLTDK
                     EIIELARYGEILEEHYGLPQDIEWGVEKNKIYILQSRPITTISSEKKAAGSAASGSGK
                     ILLNGLGAAPGVASGIVKIITSGRDLDKIKQGDIMVTKMTMPDMVPGMKRAGAIVTDE
                     GGMACHAAIVSRELGCPAVVGTKKATSILKDGMEITVDGGKGIVYEGIVAQAAPAKAA
                     LPATAGGVFYSKPVTATEIKVNVSEPDRAEVAAATQADGVGLLRIEHMILGMGKTPNW
                     YIRSGKSEEYISELVNGIKVVADAFYPRPVWVRTLDAPTDEFRAMEGGEGEPHEHNPM
                     LGWRGIRRDMTETDHFRLEVKAFKRLHEMGLTNVGIMLPMVQHVSEFQKAKSIMRDEG
                     LDLDKIDVGIMIETPGAALTIEDFIEDGIDFISFGTNDLTQYTLAVDRNNENVAGLYS
                     ELHPAVLKLIEYVVEQCREAGVKTSICGQAGSYPEMAKKLVEMGITSISANIDAVATV
                     RDTV"
BASE COUNT          605 a          611 c          750 g          476 t
ORIGIN      
        1 cagctcctcg atatccttga ggggaagaca tcgctccaag agcatccttc tcgccgctat
       61 tgccccggaa aagcgtttga acatgcagca tagacttgaa tgatcctgga taaatgcttt
      121 tgtatatatt ctatatatca tgtgaaagct tcatataatg ttatgatgga ggggtgggca
      181 tattgacctg taatattggg cggatgattt aatggaagct gttgtatggt tggaggatgt
      241 cggcaaggaa gacctgtcca ttgtcggcgg caaaggtgca agcctcggcg agatgatcag
      301 tataggcgtt cccgttcctg gtggttttgc ggtcactgct caggccttca gggatttcat
      361 taaccgggcg ggcatcgcgg ataagctctt tgaggcactt aacgtcgatg tgaaccagga
      421 gactgagctt catcgagccg agcagacggc aaaaaagctc atcatggacg ccaaggtgcc
      481 gaaggatatt gaagaggcca taaaaacccg ctacgaggag ctgtgcaaaa gggaaggaaa
      541 gcaggttttc gtggcggtaa ggtcaagcgc caccgccgag gatctgcccg acgccagctt
      601 cgccggccag caggagacct acctcaatat gcgcggcgca gaggacgtat tcaatgccgt
      661 gcgcaagtgc tgggcatccc tttacggggc cagggctatc ttctaccggg ttgagcaagg
      721 cttcgagcac aataaggtca atctatccgc catagtgcag ttgatggtgg actcagagaa
      781 ggcaggagtg atgttcagct cccagccgag cactggcgag ccgctggtgg tcattgaggc
      841 cgcatggggt ttgggggagt cagtagtcag tggcagcgta tcaccggaca actatgtggt
      901 ggaccggaag accaagaaga tagtgaacaa gtatatcgcc accaaagaga tcatgatcac
      961 cagagatccc aagacccata agaccaccac taccaaggtc cctgccaaga agaaagaggc
     1021 aatggtcctc accgataagg agataatcga gctggccagg tacggcgaga tcctggaaga
     1081 gcattacggc ctccctcagg atatcgagtg gggtgtagag aagaacaaga tctacatcct
     1141 ccagtcccgt cccatcacca ccatcagcag cgagaagaag gccgccggct cagcagcatc
     1201 cggtagcggc aagatccttt tgaacggcct tggtgcagca ccaggtgtgg ccagcggcat
     1261 tgtgaagatt atcaccagcg gcagagacct ggataagatc aagcagggcg acatcatggt
     1321 caccaagatg accatgccgg atatggttcc tgggatgaag cgggccggtg ccatcgtcac
     1381 cgatgagggc gggatggcct gccatgcggc catcgtcagc cgagagctgg gctgtcctgc
     1441 agtggtggga accaagaagg ccaccagcat tttgaaggac ggcatggaga tcaccgtgga
     1501 tggaggcaag gggatagtct acgagggcat agtggctcag gctgcgccag cgaaggctgc
     1561 ccttccagcg actgcaggag gtgtcttcta ctccaagccc gtgacggcca cggagatcaa
     1621 ggtcaatgtc agcgagccgg acagggcaga ggtggcggct gctacccagg ccgatggggt
     1681 gggcctgctc aggatcgagc atatgatctt gggcatgggc aagacaccca actggtatat
     1741 caggagcggg aagtcagagg agtacatatc ggagctggtc aatggtatca aggtcgttgc
     1801 cgatgctttc tatccccggc cggtctgggt gcgcactctg gatgccccca cggacgagtt
     1861 ccgggccatg gaaggcggag aaggagagcc tcatgagcac aaccccatgc ttggctggag
     1921 gggcatccgg cgggatatga ccgagacaga tcacttccgc ctggaggtca aagccttcaa
     1981 gaggctgcat gagatgggcc tgaccaatgt gggcatcatg ctgcccatgg tccaacatgt
     2041 cagcgagttc cagaaagcca aatccataat gagggacgag ggcctggacc tggataagat
     2101 cgatgtaggt atcatgatcg agacaccagg cgccgctctg accattgagg acttcattga
     2161 ggatggaata gatttcatct cctttggcac caacgacctc acccagtata ccctggcagt
     2221 agatcgaaac aatgagaatg tggccgggct gtactccgag cttcatcccg ccgtcctgaa
     2281 gctgatcgag tatgtggtgg agcagtgccg cgaagcggga gtcaagacct ccatatgcgg
     2341 ccaggctggc tcctatccgg agatggccaa aaaattggtc gagatgggta tcaccagcat
     2401 atccgctaac atcgatgccg tggccaccgt cagggatacc gt
//
LOCUS       DAOSGP010000209         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_42345_length_2430_cov_15.961684, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000209 DAOSGP010000000
VERSION     DAOSGP010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42345_length_2430_cov_15.961684"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1192)
                     /gene="ilvC"
                     /locus_tag="PLV49_06040"
     CDS             complement(200..1192)
                     /gene="ilvC"
                     /locus_tag="PLV49_06040"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOG98440.1"
                     /translation="MKIYHDQDADLKALSDKTIAIVGWGNQGHAQGENLKDSGLNVIA
                     ADIPGSRAWKRAEADGVKAMSVADAARAADYIQILLPDEYQGKIYRDEIAPNLEEGNV
                     LGFSHGFNIRYFQIVPPENVDVVMVAPKGPGDLVRRTYQEGKGVPCLVAVEQDHSREA
                     LKKALAYAKGIGGTRAGVIETTFTEETETDLFGEQVDLCGGVTEMIKAAFETLVDAGY
                     QPEIAYFEACHELKLIVDLIYEGGFMRMWNNVSNTAEYGGMTRGHRIINEQSREEMLD
                     ILAEIQSGEFAREWILESQAGLPVKKSLEKMESEHPIEEVGAQLRAMMPWLEKK"
     gene            complement(1220..1711)
                     /gene="ilvN"
                     /locus_tag="PLV49_06045"
     CDS             complement(1220..1711)
                     /gene="ilvN"
                     /locus_tag="PLV49_06045"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOG98441.1"
                     /translation="MKHIIAVIVENKSGVLTRIAGLFSRRSFNIDSLSVGATDNPDYS
                     RMTISVQGDRDTLEQVIKQLSKLINVIRVSELDPVESLERELAIIKVSVDRESRSEVM
                     QIVNIFRAKIIDVSQRSMIIEVTGDEEKVEAIIQLLRQFGIKELARTGKVSMVRGTRV
                     IKV"
     gene            complement(1712..>2430)
                     /locus_tag="PLV49_06050"
     CDS             complement(1712..>2430)
                     /locus_tag="PLV49_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG98442.1"
                     /translation="WKKDHPMRYIPDMNLIKPQYVIEKLCELAPDAIITTEVGQNQMW
                     AAQFFVHKDPQRFISSGGLGTMGYGFPAAIGAKMGRPECTVIDVAGDGSFLMNSQELA
                     TAVVNDIPVKVAVLNNGCLGMVRQWQELFFEKRYSATILGRTSPDFVKLAEAYGAVGL
                     RATKPYEVEDVIRSALATDRPCVMDFIVNPAEKVSPMVPAGATLSEILELEWCDEAQC
                     GHLERIYAEERKGVLGEEGY"
BASE COUNT          470 a          802 c          588 g          570 t
ORIGIN      
        1 tacagcttcc gaaacgcggc caggtggccg cgcttctgaa actgctcctc cctcctgtga
       61 tgtagttctt ttggcttttt tgataatcgt ttatctcgag gccatagatc atggcactgc
      121 gtccgagcac gaggcactcg cgcccgtccg caacatgcta caacctgcca tgatataaag
      181 cttacttctg ctcttgcacc tatttcttct ccagccaggg catcatggcc cgcagctggg
      241 caccaacctc ttcgatgggg tgctcggact ccatcttctc cagggacttt ttcaccggaa
      301 ggccggcctg gctctccagg atccactccc gggcgaactc gccggactgg atctccgcca
      361 agatatccag catctcctca cgagactgct cattgattat cctgtggccc cgggtcatgc
      421 ccccgtactc agcggtgttg gatacattgt tccacatgcg catgaatccg ccctcgtaga
      481 tcagatccac aatcagcttc agctcgtgac acgcctcgaa ataggcgatc tcaggctggt
      541 atcctgcatc caccagggtc tcgaaggccg ccttgatcat ctccgtcacc ccgccgcaca
      601 ggtccacctg ctccccgaag agatcggtct ccgtctcctc agtgaaggtg gtctcgatga
      661 cccctgcccg tgtgccgccg atgcccttgg cataggccag ggcctttttc aatgcctctc
      721 ttgaatggtc ctgctctact gccaccaggc acggaacccc cttgccctcc tggtaggtcc
      781 tgcgcaccag gtcgccggga cccttgggcg ctaccatgac cacatcgacg ttctcaggcg
      841 ggacgatctg gaagtagcgg atgttaaagc cgtggctgaa tccaaggacg ttgccctcct
      901 caaggtttgg agcgatctcg tcccggtaga tcttgccctg atactcatcc ggcaggagga
      961 tctgaatgta gtccgccgct cgtgccgcat cggcaacgct catggccttc actccgtccg
     1021 cctccgccct cttccaggcc cgggatccgg gaatatcggc cgctatcaca ttgagtccgg
     1081 agtccttaag gttctccccc tgggcatgcc cctgatttcc ccatcccact atggcgatgg
     1141 tcttgtccga caatgccttc aagtctgcat cctgatcatg gtatattttc accaattttt
     1201 attcctccta aattaaaaat taaaccttga taacccttgt gcctcttacc atcgagacct
     1261 ttccggtgcg ggccagctcc tttatgccga actggcgcag aagctgaatt atggcctcga
     1321 ccttctcctc gtctcccgtg acctcgatga tcatcgacct ctgggagaca tcgatgatct
     1381 tggcccggaa gatgttcacg atctgcatga cctcgcttct gctctcccgg tccacgctca
     1441 ccttgatgat ggccagctcc ctctccaggg attcgaccgg gtccagctcg ctcacccgga
     1501 tgacattgat cagcttggac agctgcttta tcacctgctc cagagtgtcc ctgtccccct
     1561 gaacggagat ggtcatgcgg gaataatccg gattgtcggt agcccctaca gagaggctat
     1621 cgatattaaa agaccggcgg ctgaagaggc cagcgatgcg ggtgagcacc ccggacttgt
     1681 tctccacaat gacggcgatg atatgcttca tctagtagcc ctcctcgccc agaactcctt
     1741 tcctctcctc ggcataaatc ctctccaagt gaccgcactg agcctcgtcg caccactcca
     1801 gctccagtat ctcgctcaaa gtagctcccg ccgggaccat aggcgagacc ttctctgccg
     1861 gattgacgat gaaatccatc acgcagggcc gatctgttgc cagagcgctg cgaatcacat
     1921 cctcgacctc atagggcttt gtggccctca atcccaccgc tccataggcc tcggcaagct
     1981 tcacgaaatc cggactggta cggccaagaa tagtggccga gtacctcttc tcaaagaaca
     2041 gttcctgcca ctgccgcacc attcccagac agccgttgtt cagcactgcc actttgaccg
     2101 ggatgtcgtt gaccacagcc gtcgccagct cctggctgtt catcaggaag ctgccgtccc
     2161 cggcgacatc gatgaccgtg cactcaggcc ggcccatctt ggcgccgatg gcagctggaa
     2221 acccataccc catcgtcccc aggccgccgg aggatatgaa cctctgagga tccttatgca
     2281 caaagaattg cgctgcccac atctggttct gtccgacctc tgtggtgata atggcatcgg
     2341 gcgcaagctc gcagagcttc tcgatcacat actgaggctt gatcaggttc atgtccggaa
     2401 tatagcgcat gggatgatcc ttcttccagc
//
LOCUS       DAOSGP010000210         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_42538_length_2423_cov_19.376267, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000210 DAOSGP010000000
VERSION     DAOSGP010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42538_length_2423_cov_19.376267"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..>1167
                     /locus_tag="PLV49_06055"
     CDS             80..>1167
                     /locus_tag="PLV49_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOG98443.1"
                     /translation="MPDKKKDKKLTTNVGAPVADNQNVLTAGQRGPMLLQDVWYLEKL
                     AHFDREVIPERRMHAKGSGAYGTFTVTHDITRFTRAMIFSKIGKKTDLFARFSTVAGE
                     RGAADAERDIRGFAVKFYTEEGNWDLVGNNTPVFFLRDPLKFPDLNHAIKRDPRTGMR
                     SAKNNWDFWTSLPEALHQVTITMSDRGIPYSFRHMHGFGSHTFSMINAKNERVWVKFH
                     WVTQQGIKNLTDAEAEAIIAKDRESHQRDLFDSIEKGDYPSWKLFIQVMPEKEAQTVP
                     YHPFDLTKVWYHGDYPLIEVGVMELNRNPENFFAEVEQAAFNPANIVPGIGFSPDRML
                     QGRLFSYGDAQRYRLGVNHHLIPVNRAR"
     assembly_gap    1168..1267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1268..1411
                     /locus_tag="PLV49_06060"
     CDS             <1268..1411
                     /locus_tag="PLV49_06060"
                     /inference="COORDINATES: protein motif:HMM:NF018351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HOG98444.1"
                     /translation="FENTARAMGDAPKMIKIRHIGNCYKADPAYGEGVARALGISMSE
                     IHK"
     gene            1470..2045
                     /locus_tag="PLV49_06065"
     CDS             1470..2045
                     /locus_tag="PLV49_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOG98445.1"
                     /translation="MKSIKGTQTEKNLLLSFAGESQARNRYSYFGSQAKKEGYEQIAA
                     IFLDTADNEKEHAKVFFKLLEGGELEISGSFPAGVIGDTKANLMEAAEGEKFENTKMY
                     PEFAATADKEGFPEIAEKFRNVCKVEKGHEDRYRALLKNVEEGKVFKKDTKVAWRCRN
                     CGYIHEGTEAPDECPACAHEQAYFELMAKNY"
     gene            2152..>2423
                     /locus_tag="PLV49_06070"
     CDS             2152..>2423
                     /locus_tag="PLV49_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HOG98446.1"
                     /translation="MAELTGVPGKIELKFRSGKGVEEFEQKYALTNSETASFLRGLAG
                     EIEAGGEVSVKYGSINISINPEPPIKVEVECEKDELEIEIKLKAKK"
BASE COUNT          644 a          559 c          655 g          465 t
ORIGIN      
        1 atgaacttct tggctgtggg ccaatgcttc ctgctatctg ctcaggcgca aagggattga
       61 acctcaggag agaatagtta tgccggataa aaagaaggat aagaagctga ccacaaatgt
      121 cggtgcacca gtggccgaca accagaatgt attgacggca ggacaacgtg gaccaatgct
      181 tctccaggat gtatggtacc tggaaaagct cgcccatttc gaccgggagg taattcctga
      241 gagaaggatg catgccaagg gctcaggtgc ctatggaaca ttcactgtta cccatgacat
      301 aaccaggttt accagagcta tgatcttctc gaagatcggc aagaagaccg atctgtttgc
      361 gcgcttttcc acagtagccg gtgagagggg ggcagcagat gccgagagag acatccgcgg
      421 ctttgccgtc aagttctaca ccgaggaggg caactgggac ctggtgggaa acaacacccc
      481 tgtcttcttc ctgagagatc cgctcaagtt ccctgacctg aaccacgcca ttaagcgcga
      541 cccgaggacg ggcatgcgta gcgccaagaa caactgggac ttctggacct ctcttcctga
      601 ggctctgcac caggtgacaa tcaccatgag cgatcggggt atcccttact ccttccgcca
      661 catgcatgga tttggcagcc ataccttcag catgatcaat gccaagaacg agagggtctg
      721 ggtgaagttc cactgggtaa cccagcaggg aatcaagaac ctgaccgatg ccgaggcgga
      781 ggcgatcata gccaaggatc gggagagcca tcaacgcgat ctattcgata gcattgagaa
      841 gggagactat cctagctgga agctgttcat ccaggtaatg cccgagaagg aggcacagac
      901 cgttccctat caccccttcg acctgaccaa ggtctggtac catggagact atccgctcat
      961 tgaggtgggc gtaatggagc tcaaccgcaa tccagagaac ttctttgccg aggtagagca
     1021 ggcggcattc aatccggcca atatagttcc cggcataggc ttctctccgg acaggatgtt
     1081 gcagggcagg ttgttctcct atggcgatgc ccagcgctac cgcctgggtg tgaaccacca
     1141 tctgattcca gtcaaccggg cccgctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnttt gagaacaccg ctcgtgccat gggagatgct cccaaaatga tcaagatccg
     1321 ccatataggc aattgctata aggccgatcc agcctacggc gagggcgtgg ccagggctct
     1381 tggcatatcc atgagcgaga ttcataagta gatcgaattg aattgaaaac cagatcaaat
     1441 tgatatcaag ataattcgga gatgagagat tgaagagcat taaaggcaca cagacagaga
     1501 agaatttgtt gctctccttt gcaggagaat cgcaggccag gaacagatac tcctattttg
     1561 gctcccaggc caagaaggaa ggctacgagc agatagcggc aatattcctg gatacggcag
     1621 acaatgagaa ggagcacgcc aaggtcttct tcaagctgct ggaaggagga gagctagaga
     1681 tttccggctc attcccggct ggcgtgatcg gggataccaa agccaacctc atggaggcgg
     1741 ccgagggaga gaagttcgag aacaccaaga tgtatcccga gtttgccgcc acggcagaca
     1801 aagagggctt cccggagata gctgagaagt tcagaaatgt ctgcaaggtg gaaaagggcc
     1861 atgaggacag ataccgggct ctgctgaaga atgtagaaga gggcaaggtc ttcaagaaag
     1921 ataccaaagt ggcctggagg tgccgaaact gcggatacat ccatgaaggc accgaggctc
     1981 cagatgagtg cccggcatgc gctcatgagc aggcttactt cgagctgatg gccaagaact
     2041 attgagaaat cgagctggac aagctgaaat tcagatgaat gggcttgaaa catcgccctt
     2101 tcatcagcca aaccgattta tagatcgagc cctaaaagaa cagcgattgt tatggctgaa
     2161 ttaacaggtg ttccgggaaa gattgagctt aaattccgct caggcaaagg ggttgaggag
     2221 ttcgagcaga aatatgccct caccaactcc gagacggcct catttctaag aggtcttgct
     2281 ggagagatcg aggctggcgg agaggtctca gtgaaatatg gcagcatcaa tatttccatc
     2341 aatcccgagc ctccaatcaa ggtcgaggtg gagtgcgaga aggatgagct tgagattgag
     2401 ataaagctca aggcaaaaaa gga
//
LOCUS       DAOSGP010000211         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_42608_length_2420_cov_16.046512, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000211 DAOSGP010000000
VERSION     DAOSGP010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42608_length_2420_cov_16.046512"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLV49_06075"
     CDS             <1..632
                     /locus_tag="PLV49_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HOG98447.1"
                     /translation="EVLWSFRMKQSMSSTLDRAALVLGILFFFGVMIEDYRNLLGQVM
                     DHLLGWLPQTGLSFPVILFILAAITGLYASLVQKYTMDWDFMRRQQEKMRALQTNMKA
                     AQLAGDQSKVQSLQTEQMKMVSEQGKMMQMQFKPMLYIGIISIPLFMWIYFYIHNSGG
                     ITMTFPFWGTHGITEMVLGPILYWFYWYFVCSLPVSQIIRKALDIGSMS"
     gene            643..1167
                     /locus_tag="PLV49_06080"
     CDS             643..1167
                     /locus_tag="PLV49_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG98448.1"
                     /translation="MIITVSGSPGTGTTTLARALSIQLGLPWVNSGELFRKIASEKNI
                     SVKEMNRMAEKGPEIDYLIDDAQRSLAREGQGVFEGRLSGHLLDADLKVLLKSDLRTR
                     AERISKRESKLLEDACQETRLREESEARRYKKYYNIDINDFNVYDLVVDTGRFNESGT
                     LAIVLAAARSRQCP"
     assembly_gap    1176..1275
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1276..2292
                     /locus_tag="PLV49_06085"
     CDS             <1276..2292
                     /locus_tag="PLV49_06085"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HOG98449.1"
                     /translation="PADKKRSVLVKRNGKTDPAYGTSPQERSLEMHLRLGAINLDKTS
                     GPTSHEVVAWVKRILEVEKAGHSGTLDPKVTGILPVLLGDATRVMDTLLLAGKEYICL
                     MHVHKPVPKKQIIDVCGQFVGPILQKPPLKSSVVKELRTRTIYYLEVLEIDGQHVLMR
                     VGCEAGTYIRKLCYDIGLALGTGANMEELRRTRAGPFREDETLVTLHQLMDAHVKYME
                     TGNENLLRRVILPVEAALVHLPRLVIADNAVDAICHGAPLAAPGLLSLQTDINRGDGV
                     VLFTLKGEAVAVAQAELSSEEMLASKSGIVAATERVIMEPGTYPKAWKLAERDGSTQP
                     ETKR"
     gene            2313..2412
                     /locus_tag="PLV49_06090"
     tRNA            join(2313..2348,2365..2412)
                     /locus_tag="PLV49_06090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2345..2347,aa:Ser,seq:gga)
BASE COUNT          534 a          582 c          703 g          501 t
ORIGIN      
        1 cagaagttct ttggagcttc cgcatgaagc aatccatgtc cagtacgctg gacagagcgg
       61 ccctggtact gggcatcctc ttttttttcg gggtgatgat cgaggattac aggaacctct
      121 tagggcaggt catggatcac cttctgggat ggctgcccca gacggggctg tcctttccgg
      181 tcatcctctt catcctggcg gccattaccg gcctttatgc cagcctggtt cagaagtaca
      241 ctatggactg ggattttatg cgtcgccagc aggagaagat gagagccctg cagaccaaca
      301 tgaaggcagc tcagctggct ggggaccagt ccaaggttca gtctctgcag actgagcaga
      361 tgaagatggt ctcagagcag gggaagatga tgcagatgca gttcaagccc atgctctaca
      421 tcggaataat atccattccc ctgttcatgt ggatatattt ctacattcat aactccggtg
      481 gaatcaccat gacctttccc ttctggggga cgcatggtat caccgaaatg gttctggggc
      541 cgattctcta ctggttctac tggtatttcg tatgctcatt gcccgtatcg cagatcatca
      601 gaaaggctct ggacataggg agcatgagct gaagatgata atatgataat caccgttagc
      661 ggctcgccgg gcacagggac caccaccctg gcccgggctc tttctatcca gcttggcctc
      721 ccctgggtca actccgggga attgttccgc aagatcgcca gcgagaagaa catatccgtc
      781 aaggagatga accgcatggc agagaaggga ccagagatcg attatctgat cgatgatgcc
      841 cagaggtctc tggccagaga gggacaggga gtcttcgagg gcaggctttc cgggcacctt
      901 ctggatgccg atctcaaagt tctcttgaag tccgatctga ggacaagagc ggagcgcata
      961 tccaagcggg agtccaagct gctggaggat gcctgtcagg agacgaggct gcgggaggag
     1021 agcgaggccc gcaggtacaa gaaatactat aacatcgaca tcaatgactt caacgtatac
     1081 gatctggtgg tggataccgg caggttcaat gaatcgggaa ctttggccat agtgctggcg
     1141 gcggcgaggt caaggcaatg tccgtgattc acaggnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnncctgc ggacaagaag aggtcggtcc tggtcaaaag gaatggtaag
     1321 acagatcccg cttatggcac ttccccgcag gagcggagcc tggagatgca ccttcgttta
     1381 ggggcgatca atctggacaa gacctccggc ccgacgagtc atgaggttgt ggcctgggtg
     1441 aagaggattc ttgaggtgga gaaggctgga catagcggca ctctcgatcc caaggtgact
     1501 ggcatcctgc ccgttctcct gggggatgca acaagagtta tggataccct tcttctggcg
     1561 ggaaaggagt acatctgcct gatgcatgtt cataagccag tgcccaaaaa gcagatcatc
     1621 gatgtctgtg gtcagtttgt cggacccata ctgcaaaaac ctcccctgaa gtcgtccgtg
     1681 gtcaaggaat tgcgcaccag gaccatctat tatctggagg tgctggagat cgatgggcag
     1741 catgtgctga tgagggtggg ctgcgaagct ggaacctaca tccgaaagct atgctacgat
     1801 atcggccttg ccctgggcac gggggcgaac atggaggagc tgcgccggac ccgtgccggt
     1861 ccttttcgcg aggatgagac cctggtcacc ctgcaccagc tgatggatgc ccatgtaaaa
     1921 tatatggaga cggggaatga gaacctgctt cgcagggtga ttttgccggt ggaggcagct
     1981 ttagtgcatc tgccccgact ggtgatagcg gacaatgcgg tggatgccat atgtcatggc
     2041 gcgccccttg ctgcccccgg cctattaagt ctgcagacgg acatcaatcg gggcgatggt
     2101 gtggtgctct tcacgctgaa aggagaagca gtggccgttg cccaggcgga gctctcgagc
     2161 gaggagatgc ttgcctcaaa gtctggaatc gtggctgcga ccgaaagggt aataatggag
     2221 ccgggcacct atccgaaggc gtggaagctg gccgaaaggg acggctccac ccagccggaa
     2281 acgaagcgat aaaaccaaaa atccaaacct gtgccgaggt cgtctagcgg tagggcgcca
     2341 gcctggagtg agtttctcct ggatagctgg tgactcgcaa gggtctcgtg agttcgaatc
     2401 tcaccctcgg cgctatttta
//
LOCUS       DAOSGP010000212         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_42713_length_2416_cov_18.546379, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000212 DAOSGP010000000
VERSION     DAOSGP010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42713_length_2416_cov_18.546379"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLV49_06095"
     CDS             complement(<1..282)
                     /locus_tag="PLV49_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG98450.1"
                     /translation="MDIVSRKYGIASLIALILIALGFNAAGAEYSVGSESDDWWTAYP
                     DQSTGAGVDVSHPDWVLDALENKPVLIYVHKECDYCVPQTEAVQKIIDEF"
     gene            806..1225
                     /locus_tag="PLV49_06100"
     CDS             806..1225
                     /locus_tag="PLV49_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG98451.1"
                     /translation="MSCISKEAVDRMTRLIGDRDKAECKVNRLRSLTIDLDEEELKTE
                     SEIFQAMADPCRLKILSLLREGELCACEIMIGVDRPQSSTSHHLSILKNAGLIKERKD
                     GRWSRYRLSEGAVIEMLNLSQIMKESKDERGNNNDKV"
     gene            1212..2210
                     /locus_tag="PLV49_06105"
     CDS             1212..2210
                     /locus_tag="PLV49_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG98452.1"
                     /translation="MTKFNFAIPAILMISLLTLAPLANAGCCSEGTWDPSGFLNSDAG
                     TGLPSQPIQSAQAEGSQESAASSSSSTQNPPDRLASYPNGSIMKPMKSVSSSDVIIDA
                     SNGDGYAASHIKNAIHIPSKDLLDGNGNIKGDEELSRLLGEAGLSKEDSVVVYGSAES
                     SGEAQLAFLVLRHLGQEDVRLLDGSLDDWTDAGLPVEAQETIIHAAEYIPSARPGVIA
                     EYDYVKSGQARIVDVRPFVDFGKGRIPGSTALDPANVIKGDKIKNGGDLGTVFSRLDK
                     DMPIVVYSDDYSQSALVAYSLQLMGYEAAVYSWGDWQSHEGKGDDSDSKYVKLGRT"
BASE COUNT          640 a          581 c          601 g          594 t
ORIGIN      
        1 gaattcgtcg attatcttct gcacggcctc ggtctgtggt acacaataat cacattcttt
       61 atgcacatag atcagcacag gcttgttctc cagagcgtcc aatacccaat caggatggct
      121 gacatcgaca cctgcacccg tggactgatc gggatatgct gtccaccagt catcgctttc
      181 cgatcctact gagtattcag ctcccgcggc attgaagcct aaagcgataa gaattaatgc
      241 aattaatgag gcaatgccat attttctcga tacaatatcc atggtacact ctccttttcc
      301 ccactcattc ccatgccgat ccaactatca catcgacatc ttgctgttgg tatgaggagc
      361 taatctcagc acatcgacat tattggatat aagactttcg tcaattcgat ctatatagac
      421 atacctgagg aaaacatttg ctttaagatc atgcatggta ttatatttaa aatgaattat
      481 ggatcaatgg ttgttaaata agatttgcag acagcaggaa tacatatatc aatttcacta
      541 tatataatta tcgtcagatg tcgtacctgc tcttcatcgc ctgactggaa actcttatat
      601 atcgagattg ctggatatgg tgttgtattg cttatttgag gtcactatgg aatgcgaaat
      661 atgcaataga agcgggaaat cctttttcct ggttacacat aaagaaaagg gaagaatcag
      721 gatctgtggc gattgcctgc agaaagaaag ggacaatctc cttgctcaaa aaagctgcac
      781 atgctgctga ggtgatagga gactcatgtc atgcatatcc aaagaggccg tcgacaggat
      841 gaccaggctc atcggcgatc gcgataaggc agagtgcaag gtgaacaggt tgcgctccct
      901 gaccatagat ctggatgaag aggaactcaa gaccgaatct gagatctttc aggccatggc
      961 cgatccctgc cgcctcaaga tactctctct tctgcgggag ggggagctat gcgcctgcga
     1021 gatcatgatc ggcgtggacc gaccccaatc cagcacttct catcatctct ccatcctcaa
     1081 gaatgccggt ctgatcaaag agcgcaaaga cggaagatgg tccagataca ggctctcaga
     1141 aggggcagtg attgagatgc ttaatctgtc acaaattatg aaagaatcca aagacgaaag
     1201 aggtaacaac aatgacaaag tttaattttg ctattcctgc aattctcatg atttctttgt
     1261 taactctagc tcccctggcc aatgccggct gctgcagtga aggcacctgg gaccccagcg
     1321 gatttctgaa ctccgatgct ggcacaggcc tgcccagtca gcccatccag tccgcccagg
     1381 cagaaggatc gcaagaaagc gctgcatcaa gctccagcag cactcaaaat ccgcccgata
     1441 ggctggcatc ataccccaat ggcagtatca tgaagcccat gaagagcgtc tccagctctg
     1501 atgtgataat agatgcatcc aacggagatg gctatgctgc atctcatatc aagaacgcca
     1561 ttcacatccc cagcaaggac ttgctcgacg gcaatggcaa tatcaagggc gatgaagagc
     1621 tttccaggct cctgggtgag gcaggcctct ctaaagagga ctccgtggtt gtatatggaa
     1681 gtgcagaaag ctccggcgag gcgcagttag cattcctggt cctgagacat ctggggcagg
     1741 aggatgtgag gctattggac ggaagcttgg atgactggac ggatgcaggc ctgccggttg
     1801 aggcccagga gactataatt catgctgctg aatatattcc ctccgcaagg cctggcgtga
     1861 tagcagaata cgattatgtt aagtccggcc aggctcggat cgtggacgtc cggccttttg
     1921 tggactttgg aaaaggccgc atacccggct ccacagccct ggacccggcc aatgtgataa
     1981 aaggagataa aatcaagaac ggtggcgatc taggaaccgt cttcagccgg ctggataagg
     2041 atatgcctat tgtggtctat tccgatgact acagccaatc tgctctggtg gcatattctc
     2101 tgcagctcat gggctatgag gctgccgtct actcctgggg agactggcag tcgcatgagg
     2161 gcaagggaga tgattctgat tctaagtacg taaagcttgg caggacataa ttgcattttt
     2221 aagggggatg gtggagcgat atgtcaagtg gatcaagtgg gtcaaatggg gtgagcaagg
     2281 ggctggattt tctctctcgc taccttacca tctggatatt cgtggccatg atcctgggaa
     2341 tcgcccttgg ctattatgct cccgggataa cccgtttcat cctcagcctg cagatcggca
     2401 cgacctcaat tcccat
//
LOCUS       DAOSGP010000213         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_42832_length_2412_cov_16.729317, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000213 DAOSGP010000000
VERSION     DAOSGP010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42832_length_2412_cov_16.729317"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLV49_06110"
     CDS             complement(<1..607)
                     /locus_tag="PLV49_06110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696753.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOG98453.1"
                     /translation="MTKEYKTITEISGPLIFVEKTEPVGYMELVEIRTGGDLKRGQVL
                     DSSDDIVVIQIFEGTGGLSKDSAVSFTGDVIKMPLSPAIVGRVLSGSGKPRDGGPEIV
                     PDVLRDIAGAAINPASREKPRAFIQTGISTIDGTNTLVRGQKLPIFSGAGLPHNDLAL
                     QIARQAKALGEAESFAVIFCAMGITNEEAQHFLADFERTGAL"
     gene            complement(620..2350)
                     /locus_tag="PLV49_06115"
     CDS             complement(620..2350)
                     /locus_tag="PLV49_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOG98454.1"
                     /translation="MEVGKIKRVAGPLVQATGLKASMYDLVLVGEEGLMSEVIGISGN
                     KHIIQVYEDTGGVRPGEPVKETGAPLVAQLGPGILTQIYDGIQRPLPKLAEASGDFIS
                     RGLFVDGIDHKKKWEFKSVVKKGDTVKPGQAIGEVQEQLLIKHKIMVPPNLKGGAIKE
                     IYDGNFTVDETIAVLDDGTKLSMMHKWPVRQARPVTEKLAPTIPLRTGQRVIDGFFSL
                     AKGGTAAIPGGFGTGKTVMQQTLSKWADVDIVVYVGCGERGNEMADLLHEFPELQDPR
                     TGRPLMERSIVYANTSNMPVAAREASVYTGMTTSEYYRDMGYDVLMTADSTSRWAEAM
                     REMGSRLEEMPGEEGYPAYLGARLADFYERAGRAEVLNGGQGSVSIVGAVSPAGGDFT
                     EPVTQNTLRMVKVFWALDSKLTQRRHFPSINWLDSYSLYDLDLEPWFVENVSADWNKN
                     KRRAMAILQENAELEEIVMLVGSDALPEDQQVTLEVARMIINFWLAQSAFHPVDTFSP
                     YKKQFDLLEAILKYRDYAFEAVQKGIPVGQITSVPSKDALAKVRLEAEYEPILAKVLT
                     QMDAEFKALK"
BASE COUNT          490 a          696 c          628 g          598 t
ORIGIN      
        1 ccagagcgcc ggttctctcg aagtcagcca ggaagtgctg agcctcttcg ttggtgatgc
       61 ccatggcgca gaaaataact gcgaaggact ccgcttctcc aagggccttg gcttgcctgg
      121 cgatctgcag agcaagatcg ttgtgaggca gaccagcgcc cgagaagatg ggtagcttct
      181 ggccgcgcac cagggtattt gtgccatcga tagttgagat gcccgtctgg ataaatgcac
      241 ggggcttctc acgggaagct ggattgatgg cagctcctgc aatgtctctc aatacatcgg
      301 gtacgatctc agggccgccg tctctgggct ttccagatcc ggacagaacg cgtcccacga
      361 tggcgggaga caatggcatc ttgatgacgt ctccggtgaa actgacggca gaatccttgg
      421 acagaccacc ggttccctcg aatatctgaa taaccacgat gtcatcagag gaatctagca
      481 cctgtcctct tttcagatct ccgccagttc tgatctcgac gagctccatg tagcccactg
      541 gctcggtctt ctccacgaag atgagtggtc cggagatctc agtaatcgtc ttgtattcct
      601 ttgtcatttt gaaccctcct cacttcagcg ccttaaactc ggcatccatc tgagtcagaa
      661 cctttgccag tatgggctca tactcagctt ccagtcttac cttggccaga gcatccttgg
      721 atggaacaga tgtgatctgc ccaacaggta tgcctttttg gactgcctcg aaggcgtagt
      781 ccctgtactt caggatggct tccaacaggt cgaactgctt cttgtaggga gagaaggtat
      841 caaccgggtg gaatgcgctc tgtgccagcc agaagttgat gatcatcctg gctacttcca
      901 gtgtgacctg ctgatcctca ggcagcgcat cggatcctac gagcatcaca atctcttcca
      961 gctcggcgtt ctcctgcagt atggccatgg cccgtctctt gttcttgttc cagtcagcgc
     1021 tgacgttctc tacgaaccag ggctcgaggt ccaggtcgta tagcgagtaa gaatccagcc
     1081 agttgatgga cgggaagtgc ctgcgttgtg tcagcttgga atcaagagcc cagaatacct
     1141 tgaccattct cagtgtgttc tgagtaacgg gctcggtgaa gtctccgccg gccggggaca
     1201 cggcgcctac aatgctaacg gaaccctgtc cgccatttaa aacctcggca cgtccggcac
     1261 gctcatagaa gtcggcgagt ctggcaccca ggtaagcggg atatccttcc tcaccaggca
     1321 tctcttccag acgagagccc atctctctca tggcctctgc ccaccgggag gtggagtcag
     1381 cggtcatcag cacatcgtag cccatatctc tgtaatactc tgaggtggtc attccggtgt
     1441 aaacagaagc ctcacgagca gccacaggca tgttggaggt attggcgtaa acgatggacc
     1501 tctccatcag aggcctgccg gttctgggat cctgcagctc ggggaactca tggagcaggt
     1561 cggccatctc attgccacgt tcgccgcagc ccacatagac cacaatgtcg acatctgccc
     1621 acttggacag ggtctgctgc ataaccgtct ttccggtgcc gaatccgccg ggaatggctg
     1681 ccgttccgcc tttggccagg gagaagaatc cgtctataac cctctgtcct gttctcagag
     1741 gaatggtggg tgccagtttt tctgtaactg gtcgggcctg tcttaccggc cacttatgca
     1801 tcatggagag cttggttccg tcatccaaga ccgcaatggt ctcatcaacg gtgaagtttc
     1861 catcatagat ctctttgatg gcaccgccct ttaggttggg tgggaccatg atcttatgct
     1921 tgataagaag ttgctcttgg acctcgccaa tggcctggcc gggcttgaca gtatcgccct
     1981 ttttcactac ggacttgaac tcccacttct tcttgtggtc gatgccgtct acgaacagac
     2041 cccggctgat gaaatctccg gatgcctctg ccagcttggg tagcggcctt tgaataccgt
     2101 cgtaaatctg ggtcagaatg cctggtccca gttgtgctac gagaggagcg cctgtctcct
     2161 tgacgggctc gccaggcctg acgccgcccg tatcctcgta tacctgaatg atatgtttat
     2221 tgccggaaat gccgatgacc tcgctcatca ggccttcctc gccgaccaat accaggtcgt
     2281 acatggaagc cttcaggcca gtggcctgaa ccagtggtcc ggcgactcgc ttaattttcc
     2341 ctacttccat aaatcaacac ctattgccct tttaatcttc tctctcatct ctgtgctctg
     2401 ctcggttccg at
//
LOCUS       DAOSGP010000214         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_42878_length_2410_cov_16.297240, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000214 DAOSGP010000000
VERSION     DAOSGP010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42878_length_2410_cov_16.297240"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..166)
                     /locus_tag="PLV49_06120"
     CDS             complement(35..166)
                     /locus_tag="PLV49_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98455.1"
                     /translation="MKSSRKAQAFIEALTKSSGMMIMPPEWIDILQIKANRHDEGKI"
     gene            268..1458
                     /locus_tag="PLV49_06125"
     CDS             268..1458
                     /locus_tag="PLV49_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG98456.1"
                     /translation="MILAAGEGRRCRPLTQTRSKVMLPVGNRPFMEYVIRALAANGIE
                     DIYVVVGYQKERVMDYFEDGIDFDVAITYIEQNELLGTAHALLKAEQYVDEEFLVVNG
                     DNLIDKRAVKELVSAKGENVILAALRRHTGDYGVLIVDNGLVTEIIEKPGRPCSGILN
                     TGSYKFSPDVFDALKQAPISERGSYEIPETIMQMIKEGSQIVPLITKGIWADAIFAWD
                     LLNANSLALGMDEPKIMGDVEEGAMIKGPVHLGEGSIIRSGSYLVGPVSIGEDCDIGP
                     NAVILPSTSVGDSARIGPHSEVRNSILMNDARIGSGCIISDSVIGASCTLKDQVVVET
                     GSCVVEIEGIFQRAEFGAILADDVSVGSRVLASPGTVVGTKASISSGAAIRGTINRDS
                     RVIC"
     gene            1458..>2410
                     /locus_tag="PLV49_06130"
     CDS             1458..>2410
                     /locus_tag="PLV49_06130"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HOG98457.1"
                     /translation="MCGIVAYIGDDKAGPILFDTLKRLEYRGYDSAGVAVISQGSVEV
                     LKSSGRIADLEKTYKSVGSPGEGMGIGHTRWATHGRPSDINAHPHTSGEIAIVHNGII
                     ENYLDLRELLTEMGYEFQSETDSEVLAHLIHFYYKGDLCAATLKALEKVEGSYAIAVM
                     AASSPYIVCARYESPLVIGKGSSSVFVASDVPALLPYTKDIIRLKDGNIARIYADRIE
                     VMDCSGAPLESEVERITWDTDAAEKGGYPHFMLKEIHEQPRAIRETIAGRISEIDGDV
                     RLSLGISEEEIMSLERVTIIACGTSYHAGMLARNLFVRAAGL"
BASE COUNT          570 a          577 c          713 g          550 t
ORIGIN      
        1 ctgatccagt cggtggtggg tattaccagt atattcatat tttgccttca tcatgccggt
       61 ttgcctttat ctgcaggata tcgatccatt caggcggcat tatcatcatg ccggacgact
      121 ttgtcagggc ttctataaaa gcctgggcct tcctactgct tttcattatg atctattcat
      181 attttttgag cacaattgac cgaaaagatc tagagcaaga tatgagcgta tccatttagc
      241 attctggctg tgatagattt gaagggagtg atattggcgg caggagaagg ccgcagatgc
      301 agaccgctta cccagacccg atccaaggtc atgcttcccg tgggcaaccg gccttttatg
      361 gagtacgtca ttcgcgctct ggctgcaaac ggaatcgagg acatctatgt ggtggtcgga
      421 taccagaaag agagggtgat ggactacttt gaggatggca tagactttga tgttgccatc
      481 acctacatcg agcagaatga gctcttggga actgctcatg ccctgctcaa ggccgagcag
      541 tatgtggacg aggagttcct ggtggtaaat ggagacaatc tcatagataa aagggctgtg
      601 aaagagctcg tctcggcgaa gggagagaat gtgatcctgg ctgccctccg ccgccatacc
      661 ggcgactatg gagtcctgat cgttgacaac ggattggtaa cggagataat agagaaacca
      721 gggcggcctt gctcggggat tttgaataca ggctcctata aattcagccc cgacgtcttt
      781 gacgccctca agcaggcccc catatcggag cggggctcct atgagattcc tgagaccatc
      841 atgcagatga tcaaagaagg atctcagatc gttcccctca taaccaaagg aatttgggcg
      901 gacgccatat ttgcctggga tctgctcaat gccaacagcc tggctttagg aatggatgag
      961 ccaaagatca tgggcgatgt ggaggaagga gcgatgatca agggtccagt ccatttgggc
     1021 gagggaagca ttattcgttc gggatcatat ctggtcggtc cggtctccat aggagaggac
     1081 tgcgatattg gccctaatgc cgtcatcctc ccctccacct ctgtcggcga ttcggcccgg
     1141 atcggtccgc attccgaggt cagaaacagc atattgatga atgacgccag gatcggatcg
     1201 ggatgcataa tctccgactc ggtaatcggg gcaagctgca ctcttaaaga tcaggtggtg
     1261 gttgagacag gttcctgcgt ggtggagatt gaagggatct tccagagggc ggagttcggc
     1321 gccatccttg ccgacgatgt gtccgtcgga tccagggtcc tggcgagccc gggtacggtg
     1381 gtgggcacaa aagcgagcat aagctctggg gcagcaatcc gcggcacgat taatagggat
     1441 agcagggtga tctgttaatg tgcggcattg tggcgtatat cggggatgat aaggctggac
     1501 ccatattatt tgataccctg aagaggctgg agtacagagg ttacgactct gcgggagtgg
     1561 ctgttatatc tcaggggtca gtcgaggtgc tgaagtcatc cgggcggata gcggacctgg
     1621 agaagaccta taagagcgtg ggaagcccgg gagagggcat gggcataggc catacccgct
     1681 gggctactca cggccggccc agcgacataa acgcccatcc ccacacatcc ggggagatcg
     1741 ctatagtcca taacgggatc attgagaact acctggatct gagagagctc ctcaccgaga
     1801 tgggatatga gttccagtcc gagacggata gcgaggtgct ggctcatctc atccactttt
     1861 actataaggg ggatctatgt gcagccaccc taaaagccct ggagaaggtg gagggctcct
     1921 atgccatagc agtgatggcc gccagctcgc cttacatcgt ctgcgcgaga tatgagagtc
     1981 ccctggttat aggcaaaggc agctcttccg tttttgtggc atccgatgta cccgcccttc
     2041 ttccttatac aaaagacatc atccgcctga aggacggcaa tattgcccgt atctatgcgg
     2101 acaggataga ggttatggat tgcagcggcg cgcctttaga gtcggaggtc gagcgcataa
     2161 cctgggatac ggatgcggct gagaagggcg gttatcctca tttcatgctc aaggagattc
     2221 atgagcagcc cagagccatc agggagacca tcgccggcag gatatcagaa atagatgggg
     2281 atgtgaggct ttccttgggc ataagcgagg aggagataat gtcgctggag agggtgacca
     2341 tcatcgcatg cggcacctca taccacgccg gtatgctggc cagaaacctc ttcgttcgcg
     2401 ctgccggcct
//
LOCUS       DAOSGP010000215         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_42903_length_2409_cov_19.423534, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000215 DAOSGP010000000
VERSION     DAOSGP010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42903_length_2409_cov_19.423534"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLV49_06135"
     CDS             complement(<1..823)
                     /locus_tag="PLV49_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOG98458.1"
                     /translation="MIAESMLIYIIVLLLTGMVVGFACGMLGVGGGFIMVPVQIWALT
                     SMGVDPTLSTRVAFGTSLAVILPTSLCGCHGHNCQGVVLWRPGVTLGLSGLVGAFLGG
                     SIAAHAPGELLRMIFGIIIMGGALRMIYARDLRRGRPEVVSSLLPYILWGVVVGVISG
                     LAGIGGGVILVPAMVIAMGFTMYQAVGTSTVAIAFNAVGGTIAYAINGWGVAGLPAYC
                     VGYIDLLQFVLLAGASMLTAHWGVNVTHKMSEEKLKRIFIALMILIGLKMMGLFDW"
     gene            complement(901..2100)
                     /locus_tag="PLV49_06140"
     CDS             complement(901..2100)
                     /locus_tag="PLV49_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOG98459.1"
                     /translation="MTLVREIKPGVFSIGAIDWDRRLFDALIALPDGTSYNSYLVRGK
                     NKIALIDAVDSTMADVLIENLNALGIKKIDYIIVQHAEQDHTGSLGRLVELYPDSAVA
                     GSARCLELLVEFGLVDKKRTQELKDGDVIDLGGKSLQIISAPWVHWPDTILSYLAADR
                     ILFSCDFLGSHLATSTLYADEAVVYRAAKRYYAEIMMPFRQHIRKHLERISNYDIDII
                     APSHGPIHARPEFILNAYGEWASDEVKNQVVLPFVSMHGSTKAMTDYIADALIARGIE
                     VKRFDLTNSDTGLLAEALVDASTIVIGTPTMLAGAHPLALYAAILANALRPKTRYASI
                     IGSYGWGGKMLEQITQAMPNLKLQLFDPVVAKGYPKEADFRRLDRLADEILAKHRELM
                     HAQSATN"
     gene            complement(2123..2287)
                     /locus_tag="PLV49_06145"
     CDS             complement(2123..2287)
                     /locus_tag="PLV49_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOG98460.1"
                     /translation="MANKYKCTLCGYIYDPKIGDPDSGIAPKTPFEELPDSWTCPQCG
                     ASKSDFEELK"
BASE COUNT          565 a          678 c          588 g          578 t
ORIGIN      
        1 tccaatcaaa taatcccatc atcttcaggc cgatgagaat catcagggcg atgaagatcc
       61 tcttgagctt ctcctctgac attttatggg tgacattcac tccccaatgg gcagtcagca
      121 tgctcgctcc tgctaagaga acgaactgaa gcagatcgat atagcccaca cagtaggcgg
      181 gaagcccagc taccccccat ccattgatgg cataggcaat cgtccctccc acagcgttaa
      241 aggcaatggc tacagttgat gtgccaaccg cctgatacat ggtaaagccc atggcaatta
      301 ccatagcagg aacaaggatc acgccgcccc ctattcccgc gagcccggag ataaccccaa
      361 ccactacccc ccatagaata taaggcaaaa ggctgctcac cacctcgggc cttcctcgcc
      421 gcagatcccg ggcatatatc attcgcagag ctcctcccat gattataatc ccaaagatca
      481 tcctgagcag ctcccccgga gcatgagcgg caatgcttcc gcccagaaag gcacctacca
      541 ggccagagag gcccaatgtc actcctggac gccagaggac tactccttgg cagttatgac
      601 catggcatcc gcagagcgac gtgggcagta tgaccgcaag ggaggtgcca aaagccaccc
      661 tggtggatag agttggatcc actcccatgg atgtcagagc ccagatctgc accggcacca
      721 tgatgaatcc acctcccacc cccagcatgc cgcaggcaaa tcccacaacc attcccgtca
      781 gcaggaggac gataatgtag atgagcatcg attctgctat catggccacc tgcaaacgaa
      841 tctcttaacg gtcatcttac cctgaggatc gatgagattg tattcatcat ttctgagctt
      901 tcaattggtc gcgctttggg catgcattag ttcacggtgc ttggctaaaa tctcatccgc
      961 cagccggtcc agacggcgga aatcggcctc cttgggatat cctttggcca ccactggatc
     1021 gaagagctgc agcttgagat tgggcatagc ctgggtgatc tgttccagca tcttccctcc
     1081 ccagccatat gatccgatta tggatgcgta cctggtcttc ggccggaggg cattggccag
     1141 gatggcagca taaagggcca ggggatgagc gcctgcgagc atcgtgggcg tgccaattac
     1201 aatggttgaa gcatcgacta gggcctcggc cagcaaacct gtatcgctgt tggtgaggtc
     1261 gaatcgtttg acctcgattc ccctggcgat cagagcatct gcgatatagt cggtcatggc
     1321 ttttgtcgaa ccgtgcatgg aaacaaaggg caaaaccacc tggtttttca cctcgtccga
     1381 ggcccattct ccataagcat ttagtatgaa ttctggacgg gcatggatgg ggccgtggct
     1441 gggggcaatt atgtcaatat cataattcga tatcctttcc aggtgctttc taatgtgctg
     1501 gcggaagggc atcatgatct ctgcataata tctctttgca gctctataga ccactgcctc
     1561 atctgcatat agagtgctgg tggctagatg cgatcccaga aaatcgcagc tgaagagaat
     1621 tctgtccgcg gcaagatagg acaatatggt atctggccag tgaacccagg gcgcggagat
     1681 tatctgcagg ctcttgcctc ccagatcgat tacatcgccg tctttcagct cctgcgttct
     1741 ctttttatcc accaggccga actcaaccag cagctccagg catctggccg agccggcaac
     1801 tgcggaatcg ggatatagct caaccagccg gccaaggctc ccggtgtgat cctgttcggc
     1861 atgctgtact atgatgtagt cgatcttctt tatgcccaga gcattcaggt tttctatgag
     1921 cacatcggcc atagtagaat ccacggcatc gatgagcgcg atcttgttct ttccccggac
     1981 cagatatgag ttgtagctgg ttccatcggg cagggcgatc agggcatcaa agagcctgcg
     2041 gtcccagtct atcgccccga tggaaaatac tcccggtttg atctctctta caagggtcat
     2101 ctctcctcca cctggctgct attcatttaa gctcttcgaa gtcgctcttt gatgcaccac
     2161 attggggaca ggtccagctg tctggcaatt cctcaaaagg agttttgggg gcgatgccgg
     2221 agtccggatc tcctatcttg gggtcataga tgtagccgca gagagtgcac ttatatttat
     2281 ttgccatgaa aaccactatc ctctttctgc taatcctctt tttagccgat tcaaggcatc
     2341 cgcattcctt tgacggaatc cggaagcctg aatctcaatt atctgaatct cactattctt
     2401 tctatttga
//
LOCUS       DAOSGP010000216         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_43635_length_2383_cov_13.970790, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000216 DAOSGP010000000
VERSION     DAOSGP010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43635_length_2383_cov_13.970790"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLV49_06150"
     CDS             <1..258
                     /locus_tag="PLV49_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG98461.1"
                     /translation="ETGLVIHPEKCFGCGNCVIACPVNVAAEPTRVGAGLGPEGDRVI
                     LRVEDGVVVANNLQECRRFGPNRVLCNACTATCPSKAIEFV"
     gene            269..1507
                     /locus_tag="PLV49_06155"
     CDS             269..1507
                     /locus_tag="PLV49_06155"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HOG98462.1"
                     /translation="MTEFNYKTCTGCSLICEDILFPDAGSGSVSQAKNLCRKGQAHYQ
                     ALFSERTGPLVEGSEVSLDQAIERAAEILRNASAPVIYGWSNCTLEAQGVGLALAAKI
                     GAFVDDPSSYCEGQLMERILSGKIPSCTLDDVRNFADTSIFWGSDPSSTQPRHLSRFS
                     YFPRGEKRQKSYEEERTCIVVDVRESPTARLCPNTTYRVLPGEDLQFLEAILAVLEGK
                     IPKFGDKKKMIELGSIIKKTEYGVIFPGPLMLVSLHDQMDRFEELLRRLNEITTFKVV
                     PMTGQYNSRGYSQLLFDRTGRVKGVKFAASGEAAEAPSLVEAVKGADAILVLGSDPLL
                     DLPLGLARSFAAAPIIAIDPRRSLTSDVARVVIPSAISGLETGGTALRTDGVRVTFEP
                     SMKTNQLADEQILTRIMEAI"
     gene            1509..1895
                     /locus_tag="PLV49_06160"
     CDS             1509..1895
                     /locus_tag="PLV49_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOG98463.1"
                     /translation="MAVDVKVVTYRDIFQYGAEKMGLHSEEYLRLSASIILDKGDMAS
                     LGIAEGGKVLLENELNRVVLNATASADKPHPGLAFMIKSPWSNQLEGDESCPAGASGF
                     RGIQAQISPSSESITQMDEILQRLRA"
     gene            1926..>2383
                     /locus_tag="PLV49_06165"
     CDS             1926..>2383
                     /locus_tag="PLV49_06165"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719395.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOG98464.1"
                     /translation="MNLISIADLSREDILRLIQSAEAFRVRRGRHGQPLEGKSLAMIF
                     EKPSTRTRVSLEVAAWELGGHPLYLSANELQLGRGETIADTARVLSRYVHGITARVHS
                     HGTVLQLAEHASVPVINALSDWEHPLQILADLQTIRQNFQALDGLQIGWIG"
BASE COUNT          473 a          662 c          772 g          476 t
ORIGIN      
        1 gagaccggcc ttgtcattca tccggagaag tgttttggct gcggcaactg cgtgatcgcc
       61 tgtccggtga atgtggcggc cgagcccacc agggtcggcg caggcctcgg tccggaaggg
      121 gatcgtgtca tcctccgggt ggaggatgga gtggtggtgg ccaataacct gcaagaatgc
      181 cggcgcttcg ggccgaatag ggtcttatgc aatgcctgta cggcgacctg tccgagcaag
      241 gccatagaat tcgtatgagg tgatgcagat gacagaattc aattacaaga cctgcaccgg
      301 ctgctcgctc atatgcgagg atatactgtt tccggacgct ggctctggat ccgtctccca
      361 ggccaagaac ctctgtcgca aaggccaggc tcactatcag gccctcttca gcgagaggac
      421 tgggcctctg gtggagggca gtgaggtttc tttagatcag gcgatagaaa gggcggcgga
      481 gattttaagg aacgccagcg cgcccgtgat ctacggctgg tccaactgca ccctggaggc
      541 gcagggggta gggctcgccc tggcggcgaa gatcggggcg ttcgttgatg atccttccag
      601 ttactgcgag gggcagctca tggagaggat cctctccggg aagattccca gctgcactct
      661 ggatgacgtg cgcaacttcg ccgatacctc catcttctgg ggttcggatc catccagcac
      721 ccagcccagg catctgtccc ggttctccta tttcccccga ggcgagaaaa ggcagaagag
      781 ctacgaggag gagaggacct gtatcgtggt ggatgtgagg gagtcgccca ccgcccggct
      841 ttgtcccaac accacctacc gggtcttgcc gggcgaggat ctgcagttcc tggaggcgat
      901 cctggcggtg ctggagggga agatccccaa gttcggggac aagaagaaga tgattgagct
      961 tggctccata atcaagaaga ccgagtacgg ggtgatcttc cccggtcctc ttatgctcgt
     1021 ctccctgcat gatcagatgg atcggttcga ggaactcctt cgccggctga atgagatcac
     1081 cacattcaag gtggttccca tgaccggcca gtacaactcc cgcggataca gccagctatt
     1141 gttcgaccgg acaggaaggg tcaaaggggt caagttcgct gcctctggag aggcggcgga
     1201 agcccccagc ctggtggagg cggtgaaggg agcggatgcc attttggttc tgggctctga
     1261 tcccctgctg gatctgcctc ttggcctggc gaggtctttt gctgctgccc ccatcatcgc
     1321 cattgatccc cgccggtccc tgacctcaga tgttgcccgg gtggtgattc cttctgccat
     1381 ctccggtctg gagaccgggg gcacggcgct gagaacggat ggggtgcggg tcaccttcga
     1441 gccctcaatg aagaccaacc aacttgcgga tgaacagatt ttgaccagga tcatggaggc
     1501 gatttgaaat ggcagtggat gtgaaggttg tcacctaccg ggatatcttc cagtacgggg
     1561 cggaaaagat gggattgcac tcggaggagt atctccggct cagtgccagc atcatcctgg
     1621 acaaggggga tatggccagt ctggggatag cagagggggg gaaggttctg ctggaaaacg
     1681 agctgaaccg ggttgtgctg aatgccacag cttctgccga taagccccat ccgggtctgg
     1741 cgttcatgat aaaaagcccc tggtccaatc agctggaggg ggatgagtcc tgcccggccg
     1801 gtgcttcggg gttcaggggg atccaggcac agatatcccc cagcagtgag agcatcaccc
     1861 agatggatga gatcctgcag aggttgcgtg cctagggcaa gggccccgga aaggaggatt
     1921 ctgacatgaa tctgatctcc attgccgacc tctctcgaga ggatatcctg cggctgatcc
     1981 agagcgcgga ggccttccgg gtcaggcgcg gccggcatgg ccagccgctg gagggaaaga
     2041 gcctggccat gatcttcgag aagccctcca ccaggacccg ggtcagcctg gaggtggcag
     2101 cctgggagct gggcggccat cccctatacc tatcggccaa cgaattgcag ctgggccggg
     2161 gggagaccat agccgatacg gcccgggtgc tgtccagata tgtgcatggc atcaccgccc
     2221 gtgtccactc tcacggcact gtgctccagc tggcggagca cgccagcgtc ccggtgatca
     2281 atgccctgtc cgactgggag catcccctgc agatattggc cgacctccag accattcgcc
     2341 agaacttcca ggctctggat ggcctgcaga tcggctggat tgg
//
LOCUS       DAOSGP010000217         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_44404_length_2355_cov_18.487826, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000217 DAOSGP010000000
VERSION     DAOSGP010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44404_length_2355_cov_18.487826"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLV49_06170"
     CDS             <1..945
                     /locus_tag="PLV49_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG98465.1"
                     /translation="ETIAVDNIDLQIRKGELFGLLGANGAGKSTIIKMLTTMLTPTSG
                     EARVWGYDIVRERNEVRSSIGVVFQDPALDGMLTGRENLDFHGRMYGMNAALRKARIA
                     DVLELVDLKEKADTKMQDYSGGMKRRLEIARGLMHRPHVLFLDEPTIGLDAQTRRYIW
                     EYIGRMNKEMNVTIILTTHYMEEADYLCDRVAIMDSGRIVALDRPESLKDTIGSDTIT
                     LMTDSGGDALVRSLQSFSWIKSIRSDNGSIKLHVDHAQSRIAEVVLEASRNNAVITSV
                     SLHKPTLEDVFIKYTGKKIKENGGKDRGADIALPRGGR"
     gene            942..1727
                     /locus_tag="PLV49_06175"
     CDS             942..1727
                     /locus_tag="PLV49_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98466.1"
                     /translation="MIADKVKGDLYAIYSIWLREMLRFFRLKSRLFGSVASPFFFLAF
                     LGIGFNNIGRIPGIPEGVDYMSFLTPGIIGMTLLFSATFAGLSVLWDREFGFLKEIMV
                     SPVSRIAIVLGRTAGGLTTGILQALIIMLSGILLGMGIPSIQGLILSLVFMILIATTF
                     IGLGLAFASKMEDMSGFSLIMNFLIFPLFFLSGALYPLDQFPEIIKILAYFNPLTYGV
                     DGLRYCLIGISAFSPLTDFAVLGVCCAAMLFLGAYLFETTEVN"
     gene            complement(1803..>2355)
                     /locus_tag="PLV49_06180"
     CDS             complement(1803..>2355)
                     /locus_tag="PLV49_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98467.1"
                     /translation="LAVPDEAVGRIVSVISADSLGIEMVINDPRTTGVDSVKLADIAA
                     PSTVTPEGKTARKAADLLLKDQIVYIDIDENSTEGRNEWGQMVCVIYLIDSRYRPIWP
                     PVNRMLVDDGHADLAEDGNNEFNSSAWWEDPIPQGSKGNISKYKEIMTSPKASSESQN
                     SASKSSVLDMESNAGRVSIGYRA"
BASE COUNT          514 a          643 c          632 g          566 t
ORIGIN      
        1 gagacgatcg ctgtagacaa tatcgacctt cagatcagaa agggggagct cttcggcctc
       61 ctgggtgcca acggagcggg gaagagcacc attatcaaga tgctcaccac catgctcacc
      121 cccacctcag gcgaggccag ggtttggggt tatgacatcg tccgggagag gaacgaggtg
      181 cgatcctcta tcggggtcgt ctttcaggat ccagcactgg atggcatgct caccggcagg
      241 gagaacctgg actttcacgg caggatgtac ggaatgaacg ctgccctcag aaaagccaga
      301 atcgccgatg tcctggagct ggtcgacctg aaggagaagg cagacaccaa gatgcaggac
      361 tactccggtg ggatgaaacg ccggctggag atcgcccggg ggctgatgca ccgccctcat
      421 gtcctatttc tagatgagcc caccatcggc cttgatgccc aaaccaggcg atacatctgg
      481 gagtacatcg gccgcatgaa caaagagatg aatgtgacta ttatcctcac cactcattac
      541 atggaggagg cggactatct gtgcgatcgg gtggccatca tggacagcgg caggatcgtg
      601 gccctggaca ggccggagag cctcaaggat accatcggtt cagataccat aaccctgatg
      661 actgatagcg gcggggatgc tctcgtccgg tctttgcagt cattctcctg gatcaagtcc
      721 atccgctcgg ataatggaag catcaagctt catgttgatc atgcccagtc tagaatagca
      781 gaagtggtac tggaagcgag caggaacaat gcggtcataa cctctgtcag tctgcacaaa
      841 ccaacactgg aggatgtatt catcaaatac actggcaaga agataaaaga gaacggcggc
      901 aaagatcggg gtgcagatat agctctcccc agaggcggca gatgatcgca gacaaggtca
      961 aaggcgatct gtacgccatc tacagcatct ggctgcggga gatgctgcgc ttcttccggc
     1021 tcaagagcag gctgttcggc tcggttgctt ctccattctt cttcctggca tttctgggga
     1081 ttggctttaa caatatcggc agaattcctg gaattcccga aggagtggac tacatgagct
     1141 ttctcacccc gggaatcata ggcatgaccc tgctcttctc ggcgacattt gccggcctat
     1201 ctgtcctctg ggaccgggag tttggcttct tgaaggagat aatggtctcg ccggtgagca
     1261 ggatagcgat agtgctggga cgcaccgccg gcggcctgac cactggcata cttcaggctc
     1321 tgatcataat gctctcgggg atactgctgg gtatgggcat tcccagcatc caggggctga
     1381 ttctatccct ggtctttatg attctcatag caacgacgtt catcggcctg ggcctggcct
     1441 ttgcgtccaa gatggaggat atgtccggat tcagcctgat catgaacttc ctgatattcc
     1501 cactcttctt cctctccgga gcgctctatc ccttagacca gttcccggag attatcaaga
     1561 tcctggctta tttcaatccc ctcacctatg gtgtggatgg actgagatac tgcctgatag
     1621 gcataagcgc cttctcgcca ctcactgatt ttgccgttct gggggtatgc tgtgcggcaa
     1681 tgctgttcct gggagcatat ctattcgaga ccactgaggt gaattgatca ggattagtca
     1741 accactgcct tctgaagggc gtggcagttg actaatctat gccgggcttg catctgcccg
     1801 gctcatgcgc gatagccgat gctgacccga ccggcgttgc tctccatgtc cagaacgctg
     1861 cttttgctcg cgctgttctg gctttcggat gatgcctttg gacttgtcat aatctcctta
     1921 tattttgata tgttcccctt cgatccctgg ggaatgggat cctcccacca ggcagatgag
     1981 ttaaactcat tattgccgtc ctccgcaaga tcggcatggc catcatccac tagcattctg
     2041 ttcaccggag gccatatcgg acggtagcgg gaatctatga gatagattac gcagaccatc
     2101 tgcccccatt cattgcggcc ttcagtgctg ttctcatcga tatcgatata aacgatctga
     2161 tctttcagca atagatcagc cgcttttcgg gccgtctttc cctcgggggt cactgtggat
     2221 ggagcggcaa tatccgccag cttcacgctg tccacccccg tcgtcctggg atcattgatc
     2281 accatctcta ttcccagtga gtctgcgctg atcacgctca caattcgccc tactgcttca
     2341 tcggggacgg ccaga
//
LOCUS       DAOSGP010000218         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_44551_length_2350_cov_14.206536, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000218 DAOSGP010000000
VERSION     DAOSGP010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44551_length_2350_cov_14.206536"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLV49_06185"
     CDS             complement(<1..637)
                     /locus_tag="PLV49_06185"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG98468.1"
                     /translation="MFGSKDHLTTIDNHLYIEDVDCCRLAAEEGTPLYVTSERRLREN
                     IRRYHRAFPDAEKYFAVKANGNLAILQVAAQEGMGADIFSAGELSLVRLAGIPRDMIL
                     FNGNSKSEKDHIAALQAGVRISVDSNEELEHLARTARNLDSEAEILFRVNPNVSPKTH
                     PKIATGLRSSKFGIPAEQVAGTYKRAMELEGVKPVGLHCHIGSQILDTAPFA"
     gene            complement(684..2246)
                     /locus_tag="PLV49_06190"
     CDS             complement(684..2246)
                     /locus_tag="PLV49_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOG98469.1"
                     /translation="MVLYIIVNSILLKQALKFASEMNGDLMRISDQVTQIFGPMSSNI
                     FSALIILVVGWIVALIVSSLIGKALRRTAMDEKIARMAVGEERARSIDSNRWITRIIY
                     YVLLFFVIVAFFETLGLTLVAQPLNEMLTVVFAYIPKLFAALLIAIVAIVLAMVLKRV
                     VLTVLRSANIDEDIGSKAGLERKEMPLSQTIAEIVFYLVLLLFLPMVLNALSLGGLLE
                     PLQVMMAKMLGFLPNLLAAAAILLVGWFLARIVQKILTGLLLAVGSERLSEKVGLSRV
                     LGERGLAGLIGLIVYALILIPVLMAALQALDLVSVTQPLSNMLNQILAALPSAFGAIL
                     ILAIAYVLGRIVADLVTNLLAAAGFDSILVWLGLGKEPAEGERKPSQVAGYLVLVAIM
                     LFALIEAFRILNFALMADLIYEFIVFAGRVLVGLVILATGIYLANLAYDTVMASNTSQ
                     AKMLAQAARISVLIFSGAMALRHMGLANEIINLAFGLLLGAIAVAVALAFGLGGKEIA
                     AREIDKWLKSMK"
BASE COUNT          579 a          638 c          610 g          523 t
ORIGIN      
        1 cggcgaaggg ggcggtgtcc agaatctggc tgccgatatg gcagtgcagt cccactggct
       61 ttaccccttc gagctccatc gctctcttat aggtcccggc cacctgctcc gcagggatgc
      121 cgaactttga ggatctcagg ccggtggcga tcttgggatg ggtttttggc gatacattgg
      181 ggttgactcg aaagaggatc tcagcctctg aatccaggtt acgggcggtc ctggccaggt
      241 gctccagctc ttcattggag tccacggaga tgcgaacccc tgcctggagg gcagctatgt
      301 gatccttctc gctcttggag ttgccgttga agaggatcat atccctgggg atgccagcca
      361 ggcgaaccag ggaaagctcc ccggcagaga atatgtccgc ccccattccc tcctgagcgg
      421 cgacttgcag aatggccaga ttgccattgg ccttcaccgc gaaatatttc tctgcatcag
      481 gaaatgctct atgatagcgg cggatgttct ctcgcaacct tctctcggag gtgacatata
      541 gtggagtgcc ctcctccgcg gccagacggc agcagtcgac gtcctcgata tagaggtggt
      601 tgtctatagt ggtgagatga tctttggatc caaacataga tcccatgatc tcttcttgat
      661 tactaaatcg tttctattaa agctcatttc atcgacttca accacttatc aatctccctg
      721 gcggcgatct cctttcctcc cagtccgaac gccagggcca cggccacagc gatggcgccc
      781 agcagcagtc cgaatgccag gttgatgatc tcattcgcca gtcccatgtg cctcagggcc
      841 atggctccgg agaatatcaa tacggaaatc ctggccgcct gagcgagcat cttcgcctgg
      901 ctggtgttgc tggccatcac tgtgtcataa gccagattgg ccaggtaaat gccggtggcc
      961 agtatcacca gtcccaccag aacgcggccg gcgaagacta tgaactcata gatcaggtcc
     1021 gccatcaggg cgaagttgag gattctgaat gcctctatca gggcgaagag cattatagcc
     1081 accagcacca ggtagcctgc cacctgagag ggctttctct ctccttctgc cggctccttt
     1141 cccaggccca gccagaccag tatggagtcg aatcccgctg ccgccaggag gtttgtgacc
     1201 agatctgcca caatccttcc cagcacataa gcgatggcca ggatcaagat ggccccgaag
     1261 gccgagggaa gggcagccag tatctggttg agcatattgg ataagggctg ggtgacggat
     1321 accaggtcca gggcctggag ggctgccatg aggacgggga tcagtatcaa ggcatagacg
     1381 atcaggccga tcaaaccggc cagccctcgt tctcccagta cccgggaaag gcccaccttc
     1441 tcgctcagcc tctccgagcc gactgccagc agcaggccgg tgaggatctt ttgcacgatg
     1501 cgggcgagaa accagccgac gaggaggata gctgctgctg ccagcagatt ggggaggaag
     1561 ccaagcatct tggccatcat cacctgcagg ggctcgagaa gcccgcccaa agagagtgca
     1621 ttgaggacca tgggcaggaa gaggaggagc acaaggtaga atacgatctc ggcaatggtc
     1681 tggctcaatg gcatctcctt tctctccagc ccggccttgc tgcctatgtc ctcatcgatg
     1741 tttgcagatc gaagaactgt caataccacc ctcttgagaa ccatcgctaa gacgatagcg
     1801 acgatcgcta tcaatagggc ggcaaagagc ttgggaatat aggcgaagac caccgttagc
     1861 atctcattta atggctgagc aaccagtgtt aagcccagag tctcgaaaaa tgccactata
     1921 acaaaaaata gtagaacata ataaattatc cgagtaatcc atcgattcga gtcaatggat
     1981 ctggcccttt cctcgcccac tgccatccgg gctatttttt catccattgc agtgcgacgc
     2041 agcgctttgc caatgagaga gctgacaatc aaagctacga tccaccccac aacaaggatg
     2101 atcaaagcac taaagatatt ggaactcata ggtccaaaga tctgagttac ctgatcgctt
     2161 attctcataa gatcaccatt catttcagat gcaaatttca aagcctgttt gagcaaaatg
     2221 ctattaacaa taatgtacaa taccattact taaagatagt ggatgattag cgcccggata
     2281 ttggatctca agaataaaac cggcacttta aagactagtc tgtaattaat ttttacaaaa
     2341 atgagtgata
//
LOCUS       DAOSGP010000219         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_44606_length_2348_cov_19.655473, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000219 DAOSGP010000000
VERSION     DAOSGP010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44606_length_2348_cov_19.655473"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /gene="cbiB"
                     /locus_tag="PLV49_06195"
     CDS             complement(<1..749)
                     /gene="cbiB"
                     /locus_tag="PLV49_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOG98470.1"
                     /translation="MTAIEQALAVFLLAVAFDILIGEPPVRIHPVVWIGRLISFLRAR
                     AGPTKVQGMLLALTVIAVSVLAAHLLVRAAGVLPVLPLLVGAYLLKSTFAIRCLLETS
                     SNIGRMIEQDIEVAKQMLPALVGRDTSSLRPAQASSAVIESLSENYVDSILSPIFYYI
                     LFTPLGLGLEAAIAFKAISTMDSMIGYKKPGLKELGYAGARLDDLANWIPARLSIILI
                     ALARPRGALSAIRAALKYHSATPSPNSGWPMA"
     gene            complement(976..2070)
                     /locus_tag="PLV49_06200"
     CDS             complement(976..2070)
                     /locus_tag="PLV49_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG98471.1"
                     /translation="MKNRLRKSFATAAECKHGGKVQEARLKLGFEPLDFSANINPLGS
                     PPLKELVSRELKQIGHYPDSSYQGFRRAASRFVQVDPGCIVPGNGSSELIRIFAEAVL
                     EEGDLAMIPTPTFGEYENQSHLAGGRTERLAIGGDGLPIIRDSDLRRAKALFLCNPNN
                     PTGRLLSQNQVHDLADRCEEAEVFLLVDEAFIELSRPEESVAQLAPSREYLFVMRSLT
                     KSFGVPGMRLGFGVTNPLLAGIMNRAKIPWSIGSIAAAAAEYLLGCEDHLTRSRELIA
                     EELNYLIPSLKNLGLDPLPSQVNFILVNIESTGLGSDTLAERTLDSGVLVRDCQSFGL
                     KKSYIRVAVRGHEENERLIDVLERALRCKD"
BASE COUNT          532 a          643 c          649 g          524 t
ORIGIN      
        1 gccatgggcc agccggagtt ggggctgggg gtggcgctat gatatttgag tgctgctctt
       61 atagcggaga gggcgcctct gggtcgggcc agggcgatca ggatgatgct taatcgagcg
      121 ggaatccagt tcgccaggtc gtccagcctc gctccagcat atccaagctc cttcaatccg
      181 ggctttttgt agccgatcat cgagtccatg gtgctgatgg ccttgaaggc aatggccgcc
      241 tccaggccca ggcccagagg agtgaagaga atatagtaga atatggggga cagaatggag
      301 tccacatagt tctcagatag cgactctatc actgctgagc tggcctgggc cgggcggagg
      361 gaggatgtgt cccttccaac cagggcaggc agcatctgtt ttgccacttc aatatcctgc
      421 tctatcatcc ggccgatatt gctggaggtc tccaacaggc atctgatggc aaatgtggat
      481 ttcaagagat atgcccccac caagaggggc agcacgggca gaactcctgc cgccctgacc
      541 agcagatgag ccgccagaac ggagactgca atcacggtta aggccagcag catgccctgc
      601 accttagtgg gcccggcccg agcacgcaag aaggagatca gtctgccgat ccagaccacg
      661 gggtgaatgc gaaccggagg ctcgccgatc agtatatcaa aggccaccgc taaaagaaaa
      721 accgccagag cctgttcgat agcggtcata gccgctcctc gaggctcttc cagacctggg
      781 agatggtctg accttgcaag agcccatcca gcaccatttc cgccacctct ttttggtgaa
      841 cgatctggca attctcacag caccaggagc catctcgctc tctgcctcct gttctcacat
      901 cctggcaggg gtaaaagggg cagaagcata atgagcagtc ctgctcgggg tggtggcagg
      961 gaaacctctc gcagctcaat ctttgcatct taatgccctc tccaggacat cgatgagccg
     1021 ctcgttctcc tcgtggcctc tcactgccac ccggatgtag ctctttttca ggccgaagga
     1081 ctggcagtcc cgcaccagaa ctccactgtc caatgtgcgc tctgccaggg tatcagagcc
     1141 aaggcccgtc gattcgatat tcaccaggat gaagttgacc tgacttggca gcggatccag
     1201 gcccagattc ttcagggagg ggatcagata attcagctcc tcggctatca gctctctaga
     1261 cctggtcagg tgatcctcac atcccaggag atattctgct gcagcggcgg ctatggagcc
     1321 gatggaccaa gggatctttg cccggttcat gattccagct aaaaggggat tggtcacgcc
     1381 aaagcccaat ctcatccccg gcactccaaa ggacttggtc agggatctca tcacaaagag
     1441 atactccctg ctgggggcaa gctgagctac gctctcctcc gggcggctga gctctatgaa
     1501 ggcctcatcc accagcaaga atacctctgc ctcctcgcat ctgtccgcca gatcgtgaac
     1561 ctgattttgg gagagcagcc gtccggtggg attattggga ttgcagagga acagggcctt
     1621 ggcccggcgc aggtcggagt cgcgaatgat tggaaggcca tctcctccaa tcgccagcct
     1681 ttcggtcctg cctccggcga gatgtgactg attctcatac tctccaaagg tgggggtggg
     1741 aatcatagcc aggtccccct cttccagaac agcctccgcg aagatcctga tcagttcaga
     1801 tgagccattg cctggaacga tgcatcccgg atcgacctgg acgaagcggc tcgctgcgcg
     1861 acggaatccc tggtagctgc tgtcggggta gtggccgatc tgtttgagtt ctcggctgac
     1921 caactccttc agggggggcg atcccagggg gttgatgtta gcggagaaat ccagcggctc
     1981 aaatcccagt ttcagcctcg cttcctgcac cttgccgcca tgcttgcact cggcagcagt
     2041 ggcaaagctc ttccgcaatc gatttttcat aggtcgttgt ggccttcaat gtattggcat
     2101 ttcaagcttg tgggcagagg acactccatt aacaatagtg atcttatgga gtcgctgtta
     2161 actgataatt tatttataaa attaatacta tgatgtttaa tcaaaattaa taaaattcta
     2221 tattaactgg tatccgaaag tgttttatct actctatgcg aactcaagcg gcgtatatca
     2281 aaggaagagg aggctaagat tccgatggca acaagagaag gcagcttttc ggttgaggac
     2341 atgaagaa
//
LOCUS       DAOSGP010000220         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_45725_length_2309_cov_20.228926, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000220 DAOSGP010000000
VERSION     DAOSGP010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45725_length_2309_cov_20.228926"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>2309
                     /locus_tag="PLV49_06205"
     CDS             78..>2309
                     /locus_tag="PLV49_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOG98472.1"
                     /translation="MIRLPELLAPAGSPQALHSAIAAGADAVYLSGKRFGARKFAANF
                     DHKAMTEAIDYAHLRGVKVYVTVNTLIREDELLDLAEYLVQLYELGADAILLQDLGAA
                     SLARRLVPDLERHASTQMTIHNLPGALYAAQGGFNRVVLAREVTIEEIKRWGQKMEKA
                     GLGLEVFVHGALCYSYSGQCLLSSAIGGRSGNRGMCAQPCRKPYVLMHGRKDEYGRPA
                     GLQAVAQKESYIISTRDLCVYRHLDKIVRSPVQSLKIEGRMKSPQYVAIVTSIYRRAL
                     DAIARGSWSPSKEEERDLALAFNRDFTSGHLLGAKDVMGRGMSDNQGLLIGSVASYDP
                     QRGEAAVRLCASLVPEKGDGLVFQSPGQEMGLVVQKAIEKDGLVRLRTPDRVRPGARV
                     FLTGSTALARRAEEIAARERASIPVNIRLTWEDGIPVAEGRLDSGELAVARGESRMGE
                     AVNRPLTPEQIESQMRKTGGTPFVARRVEMDYPGGLFSPPGALNQLRRDLLAGLEKAL
                     LERRRPPSDRIREAKDRLQKLELPARRAGEEWSRPKLSVYADRLDILKGAVDGGSRRI
                     YFEPLMEKGRGYKDRKETILAQLLEAKELCGKAELIWKWPRITRDDFIDLALPLLSEV
                     QVNGIMVENVGPAWAIKEKMPEMPIYGGSGLNVWNHLTAQALAPQFCWLTLSPELSRH
                     QLKDAVSAIRALPESDAPPNLELVVQGSLEVMVAEDSIPQLAKGSYSDELWGLQDFKH
                     IFPL"
BASE COUNT          500 a          650 c          725 g          434 t
ORIGIN      
        1 agatgatcat tgaggctcct cttttacggt aaggataggg cttatcaact caggggcaac
       61 agtcattctg actgacattg ataagactgc ctgaactcct cgcccctgct ggatcgcccc
      121 aggccctgca ctctgccata gcggcaggtg cggatgcggt gtacctgagc ggcaagaggt
      181 ttggagcgag gaagttcgct gctaacttcg accacaaggc catgacggag gccatagact
      241 acgcccacct gcgtggggtg aaagtttacg ttaccgtcaa taccctgatc agggaggatg
      301 agctgctcga tttagccgag tacctcgtcc agctgtacga gctgggtgca gatgccattc
      361 ttctccagga cctgggcgca gcctcactgg cccgccggct tgttccagat ctggagaggc
      421 atgcctccac tcagatgacc attcacaatc tccctggtgc tctctatgct gcccagggtg
      481 gcttcaatag ggtcgtcctg gccagagagg tcactataga agagattaaa agatggggac
      541 agaagatgga aaaggcggga ctgggcctgg aggtattcgt gcacggagcc ctctgctact
      601 cttactctgg ccagtgcctt ctctcctcgg ccatcggcgg gcggagcggc aacaggggga
      661 tgtgcgccca accctgcagg aaaccgtatg tcctgatgca cggcagaaag gacgaatacg
      721 gccggccggc gggcctccag gccgtcgccc agaaggagag ctatatcatc tccactcgcg
      781 acctttgcgt ctacaggcat ctggataaga tagtccgctc gcccgtgcag tcactgaaga
      841 tcgaagggag gatgaagtcc ccccagtatg tggccatcgt caccagcatc taccggaggg
      901 cactggatgc catcgcccgc ggcagctggt cgccctcgaa agaggaggag agggacctcg
      961 ccctggcatt caaccgcgac ttcacatcag gccacctgct gggggcaaag gatgtcatgg
     1021 gcagagggat gtcggacaat caggggctcc tcataggaag cgtggcctcc tatgatcccc
     1081 agagaggcga ggcagcagtc cgtctttgcg cttcacttgt gccagaaaaa ggggacggcc
     1141 tggtattcca gtctccgggc caggagatgg gcctggtagt gcagaaggcc atcgaaaagg
     1201 acggcctggt acgactcagg accccggata gagtccgtcc tggggccaga gtatttctca
     1261 ccggcagcac tgccttggcc cggagggcag aggagatcgc agccagggag agggcttcta
     1321 ttcccgtgaa catccgcctc acctgggagg acggaattcc agttgccgaa ggccggctgg
     1381 atagcggcga gctggcggtg gcccgaggag aatccaggat gggggaggct gtaaataggc
     1441 ccctgacccc agagcagatc gagtctcaga tgagaaagac tggaggcacc ccctttgtgg
     1501 ccaggagggt agagatggac tatcctggtg ggctcttctc tcctccggga gccttaaacc
     1561 agctgcgccg cgatcttctg gccgggctgg agaaggcatt actggagaga cgccggccac
     1621 catctgaccg gatcagggag gcgaaggatc gcctccagaa gctcgagctt ccggcgagaa
     1681 gggctgggga ggaatggagc aggcctaaat tgtccgtcta tgccgaccgc ctggatatat
     1741 tgaagggtgc agtagacggc ggatctcgtc ggatatactt cgagcccctg atggaaaaag
     1801 ggcgaggata taaggataga aaagagacga tcctggccca gcttctggag gcaaaagagc
     1861 tatgcggcaa ggcagagctg atctggaagt ggccacgaat cactcgggat gatttcattg
     1921 accttgccct gcccctgctc tccgaggtcc aggtgaacgg gatcatggtg gagaacgtcg
     1981 gccctgcctg ggcgatcaaa gagaagatgc ccgaaatgcc gatttatggc ggatccgggc
     2041 tgaacgtatg gaaccacctg acggcccagg cccttgcacc ccagttctgc tggctcaccc
     2101 tctcccccga gctatcccgc caccagctga aggatgcagt ctccgccatc cgggctttgc
     2161 ccgagagtga cgcccccccc aatctggagc tggtcgtcca gggcagcctg gaggtgatgg
     2221 tggccgagga ctcgatcccc caactggcta aaggaagcta ttctgatgag ctctggggcc
     2281 tgcaggattt caagcacatc tttcccctg
//
LOCUS       DAOSGP010000221         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_46509_length_2282_cov_19.688819, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000221 DAOSGP010000000
VERSION     DAOSGP010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46509_length_2282_cov_19.688819"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="PLV49_06210"
     CDS             complement(<1..1457)
                     /locus_tag="PLV49_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOG98473.1"
                     /translation="MSWLSLVFAIVLAMALVSLGGAQQSLPAQKILSQIAAGQSVHYD
                     GIAITGDLDLSSLPGAQVPAAFALFNCTIPDASFEGITFAEDAFFWGSSFGNASFQGA
                     TFLGRADFANTSFRQASFAGCTFYQPALFGGALFENDLSFEDTAFGLDASFQAVRFLG
                     KVDFNYSDFDSYSYFAAAEFAEDAFFSDVDLSATSDFSAATFSGQANFIRSRLNEPYF
                     GDVLFSGPAQFGMARFSGLTSFGGAVFADEAGFGLARFSDAAYFSGAHFQDLALFGLA
                     RFEDIVTFQDTRFDGDLNFKSGSITSLLFDEAELKEGSRIILNDTEINLFRAHWKEIQ
                     DHVAWQPGAYLALVENYRRLGWLEDEDDCYYQYRKLDQASKGWGWSKIIDILAWLSCG
                     YGVRPSYAVVWSLVTILIFGLVFWIGDGIRRSSKPLSGPVEKDSIPERATLRNALFFS
                     TMIFLSQGPIDFLPMGRHRYYVMLEMILGWLLLAL"
     gene            complement(1547..>2282)
                     /locus_tag="PLV49_06215"
     CDS             complement(1547..>2282)
                     /locus_tag="PLV49_06215"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HOG98474.1"
                     /translation="TINNKLLAIILAALNLALLGCAYGSIDSSLNGSIQAQSEPLDTS
                     GALALSDLDEWMFYENYDYGFELIYPADWIEQEAEANDQGMIVGFLAPGEDVDNPTVY
                     LLLQNEELPAGQDVTLEVYSQATLGVLNDIMPDLQILTESDITINEVPAHAIVYSLES
                     EGVTFKVLKAWTVIDENAYIFTYNAPDELYDQFAEDAADIIDSFDAGLFARIPESSGP
                     LEEAAENESLNETSKLMEEENTSVVQ"
BASE COUNT          556 a          621 c          632 g          473 t
ORIGIN      
        1 aacagcgcca agagcagcca gcccaatatc atctccagca tcacgtagta cctgtgcctg
       61 cccatgggca gaaagtcgat ggggccctgg gagaggaaga tcatggtgct gaaaaagagt
      121 gcatttcgca atgtggcccg ctcggggatg ctgtctttct ccaccggccc gctcaggggt
      181 ttggacgagc gacggatgcc gtcccctatc cagaagacca ggccgaagat cagaatagta
      241 accaaagacc ataccacggc atagcttggc cgcactccat agccacagga gagccaggcc
      301 aggatatcta tgatctttga ccagccccag cccttgctgg cctgatctag ctttcgatac
      361 tgatagtagc agtcgtcctc atcttccagc cagcccagcc ggcggtagtt ctccaccagg
      421 gccagatatg ctcccggctg ccaggccaca tgatcctgga tctccttcca gtgagcccgg
      481 aagaggttga tctcagtatc gtttaggatg atgcgggagc cctcttttag ctctgcctcg
      541 tcgaagagca gagaggtgat ggatccggat ttgaagttga ggtcgccatc gaaccgggta
      601 tcctggaagg ttacgatgtc ctcgaacctg gccaggccga agagggccag gtcctgaaag
      661 tgggcgccgg agaagtaggc ggcatcggaa aatctggcca ggccgaagcc cgcctcatcg
      721 gcgaataccg ccccgccaaa ggaggtgagg ccggaaaaac gagccatgcc aaactgggca
      781 gggccggaga ataggacgtc cccgaaatag ggctcattca ggcgggagcg gatgaagttg
      841 gcctggccgg agaatgtggc agcagagaag tccgaggtag cggaaaggtc gacatcggag
      901 aagaaggcat cttctgcgaa ctctgctgct gcgaagtagg agtaggaatc gaaatcggag
      961 tagttgaaat caacctttcc caggaagcgg acggcttgga aggaggcatc caggccgaag
     1021 gcggtatcct caaagctcaa atcattctca aacagcgctc caccgaagag cgccggctgg
     1081 tagaaggtgc agccagcaaa actggcctgg cggaaagagg tattggcaaa gtccgcccgg
     1141 cccaggaatg tggccccctg gaagctggca ttgccaaagg agcttcccca gaagaaggca
     1201 tcctctgcaa atgtaattcc ctcaaaggag gcgtcaggga tggtgcagtt gaaaagtgcg
     1261 aatgctgccg gaacctgtgc tccgggcaat gaggatagat cgagatcgcc agtgatggca
     1321 atgccatcgt aatggaccga ttggcccgcc gcaatctggg ataggatctt ctgggcgggc
     1381 agagactgct gggcgccgcc cagggatacc aatgccatcg ccagaacaat ggcgaatacg
     1441 agagacagcc aggacatcta ccggccttca tgcaagttcc tggacatatt ctttgcgcag
     1501 atcgccatat tgccgatttt ttcgcctcat atcccccttt tccatcctac tggacaacgc
     1561 tggtgttctc ctcctccatc agcttcgacg tctcattcag gctctcattt tcagccgctt
     1621 cctccaatgg tcctgagctc tcagggattc gagcaaataa gccagcatca aaggagtcaa
     1681 tgatatcggc ggcatcttcg gcaaactgat cataaagctc atcaggtgcg ttgtatgtga
     1741 atatgtatgc attctcgtca atcacagtcc aggccttcaa gactttgaat gtcacaccct
     1801 cgctttccag gctatacact atggcatgag ccggcacttc attgattgtg atatcgctct
     1861 cggtcaggat ctggagatct ggcatgatgt cattgagaac tcccaacgtt gcctggctat
     1921 acacttccag ggttacatcc tgccccgcgg gaagctcttc gttctggagc aacagatata
     1981 ccgtcggatt atcgacatcc tctccagggg ccaggaagcc cacgatcatt ccttgatcat
     2041 tggcttcggc ctcctgctcg atccaatcag ccgggtaaat cagctcaaat ccatagtcgt
     2101 agttctcata aaacatccat tcatccaggt cggagagggc cagggccccg gaggtgtcca
     2161 gaggctcgct ctgggcctga atgctgccat ttaaagagga gtcaatagaa ccgtaagcac
     2221 aaccaagcag cgccagattc agtgctgcta gaattatggc tagaagctta ttattgatcg
     2281 ta
//
LOCUS       DAOSGP010000222         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_46579_length_2280_cov_11.870112, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000222 DAOSGP010000000
VERSION     DAOSGP010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46579_length_2280_cov_11.870112"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(682..1089)
                     /locus_tag="PLV49_06220"
     CDS             complement(682..1089)
                     /locus_tag="PLV49_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1699 family protein"
                     /protein_id="HOG98475.1"
                     /translation="MKLRVVSSKKEITDLNRNEQLVHLAFRASNMDVMNLVQSCPRLR
                     AVQIPPSYHETMSRAAQQFLEIQGVQLLKGDVWGHRKDIDEYYAIGEQIVNRISTLMS
                     EGNSIEETVRKLQRETKLSEDLIRYILKEQVMA"
     gene            complement(1340..>2280)
                     /locus_tag="PLV49_06225"
     CDS             complement(1340..>2280)
                     /locus_tag="PLV49_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HOG98476.1"
                     /translation="GSYPLPRGVRLDGLSRDRYLQLVGQVLAEKIRAGVEVPTYPQLR
                     DMIRMFMDPIQDPDMTESPFLIRREKAEILELQAVPPGQKVRVCVTGPVELYISAFAA
                     THYTDILYTLAESVSRFLQWAKDEGKMAVASLDEPSLGLNSNIIFSEEEIQEALEIAS
                     RPCQGMHCEVHLHSPLYAELCAHVSGIDILGVESAAHPDYLKLIEREVLEDTGTYLRA
                     GIARTDIFSLSAQLNDRYKVNLWEDLPRLEREIISLESPLIMKSRLDKAYAQFGDRLI
                     ATGPDCGLGPWPSQELAGSILGNCAAAIASFRSEHP"
BASE COUNT          569 a          588 c          494 g          629 t
ORIGIN      
        1 tcataatcca tcgactccag gttcttaagt ccttggagca agatgggctt ggcctcttct
       61 tttccgatgt aaccaatgat cccgaccatt cccaatcatc caagattaat acattttatc
      121 aatattttta tatataatca ttattaactt ttcgaaaagc cattgatcag ccatggagcg
      181 aatcatacta tcaccggatt tcctcgcgag gggagattta attccttcgc ctcaatcatg
      241 atatgaactc aattccatcc tgcgccgcat atgacaagtc gatctatcta tttcaattat
      301 ttaattttgt tgcttgtttc ttttgtttct agcttcgcta attaaatcaa acactcacat
      361 tatcttgaat ttatagaaat aaataattgc cctgcaaatg atatacactc gctttacgcc
      421 ttcgcgcatg ttcatctgat ccttctgatc cctgccatgg agactgaacg cacaattcat
      481 ctgttgcttt gcgatggaga atatgcccga aatgtgggtg gagaatgcgc gcgccattcc
      541 tccaggctct gcatgagaaa actcccgctc tcatatcccc cttcttcctc agagcctatg
      601 ctcttctggc aggcagatct attttaaaaa agagttatgc ttcgagctgg ggtttattgg
      661 atcccgtcgg tttggatgca atcaggccat tacctgctct tttaagatat agcgaatgag
      721 atcctctgag agcttggtct ctctttgcag cttcctcaca gtttcttcaa tgctgtttcc
      781 ttcggacatg agggtggaga tccggttcac tatctgctct ccaatggcat aatactcgtc
      841 gatgtccttg cggtggcccc atacatctcc cttcaggagc tgaactccct gaatctccag
      901 gaactgctga gcggcccggg acatggtctc atggtaagat ggtggtatct gaacggcgcg
      961 cagacgggga catgactgca ccagattcat cacatccatg ttagaggccc tgaaagctag
     1021 atgaaccaac tgctcattcc tgttcagatc tgtgatctcc ttttttgaac ttactaccct
     1081 cagcttcatg atacatacca atgctcttaa aatatttata gataatggta atcatagtac
     1141 tccataatta gatatactga gatcagaaat tgaattagga aaatatttat gctcaggcct
     1201 ttgccatagc aaaagagctc agataaatgt taaataattc tatatgtttt tatgataaga
     1261 aacaatttat attttcataa cggcatttgc tttccaaata ttgaaggttc aatactccaa
     1321 tcaaagattg cttgaatgat cagggatgct cagagcgaaa tgaagcaata gctgcagcgc
     1381 agttgcccag aatgcttccg gccagctcct ggcttggcca gggccccaat ccacagtccg
     1441 ggccagtggc aattagccta tcgccgaact gggcataggc cttatccagc ctgctcttca
     1501 tgataagagg cgattccagg ctgatgatct ccctttccag acggggcaga tcttcccata
     1561 gatttacttt atatctatca ttaagctggg cagatagaga gaagatatcg gtgcgagcga
     1621 ttcctgccct cagatatgtc ccggtatcct ctaggacctc cctctcgatc agcttcaggt
     1681 aatcaggatg agccgctgac tcgactccta agatgtctat ccccgagacg tgcgcgcaca
     1741 gctcggcata gagaggtgag tgcagatgca cctcgcagtg cattccctga caggggcggc
     1801 tggcgatctc cagagcctcc tgaatctctt cctcagagaa gatgatattg gagttcaggc
     1861 ccaagctcgg ctcgtccagt gaggcgacgg ccatctttcc ctcatccttt gcccattgca
     1921 ggaacctgga aacgctctcc gccagggtat aaagaatatc agtgtaatgc gtcgccgcaa
     1981 aagcggatat gtataactca accggtcccg tgacgcagac ccgcaccttc tgaccgggag
     2041 ggacggcctg tagctccagt atctccgcct tttctctcct gatcagaaag gggctttcag
     2101 tcatatccgg atcctggatg gggtccatga acatgcgaat catatcccgg agctgaggat
     2161 aggtgggaac ctccaccccg gcgcgaatct tctccgccag aacctggcct acgagctgca
     2221 gatatcgatc cctggacagg ccatccagcc taactcccct gggcagggga tagctgccga
//
LOCUS       DAOSGP010000223         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_47122_length_2261_cov_18.696736, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000223 DAOSGP010000000
VERSION     DAOSGP010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47122_length_2261_cov_18.696736"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2261)
                     /gene="polC"
                     /locus_tag="PLV49_06230"
     CDS             complement(<1..>2261)
                     /gene="polC"
                     /locus_tag="PLV49_06230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HOG98477.1"
                     /translation="RNTGLAAAGINPASMLLLGEFMAAGTQIKVEQPGKAAAVAPVSS
                     IEGPTVRLLNGDVVRFDSDQDILEWMPISCKDPKTIQIALKAHVSRILDLGEILISYG
                     EFLENNRPLAPASYAFEWWAAQLLKAGGDPTGCESMYGEEAIALSREYSVPLHPAHTH
                     LWHDLSIEDYELLREVAASKGILESGRLILPLEAKESLETLLVLHKVRDGRIIIEDPL
                     PFLLCLGIEGEGGSLKKRTEEAGGEQGGGDQAEDSTLTRVNRLSGIVVRARAPTRIGA
                     RMGRPEKSDKRLMRPPPHVLFPAGEEGGKGRSIQEAAKHSTNSSSGIINVQIERRVCK
                     SCGKEGFAFICECGSHTEKKRVCPKCNIPAPEKCPKCGQETNAVAEMQIDVKKLYHLA
                     LESLGEREPESLKGVLGLSSRDKTPEPLEKGILRAKHGINIFKDGTVRYDLTDLPLTH
                     FRPDEIGTSPEVLQSLGYAVDMHGDPLTRPDQVCELRIQDIVLSRDAGEYLLKVSWFT
                     DELLAKFYHLEPFYNAQTPQDLIGKLMVGLAPHTSAGVLCRLIGYSPASAGFGHPFFH
                     AAKRRNCDGDEDCVMLLMDALINFSMSYLPAKRGGKMDACLVMTTILNPAEVDKEAHN
                     LDLTPVYPLEFYEATLTNTSPKDLEGRFDLVSKRLGTDQQYEGFNFSHATTDIAAGPK
                     NSAYKTLETMIDKMDAQLELARMIRAVDEQDVAERVINSHFLPDLIGNLHAFSKQKVR
                     CVKCGAKYRRPPL"
BASE COUNT          423 a          710 c          633 g          495 t
ORIGIN      
        1 ttcagtggag gccttctata cttggctccg cacttcacgc acctcacctt ctgcttggag
       61 aaggcatgca gattgccaat aaggtcgggc aggaaatgag agttgatcac ccgctctgct
      121 acgtcctgct cgtccaccgc ccggatcatc cgcgccagct ccagctgggc atccatctta
      181 tcgatcatgg tctccagggt cttgtaggcg ctgttcttcg gcccggcggc gatgtccgtg
      241 gtggcatggc tgaagttgaa cccctcatac tgctggtcgg tgcccagccg cttggacacc
      301 agatcgaacc tcccctccag atccttgggg ctggtgttgg tgagggtggc ctcgtagaac
      361 tccaggggat agaccggagt cagatccagg ttatgagcct ccttgtccac ctccgccggg
      421 ttgagaatgg tggtcatgac caggcaggcg tccatcttcc ctcccctttt cgcgggaaga
      481 taggacatgg agaagttgat cagggcatcc atgagcagca tgacgcaatc ctcgtcccca
      541 tcgcagtttc tccgctttgc ggcatggaag aatggatggc caaacccagc cgaggccggg
      601 ctgtagccga tcagtcggca gaggacgcct gcagaggtat ggggggccag gcccaccatc
      661 agctttccga tcagatcctg gggggtttgg gcattataga atggttccag atgatagaac
      721 ttggccagca gctcgtctgt aaaccaggat accttcagga ggtattcgcc cgcatcccga
      781 gataggacga tatcctggat cctcagctcg cagacctgat ccggacgggt taaaggatcg
      841 ccatgcatat ccacggcata gcccaggctc tgcagaactt ccgggctggt gccgatctcg
      901 tccggccgga aatgggtgag gggcagatcg gtgaggtcgt acctcactgt gccgtccttg
      961 aagatgttga tcccgtgctt ggcccgcagg attcccttct ccaggggctc aggggtcttg
     1021 tcccgtgagg agaggcctaa aacccctttc agcgactcgg gctccctctc ccccagactc
     1081 tcaagcgcta ggtggtagag ctttttgaca tcgatctgca tctctgctac cgcattggtc
     1141 tcctggccgc atttgggaca cttctcagga gcggggatat tgcatttggg gcagaccctc
     1201 ttcttctcag tatggctgcc gcactcgcag atgaaggcga atccctcctt tccgcagctc
     1261 ttgcacaccc gccgctcgat ctgcacattg atgatcccgg aagagctgtt agtggagtgc
     1321 tttgccgcct cctgaatgga ccggcccttg cctccctcct cccccgcggg gaagaggaca
     1381 tgaggcggcg gcctcatcag cctcttgtca gacttctcgg gccggcccat gcgggctccg
     1441 atgcgggtgg gcgcccgggc ccgaaccaca atgccagaga gtcggttgac cctggtcaga
     1501 gtcgagtcct ctgcctgatc acccccgccc tgctcgcccc cagcctcctc ggtcctcttc
     1561 ttcaatgaac caccctcccc ctcgattccc agacacagca ggaagggtag agggtcctcg
     1621 attattatcc ttccatctcg gaccttgtgc agcaccagca gggtctccaa tgactccttg
     1681 gcctccagag gtaaaatcag ccgtcccgac tccagaatgc cttttgatgc cgcaacctcc
     1741 cgcaatagct cgtaatcctc tatggagagg tcatgccaca ggtgggtatg agcaggatga
     1801 aggggaacgc tatattctct ggacaaggca atagcctctt cgccatacat agactcacag
     1861 cccgtggggt cgccgcccgc ttttagtagc tgggccgccc accactcaaa tgcatatgag
     1921 gccggtgcca ggggccggtt gttctccaga aactccccat agctgatcag gatctccccc
     1981 aggtccagga tcctggatac atgggccttg agggcgatct ggatggtctt gggatctttg
     2041 cagctgatcg gcatccactc cagtatatcc tggtcggagt caaagcgcac cacatccccg
     2101 ttgagcaacc gcactgtggg cccctcaatc gagctgactg gcgctaccgc cgccgctttg
     2161 cccggctgct ccaccttgat ctgggttccg gcggccatga actcccccaa aaggagcatg
     2221 ctggccggat tgatccccgc agcagcaagc ccggtgttcc t
//
LOCUS       DAOSGP010000224         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_47193_length_2259_cov_18.252722, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000224 DAOSGP010000000
VERSION     DAOSGP010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47193_length_2259_cov_18.252722"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLV49_06235"
     CDS             complement(<1..533)
                     /locus_tag="PLV49_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG98478.1"
                     /translation="MRADKMRPRVFLLKAGSILRDQAGNILDARSSVTLIESEKERII
                     VDTGQEGDGEVILKALADLGLEGSDIDIIVNTHSHPDHCANNRLFSRAKTVYPKDGEL
                     IAPGVRVLATPGHSPDSISVLVDAAIHPPGGDGMASATRRVVIAGDALPTLGNFQKRV
                     PPAVHYDRALAVASMNK"
     gene            complement(581..1909)
                     /gene="purB"
                     /locus_tag="PLV49_06240"
     CDS             complement(581..1909)
                     /gene="purB"
                     /locus_tag="PLV49_06240"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOG98479.1"
                     /translation="MEFRYGTREMKAIWEEDFRLRCLFRVEAALAQAEEELGMIPPGA
                     AEEIARAAEVTLPQRAKEIEDEIGHDMMATVLALAEESPRFGEWIHLGATSNDILDTA
                     TGLQIKASLDILEEKLKKMLALLLDLAMQNRNLVCAGRTHGQIAVPTTYGLRFAIWAS
                     EVGRHIQRLEELRPRAAVGKMSGAVGTQAAYGRFGMEIQERVMHHLELFEVDVSNQVV
                     QRDRHAEMVCWMALVASTLDKIFIEMRTLQRTEIAEVEEGFGKKQVGSSTMPHKRNPI
                     KSEQICGLARVVRAQLIPAFENIPLWDERDLTNSSCERVVFPEAFIFTDHILALAVRT
                     LSNMRLRPDNIERNLMLLHGLNMAENVMVELAKKGVGRQQAHEIMRQSSMTAFEEKRE
                     LKDVLLENETVRSFLKPEEISALLDPHQYIGTAPKQVERLNEKLKKLYLV"
     gene            complement(1994..>2259)
                     /locus_tag="PLV49_06245"
     CDS             complement(1994..>2259)
                     /locus_tag="PLV49_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98480.1"
                     /translation="DVVVAGAPSGKKTGYVNIIGRNGNRASEPVRVDQPALEDAEEGE
                     FQEPETEPLAQPDKPAESQKESPAPGALAASLVLILALWRMKR"
BASE COUNT          419 a          705 c          606 g          529 t
ORIGIN      
        1 atcttgttca tgctggccac ggccagggcg cgatcgtagt gcactgccgg aggaaccctc
       61 ttttggaagt tgcccagagt gggcagggcg tcccctgcta tcaccactct ccgggtcgcc
      121 gatgccattc catctcctcc gggaggatgg atggcagcat cgaccagcac ggatatgctg
      181 tccgggctgt ggccgggcgt ggccaggacc cgaacgccgg gggcgattag ctctccatct
      241 ttgggatata ccgtcttagc ccgagaaaac agccggttgt tggcgcagtg gtcgggatga
      301 gaatgggtgt ttacaattat gtcgatatca gacccctcca gccccagatc ggccagagcc
      361 ttgagtatca cctctccatc cccctcctgg ccggtgtcaa ctatgatcct ttccttctcg
      421 ctctcaatca gcgtcactga ggagcgggca tccaggatgt tgcccgcttg atccctcagg
      481 atcgaccccg ctttgaggag aaagaccctg ggccgcattt tatcagctct catatcatct
      541 gcgtccaaat catcccggcc caatccagcc tggctcacgc tcaaaccagg tagagctttt
      601 tcagcttctc gttcagcctt tccacctgtt tgggagcagt gccgatgtac tgatgaggat
      661 caagcagggc gctgatctcc tcaggcttca ggaaacttct gaccgtctcg ttctccagga
      721 ggacgtcctt caattccctt ttctcttcga aggcagtcat gctgctctgg cgcatgatct
      781 catgggcctg ctggcggccc actccttttt tggccagctc caccatcacg ttctccgcca
      841 tgttcaggcc atgcaggagc atgaggtttc gctcgatgtt atccggcctc agcctcatat
      901 tgctcagggt gcgaacggcc agggcgagga tgtggtcggt gaatatgaag gcctccggaa
      961 agacaacccg ctcgcagctg ctgttggtca gatcgcgctc gtcccagaga ggaatgttct
     1021 caaaagcggg gatgagctgc gcccgtacca cccttgccag gccgcagatc tgctcggact
     1081 tgatcggatt tctcttgtgg ggcatggtgg acgagcccac ctgctttttg ccaaagccct
     1141 cttccacctc ggcgatctcc gtcctctgca aggtcctcat ctcgatgaat atcttatcca
     1201 gggtggaggc gacgagcgcc atccagcata ccatctctgc atgcctgtcc ctctgcacca
     1261 cctgattgga gacgtccacc tcgaacagct ccaggtgatg cataaccctc tcctgaatct
     1321 ccatgccgaa ccggccatag gcggcctggg tccccacggc gccagacatc ttgcccactg
     1381 ccgcccgggg tcgcaactcc tcgagcctct ggatatggcg cccaacctca gaggcccaga
     1441 tggcgaacct caatccgtag gtggtgggca ctgctatctg gccgtgcgtc cggccggcgc
     1501 agaccaaatt tctgttctgc atggccaggt ccaggagaag agcgagcatt tttttcagct
     1561 tctcctccag gatatccagt gaagccttga tctgcagtcc ggtagcggta tccaggatat
     1621 cattggaggt tgcccccagg tggatccact ccccaaacct ggggctctcc tcggccaggg
     1681 cgagcacagt ggccatcatg tcatggccga tctcatcctc aatctccttg gccctctggg
     1741 gcagggtgac ctctgcggcc cgagcgatct cctctgccgc cccgggggga atcattccca
     1801 gctcctcttc cgcttgagcc agggccgcct ccacccggaa gagacagcgc aatctgaaat
     1861 cctcttccca gatggccttc atctctcgtg tgccatatct gaactccatg gggtgaatag
     1921 acatgccagg aaggatggca gaggggattg atggagcttt tggtctggga gacaatagaa
     1981 atatctatat tttttatctc ttcatcctcc aaagggcgag gatcaacaca aggcttgcgg
     2041 ccaatgcccc tggagctggg ctctccttct ggctttcagc gggcttgtcg ggctgggcaa
     2101 gaggttccgt ctcaggctct tggaattctc cttcttctgc atcctccagt gcgggctgat
     2161 ccactctcac aggctcactg gcacgattgc catttcttcc aatgatgttg acatagccgg
     2221 tcttctttcc ggaaggggca ccagcgacga ccacatctg
//
LOCUS       DAOSGP010000225         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_47391_length_2253_cov_20.540946, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000225 DAOSGP010000000
VERSION     DAOSGP010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47391_length_2253_cov_20.540946"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..>2253
                     /gene="glgP"
                     /locus_tag="PLV49_06250"
     CDS             110..>2253
                     /gene="glgP"
                     /locus_tag="PLV49_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOG98481.1"
                     /translation="MTTDRVWELLDPEAWEKTKNPYLILQNISKSRLNELAQDDALRT
                     EIKDWQEKNAKFLAQPGWSEEHFNLGSVAYFSMEFGLSEALPIYSGGLGILAGDHLKT
                     ASDLGVPLVGVGLLYQQGYFRQVLAQDGSQIEAFPYNDPNSLPIEPVQDEEGGRLRIK
                     ISLPGRHLYLRVWRAQVGRVNLFLLDSNDPLNSPWDRAITASLYAPGQELRLIQEIIL
                     GVGGWLAIKKMGIKPIVCHMNEGHAAFVVLARAYSFMHEEGCSFLEALWATRAGNVFT
                     THTPVEAGFDRFDRDILKKYAKHYTDAQGLSPNDLVIMGLKTEKDDSSPFNIALLALS
                     GSCFVNGVSRLHAQVSKRIFQSRYPRWPENEVPIDYITNGVHIPTWDSPVAQRLWSLS
                     CPTGCGLDSIDYEAGKIARQSDQDIWNFRALARKDLIDYARRRHARQMQEHGASPEEI
                     EAARHVLDPNTLTIGFARRATAYKRTNLLLRDEEKLVSILTNHDRPVQLLMAAKAHPA
                     DVAGKEMVKQMANFADRPDVTDRVIFLEDYDIDLAQHLQAGVDLWINTPLRPNEACGT
                     SGMKVLANGGLNLSTLDGWWDEAYEPRVGWLLGDEGDEIEPPAGFGPERDDLDARNLY
                     RLLEEEVIPLFYQRDDQGVPSSWVAKIKASMTELTPRYSGNRMMKEYVEKVYQPAADA
                     YRKRAKEGAKLAKELVGWQKRINQCWRGVHFG"
BASE COUNT          555 a          559 c          669 g          470 t
ORIGIN      
        1 aatggaaatt gatcgaggcg gataaatgaa aagccatacg gacctgcaat tgccctcagg
       61 actggaaggt cttgctgatc tggccttaga tttgcgctgg acagttcggt tgacgacaga
      121 tagggtctgg gagctgctgg atcccgaggc ctgggagaag accaaaaatc cctatctcat
      181 cctgcaaaac atctcaaaaa gccgactaaa cgagctggca caagacgatg ctctgagaac
      241 ggagataaaa gattggcagg agaaaaacgc gaaatttttg gcacagccag gatggagcga
      301 ggagcatttc aatctgggct ctgtggccta cttcagtatg gagttcggcc tttctgaggc
      361 ccttcccatc tactctggag gcctgggaat cctggctgga gaccacctga agacggccag
      421 cgacctggga gtcccactgg tcggagtagg cctcctctac cagcagggat acttccgtca
      481 ggttttggcc caggacggat ctcagatcga ggcctttcct tataacgatc caaacagcct
      541 tcccatagag ccggtccagg acgaggaggg aggcaggctc agaatcaaga tcagccttcc
      601 ggggagacat ctgtacttaa gggtctggag ggctcaggtg ggccgggtga acctctttct
      661 cctggactcc aacgatcctc taaacagccc atgggatagg gcgatcaccg ccagccttta
      721 tgctcccgga caggagctgc gcctcatcca ggagatcatc ctgggcgtag gcggttggct
      781 ggctataaaa aagatgggaa taaagcctat tgtctgccat atgaacgagg gccatgctgc
      841 atttgtagtc ctggccaggg cctatagctt catgcatgag gaaggctgct cattcctgga
      901 ggctctctgg gccacccggg cgggaaatgt attcaccact cataccccgg tggaggcggg
      961 tttcgatcga ttcgatagag atattttgaa aaaatatgca aagcattata cagacgctca
     1021 aggcctctcc ccgaatgatc tggtcataat gggattgaag accgaaaagg atgacagtag
     1081 cccctttaac atcgctcttc tcgctcttag cgggagctgc tttgttaacg gagtgagtcg
     1141 gcttcatgcc caggtgagca agaggatctt tcagtcccgc tatccccgct ggccggagaa
     1201 tgaggtgcca attgattaca ttaccaacgg ggttcatatt cctacctggg attctcctgt
     1261 agcccagaga ttatggtcct tgagctgtcc gacgggatgc ggtctggaca gcatcgatta
     1321 cgaggcagga aagatagcca ggcagagcga ccaggatatc tggaacttca gagctcttgc
     1381 cagaaaagac ctaatcgatt atgctcgcag gcgtcacgcc cggcagatgc aggagcatgg
     1441 agcctcgcct gaggagatag aggccgcaag gcacgtcttg gaccccaata ccctcaccat
     1501 aggctttgcc aggagggcaa ccgcctacaa gcgcacaaat cttctcctca gggacgagga
     1561 gaagctggtc agtattctga ccaatcatga taggccggtt cagctcttga tggctgcaaa
     1621 ggctcatcct gcagatgtcg ctgggaagga gatggtcaag cagatggcca acttcgccga
     1681 tcggccggat gttacggacc gggtgatatt tctcgaggat tacgatatcg atctggccca
     1741 gcatctgcag gcgggagtgg atttatggat taacactcca ctccgtccca atgaggcctg
     1801 cgggacgagc ggcatgaagg ttctggccaa tggcgggctg aacctctcca ctctcgatgg
     1861 atggtgggat gaggcctatg aaccacgggt gggctggctg ctgggagatg agggagatga
     1921 gattgagccc cctgcaggct ttgggccgga aagagatgat ttagatgccc ggaaccttta
     1981 ccgtctgctg gaggaggagg taataccgct cttctaccag cgggacgatc agggagttcc
     2041 atcatcctgg gtggcaaaga tcaaggccag catgactgag ctaaccccgc gctatagcgg
     2101 caaccgaatg atgaaagagt atgtagagaa ggtctaccag ccagcggctg acgcctatag
     2161 aaagagggca aaagagggag caaagctggc gaaggagctg gtgggttggc agaagcggat
     2221 caaccaatgc tggagaggcg ttcattttgg cca
//
LOCUS       DAOSGP010000226         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_47392_length_2253_cov_19.589627, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000226 DAOSGP010000000
VERSION     DAOSGP010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47392_length_2253_cov_19.589627"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1645)
                     /locus_tag="PLV49_06255"
     CDS             complement(95..1645)
                     /locus_tag="PLV49_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOG98482.1"
                     /translation="MAVKDGVGGLSKPGSNPPHFLILLVSIIYLLALLSISLSISPAL
                     ADEAQAPDFFATSWDQRSFNLSDFMGSPVVLHITNIENPLCIECEKSLSGQVEELAAL
                     KALDTNVQIVTLNLRKNPYSIDGRELAQTWWEVNITWPWIEDLDPYPIGSKYLDYWTV
                     RGGSSNPTIVLIDREGRIGPVYHVYRVGEGIEDGVQKAQSLLDDIQELNGTALMVEGE
                     ASPPAEVEARPEGAAGIFSALWTRMENEVSRKDVTAFGMFLLGIFTSLAPCSIALMIA
                     VFSYVMTVRRKDEYLRASASTSREGFMIGVAFTLGMAVVFFVLGLFISQIGVFFRDSK
                     LFDLLAGVIMVLLGISSFKPLGEIIEPVMDRIRLHRAAMDGLDEPSESSGLNGPGEEK
                     KSLLQKAVEFSLELFRYSAFIGAFTLGIFFALGWAPCALSMVMPVLIWLASQDVAPLV
                     GGTMLFFFGIGHGVPIIPITTFSRMVGGRIGEKYVSVGEWTSKIFGLLVILVGLVYAA
                     RYFGYLLW"
     gene            complement(1654..2226)
                     /locus_tag="PLV49_06260"
     CDS             complement(1654..2226)
                     /locus_tag="PLV49_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG98483.1"
                     /translation="MLDRFTNFLTLLIASLLTISLFCAVALAEDEDDNGPEHSLGSGD
                     SDWWTTYPDKNVNSGSAVNHPDWVIEALEDRPLLILIHQNNCLPCVTHVPRINDAIKI
                     HNDDILFYSILAEGDGILKAMEVLDIYSPTGKKQYVPTTIFLTLIENEDGEVEVGWHS
                     EIDIMSTDDINSYVEDSIYYHQQNAADWEQ"
BASE COUNT          527 a          635 c          601 g          490 t
ORIGIN      
        1 gcaatctcct ggaggctttc cagcatggtc gaaatgtaat ctcaccttcc tctcgatatc
       61 ctcacactgt aattactgcg ccgttattgt acccttacca taacaggtat ccaaaatacc
      121 tggcggcata gaccaggccc accaggatca ccagcagccc gaagatcttg ctggtccact
      181 ctcccaccga tacatacttc tccccgatcc tgccgcccac cattcgggag aaggtggtga
      241 tggggatgat gggcactcca tggccgatgc cgaagaagaa gagcatcgtc ccgccgacca
      301 ggggggcaac gtcctggctt gccagccaga tcaggaccgg catcaccatg gagagggcac
      361 agggagccca acccagggcg aagaagatcc ccagggtgaa ggcgccgatg aacgcagaat
      421 agcggaagag ctctagcgag aactccactg ccttttggag caggctcttc ttctcttccc
      481 ctggcccatt taagcccgag ctctcggacg gctcatcgag cccatccatg gcagcacgat
      541 gcaggcggat gcgatccatg accggctcga tgatctcccc caggggcttg aagctgctga
      601 ttccaagcag gaccatgatt accccggcta gaaggtcaaa gagctttgaa tcccggaaga
      661 agacccctat ctgggatatg aacaggccga gcacaaagaa gacaaccgcc atgccgaggg
      721 tgaaggcgac gccgatcatg aatccctctc ttgaggtgga ggcgctggct cgcaggtatt
      781 cgtccttcct gcgaacggtc atcacataag agaacacagc gatcatcagg gcgatggagc
      841 agggggcaag ggaggtgaag atgcccagaa ggaacatgcc aaaggcggtg acatccttcc
      901 ttgagacctc attctccatc ctggtccaga gggcggagaa gatgcctgct gccccctctg
      961 ggcgggcctc cacctctgca ggaggggagg cttccccctc caccataaga gctgtcccat
     1021 tgagctcctg gatgtcatcc agaaggctct gggccttctg caccccatct tctatccctt
     1081 cacccacccg gtagacgtgg tagaccgggc cgattctgcc ctctctgtcg atcagaacaa
     1141 tggtgggatt ggaagagccg cctctcaccg tccaatagtc caggtatttg ctccctatgg
     1201 gatatgggtc cagatcctcg atccagggcc aggttatatt gacctcccac caggtttggg
     1261 ccagctcccg gccgtcaatg gaataggggt tcttcctcag gttcagggtt actatctgga
     1321 cattggtgtc tagggccttg agggctgcaa gctcctctac ctgccccgag agggactttt
     1381 cgcattcaat gcatagcggg ttctcgatat ttgttatatg caggaccacc ggggagccca
     1441 tgaaatcgga tagattgaag ctcctttgat cccagctggt tgcgaaaaag tccggcgcct
     1501 gcgcttcatc tgctagcgcg ggactgatgg atagggatat ggacaatagc gccaataaat
     1561 atattatgga aaccagcaaa atgaggaagt gagggggatt agagcccggc ttgctcagac
     1621 cacccacccc atctttcact gccattaggc cgatcactgc tcccagtcag ccgcattctg
     1681 ctggtgataa taaatggaat cctctacata gctgttgata tcgtccgtgc tcatgatatc
     1741 aatctcgcta tgccagccaa cctcaacctc gccgtcctcg ttctcgatca gagtgaggaa
     1801 gatggtggtg ggaacatact gcttcttgcc tgtgggactg tagatatcca gaacctccat
     1861 tgccttcagg attccgtctc cctcagccag aatgctgtag aacaggatat cgtcgttgtg
     1921 gatttttata gcatcattta tcctggggac atgggtcacg cagggcaggc agttgttctg
     1981 gtggatcagg atcagcaggg gcctgtcctc cagagcctca ataacccaat ccgggtgatt
     2041 tacggcggag ccagagttga cgtttttgtc cgggtatgtc gtccaccaat cagaatcacc
     2101 cgagccaaga gagtgctcag gaccattgtc atcctcatcc tctgccaggg caacggcaca
     2161 gaaaagagag atggtcaaaa gagaggcgat caatagtgtg aggaaatttg tgaaccgatc
     2221 aagcatgatt taaacctccg aattcgtcct ctt
//
LOCUS       DAOSGP010000227         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_47509_length_2249_cov_17.089790, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000227 DAOSGP010000000
VERSION     DAOSGP010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47509_length_2249_cov_17.089790"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2157
                     /locus_tag="PLV49_06265"
     CDS             <1..2157
                     /locus_tag="PLV49_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HOG98484.1"
                     /translation="LVFGFLLKLFAGRNSLTEHGIVFPGYDEYYHMRRILYGTSNFPD
                     TLWFDTYLNYPHGFSITWPPLFDQISAALCIALGQHTKEGVEMTAAFVPVIIGLLAMV
                     VVYYIMRELFDHRIALLASFMTALAPYYLLYTMVAATDHHSLEVLFQLLSLLFMVLAF
                     SRPDKRWLYASAAGVTMAALAYTWQGADVYFGAYLLFAAVGISLELRQDKPNLNIVST
                     LLVAFFLALILVLPFRNTPWMSPSFLGLAAIFVALAIMLALAHIIAKRSISWKAFPLA
                     ILAIFIISLLLSKILGGFFGLEGLIRTGMGYIWGGEMIGKIGEAEPLIYDLDTLSQVV
                     FSWLGLNILFSLAGAAAIVVYMRKEERRMQQGLLLLLIWAVFSILLTLGQGRFLYIST
                     IFMGMLISILIFYALDLSKKFQEERGTKLNRALAAAVILLLVLPTAIDAFNFASNSPP
                     AIAGDWHDSLFWLKENSDPTSHFADPEEIPEYSVMSWWDYGNWILYLSERPVVANNFQ
                     TGIEDAVKFYLAEDEKEATEIMDARGARYVFADFGIAYGKLPSLTEWADEDISSYMAL
                     EEYGAQLSAKPKERFFNTTLGKLYLADGTGMGRFRLIHESSTIMGNVGKSRVMIFQYV
                     PGALLKIHTGRDGNGMALLNMTSNMDRRFVYLNQATWNGDALEVRVPYSTEREHEVHA
                     TDPYMVFSVHGDDVKAQMVEVSEEDVLQGRTIDINF"
BASE COUNT          513 a          594 c          588 g          554 t
ORIGIN      
        1 cttgtcttcg gcttcttgtt gaagctcttt gcaggaagga actcacttac cgaacatggg
       61 atagtgttcc caggctacga tgaatattat catatgcgca ggatactcta tgggaccagc
      121 aactttcctg atacgctatg gtttgatacc tacctgaact accctcatgg cttttccata
      181 acctggcccc cgctcttcga ccagatctcc gctgccctat gcatcgcttt gggccagcat
      241 acaaaagagg gtgtggagat gacggcggcc tttgtgcctg tcatcatcgg tctgctggct
      301 atggtcgtcg tttactatat catgcgagag ctcttcgatc acagaattgc ccttctggca
      361 agcttcatga ccgctctcgc cccctactac ctcctctata cgatggttgc cgccaccgac
      421 catcactctt tagaggtgtt gtttcagctc ctctctctcc tgttcatggt cctcgcattc
      481 tcccggccgg ataagagatg gctctacgct tctgcggcag gcgtgacaat ggccgccctg
      541 gcatatacct ggcagggcgc agatgtctac ttcggagcat atctgctatt cgcggcagtt
      601 gggatcagcc tggagctgcg ccaggataag ccaaacctga acatcgtctc aacgctgctt
      661 gtagcctttt tcctcgccct tatactcgtt ttgcccttca ggaatacccc ctggatgtca
      721 ccctccttcc tgggattggc agccatattc gtcgctctgg ccatcatgct ggcactggca
      781 cacattatcg ctaagaggag catatcctgg aaggcattcc cattggccat cctcgccata
      841 ttcattatat cattgctgct ctcaaagata ttgggcggat tctttggact ggagggtctg
      901 atccgaacgg gaatgggata catctgggga ggggagatga tcggcaagat tggagaggca
      961 gaaccgctca tctatgacct tgatacgtta tctcaggtgg tcttctcctg gctgggactt
     1021 aatatcctat tctccctggc aggagcagcc gccatagtag tctatatgcg aaaagaagaa
     1081 agaagaatgc agcagggcct gcttctcctt ctgatatggg cagtcttctc catccttctc
     1141 accctggggc agggcagatt tctgtacatc tccacgatat tcatgggcat gctgataagc
     1201 atactgatat tctatgccct ggatctctcc aagaagttcc aggaggagag gggcacaaag
     1261 ctcaaccggg ccctggcagc agccgtcatc ctgcttcttg tcctgccgac ggcaattgat
     1321 gccttcaact tcgccagcaa ttcgccacct gcgatcgcag gcgactggca tgactctctt
     1381 ttctggctga aggagaacag cgacccaacc agccactttg ccgatccgga agagatccct
     1441 gagtacagcg tgatgagctg gtgggactat ggcaactgga tcctctatct atctgaaagg
     1501 ccggtggtgg cgaacaactt ccagaccgga atagaggatg cggtgaagtt ctatctggca
     1561 gaggacgaga aggaagcgac ggagataatg gatgccagag gtgccaggta cgtctttgcc
     1621 gatttcggca ttgcctatgg caagctgccc tccctcacag aatgggcgga tgaggatatc
     1681 agcagctata tggccctgga ggagtacggc gctcagctct cagccaaacc caaagagagg
     1741 ttcttcaaca ccacattggg caagctctat ctggcagacg gaacaggcat gggccggttc
     1801 cgcctgatcc acgagtcgag cactatcatg ggaaatgtgg gaaagagcag ggttatgatc
     1861 ttccagtatg ttcccggagc gttgctgaaa atccacactg gccgggatgg aaatggcatg
     1921 gccttgctca atatgacctc caatatggat aggaggttcg tctacctcaa ccaggccacc
     1981 tggaatggag atgcacttga ggtaagggtc ccctattcca cagagagaga gcatgaggtc
     2041 catgcaactg acccgtatat ggtgttctca gtgcatggag acgatgtgaa ggcacagatg
     2101 gttgaggtga gcgaggagga tgttctccag ggaagaacca ttgatatcaa cttttaaaaa
     2161 agagaattgc gccgtttccg gcgcatcaat ttgatattct cgattagggc ttatacgcag
     2221 ggcagccaat caacgctgat ttcgccaca
//
LOCUS       DAOSGP010000228         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_48039_length_2232_cov_17.128158, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000228 DAOSGP010000000
VERSION     DAOSGP010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48039_length_2232_cov_17.128158"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLV49_06270"
     CDS             complement(<1..611)
                     /locus_tag="PLV49_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOG98485.1"
                     /translation="MTYEILEGENHAHRWPNLVSLALMLLVVLNVAAVIMETVEWLYF
                     AYGPIFEAFNVFSIGIFTIEYILRIWSCIEDPRYRAPQGSRLRFAITPLAVVDFIAVF
                     PFYLPFVIADLRFVRAMRLFRLFRILKLAHYSRALQTFDEVLRLKKEELGLMLFTIII
                     LLITSAGLMYEAEHDAQPEAFASIPDAMWWGIVTLATVGYGDI"
     gene            686..1420
                     /locus_tag="PLV49_06275"
     CDS             686..1420
                     /locus_tag="PLV49_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOG98486.1"
                     /translation="MRAALLQQTVLPCQDLKNLSQALFLASSALEMGAEILVYPELFL
                     SGFCYEPKDRFWEIGTASFEELDPFRALAIDYDCLIIGSVRRGRSNLGFCLDRTGLKL
                     RPKIHPFGEEKKHFDGGDSISPIATRWGRVGLEICYDLRFPEVARSLALQDADFLVTV
                     AQFPEQRREQWRALSLARAIENQIPHLACNWAEGGGSMIISALGTVLAGAESGEEIIF
                     GEVDLSERDQVRGEIPCFSDRRPEVY"
     gene            1445..>2232
                     /locus_tag="PLV49_06280"
     CDS             1445..>2232
                     /locus_tag="PLV49_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit PriS"
                     /protein_id="HOG98487.1"
                     /translation="MNFLISKFREYYIQATLDVPPGLQSREWGFLFFDDSGMRRHKSF
                     FSRGELVDYVRSMIPRHVYHSAAYYQRPGAPTMKEKIWQGADLIFDLDADHLRGAARS
                     YAGMLEQVKRETLKLLSFLLSDFGFAESKISVVFSGGRGYHIHVRDPRIYSFGSDERR
                     EVVDYLSGRGLVLDRFIHEAPVEGWGREGGRRLIAPARDSPGWGGRINRSIISFVEGL
                     RALDEPGAVAVLSRVRGIGPKKASSFYRSLEGEGVIEEIRRGNL"
BASE COUNT          461 a          627 c          669 g          475 t
ORIGIN      
        1 tagatatctc catacccgac agtggccagt gtcacaatgc cccaccacat ggcatcagga
       61 atggaggcga aggcctcggg ctgggcgtca tgctccgcct cgtacatcaa gccggcggag
      121 gtgatgagca gaatgattat ggtgaagagc ataagaccga gctcctcctt cttcagccgg
      181 aggacttcat cgaatgtctg cagggcgcgc gagtaatggg ccagcttcaa aatccggaag
      241 agacggaaga ggcgcatggc tcgaacgaac ctcagatcgg cgatgacgaa tggaagatag
      301 aagggaaaca ccgcgatgaa gtccaccact gccaggggag ttatcgcaaa gcgcagtctg
      361 ctgccctgag gggcgcggta tcttggatcc tcaatgcagc tccagatgcg caggatatac
      421 tcgatggtga atattcctat ggagaataca ttgaatgcct caaaaatggg tccataggcg
      481 aagtagagcc attctaccgt ctccatgatc accgctgcga cattgagaac cactaaaagc
      541 atgagggcga ggctgaccag atttggccat ctgtgcgcat ggttttcccc ctccaggatc
      601 tcatatgtca aacgccgata gcttttgggc atcctggccc agcttatgtc catcttaata
      661 tatacgaact tgcctctttt tcagcatgcg tgctgccctc cttcagcaga ccgtcctgcc
      721 ctgccaggat ctgaaaaacc tttctcaagc cctctttttg gccagctccg ctcttgagat
      781 gggtgcagag atactggtct atccggagct cttcctatcc ggattttgct atgagcctaa
      841 agatcgcttc tgggaaatag ggaccgcctc atttgaggag ctcgatccct tccgcgccct
      901 ggcaatagat tatgactgcc tgatcatcgg ctcagtccgt cgcggccggt caaacctcgg
      961 cttctgcctg gaccgcaccg gcctgaagct gcgccccaaa atccatccct tcggcgagga
     1021 gaagaagcac ttcgatgggg gggattcgat ctcccccata gccacccgct ggggccgggt
     1081 ggggctggag atctgctatg acctgcgctt tcccgaggtg gcccgctccc tggccctgca
     1141 ggatgctgac ttcctggtga ccgtggctca gtttcctgag cagcggcgcg agcagtggcg
     1201 ggcgctaagc ctcgcccggg cgatagagaa ccagatccct cacctggcct gcaactgggc
     1261 agaagggggt ggctccatga tcatcagcgc cctgggcact gtgctggcag gggcggagag
     1321 cggcgaagag atcatattcg gcgaggtcga tctatccgag agggaccagg tacgaggtga
     1381 gatcccctgc ttttccgacc gccggcccga ggtgtattga ggtgatggca taaaccccct
     1441 gaccatgaac ttcctcatat cgaagttccg ggagtattat atccaggcca ccctggatgt
     1501 ccctccaggc ctccagtcca gggaatgggg ttttctcttc ttcgatgact caggcatgcg
     1561 ccggcacaag tccttcttca gccgggggga gctggtggac tatgtgcgct ccatgattcc
     1621 ccgtcatgtc tatcattcag ccgcttatta ccagcggccc ggtgcaccga cgatgaagga
     1681 gaagatatgg cagggagcgg accttatatt cgatttggat gccgatcacc tgcgcggtgc
     1741 cgccagaagc tatgccggga tgctggagca ggtcaagagg gagaccctaa agctcctctc
     1801 ctttttgcta tccgatttcg gttttgcgga aagtaaaatt agcgtcgtct tctccggcgg
     1861 ccggggatat cacatccatg tccgggaccc gcggatctat agcttcggga gtgatgagcg
     1921 ccgggaggtg gtagactacc tctcaggcag gggcctagtt ttggaccgat tcattcacga
     1981 ggctccggtt gaaggatggg ggcgggaggg gggcaggcgt cttatagctc ctgctcggga
     2041 ttcgcctggc tgggggggaa ggatcaaccg ctcgatcatc tcctttgtgg aagggctccg
     2101 cgccctcgac gagcccggag cggtggccgt cctctcccgg gtccgaggca tcggccccaa
     2161 gaaagcctcc agcttctacc gcagcctaga gggtgagggg gtgatagagg agatccgccg
     2221 ggggaacctg ga
//
LOCUS       DAOSGP010000229         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_48141_length_2229_cov_21.032199, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000229 DAOSGP010000000
VERSION     DAOSGP010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48141_length_2229_cov_21.032199"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..912
                     /locus_tag="PLV49_06285"
     CDS             223..912
                     /locus_tag="PLV49_06285"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HOG98488.1"
                     /translation="MIQGHEIWLERSKIENDDVELSLVYGHNMRQDGVADISRFLPFV
                     FLPDGIRGEAVLSAGDDRHSMKFKAENAGHYTAFVDMGTTVWSQTKDGYNQGPKFQFK
                     DVIYAGAYNQMAKTIISVGNGSDYIGRPLHGILEIVPKEPFCRAGQEIELSVLYEEKP
                     LASADIKAVSKKEGREIGSARTDENGVARIPIDVDGECMFLVRHADGTKRVNEEFDET
                     VFVNTLVLESR"
     gene            909..1694
                     /locus_tag="PLV49_06290"
     CDS             909..1694
                     /locus_tag="PLV49_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG98489.1"
                     /translation="MKRIIFSGKGGVGKTTILSNLARLLARDGYRVLVIDCDPSMNLA
                     MSLGIPLSDVVSLAKDSSHLWERLGPQAEEEEHGEHGLECTEEDLDQFIIQAADGVKL
                     IVMGTIPFGGAGCLCAPISLVRLLVNYLASGPEEHDFIFVDSQAGVEIFGRGLASEFD
                     LSFVITEPTPKSLEVAKHGLKLAGDLGVKRQIVVVNKVEVDEDLQMAKRELDGNAQQI
                     MSVGYDREVVEADKKGALLLDYAPNSSSLRDIVRVEQFMLSEA"
     gene            1695..1895
                     /locus_tag="PLV49_06295"
     CDS             1695..1895
                     /locus_tag="PLV49_06295"
                     /inference="COORDINATES: protein motif:HMM:NF021636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HOG98490.1"
                     /translation="MCEFTVYLLGDNQKDRQMMAKDILVARRKEGKVLLMDAFGSITP
                     VENATIEEVNTLSQEMILKRMN"
BASE COUNT          568 a          482 c          650 g          529 t
ORIGIN      
        1 tgcggctttg catctgcgga tctgatcttc cgccttccat gttctctgat tggaggacca
       61 atccagagct cggggggtaa catcattttt gcttttaatt taatatatta ttactaaata
      121 ctattgataa aggaaaactt attaataaac aaaccctatt accccaatca atcacatcgg
      181 taatgaggaa tggagcatat gcaagagaga aatttgtcat tgatgattca gggccatgag
      241 atctggctgg agagatcgaa gatcgagaat gatgatgttg agctgtccct tgtctatggc
      301 cacaacatgc gccaggatgg cgtggctgat atcagcaggt ttttgccatt tgtctttctt
      361 cccgatggaa tccggggaga ggcagttcta tctgcgggcg atgatcgcca ttcaatgaag
      421 ttcaaggcag agaatgccgg ccactacacc gctttcgtcg acatgggcac gaccgtctgg
      481 tcgcagacca aggatggcta caaccagggt ccaaaattcc agttcaagga tgtgatctat
      541 gctggagctt acaaccagat ggccaagacc ataatctctg tgggaaatgg cagcgactac
      601 attggccgcc cattgcacgg gattctggag atagtgccaa aagagccctt ctgccgcgct
      661 gggcaggaga tcgagctctc cgtcctgtac gaagagaagc ctctagcgtc tgcagatatc
      721 aaagccgtct ccaagaaaga gggacgggag attggatccg ccagaacgga cgagaatggt
      781 gtggcaagga ttcccatcga cgtggatggc gaatgcatgt ttttagtcag gcatgcggac
      841 gggacgaaga gagtgaacga ggagttcgac gaaaccgtat ttgtcaatac gctggttctg
      901 gagtcccgat gaaacggata attttctcag gaaagggtgg tgtgggcaag accaccatcc
      961 tttccaatct ggccaggctg ctggctcggg atggctacag agtgctggtc atagactgcg
     1021 atccgagcat gaatctggcc atgtccctgg gcattcctct cagcgatgtc gtctccctgg
     1081 caaaggatag cagccatctt tgggaaaggc tcgggccgca ggcagaggaa gaggagcatg
     1141 gtgagcacgg attggagtgc acagaggagg acctcgatca gttcattatc caggcagcag
     1201 acggagtgaa gctgattgtg atggggacaa tacccttcgg cggcgcaggg tgcctgtgcg
     1261 ctcccatatc cctggtccgg ctgctggtga actacctggc atccggtccg gaagagcacg
     1321 acttcatctt cgtggactct caggcaggtg tggagatctt cggccgggga ctggcctcgg
     1381 aattcgacct ctccttcgtc atcactgagc ccacccccaa gtccctggag gtggcaaagc
     1441 acggcctgaa gctggctgga gatctggggg tcaagagaca gatagtggta gtgaacaagg
     1501 tggaggtcga tgaggacctt cagatggcaa aaagagagct ggacggaaat gctcagcaga
     1561 taatgtctgt gggctatgac cgggaggtgg tggaagcgga caagaaaggg gcattgctcc
     1621 tcgattatgc cccgaattcc agctccttga gggatattgt cagggtagag cagttcatgc
     1681 tttcggaggc ataaatgtgt gaattcacgg tatatctgtt gggcgataac caaaaggatc
     1741 gtcagatgat ggcgaaagac attctcgtcg ccaggagaaa ggaaggaaaa gtgctcttga
     1801 tggatgcttt cggcagcatt acaccggtgg agaatgccac tatcgaggag gtaaataccc
     1861 tttctcagga gatgatactg aagaggatga attagaaacg attgatcgcc ggattgaggg
     1921 cattgacgac tggggaatat tgggctttct aagccatccc cgttctctac tgaattaccg
     1981 gttgaacgac gatgcttatc aagtgtcaca aatattgtga taaatagata attctattgc
     2041 agattggatt ttttattatg atctggaaaa gatggatgat gagctcattg tggatcttac
     2101 tctttgccgt gtcctcggcg gatgcccttg atgtgcggct ggatgtcttt ggcaatgcca
     2161 acctggatga taccattgat gaagaggaca ttgcatatgt gaaggatata ctgaagggat
     2221 cggcggaga
//
LOCUS       DAOSGP010000230         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_48823_length_2208_cov_18.441245, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000230 DAOSGP010000000
VERSION     DAOSGP010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48823_length_2208_cov_18.441245"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1763
                     /gene="oadA"
                     /locus_tag="PLV49_06300"
     CDS             57..1763
                     /gene="oadA"
                     /locus_tag="PLV49_06300"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HOG98491.1"
                     /translation="MGRVMLTETVFRDAHQSLLATRMRTRDMLPIAEQMDQVGFFSLE
                     VWGGATFDSCIRYLNEDPWERLRALKKAMPNTPMQMLLRGQNLVGYRHYADDLVDRFV
                     KRAAENGVDIFRIFDAVNDIRNMERSIKAAKKMDKHVQAGISYTISPVHSNEQFAQFA
                     VQLAELGCDSICIKDMGGLITPKNAYDLIRAIKKEVNLPVNLHTHCTSGMAQMSYFHG
                     CQAGVDILDTAMSPLSGGSSQPATESLVAALKGTPYDTGLDLELLTEIKRYFERIMEI
                     YAPVFNAIAARVDTNVLVYQIPGGMLSNLVSQLQEQKAMDKYEEVLAEVPKVRKDLGY
                     PPLVTPTSQIVTTQAVLNVVTGDRYKMVPKEVKDYVKGLYGKSPAMIDPKVRIKVLCD
                     EDAVTVRPADLLPPEYEEAKKAVDALGLAKKEEDYLTYALYPQVALKFLKGAATEESL
                     VKKDMASDLGASLKAEPLALNVEIDGEAYLVKVAPAGMTIQEAQPKAPTDGVTVPMQG
                     VVIRYKVKKGDRVAKGDPVAVLEAMKMEQNILANKDGKIREIYVKEGTTVAPGDVLIS
                     IE"
     gene            1793..>2208
                     /locus_tag="PLV49_06305"
     CDS             1793..>2208
                     /locus_tag="PLV49_06305"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720091.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOG98492.1"
                     /translation="MTVKREQVLIEALPYIREFYDSIMVFKIGGSIMSDTAVLEDFIQ
                     DVVLLRYIGIHPIIVHGGGPEISQAMEKFGKKAEFVGGLRVTDRETLSIARMVLLGHI
                     NAELVNLIGKHGGKGIGLSGQDGMFLKAKKKALQMV"
BASE COUNT          556 a          566 c          640 g          446 t
ORIGIN      
        1 atgagattca ttaggcgaag ggattttaaa gagggaccaa ataagaggtg atctttatgg
       61 gcagggtaat gcttacagaa acggttttta gagatgctca tcagtctctg ctggccacca
      121 gaatgaggac cagggacatg ctacccatag cagagcagat ggaccaggtc ggcttcttct
      181 ctctggaggt ttggggagga gctaccttcg atagctgcat tcgctatctg aatgaggatc
      241 cctgggagag gttgagagct cttaagaagg ccatgcctaa cacccctatg cagatgctgc
      301 ttcgcgggca gaacctggtg ggctacaggc attatgcaga cgatttagtg gacagatttg
      361 tgaagcgagc agccgagaac ggggtcgata ttttcaggat tttcgatgcg gtcaatgata
      421 taagaaatat ggagcgatcg atcaaggctg ccaagaagat ggacaagcat gtccaggccg
      481 gaataagcta caccatcagc cccgtgcaca gcaatgagca atttgcccag ttcgccgtcc
      541 agctggcaga gcttggctgc gactccatct gcatcaaaga tatgggcggt ctgatcaccc
      601 ccaagaatgc ctatgacctc atccgggcca taaaaaaaga ggtcaacctt ccggtgaacc
      661 tgcacaccca ctgcaccagc ggcatggctc agatgagcta cttccatggt tgccaggcag
      721 gagtagatat cctggatact gccatgtctc ctctctccgg tggcagctca cagccggcta
      781 cagagagcct ggtggcagct cttaagggaa ccccttatga caccggcctg gatctggagc
      841 tcttgactga gatcaaaaga tacttcgaac ggatcatgga gatctatgcc ccggtattca
      901 atgccatcgc cgcccgggtg gacaccaatg tcctggtcta tcagattccg gggggcatgc
      961 tctccaacct ggtcagccag ctgcaggagc agaaggccat ggacaagtac gaggaggtgc
     1021 tggcagaggt gccaaaggtt cgcaaggatc tgggatatcc tcccctggtc actcccacca
     1081 gccagatagt gaccacccag gcggtcctga atgtggtcac aggcgaccgc tacaagatgg
     1141 ttcccaaaga ggtcaaggat tacgtcaaag gcctgtatgg aaagtcgccc gccatgatcg
     1201 accccaaggt caggatcaag gtcctgtgcg atgaggacgc agtcaccgta cggcctgcag
     1261 atctattgcc ccccgaatat gaggaggcaa agaaagcggt tgatgccctc ggcctcgcca
     1321 agaaggagga ggattacctc acatacgccc tctaccccca ggtggccctg aagttcctca
     1381 agggagcagc cacggaggaa tccctggtca aaaaagatat ggcatcggat ctgggagctt
     1441 ccctaaaagc cgagcccctg gcccttaatg tcgagatcga tggcgaggcc tatctggtca
     1501 aggtggctcc agcgggcatg accattcagg aagcccagcc caaggctccc actgatggcg
     1561 tgaccgttcc catgcagggc gtagtcatcc gctataaggt caagaagggc gaccgggtgg
     1621 ccaaagggga tccagtagca gtcctggaag cgatgaagat ggagcagaat atcctggcca
     1681 ataaggatgg caagataaga gagatctatg tcaaagaagg gactacagtc gctcccggcg
     1741 atgtcttgat atccattgag tagaggaacc caaaatccga gaggaatgaa tattgacagt
     1801 taagagagaa caggtgctca ttgaggctct gccttacatt cgggagttct acgattccat
     1861 catggtcttc aagatcggcg gctctatcat gtccgacacc gcagtgctgg aggacttcat
     1921 tcaggatgtg gtgctcctgc gctatatcgg catccatccc atcatcgttc atgggggcgg
     1981 cccggagatc tcccaggcca tggagaagtt cggcaagaag gcggagttcg taggcggcct
     2041 gcgggtgacc gatcgggaga ccttgagcat tgcccgcatg gtcttgcttg gtcacatcaa
     2101 tgcagagctt gtcaacctca tcggcaagca tgggggcaag ggcataggcc tatccggcca
     2161 ggacggcatg ttcctgaagg ccaagaagaa agctctgcag atggtgga
//
LOCUS       DAOSGP010000231         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_48933_length_2204_cov_18.236389, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000231 DAOSGP010000000
VERSION     DAOSGP010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48933_length_2204_cov_18.236389"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PLV49_06310"
     CDS             <1..1128
                     /locus_tag="PLV49_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HOG98493.1"
                     /translation="KRVLDSKLNRLGLQDIRVRMVDNKYILIDLAGMDVASAQDVVGK
                     PGKFEIRIQTEDNQTMHVIYGDAVESVDIPRGDQQGNWGVPFTLSEEGSAAFQKAAID
                     TGAVKNPEAHPITMYLDKDPIFSAPLSPELASSLQKVPSRGLEARVGAGDEGSQDAKE
                     LQIHLQEGALPVNVEIVGSGQVSAALGKQFKIQMVIAGLIALLAVAGMIYYRYRERRI
                     VLPMLFTSLSEVAIMLGVWSLAKWQLDLASLAGIIMVIGTGVDQLVIITDEVIRGGEA
                     APAASDRSIKERAAEAAEKAGVGKVTATTSKIYLSRLSRAFAIILGAAATTIVAMLPL
                     LYMGFGALTGFALIIIIGVVLGTLVARPAYGRIIGYILGTH"
     gene            complement(1205..>2204)
                     /locus_tag="PLV49_06315"
     CDS             complement(1205..>2204)
                     /locus_tag="PLV49_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HOG98494.1"
                     /translation="NRSTFQLMNERLKATLFAALMSLASIIFILYYTDADISWELISR
                     VNGWFLLLAVALHVLSWILYSLRLKLLASMAGHQITFPLSFRCTLASNFLAAITPSSA
                     GGEPLRIKVLADDGMSYGAATAVVISERLLDSIFFLTSLAFFLMVSGFFAGFGLKVGA
                     IFLLFLLAFLAFLRQLIIRPKRVARLMEWIKKKTGNRAIILTIEREIWLFRDAGIQLA
                     KETMRCLPVLVVMTALIWFSDFLVPSALLAGMAQDPSILLSVTAQNILAIVSLLPLTP
                     GASGIAELGMSYLYSTFVSPALLLPLIVLWRLITYFFNIVVGAAFAGAAINGMIKK"
BASE COUNT          563 a          562 c          653 g          426 t
ORIGIN      
        1 aaaagggttc ttgactccaa gctcaaccgc ctggggctgc aggacatcag agtgaggatg
       61 gtggacaaca agtacatcct catcgacctt gcgggaatgg atgttgcctc agctcaggat
      121 gtggtgggca agcctggcaa gttcgagata aggatccaga cagaggacaa ccagaccatg
      181 catgtcatct atggtgatgc agttgagtct gtggatatcc ccagaggaga tcagcagggc
      241 aattggggag tgcccttcac tctatccgag gaggggtcgg cagccttcca gaaggcagcc
      301 atagacacgg gcgcggtcaa gaatcccgaa gctcacccca taaccatgta cctggataag
      361 gatcccatct tcagcgcacc tttatcgcca gagctggcga gcagtctgca gaaggttcct
      421 tctcgcgggc tggaggcgcg cgtgggcgcc ggagatgagg gctcccagga tgcaaaggag
      481 cttcaaattc acctccagga gggcgctctg ccggtcaatg tggagatcgt gggatcagga
      541 caggtatctg ccgctctggg caagcagttc aagatccaga tggtgatcgc aggcctgatc
      601 gccctcctag cagttgcggg catgatatac taccgctacc gtgagaggag aatagttctg
      661 cccatgctct ttacctcctt gagcgaggtg gcgataatgc tgggagtctg gtccctggcc
      721 aaatggcagc tcgatctggc cagcctggcc ggcatcatca tggtgatagg gacgggcgtc
      781 gatcagctgg tcatcatcac cgatgaggtg atccgaggag gcgaagcagc gccagctgca
      841 tccgaccgga gcataaagga gagagcagcg gaggcggcag agaaggcagg cgtgggcaaa
      901 gtgactgcca ccaccagcaa gatctatttg agccggctgt cccgggcctt tgccataatc
      961 ctgggcgcag cagcgacgac gatcgtggcc atgctgccgc ttctctatat gggattcggt
     1021 gccttgaccg gattcgccct gatcatcatc attggagtgg tcctaggaac gctggtggcc
     1081 aggcctgcct atggcagaat cataggctac atcctgggca cacactagac ggaaaaagaa
     1141 gtggaatcag gagaggttat cggctgcagg ccggaagatg gtctgcatgc caattatttt
     1201 tgctctattt tttaatcatt ccattgatgg ccgctccggc gaaagctgca cctacgacga
     1261 tgttaaagaa ataggtgatc aaacgccaca ggacgatcag aggcagcagc aaggcgggag
     1321 agacaaaggt ggaataaagg tagctcattc ccagctcggc tatgccgctg gccccagggg
     1381 tcaacggcaa gaggctcact atcgccagga tgttttgggc ggtgactgag agaagaatgc
     1441 tgggatcctg agccataccc gccaataaag cagagggaac cagaaaatcg ctaaaccaga
     1501 ttaatgccgt catcaccacc agcaccggca aacaacgcat tgtctcttta gcaagctgaa
     1561 ttcctgcgtc ccggaagagc catatctccc gctctatggt gaggatgatg gctcggttac
     1621 cggtcttctt cttgatccac tccatcagac gggcaaccct cttcggccgg atgatgagct
     1681 gcctgagaaa tgccaggaaa gccaggagaa agagcaggaa tatcgctcca accttcaggc
     1741 cgaagccggc gaaaaagccg gagaccatga ggaaaaaggc cagtgaggtc aagaagaata
     1801 tgctatcaag gagcctctcg cttatcacca ccgccgtcgc agcaccatag ctcatgccgt
     1861 catctgccag gaccttgatt cgcaggggct ctcctccagc agaggatgga gtgatagcgg
     1921 caaggaagtt cgaggccagg gtgcatctga aggagagagg gaaggttatt tggtgaccgg
     1981 ccatggatgc aagcagcttg agcctgagag agtaaagtat ccaggagagc acatgaaggg
     2041 ctaccgccag aagaaggaac cacccgttga cccgggagat caactcccaa cttatatcag
     2101 catcggtata ataaagaatg aagataatgg aggccaggct catcagagcg gcgaacaatg
     2161 tggccttcaa tctctcattc atcaattgga aggtagatct attc
//
LOCUS       DAOSGP010000232         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_49090_length_2200_cov_14.260606, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000232 DAOSGP010000000
VERSION     DAOSGP010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49090_length_2200_cov_14.260606"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /gene="aroA"
                     /locus_tag="PLV49_06320"
     CDS             complement(<1..1253)
                     /gene="aroA"
                     /locus_tag="PLV49_06320"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOG98495.1"
                     /translation="MIASVECSAVSGEVYAPPSKSYTHRAILITSLGPGGRVLRPLLS
                     ADTLATVSASEAFGAKIGKEEKKDDLFIDGVGDLPQTPENVIDVLNSGTTLRFCSAVA
                     ALTEGAVLTGDSSIRTRPNGPLLDSLRELGADAFSIKNNGKAPLVIRGKMSGGTAHLQ
                     GGLSSQFLSALLIASPLAERETRIRIVGELKSRPYAEITLDMLHDAGIKIEEEEQEFI
                     VPGGQSFDLERYTIPGDFSSASYPLAAAAVTGSEVMVKGILPSRQGDSAIIDILTRMG
                     AQVSWNRESGDLRIKGGELEGIEIDAGRTPDLVPTIAVLGAVAKGTTTVVNAEHVRHK
                     ETDRLHAMAVELTKMGAKVKERPDGLEITGGKLHGASVHGYHDHRIVMALTVAGLVAG
                     ETRIDTAEAVDVSYPGFFREMERLGA"
     gene            complement(1250..1378)
                     /locus_tag="PLV49_06325"
     CDS             complement(1250..1378)
                     /locus_tag="PLV49_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98496.1"
                     /translation="MNAVITAEEVEEWGLLSLPFQVIVSGYIMALISYTSSFISRS"
     gene            complement(1455..1838)
                     /locus_tag="PLV49_06330"
     CDS             complement(1455..1838)
                     /locus_tag="PLV49_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOG98497.1"
                     /translation="MIVLDTSALIDLFRGGEEVKGVIGGDVTTTIINYYEIFVGIRRY
                     RSKTEEKFFRRFFSDIRILDFNLTAAERSSEIMARLMDLGISINAFDILISGIAVASG
                     GETLMTKDRDFLSIAKVTELNIKVY"
     gene            complement(1835..2053)
                     /locus_tag="PLV49_06335"
     CDS             complement(1835..2053)
                     /locus_tag="PLV49_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HOG98498.1"
                     /translation="MASKTISIRDDVYKLLKDAKREEESFSDAIERLLKKDKVNLSEY
                     FGALKEDPILDRLDADSKRIREMARSRV"
BASE COUNT          497 a          640 c          523 g          540 t
ORIGIN      
        1 gcccccagcc tctccatctc cctgaagaat cccggatagc tcacatccac tgcctcagcg
       61 gtatcgatcc tcgtctcacc ggccacaagg cccgccactg tcagcgccat gacgatcctg
      121 tggtcgtgat agccgtgaac gctcgccccg tgcagctttc cgcccgtgat ctccaggccg
      181 tccggccgct ctttgacctt cgcccccatc ttcgtcagct cgacggccat ggcatgaagc
      241 ctgtccgtct ccttgtggcg gacgtgctcc gcattcacaa ccgtcgtcgt gccctttgcc
      301 actgcaccca ggacggcgat ggtaggaacc agatccggcg ttctgccagc atcgatctct
      361 attccctcca gctctcctcc tttgatcctc agatcccctg attccctgtt ccaggaaacc
      421 tgggccccca tgcgggtcag gatatcgatg atggccgaat ccccctgtct ggagggcagg
      481 atccctttca ccatcacctc cgatcccgtg accgcagcgg cagccaatgg gtatgaagca
      541 gaggagaagt cgccgggaat ggtatacctc tccaggtcga agctctggcc tccaggtaca
      601 atgaactcct gctcctcttc ctctatcttt atccctgcat catggagcat atccagggtg
      661 atctctgcat aggggcggga cttcagctct cccacaatcc gaatcctcgt ctccctttcc
      721 gccaggggag aggcaattaa gagagcggag agaaactggc tgctcagccc gccttgcagg
      781 tgcgctgtgc cgccggacat cttccctctg atgaccaggg gggctttgcc gttattctta
      841 atggaaaagg catccgctcc cagctctctg agggagtcaa gcaacggtcc attgggccgg
      901 gtgcgaatgg aggagtcgcc tgtcagcact gccccctcgg tgagcgctgc caccgccgag
      961 cagaagcgca gcgtggtgcc tgagttcagc acgtctatca cattctctgg ggtctggggc
     1021 agatctccta ccccatctat aaagaggtcg tcctttttct cttctttccc tatcttggcc
     1081 ccgaaggcct cgctggcaga taccgtggcc agggtatcgg cagagagcaa tggccgcagg
     1141 actctccctc ccgggccaag agaggtgatc agaatagcgc ggtgggtgta gctcttcgag
     1201 ggaggggcat agacctcgcc ggatacagca gagcactcga ctgaggcgat catgaacggg
     1261 agatgaatga ggatgtataa gaaatgagtg ccatgatata gcctgacacg atgacctgaa
     1321 aaggaagaga gagaagcccc cactcctcca cctcctctgc cgttattacg gcattcaacg
     1381 tagtacgaca taagtcgaat ataagccttg cggcctgtac tggatcgtgc tcgaggacga
     1441 gtgagaatcg ttcttcagta aaccttaatg ttcagctcag tcactttggc aatggacagg
     1501 aaatctctat ccttcgtcat caaggtctca ccaccgcttg ccactgcaat gccggagatg
     1561 agtatatcga aggcattgat ggaaatcccc agatccatga gcctggccat gatctcgctc
     1621 gacctttccg cagcggttaa attaaaatcc aatattctaa tatcggaaaa gaatcttcga
     1681 aagaatttct cctcagtttt tgatctataa cgtcttatgc caacaaatat ttcataataa
     1741 tttattatag tagttgtgac atcaccacct atcacacctt taacctcctc gccacctcga
     1801 aacaggtcaa taagagcgct ggtatccagg acgatcaaac tctcgacctc gccatctctc
     1861 ttatcctctt agaatctgcg tccagccggt ctaaaatcgg atcttcctta agagctccaa
     1921 agtattcaga tagattgact ttatccttct tgagcagcct ttctattgca tcgctaaagc
     1981 tctcttcttc tctctttgca tctttaagca gtttatatac atcgtctcta atggaaatgg
     2041 tcttggatgc catgcatctg tgtatgtatg cacagatata tacggtttgg gattttgaac
     2101 tggaagcagc agaatggtgc ctcaaaggat aatttataaa gacatatctc aatggtgatt
     2161 cccggtatga aaatcatctg cgcctacccg gtaaatctcg
//
LOCUS       DAOSGP010000233         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_49516_length_2187_cov_17.144465, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000233 DAOSGP010000000
VERSION     DAOSGP010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49516_length_2187_cov_17.144465"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1293
                     /locus_tag="PLV49_06340"
     CDS             175..1293
                     /locus_tag="PLV49_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HOG98499.1"
                     /translation="MPRKRRELYNKEICACSIFGAMNKDGERFNAAGVMRAIANMHVR
                     GNGLGGGFAAYGIYPQYRDYYAFHLMFNGKTLQSQISAKAEFEQYLNQRFDVVYDEEI
                     PHRPQTQLRDPPLIWRYFASPRKRGEDSSLSDDEYVVRTVMHTNSRAEDAYIFSSGMN
                     MGCFKGVGYPEEIGEYFMLDQLYSAYAWTAHGRFPTNTQAWWGGAHPFSLLDTSVVHN
                     GEISSYGTNRWYLEMYGYACTLQTDTEVIAYAADLLLRRQRIPMEIVAKILAPPIWNA
                     IDTMNEKEKKLFTTLRMVYGPLLMNGPFAIIIGQTGRLIGLTDRIRLRPITAATKGEM
                     FYLSSEEASIRLISPELDSVWTPSGGEPVICELNSRRV"
     gene            1295..>2187
                     /locus_tag="PLV49_06345"
     CDS             1295..>2187
                     /locus_tag="PLV49_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOG98500.1"
                     /translation="MKSFVFPQFQIIRDEAKCGQCRGCERQCAFGTHIYDPVSDRLIS
                     DDNKCVGCQRCAIFCPRDAIVVRPNPSFYRPNASWSREKLEDVKKQAETGGVILTGCG
                     NDKPFRIYWDHMVLNASQVTNPSIDPLREPMELRTFLGAKPNLMDFKVKNEEVEIATQ
                     LSPNLMVETPIVFSAMSFGAISYNAFLSLATAASKLGTYFNTGEGGLPREMREKFGKH
                     AIVQVASGRFGIDAEYLNCAAAVEIKVGQGAKPGIGGHLPGEKVSVSVSATRMIPVGT
                     DAISPAPHHDIYSIEDLSMLI"
BASE COUNT          524 a          610 c          599 g          454 t
ORIGIN      
        1 ccaacaagaa ggcagagtgg gatgcctacc gcatgaacgt cacccagtgg gagcttaacc
       61 gctaccttcc actactgtga gcattctggg gacgaatctg gctgctcgat tcgccccagg
      121 gaattgtctc aaaagaaata gttgcaaggt cgctgtctgg agtttttggt tgctatgcca
      181 aggaagagaa gagagcttta caataaggag atctgcgctt gctccatctt cggggcaatg
      241 aacaaggatg gtgaacgctt taacgctgcc ggggtcatga gggccatagc caacatgcat
      301 gtgcggggca acggcctggg cggaggcttt gcagcctatg gcatatatcc gcagtatcga
      361 gactactatg ccttccatct catgttcaac ggcaaaaccc tccagtccca gatatcggcc
      421 aaagcggaat tcgagcaata ccttaaccag agattcgatg tggtctatga tgaggagatc
      481 cctcatcgcc ctcaaaccca gcttcgagat ccccctctga tatggcgcta tttcgcctcc
      541 cccagaaaaa ggggagagga ctccagcctg tcagacgacg agtacgtggt gagaacggtg
      601 atgcacacta acagcagagc ggaggacgcc tacatattct cctctggaat gaatatgggc
      661 tgcttcaagg gagtgggcta tcctgaggag ataggtgagt acttcatgct cgaccaactc
      721 tactctgcct atgcctggac ggctcatggc cggtttccca ccaacaccca ggcctggtgg
      781 gggggagctc atcctttcag cctgctggat acgagcgtgg tgcacaacgg cgagatctcc
      841 tcttacggca ccaatcgctg gtacctggag atgtacggct atgcctgcac ccttcagacc
      901 gataccgagg tcatagccta tgccgcagac ctactcctca gaaggcagag gataccgatg
      961 gagatcgtgg ccaagatcct ggccccgccc atctggaatg ccatcgatac catgaacgag
     1021 aaggagaaga agctcttcac cacactgaga atggtctatg gccccctgct tatgaacggc
     1081 ccctttgcca ttatcatcgg ccagacaggc aggctgattg gcctgacgga cagaatccgg
     1141 ctccggccga taacagcagc caccaaaggg gagatgttct atctctccag cgaagaagcg
     1201 tcgatcagac ttatctcgcc ggaattagac tccgtctgga cgccaagcgg aggagaaccg
     1261 gtaatatgcg aacttaacag caggagggta taagatgaaa tcattcgtct ttccccagtt
     1321 tcagatcatc agagacgaag caaaatgcgg ccagtgccgg ggttgcgaga gacagtgtgc
     1381 atttggcact catatctacg atccggtcag cgatcgattg atttcagacg ataacaagtg
     1441 cgttggttgc cagaggtgcg ccatcttctg cccccgggat gccatagtgg ttcgccccaa
     1501 tccgtcattc taccgcccga acgcctcctg gtctcgggag aagctggagg acgtaaaaaa
     1561 gcaggctgag actggcgggg tgatactgac cggatgcggc aacgacaagc ccttccggat
     1621 ttactgggac catatggtgc tcaatgcctc tcaggtcacc aacccctcga tagatcctct
     1681 gcgggagccg atggagctgc gcacctttct tggtgccaag ccgaacctga tggatttcaa
     1741 ggtgaagaac gaggaagtgg agattgccac ccagctctca cccaatctga tggtggaaac
     1801 gccgatcgtc ttctccgcca tgtccttcgg agcaatcagc tacaatgcct tcctctccct
     1861 ggccacagcg gccagcaaat tgggaaccta cttcaatacc ggcgaggggg ggcttcccag
     1921 ggagatgaga gagaaattcg gcaagcatgc catcgtccag gtagcctccg gtcgtttcgg
     1981 catagatgcc gaatacctca actgcgccgc tgcggtggag atcaaggtag gccagggggc
     2041 taaacctgga ataggcggtc acttgcccgg agagaaggtc tctgtttccg tctccgccac
     2101 ccggatgata cctgtgggca cagacgccat ctccccagcg ccgcatcatg atatctactc
     2161 catcgaggat ctgtccatgc tcatcca
//
LOCUS       DAOSGP010000234         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_49517_length_2187_cov_16.489681, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000234 DAOSGP010000000
VERSION     DAOSGP010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49517_length_2187_cov_16.489681"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..744
                     /locus_tag="PLV49_06350"
     CDS             382..744
                     /locus_tag="PLV49_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98501.1"
                     /translation="MSMNCCEHKISGSKEKIWLPHYFEGRDRGLKPHPYCTECGLVKS
                     LSSDRLHSVGYFMNVIAEIGKRHKITQVQTRLIALEMEKQLISDAFGIDRHQQELLFI
                     EITTRILNVSARSVTQLL"
     gene            complement(753..2024)
                     /locus_tag="PLV49_06355"
     CDS             complement(753..2024)
                     /locus_tag="PLV49_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOG98502.1"
                     /translation="MAWKPVMNSNLHRYLSCKAIAGMARDGLYRTSFFMAFANIMSAG
                     CGFFFWIVAARLYALEQVGLATALISSLALVALFSTLGFDSSIIRFLPQEDKGKVIST
                     SFIVTTGASIIFGGVCILLMMILTPSIPFWQDPGHSLAFILVAALNSIAVMGGYAFVA
                     DRKADHYFLQNLLQAIRIPALVPLAFLGVFGIFGAIGLGYLTAALYSLVKIQLSICRI
                     RPRADREFIRKSLRFSSLNYLSSLLYAAPTLMMPILVLSLLDEAEAAKYYIAFALGTL
                     VLIIPSSFGTSLFVEGSHGNSLKKSAFRAGGASLMIMLPAVLVLFFHGDRLLGLLKGE
                     YVEGFGLLKIIALSSFPVACYSLFIPIQNVRMRVESIVKLNALRCILLLGLSYVMVNR
                     YGILGAGYAWMITYMVIVIWVGWVALKEKWI"
BASE COUNT          626 a          492 c          530 g          539 t
ORIGIN      
        1 gaattaggct tccgagcttt ttgagatcaa tttttgcaga aaaggttcga gcccacatag
       61 tactgatttc aacaaattcg agtttttggc ttaattgcaa aatgtaaaat tgagtattaa
      121 tatagtacca gaaaatcctt aaccgatgcc gtatgggtgt gtattcgatc tgggattctt
      181 caaggttttg attccgacag attgtagtcc taatctaagg aatcgattat cgcgcaaaag
      241 gccatcaaag ctaaaagttt aataaaatat tattgaaaaa tttgtctctc atatacggca
      301 tcctttacca aaatactgca attgtctcgc agttccataa tttaaccaat ggttttttaa
      361 agctcttgaa ttcattaaag tatgagtatg aattgctgcg aacataaaat atctgggagc
      421 aaggaaaaaa tctggctgcc ccactatttt gaagggcggg atcgagggct caagccccat
      481 ccatattgta cggagtgcgg tcttgtcaag agcctctcct ctgataggct acacagcgtt
      541 ggctatttca tgaatgttat tgcagagatc ggcaagcgtc acaaaatcac tcaggtgcaa
      601 acgaggttaa tcgccctgga gatggagaag cagttgataa gcgatgcatt cgggatcgat
      661 cggcaccaac aggagctgtt gtttattgag ataaccaccc gtatcctcaa tgtctccgca
      721 aggtcggtaa ctcagcttct ttaaatataa tttcaaatcc acttttcctt taatgccacc
      781 catcccaccc aaataacgat caccatatag gttatcatcc aggcatatcc tgctcccagg
      841 atgccgtatc gatttaccat aacatagctc aatccaagta gcaggatgca cctgagggca
      901 ttcagcttca ctatgctctc aaccctcatc ctcacattct gaatggggat gaatagggaa
      961 taacaggcta cgggaaagct agagagggcg atgatcttca gaaggccaaa tccctccacg
     1021 tactctccct tgagaagtcc aagtagccgg tcgccatgga agaagagcac cagaacagcg
     1081 gggagcatta tcatcaggct tgcgcctccc gcgcgaaagg cgctcttctt gaggctattt
     1141 ccatggctcc cctccacaaa gagggaggtc ccaaaggagc tggggatgat aagcaccaga
     1201 gtgcccagag cgaaggcgat atagtatttt gcggcttcag cttcatcgag caggctcagc
     1261 accagtatgg gcatcatgag ggtaggtgca gcatatagaa ggctggagag ataatttaga
     1321 gaggagaatc gcaaggactt acggatgaac tctcggtctg cccgtggcct gatccggcat
     1381 atgctcagct gaatcttcac caagctgtag agcgctgcgg tcagataccc caggccaata
     1441 gctccgaaga ttccgaagac gcccaggaaa gcgagaggca caagggctgg aattcgaatt
     1501 gcctgcaaga gattctgcaa gaagtaatgg tccgcttttc tgtccgcaac gaaggcgtat
     1561 cctcccatga ccgcgattga gttcagtgct gctaccagaa tgaaggccag agaatgaccc
     1621 ggatcctgcc aaaatggtat ggaaggagtc agaatcatca tcaacaagat gcatactcca
     1681 ccgaagatga tgctggcccc ggtggtcaca ataaagctcg tgcttatgac cttcccctta
     1741 tcctcctggg gaaggaagcg gatgatcgag gagtcaaagc cgagggttga gaagagagcg
     1801 acgagtgcaa gagatgatat gagcgctgtg gccaggccta cctgctctag ggcataaagc
     1861 ctggcggcga caatccagaa aaagaagccg catcctgcgc tcatgatatt ggcgaaggcc
     1921 atgaagaagg atgttctata tagaccatct ctggccattc ctgcgattgc tttgcagctc
     1981 agataacggt gtagattgga gttcatgact ggtttccagg ccacagaagg catcaatggc
     2041 agatttatga attggatgcc actgatttgt ttctggtctt gtatctgatc ttggttgtat
     2101 aaatgttttc atgtaagagc ggtcgaaagc cgaagccata tatggtcaga cgatcattat
     2161 tacatatatc gaaaaatcaa aaagtta
//
LOCUS       DAOSGP010000235         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_49680_length_2182_cov_20.097320, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000235 DAOSGP010000000
VERSION     DAOSGP010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49680_length_2182_cov_20.097320"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1984
                     /gene="speA"
                     /locus_tag="PLV49_06360"
     CDS             26..1984
                     /gene="speA"
                     /locus_tag="PLV49_06360"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOG98503.1"
                     /translation="MWKAEDSIELYGIEKWGNGYFSVNEKGNVVVLPNKNLYQSVDIM
                     DLIEEIEKSRDLEFPVLLRFPQMLEDRIDEITGAFMGAIEEFNYSGTYQPIFPMKVNQ
                     RKEVIEYIIKYGAKHKIGMEVGTKAELLAALSLGLPRYAPLICNGYKDEDYLRLALSI
                     HNLNNIIIVVDLFEEIYDILKYADAMGVVPRVGMRVKLFARGSGRWVESGGESAKFGL
                     STSEALELMRILEERGLTSSLKMLHFHIGSQITDIRTVKNAMNEAARVYAKARKMADI
                     EYLNVGGGLSVDYNGSNTATPSSANYSLQEYANDIVYTVQKICDDEEVPCPTIVSESG
                     RAIAAYHSMLIFKIIGRKNAKSSPLRPPDDEAPMQIDDLCSAFKEINIDNYKEHYHDA
                     LQYRDELYDSFNLGNIGLEERAKGETLFWMVCKKAAFLAKEAGDESDEFLELKKLVSQ
                     KYIGNFSLFQSVPDMWGVEQIFPTIPLHRLDEMPSERGRIVDITCDSDGEIKRYAGDS
                     EGLEYLEMHPLLENQDYYLGIFLLGAYQDTLGDFHNLLGCAHEVHVMAEDGEWYICQK
                     VEGDTCKKLLDFFNYETKDYIWEIMDRCVTRKESIRKRELEQIEAELNRTLKGYTYFI
                     TRPPRQSKKKKKETERDLGQRRPEALKG"
BASE COUNT          554 a          553 c          610 g          465 t
ORIGIN      
        1 cagcagatga agaaggcgga gatctatgtg gaaagctgag gattctattg aactctacgg
       61 aatagaaaaa tggggcaacg gctatttttc tgttaacgag aagggaaatg tcgtcgttct
      121 tcccaataag aatttgtatc aatccgtgga tatcatggat ctcattgagg agatagagaa
      181 atccagggat ctggagttcc ccgtcctcct ccgctttccc cagatgctgg aggaccggat
      241 agatgagatc acaggcgcat ttatgggagc tatagaggag ttcaattatt ccggcactta
      301 tcagcccatc tttcccatga aggtcaacca gagaaaagag gtcatcgagt atatcatcaa
      361 gtacggagcc aagcataaga tcggtatgga ggtgggaacc aaggcagagc tactggccgc
      421 tctctctttg ggcctgccaa gatatgctcc cctgatctgc aatggctaca aggatgagga
      481 ttatctccgc ctcgccctga gcattcacaa tctgaacaat ataatcatag tggtagacct
      541 tttcgaggag atctacgaca tcctgaagta cgccgatgcc atgggcgtgg tgccaagagt
      601 cgggatgagg gtgaagctct ttgcccgcgg ctccggccgg tgggtggagt cgggtgggga
      661 gtctgccaag ttcggcctct ctaccagcga ggccctggaa ttgatgagaa tcctcgagga
      721 gagagggctt actagcagcc tgaagatgct ccacttccat atcggctcgc agatcaccga
      781 catccggacg gtcaagaacg caatgaacga ggctgcgagg gtttatgcca aagcgcgaaa
      841 gatggccgat atcgagtatc tgaatgtggg cggcggcctc tctgtggact acaacggctc
      901 gaacaccgct accccttcca gcgccaacta ttctctgcag gagtacgcga acgacatcgt
      961 ctatacggtg cagaagatct gtgatgatga ggaggttccc tgtcccacca tagtctccga
     1021 gagtggaagg gccatcgccg cctaccacag catgctcatc ttcaagataa tcggcagaaa
     1081 gaacgccaaa agctcccctc tccggccccc ggatgatgag gcacccatgc agatcgatga
     1141 cctctgcagc gccttcaaag agatcaacat cgataactac aaggagcact accatgatgc
     1201 tctgcagtat agagacgagc tttacgactc cttcaatctg gggaacatcg gccttgagga
     1261 gagggccaag ggcgagaccc tattctggat ggtctgcaaa aaggcggcat tcctggccaa
     1321 ggaggcaggg gacgagtccg atgagttttt agagctgaaa aagctggtca gccagaagta
     1381 tatcggcaac ttctctctct tccagtccgt tccagacatg tggggggtgg aacagatatt
     1441 cccaaccatt cctctgcacc gcctggatga gatgcccagc gaacggggca ggatcgtgga
     1501 tatcacctgc gattcagacg gggagatcaa gaggtatgct ggagacagcg agggcctgga
     1561 gtacctggag atgcatcccc tgctggagaa ccaggactat tatctgggca tcttcctcct
     1621 gggagcatat caggataccc tgggagattt tcataacctt ctgggctgcg ctcatgaggt
     1681 gcatgtcatg gcagaggatg gcgagtggta tatctgccag aaggtggagg gcgacacctg
     1741 caaaaaattg ctggatttct tcaactacga gaccaaggac tacatctggg agatcatgga
     1801 ccgctgcgtg accagaaaag agagcatccg caagcgagag ctggagcaga tcgaagcaga
     1861 gctgaaccgc accctcaagg gttataccta cttcatcacc cgtcccccgc ggcagtcgaa
     1921 gaagaagaag aaagagacgg aaagggatct gggtcagcgg cgtcctgaag cactgaaagg
     1981 ctgaaaagca attgccgcat cccaggtggc aataatctta agttcctgac tgcattacca
     2041 gactgctgat gcattccacg tcccaggtcc gcagccctct actggccatt cctggagttc
     2101 tggccagcat tttttctctg gccataggtc tggtggctct cttgacctcc cttgcctcct
     2161 ttggccgcca ggcagagtat gg
//
LOCUS       DAOSGP010000236         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_49904_length_2175_cov_13.764623, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000236 DAOSGP010000000
VERSION     DAOSGP010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49904_length_2175_cov_13.764623"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLV49_06365"
     CDS             <1..401
                     /locus_tag="PLV49_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98504.1"
                     /translation="CSRCDFFVGREMSISAVIVRAEWGRRSSDQIFSRFFVALREGDD
                     LVPVGWIGSQLGQKDILELDRQLKALAREWDDTGACVNPQVLLNLKIQGARRNDKGYA
                     IVRPQVLGIDLFASWEDADELNSLFSSSGR"
     gene            398..1252
                     /locus_tag="PLV49_06370"
     CDS             398..1252
                     /locus_tag="PLV49_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98505.1"
                     /translation="MILRAFDPWKSELCTCPAKLSLNPYTGCPHGCLYCYASSYIPRF
                     YSCRPKVDLLRRLDREAAKVVPGLFIAISNSSDPYPPIEKELGLTRGCLRILKERNFR
                     VQIVTKSDLVTRDIDLLVAMKAAVSVTVTTLDESLSRRLEPGAPSPKRRLFALNELSR
                     AGIPLSARIDPIIPGINDCGIEDLVCSLHSAGVRHITSSTYKARPDSIKKITAAFPEE
                     GAALKELFSRGGRNSGSSYLPVDLRRSILEKVKSHAHQAGITFSTCREGFVFEEGISC
                     DGSHLLPL"
     gene            1481..>2175
                     /locus_tag="PLV49_06375"
     CDS             1481..>2175
                     /locus_tag="PLV49_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98506.1"
                     /translation="MKNVLAISMAIMIAAIVMMPALGYSEQMAENPSYTVASAGNQSY
                     SAGSAGSQSYTATSAGVQSNTAANAGRKGYSISTGVAAHNLTSDMVTEVNSVSASPEL
                     GIRIPYSIKDGSMVPYSIQQGFIVPYSPKLGSIVPYSIQMGSYASYSIKLMDETNATL
                     RGTYQTVKLEPKKLGSIARKETVALAGETPSVAAVETPEVQEVALNETAALNETVALN
                     ETVALNETVALNET"
BASE COUNT          565 a          539 c          561 g          510 t
ORIGIN      
        1 aatgcagcag atgcgatttc tttgtgggaa gagagatgtc catatcagcc gttatagtcc
       61 gggcggagtg gggccgaagg agcagtgatc agatcttctc tcgatttttc gtcgctctgc
      121 gagaagggga cgacctcgta ccagtgggct ggatagggag tcaattgggc cagaaggata
      181 ttctggagct ggatcgccag ttgaaagctc tggccagaga atgggatgat actggtgctt
      241 gtgtcaatcc ccaggttctc ctcaacctga agatccaggg tgctcgcagg aacgataagg
      301 gctatgccat cgtccgccct caggttttag ggatcgatct ttttgcctcc tgggaggatg
      361 cggatgaact gaatagtctt ttttcatcct ctggccgatg atcctcagag catttgaccc
      421 ctggaagagc gagctgtgca cctgtcctgc caagctcagc ctcaatccct ataccggttg
      481 cccacacggc tgcctttact gctatgcctc atcttatatc ccccggttct attcctgccg
      541 gccgaaggtc gatctattga ggaggcttga ccgagaggcg gccaaggttg tccccggcct
      601 atttattgct atctccaaca gctctgatcc ttatcctccc atagaaaagg agttgggatt
      661 gaccagagga tgcttgagga tactgaaaga gaggaacttt cgggtgcaga tcgtcacgaa
      721 gtccgatctg gtcaccaggg atatcgatct tctcgtcgca atgaaggcgg cggtgtccgt
      781 cacagtcacc acattggatg agtctctaag ccggaggctg gagccggggg cgccatcgcc
      841 aaagagaagg ctctttgcac tgaatgagct ctctcgggca ggaatacccc tgtcagccag
      901 aatcgatccg atcatcccgg gaatcaacga ctgcgggatc gaggacctgg tctgttccct
      961 tcattccgct ggggtcaggc atataacctc ctccacatat aaggcccggc cagatagcat
     1021 aaaaaaaatc accgctgcct ttccggagga gggagcggcc ttaaaagagc tcttctcaag
     1081 aggagggaga aattcgggca gcagctatct tccggtggat ttgagaagga gcattctgga
     1141 gaaagtaaaa tcccatgccc atcaggctgg catcaccttc tccacatgcc gggagggatt
     1201 cgtatttgag gagggcataa gttgcgatgg ctctcatctc ctgcccctgt aatcagcagc
     1261 taaattagcg aagcgtataa aaaataaatt aaatagtctg tataaaatat agatatcgaa
     1321 aaaaatcata aatgcaatgc gtcaacctta tggtgccctt tgcccacgaa agtttattta
     1381 tatgaagaat aactagaatc cacgacaatt cttctcaaaa gggaggatgt tcaagccata
     1441 gtcataaatg tcatatgcaa gagcaaaagg aggattcgat atgaaaaacg ttctggccat
     1501 cagcatggcc atcatgatag cagccattgt tatgatgcct gctctgggtt attctgaaca
     1561 gatggcagaa aatccgtctt atacagtggc ctcggcaggg aatcagtctt attctgcagg
     1621 ctctgcagga agtcaatctt ataccgctac atcagcagga gtgcagtcca ataccgcagc
     1681 caatgctgga agaaaaggct atagcatctc aacaggagtt gccgcccata acctcacctc
     1741 ggatatggta acagaagtaa attctgtgag cgcctctcca gaactgggta tccggattcc
     1801 ctactccatc aaagacggat ccatggttcc ttactccatc cagcaaggat tcattgtccc
     1861 atactcgcca aagctgggat ccatcgttcc ctactcgatc cagatgggat catatgcttc
     1921 gtactctatc aagctgatgg atgagaccaa cgccaccctg agaggcacct atcagaccgt
     1981 gaagcttgag ccaaagaagc tgggcagcat tgcccgcaaa gagaccgtag cattggcagg
     2041 cgaaaccccg tctgttgccg cggtcgagac accagaggtc caggaagtag ctctgaatga
     2101 gactgcagct ctgaatgaga ctgtagctct gaatgagact gtagctctga atgagactgt
     2161 agctctgaat gagac
//
LOCUS       DAOSGP010000237         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_50962_length_2145_cov_22.681818, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000237 DAOSGP010000000
VERSION     DAOSGP010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50962_length_2145_cov_22.681818"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..>2145
                     /locus_tag="PLV49_06380"
     CDS             278..>2145
                     /locus_tag="PLV49_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG98507.1"
                     /translation="MTSDQCKKSIISFNMALMLAFIFALCSISFSILLKDELQIAASD
                     VLAILVDSLAALALFYAARRSSAYGRQVRMAWTIFAFALVAHTLGDIAWSYTEIVLDQ
                     PPFPSMADWLFLIQYPLFIAGILLLPTQSLTSSERLKVMLDEGIVIIASVIIFWVVLI
                     APTIESSAGADIRTQAFSVVYPVMGLMLLFALVELLFRRIRFQQMGPVLLLVIGTAVM
                     IITDLILVYQSLNNRYDSGGIWDVGWIMAYSMIGLAGILQASISPGEIPPFLKAPFDE
                     PAYARTGASSASPYALDSGMVQFPGARYIPYIFAAGAYIILLWSFNHPLAISTPHLSY
                     SVGGIIGLIVIRQIVALKENERFFQTSKRAEEEVRRLNQHLESRVMDRTAMLKAANRK
                     LEEEILDRKKIEEDLRRSKEAAEAATNAKSEFLANMSHEIRTPLNAVIGLTGILLGTD
                     LNREQCDHLEIIRSSGDALLAIINDILDFSKIDSGKMELEVQPFDIKKSIEDAVDLMA
                     PVAQEKGLTLGYDIEESIPKTIVNDPTRLRQILVNILGNAVKFTSSGEVRILVTGRDL
                     GGREHEIHFAVMDTGIGIPAEKMGCLFQSFCQVEPCTTRRYGGAGLGLAISKRLVEL"
BASE COUNT          529 a          512 c          552 g          552 t
ORIGIN      
        1 ttaatccccc ttatgctcaa tccataattc atatcattat agttaaattt ttctattatt
       61 tccgcataag tcctcaggga ctgatcgggg caatgatgca aagctttttc tatcagtatt
      121 agagaacctg atctgcattc agagactata atatggcgaa aaaagctgca aaaaaggaag
      181 gctaataatt gatacatctt catcaggggt tccgaagaca gttatgatag gccaataata
      241 gatatgcaca gataagcagc aagatgaagg tttgcccatg acctctgatc aatgcaaaaa
      301 gtcgatcata tcgtttaaca tggccttgat gctggccttc atctttgccc tctgctcaat
      361 ctcatttagc atcctcttaa aagatgagct gcagatcgcg gcgagcgatg tgctggcgat
      421 cctggtcgat tctctggccg cactagccct tttttatgct gccaggaggt cttctgccta
      481 tgggagacag gtccgtatgg catggacgat tttcgctttt gccctggtgg cccatacctt
      541 gggtgacatc gcctggtctt atactgagat agtgctggat cagccaccct tcccatccat
      601 ggcagactgg ctgtttttga ttcagtaccc cctcttcatc gcgggcattc tcctcctgcc
      661 cactcaatcc ctgacctcca gtgaacggct gaaggtcatg ctggatgaag ggatcgtgat
      721 catcgcctca gttataatat tctgggtggt tttaatagca cccacgatcg agtccagcgc
      781 aggagcagat atccgcaccc aggcgttttc tgtggtatac ccagtcatgg gtttgatgct
      841 tctctttgcg ctggttgaac tcctctttcg gcggatccga tttcagcaga tgggtccagt
      901 gcttcttctg gtgattggaa cggcagtgat gatcatcact gatctcatcc tggtatacca
      961 atcattgaac aacagatatg attcgggcgg catttgggac gtgggctgga taatggccta
     1021 ctcgatgatc ggcctggcgg gaatactgca ggccagcata tcaccgggcg agataccacc
     1081 attcttgaag gctccattcg atgagcctgc ttatgctcgc accggtgcat cctctgcatc
     1141 tccttatgct cttgattccg gcatggtgca gtttcctggt gctcgctaca tcccttacat
     1201 cttcgctgct ggcgcataca tcatccttct ctggagcttt aaccatcctc ttgccataag
     1261 cacgcctcat ctatcatatt ctgttggagg gataataggt ctgatagtca ttcgccagat
     1321 cgtggccctg aaggagaatg agagattttt ccagacatcc aaaagagcag aggaggaggt
     1381 gcgaaggctt aaccagcatc tagagagcag ggttatggat aggacggcta tgctgaaggc
     1441 ggcaaaccga aagctagagg aggagatttt agatcgaaag aagatcgaag aggatctgag
     1501 aagatccaag gaggcggcag aggcggcaac caacgcaaaa tccgagtttt tagccaatat
     1561 gagccatgag atcagaaccc ccctgaatgc cgtcatcggc ctcactggaa ttctcctggg
     1621 cacggatctg aatagagagc aatgcgacca tcttgagatc atcagaagca gtggagatgc
     1681 acttttagct atcatcaatg atatcctgga cttctcgaag atcgatagcg gcaagatgga
     1741 gctggaggtc cagccttttg atatcaaaaa gagcattgag gatgcagttg atctgatggc
     1801 tccagttgcc caggaaaagg gcctgacctt gggatatgat atcgaggaga gtattccaaa
     1861 gaccattgtc aacgatccca ccaggctgcg ccagatactg gtcaacatat tgggcaatgc
     1921 ggtgaagttc accagctctg gagaggtcag gatcctggtt acgggcagag atctgggggg
     1981 tagagagcat gagatccact tcgcggttat ggatactggc atcggaatac ctgcagagaa
     2041 gatgggctgc ctctttcagt ccttctgcca ggttgagccc tgtaccactc gcaggtatgg
     2101 tggcgcaggc ctgggcctgg caataagcaa gaggctcgtg gagct
//
LOCUS       DAOSGP010000238         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_52005_length_2116_cov_15.110626, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000238 DAOSGP010000000
VERSION     DAOSGP010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52005_length_2116_cov_15.110626"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLV49_06385"
     CDS             <1..694
                     /locus_tag="PLV49_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOG98508.1"
                     /translation="ELIIKRRSGSSDPYRELKKESNRVARDLLPRAMEFYEQSQDKIE
                     ALIRIASAANSMEFGVKGHDFDNLTFGRVFADTLQENLHADMAELKRRLQSFDKILYL
                     TDNCGEVIFDLFVIEKLKEMGKTVVVGSKSEPIINDVTADELKSFSDVDVIPTGNVVG
                     TALEHLSPEASVLLFDPEWLILSKGMGNFETMSEFDQRLKGRLIYILRAKCEPVAAKM
                     AVPRGSLVARLI"
     gene            complement(691..1353)
                     /locus_tag="PLV49_06390"
     CDS             complement(691..1353)
                     /locus_tag="PLV49_06390"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOG98509.1"
                     /translation="MFESRVVCICGLPGIGSVGKVAADYLSNALKCSSIKPFYSHSFP
                     SQVMVSDGLAELMHAELLRSQDKENIFVLSGDTQPLDVQGMYQLAGEILQAIESEGVT
                     DVITLAAFVGDATAKILGSATDPESAAILHDSGIPLLRSGAIGGMNGLLAGLAPLYNM
                     RGYCLLGTSSGADLIDIAAATNLLYAIRDLFKLDLDFSMMESILDEPDEPVPEEVDMN
                     YC"
     gene            complement(1362..1961)
                     /locus_tag="PLV49_06395"
     CDS             complement(1362..1961)
                     /locus_tag="PLV49_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98510.1"
                     /translation="MIFLIYGLCFVASCFGAMAADTSSESGYTTLAQDSGKNASDMNI
                     SLFTITDEFDIEGLDVAEAVKFKAPSSDWKLYGVKVLGWSDYNSTDYSYNMDRNFLLE
                     IRDKDLHLLYRFADAQNGYFLTDQGPIWGVIEIPPLAVTEDFYVLFYDRGGMVVGMEY
                     NNGTGNSFLYGNGILTPAQWTTEANETIGINWMIEAVGK"
BASE COUNT          526 a          543 c          536 g          511 t
ORIGIN      
        1 agagctcatc attaaaaggc gaagcggaag ctcagatcca tatcgggaac tgaagaagga
       61 gagcaatcgg gtggcaaggg acctcttgcc gcgggcaatg gagttctatg aacagtccca
      121 ggacaagatc gaggccttga tcagaattgc ctcggcggcc aactccatgg agtttggggt
      181 caagggccac gattttgata acctgacatt tggcagggtc tttgctgata cccttcaaga
      241 gaacctgcat gcggatatgg ctgaattgaa aagacggctc cagtcttttg acaagatact
      301 ttatctgaca gacaattgcg gcgaggtcat attcgatcta ttcgtaatcg agaagctaaa
      361 ggagatgggc aagaccgtgg tggtgggatc gaagtccgag cccatcatca atgatgtcac
      421 cgccgatgaa ttgaagagct tctcggatgt ggatgtgata ccaaccggca atgtggtggg
      481 aacggcacta gagcacctga gcccagaggc ctcggtcctc ctatttgatc cggaatggct
      541 gatactgtcc aagggcatgg gcaacttcga gaccatgtcc gagttcgatc agaggttgaa
      601 gggcaggctg atctacatcc tgcgtgccaa gtgcgagcct gtggcggcta aaatggcagt
      661 tccaagggga tcgctggtag cgaggctaat ttagcaatag ttcatgtcca cttcttccgg
      721 gacgggctca tccggctcat cgaggatcga ctccatcatt gagaaatcca gatcgagttt
      781 gaacaggtct ctgatggcat aaagaaggtt tgtggccgcg gcgatatcta tcagatcggc
      841 tccggagctt gttcccagca ggcaatagcc tctcatatta tagagaggcg cgagccctgc
      901 caaaaggccg ttcataccgc cgatagctcc gctgcgtagc aggggaatcc cagaatcatg
      961 gagaatagct gccgattcag gatctgttgc cgagccgagt atcttcgcag tcgcatctcc
     1021 cacaaatgcg gccagggtta ttacatccgt taccccttca gattctattg cctgcaaaat
     1081 ctctcctgcc agctgataca tcccctgcac atccaggggc tgggtatccc cggagagcac
     1141 gaatatgttc tccttgtctt gggaacgcaa tagctctgcg tgcatgagtt ccgccaggcc
     1201 atcagacacc ataacctgag acgggaagct gtgggaatag aacggtttaa tgctactgca
     1261 tttcagagca ttgcttaggt agtcggcagc cacttttccc acgcttccaa ttcctggaag
     1321 gccgcagatg cagacaactc gagactcaaa catcgtctat atcacttgcc cacagcctct
     1381 atcatccagt tgattccgat cgtctcattg gcctcagtgg tccattgtgc cggcgtgaga
     1441 attccattgc cgtacaggaa ggagttgcca gttccgttgt tatattccat gccaaccacc
     1501 attcctccgc gatcataaaa taacacatag aagtcttcgg tgaccgccaa aggtgggatc
     1561 tctatgactc cccagattgg gccttgatcg gtgaggaagt agccattctg ggcatcagca
     1621 aatctgtaca ggagatgaag gtccttatct ctgatctcca ggaggaagtt cctatccatg
     1681 ttgtagctgt aatcggtgct gttatagtcg ctccagccca ggaccttcac accataaagc
     1741 ttccagtctg aactgggagc cttgaacttt acggcttctg ctacatccag accttcgata
     1801 tcaaactcat cggttatggt gaacaggctg atattcatat cggaggcatt cttgccgcta
     1861 tcctgcgcaa gagtggtata gccggattcc gatgacgtat ctgcagccat ggctccgaag
     1921 cacgatgcca caaagcatag cccgtatatc aaaaatatca tcttattcaa atgatcaccc
     1981 cgtatattct gatgaatccg gcccatataa ggattttgaa gagtcttatt gcagtctggc
     2041 agatcatcta ccagaccgca attcgtctgc aggactcagc ctgctctttc actcgccaac
     2101 tgctctgatc agccaa
//
LOCUS       DAOSGP010000239         2115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_52040_length_2115_cov_18.044660, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000239 DAOSGP010000000
VERSION     DAOSGP010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52040_length_2115_cov_18.044660"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /gene="truD"
                     /locus_tag="PLV49_06400"
     CDS             complement(<1..797)
                     /gene="truD"
                     /locus_tag="PLV49_06400"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOG98511.1"
                     /translation="MKLASEQDRFLGMDYFITDSPGCGGIIRRNPEDFLVEEVFEDLR
                     YEGGRYLVIEVEKTDWDTHHLIREMSRHLRISQKRFGWAGTKDKRAVTRQRISIMNLD
                     ESELDRIRLPDIKINVLGRTNRAVGLGDLLGNRFSITIRELSCPDPARSLASVSEEIK
                     RHKGVANYFGVQRFGDIRPITHLVGEALARGKAEEAARIFLALPYAGEQERTREARER
                     LWESGDIQAARNDFPGYLHHELAMLNYLAEHPGDYAHSFDVLSVNLKR"
     gene            complement(804..1157)
                     /gene="pth2"
                     /locus_tag="PLV49_06405"
     CDS             complement(804..1157)
                     /gene="pth2"
                     /locus_tag="PLV49_06405"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HOG98512.1"
                     /translation="MEFKQCIVIREDLKLSAGKLAVQVAHAAVMAVERAEKMDRSVVS
                     EWKAEGQKKVVLRVSGVSDLFRLREDAERAGIPSAIVADAGLTEIPPGTITALGLGPA
                     HGKLMDKVTGKLRLV"
     gene            complement(1216..>2115)
                     /gene="dnaG"
                     /locus_tag="PLV49_06410"
     CDS             complement(1216..>2115)
                     /gene="dnaG"
                     /locus_tag="PLV49_06410"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HOG98513.1"
                     /translation="EEIKQSVRVEEIIHLGKDNLPAGPNVLDSDAILVVEGRADVLNL
                     LKYGIKNAVAVGGTNVPPTIADLCAKKVVTAFTDGDRGGELIVKELLQVADIDYVARA
                     PDGKSVEDMTQKEIVRALRQKIPVEQVLDQYKIKNQPRKRREITTKRKSLIRERPLCV
                     RNAAERAEAIPPRRIVPREVVPKSAPVVEEIVVAAPQEPAPKPEPEREWFRQHMDELD
                     GTLCARLMDKNHNVLREVAVRDLARELKDSNGDVNGVIFDGVVTQRVLDIAADKGLEY
                     LIGAKMGNIVKSPSCVRVVTKEA"
BASE COUNT          397 a          683 c          525 g          510 t
ORIGIN      
        1 cgcttcaaat tgacggaaag aacatcaaag gaatgggcat agtcaccagg atgctcggcc
       61 agatagttga gcatggccag ctcatggtgc aagtagccag gaaaatcatt gcgggcggcc
      121 tgaatatccc cgctctccca cagcctttct ctcgcctctc tcgtcctctc ctgctctcca
      181 gcatagggta gggcgagaaa tatgcgggca gcctcttcgg cctttcccct ggccagggct
      241 tcgcccacca gatgggttat cggccttatg tctccaaacc tctgcacacc gaagtagttt
      301 gcaaccccct tgtgcctctt gatctcctcg ctgacggagg cgaggctacg ggccggatcg
      361 gggcaggaga gctctcgaat ggtgatggag aagcggttgc ccaaaaggtc gcccagacct
      421 actgccctgt tcgtcctgcc cagcacattt atcttgatgt cggggaggcg gatcctatcg
      481 agctccgatt catccagatt catgatgctg atcctctgcc tggttactgc cctcttgtcc
      541 tttgtccctg cccagccgaa cctcttctgg ctgatacgca aatgcctgga catctcccgg
      601 atgaggtgat gggtatccca gtcagtcttc tctacctcta tgaccaggta ccttccccct
      661 tcatagcgga ggtcctcgaa gacctcctcc accaggaagt cctcgggatt tcttctgatg
      721 atgcctccac agccaggact gtcggtgatg aagtagtcca tgcccagaaa tctgtcctgc
      781 tcgcttgcca gtttcatcgt ccctcagacc agcctgagct tgcccgtaac cttatccatc
      841 aattttccgt gggccgggcc caggcccagg gcggttatgg tgccgggagg gatctcagtc
      901 aggccagcat cagctacaat ggccgagggt attcccgccc tctctgcatc ctcacgcagg
      961 cggaagaggt ccgagacccc cgatacccgc aagaccacct tcttctggcc ctctgccttc
     1021 cattcggaga caactgaccg gtccatcttc tccgccctct ccaccgccat cactgctgca
     1081 tgagccacct gcaccgccag ctttcccgcc gatagcttca gatcctctct gataacaatg
     1141 cactgcttga attccatgaa aacccgcctc gttactgata aaaaaagttc ttgaaaaata
     1201 aaaagaggcg aaatgctatg cctctttagt gaccactctt acgcatgagg ggcttttcac
     1261 tatattgccc atcttggcgc cgatcagata ctccaggccc ttatcggcag cgatatccag
     1321 gacgcgttgg gttactacgc catcgaatat cacaccgttg acatctccat tggagtcctt
     1381 cagctctctg gccaggtccc tcactgccac ctcccggagg acgttatggt tcttgtccat
     1441 gagtctcgcg caaagtgtcc cgtccagctc atccatatgc tgccggaacc attcccgttc
     1501 cggctcgggc ttgggagcag gctcctgcgg ggcggcgacc actatctcct ccaccacggg
     1561 agccgatttg ggcactacct ccctggggac gattctgcgg gggggtatgg cctctgccct
     1621 ctccgcggca tttctcacgc atagcggcct ctcccggatt aggctcttcc tcttggtggt
     1681 gatctccctt ctcttccggg gctggttctt gatcttgtac tgatcgagca cctgctccac
     1741 cgggatcttc tgtcgcagag cgcggacgat ctccttctgg gtcatatcct cgacgctctt
     1801 cccatccggt gccctggcca cataatcgat atctgctacc tggagaagct cttttactat
     1861 cagctcgccg ccccggtcgc catcggtgaa ggcggtcacc accttcttag cgcagaggtc
     1921 ggcaatggtg ggaggaacat tggttccgcc cacagctacg gcgttcttga tgccgtactt
     1981 gagcagattg agcacatccg ccctgccctc aaccactaaa attgcgtccg agtcgaggac
     2041 attgggtccg gcggggaggt tgtccttgcc caaatggatt atctcctcca cccggacgga
     2101 ctgctttatc tcctc
//
LOCUS       DAOSGP010000240         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_52343_length_2107_cov_14.823587, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000240 DAOSGP010000000
VERSION     DAOSGP010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52343_length_2107_cov_14.823587"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLV49_06415"
     CDS             complement(<1..380)
                     /locus_tag="PLV49_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOG98514.1"
                     /translation="MKIKSRHHLKGSDARKVVACIEPFLGDSSVLRKASLERAVSDEG
                     LDLIFVNGRPLMMIVDDEPFFTVLGAIDLEPSKRLVVVDSGAVRFVVNGADIMKPGIV
                     SADPEIAPGDLVVIVEERHKKPLA"
     gene            472..690
                     /locus_tag="PLV49_06420"
     CDS             472..690
                     /locus_tag="PLV49_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695429.1"
                     /note="Enables 3` processing of polyadenylated mRNAs and
                     tRNA precursors; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small nuclear ribonucleoprotein"
                     /protein_id="HOG98515.1"
                     /translation="MGQRPLDILNESLNGPVIVKLKDGRVFRGELQGYDIHMNLVMDK
                     TEEVAEGAVARNIGTVIVRGDNVVYISP"
     gene            701..871
                     /locus_tag="PLV49_06425"
     CDS             701..871
                     /locus_tag="PLV49_06425"
                     /inference="COORDINATES: protein motif:HMM:NF003214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HOG98516.1"
                     /translation="MTKGTPSMGKRHKSSHIRCRRCGSISFNFKRKVCVQCGFGNSAK
                     LRSHKWMRKADY"
     gene            885..>2107
                     /gene="purF"
                     /locus_tag="PLV49_06430"
     CDS             885..>2107
                     /gene="purF"
                     /locus_tag="PLV49_06430"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG98517.1"
                     /translation="MHDACGVVGISLNETANGAAKSIYYSLYALQHRGQEAAGISVHD
                     GKSIRTHRAMGLVSEVFDDAQIALLRGHVGIGHVRYATSGRSCIENSQPLLVKYKDGA
                     IATAHNGNLVNSTQLREQLEEAGDIFYSTSDTEVIAHIFVKELLHYDLLEAARSLMRR
                     IVGSYSLVFLWGDTVLALRDPLGIKPLCIGEIDSGFMVASESVAIDTLNGKLIRDVKP
                     GELIVIRNGEMKSYQLARSTRPAHCIFEYIYFARPDSIMDGRLIYDVRVNIGSNLARE
                     HPACADTVTPIPDSGITLAVGYHQHSGISYRECLMKNRYIGRTFIMPDQNMRETAVRL
                     KMNTIRPNIEGHKLILVDDSIVRGTTSRRIVNMVRKAGAQEVHVRIGSPPILAPCYLG
                     IDMASRDELIAAHKTV"
BASE COUNT          514 a          564 c          566 g          463 t
ORIGIN      
        1 atggccagag gcttcttatg cctctcctct actatcacca ccaggtctcc gggggcgatc
       61 tccggatcgg ctgagactat ccccggtttc atgatgtccg ccccgttcac cacgaaccgc
      121 acagcacctg aatccaccac caccagtctc ttgctgggct ccagatcaat agcccctaaa
      181 acggtgaaga agggctcatc atccacaatc atcattaaag gcctgccgtt cacgaagata
      241 agatcaagac cctcgtcgct gaccgccctc tccagggatg ctttgcgcag gacagatgag
      301 tcacccagga atggctcaat gcatgctaca accttcctgg catcgcttcc tttgaggtgg
      361 tgcctggatt taatcttcat atgctgtgga tatcccgata ggtttaaata tgcaacgagc
      421 aagtttggaa atcgtagcct tttgcggtta tcaatgctgg agagaggatt aatgggtcag
      481 agaccgcttg atattctgaa tgaatcactg aatgggcccg tcatagttaa gctcaaggac
      541 ggaagggtat tccggggaga gcttcagggt tatgatattc acatgaacct ggtgatggac
      601 aaaacggaag aagtagcaga aggggcggtt gcccgtaaca tcggtacggt aatcgtgcgc
      661 ggagacaatg tagtttatat ttcaccttga ggtgttttag atgaccaagg gtactccttc
      721 aatgggtaag cgccacaaga gctcgcatat aagatgccgg cgatgtggta gcatctcgtt
      781 taatttcaag agaaaggttt gcgttcaatg cggctttggc aactcagcaa agctcagaag
      841 ccataagtgg atgagaaaag cggactacta gagagaggcg aaatttgcac gatgcctgcg
      901 gcgttgtagg gatctcatta aatgagactg ccaatggcgc agctaaatcc atctattact
      961 ccctctatgc tctgcagcat agggggcaag aggctgctgg catctcagtg catgatggca
     1021 aatccattcg cacccaccgg gccatgggct tggtctcgga ggtcttcgac gatgcccaga
     1081 tcgcccttct gcgcggacat gttggcatcg gccacgtccg atatgcgacc agcggccgat
     1141 cttgcataga gaacagccag cctcttttag taaaatataa ggacggtgcc attgccaccg
     1201 cccataatgg taacctggtc aactccacac agctcagaga gcagctggag gaggcagggg
     1261 acatattcta ctccacctca gacaccgaag tgatcgctca catttttgtc aaagagctcc
     1321 ttcactatga cctgctggaa gcagcccgct ccctgatgag aaggattgtg ggctcttact
     1381 ccctggtatt tctctggggg gacactgtcc ttgcgctacg cgatccgctg ggaatcaaac
     1441 ccctctgcat cggagagatc gattcagggt tcatggtggc ctcggagagc gtggccatag
     1501 atacactaaa cgggaagttg ataagggatg tcaagccagg cgagttgata gtgataagga
     1561 acggcgagat gaagtcctat caactggccc gttccacccg gccggctcat tgcatcttcg
     1621 aatatatcta cttcgcccgg ccggatagca ttatggatgg ccgcttgatc tatgatgtgc
     1681 gggtgaacat cggctccaac ctggctcggg agcatccagc ctgtgctgac accgtcaccc
     1741 ccatccccga ctcaggaata actttggccg tgggctacca ccagcattcc ggcataagct
     1801 accgggagtg cctgatgaaa aatcgctaca taggccgaac cttcatcatg cccgatcaga
     1861 acatgaggga gacggcggtg cgcctgaaga tgaacaccat ccggccgaac atcgaggggc
     1921 ataagcttat cctggtggac gactccattg tgcgcggcac caccagccgg aggattgtga
     1981 acatggtccg caaggccgga gcccaggagg tgcacgtccg cataggcagc ccccccatcc
     2041 tcgccccctg ctatctagga atagacatgg ccagccggga tgagctgatc gccgcccata
     2101 agacggt
//
LOCUS       DAOSGP010000241         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_52943_length_2090_cov_14.951843, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000241 DAOSGP010000000
VERSION     DAOSGP010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52943_length_2090_cov_14.951843"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PLV49_06435"
     CDS             complement(<1..1226)
                     /locus_tag="PLV49_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98518.1"
                     /translation="MDVSFSAWRHSREESLQLKGDSFSFLFDRRYTNRLNRMSRVFFE
                     AYVVATKELAAQEDRFPSLSEIDAKMESGAVYAFKDRLMKNTEFFEEVKISGVSYLVP
                     RALKFVDSTGTYTDLILNFDEGAVVLPTRRKTPSLAEPTVPPTEEGEAASFETEEDIE
                     FSAEPEDKAAPEPRPESYSRLFEKAANTTATEEEEADLPEPPVAKAEEAAPPKPSATA
                     TFAPSSGKEPPSLPAKGRMSKEMIVAALAGLIMLMALGGYILSGGFPSNSSSKPLIFV
                     DYSAYLINSSNESYLGFDISNPQGMPNLIEMKLPSNIDESISARGGIVTIAHGTDTLV
                     RMNTSTDASVKIYLLNNWSQIPLTLNFSGTEDYDTSLQVHSQDYLVTRKNDTLILSLN
                     LTGDGVKFEQSYAEKS"
BASE COUNT          488 a          533 c          556 g          513 t
ORIGIN      
        1 taggattttt cggcgtagct ctgctcaaac ttcacgccat ccccagtcag attcaagctc
       61 aagatcaggg tatcattctt gcgagtgaca aggtaatcct ggctatggac ctgcagactg
      121 gtatcataat cctcagtgcc cgaaaagtta agggtcagag ggatctggga ccagttgtta
      181 agcagataga tcttcacgct ggcgtctgtg ctggtgttca tcctcaccag ggtgtcagta
      241 ccatgagcta tggtgacgat tcctcccctg gcgcttatgc tctcatcgat gtttgagggc
      301 agcttcatct caattagatt gggcatccct tgaggattgc ttatgtcaaa gcccaggtag
      361 ctttcgttgc tggaattgat aagataggca gaataatcaa caaaaatcag aggcttgctt
      421 gatgaattgg agggaaagcc gccggataag atatagcctc ccaacgccat cagcatgatc
      481 aaaccagcca gagctgctac tatcatctct ttgctcattc ttcctttcgc agggagcgat
      541 ggtggctctt ttcccgagga tggggcaaag gtggcggtgg cagagggctt tggaggagcg
      601 gcttcttccg ccttcgcgac gggaggttcg ggcaagtcag cttcctcttc ctcagttgcg
      661 gttgtattcg cggccttttc gaatagacgg gaataggatt ccggcctggg ctcaggtgcg
      721 gctttgtctt cgggttccgc tgaaaattct atatcctcct ccgtctcgaa ggaggcagct
      781 tccccttcct cggtgggggg caccgtcggc tcggcaagag agggggtctt cctgcgggtg
      841 gggaggacca ccgccccttc atcgaagttc agaatcagat cggtatacgt tcccgtggaa
      901 tccacgaact tgagggcgcg cggaaccagg taagagacgc cggatatctt gacctcctcg
      961 aagaactcgg tgttcttcat caggcgatcc ttgaatgcat agacggcacc gctctccatc
     1021 tttgcatcga tctcgcttaa gctggggaag cggtcctcct gcgcggccaa ctctttggtg
     1081 gcgacgacat aagcctcgaa gaaaacccgg ctcattcgat tgagcctgtt ggtgtagcgc
     1141 ctatcgaaga ggaaggagaa gctatctcct ttgagctgca agctctcttc gcgcgaatgc
     1201 cgccaggcag agaaagagac gtccattaga tatgcatatc ttcaatccaa ataagtattt
     1261 tatgtatggc cagattgaat ttctccgctc cagagataga aatcgtgatc aatacagtga
     1321 atagatctca tataaatctc gtatgacagc tatctttaaa tctcgccgga aaaaatatgg
     1381 agtcgatgaa acacttggga ttggtgtggg ttcttttgct cctggggatt ggagcgataa
     1441 tgggaccggc tacggcacag ctgacggccg gccagagcgt ccctccatcg ttgaacttgg
     1501 gcgatagtgc aatggttaca gttacgctga cctattacgg caataatgcc actcaggcgg
     1561 taatcactcc cggacttcct ccggggattg agaccagcta tccacaaggc cagacagcag
     1621 agctatatcc tggcatgaca gcgcccatca gctatcccat aagagcaatt cagtctggca
     1681 cgtatattat agcttctcat gtatcgtatt ccgaagaggg aacctggagg aatttgtggc
     1741 tggagtcgcc tctcacagta ataggacagc caacgccgca acccggccag atggatcccg
     1801 ctggcatggg gcctctgttt ggggatagtc cactggggat gaatgaatct gaatcatatc
     1861 cccccgattc tatgccgcca aatcagagca cttgggagcc tatcatcccg gatggcaatg
     1921 gcgatgccta cccagaggat actcctcatt taccggacca gacaccggat cagatgcctg
     1981 aggcagataa gaattgaagc cactgaaatt ctcttatcat tccgcctttt tcttatcgag
     2041 tcccagatcg ctgcgatcat agagcgcctg cagggagaag ccgcgctctt
//
LOCUS       DAOSGP010000242         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_53029_length_2088_cov_16.708805, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000242 DAOSGP010000000
VERSION     DAOSGP010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53029_length_2088_cov_16.708805"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2088
                     /locus_tag="PLV49_06440"
     CDS             <1..>2088
                     /locus_tag="PLV49_06440"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOG98519.1"
                     /translation="AEESKSPNRGMVHNIHGIHGTAKGQKTDDAGEMHKNHQMSMARH
                     PDKSSERHRGRHEGHKTEDFKRRLLISLIITLPILLLSSFIQELFRFELQFPGSLYVV
                     FLLSSLVYFYGGYPFLKGFFKEVKDKNIGMMTLIATAISAAYFYSSAVVFGLMGETFF
                     WELATLIDIMLFGHWIEMRSILGASRALEELVKIMPSEAHLIKDGQTVEVKVETLKPG
                     DRVLVKPGEKIPIDGTVIEGETSVNESMLTGESRPVRKRAGDDVIGGSINGEGSISVK
                     VKKIGSETYLSQIIELVRQAQESKSRTQDLADNAARWLTIISLSVGAITFAAWLNFSK
                     DSAFAIERAVTVMVISCPHALGLAVPLVVAVSTSLAAKSGLLIRDRSAFERARDIQAV
                     VFDKTGTLTEGRFGVTDIIPLAGLNETEVLRMAASLEANSEHPIAAGIVKGSKEMGID
                     LIPIQGFKSIPGKGIEGQVQGRSWMMASPGYLRENNIKLDDDRVNRLAEQGKTVVFLL
                     EEDRLVGAVALADMIRKESREAIEELKSMNINCMMLTGDNRFVARWVAEDLGLDDFFA
                     EVLPHEKAQAIRNVQREYIVAMVGDGVNDAPALVQADVGVAIGAGTDVAVESADIVLV
                     RNDPRDMANIIRLSKRTYSKMFQNLIWATGYNALAIPLAAGVLYGYDIILSPAIGAIL
                     MSASTVIVAINAR"
BASE COUNT          546 a          484 c          597 g          461 t
ORIGIN      
        1 tggcagaaga gagcaagtcg ccaaacaggg gaatggtgca taacatacat ggaatacatg
       61 gcacggccaa aggccaaaag acagatgatg caggcgaaat gcataagaat caccaaatga
      121 gcatggctcg gcatccggac aaatccagcg aaagacaccg tggcagacat gaaggccata
      181 aaaccgagga ttttaaaaga cggttattaa tttctctgat tataacgctg ccgattctgc
      241 tcctctcttc ttttattcag gaattattcc ggttcgaatt gcagtttccg ggctcccttt
      301 atgtggtgtt cctgctctca tcattagttt atttttatgg tggttatccc ttcctgaaag
      361 gatttttcaa agaagtcaag gataaaaata tagggatgat gactctcatc gccaccgcca
      421 tcagtgctgc ttatttttac agttcggcgg tcgtctttgg ccttatgggc gagaccttct
      481 tctgggagct ggccacgctg atcgatatca tgctttttgg ccactggatt gagatgcgtt
      541 caatcctcgg agcttcccga gccctggagg agctggtcaa gatcatgccc tcggaagccc
      601 acttgataaa agatggtcag acggtggagg tcaaggtcga aactcttaag ccgggagacc
      661 gagtcttggt caaacccggg gagaagatac ccatcgacgg aacggtgatc gagggagaga
      721 caagcgtaaa tgagtccatg ctcacaggcg aatccaggcc ggtcaggaaa agagcgggag
      781 acgacgtgat aggcgggtcc atcaatggcg agggctcgat atcggtaaag gttaagaaga
      841 tcggatccga gacctacctc agtcagatca tcgagcttgt caggcaggct caggagagca
      901 aatccaggac tcaggacctg gcagacaatg ccgctcgatg gcttaccata atatctctga
      961 gcgtcggagc tatcacattt gctgcctggc tcaacttcag caaagactcg gcttttgcca
     1021 ttgagagagc agtcacagtg atggtcatat cctgtccaca tgctctgggg ctggcagttc
     1081 ctcttgtggt tgcagtctca acctcactgg ctgcgaaatc aggtctattg atccgggata
     1141 gatccgcttt tgagagggcc agggacatcc aggcggtggt attcgacaag acggggactc
     1201 tgaccgaagg ccgatttggg gtcaccgata tcatacccct tgccggcctg aatgagaccg
     1261 aggtcctgag aatggccgca tctctggaag caaactcgga gcaccccata gcagcgggaa
     1321 ttgtaaaagg ctcaaaagaa atgggcattg atctcattcc catccaggga tttaaatcaa
     1381 ttcccggaaa agggattgaa ggtcaggttc agggaaggag ctggatgatg gcaagcccag
     1441 gttacctgag agagaacaac atcaagctag atgatgatag agtgaacagg ctggcggagc
     1501 aggggaagac agtcgtcttc ttgctggaag aagataggct tgtcggggcc gtagccctgg
     1561 ccgatatgat aagaaaagaa tccagagagg caatcgaaga gctcaagtcg atgaacatca
     1621 actgcatgat gctcaccgga gacaatcgat tcgtcgcccg atgggtggcg gaggatctgg
     1681 gcttggacga cttttttgca gaagtattgc cccatgaaaa ggctcaggcc ataaggaatg
     1741 ttcagaggga atacattgtg gctatggttg gagacggggt gaatgatgca cctgccctgg
     1801 tgcaggctga tgtgggagtc gccatcggag cgggcactga cgttgccgtt gagtcggcgg
     1861 acatagtgct ggtcaggaac gatcccaggg atatggcaaa tatcatccgc ctgtcgaaaa
     1921 ggacttacag caagatgttc cagaacctga tatgggcaac gggctacaac gcactggcca
     1981 tccctctggc cgcaggcgtc ctctacggct atgacataat tctcagcccc gccatcgggg
     2041 ctattctcat gagcgccagc acggtgatag tggctataaa tgcgaggc
//
LOCUS       DAOSGP010000243         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_53431_length_2078_cov_19.805734, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000243 DAOSGP010000000
VERSION     DAOSGP010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53431_length_2078_cov_19.805734"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLV49_06445"
     CDS             complement(<1..299)
                     /locus_tag="PLV49_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98520.1"
                     /translation="MTRCCKCPMPAVIYQKYSGMHLCQSHFDDDVQRKVRETIRQTGL
                     FAHGLRLALAMDGGKGSAVMANIIKDLFARRRDIDLALLILDDHGPGAKAARIAS"
     gene            complement(315..1352)
                     /locus_tag="PLV49_06450"
     CDS             complement(315..1352)
                     /locus_tag="PLV49_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HOG98521.1"
                     /translation="MRVLKRDLRGEEGEIALLPESLDDLWHLRHLVERGDLVFALSQR
                     KVPAIADKARPEKMERKTVRLGVKIEDVEFHMYSNWLRLHGRIVSGMDVGSYHTLNIE
                     VGTDLSILKYHWRPDLLARIDDAVKESQRPKVVLALVEEGEATIGLLRQFGVQMAAEI
                     RMSSGKGSGEDTRASFMRGVAEAMDKTAQGGADVILAGPGFAKDDLKKVIDSSYPDLA
                     KRIAMDDASSIGRSGFQEVLRRGAVKSVLESSRIAREAKLIEELFREIATGGKAAYGI
                     AEVQKAMNYGAIESLLVLDELARRDDVDDIMRQVGNSRGKVIIFSSEFEPGDRLASMG
                     GVAALLRFKIA"
     gene            1410..1497
                     /locus_tag="PLV49_06455"
     tRNA            join(1410..1446,1462..1497)
                     /locus_tag="PLV49_06455"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Asn,seq:gtt)
     gene            1507..1581
                     /locus_tag="PLV49_06460"
     tRNA            1507..1581
                     /locus_tag="PLV49_06460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1542..1544,aa:Ile,seq:cat)
     gene            complement(1669..>2078)
                     /locus_tag="PLV49_06465"
     CDS             complement(1669..>2078)
                     /locus_tag="PLV49_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOG98522.1"
                     /translation="KHGMFHIRIINKGKRPWDLGCPHCNFLDWQAKKALELAEGKESG
                     EGKGAKARPAKKTVRGSGAKAAKKASRPSNADGDGLARLPGIGPKTLEKLAAAGIKTS
                     GELAGANAKSLAAKTGLSEKKIAAWKSAAKALS"
BASE COUNT          370 a          660 c          532 g          516 t
ORIGIN      
        1 tgggaggcga tcctggccgc cttcgcgcca gggccgtggt catcgaggat caggagggcg
       61 aggtcgatat cccttcgcct cgcgaacagg tcctttatga tattggccat aactgcgctg
      121 ccctttcctc catccatggc cagagccagt ctcaggccgt gggcaaagag cccggtctgg
      181 cgaattgttt cccttacctt gcgctggacg tcatcatcaa agtgggattg gcatagatgc
      241 attcctgagt acttctgata gattacggca ggcataggac acttgcagca tctggtcatt
      301 tcatccccct ttgatcatgc tatcttgaac ctgagcaatg ccgccactcc tcccattgag
      361 gccagcctat ccccgggctc aaactcagag ctgaatatga tcaccttgcc gcgggaattg
      421 cctacctggc gcatgatatc gtccacatca tcccggcggg cgagttcatc caagaccagc
      481 aggctctcta tggctccgta attcatcgcc ttctgcacct cggcgatgcc gtaggccgct
      541 ttgccgcccg tagcgatctc cctgaatagc tcctcgatca gctttgcctc cctggctatg
      601 cggctggact caaggacgct tttcaccgct ccccggcgga gcacctcctg gaagcccgat
      661 ctgccaattg aggaggcatc gtccatggcg atcctctttg ccagatcggg gtaagaggag
      721 tctatcacct tcttcaggtc gtccttggca aatcccggac ctgcgaggat cacgtccgct
      781 cccccctggg ccgttttatc catggcctcg gccacccctc tcataaacga ggcccgggtg
      841 tcctcgccgc tccctttgcc gctgctcatg cgaatctctg ctgccatctg caccccgaac
      901 tggcggagaa ggcctatggt ggcctctccc tcctccacca gggccagcac caccttgggc
      961 cgttgcgatt ctttcacagc atcatcgatt cgtgccaaga ggtctggcct ccagtgatat
     1021 ttgagtatgg acaggtccgt tcccacctcg atattcaggg tgtggtacga tcctacatcc
     1081 atgcccgata ctatgcgtcc atgcagccgc agccagttgg agtacatgtg aaactctaca
     1141 tcttctatct tgacgcccag gcgtaccgtc tttctctcca tcttctccgg cctggccttg
     1201 tcagcaatcg ctggaacctt tctctggctc agggcgaata ccaggtctcc cctctccact
     1261 aagtggcgca ggtgccacag gtcatccagg gactcgggaa gaagggcgat ctcgccctcc
     1321 tcgccacgca aatctctctt caggacgcgc atgagatatc ctctctaatc aaaaggctta
     1381 aatcattgtg catccagggt ggtggtcgag ccgccataac tcagatggta gagtgtctgg
     1441 ctgttaattg aataagcagc aaccagaatg tcacaggttc gagccctgtt ggcggcgcat
     1501 tcctctgggc ctgtagctta gtccggttag agcgctcggc tcataaccga gtggccgccg
     1561 gttcaaatcc ggccaggccc atgctggcaa ctatagccat cttggccttt cctgaatttc
     1621 atatctttga aaagccgaaa aaattggtga tgagggatat agcgcgtctc agctcagggc
     1681 tttggctgcg ctcttccagg cggcgatctt cttctcggac aggccggtct tggcggcgag
     1741 gcttttggca ttggcacctg ccagctctcc agatgtcttg atccctgccg ctgccagctt
     1801 ttccagggtc ttgggcccaa ttcccggcaa tctggcgagc ccatcgccat cagcattgga
     1861 tggcctgctg gccttcttgg cagctttggc ccctgatccc ctgaccgtct tcttggctgg
     1921 ccgcgctttt gccccttttc cttccccgct ctccttgcct tctgccaatt ccagggcctt
     1981 cttcgcctgc cagtctaaga agttgcagtg ggggcagccc aggtcccagg gcctcttgcc
     2041 cttgtttatg atgcggatat gaaacatgcc gtgcttct
//
LOCUS       DAOSGP010000244         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_53723_length_2070_cov_14.607444, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000244 DAOSGP010000000
VERSION     DAOSGP010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53723_length_2070_cov_14.607444"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLV49_06470"
     CDS             complement(<1..447)
                     /locus_tag="PLV49_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG98523.1"
                     /translation="MLIRARTIADYQFGRGAGSALFPDGTTYSLSKTRRLRYLYWEEE
                     RVATVRARDNLLTLSMIGAKRLHDLFCSPRLRVVASDDAAPFIAKGGNLFARHVIAVD
                     PEIRAGEEVLVVDSQDRLLATGTAVLAPEEMMQIKRGLAVQARKAAE"
     gene            586..1830
                     /gene="glyA"
                     /locus_tag="PLV49_06475"
     CDS             586..1830
                     /gene="glyA"
                     /locus_tag="PLV49_06475"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG98524.1"
                     /translation="MKSLDIVDPEIAAAIQSELERQRNNIILIASENFTSRAVMEAQS
                     CVMTNKYAEGYPGKRYYRGTGCVDLAENLARERCIKLFGAEHVNVQPHSGTQANMAAY
                     FAVLNAGDTIMGMNLAHGGHLSHGSPVNFSGKMYKIVPYNVSRETEMLDYSEMADIAR
                     KSRPQLIVVGASSYPRTIDFKAVREIADDVGAKVMADIAHIAGMCAVGAHPSPVPYAD
                     IVTTTTHKTLRGPRGAIILCKEELASAIDRSVFPGTQGGPFMNSIAAKAVAFKEALTP
                     EFKEYQFQVIRNARALAERLLKNDFDLVSGGTDNHLLLVKLLKEGITGKVADETLERA
                     GITLNKNMIPFDPATPFITSGIRIGTPAMTTRGMKEKQMAQIADMITRVLRDINNEKT
                     IATVRSEAKSLCDQFPLYPDMV"
     gene            1827..>2070
                     /locus_tag="PLV49_06480"
     CDS             1827..>2070
                     /locus_tag="PLV49_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOG98525.1"
                     /translation="MIKLATIIDGKALAGEVETETLQRAKKLATKGIVPGLATVLVGE
                     NPASQMYIRLKHSACSRAGIRSENVLLPESSTEEEII"
BASE COUNT          486 a          604 c          569 g          411 t
ORIGIN      
        1 ctctgccgcc tttctcgcct ggaccgccag ccccctttta atctgcatca tctcctcagg
       61 cgcgaggacg gctgtgcccg tggctaaaag ccggtcctgc gagtcaacaa ccaaaacctc
      121 ctcaccggct cgaatctccg ggtcaacggc aatgacgtgc ctggcgaata gattgccacc
      181 tttggcgatg aatggcgcag catcatccga ggccaccact cgaagcctag ggctgcagaa
      241 cagatcatgc agcctctttg cgccgatcat gctcagggtc aggaggttgt cccttgctcg
      301 gacagtggcc accctctcct cttcccagta gagatatctc agccggcgag tcttggagag
      361 gctgtatgtc gtgccatcgg gaaagagggc actgcccgcg cccctgccaa attggtagtc
      421 agcgatagtc ctcgctctta taagcatcca gattaccttt gcaggaggca ataaaagagt
      481 tatggtgcca gtgccagagc atcatatctc gggctcatac cataatctat tttgccctcc
      541 tcagtaaaca ccacccatct cgctaattag caccggtgat tcgatttgaa atccctagat
      601 attgtagatc ccgaaatcgc agccgcgatc cagtctgagc tggagaggca gcgaaataat
      661 atcatcctga ttgcatcaga gaacttcacc agtcgagcgg tcatggaggc gcagagctgt
      721 gtcatgacca ataagtatgc cgaaggctat cccggcaaac gctactatcg cggcactggc
      781 tgcgtggacc tggccgagaa tctggcgaga gagcgctgca ttaagctctt tggcgcagag
      841 catgtgaacg tccagcccca tagcggcacc caggccaaca tggctgccta cttcgccgta
      901 ctcaacgcgg gcgataccat catgggcatg aacctcgctc acggcggcca cctctcccac
      961 ggcagcccgg tcaacttctc aggcaagatg tacaagatcg tcccttataa cgtctcccga
     1021 gagacggaga tgctcgacta ctccgagatg gcggacatcg cccgaaagtc ccggccccag
     1081 ctcattgtgg tgggcgcatc cagctatccc cggaccatcg acttcaaggc ggtgagggag
     1141 atcgccgatg atgtgggggc taaggttatg gcggatatcg cccatatcgc cggcatgtgt
     1201 gcagtgggtg ctcatccctc ccccgtgccc tatgcggata ttgtcaccac caccactcat
     1261 aagaccctgc ggggtccgag aggcgcaata atcctctgca aagaggagct tgcttcggcc
     1321 attgaccgct ctgtttttcc cgggacccag ggggggccgt tcatgaactc catagcagca
     1381 aaagcggtgg cattcaagga ggccttgacc ccggagttca aggaatatca gttccaggtg
     1441 atcaggaatg cccgggcttt ggctgagcgt ctgctgaaga acgacttcga tctggtcagc
     1501 ggtgggacag ataaccattt gctcctggtc aagctgctga aagagggcat caccggaaag
     1561 gtggctgacg agactttgga gagggcagga atcaccctga acaagaacat gatacccttc
     1621 gacccggcca cgccattcat caccagcggc attcggattg gcaccccggc catgaccacc
     1681 agaggcatga aggagaagca gatggcccag atagccgata tgatcaccag ggtgctccgc
     1741 gacatcaaca atgagaagac catagctacg gttcgctctg aggccaagtc cctctgcgac
     1801 cagttccccc tgtatccaga tatggtgtga tcaaactggc aactataata gatggaaagg
     1861 ctctggccgg cgaggtggag acggagacgc tccagagggc gaaaaagctt gctacaaagg
     1921 ggatcgtccc cggcctggct acagttctgg tgggagagaa tccggcctca cagatgtaca
     1981 tccgtctaaa gcactccgct tgctcccggg caggaataag atccgagaac gtgctcttgc
     2041 cggaaagcag cacagaagag gagatcatcg
//
LOCUS       DAOSGP010000245         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_54324_length_2055_cov_16.599000, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000245 DAOSGP010000000
VERSION     DAOSGP010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54324_length_2055_cov_16.599000"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLV49_06485"
     CDS             complement(<1..446)
                     /locus_tag="PLV49_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOG98526.1"
                     /translation="MATRIAVTGSPGIGKSTLVAKVISGTKLRVGGVLARDRCYKDRR
                     TGFELLDLSTGMVGILADEAGDGPQLGKYRVHLDDLDRIGAQAVENALGCDLIVVDEV
                     GPMELSSHRFVLAVEKAIASPKPMLVVLHQWSNHRLAKKIRGSFRV"
     gene            complement(436..876)
                     /locus_tag="PLV49_06490"
     CDS             complement(436..876)
                     /locus_tag="PLV49_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HOG98527.1"
                     /translation="MKGSIRDREREECKDKGTYDLEALEELLAALVEASGQGAATIVE
                     GRRDELALRALGLTGPVIMASRRSALDLAEDAARSYREIILLTDWDRKGDEMARTIEQ
                     HLWSVGSRPDGEIRSRIKKLVRKEIKDVESLHRYMERMRYLYGH"
     gene            complement(878..1207)
                     /locus_tag="PLV49_06495"
     CDS             complement(878..1207)
                     /locus_tag="PLV49_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOG98528.1"
                     /translation="MDYAEALDSHPLGCIIRFEVVPGSSRLAVPSGFNPWRRSLEARL
                     TEKPSRGRANRQLVEELARTLGVAESGIEVIKGEKSGRKLLLVKGIEKDRAVLILKPL
                     LNQQRGR"
     gene            1338..>2055
                     /locus_tag="PLV49_06500"
     CDS             1338..>2055
                     /locus_tag="PLV49_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOG98529.1"
                     /translation="MKIKQVVVLAGKDHIDCSISSGLEQLGLQMCECFSPQDHKEDCL
                     LVCDQDYADTSNGSALIRQAMERGCAVLLHNSNCPSVLEEIRSTGDLSQKTRDMLEAQ
                     VLYRCLQKDIQRLASLQPEADGLPNYVASAYQKVQDLRYGENWHQRAALYINDQGLGL
                     PAARKLNGREMSYNNMVDAETVLEMLIDLSDYDGEACNRPLSVIVKHANPCGVAYGTS
                     LDEAYRLSFATDPKSAFGGVI"
BASE COUNT          431 a          622 c          541 g          461 t
ORIGIN      
        1 aatacccgga aggatccccg gatcttcttt gccaggcggt ggttggacca ctggtgcagc
       61 accaccagca tgggcttggg ggaggcgatg gccttctcca ccgcaaggac aaacctgtga
      121 ctggaaagct ccatggggcc cacctcatcc accactatca aatcgcagcc aagagcattt
      181 tccaccgcct gcgccccgat ccggtccagg tcatccagat ggacccggta ctttcccagc
      241 tgcggaccgt cccctgcctc atccgccagt atccccacca ttcctgtgga caggtccaaa
      301 agctcaaagc cagttcgtcg atccttgtag cacctgtccc tggccagcac cccgccaact
      361 ctcaatttcg tcccggagat cacctttgcc accagagttg atttgccgat tccagggctg
      421 ccggtgactg caattctagt ggccatacag atacctcatc ctttccatgt aacgatgcag
      481 gctttccacg tccttaatct cctttcggac cagcttcttg atgcgagagc ggatctcccc
      541 atcgggacgg gaacccaccg accacagatg ctgctcgatg gtcctggcca tctcatcccc
      601 tttgcgatcc caatcggtga gtaggatgat ctcccggtag ctccgggcag catcctcggc
      661 caggtcgagg gccgatcggc gcgaggccat gatcaccggc ccggtcaggc ccagggcgcg
      721 cagggccagt tcatcccgcc tgccctcaac gatagtcgcc gccccctggc ccgatgcctc
      781 gaccagggca gccagcagct cttctaaggc ttccagatca taggtcccct tatccttgca
      841 ctcctctcgc tctctgtccc tgatcgatcc cttcatttca tcttcctctc tgttgattca
      901 gcagaggctt gaggattaag accgctctat ccttctctat tcccttaacc agcagaagct
      961 ttcgcccgct cttctctcct ttaatcacct cgattccgga ttcggccacg cccaatgtcc
     1021 tcgccagctc ctcgaccagc tggcggtttg ccctgcctcg agatggcttt tcagtcagcc
     1081 gggcttctaa agacctcctc cagggattga agcccgaagg aacggccagc cgggatgagc
     1141 caggcaccac ttcaaagcgg atgatacatc ccagaggatg agaatcgagt gcctcagcat
     1201 agtccatgct ctttataatg ctctgggagt ttaaatattg tcgatcacaa tctgctgatg
     1261 ggaggacatt gcaggggtgt tcctgtgtta atgggaaaag gttaaaatac cgttagcgat
     1321 gaagggggag aggttcgatg aagatcaaac aggtcgtcgt actggcaggc aaggaccaca
     1381 ttgattgctc catttcctcg ggcttagagc agcttggcct gcagatgtgc gaatgctttt
     1441 ctccccaaga tcataaagag gattgtctcc tggtatgcga tcaggactat gcggatacct
     1501 cgaatggctc tgccctcatt aggcaggcga tggaaagagg ctgtgcggtg ctattgcata
     1561 actcgaattg cccctccgtc ttggaggaga tcaggagtac gggcgacctc tcccagaaga
     1621 cgagagacat gctggaggct caggtgctgt accggtgcct gcagaaggat atccagaggc
     1681 tggccagcct tcagcctgaa gcggatggcc tgccaaatta cgtggcgtct gcctatcaga
     1741 aggtgcagga cctgcgctat ggagagaact ggcatcagag ggcagctctt tacataaatg
     1801 accagggtct ggggctgccc gccgcccgga agctgaacgg ccgggagatg tcctacaata
     1861 atatggtcga tgcagaaacg gtcttggaga tgctcatcga cctctcggat tacgatgggg
     1921 aagcctgcaa taggccactc tctgtcattg taaagcatgc caatccctgc ggcgtggcct
     1981 atggcacaag ccttgatgag gcctaccgac tctcctttgc caccgatccc aagtctgcat
     2041 tcggtggggt gatag
//
LOCUS       DAOSGP010000246         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_54572_length_2049_cov_11.348546, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000246 DAOSGP010000000
VERSION     DAOSGP010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54572_length_2049_cov_11.348546"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2049)
                     /locus_tag="PLV49_06505"
     CDS             complement(<1..>2049)
                     /locus_tag="PLV49_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOG98530.1"
                     /translation="LDDLGVKDAWDMGYTGKGVNIAIIDFGIDFATPDLIGTQARVSN
                     VSSPYYGWPIVIDLESLSYYQDGTSGFSSHYANTTSMDVKDYQVTGTSKSGIYHIGDH
                     PDQHLAQFYGQPVKVLVVDESVASVYDTVYVDLNNNHDFGDDKPCRKGDEASYWDRDN
                     DGYPDESGGMVYFIADGKTPLPLSRMIYGEKAKIPKNGELIAFHCDDDIHGTMCASII
                     AAQGKNIMGIAPDARIISVRLGKTEELLWLLAVLGYDGVPNTGDEADVISRSGGFMYR
                     LNKGADEGSAFLEYLSTNVSPSTTMIFVSGNDGSGYGTCNPPSGEHAINVGATYDQWW
                     NSSPYRGDVTCFSSRGPNALGQVKPNVLAPGYRTPESLPLWITHSGKAAWDNHGGGTS
                     GATPHAAAVVALIYQAYKEAHGEFPTSEKARDILMSSATDINEEIFAQGSGMINAAKA
                     VEIASGRNGVLIEPALLVTTPVEAGSTLKFNFTLTNHSGRTVSLKPQILIKDKTWEFT
                     QSSANESILAIPEDMLACNLLKVSSYYSRDARNTKLEKNEGYDIYLYNWEDMNEDGEI
                     QNDELEIVALNTVAWGYGFSSEARMHDPSKRVNDGLAVVLKKRGEIQSNETKAVVEAY
                     NWTPWDIGMNINGSKMSVSIPVPNTTGVFQGKLLLEGDDGRQCIPISFSAYKHDDISL
                     S"
BASE COUNT          508 a          523 c          436 g          582 t
ORIGIN      
        1 gcttaacgag atatcatcgt gtttataggc tgagaaggat atcggaatac actgtctccc
       61 atcatctccc tctagaagga gctttccctg aaatacgcct gtagtattgg gtactggtat
      121 tgaaacactc atcttgctgc cattaatatt cattccgata tcccatggcg tccaattata
      181 tgcttcgacc actgcttttg tctcattgct ctgtatctcg cccctcttct tgagaacaac
      241 agcaagtcca tcattcactc gctttgacgg atcatgcatt cttgcttcag acgaaaatcc
      301 gtaaccccaa gcaacagtat tcagcgctac gatttccagt tcatcatttt gtatttctcc
      361 atcctcattc atatcttccc agttgtagag gtatatgtca tagccttcat ttttctccag
      421 ctttgtattc ctggcatccc tggaataata ggaggaaacc ttcagcaggt tgcaggcgag
      481 catatcttca gggatagcta aaatgctctc attcgccgag ctttgtgtaa attcccacgt
      541 tttgtcttta atcaggatct ggggctttag actaaccgtt ctgccagaat gattcgtcag
      601 agtgaagttg aactttagag tggatccggc ttcaactgga gtcgtgacca gaagggccgg
      661 ttcaatcaga actccgttcc ttcctgatgc gatctccact gcttttgcgg cgtttatcat
      721 gcccgatccc tgagcgaaga tctcttcatt gatgtcggta gctgaggaca tgagaatatc
      781 cctggccttc tcagaggtcg gaaactctcc atgagcttct ttgtaagcct ggtagatcaa
      841 agcgacaact gcagccgcat ggggtgttgc accgctggtc ccgccgccat gattgtccca
      901 tgcagctttg ccgctgtgag ttatccataa aggcagagat tcgggcgtac ggtagccagg
      961 ggccagtacg ttcggtttga cctgacccag agcgttcgga ccgcgagaag aaaaacaggt
     1021 tacatctcct cgataagggg agctattcca ccattgatca taggttgcgc ccacatttat
     1081 cgcatgctct cctgaaggtg ggttgcaggt accataccct gaaccgtcgt ttccacttac
     1141 aaatatcata gttgtggacg gagagacgtt tgtggagaga tattctaaga atgctgagcc
     1201 ctcatcagca cctttgttaa ggcgatacat gaaacctccg cttcgactta taacgtctgc
     1261 ctcatctccc gtattcggaa ctccatcata gccaagcaca gccagcaacc agagaagctc
     1321 ctcagttttt cccagtcgaa cagatattat cctggcatca ggggcgatgc ccataatatt
     1381 tttgccctgg gccgctatta ttgaagcgca catggttcca tgaatatcat catcgcaatg
     1441 aaatgcaatt aactctccat tctttggtat ctttgctttc tcaccataga tcatccttga
     1501 taaaggcaga ggcgtcttgc catcagctat aaagtagacc atgccaccag attcatctgg
     1561 atagccatcg ttatctctat cccaatagga ggcctcatcg ccttttctgc aaggcttatc
     1621 atccccgaaa tcatggttgt tgtttaaatc aacgtatact gtatcataaa cgctagcgac
     1681 actctcatcg accacgagaa ccttcactgg ctggccataa aactgagcaa ggtgctggtc
     1741 gggatgatct ccaatatggt aaatcccgct cttactggtt cctgttacct gataatcctt
     1801 tacatccatc gaggtagtat ttgcataatg agaactaaag ccagaggttc catcctgata
     1861 ataggagaga gactccagat ctattacaat gggccagcca tagtaaggag agcttacatt
     1921 tgagacacgg gcttgagttc ctatgaggtc tggagtagcg aagtctatgc caaagtctat
     1981 tatcgctata ttcactcctt tgcccgtgta gcccatatcc catgcatcct ttacaccaag
     2041 gtcatccag
//
LOCUS       DAOSGP010000247         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_55021_length_2038_cov_12.385779, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000247 DAOSGP010000000
VERSION     DAOSGP010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55021_length_2038_cov_12.385779"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..505)
                     /locus_tag="PLV49_06510"
     CDS             complement(158..505)
                     /locus_tag="PLV49_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98531.1"
                     /translation="MPGFSWANDKLAPYDYDAEKARDLLGPGRIEGHRWRWPGGQGRQ
                     ATGAIALHMAAEARTAAHGYSNPEVDRLLNDASKTKDQEKRLEYFNQVQEIFQEELPF
                     ITVAYFLFFFVHI"
     gene            complement(630..1406)
                     /locus_tag="PLV49_06515"
     CDS             complement(630..1406)
                     /locus_tag="PLV49_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG98532.1"
                     /translation="MLSDLMPLTDSEVKSAIRSRWDVSSQKYDTHDGHGIKSAEERDA
                     WKRVLGESLARSGLDILDVGCGTGEMSLVLAEMGHNVQGVDLSEKMLSRAREKAKVSR
                     LKVKFSLGDAERLKFDPGKFDALISRHVLWTLPHPDRAMEEWKRVVKVGGQVLVIDAR
                     WRDDSISSRSRRLASEVGMIFLERQNPWKGWYPKDLNSALPHRYGLTSQEAKRYFERS
                     GLKDVTLTDLHDIREIQKVGMPFYRRISFNWTYYMVKGTK"
     gene            complement(1472..>2038)
                     /locus_tag="PLV49_06520"
     CDS             complement(1472..>2038)
                     /locus_tag="PLV49_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98533.1"
                     /translation="DLLANFFVAGIGILAARMMLAPRESMTTKGDETTSAGDWKVILR
                     EGLHECISSSIKITRVLVPTIFLAQLAIEYVLVLPIVERYNAAIEPLGFSSSSLIVLM
                     ASVVSQSAALVASGTLLKEGSLNTVSCLILLFMARFLHLGIGCFRIGIPANVSYFRGS
                     LGLRVTAIEYSLVEVANILMILMIFLFL"
BASE COUNT          518 a          546 c          461 g          513 t
ORIGIN      
        1 tccacctata gcagtctctc cataagcaac gtccaaaaat tgtagtacct agtaataaat
       61 gttttgatca taataatacc aaatgctatt aattttgata taatagtatt taatcatctg
      121 cggaccgaga attgagcact tgatgcaaaa gacaggttta tatgtgtacg aaaaagaata
      181 aaaaataagc cacggtaatg aaaggaagct cctcctgaaa gatctcttgc acctggttga
      241 agtactcgag ccttttctcc tggtccttgg tcttggaggc atcattcagc agtcggtcga
      301 cctctgggtt ggagtatcca tgggcggctg tccgggcctc tgcggccatg tgaagagcga
      361 tagctccagt ggcttgccgt ccttgtccac caggccatcg ccaccggtgt ccttcaatcc
      421 tgcctgggcc caacaaatcc ctggcttttt ctgcatcgta atcataaggt gcgagcttat
      481 cattcgccca gctgaagccg ggcatgaagg gacctatggc cgcctttccc cttatcagaa
      541 tttgattctc ctgcaactgg cccgacggtg agcagagtca gcattataag gaaaaaaggc
      601 ctatcaagtc tcattacaat tcacctttgc tattttgtgc cctttaccat gtagtatgtc
      661 cagttgaagg agatccggcg ataaaatggc ataccgacct tttggatctc tcggatatca
      721 tgcagatcgg tgagggtcac atccttcaag ccggatctct caaagtacct cttcgcctcc
      781 tgggaggtca atccgtagcg atgagggaga gcggagttca gatccttcgg gtaccagcct
      841 ttccaggggt tctgcctctc caagaagatc attcccacct cagaggccag ccttctcgac
      901 cggctggaga tcgagtcatc tcgccatcgc gcgtcgatga ccagcacctg gcctcccacc
      961 ttcaccactc gcttccactc ttccattgcc ctgtcaggat ggggcagggt ccagagcaca
     1021 tgtcggctga tcagggcatc gaatttgcca ggatcgaatt tcagtctctc tgcgtctcct
     1081 aaagagaact tgaccttcag gcgagagacc ttggccttct ccctcgctct ggagagcatt
     1141 ttttcggata gatcgactcc ctgaacattg tggcccatct cagccaaaac caggctcatc
     1201 tcccctgtgc cacaacccac atccaggata tcaaggccgc ttctagcgag agattctccc
     1261 aatactctct tccaggcatc cctctcctct gcgctcttta tgccgtgacc atcatgcgta
     1321 tcatacttct gggacgagac atcccatcga gagcgtatgg cgctcttcac ctctgagtcg
     1381 gttaagggca tgagatctga aagcatattc tctcatgtta aatctttcca tgattgtaac
     1441 ataaaatctt atataaagca tcggtgtaat gctataaaaa taaaaatatc attaatatca
     1501 tcaagatatt agccacttct accagtgaat actctatggc ggttaccctc agaccgagag
     1561 agccccggaa ataggataca tttgcaggaa tgccgatccg gaagcaccca atgccaaggt
     1621 gcagaaacct tgccatgaaa agcaagataa ggcaactaac agtattgaga gagccctcct
     1681 tgagcaatgt gccagaggcc accagtgcgg cactctggct gaccacgctg gccataagaa
     1741 ctatcagact ggacgatgaa aatccaagag gttcgattgc ggcattgtac cgctccacaa
     1801 tgggcagcac cagaacatac tcgatggcca attgagccag aaagatggtg gggaccagca
     1861 ctctggtgat cttgatggat gaggatatgc actcatgcag accttctctt agtatgacct
     1921 tccaatctcc tgcagatgtg gtctcatctc ctttcgtcgt cattgattcc ctgggtgcaa
     1981 gcatcattct ggcggcaaga atcccaatgc ctgccacgaa gaagttggcc agtagatc
//
LOCUS       DAOSGP010000248         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_55342_length_2030_cov_15.594430, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000248 DAOSGP010000000
VERSION     DAOSGP010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55342_length_2030_cov_15.594430"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLV49_06525"
     CDS             <1..315
                     /locus_tag="PLV49_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98534.1"
                     /translation="LPDFWKQIALLNIASMLRPGGRLCLRDIVYSFDPGDYERQIERF
                     ISKTTEKSGPGFAQRMTSHINKEFSTMDWIMRGLIERAGFAIEREEHKEGFLGLYLCR
                     KI"
     gene            complement(340..741)
                     /locus_tag="PLV49_06530"
     CDS             complement(340..741)
                     /locus_tag="PLV49_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98535.1"
                     /translation="MLALKKPALSLALMLFILAGSVQAQLSDGVEVYLGELDLLANIN
                     STNSSLINSTLINSTAANNSTSIDLVLAPPQNLSASTGKEVLDLTSYARDRVNKSLAG
                     YTNIMYPITGSRGTTASTSGGGGGGGGCGCG"
     gene            complement(846..>2030)
                     /gene="acsC"
                     /locus_tag="PLV49_06535"
     CDS             complement(846..>2030)
                     /gene="acsC"
                     /locus_tag="PLV49_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HOG98536.1"
                     /translation="QVKVGGEDVMYRHQMTFFNKPPFAYDVADNMEEAKLIERVKKIT
                     TWRKFYIGKWQNVEMIAVRSVTDDPAKFAACVKTVMANGNGFPLILCSFNPAVLKAGL
                     EVASKEKPLIYAATKDNWKEVAQLAYDYKVPVTLSVPFDLDGLKSMAVTFAEMGLTDL
                     VLDPGTAPNGKMLQQTLTNFINLRRAAVEEAQRDIAYPVMVLPINAWLTTDDPVRAAY
                     WESVLTAAFVIRGGAIMIKHSTEPYSMMPDMHLRFNIFTDPRTPVQVKPGIYKVGNPG
                     PESPVFLTTNFALTYYTVESDVASNGIDAYIIAINTDGIGVQASVAGGQLTPVKIQDS
                     FNEAGFDWAGQKFPAMVLPGMAAKFSGELEDLFAGKAKIMVGPEDSGRIIGWMKDFWP
                     PK"
BASE COUNT          449 a          510 c          593 g          478 t
ORIGIN      
        1 ctgccagatt tttggaaaca gatcgcattg ctgaacatag ccagcatgct gcggccggga
       61 ggaaggctct gtctgaggga catcgtctat tccttcgatc caggcgacta tgagaggcag
      121 atagagaggt tcatatctaa aacgacggag aagtcgggac ctggatttgc ccagagaatg
      181 accagccaca tcaataagga attctccacc atggactgga tcatgcgcgg cttgatcgag
      241 agagcgggat ttgcgatcga aagggaggag cataaagagg gatttctggg tctttatttg
      301 tgcaggaaga tctgaataaa ataaaaaaaa tgctattttt tatccgcagc cgcatccgcc
      361 tccaccgccg ccgcctcctg aggtagaagc agtggtcccc cgagagccgg tgatgggata
      421 catgatattc gtgtagccgg ccaggctctt gtttaccctg tctcttgcat agctggtgag
      481 gtcaagaacc tcctttccgg tggaggctga aaggttctga ggcggggcaa gaactaaatc
      541 gatcgatgtg gagttattgg ccgctgtgct atttatgagg gtgctgttta tcaagctgga
      601 attcgtggag ttgatatttg ccagcagatc gagttcaccc agatagacct ccaccccatc
      661 agacagctgg gcctggacgc ttcctgccag aatgaagagc atcagagcca ggcttaaagc
      721 aggttttttt agggctaaca tagaagatcg tttccctccc gaaattgatt ttcagactag
      781 gaagccaaaa ataagagtat gtgcccgctt tggggcacat gcaatacccc tttttagagt
      841 ccgctctact tgggtggcca gaagtccttc atccagccga tgatgcgtcc ggagtcctct
      901 gggccgacca tgatcttggc cttgcctgcg aagaggtcct ccaattcgcc tgagaacttg
      961 gcagccattc cgggcaggac catggccggg aacttctgac cggcccagtc gaatccagcc
     1021 tcgttgaagg agtcctggat cttgaccgga gtgagctggc cgccagcgac agatgcctgc
     1081 acgccgatac catcggtgtt gatggcgatg atgtaagcat cgatgccgtt tgaggccacg
     1141 tccgactcca ctgtgtagta ggtcagggcg aagttggtgg tcaggaagac tggcgactcc
     1201 ggtccgggat tgccgacctt gtagatgccg ggcttgacct gcaccggagt tctcggatcg
     1261 gtgaagatgt tgaagcgcag atgcatatcg ggcatcatgc tgtagggctc tgtggagtgc
     1321 ttgatcatga tcgcaccgcc gcgaatgacg aaggctgcgg tgagcactga ctcccagtaa
     1381 gcagcacgaa ccggatcatc ggtggtcagc caggcgttga tgggcaggac cattaccgga
     1441 taggcgatat ccctctgggc ctcttcgact gccgcccttc tcaggttgat gaaattggtg
     1501 agggtctgct gcagcatctt gccgttgggt gcggttccgg ggtctaagac caagtcggtc
     1561 agtcccattt cggcgaatgt aacggccatg gacttgaggc catccaggtc gaagggaacg
     1621 ctcaatgtga ctgggacctt atagtcatat gctagctggg caacctcctt ccagttgtcc
     1681 ttggttgcgg catagatgag tggcttctcc ttggatgcga cctccaagcc tgccttgagg
     1741 acagcagggt tgaaggagca gaggatgagc gggaagccat tgccattggc catgactgtc
     1801 ttgacgcagg cagcgaactt ggccggatcg tcggtcacag accggacggc aatcatctcc
     1861 acgttctgcc acttgccgat gtagaacttt cgccaggtgg ttatcttctt gacccgctct
     1921 atgagctttg cctcttccat gttgtcggca acatcatagg caaatggcgg cttattgaag
     1981 aaggtcatct gatggcggta catgacgtcc tcgccaccta ccttgacctg
//
LOCUS       DAOSGP010000249         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_55736_length_2021_cov_20.054934, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000249 DAOSGP010000000
VERSION     DAOSGP010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55736_length_2021_cov_20.054934"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PLV49_06540"
     CDS             <1..986
                     /locus_tag="PLV49_06540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG98537.1"
                     /translation="DPLVSIITPTYNHENFISECLESVLAQSYARWEQIVIDDGSTDK
                     TAEIVSKFKDDRIKYIRQDNKGILRLGESYNLALQISKGEYIAVLEGDDFWPSDKLEI
                     QIDAMRGSDAVLSWGRAEVMDEKGDLLAVLPNDMERFMGLSREEQLGNLLIENPMHSC
                     TIVCKKVALQSIGGFKQPQGLPFVDGSTWLELSLIGDFLPIDAVLGCYRRHDSQISST
                     MKSSMVRAGLYFTEFFKNLPADVRASLAHEDNDIAARMDRKAREIDYYLGRAYLRERK
                     WSDARESLLKAIKEDNLPSIKAKAILGILCGLLRTDLEWLAAFIDPREGRR"
     gene            complement(1035..>2021)
                     /locus_tag="PLV49_06545"
     CDS             complement(1035..>2021)
                     /locus_tag="PLV49_06545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG98538.1"
                     /translation="MKIVLVYYGYFSSFIQKDLDMLSRHYEVEVVSLSSLGDAVKLAG
                     AMIRCRMALIWFAGGHAAATVLLSKLLRKKTIIVVGGFDVACIPEINYGRFAQGRIKS
                     ILTKFALKYADRVLVVDPSLKEDAIKNAGINGHNIDYLPSGFSDTEFYPKGEKEPLAL
                     TVAMGESWDRVLIKGLDVFVESAKHLPEAGFKVVGIKGDALSRLKEIAPDNVEFTGFV
                     THDDLIESYQRARVFCQLSMREGLPNALCEAMLCECIPVGTDRNGIPRAIGDTGFIVP
                     YGDPRAAAEAIKKALADRSNRGKKARERIIELFPEKRREDGLIKTINELAEG"
BASE COUNT          508 a          509 c          493 g          511 t
ORIGIN      
        1 tcgatccttt ggtgtcaata attactccta catacaacca tgaaaatttc atctcagagt
       61 gcctggagag cgtcctcgcc cagagctatg cccgctggga gcagatagtg atcgacgatg
      121 ggtccaccga taagactgcc gagatagtct ccaagtttaa agatgataga atcaaatata
      181 ttcgtcaaga taataagggc attcttaggc ttggcgagag ctacaatttg gcccttcaga
      241 tctcaaaggg cgagtacata gccgttcttg agggggatga cttttggcct tctgataagc
      301 tggagatcca gatcgatgcc atgaggggca gtgatgccgt cttgagctgg gggagggcag
      361 aggtgatgga cgaaaagggc gatcttcttg ccgtccttcc caatgatatg gagcgcttta
      421 tgggtctgtc cagggaagag cagttgggta atctgctcat cgaaaatccc atgcattcct
      481 gcaccattgt ctgcaaaaag gttgcattgc agtcgattgg aggattcaag cagccccaag
      541 gacttccttt cgtggacgga tcgacatggc tggagcttag ccttatagga gatttcttgc
      601 ccatagatgc cgttttgggc tgctaccgaa ggcatgatag ccagatatca tcaaccatga
      661 aaagctcgat ggtcagggca gggctctact tcacagaatt ctttaaaaat cttcctgcgg
      721 atgtcagggc atctcttgcc catgaggaca atgatattgc tgccagaatg gatcggaagg
      781 cgagggaaat cgattactat ctgggaagag cctacttgag agaaaggaaa tggtctgatg
      841 cccgggaatc tctcctaaag gccatcaagg aggataatct tccctctatc aaggcaaaag
      901 ccattttggg gatcttatgc ggcctgctga ggacggatct ggagtggctg gctgcattca
      961 tcgatccaag agagggccgg agatgagatt aggggctcag ccatcatata taattcaacg
     1021 ctctccaacc aatttcagcc ttcagccagc tcatttatcg tctttatcaa cccatcctct
     1081 cttcttttct ctggaaatag ttcaattatc cgttctctgg cttttttgcc cctattgctc
     1141 cgatccgcca gggctttttt gatggcctcc gccgcagctc gtggatcacc atagggcaca
     1201 ataaagccag tatctccaat tgctctggga atgccattgc gatctgtgcc cacaggaatg
     1261 cactcgcaga gcatcgcctc gcacagagca ttgggcagcc cttccctcat ggataattgg
     1321 cagaagaccc tggccctctg gtaggactca atgagatcat catgggtgac aaaccccgtg
     1381 aattccacat tatccggagc aatctccttt agccttgata aggcatcacc tttgatcccc
     1441 accaccttga agccagcctc cggaagatgt tttgccgatt ctacaaaaac atcgagtccc
     1501 tttatcagga cccggtccca gctctctccc atggcaacgg tcagcgccaa gggctccttt
     1561 tctcccttag gataaaactc tgtatctgaa aaacctgaag gaagatagtc tatattatgg
     1621 ccgttgatcc cggcattttt tatggcatcc tcctttaggg atggatcaac caccagcacc
     1681 ctgtccgcgt actttaaggc aaatttggtg agaatgctct tgatcctgcc ctgagcgaaa
     1741 cggccgtagt ttatctccgg gatgcatgca acgtcgaagc cgccaaccac gatgatggtc
     1801 ttttttctca gaagctttga aaggaggact gtggctgctg catggcctcc agcaaaccat
     1861 atcaaggcca tgcggcatct tatcatcgcc cctgccaatt tcactgcatc gccaagggaa
     1921 gataagctga ccacctccac ctcataatgg cgggacagca tatcgagatc tttttggatg
     1981 aaggaagaaa agtagccgta gtaaacaagg acgatcttca t
//
LOCUS       DAOSGP010000250         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_55862_length_2018_cov_12.825267, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000250 DAOSGP010000000
VERSION     DAOSGP010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55862_length_2018_cov_12.825267"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PLV49_06550"
     CDS             <1..935
                     /locus_tag="PLV49_06550"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOG98539.1"
                     /translation="HELIKKLGQSDDYFERQKAAWALVNLGDEAVDAVAEALEKGEFS
                     DLRYKSAWILGKTGSARALKPLCYALLSDPDHVVREWSAAAMEALGNQEAVQPLVLAM
                     KRDGSRDVRLRAAMALRALEAAEAFRDLLSYDDPEVRGMATTGLAKIGYAQSLPDVAR
                     LMTDESAEVRRRAAAYMGEIAREEGIEPLTRALQDDDVDVRCEALKSLGKIKGERAAD
                     LALAALPDKEWKVRYTAVTALGEIGHDKALDALIEVMFSQDDEEIRAWAAWSLGEIGD
                     KRAIEPLQKAYKTCPTEVMKKARDSLVEVFGEEV"
     gene            complement(957..1541)
                     /locus_tag="PLV49_06555"
     CDS             complement(957..1541)
                     /locus_tag="PLV49_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOG98540.1"
                     /translation="MTEGEKDPKEVAVLLLKATMLPDGEVFPRFDQPKYTPADYTRMA
                     CHILFRCQRCGTCCTTGDPIRLRPEDAAQLAKHLKIPLNRALKKYTIPDPKKPGVLDF
                     KHILPCKFYDRLAGGCKIYAARPWSCRIFPFLGVYGSSDQVVVNPSCPGSVETMKILT
                     RAMEEVQREPEGYSPSTEEIRRSKEHLRRVLDEL"
     gene            1599..>2018
                     /gene="engB"
                     /locus_tag="PLV49_06560"
     CDS             1599..>2018
                     /gene="engB"
                     /locus_tag="PLV49_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein EngB"
                     /protein_id="HOG98541.1"
                     /translation="MSKEVVLIGRSNVGKSTLFWELTGKKVPIGKRPGITQYPFKTKV
                     GNIFYVDMPGYGFMFRTTKADQEKTKDLIVHYFEEHAREILLAVQVIDAASFLDIADR
                     WEGRGEVPMEIELWEFLDDLGLDSVLAANKMDRIAKQD"
BASE COUNT          450 a          517 c          615 g          436 t
ORIGIN      
        1 ctcatgaact cataaagaag ctcggccaga gcgatgacta ttttgaacgt cagaaagcgg
       61 catgggccct ggtaaacctg ggtgacgagg cggttgatgc cgttgctgaa gccctggaga
      121 agggcgagtt ctcagacctg cgctacaagt ccgcctggat cctgggcaag acaggatctg
      181 ccagggcgtt aaagcctctc tgttatgccc tgctcagtga tccggatcat gtggttcgcg
      241 agtggagcgc agccgctatg gaggcactgg gcaatcagga ggcagtacag cctctggttt
      301 tggccatgaa aagagacggc tccagggatg tacgcttgag ggcggcgatg gccctccgcg
      361 ctttagaggc agcagaggct ttcagagatc ttctgagcta tgacgatccc gaggtccggg
      421 ggatggccac caccggcctc gcgaagatcg gctatgctca atccctgccg gatgtggccc
      481 ggctgatgac agatgagagt gcggaggtta ggcgaagggc ggcagcctac atgggagaga
      541 tagcccgcga agagggcatt gaacccctca ctcgtgccct gcaagatgat gatgtggatg
      601 tgcgctgtga ggccttaaag tccctgggaa agataaaagg ggagagagcc gcagatctag
      661 ctcttgccgc tctgccggac aaagaatgga aggtgcgcta tactgccgtg actgctctgg
      721 gggagatagg ccacgacaaa gcccttgatg ctctgataga ggtcatgttc agccaggacg
      781 acgaggagat aagggcctgg gccgcctgga gcctgggtga aatcggggac aagagggcga
      841 tagagccgct gcaaaaggct tacaagacct gccccactga ggtgatgaag aaggctcggg
      901 attctctggt ggaggtgttc ggagaagagg tctagctccg acttcaccat tgttgttcag
      961 agctcatcca gaactctcct cagatgctct ttgctccgcc tgatctcctc tgtgctgggg
     1021 gaatatccct ccggctccct ttgtacctct tccatcgccc gggttaaaat cttcatagtc
     1081 tccactgacc ccggacagga cggatttact actacctgat cggaagagcc gtagacccca
     1141 aggaatggaa atatgcggca ggaccaggga cgggcagcat agatcttgca gccgcccgcc
     1201 aatcggtcat agaacttgca gggcaggatg tgcttgaagt ccagaactcc gggctttttg
     1261 ggatcgggaa tagtgtactt tttcagggcc ctgttcaggg ggattttgag atgctttgcc
     1321 agttgtgcag catcctcggg ccgcaggcgg atggggtctc cggtggtgca gcatgtcccg
     1381 caccgctgac accggaaaag aatgtggcag gccatgcggg tataatctgc cggggtgtac
     1441 ttcggctgat cgaaccgcgg gaagacctct ccgtcgggga gcatcgtagc cttcaagagc
     1501 agaactgcga cctcttttgg atccttttcc ccttccgtca tttatgctat tgttctcaga
     1561 taggcatcct tttcaatctt gcccttcctc ttaatgccat gtccaaggaa gttgtcctca
     1621 tcggcaggtc caacgtcggc aaatccacct tattctggga gctcacggga aagaaggttc
     1681 cgatcggcaa gcgtcctggt atcacccagt acccgttcaa gaccaaggta ggcaacatct
     1741 tctatgtgga tatgcccggc tatggcttta tgtttagaac caccaaagcc gaccaggaga
     1801 agaccaaaga tctgatcgtc cattattttg aagagcatgc cagggagatt ctcctggcag
     1861 tgcaggtaat tgacgccgca tctttcctgg acatagccga ccgctgggag ggccggggcg
     1921 aggtgccgat ggagatagag ctttgggagt ttctggacga tttaggccta gactcggtcc
     1981 tggcagcgaa caagatggac cgaatagcca agcaggac
//
LOCUS       DAOSGP010000251         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_56062_length_2013_cov_20.044433, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000251 DAOSGP010000000
VERSION     DAOSGP010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56062_length_2013_cov_20.044433"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1321
                     /locus_tag="PLV49_06565"
     CDS             29..1321
                     /locus_tag="PLV49_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG98542.1"
                     /translation="MGSENDLDKCGAIEVAELLLTADIYNRKENLTEDDLPPNIRKHY
                     FDANTKSVKRPIYVTSSDVQKIYGIDNVKAAIKDLTFVSFEEFGSQLRLTVFDVAAKW
                     FANQKDLARIRSNPALAYFYENYDSMGVSYSEARNGNKPLSADREWIDARIKELCKDQ
                     KEADELLKLAYIKAPEDVRDSISGLILTDDQKTEIEKTGKALENREYLRQIGLFEIGK
                     LLFVGPPGTGKTSTARALSSWFSLPIVEVRLSMITSQYLGETSKNIDKVFELAKRLSP
                     CILLIDEFDFVAKTRTSDEHGAMKRAVNTLLKAIDEINLVEHGVLLLAATNHPQLLDY
                     AAWRRFDKVLNFPLPDQETRKRILDKILEPINANVDTTTLAKMTDGYSGSDLRLVIRE
                     AVLNALLENRKGLCQQDLLNSIDDFRQRISDYKRSIEA"
     gene            1318..>2013
                     /locus_tag="PLV49_06570"
     CDS             1318..>2013
                     /locus_tag="PLV49_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG98543.1"
                     /translation="MKVTLLGTGDAIGTPKIGCKCPACMDALRGGRSRRMRFSILLES
                     DEEDGRVLVDSSPDLRWQLLKKDIASVDGVIWTHAHYDHYAGFGDFHRVQSHVDVYAL
                     KNTMDYILNYLYFLAPVRHDVIAGQPFEIAGMQFTLFNVNHPPIETVGVRIDNGSKVV
                     VITSDTKMELPEESLELMRDADLMLADAITPPGYTISKHMTADEAMGLAERLGTKRLI
                     LTHLSHLYPPHEEA"
BASE COUNT          523 a          529 c          563 g          398 t
ORIGIN      
        1 tttagttcag ttccggggag ttaaatagat gggctcagag aatgatctag ataagtgcgg
       61 agcaattgag gttgcagagc tgcttttaac cgcagacata tacaaccgca aggagaacct
      121 gactgaggac gacctgccac ccaatattcg caagcattat ttcgatgcga ataccaagag
      181 cgtaaagcgg ccgatctacg taaccagcag cgatgttcaa aagatctacg gcatagataa
      241 cgtcaaagca gccatcaagg atcttacttt tgtttccttc gaggagttcg ggtcgcagct
      301 gaggctcacg gtattcgatg tggccgcgaa gtggtttgcc aatcagaagg atcttgccag
      361 gatcagatcc aatcccgctc ttgcctactt ttatgagaac tatgactcca tgggggtaag
      421 ctactccgag gccagaaacg gtaacaagcc actttctgca gaccgcgagt ggatcgacgc
      481 ccgcataaaa gagctgtgca aggaccaaaa ggaggcagat gagcttctca agctggctta
      541 catcaaagcg ccagaggatg taagagacag catctccggt ttgatcctga ccgatgatca
      601 gaagacggag atcgaaaaga cgggaaaggc gctggagaac agagaatacc tgcgccagat
      661 aggcctgttc gagatcggca agctactatt cgtcggcccg ccgggaacgg gaaaaacctc
      721 cactgcccgg gcgctctcca gctggttttc tctcccaatt gtagaggtcc gcctctccat
      781 gatcaccagc cagtacctgg gggagacatc caagaacata gacaaggtat tcgagcttgc
      841 caagaggctc agcccctgca tccttctcat cgatgagttc gatttcgtgg ccaagaccag
      901 aacctcagac gagcacggtg ccatgaaaag agcggtaaac actctgctaa aggccataga
      961 cgagattaac ctggtcgagc acggcgtcct ccttcttgcg gccacaaacc acccccagct
     1021 gctggattat gccgcctgga gaaggtttga caaggtgcta aacttccccc tgcccgacca
     1081 ggagacgaga aagaggatct tagacaagat actggagccc atcaatgcca atgtggatac
     1141 cacaacgctg gcaaagatga ccgatggcta ctcgggttcc gatctgaggc ttgtgatcag
     1201 agaggcggtg ctcaatgcac tactggagaa cagaaagggg ctatgccagc aggacctcct
     1261 gaactccata gatgacttcc gccagaggat atcggactac aaacggagca ttgaggcatg
     1321 aaggtcaccc tcctgggcac aggcgacgcc attggcactc ccaagatcgg ctgcaagtgt
     1381 ccagcctgca tggatgccct ccggggaggg cgcagccgaa ggatgaggtt ttcaatcctg
     1441 ctggagtcgg acgaggagga cggccgggtg ctggtggact cgagccctga cctgcgatgg
     1501 cagctcttga aaaaggatat cgcgagtgtg gacggggtga tctggaccca tgcccattat
     1561 gatcattatg cgggcttcgg agacttccat cgggtgcaga gccacgtgga cgtctatgct
     1621 ctgaagaata ctatggacta catactcaat tacctctact tcctggcccc agtgcgtcat
     1681 gatgtcatag ccggccagcc cttcgagata gcaggaatgc agttcaccct cttcaatgtc
     1741 aaccaccccc ccattgagac cgtgggggtg cggatagaca atggaagcaa ggtggtggtc
     1801 attaccagtg acaccaagat ggagctccct gaagagagcc tggagctgat gagggacgct
     1861 gacctgatgc tggctgatgc cataaccccg ccgggctata ctattagcaa gcacatgacc
     1921 gcagacgaag ccatggggct ggcggagagg ctgggaacga agaggctgat cctcacccat
     1981 ctgagccatc tgtatccgcc ccatgaggag gct
//
LOCUS       DAOSGP010000252         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_56134_length_2011_cov_17.856339, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000252 DAOSGP010000000
VERSION     DAOSGP010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56134_length_2011_cov_17.856339"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..363)
                     /locus_tag="PLV49_06575"
     CDS             complement(121..363)
                     /locus_tag="PLV49_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98544.1"
                     /translation="MLLALAYQLSGIDILDPGRSYIAPEQNIIALCGHYMSPALDRYL
                     AAAFDGELRRFAGIGIFSEIPGVSFYLYWILFCIGD"
     gene            784..1971
                     /locus_tag="PLV49_06580"
     CDS             784..1971
                     /locus_tag="PLV49_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98545.1"
                     /translation="MFAENAYDTGVRMGTIDLGSTVLTGNTQLNFTRILDILIDFAEF
                     YNLNPRFRRGKDYTYFDALDDPVESEFVLPEENIAKITQQYNEDSKVHALIGKGTGSE
                     DVQQIYAPSDHTWKGIWIADTMDIEDGYLDSLGIAKPFVNAEYSYRQADEKFSITPTP
                     DWGHMPRPFSMIRLKLDGEAEKLLQVMSARRDEAGNYDMEIGGRDSDILDAFNGRDSL
                     DRVYLEDYLTEYGEAISLDGEDFVLGDTTHGTCTGASGTVLIPAAVYESDWSHRVTLD
                     ISTKTNASPVSCMMIIEINGASNFLCQPRHYLLGETISGLDITRYCNYGTNTAISVWI
                     QKNGEWTGANCAAHPKFDLSITVRCWKRTIPGSTVKRGMNKKKIIFADWKKMKKKVTR
                     WMK"
BASE COUNT          556 a          461 c          533 g          461 t
ORIGIN      
        1 atggcaaagc atgagcacaa agccgacaaa ggcgagccct ggagcaaaaa gaaagaagaa
       61 gatgagcaga gcgggaagcc tcagaaaaag tatcttgttg gagtctaata aatggaaatc
      121 ctagtcacca atgcagaaaa ggatccaata gagatagaag ctgactcctg ggatctccga
      181 gaatatgccg atgccagcaa agcggcggag ttctccatca aatgcagccg caagatacct
      241 atccagcgct gggctcatgt agtggccaca gagggcaatg atgttttgtt caggggctat
      301 gtaacttcgc ccaggatcaa gaatatcaat accagagagc tggtatgcaa gggcgaggag
      361 catctcttgc tcaggcgatt taccggcaga ttctcttatc aggcctccgc ccgctatttg
      421 gctcatgtct tccagagcga tgcacctagc cagacggcag acagctatgg cgtcaccgga
      481 aacgtgggaa tgctctttat ggccaattcc ctcattccat attacggcaa cgtcctgacc
      541 actgggacac ctgcctatga ctggtgggca gagggggcgg actggatcta taagctctcc
      601 gggctgggca cggccagcag gataggcact gccaatatct atgcagaggg caaactcctg
      661 ccccgccaga gcacctatgc ggctctccag gccaccgcta tatcctgcta ttctgacgcc
      721 tctgatttat gggtccggct agataatgca agttataacg tgggattcgg tccgcggtat
      781 tgcatgtttg cagagaatgc ctatgacact ggcgttagga tgggcaccat cgatctaggg
      841 agcaccgttt taactggcaa tactcagctt aactttacca ggattttaga tatcctgata
      901 gattttgctg agttctataa tctaaatccg agattccgcc gtggcaagga ctacacctat
      961 tttgatgccc tggatgatcc ggtggaaagt gagtttgtcc ttccggagga gaacatagcc
     1021 aaaataacac aacagtataa tgaggatagt aaagttcatg ccctcatcgg aaaagggaca
     1081 ggctccgagg atgtccagca gatctatgca ccctcagatc atacatggaa agggatctgg
     1141 attgcggata ctatggacat cgaggacggc tatctggatt ctctgggaat agcaaaacct
     1201 ttcgttaatg cagaatacag ctaccggcaa gcagatgaaa aattttcgat aacaccaact
     1261 ccagactggg ggcacatgcc caggccattc tcgatgattc ggttaaagct ggatggcgag
     1321 gcggaaaaac tcctgcaggt gatgtctgcc cggagagacg aggcaggcaa ctacgatatg
     1381 gagattgggg gccgtgactc tgatatcctg gatgctttca atggcaggga ctcactggat
     1441 cgggtctatc tggaggatta cctgaccgaa tacggcgagg ccatttccct ggatggtgag
     1501 gattttgttc tgggggatac tactcatggg acatgcacag gagcatctgg caccgtgctt
     1561 attcctgccg ctgtttatga atctgactgg agccaccggg taactttgga tatctccacc
     1621 aaaaccaacg cgtccccagt gtcttgcatg atgataattg aaatcaatgg cgcctcaaat
     1681 ttcctttgtc agcccaggca ttacctcctc ggagagacca ttagcgggct cgatatcacc
     1741 cgatactgca attatgggac aaacactgcg atttcggtat ggattcagaa aaatggagaa
     1801 tggactgggg caaattgcgc cgctcatcct aaatttgatc tgtcaatcac ggttcgatgt
     1861 tggaagagaa cgataccagg aagcacagtt aagcgcggca tgaacaaaaa gaaaataatt
     1921 tttgctgact ggaagaagat gaagaaaaag gtcacaaggt ggatgaagta atggccgaag
     1981 tctcaaaata ttgtgctgtt tgggattatg a
//
LOCUS       DAOSGP010000253         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_56136_length_2011_cov_13.623722, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000253 DAOSGP010000000
VERSION     DAOSGP010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56136_length_2011_cov_13.623722"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLV49_06585"
     CDS             <1..362
                     /locus_tag="PLV49_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG98546.1"
                     /translation="LSFLKSLKGIGEIVLLNVVSKGETDEEIEANVALAKGRIEEIAQ
                     NLKMSGIQATTKVVVGQPAEVIRSEAEKEDVSLIAISSQGASAIKKGRIGSTAYDVAN
                     SASRPVLILRQEKMAAY"
     gene            803..1939
                     /locus_tag="PLV49_06590"
     CDS             803..1939
                     /locus_tag="PLV49_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HOG98547.1"
                     /translation="MQFDPLDIGLLIIVLAVLVGPFMVKKIEHNLEAFLFVMGVLAVT
                     ISSFYSKPGLMEMLGYTEHQVELIGWNMEIVMEAIKEPVIKGIVPAVLVAGILFHYGR
                     SYAQMAMSKITKVVPIKIVVFLIVVVLGLSSSIITAIIAALLLVELLNCMPLDRKTKI
                     NVVIIACFSIGLGAVLTPVGEPLSTIAITKLQGEPYNAGFFFLFNQLAVYIIPGCLAF
                     GLLAMFFTGKAKLADCMVAEEEDGGLREVVIRAGKVYIFVMALLLLGSGMKVVIDKYM
                     LGIAPQILYWVNMISAILDNATLTAAELSPAMEMTQIQAVLMGLLISGGMLIPGNIPN
                     IISAQKLNITSKEWAKLGVPLGLAVMLVYYVWIFYIPFHIEFAL"
BASE COUNT          508 a          482 c          514 g          507 t
ORIGIN      
        1 ccctgtcctt cctgaaaagc ctgaagggaa ttggagagat agtgctgctc aatgtggtat
       61 ccaaggggga gaccgatgag gagatcgagg ctaatgtcgc attggccaaa gggaggatcg
      121 aggagatcgc ccagaatctg aagatgtcgg gcatacaagc cacaaccaag gtggttgtgg
      181 gccagccggc tgaggttatc agatcggagg ctgagaaaga ggacgtctcc ttgattgcca
      241 tcagctctca gggagcctcg gccataaaga agggcagaat aggaagcact gcttatgacg
      301 tggccaactc cgcttccagg ccggtcctaa tattgaggca ggagaagatg gctgcatatt
      361 agttcctttt ctttttagct tttaatattt ttaaaaacca gttgctcgat agcatgatgt
      421 acactgcagc cagacgccga gatttattgt atcatttgcc acaattcttc tagttctaga
      481 tgcctctttt ttcaattatc taacttgatc caggcattgg ctcgggtatc aaatcggttt
      541 gagcctccaa aacaaaaaac caaaagcatt taatattggg ttgagcatcc caataagaga
      601 ctctccggat gagggagcct gtcgatgcag gaatgaatgg agtggtcata tggtttcaac
      661 atatagagag cttgattgga gttgggggat acggaactat ctggccgtac ggccaaaacc
      721 atgagaccca atgtcagcct ctggcctgca gagacggaaa ctaacgatat tgaaaatttt
      781 gtagatattg gagggaatat ttgtgcaatt tgacccacta gacattggtc tacttatcat
      841 tgtactagct gtacttgtag gaccattcat ggtcaagaag atcgaacaca accttgaggc
      901 ctttctattc gtcatgggcg ttctggcagt gaccatatca agcttttaca gcaagcctgg
      961 acttatggag atgctgggat acacagagca tcaggtagag ctcatcggct ggaatatgga
     1021 gatagtgatg gaggccatca aagagccggt gataaaagga atcgttcccg ctgttctcgt
     1081 agccggcata ctcttccact atggccgctc ctatgcccag atggcgatga gcaagatcac
     1141 caaggttgtc cccataaaga tcgttgtatt tctgatcgtg gtcgttttag gactatcctc
     1201 cagcataata accgcgatca tagccgcgct cctcctggtg gagctattga actgcatgcc
     1261 ccttgaccgc aagaccaaga tcaatgtggt catcatcgcc tgcttctcca tcggcctggg
     1321 agcggttctc acaccagtgg gtgagcctct ttccaccatc gccatcacca agctgcaggg
     1381 tgagccctac aatgcaggat tcttcttcct cttcaaccag ctggcggtat acatcatacc
     1441 cggctgcctg gccttcggcc ttctggccat gttcttcact ggcaaagcca agctcgctga
     1501 ttgcatggtc gcggaggagg aggacggcgg cttaagagag gtagtgatca gggcaggcaa
     1561 ggtctacata ttcgtcatgg ccctcctgct tctgggctca ggaatgaagg ttgtgataga
     1621 caaatatatg ctgggcatag ctccccagat cctctactgg gtgaacatga tctcggccat
     1681 attggataat gccactctga ctgcggctga acttagcccg gccatggaga tgacccagat
     1741 tcaggccgtt ctcatggggc ttctcatctc tgggggcatg ctgatcccag gaaacatccc
     1801 caacatcatc tccgcccaga agctgaatat caccagcaag gagtgggcaa agcttggcgt
     1861 tcccctggga ctggcagtta tgctggtcta ctatgtgtgg atattctaca tacctttcca
     1921 cattgagttc gccctttaga agtgatgctc gcatcacttc ttttcacatg aaaaacataa
     1981 gaaaatcagg atgggatggt catccacatc c
//
LOCUS       DAOSGP010000254         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_56179_length_2010_cov_22.554476, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000254 DAOSGP010000000
VERSION     DAOSGP010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56179_length_2010_cov_22.554476"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2010)
                     /gene="fdhF"
                     /locus_tag="PLV49_06595"
     CDS             complement(<1..>2010)
                     /gene="fdhF"
                     /locus_tag="PLV49_06595"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOG98548.1"
                     /translation="LCPYCAVGCGLFIQVEKGKATGIKYMTDHPTCVGALCPKGNSVL
                     ELLNNQERLVYPLKRSGSDFIPISWDEALELVAQGLARNLERYGPKSLGFLVSSRCNN
                     EENYLMQKMARLLGSPNVDNCARLCHSPTVMGLGASFGAGAMTNNLLDLENSKCIFAI
                     GTNFTEAHPIVSRWVEKAKDNGAVVIVADPRITSTTWMADIHLRLNPGSDIDLLRGMM
                     KVIVDEGLEDADFISSRTTGFQELKESLSGFSMEEAAEKTGLDVAEIVKAARVYAKSS
                     ASALLYSMGITQHVSGTDNVMTCATIALICGQIGRPGAGVWPMRGQNNVQGNCDVGGM
                     PEFYPGYRRSVDPKTIEFFRAAWNATDLPLGPGLTSTDMNDAAIDGCLKALYITGEDP
                     IVCDANVNKTRRALESLDFLVVQEIFMTPTAKMADVILPAAAWAEKSGSYTSMERRVQ
                     WIDKAIEPPGEAKEGVWIICEIARRLGLGEGMAYDNPSQVLEEIRSLVPQYKGMSQER
                     ISRVGGVLWPCPDEQHPGTEILHLQRFSTSDGRARIFDLQNTPPGEVTSPEYPILLST
                     GRIVLHYNGGSMTMRSPSLQERESELYVEVNPLDASNLKIAEGEMVAVKTRRGEAEAR
                     AKISDRVMSGMAFMPFHYHGTNLLTSDALDPVSRIPEYKMAACRIE"
BASE COUNT          390 a          597 c          567 g          456 t
ORIGIN      
        1 ctcaattctg caggcggcca tcttgtattc cgggatcctg gatacgggat caagggcgtc
       61 ggatgtcaag aggttggtcc cgtgataatg gaagggcatg aaggccatcc ccgacatgac
      121 cctatccgaa atttttgccc gggcctccgc ctctcccctg cgggttttca ctgccaccat
      181 ttcgccctca gcgatcttga gatttgaggc atcaagcggg ttgacctcca catatagctc
      241 tgactcccgc tcctgcagtg acgggctgcg catggtcatc gagccgccat tgtaatgaag
      301 cactatcctt cccgtgctga gcagaatggg atactccggg ctggtgacct ctccgggagg
      361 agtgttttgc aggtcgaaga tcctcgccct gccgtctgag gtggagaacc tctgcaggtg
      421 cagtatctcc gttccgggat gctgctcatc tgggcagggc cagagaaccc caccgaccct
      481 cgatatcctt tcctggctca tgcccttgta ttggggcacc aggctgcgga tctcctctaa
      541 cacctgggag ggattatcat aagccatccc ctcgcccagt cccagccgcc tggcgatctc
      601 gcagatgatc cagactccct cttttgcctc ccccggaggc tctatggcct tgtcgatcca
      661 ttgcactctt ctctccatgg aggtatagct gccgctcttc tccgcccagg cggcagcagg
      721 caggatgacg tccgccatct tcgctgtggg agtcatgaat atctcctgca ccaccaggaa
      781 atccaggctc tccagagcgc ggcgggtctt attgacattg gcgtcgcata ctatcggatc
      841 ctctccggtg atgtaaaggg ccttgaggca tccgtctatt gctgcatcgt tcatgtcggt
      901 cgaggtcagg cctgggccga ggggcaggtc agtggcgttc caggccgccc ggaaaaactc
      961 aatcgtcttg ggatccaccg atctcctgta gccaggatag aattccggca ttccccccac
     1021 atcgcagttc ccctggacat tgttctggcc gcgcatgggc cagactccgg ctccaggcct
     1081 tcctatctgg ccgcagatga gggcaatggt ggcgcaggtc ataacgttat cagttccaga
     1141 gacgtgctgg gtgatgccca tggaatagag tagagctgag gcggaagact ttgcatagac
     1201 cctggctgcc tttacgatct cggcaacgtc caggcccgtc ttctctgccg cctcttccat
     1261 tgagaaaccc gacaggctct ctttgagctc ttggaagccg gtggtgcggg agctaataaa
     1321 atcagcatcc tccagacctt catcgacgat gaccttcatc atcccccgga gaaggtctat
     1381 gtccgatcca ggattcaggc gaaggtgaat gtccgccatc caggtggtgg aggtgatccg
     1441 gggatcggcc acaatcacca ctgccccatt atcctttgcc ttctctaccc agcgggagac
     1501 gattggatgg gcctcagtga agttggtgcc gatggcgaaa atgcatttgg agttctccag
     1561 gtcgagaagg ttgttggtca tggcaccggc gccaaaggag gcgcccagac ccatcaccgt
     1621 gggtgaatgg cagagcctgg cgcagttgtc cacgttgggt gatcccagga gccgggccat
     1681 cttctgcatc aggtagttct cctcgttatt gcagcgggag gagacgagaa agcccaggga
     1741 cttcgggcca taccgttcca ggttccttgc cagaccctga gccaccagct ccagggcctc
     1801 gtcccagctt atgggaatga aatcgcttcc agaccttttc aggggataca cgagacgttc
     1861 ctggttattc aaaagctcca gaacggagtt ccccttgggg cagagagcgc caacgcaagt
     1921 tgggtggtcg gtcatgtatt tgatgcctgt ggctttgccc ttctcgacct ggatgaatag
     1981 gccgcaaccc acagcacaat aggggcacag
//
LOCUS       DAOSGP010000255         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_56221_length_2009_cov_10.537359, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000255 DAOSGP010000000
VERSION     DAOSGP010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56221_length_2009_cov_10.537359"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1003)
                     /locus_tag="PLV49_06600"
     CDS             complement(98..1003)
                     /locus_tag="PLV49_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOG98549.1"
                     /translation="MDWFAFSIAGAACLAVTGIIDKFYLGRYVRNILAYLFVLVVIQQ
                     VFLLPVLAYAGLDFVYPQSLYALSIGVVQVILWAAYLRALKVEETSRIAGQVYVFPVF
                     VFLGEFFLLGETLQPSDYAGGTLLVFAALLISYRPASHPGRIAGGGRISPALKYMAVF
                     WVFTAAYSLASKYLLDYMTEWHLIVWSSLGSLVAVFALLARGEVRSEVGGYFRSGPRL
                     ISILFLNELFDFLGRGSLIFAYALGSVALVSSVSALQPFITLIYVLVLGIFLPGMLVE
                     DSDGRTVALKIGATMLIVAGVYLVS"
     gene            complement(1058..>2009)
                     /locus_tag="PLV49_06605"
     CDS             complement(1058..>2009)
                     /locus_tag="PLV49_06605"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOG98550.1"
                     /translation="LIYGAGAIGSLMGYLLSDPQSSNSSMVENVALLGRSRHMEKIRS
                     EGLEVEAPDGTTLVHFRHLFTDLDDLLRSDFHPDVVMICVKTYSLQKLCQELGQSGIL
                     EDRLKNASFLLLMNGMGNRELFETQVPGALGRAFEGITSNGVKLAREGKIELKGKGTT
                     VIEKSLPQELEQFIKSRFEDRGFVVEFSADFKRQQWNKLFINSVINPIAALARQQNKV
                     ILSPVLQSTVKRVVGEGVAVAGAEGFGFDPDTVLELVLSVAEKTGENSCSMLQDVLNN
                     KTTEIDSINGYIVRLGELHSIAVPVNEALYGLIKATARGS"
BASE COUNT          480 a          589 c          528 g          412 t
ORIGIN      
        1 ctattggggc atcaaatatg gatcgctgcc atattccctt ttccagtttt cactacgcca
       61 gggttcctcc tcactcaata cagtgagatc aggccgatca ggacaccaaa tacaccccgg
      121 caacgatcag catcgtcgcc ccgatcttca gggcaacggt tctgccgtcc gagtcttcca
      181 ccagcatccc gggcaggaat atgcccagaa ccagcacata aatcagagtg ataaagggct
      241 gcagagcgga gacgctggag accagggcca ctgaccccag ggcataggca aatatgagcg
      301 atcccctccc caggaagtcg aacagctcat tcaagaaaag aatcgagatc agcctcggac
      361 cggatcggaa atagcccccc acctcgcttc gaacctctcc tctggccagg agggcgaaga
      421 ctgcgaccag gctgccgagt gacgaccaga cgatcagatg ccactcggtc atgtagtcga
      481 gcaggtactt agaggccagg gagtaagcgg cagtgaagac ccagaagact gccatgtact
      541 tcagagcagg tgagatcctg cctcctccgg cgattcttcc aggatgggag gccgggcggt
      601 aggagatgag cagagcagcg aagaccagca gggtaccgcc cgcatagtca gagggctgga
      661 gggtctcgcc taaaaggaag aactccccca ggaagacgaa taccggaaat acgtagacct
      721 ggccggcaat cctgctcgtc tcctccacct tgagggcgcg caggtacgcc gcccagagga
      781 tcacctgcac cactccgatg gacagggcat agaggctctg aggatagacg aaatccaggc
      841 cggcataggc taggactggc aggaggaata cctgctgaat aaccaccagg acgaatagat
      901 atgccaggat attgcggaca taccttccca ggtagaactt gtctatgatc cccgtgaccg
      961 ccaggcaggc ggcgcccgcg atagagaatg caaaccagtc cacgaaatat cttctcgcca
     1021 ggaggtgtta tagatttccc atactgaaaa tcaattctta tgagcccctg gcggtcgctt
     1081 tgatcaggcc gtacaatgcc tcgttcacgg ggacagcaat ggaatgcaac tcgcccaggc
     1141 ggacgatata gccgtttatg gaatcgatct ctgtggtctt gttattcagg acatcctgga
     1201 gcatgctgca gctgttctct cccgtcttct cggccacgga gaggacaagc tcaagaaccg
     1261 tatctggatc aaagccaaac ccctccgctc cagctaccgc cactccttct ccgaccaccc
     1321 ttttcacagt gctctgcaaa accggggaga ggatgacttt attctgctgc cgggccaggg
     1381 cggcgatggg gtttatcacc gagttgatga agagcttatt ccattgctgc ctcttgaaat
     1441 ctgctgagaa ctcgaccacg aatccccggt cctcgaaccg ggactttatg aactgctcca
     1501 attcctgtgg gaggctcttt tcgataacag tcgtgccctt gcccttcagc tcgattttgc
     1561 cctcccgggc gagcttgacc ccgttggatg tgatcccctc aaaagccctt cctagggctc
     1621 cagggacctg cgtctcgaat agctccctgt ttcccatgcc gttcatcagc aggaggaagc
     1681 tggcgttctt cagtctgtcc tccaggatgc ctgattgtcc gagctcctgg caaagcttct
     1741 gcagggaata ggtcttgacg catatcatca ccacatccgg atgaaaatca gatctgagaa
     1801 gatcatcaag gtcggtgaac agatgcctaa aatgaaccag cgtcgtgcca tcgggagctt
     1861 ctacctccag gccctcggat ctgatctttt ccatatgccg ggatcttccc agcaaggcca
     1921 cattctccac catgctgctg ttcgacgatt ggggatcgga gagcagataa cccatgaggg
     1981 agcctatcgc tcctgctccg tatatcagg
//
LOCUS       DAOSGP010000256         2008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_56253_length_2008_cov_15.891449, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000256 DAOSGP010000000
VERSION     DAOSGP010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56253_length_2008_cov_15.891449"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLV49_06610"
     CDS             complement(<1..475)
                     /locus_tag="PLV49_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HOG98551.1"
                     /translation="MRAINAVLLLALLLIFSGCVERPEAPAVSEQEATEEWKADGIVS
                     EGEYTRSMLLQAPTRQGYSGGDMQISWRNDEEDLYLALNGSTLGWLALGFEPLEWMKD
                     SDIILASVDKGTAVVLDEYCTGNYGPHIEDTMLGGTDDIQEFSGSESAGRTTIELK"
     gene            complement(787..1371)
                     /locus_tag="PLV49_06615"
     CDS             complement(787..1371)
                     /locus_tag="PLV49_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98552.1"
                     /translation="MKDGEALKLKKPVIEACIKSARKYGKSDAIGLLRGTLFLESEPM
                     SLDMLAESTGYSKTTVRSSMNLLENLGIARRVVDPQDMHHNIKQHRYALVNDAEAKRQ
                     VILSAAKEEVDLILQALLQVRNNLEREEDEGCNRARDKGYKPEKDEGYRRDDKLKALL
                     AESLQFYEEMKRTLDLISRFTSKELIEILEEHDR"
     gene            1463..1912
                     /locus_tag="PLV49_06620"
     CDS             1463..1912
                     /locus_tag="PLV49_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG98553.1"
                     /translation="MKSYPSGSSDLEQMIGNAAVEVARISIQAAKKILGDLPSDISME
                     KSQDQPAIDLMDGKDELVALVSLPGRSKDMIDLRVTEDTITISAKSAAREGKYLRQEI
                     ENRNVEREIKLPLEVKPEQVKASFNNGILEVHLPKLMVVDSQRIQVE"
BASE COUNT          466 a          538 c          480 g          524 t
ORIGIN      
        1 tcttcagctc tatggtggtc ctgccagctg attcgcttcc agaaaactcc tggatgtcgt
       61 ctgtgccacc caacatggta tcctcgatat gcgggccgta gttgcctgtg cagtactcat
      121 ctaatactac agcagtgcct ttgtccaccg aggccaggat tatatcggag tccttcatcc
      181 actccagagg ctcaaagccc agcgccagcc agccaagagt gctgccattc agggctagat
      241 agagatcttc ttcgtcattt ctccaggaga tctgcatgtc tccgccgctg tagccctgcc
      301 tggtcggggc ctgaaggagc atgctgcggg tgtattcccc ctcgctgact atcccatcgg
      361 ccttccattc ctctgtggcc tcctgctctg agaccgccgg ggcctctggc cgctcaacgc
      421 agcctgaaaa gatcagcagc agagccagga gcaaaacagc attgatcgct ctcatatcca
      481 aacaccattt caagatattt gggctgcaac ctatatcagg ctatgccatt tgcagcttat
      541 tttctccaat ctctcctcga gcttcgatag aggaaaactt agatatacca aaacatcact
      601 attaatgatt gtgattgatc atgataaggc tcgtcattgc attccttctc ttgtggctgc
      661 tggcggcttg gattcttgcc tggccaagaa gaacgaccag agcgtaaatc ctaaaggtca
      721 gcctattgag atgagatctt aatcgatgat agctggctag ctagggggtc gacatagaga
      781 tctcttttat ctatcatgtt cttccaggat ctctatcagt tcctttgagg tgaaccggct
      841 tatcagatcc agcgtccttt tcatctcctc ataaaactgc agggattccg ctaaaagggc
      901 tttcagctta tcgtctctcc tgtacccttc atccttctct ggcttgtacc ctttatccct
      961 tgctctatta caaccttcat cctcttctct ttccaggtta tttctcacct gaagaagggc
     1021 ttgcaggatc agatcaacct cctctttggc tgctgagaga atgacctgcc tttttgcctc
     1081 ggcatcgttc accagggcgt agcgatgctg cttgatgtta tgatgcatat cctgggggtc
     1141 gaccacccgg cgagctatcc ccaggttctc cagcaggttc atgctggagc ggacggtggt
     1201 tttgctgtag ccggtgctct ctgccagcat atccagggac atgggttcac tctccaggaa
     1261 gagggtgcct cgcagaagtc ctatggcatc actctttcca tatttccgag ctgatttgat
     1321 gcacgcctct attactggct ttttcagttt cagtgcttcc ccgtccttca cagctttcca
     1381 ctcgaaagag gcgtttaagt aacttgctct ccccgcggca aggaagcaaa ggattttatg
     1441 atacaaagac ctatttttgc acatgaaatc atatccatct ggaagcagcg atctggagca
     1501 gatgataggc aatgcagcgg ttgaggtcgc tcgcatatca attcaggcgg cgaagaagat
     1561 cctgggtgac ctgccaagcg atatctctat ggagaagagc caggatcagc ctgctataga
     1621 tctcatggac ggaaaggacg agctggtcgc tctcgtatcc ctgccgggaa ggagcaagga
     1681 tatgatcgat ctccgagtga cggaggacac catcaccata agcgccaaat cagcggcccg
     1741 cgagggaaaa tacctgcgtc aggagatcga gaaccgaaat gtagaacgtg agatcaagct
     1801 gcctcttgag gtcaagccgg agcaggttaa agccagcttc aataacggca tcctggaggt
     1861 ccatcttccc aagctaatgg tggtagactc ccagaggatt caggtggaat gatatcgaga
     1921 cccatttttt tcgctaaaaa aacaaaaaag tcaaggatga acgtcctctt gctgggcgtt
     1981 gtcagctttc tcaacgacat cagcagcg
//
LOCUS       DAOSGP010000257         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_56704_length_1998_cov_8.422542, whole genome shotgun sequence.
ACCESSION   DAOSGP010000257 DAOSGP010000000
VERSION     DAOSGP010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56704_length_1998_cov_8.422542"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..614
                     /locus_tag="PLV49_06625"
     CDS             300..614
                     /locus_tag="PLV49_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOG98554.1"
                     /translation="MGKVVGAKLTRNEILQINRLVDAGLYLNPSDFIRDAVREKLAAI
                     KVIEYRNADYETAKKEVAGYFQMKGEAYASDASTDLQLDYELVIKIMDDLEKEGKMET
                     VK"
     gene            611..793
                     /locus_tag="PLV49_06630"
     CDS             611..793
                     /locus_tag="PLV49_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98555.1"
                     /translation="MKGYDVGDSIHGHRIIAEARRVSDPMVIVKSARHRAQRIVLTEN
                     ALAIIDRKGRWCGQSP"
     gene            complement(801..971)
                     /locus_tag="PLV49_06635"
     CDS             complement(801..971)
                     /locus_tag="PLV49_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98556.1"
                     /translation="MFRGNYRLELSPGMHIIDPFKSGYGIPPRAAFVTAGQTTGLDFI
                     AKGVVALGRGRM"
     gene            complement(1241..1520)
                     /locus_tag="PLV49_06640"
                     /pseudo
     CDS             complement(1241..1520)
                     /locus_tag="PLV49_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611503.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          542 a          437 c          539 g          480 t
ORIGIN      
        1 tcacagtgaa agctcaatcg ggagagtctc tgtggtcagg atgcagcgga gtggggctgg
       61 agcgctgggc cagtgccttc ctgagccaga agggcttgga tccatccaac tggccggagg
      121 agctccagaa gcgtttcggg gagatgccca aggggatcag gttcctctga tcccattttc
      181 aatattttat tgggcttgaa atgattggat agtgctggca gcactcagac cgataagcct
      241 atgaattagt tttaactaat aaaataggta tgggagcgga tatcgccagt actggaaatg
      301 tgggaaaagt agttggggcc aagctaactc gaaatgagat tttgcagatc aaccggcttg
      361 tagatgccgg tctgtatctc aatccctcag atttcattcg cgatgctgtc cgggaaaagc
      421 ttgctgcaat caaggtcatt gaataccgca atgcggacta tgagactgct aaaaaggaag
      481 ttgctgggta tttccagatg aagggcgaag catatgcatc tgatgccagc accgacctgc
      541 agctggacta tgagctggta atcaagatca tggatgacct ggaaaaggaa ggcaagatgg
      601 agacagtaaa atgaaaggct atgatgtcgg cgactcgatt cacggtcaca ggatcatagc
      661 tgaggcgaga cgggtgagcg atcccatggt catagtgaag agtgccaggc acagggcgca
      721 gcgaatagtc ttgacagaaa atgccctggc aataatcgat agaaaaggga gatggtgcgg
      781 gcaaagcccg tgattttcaa tcacattctc cctctcccca gggctaccac ccctttggcg
      841 atgaaatcca ggccggtcgt ctgcccggcg gtgacgaatg ctgcccgggg cggaattcca
      901 tacccggact tgaatggatc gataatgtgc attcccggtg agagctccag gcggtaattt
      961 ccccgaaaca agagcgcgcc aagagaacga agatttcagc gaggcggccc acgtagatga
     1021 ggaatacgaa tatctgctta aaaacaagga tcaaggcata accttcgaag agtattgccg
     1081 cagacgcgga atcaatatct aagcgcgaga tcgtattctc agggcgtttg tgagcagata
     1141 ctttactgca tgtgggcgag aaagcgatct gcagagcagc catctatgta ttatcattgg
     1201 gatttctttt ctggacatgc ggatcataat gccatgtttt ttttaaacag tcgacataat
     1261 ctttagatgc atactgcact ccttgatcag gatgatggat gaggccggtc agggattctg
     1321 accatctatt cctcaatgcc ttcgctaatg catttggcca gttggtcact caaacttctg
     1381 cccagattcc agctgatgca ctttctgctg gagagatcca ggataactgc cagatagata
     1441 ttctcatgcc gcagctggat gtatgtgata tccaataccc ggatccgatt caatccggtt
     1501 actgttgtgc tcttcagaag gagtttggcg ggctcaaact cggctactac tgatcttgaa
     1561 ttcctggtcg cagagcagtc cggtcttttt tcatctgatc ccctctgtaa gttggcattt
     1621 cttaactggt tgtccacccg gaggggttca ctccaatgct gcatcgtaat tctgaagcag
     1681 aagcggcgta taagaaagca cgagaactgg gatacagcgg agaaatgaca ttgatggaga
     1741 tgacgacgaa ttaagagtat caaatctgtc cggcctgagg tgaataggag ggggaaagcc
     1801 gaaagggtaa aggagggaga aaaactctat cgactttcgc ctttgactat atagacaatt
     1861 gtcgatatat atagttttcg atccaggaaa caaatctgct cttaaaggac gctcgtcgcc
     1921 gaaaggctca tcatagatcg gcctgcaaaa ataagttgtg ctccatatgg ccgcgtataa
     1981 caagatgcct ctggaaac
//
LOCUS       DAOSGP010000258         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_57375_length_1983_cov_15.086618, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000258 DAOSGP010000000
VERSION     DAOSGP010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57375_length_1983_cov_15.086618"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLV49_06645"
     CDS             complement(<1..409)
                     /locus_tag="PLV49_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HOG98557.1"
                     /translation="MLKMSPDDKLTDLKDRIREKGRLLISYSGGVDSSLLAKLAHDVL
                     GEGALAVILDSEAYSRSELNEAVALAESLGLRLRVERFSILEDEAFSQNPATRCYICK
                     KRSSAILKRVAQKEGISCIADGVNADDLDDYRPG"
     gene            complement(515..841)
                     /locus_tag="PLV49_06650"
     CDS             complement(515..841)
                     /locus_tag="PLV49_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG98558.1"
                     /translation="MKSPCEEIVWDVLPSIRAAIAEELIKRGISQKDVSKMLGITPPA
                     VSQYVSKKRGYNIEFREDIREAIGGLAEDLIDGKVDDLVMRICDICRMLQEDESACVS
                     ARCKGK"
     gene            1131..>1983
                     /locus_tag="PLV49_06655"
     CDS             1131..>1983
                     /locus_tag="PLV49_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG98559.1"
                     /translation="MHQTHMIINGEQLPISSHRVETIYNPANQEPVALVSVGTRQDAQ
                     TALEAAKKAFPLWSETSSEKRAELLHSAADLVRERAVSIARLLTQEQGKPLKNARAEI
                     LSSADILDYYAEEGRRNVGEWLATSRGRSVVLRQPVGVAALITPWNYPVDLLSWKVGP
                     CLAAGCTFVAKPPSRAPVAATEFVRAISDAGLPAGVVNVVHGPGGEVGAELVENPISR
                     KIAFTGETETGRWIMAQAANQIKRVSLELGGHSPFIVCGDADIEKAAAACAQRAFSNM
                     GQICISVN"
BASE COUNT          441 a          553 c          530 g          459 t
ORIGIN      
        1 tgcccggtct atagtcatcg aggtcgtcgg cgttcacccc atcggcgatg cagcttatac
       61 cctctttctg ggccaccctc ttgagaatgg cactggacct ctttttgcag atatagcagc
      121 gggtggctgg attttgagaa aaagcctcat cctcgagaat ggagaacctc tccaccctca
      181 gtctcagacc gagggattct gccagggcaa ccgcctcatt cagctcgctt ctggaatatg
      241 cctcgctgtc caggatcacc gccagggccc cctcacccaa gacatcatga gccagctttg
      301 ccaggaggct gctgtccacc ccaccggaat acgagatgag aagccgtcct ttctcccgga
      361 tacgatcttt gagatctgtt agcttgtcgt cagggctcat ttttaacatt ccaaaaatat
      421 tatttcttcc tggaggagag agaatatgca tactgaatga tgcattggga ggtctgtgga
      481 aaggcatatg aagaaagatg atttgaatat gcccttactt gcctttgcat ctggcggaga
      541 cgcaagcgct ctcatcctct tgaagcatcc ggcagatatc acagatcctc atcaccagat
      601 cgtcgacctt tccatctata agatcctccg caagccctcc tatcgcctcc ctgatatcct
      661 ctctgaactc gatattgtag cccctcttct tcgagacata ctgggatacc gcaggggggg
      721 tgattcccag cattttggac acatccttct gagaaattcc tcttttgatc agttcttcgg
      781 caatagccgc tctgatgctg ggaagcacat cccagacgat ctcctcacat ggcgacttca
      841 tctataatga acctcaaatc gatgtttatt cgatcattat cttagccttc catcatactt
      901 atacccatgt caacgaccct gccctgaagg acggggcctg caactacgtt gatattgtgt
      961 cgcgtttggc tggttgacat tgcagcctgc tacggtcaga tctactgaag gcagcgatgg
     1021 gcgatgaatg aggcgattca ttctgatcgg tccggcccaa aggttatcag ccaggaggga
     1081 tagaatcggt gatagaatag ttgatggaat aagcgacgcg gaggtctttc atgcatcaga
     1141 ctcatatgat aatcaacggg gaacagctcc ccatttcatc ccacagggtg gagacgattt
     1201 acaatccagc aaaccaggag cctgtggctc tggtctccgt aggaactcgc caggatgccc
     1261 agacggcttt ggaggcggca aagaaggcat ttcctctgtg gtctgagacc tccagcgaga
     1321 agagggcaga gctattgcat tctgccgcgg atctagtgcg agagagggcg gtgtccatag
     1381 cccgactgct gacccaggag cagggaaagc ccctgaaaaa tgcccgcgcg gagatcctat
     1441 catctgctga tatcctggac tattatgcag aagagggcag gaggaacgtc ggggaatggc
     1501 tggccacatc ccgcggccgg agcgttgtcc tccgccagcc tgtgggcgtc gctgccctca
     1561 tcaccccctg gaattatccg gtggacctcc tctcctggaa ggtggggccg tgcctggctg
     1621 caggctgcac ttttgtggcc aaaccgccca gccgcgctcc cgtcgccgcc acggaattcg
     1681 ttcgggctat ttctgacgcc ggtcttcctg caggagtggt caatgtggtc cacggtccgg
     1741 gtggagaggt gggagcagag ctggtggaaa atcccatctc caggaagatc gccttcacag
     1801 gcgagaccga gacaggacga tggataatgg cccaggctgc caatcagatc aagcgggtct
     1861 cactggagct gggagggcac tcgcccttca ttgtctgtgg cgatgccgat atcgaaaaag
     1921 cggctgcagc ctgcgcccag cgggccttct ccaatatggg gcagatctgc atcagcgtca
     1981 acc
//
LOCUS       DAOSGP010000259         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_57773_length_1974_cov_14.779573, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000259 DAOSGP010000000
VERSION     DAOSGP010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57773_length_1974_cov_14.779573"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1520)
                     /locus_tag="PLV49_06660"
     CDS             complement(219..1520)
                     /locus_tag="PLV49_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOG98560.1"
                     /translation="MAPDFQAVDLDGKNVSLSDYRGEAVLLHITNIENPLCRECENAL
                     NAQTNQLSRLAEENPGANIITLNVRKNSFSLDGRALAEDWWKANITWPWIEDFDPYPL
                     TGKYIDYSTLEGGFANPTLILIDKEGNVAGLYHVYQLGKGEIDGIQSAESLKSDLERI
                     EAGEASGFEGIASSQGINYLGMFALGIVTSLSPCSIALLLAMFSYVMTARRKEEYLRK
                     TASASKEGFMIGIAFTLGMAAVFFIVGFFLSDIGVFIRQARFFDLAAGLLMIILGINI
                     IKPIGEIIEPVRSRLSFGRGNSKAIDEETIAEKKGIMERLICISMNLFRYSAFIGAFT
                     LGVFFAMGWAPCAVSLVFPVLIWLISQDVTPIGGALMLFVFGVGHGVPVIPIATFSRA
                     VGGQIGEKYIAAGKHVTKIFGLAVIVIGLIYAVRYLGFKMW"
     gene            complement(1659..>1974)
                     /locus_tag="PLV49_06665"
     CDS             complement(1659..>1974)
                     /locus_tag="PLV49_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG98561.1"
                     /translation="CDYCVPQTEAVQKIIDEFDGRITIFEMGADGSDERTTEAMQAYD
                     PDGGTMYVPLTIILTLAPDSEGEVVAVWHSTDKVTGEEWIRNYVEDAISLYDENSADW
                     DN"
BASE COUNT          483 a          545 c          496 g          450 t
ORIGIN      
        1 atggaccttc tggcctgctg caatcccaag gacctctccc agtgcgacag cgtgcagagg
       61 gccagggaca tactggaaag attgaggtat ctggccaggg agagtctgga agacgatagg
      121 aaataattcc aaaatcccct gcaatataaa ccgcttttcg ataatgcaaa agcccagcgg
      181 gaggtggcct cctcaccgcg gggcatactc ttttcgcatt accacatctt gaaccccaga
      241 tacctcaccg catagatcaa tccgatgacg atcaccgcca gaccgaatat ctttgtgaca
      301 tgctttccag cagcgatata cttctctccg atctgccctc caactgccct ggagaaggta
      361 gcgatgggta tcaccggcac tccatggccc accccgaaga caaagagcat cagggctccg
      421 cctattggcg tcacgtcctg ggatataagc cagatgagca ccggaaagac cagggacacg
      481 gcacacggcg cccagcccat ggcgaagaag acccccagag tgaaggctcc tatgaatgcc
      541 gagtaccgga agaggttcat ggagatacag atcagcctct ccatgatccc cttcttctcc
      601 gctatcgtct cctcatcaat cgctttggaa ttgcctctgc caaaagaaag gcgtgagcgc
      661 accggctcga tgatctcccc aatgggcttg atgatattta tccccaggat gatcatcaat
      721 agacccgcag ccaggtcaaa gaagcgggcc tggcggatga aaacgccgat atccgagagg
      781 aaaaagccca cgatgaagaa tactgccgcc atgcccaggg tgaaggctat gccgatcata
      841 aagccctctt ttgatgccga ggcggtcttt ctcagatact cctccttcct gcgggcggtc
      901 ataacatagg agaacatggc caagagaagg gctatggagc agggggagag ggaggtgaca
      961 attcccaggg cgaacatgcc cagatagttt atgccctggc tggaggcgat tccctcgaat
     1021 ccggaggcct cgcctgcctc tattctctcc aggtcgcttt tcaacgactc agcactctgg
     1081 atgccgtcta tctccccctt gcccagttga tagacgtggt agagacctgc cacatttccc
     1141 tctttatcga tcaagatcag ggtgggattg gcaaatcctc cctccagagt tgagtagtcg
     1201 atgtacttgc cggtgagggg atacgggtcg aaatcctcta tccagggcca ggtgatattg
     1261 gccttccacc aatcctcagc tagtgctcgc ccatccaatg agaaggaatt cttccgcaca
     1321 ttgagggtga tgatgttcgc gccgggattc tcttccgcca gcctggatag ctggttggtc
     1381 tgggcattaa gagcattctc gcactcccgg cagaggggat tttctatgtt ggtgatatgg
     1441 agcaggactg cctctcctcg atagtcagag agagagacat tttttccatc aagatccact
     1501 gcttgaaaat caggggccat agctatctgg gaaagagcag gggatgaaca cattaagaag
     1561 atggaaatta agacagcaaa aagtgtgacg gaccccgata tcagggattt cttacatttc
     1621 gtggacatgg caacgatccc tttatctgag atcctgctct aattatccca gtctgcggag
     1681 ttctcatcat ataggcttat ggcgtcctcc acatagttcc tgatccactc ttcgcctgtt
     1741 actttatctg tgctgtgcca tactgcaacg acctcgcctt cagaatcagg agccaaagtc
     1801 aatatgatgg tcagagggac atacatagtg ccgccatcgg gatcataggc ctgcatggct
     1861 tcggtagtcc tctcatcact gccatcggca cccatctcga agatcgtgat cctcccgtcg
     1921 aattcgtcga ttatcttctg cacggcctcg gtctgtggta cacaataatc acat
//
LOCUS       DAOSGP010000260         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_58031_length_1968_cov_20.414532, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000260 DAOSGP010000000
VERSION     DAOSGP010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58031_length_1968_cov_20.414532"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1232
                     /locus_tag="PLV49_06670"
     CDS             132..1232
                     /locus_tag="PLV49_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG98562.1"
                     /translation="MRSIQKTQSKPQGAKPERGKAVGAKFLLLRPAGYPLKSIFQECP
                     SVADARLFELYAREQWYGEEVNPGCYLFDRRLYPDYAFQVVRAYPRRSVVGFETTIKV
                     EQKAEVKKGVSYDVSFDEVVGQEEAKRKVKIVEKYLLDPEKFGRWAPRNILFYGVSGT
                     GKTMIAKALSAKAGVPMLAVKSTSLIGEFVGEGARQIHSLYEKAEEMAPCIIFIDELD
                     AIALDRRYQDLRGDVSEMVNSLLTEMDGISSRLGICTIAATNQIEVLDSSIRSRFEEE
                     IEFKLPDKDERLQILEKNASTLPLEMVDVDLKEIARQTDGFSGRDLVEKVLKVALHHA
                     LMDECNISQKHIEEALPRARKHYRQPPTEMFS"
     gene            1291..1779
                     /locus_tag="PLV49_06675"
     CDS             1291..1779
                     /locus_tag="PLV49_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOG98563.1"
                     /translation="MDKFKRFPAQEKVIRLVLQRGFQINDQARVVSGRIEIPHAQIAK
                     ELEVDRRVVDTTAQAIREDEVLWKVFRNVRSMIYLGEVAPILGLGVIEITPVNAVKTG
                     LLGDVASAVARNGLSIRQAVSDDPYFVESPKLTIITEGKIPGDLVILLKEIEGVKRVT
                     VY"
BASE COUNT          492 a          483 c          555 g          438 t
ORIGIN      
        1 ggcgacaaga ggggaggcaa aaagaagggt cttctcgata cctgccagtg agctgctggc
       61 taagctcaac tggagattct ttttttcagc gataaaaaga tttaactatc tccgtcagca
      121 agaaccatat tgtgcgatca atccagaaga cccagtccaa gcctcagggg gcaaagccgg
      181 agaggggaaa agcagtaggg gcaaagtttc tcctcttgcg gcctgccggc tatcccttga
      241 agagcatctt tcaggagtgt ccatccgttg ctgatgccag gctctttgag ctgtacgccc
      301 gggagcaatg gtacggtgaa gaggtaaacc ctggctgcta cctcttcgac cgcaggctct
      361 atccagacta tgcctttcag gtggttcggg cctatcccag gcgctctgtg gtcggctttg
      421 aaaccaccat caaggtggag cagaaagcgg aggtcaagaa aggggtaagc tacgatgtct
      481 cctttgatga ggtcgtaggt caggaggagg ccaagcgcaa ggtgaagatc gtggagaagt
      541 acctcctgga tccggagaag ttcgggcgct gggcaccgcg caacatcctc ttttatggcg
      601 tatccggcac cggaaagacc atgatcgcca aagcactctc tgctaaagct ggtgtgccga
      661 tgctcgctgt caagtccacc agcctgatcg gcgagttcgt gggcgaggga gccaggcaga
      721 tccacagcct ttatgagaag gcggaggaga tggcgccatg cataatcttc atcgatgaac
      781 tggatgccat tgccctggac cgacgctacc aggacctgag aggcgatgtc tctgagatgg
      841 tcaactctct gcttacggag atggatggaa tatccagcag gctgggcatc tgcaccattg
      901 ccgccaccaa tcagattgag gtcctggact cttccattcg ctcccgtttt gaggaggaga
      961 tcgagttcaa gctgccggat aaagatgagc gcttgcagat tctggagaag aatgccagca
     1021 ccctgcccct ggagatggtt gatgtggacc tgaaggagat cgccaggcag acggatggct
     1081 tctccgggcg agatctggtg gagaaggtgc tgaaggtggc actgcaccat gctctcatgg
     1141 atgaatgcaa tatatctcaa aagcatatcg aagaggcatt accaagagcg cgaaagcatt
     1201 accgacagcc tccgacggaa atgttctcat aaggaagccc ttttgtactt ataagtccaa
     1261 tatggcttga ggcacatgtg gcaggagatt ttggataagt tcaagaggtt tcccgcgcag
     1321 gagaaggtca tccgcctcgt cctgcagagg ggctttcaga tcaacgatca ggcccgggtg
     1381 gtatcgggcc ggatagagat tcctcatgct cagatcgcaa aggagctgga agtggacaga
     1441 agagtggtgg acaccacggc acaagccatt cgcgaggatg aggtcctctg gaaggtcttc
     1501 agaaatgtgc gctccatgat ctatctgggt gaggtggcac ccatcctggg attgggtgtt
     1561 atcgagatca ctcccgtgaa tgccgtcaag acagggcttt taggcgatgt ggccagcgct
     1621 gtggccagga acggcctctc catccgccag gctgtctcag acgatccata cttcgtagag
     1681 agcccaaagc tcaccatcat cactgagggg aagatcccag gcgatctggt cattctgctc
     1741 aaggagatcg agggcgtgaa aagggtgacc gtatattaaa cctcaaaatt tcgttggcct
     1801 attactatca acaatatcgg caatatcgtt attaccatta tgattatttc aaaggaaaaa
     1861 tgcgaccgct gcacaattaa ttaaacttaa atagtttaaa atacaagtta tttcttggag
     1921 cctccggtga tatcccttca tcccttctct cgaaggctct aacccccc
//
LOCUS       DAOSGP010000261         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_58123_length_1966_cov_21.194139, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000261 DAOSGP010000000
VERSION     DAOSGP010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58123_length_1966_cov_21.194139"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1860)
                     /locus_tag="PLV49_06680"
     CDS             complement(1..1860)
                     /locus_tag="PLV49_06680"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOG98564.1"
                     /translation="MLLIHSDFIEFEAKRPTKLAEEVEDSAKKGRLDEALCAFIAVEK
                     FDEDDTDAVIAEGAKQVADVASQVKANRIMIYPYAHLSSSLSTPATAIKILKGMADVL
                     SENYEVMRAPFGWYKAFTISCKGHPLSELSRSIRLGEGEVVSEALKSEAKAVSYWRVL
                     DLDGKLVDADKFNFEGHTNLQKFANYEISKARAVDKVPPHVELMRRLELVDYEPGSDS
                     GNMRYYPKGRLVKSLLETLVLEKAADMGAMEVETPIMYDMEHPTLKKYLDRFPARQYQ
                     IDSDKRKLFLRFAACFGQFLMGHDMSISYKSLPLKMIEMTRYSFRREQRGELVGIRRL
                     RAFTMPDMHTMTRDMYSAIEEFRKQYLASISVLKEVGLEVEDYEVAIRFTRDFYKENQ
                     EFIAGLVDIIKKPVLIEMWDERFFYFVLKFEFNFVDTMDKASALSTVQIDVENAERYD
                     INYIDESGARRRPILLHCSPSGAIERVMYAILEKAARLSLQGKLPMLETWLSPTQVRI
                     IPVAERHQERALALAGILGFRTDIDDRDETVGKKIRDAGREWVPYVAVIGDEELESGR
                     LAVTIRSESTPKSPAKKSMTVDELKERLTGETKGKPWRRLPMAMRLSERPRFI"
BASE COUNT          388 a          585 c          534 g          459 t
ORIGIN      
        1 tcagatgaac ctgggcctct ccgagagcct catggccata ggcagtctgc gccagggctt
       61 tccttttgtc tctccagtga gcctctcttt gagctcatcc acagtcatgc tcttctttgc
      121 cggcgacttg ggggtggatt cgctgcgaat ggtgacggcc agccggcccg actcgagctc
      181 ctcgtccccg atcactgcca catatggcac ccattccctt ccagcatcgc ggatcttctt
      241 gcccacggtc tcgtccctgt catctatatc cgtgcggaag ccaagaatcc cggccagggc
      301 aagagccctc tcctggtggc gctcagccac cggtatgatc ctcacctggg ttggagacag
      361 ccaggtctcg agcatgggca gcttgccctg aagagatagt cgggccgcct tctccagtat
      421 ggcgtacatc accctctcta tcgctccgga gggagagcaa tgcaagagta tgggccgcct
      481 tctcgcacca gactcatcga tatagttgat atcgtagcgc tccgcattct ccacatcgat
      541 ctggacggtg gagagggcgc tggccttatc catggtatcc acgaagttga actcgaattt
      601 gaggacgaaa tagaagaacc tctcatccca catctcgatt aagaccggct ttttgatgat
      661 atccaccaat ccagcaatga actcctggtt ctccttataa aagtcccgag tgaacctgat
      721 ggccacctca tagtcttcaa cctccaggcc cacctccttc aggacgctga tgctggcgag
      781 atactgcttc ctgaactcct caatggcgga atacatatcg cgggtcatgg tgtgcatatc
      841 gggcatggtg aaagcccgca ggcggcggat gcccaccagc tccccccgct gctcgcgccg
      901 gaagctgtat ctggtcatct ctatcatctt caggggcagg gacttgtagg atatggacat
      961 gtcatggccc atcaagaact ggccgaagca ggcggcgaaa cggaggaaaa gctttctctt
     1021 gtccgagtcg atctgatact gccgggcggg aaagcggtcc agatactttt tcagagtggg
     1081 atgctccata tcgtacatga tgggggtctc cacctccatg gcgcccatgt ccgccgcctt
     1141 ctccagtacc agggtctcca ggagggactt gaccagcctg cctttggggt agtagcgcat
     1201 gttgcccgag tcggagcccg gctcgtagtc caccagctcc agccgtctca tcagctccac
     1261 atggggcggg accttgtcca ctgctctcgc cttggagatc tcgtagttgg cgaacttctg
     1321 caggttagtg tggccctcga aattgaactt gtctgcgtcc accagctttc catccagatc
     1381 taagaccctc cagtaggata ccgctttggc ctcagacttc agggcctcgg ataccacctc
     1441 cccctcgccc agccttatgg atcgagagag ctcggagagc ggatggccct tgcagctgat
     1501 ggtgaaggct ttgtaccagc cgaagggagc gcgcatgacc tcatagttct cggagaggac
     1561 atctgccatt cccttgagta tcttgattgc cgtggccggg gtggagaggc tggagctcaa
     1621 gtgggcatag ggatagatca taatgcggtt ggccttgacc tggctggcca catctgctac
     1681 ctgctttgcg ccctcggcga tcacagcatc ggtatcgtcc tcgtcgaact tctccacggc
     1741 gatgaaagcg cacagcgcct catcgaggcg gcctttcttg gccgaatctt cgacctcttc
     1801 tgccagcttc gtgggcctct tggcctcgaa ctcaatgaaa tccgagtgaa ttaggagcaa
     1861 ttgcaatatc ggtcacctga actcttgagg gatgagtgtg ggtgtaattc aatgtttcta
     1921 tctagcccca ggtgctcaga ggagaaaggc tcctgctgcc agggtg
//
LOCUS       DAOSGP010000262         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_58620_length_1955_cov_8.690000, whole genome shotgun sequence.
ACCESSION   DAOSGP010000262 DAOSGP010000000
VERSION     DAOSGP010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58620_length_1955_cov_8.690000"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="PLV49_06685"
     CDS             complement(<1..1797)
                     /locus_tag="PLV49_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HOG98565.1"
                     /translation="MIFQIIDANYTYDPRKRPLVQLFGTTEAGESVTCRVDGFRPYFY
                     AWVEEDRLKKAALELETMGLEVEVVKRFEPIGFQTSPKTMLKIITHDPKEMRSLREKV
                     KEVEGVRAVYETDILFKNRFLIDCHLGGMSWVDAPLPEWLAGQKAAQPGSSSSSHCPL
                     VDVQSLLAVEHEANAPLRFMSFDIECLPDHGEMPRPESSPVILISMAFFPPYQGNSDL
                     VLVGKEIECPRGDTISCRDEYDLISRFFSIIRDYNPDILAGYNSNEFDFPYLAERAKQ
                     LRLEARVGRDGSSWYIRKIVSRTNVSITGRVIVDLLPIIRSAYSLKQYTLRNAAAELL
                     KMKKHDVDPKEIKSLWEQGGEGLRRFVSYSRRDAVLVQRMLIDLRLMDKYIALSRASG
                     SLLQDIVNGGQSGMVESLLLRRFRERGRVVPSKPDSDISEGRYEENEALKGGAVLVPE
                     KGLVEDVIILDYKSLYPTIMMAHNLCYSTVLVSPDSTEESHMAEREMGDKEIITSPSG
                     GRFVSSQVSPGIMPAILRELLEQRTATKRLMKKAGNEERLFLDAKQYAMKILLNSFYG
                     YSGYARARLYSLALANAVTSFGRENILRTKKII"
BASE COUNT          413 a          546 c          494 g          502 t
ORIGIN      
        1 tatgatcttt ttggtgcgca ggatgttctc ccgaccgaag gaggtgacgg cattggctaa
       61 agccaggctg tagagccgtg ctctggcata tcctgagtag ccgtaaaagc tgttgagtag
      121 tatcttcatg gcatactgct tagcatccag aaaaagccgt tcctcgttgc ccgccttctt
      181 cattagcctt ttggtcgccg tcctttgctc caacagttca cggaggatgg caggcatgat
      241 tcccggcgag acttgagagg agacgaacct cccccctgaa ggagaggtta tgatctcttt
      301 atcacccatc tcccgttctg ccatatggct ctcctccgta gagtcggggc taacaaggac
      361 tgtggaatag cagaggttat gggccatcat gatggttgga tagagtgatt tgtaatccag
      421 aatgatcaca tcctcaacca gacccttctc cggtaccagg acggcacctc ctttcagcgc
      481 ctcgttctcc tcatatctcc cctccgagat atcggaatcc ggtttggatg gaaccaccct
      541 tccccgctcc cgaaagcgac gcaagagtag gctctccacc atacccgact ggccgccgtt
      601 aactatgtcc tggagcaagg aaccgctggc acgggatagg gcgatgtact tgtccatcag
      661 ccgcaggtcg atgagcattc tctggaccag gacggcatct cttcgggagt agctgacgaa
      721 gcggcgcagg ccttcccctc cctgctccca gagagacttg atctccttgg ggtccacatc
      781 atgcttcttc atctttagaa gttcggcggc ggcgttgcgt agagtgtact gcttgaggct
      841 gtaagcagag cggattatgg gcagaaggtc tactatcacc cgaccggtta tggagacatt
      901 ggtccggctc actatctttc gtatgtacca tgagcttccg tccctgccca ccctggcctc
      961 caggcgcagc tgtttggctc tctccgccag atagggaaag tcgaattcat tggagttgta
     1021 gccagccagg atgtccgggt tatagtccct gattatggaa aagaaccggg agataagatc
     1081 gtattcatcc ctgcatgaga ttgtatctcc ccgtgggcac tcgatctcct ttcctacaag
     1141 taccagatcc gagtttccct gatagggtgg gaagaaggcc atgctgatca gtatcaccgg
     1201 tgagctttcg ggcctcggca tctccccatg atcgggcagg cactcgatat caaagctcat
     1261 gaagcgcagg ggggcattgg cctcatgctc cactgccaag agagattgca catccaccag
     1321 gggacaatga gatgaagatg acgagcctgg ctgggcagcc ttctggccag ccagccactc
     1381 cggtagtggt gcatccaccc agctcatgcc tcccagatgg caatctatga gaaacctgtt
     1441 tttgaagagt atgtctgtct cgtagaccgc ccgcacaccc tccacctctt tgaccttctc
     1501 cctgaggctt ctcatctcct tgggatcgtg ggttatgatc ttgagcatcg tcttgggaga
     1561 ggtctgaaag ccgataggct caaatcgctt cactacctca acctccagcc ccatcgtctc
     1621 cagctcaaga gcggccttct tcagacgatc ttcctctacc caggcataga agtagggacg
     1681 aaagccatcc acgcggcagg ttacgctctc ccctgcctcc gtggtgccga agagctgcac
     1741 cagaggcctc ttccgagggt cgtaggtgta attggcatct atgatctgga atatcatggt
     1801 ttaaaattga gatcgcggct tatataactt gcctgcttcc tttgatgtat cacagtattt
     1861 tcgtgtatat ggtgtttttt gcacgttgtt gttacttcca tttatttcca tcctctcctc
     1921 tcaaattcct tgaatctctc tattgtgtga atgaa
//
LOCUS       DAOSGP010000263         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_58755_length_1952_cov_23.899315, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000263 DAOSGP010000000
VERSION     DAOSGP010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58755_length_1952_cov_23.899315"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLV49_06690"
     CDS             <1..343
                     /locus_tag="PLV49_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98566.1"
                     /translation="MSDTEAKSSIGPGMGLIHLETGVQIIIPAQIEPERIVAAIEALL
                     AAVKDNMSEGIGCSDIAWMTSSDVNAMLLVSTDMEEGPAGAVSALHAAAEAFSERSGR
                     VISQMEADSGE"
     gene            340..>1952
                     /locus_tag="PLV49_06695"
     CDS             340..>1952
                     /locus_tag="PLV49_06695"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOG98567.1"
                     /translation="MKIALLQLNPTIGDLAGNKDLIARAIRRAPDFDLAVTSELVLPG
                     YPPCDLLLNEEFVERSWQALDELAEELADLPPVLVGLAEPDTSAGARPLFNSAALIRG
                     GKVTETFQKSMLSGCDLFHEGRYFQRRKEPRVLQIEGKSLGILIGELLLGSGDNERQD
                     RPDPIAELRAKGADAIVNLSASPFTAGKQLLREARLSDLARKHCLPILSVNQVGGNDD
                     LIFDGRSCFFDAAGRLIARGRGFEEDIIVADLDEPKEIIATDDFTFEAEIWRALVLGT
                     RDYVHKCGFSSVLLGLSGGIDSSLVAAIAVQALGPNNVLGVLLPSPYTRQSSIDDALA
                     LAANLGIKTISILLTDIMGSFELELAEHFAGRPKDITEENIQARIRATLLMALSNKYG
                     SILLATGNRSELSVGYCTIYGDMAGGLAVISDLPKTMVYRIAEWLNSTASKPVIPPSI
                     IKKPPSAELRPGQTDQDSLPPYPLLDEILKRYIDEHQSRADLLRAGLDETMVDRVISL
                     VKNAEFKRVQAALGLKVIDKSFGEGWRMPV"
BASE COUNT          413 a          552 c          574 g          413 t
ORIGIN      
        1 catgagcgat acagaggcaa agtccagcat cggcccgggc atgggcctga tccatctgga
       61 gactggggtg cagatcatca tccccgccca gatcgagccg gagaggattg tggcagcaat
      121 agaagctcta ctggctgcag tcaaggacaa tatgagcgag ggcataggct gctcggatat
      181 cgcctggatg acctcctcgg atgtcaatgc catgctcctg gtgagcactg atatggagga
      241 ggggccagca ggcgcagttt ccgcccttca tgccgcagcg gaggccttct ccgaaaggtc
      301 cggtcgggtg atatcgcaga tggaggcaga ttccggggaa tgaaaatcgc cttattgcag
      361 ctaaatccca ccattggaga cctggccgga aataaggatc tcatcgcccg ggccattcgt
      421 cgcgcgccgg atttcgattt agccgtcaca tccgagctcg tccttcctgg ctatccgcca
      481 tgcgatctac ttctcaatga ggaatttgta gagcggagct ggcaggctct ggacgagctg
      541 gccgaagagc tggctgacct ccctccggtt ctggtgggcc tggcagagcc ggacacctcc
      601 gccggggcgc gccctctttt caactctgct gccctgatcc ggggagggaa ggtgactgag
      661 acctttcaga agagcatgct ctctggttgc gatctattcc acgagggccg ctacttccag
      721 cggagaaagg agcctcgggt tctgcagatc gagggcaaat ccctgggcat tctgataggt
      781 gagcttcttt tgggcagcgg cgataacgaa aggcaggatc ggcccgatcc cattgcagag
      841 cttcgggcca aaggcgctga cgctatagtc aacctgtccg cctccccctt caccgcggga
      901 aagcagctcc ttcgggaggc aaggctatca gatctcgcca ggaagcactg cctgcccatc
      961 ctttctgtca atcaggtggg cgggaacgat gatctgatat tcgacggtag aagctgcttt
     1021 tttgatgccg caggccgcct tattgccaga ggacggggat ttgaggagga tatcatagta
     1081 gcagacctgg atgagccaaa agagatcata gctactgacg acttcacttt cgaggcggag
     1141 atctggaggg ccctggtcct ggggacgagg gattatgtgc ataaatgcgg cttttccagc
     1201 gtcctcctgg ggctttctgg agggatcgac tcctccctgg tggcagccat agccgttcag
     1261 gccctggggc cgaataacgt cctgggcgtt cttctgccct cgccttatac tcgccagagc
     1321 agcatcgacg atgccctggc cttagctgcc aacctgggca tcaagactat ctccatcctt
     1381 cttaccgata tcatgggctc ttttgagttg gagctggcag agcatttcgc tggaaggccg
     1441 aaggacatca ctgaagagaa catccaggcc agaatccgcg ccacactcct catggccctg
     1501 tccaataagt acggctctat ccttctggct accgggaaca ggtcagagct ctccgtgggc
     1561 tactgcacca tctatggcga tatggccggg ggtctggccg tcatctccga cctgcccaag
     1621 acaatggtct acaggattgc ggaatggctc aactccaccg ccagcaaacc ggtcattccc
     1681 ccgagcatca ttaagaagcc tccttcggca gagctccggc ccggtcagac cgatcaggat
     1741 agcctgccgc cttatcccct gctggatgaa atcctcaagc gttatatcga tgagcaccaa
     1801 tcccgggcgg atctgcttcg agccggattg gacgaaacga tggtggacag ggtcatatcc
     1861 ctggtcaaga acgctgaatt caagagggtt caggccgctc tcggactcaa ggtgatcgat
     1921 aaaagctttg gagagggctg gaggatgcct gt
//
LOCUS       DAOSGP010000264         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_59036_length_1946_cov_18.701216, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000264 DAOSGP010000000
VERSION     DAOSGP010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59036_length_1946_cov_18.701216"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..450
                     /locus_tag="PLV49_06700"
     CDS             13..450
                     /locus_tag="PLV49_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG98568.1"
                     /translation="MPVYVDISRCMGCRSCEVACDRVHGGKGHVNVHFVGSLASVPVF
                     CHHCEEACCTMVCSTGALHKEGDKTAFDVDKCTGCGLCQLACPFGVVRTDKIAHKCDL
                     CSGQEMPTCVMTCPANALSLDFDLASRRARNRAAMLIARGGRA"
     gene            447..866
                     /locus_tag="PLV49_06705"
     CDS             447..866
                     /locus_tag="PLV49_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG98569.1"
                     /translation="MIKVDRGMCLGCLSCSNVCPNQNIVRTDTGESRTVHWKRCKEEC
                     DLCVEFCPAKALTLVPYDELVPESEVSFELVACRVCGSRYATLPMLMKVEASLPAGMQ
                     EDASGLEWIRICSQCRRDIEAEKAAGQVMKERKRISP"
     gene            complement(915..>1946)
                     /locus_tag="PLV49_06710"
     CDS             complement(915..>1946)
                     /locus_tag="PLV49_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOG98570.1"
                     /translation="EYEFQPFVPGQCIKTLGLEKDYQLTPVSGKVTVTAPGRIHLTVL
                     DMNRFAPNRPGGGGMGFALQIYCSAQVECIPSGLEIDYSREPIIRHFAEVFRKTVRYE
                     GGFRIVVRDHQKKHVGLGSTGSVLLALGHAMNTALGSPLNREQIRMLIGHNYVEETED
                     GRVTNGFETGVGPAAATYGGMVITGDELALVYQHRFAEGKNVFITIPPTIVSSAGRSE
                     FDLLMNRARNLDYQDRELKAYLMLMDLIPALEKSDLRKIGDTIWEIEFRGSKRAEVEH
                     HGFEIYRYMSILRDADLEFVGMSSVGPSIAIVTERSRDEVAKIIEPLGLKIAVETKVD
                     NVGLTIRVD"
BASE COUNT          399 a          520 c          565 g          462 t
ORIGIN      
        1 aagagggatt agatgccagt ttatgttgat atcagcagat gtatgggatg ccggtcctgc
       61 gaggtggcct gcgatcgggt gcatggtggc aaagggcatg tgaatgttca ttttgtagga
      121 agtctggcat cagttccggt cttctgccat cactgtgagg aggcctgctg caccatggtt
      181 tgcagtaccg gagccctgca taaagagggg gataaaacgg cctttgatgt ggacaagtgc
      241 actggctgtg gcctgtgcca gctggcctgt ccctttgggg tggtacggac ggacaaaatc
      301 gctcataaat gcgatctgtg ctcgggccag gagatgccga catgtgttat gacctgtcca
      361 gccaatgccc tgagtcttga ttttgatctt gccagccgca gggctcgcaa ccgtgcggcc
      421 atgttaatcg cccggggagg tagagcatga taaaggtcga tagaggaatg tgtctgggct
      481 gcctttcctg ctccaatgtc tgccccaacc agaacatagt ccggacagat actggcgaat
      541 ccagaacagt tcactggaag aggtgtaagg aggagtgcga tctctgcgtc gagttctgcc
      601 cggccaaagc actgactctt gtgccctatg atgagcttgt cccagagtcc gaggtctcct
      661 ttgagctggt ggcctgcagg gtctgcggat cacgctatgc caccttgccc atgttgatga
      721 aggttgaggc ttcccttccc gcagggatgc aggaggatgc cagcggcctg gaatggatcc
      781 ggatctgttc ccaatgccgg cgggatattg aggcggagaa ggcggctgga caggtgatga
      841 aggagagaaa gaggattagc ccctgaagga ggatccgatt cgctccttca ggctggtttt
      901 atcctatgtc ttgcctagtc caccctgatc gtcaggccta cattgtccac tttggtctct
      961 acggcgatct tcaagcccaa cggttcgata atcttcgcca cctcatcccg gctgcgttcg
     1021 gtgactatgg cgatggaagg tccaaccgag ctcatcccca cgaactccag gtcggcatct
     1081 cgcagaatgc tcatgtagcg gtagatctca aagccgtgat gctccacctc ggccctcttc
     1141 gagccccgaa actctatctc ccagattgtg tcgcctatct ttctcaggtc gcttttctcc
     1201 agcgcgggga tgaggtccat gagcatcaga taggctttta gctcccggtc ctgataatcc
     1261 aggttcctgg cccggttcat gagcagatcg aactccgagc ggccagcaga ggagacgatg
     1321 gtggggggga tggtgatgaa gacgttcttc ccttcggcaa accggtgctg gtagaccagg
     1381 gccagttcgt cgccggtaat taccatgcct ccgtaagttg ctgccgctgg acccacgccg
     1441 gtctcaaaac cgtttgtaac cctgccgtcc tcggtctcct ccacatagtt gtggcctatg
     1501 agcatgcgta tctgttctct attcaggggg gagcccaaag cggtgttcat ggcatggcct
     1561 aaagctaaaa ggaccgatcc ggtggagccg agtcctacat gctttttctg gtggtcgcgg
     1621 accacaattc tgaatccacc ctcatagcgg accgtctttc tgaatacctc tgcaaaatgg
     1681 cggattatag gttcccgaga atagtcgatc tccaacccgg atgggatgca ttccacctgg
     1741 gcagagcagt agatctgcag ggcaaagccc attcctcctc cccccggccg gttgggggca
     1801 aagcggttca tgtccagcac agtcagatga atgcgaccag gagcggttac cgttaccttc
     1861 cctgaaaccg gagtgagctg gtagtctttt tccaatccca gggtttttat gcactggccc
     1921 gggacaaagg gctggaactc gtattc
//
LOCUS       DAOSGP010000265         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_59075_length_1945_cov_20.079365, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000265 DAOSGP010000000
VERSION     DAOSGP010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59075_length_1945_cov_20.079365"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLV49_06715"
     CDS             complement(<1..389)
                     /locus_tag="PLV49_06715"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOG98571.1"
                     /translation="MKDLHMPDRFEILHKDLAGRIGRLSTLHGTVETPLLMPVINPHL
                     PLIPPEELASMGAEMVITNSYIINQDPDLREGAIERGLHHLLGFPGPIMTDSGAFQLS
                     VYGDIDVLPLEILDFQFAIKSDISVPL"
     gene            462..944
                     /locus_tag="PLV49_06720"
     CDS             462..944
                     /locus_tag="PLV49_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98572.1"
                     /translation="MKNITALVTLFCLMGLAGAQGSGLGDGYSSTQRAFFNEPITSTF
                     EPYVNNYWSNYVVGNKNQSSHVMTSEMDMWVNNFPLMFSTPMQLDSTSFKSNVSTLGL
                     SEKEKNSQALKTAVYRELGLNDLGKFAAVAGLNATSATPAVDATGNLLSQSVMSFFNV
                     "
     gene            complement(955..>1945)
                     /locus_tag="PLV49_06725"
     CDS             complement(955..>1945)
                     /locus_tag="PLV49_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOG98573.1"
                     /translation="NGALSDLIAFGARILESSCGFCIGNSMSPCTDSVSVRTSNRNFM
                     GRSGTPSANVYLTSPVVAAAAALTGRLTDPRELDMEFPAIQMPEHFDIDDRMIIPPLA
                     PEQRARATICRGPNIGQPPVNQPLPEDIAGSVTIRVGDLITTDHIMPAGSRLKYRSNV
                     PKYAEFVFEPLDKDFFSRASSLRDQGFHNLIVAGLSYGQGSSREHAAMCPMYLGVKGV
                     IAKSIERIHAANLVNFGIVPFTFENEADWNLLPEKTNIRIPGIRRSLEKGLDSVLAHA
                     ESSRKGEEGVGRKGENKKEEELVLKMNLSRRQRMILLAGGLLPFTVMSRKENK"
BASE COUNT          453 a          484 c          518 g          490 t
ORIGIN      
        1 tccaggggca cggatatgtc tgattttatg gcaaactgaa aatcaaggat ctccagaggc
       61 agaacatcga tatcgccgta gactgagagc tgaaaggctc ctgagtcggt cattatgggc
      121 ccgggaaagc ccaggagatg gtgcaggccg cgctctattg ctccttccct caagtccgga
      181 tcctggttta ttatgtagct gttggtgata accatctccg cacccatcga ggccagctcc
      241 tctggaggga tgaggggaag atgggggttt atcacaggca tgagcagtgg tgtctcgacg
      301 gtgccatgaa gagtagaaag ccggccgatc cttccggcca gatctttatg cagtatctcg
      361 aagcggtccg gcatatgcaa atccttcacc aataaggaat aaataggttg ggtctgatca
      421 tcaggggata cggaaaactg aatataagag gattgattaa tatgaaaaat ataaccgcat
      481 tggttacact attctgcctg atgggcctag caggtgccca gggtagcggc ctgggagatg
      541 gttatagctc cactcaaagg gcattcttca atgaaccaat cacctcgacc tttgaacctt
      601 atgtgaataa ctactggagc aattatgttg tcggcaacaa gaaccagagc agccacgtca
      661 tgacctctga aatggatatg tgggtaaata acttccccct aatgttcagc acccctatgc
      721 agcttgattc cacgagcttc aagagcaatg tctcaaccct gggcctcagc gagaaagaga
      781 agaacagcca ggccctgaag accgcggtat accgtgaact gggcctgaac gatcttggca
      841 aattcgctgc tgtagcaggc ctgaatgcca ccagtgccac tcctgcagtt gatgctacag
      901 ggaatctgct ctctcagagt gtcatgtcgt tcttcaacgt ctgagcggct aatttcattt
      961 attttctttt cttgacatga ctgtaaaggg caagagtccg ccggccagga gaatcatcct
     1021 ctggcgacga gagaggttca tcttcagaac tagctcttct tcctttttat tttctccttt
     1081 ccttcccact ccttcttctc cttttctgct gctttctgca tgggcgagca cactatcgag
     1141 gcccttctct agagatctgc gaataccagg gattcttata ttcgtttttt ctggcagcag
     1201 attccagtca gcctcatttt caaaggtaaa cggcactatg ccgaagttca ccaggttagc
     1261 ggcatgtatc ctttctatgg atttggcgat cacccctttc actcccaggt acatgggaca
     1321 catggccgca tgctcgcggg atgagccctg gccatagctc aggcctgcca cgatcagatt
     1381 atggaagcct tgatcgcgga gggaggatgc ccgggagaag aagtccttat ccagtggttc
     1441 aaagacgaat tctgcatact tgggaacgtt tgagcggtat ttgagccggg acccagcagg
     1501 catgatgtga tcagttgtta tcagatctcc cacccttatg gtcacggatc ctgctatatc
     1561 ctctggaagt ggttggttga cgggaggttg gccgatgttc ggtccgcggc agatcgtagc
     1621 tcttgccctt tgctcaggtg ctagtggcgg gatgatcatc ctgtcatcga tatcgaaatg
     1681 ctcgggcatc tgaattgccg ggaactccat atccagctct cttgggtcag tcagcctgcc
     1741 ggtgagagcg gcagcagcag caactaccgg gctggtcaag tatacattgg cgctgggagt
     1801 gccggatcga cccataaaat tgcggttgct ggtgcggacg gagacggagt cggtgcaggg
     1861 agacatgctg tttccaatgc aaaatccgca ggacgattcc agtattctgg ccccgaatgc
     1921 tatcaggtcg ctgagggcgc cgttt
//
LOCUS       DAOSGP010000266         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_59368_length_1939_cov_16.078556, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000266 DAOSGP010000000
VERSION     DAOSGP010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59368_length_1939_cov_16.078556"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="PLV49_06730"
     CDS             complement(<1..1746)
                     /locus_tag="PLV49_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOG98574.1"
                     /translation="MLAHGEWMNTHLKLTEDLRWIYLDLLSIVVGISGGLGAVAFRKL
                     VELSHDFFFGFLLPLLPNSYFVVLLPVLGGLIVGPLIYKLAPEAKGDGISHIMIALQR
                     FTGDIRKRAGLVLIFTSAITLGSGGSAGREGPIALIGASAGSAIGKAIKLSARDLKVL
                     TCCGVASGIAATFNAPMGGAIFSMEVISKKFTSLDAVPILLAAVVGKAVATELIDPVP
                     EFVNPNFYFTTFDMVLCFFIGPLFGFLSFLWVKGYYFFEDRFQDLPLPDILKPAVGGL
                     TAGVCGIFFFEFGIMGVGYEGINNIFMLAARAPATDLLLLLLALGLLKMLATSSTVGS
                     GGSGGVFAPTLYIGTMFGLAGGMLAEILAPDQIANPLDYGLLGMGALFAGTAGAPLTC
                     IFMITEMTGNYSGLPSLIICCITSYTVARILLKGGSIYTIKLMRKGIYLDLPQPVLSE
                     VSVGEAMHKEVITVSPLCRISEVRDGIYRCNYTGFPVVDEGRLVGMITFDDIRRIPPH
                     EQEKMTVKEVAVRAPITINPHQSAKMAMDIMYENDVGRLAVVEKDDAQKLIGIITRSD
                     VIRAYEREMKRSQDEA"
BASE COUNT          462 a          555 c          502 g          420 t
ORIGIN      
        1 agcctcgtcc tggcttcttt tcatctccct ctcataagcc ctgatcacat ccgagcgggt
       61 gatgatgcca atcagcttct gcgcatcgtc tttttccacc actgccagcc ttccaacgtc
      121 attttcatac atgatatcca tggccatctt ggccgactgg tggggattaa tggtgatagg
      181 agccctgacc gccacctcct ttactgtcat cttctcctgc tcatgcgggg gaattctcct
      241 gatatcgtca aaggttatca tccccaccag acggccttca tccaccacag ggaaaccggt
      301 atagttgcag cggtagatgc catctctcac ctccgagatc ctgcagaggg ggctgacggt
      361 gatcacctct ttgtgcatcg cctcccccac cgatacttcg gagagcaccg gctggggaag
      421 atcgagataa atgccctttc tcatcagctt gatagtgtaa atggacccac ccttcaacag
      481 aattcgagcc acggtatagc tggtgatgca acaaataatc agagacggca agccactata
      541 gttgccggtc atctccgtga tcatgaatat acaggtgaga ggtgccccgg ctgtcccggc
      601 gaaaagagcc cccatgccca ggagcccgta gtccagagga ttggcaatct gatcgggcgc
      661 caggatctca gccagcatgc cccccgccag gccgaacatg gttccaatgt agagcgtggg
      721 cgcaaacacc ccgccgctcc ctccggatcc gacggtggag gatgttgcca gcatcttcag
      781 caagcccaaa gctaggagca gaaggagcag atcagtggct ggtgctctcg ctgccagcat
      841 gaagatgtta ttgatgccct catagcccac tcccattatg ccgaactcga agaagaatat
      901 gccgcatact cctgctgtta gcccgccaac cgccggcttc agaatgtcag gcagaggcag
      961 atcctggaat cggtcctcaa agaagtagta gcctttcacc cataggaagg agagaaatcc
     1021 aaacagaggc ccgatgaaaa agcagagaac catatcgaag gtggtaaagt aaaagttggg
     1081 attcacgaac tccggcacgg gatcgatcaa ctccgttgct accgcttttc ccaccaccgc
     1141 cgccagcaga atgggtacag catcaagtga tgtgaacttc ttgctgatca cctccatgct
     1201 gaatatcgcc ccgcccattg gtgcattgaa ggtggcggca atccccgagg ccacgccgca
     1261 gcaggtgagc acctttaaat cgcgtgcaga tagctttatg gcttttccga tagccgagcc
     1321 cgccgaggct ccaatcaggg caatgggccc ctcccgcccg gcgctgcccc cgctgcccag
     1381 ggttattgcg gaggtgaaga tgagaaccag accggctcgc tttctgatgt cgccggtaaa
     1441 cctctgcaga gcgatcatga tatgggatat gccgtcgccc ttcgcctccg gcgccagctt
     1501 gtagatcaga ggtccgacta tgaggccgcc aaggaccggc agcagaacga cgaagtagct
     1561 attcggcagg agagggagga gaaagccgaa gaagaaatcg tggctaagct ccaccagctt
     1621 tcggaaggcc actgccccca ggcccccgct tatccctacc acaatagaga gaaggtccag
     1681 gtagatccat cgcagatctt ctgtgagctt cagatgggta ttcatccact cgccatgggc
     1741 taacacatat tcagatggtg ataaaaaagc ttttcttttg gaggtgacta ctccgaactt
     1801 aaggaaggct tgatcagagg gatggaatgg atttgaattt atcgattatc ttcgttcttt
     1861 tggctgcgag tgctgcaatc tcgactgctg ctccgggcgg agaatcaatg attgctgatc
     1921 tgaccatgat tcatgccat
//
LOCUS       DAOSGP010000267         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_60690_length_1911_cov_16.930496, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000267 DAOSGP010000000
VERSION     DAOSGP010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60690_length_1911_cov_16.930496"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1070
                     /gene="cofH"
                     /locus_tag="PLV49_06735"
     CDS             33..1070
                     /gene="cofH"
                     /locus_tag="PLV49_06735"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HOG98575.1"
                     /translation="MERACLSPKSPGNDHDLLMRLWFEPLRLFRLANLLRQKSGKDEV
                     TFIINRNINFTNQCIGSCKFCSFKHSKRYRLSKEEILSRAREAEERGATEICLQGGLS
                     PGMLLEDYCGILEIIHRSHPRLHLHAYSPMEVLHMSRNSNVPVKDCLKELKRSGLGSM
                     PGTAAEILVDSVRQKICPEKLNAGQWKEIIVAAHHLGIPSTSTILFGHVESIEDRLEH
                     LEILKGIQNETGGLTELVLLPFIPENNLLGRTVPGADLLDRLKMHALARVALYPAVTN
                     IQASWTKLGRDAAKAAIMWGANDLGGTLMDERIARNSREEPGISAEEIKELIESCGRK
                     AVQRTTLYERI"
     gene            complement(1123..>1911)
                     /locus_tag="PLV49_06740"
     CDS             complement(1123..>1911)
                     /locus_tag="PLV49_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98576.1"
                     /translation="SRSWDWHVRRKLAWNSDTHVEILSYLARDQVQWVREAVAGNACT
                     PSSTLARLAEDSSGFVRAAVALNRQATSEMLESLAGDEMTDYDSTLQKNRYLVKEAVA
                     KSPNINPQTLEFLARDSDEHVRACAASNPLLTAELMKRMVKDVSWLVRNNIARNPSTP
                     EELLAYLTLDRIMDVRATAALNPRTPAESLAALARDRSWEIREATARNPTIDGKLLDE
                     LASHWSWMVREAVAKNPNTLAETLERLAADPDQSVQKAARSRAD"
BASE COUNT          411 a          536 c          556 g          408 t
ORIGIN      
        1 gagaatcgtt gaccgtctca gatctctcgg agatggaaag agcctgcctc tctccaaaaa
       61 gtcccggcaa tgatcatgac ctgctgatga ggctctggtt tgagcctctt aggcttttcc
      121 gcctggccaa tctattgcgt cagaagagcg gcaaggacga ggtcactttt ataataaacc
      181 ggaacatcaa cttcactaac cagtgcatag gaagctgcaa attctgctct tttaagcata
      241 gcaagagata ccgtctttca aaagaggaga tcctcagccg ggcgagggag gcagaggagc
      301 ggggagctac agagatctgc ctacagggag gactctctcc cggcatgctc ctggaggact
      361 actgcggcat cctggagatc atccaccgca gccatccccg gttgcatctt catgcctact
      421 ctcccatgga ggtgctgcat atgtctcgca actccaatgt gccggtgaaa gactgcttaa
      481 aggagctgaa gaggagtggt ctgggatcaa tgcccggcac cgcagctgag atcctggtcg
      541 attccgttcg ccagaagatc tgcccggaga agctgaatgc agggcagtgg aaggagatca
      601 tagtggccgc tcatcatctt gggatcccct ccacctccac cattctcttc ggccatgtgg
      661 agagcataga agatcggctg gagcacctgg agatcctgaa gggaattcaa aatgagaccg
      721 gagggctcac ggagctggtc cttctcccct tcatcccgga gaataatctg ctgggaagga
      781 ctgttcccgg agcagatctc ctcgatcggc tgaagatgca tgccctggcc agggtcgccc
      841 tctatcctgc cgtcaccaac atccaggcta gctggaccaa actggggcgg gatgcggcaa
      901 aagctgccat catgtggggg gcaaacgacc tgggggggac gcttatggac gagagaatcg
      961 cccgcaactc cagggaggag ccaggaatct cggcggagga gatcaaagag ctgatcgaga
     1021 gctgtggacg gaaagctgtg cagaggacca ctctgtatga gcggatttga catccgataa
     1081 agctggcagg tttcgaatgg tggtggagga aaaaacgctc cgtcagtctg cccgacttct
     1141 ggccgccttc tgcacgctct ggtcgggatc tgcggccaat ctctcgagcg tctcagccag
     1201 agtattggga ttttttgcca ccgcttccct caccatccag gaccagtggc tcgcgagctc
     1261 atccaggagc tttccatcaa tcgtgggatt gcgggctgtt gcctctctga tctcccaact
     1321 cctgtccctg gcaagagcgg ccagcgactc tgcgggagtg cggggattga gggcggcagt
     1381 cgctctcacg tccatgatcc tgtccaaagt taagtaggcc agtagctcct ctggagtgga
     1441 aggatttcgg gcgatattgt ttctcaccag ccagctcaca tccttgacca tcctcttcat
     1501 cagctcagcc gtgagcagag gattggatgc cgcgcaagcc cggacatgct catcggagtc
     1561 gcgggcgagg aattccaggg tctgggggtt gatattgggg ctttttgcca ctgcctcctt
     1621 tacaagatag cgattcttct gcagagtgga gtcgtagtca gtcatctcgt caccggccag
     1681 agactccagc atctcgcttg tggcctggcg gttcagggcc acagccgctc tcacaaatcc
     1741 cgagctgtcc tctgccagtc gggccagggt cgaggacggg gtgcaggcat tgcccgctac
     1801 tgcctctctc acccactgga cctggtcccg ggcgagatag ctcaggatct ccacatgggt
     1861 atcggaattc caggccagct ttcgtctcac atgccagtcc caggagcgag a
//
LOCUS       DAOSGP010000268         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_60691_length_1911_cov_16.289871, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000268 DAOSGP010000000
VERSION     DAOSGP010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60691_length_1911_cov_16.289871"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLV49_06745"
     CDS             <1..464
                     /locus_tag="PLV49_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG98577.1"
                     /translation="KIDEQIIDQLKEAALRSPSSRGVNPWRLLFITDKDMLEVLSRAK
                     QSGSGFLKGASLGVVVIARNEESDVWIEDCSIASIILQLTGQSLGLGSCWIQIRNRKH
                     NDSQTSEDYIRKALDLPEGSKVECMIAFGYPDEIKQPHPKSELEYGKILES"
     gene            640..1218
                     /locus_tag="PLV49_06750"
     CDS             640..1218
                     /locus_tag="PLV49_06750"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOG98578.1"
                     /translation="MDSVVNMAMEQLMAGDNLSILSSKVGADEQSTKSALEMGLPLLL
                     GAMSSKASSNDGANAILSGLADSAKGNSMQDMAKYFGSSSSSFGPGMLNSILGSSMIP
                     IQQSIAKKTGLPQEVVGQLLTMALPLVLGYLTKGSSEKEMKPDDLSKLLDEQSKMALT
                     SSPEAEAMMKDILSAQDSGSGFMGMIKKLFKS"
     gene            1617..>1911
                     /locus_tag="PLV49_06755"
     CDS             1617..>1911
                     /locus_tag="PLV49_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98579.1"
                     /translation="MYIISVLILLAIACSSQSTDTVNISRDSGMAILANLTESSSSPS
                     EAQESILQNQTNQTNQTGNTSLLANDDLWSWGKIPIGYEVSDNGTLINLAEQEW"
BASE COUNT          538 a          424 c          514 g          435 t
ORIGIN      
        1 tgaagatcga tgaacagatc attgatcagc tcaaggaggc tgctttgcgc tcacccagct
       61 ccaggggggt caatccctgg cggctcttgt tcatcaccga taaggatatg ctggaggtgc
      121 tatctcgcgc caaacaaagc ggctcgggat tcctgaaggg agccagtctg ggtgtggtgg
      181 tcatagccag aaacgaggaa tcggacgtct ggattgagga ctgctcaatc gcttccatca
      241 tcctgcagct gactggccag agcctggggc tgggatcatg ctggatccag atcagaaacc
      301 ggaagcataa tgatagccag acatctgagg attatatccg aaaggccctc gatctgccgg
      361 agggatcgaa ggtggaatgc atgatcgcct ttggctaccc tgatgagata aagcagcccc
      421 atcccaagag cgagttggag tacgggaaga tactggaatc ataggaagga gtagttggga
      481 aaagggcatg aggatggtcc taacccctca ctctttttta ttctgacaat tattaactct
      541 ggttagaact actgaatgtc cagagaaagg agttaatact cattagagca tatatgctta
      601 gcaagtacat agctcaactg ttcattagga gatgaattaa tggattcagt ggttaatatg
      661 gcaatggaac agcttatggc gggagataat ctctctatat taagcagcaa agttggtgca
      721 gacgaacaat ccaccaaatc cgccctggag atgggcctgc ccctattatt gggagccatg
      781 agcagcaaag cctccagcaa tgatggggcg aacgctatct tgagcggtct tgccgattca
      841 gccaagggca actcaatgca ggacatggcc aaatactttg gcagcagcag ctcatccttt
      901 ggaccgggca tgctcaattc catattgggg agcagcatga ttcccattca gcaatcgatc
      961 gctaaaaaga caggactgcc tcaggaggtc gtcggccagc tgctgactat ggctctgcct
     1021 ctggttctgg gatacttgac aaaaggctcc tcagaaaagg agatgaaacc ggatgatctc
     1081 tcaaagctgc ttgatgagca atcgaagatg gcactcacct cgtcgccaga ggctgaagca
     1141 atgatgaagg atatcctatc cgcacaggac agcggcagtg gatttatggg gatgatcaag
     1201 aagctgttta aatcttagcc atatctctcc cgagtgggga aggctgctcc tgcggcagca
     1261 gccatgcaga tgagcgaaaa tagccacaaa tttttgatag gattccggct ctggtggtct
     1321 gcacgaggct ggatagctgc aggactgttt tgtcagtgaa gccaaatatg ttcaggggca
     1381 gccttgctcc tccccacaat gctgatttgg agactgctcc ctggctgcag aaatgtccag
     1441 aattcatatc gatgcaaaaa ttgatatgtt ggtgaacata gaaagaaaga ggaaggaata
     1501 agctgataga tgagggcgac tgcagcatct aaaaaaaagc cgttggtcga atcctcctat
     1561 caaggatatc attggagagt cttggtataa cgccatagga ggttatattt atgaagatgt
     1621 atataatttc tgtattgatt cttttagcga tagcatgcag cagccagtcg acggatacag
     1681 tgaacatcag cagagattct ggcatggcaa tactggccaa tctgacagaa agctcttcca
     1741 gcccctccga ggcccaggag agcatactgc aaaaccagac aaaccagaca aatcagactg
     1801 gcaacaccag tctgttagcg aatgatgact tgtggagctg gggcaagatt cccattggct
     1861 atgaggtaag cgataacgga actctgatca acctggccga gcaggaatgg g
//
LOCUS       DAOSGP010000269         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_60870_length_1907_cov_16.834233, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000269 DAOSGP010000000
VERSION     DAOSGP010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60870_length_1907_cov_16.834233"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLV49_06760"
     CDS             <1..189
                     /locus_tag="PLV49_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98580.1"
                     /translation="EVVRFISQEISVHTYLNVMAQYHPEYQACRYPELSRAITAREYA
                     QALGLAQRAGLVRGLAML"
     gene            215..643
                     /locus_tag="PLV49_06765"
     CDS             215..643
                     /locus_tag="PLV49_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOG98581.1"
                     /translation="MIPFEYLEHTADVKFRAYGTTPDQMLSNAAAALFGAMISPCQVK
                     AKELWQVELEAEDLEDLAYQWLSEIVFLFETESAVFSRFQVALEEVQDGKGWKLRAEI
                     GGERMDPERHSFLAEVKAVTRHKFAIKKNERWCIQVVLDV"
     gene            696..>1907
                     /locus_tag="PLV49_06770"
     CDS             696..>1907
                     /locus_tag="PLV49_06770"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOG98582.1"
                     /translation="MEIEKIDDNIYEVPIGYRPGMRVPGRIFVSAALREMVEPETVDQ
                     VANVATLPGIVGHSMAMPDAHLGYGFPIGGVAAFKEQGGIISPGGVGFDINCGVRLLR
                     SDLQARDVQPLINTLLDALFQAVPSGLGSTGRLHATDQQLTESFLRGAEWAVEEGYGV
                     EADLDFCEENGSMPGADPSQVGVKARKRGRPQLGTLGSGNHFLEIQRVEEIYDQEIAK
                     RFGLFKDQITVMIHCGSRGAGHQICTDHLDVLSKAVRKYNIDIPDKQLACAPLGTPEA
                     DHYFGAMVCAANYAWANRHIIAHWTREVFDRYFPGSQLQLLYDVAHNVAKIEEHRVEG
                     KKERLYVHRKGATRAFGPSRTEIPAAYEGLGQPVLIPGSMGTASYVLCGQDKALDLTF
                     GSACHGSGRVMS"
BASE COUNT          381 a          558 c          596 g          372 t
ORIGIN      
        1 gaggtggtgc gcttcatctc ccaggagata tccgtgcaca cctatctgaa tgtcatggcc
       61 cagtaccatc cggagtacca ggcctgccgc taccccgagc tttcccgcgc cataaccgcc
      121 cgcgagtacg cccaggctct cggccttgcc cagagggcag gcctggtccg cggactggca
      181 atgctttaat gcctgcggct ctcactaata tatgatgatt ccattcgaat atctggagca
      241 taccgcagat gtcaagttcc gcgcctatgg cacgactcct gaccagatgc tctccaatgc
      301 cgcagcagcc ctatttggcg ccatgatctc cccctgccag gtgaaggcga aggagttatg
      361 gcaagtggag cttgaggcgg aggatctgga ggacctggcc taccagtggc tctctgagat
      421 cgtctttctc ttcgagaccg agtctgcggt cttctcccgc ttccaggtgg cgctggaaga
      481 ggttcaagat ggcaagggat ggaagctccg ggcggagatc ggcggggaga ggatggaccc
      541 ggagaggcac tcgttcctcg ctgaggtgaa agcggtcacc aggcacaagt tcgccatcaa
      601 aaagaacgag cggtggtgca ttcaggtggt gcttgatgtc tgatggctct atgtctgatg
      661 atgttctatg tgtgataatt gtggacgtgt gatatatgga aattgagaag atcgatgata
      721 acatctacga ggtgcccatc ggctaccggc cgggaatgcg ggtgccgggc aggatattcg
      781 tctcagccgc cttgcgagag atggtcgagc cggagacggt cgaccaggtg gccaatgtcg
      841 ccacccttcc cggaatcgtc ggacactcca tggccatgcc cgatgcccac ctgggctacg
      901 gctttcccat cggcggggtg gccgccttca aagagcaggg aggtatcata agcccaggag
      961 gcgtcggctt tgacatcaac tgcggggttc gcctgctccg ctcggacctg caggccaggg
     1021 acgtgcagcc gctcatcaac accctcctcg atgccctctt tcaggcggtg ccttcgggct
     1081 tgggatccac aggcaggctg catgccaccg accagcaact gaccgaatcc tttctccgcg
     1141 gagcggaatg ggcagtcgag gagggctatg gagtggaagc ggacctggat ttctgcgagg
     1201 agaatggctc catgcctgga gcggacccct cccaggtggg ggtcaaagcg aggaagcggg
     1261 gccgccccca gctggggacc ctgggcagcg gcaaccattt cctggagatt cagagggtcg
     1321 aggagatcta tgaccaggag attgcaaaaa ggttcgggct tttcaaagac cagatcacag
     1381 tgatgatcca ctgcggctcc cgtggggcgg gccaccagat ctgcaccgat cacctggacg
     1441 tcctgtccaa agcggtgaga aagtacaata tcgacatccc tgataagcag ctggcctgcg
     1501 ctcctctggg cactcccgag gcagatcact acttcggggc catggtctgc gccgccaact
     1561 atgcctgggc caaccggcat atcatcgccc actggacgag agaggtcttc gaccgctact
     1621 tccccggctc ccagctgcag ctgctctacg atgtggccca taatgtggcc aagatcgagg
     1681 agcatcgggt ggagggcaag aaggagaggc tctatgtcca ccgcaagggg gccacccgtg
     1741 ccttcggtcc ctcaagaacg gagattcccg ctgcttatga gggcctgggc cagccggtgc
     1801 tgataccggg gagcatgggc acggcctcat acgttctctg cggccaggac aaggcccttg
     1861 atctgacctt cggctccgcc tgccacggct caggccgggt gatgagc
//
LOCUS       DAOSGP010000270         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_60969_length_1905_cov_11.549189, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000270 DAOSGP010000000
VERSION     DAOSGP010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60969_length_1905_cov_11.549189"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLV49_06775"
     CDS             complement(<1..266)
                     /locus_tag="PLV49_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98583.1"
                     /translation="MFAMIELEIRRLRHDRTEIYTRAVQPILWLGVFGTIMGRVNAIP
                     TGGLPYIDYITPGVLLQSTIFVSVFYGDKGPDDQRGAGQHSGRYA"
     gene            complement(299..1282)
                     /locus_tag="PLV49_06780"
     CDS             complement(299..1282)
                     /locus_tag="PLV49_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG98584.1"
                     /translation="MTAGIDVRGLSKLYNGKIRALDGVDLRVEAARVFAFLGPNGSGK
                     TTLMRILTTQIRPTSGQACIFGLDAIRNGQEIRKIIGYVPQETSVWLDISGYENLLVY
                     SKIYGVPAGMRQKRIQDALQSMGIEEMADRMVKSYSGGMVRRLEIACALLVGPKILFL
                     DEPTIGLDPSARKAVWENLISFKKEYGTTVFFNTHYMDEADLYSDEIAIINKGSIVKS
                     GTASELKQSLRSEIIQVYPHGRIDGQVLKSIRDLAFVRGAINNNSHLEIVVEDSETKL
                     PLILDVLRNEGVSLKRICTAKPTLDDVFLKYAGAVRFKGKGEDPVEKALQA"
     gene            1617..>1905
                     /locus_tag="PLV49_06785"
     CDS             1617..>1905
                     /locus_tag="PLV49_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98585.1"
                     /translation="MKMKYQTITMVLLLALVPGLAMADNALMEALGGKAAQKAMQDLG
                     FEKGDANVLVLTDAGHAIVDGQTSQAAIKGITNESGNSIGDGNLFRPLRAHW"
BASE COUNT          473 a          497 c          423 g          512 t
ORIGIN      
        1 gcatatctac cagaatgttg cccagctccc cgctgatcat caggcccctt atcgccatag
       61 aatacagaga cgaatatggt ggactggagc aatactcccg gagtgatgta atcgatatag
      121 ggcagcccgc cggtgggtat ggcattgacc cggcccataa tcgttccgaa gacgcccagc
      181 cacagtatgg gctgcaccgc tcgggtatag atctcggtgc ggtcatgcct gagccttctg
      241 atctccagct caatcatggc aaacatctcc ctgatcgaat catctggagc catattaatc
      301 atgcctgcag tgctttttcg accgggtcct ctcccttgcc tttgaaacgg actgcaccgg
      361 cgtatttgag gaatacgtca tccaatgtag gcttggcagt gcagattctc ttcagggata
      421 cgccctcgtt cctcaagaca tccaggatca aaggcagttt ggtctccgag tcctcgacca
      481 ctatctccag gtgggagtta ttattgatcg caccccggac aaatgctaga tccctgatgc
      541 tcttcagaac ctgcccatcg atcctgccat gtggataaac ctgaatgatc tcgctcctta
      601 aagattgctt gagctcgctt gccgttccgg atttcacaat gctgcccttg tttattatgg
      661 ctatctcatc ggagtagagg tctgcctcat ccatataatg agtattgaaa aatactgtag
      721 tgccatactc cttcttgaat gagatcaggt tctcccagac tgcctttctt gccgacgggt
      781 ccaggcctat agtcggctca tccagaaata gaatcttggg cccaaccaga agggcgcagg
      841 cgatctccag ccgccgcacc atccctccgg agtagctctt gaccatcctg tccgccatct
      901 cctcgatgcc catgctctgc agtgcatcct gtatcctctt ctgcctcatt ccagctggaa
      961 cgccatagat cttggaatag accagcagat tctcgtatcc gctgatatcc agccagacgc
     1021 tggtctcctg aggcacatag ccgatgatct ttcttatttc ttgaccattc cgaatcgcat
     1081 ccaagccgaa gatgcatgct tggcccgagg tcggcctgat ttgggtggtc agtatcctca
     1141 tcaaggtggt cttgccagag ccatttggac cgagaaaggc gaagactctg gctgcctcta
     1201 ccctcagatc gaccccgtcc agggctctga tcttgccgtt ataaagcttc gagagccctc
     1261 ttacatcaat tccagctgtc acttcatctt catctctctc ttcttttcaa cctcttttga
     1321 acattccatt tttttacaat ttattacaat tatttgttat cgtaattgct tcactacaat
     1381 agatcaggga ttagatttaa tctttattaa cttttctgaa gaaaatagat ttaaatatgg
     1441 aaatgatatc aaatacgtat caacaaataa tcgtaggtta tgtgatttgt ttagccaata
     1501 cttgcacgag ctccatgaag gatagacaat acatagtatt acgctattta tgctacaaat
     1561 ttgaagaaaa atcttaaata gactgatgtc aattgcataa gatcagagga attcgtatga
     1621 aaatgaagta tcaaaccata accatggtcc ttctcctggc tctggttccg ggattggcca
     1681 tggcggacaa tgcattaatg gaagcgctgg gaggcaaagc cgcccagaaa gcaatgcagg
     1741 atctgggatt cgagaaggga gatgctaatg tcctggtttt aaccgatgcc gggcatgcca
     1801 tcgtcgatgg ccagacatct caggctgcga tcaagggaat cacaaatgaa agcggaaaca
     1861 gcatcggcga tggaaacctt ttcaggcctc tgcgcgctca ctgga
//
LOCUS       DAOSGP010000271         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_61500_length_1894_cov_15.613921, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000271 DAOSGP010000000
VERSION     DAOSGP010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61500_length_1894_cov_15.613921"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLV49_06790"
     CDS             <1..699
                     /locus_tag="PLV49_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG98586.1"
                     /translation="EGNVGSVARAMKNFGFHDLVLVNPCKIEDYGCAMASHARDVLQM
                     SRNFSTLAEALEGANLVVGTTGKRLEHEQHHLRLHLRVPCLTPAGLAEKLRDKEGTVA
                     LLLGREDCGLNSDEISLCDMIVSIPTSDEYPVMNLSHSAAVLFYELSRNGSNSSGNVQ
                     MARKETLNLLQKRAGDLLADIAYPEHKEDYTLLMLRRIFGRAELTEREARALLGIIKR
                     ILWRIGHPPGSDRD"
     gene            complement(762..1820)
                     /locus_tag="PLV49_06795"
     CDS             complement(762..1820)
                     /locus_tag="PLV49_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98587.1"
                     /translation="MNCIYIVLVDVMNHSSAVIVLMALLFLSTSGEALRTFSGDTISI
                     VNPVEDDIFAAGNIVNINAPVDSAVVAGGTVNINAPVKGDVIAAGGQVYVNSDVGGKV
                     VAVGGTIDLRGDVGTNLVAAGGQVSILPGRTVGRDALISAGNAVNSGRINGTMTVYAS
                     DFNNTGSAGKVDFYQTERTEDKAPPREDKWGGFDLFSLISIIGYFILGLILVKHIPVI
                     FRTVDEEIRTSTLLRTVLGFIIIIASFIALLLVAITVVGLPIAVVSTFLVIVALMLSG
                     TFVAYSLGRWICLQGKLKQGDLVCFTIGFVILNLLFLIPYLGGLVSIVSMSLGFAALL
                     YAARRLIMASNDTAAAME"
BASE COUNT          441 a          530 c          528 g          395 t
ORIGIN      
        1 gagggcaatg tgggctctgt ggcccgggcg atgaagaact tcggctttca tgacctggtc
       61 ctggtcaacc cctgcaagat cgaggactat ggctgtgcca tggcctctca tgcccgggac
      121 gttctgcaga tgtcccgcaa cttctccact ctggccgagg ccctggaggg ggcgaacctg
      181 gtggtgggca caaccggcaa gaggctggag catgagcagc accacctgcg gctgcaccta
      241 agggtgccct gcctgacccc ggccggtctg gcagaaaagc tccgggataa ggagggaacg
      301 gtcgccctgc tcctggggag ggaggactgc ggcctgaaca gcgatgagat ctccctgtgc
      361 gatatgatcg tctccattcc cacctcggat gagtacccgg tgatgaacct ctcccactct
      421 gcagcggtgc ttttctatga gctctcccgc aacggaagca atagctcggg caatgtccag
      481 atggccagaa aagagacact gaacctattg cagaagaggg cgggagatct ccttgcggat
      541 atcgcatatc ccgagcataa ggaggactat accctgctga tgctgcggcg catcttcggc
      601 cgggcggagc tgacggagag agaggctcga gcgcttctgg ggatcataaa gaggatcctc
      661 tggaggatag gccatccccc cggctcggat cgagattgaa aaaataattc tggcaggccg
      721 aagtaggggt gccaggaaaa aaagttgatc tctcctttct tctattccat cgctgccgcc
      781 gtatcgttgc ttgccataat caggcgccgg gcggcgtaca gaagggcagc gaagcccagg
      841 ctcatggata caatggacac caggccgccc aggtagggga tgaggaatag aagattgagg
      901 atgacaaatc caatggtaaa gcataccaga tccccctgtt ttagcttgcc ctgcaggcag
      961 atccaccggc ccaggctata ggccacaaat gtcccgctga gcatcagggc gacgatcacg
     1021 aggaacgtag ataccactgc tatcggcagg cccaccacgg tgatggccac cagcaagaga
     1081 gcgatgaatg aggcgatgat gatgatgaat cccagtacgg tcctgagcag ggtggaggtg
     1141 cgaatctcct cgtccaccgt ccggaagatg acgggaatat gctttaccag gatcagtccc
     1201 aggatgaagt agccgatgat gcttatcagg ctgaagagat caaagccgcc ccatttatcc
     1261 tctcttggcg gagccttatc ctctgtcctc tccgtctgat agaagtcgac cttgcctgcc
     1321 gagccggtgt tgttgaagtc gctcgcataa acggtcattg tgccattgat ccggccggag
     1381 ttcaccgcat tgccggcgct gatgagggca tctctgccca ctgtcctgcc cggaagtatg
     1441 ctcacctggc ctcctgctgc caccagattg gtgccgacat ctcctctcaa atcgatggta
     1501 cccccgacag ccaccacctt tccgcccaca tcggagttca catagacctg gccgccagca
     1561 gcaatgacgt ctccctttac tggcgcgttg atgttcactg tgcctccggc gactacagcc
     1621 gaatcaactg gagcgttgat attcactatg tttcctgcgg caaagatatc atcctctacg
     1681 ggattgacta tggatatcgt atcgccagaa aaggtcctca atgcctcccc actggtggag
     1741 aggaataaaa gggccatcag aactattact gcactggaat gattcatcac atccaccaga
     1801 actatgtaaa tgcaattcat cagataaata acccacttaa gatgatcatt gaggctcctc
     1861 ttttacggta aggatagggc ttatcaactc aggg
//
LOCUS       DAOSGP010000272         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_61909_length_1887_cov_20.313319, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000272 DAOSGP010000000
VERSION     DAOSGP010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61909_length_1887_cov_20.313319"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLV49_06800"
     CDS             <1..744
                     /locus_tag="PLV49_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOG98588.1"
                     /translation="FFLAYSPERIMTGYSISRLREFPKVIGGTDEESGLAAYQLYRQF
                     ISNLHLVSSASVAEMIKVMEGCYRDVNIALANELYKICQDLGIDFFEAREKACHQFCH
                     IHLPSTGVGGHCIPVYPWFLIKEMEKREMFDKCRILHTSRILNDEMIQYWAERIMLEC
                     MKINKPLCEIKICIKGITFRAGVREFYHSRNLALARLLYDKGLDVYVADPLLNNDEVA
                     ARGFESIKPQEADLVFDPFQLNFESGKAL"
     gene            757..1833
                     /gene="wecB"
                     /locus_tag="PLV49_06805"
     CDS             757..1833
                     /gene="wecB"
                     /locus_tag="PLV49_06805"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOG98589.1"
                     /translation="MKIATIVGARPNFIKLAPVSKEIRKRDHKEVIVHTGQHYNYEMD
                     RIFFDQMGIAAPDYHLGIGSGSHGFQTGEMLKKTEEVLQKEMPDAVIVFGDTNSTLAG
                     GLAAAKMHIKCAHIEAGLRSFDKRMPEEINRVLVDHCSDLLLCPTRTAIDNLKMEGIV
                     QNVHLTGDVMVDAQKDCERIAESESHILEALGLKPRSYYLATVHRASNTDDPENLRAI
                     ALALQDLDDVIFPCHPRAEKYLKKFGLWDDLEKKIRMIKPVGYLDMLLLEKNAKKIVT
                     DSGGVQKEAYLLGIPCITLRDTTEWIETVNDGWNVLVRPGEDIASAIRDFRPRHERQD
                     VFGKGRASAMIVDLVEKLVGSGGS"
BASE COUNT          480 a          455 c          555 g          397 t
ORIGIN      
        1 ttcttcctgg cctactcccc ggagaggata atgaccggat atagcataag ccggctgagg
       61 gagtttccca aggtgattgg tggcacggac gaggagagcg gcctggcagc atatcagctc
      121 taccggcagt tcatctccaa cctccatctg gtctcatcgg ccagtgtggc ggagatgatc
      181 aaggtgatgg agggctgcta cagggatgtg aacatcgccc tggccaatga gctctataag
      241 atctgtcagg atctgggaat cgactttttc gaggcgagag agaaggcttg ccatcagttc
      301 tgccatatcc acctgccttc caccggggtg ggagggcact gcatccccgt ttacccctgg
      361 tttttgatca aagagatgga aaagcgagag atgttcgaca aatgccgcat tcttcatacc
      421 tcccgcatcc tcaatgatga gatgatccag tactgggcgg agaggataat gctggagtgc
      481 atgaagatca acaaaccgct ctgtgaaatc aagatctgca tcaagggcat aacattccgg
      541 gcgggggtca gagagttcta tcacagcaga aaccttgcac tggcaaggct tctttatgat
      601 aaagggctgg acgtctatgt ggccgatcca ctgctgaaca atgatgaagt ggccgccagg
      661 ggctttgaaa gcatcaagcc ccaagaggcc gacctggtat tcgatccctt ccagctgaac
      721 ttcgaatctg ggaaagctct ctagggactt gatcgcatga agatagcaac cattgtcggc
      781 gcccggccca acttcatcaa gctggctccg gtatcaaagg agataagaaa gcgagaccat
      841 aaggaggtca tagtccatac cggccagcac tataactatg agatggacag gatattcttc
      901 gatcagatgg ggatagctgc tccagactat cacctgggca taggctcggg cagccatgga
      961 tttcagaccg gtgagatgct caagaaaacg gaagaggtcc tgcaaaagga gatgccggat
     1021 gcggtcatag tattcggaga caccaactcc acactagccg gtgggctggc ggcggcaaag
     1081 atgcatatca agtgtgctca tatcgaggcc ggactgaggt cgtttgacaa gaggatgccc
     1141 gaagagatca accgggtgct ggtggaccat tgctctgatc tcctcctctg tccgaccagg
     1201 actgcgatcg ataacctgaa aatggaaggg attgtccaga acgtccattt gacgggagat
     1261 gtgatggtgg acgcgcaaaa ggattgcgag aggattgccg agagcgaatc tcatatcctt
     1321 gaggctctgg ggctcaagcc ccggagctac tatctggcaa ctgttcaccg ggcatccaac
     1381 accgatgatc cggaaaacct cagggcgatt gctctggccc tccaggatct ggatgatgtg
     1441 atctttccct gccatcctcg cgcggagaag tacctgaaga agttcggcct gtgggatgat
     1501 ctggaaaaaa agatcaggat gataaagcca gtgggatacc tggatatgct tcttttggag
     1561 aagaacgcca aaaagatcgt caccgactcg ggtggggtgc aaaaggaggc atatctcttg
     1621 ggcattccct gcataaccct tagggacacg acggaatgga tagagacggt aaacgacggc
     1681 tggaacgtcc tcgtccggcc gggggaggat atcgcctctg cgatcagaga tttccggccg
     1741 aggcatgagc gccaggatgt atttggaaag ggcagggcga gcgctatgat cgtggatcta
     1801 gtcgagaagc tggtcgggtc aggtggatct tgaagatact ccttttagcc aatcagccgg
     1861 agaggaccac caggctcagg atgttca
//
LOCUS       DAOSGP010000273         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_64138_length_1844_cov_18.403018, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000273 DAOSGP010000000
VERSION     DAOSGP010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64138_length_1844_cov_18.403018"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLV49_06810"
     CDS             complement(<1..542)
                     /locus_tag="PLV49_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HOG98590.1"
                     /translation="MSGIDSDILREKIRERMPKPPHFSLGATAFIATAIILLLLLVLV
                     GGSFIAIIPAGHVGVQDRFGVVSDTVLSPGFNLKDPLTSVHQMNTQTQQIEYKQVTGT
                     LTREGLEINLDSSVLWHLDPAKAPDIFRSVRGDYVDTKLTPSFMGLLRAEIKKYTAED
                     IYTNKSTEIQADVEKQLKMEL"
     gene            complement(623..1519)
                     /locus_tag="PLV49_06815"
     CDS             complement(623..1519)
                     /locus_tag="PLV49_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98591.1"
                     /translation="MNEIREISSLLDRELEAIKHSFSNDLASEKIGGKASSRVVKFLN
                     PKVITSENKIEVMGPAFLISLAELKKAIKNRYKTPGRNIFPEVADDFVFKNGMDAINR
                     LSNFDTSQAASDLVNLSRLVKVPSDSEKVALVRRIKPLELVDEYIIDGKHTSGSFLGS
                     FDLPLVVMTDDGAQFDFENPSDVMGTDKRLEESMSINMKSIMLIAERVDDNTIQTTSG
                     LKHVFLGNTMAVISLICLLIGAFSLIERRLFFFIILFICGVALAVARYLPTSWNREFF
                     GVSEMKVVGNGYTVFKFYGDMT"
     gene            complement(1516..>1844)
                     /locus_tag="PLV49_06820"
     CDS             complement(1516..>1844)
                     /locus_tag="PLV49_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98592.1"
                     /translation="QSRQLTCCNIDNPEDLEVSMDSCEMLFLPARDPGVLYLKEGMCS
                     VSGPKRSDLAREIDRPARLLDELPRRDPDVVRMDERTFRQTGTISIQSPFTLVILDGQ
                     PGAAKL"
BASE COUNT          405 a          526 c          437 g          476 t
ORIGIN      
        1 agctccatct tgagctgctt ttccacatcc gcctggatct cagtcgactt attggtgtaa
       61 atgtcctcgg cggtgtactt cttgatctcc gctcgaagca ggcccatgaa ggaaggagtc
      121 agcttggtgt ccacatagtc tcctcttacc gatctgaaga tgtccggagc cttggccgga
      181 tcaaggtgcc acaggacgct gctgtccaga tttatctcca ggccctcacg ggtcaacgtc
      241 ccggttacct gcttgtactc tatctgctgg gtctgggtgt tcatctggtg gacgctggtg
      301 agtggatctt tgaggttgaa tcccggactc aggacagtat cggagaccac cccaaaccgg
      361 tcctgcaccc ccacatgccc ggcagggatt atggctatga aggatcctcc cactaaaacc
      421 agcagcagca ataagatgat tgcagtggca atgaaagctg ttgctccgag agagaaatgt
      481 ggtggcttag gcatcctctc tcttattttt tcccgtaata tatcgctatc tattccgctc
      541 atctattcct ccgctatata ctgctatgct cattagcttt gctttgctct ttattggtga
      601 gatatttctc atcccaggaa catcatgtca tatctccata gaacttgaag accgtgtagc
      661 cattgcccac caccttcatc tccgagacgc caaagaactc tctgttccag ctggtgggca
      721 gatatctggc caccgccaga gccaccccac agatgaacag tataatgaaa aagaacagcc
      781 tgcgctcgat tagcgagaag gcgcctatta aaagacagat cagggatata actgccattg
      841 tgtttcccaa aaatacatgc ttcagcccgc tggtggtctg aatggtgttg tcgtccaccc
      901 gctcggctat gagcattatg ctcttcatgt tgatggacat cgactcttcc agccgcttat
      961 ctgtgcccat cacatctgag ggattctcaa aatcaaactg ggcaccatcg tcggtcataa
     1021 ccaccagggg aagatcaaag ctccccagga agctgccgct ggtatgcttg ccatcgatta
     1081 tgtactcgtc caccagctcc agcggcttta tccttctgac aagagcgacc ttttccgagt
     1141 cagaaggcac tttcacaagc cttgacagat tgaccagatc gcttgccgcc tggctggtgt
     1201 cgaagttgct cagcctgttt atggcatcca tgccgttctt gaatacgaag tcgtcggcca
     1261 cctccgggaa tatattccgc cccggcgtct tgtagcggtt tttaattgcc ttcttcagtt
     1321 cggccaggct tatcaggaac gccggcccca taacctctat cttattctca gaggtgatga
     1381 ccttgggatt cagaaatttt accaccctgg aagaagcctt cccgcctatc ttctcagaag
     1441 ctagatcatt ggagaaagag tgctttatcg cctctagctc tcggtccaag aggctggaaa
     1501 tctccctgat ttcgttcata gcttggccgc tccgggctga ccatccagga tcaccagagt
     1561 gaaaggactc tggatagaga tcgtccccgt ctggcggaat gtcctctcgt ccatcctcac
     1621 cacatccgga tcccttcttg gcagctcatc cagaagcctg gccgggcgat caatctcccg
     1681 ggccagatcc gagcgcttcg gcccgctgac agagcacatt ccctccttca gatagaggac
     1741 gcccggatct cttgccggca aaaagagcat ctcgcaggaa tccatgctaa cttccaggtc
     1801 ttctgggttg tcgatgttgc agcaggtcag ctgtctggac tgga
//
LOCUS       DAOSGP010000274         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_64439_length_1838_cov_15.401570, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000274 DAOSGP010000000
VERSION     DAOSGP010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64439_length_1838_cov_15.401570"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLV49_06825"
     CDS             complement(<1..935)
                     /locus_tag="PLV49_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98593.1"
                     /translation="MLLVMLLLPFTAAGHVPLIGGGNEDISSALHISNPAKSWAVYAV
                     LENETVHYFSFDVREGERIYMGLLKSTDPADEGFLPDLLLVGPGIGIQSSSPAQQPEG
                     NTSLEGAVLLENSAIPKNIILPESLEDLGALAADGREGIATYEPFGPSSFIGLAEINL
                     SAPQSGRYYAAVYENTRNGSNEGASLRAAEADAKGGHYCLVVGYREEFSFTDRMIAPI
                     QIASVYIWEGQSIIMILIPYLVALIAGGLIYWRGSRRSLFSLTGTLAGFLFLGTSSLV
                     ITQMIYSLSRAPYGPEVYITMAIILFHALLGVASIR"
     gene            1069..>1838
                     /locus_tag="PLV49_06830"
     CDS             1069..>1838
                     /locus_tag="PLV49_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOG98594.1"
                     /translation="MTRKNLIFLSLLFIAGWLCMGSCLAQNNTTAHGVLDAAGELGLT
                     EFSELAESTGLASTLDNQGVLLFDSGSFVIFAPSDDAFSAIDDMNMNILIENQTELER
                     VLSYHAVWNSGSFVNISDIDSARTLQGENISINSTDGLTVNGANVTESIQYDNGVIYV
                     IDKVLMPEKSSMAGAAKAAENLGAKDFASAIVAEGLEDRLNGQGLMGITTLGEGPFTI
                     FAPSDEAFDAAKSTVDSIKKQDMGMIDLLGYHMVEAKDL"
BASE COUNT          461 a          447 c          483 g          447 t
ORIGIN      
        1 cggatagatg ccactcccaa gagagcatgg aacaggatta tggccatggt gatatagacc
       61 tccggaccat aaggagcccg gctgaggctg tagatcattt gagttatgac cagagagctg
      121 gtgccgagaa acagaaagcc ggctaaagtc ccagtcaggc tgaacaagct gcgacgactt
      181 cctctccagt atatgagccc tccagcgatt aaagccacca gatatggaat gaggatcata
      241 atgatgctct gcccctccca gatgtaaacc gatgcgatct ggatcggagc gatcatcctg
      301 tcggtgaagc tgaactcctc gcgatatcct accactaaac aatagtggcc tcctttcgca
      361 tctgcttctg ctgctcttaa tgatgcccct tcattcgagc cgttcctggt gttctcatat
      421 actgcggcat aataccggcc cgattgaggt gcagagaggt tgatctctgc cagcccgatg
      481 aagctgctgg gcccaaaggg ctcataggtc gctattcctt cccttccatc ggctgcaaga
      541 gctcccaaat cctcaaggct ttcaggaagg attatgttct tgggaatagc agaattctca
      601 agaagaacgg cgccctcgag ggaggtgttg ccctcaggct gctgggcggg tgagctgctc
      661 tgaatcccga tacccggccc taccaagaga aggtctggca gaaagccctc gtctgccggg
      721 tctgtggatt tgagcagccc catatagatt ctctctcctt ctctgacatc aaagctgaaa
      781 taatgaacgg tttcgttctc taaaacggca taaaccgccc aggatttagc tggattagag
      841 atatgcaggg cagaggatat atcctcattt cctcctccta tgagaggcac atgtccggca
      901 gcggtaaatg gaagcaggag catgaccagc aacaaggcaa agatgactct ctcaggcgac
      961 acatcctcgc atagccgttc tatcagtatt gaatatttcg gcttatgcag aaggtttatc
     1021 ttttatccag ttgataaact atccagcgaa atgagtggag gttatgatat gacaaggaaa
     1081 aatttgatat tcctgagtct gttgttcatt gcgggatggc tttgcatggg ttcatgcctg
     1141 gcacaaaata atacaactgc tcatggagtc ctggatgctg ccggggaatt gggcctgact
     1201 gagttctcag aattggctga aagcacgggt ttagccagta ctctggacaa tcagggtgtc
     1261 ctcctcttcg attccggatc ttttgtgatc ttcgccccca gcgatgatgc tttttccgcc
     1321 atcgacgaca tgaacatgaa catcctcatt gagaatcaga cggagcttga gcgggttctc
     1381 tcttatcatg cggtctggaa cagcggttcc tttgtgaata tctccgatat tgatagcgca
     1441 aggactctgc agggtgagaa catcagtatc aacagcaccg acggtctgac ggtgaatggt
     1501 gccaatgtaa cagagagcat tcaatacgat aacggtgtca tctatgtaat cgacaaggtg
     1561 ctcatgcctg aaaagagctc gatggctggc gcggccaagg cagcagagaa tctgggtgca
     1621 aaggattttg cctctgccat tgtcgctgaa gggctggagg ataggctgaa tggccagggg
     1681 cttatgggaa tcaccaccct gggagaaggg ccgtttacca tcttcgctcc cagcgatgag
     1741 gcatttgatg ccgccaagtc cactgtcgat tcgatcaaaa agcaggatat gggaatgata
     1801 gacctgctcg gctatcatat ggttgaggca aaggatct
//
LOCUS       DAOSGP010000275         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_64865_length_1830_cov_19.722254, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000275 DAOSGP010000000
VERSION     DAOSGP010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64865_length_1830_cov_19.722254"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLV49_06835"
     CDS             complement(<1..788)
                     /locus_tag="PLV49_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG98595.1"
                     /translation="MVSARIASIHESTTLKISATAKKLTASGLDIIDMGVGEPDFDTP
                     KHIVEAGCNSIRMGETHYAPTGGIPELRQAIAEKLSRENLIHSAAEDVIVTPGAKMAV
                     FAAIQALLQEGEECLLIGPSWVSYEPSVAFAGGSVVWGEVDSDFMPQNIAEKISPKTR
                     MIIINSPSNPTGAVFERPVLEEIRDLAVDHDLFVISDEIYEKIIYDHEHISIGSLPGM
                     AERTVTINGFSKAYAMTGWRLGYLTGPKETMKWVIRLLSHSVSQA"
     gene            complement(792..1202)
                     /gene="ribH"
                     /locus_tag="PLV49_06840"
     CDS             complement(792..1202)
                     /gene="ribH"
                     /locus_tag="PLV49_06840"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOG98596.1"
                     /translation="MAKLGFVVSEFNRDITYQMELLGREHAQFLGADVAAVVYVPGVF
                     DMPIAVKKMLCREDIDAVVTIGCVIQGETAHDEVVVNHAARKLMDLSLECNKPVTLGI
                     TGPKMSRADAQKRVDYSKRAVEAAVKLLQRLGGD"
     gene            1316..>1830
                     /locus_tag="PLV49_06845"
     CDS             1316..>1830
                     /locus_tag="PLV49_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOG98597.1"
                     /translation="MSLIYLDGEYVPAEKAAVSVFDHGFLYGDGVFEGIRAYGGRVFR
                     LEDHVRRLFDSAQAIMLCIPLSQEEMALAIRDTLRKNNLRDAYVRPIVTRGYGDLGLD
                     PLKCRNPTVIIVAVEWGAMYGSLYEVGLTAISVSVRRNAPDALPPNIKSLNYLNNILA
                     KIEANVKGGNEA"
BASE COUNT          417 a          520 c          467 g          426 t
ORIGIN      
        1 gcctgggaaa cggaatgtga cagaagcctg atcacccact tcatcgtctc cttggggccg
       61 gtcagatagc ccagacgcca gccggtcatg gcataagcct tggagaatcc attaatggta
      121 accgttctct ccgccattcc cggcaaggag cctatgctga tgtgttcatg atcatagatt
      181 atcttctcat aaatctcatc ggagatcaca aagagatcgt gatctactgc caggtcgcgg
      241 atctcctcca gcacgggccg ctcgaagacc gctccggtgg gattagaggg ggagttgatt
      301 atgatcattc gggtcttggg gctgatcttc tccgcgatgt tctggggcat gaaatcagaa
      361 tcaacctcgc cccagaccac ggatccaccg gcaaaggcca cgctgggctc gtagctcacc
      421 caggagggac ctatcaatag gcactcctcg ccctcctgca agagcgcctg gatggcggca
      481 aagacggcca tcttagcccc tggagtcact atcacatcct cagctgcaga atgaatcaga
      541 ttctcccggg atagcttttc tgctatcgcc tgccgcagct cgggaatgcc gccggttggt
      601 gcataatgag tctcgcccat cctgatggag ttgcagcctg cctcgacgat atgctttggg
      661 gtgtcgaagt ccggctcgcc aactcccata tcgatgatat caaggccgct ggcagtgagc
      721 ttcttcgccg tggccgagat cttcagggtg gttgactcat gaatggaggc tatacgcgcg
      781 gataccattc tttaatcccc accgagcctc tgcaggagct tgaccgccgc ctccaccgcc
      841 ctcttggaat agtccaccct cttttgcgca tcagcccggg acatcttggg gccggtgatc
      901 cccagggtta ccggcttatt gcattcaaga gacagatcca tcagctttct ggctgcatga
      961 ttgaccacaa cttcatcatg ggcggtctct ccctgtatca cacagccgat tgtcacgacg
     1021 gcatcgatgt cctcgcgaca gagcatcttc ttcactgcta tcggcatatc aaaaactcca
     1081 ggaacataaa ccaccgctgc gacatctgct cccaggaact gggcatgctc cctgcccaaa
     1141 agctccatct gataggtgat gtccctattg aactccgaga cgacgaaacc aagtttagcc
     1201 attgacacca aaccccatga agagtaggca cctagctgcc ttccttccat aataaaccca
     1261 tcgattcaat caaaatgcat ttatccttgg taatgatgga gatacaggat tctctatgag
     1321 ccttatttat cttgatggtg aatatgttcc ggctgaaaaa gctgcggtct ctgttttcga
     1381 tcatggtttt ctctatggcg atggcgtctt cgagggcata agagcatatg gaggacgggt
     1441 cttccggctg gaggatcatg tccggaggct ctttgattct gctcaagcca tcatgctctg
     1501 tatccccctg agccaggagg agatggccct ggcgatcaga gacacgctgc gcaagaacaa
     1561 cctgcgcgat gcctatgtcc ggcctatagt caccagagga tatggagatc tgggcctgga
     1621 tcccctgaaa tgcaggaacc ccacggtaat catcgttgca gttgagtggg gggcgatgta
     1681 tggaagcctc tatgaggtcg gcctgaccgc catcagtgtc tctgttcgca gaaacgcccc
     1741 cgatgccctg cctccgaata tcaagagcct caattatctg aacaacatcc ttgccaagat
     1801 agaggccaat gtcaaagggg gaaatgaggc
//
LOCUS       DAOSGP010000276         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_65218_length_1824_cov_12.613341, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000276 DAOSGP010000000
VERSION     DAOSGP010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65218_length_1824_cov_12.613341"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..882)
                     /locus_tag="PLV49_06850"
     CDS             complement(202..882)
                     /locus_tag="PLV49_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HOG98598.1"
                     /translation="MITGCNWAGEEIVRDTIVYDKRVVIGSSGILIPTDVRDWLSHTH
                     SKVIARALEEMALPASREAGTFDMRAWRSWDYVTRSIDYVTDKSSFGLEDLWLFPEET
                     LMLGKGDCEDTSFLLASLLLASGISEQCVRVVLGRVASPAGSYGHAWVVYQCESGQWC
                     LLETTLESAPPSFTPADPFTLPGNQYQYQPQFCLNSSHLWSMTRMKTEFADYLKVRVK
                     PQQPLPSG"
     gene            1058..1516
                     /locus_tag="PLV49_06855"
     CDS             1058..1516
                     /locus_tag="PLV49_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOG98599.1"
                     /translation="MDIFMQAALDEARKGSREGGIPIGAVLVEEGRIIGRGRNRRVQQ
                     RDQMMHAEIDCLRNARLTGGYHNTVLYTTLMPCYMCAGAVVYFGIKKVVVGEAKSAPA
                     ARDFMEAHGIEVVDLDLAECREMMEEYIRENWKLWNEFLSELNPDLLIQQ"
     gene            complement(1586..>1824)
                     /locus_tag="PLV49_06860"
     CDS             complement(1586..>1824)
                     /locus_tag="PLV49_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98600.1"
                     /translation="YVHEGSLDGPILSDVQITGQDGDGNDFVGITNSSGVAIINGTPG
                     AWEFAFKKDGYDALYLEYNATQSEKAAAYLEKAD"
BASE COUNT          462 a          440 c          499 g          423 t
ORIGIN      
        1 cgaaaagggg atcgctctga ggatagatcc ccaaaggtgc accctgtgca acagccccct
       61 tctggaagtt gaggggcggg agggagagat gtggatatgt aatgtctgcc agaagctcta
      121 ctggcggggt ggtcactgga agagtatgga agaaacgcta aaagacctcc gcctgagaaa
      181 tcatgaaaga gatgaagatc atcaccctga tggcagtggt tgctggggtt tgactcgaac
      241 cttcagataa tccgcaaatt cggtcttcat ccgggtcatc gaccagagat gggaggagtt
      301 caggcagaac tgtggctggt attgatactg atttccaggc agggtgaatg gatcagcagg
      361 cgtgaagctg ggtggcgctg attccagcgt cgtctccaag aggcaccatt gtccagactc
      421 gcactggtag accacccagg catgcccgta ggaaccagct ggagaagcta cccggcccaa
      481 aaccacccgc acgcactgct ctgagatccc gctggccagg agaagagaag ctaagagaaa
      541 agaggtatcc tcgcagtccc cttttccgag catcagcgtc tcctcgggaa agagccagag
      601 gtcttccagt ccaaagctgc tcttgtcggt gacgtagtct attgacctgg tcacgtagtc
      661 ccagctccgc caggctctca tgtcgaatgt ccctgcctcg cgggaggcag gaagggccat
      721 ctcctccagg gctctggcta ttaccttgct atgggtgtga gatagccagt cccggacgtc
      781 agtgggaatc agtatgcccg agctgccgat taccactctc ttatcataaa cgatcgtgtc
      841 cctgacgatc tcctcccctg cccagttaca tcctgtgatc atgatacccc caatcccatc
      901 tatgttaata attttagttc actatattta aaattatcga aattgactgt ttttggatat
      961 cgatgcttgc taagataaat tcattacata tatttaataa aaattctatg agatgagcaa
     1021 agcttgatca atcttgccat ccaatttgat attcaacatg gatatcttca tgcaggcggc
     1081 gctggatgag gcccgcaagg gctccagaga aggtgggatt cccataggag cggtcctggt
     1141 tgaggaggga cggatcatag gaagaggaag gaacagaaga gtgcagcaaa gagatcagat
     1201 gatgcatgcg gagatcgact gcctgcgcaa tgcccggctg accggcggct atcacaatac
     1261 agtgctctat acaaccctca tgccctgcta catgtgcgcc ggggcagtgg tctacttcgg
     1321 gatcaagaag gtagtggtcg gcgaggcaaa gagcgctccc gctgccaggg acttcatgga
     1381 ggctcatggg atagaggtag tggacctgga cctggctgag tgcagggaga tgatggagga
     1441 atacataagg gagaactgga agctatggaa tgagttcttg tcagagctaa acccggatct
     1501 tctgatccag cagtgaaaat gaaataagaa agaattgagg aagggaggct ttgtgccgcc
     1561 cattgcattt gttttgccag attattcagt cagccttctc cagatatgcc gctgcttttt
     1621 cgctttgcgt ggcattatat tccaggtaaa gggcgtcata accatctttt ttgaaggcaa
     1681 actcccaggc gcctggagtc ccgttgatga tggcgacccc ggaggagttc gttatcccga
     1741 caaaatcatt tccatctcca tcctgccctg tgatctgaac atcagagaga attgggccat
     1801 ccagacttcc ttcatggaca tata
//
LOCUS       DAOSGP010000277         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_65334_length_1822_cov_17.840407, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000277 DAOSGP010000000
VERSION     DAOSGP010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65334_length_1822_cov_17.840407"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PLV49_06865"
     CDS             complement(<1..1430)
                     /locus_tag="PLV49_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOG98601.1"
                     /translation="MNTRGKRPEAQNRTILQKVARGIMLMRGLQTDFSPECLSELARI
                     NGPAKADGAPHIRDLRGLLWASIDNDDSEDLDQLTVAEPLDDGKARIRVAVADVDSLV
                     HKDSAIDLDAQANTTSVYTAAQIFPMLPLKLSTNLTSLNLDQDRLAVVVDMTVAPDGS
                     LVDSDVYRAVVHNHAKLAYNSVAAWLDGAEPMPEAVSAVDGLAENLLLQDKVAQNMKS
                     LRHAQGALSLETIEAKPVFKGERLCDLLVEETNRAKQIIEDFMVAANGVTVRYLTERN
                     MPTIRRVVRVPKRWDRIVQIAAQHAFKLPPNPSSRKLEIFLNMMKKKDPLRFPDLSLA
                     VIKLLGNGEYVAELPGTEPIGHFGLAVKDYAHSTAPNRRYPDLITQRLLKAAIEKKPL
                     PYTIRELDRLADHCTRKEDIVAKVERQVSKSAAALLLEPRIGERFSAMVTGSSEKGTW
                     VRLLDIPVEGMLKGKRKGIDVGDEVTV"
     gene            complement(1449..>1822)
                     /locus_tag="PLV49_06870"
     CDS             complement(1449..>1822)
                     /locus_tag="PLV49_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98602.1"
                     /translation="FVDQIFAQAYRNLSESTGQDLRDPFSDPCASQYQLLDELRSYGR
                     RDLPEPFLRFIRAEFKKAFIPTMRLLTDLCHSENKYSWEEVKLQLVEIMDHLGVDVTW
                     EECEEKLETYLKKIGGTIYIN"
BASE COUNT          341 a          521 c          530 g          430 t
ORIGIN      
        1 acagtgacct catctcccac atcgattccc tttctttttc ctttgagcat cccttccact
       61 ggaatgtcaa gcagccgcac ccaggtgccc ttctccgatg agccggtgac catggcgcta
      121 aacctctccc cgatccgggg ctcgagcaga agtgcagcag ctgacttgct cacctgccgc
      181 tccaccttgg ccacgatatc ctctttccgg gtgcaatggt cggccagccg gtccagctca
      241 cgtattgtgt agggcaaggg ctttttctca atggccgcct tgagcagcct ctgggtgata
      301 agatcgggat agcgccggtt gggggcggtg gagtgggcgt aatccttgac cgccaggccg
      361 aagtggccta tgggctctgt gccgggcagc tccgccacat actcgccatt tcccagcagc
      421 ttgattacgg ctaaagatag atcggggaag cggaggggat ctttcttttt catcatgttc
      481 aggaatatct ccagctttct ggagctggga ttaggaggaa gcttgaatgc atgctgagcg
      541 gcgatctgca cgatccggtc ccacctcttg ggaacccgca ccactctgcg aatggtgggc
      601 atatttcgct cagtcagata gcggacggtt actccattgg cggcgaccat gaagtcctcg
      661 atgatctgtt tggctcggtt agtctcctcc actaaaaggt cgcagagcct ctctcccttg
      721 aagaccggtt ttgcctcaat ggtctccagg cttaaagcgc cctgggcgtg gcgtaggctc
      781 ttcatattct gggccacttt atcctggagg agcaggttct ccgccaggcc gtctacggcg
      841 cttaccgcct cgggcatggg ctcagcccca tccagccagg cggcaacgct gttataagcc
      901 agcttggcgt ggttatgcac caccgcccgg tagacgtctg agtcgaccag cgagccgtcg
      961 ggggcaacgg tcatatccac caccaccgcc aggcggtcct ggtctaggtt aagagaggtg
     1021 aggttggtgg agagcttcag aggcagcatg gggaagatct gggcggcggt gtagacggag
     1081 gtggtgttgg cctgagcatc aaggtcgatg gcagagtcct tgtggaccag gctgtccacg
     1141 tctgccactg ctaccctgat cctggccttt ccgtcgtcca ggggctcggc cacggtaagc
     1201 tgatccaggt cctcggagtc atcgttatct atggaggccc agagcagccc tctcaggtcg
     1261 cggatatggg gggcgccatc agccttggct ggaccgttga tgcgggcgag ttcagagagg
     1321 cactccggcg agaaatcggt ttgaaggcct cgcatgagca ttattcctct ggcaactttc
     1381 tgcaggatgg tccggttctg agcttctggt cttttgcccc tggtattcat gaattattcc
     1441 gaaagaactc aattgatata aatggttcca cctatctttt tcagatacgt ctccagcttc
     1501 tcctcgcact cttcccaggt cacatctact cccaagtggt ccatgatctc cacgagctgc
     1561 agcttgacct cctcccagga gtacttgttc tccgaatggc acaggtcggt caatagcctc
     1621 atggtgggga tgaaggcctt cttgaactcc gcccggatga atctcaggaa aggctctggc
     1681 aggtccctcc ggccatagga cctcagctca tccaggagct ggtactggct ggcgcaggga
     1741 tctgagaagg ggtctcgcag atcctgaccg gttgattcag agaggttccg gtaggcctgg
     1801 gcgaagatct ggtccacaaa tg
//
LOCUS       DAOSGP010000278         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_65437_length_1820_cov_13.223229, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000278 DAOSGP010000000
VERSION     DAOSGP010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65437_length_1820_cov_13.223229"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLV49_06875"
     CDS             <1..186
                     /locus_tag="PLV49_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719886.1"
                     /note="catalyzes the reduction of
                     methylenetetrahydromethanopterin to
                     methyltetrahydromethanopterin with the oxidation of
                     coenzyme F420; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydromethanopterin reductase"
                     /protein_id="HOG98603.1"
                     /translation="AMDGVTTKMTEAFSISGAPADCRARIDELLSTGVTQIVVGSPIG
                     PNKESAIKLIGKQVIGK"
     gene            209..769
                     /locus_tag="PLV49_06880"
     CDS             209..769
                     /locus_tag="PLV49_06880"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HOG98604.1"
                     /translation="MRGLYIGRFQPYHLGHQAVLEEIAREVDEIVIVIGSAQESHGPE
                     NPFTSGERMEMIYAALGKGELRKRCIVTTLQDINRNSLWVSHLQSMVPCFDLVYSNNP
                     LVIRLFSEAGVEVKKPPLYQRDVYSGSAIRVLMREGGDWERLVPPAVAGFIQEINGVE
                     RLCQVSKSDSASSADSAMHSSKETDF"
     gene            complement(766..1788)
                     /locus_tag="PLV49_06885"
     CDS             complement(766..1788)
                     /locus_tag="PLV49_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOG98605.1"
                     /translation="MILGLDIGGANTKAASAEGKYAKSVYLPLWKNSPLEKVLKRINA
                     EQKPEAVAVVITGELADCFSSKIEGLERIMTVACRAFSCPVYFWGHDGFARNDIINPT
                     SMTSNVDMINTTDILHIAAANWSASAALLAREIGDCLFVDMGSTTTDLIPIRARPLAA
                     KTDFLRLARGELVYMGLLRTGLACILPVARIRGHHVPLAPELFAIIADARLVLGHIDL
                     DRYTCDTADGKDKSRSSSLRRLTRSVCADLEEIGESGALAIAEQACTEQKRTLTEAME
                     RQAERHGLERVAAAGIGEWLIAEAANTLGIECILLSELYGSRISDVFPAYAAARLLEM
                     DPVFIH"
BASE COUNT          420 a          512 c          506 g          382 t
ORIGIN      
        1 gcgatggatg gcgtgaccac taaaatgaca gaggccttct ccatatccgg agcgcctgca
       61 gactgcaggg caaggataga cgagctttta tcgaccggag tcacccagat tgtggtcggc
      121 tcgcccatag gtcccaataa agagagcgcc ataaagctca tcggaaagca ggtcatcgga
      181 aaataggcca gaaaggaagg tcaaggctat gcgcgggctt tacattgggc gatttcagcc
      241 ctatcacctc ggccatcagg cagttctaga ggagatcgca cgagaggttg atgagatagt
      301 gatagtgatc ggaagcgccc aggagagcca tggcccggag aaccccttca ccagcgggga
      361 gaggatggag atgatctatg ccgccctggg gaaaggagag ctgcggaaga ggtgtatcgt
      421 caccaccctg caggatatca atcgcaactc attgtgggtc tcacatctgc agtccatggt
      481 gccctgcttt gacctggtct actccaacaa ccccctggtc atccggctct tctccgaggc
      541 gggggttgag gtgaaaaagc cgcccctgta tcagagggat gtgtactctg gatcggccat
      601 ccgcgttctc atgcgggagg gaggagactg ggagaggctg gtgcctccgg cagtagcggg
      661 tttcatccag gagatcaacg gggtagagcg gctctgtcag gtatcaaaga gcgacagcgc
      721 ttcttccgcg gattcggcaa tgcatagctc caaggagaca gatttttaat gtataaagac
      781 cggatccatc tccagcagcc tggctgcggc ataggcaggg aagacatccg agatcctgct
      841 gccatacaat tcagagagca gaatgcactc aatgcccagt gtattggccg cctcggcaat
      901 cagccactct ccaattcccg ctgcagccac cctttccagt ccatgccttt ctgcctgcct
      961 ctccatggcc tcagtgagag ttctcttctg ctctgtgcag gcctgctccg ctatggccaa
     1021 agctccgctt tcccctatct cctccagatc ggcgcagacc gagcgcgtca accggcggag
     1081 ggatgaagat cggctcttgt cttttccgtc tgcagtatcg caggtatatc tatccagatc
     1141 tatatggccc aaaacaagac gagcatcggc gatgatggcg aaaagctctg gggcaagcgg
     1201 cacatgatgg cctctgatcc tggccacggg cagaatgcag gcaagaccgg tgcgcaggag
     1261 gcccatatag accagctctc ctctggccag gcggaggaaa tcggtcttcg ctgccagcgg
     1321 cctagcccta atgggaatca ggtctgtggt ggtgctgccc atgtccacaa agaggcaatc
     1381 acctatctcg cgagcaagaa gcgcagccga ggcggaccag ttggctgcgg cgatatgcag
     1441 aatatcggtc gtgttgatca tgtcaacatt gctggtcatg ctagtcggat ttatgatgtc
     1501 gtttcgggca aatccatcat ggccccagaa ataaaccgga caggagaagg cacggcacgc
     1561 tacagtcatt attctctcca atccctctat cttggaggag aagcagtctg ccagctcgcc
     1621 agtgatgacc accgccacag cctccggctt ctgctcggca ttgatcctct ttaagacctt
     1681 ctccaggggc gagttcttcc aaaggggaag atagacactt ttggcatatt tgccttccgc
     1741 actcgccgct ttggtgttcg cccccccaat atccagaccc aggatcatct acgctcaaac
     1801 ctcattcgaa ccataaaagc
//
LOCUS       DAOSGP010000279         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_65647_length_1816_cov_19.890403, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000279 DAOSGP010000000
VERSION     DAOSGP010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65647_length_1816_cov_19.890403"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLV49_06890"
     CDS             complement(<1..352)
                     /locus_tag="PLV49_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98606.1"
                     /translation="MFSEKDLVARSIEDMAQEVEELQALAREEREKSEALSQKEIELR
                     RKSVETRSVNAELAESFWQEAESLRDESRELMRLFMEHKLRAAEVQHRIDIHDQIESL
                     DDYDEVWRQAASARR"
     gene            complement(354..932)
                     /gene="pdxT"
                     /locus_tag="PLV49_06895"
     CDS             complement(354..932)
                     /gene="pdxT"
                     /locus_tag="PLV49_06895"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOG98607.1"
                     /translation="MPKIGVLALQGDVSEHVDALQRIGEGIDVLEVRKPGQIASCSAL
                     VLPGGESTTIVQQLNSSGLAGELEAAATKGLPILATCAGLVLISREIEGDNHVHPLGL
                     MDIKISRNAFGPQRESFEADLDVVGMDRPYRAVFIRAPAVSEVGRDVQVLAKVGGLPV
                     AVREKNLLGLAFHPELTDDMRFHQLFLKMLEA"
     gene            complement(933..>1816)
                     /gene="pdxS"
                     /locus_tag="PLV49_06900"
     CDS             complement(933..>1816)
                     /gene="pdxS"
                     /locus_tag="PLV49_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOG98608.1"
                     /translation="NLRFGTELLKRGFAKMQKGGVIMDVTNPEQAIIAEEAGAVAVMA
                     LHAVPADIRKMGGVARMADPLVIESIIEAVTIPVMAKARIGHFVEAQILEALGVDMVD
                     ESEVLTPADEFHIEKTKFTIPFVCGARNLGEACRRIKEGAAMIRTKGEAGTGDVSQAV
                     LHMRMILGQIRSLKGMDDLQLMKVAREIEADLDLVRECAQMQRLPVVNFAAGGVATPA
                     DAALMMQLGADGVFVGSGIFKSESPEAMARAIVEAVHHYDDPAHLAKVSKGLGAAMKG
                     LDAASIPEAEALQMRGW"
BASE COUNT          365 a          626 c          418 g          407 t
ORIGIN      
        1 cccgtctggc actggctgcc tgtctccaga cctcatcata atcatccaga gactcgatct
       61 ggtcatgtat atcgatgcga tgctgaacct cagccgcgcg cagcttatgc tccataaaaa
      121 gtcgcatcaa ctcacggctc tcatctcgga ggctctctgc ctcctgccag aagctctccg
      181 ccagttcggc attcactgat ctcgtctcca cggacttccg gcgcagctcg atctccttct
      241 gactgagagc ttcgctcttc tccctctctt ccctggccag agcctgcagc tcttctacct
      301 cttgagccat atcctctatg cttctggcca ccagatcctt ctctgagaac atatcaagcc
      361 tccagcatct tcaaaaacag ctggtgaaat ctcatatcat ccgtcagctc agggtggaag
      421 gcaaggccga gcaggttctt ttcccggaca gcgaccggca aaccacccac cttcgccagg
      481 acctgcacat ctcgtcccac ctcggatacc gcaggagcgc ggatgaagac cgccctataa
      541 ggacggtcca tccccactac atccaggtcc gcctcaaagg actccctctg agggccgaaa
      601 gcattccggc tgatcttgat atccatcagg cccaggggat gaacatgatt gtcgccctct
      661 atctctctgg agatcaacac cagaccggca catgtggcaa gaatgggcaa gcccttggtc
      721 gcagctgcct ccagctcccc ggccaggccg gagctattca attgctggac gatggttgtg
      781 ctctctccgc caggcaaaac cagggcggag caggacgcga tctggccggg ctttctgacc
      841 tccagcacgt ctatgccttc cccaatcctc tgcagagcat cgacgtgctc cgagacatcg
      901 ccctgcaggg ccagcactcc tattttaggc acctaccagc ctctcatctg cagggcttcg
      961 gcttcgggaa tgctagccgc atccagcccc ttcatggccg cccccagacc ctttgagacc
     1021 ttggccagat gagcaggatc atcatagtga tgcaccgcct ccactatcgc tctggccatc
     1081 gcctccgggc tctctgactt gaatattccc gagcccacga agaccccgtc agcgcccagc
     1141 tgcatcatga gagcagcatc tgcaggcgtt gccaccccac cggcagcgaa gttgaccacc
     1201 ggtagacgct gcatctgggc gcactccctg accagatcca gatcggcctc gatctccctg
     1261 gccactttca tcagctgaag atcgtccatt cccttcaggc tcctgatctg gcccaggatc
     1321 atgcgcatat gcaagaccgc ctggctcaca tctccggttc ctgcttcacc cttggttctg
     1381 atcatggctg caccctcctt tatccgccgg caggcctctc ccaggttccg ggctccgcag
     1441 acgaaaggaa tggtgaactt cgtcttctca atatgaaatt cgtcagcagg cgtcaagacc
     1501 tcggactcat ccaccatatc cacgcccagt gcttccagta tctgggcctc aacgaagtgg
     1561 ccgatcctgg cctttgccat gacgggaatg gtcaccgcct ctatgatcga ctcaatgacc
     1621 aggggatctg ccatcctggc cactccgccc atcttcctga tatcagcggg aacggcatgc
     1681 aaggccatta ccgcaacagc acctgcttct tccgctatta tggcctgttc agggtttgtg
     1741 acatccatga tcactccccc tttctgcatc tttgcgaaac cgcgtttgag cagttccgta
     1801 ccaaatcgaa gatttt
//
LOCUS       DAOSGP010000280         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_66077_length_1808_cov_18.762122, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000280 DAOSGP010000000
VERSION     DAOSGP010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66077_length_1808_cov_18.762122"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..876)
                     /locus_tag="PLV49_06905"
     CDS             complement(154..876)
                     /locus_tag="PLV49_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG98609.1"
                     /translation="MTEDDGSSGESLYLRGDYEEAVAAWERSENGTEGFEHHYWKGVA
                     LRKLGRNEEAVCALEKALSLNPESADAWRNHAVALNQLDRGADALKSCQKALAIDPDN
                     PRTWIVRGFALHILGRFEEAVESYARAIELNPLGPDGRRAWNNRGAALDNLHRHEEAI
                     ESYEEAIMIDPFDIYPWNNKGVSLSALGRQEAAVVCFWKAIEIDPEYAVAWKNLAVAY
                     RSLHRDQEAEEAARIARDLGLS"
     gene            1088..1357
                     /locus_tag="PLV49_06910"
     CDS             1088..1357
                     /locus_tag="PLV49_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HOG98610.1"
                     /translation="MNFLDLDSDGLRRVVLESMVQSQEFTVLSLHEEVSKEVNVSRKV
                     VASMTGYIGSRLGILHMNKKSYRTPRTYALKDEYADIARAVLASL"
     gene            1354..1755
                     /locus_tag="PLV49_06915"
     CDS             1354..1755
                     /locus_tag="PLV49_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98611.1"
                     /translation="MTNVLKAPRTATTLKVRSKSEYAISRSRDPYHELMLRLFQEEAT
                     AERGQKFLVIVEERQKRGDPLKTSEWRELLVELKVSRSAFYAMRNKLLGAGMITNKGG
                     EYRLSGIFSRDLMDMARWWWTAVLGNNLENL"
BASE COUNT          452 a          481 c          465 g          410 t
ORIGIN      
        1 accagtggtc caaccaccgc ctggcaaaga agatccgggg atccttccgg gtattgacag
       61 taaccaggga aaacagggat tctctggccg atgagattgc caaggccctg aaatcggggt
      121 gacgctgcca agatcaaaac cccgccgtct ggatcagctc aaccccagat cgcgcgcgat
      181 gcgagcagcc tcttctgcct cttgatcgcg atgcaatgat cggtaggcta ctgccaaatt
      241 cttccaggca actgcatact caggatcgat ctcgatcgcc ttccaaaagc ataccaccgc
      301 cgcctcctgt cggccaaggg ctgataagga aacccctttg ttattccagg gatagatgtc
      361 gaaaggatcg atcattatcg cctcctcata actctctatg gcctcctcat gccggtgcag
      421 gttgtctaag gcagctcctc gattgttcca ggccctcctc ccgtccggtc caaggggatt
      481 cagctcgatg gcccgggcat agctctccac cgcctcctcg aaccggccga ggatatgaag
      541 ggcaaagcct ctgacaatcc aggtccgtgg attatcagga tcgatggcca gagccttttg
      601 gcaggattta agggcatcag cgccccgatc aagctggttt agtgccactg catggtttct
      661 ccaggcatcc gcgctctcag gattcaagga gagggccttc tccagggcac acactgcctc
      721 ttcattacgg ccaagcttgc gcagagccac gcctttccag taatggtgct caaatccttc
      781 cgtcccattc tctgatcgct cccaggcagc gaccgcttcc tcgtaatctc cccgcagata
      841 gagactctct ccgcttgatc catcatcctc tgtcatagca ccgccgaacc cgtcgcatta
      901 tatccatatc cccatttatc cttaaaccaa tggcggtgag tctgaaagca taaggatagc
      961 atatattctg gattcatatt agtaaggtat atatataata aatattacta aagtattagc
     1021 aatgaaaggg agtggtacgg agggaggttc cacgaaatcg cagcgatctg agatacaggg
     1081 cagaatgatg aatttcctcg atctggatag cgatggtctg aggagggtag ttctcgagtc
     1141 catggtccag tcacaggagt ttaccgtttt atctcttcat gaagaggtga gcaaggaggt
     1201 caacgtatcc aggaaggttg tggcctccat gaccggctac ataggctccc gcctggggat
     1261 attgcacatg aacaagaaat catatcgtac ccccagaacc tatgccctga aagatgaata
     1321 tgcagatata gccagagcag tactggctag cttatgacca atgtgctcaa ggccccaagg
     1381 actgccacta cattaaaggt ccgctccaaa tcggagtacg caatcagccg atctcgagat
     1441 ccataccatg agctgatgct acgccttttc caggaggagg caaccgcaga gagggggcag
     1501 aaattcctgg tcattgttga ggaaaggcag aagagaggag atccattgaa gaccagtgaa
     1561 tggagggagc ttttggtcga actaaaagtg agccggtctg ccttttatgc catgagaaac
     1621 aagctgctgg gtgcgggaat gatcaccaat aagggcggag agtacagact ctcgggcata
     1681 ttcagccgag atctgatgga tatggcccgg tggtggtgga cggcggttct gggcaataat
     1741 ctggagaacc tgtaaaggct catacgcagg cctttaaatc cgtgatctgc ctttctatgg
     1801 cctctacc
//
LOCUS       DAOSGP010000281         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_66430_length_1801_cov_18.774341, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000281 DAOSGP010000000
VERSION     DAOSGP010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66430_length_1801_cov_18.774341"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="modA"
                     /locus_tag="PLV49_06920"
     CDS             <1..754
                     /gene="modA"
                     /locus_tag="PLV49_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG98612.1"
                     /translation="VFTAASLTGAFGEIGEMYENETGIHVAFNFDGSQALRTQLENGA
                     YADLFASANMKQMNAVRESRLMNNSSVAIFTRNKLSLIVPKDNPANIRNLTDLARPGV
                     KIVMGTKDVPVGDYALQIIAKLGNDSAYGPDYETEVLANVISQETNVNYVVTKVALGE
                     ADAGFAYVSDVTQDMISKIDKIVIPDEYNIIAEYPLGMLMESKYPAESQRFMDLVMSD
                     EGRAVLEKYGFDPVESEPEVREETASATEAAA"
     gene            830..1723
                     /locus_tag="PLV49_06925"
     CDS             830..1723
                     /locus_tag="PLV49_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98613.1"
                     /translation="MASNQIDINRLGKRDLAELAGRGQESISILSRVREVALKAGLAL
                     LLIIAMTFIALPVVALFLRSPLDTTLRSLHDPVVMDALRLSLMTSTVTTITVVLMGTP
                     IAYVSARFRYFGKELADSLIDLPVIMPPAVAGIALLMAFGRMGILGRHLDGLGISIAF
                     TTLAVIMAQVFVSSPFFIRQARTSFEDVDIALENAARTLGGSRVYTFFHVILPIAVNG
                     LVSGAIMAFARSLGEFGATIMFAGNFQGRTQTMPLAIYTAMQGDLDVSLCIAIILVAI
                     SFVVIFLVKTLTRRVYKNAGN"
BASE COUNT          434 a          451 c          486 g          430 t
ORIGIN      
        1 agtctttaca gcagcttcgc tgacaggagc ttttggcgaa ataggagaga tgtacgagaa
       61 cgaaaccggc atccatgtgg cattcaactt cgacggctca caggcactgc gcacccagct
      121 cgaaaacggt gcttatgccg atctcttcgc ttcagccaat atgaagcaga tgaatgccgt
      181 aagagagagc aggctaatga acaacagcag cgtcgccatc ttcacaagaa ataagctctc
      241 gctcattgtc cccaaggata accccgcaaa catccggaat ctgaccgacc tcgcccggcc
      301 gggtgtcaag atcgtcatgg gaacaaagga tgtgccggta ggcgactatg ctcttcagat
      361 catagccaag cttggcaacg actctgcata tggtcctgac tatgagacag aggtgctggc
      421 caatgtcatc tctcaagaga ccaatgtcaa ctatgtggtg accaaagtgg ccctgggaga
      481 ggcggatgcc ggatttgcat atgtctccga tgtcacccag gatatgataa gcaagataga
      541 taagatagtt atcccggacg aatacaatat catagccgaa taccccctgg gaatgcttat
      601 ggaatcaaag tatcctgccg agagccagag gttcatggat ctggtgatgt cggatgaggg
      661 aagggcggtc ttggaaaagt acggctttga tcccgtcgaa agcgagcctg aggtgcgtga
      721 ggagactgct tcggcaacag aagctgcagc ttaaccggat gatctctttt tttttgagcc
      781 agggacagaa ataaaaccaa gagaatttaa tgaagagatc gagtgaccaa tggcttccaa
      841 tcagatagat atcaatcgtc tcggcaaaag ggatcttgct gagctggcag gtcgggggca
      901 agagagcatc agcatccttt ccagggtgag ggaagtggct ctaaaagcgg gtttggccct
      961 cttgctgata attgccatga ccttcatagc tctgcctgta gtcgcgctct tcctcaggag
     1021 ccctctggac actactctcc gatctcttca tgatccagtg gtaatggatg cactcagact
     1081 cagtcttatg acctccacgg tgaccaccat cactgtggta ttaatgggaa cgcctatcgc
     1141 ctacgtcagc gctcgatttc gatatttcgg caaggagctg gccgactccc tgattgatct
     1201 gcctgttatc atgcctcctg cagtggcagg aattgccctg ctcatggcct ttggccggat
     1261 gggaatattg ggccggcacc tggacggttt gggaattagc atcgccttca ccactctggc
     1321 ggtgatcatg gcccaggtat tcgtctccag cccctttttc atccgccagg cgaggacgag
     1381 cttcgaggat gtggatattg ctctcgagaa tgcggctcgg acccttgggg gttcacgggt
     1441 ctacaccttc tttcatgtca tcctgcctat agccgtcaac ggcctcgtct ccggggccat
     1501 aatggccttc gcccgctctc tgggagagtt tggagctact atcatgtttg ccggcaactt
     1561 tcagggcagg acgcagacca tgcctctggc catctacaca gccatgcaag gcgatctgga
     1621 cgtctccctg tgtattgcca taatattggt ggccatttca tttgtggtca tattcctggt
     1681 aaagactctg acccggcggg tgtataagaa tgctggaaat tgacatcaaa aagaggcttc
     1741 gggatttcga tctcgaggtt aagatgatga tcaaagaagg cgagatcctg atgctggtgg
     1801 g
//
LOCUS       DAOSGP010000282         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_66482_length_1800_cov_17.339255, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000282 DAOSGP010000000
VERSION     DAOSGP010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66482_length_1800_cov_17.339255"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /gene="carB"
                     /locus_tag="PLV49_06930"
     CDS             complement(<1..1742)
                     /gene="carB"
                     /locus_tag="PLV49_06930"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOG98614.1"
                     /translation="MPKREDINKIMIIGSGPIVIGQACEFDYSGTQACKALKALGYKI
                     VLVNSNPATIMTDPGMADATYIEPLNVERLAQIIEKERPDALLPNLGGQNGLNLSLEL
                     ARAGILDKFNVMVIGVKLDAIERGEDRIIFKETMNSLGIEVPKSTAIYTVEDAEKVAL
                     DLGYPVVVRPAYTMGGTGGGLVYNAEELRTVVSRGIAASLIGQVLIEESVAGWEELEL
                     EVVRDSKNHMITVCFIENVDAMGVHTGDSFCTAPMLTISQELQDKLQDYSYRIVEAIK
                     VIGGTNIQFAHDPKTGRVVVIEINPRTSRSSALASKATGFPIALISAKLASGLTMDEI
                     PYWKEGTLEKYTPSGDYVVVKFPRWAFEKFRGSKDVLGTQMKAVGEAMSIGKSYKEAF
                     QKAIRSLENSRYGLGFARDYNSRSPEELMMLLKETSSERQFILYEAIRKGADIQKLYQ
                     LTHIKPWFLQQMKELVQLEEEILAYKGKPLVDLPDELLRRAKRDGFADRYLSKLLAIP
                     EAEIRARRKSLGIVQGWEPVPVSGVENAFYYYSTYNAPDRTPSSSRRKVMVLGGGPNR
                     IGQGIEFDYCCVHAA"
BASE COUNT          353 a          531 c          482 g          434 t
ORIGIN      
        1 gcggcatgaa cgcagcagta gtcgaactca atgccctggc cgatgcggtt gggccctcca
       61 cccaagacca tgaccttgcg cctgctgctg ctcggggtcc tatcgggagc attgtatgta
      121 gagtaatagt agaaggcgtt ctccaccccg cttacaggta ccggctccca gccctgaaca
      181 attcccaggg acttgcgccg cgccctgatc tcagcctctg ggatggccaa gagcttcgag
      241 aggtatcggt ctgcaaagcc atctcttttg gcccgacgaa gaagctcatc tgggagatcg
      301 accagtggtt tacccttgta ggccaatatc tcttcttcca gctggaccaa ttccttcatc
      361 tgctggagga accagggctt gatatgggtc aactgataga gcttttggat gtcggcaccc
      421 tttcggatcg cttcatagag aatgaactgc cgctcgcttg aagtctcctt aagaagcatc
      481 ataagctctt ccggagatct gctgttgtag tccctggcaa agcccaggcc gtacctgctg
      541 ttctccaaag agcgtatggc cttctggaag gcttccttgt agctcttgcc gatgctcatc
      601 gcctctccca ctgccttcat ctgggtgccg agtacgtcct ttgagccccg gaacttctcg
      661 aaggcccagc gggggaactt gaccacaaca taatctcccg agggcgtgta tttttccagt
      721 gtcccctcct tccagtaggg tatctcatcc atggtcagac cagaggcaag ctttgccgag
      781 atcagggcga tgggaaagcc ggtagccttg gaggcgaggg cggatgagcg ggatgtcctt
      841 ggattgatct ctataaccac cacccttccg gttttcggat cgtgggcgaa ctggatattg
      901 gtcccgccga tgaccttgat ggcctcaacg atccggtagg agtagtcctg cagcttatcc
      961 tgaagctcct gggatatggt tagcatgggg gcggtacaga aggaatcgcc ggtatgaact
     1021 cccatggcgt ccacgttctc tatgaagcag acggtgatca tatgattctt ggaatccctg
     1081 accacctcca gctccagctc ctcccatcct gcgaccgact cctcgatgag cacctggccg
     1141 atcaggctgg cggcaatacc ccggctgacc acggtcctca gctcttcggc gttatagacc
     1201 agaccgcctc ctgttcctcc catggtgtag gccggccgga cgaccaccgg gtagccgaga
     1261 tccagggcca ccttttctgc gtcctccacc gtatagatcg cggtgctctt ggggacctcg
     1321 atccccaggg agttcatggt ctccttgaag atgatcctgt cctcgccccg ctcaatggca
     1381 tccagcttca cccctatgac cataacattg aacttgtcca ggatgcctgc gcgggcgagc
     1441 tcaaggctca ggttgaggcc gttctgcccg cccaggttcg gtagcagggc atcaggccgc
     1501 tccttttcga tgatctgggc cagcctctcg acattgagcg gctcgatata ggtggcatcg
     1561 gccatgcccg gatcggtcat aatggtggcc ggattggagt tgacaaggac tattttgtat
     1621 cccagggcct tgagcgcttt gcaggcctgg gtgccagagt aatcgaattc gcaggcctgc
     1681 ccgattacga tagggccaga cccgatgatc atgatcttgt ttatatcttc acgcttcggc
     1741 atctttcccg acctcttact tgaacgataa tagagcaaac gaattcctat tttatccctt
//
LOCUS       DAOSGP010000283         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_66648_length_1797_cov_17.858209, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000283 DAOSGP010000000
VERSION     DAOSGP010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66648_length_1797_cov_17.858209"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLV49_06935"
     CDS             <1..284
                     /locus_tag="PLV49_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HOG98615.1"
                     /translation="RIYDHLRAEMKGEWKEKDTEEQFIYKTRKKAFGPFKPELWQLPT
                     DVRDEICKELEGKFAFLFEKLMVMGTRPVLDQYIKPVDVPLEMPKMLKG"
     gene            379..1494
                     /gene="fbp"
                     /locus_tag="PLV49_06940"
     CDS             379..1494
                     /gene="fbp"
                     /locus_tag="PLV49_06940"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HOG98616.1"
                     /translation="MTKMTISLIKADVGGYPGHCSVHPELIKTAEEKLEEAKKSGCLV
                     DYRVMACGDDLELLMSHRTGCDCGDIHGLAWETFELATEKAKELKLYGYGQDLLADAF
                     SGNIKGMGPGVAEMEITERTAEPIIAFMMDKTEPGAFNLPIFKMFADPFNTAGLIIDP
                     SFHHGFSFEVWDILEHKKVLMNCPEEMYDMLALIGAKSRYVIKRVFAKPGSKIPQSEP
                     VAVISTEKLYQTAGKYVGKDDPVALVRSQSGLPALGEVLEPFALGHLVSGWMRGSHNG
                     PLMPCSFDTAHPTRFDGPPRVIAAGFQMAEGKFVGPVDLFKDVAFDRVRQRCLEITDY
                     MRAHGPFEPHRLPMEEMEYTTLPHVMNCLRDRFVDTE"
     gene            1572..>1797
                     /locus_tag="PLV49_06945"
     CDS             1572..>1797
                     /locus_tag="PLV49_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98617.1"
                     /translation="MNLPDDDRKLFLKLYHPLLLYVNNKHKILKDFQSPEDLSREALE
                     DIEKLKEKLYSKPDQIDSFVRENPQGFSAEE"
BASE COUNT          446 a          447 c          527 g          377 t
ORIGIN      
        1 cgagaatcta cgaccactta agggcggaga tgaaggggga atggaaggaa aaggacactg
       61 aagagcagtt catctataag acccgcaaga aggccttcgg acctttcaag cctgagctgt
      121 ggcagctgcc gactgacgtc cgcgatgaga tctgcaaaga gctggaaggc aaattcgcct
      181 ttttattcga gaagctcatg gttatgggca cccggccagt gctggatcaa tacatcaagc
      241 ctgtggatgt gcctctggaa atgccaaaga tgctgaaggg ctaaagattt atgaccataa
      301 aggccataat atatcgtatc tattagagat aatggtgata aatacgccgt tctcctggga
      361 gcgtcagatg ggattgatat gacaaagatg actataagct tgataaaagc cgatgtgggc
      421 ggctatcccg gccactgctc ggtccatccc gaattgataa agactgcgga ggagaagctg
      481 gaagaggcca aaaagtccgg ctgtctggtg gactacaggg ttatggcctg cggcgacgac
      541 ctggagctgc tcatgagcca caggacgggc tgcgactgcg gcgacatcca cggcctggcc
      601 tgggagacct ttgaattggc cacagagaag gccaaggagc taaagctcta tggctatggc
      661 caggacctgc tggcggacgc cttctccgga aacatcaagg gcatgggtcc aggggtggcg
      721 gagatggaga taaccgagcg cacggcagag cccatcatcg cttttatgat ggacaagacc
      781 gagcccggag ccttcaatct ccccatcttc aagatgtttg ccgatccctt caacaccgcc
      841 gggctgataa tagatccctc attccaccac ggcttttcct tcgaggtctg ggatatccta
      901 gagcacaaga aggtcctcat gaactgcccg gaggagatgt acgatatgct ggcgcttatc
      961 ggtgccaaga gccgatatgt gatcaagaga gtctttgcca agcccggcag caagataccc
     1021 cagagtgagc cggtggcggt gatcagcact gagaagctct accagaccgc tggcaagtat
     1081 gtgggcaagg acgatcccgt ggccttagtg aggtcgcagt ccggcctgcc cgcgctgggc
     1141 gaggtgctcg agcccttcgc cctggggcat ctggtgagcg gatggatgcg gggcagccac
     1201 aacgggccac ttatgccctg ctcatttgat accgctcacc ccaccaggtt cgacggcccg
     1261 cccagggtca tagccgccgg attccagatg gctgagggga agttcgtggg gccggtggat
     1321 ctattcaagg atgtggcctt cgatcgcgtc cggcagaggt gtctggagat caccgattac
     1381 atgagggcgc acggcccctt cgagccgcac cggctgccca tggaggaaat ggagtatacc
     1441 accctgcctc atgtaatgaa ttgcctgaga gatcggttcg tggatacgga gtaattttgt
     1501 tgggacactg cgcatgggtg ttatccgcgg aggagcataa agccaaatat ttgtagcttt
     1561 atgaatctgc catgaacctt ccagatgatg acagaaagct attcctgaag ctgtaccatc
     1621 cactattgct ttatgtaaat aacaaacata aaattctgaa ggactttcag agtcctgagg
     1681 atctctccag ggaggctctg gaggacattg agaagctcaa agagaagctc tactccaagc
     1741 ctgatcagat cgattcattt gtgcgggaga atcctcaggg cttttcagca gaagagc
//
LOCUS       DAOSGP010000284         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_66743_length_1795_cov_14.050000, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000284 DAOSGP010000000
VERSION     DAOSGP010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66743_length_1795_cov_14.050000"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /locus_tag="PLV49_06950"
     CDS             <1..1664
                     /locus_tag="PLV49_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOG98618.1"
                     /translation="PDYVPPKARLVVAIFPFDGEIEITEGADVGELELQDDGTARVLH
                     QPDPSMRAVSDVDILNRRLLFPVKSPAQEGTFRLRCNIYYEQILIQSRLVRAIVANNG
                     LQKEGALRSEVDYALSHTLWADHLRGFAPHRLSILLNSNGDGTHTLSFFGAKEQEPIF
                     KNSAVIPATELKNLTENARKALWKASWGEEKPWKEGEGQTYRYLDRFSDENLSLKRLE
                     EDLISLARRGYLLYDAIIGKFIKTHSKTNELARLMLNPGMVQIAIRDSPTQILPAALI
                     YDYPLDTKAGSYKLCEAFSAALQSNSPLDKAPCFNGLCPHSKRDENDKPDGYVCPSGF
                     WGFRHILGFPTSVPDESASEIVMDDGPHMVVCVATNLDRLDGHMRALREIRTDISWHY
                     SDKRLEIINNLKSPKSHLIYFYCHGGVSANNIPYLQVGDGTGNDFIDGSNLNAFNIEW
                     IEPKPLVFINGCHTTALDPEMAINLVADFVRSGSAGVIGTETTVFEPLACSFAETCLG
                     HFLKDKSSIGDAIRMARLKLLKEYNPLGLVYTPFVLPDLHVKDKG"
BASE COUNT          452 a          452 c          457 g          434 t
ORIGIN      
        1 ttcctgatta tgtgccaccg aaagccaggc tggtggtagc catctttccc ttcgatggtg
       61 agatcgagat cactgaaggg gcggatgtag gggagctgga gcttcaagat gatggaactg
      121 cccgtgtgtt gcaccagccg gatccatcaa tgcgggcagt atcagatgta gatatcctta
      181 accgacgcct gctcttcccg gtcaagtcac cggcacaaga gggcacattc cggttgcgtt
      241 gcaatattta ttacgagcag atcctcatcc aatccagact ggtaagagca atcgtagcaa
      301 ataatggcct gcaaaaagag ggagccctga ggtcggaggt cgattacgcc cttagccaca
      361 ccctatgggc agaccatctc aggggctttg ctcctcatcg cctcagcatt ctcctgaaca
      421 gcaatggcga tggcacccat accctcagct tctttggtgc caaggagcag gagcccattt
      481 tcaagaatag cgctgtgata cctgcgaccg aattgaagaa tctgacggag aatgccagaa
      541 aagccctgtg gaaagcctca tggggggaag aaaagccctg gaaggagggt gaagggcaga
      601 cctatcgcta tcttgatcgc ttttctgatg agaatttaag tctcaagagg cttgaagagg
      661 atctgatatc tctggccaga agagggtatt tgctttatga tgccataatt ggaaagttca
      721 tcaaaaccca cagcaagacc aatgagctgg cgaggttgat gctcaatccc ggcatggtgc
      781 agatcgccat aagggactcg cccacccaga ttctcccggc agctttgatc tatgattatc
      841 ctcttgatac caaagcgggt agctataagc tctgcgaggc tttctctgca gcacttcaga
      901 gcaattcgcc tttggacaag gcaccctgtt ttaatggatt atgcccccac tccaagcgag
      961 atgaaaacga caaaccggat ggctacgtct gtccaagtgg attttggggc ttcaggcata
     1021 tcctgggatt tcccacctct gtgcccgatg aatccgcctc ggagatcgtc atggatgatg
     1081 gtccgcatat ggtggtatgt gtcgccacaa acctcgatcg cctcgatggg catatgaggg
     1141 cgctgcgaga aatcaggacc gatatatcct ggcattattc agataagcgg ctggagatca
     1201 tcaataacct caaaagcccg aaatcgcatc tgatctattt ttactgtcat ggcggggttt
     1261 ctgctaataa tattccctac cttcaggtcg gagatggaac gggcaacgat ttcatagatg
     1321 gatcgaatct gaatgctttc aacatcgaat ggatagaacc caaacctctg gtttttatca
     1381 atggttgcca cactactgcc ctggacccag aaatggcaat taatctggta gccgacttcg
     1441 tccgctcggg cagcgcaggg gtcattggga cagagacaac cgtcttcgag ccgctggcat
     1501 gtagcttcgc cgagacgtgc ctggggcatt ttctcaaaga caaaagctcg atcggagatg
     1561 ccataaggat ggccaggttg aaactgctca aagaatacaa tcctcttggg ctggtttata
     1621 ccccatttgt ccttcccgat cttcatgtaa aagataaggg ctaaatgcca ctggcctgct
     1681 gttgcagtca aagccgacct ttttgatcgg gagacgtact attccctctt ttcagccgct
     1741 ttgagcagac ggatgacatc ctcatcctca agctgtgcaa actcctcgta atgct
//
LOCUS       DAOSGP010000285         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_67987_length_1774_cov_18.394415, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000285 DAOSGP010000000
VERSION     DAOSGP010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67987_length_1774_cov_18.394415"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /gene="gyrA"
                     /locus_tag="PLV49_06955"
     CDS             complement(<1..1682)
                     /gene="gyrA"
                     /locus_tag="PLV49_06955"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG98619.1"
                     /translation="MTEVLVNITEEMKSSYIDYAMSVIVGRALPDVRDGLKPVHRRIL
                     YAMYEQGMTHDQPYKKSARVVGDVMGKYHPHGDAAIYETMVRMAQTFSMRYPLIDGQG
                     NFGSIDGDPAAAMRYTEVRLSRIAEEMLADIEKNTVDFVPNYDGSLKEPTVLPGKLPN
                     LLLNGSTGIAVGMATNIPPHNLNELAGAIIHLIDNPQATVGELMHYLPGPDFPTGGYI
                     VGRAGIESAYMTGKGSIMMQAKSEIEEGAKSTRIIFSELPYQVNKAKLIEDMAEMVKG
                     GRLEGISDLRDESDKDGIRMVVDIKTGFNANVVLNQLHKHTALQTSYGINNMVLVDGM
                     PRILSLKETLEQYIYYRSIVVERRTRFELDAAEKRAHILAGLLIALNNIDEVIRIIKA
                     ASTPAEARDGLIEAFALSEEQAKAILEMRLQRLTGLEQEKIVSEAAELERQIERLRQI
                     LSSNLEILTIIKDELMELISKYGDERRTQIIDAESEVTNEDLIQEEEVAVTITSSGYI
                     KRQPVNTYRMQRRGGRGVIGAETKTEEFVTDVSTASTKDYLLFFTDKGKAHWL"
BASE COUNT          331 a          527 c          481 g          435 t
ORIGIN      
        1 agccagtggg ctttgccctt gtcggtgaag aagagcagat agtccttggt ggaggcggtg
       61 gagacatcgg tcacaaactc ttctgtcttg gtctcggcac ctataacccc ccgcccgccc
      121 cttctttgca tgcggtacgt attgacgggc tggcgcttga tgtacccgga gctggtgatg
      181 gtcacagcca cctcctcctc ctgaatgagg tcctcattag tgacctcgct ctcagcatcg
      241 atgatctggg tacgccgctc gtcgccgtac ttgctgatga gttccatgag ctcatccttg
      301 atgatggtca ggatctccag attgctggag aggatctggc gaagccgctc gatctgccgc
      361 tccagctctg ccgcctcgga gacgatcttc tcctgctcaa ggcccgtcag cctctgcagc
      421 ctcatctcta aaatggcctt cgcctgctcc tcggagagag caaatgcctc tatcagacca
      481 tccctggcct cagcaggggt tgaggctgcc ttgatgatcc ttatcacctc atcgatattg
      541 ttcagggcga tgagcaggcc tgccaggata tgggccctct tctcagccgc atccagctcg
      601 aagcgggtgc gcctttcgac cactatggag cggtagtaga tgtactgctc caaagtctcc
      661 ttcagggaga ggatcctggg catcccgtcc accaggacca tgttgttgat cccgtagctg
      721 gtctgcaaag cggtgtgctt atgcagctgg tttaagacca cattggcgtt gaatccggtc
      781 ttgatatcca ctaccatgcg gatgccatct ttgtccgact catcccgcag atcggatatg
      841 ccctccaggc gtcccccttt gaccatctcc gccatatcct cgatcagctt ggccttgttc
      901 acctggtagg gaagctcgga aaagatgatc ctggtgctct ttgctccctc ctcgatctcc
      961 gacttggcct gcatcattat gcttcccttg ccggtcatat aggcggactc gattcccgcc
     1021 cggcccacga tatatccacc agtgggaaaa tcaggacccg gcaaataatg catcagctcg
     1081 cctactgttg cctggggatt gtctatcaga tggatgatcg ccccggccag ctcattgaga
     1141 ttatggggtg ggatgttggt cgccatgccc acagcgattc cagtcgagcc gttgagcagc
     1201 aggttgggaa gctttcccgg gaggactgtc ggctccttta atgagccgtc gtagttgggc
     1261 acgaagtcca cggtgttctt ctcgatgtct gccagcatct cctcggcgat ccgggatagg
     1321 cggacctcgg tgtacctcat ggctgcagcc ggatctccgt cgatggagcc gaagttgccc
     1381 tggccatcga tcagaggata gcgcatggag aaggtctggg ccattctgac catggtctca
     1441 tatatggccg cgtcaccgtg gggatggtac ttgcccatca catcgcccac cacgcgggcg
     1501 gacttcttgt agggctgatc gtgggtcatg ccctgctcat acatggcata gagaatgcgg
     1561 cggtgaacgg gcttcaatcc gtctcgaaca tcgggcagag ccctccctac gatcacgctc
     1621 atggcgtaat cgatgtaaga ggacttcatc tcctcggtga tgttaaccag tacttctgtc
     1681 acgatccacc tcagatatca agattcttca catacttggc atgcgtctct atgaactccc
     1741 ggcgcggctc cacctggtcg cccatgagga tggt
//
LOCUS       DAOSGP010000286         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_68152_length_1771_cov_19.073427, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000286 DAOSGP010000000
VERSION     DAOSGP010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68152_length_1771_cov_19.073427"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1613
                     /locus_tag="PLV49_06960"
     CDS             75..1613
                     /locus_tag="PLV49_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG98620.1"
                     /translation="MIGFVAAGLLLLLLCTAQADDGGDIQVLTIADYIGDWGYPSPYG
                     HYSRGPGYVRMSLIFDTLVWKDEKGIIPALAESWEMEGDDAYTFKLREDVLWHDGERF
                     AADDVVFTIDYIKEHPYIWVSSDIIKTAEAVDDYTVRISLNQPYAPFLEQVAGTLPIL
                     PEHIYEGVDDPAEFREDSALVGTGPFQLVDYDQSQGSYLYQAYNDYYLGSPKVAQLKF
                     VKVNLEMSAAALEKGDVNAANVPPEVAEDLKDEGFTVMLSSHDSITKIMVNHKKEPLS
                     DSRFRQALYYAIDRQALVDNAARGYGVLASPGLFAVDNHWYNSDVEPYDYDPAKTEEL
                     MEELGYSKVDGYFSVDGSPVKLEMLVTSINERAGELIRQQLEDAGFMISLRTVDSKTL
                     DSMVTEWKFDLALNSHGGMGADPEILKRMTGDGYTFNSARYTENSELNELLDGEVAEI
                     DEQKREDLIDQIQVIHAQELPVLPLYYADTYWAHDGRVDMYYTRQGIANGVPLALNKQ
                     IFVR"
     gene            1638..>1771
                     /locus_tag="PLV49_06965"
     CDS             1638..>1771
                     /locus_tag="PLV49_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG98621.1"
                     /translation="MICGKGGSGKSTVSALIAREMASRGEKVLVVDTDESNFGLYKQL
                     G"
BASE COUNT          430 a          449 c          508 g          384 t
ORIGIN      
        1 tctactgcca aggtgaaatt caaatgctta tcggatcatc aaccagggat tccatctcta
       61 tgagatgcct cccaatgatt ggatttgttg cagctggatt gctgctatta ttgctttgca
      121 ccgcccaggc agacgatggc ggtgatatcc aggtgctgac cattgccgac tatatcggcg
      181 actggggata cccctcgccc tacggccatt actcgcgcgg cccaggatat gtgaggatga
      241 gcctgatctt cgacacattg gtctggaagg acgaaaaggg catcatcccc gctctggcag
      301 agagctggga gatggaggga gatgatgctt atacattcaa gctgcgagag gatgtcctct
      361 ggcatgatgg cgagaggttc gctgccgacg atgtggtctt caccattgat tacattaaag
      421 agcatcccta catatgggtc tcctccgaca tcataaagac ggcagaggcg gtggacgact
      481 atacagtcag aatcagcctc aatcagccgt atgctccttt cttggaacag gttgccggaa
      541 cccttcccat tctcccggag cacatatatg agggcgtaga tgatcccgca gagtttcggg
      601 aggattccgc tcttgtgggc accggcccat tccagcttgt ggactatgac cagtcccagg
      661 gaagctatct ctatcaggcc tacaacgact actacctcgg cagccctaag gtagcacagc
      721 ttaagttcgt caaggtgaac ctggagatgt ctgcggccgc tttggagaaa ggggatgtga
      781 acgcagcaaa cgtgcctcct gaggtggcag aggacctgaa agacgagggc ttcacagtga
      841 tgctctcctc ccatgactct atcaccaaga tcatggttaa tcacaagaag gagcctctat
      901 ccgattcccg tttcaggcag gcgctttact atgccatcga ccgccaggcc ctggtggaca
      961 atgccgctcg cggctacgga gtcctggcca gccccggcct tttcgctgtg gataaccact
     1021 ggtacaattc cgatgtagag ccatatgact acgacccggc aaagacagag gagctgatgg
     1081 aggagctggg ctactccaag gtggacggct acttctctgt ggatgggagt ccggtcaaac
     1141 tggagatgct ggtcacctca atcaatgagc gggcaggaga gctgatacgc cagcagcttg
     1201 aggatgcagg cttcatgata agccttcgca cagtggattc aaagaccctt gacagcatgg
     1261 ttactgaatg gaagttcgat ctggctctaa acagccacgg cggcatgggt gcagatcccg
     1321 agatcctgaa gaggatgacc ggcgatggat acaccttcaa cagcgcccgc tacacagaga
     1381 actcggagct caatgagctg ctggacggag aggtggctga gatagacgag cagaagcgtg
     1441 aggatctgat cgatcagatt caggtgatcc atgcacagga gctgccggtt cttcccctct
     1501 attatgccga tacctactgg gcgcacgacg gcagggtgga tatgtattac accaggcagg
     1561 gaatcgctaa tggcgttcca ctggcactga ataagcagat atttgtccgc tgaaggttgt
     1621 gaatagaaat aaaatatgtg atttgcggca aaggcggaag cggaaagagc accgtctccg
     1681 ctcttatcgc ccgggagatg gcttcgagag gagagaaagt gctggtggtg gacaccgacg
     1741 agtccaattt cggcctctac aagcagctgg g
//
LOCUS       DAOSGP010000287         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_68708_length_1762_cov_16.308143, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000287 DAOSGP010000000
VERSION     DAOSGP010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68708_length_1762_cov_16.308143"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLV49_06970"
     CDS             complement(<1..710)
                     /locus_tag="PLV49_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOG98622.1"
                     /translation="MIELQLDIFQYDFMRVALAAGLASAVLCGIIGIYVILNKIVFIS
                     DGIAHAAFGGIGLGYFLGFDPLIFGVGSAVLTAVGIGMVSGRARVSEDTAIGVFMATG
                     MALGVMLLTLSKGYARDLYGYLFGNILAVTINDVLLISGLTLAIMLLIYILYKEFLIM
                     SFDPVYGEAIGVPVKSLRLLLLAMVAFSVVVLIKIVGIIMVIALLTIPGAISRQHLKG
                     LPSIMAGSAVLGSIFVTVG"
     gene            complement(703..1464)
                     /locus_tag="PLV49_06975"
     CDS             complement(703..1464)
                     /locus_tag="PLV49_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOG98623.1"
                     /translation="MTAEIVSIDNLWIFREEHAILEDINLKLERADFLGLIGPNGGGK
                     STLLKVMLGLIKPDNGRIRIFGMEPEAARARMGYLPQKTIFDQNFPIKVLEVVQMGRF
                     SKTGLFCRYGPADRNAAFRALEEVGMEDRADREIGALSGGEQQRVFVARSLVSDPELL
                     LLDEPTAGVDSSQQTEFYELLCHLNQDMGIAIVLVSHDITAISKYVGKIACLNQRLYY
                     HGSKELTNEDIEKAYGCPVEMIAHGTPHRVLRKHD"
     gene            complement(1461..>1762)
                     /locus_tag="PLV49_06980"
     CDS             complement(1461..>1762)
                     /locus_tag="PLV49_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOG98624.1"
                     /translation="KTFIVFHPAWSYLARDYGLVQVPIMEEEKEPGPQYLASLIDMAK
                     KNDITTIFVDPQFNPKSAEVIAREMNATIVVLDPLAEDYLQNMRRTGEEISKSLK"
BASE COUNT          396 a          503 c          460 g          403 t
ORIGIN      
        1 ccaacagtca cgaatatcga tcccagaact gcggatccgg ccatgatgga gggcagcccc
       61 ttcaggtgct gcctgcttat ggctccggga atggtgagaa gcgctattac cattatgatc
      121 cccacgatct tgatcaatac caccacgctg aaggccacca ttgccaaaag caggagcctg
      181 aggcttttca ccggcactcc tatggcctcc ccgtagaccg gatcgaagct cattatcagg
      241 aattccttgt agaggatata gatcaggagc attatggcca gggtgagccc ggatatcagc
      301 agcacgtcgt tgatggtcac ggctaaaata ttgccgaaaa ggtagccata aaggtctctg
      361 gcatagcctt tggagagggt gaggagcatg actcccaggg ccattccagt ggccataaaa
      421 accccgatgg cggtgtcctc ggatacccgt gccctcccac tcaccattcc tatgccgacc
      481 gcggtcaata cggccgagcc cactccgaat atcaggggat cgaagcccag gaagtagccc
      541 agaccgatgc ccccaaaggc agcatgagct atgccgtcgc ttatgaagac tatcttgttc
      601 aatatgacat agatcccgat tatgccgcag aggacggcgc tcgccaggcc cgcggccagg
      661 gccaccctca taaaatcgta ctggaagatg tccaactgca actcaatcat gcttcctcaa
      721 gacccggtgg ggcgttccat gggcgatcat ctccaccgga cagccgtagg ccttctctat
      781 atcttcgttc gttagctcct ttgagccatg gtaataaagc ctctgattta ggcaggcgat
      841 tttgcccaca tatttggaga tggcagtgat atcgtgcgag acgagcacaa tggctattcc
      901 catgtcctga ttgaggtggc agagaagctc ataaaactcc gtctgctggg aggaatcaac
      961 gcccgccgtg ggctcgtcta agaggaggag ctctggatct gagaccaaag aacgggctac
     1021 aaagaccctc tgctgctctc caccggaaag ggcgccgatc tcccggtcag cccggtcttc
     1081 cattcccact tcctctaggg cgcgaaaggc agcattgcga tcggcaggac cgtaacggca
     1141 gaagaggcct gtcttgctga accttcccat ttggaccacc tccaggacct tgatcgggaa
     1201 gttctgatcg aagatggtct tctggggtag gtagcccatc cttgctcttg ccgcctcagg
     1261 ttccatgccg aatattctga tcctcccgtt atccggcttt atcagaccca gcatcacctt
     1321 gagaagggtg gactttcctc ccccgttcgg gccgattaaa cccaggaagt ccgcccgttc
     1381 cagcttcagg tttatgtcct ccaggatagc atgctcttcc ctgaagatcc agaggttatc
     1441 aatggagacg atctcagcgg tcatttcagg ctcttagaga tctcctcgcc tgtgcggcgc
     1501 atattctgca ggtagtcctc tgccagaggg tcaaggacca caatcgtcgc gttcatctct
     1561 ctcgctatta cttctgctga tttgggattg aactgtggat caacgaatat ggtggtgata
     1621 tcgttcttct tagccatatc gatcaggctt gccaggtact ggggaccggg ctctttctct
     1681 tcctccatga tcggcacctg gaccaggcca tagtcccggg cgaggtagct ccaggcaggg
     1741 tggaaaacga tgaaggtctt at
//
LOCUS       DAOSGP010000288         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_69270_length_1752_cov_19.208014, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000288 DAOSGP010000000
VERSION     DAOSGP010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69270_length_1752_cov_19.208014"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLV49_06985"
     CDS             complement(<1..437)
                     /locus_tag="PLV49_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98625.1"
                     /translation="MKSNQAGAEEESPAFGGQEVTVMAHCLLNPLTRVKGLEPLEFSP
                     AGPTMQLPCPEALYLGTERWAVTRNQLDVPQFRQFCRSLIVHYADLLEMLAKEGVRVR
                     VVGVAGSPSCGVQTTSFGYAGGRVHEAEHEHVPGRGIFMEELIG"
     gene            complement(449..889)
                     /locus_tag="PLV49_06990"
     CDS             complement(449..889)
                     /locus_tag="PLV49_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98626.1"
                     /translation="MNEITNKGEGRGPSPVVPKKLGRIAKKLSAAGEELVLIAPAILH
                     SQASEQMLAIARMGMLDKILGYLVTTDKNVHFVRPGIAWDSVRTVPLDVITGVEYVDE
                     FHTNTLKLLVGERAEKIIFYEDLEGIRFYKYIRGLKRQDQTAEV"
     gene            complement(904..>1752)
                     /locus_tag="PLV49_06995"
     CDS             complement(904..>1752)
                     /locus_tag="PLV49_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase family protein"
                     /protein_id="HOG98627.1"
                     /translation="TLFVPNRSFTSYCGIDAVEDQFTLPLLGSRNLLRSEERGDKRDY
                     YWLLEKAGLPYPEPIEAEDINQLVMVKLPHAVKTLERGFFTAASYEEYCQKADLLLRQ
                     NVIDQDGIELARIERYVIGPVFNLDYFYSPITERIELIGIDWRFETSLDGHCRLPAPQ
                     QLTLNEQQINPEYTVCGHNSATLRESLLEKAFVLAEMYVEATQEYYKPGIIGPFCLQT
                     CVDKDLNFYIYDVAPRIGGGTNVHMAVGHPYGNALWRTNMSTGRRLAREVRIALENDC
                     LDQIVT"
BASE COUNT          349 a          561 c          466 g          376 t
ORIGIN      
        1 ccaatcagct cctccatgaa tatcccccgc ccgggcacat gctcgtgctc cgcctcgtgc
       61 acccgcccgc ccgcatagcc aaagctcgtc gtctgcacgc cgcagctcgg actaccggcc
      121 acccccacca cgcgcacccg cactccctct tttgccagca tctccagtag atctgcatag
      181 tgaacgatca gcgagcggca gaactggcgg aactggggca catccagctg gtttctggtc
      241 accgcccagc gctcggtgcc cagatagagc gcctccggac agggcaattg catcgttggt
      301 cctgcaggcg agaattccag aggctccagg cccttgaccc ttgtgagtgg attcagaagg
      361 cagtgggcca taacggttac ttcctgcccg ccgaaagcag gcgattcttc ctctgccccg
      421 gcttgattgc tcttcatcga tgaccgcctc agacctcagc cgtctggtcc tgtcgcttca
      481 gtccccggat atatttatag aaccttatgc cctccaggtc ctcgtaaaag atgatcttct
      541 ccgccctctc cccaaccaag agcttcaggg tgttggtatg aaactcgtcc acatactcca
      601 caccggtaat cacatccagg ggcaccgtgc gcacgctatc ccaggcgatc cccggccgga
      661 cgaaatgcac attcttatcg gtggtgacga gatatcccag gatcttatcg agcatcccca
      721 tcctggcgat ggccagcatc tgttcagatg cctggctgtg aagaatggcc ggggcgatga
      781 gcaccagctc ctccccggct gcggagagct tcttggctat ccggccgagc ttcttgggaa
      841 caaccgggct cgggcctctg ccctcgcctt tatttgtgat ttcgttcatc ttctccagcg
      901 gcctcaggtc acaatctggt ccagacaatc gttctccaga gcgatcctca cctcgcgagc
      961 gagcctgcgg ccagtggaca tattggtccg ccagagggca ttgccatagg ggtggccgac
     1021 cgccatatgc acattggtcc cacctccgat cctgggcgcg acatcgtaga tgtagaagtt
     1081 caggtccttg tccacacagg tctgcagaca gaaggggccg atgatcccgg gcttgtagta
     1141 ctcctgggtc gcttccacat acatctccgc caggacgaac gccttctcca agagcgactc
     1201 gcgcagggtg gcggagttat gtccacagac ggtgtactca ggattgatct gctgttcatt
     1261 gagtgtcagc tgctgaggcg caggcagtcg gcagtggccg tcaaggctcg tctcaaacct
     1321 ccagtctatg ccgatcagtt caatcctctc cgttatgggg gagtagaagt agtccagatt
     1381 gaagaccggc ccgatgacat agcgctcaat cctggccagc tcaatcccat cctggtcgat
     1441 cacattctgg cgcaagagga gatcagcctt ctggcagtac tcctcatagg aggcagcagt
     1501 gaaaaatccc ctctccaggg tttttaccgc atggggcagc ttgaccataa ccagctggtt
     1561 gatgtcctcc gcttcgatcg gctcaggata tggcagaccc gccttctcca gcaaccagta
     1621 gtagtccctc ttgtcccctc tctcctcgga gcgaagcagg ttgcgggagc cgagcagcgg
     1681 aagcgtaaac tgatcctcca ccgcatctat cccgcaatag gaggtgaatg agcggttggg
     1741 gacgaacagg gt
//
LOCUS       DAOSGP010000289         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_69571_length_1747_cov_14.530733, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000289 DAOSGP010000000
VERSION     DAOSGP010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69571_length_1747_cov_14.530733"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..75)
                     /locus_tag="PLV49_07000"
     tRNA            complement(3..75)
                     /locus_tag="PLV49_07000"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Phe,seq:gaa)
     gene            complement(83..156)
                     /locus_tag="PLV49_07005"
     tRNA            complement(83..156)
                     /locus_tag="PLV49_07005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:Val,seq:gac)
     gene            complement(286..>1747)
                     /gene="carB"
                     /locus_tag="PLV49_07010"
     CDS             complement(286..>1747)
                     /gene="carB"
                     /locus_tag="PLV49_07010"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOG98628.1"
                     /translation="IINNNPETVSTDYDTSDKLFFEPVTLEDVMNIIEKEEPYGLMVQ
                     FGGQTAINLALPLEKEIARLGLKTRILGTSPDSIDLAEDRERFNALMTRLNIPQPNAG
                     IAFSPAEAREVAAKIGYPVLVRPSYVLGGRAMEIVYDEKGLDVYMQEAVRVSREHPVL
                     IDDFLQDAVEIDVDAVCDGTDVLIGAIMEHIEEAGIHSGDSACMIPPQTLPGVVLEVV
                     RDYVRKIALGLGVIGIVNLQMAYKDGTVYVLEANPRSSRTIPFVSKATGLPLAKIAAN
                     VMIGKSLAEQGYTREPKVPYVAVKEVLLPFDKLPGADALLGPEMRSTGEVMGIDYQLG
                     LAFFKAELSAYNPLPLGGVVFISVKDEDKAAAAVAARKLADAGLDIIATDNTAEYLSK
                     SGIKVERVQKIYNGSPNVLDYMKRGEVKLIINTPTTKQSVKDGYQIRRSAVDYHVPYI
                     TTIQAAQAAADAILLAGKDQVTIKALDEYHREVYYT"
BASE COUNT          343 a          565 c          474 g          365 t
ORIGIN      
        1 tatgcccaaa cccggactcg aaccggggac attcagatct tcagtctgac gctctcccaa
       61 ctgagctact tgggctgata ggtgggcccg acgcgattcg aacgcatgac ctccgccatg
      121 tcaaggcgac gtcataacca gctagaccac gggcccgcaa gcaggtttcc tatctttcct
      181 cgcagataaa ggcttcggtc aggcagaagg gggcacgcgg ggagagaatt ctgcagggac
      241 gagaatgcat cttacccgcc atcctgccca actccccaaa ccccctcagg tgtaatagac
      301 ctcccgatga tactcatcca gggccttgat ggtgacctga tccttcccgg cgaggagaat
      361 ggcatcggcc gccgcctgcg ccgcctggat ggtggtgata tagggcacat gatagtccac
      421 cgcactcctc cggatctgat agccgtcttt caccgactgc ttggtggtgg gagtgttgat
      481 tatcagcttc acctcccccc gcttcatata gtccagcaca ttggggctgc cgttatagat
      541 cttctgcacc ctctccacct ttattcccga ttttgagaga tactctgcgg tattgtccgt
      601 ggcgatgata tcaagaccgg catcagcgag cttccgtgcc gccacagccg ccgccgcctt
      661 gtcctcgtcc tttaccgata tgaagaccac tccacccagg ggcaggggat tgtaggcgga
      721 cagctccgcc ttgaagaagg ccaggcccag ctggtagtca atgcccatca cctctcccgt
      781 ggacctcatc tccgggccca ggagggcatc cgccccgggc agcttatcga agggcaggag
      841 aacctccttt accgccacat agggcacctt aggctccctg gtgtacccct gctcggccaa
      901 tgatttgccg atcatcacat tggccgcgat cttggccagg ggcaggccgg tggccttgga
      961 gacgaagggt atggtccggc tggatcgggg gttggcctcc aggacataaa ctgtgccgtc
     1021 cttgtaagcc atctgcaggt tgacgatacc gatcacaccc aggcccaggg caatcttgcg
     1081 cacataatcc cgcaccacct ctaagacgac gccgggcaat gtctggggcg gtatcatgca
     1141 ggcgctgtcg cctgagtgga tgcccgcctc ctcgatatgc tccatgatcg ccccaatcag
     1201 gacatccgtc ccgtcgcaga cggcatccac atctatctcc accgcatcct gcaggaagtc
     1261 gtcgatcaag accggatgct ccctcgagac ccgcaccgcc tcctgcatgt acacgtccag
     1321 tcccttctca tcatagacga tctccatcgc ccggcccccc aggacgtagc tgggccggac
     1381 caggaccgga tagcctatct ttgccgccac ctcccgcgcc tcagccggcg agaaggcgat
     1441 gccagcattg ggctggggaa tgttcagcct ggtcatcagg gcattgaacc tctcccggtc
     1501 ctcagccagg tcgatgctgt cgggcgaggt gcctaaaatt ctcgtcttca gccccaggcg
     1561 agcgatctcc ttttccaggg ggagggccag gttgatggcc gtctggccgc cgaactgcac
     1621 catcaggccg taaggctcct ctttctcaat gatgttcatc acatcctcca gggtgaccgg
     1681 ctcgaagaac agcttgtccg aggtatcata atcggtggag acggtctccg gattgttatt
     1741 gatgata
//
LOCUS       DAOSGP010000290         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_69914_length_1741_cov_20.072361, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000290 DAOSGP010000000
VERSION     DAOSGP010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69914_length_1741_cov_20.072361"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLV49_07015"
     CDS             <1..186
                     /locus_tag="PLV49_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HOG98629.1"
                     /translation="RLAELLRRRRSEIEDLVLVGERLRPLARELGAETAQDLVEGLEK
                     AESSRPKRLLSAVKCFR"
     gene            193..1293
                     /gene="cfbD"
                     /locus_tag="PLV49_07020"
     CDS             193..1293
                     /gene="cfbD"
                     /locus_tag="PLV49_07020"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HOG98630.1"
                     /translation="MASEMQNLNVLHPRPSSIVAALYTLRDLGADVVILHGPSGCCFK
                     HARLLEEDGVRVLTTALDEAGFVFGGHKPLTALLKKAVELFDPQLMAISGTCSSMIIG
                     DDLHQAVLEADLDIPVLEVEVHAGYRDNTKGVIITLEAARDKGIIDEAEFVRQKTLLE
                     KATEVERLRGAASAEYLAPERGDLKYQAAERLLELIRQGKRGLNILNAKKETAYMFAD
                     ITAAVAEVAGEQVDTLANLNDQLGLPKVRRDAVNVASDLEKRGVQFSIIGGLDEYPVT
                     GDIIAQRAQEGGYDFAVVSGVPHALTASALQGLEIFSITNGPRQVKPLRDLGHQHVMV
                     EIDLHPKTMGVTSIVESEFGATLRAMNKAPAK"
     gene            complement(1362..1652)
                     /locus_tag="PLV49_07025"
     CDS             complement(1362..1652)
                     /locus_tag="PLV49_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HOG98631.1"
                     /translation="MSEGNIEAEKKSSARQKPDHKSRLDLKSKLDLDDVDLGVTLPST
                     NINEYIRVLKLARKPTREEFNMIAKVSMAGIGIIGSIGFVIYALLTELPKMF"
BASE COUNT          395 a          440 c          522 g          384 t
ORIGIN      
        1 cgtctcgcgg agcttctcag aaggaggcgc tcggagatcg aggatctggt cttagtggga
       61 gagaggctcc gccccctggc tagagagctg ggggcagaga cggcccaaga cctcgtggag
      121 gggctcgaga aggcagaatc ctctcgccca aaaagattgc ttagtgcggt taagtgtttc
      181 aggtgattaa taatggcaag cgaaatgcaa aatttgaatg tacttcatcc caggccgagc
      241 tcaattgtgg ccgctttata caccctgcgc gacctggggg ctgatgtggt catattgcac
      301 ggcccgagcg gatgctgctt caagcacgcc cggcttttag aggaggatgg ggtgagagtt
      361 ctgaccacag ctttagatga agcgggtttc gtcttcgggg gccacaagcc tctgaccgct
      421 cttctcaaga aagcggtgga gctgtttgat ccccagctga tggccatatc cggaacctgc
      481 agcagcatga tcatcggcga tgacctgcat caggcagttt tagaggcaga cctcgatata
      541 ccggtgctcg aggtggaggt tcatgccgga tacagagata acaccaaagg ggtgatcatc
      601 accctggagg cggcaaggga taaggggata attgatgagg cggagttcgt tcgccagaag
      661 accctgctgg agaaggcgac tgaggtggag aggctccggg gagcggccag cgcagagtac
      721 ctcgctccgg agaggggcga cctgaagtat caggcggcag aaaggctttt ggagctgatt
      781 cggcagggga agcgcggcct gaacatcctc aatgccaaga aggagaccgc ctatatgttt
      841 gcggatatta cggcagccgt ggctgaggtg gctggagagc aggtggatac cctggccaac
      901 ctaaacgatc agctcggcct gcccaaggta aggagggacg ctgtcaatgt cgccagcgat
      961 ttagaaaaaa gaggcgtcca gttcagcatc atcggcggcc tggatgagta tccagttacg
     1021 ggggacatca tcgcccagcg ggcccaggag ggtggctacg actttgccgt cgtgtccggt
     1081 gtgccccatg ccctgaccgc gtctgctctc cagggcctgg agatcttctc cataaccaat
     1141 gggcctcgtc aggtaaagcc cctgcgcgat ctgggccatc agcatgtcat ggtggagatc
     1201 gatctgcatc ccaagaccat gggggtgaca agcatagtag aatcggagtt tggagccacc
     1261 ctccgtgcca tgaataaggc ccctgctaaa taggatggat aaagagataa caaagacaaa
     1321 gcaaagctag tttatggctg ctctccgctg agcagccttt cctaaaacat cttgggaagt
     1381 tctgtgagca atgcgtatat gacaaagcca atggatccaa tgatgcctat gccggccatg
     1441 gataccttgg cgatcatgtt gaactcttct ctggtgggct ttctagccag cttcaggacg
     1501 cgaatgtatt cgtttatgtt agtggagggc aaggtgacgc ccaaatccac atcgtccagg
     1561 tccagcttgc tcttcaggtc cagcctgctc ttgtgatcgg gcttttgcct ggcagagctc
     1621 ttcttctccg cttcgatatt tccctcgctc attctccaga aggaatgaat tggagtataa
     1681 aaaagctttc gatcagctct cttggcttga gccctctatc tccacccggc tctccttttt
     1741 g
//
LOCUS       DAOSGP010000291         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_70842_length_1726_cov_19.676242, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000291 DAOSGP010000000
VERSION     DAOSGP010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70842_length_1726_cov_19.676242"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLV49_07030"
     CDS             complement(<1..776)
                     /locus_tag="PLV49_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG98632.1"
                     /translation="MQIAKKPLLKIEASAESEGIHLSARGPAALLAKPIIDQINKVFA
                     TEKSISSGPDRFIFSTWIPPAPSVAFDRMLNAQVGAMIRRPVPDQFSIAVVKACPNDC
                     LHCSAPSRQGEILSSNVIKGAISQALDMGSYLVTFDGGEPMLRKDLPDLVSSVDQRAI
                     ATSFTSGYHLTAELAKQLKDAGLYAVRISIDSPIEGQHDRFRGRKGAFQDALFGVRNA
                     LEAGLLVDLFMVTSPHNIDYLEDAFSLAAELGVQELSLYE"
     gene            complement(749..1684)
                     /locus_tag="PLV49_07035"
     CDS             complement(749..1684)
                     /locus_tag="PLV49_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG98633.1"
                     /translation="MFSARDEYHLINKELAALVRDLPDSPLGEILSYVLSAPGKRVRP
                     LILILSAEALGTPPAQSISAALAIELVHAASLVHDDILDHGIERRGSPSALERFGPEA
                     SLLAGDWLISRSIDLISGYSQPVTRAFSRACMDMSEGELMDLSCAGSPEDYYQCITRK
                     TASLFSASAKIGGLISGAGEEDVARLESYGLHLGLGYQVLDDLEEFLGLDQGKSSQKT
                     SVTLPKIYASRSSRSASHDTDSDAGFDADSDIGSDTANVVQMCIKVTDDHCNQAKGAL
                     QALNGDEEKKLQLLEIVDEMTCRGLQRCRSQKSLC"
BASE COUNT          378 a          502 c          494 g          352 t
ORIGIN      
        1 atctcgtaga gggatagctc ttgcaccccc agctctgcag ccagactgaa tgcatcctcc
       61 agataatcga tattatgagg ggaggtcacc atgaacaggt ccaccaaaag gcccgcctcc
      121 agggcattgc gaaccccgaa taaggcatcc tgaaaagccc ctttgcgccc ccggaagcgg
      181 tcatgctgcc cctcgatggg gctgtcgatg ctaatccgta ccgcatagag ccccgcatct
      241 ttcaattgct tcgcaagctc agctgtcagg tggtagccgc tggtgaagct ggtggcaatt
      301 gcccgctggt cgaccgaaga gactaggtcg ggcagatcct tgcggagcat gggctctcca
      361 ccgtcgaagg tcaccaggta cgagcccata tccagggctt gggagatcgc ccccttgatc
      421 acgttgcttg agagtatctc cccctgacgg gagggggcgg aacagtgcag gcaatcgttg
      481 gggcaggcct tcactaccgc aatggagaac tggtcgggca ccggccggcg gatcatcgcc
      541 cccacctgag cattcagcat gcggtcaaag gcaacgctcg gagcaggagg aatccaggtg
      601 gaaaagatga acctgtccgg gccggagctg atggattttt ccgttgcaaa gaccttgttg
      661 atctggtcga ttatcggctt tgccagaagg gcagccgggc cgcgggcaga aagatgaatt
      721 ccttcagact ccgccgaggc ctcgatcttc agcagaggct tttttgcgat ctgcatcttt
      781 gcagtccccg gcaggtcatc tcatccacaa tctccaggag ctggagcttt ttctcttcat
      841 ccccattcaa ggcctggagg gcacctttgg cctggttgca gtgatcatcg gtgactttta
      901 tgcacatctg caccacgttg gcggtatcag atccaatgtc cgaatcagca tcaaaaccag
      961 catcagaatc ggtatcatga gaagctgaac gagagcttcg gctggcatag atcttgggaa
     1021 gggtgaccga ggtcttctgg gaggacttcc cctgatccag gcccaggaac tcctccaggt
     1081 catcgaggac ctgatagccc aggcccagat gcagcccata gctctccagc ctggccacat
     1141 cctcttcccc tgccccgctg atcagcccac cgatcttggc tgaggcggag aagagggatg
     1201 ccgtctttct ggtgatgcac tgatagtaat cctcaggcga gcccgcgcag gagaggtcca
     1261 tcagttctcc ctcggacatg tccatgcagg cgcgagagaa cgcccttgtc accggctggc
     1321 tatagccgga tatcaggtcg atcgacctgg agatgagcca gtcgcccgcc aggagcgaag
     1381 cctcaggacc aaaacgctcc agagcactgg gcgagcccct tctctctatg ccatgatcca
     1441 gaatatcgtc atgcaccagg gatgcagcat ggaccagctc aatggctaaa gcagcgctta
     1501 tggactgggc aggaggggtg ccgagagcct ctgcagagag aataagaatg agcggcctga
     1561 ccctctttcc cggcgcggaa agcacatagc tcagtatctc ccccagaggg gaatcgggca
     1621 ggtccctcac cagtgcggca agctctttat tgatgagatg gtactcatcc cgggcggaga
     1681 acatcgatcc aagtagtctc ggccaggtta tttagctttt gctgag
//
LOCUS       DAOSGP010000292         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_70843_length_1726_cov_18.996409, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000292 DAOSGP010000000
VERSION     DAOSGP010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70843_length_1726_cov_18.996409"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PLV49_07040"
     CDS             <1..1071
                     /locus_tag="PLV49_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HOG98634.1"
                     /translation="VPPRLMTPAMSIHNILEKFKRYTMIEKVIFMGAVASCFPPQGST
                     GVEVVANEMEGELATAGIKEGSRWTDVDFRNPCLSMDFGTTLDGRVTSEELPYAHTIG
                     NLLGLAGAIPDAVVQGTGLVDRKIGATLDIFDAGLKPDYGKEAQAYADRIDELVIIEK
                     VPLNRKKYGLVPVNPDAAAKNNVVLIGCDVGVNGSDLEKLSGIGADINSTKSRDLKVL
                     FGALDLAMARVARRLVQVGVEEGIVTGKTAIGVTGRAGITGNKPRLILEEIDKLGLYD
                     HTDQNVVFVDDGLARGAAVMARCMNSMGTPRNPLGGLRKGGCILKKRMSYEVEKGLVP
                     APQEARPDKDTHDYFEGGHRRE"
     gene            1090..>1726
                     /locus_tag="PLV49_07045"
     CDS             1090..>1726
                     /locus_tag="PLV49_07045"
                     /inference="COORDINATES: protein motif:HMM:NF016030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98635.1"
                     /translation="MPPPPRSHPPARVEEEIPISVLAIEEQEETIWISLNAISASEFP
                     PCIKNIIRGAGGEKGMHRRGAILASFLGQCGWSEKEAGSLWRSAISAEERIYRKWFGN
                     MNCPKCETMQRQSKGYPDLGIEDLELCQPDEKCQRIKSPVEYAADLFAEEELSRGRQE
                     HIRTIFLARLFNWKTGKEFDIDLTAEEKEDLESLKNKQMETDSANTILIFTW"
BASE COUNT          451 a          428 c          541 g          306 t
ORIGIN      
        1 gtccctcccc ggctcatgac cccggcgatg agcatacaca acatcctgga gaagttcaag
       61 agatatacca tgatcgagaa ggtcatcttc atgggagcag tggcctcctg tttcccgccc
      121 caggggtcca ccggagtaga ggtggtggcc aacgagatgg aaggagagct tgccaccgcc
      181 ggaatcaagg agggcagccg ctggacagac gtggactttc gaaacccctg cctctccatg
      241 gacttcggca ccaccctgga cggcagggtg acgagcgaag agctgcccta tgcccatacc
      301 attggcaacc tactgggcct agctggtgcc atacctgatg ccgtggtcca gggcacaggg
      361 ctggtggaca ggaagatagg ggcgaccctg gacatattcg acgctggcct gaagcccgac
      421 tatggaaagg aggcccaggc ctatgccgac cggatcgacg agctggtgat catcgaaaag
      481 gttccattaa atcgcaaaaa atacggcctc gttccggtga atccagatgc agcggccaag
      541 aacaatgtgg tccttatcgg ctgcgacgtg ggggtaaacg gcagcgacct ggagaagctc
      601 tccggaatcg gcgctgatat caatagcact aaaagccgag atctgaaggt actctttggt
      661 gctctggacc tggccatggc ccgggtggcc cgcaggctgg tccaggttgg ggtcgaggag
      721 ggcatagtca ccggcaagac cgccattggt gtgaccggta gggcgggtat cacaggcaac
      781 aagccccgcc tgatcttaga ggagatagat aagctcgggc tgtatgatca tacggaccaa
      841 aatgtggtct tcgtggacga tggcctggcc agaggagcag cggtgatggc tcgatgcatg
      901 aactccatgg gcactcccag aaatccgtta ggagggctgc gcaaaggagg gtgcattctc
      961 aagaagagga tgagctatga ggtggaaaaa gggctggtac ctgcgcccca ggaggcccgg
     1021 ccggataagg atacccacga ttacttcgag ggaggccaca ggcgagaatg agaagagatt
     1081 gagcagaaaa tgccaccacc ccccagatca catcctccgg ccagggtcga ggaggagatt
     1141 cccatatccg tgctggcaat agaagagcaa gaggagacga tctggatctc cttaaatgca
     1201 atttcagcca gcgaattccc tccttgcata aagaatataa ttagaggggc tggaggcgaa
     1261 aaggggatgc acaggagagg agcgatcctg gcctcattcc tgggccaatg cggttggagc
     1321 gaaaaggagg caggatccct ctggcggagt gctatatctg cagaggagag gatctacagg
     1381 aaatggtttg ggaatatgaa ctgcccaaag tgcgagacca tgcagcggca gagcaagggc
     1441 tatcccgacc tgggcattga ggatcttgag ctatgccagc cggatgagaa gtgccagagg
     1501 ataaaaagcc cggtggagta tgctgcagat ctgtttgccg aagaggagct ctccagaggg
     1561 agacaggagc acattcgaac tatatttctg gcccgacttt tcaactggaa gaccggtaaa
     1621 gagttcgata tcgacctgac ggcagaggag aaagaggatc tggagagcct gaaaaataaa
     1681 caaatggaga cggatagcgc caacaccatc ctcatcttca cctgga
//
LOCUS       DAOSGP010000293         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_71677_length_1712_cov_14.883524, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000293 DAOSGP010000000
VERSION     DAOSGP010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71677_length_1712_cov_14.883524"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            353..1234
                     /gene="modA"
                     /locus_tag="PLV49_07050"
     CDS             353..1234
                     /gene="modA"
                     /locus_tag="PLV49_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG98636.1"
                     /translation="MNERMTSMILVAMLLAGTILSVTMAMEASDLGSQTAPNEELVIF
                     CGAGFTGAMSEIAELYENSSNSQVRFNFDGLPALRVQIEEGAYADLLISPDQRYMDPL
                     VDAGFIGEDTVEAFASNKVAVVLPVDNPANIITIRDLATPGTKIVMGIEELPIGDYAL
                     QVLDLMAKDPEYGPAFKEAVLANVVSLETRVTGIVSKVAMGEADAGFAFMSDVTPQMR
                     GKIRKISIPEGYNIRGNCSAAVLSQSDNSEAAEAFIDLLISNDAQAILDEYGFIPLEG
                     WEENAALPAGDRAAAID"
     gene            1339..>1712
                     /locus_tag="PLV49_07055"
     CDS             1339..>1712
                     /locus_tag="PLV49_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98637.1"
                     /translation="MTGGELLEETKRRFIQLLKFQGDGVYRRELQSEVVISRPLSVAE
                     AIGDPGRDDFPIIRGREVLMQATFKGVAGQAFTAASGHFRGTLGDVLALPLDENFERA
                     VFVATMNAALRYLGKIEKTVHCR"
BASE COUNT          407 a          407 c          500 g          398 t
ORIGIN      
        1 tactgagtaa aaacgtacat tatatttata gattatgcat tgctggcctt atggatccat
       61 agtccggagg ttctgccttg gatctcaacc attttctcgt ccctgtcctg tcatccagag
      121 agtatcaggc tcttcgctga attatgcggt tgaggtccga cagaggttat agcctctatt
      181 cctctgcgtc tctgtgttgg gtcagagcat agcggttcgc catagaggtg aaagatgcag
      241 agaatttccg attcaaaatt cacccaccaa atccggagat cagcgatctc tgccgcaaag
      301 gggataatcc agtagtccgc attccggatc tcaaatgaaa gcgggtggat ctatgaatga
      361 gagaatgact tcaatgatat tggtggcgat gttattggct gggacgatac tgtccgtgac
      421 catggctatg gaagcatcgg atctcggctc tcagaccgct ccaaacgagg agctggtaat
      481 cttttgcggt gcaggattta ccggagctat gagcgagatc gccgagctct atgagaatag
      541 cagcaattct caggtgcgat tcaacttcga tggccttccc gccctccggg ttcagatcga
      601 ggagggtgcc tatgccgatc ttttgatatc gccggatcag aggtacatgg atccattggt
      661 cgatgcaggc tttattgggg aggacacagt cgaggctttt gccagcaata aggtggcagt
      721 ggtcctcccg gtggacaatc ctgccaatat catcaccatc cgggacctgg ccactcccgg
      781 gacgaagatc gtgatgggaa tagaagagct tcccataggg gactatgccc tgcaggtcct
      841 tgaccttatg gcaaaagatc ccgaatacgg cccggccttc aaagaggcgg tcctcgccaa
      901 tgtggtatct ttagagacaa gggtcacggg catagtctcc aaggtcgcca tgggggaggc
      961 ggatgccggc ttcgccttca tgtccgatgt cacccctcag atgagaggaa agatcagaaa
     1021 gatctccatt ccggagggat acaatatacg gggaaactgc agcgctgctg tgctctcgca
     1081 atccgataat tctgaggctg cagaggcatt catcgatcta ttgatatcaa atgatgctca
     1141 ggccatatta gacgaatatg gattcattcc cctggagggc tgggaagaga acgcagcctt
     1201 gccggccggg gaccgggccg cggccatcga ttaatgccga gaggatccaa atcgcatgac
     1261 ctgatggggc gttgaggctc aaagatatta tgaggctata aagcacgggg ataccatcag
     1321 ctttaagagg ggattactat gaccggcgga gaattgttag aggagacaaa gaggcgtttc
     1381 attcagctgc tcaagttcca gggcgatggg gtatatcgcc gggagctgca aagcgaggtg
     1441 gtcatcagcc ggccgctcag cgtggcggag gccatcgggg atccgggaag ggacgatttt
     1501 cccataatca ggggacgaga ggttctcatg caggccacat ttaagggagt tgccgggcag
     1561 gcgttcactg ctgctagtgg gcatttccgg ggaactctag gagatgtcct ggccctgccc
     1621 ctggatgaga acttcgagcg ggcggtcttt gttgccacca tgaatgctgc tctcagatat
     1681 ctgggcaaga tcgagaagac agttcactgc cg
//
LOCUS       DAOSGP010000294         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_71791_length_1710_cov_20.213897, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000294 DAOSGP010000000
VERSION     DAOSGP010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71791_length_1710_cov_20.213897"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /gene="cbiQ"
                     /locus_tag="PLV49_07060"
     CDS             <1..864
                     /gene="cbiQ"
                     /locus_tag="PLV49_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOG98638.1"
                     /translation="FVGKTIDGIFGFMEEAFVSEASSRLPGVLQSLDPRAKLISILAI
                     VFAASLIGDLNVLVFIYLLTLLFAYLSKIDVLFFIKRVWLFIPVFAGIIALPMIFNIF
                     IPGDPLIHLANLGPGAHLGPFSLPESIYITRQGTVAAVIFTMRVAACVSAVVLLFITT
                     SKQVLFKSLRSVGVPKLYVLTLEMAYRYIFLLMELVREMYIAKKARTIRAGGLFDEQK
                     WVGGRMGYTLIRSLDMSEKVHMAMTSRGFNGEVHIMQEFKFRSRDYLAGATAISLGIL
                     LLLISQNIPRI"
     gene            869..>1710
                     /locus_tag="PLV49_07065"
     CDS             869..>1710
                     /locus_tag="PLV49_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG98639.1"
                     /translation="MEKKMDAIFDLRNISYSYLGKIEALKDITLKINRGEEVTIIGSN
                     GSGKSTLLAVLNGLVYPTSGEFYAFGHPVTEEVFDSIKENEFRTYFRTKVGFVFQNSD
                     IQLFSSTVFEEIAFGPLQLNIPLEEVKARVEDIIEMMQIAKLRDRAPHTLSGGEKKKV
                     CIATVLVNNPDVLLLDEPSAGLDPRTQLWLIELLSELSHAGKTIITATHDLEIMEMIS
                     KRSIVMGEDHRIKLDDTPKRVLSNYELLMESNLVHEHMHIHGKLVHEHLHDHDAGHTH
                     VHLK"
BASE COUNT          416 a          443 c          442 g          409 t
ORIGIN      
        1 tttgtgggaa agacgataga cgggatattc ggctttatgg aggaggcatt cgtctctgag
       61 gccagttccc gcctgcccgg cgtcctgcag agcctggacc caagggcaaa gctgatctcc
      121 attctggcca tcgtatttgc agccagcctg attggagatc tgaatgtcct ggtattcatc
      181 tatctcctca cgctgctctt cgcatatctg agcaaaatag atgtgctctt tttcatcaag
      241 agggtatggc ttttcatacc ggttttcgct gggatcattg ccctgcccat gatcttcaat
      301 atcttcattc cgggcgaccc cctcattcac ctggcaaacc tcggaccggg agctcacctg
      361 gggccgttct cccttccaga atccatctac ataacccgcc agggaacggt tgccgccgtg
      421 atcttcacca tgagggtagc agcatgcgtc tcggcagtag tattgctttt tatcaccacc
      481 tccaagcagg tcctcttcaa atccctgaga tctgtcggcg ttccaaagct gtatgtcctc
      541 accctggaga tggcctatcg ctatatcttc ctcctgatgg agctggttcg agagatgtac
      601 atcgccaaga aagcaaggac catccgcgca ggagggctgt tcgacgagca gaagtgggtg
      661 ggagggagaa tgggctatac cctgatcaga tccctggaca tgagcgagaa ggtccatatg
      721 gccatgacct cccgaggctt taacggcgag gtgcatatta tgcaggagtt caaattcagg
      781 agtcgtgact atctcgccgg ggcaacagct atatctctgg gcattctgct ccttctaatc
      841 tctcagaata tccctcggat ataggagcat ggaaaagaag atggatgcca tatttgacct
      901 caggaatata tcctattcct acctgggaaa gatcgaggcc ctaaaggata tcactcttaa
      961 gatcaatcga ggggaagagg tcacaataat cggctcaaac ggcagcggaa agtctaccct
     1021 cttagccgtc ttgaacggcc tggtataccc gacatcgggc gaattttatg ccttcggcca
     1081 tccggtaacc gaggaggttt tcgactccat aaaggagaat gagttcagga cctacttcag
     1141 gacaaaggtg ggctttgtct tccagaactc cgacatccag cttttctcct ctacggtctt
     1201 cgaggagatt gcctttggcc ccctgcagct gaatatacct ttggaggagg tgaaagccag
     1261 ggttgaggat ataatagaga tgatgcagat cgcaaagctg cgggacagag cgcctcacac
     1321 cctgagcgga ggggagaaga agaaggtctg catagccacc gtcttagtga acaatcccga
     1381 tgtgctgcta ttggacgaac cctctgcggg ccttgatccc aggacccagc tttggctgat
     1441 agaacttctc agcgagctat ctcatgccgg gaagaccatc atcactgcta cacacgacct
     1501 ggagatcatg gagatgatca gcaaaaggtc cattgtgatg ggtgaggatc acaggatcaa
     1561 gctggatgac actccaaaga gagttctctc caattatgag ctccttatgg aatccaatct
     1621 ggtgcatgag cacatgcata tccacggcaa gctggtacat gagcacctgc acgaccatga
     1681 tgcggggcat actcatgttc atctgaagag
//
LOCUS       DAOSGP010000295         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_72135_length_1705_cov_13.120000, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000295 DAOSGP010000000
VERSION     DAOSGP010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72135_length_1705_cov_13.120000"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..816
                     /locus_tag="PLV49_07070"
     CDS             325..816
                     /locus_tag="PLV49_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOG98640.1"
                     /translation="MELRLEPVRTEDLPAIRDIFNYYIENSFAAYLEMPVPLEFFGTF
                     LRMVEGYPFLAAKDSDGRLLGFGLLRPYNTMPAFSRTAEITYFVSPEFTRHGIGGMLL
                     DRLLAKAREMGITSILASISSLNPPSLSFHESHGFEECGRFAEIGRKKGQGFDVVWMQ
                     KMV"
     gene            complement(882..1400)
                     /locus_tag="PLV49_07075"
     CDS             complement(882..1400)
                     /locus_tag="PLV49_07075"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOG98641.1"
                     /translation="MRDWREDDAASIAQHANNKRIWINLRDAFPHPYSLTDAEAFLSK
                     AMRMEPITYFAIASDQEAIGSIGLMPGDDVHRLTAELGYWLAEPFWGNGIMTKVVRAL
                     SEYAFRELGLLRIFAEPYTTNAASARVLENSGFTLEGVIRASVFKEGRILDQYLYAKL
                     NKRALQQSTTPP"
     gene            complement(1503..>1705)
                     /locus_tag="PLV49_07080"
     CDS             complement(1503..>1705)
                     /locus_tag="PLV49_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOG98642.1"
                     /translation="VHPSGEEEVRSCAQFSSDRVVVDGNIITSRTPGTAMEFALKLVE
                     VFLGKKKMEQVKAQTLAICKGD"
BASE COUNT          383 a          447 c          435 g          440 t
ORIGIN      
        1 ttgtggttta tggctcttcg ctgaattatt tgggtgaggt acggcagcgg ttataactct
       61 gttcctctgt ggttgggtca aagcatagcg gttcaccgca gaggtgcaaa gatacagaaa
      121 atttgcgatt caagttcacc cctactacat agcgaggagc cgagtctctt tctcgaatct
      181 ggatcacagg ctggattctc tgtgcagcca ggaaaaagag gcattactgc agccctggtg
      241 attcgtgccg caggagccca caggccaata gagggataga attaaattct ggaccgtggc
      301 agaggctcgg caggaggtcg gttaatggag ttgaggctgg agccggttcg cacggaagat
      361 ttgcccgcga ttagagatat cttcaattac tacattgaga acagctttgc cgcttatctg
      421 gagatgcctg ttcctctgga gttcttcggc acgttcctga ggatggttga aggatatcct
      481 ttcctggcag ctaaggacag cgacggccgc ttgctcggtt ttgggctttt gcgtccctat
      541 aataccatgc ctgccttttc aagaacggct gagatcacat acttcgtctc tccagaattc
      601 acgcgtcatg gcattggagg aatgctcctg gaccgcctcc tggccaaggc ccgtgaaatg
      661 gggatcacat ccatactggc gagcatatcc tcgctcaatc cgccaagcct ttccttccat
      721 gagagtcatg gattcgaaga gtgcgggcgc tttgctgaga ttgggcggaa gaaggggcag
      781 gggttcgacg tggtgtggat gcagaagatg gtctaatacc actggaaatg cagatgacgt
      841 caagcttcat acaatatcaa cgtagtcgca agccccgtcc ttcagggcgg ggtagttgac
      901 tgttgcagtg cgcgtttgtt cagtttagcg tacaagtact ggtcgaggat tcttccctcc
      961 ttgaagacgc tagccctgat aactccttca agggtaaaac ctgaattctc aagcaccctg
     1021 gctgaagcag catttgtagt gtaaggctct gcaaatatac ggagaagtcc gagctctcta
     1081 aaagcgtatt cgctcagtgc tcttaccact tttgtcatga tcccattgcc ccagaaaggc
     1141 tctgccagcc aatatcctag ctcggcagtc aatcgatgaa catcgtcccc aggcatcaag
     1201 ccgatgctgc caatggcctc ctgatcggat gcaatcgcaa aatacgttat gggctccatg
     1261 cgcatcgctt tagagaggaa cgcttctgcg tccgtcagtg agtagggatg gggaaaagca
     1321 tctcgaagat tgatccaaat cctcttgttg ttcgcatgct gcgctattga tgcagcatcg
     1381 tcctcgcgcc aatctcgaat ggtgtacttc ccaattgcga tatgcctgat agatatcctc
     1441 ctcctcgctt ttcaaatccg ctctcgccgg ttgctctgag agcttcacca ttgctcttca
     1501 tttcaatccc ctttgcatat cgccagggtt tgagccttga cctgctccat cttcttcttg
     1561 cccaagaata cctctaccag cttcaaggcg aactccatcg ccgttccggg agtgcgactg
     1621 gttatgatat ttccatcaac caccacccgg tcactggaga actgggcgca cgaccttacc
     1681 tcctcctccc cagaaggatg cacag
//
LOCUS       DAOSGP010000296         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_72322_length_1702_cov_21.360049, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000296 DAOSGP010000000
VERSION     DAOSGP010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72322_length_1702_cov_21.360049"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..85)
                     /locus_tag="PLV49_07085"
     tRNA            complement(11..85)
                     /locus_tag="PLV49_07085"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Glu,seq:ttc)
     gene            complement(180..252)
                     /locus_tag="PLV49_07090"
     tRNA            complement(180..252)
                     /locus_tag="PLV49_07090"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:Gln,seq:ttg)
     gene            357..1355
                     /locus_tag="PLV49_07095"
     CDS             357..1355
                     /locus_tag="PLV49_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOG98643.1"
                     /translation="MKLLTKNHMSQRRLIEILRVIESAGIPVGARAISDILCSRGYDL
                     GERAVRYNLKILDELGFTRKKGYSGRIITPLGSRELSDALIDDRIGFVNTRIEEYMYK
                     SNFDPCSGQGQVIANTSIVDKADAEEVLDMLGRAFDEGYTISRRALILDEGDAISTLK
                     VPAGSLGLATVCSITMDGILMKKGIAVLTSFAGLAKIKEKQPIEFTDLIAYAGSSLDP
                     VKVFMGRKVTSVANAIRLGSGRVLANVREIPVAAAGHALELLEASRSAGFGGLIKVGG
                     PAEPILGCPVAAGKIGIAFYAGVNGAVAAEEMGARMKTLPISMLVDYSRMTDLDRP"
     gene            1451..>1702
                     /locus_tag="PLV49_07100"
     CDS             1451..>1702
                     /locus_tag="PLV49_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6e family ribosomal protein"
                     /protein_id="HOG98644.1"
                     /translation="MDFRVVVSDPKSGKAYQVELKDPGAGKLLGKHIGDKIEGDILGM
                     PGYSMQVTGGSDREGFPMRADLPGTKRRKILLSTGTGYHP"
BASE COUNT          410 a          414 c          506 g          372 t
ORIGIN      
        1 tatggtaaaa tgctccggcc gggatttgga cccgagtctt cggcttgaaa ggccgaaatg
       61 attggccggg ctacactacc ggagcaactg gtgacgggcg atataatcaa tcagcctggc
      121 cctctcccca tgagatcgac cagatatatc tacatttctg atggcttggg gattttgagt
      181 agtcccgggc ggattcgaac cgccgtcgcg ggctccaaag gccctcagga ttgacctcta
      241 ccccacggga ctatatcttt tagcctctac tagccgccaa ggccattgct cttggaggct
      301 ttggcccttg ttctccgcat aagatatata ttccctttcc cggaggatct gggtagatga
      361 agctgctcac caagaatcat atgagccagc gcaggctgat agagatcctc agggtgatcg
      421 agagcgccgg catacctgtg ggggcgaggg cgatctcaga catcctttgc agccgtggct
      481 atgatctggg tgagagagcg gtgcgctaca acctcaagat cctggatgaa ctgggattca
      541 ccaggaagaa ggggtacagt ggtcggatta ttacccctct gggctccagg gagttgagcg
      601 atgcgctcat agacgaccga attggatttg tgaataccag gattgaagag tatatgtata
      661 agagcaactt cgatccctgc agcggccagg gccaggtcat cgccaatacc agcatagtgg
      721 ataaggcaga tgctgaggag gtcttggata tgctcggtcg cgccttcgac gagggctata
      781 cgataagccg ccgtgcattg atcctggacg aaggagatgc gatttccacc ctaaaggtgc
      841 ctgccggatc cctgggattg gctacggtat gcagcataac catggatgga atcctgatga
      901 agaagggaat tgctgtcctc accagcttcg ctggcctggc aaagatcaag gaaaaacagc
      961 caattgagtt caccgacctc atagcttatg ccggatcctc cctggacccg gtgaaggttt
     1021 tcatggggag gaaggtgacg agcgttgcca atgccatccg cttgggttcg ggaagagtgc
     1081 tggccaatgt tcgggaaatt cccgtcgcgg cggctggcca tgctctggag ctgctggagg
     1141 catcccgatc cgcgggcttt ggcggcctca taaaggtagg aggtcctgct gagccgatac
     1201 tgggctgccc tgtagctgcg ggaaaaatcg gcatagcctt ttacgctggt gttaatgggg
     1261 cggtggccgc ggaggagatg ggggcgagaa tgaagacact gcccatatcc atgctcgtgg
     1321 actattcacg gatgaccgat ctggacagac cttgaatgaa gcccatcatt taaagctaaa
     1381 agacaagata aggttttata gggcattgat ccctagactc aaagtcacat ttcattgatg
     1441 aggaagaacg atggatttta gagttgtagt ctcagatccc aagagcggaa aagcctatca
     1501 ggtagagctt aaggatccgg gagcaggcaa gcttctgggg aagcatatcg gcgacaagat
     1561 agaaggagat atattgggca tgccaggata ctccatgcag gtcacaggcg gcagcgatcg
     1621 cgagggattt cccatgcgtg cagacctgcc gggcaccaag agaaggaaga tcctcctctc
     1681 cactggaact ggctatcatc cc
//
LOCUS       DAOSGP010000297         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_72971_length_1692_cov_15.689676, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000297 DAOSGP010000000
VERSION     DAOSGP010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72971_length_1692_cov_15.689676"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PLV49_07105"
     CDS             <1..1286
                     /locus_tag="PLV49_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98645.1"
                     /translation="DEFALVKNNLAIAYLSLAGAGDERDKTECCRQSIQSCRDALLIR
                     TRENQPLAYASSQNNLGNAFLTLAEEEASLENCELALEAFRNALDLYPREQHPTLYAA
                     TQNCIGNAHLALAQIQNPAENFLKAIGAARKALEIFNRESNPEEYAESQGILWLGYLT
                     LADIEYRAENCLLALEACQERLIAFSQGKPLQYASCQKDLAITYSMLADTEVSVEAKA
                     EDCRKGAEAALEALSIYNESDHPIEHAEAQLLLWAAYSALAEVEEKEENCRKAIDACQ
                     AAVRIYEASHPTEHADALKSLGYSFITLAEVTDKAENCRRAIEACSASLEHYQKDEAP
                     REHAEILRDLAFAYVTISEVEDREEWVKRALRAYKKATRIFEGLAQELEAERDPEAAE
                     MRNDVQRCRRSMQSCKGILKASRKRRSEVQPLESR"
     gene            1288..>1692
                     /locus_tag="PLV49_07110"
     CDS             1288..>1692
                     /locus_tag="PLV49_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HOG98646.1"
                     /translation="MKDLAESDKMEYFRRSYRSVDGLWFMMIEKKRGFDEALELDEAV
                     WRVLPKIQARAIKSMLMLDYGLDGLKEAIAARLVLEEFDYELKEEEDGFAVEIRRCPW
                     HDIMVRSGRESLSEKVSEVICQAENSVWAQEFS"
BASE COUNT          434 a          428 c          507 g          323 t
ORIGIN      
        1 cggatgagtt cgccctggtc aaaaacaacc tggccatagc ctatctgtcc ctggcagggg
       61 caggggatga aagggataag acagagtgct gcaggcagtc tattcagtca tgccgcgacg
      121 ctcttctcat ccgaacccgg gaaaaccagc cgctggcata tgcatcatcc cagaacaatc
      181 tgggaaatgc cttcctcacc ctggccgagg aggaggcgag cctggagaac tgcgagctcg
      241 ctttagaggc cttcaggaac gccctggatc tctacccccg ggagcagcat ccaacgctat
      301 atgctgccac tcaaaattgc attggaaacg ctcacttggc actggcccag attcagaatc
      361 ccgccgagaa cttcctgaag gctattggtg ctgctcgcaa ggccctggag atattcaata
      421 gagagagcaa ccctgaggag tatgcagaat cgcaggggat actctggctt ggctacctca
      481 ccctggcgga tatagaatac cgggctgaaa actgtctttt agccctggag gcctgccagg
      541 aaaggctgat agccttttcc cagggcaaac ccctgcaata tgcctcctgc caaaaagacc
      601 tggccatcac ctacagcatg cttgccgaca cggaggtctc tgtcgaggcc aaagcagagg
      661 attgccggaa aggagcagaa gcggcattag aggcgcttag catctacaat gaatctgatc
      721 atccaattga gcatgctgag gcacagctcc tcctatgggc ggcatactct gccctggctg
      781 aggtcgagga aaaggaggag aactgccgca aggctattga tgcctgccag gccgccgtcc
      841 ggatctatga agcatcccat ccgacagagc atgcagatgc tctgaagagc ctgggctaca
      901 gcttcatcac cctggcggag gtaacagata aggcggaaaa ctgcaggaga gcaatcgaag
      961 catgctctgc ttccctggag cattaccaaa aagatgaggc accaagagag catgctgaga
     1021 tcttaagaga cctggccttc gcttatgtca ccatatctga ggtggaggac agagaggaat
     1081 gggtcaaaag ggctctgcgg gcttataaga aggccaccag gatattcgag gggcttgccc
     1141 aggagcttga ggcagaaaga gacccggaag ctgcagaaat gcgcaacgat gtccagagat
     1201 gccgccgctc catgcaatca tgcaagggca tactcaaggc cagcaggaag aggagatcag
     1261 aggttcagcc actggaaagc aggtagcatg aaggatttag cagagagcga taagatggag
     1321 tacttccgcc gctcctaccg gtcagtggac ggtctttggt tcatgatgat cgagaagaaa
     1381 cggggatttg atgaagcgct ggagctggac gaggctgtct ggcgggttct gcccaagatc
     1441 caggcccggg caatcaagtc aatgctgatg ctggactatg gccttgatgg gctaaaggag
     1501 gcgattgctg ctcgtctggt cctggaggag ttcgactacg agctgaaaga agaggaggac
     1561 ggatttgcag ttgagatccg gcgctgtccc tggcatgata taatggtccg gtcaggcaga
     1621 gaatcgctct ccgagaaggt gagcgaggtc atctgccagg cggagaactc tgtctgggcg
     1681 caggagttca gt
//
LOCUS       DAOSGP010000298         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_73101_length_1690_cov_18.389602, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000298 DAOSGP010000000
VERSION     DAOSGP010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73101_length_1690_cov_18.389602"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /gene="phrB"
                     /locus_tag="PLV49_07115"
     CDS             <1..1196
                     /gene="phrB"
                     /locus_tag="PLV49_07115"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOG98647.1"
                     /translation="SPLAVLFCISPQFLEATSSQYKFMLKGLKEVEADLAELNIPFYL
                     LSGDPAKEIPQFLQSHEAGILVSDFSPLRIKMIWSRSIAKEVNIPCYEVDGHNIVPCW
                     QASLKQEWAAYSFRPKIHRLLDEYLTDFPALKAHPFPWPEAVDNDWRGAEMAIRADHF
                     SEMSWAQPGERAAKKHLDEFIDRGLIRYDSDRNNPVKNGQSGLSYYLHFGQISAQKVA
                     LMALSGMTDSSPFLEELVVRRELADNFCYYNPHYDSVQGFPNWARETLRQHENDPREY
                     LYSIKELEEAKTHDELWNAAQTEMVCRGKMHGYMRMYWAKKILEWTSSPAEAMKIAIY
                     LNDRYELDGRDPNGYTGIAWSIGGVHDRAWKERPIFGKVRYMSYNGAKTKFDIKAYIE
                     KINSF"
     gene            complement(1188..1679)
                     /locus_tag="PLV49_07120"
     CDS             complement(1188..1679)
                     /locus_tag="PLV49_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG98648.1"
                     /translation="MIFKMPSMKKQEYDRLIEEEFICRITFNGESHPHVAPFLYVFDG
                     KFMYFLSTKYGKKVLHFRKNPAVTVEVERYSPDLSSFSFVALPGRLVEVEDTETKSLV
                     RERFVELIQKKNLSSNVLSALGHSPDEPVEALLTEGRNSVWKLVGVKMGNISGLKHSD
                     GSK"
BASE COUNT          420 a          427 c          451 g          392 t
ORIGIN      
        1 agtctcccct ggcggtgctc ttctgcatct cgccccagtt cctggaggca acaagctctc
       61 aatataagtt catgctcaaa gggctaaaag aggtagaggc tgatcttgct gaattgaata
      121 tccccttcta cctgctctct ggagatccgg caaaagagat cccgcagttt ctccaatctc
      181 atgaagcagg aatcctggtc tcggacttct ctcctctgcg aataaagatg atctggtcaa
      241 gatcaatagc taaagaggtc aatattccat gctatgaggt tgatggccac aacatcgttc
      301 cctgctggca ggcttcttta aagcaggagt gggcggcata cagcttccgt ccgaagatcc
      361 accgtctcct ggatgagtac ctgacggact ttcctgcgct gaaggctcac cccttcccct
      421 ggcctgaggc agtggacaac gattggaggg gagcagagat ggcgatcaga gcggatcatt
      481 tttccgaaat gagttgggca cagcctgggg agagggcggc gaagaagcat ctggatgagt
      541 tcatagatcg ggggcttatt cgctacgact ctgatcggaa caatccagtg aaaaacggcc
      601 agtccggcct ctcatactac cttcatttcg gccagatatc cgctcagaag gtggccctga
      661 tggcgctgtc tggcatgacc gattccagcc cattcctgga ggagctggtg gtgcgacgcg
      721 agctggcaga taacttctgc tactacaatc ctcactacga ctctgtgcag ggctttccca
      781 actgggcgcg ggagaccttg aggcagcatg agaacgatcc gcgcgaatac ctgtatagca
      841 tcaaggagct ggaggaagca aagacccatg acgaattatg gaatgcggcc cagacggaga
      901 tggtctgccg ggggaagatg cacggataca tgaggatgta ctgggcgaag aagatcttgg
      961 agtggacttc ttctcctgca gaggccatga agattgccat ataccttaac gatagatacg
     1021 agctggacgg aagggacccc aacggatata cagggatcgc ctggtctatt gggggagtgc
     1081 acgatcgtgc ctggaaggag aggccgatct tcggcaaggt gcggtacatg agctacaacg
     1141 gagcaaaaac caagtttgat atcaaggcct acattgagaa gatcaattca ttttgatcca
     1201 tccgagtgct tcaaaccaga gatatttccc attttaacac caaccagctt ccagacgctg
     1261 ttgcgaccct cggtgagcaa tgcctctaca ggttcatctg gagagtgccc cagggctgat
     1321 agcacattgg aggagaggtt tttcttctgg atcagctcca caaacctctc tctcacaagg
     1381 gacttggttt cagtgtcctc tacctccacc agccttccgg gcagggcgac gaatgaaaag
     1441 ctggagagat ccgggctgta tctctcaacc tccactgtaa ccgcgggatt cttcctgaag
     1501 tgaaggactt tcttgccata ttttgtggac aggaaataca tgaacttgcc atcgaatacg
     1561 tacaggaacg gggctacatg gggatggctc tccccattaa aggttatgcg acagatgaac
     1621 tcctcttcta tcagccggtc gtactcctgc tttttcatcg agggcatttt aaaaatcata
     1681 aagagccttt
//
LOCUS       DAOSGP010000299         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_73227_length_1688_cov_19.995713, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000299 DAOSGP010000000
VERSION     DAOSGP010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73227_length_1688_cov_19.995713"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLV49_07125"
     CDS             <1..429
                     /locus_tag="PLV49_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOG98649.1"
                     /translation="CALIAVCGDFNEEFDEVSIEAIRGDVENTGNMDLAMRVMVPTER
                     NIPEPARYSLLHNGKGKMLDHILVSRTMLAFFKGSEVHNELLHDESIVFAPKIFYPES
                     DHAPVIARFELPESDIRWSTTTSVLRRERGRDKSHRSRYK"
     gene            445..963
                     /locus_tag="PLV49_07130"
     CDS             445..963
                     /locus_tag="PLV49_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG98650.1"
                     /translation="MSKKSGGILPFRFQDNHLEVLLVHPGGPLWAGKDDGAWSISKGL
                     FEENESPLDAARREFKEETGFEVDGVFIELGRIKQPSGKIVFAWAVEKNLDERRIVSN
                     KFMLEWPRRSGIMREFPEIDRADWFEIESARKKILKGQQGFIDRLLHALDHPNVHHPE
                     IDPDEFISIERS"
     gene            complement(941..1645)
                     /locus_tag="PLV49_07135"
     CDS             complement(941..1645)
                     /locus_tag="PLV49_07135"
                     /EC_number="4.2.3.153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HOG98651.1"
                     /translation="MRLLVSPMNMEEAHAALAGGADILDVKNPKEGSLGANFPWVIRS
                     VADLAGGRVPVSATIGDMEFKPGTASLAALGAASSGADYVKAGLLGVKTCDQAEEMLK
                     AIVRAVKDLDPKKKVVASGYSDYLRVGSISPMLLPAAASEAGADVVMVDTAIKDGKPT
                     FDFMGEKDLADFIELGHSFDLEVALAGSIGFPHLGTLLRLQPDIIGVRGIVCGGDRRS
                     AVKEELVVKVKSALSR"
BASE COUNT          391 a          459 c          463 g          375 t
ORIGIN      
        1 tgtgctctca ttgccgtctg cggggatttc aacgaggagt tcgatgaggt ctccatagag
       61 gctatccggg gtgacgtgga gaacacgggg aacatggacc tggccatgag ggtcatggtg
      121 ccaacagaga gaaacattcc cgagcccgca cgctattctc ttctgcataa cggcaagggc
      181 aagatgctgg accatattct tgtatcaagg acgatgctcg ccttcttcaa gggctccgag
      241 gtgcacaacg agctcttgca tgatgaatcc atagtctttg ctcccaagat cttctatccc
      301 gagtccgatc atgccccggt gatcgcccgc tttgagctgc cggaatcgga tattagatgg
      361 tccaccacga caagcgtctt gagaagggag agaggcagag acaagagcca tcgatcaaga
      421 tacaagtaag ttgataaaaa taatatgagc aaaaagagcg gcgggatcct gcctttcagg
      481 tttcaggaca atcatcttga ggtcttgctc gtccaccccg gtgggccctt gtgggccggg
      541 aaggacgatg gagcctggtc gatatccaaa ggccttttcg aggagaacga gagccccctg
      601 gatgcagcaa gaagggagtt taaggaagag acgggctttg aggttgatgg ggttttcata
      661 gagctgggaa ggataaagca gcccagcgga aagatcgttt ttgcctgggc ggtggaaaag
      721 aacctggatg agaggaggat tgtgagcaat aagttcatgc ttgagtggcc caggagatcg
      781 gggataatgc gggagtttcc cgagatcgac agagcagact ggtttgaaat agagtctgcc
      841 cggaaaaaga tcctcaaagg acagcagggt ttcatcgacc ggctgctgca tgccctggat
      901 catccaaatg tgcatcatcc cgaaattgat ccggatgagt tcatctcgat agagcgctct
      961 tgaccttcac caccagttct tctttcaccg cactcctgcg gtctcccccg cagacgatgc
     1021 cccgcacgcc gatgatgtcc ggctgcaggc gcaagagcgt tcccagatga ggaaatccga
     1081 tgctgccagc cagggcaacc tccaggtcaa atgagtggcc aagctcaatg aaatccgcca
     1141 agtccttctc gcccatgaag tcgaatgtcg gcttcccatc ctttatggcg gtgtccacca
     1201 tcaccacatc tgcccctgcc tcagaggcgg ctgcgggcag gagcatggga gatatgcttc
     1261 caacacgaag gtagtcagag tagcctgaag ccaccacctt cttctttgga tcgaggtctt
     1321 ttaccgctcg cacgatggct ttgagcatct cctctgcctg atcacatgtt ttcactccca
     1381 ataggcccgc tttgacgtaa tccgcgcctg aggacgctgc gcccaaagct gccaggctcg
     1441 ctgttcccgg cttgaactcc atgtcgccga tggtcgcact taccggcacc ctgcctcctg
     1501 ccaggtctgc taccgagcgt atgacccagg ggaagttcgc acccagggat ccctctttgg
     1561 ggttcttcac atccaaaata tcagcgccgc ccgccagtgc agcatgcgcc tcttccatgt
     1621 tcatggggct gaccaataat ctcatattct ctcaatcccg attgggcaaa tgtgcaatgc
     1681 atctttgt
//
LOCUS       DAOSGP010000300         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_73352_length_1686_cov_19.122011, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000300 DAOSGP010000000
VERSION     DAOSGP010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73352_length_1686_cov_19.122011"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLV49_07140"
     CDS             complement(<1..248)
                     /locus_tag="PLV49_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HOG98652.1"
                     /translation="MDYSFKRGFKPDMERIRKVLEEEFPAEIKESDGMLSLSFGALKS
                     IQVSINNKKLNVTTESYSDAKDDQILDTNKRFRNFLEKA"
     gene            complement(378..1628)
                     /gene="eno"
                     /locus_tag="PLV49_07145"
     CDS             complement(378..1628)
                     /gene="eno"
                     /locus_tag="PLV49_07145"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOG98653.1"
                     /translation="MSFEIEKIHAREILDSRGNPTVEVDVYTCCGFGRAAVPSGASTG
                     THEALELRDGGDRYDGKGVLKAVKNVNEVIAPELLGMDASCQREIDDLMINMDGTANK
                     ANLGANSILGVSIAMAKAAAMSSGVPLYKYLGGVNTVTLPIPSFNVLNGGKHAGNELS
                     IQEFMIQPTGAKTFSEGLRMAAETYHALGKILQGKYGVGATNVGYEGGYAPPLIKTRD
                     ALDAIMSAFDVSGYGDEIKIGLDAAASSFYMEGGYSVDGNRLSPGELIDYYVDLVNTY
                     PIILLEDPFEEEAYDDFARLTAKLPGTIIVGDDLYVTNIKRLEKGIKMCAGNALLLKL
                     NQIGTVSEAFDAARMSFRSGWKVMASHRSAETEDSALADVAVALGCELLKTGAPARSE
                     RTAKYNQLLRIQEDLGEAARYACI"
BASE COUNT          359 a          469 c          434 g          424 t
ORIGIN      
        1 gctttttcca aaaaatttcg gaagcgcttg ttggtgtcca ggatctgatc gtcttttgcg
       61 tccgaatacg attcagtggt gacattgagc ttcttattat tgatcgatac ctggatgctt
      121 ttcagagcgc caaaggagag cgagagcatt ccgtccgact cttttatctc tgcggggaac
      181 tcctcttcca gaacctttct gattctctcc atgtcgggct tgaatccgcg cttgaatgag
      241 taatccatgt gagaaggatg aattctgatc gagtaataat gtttcgagcc gttttgccca
      301 ttacccctta aaaaagagcg ctcatcctcc catctcagga ggaaaagcgg gtgatgttct
      361 cactgatgca tccatattta gatgcaagcg tatcttgctg cctcgcccag atcttcctgg
      421 attctgagga gctggttgta cttggccgtc ctctcggaac gggcgggagc gccagtcttc
      481 agcagctcgc agcctaaggc cactgccaca tctgccagag cggagtcctc ggtctctgca
      541 gagcgatggg atgccatgac cttccatcca gagcggaagg acatccgagc agcgtcaaag
      601 gcctcggata ccgttccgat ctggttaagc ttgagaagca gtgcattgcc cgcgcacatc
      661 ttgattccct tctccaggcg cttgatgttg gtgacgtaca ggtcgtctcc cactatgatc
      721 gttcccggaa gcttggcagt gaggcgggcg aagtcatcat aggcctcctc ctcgaagggg
      781 tcctccagca aaatgatggg ataggtattg accagatcca cataataatc tatgagctcg
      841 ccaggtgaga gcctgtttcc gtccacagag tagccgccct ccatatagaa gcttgaggca
      901 gcagcatcaa ggccgatctt gatctcatct ccgtaacctg atacgtcaaa ggcgctcatg
      961 atggcgtcca gggcatcccg ggtcttgatg agaggtggtg catagccccc ctcgtatcct
     1021 acgttcgtcg cccccacgcc gtatttccct tgcaggatct ttcccagagc atggtaggtc
     1081 tcggcggcca tgcgcaggcc ctcgctgaag gttttggccc cggttggctg gatcatgaac
     1141 tcctggatgg atagctcgtt tccggcatgc ttgcccccgt taaggacgtt gaaagagggg
     1201 atgggaagag ttacggtatt cactccgccc aggtatttgt agagcggcac cccagaggac
     1261 attgccgccg cctttgccat agctattgag actcccaaga tggaattggc gcccagattg
     1321 gccttgttcg cggttccgtc catatttatc atgagatcat cgatctccct ttggcaggag
     1381 gcatccatgc ctaaaagctc aggagcgatc acctcattca cattcttgac cgccttcagg
     1441 acgccttttc cgtcgtacct gtcgccgcca tccctgagct ccagggcctc gtgagtgcct
     1501 gtagatgcgc ccgaaggcac agccgcccgt ccaaagccac agcaggtgta gacatcgacc
     1561 tcgacagtcg gattgcctcg tgaatcaagt atctcacgag catgaatctt ctcgatctcg
     1621 aatgacatcg tataccacaa ccggtagttt gcagatctat ttattaaagt tagcgtgccc
     1681 tgaatc
//
LOCUS       DAOSGP010000301         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_74276_length_1673_cov_13.698393, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000301 DAOSGP010000000
VERSION     DAOSGP010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74276_length_1673_cov_13.698393"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLV49_07150"
     CDS             complement(<1..296)
                     /locus_tag="PLV49_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOG98654.1"
                     /translation="MNLAGPTVLAFGGNALLLDPRNPATQERTAVEFARAVRLLMDQG
                     EGMVLVHGNGPQVGMILLRIEATKDCIPPETLDIMVAETQGSIGYLLCRSMRNE"
     gene            complement(293..1669)
                     /locus_tag="PLV49_07155"
     CDS             complement(293..1669)
                     /locus_tag="PLV49_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOG98655.1"
                     /translation="MYTADKKRERILILGAAGRDFHNFNVFFRSRPQFEVVGFTAAQI
                     PDIACRLYPPELSGGLYPAGLPIWPEDALEEIIIKCRVDRCILSYSDLSHEDVMHLAE
                     RVLATGTDFGFLGGRNTMLKSKKLVIAVCAVRTGAGKSQVVRYIADIIRQAGLKAVVV
                     RHPMPYGDLARQEVQKFSSRADLNAADLTIEEREEYERHIDKGTVVYAGVDYQTILDQ
                     AEREADVILWDGGNNDTPFFKPDLWITVADALRPGHEISYYPGETNFLAADLILINKA
                     NSAAEEDIITIQANASRLNPKAGVVVAGSEVTAEDPKAISGKRVLVVEDGPTITHGEM
                     AYGAGQVAARRYNAAEIIDPRPYAVGSLKEIFSKFRHIGRVLPAMGYYPQQIRELEET
                     INKAECDSVIIASPIDLRRLIQINKPSTYVLYDLVDMDRPYLREKIEEFISKSIPKSM
                     RDSASKGD"
BASE COUNT          351 a          486 c          413 g          423 t
ORIGIN      
        1 atctcgtttc tcatagatcg gcaaagaagg tagccaatgc tcccctgcgt ctcggcaacc
       61 atgatatcaa gagtctcggg cggtatgcag tctttcgtgg cctcaatccg cagcagtatc
      121 atgcccacct gagggccgtt gccatgaaca agcaccattc cctctccctg gtccataagc
      181 agcctgaccg cccgggcaaa ttctactgct gttctctctt gggttgcagg attgcgagga
      241 tcgaggagaa gggcatttcc gccaaaggct aaaaccgtcg gtcctgccag attcaatccc
      301 ccttggatgc ggagtctcgc attgatttgg gtatggattt ggatatgaac tcctcgatct
      361 tctcccttaa atatggccga tccatgtcca ccaggtcgta aagaacatag gtggatggtt
      421 tattgatctg gatgagccgg cgcaggtcta tgggcgaggc gatgatcacc gaatcgcatt
      481 cagctttatt tatagtctct tccaattccc tgatctgctg ggggtagtag cccatggccg
      541 gaaggaccct tccgatatgt ctaaacttcg agaagatctc ctttaaggat ccgacggcat
      601 aaggccgggg atcgatgatc tctgctgcat tgtacctcct ggccgccacc tggccggcac
      661 cataggccat ctccccgtgg gttatggtcg gaccgtcctc aactaccagc accctcttgc
      721 cagatattgc tttgggatct tctgccgtga cttctgatcc ggccaccact actcctgctt
      781 ttggattcag ccttgatgca ttggcctgta tagttatgat gtcctcctct gcagcggagt
      841 tggctttgtt gatcaggatg agatctgcag ccaggaaatt cgtctccccg gggtaatagg
      901 atatctcatg ccctggtcgg agagcatcgg caacggtaat ccacagatca ggcttgaaaa
      961 acggggtgtc gttgtttccc ccatcccaga gaataacatc cgcttccctc tccgcctgat
     1021 caagaatagt ctggtagtcc actcctgcat agactactgt gcccttgtct atatgcctct
     1081 catactcctc cctctcctcg atggtcagat cagcggcgtt cagatccgct cgagaggaga
     1141 acttctgcac ctcctgtcga gccaaatctc catagggcat gggatgcctc accacaaccg
     1201 ccttcagccc ggcctggcga atgatgtctg caatgtatct aacaacctgg cttttgccgg
     1261 ctccggttct cactgcgcag accgctatca ccagcttctt tgacttgagc atagtattcc
     1321 gtccgcccag gaatccgaag tctgtgcccg tggccagtac cctctctgcc aggtgcatca
     1381 catcctcatg gctcaggtcg ctgtaggata gaatgcatct gtccacccgg catttgatga
     1441 ttatctcctc aagggcatcc tcaggccaga tgggaaggcc agccggatag agcccaccgc
     1501 tgagctctgg cggataaagg cggcaggcaa tatccggtat ctgagcggca gtgaatccca
     1561 ccacctcgaa ctggggcctg ctgcgaaaga atacattgaa gttatggaag tctctgcctg
     1621 ccgccccgag gattaaaatt cgctccctct tcttatctgc tgtatacatg atc
//
LOCUS       DAOSGP010000302         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_74599_length_1668_cov_16.085555, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000302 DAOSGP010000000
VERSION     DAOSGP010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74599_length_1668_cov_16.085555"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLV49_07160"
     CDS             <1..810
                     /locus_tag="PLV49_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98656.1"
                     /translation="ELFALDKSWSEQMVAYVLALLQGNLGFSYYYKDEVSSVIMGALP
                     WTLLLVSLSLLIATVVGVMLGIESGYRRGERIDRGLLAGLMLLGGLPDFFLGIVLLLI
                     FGVSLNLFPLSGALTPYSGLEGLPLLADIARHLALPLAALVLVDLGGIYLLTRNTVVT
                     ILKEGFMLTARAKGCSEFCLRYSHAGRNALLPVTTATGLRIPQLITEALFIEIVFSYP
                     GLGLMLRTALDARDYPLIQGILLLVTITVLFANMFVDHIYKRLDPRIRYAH"
     gene            800..>1668
                     /locus_tag="PLV49_07165"
     CDS             800..>1668
                     /locus_tag="PLV49_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG98657.1"
                     /translation="MHIDLGHEIRHDRMALLGILVLAVILLMAIFAPLLSPYSPAEYT
                     GKIFSSPSNDHLLGTDSMGQDIWTRLLFGARTSLLVAASVALLASSLSVLIGASSALV
                     GGLYERFWMRIVDVMICLPTIIVMILIAAYFRPNLPLLILLISAFTWPGGARIVRSQV
                     LTLKERMHIYAARTFGADQRHILKTHIVPDISPIIASMMIQDARRAVFMEAGLAFLGV
                     SDPLIISWGKMMNQALAFTYLDVWKWWLLPTGILLSLTLVSLSFLGVSLEAAMDPRLC
                     RHEHEHGHDHEHE"
BASE COUNT          316 a          502 c          434 g          416 t
ORIGIN      
        1 gagctcttcg ccctggacaa atcctggtcg gagcagatgg tcgcctatgt cctcgctctc
       61 ctgcagggca acctcggctt ctcttactac tataaggatg aggtctcctc agtcatcatg
      121 ggtgctttgc cttggactct gctcctcgta agcctgtcct tgctgatagc caccgtggtg
      181 ggcgtgatgc tgggaatcga gtccggatac cgccggggtg agagaataga caggggactg
      241 ttagccgggc tcatgctcct gggcggcctt cccgacttct ttttaggaat tgtcctcctg
      301 ctcatattcg gggttagcct gaacctcttt cccctgtccg gcgctctcac cccctactcc
      361 ggcctggaag gcttacctct gctcgcagat atcgcaagac atctcgccct gcccctggcc
      421 gctctggttt tagtggatct gggcggcatc tatcttctca ccagaaacac cgttgtcacc
      481 atattaaagg agggattcat gctcaccgcc cgggccaagg gctgctctga attctgcctg
      541 cgctattcac atgcaggaag aaacgccctc ttgcccgtga ccaccgccac cggcctgcgc
      601 attcctcagc tgatcacaga agcccttttc attgagatcg tcttttccta tcccggcctc
      661 ggactgatgc tccgaactgc tttggatgcc cgggactatc ccctgatcca gggaatactt
      721 ctcttggtga cgatcaccgt tcttttcgcc aatatgtttg tcgaccatat ctacaagagg
      781 ctggacccga ggatcagata tgcacattga cctcggccat gagatcaggc acgataggat
      841 ggcgctcctg ggcatcctgg tgctggcagt tatcttgctc atggccatat tcgcgcccct
      901 tctctccccc tactctcccg ccgaatacac cggcaaaata ttcagttccc cctcgaatga
      961 tcatcttctg ggcacagact ccatgggcca ggacatctgg accagattgc tcttcggggc
     1021 gagaacctcc ctcttggtgg cagcatctgt ggccctgctc gcctcttccc ttagcgtctt
     1081 gatcggagcc tcctccgcgc tggtgggcgg gctctatgag cgcttctgga tgagaatagt
     1141 ggatgtgatg atctgccttc ccacaatcat tgtgatgatt ctgattgcgg cctacttcag
     1201 gccaaacctg ccactattga tcttgctgat atccgccttc acctggccag gaggagcgag
     1261 aatcgtgcgc tcccaggtcc tcaccctgaa ggagcggatg catatctatg ccgcaaggac
     1321 ctttggagca gaccagaggc atatactcaa gacccatata gtgcccgata tctctcccat
     1381 catcgcctcc atgatgatcc aggatgccag gagggcggta ttcatggagg cggggctggc
     1441 atttcttgga gtctcagatc cgctgatcat cagctggggg aagatgatga atcaggcatt
     1501 ggccttcacc tatcttgatg tatggaagtg gtggctgctt cccaccggca tccttctgtc
     1561 gctcaccctg gtgagcctga gctttttagg agtgagcctg gaggcagcca tggaccccag
     1621 gctgtgcaga catgagcacg agcacggaca cgatcatgag catgagca
//
LOCUS       DAOSGP010000303         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_75334_length_1658_cov_18.031192, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000303 DAOSGP010000000
VERSION     DAOSGP010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75334_length_1658_cov_18.031192"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLV49_07170"
     CDS             <1..396
                     /locus_tag="PLV49_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98658.1"
                     /translation="DMAETQKQIDTYNRNIDRAPEVLKSILGDEKINLEVLRKNASTF
                     QVGLDVKSARIDRLIEGGWDDPSITITASEEAIDNVRLSKDPIGAFEKERDAGSITFE
                     ANNLISRAKLKAAFSSTSVLQFGYDTFFG"
     gene            complement(393..>1658)
                     /gene="thiC"
                     /locus_tag="PLV49_07175"
     CDS             complement(393..>1658)
                     /gene="thiC"
                     /locus_tag="PLV49_07175"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOG98659.1"
                     /translation="ARAGRLTEEMKIVAEAEGKTPEFILRGIASGRIVIPRSPYRYTR
                     SVGIGKGLRTKVNASIGTSSDIVDVDMEVAKARTAEANGADTLMELSTGGDFLEIRRR
                     VIEATTLSVGSVPLYQAFIEAIRKYGAGVDMPEDDLFRATEEQAKLGTNFMAIHTGIN
                     SICIERLRRQGGRFGGVCSRGGAFMIGWMLHNQKENPLYREFDYLLEILKEHEVTLSL
                     GNGLRAGAVHDSTDRAQIQELIINSELADRAHAAGVQTIVEGPGHIPIDEIEANVLVM
                     KRMTNEKPFYMLGPLVTDIAPGYDHIVAAIGASLSSAYGADFICYVTPAEHLALPDVE
                     DVRMGTITARIAAHIGDMIKNNDRQEDLDMGRARRDMLWEKQFQLAIDPETAKLIRAD
                     RTPSNAEVCTMCGDFCALKIIKENIDLAR"
BASE COUNT          357 a          478 c          427 g          396 t
ORIGIN      
        1 gacatggcgg agacgcaaaa gcaaatcgat acgtacaacc ggaatatcga tcgagcgcca
       61 gaggttctga aatccatttt gggcgatgag aagatcaacc tggaagtact ccgaaaaaat
      121 gccagcacct ttcaggtggg cctggatgtg aaatcagcga gaatagacag attaattgaa
      181 ggaggctggg acgatccatc aatcaccata actgcctcag aggaggcaat agacaatgtc
      241 agattgtcaa aggatccgat cggagccttt gaaaaagaga gggatgcagg cagtatcacc
      301 ttcgaggcaa ataatctgat ctccagggcc aaattgaagg ccgctttctc cagcacctct
      361 gtcctgcagt tcggttacga cacattcttc ggctagcggg cgaggtcaat attctctttt
      421 attattttta aggcacagaa atccccgcac atggtgcaga cctcagcatt cgagggggtt
      481 cggtctgctc gaatcagctt tgcagtctcc ggatctatgg ccagctggaa ctgcttctcc
      541 cagagcatat ccctccgcgc ccttcccata tccagatcct cctggcgatc gttgttcttg
      601 atcatatcgc ctatatgggc tgctatcctg gctgtgatcg tgcccatcct cacatcctcc
      661 acatcaggaa gagccagatg ctcagccggg gtgacatagc agatgaagtc cgctccgtaa
      721 gcactagaga ggctggcacc aattgccgcc acgatatggt cgtatccggg agcgatatct
      781 gtgaccaggg ggccgagcat atagaagggc ttctcattgg tcatcctctt cataaccaga
      841 acatttgctt cgatctcatc gatggggatg tggcccggcc cctccactat ggtctgaacc
      901 cctgccgcat gggctctgtc cgccagctcg gagttgatta tcagttcctg gatctgagcc
      961 ctatcggtag agtcgtgcac cgctcctgct cgaaggccgt tgcccaggct gagggtgacc
     1021 tcatgctctt tgaggatctc caagagataa tcgaactccc ggtagagggg attctccttc
     1081 tggttatgca gcatccagcc gatcatgaag gcccctcccc ggctgcatac gccaccaaat
     1141 cgacctccct gacggcggag ccgctctatg cagatgctat tgatgccggt gtggatggcc
     1201 atgaagttgg ttcccagctt cgcctgctcc tccgtcgctc ggaagaggtc gtcctcgggc
     1261 atatccactc ctgcgccata ctttcttatg gcctcgatga atgcctggta gaggggaacg
     1321 ctccccacgc tcaatgtggt ggcctcaatc acacgtcttc tgatctccag gaaatcccca
     1381 cccgtcgaga gctccatcag agtgtctgca ccgttcgctt cagcggtgcg cgcttttgcc
     1441 acctccatgt ccacatcaac gatatcgctg ctggtgccga tggaggcgtt caccttggtg
     1501 cggagtccct tgccaatgcc aaccgatcta gtgtatctgt aagggctgcg agggatgact
     1561 atcctgccgc tggctatgcc tcgaagtatg aactcagggg tctttccctc tgcctcggcc
     1621 actatcttca tctcttccgt cagtctgcct gctcgtgc
//
LOCUS       DAOSGP010000304         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_75555_length_1655_cov_18.208750, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000304 DAOSGP010000000
VERSION     DAOSGP010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75555_length_1655_cov_18.208750"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1195
                     /locus_tag="PLV49_07180"
     CDS             104..1195
                     /locus_tag="PLV49_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOG98660.1"
                     /translation="MAAKETIAEPTYLECGFGELEYKVIKSDVCCRCGTCEALCPRIE
                     HVEDKPELVMYDPHCGLCFTYCPRNILDMGQMESKLLGRTRNPEEKLGIYKKAISAQA
                     VSREGLAQDGGVATALLVFALESGMIDCAIVTDKDDEWRTVSRVVTTADEIRAAAGTK
                     YTINNSVNSLAEALNMGFEKIGFVGTPCQIQGLRKVQLLEEPYQIGQEKIALLVGLFC
                     MENFDYDLLMNGLVKGRFGLDPKNVARFEIKKGMFRVIDKQGKVNEVKLEETDQYNFK
                     GCGPCFDFASELADISVGSVGSGDGWSTVLTRTDKGEKLYSDALAAGVLQEKAVSEKG
                     LALAGKLAENKEKRFKAKSAEMNVSGISR"
     gene            1216..>1655
                     /locus_tag="PLV49_07185"
     CDS             1216..>1655
                     /locus_tag="PLV49_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOG98661.1"
                     /translation="MLSVGLAGKPNSGKSTFFKAATLVEVDIANYPFTTIDANHGVSY
                     VRVPCPCKDLGIEKGCGRCKDGVRFIAIELIDVAGLVPDAHLGKGLGNEFLDALRVAE
                     AVIHVLDASGGTDAEGNPVGTGNYDPVSDVKFLEYEISMWLNGIL"
BASE COUNT          383 a          408 c          509 g          355 t
ORIGIN      
        1 ccaatatcca gacccaggat catctacgct caaacctcat tcgaaccata aaagctttag
       61 gtcgggaggt gatttagccc tctaactttt ggagatcatg atcatggctg ctaaggagac
      121 gattgcagag cccacttacc tggaatgtgg tttcggagag ctggagtata aggtcataaa
      181 atcggatgta tgctgtcgct gtggcacatg cgaggccctc tgccccagga ttgagcatgt
      241 ggaggacaag ccagagctgg tgatgtacga tccccactgc ggcctgtgct tcacctactg
      301 tcccagaaac atcctggata tgggtcagat ggagtccaag ctcttgggca ggactcgtaa
      361 tcccgaggaa aagctgggca tatacaagaa ggccatatcc gctcaggctg tctcccgcga
      421 gggtttagcc caggatggcg gcgtggccac tgctctgctt gtttttgcgc tggagtcggg
      481 gatgatcgat tgcgccatcg tcaccgacaa ggatgatgag tggaggacgg tatcacgggt
      541 tgttaccacc gccgatgaga tcagggctgc cgctggcacc aaatacacca taaacaatag
      601 cgtcaattca ttggctgaag ccctaaacat gggattcgaa aagatcggat ttgtaggaac
      661 tccctgccag atccagggcc tcagaaaggt tcagctgctg gaagagccct atcagatcgg
      721 ccaggagaag atcgctctgc tcgtcggcct gttctgtatg gagaacttcg actacgacct
      781 gctcatgaac ggcttggtga agggcaggtt tggcctggat cccaagaatg tggcccggtt
      841 tgagatcaag aagggtatgt tcagggtgat agataaacag ggcaaggtca atgaggtgaa
      901 gctggaggag actgaccagt ataacttcaa gggatgcggt ccatgcttcg actttgccag
      961 cgagctggcg gacatctccg tgggcagcgt gggatcgggt gatggctggt cgactgttct
     1021 cacccgcacg gacaaggggg agaagctcta ctctgatgcc cttgcggcag gtgtcctgca
     1081 agagaaggct gtctcggaga agggcctggc tctggccggc aagctggcgg agaacaagga
     1141 gaagcggttt aaggccaagt cagcagagat gaacgtttcc ggcatctccc ggtgagccag
     1201 gacagttgaa ctgatatgct ctctgtcggg ctggctggaa agccgaactc tggtaaatcc
     1261 acctttttca aggccgcgac cctggtagag gtggatatag ccaactatcc ctttaccacc
     1321 atcgacgcca accatggcgt ctcttatgtg cgcgtcccat gcccctgcaa ggatctggga
     1381 atcgagaagg gctgcggaag atgcaaggac ggggtgcggt tcatcgccat cgagctgatc
     1441 gacgttgccg gcctggtgcc ggacgctcac ctgggcaagg gcctggggaa cgaattcttg
     1501 gatgccctgc gagtggcgga ggcggtgatt catgtgctcg atgcttcggg cggcacagat
     1561 gccgaaggaa acccggtagg tacgggcaac tacgatccgg tctccgatgt aaaattttta
     1621 gagtacgaga tcagcatgtg gcttaacggc atcct
//
LOCUS       DAOSGP010000305         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_76195_length_1646_cov_19.230044, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000305 DAOSGP010000000
VERSION     DAOSGP010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76195_length_1646_cov_19.230044"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLV49_07190"
     CDS             complement(<1..698)
                     /locus_tag="PLV49_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98662.1"
                     /translation="MIPALLLLLMLLLPGCSALDVQVGPMAQPAGAVILVIDGLGSSY
                     IYPEHNAYALDGSPLDKAVLFNLTGGGARAVDVRVPVPETTKSHSVLVTGRSDADWDQ
                     LGPTVFDLAREEGYLCLAIMERGDSMSILEDMDAVLYLEDNALQGAEPTPGFRPDAPE
                     GLRTLFQEWRDRFAGYSNREGMAGYAGYNAWALDAAADTVEHLIGKPFIMLVNAGAVD
                     SAGHNLNASGYLQAV"
     gene            738..977
                     /locus_tag="PLV49_07195"
     CDS             738..977
                     /locus_tag="PLV49_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98663.1"
                     /translation="MYCFKGKLYLLTILTTLSMLATKRIPVTEAVWIELSELKSAGQT
                     YSQLLEEMIEDRKKARLFRDMEKIEKEGEFVELPW"
     gene            989..1261
                     /locus_tag="PLV49_07200"
     CDS             989..1261
                     /locus_tag="PLV49_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG98664.1"
                     /translation="MINKDALEFINSLPSKSHRIVVEKCKTLAINPFPGQGDKEFIQR
                     KDHEDIYRLHISRSYTAFYKIHKNEKIVRILDIMTIEQAHKIYGRF"
     gene            complement(1368..>1646)
                     /locus_tag="PLV49_07205"
     CDS             complement(1368..>1646)
                     /locus_tag="PLV49_07205"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOG98665.1"
                     /translation="ELHRAIDMEKSAHAWQNIPDIKALAEEGRRLLVAVAGGIRTDTQ
                     GAALAAGADILVVGRAITRSRDIRDMAEQFLTGLKQTEIDQYRIMTDF"
BASE COUNT          410 a          436 c          414 g          386 t
ORIGIN      
        1 actgcttgca gatagccgct ggcgttcaga ttatggccgg cgctgtccac cgctccggca
       61 ttgaccagca tgatgaatgg ctttcctatg agatgctcta cagtatccgc cgccgcatcc
      121 agtgcccagg cgttgtagcc ggcatagcct gccatgccct cacgattgga atatccagcg
      181 aatctgtccc gccattcctg aaagagggtg cgcaggccct ctggagcatc gggccgaaat
      241 cccggtgtgg gctcagcgcc ctgcagggca ttatcctcca agtacaggac tgcatccata
      301 tcctccagga tcgacatcga gtctccccgc tccataatgg caaggcataa atatccctcc
      361 tcccgggcga ggtcgaagac agttggtccg agctggtccc agtccgcgtc cgatctgccg
      421 gtgaccagga ccgaatggct ctttgtggtc tcggggactg gcactcgcac atccaccgcc
      481 cgtgccccac cgccggtgag gttgaagagc accgccttat ccagaggcga gccgtccagg
      541 gcataggcat tgtgctccgg atagatgtaa gatgatccca ggccgtctat gaccagtatg
      601 actgcgccag cgggctgagc catgggcccc acctggacgt ccagagcgct gcagccgggc
      661 agcaatagca tcaagaggag gagaagggca gggatcatcc ctccgggctt agtcagccaa
      721 atattaaaat ctttcatatg tattgcttta agggaaaact ctatttactt acaatcctta
      781 caacattatc catgctcgct acaaaaagaa ttcctgtgac cgaagccgta tggattgagc
      841 tatcagaact caagtcggca ggacagacct attctcaact cctggaagag atgattgaag
      901 atagaaagaa ggctcgacta ttcagagata tggaaaagat tgagaaagag ggtgagtttg
      961 tggagcttcc gtggtaacct ttcatattat gatcaataag gacgctttgg agttcatcaa
     1021 ctcgttgccc tcaaagagcc atcgaatcgt ggtcgaaaaa tgcaaaacac ttgcaatcaa
     1081 tccatttcct ggccaaggag acaaagaatt tattcagcga aaggaccatg aagacattta
     1141 taggctgcat atttcaaggt cgtataccgc tttttataaa atacataaaa atgaaaaaat
     1201 tgtcaggatt ttggacatca tgactattga gcaagcacac aagatatacg gtcgattttg
     1261 atatgcgtgg atgcttgtat ttcgaatttc tttgtattat tcgagtcgcg atgccctaat
     1321 cgtgacgaca tgcaaaaaaa ggattttgga tcgcaaatgc aggatcttta aaagtcggtc
     1381 atgatcctgt actgatcgat ctcagtctgt ttcaggccgg ttaagaactg ctcagccatg
     1441 tcgcgaatat ccctggacct ggttatcgct cggcctacca ccagtatgtc cgcgcccgcg
     1501 gccagggctg caccctgggt atcggtgcga attccgccag caacggccac cagaagcctt
     1561 ctcccctcct ctgccaaggc cttgatatcg gggatgttct gccaggcatg agcgctcttc
     1621 tccatgtcga tcgcccggtg cagctc
//
LOCUS       DAOSGP010000306         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_77671_length_1625_cov_21.785350, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000306 DAOSGP010000000
VERSION     DAOSGP010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77671_length_1625_cov_21.785350"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PLV49_07210"
     CDS             complement(<1..806)
                     /locus_tag="PLV49_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOG98666.1"
                     /translation="MVAIKIGFVSRREEESVLLAERLMDEIESWNSGVRILADEDLAL
                     KIGRPPSSVQEMERQKVDFIVSIGGDGTILRTIHKMADPVPILGINMGTLGFLVDVEP
                     ADALETIKRLLSGFVVDERSRLKLLLNGVCMPRATNEIAFITASPAKMIEFEILVDGS
                     LMEDFRADGVIIATATGSTAYAMSAGGPIVDPRVDAIVLVPMAPFKLSSRPWVMPGNS
                     VIEVRLKLPEKEALVVVDGQSSTNISTKDSIVISKAKTPARFVKAAKDGF"
     gene            complement(793..1611)
                     /locus_tag="PLV49_07215"
     CDS             complement(793..1611)
                     /locus_tag="PLV49_07215"
                     /EC_number="3.1.3.11"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
                     /protein_id="HOG98667.1"
                     /translation="MSELLSTSGNRLLGLCDDASRAVASSIRDMIGTASGGQMVNMGA
                     DGTPTKVIDRAAENAVLDVLQSSGMGFLVLSEERGEVRIGENPDHFLHLDPLDGTFNA
                     ISGIPFYALSIYISGHDCRLGYIYDLARGQRFYAEAGRGAYAGSGERICVSRNEDFKN
                     FSISAYTIRPNTGRITGIGNRVRRIRTLGSASLEMALVASGKLDAFVDLRGMMRVVDV
                     AAGILIIEEAGGRVSDSDGNQLQMAGDMWQKRCLVGSNGRRHEDLLKLIDGGCD"
BASE COUNT          342 a          526 c          409 g          348 t
ORIGIN      
        1 taaaagccgt cctttgccgc cttcacaaac ctggccgggg tcttggcctt gcttatcact
       61 attgaatcct tagtggatat gttcgtcgag ctctggccgt caaccaccac cagcgcctcc
      121 ttctcaggca gcttgagccg gacctcgatg acgctgttcc cgggcatcac ccaggggcgg
      181 ctggagagct tgaagggggc catgggaacc aggacgatgg catccacccg ggggtcgacg
      241 atgggccctc cggcggacat ggcataagcg gtggagccgg tggcagtggc tatgatcacc
      301 ccatccgccc ggaagtcctc catcagagag ccatccacca ggatctcgaa ctcgatcatc
      361 ttggccgggc tggcggtgat aaaggcgatc tcattggtgg cacggggcat gcagaccccg
      421 ttcaatagca gcttcagccg tgaacgctcg tccaccacga atcccgaaag caggcgcttt
      481 atggtctcta gggcatccgc cggctccaca tccaccagaa agcccagggt gcccatgttt
      541 atccccagta ttggcaccgg atctgccatc ttatgaatag tcctgagtat ggtgccgtct
      601 cctcctatgg agacaatgaa atccaccttt tgccgttcca tctcctgaac ggatgagggc
      661 ggccttccaa tctttagggc gagatcctcg tctgccagga tccgcacacc gctattccag
      721 gactcgatct catccatgag cctctctgcc agcaagacag actcctcctc cctgcgggag
      781 acaaatccga tcttaatcgc aaccaccgtc tattagctta agcagatcct catgccgccg
      841 gccattggag ccgacgagac acctcttctg ccacatgtct cctgccatct ggagctggtt
      901 tccatctgaa tcagacaccc ttccacccgc ctcctcgatg atcaggatac cagcagccac
      961 atccaccacc ctcatcattc cccgcagatc gacgaatgca tccagcttgc ccgaggcgac
     1021 cagcgccatc tccagggaag cgctccccaa ggttcgaatc ctcctcaccc tgtttcctat
     1081 tccagttatg cggccggtgt taggcctgat ggtataggca gagatgctga aattcttgaa
     1141 gtcctcattc cgggatacgc atatcctctc tcccgatccg gcataagccc ctctgcccgc
     1201 ttcagcatag aatctctgcc ctcgggcgag atcataaatg tatcccagcc ggcagtcatg
     1261 cccgctgatg tagattgaca gggcgtagaa tggtatgcca gaaatggcat tgaatgttcc
     1321 gtccagggga tcgagatgaa ggaaatggtc gggattttcg cctatcctca cctctcccct
     1381 ctcctcgctc agcaccaaaa agcccatgcc cgacgactgc aacacatcca ggaccgcatt
     1441 ctctgctgcg cggtcgatca ccttggtggg agtgccgtct gcacccatat tcaccatctg
     1501 cccgccggac gcggtgccga tcatatcccg gatagaggag gccacagctc tagaggcgtc
     1561 atcgcaaagc cccagaagtc tgttgccgga ggtggaaagt agctcgctca tccctcaaga
     1621 tgtgg
//
LOCUS       DAOSGP010000307         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_78748_length_1611_cov_20.207584, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000307 DAOSGP010000000
VERSION     DAOSGP010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78748_length_1611_cov_20.207584"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1247)
                     /locus_tag="PLV49_07220"
     CDS             complement(63..1247)
                     /locus_tag="PLV49_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOG98668.1"
                     /translation="MSYERPLKSVILAELSRPAPVIHVIIGPRQVGKTTIARQIQDSI
                     GIPTIYATADSPIPLDAGWVETSWRRAVAEGAAQGFPVLLILDELQKVKGWSESLKLL
                     WDNRPPGQDIRVLILGSSALLMQEGLTESLAGRFFLHRCTHWGYPECRDAFGWTLEQW
                     IYFGGYPGASVFMENEATWKSYINDSLIETVLARDVLQMSRITKPALLRHLFALAATM
                     PAHVISYNKMLGQLQDAGNTTTLAHYLKMLENAFLVSGLELFSRGRLRKKGSSPKLVL
                     WNNALVNSLSTRGFTESLGDTSWWGRLVENAVGASLCNTLLSVEYTISYWRDGDHEVD
                     YVISRGQDVWAVEVKSGRGSKTSGLSRFRTRYPDARVLLVGGQGIPLEDFFSRDIGVW
                     LI"
BASE COUNT          345 a          468 c          416 g          382 t
ORIGIN      
        1 cgggttatcg gttcggagaa aatctacttt tccccctcca catcaatcct cacatgtcta
       61 tgtcaaatca gccatactcc gatatccctg ctgaagaaat cctcaagcgg tatcccctgg
      121 ccgcccacga gcagcaccct ggcatcaggg tatcgtgtcc gaaagcgcga gagtcctgat
      181 gtcttgctgc ccctgccgct cttcacctcg acggcccaga catcctgtcc cctggagatc
      241 acatagtcca cctcatggtc tccatcacgc cagtatgaga tggtgtattc cacggatagg
      301 agggtattac acaagctggc acccactgca ttttcgacca gccttcccca ccatgacgtg
      361 tcaccgagcg actcggtgaa ccccctggtt gatagagagt tcaccagggc attattccag
      421 agaaccagtt tcgggctgct gcctttcttg cgtaatcgtc cccttgagaa gagctccagg
      481 ccgctgacca ggaaggcatt ctcgagcatc ttgagatagt gcgcgagggt tgtggtgttg
      541 ccggcgtcct ggagctggcc gagcatcttg ttgtacgaga tcacatgggc cggcatggtg
      601 gcggcgaggg caaacagatg gcgcaacagc gccggcttgg ttatcctgct catctggagc
      661 acatcccggg ccagcaccgt ctcaatgagg gaatcgttga tgtagctctt ccaggttgcc
      721 tcgttttcca tgaacaccga ggcgccggga taacctccga agtagatcca ttgctcaagg
      781 gtccagccaa aggcgtcacg gcactccgga tatccccagt gtgtgcagcg atgcagaaag
      841 aatctgccgg caaggctctc cgtcagcccc tcctgcatca gaagggcgga tgatccaagg
      901 atgagaacgc ggatatcctg acctggaggc cggttgtccc agagcagctt caggctttcg
      961 ctccagcctt ttaccttctg cagctcatcg aggatcagga gaaccgggaa accttgcgcc
     1021 gccccctctg cgacggcccg ccgccaggat gtctccaccc aacctgcatc cagggggatc
     1081 gggctgtcgg ctgtggcata aatggtcgga atgccgatgg agtcctgaat ctgccgggca
     1141 atggtggttt ttcccacctg cctggggccg atgatgacat gaatgaccgg tgcaggcctg
     1201 gagagttcgg caagaataac ggatttcagg ggacgttcat acgacatggg ttatctttag
     1261 cacactgtat ttactagtac aagttaattt gctcaatggc tgagtatttt tactcactcc
     1321 aagagatctt gagtgcatac atctaaagcg cccagggtta atcctcagga ataaaccccg
     1381 tgccctatgc gtccaaggag attcaatacc tcctctgcca cggaccgagt catgacacat
     1441 tccgcaggtg gttttcctcc cagggcctta tgaggctctt tcatccactc cctggcttta
     1501 tccctgctct tgaaaatctc tgtccctctc tcgatcagat ctttcttagt tatccggagc
     1561 gccatgaggc ttattatggt taatggagat ctttcatgca actattgata c
//
LOCUS       DAOSGP010000308         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_79718_length_1598_cov_18.020739, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000308 DAOSGP010000000
VERSION     DAOSGP010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79718_length_1598_cov_18.020739"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..>1598)
                     /locus_tag="PLV49_07225"
     CDS             complement(468..>1598)
                     /locus_tag="PLV49_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG98669.1"
                     /translation="LKVSSRRLEGNEGKVELHFAVSDTGIGISPENQSELFQSFTQVD
                     SSKTRSYGGTGLGLAISRGIVERMGGRIWAESEVGTGSSFHFEFAADVADISADLQDL
                     DGGSPTPRSRLENKRALIIDDNGSALEMLIRATRSLGMITNGASSLQEGLSHLDRVDY
                     DVVLMDAMITDEDGQDLAEKIKSKRYGEVWLVLLSPLGRGSSSKVVADGWLTKPVKAL
                     QLRNLLEDLILHSENETPIVENNTFLDGPNTEARPIRILMAEDNPVNQKVALSMLKRL
                     GYQADVAENGLKVLQALREKPYDIVLMDVQMPEMDGLEATRRIRDSGYNTCIIAMTAH
                     SMGENRDECLDAGMNAYISKPIEIEELARVLESCIQGCAIAG"
BASE COUNT          357 a          457 c          394 g          390 t
ORIGIN      
        1 atgagcgaga aaagcttgac ggctttgctc tcaaggccat aggtatcgga cgtatctcac
       61 catagatacg ccgctagacc aataagacct gagaggtgct gttatcggga cagcatccga
      121 aaaagcacca aaaaggccga taagaccccg caggtcaaag agatacgcgc cttgatcggc
      181 gtagtatgcc ttaatcggct taatatggga aatatcgctc cggctttatg gcgcggcaca
      241 atgccgcagg cagtgcaatt gaatgacccg gcatctggcg atgatttcgg gttcctgtaa
      301 aaaggacgct gtgtaagggc ccagtgcgta gattgcattg tagcccggca ttgaatcggt
      361 ctcggggcgt gggcaggctg tcacaatgtg attagctcct cctgaaaggg cgctgtggat
      421 cggcgctggc ccgtccgaga ctcgattttt ctgtgaaatc cgaagatcta tcctgctatc
      481 gcacaaccct ggatgcatga ctccagaaca cgtgccagct cctctatctc tatgggcttg
      541 cttatgtacg cgttcattcc cgcgtccaaa cactcgtccc tattttcgcc cattgagtgg
      601 gcagtcatgg cgatgatgca ggtgttgtat cctgaatctc gaatgcgacg ggttgcctcc
      661 aggccgtcca tctccggcat ctgcacatcc atcaagacga tatcgtaagg cttctcccgc
      721 agggcctgca agaccttcaa tccattctcc gccacatctg cctggtatcc cagccgtttg
      781 agcatgctga gtgctacctt ctggttgacg ggattgtcct ctgccatcag aatgcggata
      841 gggcgagctt cagtattggg accgtccagg aaagtattat tctctactat tggcgtctcg
      901 ttttctgaat gcaatatgag atcctccaga agattgcgga gctgaagagc tttcaccggc
      961 ttggtcagcc agccatcggc cacaaccttg gaactagagc cccgacccag gggtgagagg
     1021 agaaccagcc agacctctcc atatcttttg ctcttgatct tttctgccag atcctgaccg
     1081 tcctcatccg ttatcatggc atccatgagc acgacatcat aatccactct atcaaggtgg
     1141 ctcaatccct cctgcaggct cgaagcccca ttggtgatca ttcccagcga ccgggtggcg
     1201 cgaatcagca tctctaaagc agaaccattg tcatcgatga taagcgccct cttgttctcc
     1261 aatctcgatc gaggggtagg tgaaccgcca tcgagatcct gtagatctgc tgaaatatct
     1321 gccacatcag cagcaaactc gaagtgaaag gagcttcccg ttcccacctc gctctccgcc
     1381 catatcctcc cacccattcg ttccacaatt ccccgactga tggctaatcc caggccggtc
     1441 cctccatagc tcctggtctt tgaggagtca acctgagtga aggactggaa gagctcgctt
     1501 tggttctcag gggagatgcc tattcccgtg tcgctgactg caaagtgaag ctcaaccttg
     1561 ccttcattcc cttcaagcct tctggagctg acctttag
//
LOCUS       DAOSGP010000309         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_81149_length_1580_cov_18.696393, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000309 DAOSGP010000000
VERSION     DAOSGP010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81149_length_1580_cov_18.696393"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..>1580
                     /locus_tag="PLV49_07230"
     CDS             271..>1580
                     /locus_tag="PLV49_07230"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOG98670.1"
                     /translation="MALQTRNKEEVFEAIDEFNVRFVRIWFTDILGIPKSFAINPNEF
                     DGAFSEGMGFDGSSIEGFARIHESDMVAKPDPTTFQLIPWRQQENAVARMFCDIQNPD
                     GTPYEGDPRNVLKRNLSRIKDDGYSFLVGPELEFFYFKGENNTDVLDNGGYFDLTTAD
                     NATDLRRDTILALDSMGIDVEYSHHEVAPSQHEIDLRYKDALSMADTVMTYKITVKEI
                     ARRYGYHASFMPKPIFGQNGSGMHVHQSLFQGKRNVMFDPGNEFNLSDEGRYYIAGLL
                     RHAPEITAVTNQWVNSYKRLVPGYEAPVYIAWARRNRSSLVRVPMYKPGKEAATRVEY
                     RSPDPAANPYLAFSVMLAAGLAGIANKYDLPSPQEQDIYHMTDEQRAKAGITSLPGSL
                     NDAIHITEKSTLVREALGEHVFRAFIANKKAEWDAYRMNVTQWEL"
BASE COUNT          413 a          444 c          377 g          346 t
ORIGIN      
        1 agcattcttg aataagggct aagtggccgg gcgcatatag tccattttcc cattcttttt
       61 tttcattact cgcgctttta aatcccagag cattttcttt ccgatcactt tttattatga
      121 gttattcata gccatcatat tccttttcaa tacaattgca cgccttagaa atgaacaaca
      181 tgcgatcata aattttcata atttttctgc cttattctca aatatctata aattccgctt
      241 aacgttacct tctatttgga ggtaagaaaa atggccctgc aaacaagaaa taaagaggaa
      301 gtctttgagg caatagacga atttaatgta agatttgtcc gaatttggtt tacagatata
      361 ctgggaatcc ccaagagctt cgccatcaac cccaatgagt ttgatggagc attctcagag
      421 ggcatgggct ttgatggctc atccatcgag ggcttcgcca gaatccacga gtcggatatg
      481 gttgccaagc ccgatccaac caccttccag ctcatcccct ggaggcagca ggagaacgcc
      541 gtggccagga tgttctgtga catccagaac cccgatggca ccccgtatga gggcgacccc
      601 agaaatgtgc tcaaaaggaa cctcagccga atcaaagatg acggctatag cttcctggta
      661 ggcccggagc tggagttctt ctacttcaag ggagagaaca acaccgatgt actggataac
      721 ggcggatatt tcgacctgac cacggcagac aacgccaccg acctgagacg agacaccatt
      781 ctcgccctgg actccatggg aatcgatgtg gagtacagcc accatgaggt cgctccctct
      841 cagcatgaga tcgaccttcg gtacaaggat gccctctcca tggcggacac ggtgatgacc
      901 tacaagatca ccgtcaagga gatcgcccgc agatacggct atcatgccag cttcatgccc
      961 aaacccatct tcggccagaa cggcagcgga atgcacgtcc accagtccct cttccaggga
     1021 aagaggaatg tgatgttcga tccaggcaac gagttcaatc tatccgatga gggcagatac
     1081 tatatcgccg gccttctcag gcacgctccc gagatcactg ccgtcaccaa ccagtgggtt
     1141 aactcctaca agaggctggt ccccggctac gaggctcctg tctacatcgc ctgggcaagg
     1201 aggaacagat cctctctggt cagagtccca atgtataagc ccggaaaaga ggcagccaca
     1261 agggtggagt atcgcagccc ggatccagca gccaacccct acctggcctt ctccgtcatg
     1321 ctggccgcag ggctcgccgg catcgccaac aaatacgatc tcccctcgcc ccaggagcag
     1381 gacatatatc acatgactga tgaacagagg gcgaaagcag gaatcacgtc ccttcccggc
     1441 agcctgaacg atgccatcca catcaccgag aagagcaccc tggtacggga ggctttaggc
     1501 gagcacgtct tccgggcttt catcgccaac aagaaggcag agtgggatgc ctaccgcatg
     1561 aacgtcaccc agtgggagct
//
LOCUS       DAOSGP010000310         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_82138_length_1567_cov_15.674603, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000310 DAOSGP010000000
VERSION     DAOSGP010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82138_length_1567_cov_15.674603"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..>1567
                     /locus_tag="PLV49_07235"
     CDS             171..>1567
                     /locus_tag="PLV49_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG98671.1"
                     /translation="MGSGSTELKAEYQDKEQEAGSKNRYIVLVLVLTGVLMAVLDGSV
                     VSIALPTITEHFRVSIAESQMIMTAYLVTITSLLLIFGKVAERVGKARLFMLGLALFT
                     SSSLACGISTSLEMLVLCRIFQATGGAMMFSISAAIIFLAFPKSEQGRAMGYIGSTVA
                     IGSILGPTLGGFIVDLLGWSYIFLINVPIGIVQLLLSTRYLRIEETRSKSLEVDWIGG
                     LTLIALIVSMMTFLGQLSYGFAPTPPIILLALIFVLSFFGFIINESRQKSPLLDLSIF
                     RYRMFVLPSISLILFFVANLMITVLGPFYFEGVRGYTASQVGLIFLIVPAITVFGAPL
                     TGAVYDRHQFRYLAALGMSIMALSMIALGFLAMSTANDIRQLLICFVFMGLGAAFFQS
                     PNNTELMRSLPVSKINIASSFTATIRNLGMALGVSLSGLLVSLQMAQAGYYGTISDAG
                     PAILASSISRVMMIAG"
BASE COUNT          307 a          470 c          407 g          383 t
ORIGIN      
        1 ttgacgtagc cgtgcaccgg atctcggacc tctgtcatca aagaagcctt tttaaattct
       61 agaccttaaa aatcttggct cttcgcccgt tcttaacttt tgaatgggca attgcgggct
      121 atcaactttg cggaactctt tttaagccct tggtacaagg cccttgagct atgggatccg
      181 gctcaacaga actgaaggca gaatatcagg ataaagaaca ggaggcaggc agcaagaacc
      241 gctacatcgt cctggtttta gtcctgaccg gcgtcctgat ggccgttctc gatggctcag
      301 tggtcagcat cgccctgccc accataaccg agcacttccg ggtgtccatc gccgagtccc
      361 agatgatcat gaccgcctat ctggtgacta tcacgagcct gcttttgata ttcggaaagg
      421 tggctgagcg ggtgggaaaa gccaggctct tcatgctggg gctggccctc ttcacctcca
      481 gctctcttgc ctgcggcatc tccaccagcc tggagatgct ggtcttatgc cggatctttc
      541 aggctactgg aggggcgatg atgttctcca taagcgcggc gataatcttc ctcgcctttc
      601 ccaaaagcga gcagggccgg gccatgggct acatcggatc gacggtggcc ataggcagca
      661 tcctggggcc aactctggga gggttcatcg tggacctctt gggctggagc tacatctttc
      721 tgatcaacgt gcccataggc atagtccagc tcctcctctc caccagatac ctgagaatcg
      781 aggagacgag atccaagagc ctggaggtgg actggatagg cggcctgacc ctgatcgcac
      841 tcatcgtctc catgatgaca tttctcggcc agctctctta tggctttgcc ccgacgccgc
      901 ccataatcct cttagcactg atcttcgtcc tatccttctt cggattcata atcaacgagt
      961 ccaggcagaa gtcgcccctg ctggaccttt ccatcttccg ctaccggatg tttgtcctgc
     1021 ccagcatcag cctgatcctc ttcttcgtgg ccaacctgat gataaccgtc ctcgggccgt
     1081 tctattttga gggggtgagg ggctatactg cttcgcaggt gggactgatc tttcttatcg
     1141 tcccggccat caccgtcttc ggagcgcccc tgaccggtgc cgtatacgac aggcatcagt
     1201 tcaggtacct ggcagctctg ggaatgtcga tcatggccct ctccatgatc gctctgggat
     1261 tcctggcaat gagcacggcg aacgatatca gacagctctt gatctgcttc gtcttcatgg
     1321 ggttgggtgc cgcgttcttc cagagcccca acaataccga gctgatgagg tccctgcccg
     1381 tctccaagat caacatcgcc tccagcttca ccgccaccat cagaaacctg ggaatggcac
     1441 ttggcgtctc cctctccggc cttctggtct cgctgcagat ggcccaggcc ggttactatg
     1501 ggaccatatc cgatgccggc cctgcgatcc tcgcaagcag cataagccgt gtgatgatga
     1561 tcgcagg
//
LOCUS       DAOSGP010000311         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_83439_length_1552_cov_20.319973, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000311 DAOSGP010000000
VERSION     DAOSGP010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83439_length_1552_cov_20.319973"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="mch"
                     /locus_tag="PLV49_07240"
     CDS             <1..991
                     /gene="mch"
                     /locus_tag="PLV49_07240"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR03120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HOG98672.1"
                     /translation="GKVFLLMGINEMGFEVFEEMLDYGDELQIEVNELENGTVVIDAG
                     VKAQGGIGAGIYLSRLCMSDLADIQLAPCDIGGVLLPGVQVATDYPAVSCMASQCAMW
                     QIKADKYFAMGSGPARVLAGKTRDLYEKIGFEEFSDIGVIVLESNKLPDEKVSASIAQ
                     KCGIDPADLRIAIAPTDSVAGLVQVSARVVETGLHKLFTMGFDINTIKSGWGRAPISP
                     ITGDATNCMGSSNDAIIYGGETYYTLLYEDTEELQRYLKGMPSSASRDWGAPFYQTFK
                     AAGFDFFKVDHNVFAPAKVVMNEIKSRRTFVCGKTDHEVLMESFHLECLDSSV"
     gene            1063..1317
                     /locus_tag="PLV49_07245"
     CDS             1063..1317
                     /locus_tag="PLV49_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98673.1"
                     /translation="MNSSECDLKGHGPCGGPICQRGISLNHRCDQHVRLQSYWAAHNT
                     GKKLNDWLDGLMNAEREEALRLAPPRGKHWQEISSSSSED"
     gene            complement(1328..>1552)
                     /locus_tag="PLV49_07250"
     CDS             complement(1328..>1552)
                     /locus_tag="PLV49_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOG98674.1"
                     /translation="YWPSPLKEGMVLHIGHWMQFVPARVQEVLPEADWHRAELKLKLD
                     KMLIFRPGAKAVLTYPEGGKLRVVGTIDLP"
BASE COUNT          346 a          408 c          470 g          328 t
ORIGIN      
        1 gggaaaggtg ttcctcttga tgggaatcaa tgagatgggt ttcgaggtct tcgaggagat
       61 gctcgactat ggagatgagc tgcaaataga ggtcaatgag cttgagaacg gcacagtggt
      121 catagatgca ggagtgaagg ctcagggcgg gattggagcc ggcatctacc tctcccgcct
      181 gtgcatgagc gacctggccg acattcagct cgctccctgc gatataggcg gagttcttct
      241 accaggagtg caggtggcca cggactatcc ggcggtatcc tgtatggcct cccaatgcgc
      301 catgtggcag atcaaggcag acaagtactt cgccatgggt agcggcccgg caagggttct
      361 ggcgggaaaa accagggatc tatatgagaa gataggattt gaggagttct ctgatatcgg
      421 ggtcatcgtc ttagagtcga acaagctgcc ggatgagaag gtttcggcat cgattgccca
      481 gaagtgtgga atcgatcccg ctgaccttag aattgccatt gcccccacgg actccgtggc
      541 cggcctggtg caggtatcag cgcgggtggt ggagaccggc ctgcacaagc tcttcaccat
      601 gggctttgac ataaacacca tcaagagcgg ctggggaaga gcgcccatct cgcccatcac
      661 cggagacgcg accaattgca tgggctcctc caatgatgcc ataatctacg gcggagagac
      721 ctattacacc ctcctctatg aggatactga agagctgcag aggtatctga agggcatgcc
      781 ttcctcggca tcccgggact ggggagcgcc tttctaccag acattcaagg cagctggttt
      841 tgatttcttc aaggtggacc ataatgtctt tgcaccggcc aaagtggtga tgaatgagat
      901 caagtcgcga cggactttcg tatgcggcaa gaccgatcat gaggtgctga tggagtcgtt
      961 ccacctggaa tgtctcgatt cgtcggtctg atgggagagt ggattcagtt ttgaggaaat
     1021 ccgggataat ctagataaac ctccgggcag cccgtgaaag agatgaattc ttcggaatgc
     1081 gacctcaagg gccatgggcc gtgcggcggg cccatctgcc agcgcggcat aagcctcaac
     1141 caccgctgcg accagcatgt caggctccag tcgtactggg cagcccataa caccggcaag
     1201 aagctcaacg actggctgga cgggctgatg aatgccgagc gggaagaggc attgaggctg
     1261 gctccgccca ggggaaaaca ctggcaggag atcagttcct cgtcctcgga ggactagctt
     1321 ttggccttca ggggaggtcg attgttccca ccactctgag ctttccaccc tcaggatagg
     1381 tcagaaccgc ctttgcgccc ggccggaaga tgagcatctt gtccagcttc agtttcagct
     1441 ctgctcggtg ccagtcagcc tccggcaaga cctcctgcac tctggccggg acgaactgca
     1501 tccagtggcc gatgtgaagc accattccct ctttgagggg acttggccag ta
//
LOCUS       DAOSGP010000312         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_83528_length_1551_cov_10.263369, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000312 DAOSGP010000000
VERSION     DAOSGP010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83528_length_1551_cov_10.263369"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PLV49_07255"
     CDS             complement(<1..1081)
                     /locus_tag="PLV49_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOG98675.1"
                     /translation="MNSRVRVMLSRILEIALLLALILGQAQAAGTVLTVDMKGTITPA
                     SDDIVLAALERAEAEDARALMLLLDTPGGGLAETTEILRLIDESEIPVIGYVHPEGAV
                     AWSAGTIILIGSDIAAMAPHTIIGSAQPVQFSPTGSTVPVNDSKTTNAIVAMIEEKAR
                     KHDRNTTAAREFVLSNLNLNADEAMKYRVVEHIASTPEELLGQINGTSIKDVTLITQG
                     AKLEVFEPPLNLQFLKMLSDPTIAGLLMLIGLYSLIFGFSSPGMGAEVFGLIALAMGL
                     IGLGFNVNLGAIFLILLGLGLILAELHSHTFGILAVAGLVCVIAGSILFVPTSFPEWY
                     VPGSYQRSMALALILPSLILGLFMAF"
     gene            complement(1157..>1551)
                     /locus_tag="PLV49_07260"
     CDS             complement(1157..>1551)
                     /locus_tag="PLV49_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOG98676.1"
                     /translation="VTAVTLRDVSLPESMRRAIAKQAESEREKRSRIILADGEFQASK
                     TMTDAARLYEEVPAALKLRELQTLVDIAREKTLIVVTPSSDAGTGSVAGLTAALNREL
                     SEREADRAEEERRTATKDSSLDSIRLRR"
BASE COUNT          319 a          464 c          432 g          336 t
ORIGIN      
        1 caaaggccat gaacaatccc aatatgagcg agggcaggat cagggccagg gccatcgacc
       61 gctggtagct gcctgggacg taccactccg gaaagctggt gggcacaaac aatatgctgc
      121 ctgctatcac acagacaagc cccgccaccg ccaggatacc gaaggtatga ctgtgcagct
      181 ccgccaggat cagtcccagc cccaggagaa tgagaaatat ggcgccgagg tttacattga
      241 atccgagacc gataagcccc atggccagag cgatcaggcc gaagacctcc gctcccatcc
      301 ctgggctgga gaagccgaag atgagggaat aaaggccgat cagcattaaa agcccggcaa
      361 tagtgggatc ggagagcatc tttaaaaact gcagattcag cggtggctcg aagacctcga
      421 gcttcgcacc ctgggtgatc agtgtgacgt ctttgatgct ggtgccattg atctggccca
      481 ggagctcctc tggggtggag gcgatgtgct cgaccacccg gtatttcatt gcctcatcgg
      541 cattgaggtt caggttggag aggacaaact ctctggcagc ggtggtgttc cggtcgtgct
      601 ttcgcgcctt ctcctctatc atcgccacta tggcattggt ggtcttacta tcatttaccg
      661 gcacggtgct tccggttggc gagaactgta ccggctgagc cgaaccgatg atggtatggg
      721 gagccatggc cgctatatca gagccgatca ggatgatcgt gcctgcagac caggctactg
      781 ctccctccgg atggacatag cctatgaccg gaatctccga ctcatctata agcctcagga
      841 tctcagtggt ctcggccaga cccccgcccg gcgtgtccag gagaagcatt aaagcccggg
      901 catcctctgc ctctgccctc tccagggcgg ctaagacgat atcgtcgcta gcaggggtta
      961 tggtcccctt catatccacc gtcaggacgg tgcctgctgc ctgtgcctgg ccgaggatga
     1021 gggctaagag caaagcaatt tccagtatcc gagatagcat gacccgtacc ctgctattca
     1081 tacaatcctc attcatttga tcctgatcgg caagtcaggc cttgagaacc caggccggcc
     1141 tgcctttcgt ggccattcat ctcctgagtc tgattgaatc cagagaggaa tccttcgttg
     1201 ccgtccttct ctcctcctcc gcccggtcgg cctccctttc cgatagctcg cggttcaggg
     1261 cggcggtcag gccggctacc gatcccgttc cggcgtcact gctgggagtg acgacgataa
     1321 gcgtcttctc ccgagcgata tccaccaggg tctgcagctc cctgagctta agggcagcgg
     1381 gcacctcttc atagagccgg gcagcatcgg tcatggtctt ggacgcctgg aactcgccgt
     1441 ctgccaggat gatgcgtgac ctcttctccc tctcggactc cgcctgcttg gctatagctc
     1501 ttctcatcga ttcaggcaga gatacatccc ttaaggtgac tgcggtaacc t
//
LOCUS       DAOSGP010000313         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_84186_length_1543_cov_17.864247, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000313 DAOSGP010000000
VERSION     DAOSGP010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84186_length_1543_cov_17.864247"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>1543
                     /gene="argS"
                     /locus_tag="PLV49_07265"
     CDS             68..>1543
                     /gene="argS"
                     /locus_tag="PLV49_07265"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG98677.1"
                     /translation="MFLDFMSEVEGLLKSALDRCGFAPEEGINSLGLELTPHADLASS
                     VAFRLAPVLKKSPAAIAGEIHQAIPHEFRYVEKVELAGPYLNFFMNRAFLNAVLNQAL
                     ADNAWTGRMNEKVIVEHTSANPDGPLHVGHIRNSVIGDTLVRILRRAGCSVDAQYYVN
                     DMGRQEAMVVVGCDHFQLDSIKADHAIARVYIAANKEMESNPAIREEADRVIQLYEAG
                     DPEVTAKVQSAVRYAIGGIEQTLQRMNIRHDSYHWESEFVRDGSVAEIVARLEKTGLV
                     AWDEGSLQLDLNDYGFEKKMVLKRANGTSLYITRDLAYHRWKSANSDRSVDILGADHK
                     LVSGQLRVALGLLGVREPEVVIFEFVSLPTGSMSTRKGKFISADELLDEVEKQAMEEV
                     RARRPDESPEFLKEVARQVASGAVRYDVVKVSADKATTFDWKQALDFEKLSAPFIQYS
                     HARACSILRKGEQSEKFQADLLVSDYEVALIKKIAQFDLVID"
BASE COUNT          337 a          422 c          466 g          318 t
ORIGIN      
        1 aaatcgcaac gtaatcctta tatcgattca tcctttcaga ggactcagat ctaattatgg
       61 tgtttccatg tttcttgatt ttatgagcga ggtcgagggc ctgctgaaaa gcgccctcga
      121 tcgctgtggc tttgcgccag aggagggtat aaacagcctg gggcttgagc ttacccctca
      181 tgccgatctg gcctcttcag tggccttccg cctggcaccg gtcctcaaga agagcccggc
      241 cgccatagct ggagagatcc accaggcgat cccccatgaa ttccgctatg tagaaaaggt
      301 ggagcttgcc ggcccctacc tcaacttctt catgaaccga gcgttcctga atgctgtctt
      361 gaaccaggcc ctggcagaca atgcctggac tgggaggatg aacgagaagg taatagtcga
      421 gcacacctcc gccaatccgg acggtccgct ccatgtgggc catatcagaa actcagtgat
      481 cggcgatacc ctggtcagaa tactgcgccg ggcgggctgc agcgtcgacg cccagtacta
      541 tgtgaacgat atgggccgcc aggaagcgat ggtggtggtg ggttgcgatc acttccagct
      601 cgactccatc aaggcggacc atgccatcgc tcgggtctac attgccgcaa acaaggagat
      661 ggagagcaat cccgccatcc gggaggaggc agaccgggtg atccagctct atgaggcagg
      721 cgatccggag gtcaccgcca aggtccagtc tgcggtccgt tatgctatcg gcggaataga
      781 acagaccttg cagaggatga acatccgcca cgacagctac cactgggagt cggagttcgt
      841 gcgggatgga agcgtggccg agattgtggc gcgcctggag aagaccggcc ttgtcgcctg
      901 ggatgaggga tctcttcagc tggacctgaa tgattatggc tttgagaaga agatggtcct
      961 gaaaagagcc aatgggacat cgctatacat cactcgcgac ctggcctacc accgctggaa
     1021 gtcggccaac tccgatcgct ctgtggatat ccttggagcg gaccataaat tggtctccgg
     1081 acagctgcga gtggctttgg gccttttagg cgtccgcgag cccgaggtgg tgatattcga
     1141 gttcgtctct ctgcctacgg gctccatgag cacccgcaag ggcaagttca tctccgcgga
     1201 cgagctattg gatgaggtgg aaaagcaggc tatggaggag gttagggccc gccggccgga
     1261 tgagagcccg gagtttttaa aggaggtggc ccgccaggtg gccagcggtg cagtgcgcta
     1321 cgatgtggta aaggtatccg cggacaaggc caccaccttc gactggaagc aggccttaga
     1381 cttcgagaag ctttctgccc ccttcatcca gtactcccat gcccgggcct gcagcattct
     1441 tcgcaagggc gaacagagcg aaaagttcca ggccgatctg ctggtgagcg attatgaggt
     1501 cgccctgatc aagaagatcg cccagttcga tctggtcatc gac
//
LOCUS       DAOSGP010000314         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_84391_length_1541_cov_18.899058, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000314 DAOSGP010000000
VERSION     DAOSGP010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84391_length_1541_cov_18.899058"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..574
                     /locus_tag="PLV49_07270"
     CDS             95..574
                     /locus_tag="PLV49_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOG98678.1"
                     /translation="MHEILEILYENAKATPSEIAALLGKSEEEVAREIKEMEDAGIIR
                     KYITLINWEKLDQSVVFAVLELKVALQRKTGYDAVAERIARFSEVESVRLISGGHDLS
                     VTVRGKSMKDIAYFVAEKIAPLDGVQSTCTHFILKSYKEHGAILTEQPKPERLVITP"
     gene            571..>1541
                     /locus_tag="PLV49_07275"
     CDS             571..>1541
                     /locus_tag="PLV49_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG98679.1"
                     /translation="MKQQTKKQRLKNVLPWNAKDHINQSVRDMPPSGIRKFFDLVSEM
                     KGAISLGVGEPDFVTPWHVREACIYSLELGNTHYTSNKGMPELREAICQEVSRDMGID
                     FDCNEEILITTGVSEAVDLAFRATLNPGEEAVVPEPCYVAYTPDIILAGGVPKTVPTR
                     LENEFRVQAEDVARRVTEKTRSLLLSYPNNPTGAIMTRSDLEDLADVVVENDLIVISD
                     EVYAELTYSGSHTSFAQLEGMKERTIILNGLSKSHAMTGWRIGYAMGDPALVGAMTKI
                     HQYTMLCAPTMAQVAALEAMQSGKDEMLHMRHEYDLRRRLFVSGLNR"
BASE COUNT          391 a          404 c          450 g          296 t
ORIGIN      
        1 gagtattaat aactttcgat agtatatgat tatccctatt atggccgcaa gcgcaaagcc
       61 tataattgaa tagagaggga aaagatagaa tttcatgcac gagattttgg agatccttta
      121 cgaaaacgcc aaggcaacac cttccgagat cgcagcgctg ctgggcaaat ccgaggagga
      181 ggtggcccgg gagatcaaag agatggagga tgcaggcata ataaggaaat acatcactct
      241 gatcaactgg gagaagctcg accagagcgt cgtcttcgcc gtcctggagc tgaaggtagc
      301 tctgcagaga aagacgggct acgatgcagt ggcagagagg atcgccaggt tctcggaggt
      361 ggagtcagtg cggctgatct ccggaggcca cgacctgtcc gttaccgtgc ggggcaaatc
      421 catgaaggat atcgcctatt ttgtggcgga gaagatcgcc cccctggatg gtgtccagtc
      481 cacctgcacc catttcatac tcaagagcta taaggagcat ggggctatcc tcactgagca
      541 gcccaagccc gagagattgg tgataactcc atgaagcagc agacaaaaaa gcagcgcctc
      601 aagaatgtcc tcccctggaa tgcaaaagat cacatcaatc agtcggtaag ggatatgcct
      661 ccctccggga tacgcaagtt cttcgacctg gtaagcgaga tgaaaggagc gatctcactg
      721 ggagtgggcg agcccgactt cgtcactccc tggcatgtgc gcgaggcctg catctactca
      781 ctggaactgg gaaacaccca ttacacatcc aataaaggca tgcccgagtt gagggaggcc
      841 atctgtcagg aggtcagcag ggatatgggg atcgacttcg actgcaatga ggagatcctc
      901 atcaccactg gagtgagcga ggcggtggac ctggccttcc gggcgactct gaaccccggc
      961 gaggaagcgg tggtgcccga gccctgttat gtggcttaca ccccggacat catcctggcc
     1021 ggtggagtgc caaagacggt ccccacccgg ctggagaatg agtttcgagt ccaggcggag
     1081 gatgtcgcca gaagagtgac cgagaagacc cgcagccttc tcttgagcta tcccaacaac
     1141 cccaccggtg ctataatgac ccgctcggac ctggaggatc tggctgatgt ggtggtggaa
     1201 aacgacctga tagtgatatc cgatgaggtc tatgcagaat taacctattc cgggagccac
     1261 accagcttcg cccagctgga gggcatgaag gagaggacca tcatcttaaa tggcctctcc
     1321 aagtcgcatg ccatgaccgg ctggcggata ggctatgcga tgggagaccc ggcgctggtc
     1381 ggtgcgatga ccaagatcca tcaatacacc atgctctgcg cccccaccat ggcccaggtg
     1441 gcagccctcg aagccatgca atcgggcaag gatgagatgc tgcacatgag gcatgagtat
     1501 gacctgcgca ggcgcctttt cgtctcagga ttaaaccgca t
//
LOCUS       DAOSGP010000315         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_84747_length_1537_cov_15.681511, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000315 DAOSGP010000000
VERSION     DAOSGP010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84747_length_1537_cov_15.681511"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /gene="gpmI"
                     /locus_tag="PLV49_07280"
     CDS             complement(<1..881)
                     /gene="gpmI"
                     /locus_tag="PLV49_07280"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOG98680.1"
                     /translation="MRPIAIIIMDGFGISPTQEGNAIAHARKPNLERLWKEYPTTTLK
                     ASGLAVGLPRGQMGNSEVGHLNLGGGRIVYQDLTRISLAVEKGTFASNPVLHEAMNLA
                     RKRDSKLHMIGLLSDGGVHSHITHLYALLEMARSMDLSRVFVHAILDGRDVPPRSALG
                     YFRDLEEKFSQIGTGSTATVSGRYYTMDRDRRWERVEKAYRCLVYGEGLRAESAEEAV
                     RKGYDRGENDEFIMPTVVDERGMVDDGDSIIFFNFRPDRAREITRAFVDKDFQEFATK
                     PIRVHYTCMTQYDATLNA"
     gene            complement(912..>1537)
                     /gene="pcm"
                     /locus_tag="PLV49_07285"
     CDS             complement(912..>1537)
                     /gene="pcm"
                     /locus_tag="PLV49_07285"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOG98681.1"
                     /translation="KRLFDGMRGRINESVLEAMARVPRDKFVPAQLRDRAYEDLPLPI
                     GQGQTISAPHMVAIMCDLLDIRPGMKILEVGGGSGYHAAVLAALAGPEGQVYSVERMP
                     DLALAARKNLQAAGIAGVTVVEGDGSLGLPEHAPFDRISVAASAPRVPEPLKEQLRVG
                     GKMILPVGETSQELVLVTRKNGFAVEEKMGVIFVPLIGKEGFGERQI"
BASE COUNT          303 a          470 c          406 g          358 t
ORIGIN      
        1 gcgttcaggg tggcatcata ctgggtcatg caggtgtaat ggacccggat cggtttggtg
       61 gcgaactcct gaaaatcctt atccacaaaa gcgcgggtta tctctcgggc cctgtccggc
      121 cggaagttga agaagatgat gctatcccca tcatccacca tgcccctctc atccaccacg
      181 gtgggcatga tgaactcgtc gttctccccc cggtcgtatc cttttctgac tgcctcttct
      241 gcgctttccg cccgcagacc ctcgccataa accaggcagc ggtaggcctt ctctactctc
      301 tcccagcgac ggtccctgtc catggtgtag tagcggccgc tcacagtggc cgtgctgccc
      361 gtgcctatct gagagaattt ctcctccaga tcccggaaat agccaagagc gctgcgggga
      421 ggcacatccc ggccatctaa aatggcatgc acgaatacac gggagagatc catggatctt
      481 gccatttcca ggagggcgta aaggtgagtg atgtggctat gcaccccacc gtcggagaga
      541 aggccgatca tgtgaagctt actgtccctc ttccgggcca gattcatggc ctcatgcaga
      601 acgggattac tggcaaatgt gcccttctcc accgccaggc tgatcctggt caggtcttga
      661 tagacgatcc ttcccccgcc caggttgagg tgtcccacct cggagtttcc catctggcct
      721 cggggcagac ccaccgccag gccggatgcc ttgagggtgg tggtgggata ttccttccac
      781 aatcgctcca ggttgggttt tctggcatga gcaatggcat ttccctcctg ggtggggctt
      841 atgccgaagc cgtccatgat tattatcgct attggcctca tcttgtcaga ttgatcattc
      901 agccgaatat atcaaatctg ccgttctcca aatccctctt tgccgatgag ggggacgaag
      961 atcactccca tcttttcctc cacggcaaag ccgttcttcc tggtgactaa gaccagctcc
     1021 tgtgatgtct ctccaaccgg aagtatcatc ttgcctccaa cccggagctg ctcttttagg
     1081 ggttcgggaa ctctgggagc tgatgcagca acgctgatcc tgtcgaaggg agcatgctcc
     1141 ggaaggccca ggctgccgtc gccctcaacc acggtgacac ctgcaattcc tgccgcctgc
     1201 agattttttc tggcggcaag agcgagatca ggcatcctct ccaccgaata gacctggccc
     1261 tcgggacctg caagagctgc cagcacagct gcatggtagc cgcttccacc gcccacctcc
     1321 aggatcttca ttcccggcct gatatcaaga aggtcgcaca ttatggccac catatggggg
     1381 gcagagatgg tctgaccctg gcctatgggc aggggaaggt cctcgtaagc cctatctcga
     1441 agctgcgcag gaacgaattt atctctggga acgcgggcca tggcttccag gacagattcg
     1501 tttatcctgc ctcgcatgcc gtcaaatagc cttttgc
//
LOCUS       DAOSGP010000316         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_85990_length_1523_cov_11.713215, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000316 DAOSGP010000000
VERSION     DAOSGP010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85990_length_1523_cov_11.713215"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLV49_07290"
     CDS             <1..796
                     /locus_tag="PLV49_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOG98682.1"
                     /translation="KIVQTLSGQLSWSHFIEIIYLKDPLQRQFYTEMARVERWSVRTL
                     RKKIQGMLYERTAISRKPEELARQELVALQDDDRMTPDLVFRDPYLLDFLGLEDIYSE
                     RDLEAAILRELERFLLELGTDFSFIARQKRMTIGDRDFYLDLLFYHRSLHRLVAIELK
                     LGSFDAAYKGQMELYLRWLDRYERRPDEEPPIGLILCGEKNREQIELLQLDRGEIRVA
                     EYLVELPPREMLEAKLHEAIRLAKGQVEAREEKPPGWDRHNSGSSG"
     gene            complement(1063..>1523)
                     /gene="rnhA"
                     /locus_tag="PLV49_07295"
     CDS             complement(1063..>1523)
                     /gene="rnhA"
                     /locus_tag="PLV49_07295"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF041175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOG98683.1"
                     /translation="TVEMITVYFDGLCYPKNPGGVAAYGYLVCRDKEPIWKGFGAVGE
                     GRGMTNNVAEYEGLLAAARWLNDEEIDEKIQIRGDSELVIKQMKGEYRVNSATSKRYV
                     PQIKELLVGKEVSFQWVPREKNEEADRLSRVAYESYVRRKKSGSDSVSAN"
BASE COUNT          339 a          434 c          416 g          334 t
ORIGIN      
        1 caagatagtc cagacactgt ctggacaatt atcttggtcc catttcattg agatcatcta
       61 cctcaaggat ccactgcagc gacagttcta caccgagatg gccagagtgg agcgctggag
      121 cgtgcgaacc ttgagaaaga agatccaggg catgctttat gagcgcaccg ccatctcccg
      181 taagccggag gagctggcca gacaggagct ggtggctctg caggatgatg accgcatgac
      241 ccccgatctg gtcttccggg atccctatct actggacttc ctggggctgg aggacatcta
      301 cagcgagcgg gatctggagg cagccatcct ccgagagctg gagaggttcc tgctggagtt
      361 gggtacggac ttcagcttca tcgcccggca gaagcgtatg accatcggag acagggactt
      421 ctacctcgac ctgctcttct atcaccgcag cctgcatcgt ctggtggcca tagagctcaa
      481 gctgggcagc ttcgatgctg cctacaaggg ccagatggag ctttatctgc gctggctgga
      541 cagatacgag cgtcggccgg atgaggagcc gcccataggg ctgatcctgt gcggcgagaa
      601 gaaccgggag cagattgaac ttttgcagct cgaccggggc gagatcaggg tggctgaata
      661 tctggtggag ctaccaccga gggagatgct ggaggccaag ttgcatgagg ccatacggct
      721 ggctaagggg caggtggaag caagagaaga aaagcctcct ggatgggatc gccacaatag
      781 cggttcttca ggatgaacgc ctggtcctgg agagaaatcg gaaacgcata aaaccgtatc
      841 tactaaggca tagtgctgct tcatggtggc tggatgccgg gattcatatc ggggatgtag
      901 ccgggcagct cggacatgcc agccaagctt tttgacaatg aactccttat aggcaatact
      961 gcgcccgtta cggtgtgtat cttttttgtc cctatccaaa ggatcgctct gcaactcaat
     1021 ttatcactca gcttctaagc caagcaaaag gcacaagcgg catcaattag cagataccga
     1081 atcagagccg ctctttttcc ttcgcacata gctctcatac gccacccgtg agagccggtc
     1141 tgcctcttcg ttcttctctc gcggaaccca ttgaaagctc acctcctttc ccaccagaag
     1201 ctccttgatc tgggggacat acctcttgga ggtggccgag ttcacccggt actccccttt
     1261 catctgcttg atcaccagct ccgagtcccc ccggatctgt atcttctcat cgatctcctc
     1321 gtcattcagc caccgggctg cggccaagag gccctcgtac tccgccacgt tgttggtcat
     1381 cccccggccc tcccctacag cgccaaagcc cttccagatc ggctccttat cgcggcagac
     1441 cagatagcca taagcggcta ctccaccagg atttttggga tagcagaggc cgtcgaagta
     1501 aacggttatc atttcaacag tca
//
LOCUS       DAOSGP010000317         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_87217_length_1509_cov_12.477992, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000317 DAOSGP010000000
VERSION     DAOSGP010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87217_length_1509_cov_12.477992"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLV49_07300"
     CDS             <1..242
                     /locus_tag="PLV49_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOG98684.1"
                     /translation="IPYWNGIYMFSEYIRAALSQAKYESLEDGSYMATVAGLPGVIAT
                     GDTIEKCRDDLIEVIEESVAIRLLHESKNIEEADK"
     gene            complement(309..614)
                     /locus_tag="PLV49_07305"
     CDS             complement(309..614)
                     /locus_tag="PLV49_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98685.1"
                     /translation="MAAVLSQNEVETGLLRKGFTRSNTDHRYYILRINEKKEAATFLS
                     HGKNQDIGAPLLGAMAKELGINTSEFIDLVRCPLSTEDYLARKKHRLADALRILKRG"
     gene            complement(614..925)
                     /locus_tag="PLV49_07310"
     CDS             complement(614..925)
                     /locus_tag="PLV49_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98686.1"
                     /translation="MAAQSSTIEFYEKHPLMEFFVGARRFMPKIPINLQVDYDDGLWI
                     VCLPFVDAQGSSIDYHEAKLKLFEYIDFLWSEYVSCPEEELGETGRMQRELLQEMFKV
                     C"
     gene            complement(1250..>1509)
                     /locus_tag="PLV49_07315"
     CDS             complement(1250..>1509)
                     /locus_tag="PLV49_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG98687.1"
                     /translation="ALFAMYGLVYALVDGSERAYVSDLCPAGLQGSSLGIYYGAVGVA
                     SIISSLVAGALWSVWGAEALFLYGAAAAALAAVGLLVMMKK"
BASE COUNT          394 a          385 c          368 g          362 t
ORIGIN      
        1 aaatcccata ctggaatgga atctatatgt tctcagagta catcagagca gctttgagtc
       61 aagccaagta cgaatccctg gaggatggct cttacatggc cacggtagcc ggccttccgg
      121 gagtgatcgc taccggagat acgatcgaaa aatgtcggga cgatctcata gaagtaattg
      181 aagagtcggt tgccattcgc ctcttacatg aatccaaaaa tatagaagaa gctgataagt
      241 gacaggctcc aggttggatc gctgggctga agcggcaaaa tctgccgaag cgagagatgg
      301 atggctggtc agccacgttt caatattcgc agcgcatcag ccaaacgatg cttcttcctg
      361 gccagataat cctctgttga taaagggcat ctcacaagat ctataaactc gctggtatta
      421 attccaagct ctttggccat ggcgcccaag agaggcgcac cgatatcctg gttctttcca
      481 tggctcagaa atgtagccgc ttctttcttt tcattaatgc gaagaatgta atatcggtga
      541 tctgtattgc ttcttgtaaa gcccttcctc agaagccctg tctcgacttc attctggctt
      601 agaacagcgg ccatcagcac accttgaaca tttcctggag caattccctt tgcatcctgc
      661 cggtttcgcc caattcctct tcaggacacg agacatactc actccataaa aagtcaatgt
      721 actcaaataa cttcagcttt gcctcatgat aatcgatgga gcttccttga gcatctacaa
      781 agggcaggca gacaatccac agcccatcgt cgtagtcgac ctgcaggtta attgggatct
      841 tgggcataaa tcttctcgcc cctacaaaga actccattaa gggatgcttt tcgtagaact
      901 cgattgtcga ggattgggca gccattgata ttagttggat ctgcacccta ttttaatttt
      961 ttccgcgtaa ggtgtaaggg acgcacctca tggaggattt gaatctttga gaaacctggc
     1021 tgctcgatga gcttatccgt gtcatgatgg aagagctcat gacaggcagg ctgcgggcgg
     1081 gcactgatgg aggcatagag atccggttgg ataaggtggt tggtggccgg cggtcatctg
     1141 gcccgcccag tgtatatttt gctaattata aatgcgcgcg ctctttttgg gcggcggtgc
     1201 cgggtgcaac cagctgataa aaacgtcaaa tgaatgaaaa atgtctatat cactttttca
     1261 tcatcaccaa tagccccact gccgctaaag ccgcagctgc agcaccatat agaaacaaag
     1321 cctctgcccc ccacacagac cagagggctc cggcgaccag gctggagatg atggaagcca
     1381 cccccactgc cccgtaataa atgcccagag agctgccctg aagcccggcc gggcagaggt
     1441 cggagacata cgctctctcc gagccgtcca ccagggcata aaccaggccg tacatagcaa
     1501 aaagggcaa
//
LOCUS       DAOSGP010000318         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanothrix soehngenii isolate AMR_MDS_1750
            NODE_87646_length_1504_cov_17.838509, whole genome shotgun
            sequence.
ACCESSION   DAOSGP010000318 DAOSGP010000000
VERSION     DAOSGP010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863279
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix soehngenii (wastewater metagenome)
  ORGANISM  Methanothrix soehngenii
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:52:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,441
            Genes (coding)                    :: 1,438
            CDSs (with protein)               :: 1,438
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Methanothrix soehngenii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87646_length_1504_cov_17.838509"
                     /isolate="AMR_MDS_1750"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2223"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PLV49_07320"
     CDS             <1..971
                     /locus_tag="PLV49_07320"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOG98688.1"
                     /translation="YLLIGREKRYTVPRALSTPPSRRRPWQVNMLFKGDAFDFDQDGF
                     YATLLDLHKRDIIRIDTISGTEIMVLYPSAPDLDDYERRVIEFLQDNSRGGAFSAPGF
                     EAEVKSLAKSNDTGELARLRSAMDEILHYVDKDVVSRHAVGRGIRAMGIGIQTRHLIL
                     PLIWGALFIVPFIMGSGILGDPVAITAMILLLQSSLAVSAPSTLFGRWKEDYYKEKLE
                     WDAFRTFLGDYAMIQRYSPSDLNMWKEWLIYGTALGVGDKVEKALKDFNIPIPEAVAI
                     HTIHTSFGHAYSSSSPKSSSSGGFGGGSGGGFGGGGGGGGGGGGAR"
     gene            1299..>1504
                     /locus_tag="PLV49_07325"
     CDS             1299..>1504
                     /locus_tag="PLV49_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG98689.1"
                     /translation="MLSVERTNNKGEVEVAICPLCNLQVEFMGAPMVRCYTTGETYEQ
                     DEIVWTPKSRVSKRTGYDAENIRG"
BASE COUNT          365 a          359 c          430 g          350 t
ORIGIN      
        1 tctacctgct cattggccgg gagaagagat ataccgtgcc cagggccctt tccaccccac
       61 ccagcaggag aaggccctgg caggtgaata tgctcttcaa gggggatgcc ttcgatttcg
      121 atcaggacgg attctatgcc acgttgctgg atctgcataa gcgggatatc atcaggatag
      181 atacgatatc tgggaccgag atcatggtcc tctacccatc cgcaccggac ctcgatgact
      241 atgagaggag ggtgatcgag tttctccagg ataactctcg aggaggggcg ttctctgctc
      301 cgggctttga ggcggaggtg aaatccctgg caaagtccaa cgatacaggt gagctggcca
      361 ggctgcgcag cgccatggac gagatcctgc attacgttga caaggatgtg gtttctcgtc
      421 atgcggtcgg caggggcatc agagccatgg gaatcgggat tcagaccagg catctaatcc
      481 ttcccctcat ctggggagct ctcttcatcg tcccctttat catgggttcg ggcatcctgg
      541 gagatccggt ggcaatcact gccatgattc tgctcttgca gtcaagcctg gctgtatctg
      601 ccccctccac tctctttggt cgctggaagg aggactacta taaagagaag ctggagtggg
      661 atgccttccg aacctttttg ggcgactatg ccatgatcca gcggtattcc cccagtgacc
      721 tgaatatgtg gaaggagtgg cttatctacg gcactgctct cggggtgggg gacaaggtgg
      781 agaaggcatt gaaggatttt aacatcccca ttcccgaagc ggtggccatc cataccatcc
      841 ataccagctt cggccatgcc tactcctcca gctctcccaa gtccagcagc tccggaggat
      901 tcggcggggg aagcgggggc ggatttggcg gtggcggcgg tgggggagga ggcggtggag
      961 gggcgaggta gcacctccct cccctcaccg tctgattctt taggaactat tatgagcatt
     1021 atctgcgcat cgaatttcct cttttggcgg caaattaggg gcaataaaat cctccaggcc
     1081 atatgatcag atcatgatct aaagaggcat aacgcaatcc tccagcgtta tcttcagaga
     1141 tccagaagaa aacggatatg aattattatg atcttttctt aaatagcttt aaacttaaaa
     1201 catagatatt tatggtttgg ctctggcaaa aagggccaag cttaaataag tggagatttg
     1261 gacagcccat aaaccgtggt tttaagagag tgggaataat gctcagcgtc gagagaacaa
     1321 ataataaagg agaggtcgag gtagcgatct gtccgttgtg caacttgcag gtggaattca
     1381 tgggcgcacc aatggtcagg tgttatacca ctggggagac ctatgagcag gatgagatcg
     1441 tgtggactcc caaatccagg gtctccaaga ggacagggta tgatgcagag aacataagag
     1501 gaaa
//
